LOCUS       DACDIH010000001        97577 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDIH010000001 DACDIH010000000
VERSION     DACDIH010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 97577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 97577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..97577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.001"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            264..866
                     /locus_tag="IV281_000001"
     CDS             264..866
                     /locus_tag="IV281_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP8779051.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            1007..2287
                     /locus_tag="IV281_000002"
     CDS             1007..2287
                     /locus_tag="IV281_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8779052.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            2300..3028
                     /locus_tag="IV281_000003"
     CDS             2300..3028
                     /locus_tag="IV281_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8779053.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(3097..4620)
                     /locus_tag="IV281_000004"
     CDS             complement(3097..4620)
                     /locus_tag="IV281_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8779054.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            4872..6053
                     /locus_tag="IV281_000005"
     CDS             4872..6053
                     /locus_tag="IV281_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8779055.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(6113..7741)
                     /gene="treC"
                     /locus_tag="IV281_000006"
     CDS             complement(6113..7741)
                     /gene="treC"
                     /locus_tag="IV281_000006"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP8779056.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(8192..10903)
                     /locus_tag="IV281_000007"
     CDS             complement(8192..10903)
                     /locus_tag="IV281_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8779057.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(10913..11614)
                     /locus_tag="IV281_000008"
     CDS             complement(10913..11614)
                     /locus_tag="IV281_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8779058.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            11800..12399
                     /locus_tag="IV281_000009"
     CDS             11800..12399
                     /locus_tag="IV281_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8779059.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            12480..13493
                     /locus_tag="IV281_000010"
     CDS             12480..13493
                     /locus_tag="IV281_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8779060.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            13601..14518
                     /gene="rbsK"
                     /locus_tag="IV281_000011"
     CDS             13601..14518
                     /gene="rbsK"
                     /locus_tag="IV281_000011"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP8779061.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            14546..14941
                     /gene="rbsD"
                     /locus_tag="IV281_000012"
     CDS             14546..14941
                     /gene="rbsD"
                     /locus_tag="IV281_000012"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP8779062.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            14941..16314
                     /locus_tag="IV281_000013"
     CDS             14941..16314
                     /locus_tag="IV281_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8779063.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            16671..18815
                     /locus_tag="IV281_000014"
     CDS             16671..18815
                     /locus_tag="IV281_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8779064.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFNHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(18867..19907)
                     /locus_tag="IV281_000015"
     CDS             complement(18867..19907)
                     /locus_tag="IV281_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8779065.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(20184..21986)
                     /locus_tag="IV281_000016"
     CDS             complement(20184..21986)
                     /locus_tag="IV281_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8779066.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(22000..22752)
                     /locus_tag="IV281_000017"
     CDS             complement(22000..22752)
                     /locus_tag="IV281_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8779067.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(22761..23123)
                     /locus_tag="IV281_000018"
     CDS             complement(22761..23123)
                     /locus_tag="IV281_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8779068.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(23299..24171)
                     /locus_tag="IV281_000019"
     CDS             complement(23299..24171)
                     /locus_tag="IV281_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP8779069.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(24222..24710)
                     /locus_tag="IV281_000020"
     CDS             complement(24222..24710)
                     /locus_tag="IV281_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP8779070.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(24848..25678)
                     /locus_tag="IV281_000021"
     CDS             complement(24848..25678)
                     /locus_tag="IV281_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP8779071.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            complement(25694..26302)
                     /locus_tag="IV281_000022"
     CDS             complement(25694..26302)
                     /locus_tag="IV281_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP8779072.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(26295..26612)
                     /locus_tag="IV281_000023"
     CDS             complement(26295..26612)
                     /locus_tag="IV281_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8779073.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(26768..27496)
                     /locus_tag="IV281_000024"
     CDS             complement(26768..27496)
                     /locus_tag="IV281_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP8779074.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            27655..28536
                     /locus_tag="IV281_000025"
     CDS             27655..28536
                     /locus_tag="IV281_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8779075.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(28773..29816)
                     /locus_tag="IV281_000026"
     CDS             complement(28773..29816)
                     /locus_tag="IV281_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8779076.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(29819..30487)
                     /locus_tag="IV281_000027"
     CDS             complement(29819..30487)
                     /locus_tag="IV281_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8779077.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            30629..32965
                     /locus_tag="IV281_000028"
     CDS             30629..32965
                     /locus_tag="IV281_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8779078.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(33080..33391)
                     /locus_tag="IV281_000029"
     CDS             complement(33080..33391)
                     /locus_tag="IV281_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779079.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            33572..35047
                     /locus_tag="IV281_000030"
     CDS             33572..35047
                     /locus_tag="IV281_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP8779080.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            35094..35678
                     /locus_tag="IV281_000031"
     CDS             35094..35678
                     /locus_tag="IV281_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP8779081.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            35680..36099
                     /locus_tag="IV281_000032"
     CDS             35680..36099
                     /locus_tag="IV281_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP8779082.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(36380..36664)
                     /locus_tag="IV281_000033"
     CDS             complement(36380..36664)
                     /locus_tag="IV281_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP8779083.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(37016..37726)
                     /locus_tag="IV281_000034"
     CDS             complement(37016..37726)
                     /locus_tag="IV281_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP8779084.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(37723..39195)
                     /locus_tag="IV281_000035"
     CDS             complement(37723..39195)
                     /locus_tag="IV281_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8779085.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            39333..39806
                     /locus_tag="IV281_000036"
     CDS             39333..39806
                     /locus_tag="IV281_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8779086.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(39959..40510)
                     /locus_tag="IV281_000037"
     CDS             complement(39959..40510)
                     /locus_tag="IV281_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8779087.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            40615..41145
                     /locus_tag="IV281_000038"
     CDS             40615..41145
                     /locus_tag="IV281_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP8779088.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(41628..42071)
                     /locus_tag="IV281_000039"
     CDS             complement(41628..42071)
                     /locus_tag="IV281_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8779089.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(42087..43247)
                     /locus_tag="IV281_000040"
     CDS             complement(42087..43247)
                     /locus_tag="IV281_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8779090.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            43754..45454
                     /locus_tag="IV281_000041"
     CDS             43754..45454
                     /locus_tag="IV281_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779091.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(45556..46389)
                     /locus_tag="IV281_000042"
     CDS             complement(45556..46389)
                     /locus_tag="IV281_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP8779092.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(46693..48858)
                     /locus_tag="IV281_000043"
     CDS             complement(46693..48858)
                     /locus_tag="IV281_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8779093.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTCFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            49118..51412
                     /locus_tag="IV281_000044"
     CDS             49118..51412
                     /locus_tag="IV281_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8779094.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            51405..52070
                     /gene="pgmB"
                     /locus_tag="IV281_000045"
     CDS             51405..52070
                     /gene="pgmB"
                     /locus_tag="IV281_000045"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8779095.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            52063..53070
                     /locus_tag="IV281_000046"
     CDS             52063..53070
                     /locus_tag="IV281_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8779096.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            53092..54111
                     /locus_tag="IV281_000047"
     CDS             53092..54111
                     /locus_tag="IV281_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8779097.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(54392..55759)
                     /gene="nhaC"
                     /locus_tag="IV281_000048"
     CDS             complement(54392..55759)
                     /gene="nhaC"
                     /locus_tag="IV281_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8779098.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(55868..57289)
                     /locus_tag="IV281_000049"
     CDS             complement(55868..57289)
                     /locus_tag="IV281_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8779099.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(57532..59409)
                     /gene="tdc"
                     /locus_tag="IV281_000050"
     CDS             complement(57532..59409)
                     /gene="tdc"
                     /locus_tag="IV281_000050"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8779100.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRNVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(59700..60956)
                     /locus_tag="IV281_000051"
     CDS             complement(59700..60956)
                     /locus_tag="IV281_000051"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP8779101.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            61536..62837
                     /locus_tag="IV281_000052"
     CDS             61536..62837
                     /locus_tag="IV281_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8779102.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(62906..63505)
                     /locus_tag="IV281_000053"
     CDS             complement(62906..63505)
                     /locus_tag="IV281_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8779103.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            63759..64640
                     /locus_tag="IV281_000054"
     CDS             63759..64640
                     /locus_tag="IV281_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8779104.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            64653..66263
                     /locus_tag="IV281_000055"
     CDS             64653..66263
                     /locus_tag="IV281_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP8779105.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(66340..66999)
                     /locus_tag="IV281_000056"
     CDS             complement(66340..66999)
                     /locus_tag="IV281_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8779106.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(67282..67947)
                     /locus_tag="IV281_000057"
     CDS             complement(67282..67947)
                     /locus_tag="IV281_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779107.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(68094..68699)
                     /gene="dhaL"
                     /locus_tag="IV281_000058"
     CDS             complement(68094..68699)
                     /gene="dhaL"
                     /locus_tag="IV281_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP8779108.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(68727..69710)
                     /gene="dhaK"
                     /locus_tag="IV281_000059"
     CDS             complement(68727..69710)
                     /gene="dhaK"
                     /locus_tag="IV281_000059"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP8779109.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(69712..70107)
                     /gene="dhaM"
                     /locus_tag="IV281_000060"
     CDS             complement(69712..70107)
                     /gene="dhaM"
                     /locus_tag="IV281_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP8779110.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            70261..70581
                     /locus_tag="IV281_000061"
     CDS             70261..70581
                     /locus_tag="IV281_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP8779111.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(70693..71562)
                     /locus_tag="IV281_000062"
     CDS             complement(70693..71562)
                     /locus_tag="IV281_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8779112.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(71658..72962)
                     /locus_tag="IV281_000063"
     CDS             complement(71658..72962)
                     /locus_tag="IV281_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8779113.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIANAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(72959..73468)
                     /locus_tag="IV281_000064"
     CDS             complement(72959..73468)
                     /locus_tag="IV281_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779114.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(73643..75778)
                     /locus_tag="IV281_000065"
     CDS             complement(73643..75778)
                     /locus_tag="IV281_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8779115.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(75861..77516)
                     /locus_tag="IV281_000066"
     CDS             complement(75861..77516)
                     /locus_tag="IV281_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8779116.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(77518..77832)
                     /locus_tag="IV281_000067"
     CDS             complement(77518..77832)
                     /locus_tag="IV281_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8779117.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            78002..78262
                     /locus_tag="IV281_000068"
     CDS             78002..78262
                     /locus_tag="IV281_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP8779118.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            78276..78590
                     /locus_tag="IV281_000069"
     CDS             78276..78590
                     /locus_tag="IV281_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP8779119.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(79320..79874)
                     /locus_tag="IV281_000070"
     CDS             complement(79320..79874)
                     /locus_tag="IV281_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP8779120.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(80000..81929)
                     /locus_tag="IV281_000071"
                     /pseudo
     CDS             complement(80000..81929)
                     /locus_tag="IV281_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(82235..82951)
                     /locus_tag="IV281_000072"
     CDS             complement(82235..82951)
                     /locus_tag="IV281_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8779121.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(82952..84775)
                     /gene="glpO"
                     /locus_tag="IV281_000073"
     CDS             complement(82952..84775)
                     /gene="glpO"
                     /locus_tag="IV281_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP8779122.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(84789..86285)
                     /gene="glpK"
                     /locus_tag="IV281_000074"
     CDS             complement(84789..86285)
                     /gene="glpK"
                     /locus_tag="IV281_000074"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP8779123.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(86421..87881)
                     /locus_tag="IV281_000075"
     CDS             complement(86421..87881)
                     /locus_tag="IV281_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8779124.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(87871..89166)
                     /locus_tag="IV281_000076"
     CDS             complement(87871..89166)
                     /locus_tag="IV281_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8779125.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(89498..90175)
                     /locus_tag="IV281_000077"
     CDS             complement(89498..90175)
                     /locus_tag="IV281_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP8779126.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(90205..91296)
                     /locus_tag="IV281_000078"
     CDS             complement(90205..91296)
                     /locus_tag="IV281_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP8779127.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(91397..91831)
                     /locus_tag="IV281_000079"
     CDS             complement(91397..91831)
                     /locus_tag="IV281_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8779128.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(92415..93872)
                     /gene="ascB"
                     /locus_tag="IV281_000080"
     CDS             complement(92415..93872)
                     /gene="ascB"
                     /locus_tag="IV281_000080"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8779129.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(93869..95764)
                     /locus_tag="IV281_000081"
     CDS             complement(93869..95764)
                     /locus_tag="IV281_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8779130.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(95822..97567)
                     /locus_tag="IV281_000082"
     CDS             complement(95822..97567)
                     /locus_tag="IV281_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8779131.1"
                     /translation="MDLNKRQLQILVVLRNKNHWITSEELAEALGTNKKTIQTEIKNM
                     LAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRASTIVTYLLFQ
                     KGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLKIIAEENSKRI
                     FLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSGEDFIRFSRYL
                     VLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIENRLLELNYLM
                     INDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTRMDAGHNTMNH
                     FVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFRKQGEILIVSN
                     QPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTTEQELIFEWDD
                     LIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKIHKKIDSIQQL
                     ISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKRVFFVSFDYKR
                     NDILVFFQAVSDLLG"
BASE COUNT        28705 a        20137 c        17276 g        31459 t
ORIGIN      
        1 acaattgttc attttccata agaatccctc catcaccttt tataaggtca gtaatttttt
       61 aacataaatc actcgaaaaa gcacaaactt gtcataaaaa gtacttttcg tatcaaaact
      121 gtcacttttt ttggtaaggt tcttggaaaa cgaagatttt tttaggagat catgacggtt
      181 ttgatgtcaa aaccactttt tatgaaacaa aagacgtttt ttattttttt tagcataaaa
      241 tttgtggtgg aggtacgtat gagatgaaag ataaagttgt gaacaaatta ttagaagcct
      301 tattcagcgg agacaaatcg gaagaagata tggtgtatgt ccaagtaaaa tttgtcttag
      361 aagttttatt gaataatctt ggaaaactag cggtcgttct agtcttttct ctggtgacag
      421 gctcatgggc ggaaacagga atcacctttc tttcttatat ctgcatccgc agatatgctt
      481 atggattgca ttccgactct gaattcgttt gtctgctgtg gacactactt tatttatggg
      541 gcgttccctt ggtgatgaaa catctgcaat tgactatctc cttcccaatg atggtctttt
      601 tgttaatcag ctgctttttg ttactgcttc gctatggatc aagaggtaca gcaatcaatc
      661 ccattgaacc cgagaaacgt cccccattat taaagaaggc gatcagcatg tttctaattt
      721 tttctctcat tacgttattt ttctcagctt cttacttttc tacctatttg ctacttggga
      781 ttgttttaga aatagctacg ttgcttccta ttacaaacta cttgatgaat tttggaggaa
      841 tctttcatga aaaaaacaat cattaaacaa ttaactaact tatctgactt ttttgtatct
      901 ggctgctgct ttggtatgag ttctgaattc aaaatccctg aaaaattaaa aaaataatac
      961 gccaattgtg ctataatttg gtaaaaatca atgggagctg tactttatgt ttttatggtc
     1021 catagcgttg caatatttga tttacacaat ttgtatctta ttgattgacc atcacatccg
     1081 atacaaatat ctgtttgcgg gagtcattct gatttcttgt attttgattg gatgggtcta
     1141 ttcgatttta gggattttta ctgctctaat tgctatagga atgctgatgc ttactttttt
     1201 cttcttttcg acttcgcgtc aacaattggt ttacgcgctt ccttttgggt tattgacgtc
     1261 gcttttagga gatcacttaa caagcatcgg cgattttttt gttttgaaat cttcggttat
     1321 tgaaccagga gattcattgc agtattttca cattgtctta tccgcttttt tatctgcttt
     1381 attagctttt tgtttaaaaa aatcgttaga gacttggttt cacactgtgg accgccgtat
     1441 gatcggtacg atcgggacat tgatattatt tacatactat atcattattt tttatacccg
     1501 tttttctggt gaaacacctg atgtcttagc tttgaatacg agttttttca ttctatatct
     1561 gtgtgttggc ctgatcatct tggtttatta ttggaaaatc tctaataaga aattagctga
     1621 tcagttatta gagcaacaag tcaggatgca acaggattat atcaaagatc tagaaaaaaa
     1681 ctatcaagac ttgagagaat tcaaacatga ttaccaaaat ctgctttttt ctcttcacag
     1741 ctatatttca gaaggcgact tagaaggatt acggtcttat tatgatcaaa cgatattacc
     1801 tacacgccag atgatgaacc tttttccggc caatctaagt atgttggata atatgaaagt
     1861 tcctgagatc cgtagtttac tgtcattaaa actcatgatg gcacaggaaa aagggttgat
     1921 cgtccagctt cattttcctg agccaatcga actggatacg aaacatacgg taaaccttgt
     1981 acgtatgctt ggtattatct tggacaatgc gattgaagga gctagtgctg caaagaagaa
     2041 aaaaatcgaa ttgaccatac tcaagaagaa tcattccgtg atgattcgtg tgatcaatac
     2101 aacaacaaat acattaccgt taaaccagtt gaaacaaaaa gggttctcaa caaaaacaaa
     2161 tccaaaaaac gaaggccttg gcttgtttat cttggatgaa ttgacaaaga ccaatcctta
     2221 tcttttcctt gaaacatcaa ttgaaaacca gcgtttttct caaacgattt atatacaaat
     2281 aaaataaagg agtaatgata tgattcccat ttatatttgt gaagacgatc caaaacaatt
     2341 agaagtttta tcaacggtga tcaaaaatag aatcatgatt gaaaacttag acagttatgt
     2401 ccatatttct acctctgatc cggaagaact tctcaatgca gccaagatca gaacttcttc
     2461 ttttggtatt tactttttag atatcgaact agaagacagt gaaatggatg gtgtagcgat
     2521 cggtaaattg atccgtgaat tagatccttt aggaaaaata atctatgtga cttcccatac
     2581 ggaaatgtcg atgaaaatct taaaaagcaa catcgaaccg actgattata tcgtcaaaga
     2641 agatctttat gatttaaaag aaaatgtcga gcatatcctg agcaaaattt tcgaagattt
     2701 tcaaatgtcc caaatccaaa aagatatctt caaaatagag tttaatgatg agatcaaatt
     2761 tttaccgatc aaagacatcc aatatttttc tacttcgcta ggagcgccac ataaactgga
     2821 agtccatttg actcatgccc aattacaatt ctatgagaag atcaaagaaa tcgagaaaat
     2881 gcatcggaat tttatccgtt gtcataaatc ctatgttatc aatacacaaa atgtgcggtc
     2941 gattaataaa aaaaccagag aagtcacgtt ggcaaacggc gagaccatcc cagcctctat
     3001 ccgtggatta aagaaattga tttcataaaa agaaccgaat agtttggacc gactaaatga
     3061 ccagccgatc caaacccttc ggttcttttt tgcctatcag tgcaaaagca tttcctgtgc
     3121 aatttcacgt ccatccattg tatctggata aaatgttggc caattctcca tttctttcag
     3181 caatacttct tgactttcat ttcctgcata taaatgaaaa tgttcggagt cagccggtgc
     3241 aatcgtgtga tcactgaatt gaatatatct aaaagcgcca ctttccttat cttctgcttc
     3301 gaatagatac cttacgccac ggtttcctgc ctcgtaggtc aagatttctt tccctacata
     3361 acgataattt gaacgtttcg ttgttccatt cttaaacgta aatgccatct ctttctcact
     3421 gatcttgatt gaagcaatat ctgtttgata gcccttcgta taatattctt tatattcttg
     3481 tgctgtctta tcctgattca atttttcttt atattcaaaa acaggatcaa aagtcccatc
     3541 ttctacatac gtatctactg tttgccaagt tcctgcccaa tctgaaagag agcgatcttg
     3601 tacatcctcg tcttcaaaat aaccgttgta gacgctcttt tcttttttgg gggtcaaagt
     3661 atcttctagc gggttttctg tctctgtcgt ttttttcaac gcttcgaggt ttgcttccat
     3721 gatagaaaga tagttttctc cattttctat ttgttcattc gtcaatcctt ccaaaggatt
     3781 caatacttct aaagatacac ctgcttcgtt tgctaacgtt ctggcaatac tatctttggc
     3841 gttttcttca aaataaatat agttgattcc atgttcttta acaaagtgtt tcagttcagc
     3901 aattctcgcc gcagacggtt cttcactaga agaaagacca gcaatcgata cttgattcaa
     3961 tccatattct aacgctagat agttgaatgc agcatgttgc gtgacaaaag atctttgttt
     4021 tgcttgctta aaggtttctt gataaagctg atggagtgct tgaagtttct cggtataggc
     4081 tgctgcattt tttgtccata cttcctgttt ttctgggtaa gcttcaatca gctgatcacg
     4141 tatgtttgct acctgcttga ttgctaaact tggagctagc catacatgtg ggtctagctc
     4201 atgtgtatgg ccttcttccc cgtgttcatg gtcatgatca tgtccttctt cacttcccgg
     4261 aagtaaaagc ataccttctg tcgcttttat cgtatggaca ttgccttctt gtaatgtttt
     4321 ttggattgcg gggacccatg tttccatgtt ttcatcatta taaacaaaga tatctgtatc
     4381 ttggatcttt gccatgtctt tagcagaagg ttcataatcg tgggaatcag tccctgctgg
     4441 aatcaataac tctacttctc cttcatcccc gacgattgct tttgtaaaat catacatggg
     4501 ataaaaggtc gtcatcacgc tcaatttttc tttactgtcc tcggctttcc cacttgcccc
     4561 gcaagcagtt aacatacctg ctaaaaccag catggccaat atacctatca atcgtttcat
     4621 ttatgacact cctcttctca tcttctttca tttgaatgtt ccgattcgct tttcgcaaac
     4681 cgtaatagtt acgttttagc aacaggattt aatttatcat atggttaaaa gaactgtcaa
     4741 gcgttttctt attttaattg atgagaaagt ttatatgaat gattgacgat atttttgcaa
     4801 tcaacctcta tcattttcct tttatacccg cttttgtcta taatgaagtt atagataaag
     4861 gaggaaaaag agtgaaaaaa gaaacagata caacgaatcg tttgatccgc tttttaggag
     4921 gaaaaacctc ttactatgtt ttaggaatag ttatcttact tgcgctgatc atttattttt
     4981 tccatcagat ctcgtttata ttcaagcctg tcggaatcat tatcgctact atcttgcccc
     5041 cattattgtt tgcactgatc ttgtattatc ttttggaacc agtcatcaaa cgtttgagca
     5101 agcacctttc gaggaacttg tcagtcatta tcgtttatgt gattatttta tttttattgg
     5161 ggcttggaag catatggctt attccttttc ttgaagaaca ggcaaagaca ttgatcagta
     5221 gtttgccaga tttgtttcaa gattttcaag agagtctccg acaatttttg gagaaaacac
     5281 cttttgccga ttcatttaat caatttttcg cttctattga cgacgtgacg tctgatatag
     5341 ctggatttat tggaaattat tgggaaagtg gcgcacaacg gatcagcaac attttttcaa
     5401 ctgttacagc tatttttatt actttattta ctggtccaat tgtcgctttt ttccttttga
     5461 gagatcctca aaaattttat cagtcagttt tagcgattgt cccaccagca tttcgtacag
     5521 attttaaaaa tttgacaaag attgctaatc aacaattggg ttcatttcta aaggggcaaa
     5581 tcattgcttc atttatcctt ggagcagtct actgggtttg tttcttactg atcggattag
     5641 aatttgctag tgttttagct atttctgctg ggttgttatg tatcattcca tatattgggc
     5701 cgtttatcgc cttctttcca ggtttgatca ttgcattcca agactctact tttatggctg
     5761 tcaaatttgt cattgtctgg tttgccgtac aacttctcca cggtgacttg gtgattcctc
     5821 gagtaatggg tgatcgttta cagatccatc cgattacgat cttaatcgtt ttacttgtca
     5881 tgggtgatct aatgggaatt gtgggcgtta tttttggtat tcctatctat acgttagtca
     5941 aattactcgt tacttttatt ttccgtaaat tcaaacaacg ctacaataaa ttttatggcg
     6001 ataaaggaga gtatgaaaac tcttcatttt ctgaagaaga ttattttaaa taaaacacaa
     6061 aacgtcgatc ttacgagaat agctcgtagg aacgacgttt tgtgtttttg ttttaatttt
     6121 tcagcaaagc aattgcctgg tatggtttta gctccagact ttgatgaaga acagtatcgg
     6181 aataattatc gataagaata ttggcttctg tgaatccttc tgggatggcg atccttgtat
     6241 ctttatcata gaaatttgtt aatacaagca gctgctgtcc ttcaaattgg cgaagaaagc
     6301 cataaacttc cggatgattt gcttcataag catgataatc tccttgtgca acgattggat
     6361 atgtttttct cagccgaatc aatttcttgt aataggacaa aatagaatct tcagaagata
     6421 gttctttttc tacattaatc tcttctagct tgcctccaac ttttaaccaa ggtttccctg
     6481 tagtaaatcc tgcttgttct gtgtctgacc attgcatggg tgttcgagag ttatcgcgag
     6541 attttgcttt gatccgacga aacgcttctt tttctgtgaa tccttgttca aggagtaact
     6601 tataagcatt caaactttct acgtcaatat agtcggaaat cgatgaatag tctgggtcga
     6661 tcatgccgat ttcttctccc atataaatat agggcgtccc acggttcaaa tggatcatgg
     6721 ttgctagcat ctctgctcct tgttttcgat actgatcgat agctacaaat cgattgagcg
     6781 cacgtggctg atcatgatta ttaaaaaata gagcattcca gccgttaccc tcactcatct
     6841 tttctcccca tgtatgaaat agtcgcttta attctgcaaa atcaaatgga ggagtagacc
     6901 atttattccc attttcataa tcgactttca aatgatggaa attaaagacc attgataatt
     6961 cttctctact aggttctgta tacaagatac aattttcgat cgtcgtagag ctcatttcac
     7021 cgactgtgat gatagtggaa tccgtcccaa acgttgcttg gttcaattct tttagatacg
     7081 tatgggtgat tggtttgtcc gtatattcag gtttgccatc attttcttta ttatttttca
     7141 attgttcgtc tttaccgata acattgataa catcgaaacg gaatcctttt accccttttt
     7201 ccatccaaaa acgaacaact ttgaatagtt cctctcttac tgcttggttc cgccagttca
     7261 agtcagcttg tgtccgatca tataagtgca aataatattt ccccgtatct ccaaatggcg
     7321 accaagcact tccaccaaat tttgataccc aatccgttgg ctcttctctt aaaatgaaga
     7381 agtcctgata atgctggtcc cctgctagtg cttttttgaa ccattcatgt tctgtcgaaa
     7441 cgtgattcag caccatatcg agcatgattt ctatccggta tttctttgct tcggctatta
     7501 gctgttcgaa ttcctccatc gtaccaaaga taggatcgat ggctgtgtaa tcagagatat
     7561 cataaccgtt gtctttttga gggcttggat aaatagggtt tagccaaatc atgtcgatgc
     7621 ctagatcatt cagatacggg agtttttctt tgattccttt taaatctcca attccatcac
     7681 cattactgtc tttgtaagat ttgggataga tttggtaaat gactttgtct ttaaatgtca
     7741 tggatattta cctccattca actgatttat tacctaccac aacttatata tacaagttat
     7801 catttattat tataactgaa ccgaaagcgt ttgtaaaatt tatctttata cattctaaga
     7861 ggtctgttgc ccaataaaaa acaagagatc agacatgtat ttgtctatcc tcttgtttaa
     7921 aatagcaaaa tattcttgtt tcttctataa atggtgttca aaagctttta tctacggcaa
     7981 tcagtcttct caaatgtaaa acatgaggag ctaattttat ttgagtattc tttttgctcg
     8041 atgcaggacg gcttttttac aacctctgat tcatggtgtt caatcagctg tccttcggta
     8101 ttagctcaaa aaacggcaaa atatgaaaaa ctattttgcc gtttgtttct ctaatactca
     8161 ggtcaaaccg ctgatttcac aacctctgtt tttattcatt cgatttcaac gcatcaatca
     8221 tatcgatatg ctttaatttg aaatgggtaa caatcatcac aataaccgta aatacgatag
     8281 ttaatcctgc tgataagaca taagcccacc atgtgatcac taatggaaag atgacattct
     8341 ccatagacgc ttgttgcaag atgaagtcag tcaatatata tcctacacca aatcctccga
     8401 tgattcctaa aagagtaaag atgatatttt ctcgaacaat gtacatcgtg acttctttat
     8461 caaaaaatcc aaggactttt attgtagata attcgcgtac acgctcggaa atattgatat
     8521 tagttagatt atacaagacg ataaaagcca gcagtcctga taatacgaca aagattaaga
     8581 caaccgagtc taagttcgcc attgattctt cttgcgtttc gatttgtttg gacatgaaag
     8641 acgtatttgt cacagcgtct gtggcaagca gtttctcgga aagcatttct tcttcttttg
     8701 ttgtcatctt atctgtacgc agcaaaaatg cattatccat catcgattga tcagtaactg
     8761 aagcataata ttcaggggac atataaataa agctacctag atagtttttc acgatttctt
     8821 ttacttcgac tttccattct ttttgatctt catcataaag cgaaatatga tctcctgtag
     8881 ataagtcaaa tagttctgct actttttgcg taatagcgat tcctgtttta ggcaaggagc
     8941 gtgggttctc ttcaagatca gttaaatgga taaaacgatc caaatcagct gttttacttg
     9001 gtacgatcac tgtcagactt tgcgaagcat gtcctttttt tctcaattct acggtttgag
     9061 tatgaatagc taggatctca tttacttttt cttgcttctg cagcatatcc gctgcttttt
     9121 gacgactatc ttccgtggat gaatccaatg taacgattgc ttgatagtca atgatcgttc
     9181 caaattgttt ggctgaaaca gaacttagag aatctttcaa tcccacgcct gcaaccatca
     9241 gcccggcaca tccagcaatt ccgataatcg ccataagcat tcttgactta tatcggaaga
     9301 gatttcggta actgattttt tgattaaagc taaggcgtga ccacagcgga gtgatgtatt
     9361 caagaaaaat gcgtttccct ggcttcggtg ctttcggttg aagcaatgca gcaggccgtt
     9421 ctcttaaatc tttgaataaa acgatcattg cagcgccaaa tgcggcaatg aagaatgcgg
     9481 cagctgcttg taaaatcggc ggccagtcat aaaaaataag cgcactccca atatcatacc
     9541 gctcgttaga cagctcaaaa atgatccttg gcaaaagatt cgttcctaat atcgttccta
     9601 aaacaatacc tatccctgag gctagacttg catagattgc atattttttt gcgatttcaa
     9661 ctttcgtata acctaacgcc tttaatgtcc cgatttccct gcggttttct tctaccatac
     9721 gtgtcattgt cgtaaaggtg atcaatgcgg caataaagaa aaagaaaaca ggaaaaacat
     9781 tggcaatcgc agcgatacga tcagacagat caccaaattc ttgaaaaccg acattttctg
     9841 aacgttcatt gtataggtat gatggtttag ttaaattttc aattttttct gcatttcgat
     9901 tcaattcttc ctctgcagag gccagttcac gttctttctc aactaattgc ctttcttgtt
     9961 cagcaagttg agcttcttgt tgagataatg tttgaacagc gattgttcgt tgcgcttcag
    10021 gaagctgctg gatagttgct gactgttgcg ctaactgttt tttagcatct tctaaccgtg
    10081 ttcttgcttg cgttgtttgt gctttgccat ccgctatttt ttgctgtgcc ggttctaatt
    10141 cttcgttggc cgattttttc agctcttcta agcgttcttc gggtcgatct tgtaaatact
    10201 tttctgcctt ttcttgattt tgctccattt tatctttgta agctttgctg tacgtattca
    10261 aattctttac gtttgtaaaa tcaagatata agacagaata aatatctgtt ttaaaaacgg
    10321 attcaggtaa atagacaaaa taatcgacgc taccatttcc gacgttcgcg tatcctctct
    10381 ccaaattgtt gatgaacaat ggagaacgga caaagcccgt gatggtatac tctttttctt
    10441 tcaattcatc tgaatcaatc tgataaatat caccgatttt atacccttca tcttccgctt
    10501 tttcatccaa tacaagctgg tttggttttt ccgggagttt tccatcagtt aattgtagct
    10561 tgttctgctt gttatttttg ttataagaaa atatttgaat cacttcgttt ttgccagtat
    10621 tggaataata gatctgtttt cctgtttcta cttttgcatc tgggatcttt tctgcttgag
    10681 caatatcttt ttgtgtcaag ccaccagtag aaacgatttg caaatcagaa agtcctgctg
    10741 taccaacata gtgatccatc gttttttgca tgacaggtcc agcagttttg acacctacaa
    10801 ataaaagtgt tcctagcatg acaataagga tgattgcaat gaatcgtcct ttagattgcc
    10861 gaatttcgcg aaaacctgct ttaagatatg ttcttgtttt cataaaatcc ccctaccata
    10921 caatctcatc aatggatgaa ggatgatcat ttaattcaac agaccttact ttagcatcat
    10981 taatgtgaat gactctatca gcgatgggcg ctaaagcaga attatgtgta atgatcaaaa
    11041 cggtattgcc gtgttgtctg ctagcgtttt gcaataattt caatacttgc ttgcccgttt
    11101 caaaatccag cgcgcctgtc ggttcatcac ataaaagaag tttgggattt ttagctagtg
    11161 cacgagcaat tgaaaccctc tgctgttcgc ctcctgataa ttgggacgga aagttattca
    11221 atcgatgagc taggcccact tgcctcaata cctctacagg atcaagcgca tcaggagaaa
    11281 cttccgttgc caattccaca ttttcttttg ctgttagatt aggaacgaga ttgtagaatt
    11341 ggaaaacgaa ccctacatct gttcgtcggt aagcagtaag ctgtttatct gaaaattgcg
    11401 caatatctgt gtcatcaata atgatctgac cttcatcagg tgtatccatc ccacctaaaa
    11461 tattaagaat cgttgacttt cctgctccac ttggaccaag tataatcact aattcgcctt
    11521 tttcaattga aaaagaaata tcgtcatttg ctgtaatggt cgtttctccc attttgtatc
    11581 gtttgtattc attttttact tcgacatact tcactctgat tcctccattt tcacgatatt
    11641 tctcgctata tctatgcatc gaatgcgttt tctcacactt atttatttta ccactgaatt
    11701 tctctagact aaagaaagga atcctttaat ttagttgaca aataaacgat ttatgattta
    11761 cttacataaa aaacattgtt atttgaaaga aggaacatca tgaatgattt aaaggaaaat
    11821 atgatttttg atttatcaaa aactgctttt gtcgcgatcg acatgcaaaa agagatcgta
    11881 aataacggta cactctctcc acacactgca gcatctattc tgacaaacaa tgatttgtta
    11941 gtaagaacat tgaaacgtac cgctgccttg aaagttttag tgaacgtaga catctccact
    12001 ttcccttatt tgagtcaaca aactgatatg ggtggaatga ctgcttctgt acctccagaa
    12061 ttcactgatt tacttttgaa ggattctttg aaagatacag ataatatgtt gactatcaca
    12121 aaatacaatc caagtgcttt ttttggtact tctcttgatc ttcaacttcg ccggcgtgga
    12181 atcgaaacta tcattttaag cggtgtagcg acaacaaacg gtgtatatgc tactgcatta
    12241 gatgcctttc aacacgggta ccatatcgtg cttgctgaag atgcttgcag tgatcgtgac
    12301 aaagaatccc atcaattgtt cataaaaaag atatttccga aaactgcacg agtacgctca
    12361 acgaaacaaa taatcgaagc tatccaacaa agcaaataag tactggcaca cgtgattttc
    12421 atttacttct ttttgatttc atgggaaaat aaccacagga tttagatagg agtatgaaca
    12481 tggtgaaaaa aaagatcaca atcaaagaag tcgctaaact ttccggcgtt tcaatcacaa
    12541 ctgtctcctt gatattaaat ggaaatacgg agaaattcag tccgaaaact gttcagaaag
    12601 ttttagctgc caaagaagaa ttaggctatc agcctgacta ttttgctaga caaatgatta
    12661 ccaaggaaac gaaaacaatt ggtgtactcg ttcctgatat cacgaatcca ttcttcagta
    12721 cactgatgcg aggaattgag gatatcttgt ataagcagaa tttcgtcacg atattatgta
    12781 atgctgattc tgatcatcaa aaagaaatcg aatatctagc cgagctcaca cgtcgaggtg
    12841 tggatggatt cattatcgcg acttctgctg tctctacaga tgcaatcaac gaaaatttga
    12901 aaaaacaagg ccggcccttc attgttttgg atcagaaaaa atctgaagga tttagtgatg
    12961 ccgtacgaac agacgatttt cgcggtggtt acctagcggg catgcacttg cttagtctag
    13021 gacatcaaac gatcgccctt gtctatcctg aaaatccacc ggaaaatgtc cacgctcgga
    13081 ttgaggggtt taaatctgct cttgatgttt accaaatccc tcacgaccaa ctgatcctct
    13141 tacctacaca gttctcaaaa caaggtggct accagatcac agcagaactt cttgattctg
    13201 ctgcaactgg tgtgtttgct ttaaatgatg aattagcttt tggattatat cgcggattag
    13261 aggaagctgg aaaaagcatc cctgaagact acagcattat tggctatgat aatatagata
    13321 tgtgcgagta tatcaagccg aaactgacaa cgatcgcaca accaatcttc gaattgggac
    13381 aaacatctgc caagttattg ttagatcgga tccaattccc agaaaaagaa tgggaagaaa
    13441 aaagattacc tgtacgatta gaaaaaagat tttcaactgc acccttgaaa tagctttcaa
    13501 ggtgtgctat atttttatcg aactattaaa acgttttact aaaacgtttt aaaaaataat
    13561 tttcccatca aaataaatga gatagttgga ggtttgtaaa atgaacacgg ttacagtaat
    13621 tggaagtatt aatatggaca ctacccttcg tttaaccaac atgcccaagc caggcgaaac
    13681 gatgcatgcg catgagattt ttcacgctgg cggaggtaaa ggtgccaacc aagcagtggc
    13741 agccaaacgt tcaggcgcac ggacatcttt tattggaggc gttggggctg acagcgaagg
    13801 acaacagtta ttagatctac tgacaaaaga aaacatcgat acatctggga tagctgaaat
    13861 ccaaggcgcc acaaccggac aagcaatgat tatggtagac gcagcaggag aaaatagtat
    13921 cttgatccat gcaggggcaa ataatgcttt ccatgaacaa gaagtcctaa aaaacaagca
    13981 actgattaca aacagtgact ttattattgc tcagtttgaa agtagcttag atgcgacgat
    14041 cctagctttt tctattgcaa aagacgcagg aaaaacaacg atcttgaatc ctgcaccagc
    14101 tcgtgaaacg attcctactg aactacttga aaagacagat atcattatac caaatgaaac
    14161 agaaacagaa atcattacag gaatcagagt gacagatcat aacagtttgg tcgcagcagc
    14221 cgaaaaactc catgaattag gtattggaac agtaatcatt acactaggca gtgccggcgc
    14281 tttctatcat acagaaaaag aacacggtat cgtccctgct tttaaagtgg atgccgttga
    14341 cacaacagcc gcaggtgata catttattgg cgcattaagc agcacacttc agccagacct
    14401 tagtaacttg aaagaagcga tcctttatgg aaatctagcc tcttctgttg ctgtgcagtc
    14461 ttatggtgcg caaccttcta tcccttaccg cgaagcttta gaacaatcag cagtctaaaa
    14521 ttaacaatga gaggaagaaa caaacatgaa aaaaggaaaa gtaatcaatt cagatatttc
    14581 acgagtaatc gcacaaatgg ggcactttga taaattaagt atcggggatg caggcatgcc
    14641 tgtccccttt ggtactgaaa aaatagattt ggctgtctca aacggtcttc caagtttttt
    14701 atctgtttta gaaaacgttt tagaagaact tgaggtacaa tcgattgaat tggcggaaga
    14761 aataaagaca atgaacccgg aaatattgga aaaaatcaaa ttattgttac cagatacacc
    14821 gattagcttt atcccacatg aagaaatgaa aaaagagttg aataactgtc atgcatttat
    14881 tcgtacaggc gaaatgacac cttacgccaa tattatatta gtgagtggag tggtcttcta
    14941 atggaaacga caatgaatcc tgaacagatg gtactgtccg agcagattca tatgctgatt
    15001 gaacagttgg gaggaaaaga caatattgat tctgtccttc catgtgcgac tcggcttcgt
    15061 atcaaattga aaaaacagcc agtagccaat gcagaaaaat taaaacagca aatcccttcc
    15121 attaagtatt tcattttact cgatagatat gcagaaataa tcatggatca gggattggct
    15181 cctgcttaca aggaattgac gcgtcaaatc gatcattcag ctccgaaaga aaagctaaat
    15241 ccgatcgaaa aactatttgg tttgatatcg gaaatcttta cgccgatcat tccgctgttt
    15301 gccggttctg gtatattaaa aggtctggtt gttttagcga ctacgattgg ctggcttagt
    15361 gaaacaagcg gaacttatca tattttgtct gctgcgtcga atgccgtctt ttattttctg
    15421 ccaatctttc ttgctttttc tgctgccaaa acgtttaaag tgaatggata tattgcggct
    15481 gtcattgctg cagcactgat tgaaccgaat ctgacctctt tactttcaga cagtggcaaa
    15541 gcagctactg attttctggg gattcctgta gtactgatgc agtatacgtc tactgtcatg
    15601 ccggcgattt taggtatttt cttttattct tttgttgaaa aattcctcaa aaaatatatt
    15661 cgcgaaaata tgcaattagt atttgtacct ttactttcat tagttatcac ggttcctttg
    15721 acgttaatgg tattcgggcc tgcgggtgtt taccttggcg aaggtttagc tgccgcaatt
    15781 acttggatga tggatattaa cggaccgatc agtggcgcaa tcatcgctgg tggttggaat
    15841 attttggtaa tatttggtat ccaatgggct gtaaaccctg tcatgatcag taatatctct
    15901 gcttatgggt tcgaccgaat cgttccgttg acaggagcag ccaattttgg aatggctggt
    15961 gcagctttag gtgtatttct gcgaagtaaa cgttcaaaaa cccgttcgat ctccggttct
    16021 gcttttgctt cgattttact tgcgggagtg accgaaccaa ctgtttacgg aattgcgatc
    16081 ccattgaaaa aaccatttgt tgctgcttgc attggtgcag ctgctggtgg agctgtaatg
    16141 ggatttgccc aagtgaaagc gatcgctttt gtatttggta gtctgaccac attgcctgca
    16201 ttcatttccg gtactttttt ctggtactta gcaggactgg ctgttagtct agtcgtagca
    16261 atgatcacta ctctcgttag cgggtttgat gaagacctaa tgtcttatga atagttaatg
    16321 aaaaagactg aagtatgaga tgtttatcac atatttcagt ctttttcttt ttaggatcat
    16381 tatttttata aatttttgtg atactttcca aactttggta taatttacat atgcataacc
    16441 atttttatcc ccacttgttt atccccaaac aaaggtggtc tatgcaataa aatgaacgtc
    16501 aaagaaaaac gaatgaatgc tcgttgctta tttacaaatt gttttattta gaatacacag
    16561 acagatttat gaaaagaaaa agcttttcga cacgattatc tgttccaaaa aatgtctaat
    16621 gaatgctgaa taagacagaa aatcaatgaa aggaaaagga ctagtttatt atgaaaaata
    16681 aaaaaacgat acgtttgcta gctgctactt ggctgattgc tccgtctgtt ttgtcgacca
    16741 ataccgtatt tgccgtagat aatcaaatag ctgatcaaac agtcacaaca gatacgacta
    16801 catccagctc agctactagt gaaacaacgg attcttttac agaagaccca actggaacaa
    16861 ctaactcaac aacaggatcg accgagccaa ctgaaattga tccggctgat ccatcagaaa
    16921 atgaaaagcc agccgagaca tattccctta caattgctcc ttctttgatt ggtaagatcc
    16981 aactgactgc ttcgacagcg gattcccctg aacagcaacc tttggatatt acggaaagtg
    17041 gaacggtaga aggtttatta gcggaaacga aggtaactta cactattacg ccagctgaag
    17101 gtgaacaaat tgcttcagtc gctgttaatg gtgccaaaga agtaaattat gatgcaaaga
    17161 cctttacagt aggtacgaac aatttatcta ttactgcaga atttaaagct ggaccggata
    17221 cttcaacaga gaaaccaact gatcctagtg aagaagtccc tagtacacca gatccggaaa
    17281 cgccaaacaa accagatgtg ggtaataaca acaataataa tggttcaaat aattcaaaca
    17341 ataataacaa taattcgaac agcgggaatt cttcgaatca aacaaaccca tctggatcga
    17401 ataacaatgg aaatactcag agagaaccaa accatccaaa caatggttca tcgaatggcg
    17461 gaacgaacaa tgcttcatct cgtccaaata cagcaaatca aagcggtata gagaatccat
    17521 cttctgcttc ttctgatttt gttgtaaaat caccaattga tgctgtattg cctacaaata
    17581 caacgaatgt ccaacaagca atcgttcgag aagcattcaa tcatcttgga aaaccatatg
    17641 tatggggagc aaaaggtcca aatacttttg actgttctgg tttgacttat tacgtatata
    17701 tgaaagcaac cggtcattat atcggtggct ggacaggcga acagcaatat gcaggtacgc
    17761 agattccagt aagtcaggcc caacctggcg acctcgtctt ctggggacct tcttccggtg
    17821 ttactcacca tgtaggtatc tatattggga acggacaatt tatccatgcg cctcaaccag
    17881 gagataaagt tcgagtaact tctatttcag atttcacgcc tgattttgct gtccgagtaa
    17941 acttagcagg tttacctgct gcttcaggtt cgctgacagg cggctcatct attttagatg
    18001 gattagatgg ctcgttccat ttcagtcaaa accaaacaac tgatcagttc ttgaaaaaga
    18061 ttgcagatga tgcacaggaa atcgggcaaa aagaaggaat ctacgcatca gtcatgatgg
    18121 ctcaagctat ccttgaaagt ggatctggaa acagcttatt atcaagcgaa ccaaatcata
    18181 acctttttgg tatcaaagga tcatataaag gaagcagcgt gaccttcaat acacttgaac
    18241 aagatagttc cggacaaagc taccaaatcc gcgcacaatt ccgtaaatat ccttcttaca
    18301 aggaaagttt ggaagactat gccgacttga tcaaaaatgg actgactggc aatcctgatt
    18361 tctacaaacc aacttggaag tcggaaacaa aagactacaa agaagcaacc aaatatttgg
    18421 aaggacgtta tgctacagat cgccaatatt cacaaaaatt gaacgcgatc atcgaagcgt
    18481 acgacctgac aaaatatgat gaaccgaaaa aagaagcagc aacaactgaa gaagcaaacg
    18541 ctgaaatcct caatatgtct aagcgattcg atgtgccaat caaatggaca acagatggcg
    18601 tgccaatcac aggattagaa ttttcaacga taaaacgcgt caacaacgct tcttccatct
    18661 tccgtgtcgc ttcaatctgg gatttgtgga acaattttac ggcaagacag ataccagaaa
    18721 caactactcg tacagttaaa gctgcaactg ttcctgtact aaccttgtta agtttagaca
    18781 aatcattctg gcttgatctg cctttaaaga aatagaataa attaaaaagg ggatgtgaca
    18841 ctgtcacact ccctttttaa tttattctat ttctttatat ttattttgta ttccgttaca
    18901 aactcgcgta cttggttagg ttccagagtg acatctccaa aactttcaag tcgctccgct
    18961 cctggcagtt cttgagtctc aaatgtgata ccgccatgat ttactaattt attcccgcgc
    19021 atttccggtc catcttctcc aaaatttgct gtaaaaatca cgattcctga agcatctgtt
    19081 tttacttgga tcgatacttt ttcatctggg cttgtcaatt ccgcagcatt tagtaaacca
    19141 gattgttttt tcaacaaaaa tggatgatcg atgccgccca ctatcattga ttggcgaaaa
    19201 tcactggcaa agacctcacc taatttttta ggacgttgga agtcaaatgc tgtgccactt
    19261 actgaaacta cttctcctgt aggcgttgta tctttattga gcgtagcaaa taatcggcta
    19321 ttcaaccaaa gtatatgttc gtcgattgac tgtgctggat caccggttaa attgaaatag
    19381 acatgatttg taggattgaa tactgttttg cggtcactga tcccttggac cgtcactcgc
    19441 catgtatcat cgttcgtcag cgtataccgt acttctacat ctaaagttcc aggaaatccg
    19501 ttttccatat ctgggctagt ctgatagaaa atgaccgatg cttcgttttc accattgatg
    19561 actgtataat gccactgctt ggcttcaaag ctaggtgcac caccatgtaa acaatgacca
    19621 gtgtccgggt ctgtttttac ttcgaacgtt tcatcatcta atttgaatgt gccattcgta
    19681 atccgtccag ccatacgtcc gattgaagca ccgatataag ggtctttttc tagatattct
    19741 tttgcttcat caaaacctaa gatcaactct cgtttttcat tatcgattat tgtttgaaaa
    19801 tgaacaaccc gtgccccaag atcactgaca gacaaagatg tcccattttg attcgtgatc
    19861 gtaatcaagt gatagttttc gccaaaattt tgttcattga tttccatgtt ccttgcaccc
    19921 tcttttcttt catttgcact gattccagca tacatggaaa ccgttttctt aacagcttat
    19981 tccgtgagta acgagaaaaa atttactata ttctaataaa agaccgtgaa aaaaaggttg
    20041 tgacaaaaat attgtcacaa cctctttttc cgaacaaacg gtgttcaaaa gcttgcatct
    20101 gcggtaataa gcccaaccaa acgaaaaata tgaggagcta tttttgcttg gctgttctta
    20161 ttacttgatg caggacagct ttttcacaat ctctttttca atacgattcc agtatataac
    20221 catgtcgtca gtacatagta gatgaaatac aatacgaaga atatcgtaaa cggaacccaa
    20281 atatctttgt atggattttg catgaataga ccaaacattt gtaatccgaa taagacatgg
    20341 actacaccta agattcctgg tgctaagaat aacacgccta cttctctacg gatcgattgt
    20401 ttaagcaacc cgcgtcgtgt tcctatcttt tcaagcattt cataacgagc aatgtcactc
    20461 ttgcttcctg acaagatctt gaacatcaaa cagctagcaa gcatggtcaa gaaagctaaa
    20521 cctaggaaga atcccataaa ttcaaaacca gagaatagac cattatacac ttcatacatt
    20581 tcatatttct ggttgctgta ctctactttt tgcaaagatg gattgttcat tttattttga
    20641 gcaacaagtg tttcgattgg tttcaggttt gcttggaagt cttttacctg aactagctgt
    20701 aaagtggatt gtggcaagtt cagttgatca aattctgatt gactgactac cttgatttct
    20761 ttttcttgct gttccggcaa ttcatagctt cgaagttcct cttgtacagt cagttcattc
    20821 atttttttag caggaacttt ttctttcgtg atcttcggcg gtgttccatc ttcatgtttt
    20881 atcatgatca atggttgagc cgcaaattca cttgcattat agtaaatcgt tcccgcatct
    20941 tctttttgag tataagaagc agaaagtgtt ggtgaaagtt ctttgatttt ttgctgatct
    21001 tctgcccgag gattattcat cactagatca tatccagaat tgttttctgt caagtctgca
    21061 atttcatttt taaaacctaa tcctaccgtc aaagcgccca gtgccaaggc aaacagcata
    21121 gcgaccattg agagcatttg tgtatattca cgaatacgaa agtttaactg tgacaatgtg
    21181 aaattgttca attttttcaa tgaaatctgt tctgatttct ttagtaatga taaaaagaaa
    21241 atgatcacag aatggaaaat gaaataagtt cctaatacga tcgtaactaa ggctacccct
    21301 ataccaaaga cttgataggt ccccacgtgt ttcatcatat agtaaccgca gcctagaaaa
    21361 gcaattccca gtactacttc aaggaaaaac aagaatttat gctgcttgat tcttgttggt
    21421 gtctgatccg cacgaatcaa tgctaaaatc ggttttttga caatcgaaaa agcattgaca
    21481 acagcagcta ataaaaacaa aacagcaaaa aacaaaacag tgatcaacaa cccgtgaata
    21541 ctaaaaggtg taaaatgcga aatattgaga tctaactgat ccactaataa tcggtggacg
    21601 atacttgtca atcctacacc gacagctgaa cccactaaag tagctgcgat ccctactaag
    21661 aatgtttcag aaaaaatcat ctgtgcgatt tttctgcctt ttgctcccag catcataaac
    21721 attgcatagt ctttttgccg catcgtcatc aaaaaggaat ttgcatataa aatataaacg
    21781 aatgtgatga tccctagtaa aacagaaccg aatcggaaaa tgataactgt agccgcaacg
    21841 atcgtattac ttttcaaaaa agcttcgttg cttgccattg attcaaacat gtaaaaaata
    21901 gcagatgcca taactaatcc tgagaataac accaagtagt ctcttaaacg gctcttgatc
    21961 ccagtaagtg ataatttcca aagcatagtc aatctccccc tactgttcca agtttccaag
    22021 aatagatagt atttctcgat agaaggaatc acggctttga tctccacgtt tcacttcttg
    22081 atggataccg ccatccttga tgaataaaat acgttggcaa taacttgcag aaaacggatc
    22141 atgcgtcaca agaagaatcg atacatgatc ttttgtattc aattcgtcca tcgtatctaa
    22201 aagatcccgg gcacttttgg aatccagcgc acctgtaggc tcatccccta atagaatcgt
    22261 tggttgtgtg atcaatgcac gtgcggcggc gacccgttgt ttttgtccac cggaaatctc
    22321 agaaggatag ctttccaaaa tatgagagat ggataaacgt tctgcaattt tttggacacg
    22381 ttgcttgatt tcttttggtc tgacgccttg taaagaaaga ggaacagcga tattttcttg
    22441 tgctgtcaag ctttctaata aattgaaatc ttggaaaata aagccaatct cattcgcacg
    22501 aaaatctgct agttgatttc cttttaatgt ggtcacatct ttttgattga ttcggatatg
    22561 cccatctgtt ggtttatcta atgtggataa aatattcaaa agtgtcgact taccagaacc
    22621 agaagcgccc atgatcccaa taaattcgcc tttttctaca tcgaagctga tgccgcttaa
    22681 tgcttgcgtc tttttctcat tgttttttcc gtatactttt gttacattct gtacttcgac
    22741 tattttgttc atttctttcc ctacccttta attagttttt ccagtgctgt tttcggcacg
    22801 tctttttttt cgacttcttc atacgaagtg acacctttat ttttgttcca ggtgattttt
    22861 aaatatgctc ctttacgaag cggtcgagtc aattgtccct tgaagtccag ttctttttcc
    22921 ttaccctctt tcgtatagcc aggcaggata tatctatagt tgactgtttc ttgtccagag
    22981 tctgattttg aaactgtttt tgtaccatct gtcgtaattt gtacatagta ttcttctcca
    23041 cccataacta cgtaatctgc gattttcacc cctgcaagta ccacaacgat gaaaacaagt
    23101 agaccgatca gaagtttttt cactttttcc ccttctctct tactttatat actgattata
    23161 gaaaaaagcg aacaattctg ccttcgaatc aactatcatt cttctttcaa ttttgtcatt
    23221 ttctttaaaa caactaaaaa aagagttcaa gacaataatc cgtcctaaac tccttcttcc
    23281 tatcttacaa aaagtatgtc acttacggtc tttcatgtcg cctgtattga taacaggttg
    23341 cgttcctttg tctgtaatga ttgagacaag taaattattg atcaattgga ctttccgatc
    23401 atcggtgaaa ttgatttctt gtccatcttc gatttgctct aaggccattt gagtgattgt
    23461 gacagctcct tcaacaatgg tttgtcgagc agacaaaatg gcttttgctt gttgacgttg
    23521 gagcatggca ctagcgattt ctgttgcata ggctaaatgg ttcaagcgtg tttcgattac
    23581 ttcgacacct gctactgcta aacgctcttg cagctctttt tgcaactcgt cagacactgc
    23641 cgtcgtgttt cctcgaagcg tcaaatcatt ctcattgaat gtatcatacg ggtattggct
    23701 agcgatatgc cgaatggctg tctcactttg gatttctaca aaatcttgat aatacgctac
    23761 atcgaataat gcctttgctg tatcaacgac tttaaagact actactgctg agatttcgat
    23821 ggggtttcca tccaaatcat tgacttttaa aacagaacta ttgaaattcc gaactttcaa
    23881 cgaaacagtc attttttgtg ccaaaggtat cgtaaggaaa aagccatttt ctcgaatcgt
    23941 acctaaatat tgtccaaaaa acaaaattac ttttgcttga ttaggactga ctacagttgc
    24001 cgaacttaac aacaataacg tgatcaacca aagaaaaata cttaaaaaaa gtgcccaaac
    24061 tttcgcttgc cacataccta aatagaagaa aaacaatcca actaaagcta atacgaccaa
    24121 tccgacaatc cctgcataac cattgatata aaatgctctt ttttctctca tgctacttcc
    24181 tgcgctcctt tatccttcaa taaatatcta atgttacttt tttaattttt ttttcgaata
    24241 cgcgccatta caccttctag ttctaactct ttgtcttcag tgatgtacca accgcttgcc
    24301 tgtgtccctc cggctgcctg cggtaaataa tccaattttg ttcgaaccca gtgcttttct
    24361 tctgtaaaat agtcttctac caagacctct agatccatcc ctgctgaaag aggcaaatct
    24421 tcccgaacag atttaaagat aaatcgttcc ttcttcccat catacttcaa tattcctgta
    24481 tcgataatcg gtaaagaata ataatccagc tctttcttca ccgtcaataa atgtttgaaa
    24541 acttcatggt acacttgctc taattggcga ccttctgaat tgttcgggta attaaccatc
    24601 actttttctt tgtctggatc atagaaggtt tggcttagtt gagtgatata agtatgtaat
    24661 ctttccattt ctgtttttaa cttttctctt tgaccttctt ctaccttcat atactctttc
    24721 accctgtttt ctttttagat attttctttc ttattttact atagttatgt gcgttaagcg
    24781 atgaaaaaca gcaaagattg gaaggatctt tgctgttttt tcttaaaata tccagttgag
    24841 tacaggatca atctgtttca ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt
    24901 tagcagtgat ctttagcttt ttctgtcctt gttctaacaa agtatttgtt attggctggt
    24961 agtctttttc aatataagat tcaccagacg tttggatagt atagccactg ttttccatct
    25021 catgcaaaga taaattgcca ccgtttagga tgacttttcc ttctttgctg atcaaactgt
    25081 cttctagatg gaagttgctg ttttcacctg tgacgatcaa gttatccaca ttggcctcat
    25141 ctaatcgaag tgagcctaat tcatctttcg cagttattgt tcctcctgta aattcattga
    25201 acgataccca gccatcatgt gtcttagtat tcactttttt gattgatact tcgttgatat
    25261 caattgcact attattccct tcgattgtca attcagataa ttcatgatct gcagggacaa
    25321 caatagtgat ttgtgcactt gtgtcttcta aactaatgat tgcacctgtt gattttttga
    25381 ctgcattcac agtcaacacg ccatttttct cctttatttc tggcttttct gtcttatcat
    25441 aagtcgtttt tatctcaaaa tccaatccgc gtatgaccga aactgtttga ttttctgcat
    25501 ggatgacgat tttgtttgca tccttgattt tttttgtctc agtaaccata tcgatcaacc
    25561 gaggaccatt atcccaagtc agactctttt gagcgcctaa tgtataagtt gcacctgcta
    25621 ggaggccacc agtaagcatc agaccaatac cggtaatcaa aaaatgtttc aatctcaatt
    25681 tatgtgtcct ccttcaaacg cccgctgaat gcagatctct ttttgtaata aaacgtcgtc
    25741 ccatccattt aacaaagttc atcatgacat aaaaaagccc ttttgttcct ttcagcaaca
    25801 gtggcgaaat aaatactgcg cttcctgtgg cgaacagacc gacaccgata aagaataatc
    25861 cactcagaat cgattgtcca aacacactga ttcctgagat gatcatgaat aggccaacac
    25921 ctaagatact gatacataaa acatagatac taaaaatgag cgctagtaaa gcaaccaata
    25981 gtcccccaat aaccaatata aaccccagtg ctagtggaat gaggacagga gaagcaaata
    26041 aaccgataat gattaaccaa atcaggcgaa tttgtctttt tggatttttc actaactcgt
    26101 tttctgcttg ttcttcttct gaaaagtaac tgatttttaa ttttaaggca aactgctttg
    26161 gtgtaccata ttcatcaatg atctgatcta atgtttttcc agaatctaaa aaatattcct
    26221 cataatactg gatcaaatca aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa
    26281 cttcattcaa atattcattc atttgtgctc tcctccaata accggtctaa agaatctcga
    26341 aaaacaatcc attcatcaat aatttcttct aaatgacgac ttcccattgg tgtcaacgag
    26401 taatatcgac ggtttctgcc ttcaaaaggt tcatcatagg tctcaagata tccttctttt
    26461 ttcaatcttc tcaaaaccgg atatagtgta gattcactga cagagagact ttcttgtacg
    26521 atttttgtta aggcataccc atacatatct tccttgctta atgctgctag cacagtacca
    26581 tcaagaagaa cagttggaat ctggatgtgc attacgtcac cttctattct caagtgtaat
    26641 attttatttc aatctatatt atacaacata taatatagtt tcaaaatcag tttgtcaaat
    26701 acaaaaaaac tgactaaaag atttgatcct ttttagtcag caaaaaatgg cttctctttg
    26761 atttttatta taacagtggc ataatccaat ttgtccacat ccagctcagt ggcatgataa
    26821 cgatcattgc aggtatcatg tcagctactg ggaacatttt cacgttgatc attctaaaac
    26881 cggtagcgac catcaagaat ccgccgcaag ctttaaaatc taagatcatg tctggtgtag
    26941 tcaatggata aataaatttt gccaataaga acaaaatata aaaaataatg aattggggaa
    27001 ttgctatcac agaaacgaca taccctaaat tacacgcaaa aatagctgct gtgaaaaaat
    27061 caaggacaga tttagaaatc aaaatcgtac tgtctcctgt catcccagaa tctaaacttc
    27121 catagatccc cgtaccactt gcacaaaata gaacaatgac cgtcactaat gtgctgataa
    27181 attcatcgtg ggtcatacca agcttttcac tagggaagat tttagaaata ggtacttgca
    27241 tcagcgcagc ccccttattt atcccattac ctaaatgaac aatcaaacca atcgctgttc
    27301 ctatcactaa agcaaaaata acagccggca tatttttcat cggcgcaatg gaatagattc
    27361 ccatccccat cgaacacaca ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta
    27421 atttatttcc tactaatccg ccaattagtc cgccaaacaa aacggaacct acattgatca
    27481 aaactccagt tggcatcaaa aaactcctct ctttttctac gaagcgaaaa aaatcagaaa
    27541 aatttctgta attattgctt tcgctgcaag aaagattgta acagacagag aagattacac
    27601 caattcattt tttgtgttaa agtattcttt attggaataa gtaggaggaa tatgatggat
    27661 atccataaat tcgaagtttt tcttgattta gccgaaacaa tgaatttcac caaaactgct
    27721 gatcgacaat tcaccaccca aggaaatatt tctaaacaga ttctttcgtt agaaacagaa
    27781 ttagacgtca aattattcga acgcgcccat agaaaaatcg aattgaccga agctggaagc
    27841 ttattgctcc catatgtaaa aaatgtcgta gaacaatacc atgctatgca agaagtgctt
    27901 gaaacctata cgaaagacaa caatctttca ttaaatatct taacgatacc gacaatgatc
    27961 aattataaag gtttttccaa aatcaccgag tttttgaaac gccatcctga atttacagtc
    28021 cagttgaaag aagtggaaag tgtcgaatta acttacaatg acgccaatga acatagtgac
    28081 acgatccact ttgctagaag tttccaggcc ccaccaagtt cgattgagtg gttgcctact
    28141 gaaacagacg attttgtagc agttctacct aaaaatcatc ctttagctag taaaaagaaa
    28201 ttggatctaa gcgaactgaa aaaggagaac tttttgctcc ttggccccac tacgaacttg
    28261 tatcaacctg tactagatct gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa
    28321 ggagcaagaa tcgacttgat catcaatatg attgcaaatg acatggggat tgctattgtt
    28381 atggaaaaaa cagtcaagaa tctattaaaa gaaaatactg tgattctccc tatttctcca
    28441 acaacagaga gttatcttgc atttactcgt aaagtcggtg agcattcttt ggcttcagat
    28501 acattttggg agtatttaaa tcaagaagaa aactaagccc tacattttct gtccatctat
    28561 ccacccaaaa aaagagagcg acgcatgact ttcgctctct ttcttgcatc atgtccttgt
    28621 ttcaatgaca cctctaacat ttctcgtagt tcaataagaa agcagcgatt tttatgccaa
    28681 aagaaccgta agaacatttc cctaatcaca aaaaaacgac taaaaacccc taaaaacctt
    28741 caatagaatt tttaggggtt ttattctttt tcctattgtt tacttaaccg tatcattgct
    28801 gtcgttccta ctccttcgat actgctataa tcaatacgtc catcatgcag ttctagaatt
    28861 tttttaacca gtgaaagtcc tagcccattc ccagaaattt gacgacttgt atctccttga
    28921 taaaattttt caaacattcg atctctggtc tcttcattca ttcctacacc tgaatcggtc
    28981 acttccagta caatctctgt ggctgtctca aatagtttga tatgaatctg tccattgatt
    29041 tgattgtact ttatggcgtt atccatgata ttcagccaca cttggtacaa aaactcttca
    29101 ttgcctgtgt aatttactgc agcaagttcg atatccaatt ctagctgttc tttctcccat
    29161 ttaggctgaa aaaaaacgat tacttctctg atttgttcat ctaatcgata ctctttcttt
    29221 tccagttgga tacgctggtt ttccagtttg ttcaatttta agatgttctc tgttaattgt
    29281 gaaagctgcg tgatactttc gataatccga tataaaaata tttggcgttc ttcgtcagag
    29341 agatttgggg cttgtaacaa ttgaacatat ccttggattg ttgctagtgg tgttttgaat
    29401 tcatgggata cactggacac aaagtcattc ctcagtgtct cgatactatt gagttcttgg
    29461 accataacgt tgaaatcttt atacagttgc tgtacttctg ctactttctg ctgttcatcc
    29521 attcgaatcg aaaaatctcc cgttgctacc agactcatat ttgtccctag cgcactgatt
    29581 ggatgcagga tacgccgccc aacaaaaatc gatattcctg tacccacgac catactgaac
    29641 cccgcaaaga cgaataacgg gtataacgtt ggttttccag ccgattcagc aggcgagata
    29701 gaatgtcgag acaataagaa aataaaaccg agaaaacaca gcagtgtcac taagatcgaa
    29761 atgaagacaa tactagcaaa atagatccat aattgtgaaa atagcttttt tttcatcttt
    29821 aaataaccgc cttatagcct agtccccgta ctgtgatgat ttgaaaatct tccgtctttt
    29881 caaaacgggt ccttaaacgt ttgatatgta catcgatcgt tcgttcttct gtattcgtat
    29941 ctaatcccca aatatcatcc atcaactgtt ggcgagtgaa gatcttgttg ggataagaca
    30001 gtaatttata cagaagcaga aattcttttt gaggcagaat caacgaatgc tgatcttgta
    30061 acactgtata agcatctagc tctaattttg tttgtccgat tatcaagcta ctgctgtaat
    30121 tgatctttga acgtcgaagc aatgcttcca cacgtaggat catctcattg acatcgatgg
    30181 gttttgtcat ataatcatct gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg
    30241 cctttgccgt aatcattaga acaggtgtgt caaatctggt gctacgtaat gagtctagca
    30301 agtcatagcc atccattttc ggcatcatga tatctgtaat gattaattct atacgtgtcg
    30361 cttctagttt tttcagcgca tcctctccat tcaatgcggt gatgactgaa aagcccgctc
    30421 gttccaacac agaacggtaa agcaactgta attgcggatc atcttctact actaaaatac
    30481 gattcattga tctccctctg ttttttgaaa aatttctaga ttttttcttg tagttcatct
    30541 tcagttaaca tttatttcgt atactacgat cattgttctg atatacttat tagtataccg
    30601 aatatttcaa tacgggagaa gggtgattat gcttcaatta aaagatataa aaaaatatta
    30661 taaagtcggc gaaaccacca ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa
    30721 agaattcgta gcgattttag gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat
    30781 tggtggacta gataattatg attccggtga tatggtaatc aacggaaagt ctactaaaga
    30841 ctttaaggac agtgactggg atgcctaccg aaacaactct atcggtttca tttttcaaag
    30901 ttacaacttg attggtcatc taggcattat tgaaaacgtt gaacttggga tgacattaag
    30961 tggtgtttct aaagacgaaa aacgaaaacg ggcagaagat gcattgcacc gagtcggctt
    31021 gacaaatcat atgcataaaa agccaaatca gttatctggt ggacaaatgc agcgagtggc
    31081 catcgctcga gcactagcca atgacccaga tatccttttg tgcgatgaac caaccggagc
    31141 gttagataca gaaacaagta tccagatcat ggaattgatc caagagttat cgaaagaaaa
    31201 attagtgatc atggtcacac ataatcctga actggcccat caatacgcag atcgaatcat
    31261 tgaattttct gatggtaaga tcttaacaga ttcccatcct cacatcgaac gaccaaaaga
    31321 tgatcaattt aacttacgca gaacaaagat gagtttttgg acagcgttaa agctttcctt
    31381 caacaatatc cgaacaaaaa aaggacgtac tttcttgacc tcctttgctt ccagtattgg
    31441 gatcatcgga atcgcgatcg ttctgtccct ttcttctgga ttccaaaaac aaatcgataa
    31501 cacgcaagct gaaacgatgg cgaaatttcc gattactatt tctaaggtaa cgactaacca
    31561 gactcgtgac gatgccggat tgggtgcaag caaagcggat tatccagaca gcaaaacgat
    31621 tactgcaaaa gtcagcgaag aagataaagc acagcacacg aataagattg atcaaacata
    31681 cgtcgattac gtaacagaca tcgatcctaa cttaagtaat aatatcggat ttacccgtac
    31741 tacaggaatc aatcttttac gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa
    31801 tcaaaatcct gatacggaat ctctttcttt atctagtacg atgtcagcaa tgacaggtgt
    31861 gggagtgtca agtttcccta cacagcttga cactagcaaa gaaaactttt taaaagacaa
    31921 ttattcttta cttgccggaa gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga
    31981 cggcaataat aatactaata tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga
    32041 cgaaaaactt gattttgatg atatcgttgg tacaacattc aaattagtaa acaacaatac
    32101 ttactataca aaacttccaa ctgggaattt tatccctaat acagattatg atgcgatgta
    32161 tcagaatgct tctgatgaat tgaagatctc ggggatccta cgcgtgaaat cttcttctac
    32221 aatgaatctc ttatctccag gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa
    32281 cgaaaacaaa gaatcagaaa tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac
    32341 tactgaaaaa gttgacgaaa atgccaaaca aacattgctt agttatttag gtggagacag
    32401 cttgccttca agtatcatga tttatccgaa caattttgaa gacaaagaaa agatcttgga
    32461 ttatctagat gattacaata aaggcaaatc agatgaagat aaaatcattt atactgattt
    32521 agctggaacg atgactgaac tgactggcgg attgatggat gctatcactt atgtccttat
    32581 tgcttttgca gggatctctt tagtaacaag tatgatcatg atcagtatca ttacttatac
    32641 atctgtgatt gaacggacaa aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa
    32701 agacatcact cgcgtatttg acgcagaaac gtgtatttta ggtatttctt ctgggatatt
    32761 aggtgtgttt attgcatggc tagctacgtt ccctatcaat agcatcttat acaacatgac
    32821 ggatctaaaa aatgttgctc aactaaatcc tgtccatgcg attattctag tgatcgtctc
    32881 tactatattg acgatgcttg gcggtcatct tcctgcacga atggcagcga aaaaagacgc
    32941 cgctatcgct ttgcgtgcag aataaggttg tgacaaccac atttagattt gagtcataag
    33001 agcgctgaaa tagtagaaaa cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc
    33061 tgtttttttt aattattttt tataattctg gtatactttt ctttcctttg taccacaact
    33121 cttctggcgt atacctgtct tttccattta tgaatactgt tccatctgct tcaatcgtta
    33181 cttcagtccc aatccacata ttacgagaaa gtgcgagaag attttttctt tttcctcttc
    33241 ctttttttcc aaattgcaag gcatcggtta tctcttctgt ccaatgagat ttcacgtatt
    33301 ttacttctcc gttttcaaat gtaactttca aaatattcgc tatattttcc acttttactg
    33361 ctttaggaaa tgactcctcc atttttttca ctttccttcc tccaacacta agtatgcgct
    33421 ttcttttagt gataagtata tcatagttta ttctattatc aaaaaaacgc atccattcca
    33481 ttgtagaata ctttaccgaa caagttgaat tgttttcctc tttttcacct tttactatag
    33541 ataggtaaag acaactaagg aggaatctct catgacagat caaccgtatg atttaagaaa
    33601 agtcatcgaa gaactaaaaa cacttccagg tcaatatcac gaaacagata ttgaaatcga
    33661 tcctgaagca gaattatctg gtgtttaccg ttatatcggt gctggcggta cagtaaaacg
    33721 tccgactcaa gaaggaccaa ctatgacttt caacaatatc aaaggtttcc ccggcacacg
    33781 tgtgaacatc ggaacaatgg ctagtcgtaa acgtgtaggt gaaattttgc accatgatta
    33841 tcgaacactt ggtcgtttct tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt
    33901 agacaaagca gacgctccag cacaagaagt cgttcatcta gctaccgatg cagacttcga
    33961 tattcgcaac attttgcctg cacctacaaa tactcctgaa gatgctggtc catacatcac
    34021 aatgggtgta gtcttaggat cagatccaga aaaaacaatg agcgatgtaa cgatccatcg
    34081 tatggttcta gaagataaag atacgatcgg tatgtacatc atgcctggcg gacgccacat
    34141 tggtcacttc caaaaacaat tcgaagcctt gaataaacca atgcctatca ctatcaatat
    34201 tggtttagat ccagcaattg ccattggcac gactttcgaa ccacctacca ctccacttgg
    34261 ctacaatgaa ctatggtgtg caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg
    34321 tatagcagtc gatgaagtgg gtattgctcg tgctgaattc atcattgaag cagaaatcat
    34381 gccaaatgaa acaatgcaag aagatatcaa cacgaactca ggaaaagcaa tgccagaatt
    34441 tcctggctat actggggatg ctaatccagc tgtacaagta gtgaaagtca aagcagttac
    34501 tcaccgaaaa gacaatccaa tcatgcaaac aacgattggt cccagtgaag aacacgtatc
    34561 tatggccggt attcctacag aagcaagtat tcttgccttg gtcgataaag ctattccagg
    34621 aaaagtagta aatgtctaca atcctcctgc aggtggtggt aaattgatga ccatcatgca
    34681 aatccgtaaa gaaaacgaag cggatgaagg tattcagcgt caagcagcta ttcttgcttt
    34741 atcagccttc aaagaattga agacagtcat tttagtcgat gaagatgttg atattttcga
    34801 catgaacgat gtagtttgga ctttcaacac tcgtttccaa ggagataaag atatcatcgt
    34861 gatcccagga atgaggaacc atccattgga cccatcagaa ttaccagaat ataatcctgg
    34921 ctctattcgt ttccgtggga taagtgcaaa aactatcttt gacggtacag ttcctttcga
    34981 tatgaaagaa cattttgtac gtgcaaaatt ccaagaagta gaagattggg aaaaatattt
    35041 gaaataatga atgatctgca actgttttga catcataaaa aggagtgcgg aaaatggaac
    35101 aattggcaca acggaagaaa aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt
    35161 atgccatcaa cctagtgaag aaactaaaag aatatccgat gatagaaaca cacggaatca
    35221 tcagtccatg ggcaaaacag aacttaaagc tggaatccga cttatcacta actgaattcg
    35281 aaagcctgtt tgattaccat tattcaaaca aagatttagg tgctgctgtc gccagcggat
    35341 catttttgac cgatggaatg atcatcgtac cggcaagtat gaaaacagtc gcaggtatct
    35401 ctgttggttt aggcgacaac ctgattgcac gtgctgctga tgtaacaatg aaggagcaac
    35461 gaaagttagt cattgttcct cgtgaaacgc cattaaatgc cattcacttg gaaaacttga
    35521 ctaaattagc acgactaggt gtgcaaatca taccaccaat tcctgctttt tataatcagc
    35581 ctcaaacgat ccaagatctt gttaatcacc atacaatgaa acttttggat caatttgaaa
    35641 ttgtaaatga gcattcaaaa cgttggcagg gtgattaata tggaaaaaga aacgatagtt
    35701 gaagtaacca aactaggttt cacgatccaa gctgctgtta aagtaatcgg taaagactta
    35761 ttgatcactt taacaggcgg agatacacca catatcggaa cagtcactac attttctcgt
    35821 gatacagaga tgcaaactgt acgtttccct agccacgatg gtcggttcca taaagacgat
    35881 gtcttagccg aaaaaatagc tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc
    35941 acttccggta tccatgttga ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg
    36001 tcagaagagt taggttacaa gattttagat tggttggaaa aaatagattt ccatatcaaa
    36061 gaaccagttt attataaaga tggtgaaaaa ccattatgaa agaagaggtt gtgaaatcag
    36121 cgttatgacc tgagtatgag agaaaaaaat ggaaaaatag cttctcatat tttgccgttt
    36181 ttgagctaat accgagggac agctgattga acgccgttta tcaagaggtt gtgataaagc
    36241 cgcttcgttt cgagtagtaa gaaaaatttt gtaaacatgg gtttccacat ttttgcataa
    36301 atttgactta ctaccgagaa actggctttt gaacaccgtt tatcaagagg ttgtgacacg
    36361 tcttttgtca caacctcttt tatttttgct tcactgtaac tgctacaata tcaccgattt
    36421 cttttccgat gtttttacga atatcttttc gtagtccaag gatgtagcag actgaaccat
    36481 ctggattttt cacgcccata ttcacgatac tgccttcata tggcacattg tcaaaagttg
    36541 cttggacttt aactcttcca gcaccgaatt cttttcttat atcatacgga aaaataatat
    36601 atgccccgcc cttgccgact tcggaagcat agatttttga ttcaaaagaa taaatttccc
    36661 tcattttatt tctcctattt tttacatcta gtatagcata gcaaaaaaaa gaaggaactg
    36721 tgacaaaagt cttgtcgcag ttcctttttt cgaataaacg gtgttctcct gccaactcct
    36781 cggtcacaag tcacaatagt caacaaacat gagaagctgt tttttgaaat tgctccttgt
    36841 gactcagagt taaacggcag gctcacaacc tcttccttaa acggtgttca aaagcttgca
    36901 tctacggcac taagcccaat cagatgaaaa atatggagtg ctatttttat ctgattgttc
    36961 ttactgcttg atgcagagca gctttttcac aacctcctac atggtgttca aaatattatt
    37021 cgccttttgc ttctttcact aattttgcga atgcttgttg ttggaattca gcagctaagg
    37081 ctttcgtacg ttgttcttgc tctgctttgc tcatttttgg attttcagtc attgctttta
    37141 ccataccgtc ttgaacataa gcttttgctt cactttccat cgtactttgc tgtttcttca
    37201 ctaattcagc taacttggct gcttggtccg tacttaattc tacccatgtc tctgggattt
    37261 tgaatgtatt cactcggctg tcatattcgt gattgttatc agcaatattg aaccagaatt
    37321 tatacatgtc gtttttgtat tcccaataag tagttttcgt cactttttca gcatcgccgt
    37381 ctgtcttttc tactttattt gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt
    37441 tttcatttgt tcggtaaaga tacacttttt cttttccatt ccctaaagct ttatacaaaa
    37501 gcatatctgc tccttgggaa tcagcactgg aaacaatttt ttctgttttt gtttccgtta
    37561 ctttttccat tccaaagtga ttggacaaat tagcgacgat caaacttagt gaagcgacga
    37621 agattagtcc gaaaatcaaa gataaaaagg tttgccatgt ttttttagcg aacacgttcg
    37681 ttaaagcaaa agcaagcaag ctgacaatca aaactccaat aatcatgcat tatcccccgc
    37741 tttcattaga acaggttctt ttttcttcaa taaaaaggca ataataaagc caagaatccc
    37801 aaagatgatc gctacgataa acgcggcatg gtaacctgac agtgtggcat tgattgcctg
    37861 atctttataa gctaatggcg tattatccaa tacagatttg cttgggatat ttgcttttgt
    37921 cacattcgtc aacacactga tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa
    37981 tgtattattt accgctgtcc catgactgat caagttattt ggcaatgcat tcataccaga
    38041 agtcgttacc ggcatcatga ccatggagat cccaaacata cggatcgcat aaaggatcac
    38101 gatatataaa actggagtcg tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa
    38161 aatgaacatc cctgtgatgg ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt
    38221 cacaggcatc atagccccca tgatcaatgc acccggcaac aacatcaagc cagaatggaa
    38281 tgctgattct ccacgaatat tttgaatata caatggtacg accatttctg cccctaccat
    38341 cgccatattt gttacaccag ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac
    38401 acgaagttcc aagaatggat gttccaaatg taattgacgc catgagaaca aggcgatgaa
    38461 aatcacacca acaactaaga aaccaattac tttcaagctt ccccagccgt catctcctac
    38521 actagaaaat ccgtaaagta aactaccgaa tccaatcgtt gataaaatag cggataaaat
    38581 atcaatgcta ggatttgaca atggaataac acttttcatt aggaagaatg ctaaaaccaa
    38641 gacaagaaca acgattggca gtaccatacc aaacaagtca cgccaatggt agttatcaat
    38701 gatataacca gataatgtag ggcctaaagc aggagcaagt ccaataacga tccctgacat
    38761 ccccattgct gatcctcttt tttctggcgg gaaaatagat agcatgatat tttgcaataa
    38821 tggcatcgat actcccacac cgcaagcttg gatcaaacga ccgattagta atgtactaaa
    38881 attaggtgct gtaaagcaag taatcgttcc gatcaaaaag atcgtcatgg cagaaagata
    38941 taaatttctt gaactgaatt tattgatcag ccatgctgtt attgggatca tgatcccgtt
    39001 gactagcaag aaaccagtcg ttaaccattg cacatcagac gctgaaatat cgaaagcttt
    39061 catcaatgta ggaaaagctg tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc
    39121 aattaataag acaacaacta aaagtgtccg attatatggt tttccgtaaa tatctacggg
    39181 ttggttgtta ctcatatttc tccttctttc tgaatttttt ctgaaactac tagaatactt
    39241 tagtcctaaa ttcgctattt gtcaacctac ttgacacata agttaatacg gatataatta
    39301 agggaagcaa gaggacaaaa ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt
    39361 tggaaaatga tcaattggtt gttggcatca gtgaattaag tgatatcgta ggtgtgtctc
    39421 cacgtcaatt aaggtattgg gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga
    39481 attgtcctag aaaatatcgt ttacctacag tcataaaagt agaactgatc aaaaaatatt
    39541 tagatgacgg atttactttg tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa
    39601 tgcaccatgt ccgtaaagtt ttttcaaaag caatcaaaga tatcgaattg atcgacgaac
    39661 gctatacgat tttacaaatc ggtaacttca aagaagagaa tgctaagatg tacatcattc
    39721 atgacaacga gacagacgaa ttaacttaca agctccttcc tgtcgatgaa aaagctgact
    39781 acgaacagtt gttaaaagaa ctctaaacgc cactaatctc ctaagtacca agctttttaa
    39841 taagtagatg ctacataaat acttgaaaaa aggcctgttt gaacataaga atatccgaac
    39901 ggtgatctgc gtttctttca tatagaaagg aattctccat cactgttcgg atattttttt
    39961 atttttttcg gttactttct tctaatacat ctgttagcca atttgcatat tctgtttccc
    40021 gattgatcgc atgcgctaaa atcaggtagt gtccgtaatt ttcatgtttt tctttttcag
    40081 taggaaagat cgtttttttt cgctctaata gatgaagtcg tttttcttca tgaaggtcac
    40141 gctgctgctg aataatttct actaatgccg gatcattcac gtctttgacg aaatacaatt
    40201 tcagaacaaa ttcgtctcga ttaaccggta gttcattggt gggtgtatga atccactctg
    40261 ccaattgttc cttcccttct tctgttaatt cataaacttt cttttccaat ttttcacctg
    40321 tgatctctac ttgatgctga atgattcctt gctcttccat ttttgcaagt tcaggataaa
    40381 tctgactatg ttttgcttgc caaaattctc ctatttcatt tttgaacgat tgattcaatt
    40441 catatcctgt catcttcttc ttacttaata accctaacaa aatatatcga agtgtatttt
    40501 ttcttgccat cagttttccc tccttgacaa atctttaaat ccattctatc ataaatgagt
    40561 aatcgaaaac atgtagttta ttacatgttt taatttataa ggagatgctt aaacatgaaa
    40621 gaatttaaag aattggaaga ttttattgga actcacttta tctatacgta tgacaatggt
    40681 tgggaatatg aatggtatgc caaaaatgac catactgttg attaccgtat ccatggcgga
    40741 atggtcaaag gccgttgggt aaaagaccaa gaagctgata tcgttaaatt gacggacggc
    40801 gtattcaaaa tcacttggac agagccaaca gggacagacg tggcattgga ctttatgcca
    40861 aatgaaaaga aacttcacgg aatgatcttt ttccctaaat gggttgaaga acatccagaa
    40921 atcactgtta ctttccaaaa cgaacatatc gcagaaatgg aagcagctcg cgaaaaatat
    40981 gaaacttatc caaaattact tgttcctgaa tttgctacga tcacttatat gggtgatgca
    41041 ggtatagaca atgaagatgt tattagcgaa gaaccatacg atggaatgcc agatgatatc
    41101 cgtagcggaa aatacttcga tgaaaattat aaacgagtaa aataaggttg tgaaagagag
    41161 gttgtgaaat cagcgttctg acctgagtat tagagaaaga aacggaaaaa tagttcctca
    41221 tgttttgccg ttttttgagc taataccgag ggtcagctga ttgaacaccg tttatcaaga
    41281 ggttgtgaaa tcagcgttat gctttgagta ataagaacgc tagttagaaa atagcttttc
    41341 atattttcta ggctagtggg cttattaccg agaagcagcc gtttgaacac cgtttatcaa
    41401 gaggttgtga aatcagcgtt ttgacctgag tattagagaa acaaatggaa aaatagttcc
    41461 tcatattttg ccgttttttg agctaatacc gagggtcagc tgattgaaca ccgtttatca
    41521 aagattgtaa caactgcata ttcacacagt taaattagtg acttcaaaat gaaataatgc
    41581 tcccagcaaa aaattaggct gggagcatta tttcgttatt ctgttcatca aaaatcagcg
    41641 ttttgattcc atttcatcac aagttcctcc tcatttactg cttcatcttt ttgctgtgtt
    41701 tgaattgtaa aaccttgtga aagatagaaa tgaatagctg attgattttt ttgatagact
    41761 gtcaaagtca gtgttttatt ggtttctttt atttttttca ttagttgcgt accgattctt
    41821 tgttgccggt attccctttt cacaaagatt cctgcgataa actgatcaat caccccagca
    41881 aaaccaataa tttcttgatc tatttctgct aagtaaatca ctgcatctgg taaagctttc
    41941 ctaacagatt ctacttgatt ctcccaataa gttgatggaa tgaattcatg agaatccaag
    42001 ttagcatgca gccaaatcga tagaacagat tccagttctt tggcagacaa ttttgttgtt
    42061 gtgcggatca ttttttctct ccttacttat tcattgattt taaaataaaa accatttttt
    42121 tgaagctctt ttacaagttt tcgcccctca aaaaagtata tcttctcttg ttttactttt
    42181 ctaataccaa aagaaacagc aactttagga tcatcagctg aaacgattcg ataagttcca
    42241 ccaggcaatt gatccagctt gactttccca tgatggtcgc tttgtactga aaacggcaca
    42301 ccatttctag taagtgtttt tttcttatgt ttcaaaatga atgaactacc atttaccggt
    42361 ttccctgacg aatcactaat aataaatgaa aaatcaagca cttttttact taatctatac
    42421 tgatagatta gtcgtccgat aaacactaac agtatgacta cagctattat cgttcctgca
    42481 agaataaacc attgccgcat ggttctttcc tgatttcctt ttccttcttc tttttttacc
    42541 gattcgttat aaggaatccg atgtcctgtc actagcaaac gatgcgtgtt gatcatatac
    42601 ggcgtacatg taagcaaggt aactaagtct tctccttctg aaatcgttag aacagacgtg
    42661 tcatctggca gtaccacttg tatattttta acttgataag ccaatttctc ccccaaaaca
    42721 gtcaaaataa acgtgtctcc gttttctaaa cgatctaatt cggtaaataa ccgtcgatta
    42781 ggtaaaccgc tgtgagcaga gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag
    42841 gttccttgca agacgcctgc tccattttca aggatcctac cattgagccg atcataaagt
    42901 gaaatattta attggatctt aggtatattg atacttccga tcagccattc ctctaattgg
    42961 ctggatgttg tactttcatt tctgttcttt tcattgaaag gatcagtacc aggattgaat
    43021 ccttggtcag ttaatcgttg gtttttctcc tccattttct ttcgctgcac ttcaatttca
    43081 gctgcggtcc gtttttgtac ttgttcgata cgtttttgat caataaaact atttaaagaa
    43141 tccacataaa atggatataa agcgatcagt aaccctacga cgatacatat taataaaaac
    43201 aaacgaaaca aaagtcgacg agactgtttt tgcttactct gcttcacatg tgtcctccct
    43261 tgcaagtttt tttgttcttt cttttattat atgggttcag gaaaaaaata gaaaatgaaa
    43321 gccgatgagg ttgtgaaaaa gctgtcctgc atcaagtaat aagtacagcc caaaaaaata
    43381 gctactcata ttttttgttg ggttgggctt attaccgcag atgcaagctt ttgaacaccg
    43441 tttaaggaag aggttgtgaa aactgcgtta tgctttgagt aataagagcg ctagtcagaa
    43501 aatagcttct catattttct aggctagtga gcttattacc gagaagcagc tgattgaaca
    43561 ccgtttaccg agaggtcatg aaaaagctac tttgtttcga gtattaagaa aaattctgtt
    43621 tcaaaacaag gagatgcaac aaaacttatg tcgcatcctc tctccttaga actttcactt
    43681 atgtttttct tgtaaaattc ataaatattt tcatgaattt tctaatttct tgcctacctc
    43741 tcgcatagga tatatgatga atttatctaa agtttgcaaa ggagttcgac aattgaaaaa
    43801 atatggccca tttttagcaa tttttttcat tgctttggca tttctcatgc ctcatttatt
    43861 cacacataaa ctaattttgg gtgctgatgc cccattccat tacaatcggt tctatgatgc
    43921 cgcgcaacaa attaaatatg gggatttcca ttattttcct tccctttatg gattccagca
    43981 aagtggcagg atcgtgaatg ccgtttacgg cccatttttc gcttatttac aaggattgat
    44041 cgtgttgctt gcaggtagct ggtttcatta tcagattctt tcaaatttca ttctttatct
    44101 tatttcagga ttttctatgc tgattttact gagatatgtc aatgttcgaa attgggtaag
    44161 tgtaccagta gccgttttat tcatgagtac gtattctatt caatactggt ctatcaacca
    44221 aggattcact agctggggga cttgttttta tcctttatgt atgatcccta ttgctgattt
    44281 cgtccttcat aaaaagttcc ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca
    44341 gatccattta ctgtcttcga tcatgttagc tttgatgtat atcccttttt atctttatta
    44401 cttttttaac cagactgaaa aaggaaaagc actgcttcaa ctgattaagt cggtcttgct
    44461 ttattttgtt ctgacagcaa atatttgggc aggattagct attgtttaca gtgggaataa
    44521 gattcttcct ccctttgtta acagagaaat gagtcaaaag gcgatagatc tggatggcag
    44581 ttattggctc cattacccta aggcctttcc tatattgata ttatcaggtt ttttgctttt
    44641 tattttcttt caacaaaaga acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt
    44701 tttctttttt ctttctacta gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc
    44761 ttttattagt ttgatccagt ttccatttcg atttttcgtg ccatttacgc tattgcttct
    44821 actttacctt gccctttcac tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag
    44881 tatatttggc attcttattt tttcagcaca gacgatccag aacttatacc agcatctgga
    44941 aaagtgggaa aatgaaacat ttgtcagtcg acatacttat ctattcgata cgccggaaga
    45001 agcaaggaaa acattcttca gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac
    45061 cacacctgac tatctgccaa tctataagga gacaaaagac aataaatacg accggtataa
    45121 cgaagaaata ctagaaaacg aaacaacttt tataaaaaca catccaggcg gtcttttgac
    45181 aatcaagtgg aaaaacactg gggacaaaac tgttcatatc cctgttatcg tctacgatcg
    45241 gactgtgcta cagcaaaatg gaaaaacatt gactgattat gaagtaacag atatcgggac
    45301 cccaatcgtg aagcagcaaa aaggaatcaa cgagctcacg cttcattatc aaacgccaat
    45361 ctatttctat ttcattcttt ctcttacttt gattggctgg tttaccttgc tatgtttatt
    45421 tatttatcat agatacaagc ttttacgtgc ttaggcaaaa gggataaata catccgatca
    45481 aaaaactctc caaacggtat aatcgtttgg agagttttat tttaatcagt ttttttatgt
    45541 tatatgttat attttttatt cgttcacatc taattcgatt tccaccccgt agtgatcact
    45601 gatgattgga ctatcgatcc catcaaaaat ccgttgatat tttttaacct gcattccttc
    45661 agggacgaaa ataaaatcta tacgcaattt ttctgtattt tgttcccaac catctatttt
    45721 cttttccaca gtagctgctt ttcctttttc attcgcgaca acatacgaat cttgaagaag
    45781 aaggcaactt tctcttacag tataataacc ttgagagtct actggattat tgaaatcacc
    45841 taggaaaaaa agtggtacgc gagtttccaa aaagtagttt tccaagtttt tccactcaaa
    45901 ataaaagcca ctttttttat ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc
    45961 ctgatcatct atctttgttt tcccaatcaa gatcttgcgt gtctgataat cttctttctt
    46021 ttgaaattgg gagacggtaa ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt
    46081 tccttcttca tatttatcat aaccgatgtg gctcatttcc caactccaaa aatactcttt
    46141 tccgtgttcc tttaaaagct gaactaaacg gtaggcaaaa ttatctttac ggataggtac
    46201 ttgattcttt accggacaga acttcattag cttcgcaggt tctacttctt cagattccag
    46261 taattggttg atttcttgta aagcaatcac ttcataatct tctaacagga tcttatctgc
    46321 taattcttgc aattttttct ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat
    46381 gaatttcatt ggttttctcc ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt
    46441 tgtcacaacc tctccagcaa atagtgttct cctgctaact cctcagtaat aagctcaatg
    46501 gaccaggagc tattttttgt ttggctgttt ttatcacttg gtgcagggca gctttttcac
    46561 gacctctcca ctaaacagta ttcaaactgc tgcttctcgg taataagccc aaccaaacga
    46621 aaaataggag gagctatttt tgtttggctg ttcttatgac ttgatgcagg acagcttttt
    46681 cacaacctct ccttacattt taatggagcc gatcacttta ccagctgtca ctgtacctaa
    46741 ttgcttgatc actacttctt ctactttatc gtttgtaaat acgatcatga tcgttggatc
    46801 tttttcttcg ttttggatca catcaagatc catctcagct aatttcgttc ctgttcctac
    46861 ttcttggcct tctttgacag caatctcaaa agctggctga ttcatttgga ctgtatcgat
    46921 tcccatatgg atcaaaactt cgataccatc tttcgtttcc agcccaattg catgtttgct
    46981 agggaaaata cttgtcactt ttccagcgat cggcgcaaca acttctcttg atgttggaat
    47041 aacagcaaag ccgtcaccca tcattttttg tgagaatacc ggatctttta cttcagataa
    47101 aggaatcact tctccagaag ctgcggaaac tagttcttgt tctataccta agtattgctt
    47161 actacctgct tgtccatttc ctgtagaaac catttctggc tctggaatct ctgctcctga
    47221 ttgcagcaga tcttcgatat ctgatttcaa gacatctgct tttggtccat aaactgcttg
    47281 gactccattg tctttgacga tcaatcccat cgcccctgct cgtttccagg cttcttcagt
    47341 ccctactttt tctcgatcat ttacacttac gcgtaaccga gtcatacaag catcgacatc
    47401 tttgatattt tcttttcctc ccagcaaatg aatgatctgg accacttgct gatctgctgt
    47461 tcctgttccg ctgttagata caacggtttc ttcactgtca ttgtcgtaat ttccgttacg
    47521 tcccggtgta gcaaagttga attttttgat caagaaattt gctaagaaac acgtaacaac
    47581 accaaaagcg atcacacaca acacgaagtt gataagatct ccacctagac ccgctttgat
    47641 agctaacggt gtccgagtca gcaattcgat attcccaaat gaatggaccc gcaatggcaa
    47701 gatatctgcc atcgcaaatg cagccccttg gatcactgaa taaatgacat ataatggcac
    47761 agcagcaaac ataaacatga actccaatgg ttctgttaca cctgttagaa agacagccaa
    47821 cgctgctgaa aaatacattg atttatatct tgctttctta tcaggatcga cattacgata
    47881 catcgcaaaa gccattccca tcaagatacc tgaagaacca atcatttgtc ctactttgaa
    47941 acgagccggc gtccagttat gcaacacaaa ctcatattgc gacatatccc ctgcaccttt
    48001 aagattcact aaatcagtgg cccaagccaa ccataacgga tcttgcccaa atacttgtgt
    48061 ccccgcctgt gcgcctgata aaatctcata cgatccgcca agctgtgtat agttgatagg
    48121 aatcgtcagc atatgatgca atccaaatgg caatagtaaa cgttctaatg ttccaaataa
    48181 aaacggtgct agaatcggtg cgctttcttg tgattctgca atccataaac caaaattatt
    48241 gattccagcc tgtacgtttg gccacaccac agcaagaatc aatgccacga ttgttgacca
    48301 taagataaca acaaacggaa caaagcgctt tccattgaaa aatgacaaag catccggcaa
    48361 ttttctataa ttatagtact tattataagc cattgccccg acaaatccag cgatgatccc
    48421 tacgaatact cccatattca aagctggtgc ttccaataca cttgtaaaaa atcctttaac
    48481 catgatcttc gtaccgaaca gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact
    48541 tgtgacacca aagatagccc cagtgatccg attgattaaa ataaatgaga tcccagctgc
    48601 aaatgctccg cctgctcttt cttttgccca acttcctcct attgctaaag caaataaaat
    48661 atgaagatta ccaataattg cccaaccaat attttcaatt actccgcccg ttgtgacaag
    48721 cggcgtccaa tcaggattga tcaagggaat tgatttccct atactgatca tcaacccagc
    48781 tgccggcata actgctacca caaccattaa agcctttcca aatttttgcc aaaactcaaa
    48841 actaaatatc tttttcattc cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt
    48901 atagcgtgcg ttttcttttt tgtaaatgct ttatttttta tttttctgtc tataccattc
    48961 tataaaacct ataatacgtg tgttcccttc tataattttc tcctaaaaat ttattttttt
    49021 aagctttttt ctatgaaata ttttcttttg tctattgact tgtgcaatcg gttgcgttta
    49081 ttatgaacgt ataaaacagc tcattaggag gagaaaaatg aaacagatca aacgactatt
    49141 tgctattgat ccatggaaaa tcagcacgac acaattagat aaaaaaaatc ttcgactgca
    49201 agaatcttta acaagtattg gaaacggtta tatggggatg cgcgggaact ttgaagaaac
    49261 ttactcaggg gaccaccatc aaggtactta tttggcgggt gtctggtatc ccgataagac
    49321 acgtgtcggc tggtggaaaa atggttatcc agaatacttt ggaaaagtga tcaacgcctt
    49381 gaactttatc gcgatagata tttcgatcaa cgatcagctg atcgacttgg cttctttgga
    49441 accagaagac ttttactggg aattagatat gaaaaatggt gtattatctc gtacattcac
    49501 aatcactact tctactaata aagtacgcat ttccttcgaa cgttttctga gcattgtcaa
    49561 aaaagaatca gcttatatct atttaactat agaaatgctt gaaggtagcg gaacagtcaa
    49621 actcgtatca aaattggatg gaaatgttca aaacgaagac agcaactatg atgaacattt
    49681 ttggaatgag attgatcgtg gaactataga agaaatcggc tatttgacga ctaaaactat
    49741 tccaaacaac tttgggattg aacaatttac tgttactgca gcaatgcgcc atttcgttaa
    49801 tggaaaatca gtcatccctg actatcacga agatacttta gcattacatg gttgcttgtc
    49861 tgcagaatta tctcaaggtg agacatggtc agtggaaaaa gaaattatcg ttcttactag
    49921 tagagatatt ccagaagcgc agcaagcaga aaaagctgtc tcagaattaa cgaaccagac
    49981 aaacaattat atccaagcaa aagaagagca aacagcggct tgggcagaac gatggaagct
    50041 tgccgatgtg gtcatcgaag gagacgaaga agcacaacaa ggtattcgat tcaatttgtt
    50101 ccaactattc tctacttact acggcgaaga tgaacgattg aatatcggcc ccaaaggatt
    50161 cacaggagaa aaatacggcg gagccactta ctgggataca gaagcgtatg ctgtccctct
    50221 ttatcttgct ttagccaaac ctgaagtaac taagaatctg ctgaaatatc ggcataatca
    50281 actgccgcaa gcaatccaca acgctcagca acaaggatta aaaggcgcgc tttatcctat
    50341 ggtcaccttc actggtgtcg aatgccataa cgagtgggaa atcacattcg aggagattca
    50401 ccgaaatggc gcgattgcct atgcaatcta caattatacc aattacaccg gcgatacgga
    50461 atatttgaaa aatgaaggac tggaagtttt agcagaaatc gcacgcttct gggcagaccg
    50521 tgtccatttt tcaaaacgag caaataaata catgatccac ggtgtgaccg gcccaaacga
    50581 atatgagaac aatatcaata acaattggta tacaaatacg atcgcagctt gggtcttgcg
    50641 ttacacgcga gaaaattatt taacttatca aaaagaagct gctatcacta tcagcgaaga
    50701 agaactagcc aaatggaaag atatcgcaga gaacatgtac ttcccagttg acgaagaatt
    50761 aggaatcttt gtccagcatg acacattctt agataaagat ctcatgcctg tatccgatat
    50821 tcccacaagc gatcttcctc tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg
    50881 cttcatcaag caggcagatg tactacaggg gatttatttc ttcaatgata cttttacaaa
    50941 agaagaaaag caaaaaaact ttgatttcta tgaaccaatg actgtccatg agtcttccct
    51001 ttcgccaagt atccattcga ttttagcagc agaattagga atggaaagca aagctgtaga
    51061 aatgtaccag cgaacagctc gcttggattt ggataattac aataacgaca cagaagacgg
    51121 cttgcacatc acttccatga ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat
    51181 gaaaactgcc catgatcagt tgagattcgc accatttctg ccaaaaactt ggaaagctta
    51241 ttctttccat atcaattatc gcggacgttt attgttcatc tcagtcacag atgaacaagt
    51301 tactttcgag ttattagaag gttctcctct agaaatttac gtctatgaac agtcttattc
    51361 tttagcggat cgtctagaaa taacgctaag aaaggaggaa gcaaatgttt aaaggggtat
    51421 tgtttgattt agacggtgtc attaccgata ctgctgaatt ccattatcgt gcttggcgaa
    51481 agcttggtca ggaaatcggc attacgatcg accacacatt caacgaacag ctaaaaggcg
    51541 tcagccgaga agactcactc tctttgcttt tagcttatgg cggaaaagaa cactcttttt
    51601 caaaggaaga atttgcagaa ttagcgaaaa gaaaaaacga ttattattta gagatgatcc
    51661 agacgatcga accaaaagat gtcttccccg gggtcgtgcc tttactggat tctttaaaag
    51721 aagaaaaaat caagattgct cttgcttctg caagtaaaaa cgggccattc ctacttgaaa
    51781 aaatgggtct tacaccttac tttgatgcta ttgccgaccc ggcagaagca acaaacggaa
    51841 aacctgcgcc agatatcttc ctccttgcag caaaagctgt cggactaaaa gcagaagaat
    51901 gtatcgggat cgaagacgcg caagccggta tccaagccat cctctcaagt ggcgctcaac
    51961 caattggcgt gggacgtgct gaagaccttg gagaggagat cccgcttgtc cctgatacga
    52021 cttttcttac gatcgattac ttgaaagaaa agtggcatga ccatggataa tccagagatc
    52081 tccgtcgtac cagttccaga gactgaaata aagaaaatca cattgaagaa cgactactta
    52141 gaagtcgttc ttctcaatta cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa
    52201 ggaaaatcag aaaatgtttt attgtcttac gatcgtttcg aagatgttct aacagacaaa
    52261 agcttttttg gagcaactgt cggacctgta gccggaagga tccgggatgc ttcttggggt
    52321 gagcatagat tggagaaaaa ctgcggatca caccatatcc acggtggcac gaaaggctgg
    52381 tctttccaat tttgggaagt agaagttttc aaaactccat catcaatagg tgtggtcttt
    52441 tacttaaaag atgaattttc tacctatcca ggaccaatca ctgctacgat cacttaccgt
    52501 ttaacaaaaa acgaattaga gatgacaacc tctgctagta gccttgttga aaccatctgc
    52561 aatccgacca accatgccta cttcaacctt tcaggaaatg gaacaagaga catctatgaa
    52621 catcagttaa cggtcttttt agatggtgtg cttgaattag atcaagaaaa actgcctact
    52681 ggaaattgga caaaaaaaga aaaactgcca atcgacttta gaaaatcccc aacactgcaa
    52741 gagatccttg catgttatcc agatggacta gatgatgtat ttcttttgca tcatcctagg
    52801 ctttccaaaa cttccttaca attattcgag aagcattctg gtcgccaaat gactatagca
    52861 acttctaata aaagtatggt attattttct acgactggat tcgaagctga tttctctgtc
    52921 aatggaaaac agatgcattc aaactacgga ttagcaatcg aaccgcaaga aatccctgat
    52981 atcgttcatt tccccaaatt tggatcgatc aacctccatc ctggtcaaga acggatcagt
    53041 cagacgattt atcggttctc tgcacaataa caagagaaag gtgtttttat catggctatt
    53101 accgtaaaag atgtggcgaa aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc
    53161 aacgatcatc ctagtatttc cgagacaaca aagaagaaag tccgaaaaat tatggatgaa
    53221 ctaggttatg ttcctaacat gactgcccgc aatctaggaa aacgcatttc cagtgccatc
    53281 ggcgtaatct taccccctct tgattcaaaa gagcggatcg gtaacccatt ttatcttgag
    53341 atcatggaag caatcaacga agaagcgcgg aattatgata tgactacagc aatcgctact
    53401 gctaaaaatt ttgaattgct gttagagaac gtccagcgta tgcatttaca aaaacaagta
    53461 gatggtttta ttctagtgta ttcagatagt caagatactg tcattgatta tttataccaa
    53521 aaccacatcc ctttcacttt gatcggacag ccttatcatc atgagaacga aatcgtttat
    53581 gtagataatg ataaccagct tctaggtaaa caagcaacag aatttttgat ccagaatgga
    53641 cataagaata tcctgtttgt gacaaacacg actcatgaaa gtttgtattt cgaacgatat
    53701 ttcggttatc aaaaagcaat gatgatggct ggtcttcctg cgttatccag tgtagtacta
    53761 gagcatccgg aggattatct gtcctttgag aaaacgttga aagaaaacga agccacagca
    53821 ttggtggtca ttgacgatat cttcgccctt cgcacgatgc agttggcgca aatgtatggc
    53881 tatgtcattc cagagacact ctccgtcatt agcttcaaca actcgatctt ctcaacatta
    53941 acacaccctt accttacaag tatcgatatc gatatctccg aattaggacg tgtcgctatg
    54001 caaaagctgc gcgaattgat ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct
    54061 catcgcttga tcaaaagaga gacagtcgtt cctttagaaa acccatacta aatagctgac
    54121 tcactaaata cgctgaccat ttatcctatg gtcagctttt tatgtaaaag aagatactac
    54181 ttctaacaca ttgtttatct gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt
    54241 ctcacgctct aaacacgtat aaacggcgga agcagaagca actccttcgg aaataagctg
    54301 aaattcacaa aaatttaaaa agcaattttc ttgagtttct tcttatttct tggagttaaa
    54361 cgcttctgtc ccagcctctt ttttccttcc ttcaattttt tacacgtcga cgattcaata
    54421 aaaaaccgcc taatattgta aagacaggtg caagaatcga gaaaaaggcg aatggcaagt
    54481 aatgcaacgc gctaactttc atcgttccca taatgaatgt gccgctgact ccccacggta
    54541 cgagagaatt gacggtcgca cctgaatccg ctagtgttcg tgtcaaatag gtttttgata
    54601 agccaacgtc atcaaaagaa cgcttgaatg tttctcctgg aagaatgatg gacagatact
    54661 gttctccaac gatcagattg attccgatac agctcaaggc agtaaacccg actaaacgag
    54721 aaggtcggtc catcttttct tttaattctt gaacgatggt atcaacgatc agatatttga
    54781 tcagtaaccc acctaatgcc aatgcaagta aaatcaaact agcagaacct aacatactca
    54841 tgatcccgcc tcgagaaagc agttcatcta gctttggatc aggtgtattt gcgacataac
    54901 cattcatcaa gatgtctgct gcttttccga ccgtcaaact tttctcatgg ataaaagaaa
    54961 gaacgagagc agctgtcgat cccgctagta atgtagggat agccggaatt tttttgattg
    55021 cccccccaaa taaaataact agtggaatca atgtccaagg agagatccaa aagctcgctt
    55081 tcatgttttg gatcatttca ttgatactag ctagatcggc tgaatgactg ttatgtccta
    55141 atatcgtata aaaaatcaac gaaaggacga atgctgggaa atccgtataa cgcataccag
    55201 cgatatgttc gaatagatta actcctccaa tacccgctgc caagttggtc gtatctgata
    55261 aaggcgagca gttattccct aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat
    55321 cattgaatcc aagtaaatga ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg
    55381 taaatgaact gccgacagtc actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa
    55441 agaaacgcac agaaacaagt ttaaatccat aaaccataat cgtagggatg gttccagata
    55501 aaatccatga agcaactaag acaccaatca aaagaaaaat caggattgga atgatccctg
    55561 gagtgattcc tttgatgatc ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc
    55621 cataaaacaa caaacacatg aaagctgcaa ggatcgggat atgtggcgag agctggaatc
    55681 cgatgatcag tactcctagg agtgccaaca acacgatcaa tacagcgata ctttctttta
    55741 aagaaatatt tttattcatc gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa
    55801 aaactattca cttggcagga cctcctacta aatcagcgac aaaacgtcgg ctgaaagact
    55861 tattttttta tttttcttct aaactttttt ctacctcaat cccccaagaa tctttcttga
    55921 agtgattgat gatcaatgga ataacgaaca tgaccactgt gatagctgct actaataatg
    55981 catcactcaa tgtcgctgta cctaatgtag caaatacgac aacgatgata gagaagatca
    56041 gcatgcagct gactacccaa gctaatccgt ttccgttttt accaattcgg taaggacgtt
    56101 cgaattctgg atgtttctta cgcattttaa gcaatgaaac agcaatcaaa atgtacacga
    56161 tacaataaat aacagttgta gcatttgtaa gtgtcaagaa tacagcactt acgtcatcca
    56221 ttacaccata aagcaaagca aataatgaaa taacgattga ttgtgttaga acgacatttc
    56281 tagatacgcc gtatttgttt tctttatgga atccgaattt tggtggaagg aatccttcac
    56341 gagctacttg gatgattgtt tttgatggtc cagtaaccca agctgataat tgtaaaagca
    56401 caccaataaa taccatgaag ctgaagatat ttccgatcac ttctggtaag cctaggattt
    56461 gacagtcaat caagataggc tgcgtgatat ttgctaattc catttttcca tctggtacga
    56521 catttgctac aagcatcgca ttgatgacat tcaataaaac taatccgatc aaagcgataa
    56581 atacgccttt tgtataattt tttgttgcgt ctttcaaacg aggcatgtaa acagaactca
    56641 tttcgatccc aacaaaaata aatgtgatac cagctaagta tttcaatgaa tcaatatgtt
    56701 ccaagtcagg gaaagctttt gaccatgaaa agtcacctag atagccgttt gagactagtc
    56761 cgactttgaa ggctgccaaa acacctaaaa cgaacatgat gactactggg atatataccc
    56821 ctagccatac accgatgttc ccgccgactt ttgccatatc gaatttcaag ttcaagatcg
    56881 tgatgatcca gtagataact aaaatacaac ctaaaacgaa ccagtgatta ttgccaagtt
    56941 ctacattacc aaatgtgttt cctaacaatg gtcctaaagt agaagcgacc attaccatac
    57001 ctgggaacat ttgcacccat aatagccatg caacgacaaa tccccatttg ctgcccagtg
    57061 ctgtttttac ccataactga ggaccgcctt cttgaggaag catcgtggaa agctcaccgg
    57121 aaaccattga tatcggaccg gcaaaaaaca aaactgcaaa aattgaataa aaaatcaatg
    57181 tccaaccgac tgcggctaac gtaggaatac tccgaaccgt tgcacacagt gccattgtca
    57241 gaccgataaa ggtcccaaga cccattttac ttacttcaga tttactcacc gttgacactc
    57301 tccttgattt tttggataaa ataacggtgt ctttgaagta gtttcagcaa taaacgaaac
    57361 aattcagcga atatttgagg ggattctttg agtttattgc ttgaagggtt tcttcaaaga
    57421 caacctcaaa acaaagggat cgaagagctt aggtctcccc ttgctcttca gatccaaaca
    57481 tgcgtcagaa acagttagta ttcaagctgt tttctaacat gattaccttg cttattttgc
    57541 ttcgcttgcc aataattggt ctgcgtagat tttttccaat ttttcttgaa gagctgcttt
    57601 gattttttca gcatattctt caaagtgttc ttctttgttc atgtatggtg tcattacgct
    57661 ggcacgcaat acagttactt ttccggcacg gttccattct tcatctgaga atcctagttg
    57721 gttcacaaat tgtaatgggc tgttgccata gtctgggata gcaaagtcag tatgtgaagt
    57781 taagaactca ttaccgtaaa tgcttccttt cacgtatgaa gaataatcat atacgtcatg
    57841 gttcaattta ttcattgcaa ctaagtcatc gttgcctttt tctttgaata catagtctac
    57901 catattgaaa tctggataag ttaatggatg aacttcgatt tctttgtctc ctactttaaa
    57961 tgacaagtca ttcaagaagt tgtagaaacg atgtgcacct tcgattgatg cacccattaa
    58021 tttaccatat cctgtgacat tcaaaggtaa gacatggtgt gctgcccaaa cgctggctgc
    58081 tgttgcccct gcttttgaac cttccaagat ataagcccca agtaatgctg ggatatctgc
    58141 gcctttttca aatacatatg tcgcaaagta agagataacg tttctcatgc ggatatcttt
    58201 gatgacgata ccaccggcag aatatggaac atagcccatt ttatgtgggt caatcgttac
    58261 agattctgct tcttcgattg ctttatatgc actgtgtact tcttctaaga tataatcttt
    58321 gttttctgta aagacattgt atttgtaatg aacatctttt aaatcttcaa atggaatgaa
    58381 gttgttgtct tcgtctaaga aaatcgcacg gccgtatccg ccataagcag catctacgtg
    58441 taagtagaag taaataccgt ctttttctaa tacgcgacgc aatgcaacga ttttatcgat
    58501 accgtcgatt gctccttctt ctgttgatcc tacaacaccg actacaccta ggattggtgt
    58561 tttttcagct gctagaccac gaacgatttt ttcaagttcg ttgatgtcca tgcgatagtt
    58621 gtggtcaact ggcactggaa tcacttggtc taaaccgata ccgatgatgt cggcagcttt
    58681 tagccatgaa tagtgtttag tttgtggaac caaccatttt cctaattttt ctaaatgttt
    58741 tccgctacgt gctgagtgag ctttgatttc gtcgattttt tctgggacgc tgtctaacag
    58801 attcatgatt tcttctgttg acaagttcat taattcccaa tcgctttttc cagcaactag
    58861 ttcaggtgtc acttctttca ttgcaagcgg caatgatttg atgttgcggg cataccaaag
    58921 tccttctaag tttgctaaag aaccatcagc aacgatgtgg ccccagccat ctttatagct
    58981 cattaatttt gcaaattcca ttcctacttc ttcttccatt tggcttgttg caggagaaga
    59041 ttcataggca acgttattac cattccatag catagcaaaa ttgtacgcta gtaaagatgg
    59101 catcaatgtt tcagagttca tatgacccca gtaacgtccg gcattatgcc aaggaacgga
    59161 atgggtacgc atccgcgctg aaatttctga tagaacatcc tttgttttat tcacagtgtg
    59221 ttcaaaacta gcactgcttt tttcttctgg cgtaataata ggcatgtctt gaggcatgta
    59281 gttttgacgc cagcctaaat gttcatccac caattcattc aacaaagctt tatagatttg
    59341 gccgttctca gctttgtccc caatgaatag ggcatttagg tttagatctt tcgacaatga
    59401 ttcactcata gtgaaaccct cctaaaaaaa tatttaaatt ttgtcgagcc ctacattaca
    59461 cctgttcttc tattctgtaa actaagtttt ggcctgattt ttaatcatat tttaatatgt
    59521 atcaattatc cgaattgttc acacttttga aataattaaa aaaaacgagg gaatttacaa
    59581 atgtaaactc cctcgcaaag attgtattaa cgacaaccag cgttctattt taggatgggt
    59641 ttcaaaattg gaatgttgta atagtatttc cctttttcaa ggtaaaaact tactaaattc
    59701 taatcaatta ctttggccaa aaagtaattt ttcttacctt ttcgaataac cacaaactta
    59761 ccatcgaatt catcggatgg atttatgaca aaatttaaat cggtaacgcg gtcaccgtta
    59821 atacttatag ctccgtttga aacatcttca cgtgcttgac gtttagatgg ttcaattttt
    59881 gtggaaacaa gcagttccac gatattttca ggtgtgcttg aaatttcaac gttcggcatt
    59941 ttaccgaatc cttgcgcgat ttcttccgca tttagttctt taatatttcc agagaacaat
    60001 gcttgcgtta ttttttgcgc ttctttcaag tcttcttcac tatgcacaaa tcttgtaact
    60061 tcttctgcca aacggcgttg tgcttcacgt ttctcaggtt ctgtctctac ttttttcgct
    60121 aagtcttcga tttcttcttg agacaagaaa gtaaagaatt tcaagtattt cactacatcg
    60181 cgatcatctt gattcaacca gaattggtag aactcaaatg gtgaagtttt cttcggatca
    60241 agccaaacgg caccgcctgc agtttttccg aatttcgttc cgtcagcctt cagcatcaat
    60301 gggatcgtta aaccgaacgc ttctgcttct ggtccttcta attttcggat taggtctaat
    60361 ccagctgtga tatttcccca ttggtctgct ccaccgattt gcaattgcac atcgtatgtt
    60421 ttatgcagat gcaaaaagtc gatagattgc aggatttggt aagtaaactc tgtaaatgag
    60481 attcctactt ccaaacggct ggcaacgata tcttttgcta acatcgtgtt gatgttgaag
    60541 tttttaccgt agtctctcaa aaattcaagt aaagagattt ttgacaacca gtcgtagttg
    60601 ttcacaaatg tgatatttgc atctttacca aacaattttc tcatttgatt ggaaagtgcg
    60661 tccacgttat gttgcacttg ttccatcgtt tgcaacacac gttctgtttt gcggccgctt
    60721 gggtcgccga tcgttcctgt tccgccgcca atcagaatat atggatgatg gcctgccaat
    60781 tggaatcttt tcatcatcat aaatggaatc aaatgtccga tatgcatcga atcaccagtt
    60841 ggatcaacgc cgcaataaag tgaaatgctc ttttcttcaa cgagctcgcg aagtctttct
    60901 tcgtttgttt gctgattaat ggcgtcgcgc caagttaatt catcaataat attcatttta
    60961 ctgacctccg tgtagttttt ttatgaaagc aggaacctgc tagcatatgt aattaattat
    61021 ttcttcagga taggatagga aataaaaaaa gtcccttggc atataagcca agggacgaaa
    61081 tctcgcggta ccacccaatt tgtatgtcta gatatccaac atacctctcg aacatttaac
    61141 gctgtcgcgt ggcaatcact gccaaagact cctgaaatgt aattcgcttt aaagcatgta
    61201 ctggtttgca gcgaccacca gctttctgaa acattggctt taacaactac ttcgtttcga
    61261 tcttcgtctt gttattatta atatgtgtct atcatagagc gcaacttttt ctttgtcaac
    61321 agaaaaagga tatttttttt aaattcctca ttttttcttt tttgaatccg ctctaattcc
    61381 gaacatttta ttttcaatta ttgttaaaag aaataacttt ttctgtattt tttctgcaat
    61441 ttatcgagaa gtcactttac tttgcctctt attctttctt cgttacaata aggaatggga
    61501 caagtaaaaa aatgaaaaag caagggacga ggtatatgga aaaaaagcaa aaaattattt
    61561 taggagccac ttctgcttta actatcgtta ttttaattct aagtttgttt tttgtcatcc
    61621 gatcatctac aaaaaaagaa ccaatcattc ctgaaaaaaa aataacagaa aaagcaaaag
    61681 aaccgacaga tgataagcaa caactagtta ctcaagcaaa aaatctggct caaagctacg
    61741 attatgacca agcgattgcg ttattggaaa aagatagtaa gaacggatct actgaaacac
    61801 aacagcttct tcaaacacta aaaaaggaga aagaacaact gatcgactgg tctgatccta
    61861 ctcagatctc tcatgtcttt ttccacagct tgatcgtgga cccgcaaaaa gccttccact
    61921 ctcaacaggc acaaggatac aaagattata tggtgactgt tgaagaattc aaccgttcga
    61981 tcgaacaact ttatcaaaat gattttgttt tagttaattt gaatgatttg atccaaaaag
    62041 acgaacaagg aaatctttcc tttaacgggc ttgcgctacc tgaaggaaaa aaaccattgg
    62101 ttctttcaca agatgatgtc agctattatg aatatatgga taattccggt tttcctagcc
    62161 gattggtcgt atcaaataac aatgaaatca aaaatgtcta taaagagaaa gataaagaag
    62221 aaatcggtga ctttgacatg gttcctctga tcgatgcctt tgtcaagaaa catcctgatt
    62281 tttcttatcg aggcgcaaaa ggtaccttgg ccctgacagg ttacaacgga gttttaggtt
    62341 atcgaacatc aaaatctcaa tatggagaca atgaaaaaac aaaaaaagag attaaagaag
    62401 ctaaaaaagt ggcggaacag ttgaagaaag acggctggag ctttgcttcc catacatggg
    62461 gtcacttgaa tatgactcaa gcttcactag aagatgtaaa aaaagacaat gaattgtggc
    62521 agcaagaagt tgcacctatc ctaggaaaaa caaatattct gatttatcca tttggtgcag
    62581 acatcagcga ctggcagcct tattcagcag aaaatcaaaa atttgcttac ttgaagagtc
    62641 aaggtttcga tattttctgt aatgtcgatg cttctacacc agcttgggga caattaggta
    62701 cggatttcta tcgaaatgct cggatcaata tcgatggcat tcgttttcaa tctgatttag
    62761 acggaaaaaa tcctattctt gatccattca tcaatgtcca tgatgtctat gacaaagaag
    62821 cacgcgaatc tgcttgattt tacgtcattt atgaaccaaa aaagccgaac tgacatttta
    62881 tcgtcagttt cggctttttt atgctttatt tcttcttcgg tgcgattccc ttgatcagga
    62941 cttctgtcca ttctgctgta tatggaagca gttttgcgta tatctccggc ttagaaattc
    63001 cttctaccat tgcttgaaaa tagaggatca agaactcatc cgaatatttc aaatccactt
    63061 ttccttcttt ccgccctcga tggaaaagat caagcatgat actgaaactt tcttgcgtat
    63121 acgcatccat cgcgtcagaa aattcttttt tttctttttt tgtatagtat gtcatcaaat
    63181 cgatataaaa ttgtttgttc actcgattga gataacggat tttgttttca ctcatggcaa
    63241 tcagtgtttc ttcaaatgga agattttccg caatcaacgc tttcgcttct tgccctaaat
    63301 cattcataaa tgcaaaaaat acttcataaa tcagcccttc cttgctgcca aaatatttaa
    63361 aaatcgtcgc tttacttacc tgagcgttct cagctacctt atccattgtc actccagcgg
    63421 ttcctatgtc tgtgttcatc aagtcaaaag ctgcttgtaa aatctctttc tttttctctt
    63481 gtgctctctt ttcaaaaccg ttcatttgta gagcctcccg taattttaaa cttttataat
    63541 atatatagtt caaatttatt ctgaggttta ttataactat ctattgagaa agtatcaagc
    63601 tagcaattcc ttgtttatga attcattgac agaaatttat aaaaagaata ttatgtacta
    63661 aaataataaa aaaagtttta taaaaaggta taaaaccttt ttattaaact atttaatctt
    63721 gtttagtata tatttttaga aaaaggagtg gggtttttat ggcagaaatc gtcaaagtca
    63781 cacatttgaa aaagagcttt ggtcgtttcg aagctttaaa agatgtctct ttcaccgtac
    63841 attcaggtga agtcgttgga tttatcggac caaatggttc tggaaaatcc acgacgattc
    63901 ggacactttt agggattata aaaaaagacg gcggaacggc aacgattttt gggcaagatg
    63961 tatgggaaga tagtttatct atccatgaac gtctttctta tgttccaggc gatatctctt
    64021 tgtggggaaa tttgactgga ggagcgatca tcgatctctt catgaagctt catggaaaag
    64081 ggagtgctca aaaaaaagat gaactgattc aacgtttctc tcttgatcct aagaaaaaag
    64141 caaaaaacta ttcaaaagga aatcgccaaa aagtgggctt gattgctgct ttggctgttg
    64201 attcggatct ctatttattt gatgagccta cttctggttt agatcctttg atggaaacga
    64261 ttttccaaga agaagttgaa aaattgaaag aacagggcaa atccatccta ctttcttctc
    64321 atattctcag tgaagtcgag cgactagcgg ataaagtcgt gatcatacgc gaaggaacac
    64381 tcgatgaaga aggaacactc gatgaattgc gtcatttgac tcgttcgacc atcactatag
    64441 aaacagaagc agagaatagt cacttagcac aattgaacgg tgtccatgac tttgtgcaaa
    64501 aaggtcacct cgctcaattt gctgctgata acgaagcttt cgatgtgatt ttagctgaag
    64561 cgtccaaatt aggtgtgaaa aaatttgaat ctattccacc gactcttgag gatttattca
    64621 tgagacatta tgaaggatga ggaggaagcg aaatgactga taaattttca cgctggtcca
    64681 cccttttttc tcaaaacctt agccgtgact ggaaaaagat cctgatatgg ctagtcggtg
    64741 tcactgcttt ttctggtggt tttgtccctg cgtttgaaga attgacttcc ggacaaggat
    64801 tagctggatt atacgaaacc ttgcaaaacc cggcaatgat tgcaatggtc ggaccaactc
    64861 cagcttcgaa tgctgctgag tatactttag gagcgatgta tgcaaatgaa atgttgcttt
    64921 tttgcagttt gtttgctgct gtgatctctg ttttacacgt ggtgagccaa acgagaaaaa
    64981 aagaagaact tggtctgatt gaacttattc gttcctttcg agtcggacgt caagcaaact
    65041 ctttagctgt catcgctgaa agtattctga tcaatttgct tttagcaata tcgatcagct
    65101 gtacgatgat ttttttccaa gcggattcga tcactgcaga aggtgcttgg ctttttgggc
    65161 tatctgtagg tagtatgggg attttgggga gtgccgttgc actgtttgct gcacaactga
    65221 tgccaagttc agccagttcg aatggtctgt ctttagcgtt tttaggtgga ctttaccttc
    65281 tacgagcaat aacagatgta gctgattcct ccctttcgat gtggaatcct ttaggctggt
    65341 cttatttgac ctatccattt acaactaaca actggattcc tttgctttat ttgtttttgt
    65401 tcagtctaat cttgataatc agttcatttg ttttagagga acatcgagat ttgaacaccg
    65461 gctatatccc cgaacgtgaa ggaaaaccgt atgctaaacg ttctctttta tccttgcgtg
    65521 gtttattatt ctatctaaat aaagggatga caatcagttg gcttatcact tttggtttga
    65581 tgggggcggc atacggctct atctacggga atatgcaaca gtttttagaa agtaacgact
    65641 taatgaaaca gatgtttatc caatctggcg tctcaattga aaaaaacttt acaagcacga
    65701 tcatggtagt cttgatttgt ctagctgcta ttttaccaat tgctagcatc aatcgtttat
    65761 ttacagaaga aacccacgga cgtatgaatc agctgttcgc aacaaaaatt tctaggaacc
    65821 aactttattg gaccaatatg ttactagcta ttctatttgc tatattggca atcctgatta
    65881 gttctggtgc actagggctt tctgctttgg cttcgatgaa ggatgcgaaa gacatggatc
    65941 tattgatgtt tcttaaagcc gggttcaatt tcctgcctgc cgttttagtg tttatcggaa
    66001 ttagtggctt tctattcgga tggcttcctc gaatcggtaa acttgcttat atctacttag
    66061 cttactcctt tgcactcaat tactttggcg gaattttgga tttaccagaa tggttttcca
    66121 aaacagctgt ccctagctgg gttccacgga tgccaaacga ttcttttgat ctttccgtgt
    66181 tccttacgtt atcatgtatc agccttgcac tgttcgtcct tggttctatt ggttataggc
    66241 gaagagactt actagaagga taataaaaaa gcatgtacaa tttcatgcta gcctccaaat
    66301 ctgggtcaaa aatccaacat ttggaggtca tttttttatt tacaagtctt ctaactgctc
    66361 tttatcaatg atccagactt ctcgattctt tctttttaca taaccattct cctctaataa
    66421 tttaaatttt ctagataaag tttcgggggt cgtaccgata aatgcagcaa gttctttcat
    66481 tttcattggc aggaaaaagt gatccttccc tgtcgcttta gctaagtcaa gcagatagac
    66541 agcgactcgt tcttctactt tttccatcat caaaaaagtt gtctgctgct gtacttttgt
    66601 taatttctca acgttgattg ctaaaagttt cagactaagg gcgggataat gcagtaagag
    66661 ttccgagaaa tctttttgtc tcaatatgca aatttccgtt ttttctagtg cttcgccaaa
    66721 taaagactca tttggaacgc ctagcaactg gctctctccc tcatatccgc ccggctctat
    66781 tactcgtaaa agctgttcct ttccgctagc agataattga tacactttca tactgccgga
    66841 ggccacgatc actaattgag gttctccatt cggcgcaaga atctgttctc ctttttccac
    66901 atagtgatgt ttgacaagtg cgtgtacttt ttcttgatct tgctgtacta agtgattgaa
    66961 caaaggaaca aatgaaacac ataaatgatc atgactcata atctctcctc cttctaaaca
    67021 gctatttcta ataaagaaac ataacttccc ttattccaac tgataatttt cttttatcat
    67081 agcataaacg tcaagcgaac cagataccag caaatagagt ggatttcttt ttgtcccttg
    67141 ctttattttc cttcttttga gaccttaagt aattgattcc tatcaatttt ttaaatattt
    67201 actttttgaa taatcaaaaa caaaccttca ctcgcaagcc gagagtaagg tttgtttttc
    67261 gcattgctta tttcgtgttg tctattgaaa tttttccgca cctagctttt gatatagtgg
    67321 tgctagttct tgtaaggttt cagcggttat ttgctcaacg tgttcgattt cttgcggata
    67381 gaagaatagt ttacccattt gaaaggttcg gtctctcatc gcttttttca actcttcttg
    67441 tttctttgta tcggacaaat agtcagacaa agctaagtgg tcgtcaagtt catgttctgg
    67501 ttgcggccat atgtaataat ccttgatatc gactgttttg ctccactctt tcagtatcgc
    67561 aagcaactgg ttatacgctg caatgctacc atatcgttct tcgcttttat aatgttggta
    67621 catcaaatag atttggtatt gtttttgatt caacaaaact gctagacagg cattctcgtc
    67681 ttgacgagat tcgcttcggt acgcgctcca aaaatgactg cgtaaattcc agccattcgt
    67741 ccagctttcg attttaggtt tgctcatgcc gtacatatca ggcaaaagtg cagctgtctg
    67801 gagctgaatc tctttccatt tttgccagtg ttctttatat tctttcttga tttgttcaat
    67861 cttatctgtt ggatggctct ctttgagctg tttgaatgcg tagattggtc gatcaaacaa
    67921 ctgataatct gattttccaa gttccattgt ttcgcctctt tccgttatct tttgtattct
    67981 acctgaattt tgcgatatgg acaaatagag gttgtgaaaa agctgttctg catcaagtca
    68041 taagaacagc caaacaaaaa actgcgatga gcatcaccgc agttttattg aacttaaggt
    68101 tgattgatca catctaaaag cgttgaaaat aaaatggcac tagaagcagc tcctgggtcg
    68161 atatgtccga ttgcacgctc tcctagatag gaagctcgtc cttttttagc taccaagact
    68221 ttggtgtgtt cttttgcctg gtcaatgatt tcttttgtta aattccctgc cttcaatgcc
    68281 tctgtgactg gcagccaaac atctaacatc gttttatcgt cagcttctgc ctttcctcgg
    68341 ctttgaatac ccgctgttcc ctgctcgatc acttcgccta atttttcttg agaatcaata
    68401 gaatcgacct cttttgtcgc tttcatcatg ttcatgaatg cagatccgta taacggacca
    68461 gaagccccac cgactttcga tatcatcgcc atagagaata ctttgaatgt atcgctgatt
    68521 gtttcaggat gatcggcttg aaaagcttct tcataagctg aaacccctct tcccatgtta
    68581 tttccatggt ctccatcgcc aatcggggta tctagatcac tgagatattg tttgttctga
    68641 ttgattgcct ctgcaaattg cgtgagccat tcttggattt cttgaacggt tagtttcata
    68701 ttatcaccct tttatcttga gattttttac catgaaatcg tctgtacatt gctttctaaa
    68761 gaatctttcc attgtgtctc cgtcaagtcg atcagtgtta gagaaagacc ttgcatatct
    68821 agtgaggtca taaaattacc gacttttcgg aaaactacat tgacgcctct ttcttccaac
    68881 agagctaaaa catcattcat gaacacgaac tgttccatca aaggcgtccc gcccattccg
    68941 ttgactagta cacctgcttc ttttggtaaa ttcgagtaat catctaaaat cttagtaaca
    69001 agttcctttg ccagtacttt agaaggctgc atcttttctc gacgatagcc tggctcaccg
    69061 tggataccaa caccatactc gatctcatct tctcctagtt cgaatcctgg tttccctact
    69121 tctggtacag ttgctgctcg aagcgctaga ccgatcgtct tagttgcctg tacgaccttt
    69181 tctcccagtt ctttcaactc agctagggaa gcaccgtttc ttgctgcatc gcccacgatt
    69241 ttgtgtacaa ggacagtacc tgcaactccg cgttttccag cagtatacgt actattttca
    69301 acggcgatat catcatctac gatcacactt tctacttcaa tatcgtccat agcagctaat
    69361 tcctttgcca tatcgaagtt caatgcatcc cctgtataat tcttaacgat cagcaaaact
    69421 ccttttccac tatcggcttc cttaatagcc gtctgaatct gatctggagt tggagaagta
    69481 aacacatcac caagcaccgc tgcacttaac atcccttccc ctacaaaccc ggcatgggat
    69541 ggttcatgtc cgcttcctcc accagaaacc aaaccaactt gttccgcttt ctgattcttg
    69601 gctacaacac gggaagattc tacacgatga acaagttctg gatagctttt tactaatcct
    69661 tccagcattt cttcgacaat attgcttgga tcattcatga tttttttcaa tttattccca
    69721 tcctttcttt ccggcatttt gtgcttctgt taagatttgc tgagtatcat ctgttatacc
    69781 ggcagtgatg gctgctgcaa aagctccttc cactaaaggc gcatccacca gatgataacg
    69841 ttgctgctgc tcttcttcca acatgtcttt tgccagttcc gagttcaata ccgcgctacc
    69901 gatatccgca aaaacaaaat aactagctgc atcgcttgat tcattgactg cttctacgat
    69961 tttcatcggg tctgatccga tctcgccatt tattccgccg agagaaaaaa tttgcacctc
    70021 ttcggatttt tgcatctgct caatcatttc tttaatacca tctgttactt tttgactatg
    70081 agaaataagc aaaatacttt ttttcacaag aatcttcctt tcttttacat agttgctaac
    70141 gattggtttc gctttcatta atgaacaatt caagtatata ataaatttct aattttggga
    70201 aaatatgtgt agtgcttctt gaagtttgtt atactttaat taaaagagag gagtgatcga
    70261 atgattacaa caacaacgaa cagtgtagag ggaaaagccg tagtagctta taaagggatc
    70321 gtttttggtg aggtcattac tgggatccat gcatttaaag acttggaggc ggggttgcga
    70381 aatatctttg gcggccgctc aaaaagctat gaaaacgaac taatgggtgc aagagaagaa
    70441 gcacttgccg aaatggccga acgtgcaaaa gcattaggag ctgatgcgat cattggcgta
    70501 aaaatggact atgaagtatt aggcagcgac aatggcatgc tgatggtcac atgtagtgga
    70561 acagctgtct ccacgagata agtaggggac atctgtttta ataactaaag agagatggga
    70621 catgtagtgg cactcaagaa ttagattttt cagtctaact tcgaggtact ctaccttccc
    70681 atcctctctt ttttatttct ttttgcggat gattccgtaa gctgctgcga ctaacacacc
    70741 cgcccctgct aaggaataac tgacagaagc tgttttttca tttgtttttg gcaaagtctg
    70801 acttgttgat ttggatggct ctgtcgtttg ctgaactgtt tctgtcgtgc ttccagaggt
    70861 cactgatttt tctcctgccg aatgcaaaat atcttttatt cgttggaaac tagctgctaa
    70921 gtcagctttt tttgcttcaa aatcattagc aactgtttgt gcttcgctga tctttccgtg
    70981 gattgcttga ctggccgctg tgatatccgc ttgagtttga cttagctttc cagaagctgt
    71041 ttgagcaacc tgatttaatt gctgattgaa ttcttttgct tgttggatct tcccgtcaag
    71101 atctgtttgg acagcttccg cttttgcttt cgcttcggca agctttttct caagttgtgc
    71161 acggtcattt cccactttat ttttaagatc agctaaatct tgatcgattg ctgcttggtc
    71221 ttctttcaat ttgttttttg cagcttctaa tgcttcttcg agttcttttc ttttattttc
    71281 caagtcttgc aaatgatcga tttggtcaga agaggtagaa ctatttattg atgtatcttc
    71341 tgaagatgaa atcgtttctc ctgaattatc tgtcggtaca ttggaagtca tttctgtttc
    71401 cgaggcaccg ttttcagcac cagaagagct tatggttgtt gaaggttctg tttgagcagg
    71461 ttcagaagtt tccgcaaaag ctgtctgtcc caataagaaa atactggaaa cagcaattgg
    71521 ccagattaac ggcttcattc tttttttcat cacttttccc atccttacta ctttttaact
    71581 aatttcttat tataagcaaa aatgtttgga agaaccataa acacaacagg ctgtttgtct
    71641 aaaaaacggt gcatctttta tttttttgta cgttccgggc ttttccatgt cccgcttcca
    71701 taacctaaaa tcgttttgac tgctggaatg aaagtcgtca cgatcgttat cgcgttcgct
    71761 atccagaaca aaagcatata tagtggtgcg aaaagaaggt atttcaattt tcttcgtcga
    71821 tcatctacaa tcagcgaagc tagcaactgg aaaaatcctg cgaccatttc aaagcagatg
    71881 aacacaaaac tgaatgtaaa agtaatataa atctgttcgt agtttccttg atatacataa
    71941 tgcccgatta atccagcaaa aagaactacg gaaatacaaa agaatattga ccagacaata
    72001 ctcaatgttt gatcaatgaa gatgatcgtt cttccaatat gtttaaaagg atggagcatc
    72061 acttttttga aattcgtcag ccagacttct gttccacctt ttgcccaacg ttttctgtgt
    72121 cgatatagca tcttcaacgt cacaggtact tccatgaaaa agataatctc aggagcaaaa
    72181 acagataacc agtcatttaa ctgatgatcc caagcaatac tgatgtcttc tgtcgctcga
    72241 tcttgtctga atccgcccac atcgatcaag gcttcttttc gatacatcgt atttgctcca
    72301 ctgtacgcat aaagaccacc gaaaacagct gactgtgttc gtttaatgat gcccacgata
    72361 cttgagaatt caaccgtttg agattttgca atcaatttgg tacgattttg gacatccata
    72421 tttgcagtga ccgcagcaat atgtcttgca ccagggcgaa tgaaataatt cacataccta
    72481 atcagtgcat ctggctccgg taccgtgtct gcatcattgc ttaaaatcaa tttccctttt
    72541 gcaaaggcaa gtccgatatt aaacgcgtgg gcttttcctt tatttttttc gatccgaacc
    72601 acacgaagat tagcgtattt tttcatcagt ctagctaata tttctggtgt ttggtccgtt
    72661 gaaccatcgt ctgtcactaa tacctcatag ttgtgataat tcaatttcgt catcaaatac
    72721 tcgattgtat attcgatcac aatttcttca ttatgggctg gcaccatgat cgtgatgaac
    72781 ggttcgacac tcaagggaac atctacccat tctttttgct tatgtttgta taaaaacaca
    72841 taacaccaga caccaataaa ccatacaaat ccgcctaaaa tcggataaga aacgagaaaa
    72901 aaatacataa tagacaaaca agtatctaag aataaattac ctgtatgggt gattgagatc
    72961 atttcagttc caccccattt tctttataca aatctgcaat gaatgtcgta tctagatttt
    73021 tctcttcgga gacagaataa aaacggaccg tttctctctc ttcttttgtt ccaaaacgtt
    73081 ccgtatacac ttcttcaagt aattcttgtc tttttttcaa tttttcatct tcatattcaa
    73141 aagatttatt tacttttttg tatagatttc gattgttcca cctagtcaat acaaatgagg
    73201 taatcaggaa actgaaaaaa atcaatacaa aaaagccgat taagaaatac agcattttct
    73261 tttctgataa aaaagcgtac aaggaaaaat ccttggctct ttctggtctc gttaatggga
    73321 aaaagaccca tataaaagga agaacaacga tcatccatcc taaaagagcc actaacgttt
    73381 gccaaatctt taaccagtaa ttaccttttg aaaaatactt atctttcacc aaaattgatg
    73441 caacttcttc tcttgttact tttttcatat cctcacctca cgtttttttg ttacgacgta
    73501 ggagtttacc atacatttca tttcttttca cataaagtgt tcctaaattt ttcctaagaa
    73561 aaagaacaaa acatttctca tcagcaaatg aaaaatattt tgttcttttt atcagttgtt
    73621 ctttttattt tttatctctt aattatgttg ctctttccaa tacgacaatg cttttcattc
    73681 ctctagatat cgccgtgtac atttgtctgc gtcttctttg ctcatctcca tcgttttcaa
    73741 atggatataa gattacatga tcaaattcca gtccttttgc tagactcaaa ggaatgattt
    73801 ggcagttttc taatccatat gaattttttt taatcgattc ttgtagcgtt tgggtttcct
    73861 cttctgtttg ggtgataact gccgcttttt ctccttttag agaaaccaaa atctcagcta
    73921 gtattttcaa caattcttca gaggatcgaa aagagcggta ttcgggttct tttccttgcg
    73981 gtcggatcga tacgatgtcg atcttctctt gatccacagc ataggttttg aaaagctctg
    74041 tgattgctcc gctagaacgg taactatttc taagatcata ccgatggatc ggcagatggt
    74101 agtcgtcgaa acaacgcata atatcagcaa aagtagtgga agaattaaag attgcctgat
    74161 tctcgtcacc tactaaagta aagtgagttt ttggaaataa ttcgatcaat agacctaatt
    74221 gtgccagggt ataatcttgt acttcatcaa tcaatagata gcgaaatgct ggtacattta
    74281 ccttttcaac aagtaaatga cgaatagcta atataatgac tgcttcatca acagtacgtg
    74341 tgggttgcca agcataagct gagccatatc gtttttcata aattctttca aacaactgat
    74401 gctcatccac ccatgccatt tcttcgattt gtctggttat ttttcgatat tttttttgta
    74461 aaagtttcct tgcataagct acaatacttt gttccgaatc atcagaaatc aatttaccga
    74521 ataattccaa ctgcctatct tctgtcaacg agagtacctg attctgcaca gcaggacttt
    74581 ttgcttgttt caaaagatga ttcttccatt cgctcatcag tgcctttttc gttgcttgaa
    74641 gcctatcgat caatttagga tgagcaggaa ctttctcata tattttttcg atcgtttttt
    74701 tagaaaggac tgtttttcca tttttagtaa gtccacggat aaaattcgga tgatctaaaa
    74761 acaaaggatc tgattgtttt agattatcga taaacttttt gctacgcaaa cgttctgtct
    74821 gctccgatac attttctccc gtgatacgtt tgaaataagc ctcttcacct tctatttcct
    74881 ctgctgaaag ctgtgaaacc agctgtatga tggttagatt cagtggattt ctttcaccta
    74941 atgatggtag gacattcgaa atatagtcga taaaccgatg attaggtgat agaatcagca
    75001 agtcatcgga tgtcatcgta tctcgatata aatagagcag atacgcaata cgctgcatga
    75061 tagcagaggt tttaccactt cctgctacgc catttaccaa gatattttga cttttcacat
    75121 cccgaatgat tgtgttttgt tccgattgga tcgtagcagt gatagccttc atttcattgg
    75181 tctcatcttg gcctaagact tctaataata cgtcatcttg aatagctaca gacgtatcaa
    75241 aaaacttgac caaacgatct ctttccaaaa gaaattgtcg tctttcttta ataaccgtat
    75301 cgattttatt ttgacgaacc gtataagaag atggaccaat catgttattg taaaaaagtt
    75361 cggcaattgg tgaacgccaa tcataaacaa gattttcatc tgcatcatct gtgaaatttg
    75421 ctgtaccgat ataaaaggat tcttttggct cattatccag aaagtctaca acgacttttc
    75481 caaagtaggg acttttcaat aaacgttcga tctttgctag ctgattttct gcgtttttct
    75541 gtttgatatt catctgatcg atttctctat ttttcatttc aatcatcgaa aaagtatcca
    75601 ggttatctaa gtaactgtca aaattcaatc gaagctcgcc agccatttct ttcaacgact
    75661 ggtttccttc tatttttgcc gtatccatcg attcccgcag ttcgtttttt ttatcaatta
    75721 gcttttgata cacgttcttc agatatgctt cttctaactg tttttgtgtg ttgaccatac
    75781 acattctcct ctttttttaa agtggtggaa ccgattagtt tacctctttt aagagtcgaa
    75841 tgcaagcttt ttaacgagta tcatttgtta ggatatatca gttcttcagg tcggacagtt
    75901 tgatataaag aaaatgcacc atcaaggttt tttacgtggt agccagactg cttcaagatt
    75961 ctttccgcta gatagctacg taatccgcta tgacagctga cgatatattc ttgttggctg
    76021 tctaattcat ttaatcggtc ccgaagttca ttcaaaggga tatgtttggc ttccttgaat
    76081 ttgccctttt ccaattcttc tgctgttcgc acatctaata aaatttttcc ttttgctagt
    76141 tctgtcggca attcatgcca ttgtacggtt tcactaagcc cttctgcaat gttcattgct
    76201 gcatatccta gcatgtttac tggatctttg gctgagccaa atggcggtgc atacgtcaat
    76261 tctaattccg gtaaatcgaa gatcgtcaaa tgccctttga ttgctgttgc taaaatatcg
    76321 atccgtttat ccacaccttt tgccccaaca ccttgtgcac cataaatctc gccagtctct
    76381 ggatggaaga tcaacttcag taaaatatct gttgcttctg gataatagct tgcatgatct
    76441 tttcctgata catgaatgac ttcaaaagac agttcgtttt ctttggctgt tctttcactt
    76501 aaaccggtgg atgcggcagt cattccaaac gttcgtataa ttgctgttcc aataccgcct
    76561 tgatttcttc gagaaattcc ggaaatcgtg tccgcaactt gtctaccttg acgattggct
    76621 ggtgaagcaa gagaaataag tgcatctttt ccagtgattt gctgttttac aacgatagca
    76681 tcccccaccg cataaatatc aggaatattc gtttgataac gttcgtctac taggataccc
    76741 ccgcgtatgc ctaattcgat tccagcggtc actgctagct ggttatcagg acgaacacca
    76801 atggataaaa taactaaatc agcagggatg gtacgcccat catccagtcg caatcgtttc
    76861 ccgtggtctt caaatccagc gacagcatga gaagtaatca cttctactcc ttgatgagac
    76921 aattcttttt ctataaaagc tgccatttct tcatccagtg ttggaagaat atgtggtgct
    76981 tgttcgatca ccatgacttc taaacctctt ctttttaggt tttctgccat ttccaatccg
    77041 atgaatcctg caccaacgat cacggcacgc tttggctgtt tttctaatgc atgcatcact
    77101 ttatcgatat ctggtacatt tctaatagaa aaaacattgt cggcttctgt tatccctgga
    77161 atcgatggaa caactggttt tgcaccagga gataaaacca atgcatcgta ctgttcaatg
    77221 tgtttttgac cctcatgtac gacttctacc gttttgtttt cgggaaagat ggctgtcact
    77281 tcatgatttg gacgcacatc taaacaaaaa cgagcggata atgattctgg cgtttggaca
    77341 agaagatttt cccgttcact gatttctccc gaaagataat aaggcaagcc gcaatttgca
    77401 aaagaaacat acggtccttt ttcaaaaaca acgatttctg catcttccat taatcggcga
    77461 agccgtgtag cagcagacat tccacctgct acacctccga caataacgat tttcatctta
    77521 tttccctcct actgttcgat caaaccattg attcatgcca ccacgtacat tcactacatc
    77581 ataaccagat ttctttaatt ctttagctgc ttgtttactc ctcatccctg attgacaaat
    77641 cacatagacg gtcttttctt tgtctccatc atatctgttt atactttgta atggaacatt
    77701 tttagctcct tttatatgcc cgccccggta ttctgcaggt gttctaacat caagtaacgc
    77761 tggattttcc aaaagctttt cctttaattc tgggactgtt atcgatttta ttgattgaaa
    77821 tagcaaattc attttctgca cctcatttac atttttataa atacctcatg gggtatatta
    77881 aacaggagct ttattaatat gtcaaccaga atgactcttc atttttttca ctacttttta
    77941 tatttgcata aaataccgat aaggggtatt atctaattat aaaaatagag gagaatgtgt
    78001 tatggagatt gataataaaa atatcctgaa ccgtttgaaa cgtacagagg gacaaattcg
    78061 tggtatccaa aaaatgatcg aagacgaaaa agaatgtatg gatgtcatca ctcaattaag
    78121 cgctgttcgt tctagtattg atcgcgtaat gggcatgatc gttgcagata atctgaaaaa
    78181 ttgctttgag aaacctgata gtaatccaga agaacaagcg gctaaactag aacaagcgat
    78241 caagatgatt attaaaaagt aaaggaatga tttcaatgaa agtcgttttc catattgatg
    78301 aattagaaaa atggtcagaa acagggaaaa acgtaaaaaa cctgataaaa gcttctccag
    78361 aaacgacgat tgtcgtatct gtaaatggca tcgcgattac cggttatcta gactctgcca
    78421 atgcagaatt cttagatttg caaggagtcg tttttcacgc ttgcgccaat gctatgcgtg
    78481 ctaatcatat ttcagaaagt tctttacctg aacaagtaat cgttgtacca gcaggtgtcc
    78541 ttgatttagt tgaactgcaa tcccaaggtt atgcttatat caagccttag tcagtagagg
    78601 ttgcgacaga agtgatcagc ttcaagaaat ttcggcttat ttttcgaaga agctacttct
    78661 ggaaaaccgt gaatcaaagg ttgtgaaaac gctgtcctgc atcaagtaat aagaacagcc
    78721 aaacaaaaat agcttctcat atttttcgtt tggttgggct tattaccgca gatgcaagct
    78781 tttgaacacc gtttaaggaa gaggttgtga aatcagcggt ttgacctaag tattagagaa
    78841 aaaaacggga aaatagctcc tcatattttg ccgttttttg agctaatacc gaaggtcagc
    78901 tgattgaaca ccgtttaagg aagagattgt gaaaaagtcg cttagtttcg agtattaaga
    78961 aaaattttgt gaaaatggat ttctacattt ttgcataaat ttggcttaat accgagaaac
    79021 tggcttttga ataccgttta aggaagaggt tgtaaaatca gcggtttgac ctgagtatta
    79081 gagaaacaaa cggaaaaata gctgctcata ttttgccgtt ttttgagcta ataccgaagg
    79141 tcagctgatt gaacaccgtt taccaagagg ttgtgagcct accatttaac tccgaatcac
    79201 aagaaataat ttcaaaaaca acttctcctg tttttggttc ggttggctta ttatcgagaa
    79261 gtagctactg ggagtctatt tatcaaaaaa aggaatgcga cataggtcac accccttttt
    79321 tattttcttt gaaacatctt tcggtactcg acagcataca agacaaaaga ttgtaaaaga
    79381 ccgtaagtga tgacggcaaa aatgactctc acccacagcg ttggtaattc tgggaaaagc
    79441 agcaaaagga tcatgccaac atagatgaca gcagtcgcta ctttgccata ccacttcgct
    79501 ccacttaatt tgatattttt agatagcccg attaatccag ctacaaacat aaagccttct
    79561 tttatcaaga atagaccaaa caacagccaa agccacggtc gataccatgc catagacaaa
    79621 atcagaaaaa gttgtgtcaa tttatctgca aacggatcaa gcaccttccc taagtctgtt
    79681 acctgattat atctccgagc aatgaccccg tctagataat cagacaaacc tgagacaaca
    79741 atgaccgctc ctgctatata aaatgactgt ctcagcacaa aataaagata aatcggcagc
    79801 aaaaaaatac ggaatatcga taaaagattc ggaatcgttt gtatttcttt acgccagtct
    79861 gtgcttaatt tcattttatc gccctacctc ctcagcactc ttttcttttt attttagctt
    79921 acacggggaa taaaaccaat gatttcactg tttttatttt tctttacaga cactgatatt
    79981 atgagctaat ttgagataat cacttgctgt tttcatcgtt tactcttctt tcttcctgtc
    80041 gtttgcgcat ttcttttact agtactttat gagctttttt tgatcctcgc tctaggtcac
    80101 gctgccgttt acgtgtttta taatcgataa acagactgtc taaatcgtaa cctgtcgggt
    80161 acagatcttc tgctgaacgc accagacgaa cacgctgacg aggcacgtgg attatttttt
    80221 tgtctaaata tacatcaatc gtatcttcac ctatatcttc ataaactaac gcttcttttt
    80281 ggtattcatt gacaaatatc cgatcacctt ttgcaaagag ctgacgttct tttttattct
    80341 ccttcctatt tgtaactgct gtcttaggaa gaaattcttt tttctgtgtc cgataggctt
    80401 tttccgccaa gtaacgctct gcttgatgga tcaatttcat cgacatatcc attttatgag
    80461 caatccacaa agcttggctt tctcctgttt cttccaaccc gcaactgata tttaggacga
    80521 agtgcttccc gatcgaaagc catagcagcc ggcacaaaat cttcatgatc tccggcgaat
    80581 ttcttgattt ctccataatg ggtcgtggca atgatcaatg ccccttgctc atacatagat
    80641 tccatgattg cgatcgctag tgcagcccct tcatttggct ctgttccact gccaatctca
    80701 tctaaaagta ccaacgtatt cctttttatc tttttcaaaa tagcagcgat gttttgcata
    80761 tgaccagaaa atgtactcaa cgcattttct aaattttggt ggtcaccgac atcgacgaaa
    80821 atctcatcaa agacagcaag ctctgtccct tcttttgccg gaacttgcat accgaacatc
    80881 gccatcaaag tcaataaacc gactgtcttc aacacgactg ttttgcctcc ggcatttgct
    80941 ccggtgataa tcaagcctcg atagtcttta ccgatttcaa catccaatgg taccgcatgc
    81001 tcaggtaaaa atggatgtct gccttgcttg ataatgatat gttccgattt attgattcta
    81061 ggtgtgcttc cattgatttc tcggctaaat tttgcccgag caaagatgat atctaacacc
    81121 gtgatcgttt cgatcaataa atcgatcact tcttcgtttt ctgcgatagc accagtcaat
    81181 gccgccagga cttgatattc ttcactgatt tcttcagctt ttaacaactg atagtgttca
    81241 ttcaatttag aaatcacagt tggttcaata aaaaccgtcg ttcttttatt tgattcatcg
    81301 ataatcgttc catctatttt atttttatag ctggctttga taggaatcgt atagtattcg
    81361 cctttttgaa caatcattgc ttcttggatc atttctttgt ttttcggatg acgcaagaat
    81421 ttcagtaatt ttgactggat ttctttttcg attagttgta attgtttgcg tactttacgc
    81481 aaattacgtg acgcgtcgtc actgacttta ttatttttta ttttctggtc aatcaactct
    81541 tctacattta tcaaatcagg aagatgtttg ctataagcaa acaacaaggg tgtctggtat
    81601 tgattcttat caaaaaactt tgtgatcatc cgactgcttc taagaaaatc cgcatattcg
    81661 atcaaatcgg ttggctgcag aatcaaccct tttttgactt gttctgtcag tgcatcgatc
    81721 ctcggcaacc ccataaatgg aacatgctga ctgctttcta agatcaagcg tgcttctctt
    81781 gtttcttctt gacgatccaa gacagtttgt aggtttgtcg agaccgtcat atcgctgatt
    81841 cgttttttgc tatagtttcc aattgctctt gcttggattt cctttttgat ccgatcaaat
    81901 tgtaattgac taatcatttc gttgtccata ttctctcctt ttcttgaaga gatacctgtc
    81961 ccaaaataaa taagggccta gagataaata aaaggataga acacagctta aaaaagtcat
    82021 gtcctatcct tattagaata caaagatatt gttttcctga caggtaccgt ttcataagca
    82081 caccaaataa actttgttta ttggctattc gacttactga ttagatacca gctacactca
    82141 caaaacatac cccattcttc taggtcttat ttattttcca cttaactata gcaagacaca
    82201 ttttggttgt caaactttag tatgttttat agatttacac cattaatgaa tagacaccta
    82261 ccgcgataat tgctccgatc attggacctg cgattggtac ccatgaatat gcccaatctg
    82321 aagttccttt attagctatt ggcaataccg catgtgctaa tcgaggacca aagtcccgag
    82381 cagggttgat tgcatatcct gttggccctc ctaatgaaag accgatggct aagatcaatc
    82441 cacctacagc gaatacgttc gttccaggtg caaattcgtt ttgtccaaaa gctagcaacc
    82501 caagaactaa tacaaatgta ccaattaatt ctgtgatcac atttgctgga taattccgta
    82561 tagcaggacc tgttgcaaac gttcctaaaa tcgcgctttc atctttcgtt gcattccaat
    82621 gtggcaaata agaaagccaa acaagaatgg cccctgcaaa agctcctaaa acttgcgcaa
    82681 tgatgaaagg aacgacaaga ttccaactaa ttgcccctgt catcgccata gctagagaga
    82741 ctgcaggatt caaatgagct gggcccatga atccagaaat atagacagcc attgttacag
    82801 cgagtcccca accaaatgcg atgacaatcc agccagaagc ctgtgcttta cttttgttta
    82861 aattcacagc cgcacacaca ccatctccaa gcaaaactag gatcagtgtc ccaataaact
    82921 cccccatgat ttgagtcata tcactgttca tttatttctc cccctttagt tcccgcagat
    82981 ctgattctgc aatcaatgct tctaaacgtt tagtctgttg cgccttctcc tcttctgtcc
    83041 attcaaaata tccagcaatt gcatcgatca caggttgctt gatctcatca agctgatctc
    83101 gttcaaacaa cagatgattt gtacgacgaa tgagataatc gcctggggcc aaaaccattt
    83161 cttcttctaa accataacgt aaccgagctg actcagcgag actcaaacca ggataaggtg
    83221 ccatttcttt tgctaattca aagatacgtc gggcattagt cccgtaaaaa tcagcgatat
    83281 aagtagcatc ttcttcctct aaaccggctg ctactccttc ttttgctaat tctgtcactg
    83341 tttcttctaa tttagttgga tcgaagtttc cacctgaaat ctgatatttt ttagagtcga
    83401 tttctttcgt ctctattccg tattcttctt ttaataattg gcgtatcaat cgcaaagctc
    83461 cttctgccat cttacggtaa tcggtgattt tcccaccaga taaagtcagc aaaccatctg
    83521 gttctctttc taaagaacta cctctggaaa tcgtagaagg tgctttttca tcacggctgt
    83581 tttccaaatg gttcaacacg tcttctactt cagcacgaga aactttgttt tccttatatt
    83641 cacttactgt atcgacaact ttattgaagc tcttgtctga aatcgatcca ttatctccac
    83701 cattgtaatc agaaccagaa ttaccaatca acagtggacg aagtcctgcc cagcttgctt
    83761 cgatatcatt caatgtgata tttgcttctg gatagcgatg attgatcaca tccaatagat
    83821 aatctacatc ttcttgtgtg actttcggat ccgtaaaatc tccttggtaa tccgtatctg
    83881 tcgtaccaaa gtaagtcttg ttttctcttg gaatagcaaa aaccatccgc ccatcttgct
    83941 ttcctgtatc gaagtatgtg ggttgcggta caggcagttt tttcgcatct acaactaaat
    84001 ggatcccttt agttggacgc attttaggag agactggacg cgtaaaattc aagtttcgta
    84061 ctttatctac ccaaggacca ctcgtattga tcactaattt tgctttgatt tcgatcactt
    84121 catctgtcag caggtcacga gctttgacgc caacgatttg atcgccatca tataaaaagc
    84181 caaccgcttt cattttactt actagataag ccccatcttc ggcagccttt ttgatattat
    84241 caatcactaa acgggcatca ttgttgcgga aatctagata gacacccgcg ccttttagcc
    84301 cttctttttt caaaaacggt tctctttcca atacttcttc tggtgtcagc gtataattct
    84361 catatttggt tcctgtcaca tttgccagtt tgtcataaag gtccattgct actttgaccg
    84421 agaacatatt gaaggttgtt gctccttcgc cctcatagat tggcaaaagc attggatctg
    84481 gttttgggat gtgtggggca atactttgta cgactgcacg ttcaccaact gtgtcagcta
    84541 ctacttccac atcaaatgtc ttcagataac gaataccgcc atgaacaagt ttggtcgagc
    84601 gagaggaagt cccttctgca aaatcctgca tttcgatcaa tcctgttttg attcctgatg
    84661 ctgctgactg gactgccaca ccggcaccag tgattccgcc accgatgatc aacagatcca
    84721 attcttcttg ctgcattttt tgaatgctat cttgtcttgt cttgtttgaa aacatatgga
    84781 tttcctcctt attcaaatgc ttgggtagct tttactgctt ttttccaacc gttgtaaagt
    84841 ttttctcggc gctcttcctc catttgaact tcaaatagct tgccagcttc ataaaattcg
    84901 cggatctcgt ctgtatcttt ccaataaccg acagctaatc ctgctaaaaa tgctgcccct
    84961 agcgccgttg tttccaagtt ttctgctcgt tggatcggta cattcaagat atctgtttgg
    85021 aattgcatca gatattcatt gtttgctgca ccgccatcta ctttcaatac aggaatcttg
    85081 attccagtgt catcttgcat cgtatcgatg atatctctca cttgataagc aatcgactgt
    85141 aaagtagctt taatgatatc ttcttttgtc gtaccacgtg tcaagccaaa catagcccct
    85201 cgtgctgcct gatcccaata aggagcacct agcccaacga atgcaggaac aacataaacc
    85261 tcatcttcgc ttgtagagcg tttcgctgcg tcttctgaat cgctagcttt ttgcaacatt
    85321 tgaagtccat ctctcagcca ttgaatcgaa gaaccagcca caaaaatact tccttctaaa
    85381 gcatagtaaa cctttccatt tatgccgtaa ccaattgtcg tcaataaatt atttttggat
    85441 aattgcggtt tttcaccggt attcatgaca ataaatgaac ctgtaccata agtattcttg
    85501 accatgcctg gttcgaatgc catttgtccg aataatgcgg cttgctggtc tcctgccatc
    85561 cctgaaattg gtgcttcgct tccataaaaa tgataacctt gcgtataacc ataaacttct
    85621 gagttagaag tgacttttgg taacatcact cgaggaatat ttagtaaatc taaaatctcc
    85681 tgatcccaat ctagctgatg gatattgaac aacatcgtcc gactggcatt cgagtaatca
    85741 gtaacatgtg cttgtccatc ggtcagtttc catactaacc aagaatcgat cgttccaaac
    85801 atcaattcgc ctttttcagc tcgttcttgc gcgccttcta catgatcaag aatccagcgt
    85861 atcttcgttg ctgagaaata ggcatcgatc actaaccctg ttttttcgtg gaaaaattct
    85921 tgatagcctt cattttttaa gttatctgca attccagctg actgtctgga ttgccaaaca
    85981 actgcatgat agattggacg tcctgtatct ttttcccata ttactgttgt ttcacgctgg
    86041 ttcgttatgc caatcccagc aatctgattt ggtttgatcc ctgattcgat gaacgctccg
    86101 gcaataactg actgtacaga attccagatt tcatttgcat catgttccac ccaactttct
    86161 tgcgggaaat attgcgtgaa ctctttttga gaactgccga tgtgtcttcc tttcttgtca
    86221 aagataattg ctcgtgagct agttgtacct tggtcaatcg ccatgatata cgttgattct
    86281 gtcattgtga ttccctccat tatatgcaaa cgctttcttt aactactctt atcatactac
    86341 tgttttaaaa aaagaaactg tcaaaatttt attctttttt tctttttttg acagtttttt
    86401 attatgaagc tattttttaa tcaattcttt tcttttttca gcgtctctat tcttcttttc
    86461 aatcgaataa tgtcgtaagg tgacgcaaat tctgacagat aatattcaca ctgttttttg
    86521 tttctctggc aaaatgtgat caccaaatca tattctttac cttcttcata acttccaatc
    86581 tcaataccgc cgattcccct cagttctctg atcagatatt gttgaaaagc aatacgataa
    86641 atcggcagac tttctaaatc gattccaatc actactggtt ttgcaatata aaaatcgatc
    86701 atcaaaagaa cattggcata ctggatcatc aggtaactaa gctcactatc ttcagctctt
    86761 ttcctcggca attgctgcac cgctgtttcc tgtaatttcg ccagtaactg tgttaaggaa
    86821 tgcccgagca ttttctgctg ctgttccaaa agccgatcac gctcatagac ttcgatcttt
    86881 ccaacaaaga aaaaccattc attgttgatc tgggaaattt ggtagcctac tgccttttct
    86941 tctttgattt tcaaacgacg aggacgataa tgccataaca tcgtttcccg aatgtaagta
    87001 tctaaaacag cggttggtaa ctttttgctc cgtgtcaaat catactgata aaatatttct
    87061 tcgtctacta tcgaaaaaga aacaaaaaag ctgtaaaaca tcattgtttc gtatacattc
    87121 agttctgctg ctgttcgact aagatgcatc acgatgattg gatcgattgc ttgataaagc
    87181 cggtccgact gatagagtaa tttcttttct ttcaaagttg cgtacgtact tttttcattc
    87241 aatagtcttt ttttcgtaat cccgagccag caagcaatct gtgcttctgc tgatgccgta
    87301 aacgttgttt ctaatacacg gtccaatcct agaatgaacg gtcttttttc tggtgtattt
    87361 tgcaaaaaaa gcggtcgctg atcttccggg atatattgga atagttcata ataaaaatat
    87421 cgtatctgta gctcttctcc gtataattga ccgtttttga tttgtaatcc aaattcctct
    87481 aagagctggt tcaattcttt gatctttcga aaaacagaag actcactgat attaaacgct
    87541 gtcgctaatc gaacgatgga tacttgctga tgttctaata aatagacgag catcttaaac
    87601 ttcaagcttt cctgcaataa aaacaataga attttttcca aacttacatc ttcgtccaac
    87661 acaagcagcg cctgatactc atttcgctgt acttctactt ttttttccat cattcggcca
    87721 atcaattcaa tatcttccaa atattgatca aacgaggctt ttgacagttg gacatgctct
    87781 ctcaaaccat tgatagaggc tgttccccct tgcagaacaa ggtttcgcaa aatcaggatc
    87841 tgtaactgct cttttctttc taataatgct tcaatcttca taggcagtat tcgccacctt
    87901 tgaccacacc atatctggca cacggtattc tggctggaaa aatagctcat gttcttctaa
    87961 ctgttcaaac gtccatcctt cttctactgc aattgtcagt tggtcaagaa ggtggaacat
    88021 ctcttttttg ctttggagct gtgctccaag aatctttttt tcagaatcaa atagaaattt
    88081 gagtttaact ttgctttgat ttattgctgt aagcttgtag tctacgtatc ctgtttcaat
    88141 tttttgggga taaaaaatgc tctcttcttc tgtcaaacct acgcttccta agaaatagcc
    88201 aaaccactga ttgccgactg gacgataggt tccctgatcc tttgtctgtg agccactaat
    88261 tgtcttgctg acatttcgtg ctgtacggac agcgttattt actagagaaa catacatttt
    88321 ttcatttgtt ggacgaaaag ttacttggat cgcatcgcca atcgcataaa cgtctttttg
    88381 agacgtttgc aagtagtcat ctacccagat cgttccatca tcattcaatg ttaattgttc
    88441 ttcccacatc tgattgtcgg gacgagaatg attagccagc aaaacaagat catagttctc
    88501 ctttcttttt tccgtctcta caatagcggt ttcattttcg gtcaaagaca caacttgttc
    88561 ttctaaaaaa agtgaaagcc cttgattttt catttccttt actaacggtt ctgtcatttc
    88621 aggatctaga taacgaaaaa gaattgactt tcggctttcg taaagatgga tttgtttttt
    88681 tcttgatgcc aacccttcag ctatttcgat tcctgcttgt cctgcaccaa taattgcaat
    88741 tttctttgca ttcatgatct tttgctgaag agccgttacg ttttgtatct ttttaactgg
    88801 gtgaataagc gttgattctg cagaaatatt tcgaaacttt tgatttgaac cagtcgctag
    88861 aatcaaacga tcaaaatcca atttttttcc tcctgctaaa acgacttttt tttctttcaa
    88921 tcctattact gtcttttctg tgtaaacatg gattcgatag cggtcagtca gttcttcttt
    88981 cgtgatccaa gaaagttgtt tttcactggt aacctttcct tttaatatca aattgaaccc
    89041 gctgggaatc catcctaact tcgattgctt ttcaatcaaa tatatttcac ttgttggcat
    89101 caattggcga agataaatag ccgcatgaat ccctgcaaac gacccgccga caattacaat
    89161 gttcatctgc attcctcttt cttataaggt gttctactgc taacttctca atcagaaaac
    89221 acagtattca taactcgctt tcaaccttat gatacatagc gatgcagcag aatcaaccct
    89281 ctccatcata ccgtgttcta ggtctgcttc tcaataatgt actagtggac ctaattgaag
    89341 agtatcattt ctgaagagat gtttcaaaaa atgtactctt ataaaagaaa aaaactcgcc
    89401 ttttacgtac ctctgtccaa ataggaaaca gaaaagaacg aaaagacgag ttttagcgtg
    89461 tgtaatgtaa tagttagaat agacacatca tttattttta attctttaat gcttcaacaa
    89521 ctgcttcgcc taattgatga gcagattgag gattttgccc tgtaagaagc cgcccatcaa
    89581 tttgaaccac ttctgaaaaa gctgctccag actgatagtt tgctcctttt tctttcaaag
    89641 catcttctgc taagaatggt acttcattcg ttgtaccatt tgcttcctct tcttcattac
    89701 tgaacccagt cactgtacgt ccagctaaaa tagattttcc agactcatct gtaactggca
    89761 acaatcctac aacaccgtga caaacagctg agaccagccc ttcatttcga tagatttgtg
    89821 aagcgacttt cccaagatct attgcttctg gaaaatccca cattgttccg tggccaccag
    89881 cataataaac cgctgcataa tcatttgcat ccacatcatc aggacgtaag gtatttccca
    89941 aggcccgatt tctgaaatct tcatcttcat aataggccca atcgatttca tccatattca
    90001 aaacactttg cggttctaaa ggtacatatc cgcctttagg actgacaaaa tctgcatcga
    90061 tcccattttt tactaataca tcataaaaat gagtcagttc acttaaccac aaacctgtag
    90121 gacgctcgat cttttcgtac ttttctacat ttgttaatac gatcaatact ttcattttcc
    90181 catcctcttt ctataaaaat tgcgttattg ataacgtgct ttcaagaaat tatataatgg
    90241 gcttggcaaa taaagctctt tatatgcttt ttcaaaaggt ttcgttacaa attcatcctc
    90301 acggttttcc aataatttct ctgcccagtc aggatcgacg atcatttgct gtccaattgc
    90361 tgcaagttct gcatatttca atacctgctc tacatcttcg cgattgcgga tcccaccgac
    90421 accaactaaa gggatctttc cttgaatcgt gttatggacc acttcaagaa tcgatttatc
    90481 gttgtattta tcagaacgag ctattcgatc atacatattt aatgaaacat gcagataatc
    90541 tagttttgac tcacgtaatc gttcaagaag ccagatcgtg tcttcaaaac ggatacctgg
    90601 tgtttcatat tcttctggtg agaaacgata ccctacgatg aatggttttt tagcatattg
    90661 ttcaaccgct gagaaaaccg ttgttaataa ttcttcaata aaacgataac gattttccaa
    90721 gctaccgccc cattgatcct ctcgtctatt cgaatgcgga gagaaaaatt gttggatcaa
    90781 atacgtattt gctccatgca actcgattcc atcaaatcct gattcaatcg ctctacgtgt
    90841 ggctttgcca aatgcttgga ttgtttcaac gatttcttct tcactcattg ctcgtggtgt
    90901 ttccgcatca ggacgttttg ctgcaactgc acttgcagat acaggctgtt ctccagataa
    90961 agttgctcga cttgtcattc gtccaccgtg gaaaatctga ataatcgctt ttgcgccttc
    91021 tccttggatt gctttcgcta gctcacttaa tcgaggaatc atttcatcat gagcgatgct
    91081 gagttcacct ggccagcctt ttccgatatc ttggacattt gcagcaccag taataacagc
    91141 accaagacct ttagaacgtt gtttataata agtgatctca tcttcggtga tcactccatt
    91201 gaaaaagctg agttgtgtcg tcataggcga cagcatcaat cggttcttca attgaactcc
    91261 tgaaggtaaa gtaattgatt gatcaaattg tgacattgtc attcctccca taattcgctc
    91321 tgattaacct atcgagaatc atctatcatt aaaccactcg attagttgta catgcaacta
    91381 atttgcttaa aaaattttat tttttttgta acttatctgt tgcatcattc attgtctggg
    91441 ctaattcttt acttgcttca tttcccaaaa ctgctttgta tgactgatga agttcttccc
    91501 agctaccatc gacttcctta gcaaattggc gtccttgttc tgtaagataa actaatgaca
    91561 agcgaccttc gctaactgtt ttgacgtatc ctttatattc taatttttca acaaaacgag
    91621 caatcgttga tggggcggaa tagagcattt gtgcaatatc tttttgtggt attccatctt
    91681 tttcttttaa tatcaataaa ataaaaccat aagttggtgc cagacctgtc cttctaaacg
    91741 cttcatcagc taatttattc aacatgcgat ctagcttttt cacgctaaag aaaagacatt
    91801 catctaacgc tttttcaatt ggcttttcca tttcatcccc tccattggcg tacatatcat
    91861 agtatgattt ttattttttg tcaaaaaaca cggaataatg tccgcctcct ctgtctagat
    91921 ttgaaaaaaa gaagtagcca tcttcaaaga atttcggctt gttttcgaca aaactccttc
    91981 tggaacaccg tgaatcagag gttgtgaaaa agtcgtttag ttttgagtat gaagaaaaat
    92041 tttgtgaaaa tggctttcca catttttgca taaatttggc ttaataccga aaaactggct
    92101 tttgaacacc gtttatatca gaggttgtga aaaagccgtc ctgcatcaag taataagaac
    92161 agccaaacaa aaatagttcc tcatattttg tcgttttttg agctaatgcc gatggtcagc
    92221 tgattgtaca ccgtttattg agaggtcgtg agcctgccgt tgaactccga gtcacaagga
    92281 gcaatttcaa aaaacagctt ctcatgtttt atgactatgg tgacttgtga tcgaggagtt
    92341 ggcaggagaa tactgtttat tcgtaaagaa ggggctgtga caagactttt gtcacagtcc
    92401 cttatttcct tttattatag gatttcgcca tttgattgga tgacttgtct gtaccaatca
    92461 aatgattttt tctttaaccg tttctttgtg ccattccctt cgtcatcttg atcaacatag
    92521 atatatccat aacgttttga catttgtgaa gtagaagcgc tgacgatatc aatgcagccc
    92581 cacgacgtat aaccaaataa atctactcca tctttgatag cttccttcat ctgctcaata
    92641 tgctgcttca aatagtcaat gcggtaatcg tcttgaacgg ttccatcttc ttctactttg
    92701 tctatagctc cgagtccgtt ttcagctatg aacagtggtt tttgatacct atcatataat
    92761 tgattcaaag caattctcaa acccttagga tcaatctgcc agccccattc acttgatgga
    92821 agatacggat ttttaattcc tccagttatt aagtttcctt ggaccccttc ccaattttct
    92881 ggatgagcac tgactgcact agacatgtag tagctaaaag aaacaaaatc aacagggtag
    92941 gctgctaaga tttcttcatc acctacttct atttttaagt tgacaccttt ttcttcaaac
    93001 attcgttgcg tatacgctgg ataatatcct cttgcttgaa catcactaaa gaacaaagaa
    93061 gttctttgct gctcaattgt tttttgaata tcatccggat cacaagaata cggaacaaga
    93121 agctgatagc tgatcataca accgacttgt gaacctttga taatttcatg acaagctttt
    93181 gtagctaaag cacttgctat aaactgatgg tgtgcacctt ggtatttcgc ttgttccaaa
    93241 tggggatggt tttcttcaat tactcctacg ctaacaaaag ggtgatgttt tacacagttg
    93301 atctcattga aagtcatcca atattttact ttatttttat agcgtttgaa tactgtttca
    93361 gcgaaacgaa cgtaaaactg aataagcttt cgattaggcc atccaccata ttctgtaacc
    93421 aaatataacg gcatttcata atgggaaaga gaaacgactg gctcaatccc atattttaga
    93481 cattcgtcaa acacttgatc ataaaaaaat aatcccgctt cattgggtgt ttcttcatca
    93541 ccatgaggaa aaattcttgt ccatgcgata gacatccgat agcatttaaa acccatttca
    93601 gcaaataaac ggatgtcttc tttaaagtga tgatagaaat catttccgtg tcttttagga
    93661 taataatact tgctgttggg atcctgcgcc gcttttactt gctgatgcgt catcatatgc
    93721 cattgatctg tccatttttc tttaggtaaa tcgctgtcga atgtatacac atcagacaca
    93781 gataatcctt tttcatctac atcccatgca ccttcacatt gattggccgc tgtcgctccg
    93841 ccccataaaa atccttctgg aaattttttc atatcgatcc ccctaaactt gctagttttc
    93901 ttgtttgatt atttttctta tgactagtaa tgatattttt gaatctacta ctatttgtaa
    93961 caataaccat tgtggtcgga tcatatccct ctttgattaa ttcttgcaaa tctacttcca
    94021 tcaataaatc gcctttacgt actttttttc catcagatac tgcactttga aagtatttgc
    94081 catttaaatt cactgtatca ataccgatat ggatcagtat ctctgctcct tcttcactga
    94141 ctaccccaat tgcatgtttc gttggaaaaa ctgttgaaac ttccccgttt acagaactgt
    94201 agatttttcc gtccgttgga atgatcgcaa acccttctcc taaagatcca ctagcaaatg
    94261 ttggatcgtt tacttcagat aaatcaatta tttcacctgc tgcaggagta cttaacacta
    94321 tatcctttga ggattcttct tcgatttcat ccttgtagcc gaaaagaaaa gttagcatac
    94381 aggaactgat catcgctaag aaataactag caactagcca aacaaatcct tcaccaatat
    94441 aaatcgggaa agacaaaata cttcttgtag caactgccaa agcaccggct cctgcggcac
    94501 cagctactcc accaaatata gctccacata tacaagcaat gtaaaatggt cgtttatatt
    94561 taagagtcac accataaaca gcaggttcag tgattccaaa taatgctgtg ataaatgcag
    94621 ataaagataa actttttact ttcttatttt tcgttttcaa aaatacccca agtactgctc
    94681 ctgcttgcgc agctacggca gtcatacatg ctggacctag cgtatcacga ccgtagaaag
    94741 aaagattgtt ggtcattact ggtacaagtc cccaatgtaa cccaaaaatc actaacactt
    94801 gccaaaatcc gccgataaca gcaccagata aaacagggct aaagttataa acagctaaat
    94861 atccagctgc aatcagctga cttgcataat ctgcaattgg acctacaact aataaaataa
    94921 ttgggaccat cacgatgata caaatgaatg gtgtcaataa atttcgaata ctttcgtgca
    94981 agcttttgcg aatcttaggc tcaatataag aaagaaccca tacagctaaa attgctggaa
    95041 taaccgtact tgtatacctt gctagaatca cgggcatccc tagaaatcgc aaggttatgc
    95101 tatcactata tgcactgaca atcgtaggat aaacgagagc tgctgcaata accatagcga
    95161 tgaaacgatc tgtattgaac ttttttgcag cagtatatgc caaaaatata ggcaagaaat
    95221 agaaaaatgc atcagctgct gcattcaaaa tgatataagt tcctgattca gtagttaata
    95281 aattcaaact cgtacataag gcgagtactc ctttgatcaa accacttcct gccaagacgc
    95341 ctaaagtcgg ggcaaaaaca gcggcgatca tgtctataaa agagtttaaa atgccttttt
    95401 tctcagttcc tctctttcca ctagatgcat catcttctag tttcatttgt ttggataata
    95461 cgtcatacac ttcatttact gcatttccta ctacgacttg aaacattcct ccagcttcga
    95521 ctacagtgat cacaccttct agttgctcta ccttctcctt atctgccttc ttattatctt
    95581 ttagggtaaa gcgtaacctt gttgcacaat ggtaaacatt gctgacattt tcttcgcctc
    95641 cgacaaatcg gacaatatct ttcgctaatt gtgaatagtc catttttact tcccccttgt
    95701 taattaatgc cgtagcaatc cttcttacaa gataagtata gttcttagaa aacggtttct
    95761 ccattcaatt agtacccctt ctaatagcga acgaaactgt acaatagtgt gtatggttta
    95821 cttagccgag caaatcactc actgcttgaa agaaaacaag tatatcgttt ctcttatagt
    95881 caaaggagac aaaaaaaaca cgcttgatct tcctttgctg atgtataagt ggtatctcaa
    95941 gctgatactc tgtgattttt gtttctactt ggcttttgat aagacagcac aacaaagtct
    96001 cggcatttaa cggaagcata actaggtcag aactaggaga gattagttgc tgaatggaat
    96061 ctatcttttt atggattttt atttgatttt cttctttgta gtaatgatca agcagttttg
    96121 ttttcttttt ttcttcaact atggtcagct tttcacgcaa aatctgataa atctcccaag
    96181 attcaaactc atctagaaca ctaggtagat aaatcaaatc atcccactca aaaataagct
    96241 cttgctcagt agtcaaaatt gcatcatatt ccgttatttt gtttgattga tttttgaaaa
    96301 gatacactgg ttctatccgg cattctttga tccacatgtt catactttgt tgaatctgtt
    96361 tttttagtgt attcaaaata ctaattggct ggttactaac aatcagtatc tccccttgtt
    96421 ttcgaaaagg attcaacgct tcggcaagat acaaaacaaa ataagcgctt tcagctaaat
    96481 tgggacgtaa ttgaaaatgt attgggaaga aacgccgaat taaataaatt tctaaaggat
    96541 aacgggagat gatcttttgt acaaaatgat tcatggtatt atgtccggca tccatcctcg
    96601 ttttcatttg tttgatatgg gttagcaaaa attctgtttc atggaacaat aacgaaacag
    96661 gaagatctaa aaaacgaata atcgtttgct caaatgctct tagattcttc tctatttctt
    96721 tgtttttttc atcattaatc atcaaataat ttaactctaa tagtcgattt tcaattgctt
    96781 gtttttcttg attggaaaaa gaatagggta gttctgatga taactttttt gtgagggtct
    96841 ctaccatcgg aataaataat gtatacctgt ttgttttttc taaaagttct tctcttcgaa
    96901 gaatggtcaa aacgagatac cgagaaaatc gtatgaagtc ctctcctgaa acaatataat
    96961 tatatgatat aagaacctct tttaatgtct tttggacagt cggcatgata ttggaaaaaa
    97021 ttgtactgat ttgttgagga aaccttgcgt gttggatcac cgtttctgtt ccaacaagag
    97081 ataagaaaat tctttttgaa ttttcttcag caatgatttt tagtcctttt gtcgacgaaa
    97141 cttccagttc taatttagga ttgcgctcga tccaacgcaa aatcgttttt atttgtaatg
    97201 atacggctgt ttttgaaaga aagaaaatgt ctgctatctt ttgcatactg acataccctt
    97261 tttgaaacaa taagtacgtc acaatagttg aagctcggaa atccatgctg gaatagattt
    97321 tatgtttcgt cacctcttgt atgatttgct gtttagtttc agattcaatt gattctaaaa
    97381 aataacctga acgtttattt gactggataa tgattttctt ctcgtagata gcaagcatat
    97441 ttttgatttc agtttggatc gttttcttgt tggtacctaa agcttcagct aattcttcgc
    97501 ttgttatcca atgattctta tttcttaata ctacaagaat ttggagttgt cgtttattta
    97561 agtccatttg cttacac
//
LOCUS       DACDIH010000002        94816 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDIH010000002 DACDIH010000000
VERSION     DACDIH010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 94816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 94816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..94816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.002"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(53..2122)
                     /gene="pknB"
                     /locus_tag="IV281_000083"
     CDS             complement(53..2122)
                     /gene="pknB"
                     /locus_tag="IV281_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP8779132.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(2119..2859)
                     /locus_tag="IV281_000084"
     CDS             complement(2119..2859)
                     /locus_tag="IV281_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP8779133.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(2872..4230)
                     /gene="rsmB"
                     /locus_tag="IV281_000085"
     CDS             complement(2872..4230)
                     /gene="rsmB"
                     /locus_tag="IV281_000085"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP8779134.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(4230..5168)
                     /locus_tag="IV281_000086"
     CDS             complement(4230..5168)
                     /locus_tag="IV281_000086"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP8779135.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(5161..5652)
                     /gene="def"
                     /locus_tag="IV281_000087"
     CDS             complement(5161..5652)
                     /gene="def"
                     /locus_tag="IV281_000087"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8779136.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(5667..8075)
                     /gene="priA"
                     /locus_tag="IV281_000088"
     CDS             complement(5667..8075)
                     /gene="priA"
                     /locus_tag="IV281_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP8779137.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(8328..8633)
                     /locus_tag="IV281_000089"
     CDS             complement(8328..8633)
                     /locus_tag="IV281_000089"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8779138.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(8638..9252)
                     /gene="gmk"
                     /locus_tag="IV281_000090"
     CDS             complement(8638..9252)
                     /gene="gmk"
                     /locus_tag="IV281_000090"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP8779139.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(9739..10023)
                     /locus_tag="IV281_000091"
     CDS             complement(9739..10023)
                     /locus_tag="IV281_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779140.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(10041..10931)
                     /locus_tag="IV281_000092"
     CDS             complement(10041..10931)
                     /locus_tag="IV281_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP8779141.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(10951..11169)
                     /locus_tag="IV281_000093"
     CDS             complement(10951..11169)
                     /locus_tag="IV281_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779142.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(11139..12839)
                     /locus_tag="IV281_000094"
     CDS             complement(11139..12839)
                     /locus_tag="IV281_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP8779143.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            13105..13878
                     /locus_tag="IV281_000095"
     CDS             13105..13878
                     /locus_tag="IV281_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779144.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(13959..14528)
                     /gene="thiT"
                     /locus_tag="IV281_000096"
     CDS             complement(13959..14528)
                     /gene="thiT"
                     /locus_tag="IV281_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP8779145.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(14594..14696)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(14702..15673)
                     /locus_tag="IV281_000097"
     CDS             complement(14702..15673)
                     /locus_tag="IV281_000097"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8779146.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(15767..17140)
                     /gene="glmU"
                     /locus_tag="IV281_000098"
     CDS             complement(15767..17140)
                     /gene="glmU"
                     /locus_tag="IV281_000098"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP8779147.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(17643..18461)
                     /gene="purR"
                     /locus_tag="IV281_000099"
     CDS             complement(17643..18461)
                     /gene="purR"
                     /locus_tag="IV281_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP8779148.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(18628..19452)
                     /locus_tag="IV281_000100"
     CDS             complement(18628..19452)
                     /locus_tag="IV281_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8779149.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(19425..20105)
                     /locus_tag="IV281_000101"
     CDS             complement(19425..20105)
                     /locus_tag="IV281_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8779150.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(20128..21066)
                     /locus_tag="IV281_000102"
     CDS             complement(20128..21066)
                     /locus_tag="IV281_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP8779151.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(21311..22162)
                     /locus_tag="IV281_000103"
     CDS             complement(21311..22162)
                     /locus_tag="IV281_000103"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP8779152.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(22316..24889)
                     /gene="mprF"
                     /locus_tag="IV281_000104"
     CDS             complement(22316..24889)
                     /gene="mprF"
                     /locus_tag="IV281_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8779153.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            25030..25836
                     /gene="proC"
                     /locus_tag="IV281_000105"
     CDS             25030..25836
                     /gene="proC"
                     /locus_tag="IV281_000105"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP8779154.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(25915..27471)
                     /gene="rny"
                     /locus_tag="IV281_000106"
     CDS             complement(25915..27471)
                     /gene="rny"
                     /locus_tag="IV281_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP8779155.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(27882..28931)
                     /gene="recA"
                     /locus_tag="IV281_000107"
     CDS             complement(27882..28931)
                     /gene="recA"
                     /locus_tag="IV281_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP8779156.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(29040..30281)
                     /locus_tag="IV281_000108"
     CDS             complement(29040..30281)
                     /locus_tag="IV281_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP8779157.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(30521..31099)
                     /gene="pgsA"
                     /locus_tag="IV281_000109"
     CDS             complement(30521..31099)
                     /gene="pgsA"
                     /locus_tag="IV281_000109"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP8779158.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(31308..32192)
                     /locus_tag="IV281_000110"
     CDS             complement(31308..32192)
                     /locus_tag="IV281_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8779159.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(32970..34256)
                     /locus_tag="IV281_000111"
     CDS             complement(32970..34256)
                     /locus_tag="IV281_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8779160.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(34249..35517)
                     /locus_tag="IV281_000112"
     CDS             complement(34249..35517)
                     /locus_tag="IV281_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8779161.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            35769..36230
                     /gene="mscL"
                     /locus_tag="IV281_000113"
     CDS             35769..36230
                     /gene="mscL"
                     /locus_tag="IV281_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP8779162.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(36289..36735)
                     /locus_tag="IV281_000114"
     CDS             complement(36289..36735)
                     /locus_tag="IV281_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8779163.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(36828..37073)
                     /locus_tag="IV281_000115"
     CDS             complement(36828..37073)
                     /locus_tag="IV281_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779164.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(37188..37766)
                     /locus_tag="IV281_000116"
     CDS             complement(37188..37766)
                     /locus_tag="IV281_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8779165.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(37843..38373)
                     /locus_tag="IV281_000117"
     CDS             complement(37843..38373)
                     /locus_tag="IV281_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP8779166.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(38377..39237)
                     /gene="rlmB"
                     /locus_tag="IV281_000118"
     CDS             complement(38377..39237)
                     /gene="rlmB"
                     /locus_tag="IV281_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP8779167.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(39230..39625)
                     /locus_tag="IV281_000119"
     CDS             complement(39230..39625)
                     /locus_tag="IV281_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8779168.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(39622..41034)
                     /locus_tag="IV281_000120"
     CDS             complement(39622..41034)
                     /locus_tag="IV281_000120"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP8779169.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(41031..41567)
                     /gene="cysE"
                     /locus_tag="IV281_000121"
     CDS             complement(41031..41567)
                     /gene="cysE"
                     /locus_tag="IV281_000121"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP8779170.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(41889..43349)
                     /locus_tag="IV281_000122"
     CDS             complement(41889..43349)
                     /locus_tag="IV281_000122"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP8779171.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(43519..44676)
                     /locus_tag="IV281_000123"
     CDS             complement(43519..44676)
                     /locus_tag="IV281_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP8779172.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(44710..46080)
                     /gene="radA"
                     /locus_tag="IV281_000124"
     CDS             complement(44710..46080)
                     /gene="radA"
                     /locus_tag="IV281_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP8779173.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(46143..46670)
                     /locus_tag="IV281_000125"
     CDS             complement(46143..46670)
                     /locus_tag="IV281_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP8779174.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            46900..47706
                     /gene="proB"
                     /locus_tag="IV281_000126"
     CDS             46900..47706
                     /gene="proB"
                     /locus_tag="IV281_000126"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP8779175.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            47703..48950
                     /locus_tag="IV281_000127"
     CDS             47703..48950
                     /locus_tag="IV281_000127"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP8779176.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            49026..49370
                     /locus_tag="IV281_000128"
     CDS             49026..49370
                     /locus_tag="IV281_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779177.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(49487..49813)
                     /locus_tag="IV281_000129"
     CDS             complement(49487..49813)
                     /locus_tag="IV281_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779178.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(50186..51387)
                     /locus_tag="IV281_000130"
                     /pseudo
     CDS             complement(50186..51387)
                     /locus_tag="IV281_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            51504..52019
                     /locus_tag="IV281_000131"
     CDS             51504..52019
                     /locus_tag="IV281_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8779179.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(52218..52664)
                     /locus_tag="IV281_000132"
     CDS             complement(52218..52664)
                     /locus_tag="IV281_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8779180.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(52711..54189)
                     /gene="msr"
                     /locus_tag="IV281_000133"
     CDS             complement(52711..54189)
                     /gene="msr"
                     /locus_tag="IV281_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8779181.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(54543..55172)
                     /locus_tag="IV281_000134"
     CDS             complement(54543..55172)
                     /locus_tag="IV281_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8779182.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(55156..55620)
                     /locus_tag="IV281_000135"
     CDS             complement(55156..55620)
                     /locus_tag="IV281_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8779183.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(56107..57375)
                     /locus_tag="IV281_000136"
     CDS             complement(56107..57375)
                     /locus_tag="IV281_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP8779184.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(57606..58343)
                     /locus_tag="IV281_000137"
     CDS             complement(57606..58343)
                     /locus_tag="IV281_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8779185.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(58410..58979)
                     /locus_tag="IV281_000138"
     CDS             complement(58410..58979)
                     /locus_tag="IV281_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP8779186.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            59107..59979
                     /locus_tag="IV281_000139"
     CDS             59107..59979
                     /locus_tag="IV281_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8779187.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            60321..61193
                     /locus_tag="IV281_000140"
     CDS             60321..61193
                     /locus_tag="IV281_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8779188.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            61190..63148
                     /locus_tag="IV281_000141"
     CDS             61190..63148
                     /locus_tag="IV281_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8779189.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(63399..64250)
                     /locus_tag="IV281_000142"
     CDS             complement(63399..64250)
                     /locus_tag="IV281_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8779190.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            64373..64840
                     /locus_tag="IV281_000143"
     CDS             64373..64840
                     /locus_tag="IV281_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP8779191.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(65103..65585)
                     /locus_tag="IV281_000144"
     CDS             complement(65103..65585)
                     /locus_tag="IV281_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8779192.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(65685..66548)
                     /locus_tag="IV281_000145"
     CDS             complement(65685..66548)
                     /locus_tag="IV281_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8779193.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            67146..67994
                     /locus_tag="IV281_000146"
     CDS             67146..67994
                     /locus_tag="IV281_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779194.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(68165..68506)
                     /locus_tag="IV281_000147"
     CDS             complement(68165..68506)
                     /locus_tag="IV281_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAP8779195.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            68592..69299
                     /locus_tag="IV281_000148"
     CDS             68592..69299
                     /locus_tag="IV281_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP8779196.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            69286..69609
                     /locus_tag="IV281_000149"
     CDS             69286..69609
                     /locus_tag="IV281_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8779197.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(69698..70735)
                     /locus_tag="IV281_000150"
     CDS             complement(69698..70735)
                     /locus_tag="IV281_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8779198.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(71182..72165)
                     /locus_tag="IV281_000151"
     CDS             complement(71182..72165)
                     /locus_tag="IV281_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP8779199.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(72581..73351)
                     /locus_tag="IV281_000152"
     CDS             complement(72581..73351)
                     /locus_tag="IV281_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8779200.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(73344..74279)
                     /locus_tag="IV281_000153"
     CDS             complement(73344..74279)
                     /locus_tag="IV281_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8779201.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(74873..76522)
                     /locus_tag="IV281_000154"
     CDS             complement(74873..76522)
                     /locus_tag="IV281_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8779202.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(76717..77613)
                     /locus_tag="IV281_000155"
     CDS             complement(76717..77613)
                     /locus_tag="IV281_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8779203.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(77606..78286)
                     /locus_tag="IV281_000156"
     CDS             complement(77606..78286)
                     /locus_tag="IV281_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8779204.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(78287..79069)
                     /locus_tag="IV281_000157"
     CDS             complement(78287..79069)
                     /locus_tag="IV281_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8779205.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(79062..79979)
                     /locus_tag="IV281_000158"
     CDS             complement(79062..79979)
                     /locus_tag="IV281_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8779206.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(79982..80266)
                     /locus_tag="IV281_000159"
     CDS             complement(79982..80266)
                     /locus_tag="IV281_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8779207.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            81054..84281
                     /gene="fss3"
                     /locus_tag="IV281_000160"
     CDS             81054..84281
                     /gene="fss3"
                     /locus_tag="IV281_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP8779208.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            84393..84707
                     /locus_tag="IV281_000161"
     CDS             84393..84707
                     /locus_tag="IV281_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8779209.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            84720..85094
                     /locus_tag="IV281_000162"
     CDS             84720..85094
                     /locus_tag="IV281_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8779210.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            85104..85448
                     /locus_tag="IV281_000163"
     CDS             85104..85448
                     /locus_tag="IV281_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP8779211.1"
                     /translation="MEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQGLL
                     EVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFLKQL
                     VETKQLPVVVND"
     gene            85517..86533
                     /locus_tag="IV281_000164"
     CDS             85517..86533
                     /locus_tag="IV281_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8779212.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLSALFIGWMGVFYFPELRQFGL
                     SKVSFSTIEIAWSMKDLVCLLGSLALSGSMMLLYIHFFPDHWRSLWNRQKLARMILEN
                     HWYEVKQTQSDGFFKDLNRSRTKETISYFPKVYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISKCVEDFYERMMARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            87127..89001
                     /gene="ltrA"
                     /locus_tag="IV281_000165"
     CDS             87127..89001
                     /gene="ltrA"
                     /locus_tag="IV281_000165"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8779213.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            89127..89483
                     /locus_tag="IV281_000166"
     CDS             89127..89483
                     /locus_tag="IV281_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP8779214.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            89528..90670
                     /gene="dcm"
                     /locus_tag="IV281_000167"
     CDS             89528..90670
                     /gene="dcm"
                     /locus_tag="IV281_000167"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP8779215.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            90660..90949
                     /locus_tag="IV281_000168"
                     /pseudo
     CDS             90660..90949
                     /locus_tag="IV281_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            91205..92389
                     /locus_tag="IV281_000169"
     CDS             91205..92389
                     /locus_tag="IV281_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8779216.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            92404..92499
                     /locus_tag="IV281_000170"
     CDS             92404..92499
                     /locus_tag="IV281_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP8779217.1"
                     /translation="MEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
     gene            92528..92749
                     /locus_tag="IV281_000171"
     CDS             92528..92749
                     /locus_tag="IV281_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779218.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            92762..93265
                     /locus_tag="IV281_000172"
     CDS             92762..93265
                     /locus_tag="IV281_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8779219.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            93392..94429
                     /locus_tag="IV281_000173"
     CDS             93392..94429
                     /locus_tag="IV281_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779220.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            94444..>94816
                     /locus_tag="IV281_000174"
     CDS             94444..>94816
                     /locus_tag="IV281_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HAP8779221.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDK"
BASE COUNT        27592 a        19660 c        17471 g        30093 t
ORIGIN      
        1 agaatgcaac aaatcaatgt cgcattccct tctgatatat tttattcagc acttattttg
       61 aactttctga ggtgctagct gatgaacttg tggagctgga agattcttct tttgaagagt
      121 tgcttgtgct actggagcta cttgagctgc tagaactgtc tgaagagtct ttttcagaac
      181 tagaagacgc tggatctggt cctttagagt aaattacgtt cacaaccatt cctttggtaa
      241 ttgtcgttcc tgcccctggt actgtacgaa tcactttatc tttatccacc gtgtttgaat
      301 attcggaagt ctcatgcccg atatattctg caccgacact agctaaataa ctttgtgctt
      361 cgctttttgt atagccggag atatcattca atttaggctc tgttccttgg cttacgacaa
      421 aattgatgta agtttcttct gctacaactt cacccgaggc tggatcctga ctgatgatcg
      481 aacctgatgg tacagtgtcg ctatagtcat aagtagcact aatattattg atattgaatc
      541 cttgtgtaac aagccaatta cgaacagttt cgtagttttc tcctacatga ctactcaaag
      601 tcactgtttc tggtcctttg ctgactgtga aagtaatcgt gtctgtttta ggatcgactt
      661 ctgtatcttc tgatggagat tgcgaaatga tcattccgct ttctacctct gaactgaatt
      721 cctcttcttt ttgaatcaag tcagcagaaa accctaattc tttcaaactt gcttcggcat
      781 cttcataact tttaccactg taatcatcta atttgatttt tttcgtgccg ctactaatat
      841 acagagtgac ttcgcgtttt tgcttgactg tcgttcccgc tgccgggtct gtcttcacta
      901 cattaccttc ttcgattttg tcatctgcaa tttcttctgt ttttgttgct acttctaatt
      961 tcgcttttgc gagtgtttct ctagcagaag cttcagaaag atcagaaaca tcaggaacaa
     1021 caacttctcc tcgcccgcca gaagcaaaga atacagctgt tccgattcct aaaacagcaa
     1081 gaatagctaa taggatcagc caccattttt tctgtccttt ctttttagga tcaggcagaa
     1141 cttcttcctc ctctggtaag gtttcgtttt ctggcttgtc ttttggcaga ggcatagatt
     1201 tgaatgaatc aggcattggt gcatcttccg taaggggtgt gataactttt gtttcattat
     1261 ccatcacatg aggctgccat ttttcttcgt ttgcacgagc tggtgataat actgtagaaa
     1321 gatcatcaga catttcctcg gctgacttat aacggtctgc aggttcttta gccgttgccc
     1381 gtaaaatcac gttttccagt gcttgtggca catttggatc aagtgctttt atagatggaa
     1441 gatcatcttg aaaatgtttt aaagcaattg ttacagcaga ttcaccatca aacggtacgc
     1501 tacccgtcaa catctcataa aggatgatcc ctaaagcata aatatctgac tgcttcgttg
     1561 ccatacttcc tctggcctgc tcaggggaca gataatgaac agatcctagc atcgtattcg
     1621 tctgcgtgat cgatgtttca gataaagcga tagcaatccc gaaatccgtg atttttacaa
     1681 caccttcatt atcgatcagt acgttttggg gtttcaaatc tcggtggatg atttgatggt
     1741 tatgagctaa agaaatagca gaaaggatct gctgcataat atttactgct gtttcataag
     1801 gaacaggata atgtgtttgg atatatcgtt tcaagtccat tcctttgacg tattccatca
     1861 ccagatactg catgttgtct tcttcaccaa catcgtaaac actaacaatg tttggatgaa
     1921 cgagttctgt tgctgctaaa gcttctctct gaaatcgtcg aatcgcagtc tggtcatttt
     1981 gaaagtcaaa tcgtaagact ttaactgcta catcacgatc caagattaaa tcgtgcgcca
     2041 aaaatacatt tgccatacca ccgctaccga tatttccagt gatttgataa cggccattta
     2101 attttttgcc caactcgatc atacactgtc ctccttatat tcgataagaa gaacggtgat
     2161 attatccgct ccgcctgctt cattggcacg atcaatcagc tcagttactt tatgagacaa
     2221 tgggccttct tgattgatga tcgttcctat ttcttcttca gacagcatgt tcgtcaagcc
     2281 atctgaacat aacagcagtc gatcttttag ttgccaaaga tgagtagaaa catctacttc
     2341 gactgttcca ggcatgccga cagaacgtgt taaaatattt ttccttggat ggttcactgc
     2401 catttcttcg ctgatttctc ctgatttgac tagttcattg actaaagaat gatcctccgt
     2461 caattgcatg attttatctt cacgaatcaa ataagctctg ctatccccaa cattggcaat
     2521 cgtgaaccag tcatctgaca gagcggccgc tacgattgtt gttcccatac cgttatattc
     2581 cggctgttct tgtccacgct gataaatcag catattttct tcttgaatcg attgaatgaa
     2641 ccattgggcg attttttcat cgtcaacagc ttcttgtttt tcccaagctt cgcctagatt
     2701 cgttaccgcc atctgactgg caatgtctcc ggcacgatga ccacccatcc cgtctgctaa
     2761 tacagcaagc ttgagacctg aacggctggt aaatacactg gcatagtctt gatttgtgtt
     2821 acgtcgtctt cctacatctg attgatattc aatctgcatt ttttcacctc attacttttt
     2881 gcgcaaacaa caaatgaaaa atccatctgt gtaaaactga tgcggataca aagtcaacat
     2941 tttctcttcg attgatgatt gtagcatagg attggtcaaa acatcaatcc tctcaaaatc
     3001 agggtgtctt tccaagaaag cagaaacaac gtcttggttt tcttctttta agatcgtaca
     3061 agtgctataa gtcagaatac cggaagactt taaagttggt gcgcagcttt ctaaaatttc
     3121 caattggatt tttggtaaat tcgctaattc atccggctgt tttttataac gaatatctgg
     3181 ttttcgccga agcagcccaa gacctgaaca aggcgcgtct actaaaatcc gatcaaactg
     3241 ctctgcttcg aactgtttac tgacttctct agcatccatc ttttgagcgt agacagcaga
     3301 agagacacct agacgtgctg cattatcttc aatgagtttg actttgtgtt cgtgtacatc
     3361 taaagcgacc acttctccac cgcatgacgg atctaaaaaa gaagcaatat gcgtcgtctt
     3421 acctccgggt gctgcacagg catctaagac atgatgttca ggctcgattt gcatagacgg
     3481 cgctacaagc atcgaacttt catcttggat cgtcatgacg ccatcgtgga acaatgagct
     3541 agaagctaaa tgcccttttt tcgctactat accgtatgga ctgacttggc ttttttcagc
     3601 tgtgatctct tcttcttgca aaacagtgat ggcctgatct ctggaaagtc ggcgtgtatc
     3661 tactcgggca ctagcatggc taggctgata tagcgataaa ccgagctttt cagtttcttc
     3721 gtatccgatt tgatccacca gtcgtttagt catccataga ggcaggctga ttttgacaga
     3781 aagtctttcg ataggatcag caatgccttg cagatctggt gctccttgac gctgataatt
     3841 cctcaagaca ccatttacaa atttccctgt cccaggattt ccttttactt ttgcgatttc
     3901 gaccgcctcg tttaaaatag catgtgcagg gactttatct aaaaattcta attggtatac
     3961 agaaagataa agaagcgtct tgacccagtc atccactttt tttgctttag cgatcaatgg
     4021 tgcaacgtaa aattccaata gtcgctgatg gctaatcgta ccatagatca attctgtaaa
     4081 taaacccaca tctttttgat ttagctcgct ttgtttgatc atctcattca acaaaagatt
     4141 cgagtaggcg ccacccttgt tcactcgctc taatgtcagt aatgccatat atcgtactga
     4201 ttttttcatt tttttaggga tttttttcat tatttcacct gctgaccaat ctgaacttgc
     4261 tgaccgcttc cgttaaggaa atcatgtacg gcctgttttc ctttaccagc tggctgtaat
     4321 tcattgatgg ctaagacggt ctgtttaccg caagcaatcc atagattttt tttgtctttc
     4381 cggatgatcg taccaggtgc tttgtctgta gtttctgcca atacttcaac cgataataat
     4441 ttccagcgta tgccctcata ggtcgtgaat gcaattggcc aaggacgcat tccgcgtact
     4501 tggttgtcaa tttcttctgc agttttatgc caatcgattg cttcttgttc ccgtgtgatg
     4561 tttggcgaaa aagttgcttc tgactcgtct tgaggtactg gtttttgtcc atccaaaata
     4621 ttgggcaatg tttctagcaa tagctttctt cctaaaaggc taagcttttc aaacatcgtc
     4681 cctacatcat cttgtttagt gattggtatg ctttcttgcg catagattcc gcctgcatcc
     4741 atttttttga tcatttccat gatcgttaca ccagtttctt tctccccatt catgatggca
     4801 taatgaacag gcgcacctcc ccgatattta ggaagtaaag aagcatggac attgattgcc
     4861 cctaattttg gtacttgcaa taacttttca ggcaagaatt ggccaaatgc cgcagtgacg
     4921 atcacatcag gcaccaattc aatgatttct tccatttctg gcgaaccgga gattttctct
     4981 ggctgcagca cacggatacc gtgcttcaga gccgcttctt ttaccggtgt cggcgtaatt
     5041 actcgtttcc gcccaactgg acggtcgggc tgtgttacca ctgcctggat ctcgtaacct
     5101 gattctatca gcccttcaag aatcggcaca gaaaaagcag gtgtgcccat aaatactagc
     5161 ttagtcatca agatgttcct ccatatattc ttcaagttct tcttccggga tctgctgtat
     5221 cattttctca atgaacaaga taccgtctaa gtgatcgatt tcatgctgga aggcacgggc
     5281 taaatagcca aaagccgtca cttctatctc ttctccgtct cgatcataat aacgcacggt
     5341 cacttcatct gctcgcttga ccgttccata gacatgcgga atactgagac aaccttctac
     5401 gtcaaggctt tcaccttttg cttcgatgat ttctgggtta atcagttcaa atttctcgcc
     5461 ttcatctact tcgatcacag caattctctt gttttgtcct acttgaggag cagcgatccc
     5521 tattccgtca ttcgcaatca ttgtctcata taaattgtct aataaagcga ttgtctcgtc
     5581 cgtgattaca tcgattggct gagcagttct ttttaattta tcattagggt ggatcaatat
     5641 aggatagcgc ataatttggc cctttcttaa ataaagttta atggttcgtt gtcaattgcc
     5701 acaaaaagac cttgtcgctg ttctttctgg cttccattca aaatttcatc caaaacatga
     5761 ggaagattcg gttcatgttt atatttgatg attagttgat aataatatcg gtttttcact
     5821 ctcaaaatca tgctgggcgt tggccctaat agcaagcttt gagggcttaa cacttctttt
     5881 agctgattgg caatctgaaa gattttcttt gccgctactt gttcttcagg atggctggcg
     5941 gttattttca cagtaaagta ataaggagga tagcctccac gatgccgtac atgcatttcc
     6001 tgctgataaa actgctcata attttgtttt ttagccaatt ctatggcgta atgttgagga
     6061 ttgaatgttt ggatcacgac ttcgcctgct ttttccgctc gccctgctcg tcctgaaact
     6121 tgggtaagca attggaatgt atgctcacta gaacgaaaat ccggtaaatt cagggaggta
     6181 tcagcattca acaccccaac taatgtcact tctggaaagt ccaaaccttt agcaatcatt
     6241 tgtgtcccta ataatatatc tgcttctttt gcaccgaatt tttgtaaaat ctgttcgtgt
     6301 gccccttttc ttcgagtggt atctacatcc atacgcaaaa tcctagcttg cggataaagt
     6361 tctcggagtt cctcttctac tttttgagta cctgttccat agtagcggat tttattgccg
     6421 ccacaattag gacagcgatt gggaataggt tcttcatgtc cgcaataatg gcagcgcatt
     6481 gaacgtgtat ccatatgcag tgtaagagag atatcgcaat taggacatgg aagaacaaat
     6541 ccacagtccc tacacatcac aaaagacgaa tatcctcgac gattgagcag taggacagtt
     6601 tgttcttttt tgtctaatcg attttgtatt ttttcctgca aattagcaga aaaagtagag
     6661 gtccgatgat tctgaaactc ttcacgcata tctacgatct caatagctgg aagctgtgca
     6721 gctgctttgg ctcggtggtt taactgcaat aactgataaa cgcctttttg agctcgtgct
     6781 cttgattcta aagagggcgt cgcgcttcct agaactattg ggcaatgatg atatttactg
     6841 cgccaaattg ctagatctct cgcatgataa cgcggtgtct catcttgttt gtaacttgat
     6901 tcatgttctt catcgataat gatcagcccg atatttttca gcggggcaaa aatagcagat
     6961 cgtgcgccta ctactacttg cgcttcttcc cgctcaatct tgcgccattc atcatatttt
     7021 tcaccttggg aaagtccgct atgtaagacc gcaactgatt ctccgaagcg tcctttgaat
     7081 cgctcgacca tttgcggagt caatgcgatt tcaggcacta acataatagc cgttttattt
     7141 tctgacaaaa catcagcgat tgcctgaaga tatacttctg ttttcccgct gccagtgatt
     7201 ccctccagca aaaagacctg atctttttgc tgctgacctg ctgtgatgat ttgttcgaca
     7261 gcattttttt gttctgcatt cagttccaat gccgtcgttt ggtcaaatac tcggtcttta
     7321 taagggtcgc gataacgttc tgattctata aactccagcc agcccttttc ttttccttga
     7381 ttcaagacag cagtactgat tcctttattt ttgaaataag caattggttc ttcttggctc
     7441 agttcagata aacacaaaag aagttctttt tgtttcttgg cattttgccg taattttgcc
     7501 cattcttctt cgatttgctt ttcagttaac aaagaacgga ccaaacgaat cgtttttact
     7561 ttatttcgtg tatggacttc atatttgata tctactaatt gctcttggcg ccagcgaatc
     7621 aattgagaaa ggctgccttc ttcttctgct tccttccagg aaatcacttc tcgctgatca
     7681 aacaatacct gaacttctgg tgtgtctgcc aaaggataga tcaatttatc atattctgct
     7741 ttcatcacac tcggaagcat cgtttgtaga caagtgattt tgaatgcgta agtaattttt
     7801 ttcatataat cagctaatga aagtaattct tcattgacca ccggagcaag gtctagcaaa
     7861 cgaataactg ccttcaaaga tggattagct gattcttctg atttttggat agccataaca
     7921 aatccttgga catgacgatt gccattacca aaagggactt ctactcgcat cccgacttgc
     7981 aaagctgttt ccatttcaga gggaaccaaa taagtgaatg gctgatcggt ctgcatagta
     8041 ggtacgtcca caatgacttc agctgttaat gtcatggttc gtttcctcct ttttttgtct
     8101 tttcttatca agcgacaaaa agttccttgc ctattttact agagatcgat ggatttgtct
     8161 tatcttctgc caaatcctaa caaaaaatta acagcagata agaaaaaaaa gaactgtgtc
     8221 aaaaagttca gacacagcct tttctaccag acaataagtg gcgaatgttt gaaaaggaaa
     8281 cagctgggtt tgcttttctt tttagatgtt cgctctattt ttctttatta cattttttgc
     8341 tcttcacgga tacgtgattc caattcgtgt tgttcacgct ctttttgtgc ttgacgttcc
     8401 tcttcttcca tcttcaaacg ttcacgtttt aattcaggat gtggatcatt gactacattt
     8461 cctgcatcga tttcttctaa agcttgaccg acgcttttta ctgaatcaaa agaatctaga
     8521 gtaggctgtg cacttgcatc caattcatgc gcgcgtttac ttgctaaaat cactagagaa
     8581 tattttgaat ttacacgatc taatagtgag tcaattgaag gttttaacat cataagacta
     8641 catctccttt aacattttaa tatatttacc gattacgcga tctactcgaa aatgttcact
     8701 ggcaataatg tttttgattc gttcaactgc tagcggcact tcatcattta caacagcata
     8761 gtcgtaaaga gccatcattt cgatttcttc ttttgcaact ttcattcgtt cttcgattac
     8821 ttcatctgca tctgttcctc ggcctacgat ccgtgatttt aattccgcta aatctggcgg
     8881 agtcaaaaag atgaatacac catcgggcac tttttctttg acttgttttg ctccttggac
     8941 ttcaatctcc aagaaaacat cttttccttc atccaatgtc ttgttgacat atgacaaagg
     9001 agttccataa taattcccga catattctgc gtattccagc atctctccag cttcgatcat
     9061 tgattcaaac tcttctttgc ttcggaagaa ataatcaacg ccttcgacct ccccttcacg
     9121 cattttacgg gtagtcattg agatcgaata ctgaaaatca ttatcctcac tctcaaaaat
     9181 tgcttttcgt actgtgcctt ttcctacacc ggatggacca gacaacacga ttaataatcc
     9241 acgctctgac atgacgacgt cctttcaact agtctttttg tacttatcgt ttattttgca
     9301 ctttttttct gcaactttca aggttatctt taattatagc tagaaattgg taaaaaagct
     9361 accctgtacc aagaggtcgt aaaaaagctg tcctgcatca agtaataaga acagccaaac
     9421 ataaacagct tctcatgttt ttcgtttggt tggggttatt atcgcagatg caagcttttg
     9481 aacgccgtgt ataagaggtc gtgaagaagc cgattagttt cgagtatgaa gaaaaatttt
     9541 gtgaaaatag tttctcacat ttttgcataa atttggctta ataccgagaa aatggttttt
     9601 gaacatcact tattcaaaat aaaaaaagat tgtgacacga attttgtcac aacctttaaa
     9661 tgtattccta gagcaaagaa gctaagcgaa gaaatccaat tctgattatt tattgtattt
     9721 ctttgttcag catccttgct aaaagcccat attggacaaa gtattcacta cacccatgat
     9781 ctcatctttt cgagaacgtg ctttttctgt cggatcttct acctcttctg tgtcaattcg
     9841 aacaataaat ttccgattca tcgtcacgta agcaccgtcg ttgatcaaaa taaacagttc
     9901 gttctcatta tagtctgcac aggcatcttt ccatacgtca aagccgtttt tttcagaagg
     9961 gatatcccgt ttaaccacag tttttccact taattcgtgg aacgttacgt cataatattt
    10021 cattatctct acctctttcg tcattcaata ttttgtactt gttctctgat tttttctaag
    10081 atcgtcttag actggacgac ttgattttta atttcgattg catttgattt ggaaccgatc
    10141 gtgttgattt cccgattcat ttcttggatc aggaaatcta attcccgacc tacaggacat
    10201 tgtgcttcga taagttcatc tagtttttgt acatggatcg ccagacgatc cagttcctcg
    10261 tgaatatctc cgcgttccaa caagatggct agctccgtca acaaccgttg ctcatcgact
    10321 ttcgctccaa gaaattcctc cagctttttt tgatacctta tttgaaattc cttttcatag
    10381 atttctacaa aactatcgag tttttttagt acttctttga attcttcagt attctttttc
    10441 aacactaaag ctaaagcttg accttctttt tctcgactcg cctcaataga atcaagtgcg
    10501 cttttcaatg tatctagagc taactgtgcc aattcttcca tgctctcttc cggtttttct
    10561 tctaccgtta cgaagttttc gtccagcgcg aagcgttccg ccatccgact aattgacact
    10621 tgattttgac taccgtatcg ttgttgaatt ccgtctgtca attgagtgat aagtgaatca
    10681 atcaattccc agttgatgac gatctgtttt tcgtttttgc caatataagc tagattcaca
    10741 aatacttcga ttcgaccgcg gcttaactgc tgtttaataa gctgacgaaa atcgttttca
    10801 acaaagttca ggattttagg acttcgtatt tgaacatcta gaaaacgctg gttgacactt
    10861 tttatttcaa tatctaactg atacgctttt gtttcgcgga tggctttgcc aaaaccagtc
    10921 atacttttca ttcgacaggg tccttcctca ttacatattt tctattgctt tttgcagttg
    10981 ttcaaactct tcgttcgtca atgtcaggcc tttccccatt ttttcatggt ctggcgccca
    11041 atctcgaaga tcgaattttg gcgggcgacc attccagctg actaggttca gttctttacg
    11101 ccaccccttg ttgttttctg acaatatagc gatttcttct acgatttcat atgaaaattc
    11161 ttgtgccatg gttgttcctc cttgagataa tataaatata ggatcagtaa tgtgtcaact
    11221 ggttgcttaa gcaattcatg ctgcaaacga tccttgcgtt ttccttgtag cagataatca
    11281 aaataaacag ttttcgtgcg ctcattttgt attttttgct ggtattttcg ataccttcgc
    11341 cattcttcag tacttaattg accaatttgt gccaataaat catccggcat actttttata
    11401 tcttgttggc agatcatttg aatcatcatg tctacttgat agatttcatt gggcagtaaa
    11461 aaatgttttt ttgctaaaag ttctagcatc gccaatccgc atgtctctgg tacgaccctg
    11521 ttcttatata aattacgcag tttttgccaa aattttatgc ttttgaaaaa agataattct
    11581 aactgaattt cttttgttaa gtaagacaaa aatcctcgcc agtatgttct tgcaggttta
    11641 acagaatgaa atgtatacca gttcccatag taatagccta atcgtttttt catttccaga
    11701 tagcctaatt gtaaatttaa ttggttcctt tggctatcaa aaagcagaaa gctgcctagt
    11761 gtccacaatg tttgacattt ccaatggata aaaatgtctg gcatacgaat ttttttcgct
    11821 gggcctggcc cttgcacatc tacgacaaat gcctctgttg ctcctttgtt aatcgcaata
    11881 tcaataggta ttttgttgcg atacccaccg tctgcatatt tattttttcc gatctttcga
    11941 taagccatag cgggataaaa tgaagcagag gcgagtatcc aatctggtat ttcttctgtt
    12001 tttgtctgtt ggatatcagt cactacttcc gctagttctg gcagtttagt agacaccgta
    12061 tataaacgta ttttgggatt atgtttgagt aaggacaaat ccaaattgtc tttgaccatc
    12121 ttttccaatg gagcagaaga aaccccgcct tcaattagtg ctgttttcgt catttggcgt
    12181 ctttctcgat agaagcgttc acgaaggtct tcaactgcag ccatttctgg taacgcaagc
    12241 acttgtcttg tcgacagctt tttccataaa ttcacagctt ttttcatatc tccttgtaaa
    12301 atcaatgcgc cgttcaacgc acctaccgag gtacctgtga taatctcaaa gttgatatca
    12361 tgttccttca gcgcctgcca cacaccaatc tgataagcac cgtgtgctcc tcctccacct
    12421 aaaacaagtg ctgtatgata gaccagcttt ttatttaatt gtgtggttcc ttgatagccg
    12481 tttttatgaa aaatacgcaa ccattcttct gatagaggag attgaaataa aaaacaacag
    12541 ttttctttaa taaaaaaacg agcgcaagct tccatatacc gaaaaaattt tttccattca
    12601 aaagtttcac cttcaatcaa cagattatta aaaataacat ctcctctact tttagcaata
    12661 gtaaaataga cggtatattt tttcgatact gctttaaaag cacattgctt ttttgcagaa
    12721 cgccaaaaag cgtctctact ttcttgatga ctttgaaaaa atggaagggg ttgttttgct
    12781 tgctggatag gcgaatacag cgacattttc tgtgaaaagt aaactttttt tatctccatc
    12841 gcgctctcct ttttcttcaa gtataccagt acgtcataaa aaggtcatga gtctcgtatt
    12901 cgactttgta gattccataa gaaaacaatt cataaaacat cctttaaata aaaaatacaa
    12961 aaatcacaaa aattaattga aaatgagagt gttttcaatg aaaacgtgat tttgtaatgg
    13021 aattttaaaa taaaagaaac aagctttttt aatgcaaatg ttgtaaaata aaagagatta
    13081 agaggaaata agaggaggaa catgatgact gagtcagttt atgtacatat tgataccaca
    13141 agtaatgctg ttttaacaaa agggctgact gctactgact ttgcaaacag tatcgtgcat
    13201 tttcctcaaa acttgctctt gcttgatcca tctgcttctg ctggagaata tgaatcccat
    13261 acagggctga aagtaatccg tggtacagaa aatattcagc gtttcttttc ctcttctcgt
    13321 aacaggcgtg gattcgatga attgaagtgg atcgacttta ctgatttaac gatgctgaaa
    13381 gaattgacac ctttagaaat ttcagaatta ttatattttg gtcatatgaa aacacatctt
    13441 cattcgccgt ttttttataa actacagaat aattttgtat acttcgatct taatgatcag
    13501 ctgaatcgga tttattatcg ctatttagat gaattttata ggatttttgc tagtaaatta
    13561 agttcgatct tgtcagataa gatcaatgta aaaaaatctt ttttcaaaaa agaaacacct
    13621 gttgaaaaaa tcagtgtgga attattgaaa gaaatgcgta atttgatgca ggaagggatc
    13681 attttttctt ttgatcaagt tggtttgatc gatggtaaat atcgtatccc aatctatgtg
    13741 attgaagatt atatttggaa attaaaaggc acccggtatc gagaagagga aatcatcgct
    13801 actttaatct actctgtcac cgatcgacac tggcaattgg tagaagatgc cgatctttct
    13861 tatctgccat tgaagtagat ctatttctta tcaaaaaata aaaaggaaac aagaaactag
    13921 gacatgattc tgtctcaagt ttcttgcttc ttttttattt acagctcctg tcgtttatct
    13981 ggcacaaaaa tcgaaggagt cgtttgttgg atccctaata aaacgatgat cgttacaata
    14041 gctgtactca acccgctggc accattcatt acgaaagaaa agagccaaga attcatcccc
    14101 catagtgcat acgctcccca gaaaaccact cctgcaataa aatgccaaaa atatctggaa
    14161 agtgcgccaa tcaatgcacc aaacacgatg ctttttctaa tttgtttcgt attatcgtct
    14221 acatagcctt tttggacatt tgaagccatg agccctgctg cgcctgcaaa agtataggca
    14281 atcgggtact caatcaacac ttgaacgaca cttaaataat aaacttgtcc caacggaaaa
    14341 tgtaggatgc cccaaatgaa acctgccagc atccctgcct ttgtccctct tcttaacgca
    14401 aaaagagtca atggaatctg ccctaatgaa atagaaaaac tgctgccgat ctggatcgga
    14461 atcaacgaca acaccatcgc taacgctgca acaatcgttc cttctaccca aactctattt
    14521 tttcccataa aaaaacttcc tcctttgaat aaaaaagtca caaaggagga agcagctttc
    14581 gaaaatagtc aaacagctgt ttattcacaa ttcctacgct cgcataaccg atacaggtac
    14641 gaagggttta gaaatagatt caatctcagc ccaattggac tcccctttgt gagtttattt
    14701 attatttatt ttttgtttca aacaatggac taactggttt gttttcatgg atacgtttga
    14761 ttgcatctcc cattagtccg ccaacactta cttcatcgat tttgtcgatt ttacgatctt
    14821 ctggaagata gatcgagtct gtaacaacta gacgctcgat agctgaatct tctattcttt
    14881 gtaaagcagg gccggataat actggatgtg tacatgaagc atacacacta gttgcaccag
    14941 cttctttcaa agcattggca gccaatgtga tcgtacctgc tgtatcgatc atatcatcga
    15001 tcagtacaca agttttgcct ttgacttgtc caatgatatt cattacttct gcaacatttg
    15061 cttttggacg gcgtttgtct atgatcgcaa tcggagattt caaaaattcc gctaatttac
    15121 gtgcccgtgt gacaccacca tggtctggtg aaacaacgac aacatcatcg cctttgattc
    15181 ctttttctaa gaaataattg gcaataagtg gcgcacccat caaatgatcg actggaatat
    15241 caaagaaacc ttggatttgg actgcatgaa ggtccaaagt cagcattctt gttgcaccag
    15301 ctttttgaat catgtttgct actaatttcg ctgtgattgg ttcccgtgca cgtgctttac
    15361 ggtcttgacg cgcatagcca taataaggca tgactacgtt gattgttttt gcacttgctc
    15421 gtttcagtgc gtcaatcatg atcagcaact ccatcaagtt atcattgact gggctgctcg
    15481 ttgattgaat aacatagaca tgcgatccac gaatactttc ttcaatgttt acttgaattt
    15541 ctccatcact aaattgagta acagatgatt ttcctaactc gacgccgact gctgctgcaa
    15601 ttttctctgc taatgggcga ttggaattta acgcaaaaat ttttaatctt gggtcaaagt
    15661 aatgttttga catggggacc tccactttct ttttcacaac tctagaattc tacctaataa
    15721 atagtagtca tattttttaa ataaatcaag tgattttgca aaagtcttag tttagataag
    15781 ggagtttttt tgcatatcct tctttattga cttgtcttgc tctcgcaatt gccagcgcat
    15841 attctggaat attgtctgta attgttgacc cggcagcaat cgaactgttt ttagctacct
    15901 cgacaggtcc gatgatattc gtgctagaac caataaagct gtggtcgccg acagtcgtat
    15961 gatgtttgtt ctttccatca tagttgacga agacaacacc acatccgaca ttgatttctt
    16021 ctcccagcgt agcatcacca acataagtca agtgaccgac tttagttcct ttgccgattt
    16081 gagcattttt cacttccaca aagtttccga tatggacgtt ttcgccaatt tcagcttttg
    16141 gtcgtaaatg tgcataaggt cccacatctg catgtttctt gacatggctg ctttcaatga
    16201 ctgagttttc aatgacgacg tgatcctcaa tcctactgtc tacgattttt gaatgtgacc
    16261 cgatgacaca atcagaacca atgactgtat ttccttggat ttgtacacct gcttcgatca
    16321 atgtgtctgg accgatttct acgcctgcat cgatgtatgt cgttgctgaa tcgacaaagc
    16381 ttacgccatt gaccatatgc atctggttaa ttcgctttct catgatctca tttgctttag
    16441 ctaacgcgat ccgatcattg actcccattg attcttcaaa atcatccgtt tgataagcag
    16501 caactgtatg gccttcttct tttaagattt cgatgatatc tgttagatag tattcacctt
    16561 gagcattgtt tgttccaact ttgcttaacg catcgaacaa agcttcgttg tcaaaacagt
    16621 aagttcctgt attgatttct tgtacaagag cttcttctgg tgttgcatct ttttgttcca
    16681 cgattttttc tacgatccca atatggtcac ggatgattcg tccatatcct gttgggtttt
    16741 ctgcttgtgc cgtcaagatc gttgcacttg cattttttcc ttggtgatat tcaaaaagat
    16801 tgttcagcgt ttcagtagtc aataatggtg tatcgccact gataactaac gttgttcctt
    16861 tttttccttc caagaaagaa gcagcttgca aaacagcatg tcctgttccc aattgttctg
    16921 cttgtaacgc atatttactg cgttctccta gttgtgcttt aacttgttct gctccgtgtc
    16981 caacgatcgt aatcacttca tctggtttcg tttcactgac ccgattaatg atatgttcta
    17041 ccataggttt accagaaacg ggatgtaaaa ctttatataa ttttgacttc atgcgggtac
    17101 ctttccctgc cgccaaaata attgcataac gtgcgtccaa ctgcgtcact cctaaatata
    17161 gtttgctgat ttcagtttat cccagtatac ccattttttc aacaaaacaa gtgattttct
    17221 tttatcccag cgttttctgg ataaaaaaat agatagacca taacgggcct atctatttgg
    17281 taaccggtgc tctcctgcca actcctcggt cacaagtcac actactcata aaacatgaaa
    17341 agctgttttt tgaaatcgtt tcttgtgact tagagttaaa cggtaggctt acaacctctt
    17401 cattaaacgt ttttcaatca gctgaccttc ggtattagct caataaacgg caaaatatga
    17461 ggagcaattt ttccgtttat tgagctaata ctcaggacag gacgctgatt tcacaacctc
    17521 tagataaacg gtgttcaaaa gccagtttct cggtattaag ccaaatttat gaaaaaatgt
    17581 ggaaagccat tttcacaaaa tttttcttac tactcgaaac taagcggctt tttcacaacc
    17641 tcttattctg aaaaataatt tcctggcaca accgtaatat ttttcgtttg agtatctaca
    17701 tctttgacat aaagcagtga cgtataatca tcgatggcgc gatgaccgtt gaattttgat
    17761 tcggcaaata cagtgatgcc aacaagttct gattcgaatt cttcaatcaa gctcttcatt
    17821 ccgttgactg ttccgccgcc tttcatgaaa tcatccacta caaggacacg tgaaccacgt
    17881 ttcaaacttc tcttagacag ttccatcttt tcaattcttt cagaagatcc agacacatag
    17941 ttgacactta ctgttgatcc ttctgttatt tttgagtccc ggcgtacaat aacgaatggc
    18001 gcattcagat aataggacac agcttgggca ataggtacgc cttttgtggc tacagtcatt
    18061 acagcatcga ttttttcacc aaggtattta gaagcaatga ttcttccaac ttgacgcagc
    18121 agttcaggtt ctcctaacaa atcagagaga taaacatatc ctcctggaag cagacggtct
    18181 tgttctgata gacgttctgc tagagattca atatattttt tcgcctcttc atatgaaatt
    18241 tcagggatga acaatacacc acctgcagca cccgggattg tttccaaggt tccataccct
    18301 cgttctttaa atgttttctt gatgattccc aaatcttcac tgatagaaga tttagcagat
    18361 tcataacgtt cggcaaagct tgttaaggaa atcaattcat gcgggtgatt caataaatac
    18421 tgtgtcatgt caactagacg ttcactgcga cgtactttca cctattccaa ctccttttaa
    18481 gaaattttat cgttttacat tatataagtt tttcttcaaa aattcgagca ttttatgaaa
    18541 aaataagaaa atagttcggt atttattaaa ataaaaagaa gcctataaaa aatttttcgt
    18601 ttttataagg cttcttttct ttataattta gttatttttt cttctgaagg caaccaatat
    18661 tcgtttgatg atatttacaa tcaggaacaa agcaataaag actaatgtga ttgttgctcc
    18721 tggaggtgta tctagataaa acgagctagt tagtcctgac agcatcccta gaaatcctac
    18781 acaaacactg atagcaatca cgacattgaa gcttttgcct aatcgcatac cgattgctgc
    18841 gggaagaacc atgattgcgg atatcaaaag tgcaccggca atcgggatca tcacagcaat
    18901 agccacacca gtaatcacat tgaacatcat cgacatcaaa tgaacgggta gcccatccac
    18961 gtgggcggta tcttcatcaa aagtcagaac atacatcggc cgtttgaata aaaagaacaa
    19021 tataccgatg gcaacgaata agattcctaa aaagatgact tgctcccatg taatggtcac
    19081 gatcgaacca aacagatagg attggatact tgttgctgaa ttccctccgc tcaaattcat
    19141 caatactaac gctagcgcca aaccaccagc cattaaaatg gcaatggaga tttctgaata
    19201 ggttcggtag atcgttcgca gatactccaa tatcacagct gctaatacaa cgactagcat
    19261 ggtcgtaagt gtaggattaa ggttcaaaaa gaatcctaac gctacccctg ctaaagagac
    19321 atgagaaagc gtatcagcca tcagcgattg tcttctgatc actaaaaaca cacctagcat
    19381 cggggcgatt ccggcaatga agatagcggc aagaaacgct cttctcatga attcatatga
    19441 aagcaacgcc attgagaatc ctcctttctc accagtcgga tctgtctgtc cgcatactgt
    19501 ttgatatctt catgatcatg ggtgatcatt agaactgatt tcccatggac atgcgcacta
    19561 tgtcggagaa gacgataaaa ttcatttctg gattcttcat ccatacctgt tgttggttca
    19621 tcaagaacaa ataagtcagg gtcagtagca aaaatacgcg ctaaactgat tcgctgcttt
    19681 tgtccgccag acaattcacc tattcgccga tttcgcatat cccacatccc tacagcttct
    19741 aaagcacgct gtacatgttg atgatctttt tcagagagac gcttgaacca gcggcctctt
    19801 ggatatcttc ctgaacggac taattcgatc accgtactag gaaaacctgc attgaaagat
    19861 gcaacttgtt gaggaatata accgatactt aatttttttc cttcttgatt tttctcagca
    19921 atctttatca ttccttttga aggtttcagt aatcctaacg atgctttcac caaggttgac
    19981 ttggctgctc cattttctcc agttaaaatg acaaattcac ccgcatctac atgataagag
    20041 acatcttcta gtacaggttc atcatcatag taaaaggtca aatcagatat ttcgatataa
    20101 cgcaccttac aacctccatt ctaaaactta attgatactt tcttttaaag ctttcagatt
    20161 tttttccatg acagaaatat aattttcgcc tgctttgatt tgttcattag tcaagctttc
    20221 taaaggattc aacacttcta gcttcactcc tgtttcattc gacaatgtct cagctacctt
    20281 cgaagaggca ttttcttcaa aataaatcac tttgatgttg tttttcttca caaattcttt
    20341 tagctcagct aagcgacttg gtgtgggttc ctgatctggt gaaacgcccg aaatcgctac
    20401 ttgttttaag tcgtattgat tcgctaaata agcaaaagca gcatgctgag tgacaaatgt
    20461 gcggtttgtt gcatctttta gctcatcgga gtatttttga tccagtgctt caagcttttt
    20521 gatataagca tcggcattct tcgtaaaagt ttccttttta tctggatatt tttcgcctaa
    20581 ttcttcagca atcgttctga cttcttttat agctaatacc ggatcgagcc aaacatgtgg
    20641 atcgtattca tgtgaatgac catgcccttc ttctgattct tcatgttcgt ctgcttcttg
    20701 cgattctaaa cggtcgattc ctttagctgc ttcgatcatc aaggtctgtt tgctatccac
    20761 tgaatccttt aacgaatcca caaaaaattc catgtctgaa ctattataca cgaaaacatc
    20821 tgcatcggaa atttctgctc tttcttttgc acttggttcg aaatcatgcg gctccgttcc
    20881 agcagggatc aaaagtttca cgtttccttc atcccctacg atctcttttg taaaatcata
    20941 catcggataa aaagtcgtaa cgatcgtcaa atcctctttt ttgtctgctt ccttgttcgt
    21001 atttccacat gctgcaaaca gtaaagcacc tatcattacc gtagctgtca aaagatattt
    21061 tttcatttat atgctccttt caatgaacaa atcggaattt tacgatttat taacgtgaac
    21121 taatgtatca aaataaaaaa acacagtcaa tagaaatcca tcaatttctg atttaagcat
    21181 gtatattctt gattaaatcg taaaatggat gagttgctgc tcatatctag ccatttcact
    21241 acaataaaaa actgaagaat accattttag gattcctcag tttttcatca cctattttac
    21301 ttcttatatt ttattgcagg gttcgtacta aataaacttc ctcacaaaaa ccttttaacc
    21361 cattatatac tctctgcgct cttgaataat gttgacacaa ggcaaaaacg gttggtccgc
    21421 ttccactcat caatgcagca tctgctccgt acttgatcat acgctctttg atttgatgga
    21481 tgactggatg ccgctttccc gttatatctt ctaaactgtt tcccattttt ttgatcatct
    21541 gctgataatc ttgctctcgg atggcttcag caagtccgtg gatgtcttga tgttggatgg
    21601 cttgcgtatc tacttctcta aacactgtac gtgtagagac actcattctt ggtttgacca
    21661 acacaaccca gcactgtggc atagaaggta atacttggat ttcttcccct ttgccaccga
    21721 caaatgccgt acttccgtag acacagtaag gaacatctgt accgacttcc aaaccgattt
    21781 ctgccagttc ttctaacgaa cagcccagat tccataattg gttgatccct cttagagcag
    21841 ctgcacagtc tgtacttcct ccgcctaatc cagctgctac tggaatattc ttcttaacaa
    21901 agatcttgac accggttttg atattgtatt tattttttag caataaagca gcttgataaa
    21961 cattgttccg ttcatctacc ggtaaaaatg ctttatttgt ttctacaatg attttgtctt
    22021 ctgttatttc ttcaaaaaat agatgatctg ccaagtctac gctcgacatc accatttcca
    22081 actcatgata cccatcttgc cgtctatata atacatccag tcctaaattt atcttggctg
    22141 gcgctttttc aatcaattcc atcctctcac ccattcgaaa agtctaagtc gcaagtattc
    22201 tatcataggg aaaaataaaa gtatagctta tgtctgccta gtggttgatt ttgtcatcgt
    22261 ttcaacagct ttcacagaat tttctcttca taatccaaac aagttctagt gattcttata
    22321 attctgtgag cacttcttct gccaaactcg gaacatttcc tttttctttt cctatcagac
    22381 tgagtagctg catggtagca aatccgaaat tacttccttt cctataagca aaataacgtc
    22441 cttcccaatg atcgacatat tttaatttct tttcataagc agactggaag gaatagaaaa
    22501 aattaccata attatatacg atatgcatga ctttttcttg aaagaaagaa aaatctgttt
    22561 ctccaacatt agctaatgga gctaagccta gattggcttc cgagtagccc attttctcgt
    22621 atacaataaa cagattggct aacagatagt cagctaattc ctcagattct ttaggtaaga
    22681 cccgcaacaa atcatatccc gcctgcttct tcgtcatcgg ctgagcggtg atgaagccaa
    22741 cgactttttg atctttacgg acaataccga caccgctaga taatacatat gatttatcaa
    22801 atcgtcccac agagaagtat ttttctcgtg caccttgaag ccatttttgt gaaacttcat
    22861 ttacagcttg atgtaattct tccggcagtt gatcgtacca agtaaaatgg tatccttctt
    22921 tcatcaaacg ttgatattca agttgctgac tcagccgaga caagtcttct gttgctagat
    22981 caacaatccc tgcttcacct actttcgtga agtgaaaacc caattcatga agcatcacta
    23041 cgtagctttc tgatatctta taaaacgcaa gctgatagcc taaacaatct gcttgtttca
    23101 taaactgatt cgttgctgcc cgcatcattt taggattgcc aactggatct cctaaaacaa
    23161 agcatttgtt tcctttgatc tgataaccaa aaacgacttg atcttggtcg tcttcttggt
    23221 aataaaaata actgtaacct gacaagcgaa gctgatggct tgtagctgtg cctccgtatg
    23281 tttccaataa acggtcaaaa cggtcttttt cgtactcact gccaagcttc gtagtatctt
    23341 cagctaaata ttgataaagc gccactaaag ctagcaatga gatgcccaaa ccaataagcc
    23401 ctgagaacca cacatcatct gaaggaaaca aaataaaatg atttcctgtt attcgaccga
    23461 ctaaaaagcg tccgctgtga tagcctgcaa ttgcgtaaac gataaacaaa aagccgaaca
    23521 agctagcatc aaagattata gctccccaag aataaacgaa ttttgttcga taaaattctt
    23581 ttctggataa ccacacagct aacagaatca agacatatac caatatcaac tgccaagata
    23641 acgtacgtgt gattgtattt aaaataccaa aagatagtaa agcaatcgta ggcagaaatg
    23701 cctttttgac ttttactgaa agtgctctag caaggccgag taataaaaag ccaaccaata
    23761 gattcactgt ctgatctaaa aaattgaatg agaacggcaa taatacttga aatagccgac
    23821 tgattgcaga cagattagtg attgttgata gaagtaccat catgatcccc gcaaaataga
    23881 cagcacttac gagcacgata tgcgcaacct tttggaaaaa cagtcgcgga atgttatcta
    23941 aaaatcgatt gattttgact cctgtatgtg tgatgaacaa tagaagtccc gtcataaaag
    24001 gtagaacata ataaaacaac cgataatata atagccaaac tactgctaca tcttgccgaa
    24061 cagacaattg actcattcct aagatcatca aaacgtcaaa agtccccatt ccacctggga
    24121 ccatcgtcag cattcctatc aatgtagcaa tcatgaacat aggatagatc gaaagcatcg
    24181 aaatatctat tctcatcaac ttgccgatga tcaaaaatac cgcaagggct cccgtccatt
    24241 gtccaaagga tgccagtagc agaccaagca agccttttgg ataaaactcg aagaacaatg
    24301 tctttctctt tagataagca aaaaagaaaa tgactggtgc taaaaaacta ccgccgaaaa
    24361 gccagatcca atagcttcga aaaatacttt ctgactgaat aaaaaagaca tcaataaatg
    24421 taagaaacga ccatacagac aaaccagtca gcataaatag tgcgactttt gatacagtgg
    24481 ctaaaaccat ttttctattt gtctgctttc cataaaaact agcccgcaga cttgctccta
    24541 ccacaccacc aaaaccggcg agattgttga tcgtgtttgt tacccacgca gcaagaagaa
    24601 actcttttct ttccattttt ggttttcctt gtttttctaa gactctgatc gtcacccaat
    24661 catacaacaa catcggagtg acacccagca gtccagccag aatcatgagc aggatcgtca
    24721 tgcggctttg ttgtcccatc ctttgccaaa tatcttgcca actcatgcca ttagcaatat
    24781 ttgcgacttg atttgccaca aagactaaca cggagccgaa aaaaatcagt tttaaaatca
    24841 gactatgttc ctttatccat tgcagcatca agtatcgttt atttttcaca ttttcccatt
    24901 cctaacttgt atacttttgt gtcatacccc aatgttatct tatttatttt aatttttcca
    24961 ctttagacct gttctttgct tgcgatgata gaaaaagaaa gatatcctaa tgctaaagga
    25021 gaggataaca tgaaagtagg atttatcggc acagggaata tggcacaagc tattattgat
    25081 ggtttattaa aaaaaggaac agtctcgaaa gaagaggtgt tcgtttccag tggacatttt
    25141 gaaaatgcac aaaaatttgc tgctgaaact ggcgtccatg catgcaaaag taatcaggaa
    25201 gttgcatcac aatcagatat cgttctttta gccgtgaaac caaaaatcat tgaaaaagta
    25261 ttggaagaac taagagaata tagccagaaa gtcttattca tttctattgc ttcaggaaaa
    25321 tcattaacag atttagaaac agcgcttgaa gatcctcagg cctccatcgt acgtgtgatg
    25381 ccgaatgtca atgtcagcgt aggagcaggc gtttctgcga tttgcaaaaa tgaagctgcc
    25441 acggaggaac agcttcagca agctacgatg atttttgaag caatcggcag cgtctatcct
    25501 ttagcagaat cagatttcag tacattcatt ggccttgctg gcagctcacc cgcattcgtt
    25561 tacatgctga tcgatgcgat gggacggact ggtgtactga atggtttgtc caaacagaca
    25621 gccactgaga ttgctgcaaa agcaattatg ggttcttgtc aaaaattcct ttcaagtgat
    25681 aaaaatccat gggaacttgt agaccaagtt tcttctcctg gcggtacaac tgttgcaggt
    25741 gtagttgcct tagaagaagc aggattcatc ccagcaatca tcaaaggaat caatacgacg
    25801 atcgagaaag atcttgagat gttgaataaa aaataatgat agcaatgttt tttcgtgcct
    25861 tacaaaaaaa gaagagctgt ggcttttgcc gcggctcttc ttttgtattt tttattattt
    25921 agcataatca gttgcacgag tttcccgaat aacgattact ttgatgtgtc caggataatc
    25981 cagttcttct tcgattcgtt tacgaatatc acgtactaaa cgaacagcgt ctaaatccga
    26041 gatttcttct ggcttgacca ttacccgaac ttcgcgtcca gcttgaactg cgtaacttga
    26101 ttctacaccg gcgaagctgt tcgaaatatt ttccagactt cttaaacgac ggatgtagtt
    26161 ttctaatgat tcacttcgcg caccaggtct tgctgctgag agcgcatctg ctgcagctac
    26221 aaggacagag ataacagaag tagcttccac atctccatga tgagaagcga tcgcgttgat
    26281 tactactggg ttttctttgt acttggcagc taattccgca ccgatctcaa cgtgagatcc
    26341 ttcaatctca tggtcaagtg ctttcccaat atcatggagc aacccggcac gttttgctaa
    26401 ttggatatct tcgccaagct ctgctgctaa gataccagct aattttgcaa cctcgaccga
    26461 atgcttcaag acgttttgtc cataactagt tctgaatcgc agacgtccga tgatcttgat
    26521 tagatctgga tgaagtgtat gagcaccgac ttcaaacgct gcctcttccc cgtattcgcg
    26581 aatacgttca tccatttctt tacgagattt ctcgaccatt tcttcgatac gcgctggatg
    26641 gattcgtcca tcttgtatca gtttttccaa ggtcatccgt gcgatttctc ttcggattgg
    26701 atcgaatccg gaaagaacaa ctgcttccgg tgtatcatcg atgattaaat cgataccagt
    26761 cagcgtctca agcgttcgaa tattacgacc ttctcgtcca atgatacgac ctttcatttc
    26821 atcattcgga agggtaacga ctgaaacagt cgtttctgaa acttgatctg ctgcgcagcg
    26881 ttgaatcgct aatgataata agtttttcgc tttgcgatct gcttcttctt tggcacgctg
    26941 ttcagattct tttaccatta gagttaactc atgattcagc tcttcttcag tcgatttcat
    27001 gatgacatct cgtgcgtcct cttttgataa accagcaatt ttttctagtt ctttttgttg
    27061 atcttcaatc attttttcaa cgtctttttc tcgctcttca attaattgct gttttgaact
    27121 aagtgcttct tctttacttt caagtgagtt ctcgcgtttt tctaacgagt catctttacg
    27181 attcagcgtt tgttcgcgtt gaatcaaacg attctcttga gattttaatt ctaacttact
    27241 ttctctcagc tcactttcaa cttcagaacg gtatttctga ttttcttcct tagcttctaa
    27301 caatgcttct ttttttagag tttctgcttc tttttttgca ctatcgatga ttccagcagc
    27361 tgatgattgt gcaccagcta tttctttctc gtgacgtgac tttgcgacca taaaccctaa
    27421 accaagaccg acaattaaac cgatgatagc gaggagaatg ttgaatacca taaaatccac
    27481 ctccatacta tctttttcat ttttatcatc tatacagctt ctatgataaa ttattctaaa
    27541 ctcgtcaata tgcatcttcg catatatgtg tacaaaaaac atgcacaact ttattctaat
    27601 gtttgtaccc cgtagtgtca actttaaata aacaaagaaa cggctttcta cttattcatt
    27661 aaacgatggt ttttaaaata aatgacacta gtttcatata ggtaaacctt taaattgaga
    27721 aacttttcta ttaaattatt ctttgtagat gaacagatat ttttatttta tgtacccact
    27781 cgtataatta aataactcac gatttcttta ttcaataaaa aaacggcagg tcaaggagag
    27841 tacctactct tcagaccagc cgtttttgtt tatcataatt tttattcttc tactaaaggc
    27901 aattcttctt gtcctttttc atcttctggg acagcgactt cttcaccaat accataagct
    27961 gctctaacta gagcagaaac ttctgccatc atttcaggat gattagccat atagatttta
    28021 gcgttttccc gtccttgacc aatccgatct tctttataag aataccaagc accgctttta
    28081 tcaacaatgt ctttttctac tgccatgtct agaagttctc cttcttgtga gatccctaaa
    28141 ccatacatga catctacttc agcgattttg aaaggaggtg ctactttgtt ttttacgact
    28201 ttgatctttg tacggttacc aacaatatct gtaccttgtt tcaattgttc tgcacgacgt
    28261 acttccaaac ggatcgttgc gtagaatttc aatgcgcgtc ctccaggagt gatttctgga
    28321 ttcccaaaca tcacgcctac tttttcacgg atttggttga tgaaaatagc gattgttttt
    28381 gttttgttga tcgaaccaga gagtttacgc aatgcttgag acatcaaacg tgcttgtaac
    28441 ccgacatgtg agtcacccat ttcgccgtcg atttctgctc gtggaactaa agcagcaact
    28501 gaatcgacta ctacgatatc tacagcccca cttgatacta aagcatcagc gatctctaga
    28561 ccttgttctc ctgtgtcagg ctgtgaaaga agtagttcat cgatattcac acctaatttt
    28621 tgtgcatatt gcggatctaa cgcatgctca gcatcaatga aagcggccgt tccgccattt
    28681 ttttgtactt ctgcaatagc gtgtagtgca actgttgttt tacctgaact ttcaggtcca
    28741 tatacttcga tgatacgtcc acgggggtaa ccgccgacac ctaaagcgac atctaacgca
    28801 agagagccac ttggaattgt agagatttgt tggtcgattt tttcgcccaa tttcataata
    28861 gagcctttac catagctctt ttcaattttt ttcagtgcag catctaaggc tgctttacga
    28921 tcatctgcca atacgataat cctccttata tttacaaatc agagcaacat ttcattatcc
    28981 ttatcatatc ttttttagtg aaaaaaagca agaaaaaacc gaacaaatat tcgcatttat
    29041 cattttctta aaagctgttt tctcaaaaga tcaaatcctt gcataaccgc attatatctg
    29101 atagcagcac gatctctggc aaagtgatat ttttccgaaa ccacgccgtc tttagatgaa
    29161 atagcgatcc atactgttcc tgcagattga ccttctaacg tgtccggtcc agccactccg
    29221 gtgaaagcta cactgtaatc tgttcttgct atctcacgtg cttgaatggc catctgctcc
    29281 acacattctt tgctgactgt tccatattct gctaatagct ccggatcaat ctccagaaaa
    29341 ccagcttttg tttggagcga gtaggtaacg aatccgcctg gaaatacttc agatacacca
    29401 gcaatgtcgc ctaatgcggc ttggaaggcg ccggctgtca aactttccgc tgctgttaca
    29461 gtctgcttgt tttctttcag tagttccact acgacttttg ctagcgaatt atcatcgccg
    29521 tatccataaa aatattcccc tactcgttct tggatttttt cttctaatgc taaaagagct
    29581 tgattccctg cctgaacatc atttgttttc actgtcagtc gtaatgtcac ttcatttggt
    29641 ttggcatatg gtgcgatcgt tggattgatc tgtgtctcaa tcagatcttt tagctcagtg
    29701 accagtcgcg attcaccaat cccataaaag cgcaaaactt tagaaattag tttttcttca
    29761 gaaggaaaac gttcctctag taaaggacgt acttgttcca caaacattgg tttcaactca
    29821 ttaggcggac cgggaagaag gatatacgca tgtgtatctg tttgataaaa agtccctaaa
    29881 gcaagccctg tacgatttgg tagaggaacc ccgccttcga taatctggga ctgttgcaga
    29941 ttgtttttgg tcatttttcg atgggttgtt tcaaaatatg ccaataattt tttgtatcct
    30001 tccgtatttt gtatgagaga cttaccgagg tgagccgcag ttgcttcttt tgtcaggtca
    30061 tcttctgtcg gtcctaatcc gccacataag agtatgagtt cacttcttgt ttcagctaga
    30121 gcaatcaact cttctaaacg ctgctgatta tctcctacaa ctgtttgata ataaacttcg
    30181 atacctagat cagctagctg ttctgacaag aaagtggcat tcgtattcac gacttgtcct
    30241 agtaaaagtt ctgttccgac agctattatt tctgctttca ttacttcccg ctcccttata
    30301 tagtatacgc tgtattttta tattttttta tttaaaaaga gggcaaggag ctgtgacaaa
    30361 aatcttgtca cagctccttg ttccaaataa acagtgttct cctgccaaaa cctcggtcat
    30421 aagtcacaat atacataaaa catgaaaagc tgttttttga aattatccct tgtgactcag
    30481 agttagacgg caggctcaca acctctccac taaacgatac ttacattgat cctttgaaca
    30541 caccgctgtt tttgatgaag taatccacac ctgaatagat agtaaagatc agacaagcat
    30601 aaagcatgat ttgatctact ggaataccga tcaggctgaa tgggatatta ttgataaaca
    30661 gcaagataat agcaaccatt tgtgtagccg tcttgacttt tcccggccaa gctgcagcca
    30721 tgacttctcc atgttcgaca agtagtaaac gcaagcctgt gacggctaat tcacgacaga
    30781 caatgatcgc tactacccaa ccaggggcct ttccttggtc aaccaacatg ataaaagcag
    30841 tcataactag cattttatct gccaacgggt ccgcaaactt gccaaaatta gtgacaaggc
    30901 ctcgcgcacg ggcaattttt ccatctaacc agtctgtgat actagctaaa gcaaaaataa
    30961 ctgcacctac tagctgtgtg acagccagtg tggtgtctcc tacgttaaat tgtccccaat
    31021 ctaaaggtaa acctacaacc aaaatgaaaa tcgggatcat acaaattcta agtacagtta
    31081 atttatttgg taaattcaca ctatcgactc ctttcttccc tattctacgt gactatcagc
    31141 gaagtttcca ccgatattat ttacttttcc aaatgaattt attcaaagga gtatttttat
    31201 tgaaattgat ctttttgaat taacgaagaa ggaccgcgac acagatttgt cacgatcctt
    31261 cttttttata cctacaagta aacagatgcg taaactaatt tatacgatca ttctgcgtat
    31321 tgaagagtaa ggttcacatt ttttggactc tgattgtttt cccctggatt aactggtacg
    31381 gattctccat tcgctttgat cgttgcattg cttgctgcac caatcgtgat ggtcgcattt
    31441 gttgctgttt ccggtaacac ggtactctgt gtttcatttg ctgccagtgt cccttgataa
    31501 acataggcat tgtctactag cacacctaac catacacgat tgacagcagt aaattcaagg
    31561 gttacaggct tggtagcgtc tgtgatattt actgcgatgg cactgccaga atcttctcct
    31621 gtagctattg ccattttctt ttcttcactt gattttgttg ttgattctac tgtagaagac
    31681 tctttggtct gcgtgctttg ctctgaggaa gaggactcct cgactgatcc ttctacttta
    31741 agcgaagaag ctgtgtctcc gattattgga tcagcttgac gatcttgcca agtcatatag
    31801 cccacgacaa cgacaatcgc caaagcaacc aatcctaata aaatgacagg aagacttgtc
    31861 caaaagcgac ttggatgcga ttcttcctga tgcattgctt ttctagaagt acttactgtt
    31921 tcaggctgcg gacgttcagg gaccgatcct gtcaatttgc ttttcccatc aaaaacatcg
    31981 actaaatggt ctccatcttc tcctacagct tcagcgtatt gccgaataaa tgcccgcacg
    32041 taaaatttac caggcaattg gtcaaaatca tcattgtcga ttgcttccaa ataacgtttt
    32101 tgaatttttg taatctgctg taattcatct aaagatattt ttttattcag gcgtgcttgc
    32161 cgtaattttt ctccgatggt ttcaaaagcc actcgttcat cttctccagt ctgtttgatt
    32221 ctccgaagca tctgctgttg caacgaaaga cgttatgttt gggtgctgtg acaaaagtca
    32281 tgtcacagct ccttttttcc gaataaacat tgttctcctg ccaactcctc ggtcacaagt
    32341 cacaatatcc aataaatata agaagctgtt ttttgaactt gttccttgtg actcggagtt
    32401 aaacggcagg ctcacgacct ctttcttaaa cggtgttcaa tcagctgttc ctcggtatta
    32461 gctcaaaaac cggcaaaata taaggagata tttttccgta tgtttctcta atactcaggt
    32521 caaaacgctg atttcacaac ctcttcctta aacggtgttc aaaagcttgc atctgcggta
    32581 ataagtccaa ccaagttaaa aacattaaga agctgttttt acttggctgt tcttattact
    32641 tgatgcaggg cagctttttc acaacctctt ggtacacggt gttccagaag tagcttcttc
    32701 ggcaataagc cgaaatatca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt
    32761 tcttgaagct gaccacttct gtcacaacct ctttgattac ttcgtttgat tttattttaa
    32821 tataagttga ttaaaagaga gcatgatttg ttttattttc tcactaattt ttccaacaca
    32881 actaagaata ccataaaagc tgattttgga ggaggttcct actacaagtc taaaacgaaa
    32941 tttaagactt ttcctcataa aatactggtt tattttggtc tcatgtaaaa ctcgctgaag
    33001 gcctcttcac ttataaatgc ttccccagct gccagtacgt ctttcatttg gatcgaatcg
    33061 atgatttcag gaagatcaaa taaagttcga tcgccaaata aactttgcgt aaattgattg
    33121 gcaatatatt cgatcgaatt taatgattgg aaatattgtc caagcatttt cttcttcagc
    33181 aaatcgagat ttgtttctga cacttccgga tcatcagcga atccaagaat gatttctttg
    33241 actttttctg ctgctttctc tggttcgtcc gtatctccgc tgaagtccgc aaagtgaaat
    33301 tcacgatcca agctgaattc aaaaccaaag ctgtcatcga taattccttg attgtacatc
    33361 gctagatagt ttcgagaagt attgcctaaa atcatttgga acaacagatt gattgctgtt
    33421 ttatagcgta acagttctgt tccttcttgc ggcaatgtgt ctaatccttt aataccaagt
    33481 acaaatttat ctcgtgtaat ggctgcttcc aatgcacttt gtttgatgat ttcttttgtg
    33541 ttttcaggga aataacgtac aatttcttgc tttggaggga aattttttgc ttcttggttt
    33601 tcccgaataa gtttcatcaa tttctcaggc tccatcttac caactacaaa taaaaccata
    33661 ttgcttggct gataaaacgt ccgatagcac gtatacaagt cttgagctgt gattttgtcg
    33721 atactttcaa ccgtgcccgc aatatcgata tgcaatgggt gtgtaggata caagttattt
    33781 agtatgccaa aaaacatccg ccagttaggg tcgtcttcat acatttggat ttcctgaccg
    33841 atgattcctt tttccttgtt tacagtttct tctgtgaagt aaggtgcttg tacaaaatcg
    33901 atcagagtcg tcagattctt ttctacctga tccgtcgtag aaaacagata gctggttttc
    33961 gtaaaacttg tgaaagcatt ggcggaagct ccttgtttcc caaataattg aaagacatca
    34021 ccatcttctt tttcaaacaa cttatgttct aaaaaatggg caattccatc tggaactttc
    34081 accttttctt tttcaccata gggaataaat tcgttatcga tcgaaccata attcgtactg
    34141 aacaaaccat atgtcttgtg atagtcattt ttaggcaaca aatacacggt caaaccgttt
    34201 ggcagtactt catgataaag tgtttcattg atttgctcat attctgtttt attcatttgc
    34261 atttcctcct gctaaaaaga agattgcctg caattcgatt tgctcagcta cttgttgtat
    34321 ctctttgatc gttacgctct ccatacgttt caaccattct tcatcagata attttgtttc
    34381 cggcagccag ctgtccaaaa aagaagcttc gattgccgct tgcggagagt ctagagaaag
    34441 cagatattgg tttctcagca ttgcttttgt ctgagacagt tccaactctg ttatttcacc
    34501 atttcgcaaa ctttctaatt gctcgtgaat cagatgaagt acttgatttc ggttcttttc
    34561 atcgataccg gtttggacac tcatgaatcc tcggaacgta tccacgctac tagaggcata
    34621 ataagctaga ctttctttct ctctgacatt catgaaaagt ttagagtgag gaaaaccgcc
    34681 aaataaaccg ttaaagacca ttagcgcaaa acgttctggt tcatcgtaat acacatttgt
    34741 ctgataagct aggttcagct ttgcttgaac gattggctca cgtacttgtc gttccttcac
    34801 gatattcact tgaggttggg tataaaacag atctggataa actgtttccc gcgactcaaa
    34861 tggtaattgc ccaactgctt cagctgcttt gtccggatca acatcgccga caacaaaaat
    34921 gtcgatttga tcatttgcca tcatttcttg atagtaggac gctagcgaac tagcagtgat
    34981 cttgtccaaa tcttcgacta cgcccaaccc tgggatcttt tgttccgggc tgttttcaaa
    35041 ataaagttct tgcaaggcat aggaagcaaa agtttgttta tcttccttca aactttccaa
    35101 atatagacgc agattgttct tttccaagtc aaacgtcaac tgatcaaact gatgattttt
    35161 aatatttgga taaaataaaa cctcttttaa aaaatctacc gcttgagaaa aaaggtttgg
    35221 atcattgaca tattcaccat tcacaaaact gatccctgcg tttagccaat gtaaatttcc
    35281 tttttttcta actgataatc caaagcttgc gccataaagc tctgcaagtt gcgcacttaa
    35341 ctttgtctga tccggataat tcaaactatt tgtttccaac atgctgctga gaagtgaacg
    35401 tttcgtgatg acttcttgtt gcagacgcgc agtaaaacgg ataaaaatcc ggatcgtttt
    35461 gtattgtttt gttggtaaga catgaagatt cacgccttct gccaattgat acaccatatt
    35521 tatatgctcc ttttcacagt tgattgcccg cttactcttt taatgggtca acctgccttt
    35581 tttacttatt cctctgcttg aaatttataa gttcaggcaa ttcgccgttt tttctaaatg
    35641 atgttacact ttatagtata gcatagatgt tttttatcaa aagaaacaga cggctaaaag
    35701 cgtatattgt ttttttattt tttatttttc gatatactaa agacaaataa aagaagggag
    35761 ggtcattcat gatcaaagaa ttcaaagagt tcatcatgcg tggagatgtc ttggatcttg
    35821 ctgtcggcgt tgttattggt agtgcgttta caagcatcgt aaaccaagtg gtgcaaggct
    35881 tgatcacacc actaataggt tggtttgtcg caatgatcac aggtacttct gatttagaag
    35941 gagcactttc aattcttgac tggtcgccga caaaaggcgt cacatttgcg tttggtaacg
    36001 ttattagtgc tatcattaca tttctgatca ctggttttgt cttattctta gttgtcaaag
    36061 cggcaaaccg tgcgaagaat ttacgctcca aagaagagca agtggaagtg gaagaaactc
    36121 ctactgcaga agattatctt agtgacatac gcgatcttct tgccaaacaa gtggcagaag
    36181 acaatacaca tccagtagag caaacaaatc tttccgatac ttctaaataa taaagaatct
    36241 aaaaaaaaga aagccgattt tcagtgtcgg ctttcttttt ttagattctt aacttgctac
    36301 ttgttccttt ttcatgtcga tatgtggaat atcatcttcc aaatacacat ctgagattgc
    36361 cttgaatcca aacgactcat aaaaagcttg taggtattct tgtgcgccga tcacgattgg
    36421 tttttctgga aaatttttct ggatcatgtt aatcgtttct tgaacaatct ttcttcctaa
    36481 accatcttta cggttgtcct gatgaacaag gacacgtcca aaatggatac tctccgcttc
    36541 ttcatatact cgcgtgtagg caaggatctt cccatcagta tcttgaaaaa acgtatgcca
    36601 agcttgtcca tcaatgtcat caacttcttg ataaggacac tgttgttcca caacaaaaac
    36661 tgcgattcgt tctttgacga tcgcgtaaaa ctctgctgtt gacaattctt caaatttctt
    36721 tacctgatat tccactaagc aaacgccccc tgctttttta ttcttagcat accgattcgc
    36781 acacaaaaaa gcaaacgaac cttacgaatc tcaggctcgt ttgcttttta aactgcttca
    36841 gttaaaaatt cgatctcaac tgtccgagta agtacatctg aatagctgta agatactcgt
    36901 tcaaaagaat tttcttcagg atccaaatca acaacgaata ctgaaggata ggtttctgtt
    36961 aaaacaccgc ggcgttctgt ttgacgcttt cttcctgtct gagcaaccaa catgattggg
    37021 ctgccgatac gaccttctaa atctttcttg attgaagcta aagttgttgg catacagttc
    37081 acctctacta tcaactatac cacatatttt taaaattttc aagtttacca ttataaaaaa
    37141 agaaatgcaa taaaaataaa taagaagctc attaagattt ttctaaatta ttcctcatta
    37201 taaaatttca agtgtttttt tattttgagt tttactctcc cgtaagcgct cattacttgc
    37261 ttgtgatcca ttgctaaaca ttcagccatg tctacaaaat caacgccttg catataataa
    37321 tgaaaaattt tagcttctat ttttgaaaac tggatttcgc aattttcgat tgcttctttt
    37381 aataaaagat gctgaatcgc taaaggttct tgcaatgtga tctctgctaa aaagtctggt
    37441 ccttctgtct ctattttttg gtcgattgag acttcatctt tttgatcacg acgcttcagc
    37501 gcgttctgct tcctcaacag agaatagatt tgattgctta taatgctttt ataataactt
    37561 ccaaagctaa cacctttgct tttttcgtaa gttctcaatg cctgatataa agcgatcctt
    37621 gcttcttgtt cccaatcctc agaaacataa tttcttaaat aataggtgtt tttcaaacgt
    37681 agtaaaagtg gaaaatattt ttgatacagg gcagcaaatg cttcttcgtc accattacgt
    37741 gcagcgcgca ataactcctc agacataaaa taattcctcc ataaattcgt ttatggagga
    37801 atcttatcat caatgattca tcgaactgat tagaacgata tgttatttat tttttctcat
    37861 taaatcgtcc aatttttggg acaatttttt taactgctct tcattccatg gagaattacg
    37921 tcggaaatca ataaaatgca tatctttatt ttcttgggct attgtttttt ccgttttttt
    37981 tatcgtctta tataattcat aagcagaagt tcgtaatgct ccttgggaaa agatcatcca
    38041 ctgttctgct aaatcgcttg tcgccactgt cacttgtgtc aaacggtcat tcagctgacc
    38101 agcgattctc tcgatataac tgtcagccgt ttcgtcttct ttcgtaaaaa caacatccaa
    38161 ctggtatttc ttataatttt gttggatgcc cggaactagt tgggcatcga agacaacgat
    38221 gacttccatt ccttcatatt tcgcataatt tgataaacga tgtaaaagag cttctcttgc
    38281 atccgccatt ttgtcctgct ttttaagctg gacaagttcc ggccacgaac caatcatgtt
    38341 gtagccgtct acaatcaaca gctgtttttt catggtctac tccccctgac gttgggaaaa
    38401 agcctgatac ataagcaaac cagcagcaac actggcattt aagctctgga cgtgcccggt
    38461 catcgggatc gtcagcaatt catccatttc ttttttcaat cctggactca ttcctttgcc
    38521 ttcattacca atgactaaag caattgatcc tttggcattc catttttgga agtttgtgcc
    38581 ggtcatatct gtgccaaata cccaaaatcc tgtttctttg agctgttgca cagcttgtac
    38641 caaattcgtc acgcgtgcaa cagggacatg ttcgactgcc ccagtggaag tctttgttac
    38701 gataggtgtg attccaactg ctcggtgttt tggtatgatc acaccatcga cgccacttgc
    38761 gtctgctgtc cgtaaaatcg acccaaaatt gtgtggatct tcgatgctat ccaaaatcaa
    38821 aaagaagggg tcttcttttt tctcttttgt tcgttcaatc agttggtcaa gggataaata
    38881 ttcataaggt gtaatcgcaa gaacgactcc ttgatgcacg ccgtgatcgc tcaaagtttc
    38941 taatttttgt ttaggaaccc atttgactgg cacagcttgt tcacgtgcga tttctttcag
    39001 ttcttctact ttttctcctc gtgaatcttc ttgaagaaag agtttattcc ctcgcccttg
    39061 ttgtagcgct tctactgcgg catggtgccc aaagacgaaa ttatcttcta atgcttcttc
    39121 attttgatca gaaactgact gatttcgtcc agtttgcttt ttatttgaat atctattttg
    39181 ttttttgtct tcaaatccac gtttcgattg atgtttgttt gttctttctt tacccatttt
    39241 tttcaccaac tttctgaata caccagcgga tcaattcttc catccgctcg gtttgttttg
    39301 tcaaatgcag atagcccatg agcgcttcga accctgttgc gacccgatac gtcgtaacat
    39361 cagcattttt tgcgctcgta tggcttttag catttcttcc tctgcgataa taaagttctt
    39421 cttcttctgt caaaagatgt tcttccagca tttgctgaat caaaaaggct tgtgcattcg
    39481 cagaaacata attcgttgcc gcacgatgca gcttattggg ttttgtttct ccttgatcaa
    39541 tcaggtggtc acgaacatag atttcgtaaa tcgcatctcc aacataagcc aaggctaaac
    39601 cgttcaattg tgtgtaatct ctcatgcttc tcttctccat cgggttcctt gcggtgtatc
    39661 ttctaataag atcccttttt ctttcaacaa atcacgaatt tcgtcgcttc ggacaaaatc
    39721 acgattgctt cttgcttgat ttcgttcttc gatcaattgt tcaatctctt catctaataa
    39781 ctcttctgct gtaaataaaa taccgaagat cgaaagccat tgcgagaata aagcttctcc
    39841 ttgtgccaag acagcggcag aaactttcgg ctggtctaga taagtgttca gccacttagc
    39901 aagctcatag accactgtga tcccatttgc tgcattaaaa tcatcgtcca tttcttcgat
    39961 gaatcgctgt tctaaatttt ctaacaaagc gattttatcc gcatcatctg ccaaattctc
    40021 tgaggcagtt tcttggcgga atttcaaatt gtcaaacgta ttcttcagtc gttgtaaatt
    40081 ggtggctgct tctttcatcg tcgtttcact gtagcggatc ggacgacggt attgtgtggt
    40141 cgccatgaag aaacgcaaga cttgtggatc aacgtttttg atcatttcat gtacagtgat
    40201 gaaattccct agagatttac tcattttttc atcgtcttcg ccaatcgtta catagccatt
    40261 gtgcatccag taattagcga attttttccc tgttttcgct tcactttgtg cgatttcatt
    40321 ttcatgatga ggaaattcta aatcttgccc gccaccatgg atatcgatcg tttctcctaa
    40381 atgttttgtt gccataacgg aacattcaat atgccaaccg ggacgtcctt tgccccatgg
    40441 tgaatcccaa gaaatctcgc caggttttgc ttgtttccat aaagcgaaat ctaatgggtc
    40501 ttctttttgc tgctgttctt cccctgtacg ctgacttgct ccgacttcca gttcatcgat
    40561 ggattggtca cttaatttcc cataattttc aaatttacgg gtacggtaat acacatcacc
    40621 tgaagattca taagcatagc ctttttcaac caacacttga ataaatgcta aaatatctgg
    40681 catatgatcc atgactcttg gatgcagtgt agcaggctcg acattcaacg ctttcgtatc
    40741 ttcttcaaat gctcggatga aacgatcagc aacttctggt gctgtaatac ctaattcgtt
    40801 tgctgtacgg atgattttat cgtctacatc cgtgaaattc gatacgaaat cgactttata
    40861 gcctcgatat tcaaaatagc gtcgaattgt atcgaaagca atggtgctac gagcatttcc
    40921 gatatggata taattataca cagtcggtcc gcagacatac ataccgacat gtcccttttg
    40981 tatagggatg aattcttctt tttttctggt caatgtattg tagattttta tcattctgat
    41041 ctcctcactt tttctccttt gattcgaacg acttttgctg gtactcctac cgctgtagcg
    41101 tcgtctggaa catctttcaa cacaacggca cctgcaccga ttttggcgtt ttttccgatc
    41161 gttaccggtc ctaatatctg cgcattcgca gagagcatgg ctccttgttt aacagttggg
    41221 tgtcttttac ccgtttcttt tcctgtgccg cctaatgtta cgccatggaa taaaatcaca
    41281 tcgtcctcga tttctgcggt ttcgccaatg acgattccca ttccatggtc gatgaacacg
    41341 ccagttccaa tcgttgctcc tggatggatc tcaacaccag ttaggaaacg ccagaattga
    41401 gcattgatct tcgccaataa aaagagccgg tgctgatata aaaaatgaga gaaacgatgc
    41461 caaaacaagg catgaaaacc agggtatgtc aaaacgatct cagctgtcga acgtgcagcc
    41521 ggatcgtttc tctttgctgc atcaatcgct cgggtaaacc aactcatgac tttccactcc
    41581 tttcgtaaga attttatgta ggaaatcatc ttcttataaa gaaaagaagc gccttttaaa
    41641 taagtttcct tatcaaaaga cgctttccgc gtggttccac ttttcttcat aatcaatgat
    41701 tatctctgac agctaacgcc tgttatacgt tcttggctac tttgtttcct ataaaacagt
    41761 ttttcctgtt ttctgtgttt cgccaagacc actcccagag gcatttcggc aaccattcat
    41821 tgataccttg caccacccgg tatctctctt gtaatgatca gctgcttact tttttctgtt
    41881 catcgtgttt agttcagtac ttgatttaaa tgtgctaatg atttttcacg accaagcaat
    41941 tcgatcgttt caggcaattc tggaccatgc atttgtcctg atacagctac acggattggc
    42001 atgaaaagat tttttccttt tacgccagtt tctttttgaa cagctttgat ggcagctttg
    42061 acagttggag catctactac ttccatttgt tctagctgtg ctttgaatgc gtggagaaca
    42121 gtaggaactg tttcaccagc aagtacttct tttgctgctt cattcaatac aggatgttca
    42181 ttgaaaaata gcgtagacag atcaacgatt tctgcagcat agctcatttg aggttgatac
    42241 aggctaacga tcttcttcac ccattcgatt gtcttttcat ctgggttttc tgctacacgt
    42301 ccatcagcga ttagataagg cagacacatg tcagtaaatg catctagatc cattgctttg
    42361 atgtaatggt tgtttaccca ttgcagtttt ttcgcatcga aagctgcggg tgatttactt
    42421 aggcgttttg gatcgaacat tttgatcaat tcttggtgtt cgaagatttc gtcttcgccc
    42481 acaggtgacc agcccaataa tgcaataaag ttgaacattg catctggtag atagcctaat
    42541 tcgcgatatt gttcgataaa ttgcaagatt gattcgtcac gtttgcttag ttttttacct
    42601 gtttcactgt tgatgatcaa tgtcatatgt ccgaaggttg gtggtgtcca gccaaatgct
    42661 tcatagatca tcagttgttt tggtgtattg gcaatatggt catctccacg taaaacgtga
    42721 gtgattttca tcaaatgatc atcgaccgca acagcaaaat tataagttgg cataccatcg
    42781 cgtttttgta tgacaaagtc gccaccgata ttatcggatt cgaacgaaat agcgcctttc
    42841 accatatctg taaatgcgta ttctgtattg cgaggcacac ggaaacgtac aacagattct
    42901 agaccttgtg cttctttttc tgcttgttct tcaggtgtca agttcgcaca tgttcctgcg
    42961 taatgaggca tttctcctct tgcgcgttgt gcttcccgtt ctgcttctaa ttcttcttct
    43021 gtgcaatagc atttgtatgc tcgattgcta gcaagcagct gatcaatcag cggttgatag
    43081 atttctttac gttcagattg acggtaaggg ccgtattctc caggattttc aggagattca
    43141 tcccaattca ttcccaacca agcaaggttt tccaactggc ttttttcgcc atcttcgata
    43201 ttacgttttt ggtcagtatc ctcaatacga ataataaagt ctccatcatt gtggcgggca
    43261 aacaaataat tgaataatgc agttcttgca tttccaatat gcaggtgccc agttggactt
    43321 ggtgcatagc gtacgcgtac tttcgtcatt ttcttcctct tctttcaaac tagactttga
    43381 ctaacagcaa aaattccgtc agttacggct tctcaaatat catttattta aagccactca
    43441 gcacaaattt taccttttct aaggtaaata gtaaagccaa ctggttgaat ttccttacca
    43501 gttggctgaa tttctttttt attttgattg tttagtcttt ttctctgtct cttttttatt
    43561 gtcgattcct cgtcctgcat gtgcaggttt agcaaagatc atgcgtccag cagctgtttg
    43621 aagggcactt gttacaacga cttcgatacg ttcattcatg taatgctgtc catcttctac
    43681 gacaaccatc gtgccgtcat caaggtaggc tactccttgt tggcgttctg tcccggcttt
    43741 tactactaga acattcatcg tttcaccagg gatgacgact ggttttacgg catttgccaa
    43801 cgcattgata ttaagaacag gtacattttg gaattcagag actttattca agttatagtc
    43861 attggttaca atgacaccat ctaaaagctt agccaatttg atcaatttgc tgtctacttc
    43921 actgatatct tcaaagtctc catcgtacat ttcgacagat ataccctctt ctttttgcaa
    43981 ggcatttagg atatctaatc cgcgacgacc acgcacacgt ttcaaactat cccctgagtc
    44041 cgcgatgtat tgtagttcgt aaagaacaaa attagggatc atcaaggttc cttctaagaa
    44101 tccagtcttt gtaatatcat aaatccgacc gtcaatgatc acgcttgtat ccaagatttt
    44161 atatttatgg aaattttctc ctgattttcg ttctaacagt tgactctcaa cttgactagc
    44221 tgtttcttgc tcgatttttt tgcttcgtga accaaaaact ttcttccatt cttcgattcg
    44281 tgtcgttccc attctcaatc ccagatagcc taagatgatc atcagtaaaa tcggcaatac
    44341 actattgacg aatggaattt ccatattgta aagaggggtc gagatcacaa cacctaaagt
    44401 caatccgatg atcgcaccga tcgcaccgaa gagaagataa gtcaagctga cctcacttag
    44461 tttttgttct acttgtttga taatagctga gatatgtttg gccaacagca aagataataa
    44521 aaagaaaata agtgcgccta acaggctgtt ggtaaatgta ttattcagcc atccgtttga
    44581 ctgttgaccg attgcctgcc aagctgaggg taaaaatgaa atacccaagc ttgcgccgac
    44641 aatgatcatc agcaaagtaa tgacacgttt ttgcatcgtt tttcctccgt ttcgacaatc
    44701 atttattagt tagcggaaca cacgcttcaa cgtttcagcc aaagttgcta ctccaactat
    44761 ctcgattcct tttggcgctt cccagttacc taagttgttt ttaggtaggt atacttttgt
    44821 gaagccgagt ttttggactt ctttgactcg ttgttcaata tgactgacac gtctgatttc
    44881 tccagtcagt ccaatttcac cgatgaaaca ttccgttggc tgtgttcctt tttctttata
    44941 gcttgaagca atactgacag cgatagctaa atcaatcgct ggttcattta attttacccc
    45001 acctgcagct tttaagtaag catcttgatt ctgaagaagc aacccagctc ttttttccag
    45061 cactgccatg atcagagata cccgattgaa atcaagacct gttgttgttc gtttggcatt
    45121 tccgaacata gtaggtgtga ctaatgcttg gatctctacc aaaattggac gtgtcccttc
    45181 cattgccaca acgatagcag agccagttgc accgtctagg cgttcttcta agaaaatctg
    45241 tgaaggatta gcgacttctt ccaatccatg ctcatgcatt tcaaaaatcc caatctcatt
    45301 cgttgagcca aatcggtttt ttactgctcg caaaatccga aaactatgat gttgttcacc
    45361 ttcaaaatat aacaccgtat ccaccatatg ttctaacatt cttgggccgg cgatcgagcc
    45421 ttctttggta acatgaccga cgataaaaat ggctatccca tttgttttag cgattttgag
    45481 tagttctgca gtggtttctc ttacttggct gacacttcct gccacacttg tgatgtctgg
    45541 ctgcgtcatc gtttggatgg aatcaatgat aacataatca ggggagatat gttcgatggc
    45601 acgactgatt tcgttcatat ctgtttcagc gtatagataa aattctgtgt tgatacttcc
    45661 gaggcgttcc gcgcgtaatt ttatctgctc tgcgctttct tcgccggata catataagac
    45721 ttttccgcct atggctgcta attgctgaga aacttgtaac agcaaagtgg atttcccgat
    45781 acctggatcg ccaccaatca agaccataga ccctggcacc acacctccgc ctaacacacg
    45841 gttcagttct tctaattttg ttttgatccg aggttctttt ttagggacga cgtctgcgat
    45901 ttttgttggt tttgcttttt ctcctgtcaa acttgtccga gttctgcggt ccgttgtatc
    45961 ttgttcgatt tcttcgacca tcgtattcca ttttccgcaa tttgggcagc gtcccaagaa
    46021 cttaggcgac acatacccgc aattttggca aacaaattgt acttttgatt tttttgccat
    46081 tgtaccgcct ccttttatcc taatcatgag acattaaatc aagcttaagt atctttaacg
    46141 ttttatttac tggaagaacc aaatccgcct tgacgttgag cagtcgtttg atcttgatct
    46201 gcttttaaaa acggcaaaaa gatcccttgg ccgatacgct cgcctttttt gatcactaca
    46261 tccttgaaac caaagttggt gaattggaac atgatatggc cttcatttgc ctcattattg
    46321 taataatcac tgtcaatcac acctactccg tttgtcaaca ctaaaaaccg cttcaacgga
    46381 ttgcttgaac ggttagcaag ctgcaaaaat tcatcttctc ccatgtaagc tttgattcca
    46441 gtaggcacga gggttggttt caattgtttt tcattttctg atacatcttt tttcaatcca
    46501 tctgcgactg tttccaaccc ctttttccag cctgctttcc agatacttgg aatcacgacc
    46561 tcttctgctg cttcaaaatc atagccagca gcatgatgtg tagcgcgctg cggtagattg
    46621 atattttgat ctttatattt agaaataact tcaaatcctc tttttctcac cggttttcct
    46681 ccctaaataa actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa
    46741 ttcttcaaga aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac
    46801 tgcaagttgt acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata
    46861 gaacgaaatt aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat
    46921 gcaaaacgaa ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc
    46981 aatcttgcgg caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag
    47041 gaagtgatct tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca
    47101 gaacgtccga ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg
    47161 atgaatatct ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg
    47221 acaagagacg tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag
    47281 cttttgatta tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa
    47341 ttagatcatc tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt
    47401 gtcgcggcag accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg
    47461 actacaaatc ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg
    47521 gcacaagcag gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa
    47581 gccgcttcta ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg
    47641 gcaattattt ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg
    47701 aaatgactga cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac
    47761 ttatgcctac aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga
    47821 atcaacaagc gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata
    47881 tcagtgaaac gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta
    47941 cagagatcga aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga
    48001 cgaacaccga cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg accggcatca
    48061 tttacgaatc tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga
    48121 atgccgttat ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat
    48181 tattgcagca agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg
    48241 atacttctca tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga
    48301 ttcctcgagg cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca
    48361 ttgaaacagg tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg
    48421 cagtgaacat catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa
    48481 cattgctgat ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg
    48541 tagaacacgg ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg
    48601 ctttagcaga agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag
    48661 tggtcgattc tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag
    48721 aaacgatcgt gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg
    48781 cggctgtcta tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg
    48841 cagagattgg tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat
    48901 taacttccac aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt
    48961 gaaacattat tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg
    49021 tcaatatgtt tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg
    49081 cttttctggg agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga
    49141 gtcaacggga aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac
    49201 gcttgcacat tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt
    49261 ttttcgatca ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat
    49321 cttataaata tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg
    49381 tacgataagt agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg
    49441 acaaagattt tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg
    49501 gtaaatctgt ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat
    49561 aacgaagcag ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt
    49621 cagccagtag ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt
    49681 ttctagtttc accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca
    49741 accgatcatc tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat
    49801 attcttccag catctattat ccccccattc tttttcttaa atgaatttta gcttacctac
    49861 tgaaaatttt ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca
    49921 catccccttt ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat
    49981 attcaacaaa catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga
    50041 cagtctcaca acctcttggt aaacggcgtt caaaagcttg catctgcggt aataagccca
    50101 accaaacgaa aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga
    50161 cagctttttc acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt
    50221 gataaatgat acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc
    50281 tttcaccaat atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa
    50341 agcggacaaa actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag
    50401 caatcgtcgt caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga
    50461 tcggtacaag gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata
    50521 agacaaatgc tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa
    50581 cgaaaatcga agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta
    50641 attcagaata gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat
    50701 tatataacaa tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac
    50761 cttcccaaag gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg
    50821 ctataaaacc gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac
    50881 tgcccaagac cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat
    50941 acatcatgat tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag
    51001 tagagacaaa taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga
    51061 tattcccaga taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca
    51121 atgttttttc aaaccgatcc gactagataa gaaacctgta aatagcatca ccacaccagt
    51181 gactaacatg taactagtaa ataacagggt cgtttcagct ggcgtagcgt ttaactgtgc
    51241 agcaatcgtt tttaagattg ggtctactag acccactccc ataaaagcga tcatacaggt
    51301 agctgctaca actagcgctg aacttttccc ctttgtttct tcatttgttt gttcttgtat
    51361 atctatttga ttctccaata tttccatccc tttctctcta ttagcatcat acatgattta
    51421 ttttacatgt aaatatgcac atgtaaaacg tggaaacttt actgtgatat aataaagaaa
    51481 ataagacgag aggtaggatt tttatgaata ctttagccta tatattatta tgcgtgcttt
    51541 tatccaaacc acgttctggg tatgaattga agcagctgat cacaattttt tgggaagccc
    51601 atcacagtca gatctataca acgcttgcca agttagacca gcagggttat gtgaccgttt
    51661 tggacaaaga cgagcactct caaaaaaaga tctaccactt gactgatgaa ggacgtgctc
    51721 ttgtagagga atggatcaaa gaggaaactc ccgctcctac gcaaaaagat gaatttctag
    51781 caaaaatcta tgctttttcg acgctcgata aaacaacagc tactggactg ctattcacta
    51841 gagaacaaca gctgaacaaa atcatgcaaa aaaatcaaaa aaaattgaat gacctctcgc
    51901 tagacaagaa acaagaattt ggacgttatg ttgtgatcca gcgcagaatt atgctttgtc
    51961 agcaggaact ccaatggtgt cagcaagtaa gagaacaaat gcagcagttt tttgagtaag
    52021 ggataaacag aacctttcta gaaatatcta ttgaagagca aattatcttc aataaaagag
    52081 caatacagtt ggaaaagaaa aagcgatccg aacgacaggc caaaacctat cgttcggatc
    52141 actatttttc tagaactgca ttcaagagac tatgacaaaa agcatgccaa ggcctttttt
    52201 caaataacag tgttccatca ttcattactt tgaattgctt ttagcacact tcttgaaagc
    52261 gggccaacga tgatcaactg caacggtaaa gcggcaatca cattcatttt ccaagcattc
    52321 atataagcag agaataattg atcaggtatt ccttgttcca tgatcacacc gaataaagac
    52381 atacatgtca ccattcctaa aaccatcaag catgaaatag ttaagatcat ttggaccttg
    52441 ctttctggat ttactggtag tttaaaggca atttttttcg ccagcacacc gatgatgaag
    52501 acatccaaaa tgaaagccac cacgaatcca ggcactagac caatcaacaa acttgataaa
    52561 tgaagatctt gatgcagcca taaattatac gcgctcatcc ccaagaccat caaaaaacac
    52621 atcattgttg taaaaattat tccttctttt ttatttgtag gcaatctgta ccactccttt
    52681 aaatttgtca cgaaaatcat cgtagcacat ttaaaaattt ctcgtaagta ctttttttat
    52741 tttctgccct tttatttcat aaacttcatc agccgtacgc tcgacaaagt aagaatcatg
    52801 agaagtaaac aaaatcgttc ccggatatat ttgcattagc ttctctaaag cttcgatcgt
    52861 tgccatatca ataaaattag tcggttcatc taacagcaac acattacttg gcttagtaaa
    52921 aagcaaagca atcgtcagac gggtcgcttc tcccccactt aatgtacata acggtttcgt
    52981 caaagtttcc tgagcaaacc ctaagtgatt cagcaaacta cggacaagcg attctgaaaa
    53041 ctctgtttgt ctcatgacaa aacgaatgat ggattcttca gatgtcattt gataaccctt
    53101 ttgctgatat acttgaaagc ttactttcgg agagagaagg attccttctc cttgtttgcg
    53161 gatatgttct aataaggttg tcttacctga accattttca ccgacaatcg cgattttttt
    53221 acctaaagaa aactgaaaat caccatttac cagtaatgtt ctcccacttc tttccaattg
    53281 gacattttgt cccatgatgg gatagcggct gtggagttca agagattttg gtactggaaa
    53341 acggatttgt ttcatctgct gcggcttggt tgtttctcct attttttgga gtcttctctc
    53401 taatgttttc gcattctttt ggatggcttt ttgtacactg tctttttgtt tagaggaaga
    53461 caaccgatcc ggtctgattt gttgcttttt ctttttactg gacacttttt ccaacttttc
    53521 cgcttgtttt ttcttttctt ggatcgattt ttccaatcgt tttttctctt tcatgatcga
    53581 ctctgcttct cgtagctgag tctttttctc caattccttt tgagtgagat aggcactata
    53641 attccccgaa aactctcgga tttctccgtc ttttacttcc cagattttcg atgctagctc
    53701 atctagaaaa tagcgatcat gactaacaaa aaccagcgta ccataataat aacgaagctc
    53761 ttcaatcagt cgatcaatac tttttctatc tagatgggtt gttggctcgt ctagaagcat
    53821 cccttgttca taagaagaaa gaagacgtat caactcgatc tttcgttctt cccctccgct
    53881 ccatttccgc tctgaagttt cttccagtct taactggaaa tgactggcta attccaaatt
    53941 taggtccttt gcttcagcga gataggttaa ttgaggcagg tagttgaagg tgatctctgt
    54001 ttggattttc ccatgatcgg gaaaaagttc accggctatt agtttgagca aagtcgattt
    54061 accgacacca ttttctccta tgatccctat acgatcttgc tgatagacac gaagagaatc
    54121 aatagatagt tctagctggt tgccaaagct gacttgtaga tttgttatat ttactgctaa
    54181 attttccata tgttttacac tcctatttca tgtaaaacaa caattatcga tactaaaaaa
    54241 gagcagaaaa atctgtttgc aaaaaaactg catcacatct tttcctactc tgaacgaaat
    54301 aactatgact aaatcaccaa atctgtcata gctttcacct attctactgt cgttcgaaaa
    54361 aatggacata ctccatccat gaaattgttg tttatacatg tcttttgaat ttatttagtt
    54421 gttattcaaa agttcgaaac gtaatttcat cgatgcagtc atttttccga cctccttttt
    54481 tattaggtta ccacacacga ttcaaaaaaa gcaatagcta ctgaatagta actgaaaacc
    54541 ttttattcga acgtagtgat cttttctggt gctaaacgaa cagactcgta accttcttct
    54601 accgcttttc ctattgataa aataataact ggaacataac ggtctttatc caaaccaaat
    54661 gcctctgcca actgatctgc ttcgaatcca ccaattggat ttgtttcata gccatgatca
    54721 cgcgcaacta acatgaattg catagccgct aagctagaat cgactttgac tacatcgttc
    54781 atttcttctc ttgaaaaact cttgtaataa ggaatgatcg cagctaattg ttgatctctg
    54841 acttcttttg gcatcttccc tgattcgtaa gcttggttat agatttcttc gccatattcg
    54901 taacattgca tatcgccaaa aatcatgacc attgcagaag aagtatcatt ttgtctcgta
    54961 ttgaaacgga tcaaaggacg caagatttct tttgcttcct cgctttcagc gaccacaaag
    55021 cgccatggtt gcatattgac agaagaaggt gcagtcgtcg cttctttgat catttccagc
    55081 atttcttcgt gtgggatttt gtatgtttca tcatatacgc gaacggatct tctaccagtc
    55141 ataattgttg aaaaatcatt tttttgttcc attattatta acctctttcg tttctgttgt
    55201 tatattgcaa agttgggtat tcaatttatc taaaagtgtt agaaattgtt tcatttctac
    55261 atcgctgaaa gccggggata gcacggaacc tgtatcttta tggtccattt cacaatgctc
    55321 gattttcttc tgggcttttt ctgttagcgc tacatacact tcccggttgt tctgaggatt
    55381 tctctgacgc gaaacatagc catttgcttc caataccttc agatgacgag taactgctcc
    55441 ttgatcaatc tgcaactttt cttgcaaact gctttgtagc aatggatttt cttctttcag
    55501 tctcatcatg atctcgtatc tagtaagact tataccgata ttttcttcaa agatctggac
    55561 gattttttga tcggctaatt tgatttgata taaaaggtga ctaatctctt ctaaagacat
    55621 aatttcctcc tttctaataa ttgactcgtc aacgattgac ttgtcaataa tagaataaaa
    55681 aaagaaaaaa gtcaaagaat cagctgtgta ataaaaaaaa cgctctcaat agaaaagtat
    55741 agagaaaaga aattttttgt agaatgaacg gaaaaaatga taaagaaagc acaacgctaa
    55801 aaccaagatt attgattctt atcaaactat agaagatagc tcttctgcag tagaaaattt
    55861 tacaccgcca aaaatttccg aaatcgcctt ttgcaagaat cccgtcgaat attagaaatt
    55921 ggatcagtat gcttaaacag taataaaaaa gttatgacaa gatgttgtca caaccttttt
    55981 tgagtaaacg gtgttcaaaa acttgcatct gcggtaataa gttcaaccaa acaaaaaaca
    56041 tgaaaatctg tttttgtttg gctgttctta ctacttgatg cagggcagct ttttcactac
    56101 ctctttttaa taaccataaa atcctattcc ttcatatctc cagcccatcg gtgttagcca
    56161 atctctttcc ttagcactca aggtatagtg atgggtacca gttttttcat ttggattaaa
    56221 tagtcgataa attggcttgg aaccaccaga ataaaaactt agaccttcat aacgccaacc
    56281 gatttttgtt aaagagttga tctcagattg tgccattgtg taatgatggt ctccactgta
    56341 aggattgtat aaacggtaga caggttgtcc gctgtttggt gcttgccaag caatcccttc
    56401 atattcccag cctacagttg gcagataatt tttttctccg gcattcaatg tatataaatg
    56461 ttctcctgta tgacggttat acagacgata catcggagaa gttgaagttg gtgctgtatc
    56521 atactgtgtc agattatagg tttcgattac attgttcaat gtagaaccat aattaggcgc
    56581 agtggcataa cgacctgtta gccaagccgt agcatctttg taagagttcg tattgctttt
    56641 ccatgcaccg gaatagtagt aaacgcctgg ctgaaaagaa gtcgtcttca ataccgttac
    56701 gttatcttgc aatgattctt tgtacgaagg atattttcta aaagcggcat tcaccgtaat
    56761 ccattgccca ttcacatatt ccgaagtagg catcgtaaca gattggccat tatagcttcc
    56821 tttaatccca aatagattgt aattaggggc agaagccaaa gtactgttcc cccagccact
    56881 ttcaacgatg gcttgagcaa tcatgactga tgcatataag tcattggcac ttgccaaact
    56941 ttgtgcagtt gaggcaatac tatcaatgaa cgcttgttga gaattagcag atgagcgagc
    57001 ataaaaagca tagccagtat agttttcgaa ttcagacaaa tctgcttgat gggtatgtgt
    57061 caatgtttgc ccatctgtca attggttcgt gatttcttct gcattctctg gattgttctg
    57121 agcttctgct gatgtactgt cttcggtttg tgtttgttct gttgtttcag ttgttggttc
    57181 ttcggttgaa attgtatttt ctgagacagt agctgactgc aaagtactct ctgtatcgtt
    57241 ttcaattcct gtttctgaag aactcgatgt ctgagttgcg acagtttctg tcgattcttc
    57301 agctagcact gttgtgttgg caactgctag atttcctaaa agaataacgc taaataataa
    57361 attctttgct ttcatctttt ttctcctttg aattatgaaa cttttcctaa ataacagtgt
    57421 aactaaaaaa gtaaaagcaa gtcaatgtcc atatttaaat aacatttgtt tctttttgat
    57481 aaatttgctt tttctacata aaaagacgta aatgaagaat attcttctcc attcacgcct
    57541 taaattgatg gtcgtttgaa taactattta acaagtaatt ctctttggat attatccgtt
    57601 atggattata cttcctgtgt cgtcggacgg ataatcatgt tactgacaga catccggtct
    57661 ggcgtttcta tcgcaaatgc tacagcagaa gctatatctt cggaggtcaa tccccattct
    57721 ttttgcgctt catgaagttc aagagcagcc tttttgtcgc tgatggtttg atataattca
    57781 gtttgaacag ctcctggaga gatgatcgta gatttgatgt tattttgccg ttgctcttga
    57841 cgtaatcctt ccatgattgc acggacagca aattttgttc cgcaataaac ggcggaatca
    57901 ggataaacaa catgtcccgc cacagaatca gttgaaatga tatgcccaga tttttgtttt
    57961 tccatgattg gaagtacggc ggaaattcca tttaacacac ccatgatgtt gatatcaagc
    58021 atattttgcc attcatcacg gtgaccttca actaatggag cggttggcat gattcctgca
    58081 ttattgtaca atacatcgat tcgaccgaac ttttccatcg ctaaatcgat aactgcttgg
    58141 acttgtgcaa aatctcttac atctgctgtt tgaatatata gctcagcttc tggtaaagat
    58201 tcttttattg cttttaggcg atcttctcgg cgtgcagcta taactagttt agcccctttt
    58261 tctgctaata gcttcgttgt tgcttcaccg attccgcttg aagcgcccat gatgacaatc
    58321 actttttctg ataaatcatt cattctattc tcctcctgtt ttgaaaaatc tcttgcttat
    58381 actcactatc tcttatttca tcttactgtt caaataatcc gttcgacagg tttgacgatt
    58441 ttggcaggga ttcctgcaac gatggtattg tctgggacat ctttcgtaac agttgcattc
    58501 gctgcaacaa tggcattctc gccaatcgtt acgcctggca gaatagttgc tcctgctccg
    58561 atccaagcat ttttcttgat gtggattggc tttactgaca agcctcttcg ttcttttgga
    58621 tcggttagat ggttcaccgt gatgaggttg acacgaggac caatcaatac tgaatcttct
    58681 aaacagatgc cacctaagtc aacgaacatc acttgacgat tgataaagac atttttccct
    58741 atactaatat gtttgccgaa atctgtataa aaaggcagca gaacagtcac cgtatcatct
    58801 actttgtcac taatgatatt ttctagccgt ttgcggatat ctgctggttc atggtaccca
    58861 gtattcagcg ctgctaaagg ctcttcgttt tcagcttgga tctgatggat cacttcaaag
    58921 agcggatccg ttactcgaat ctctttattg acgatttgtt tttcgatgag gcttgtcatt
    58981 ttgctccctc cttttatcct tactttataa cggacggctt atcatgtcta atacttataa
    59041 aacatactta actattcctt ttaactatac ttagtggtat gcttttttaa aaacaggagg
    59101 gaaaaaatgg atattcgtat actgacgtat tttttagcag tagctagaga aaaaaatatc
    59161 agccgagcag caaaatctct gcatatcacg caacctactc tctcaaaaca actgaaagat
    59221 ttagaagaag aacttggtgt gacacttttc actcggggct cccgagagat acagttgacg
    59281 gattctggtc gatatcttta ttcaaaagga aaagaaatct tgtcgttggt agaaaaaacg
    59341 gcgaacaatt taacaaatga cgcaatcatc tctggagaaa tctacattgg cggaggcgaa
    59401 actaaagcaa tctctttcat ttctgatagc cttcattcgt tgatcaaggc gcatcctgat
    59461 atccagtttc atttatatag cgggaatgca gatgatatca tagacaaact ggatagcgga
    59521 ttacttgatt ttggactcgt aatcgaaccg acaaacaaac aaaaatacca gtcatttcga
    59581 ctgccagccc aagatcgttg gggacttttg atacatcgaa atcatccttt agcaaagcaa
    59641 accgtcatcc atccgcagga actgaaaact gttcctttga tcatttcccg gcaaacgtca
    59701 gtggacagtc aactagctga atggctaggt ttcagcatag atcagttatc gatcgtcgga
    59761 acatataact tattgtacaa tgcttctttg atggtcgata acgggacttt tggtgcatta
    59821 gctctagatg ggatcatcaa tacttatggg acggaaaaaa tattcatccc actcgatccg
    59881 ccattacaag cttcactgaa tctcatttgg aaaaaagacc agcctctttc tcctgctgct
    59941 cgtaaatttt tagatgtcct aacggagaaa tacagttaag tagagttttt ttccttcgct
    60001 ttcagctttt taacaacgaa taagataagc aaaagatcca acgattaaat aacataagtt
    60061 tcttaattta tttcttcctt ttatttctga taattccagt aaattactat aattataaaa
    60121 aaagaattga caaaacataa ctgtctatat aaaattgatt acgttaacaa taattcgttt
    60181 catgtgacta gatcatacta ggcatgaaaa atctctgagg aacatggttt ccctaggagg
    60241 tttttcgtgt ctatttttgt ttggccgcaa tcaactggtc tagcaaacgt gaaaaccaaa
    60301 tcttcaaaaa gggtgcgcgc atgaaaatca aaaagatctt taatcaaaat gcagtattgg
    60361 tagatgacgg tggacaggaa aaagtagcta ttggaaaagg gattggattt gacagaaaaa
    60421 gaaatgattt gatttttgat cgtgatgtcg aacggatatt tgtcatggaa ccagaaggtc
    60481 aaatcaagtt gcaaaattta cttaatcaga ttgatgaacg ctttttattt gcagcagaaa
    60541 cgatcattga tcatgcggaa actgttttga tggaaaagtt gaatgaacat gtattgatcg
    60601 ctttgaccga ccacattgct ttttctgcgg agaacattaa taacggtatc gtcatccgca
    60661 acaaactttt aagagaaatc gaagtgcttt acagtgatga attcagtatt gctcaatggg
    60721 cagtagagta tctgacaaaa gagcttggga ttccatatac ttatgacgaa gcaggatata
    60781 tcgcaatcca tattcacagc ggacgaagcg gtcggaacaa taaccatcgc agtatccgtg
    60841 aagtgacgat tgtatctgac atcatccatt tagtggaatc agaattggaa acagatatcc
    60901 attcagaacc attttcactg aattattcaa gactggtgaa tcatttacga ctgcttctac
    60961 aacgaaccca tgcgcaacaa tatgctgttt tagacacgga aatcgtagaa atggtcaaac
    61021 gaaaataccc agaaagctat aagatttcta aaaaaatccg tgtgttgctg accaaagaat
    61081 accagctcgc gatcacgaaa gaagaactgg gttacttggc catccatatc gagcgtttaa
    61141 gaagcgcaat cgtacaaaca aatgttaaca atcatgagga ggaaaaaaat tgaaagcata
    61201 tctacaacga atgggacgtt cccttcaact tccagttgcg gtcttacctg ctgctgcttt
    61261 actagtaggg atcggaaact ggtgggcatc ttatagtaac gacattgtcg ctcatttttt
    61321 acaagctggc ggtaacgcag ttttaggtca attacctttg ctgtttgccg ttgggttagc
    61381 actagggatg tcagtggaca aatcaggtgc tgctgccttg gcaggtttgg tcgcatttga
    61441 agttccagta aatgttttaa aaactgattc agtagctact ttattgaata tcaaaccaga
    61501 atcagttgat ccatcttttg ctcagatcag taacgtattt attggtatta ttgccggatt
    61561 gattgcggca gctctttata atcgtttcca cgaagcaaaa ttaccgatgg ctttatcttt
    61621 ctttagcggc aagcgtctag tgccaatctt ggctgccttt gtcatgttgc tgatcagtgc
    61681 agtgctactg ctcgtatggc ctccggttta ctctgcttta gtttcttttg ctaaattcat
    61741 tttgagttta ggctggggtg gtgcaggtct ttatggcttc ttcaaccgtt tactgatccc
    61801 tacaggattg catcaagcaa tgaactccgt gttctggttt gacgtagcag gaatcaatga
    61861 tattggtaaa tttttggctt ctgaaggaac aaaaggcgta acaggaatgt atcaagctgg
    61921 atttttccca atcatgatgt tcgggcttcc agcaggagca tatgctattt acagaaatgc
    61981 acgtccagaa aacaaagcaa aaacagcttc cttgatgcta gcagcagctt ttgcttcatt
    62041 ctttactggt gtgacagaac cacttgaatt ttcatttatg ttcgttgctt ggccattata
    62101 tgtcttgcat gcaattttca ctgggatttc tttagcagtg agtgcattct tccattggac
    62161 agcaggattt gcctttagtg ccggatttgt cgattacttc ctatctttga aaaatccgat
    62221 tgcgaatcag ccattgatgt tgatcgtcca aggattagta acagcagtga tttactactt
    62281 tggctttaac tttgctatca aaaaattcca tctgatgact cctggccgcg aggaagcaga
    62341 tctttcagat gacgatactg caacgactaa ttcagacaat aaatatgcgg cacaagcaag
    62401 caaaatctac gctgcattag gcggagccga caatgtaact tcgatcgata actgtactac
    62461 acgccttcgt ttgcaagtca aagatacagg cctgatcgat caaaataaaa tcaaagccac
    62521 tggcgtacct ggtatgaaga tcatcgacgg aaaaaatgcg caagtcatcg taggtacaga
    62581 agtacaattc gttgcagatg agatggcaaa actacatggt ggtgctgcag cacgacctgc
    62641 acaaacaaac accgtggtta aaacagaaac atttgccggt gaaacgatcg aacaagatat
    62701 ttacgctatc gcaaatggga aactgattcc aatcactgaa gtttcagatg atgtattcgc
    62761 ggaaaaaatg atgggtgacg gctatgctgt attaccagaa aatggagaaa tcttctctcc
    62821 aatcgctggt acgatcacaa acattttccc aacgaaacat gctgtcggaa tccaaacaga
    62881 tgctggtatc gaagtcttgc ttcatatggg catcaacact gttgacttaa aaggcgaacc
    62941 attcacttta tatgtagaag aaggtcaaaa agtcgcaaga ggtcaattga ttgctttagt
    63001 tgatcttgcc gctattcaat ctgcaggcaa aaacacggat atggtcgttg tattcacaaa
    63061 tggcgacaaa gtccaaagct tagaaatcga acccgcacgt gatgtaaaag caaatgataa
    63121 aatcggctct gtttccaaca aagcctgagc catcgtcttc cccagacttt tttatcgata
    63181 aacaaacagg agctgagaca aaaatcttgt ctcagctcct ttttccgaat aaaaacggcg
    63241 ttctcctgcc aactccgcgg taataagtcc agctaaacga aacatatgaa gagctatttt
    63301 ttatttgact cttcttatta cttgatgcag aacagctttt tcacaaaatt tttcttcata
    63361 ctcaaaacta atcggctttt tcacaacctc tttatacatt attcctgatc caatatttct
    63421 tttaatgctg cttcaagaga agttaatggt tttcccaata ctttttctaa atcatcagag
    63481 gaaatagcta actgatcctt tttaatatca tgttggatcg ctaaaaacat gtcagctgct
    63541 tcttgaggga atccggcttc ttttagattt tcgacaaacg ctgcatcact tgcttcaata
    63601 atttctaact ctttacctgt cgcacgctct aatgccttgg ctaattcttc atagtcagtt
    63661 agattgccgg ataattctaa gatttctgga gagtcacttt ctactagtac ttttgctgca
    63721 gcttctgcat attcacgttt caatgcccaa cctgtttttc catttcctgc agcataaacg
    63781 aattgaccag tttttaaagc ctgcccaatg ataggtaatt cattctctac ataccaatta
    63841 ttccgtaaaa atgtatggga aatacctgaa tccttaatca ttttttcagt gaattggtga
    63901 tctggcgcta atacactgat cgagtgatcg gcatctgcaa aacttgtata agcgatataa
    63961 gataccccag ctgcttgtgc tgccttcact acattcttat gttcggcttg acgatttcct
    64021 ggaacgccag agacaaaaag caatcgatct attcctttca acgcttcttt cattgagtct
    64081 aaatcatcat aatcaccaat acgtatatta aatccagctt ctttcagtgc ttctcctttt
    64141 tcttcactac gtactagtac atagatttca ttttgtggaa ccatcttttt taagaattcc
    64201 agcgcataac caccaaaacc tccagttgct cctgttacta aataatgcat atttgccact
    64261 ccctattctt tttatttgtt aagatttaca gctgtacatt tataaattac acctttatca
    64321 taaagagtgt ggtaagataa gtcaagcaaa aaaaatgaaa ggatgacaac tgatgaagta
    64381 ttctctgcaa tttagtgatg caatccatgt acttgcctat attgaaatgt ttcaagatac
    64441 ccgatcactt tctagtgaga tgattgccag tagtatcgaa acaaatgcag caaatgtacg
    64501 taaaatcatg agtcagctca aaaaatccgg tttgatcttt acaaaaaccg gaaaaccgca
    64561 gccttccctt gccaaaagtc cagatcagat ttctttacta gatgtctatc gcagcatcga
    64621 aggtaataca cagcttatcc aagtcgatcc aaaaacaaat ccaaattgcg tcgttggtgc
    64681 gaatatccaa gaagctttag aagaaagcta cgctcggctg cagaaaaagg cagaagaaga
    64741 aatggctaag atcacacttg atagtctgat acataaaata gccgttttgg aaaaagaaaa
    64801 aagaccagaa aacacaggcc tcgtagagag atttttataa ttctctctac tgtgcctgca
    64861 tttttctgat cggaatcgtc tacataaatt cttttattct ccgggggaca aaagccttgt
    64921 catgcccctt tttcctctct aatgctcagg tcaaagcgct gaagtcatga cgtcttgata
    64981 aacggtgttc aagtagctga ccctcggcat tagttctgaa aaatgcaaaa tatggaaagc
    65041 tatttcccat ttttcctctc taatgctcag gtcaaagcgc tactttcacg acctctatat
    65101 tatcatggtt tattgactac cggtatccag atttctccca tatatttccc gttggtaact
    65161 cccatttcga ctacagcatt tggaccccca acataggcgt aatcttctac ttctcttaaa
    65221 acatgcccaa aggccgtatt cgtcaaatca tttcttaatt cttcaggcga ttctgctaaa
    65281 gcgcttacaa caagatacgt tccttttggg aatgggatga ttcgcgtagt gtttggaaca
    65341 tcctgactcg tttgaacacc gacatagtac atcattttcc cgttataggg ttcgttgact
    65401 gtaaagaaat aatgattttc agcagcttgt ttcaatgcat ctattgttcc attaccgata
    65461 actttttgct caaatgcttg tttctctgca gccaaaccga caaaatccgt attgctactc
    65521 ttcaattcca caccaatacc cgtcaccgta aaatctggtt tttcttcgat cctatacttt
    65581 gccatttctc ccaccctttc tattatatta agttcatctt acataaaatt aaagggaaat
    65641 gacacgatta cgctcttttt tctagggata tttttatttt ttaactattt ttttgctttc
    65701 actgcaatcc aaatctcact gtaaacatta ctgaaatcct ctccccattt tgtaaaagag
    65761 atctcgggag cttcgactaa cccataatct gaagaaggaa gccattctgc atagattttc
    65821 ccccaagtat cttgtaaagt ttggggaaat ggccctttat taggaaaaat ggcccaagtg
    65881 tgttcttcta tagcaagcat ttctaaatca gcgtacggat tttcttttgt cgtagcaaag
    65941 ccaatcatgt gcatcaaact gcccttttct tccaagcggt ctccatcaaa tccataagaa
    66001 acgttgatta cttgatttgg atataaatct cctaacttat gcatctcttc cctttgagtc
    66061 tccgtgatcg tctgcgctaa cttttgtatt tcttgattga ctccttcaaa tttgacagga
    66121 acttttttcg ccacacctgc cacataaaac tgttctttct tttcgatacg aaattccatc
    66181 gattttcctc ctctgacatc aataaaaaat gtcaagcgtg gaaaagattt ttgtatgccg
    66241 tatctactga tttctgaagg cagatatcct gaccaatcac gaaaagctct tgaaaaacct
    66301 tcgactgttt catatccata tctaaacgcc gtttctgtca ctcttgatcc atctagaaga
    66361 tcgcgattag ccaaagccaa tctacggttt ttgatatagt ccattagcga catccctgca
    66421 ataaacgtaa agatgcgttt taattgatat tcagagattc ctgtattttc ggctattatt
    66481 ttataggaaa gtccttctgt caaatgttct tcgatgtaat cgatcaattg gttcagatct
    66541 ttaagcatat ttatcttcct ccttgattgc ctttttatca tattatcaaa gaaagtcaat
    66601 tacttgatga atcaggaacc aatttagtca gatgtagaaa atgttgtatg gatctaaaga
    66661 aaattctcaa gcagcgtttc gtaacggata attggtatta tctgcctatc tatgttttac
    66721 ctttagaaaa ataaattttg gggaagacag attctaagta aacaaagcgg taaaaatatc
    66781 ttttattccc ttagttaatc cgcttactac ctactgtaca aagcaaattg ttgttcaaac
    66841 tgctgatcag aacgctgctt ggatttttct ttgcgataca tcatacctct tttatcaatt
    66901 ggaacaatta tttatttcta acaaaatcac ttcatttttg tcagataaat aaaaaacaaa
    66961 aaaagttcca tctttaaaac acgctattct tttacttttt ctcacattct ctaagaaaag
    67021 aaaaggagaa tgaatttttg acaattcgag caaaagtgat tatcaaatat gtaaaatcag
    67081 ctactttttt cttaaacgtt tctttatact aaatataaat aagtgtttca tgaaaggaga
    67141 actagatgaa taattggctt gagtattttc ctgaaaacgt tttggaacgc ggttacagct
    67201 atcatctgca cggttttgtc cgtcatttga actatacaag caagtacttg tccgcaacag
    67261 tttctggcac ggaagattat aaggtagtga tcacttggga tgaaaagacc aacatgactt
    67321 gtgattgtct atatgccata gaaggaaaaa aatgcaaaca catggctgct gtcttattcg
    67381 cttacgaaga acgtccaata aaaaaatcca actactcact ttccgaactc tcgtcccttg
    67441 tttcctccgc tagttcctct cttgtacggg agcttttaac agagattctt atagaacatc
    67501 cgcactttat cgaacgcttc aaagttaaga tgccatttca tgcaataaat tattcagata
    67561 aattaacaac tatcatccat aaatacgatc atattatcaa gaaaaacaaa aacaggaaaa
    67621 cagcgaagtt catcatggag atgcgaaagt tcatccaaga agcagttgaa tcgcttattc
    67681 agcaaaatgc ctatcttccc gcattcgaat tgatcaatga agtgattgct actctcgaaa
    67741 cattctattg ggagcctgag gatgaacgaa cccttcttct gatagaggac tgttattatt
    67801 tatggaaaga attattagca gaagcacctc atgcagaaaa aaggcaaatg ttctcttggt
    67861 tcgtctgcca agtcgatcat actgacgcct cttattcaaa acgctactct ataaaaatcc
    67921 tgaaagaaga ctttcgggaa aaagaatttt ctaaccaaaa gaaaaaaata gataaaaaac
    67981 taaagaaacg gtaaagaaag acgatttcaa tgaaaaatag caagtaaacg ataacaacca
    68041 tcctaaatcc acaagaaata cagcagacct tgctttaaaa acacgaatca tgcaaaggat
    68101 gaagtaaaga agacttaacg cgtacccaat attagtcatt tgactattag gtacgcgttt
    68161 tgttttataa gtcatgacaa atttccttcg ttgctggata gattttccgg cattgttgat
    68221 agattttatg atacttttct acattttgta agagaggttc cgctgcatct gtatattgga
    68281 taaaattctg acaacaactt tcaggtgtgt cgaaccaccc taaccctagt gcagcaatca
    68341 ttgttgcgcc taatgcaggt ccttcctcca cacttaaaca atagaccgtt gcatcgaaaa
    68401 tatcagcttg gatttgtagc catgcaagat ttttcgcgcc tcctcccacc gaaacaatac
    68461 gtgtaaatct gcgacccgct agttctttac gtttcaatcc caccattgac aaatgctcat
    68521 taaaaattta gaatattcac aagtttaacg acaattatga aaatttatgt cgtgctaaag
    68581 gagttttgtt attggatgaa aagttagata taaaaactgc gatcaaagac acgttaccta
    68641 ccgttttcgg ttatatcggt attggacttg catttggtat cgttgggaaa gctgccggat
    68701 ttcatccact agtcgtcacg ttgatgtccc tgctggtcta tgctggttct gcccaattta
    68761 tcacagtcag catgcttgct agtcacagcc cattgctttc catcgttttc tcgacctttc
    68821 tagtcaattc ccgaatgatt ctgatgagta tgacgattgc tccttatttc aagaaaaatc
    68881 ggctgcttca aaatctgctg attggcacat tgctgacaga tgaaagtttc gcactgggaa
    68941 tgaataaatt gaactatact ggacaaaagt taaactttcg ttggatgaat acagctaatt
    69001 ggatttctta tttgacgtgg gtgggttctt ctcttgttgg cgcactttta gggaatttta
    69061 ttactgatcc gaaaaagttt ggtttggatt tcgccatcgt cgctatgttt atcgggttac
    69121 tttatcttca agtcatttct gatagaaaca cctctaaacg attacagttg atcctgattg
    69181 gtctcacttt gattctggta tatgtcggac tgatttttat cccaagcaac ttagtgattg
    69241 tagttgtaac actgattggg tgcggattgg gggtgtggat caaacatgcc ttcttttgag
    69301 tttacattgc tgactatttt aggttgtact ttagcaactt ggctttctag ggtcctacca
    69361 tttgtcttac tgaaaaaatt cgatttaccg caaccgttgc ttgagtattt aagttttgtc
    69421 ccgatcgtca ttatgtctgc tttatggttc agcagtctat ttacccaaaa tatcggacat
    69481 ctgccgcaga tcaatctcga aaatgctttg gcttcagttc ctacactttt agctgctatt
    69541 ctttcaaaaa gcttattggt gatcgtcttg gcaggaattc tttccctttc gttgattcgc
    69601 ttattataaa taaaaaaaca agagcttatg gcaaattact gtcatgcgct cttgttttta
    69661 tcaaagtcaa ataagaaaaa aatcggcgta ttcttgctta tccgttgaac ggaccactaa
    69721 caacgacttt tcctaacata tgtcctgttt ctacgtcttt ggttgcttgt ttcaagttct
    69781 tcgcattcat cccatctgaa tagaccttgc tcaatgtact gcggataatc ccttcatttg
    69841 ctaaagcagc aatgtgcgct aaagcttctc cttgcgtttc gatttcatag ttatagtctg
    69901 ttttagcgaa catatactcc cagtcgaagc tgatagattg gtttttccaa acacctatat
    69961 ctaacggctc tttgatccct acgatcgttc ccacatgtcc aaatggtcgg acaaggtgct
    70021 tgatttgatc aaagtagcgg gtaatatcga ataatacagc gacatactct acttgatcga
    70081 ttcctaattc acgaaaactt ttacttaagt cgtgatgata atcgattgga tagtctacac
    70141 ctgttttttt cagccattca aaattcttcg gacttgcagt cgcgtagact tctaggccag
    70201 cccaatgcgc tagttgattc aaaatcgaac cgacgccccc ggatccgttg atcactaaga
    70261 tttttttccc gcgattggca ttttcttctg gagttagacc aaatttttca aataacaatt
    70321 cataagcggt caatgaggtc aacggcaagg ctgcagcttc ttcatctgaa agattttttg
    70381 gtgcaagagc gacaatacgt tcgtcaacta gttggtattc ttgattgctt cctgctcttg
    70441 ttgttgtacc tgcgtaaaaa acgcgatcgc cgacagaaaa tttttgcact tcgtcaccta
    70501 cagcaacaac tttcccgact ccatcaaaac ctaagatacg taaagcattt cttatcccgt
    70561 tatcttggcg aagtttcgta tcgacaggat taacagatac tgcgttgact tttaccagaa
    70621 gatcccgtga actgggtaca ggtatctgtt ctttttcatc aataaagctt tttggatcat
    70681 cgatcggcaa cccttcataa aaaccgacta ccttcattaa ttccttttct gacatacgaa
    70741 ttcctcctta gcttcatcaa gttggagcat ccatacagtg ttcatttcaa tatatatagt
    70801 atgctatttg ttccaccttt tttattcatc ataattattc tcttacttaa agtaaactat
    70861 ttgcactttt ttggttaaaa aagaggttgc gacaagactt ttgtcacaac ctcttataaa
    70921 cggtgttctc ctaccaactc ttcggtcaca agtcaaaata ttcatcaaac atgagaagct
    70981 gttttttgaa attgctcctt gtgactcaga gttaaacggc aggctcacaa cctctgatat
    71041 aaacggtgtt caatcaactg accctcggta ttagctcaaa aaacgacaaa atatgaagag
    71101 ctatttttgt ttggctgttc ttattacttg atacaggaca gctttttcac gacctctaat
    71161 tcacggtgtt caagcaagaa atcattgttc actgaaaagc gttggatgtt ctttttcaaa
    71221 ggtctctaag atctctcgta cttcatcagt agatttcgta ttcatcaact gaactcgcaa
    71281 gtcatttgct cctggaaatc ctttgatata gattttaaag aaacgatgta gtcctgtgat
    71341 ggaacgcggg atgatttctg aataatgatc ttgcagatct agttgcagac gaagtagtcc
    71401 gatcagttct ttttctgtat gttccctcgg gtctttttca aatgcataag gattcttgaa
    71461 aatccctcgg ccaatcatga tgccatcaac gccatatttc tcggccaatt cttctcctgt
    71521 ctggcgatcg gggatatctc cattgatcgt gattagtgtt tgtggagcga tttggtcacg
    71581 taaagcaatg attttaggaa tgacttccca atgtgcatct actttactca tctctttacg
    71641 cgtacgcaaa tggacagaaa gattggctat atcttgatga agtaggtgac tgatccattc
    71701 ttccatctca gacatttccg tatagccgat acgcgtcttg acgctgacag gcaaaccgcc
    71761 agctttagct gcttcaatca attgtgccgc tacttctgga cgaaggatca agccactgcc
    71821 ttttccacgt tctgctacat tagggactgg gcagcccatg ttgatatcga ttccttggaa
    71881 gcccatttct gccatagcaa tgctcatttc tctaaaaaat tctggcttgt ctccccaaat
    71941 atgggcaacc atcggctgtt catcttctgt aaatgttaat cttcctcgta cactgtcttt
    72001 tccatctgga tgacagaaac tgtctgagtt tgtaaactca gtaaagaaga catccggtgc
    72061 tgctgcatgc tttacgacat ggcgaaatac aacatctgtt acatcttcca ttggtgctaa
    72121 aacaaaaaaa ggctttggca gctctttcca aaaattagta gtcatttatt tgttactctc
    72181 cattctatca agcagtgaag tcgcaatctt tctcaaaaac attgtcatta tactaggaaa
    72241 caaaaaaaga agcaaagtga acagcttaaa agatgtttct gattgagcaa aaaaaagact
    72301 gtgacaaaaa tcttatcaca gtccattttc cggaataaat ggtgttcaaa agcttgcatt
    72361 tgcggtaata agtccaacca aacgaaaaat aggaggagct atttttgttt ggctgttctt
    72421 attacttgat gcagaacagc tttttcacaa cctctccact aaacggtgtt caatcagctg
    72481 accctcggta ttcgctcaaa aaacaacaaa atatgaggaa ctattttccc gtttgtttct
    72541 ctaatactca ggtcaaaacg ctgatttcac aacctctttt ctaacttctc aatcctcttc
    72601 ctttattgat caaataccaa gtaacggcgt acaaaacaac gatgaataac acaagtacga
    72661 tcatcgatgt tgctactggc acatcgatcg ttccaaggaa tccgtaacgg aaaccggaga
    72721 tcatataaac gatcggattg acttttgaaa cagcttgcca aaagggcggt aacatagaga
    72781 tggcatagaa aacgccacct agataagtta acggctgcaa gacaaatgta ggcacgatcg
    72841 atacatcatc ataagactga gcaaaaatcc cgttgagcag tcctgctaat gaaaacaaga
    72901 ttgccgtcat caataatgta atgatgacca tgaaccatga atacacatga agtggtacaa
    72961 agaacaagga aatgatcgtt acgagagagc cgacgagaat actgcgaccc acaccaccaa
    73021 tcacaaagcc ccaaataatc acatgcgtag gaacaggtgc taccaagatt tcttcgatat
    73081 ttttctgaaa tttttgagag aagaaggacg aagaaacatt ggcatacgaa ctagtgatag
    73141 cagacatcat aatcaaacca ggaacgatga attccatata tgaaaaacca cccatgtcac
    73201 cgatccgccc accgatcatt tttccgaaaa taacaaaata caaggatgtc gtgatcactg
    73261 gcggaaccag tgtttggacc catatacgca aatagcggtt cgtttccttg atagctaaac
    73321 tttttaatgc agtaaaataa agattaaaca tttttctcct ccgtatgatt attttcttct
    73381 gtgattttca agaacagttc ttctaaacga ttcgacttat tcctcataga aagaactttg
    73441 attccttgtc ttgtaagctg atcgaagatt ccgttcaccc cttgatttcg ttcaacttcg
    73501 acagcgattg tctgctcatc ttcaaatgta caagcatatc cttcaatcac aggtttggta
    73561 tcatatggcg ctaaatcaaa gataaatgtt tcaaattgca gttttgacag aagctctttc
    73621 atacttgtat tctcgatcag ttcacccgat tggatgattc cgatgttgcg acaaagcatt
    73681 tcagcttctt ctagataatg cgtcgtcaaa atgatcgttg taccattttc gttcaattct
    73741 cgtagaaact cccacatctc gcgtctcaac tcgatgtcaa ctcctgctgt tggttcatcc
    73801 aagatcagca aacgtggttc atgcatcaat gccctagcaa tcatcagtct tcgcttcatt
    73861 cccccggaaa gcatacgtgc acggacattt ctcttttccc aaagattgga ctgcttcaaa
    73921 tatttttcgc tgcgtttcat cgcttctttg cgagagacac cgtagtagcc tgcttgattc
    73981 acgacgattt gctggaccgt ttcaaaaggg ttaaaattga actcttgtgg aaccaatcca
    74041 atctgttgct ttgctcgaac tagatccttg tccagatcat aaccgaagac attcactttt
    74101 ccagatgttt tattgactaa agaagtaata atcccgatcg ttgtagactt cccagctcca
    74161 tttggcccta atagcgcata gaaatctcct tctttcaccg aaagatcgat tccccgcaat
    74221 gcttcgaccc cacttccata gatctttttc aaattattta tttcaagcgc atttgccatt
    74281 tatttcctat ctccttatag acatgattct taattgtatc atggaataca gcagatgaaa
    74341 aaacaacttg gcttccacac aaaaacacac ctgctaacaa agaggagatg tgttttctag
    74401 ttcatgatca tcttcgatag aataactatc taaacaactc tggaactttt aaaatcataa
    74461 aagtaccgac agcaactact acgatgacat atagaagatc cttataactg aggttcttgt
    74521 cttttttatt catgttaagc tttcctccgt ccttttatcg aaagcttttt tcttttattt
    74581 taaccgaaat aaataaaatg gaaatagtta ttttcaattt ctgttttcgc tgtttgtttt
    74641 acggagaact ttgccatgga acgtgcgtct cgtcgctgat gctaagtttt ttcgataaaa
    74701 gctgctagaa ggaagacagc gtggtttatt ttgtatcgtc ttttgctaaa tcttccaaga
    74761 tcatctggac agcagactcg ttcccattga attctgccac ttttttggca tcaatgtact
    74821 tcttttgata atcgtctttt tggacccacg catcatgctt cgtttgataa agttaatcaa
    74881 acggatacca ctgacgctgt tcatcgaaca acaaactttc tttttgggcc tgttcaacta
    74941 tggatttagg ataattcaaa actttcagca aggcaatcgc atttcgggtc cttgaaggtc
    75001 cttcttttaa tttgaaatca aaactgattc cttttccttc tgttacttgt tcttcaaagt
    75061 ggacattctc acaataattc tttaaaatct ctgtcaattc gatatcatgt gtggcgacaa
    75121 atgctaaaga tggatactcc gcaagccacc gaacgactga agcggaagaa gcaatccgtt
    75181 cgatcgtatt tgttcctttt aaaatctcat ctacgaagca gtagcagcgt tctttggaag
    75241 ccacttggtc cagcaaacgt ttgatcgatt tgatttctga aacaaaataa ctgtcgcctt
    75301 caaataaatc atcttcgatt gccatggaag tcaacacatg tccaggttgc agggtaaact
    75361 gttctgcaat cgctgtttgg atcgtctgtg ctagaataca gctgatggct atacttttga
    75421 catacgtaga ttttcctgat gcattggaac ccgtgactaa tgtattcttc tgccagttga
    75481 ctggattaac taccgcttct tttaacaatg gatgatagat atctttcgct gtcacaccgc
    75541 cctcagtaaa ctctggtcgg caagtgatcg gcatataggt gcgataattc aaaacggctg
    75601 ctgcaacttc caattctccc agcaattccc acagcttgat ggcttcagtt gaatgctttt
    75661 ctaatttagt gagaacgaaa tgataggaga taaatggcag catgaacatg atgttgacat
    75721 attcaaaaat caattctgct tcactcccgc tcttcattcg aaatgaaaaa cctaatttag
    75781 ggatgtctct taatggttta gtttgctgtt tcagctcttt ttgaagtggt gtcgtcattt
    75841 ttgctaactg attggcggct gaaatcgttg aaaccaaata actcatgcta ttcagctctg
    75901 tttctagtgt ttgtttcttc actagataat agaccgtatt gaatactaag ccaccgattg
    75961 ctagatagat tcctagtact tgccctaaaa gaagcaaacc tacacccacc agcggaagca
    76021 agccaagaaa aataaataaa cctacgcgtc caatcttttt actttttcca tccgctagat
    76081 attgttttgc aaaattatga tctttttttc ctaaacgagc gaattgatat tggattcttt
    76141 ctctcagttg aggattttcc tgataaaaag caattagttt ttctaactga taatcttctc
    76201 caaaatcaaa gctacgtaaa cgctgataca acgcttctga acctacacta gaataggtac
    76261 tgttgatccc ttcaaatact tcgaacatat ccaagtcata ccaagtaaga tcgtcgattt
    76321 cactatccca tgaacggaat tttttctccg tgagccacgc atccttcaaa ctttcttctt
    76381 tatcaaatct cggttgataa ggaatttttc cccaattact acgaactgtt tcctttactt
    76441 tcaaacgtga aaaaatgtct aaaacaacta ataaaataac aactgcaata ataattaaaa
    76501 cgataaattc tctttggccc atatccctct ccgttccttg atttgttagt tttagtatag
    76561 cactgttgcg ctatttgaaa agggggtaca aaaataactg tcttcgttac ctaattcgta
    76621 tcaatcacac caaaataaaa acaccttcca aaaaagaaga tgtctttaaa acgagataca
    76681 attttttgtc attcacttca ggtaaattag agccaattaa accctccgca acttgacgga
    76741 gatggagaag atgcccccgt ctaatctagc ggtcatctct cctcccattc cttccaccaa
    76801 tttttttgac aggtaaagcc cgattccgct agattccgta tttgtgcggc tggtattttc
    76861 ggtataaaaa cgctggaaga ctttttcaac agaggtttgc gctcctggtt tgataggatt
    76921 ggccactgtt aatcgaatga atgaatcctc ttttgaaaat gtcacctttg cttgtccatc
    76981 tgcataacgc aataaattct gtatgatatt ttgcagcact cgcagcaaca tgagcggatc
    77041 tgctaataca gttgtattta tcgagtgttc agcgaacgct acctcgatat ttttctcaga
    77101 aaattgttca taaaaagaga aaaatacttc ttccacctct ttagaaaaag aaagcgtatg
    77161 gagatccatt tctttttgct tcgtctctat tcgagcaaga tcataaaact gatccgtcat
    77221 actgagtaat cttgcaacag attgtttgat gatcgttaaa tacttctccc gtttctccgt
    77281 atcctctacc tgttccagta attgtacata accgttaata gcagtcaaag gagttctgag
    77341 atcatgagaa aggccgacga tcgatagtct gatgttttct tccatttctt gaaatgcttg
    77401 tcctttttgt tcattttcat ccacccatgt attcagtgca tctactaggt ccattagatt
    77461 cttttctcga aattcgactg aaagacgacc accatattct tgtcgttcag aaagagtggc
    77521 gattttctta gttaatctct tgatttccct ttgcaggaag aaaataaaac ttcccataaa
    77581 gataacaacg agaagcaaca gccacttaat caaatctaaa ccccactccc cacaccgtat
    77641 gaatatacac agcatgtgcc tttgctagtt tccctcgaag attggaaata tgcatattca
    77701 ctgttttctc actatcaaaa tatggttctt gccaaactgt ttcataaaga tttgctcgcg
    77761 aaaatacttt tttcggatgt tctaaaaaaa gcttcagcag atcgaattct cttcctgtca
    77821 aatggacagc cacaccattc acatttacat ccctcgactc tttgtccagt aagatttcct
    77881 tgtaatagat gcgttgcgac agatctttta cttggacaga agtctgatga cgaagttgga
    77941 tgccgattcg taacaacagt tcttcgatat caaatggttt cgtcacatag tcatccgctc
    78001 ctgctgccaa caaggaaact ttatctcttt gatcgctttt tgctgaaaga acgatgatcg
    78061 gaacattcga tgtctgtcgg atttctgcta acaatgcttc acctgaaagc cctggaagca
    78121 tgagatctag aaggatcaaa tcgacaggat atgtgcttaa caataatttt ccttccgtac
    78181 ctgaataagc atcaagaagt ttgtatctct gttcaagaac ctcctccagt aaagaatgaa
    78241 cagcttcatc gtcttcgata atcaaaatag tttccagcag gaacacctac ttaatgtcag
    78301 atttttggaa aataaacatt cctagagctg atgataccac gattgttact agactgacca
    78361 gaatcatttt cggtgcttgc gaataagttg cttgttgcgc aaagtccgta tacatgagcc
    78421 acgtttccgt aaaccactcc gttagaaatt ccaagccttt gatttttgcg aaaacaccgg
    78481 gaacgaaggc cgttcccatg atataaagaa aagaaagaag caaactgaca ctggtacttc
    78541 tagtgatgaa tgtcaaagcc actggaacag ataatgtcgc taatatcagc agaagcaata
    78601 caggaaatac ttttagaaca taatttccat agtttttgat tccctcaact tcaccaaatc
    78661 cgaaagcaag tgtgtaagca atcatggcta gccccgtaat caaaaccacg ccaagtgttg
    78721 tgatcaaaag cgtaacgaga agtttcgaca cataataagt cattcgattc cgtcctaaag
    78781 ataacgtatt gcgaatcgta ccgttttgaa attcttcccc aatataaaag cacgcaaatg
    78841 ctgcgaaagc cagaagaatc catgcagcca ttttttccac cattaaaatc cctgttgcac
    78901 cgttgatgac tgtatccgtt gcttggttta attcattgcc gtattttgta tgcatatacc
    78961 attcgttcca agaataaaaa agcggaagta cgaccatcag tcctagtgag acccacattt
    79021 tacggctctt ccatactttt attttttcag cagttaatag attaaacatg tggattgcct
    79081 ccagttaatt gtaagaaata atcttccagt ttttgcccaa ctaggtggat accatccaca
    79141 ggaacttttg cctctaccaa tgcttgattg atggctgcta cttggtcaat tccttcaaaa
    79201 agctggataa cacggctaga ttggacaaaa taatccctat aaccaagaga gtcgagaacc
    79261 gttactgctt gttccgtcgc cgaagtgtca atttttataa attgacgaga ttcttgtgcc
    79321 aattcttctt tcgataattc tttgatcaag tgtccttcat gcaaaatacc gtagtgagtg
    79381 gcgatctgtg acaattcatc tagcaagtgg ctagaaagca agacggtgat gccccgttct
    79441 tttaccaaac gttggatgat ttctcgcatt tctgccatcc ctgctggatc catcccattc
    79501 gtcggttcat ccaaaatcaa aaattctgga ttcgtgatca gtgcatttgc aatagctaaa
    79561 cgctggcgca tacctaaaga gaaattcttt gctttctttt tccctgtatt ttccaatcgc
    79621 atcattttca gcaggtcttc gatttctcta tccgacacac cgccgttcgc tgcttggata
    79681 cgtaggttat ctcttgcagt taattctgga taaagagcag gggtttcaat cgattgaccc
    79741 atacgtcgtc ttgctgcctg caaatccgtc gttccaaata attcgatcga accttcatca
    79801 atcgagatca aacccataat agttctcata aaggtagact tacccgctcc attctcccca
    79861 atcaaaccat agatcattcc acgtttgatt tcgatcgata ctttatctaa tgctttggca
    79921 gcaccgtatt ttttggttat accagttgct ttcaagaccg tttcatttcc aaaatccata
    79981 cttatccttc tttcttattg ataagtgaag tatagttgac gggtataaag aaagcagcca
    80041 gaaatgtata aagaaagtgt aaattttcca attgaatttc aagaaaaatc agactgtctc
    80101 gcagacgtgg ttgagttgtc tgccaatcaa agagcgcacc aaacagtgct aatccaagtg
    80161 gactggccac catggtaatc gttgttatcg ttgacatcag acgtccttgt atatgcgttg
    80221 gaacttctcg ctgaagccag accatataaa aagcaaaaga aaacattaat ccttaaaaac
    80281 ggacttatga aaaataatac atgccgtttg ggatttacaa ttagctacca aataagaggt
    80341 tatgacaaaa gacttttcac aatcccttct tccgaataaa cggtgttctc ctgccaactc
    80401 ctcggtcaca agtcacaata tttatcaaac atgagaagca atttttgtaa attccttctt
    80461 atttcttgaa gctgaccact tctgtcacaa cctcagaagc ggataaataa tattgtttga
    80521 tgatacttta tctttttccc gtttgtttat agatcgaatg atattataga ccaagaatcc
    80581 tacctattaa catagctaaa aacacaaaaa aggcactttc caaattgtgg aaagtgccag
    80641 aaaagttagg attaaggtaa ctgcgctcag cattgcttcg cccagtacga ttctcgtaac
    80701 tcgtgaactc gaacgataat cgcaattaac gttttgagaa ctgtgaagct ttacgggctt
    80761 tcttaagacc tggttttttc ttcgtatcat tgaataagtt tgaaaagcct tgatttataa
    80821 aggtttttcc gtaaatatat tttgttatat ttgattaaat ttgaaatgtt tgtgttctaa
    80881 aatgtgttct aatttaaagg cagtatattt cgtattttat attaaatttt taggctcatt
    80941 cattctcaaa tccattctat ttagtcaata aatattttaa aaagacttta taaacaaaga
    81001 tgttcaagcg agcatcttat ttttttgtgc ctaatttgaa aggagaacat ttcatgtata
    81061 aaaaacaaaa aaagaaatta tgtgtattaa ctattttgag tttggctgtt caatcaattg
    81121 taggagcaat tgctcctacg attgcttttg ctgatgaaat tacccatcca caaacggtaa
    81181 ccgtagaact agatttagca catcaatatg ctgttgaagg aacttttagt gatggtcgtc
    81241 caatgtcaga agtcactgtt ccgcattatg cagtgtacaa cggcgttaaa caagatattt
    81301 tctgtgttga accaggagtc ccgatttaca atgagttcac tcctggatat gagaagaacc
    81361 cgttaccaga tatgtccgat aaagccaaat tagtttccgt tttatggaaa aaagtaggta
    81421 cagatgcaga tactcatatt gtggcacaga agatgatctg gcaagaagtt aatgggtata
    81481 cccttcactc aataaaacga tcagatggta gtgccgtaaa tatcgcagcc attgaggcaa
    81541 aaatcaatca agccattgcc gattatcaga agaaaccaag cttccataat agtacggcta
    81601 aaacagtatt agggcaatcg actactgtga cggatacgaa taatctgaat ttgtcagagt
    81661 tcgatgaggt tgtggaaaat accgcaaaca ttgattatcg tgtgaatggc aatcagttga
    81721 taatcagccc aaatgccaat tcaaaagaaa gtggtgtgtt gacgcttaag aaatctgctg
    81781 gtaccggaac acctgtcgcc tataaaaagg ctggccaaca gactttaatg gccggggcga
    81841 tcgataagcc gaacacttat acggtcaaaa ttgatgtgga aatggagggt tctttgaaga
    81901 ttaaaaaagt tgataaagaa tcgggtgata ttataccagg aacggttttc catttagact
    81961 ttggaaaaac tttacccgca aaagacgtga ccaccgataa agaaggcatt gcgaccttag
    82021 atgagattcc tcatggtaca aaggtaacca ttactgaaaa atcggtgccg gcgccttata
    82081 cgattgatac cacacccatg actgccacaa ttaaagcagg tgaaaccatt tcagtgactt
    82141 caaaaaatac gcgagaaaaa ggtcaaatta ttctggataa aaccggaatt gaaacaggga
    82201 gtgatctttg gaacgataat tactctttag cgggcaatac gtttgccatt cgcaaggata
    82261 gcccaactgg tgaaatcgtc caagaaatga ccacggatga aaaaggtcat gcagaaacac
    82321 caaaagagat tgccaatgcg ttggaattgg gaacctacta tgtgactgaa accaaggcta
    82381 gtcatggctt tgtgaatact ttcaaaccag taaaagtcga attgaattat gtcaatcaaa
    82441 cagtggctct tgttaccagt aacgtaaaag ggcaaaacca agaaattact ggtgaaacca
    82501 ctttgacaaa agaagacaaa gataccggtc atgaaactca agggaaagct gagtttaaaa
    82561 gagctgaata caccttgttt actgttaagg atggaaaagc ggtcaaatgg accgagaact
    82621 ttaaaccaga attgttgaag ggaacgaaag cttctgatga aaccgtaact ttgactttag
    82681 atgaaaagaa tcaagttgcc gttaaacact tagcgattaa tgagtattac tggcaagaaa
    82741 ccaaagcgcc tgaaggatat accttggatg aaacaaagta tcctgtatcc atcaaaaagg
    82801 tggatgatag cgaaaaaaat gctgtgatta ctcgagatgt tgcggcaaaa gaacaagtga
    82861 ttcgctttgg ctttgatttc tttaaatttg ctggatcaac tactggcact gccgaaactg
    82921 gcttcaatga tctaaccttt aaagtgtcgc cattggaagg aaccaatgaa atcaccggtg
    82981 ccgaagatga agcgaccaca gcttataacg agcaattagg gtttgatggt tatggcaagt
    83041 ttgaaaatct tccttatggg gattatttac ttgaagaagt agaagcccca gaaggttttc
    83101 aaaagattac gccattagaa atccgttcta cttttaaaga aaataaagag gattatacca
    83161 agagtgatta tatctttact attaccgaaa aagggcaaaa gcaaccgatt aaaacggtaa
    83221 cggtccctta tgggaatcta accaatgaga atttctcagt cagtttgaac cgtttgatgc
    83281 tttatgattt gcctgaggaa gaagatagct taacttctct tgcgacttgg aaagaaggag
    83341 ataaagaatt gactactctt gattctaccg agctagtcga taaattgagc tataatctac
    83401 atgaaatcaa agaagactgg tatatcgtag ctcaagccat cgatgtagat gctacaaaag
    83461 ctgcccaaga aaaagataaa aaagccaaac cggtggcgat tgcggaaaca accgcaactt
    83521 tagcgaataa agagaaaaca ggaacttggg agattctgca taaattaact gtcgaacaag
    83581 ttttagacaa gaccattgtc ttattcaact atgtgtatga aagcaaagaa gcctatgaag
    83641 ctggtgatga gccggtagcg aaggatgcta gcttgaacaa tcaagcgcaa actgttaatt
    83701 gtatggtgga acgtcatgtt tccatccaaa ccaaagcgca cttagaagaa ggttctcaga
    83761 cctttactca tggtgacgtg gtagatatgt ttgatgatgt atccattacc catgatgtac
    83821 tagatggctc aaaagaagct tttgaaacaa ttctgtacgc tttatttcca gatggtacaa
    83881 gtaaagaaat ttggaaatct ggcaagattg attacgaagt gaatgacaaa gaatttacca
    83941 aaaccgtact tgcggaaaaa gtggatacaa gtaaatatcc agaaggtaca agttttacct
    84001 tcaaagaaat taactatgac aaagatggga atatcaatgg aaaacacaat gaagatttga
    84061 aagaaaaatc tcaaacttta acgcccaaaa aagttccgac aacaccaagt acaccagaac
    84121 aatctgaaat gccaactgtt ccaagtggct ctcaagaatc tagtcctaca gtgaaggcat
    84181 tccctcaaac cggggagaaa acttccaatg tgctactgtt cattggcttt agcttgatct
    84241 ttgcgacggc tggctattat ttttggaatc gtcggaacta aggtgataag atgaaacatg
    84301 taaatagtag aaaacaatac gcgcgaccgc ctcctaaaaa agtggtcgcg ctatttttgt
    84361 attcaaaact aacaatgaaa agaggaaaaa atatggaatt gaaatttgtt gtaccagata
    84421 tggtcgagac gttcggaaaa ctaacatacg gtggtgaagg tgaagttcta tcggaagggt
    84481 atggtcgaaa tactacaatt gttggtcgaa gttaccatct gttttcaaat aaacaacggg
    84541 ccgatgatat tgaagtggtg gtagctgcgg aagccggcga aaaggatttt gaccaagatc
    84601 aaccactaaa agctgtaaat ccccacttaa ttgccaaagg ctatgaaatt gagaatcgtg
    84661 ggtttaccga ttacgtgttg tatgttgatg atttagtgaa agcataggag gaaaagaaga
    84721 tgagattaac agaaggtatt gtcgtagatt caggattaac ctttggcaag ttgcgttttt
    84781 ccgcattacg tcgagaagta cgcaagcaaa atgaggacgg aacggttagt aacgaggtaa
    84841 aagaacggac ctataatttg aaatcctccg cgcaaggtcg aatgatccaa gtcagtattc
    84901 cagcaaatgt acccttacgc gagtttgctt atgatgcaga ggtagagctg gtcaatccaa
    84961 tcgtggatac ggtcgccaat tatgtttttc gggaaggaac caccgttaat tggtttatca
    85021 aggcagatga tttagtcttg aaacgacaac tgaatcaagg aaaacctacg aaccaaaatg
    85081 aaggaaagaa ataggtgaag aatatggaaa ttaatgttga acatatttta gattgtctcg
    85141 atcaatttgg caaaggtgag ctaacggagg aacaactcac taacgcatta acctatgatg
    85201 aaaaaatgtt tctgattatg catcaagggt tactggaagt aggtaacgat gccaaagaag
    85261 actttgatgt actggattgg ttaggggaag aagagtcttt ctttatggtt gtcgaggtaa
    85321 atgaaagttt atgtcggcag gcagaatctg ttttggaaga aatcgatgtg gagatgccgg
    85381 atgccatcga aagattttta aaacaattag tggaaacgaa acaacttccc gtagtagtaa
    85441 atgactagca taactttttg aaattgccca atgaaatcct attgggcttt ttcgactgga
    85501 ggtggaaacc atcaaaatgt acaaaggaca tcgcatacgc gcaggagatc aacacttgat
    85561 ttatcacttt gttcttggct ggctaagtgc cttattcatc ggttggatgg gcgtctttta
    85621 ttttccagag cttagacaat ttggtctttc taaggtatca ttttccacta ttgaaatcgc
    85681 ttggtccatg aaagatttag tatgtctgct tggaagtctc gctctttcag gaagtatgat
    85741 gttgctttat attcattttt ttcctgatca ttggcgaagt ttgtggaatc gacaaaagct
    85801 agccagaatg attctagaaa accattggta cgaagtgaag caaactcaaa gtgatggctt
    85861 tttcaaagat ttgaatagga gtcgaaccaa agagactatc agttacttcc ccaaagttta
    85921 ttatcgaatg aaagatggct tactgtctat tcgcgttcaa atttcactgg gaaaatatca
    85981 agatcagcta ttgaagttgg aaaagaagtt agaaagcgga ctgtattgtg agttagtaga
    86041 aaaggaactc aaagactcct atgtggaata caccttgtta tacgatatga ttgccaatcg
    86101 aattgggata gaagaagtag tggcagaaaa tgggacgttg cgattgatga aaaatcaagt
    86161 atgggcgtat gattccttgc ctcatatgtt aatcgctggt ggcacaggtg gtgggaagac
    86221 ctatttctta ctcaccatta ttcaagcact attgaagtca gatgcagaat tgtttatcct
    86281 cgatcccaaa aatgcggacc tcgccgattt aggtacggta atgcctcatg tctattctca
    86341 aaaagaagaa atttctaaat gtgtggaaga tttttacgaa cgcatgatgg ctcgtagtag
    86401 gtcaatgaaa gaaatgccca actacaaaac aggagagaat tatgcgtatc tcggacttcc
    86461 gcccaacttt ttaatctttg atgaatacgt ggcttatatg ggtgcgaatc gttttcctac
    86521 aagcatcgaa tagatgtgaa atggaagcct attgagatta tcaatagtga caactaatgt
    86581 tttgagagat ggaatgaaag ccttcaagtt gagctgaaac atccccgcta atactcctac
    86641 atgctctaat caatgaagta gattggtttt ggtatgaagc taggtgaagt cgtctgaaat
    86701 ctgcctaagt gcttgagcat aaggctgact atagggcggg cggctgaaaa agataactat
    86761 ggtaagaatg tctgaaaatg actgacgaat ctgcgaatgt acgggtctaa aaggctgatc
    86821 ttattgaaag ataggaacat acggacgtat ggtgagatac cgacaagtaa ggttccatga
    86881 tatgaaaatc gggatagcta acgaagaagc tataatttca caggctcata gtagatacct
    86941 aaggttatac atgatagcta aaaacattgg aacgatgtaa gcagaaaact atcaattaaa
    87001 tgactacttc tagataggtg catataagta ataacgaaat tgctttgttt ctgtgaaagt
    87061 aagggcacag taccgatgaa gcgtctaaca aatgtggagg gatagcccta agtctattta
    87121 ataacaatga atgttgaaac agatgaagtt caaggatctg gtaagaacgt gggaaaatcc
    87181 gttatggagg tgccacgctt gaacacaaaa atacgttact gggaatatta tgatctacaa
    87241 gaaacattcg atcagctatt tactcaaagt agaaacggga aaaagtttta tcaattatat
    87301 gagttaatca tttctgaaaa taatattctt ttagcatacc gtactatcaa ggcaaacaag
    87361 gggtcttcga caccaggaac ggattccttc acaattgata actataagga aatgaaccaa
    87421 gcagagttta tccatcttat tctcagtcat ttagaaaatt acaaaccaaa atccataaag
    87481 cgagtaatga tacctaaacc caatggtgag aaacgcccat taggaattcc atgcatgatt
    87541 gatcgaatga tccaacagat gttcaagcag atactggagc caatatgtga agctaagttt
    87601 tatgaacata gctacggttt cagaccattg agaagtgcaa agcatgctct aggtcgcatt
    87661 atgtatctca taaacatcag taaaatgcac tatgcagtag acattgatat caaaggtttc
    87721 tttgataatg taaaccaccg tttactgata aaacaattat ggaatatagg aatttgtgac
    87781 aaacaagtct tagctattct ttcaaaatct ctgaaatcac ccattcaagg agaaggtatc
    87841 ccgagcaaag gaacgatcca aggaggtatc atttctccac tattatctaa tgttgttcta
    87901 aacgacttag accattgggt ctcaaagcag tggcatacct ttgaaacaaa gtatccctat
    87961 actaaaggtt acaataaatt tcgtgctcta agagatacaa atctaaagca aggctatatt
    88021 gtcaggtacg cagatgactt taaaatcatg actaatgatt atccgacagc attaaaatgg
    88081 ttccacgctg tcaagcttta cctaaaagac agacttaaat tagatatttc caatgagaag
    88141 tctaaaatcg tcaatttgcg taaatgtaag tcggaatttt taggattcgc tatttgcgtg
    88201 aaacaaaaag ggaaaaaatg ggtttgtaat tcacgtattt ctaacaaaaa aaaggatcag
    88261 attaaagaag agattaaaca aagaatcaaa gatattcaaa agagtcctac agcacaaaat
    88321 gctctcctgt ttaatgctct cgtgctcggt gttcataatt atttcaggta tgccactcat
    88381 attaatttag atttgaatag gatgaactac gttctttcat tcactactta caaccgatta
    88441 tcaaattgta gtacaagatg ttaccccaac aaagcttccc caacttacaa aaagttttac
    88501 tcaacaacga tgaaaacgta taaagtagct ggagtctatt tgtaccctct ttgcgacgta
    88561 tctacaaaaa caatttacgg atttaatacg gaggatacac cgtttactcc gtttggtcgt
    88621 caaagattgg agcacaaaag cctacagtct cttgtgttcc aagaattaag aaaattgatg
    88681 gaatcgaaaa taccgaatag aacggttgag tatttagata gtagaatatc tcgttactca
    88741 atgaaatcag gcaagtgtga gattacaaaa caatttttgc cagcaaaagc cgttcactgt
    88801 catcactatt tgccaaaaag tcttggtgga gacgacaaat tcgataactt gagaattatt
    88861 cataaggata tccatcttct gatccataca acaaataaaa tgataatcga tcactacgtg
    88921 catgaactga aactggtgcc agagcaaata gcaaaaatca acctttatcg aaagatgtgt
    88981 aatctgcaaa acattcaata gaaaataaat agatggaacg ccgtatgagg tgaaagtctc
    89041 acgtacggtg tggagcgggg gaaaagatgg agattatatc aaattcttac ctatcgctat
    89101 agatgttaac gacaaaagaa agtgcggtga ttttgaataa gcttaaacaa atcgtgatgt
    89161 taggccgtca atctggtttc tttctgattc tagcttgtca aaggccggat gcgaaatatc
    89221 taggagatgg gattcgcgat cagtttaact tccgtgtggc tttgggtcgt atgagtgagc
    89281 ttgggtattc catgatgttt ggagaagtcg ataaaaattt ttttatgaaa cgcatcaaag
    89341 gacgaggtta tgtagataca gggggcagtg tgattagtga gttttatacg ccacttgtac
    89401 caaaagggta tgatttctta agggaaatta gcaacattgt tggattaacc gttcatacgg
    89461 aaagagaaaa taatagtgtg tagcctagga atatcaataa tcctgggctt ttttattgag
    89521 gtgatttatg acatttttag atttattcgc cgggattggt ggctttcgct taggtatgga
    89581 acaagccggt caccagtgta tcggtttttg tgaaattgat gaatttgctc ggaggagtta
    89641 taaagcaatc catgatacaa ggaaggaggt ggaaatgcat gacatcacaa gtgtatcaga
    89701 cgaatttgtt cgatccctcg gaccagtgga catcctttgc ggcggctttc cgtgccaagc
    89761 tttttcaatt gcgggaaagc ggcaaggatt ttccgatact cgaggtactt tattctttga
    89821 aatcgctcgg ttcgccactc ttctccaacc taagtttcta ttccttgaga acgtccgggg
    89881 attgctcaat cacgaaggag gggctacgtt cgagacgatc ctccgaacgt tggatggatt
    89941 ggggtatgat gtggaatggc aagtgcttaa ctcaaaggcc tacgttcccc aaagccgtga
    90001 gcggatcttc cttatcggac attctcgaga tgcatgtacc gaacaagtat ttcctatcat
    90061 tggatcgtct tcaacatctg atcaaaacat cagaaacttg gtaaatatca atccttccaa
    90121 tcgaggaatg ggtggccaag tctatggttc agatagcgta gcaccaacct tgaccggtga
    90181 tgaaggaatt aaaattgcct taccggtaaa ggatgggatt tctgtagcgg gtatgttgtc
    90241 gggaaatttt gaacaaggca ataggattta tgaggtaacc ggaacagccc ccactttgtc
    90301 aacgaaacaa gggggaacga agattatgat tcggaaaaat gaaacagaat atcaagaggt
    90361 gaagccaggg gacagtgtga acctggcttt tcctaattcc acaagtcgcc gaggtcgtct
    90421 gggaaaacaa agtgtgcata ccttactaac aggagaccaa caagcggtag tcacggatca
    90481 gtaccagatt cgaaagttaa cacctaggga gtgctggcgg cttcagggct ttccagattg
    90541 ggcttttgat cgagcttctc aagtcaattc agatagtcag ttgtataaac aggccggaaa
    90601 ctcagtaaca gttccggtca tttttgatat tgctagaaga ttaaaggggg taaaagcata
    90661 tgatttatga cgagttgatt ggagaaatct actgggtgat tggcaagatt caggcagatc
    90721 cgaaattgga agaagaactg taccggctca attttgatat tcgcaaaaac ggggtgaaag
    90781 tccaggtgat tcgtatgtag tggatgagga aacggatgct cgaatggagc tcaatcaagt
    90841 catcggtgaa ttggaacgaa ttgcggacct tgccaaagaa cctgatatcc gccaatactt
    90901 gtttgagata aaagcagagt tggaaattga gggtatcacc gcggagtaat agggaaataa
    90961 cccactgata ggcactgacc gcgggggatt tgcgaagcag aaatcctccg tggtgctgtg
    91021 cctgttcatg gacggcggca gccggccatg atgtctcacc ccccgtatct aacagggggg
    91081 tacaaaaaca caaaaaacaa gcaagtaacc ataaaaaatg cttgttgtat caaggatatt
    91141 aagtaatttg tataggtgtt aactcatttg gaaaagttaa caccttgaat ttggaggtga
    91201 aaacttggcg caacgaaatt tagattatcg gttattaaaa gaacgacgca acgaatatgg
    91261 cgtttcacaa aataaattag ctactgcttg cggacttagc cgcccatacc taaatcagat
    91321 agaaaacggt ggcgtaatcg cctctacaaa gaccatgaga aaaatttttg atcaactgga
    91381 aagtttcaat cccgatttac ctttaacgtt actatttgac tatgtaagga ttcgctttcc
    91441 cacaacggat gtgcggaaaa ttattcaaga aattctccat ttgaaattcg agtatatgct
    91501 tcatgaagat tacgcctttt actcctatca agaacaatat gtcatgggcg atattgtagt
    91561 gatgttatct catgaagaag ataaaggtgt ccttttggaa ttaaaaggtc gtggttgccg
    91621 gcagtttgaa acttttttac tcgcccaaaa gcgtagctgg tacgactttt ttgaagattg
    91681 tctaaaagcc ggtggcgtga tgaaacgctt ggatttagca atcaatgatc gagtggggct
    91741 actggatatt cctgatttaa cgaaaaaatg tcagaaggaa gaatgtatct ccttgtttcg
    91801 taccttcaaa agttaccgtt ccggggaact gttgaaagct gatgaaaagg atggtatggg
    91861 caatacctta tatattggca gcctcaaaag cgaggtttat ttttgtttgt acgaaaaaga
    91921 ttatgaacaa tatatcaagt tagggattcc attggaccag accgaaacga aaaatcgttt
    91981 tgagattcgg ttgaaaaatg accgtgctta ttatgcgatt caagatttgt taaaaggtcg
    92041 tagcattgaa agtaccacgt tttccattat caatcggtat ttgcgatttg cggataaagt
    92101 agatggaaaa cggcgaacca attggccctt gaatgaacaa tggggtcgtt ttattggacg
    92161 gaatcggaaa gagattcaat taacttctga accgaaaccg tataccattg aacgaacatt
    92221 aaattggcta ggtagacaag tcgcccctac gtggaaaatg gctaaggagt tggatcgatt
    92281 gaatcaaaca acgtatatcc aggatatggt acagaatgcg cgactctcgg accggcataa
    92341 aaagattttg gagcaacaaa gtatggcaat cgaaaacttg attgtatgaa aagaggaatt
    92401 ttgatggaaa ttgtaaaaga tatgttattg gtacttggtg gcagtatgct tggtgttacc
    92461 gtgatgtgct tgatgaatgc aagtgcagca gcggattgag caatggaaaa gaaaggaaag
    92521 tgacaaaatg aatttcggac aaaatttata tcaatggttt ttaaccaatg ctcaatcttt
    92581 agtcttacta gcgattgtgg tcattggttt gtttttagga tttaaacggg agttttccaa
    92641 actgattggc ttcttggtga ttgctttgat tgcggtaggt ctggtcttta atgcttctgg
    92701 tgtgaaggat gtcctactca atttgtttaa ccgaattgtc ggtgcctgag ggggaatcac
    92761 aatggaacag atgcgtgttt atattgcgaa ccttggaaaa tacaatgaag gtgagttggt
    92821 gggggcatgg tttaccccac ctgtcgattt tgatgaagtg aaagaacaga ttggattaaa
    92881 tgacgaatat gaagagtacg cgattcatga ttatgaactg ccttttgaga ttgatgagta
    92941 tacgccaatt gaagagatta atcggttgtg taacttagcg gaggaactag aaggaacgcc
    93001 aattggtgaa gctgcttctg agattcaaca cgctttcttc aattctttcg aggaaatggt
    93061 agagcatgtg gatgatattg tctattatcc agattgcgat gatatgagtg atgtggcgca
    93121 ttacttgatt gtagaagctg gtgattttgg cgaagttcca gaacaattga agccgtatat
    93181 tgattttgat gcttgcggtc gagatttgga gattggagga aattatttgg ttactagtcg
    93241 tggagttttt gagtatccag aatgaatcaa acactatata tagtgtttga ttagtatatg
    93301 taagcaaaaa aacacaatat atagagtgca aactgaaggg taatgtgata taatatttgc
    93361 cgaagaaata tttttgaaat gaggtaatat tatggcaaat attcaaacaa gttttattga
    93421 tttccataat tcaattagat tggacgtaga agacaatacg ctacttaagg actataaaga
    93481 ccaagtaatt gatggattaa aagattactt acctgatgat gtgaagtttg aaactttttt
    93541 acagggaagc tattctgttt ataccggaat aaaaagctgt gatgaaaaaa ttgattttga
    93601 catcgatatt gcagttgctt tcgaaattga tcatactgtt tatgaagatc caagggagcc
    93661 taaactgtgg gtaaaagagg cgctagttga aatttttcct aatgctcaag ttaatttaaa
    93721 agtaccttgt gtaactgcta catttactgg aaaaaagaca aaaaaaaatg tgcacgttga
    93781 tgttgcggtt tatgctaaag aagatgaaaa ttatttctta gcaaaagcaa aagaattcag
    93841 tgctcccgaa aaccgatgct gggaggaagc ggatcccaaa gtattaaaag agaaaattaa
    93901 tagtcatgta gcagattcag atgatcgaaa acaatttcga agatgtattc gctatttaaa
    93961 aagatggaaa gataacaatt ttaaccaaga atataaacca acaggaattg gacttacaat
    94021 taatgtgatg gatacgttcc tagttaataa gtccactgat tttttaacta gaaaagttca
    94081 gtataatgat atggaatgta tgaagcagat tgtttcttcg ctgaaagatt catttgtgta
    94141 tgagtacagt gaaacagatg gttggcatta tcgtctacat gcatatcttc cagtgaagcc
    94201 taacagtgac acctattcaa aaatgacagt gaatcagatg agtgatttta aaaacaaact
    94261 ttctaaatta tatgatgatt tgattttcgc tatcgatact gaagatgagt atgaagctac
    94321 aaaacgatta aataatcaat ttggggagga ttttttaatt atttcagaag aagaagttac
    94381 agaaaaaaac ttaagaaatg catttgttac agactatccg agtgcttagg ttggtgttaa
    94441 agattggatt atactgatat tttaaaagaa aagctaacag atgttgtgcg tttattcgat
    94501 ggtaattact atggggattt ttcatttgct acaattggaa agttgcgttt aaaaattgaa
    94561 ataccaaaac attttccatt ggtacttcct aggatttaca ttcataattt tgatcattta
    94621 ggactttacc taccacatgt agaaaaagat gggttggtat gtttttcttc agatataaat
    94681 tatttagtag atgaagaaaa ttcagaattg gttttattgc agtgtgttaa taaaactata
    94741 aaaacaattg aggaaggttt gaaagggtta aataaaaatg attttcgaaa agagtttgtt
    94801 gctttttggg acaaac
//
LOCUS       DACDIH010000003        88091 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDIH010000003 DACDIH010000000
VERSION     DACDIH010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 88091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.003"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            169..1227
                     /locus_tag="IV281_000175"
     CDS             169..1227
                     /locus_tag="IV281_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP8779222.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            1246..2265
                     /locus_tag="IV281_000176"
     CDS             1246..2265
                     /locus_tag="IV281_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP8779223.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(2367..3083)
                     /locus_tag="IV281_000177"
     CDS             complement(2367..3083)
                     /locus_tag="IV281_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8779224.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(3052..3838)
                     /locus_tag="IV281_000178"
                     /pseudo
     CDS             complement(3052..3838)
                     /locus_tag="IV281_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
     gene            complement(3829..4578)
                     /locus_tag="IV281_000179"
     CDS             complement(3829..4578)
                     /locus_tag="IV281_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP8779225.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4876..5400
                     /locus_tag="IV281_000180"
     CDS             4876..5400
                     /locus_tag="IV281_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779226.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5416..6915
                     /locus_tag="IV281_000181"
     CDS             5416..6915
                     /locus_tag="IV281_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP8779227.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(7003..7605)
                     /locus_tag="IV281_000182"
     CDS             complement(7003..7605)
                     /locus_tag="IV281_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8779228.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7709..8665
                     /locus_tag="IV281_000183"
     CDS             7709..8665
                     /locus_tag="IV281_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP8779229.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(8970..9926)
                     /locus_tag="IV281_000184"
     CDS             complement(8970..9926)
                     /locus_tag="IV281_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8779230.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            10100..10660
                     /locus_tag="IV281_000185"
     CDS             10100..10660
                     /locus_tag="IV281_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779231.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10653..11477
                     /locus_tag="IV281_000186"
     CDS             10653..11477
                     /locus_tag="IV281_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP8779232.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11487..12104
                     /locus_tag="IV281_000187"
     CDS             11487..12104
                     /locus_tag="IV281_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP8779233.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            12122..12805
                     /locus_tag="IV281_000188"
     CDS             12122..12805
                     /locus_tag="IV281_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP8779234.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13488..14771
                     /locus_tag="IV281_000189"
     CDS             13488..14771
                     /locus_tag="IV281_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8779235.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            14787..15674
                     /locus_tag="IV281_000190"
     CDS             14787..15674
                     /locus_tag="IV281_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8779236.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15691..16527
                     /locus_tag="IV281_000191"
     CDS             15691..16527
                     /locus_tag="IV281_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8779237.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16530..17153
                     /locus_tag="IV281_000192"
     CDS             16530..17153
                     /locus_tag="IV281_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779238.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            17150..18442
                     /locus_tag="IV281_000193"
     CDS             17150..18442
                     /locus_tag="IV281_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8779239.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18801..18898
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            19014..19595
                     /locus_tag="IV281_000194"
     CDS             19014..19595
                     /locus_tag="IV281_000194"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP8779240.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19598..20905
                     /locus_tag="IV281_000195"
     CDS             19598..20905
                     /locus_tag="IV281_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP8779241.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            21321..21809
                     /gene="purE"
                     /locus_tag="IV281_000196"
     CDS             21321..21809
                     /gene="purE"
                     /locus_tag="IV281_000196"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP8779242.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21793..22926
                     /gene="purK"
                     /locus_tag="IV281_000197"
     CDS             21793..22926
                     /gene="purK"
                     /locus_tag="IV281_000197"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP8779243.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22939..24234
                     /gene="purB"
                     /locus_tag="IV281_000198"
     CDS             22939..24234
                     /gene="purB"
                     /locus_tag="IV281_000198"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP8779244.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24414..24791)
                     /locus_tag="IV281_000199"
     CDS             complement(24414..24791)
                     /locus_tag="IV281_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779245.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            25047..25775
                     /locus_tag="IV281_000200"
     CDS             25047..25775
                     /locus_tag="IV281_000200"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP8779246.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            25775..26029
                     /gene="purS"
                     /locus_tag="IV281_000201"
     CDS             25775..26029
                     /gene="purS"
                     /locus_tag="IV281_000201"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP8779247.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            26031..26702
                     /gene="purQ"
                     /locus_tag="IV281_000202"
     CDS             26031..26702
                     /gene="purQ"
                     /locus_tag="IV281_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP8779248.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            26703..28925
                     /gene="purL"
                     /locus_tag="IV281_000203"
     CDS             26703..28925
                     /gene="purL"
                     /locus_tag="IV281_000203"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP8779249.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            28910..30349
                     /locus_tag="IV281_000204"
     CDS             28910..30349
                     /locus_tag="IV281_000204"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP8779250.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            30381..31424
                     /locus_tag="IV281_000205"
     CDS             30381..31424
                     /locus_tag="IV281_000205"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP8779251.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            31421..31999
                     /gene="purN"
                     /locus_tag="IV281_000206"
     CDS             31421..31999
                     /gene="purN"
                     /locus_tag="IV281_000206"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP8779252.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            31996..33531
                     /gene="purH"
                     /locus_tag="IV281_000207"
     CDS             31996..33531
                     /gene="purH"
                     /locus_tag="IV281_000207"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP8779253.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            33547..34821
                     /gene="purD"
                     /locus_tag="IV281_000208"
     CDS             33547..34821
                     /gene="purD"
                     /locus_tag="IV281_000208"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP8779254.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            34943..35857
                     /locus_tag="IV281_000209"
     CDS             34943..35857
                     /locus_tag="IV281_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP8779255.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(35990..37555)
                     /locus_tag="IV281_000210"
     CDS             complement(35990..37555)
                     /locus_tag="IV281_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8779256.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(37548..38999)
                     /locus_tag="IV281_000211"
     CDS             complement(37548..38999)
                     /locus_tag="IV281_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8779257.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(39013..40029)
                     /locus_tag="IV281_000212"
     CDS             complement(39013..40029)
                     /locus_tag="IV281_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8779258.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            40249..41337
                     /locus_tag="IV281_000213"
     CDS             40249..41337
                     /locus_tag="IV281_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8779259.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            41391..42917
                     /locus_tag="IV281_000214"
     CDS             41391..42917
                     /locus_tag="IV281_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP8779260.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            42919..44124
                     /locus_tag="IV281_000215"
     CDS             42919..44124
                     /locus_tag="IV281_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8779261.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            44381..45358
                     /gene="guaC"
                     /locus_tag="IV281_000216"
     CDS             44381..45358
                     /gene="guaC"
                     /locus_tag="IV281_000216"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP8779262.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            45622..47046
                     /locus_tag="IV281_000217"
     CDS             45622..47046
                     /locus_tag="IV281_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8779263.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            47507..48616
                     /gene="pdhA"
                     /locus_tag="IV281_000218"
     CDS             47507..48616
                     /gene="pdhA"
                     /locus_tag="IV281_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP8779264.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            48619..49596
                     /locus_tag="IV281_000219"
     CDS             48619..49596
                     /locus_tag="IV281_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP8779265.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            49621..51264
                     /locus_tag="IV281_000220"
     CDS             49621..51264
                     /locus_tag="IV281_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP8779266.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            51272..52678
                     /gene="lpdA"
                     /locus_tag="IV281_000221"
     CDS             51272..52678
                     /gene="lpdA"
                     /locus_tag="IV281_000221"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP8779267.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            52840..53115
                     /locus_tag="IV281_000222"
     CDS             52840..53115
                     /locus_tag="IV281_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP8779268.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            53121..53888
                     /locus_tag="IV281_000223"
     CDS             53121..53888
                     /locus_tag="IV281_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP8779269.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            54056..55885
                     /gene="typA"
                     /locus_tag="IV281_000224"
     CDS             54056..55885
                     /gene="typA"
                     /locus_tag="IV281_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP8779270.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            56146..57918
                     /gene="recQ"
                     /locus_tag="IV281_000225"
     CDS             56146..57918
                     /gene="recQ"
                     /locus_tag="IV281_000225"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP8779271.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLRKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            58032..58334
                     /locus_tag="IV281_000226"
     CDS             58032..58334
                     /locus_tag="IV281_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP8779272.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            58337..59500
                     /locus_tag="IV281_000227"
     CDS             58337..59500
                     /locus_tag="IV281_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8779273.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            59526..62954
                     /locus_tag="IV281_000228"
     CDS             59526..62954
                     /locus_tag="IV281_000228"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP8779274.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            62975..64099
                     /locus_tag="IV281_000229"
     CDS             62975..64099
                     /locus_tag="IV281_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779275.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            64089..64526
                     /locus_tag="IV281_000230"
     CDS             64089..64526
                     /locus_tag="IV281_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8779276.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            64510..64848
                     /locus_tag="IV281_000231"
     CDS             64510..64848
                     /locus_tag="IV281_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP8779277.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            64967..65527
                     /gene="rsmD"
                     /locus_tag="IV281_000232"
     CDS             64967..65527
                     /gene="rsmD"
                     /locus_tag="IV281_000232"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP8779278.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            65524..66015
                     /gene="coaD"
                     /locus_tag="IV281_000233"
     CDS             65524..66015
                     /gene="coaD"
                     /locus_tag="IV281_000233"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP8779279.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            66005..67048
                     /locus_tag="IV281_000234"
     CDS             66005..67048
                     /locus_tag="IV281_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8779280.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            67207..67935
                     /locus_tag="IV281_000235"
     CDS             67207..67935
                     /locus_tag="IV281_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP8779281.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            68051..68296
                     /locus_tag="IV281_000236"
     CDS             68051..68296
                     /locus_tag="IV281_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8779282.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            68293..68511
                     /locus_tag="IV281_000237"
     CDS             68293..68511
                     /locus_tag="IV281_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP8779283.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            68647..69186
                     /locus_tag="IV281_000238"
     CDS             68647..69186
                     /locus_tag="IV281_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP8779284.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            69179..71452
                     /locus_tag="IV281_000239"
     CDS             69179..71452
                     /locus_tag="IV281_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP8779285.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            71490..72536
                     /gene="holA"
                     /locus_tag="IV281_000240"
     CDS             71490..72536
                     /gene="holA"
                     /locus_tag="IV281_000240"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP8779286.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(72625..72879)
                     /locus_tag="IV281_000241"
     CDS             complement(72625..72879)
                     /locus_tag="IV281_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP8779287.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(73279..73548)
                     /locus_tag="IV281_000242"
     CDS             complement(73279..73548)
                     /locus_tag="IV281_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779288.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(74018..74473)
                     /locus_tag="IV281_000243"
     CDS             complement(74018..74473)
                     /locus_tag="IV281_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP8779289.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(74611..76539)
                     /locus_tag="IV281_000244"
     CDS             complement(74611..76539)
                     /locus_tag="IV281_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8779290.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(76736..77569)
                     /locus_tag="IV281_000245"
     CDS             complement(76736..77569)
                     /locus_tag="IV281_000245"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP8779291.1"
                     /translation="MFFANIIKAVILGIIEGITEWVPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(77761..78465)
                     /locus_tag="IV281_000246"
     CDS             complement(77761..78465)
                     /locus_tag="IV281_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8779292.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(78488..79114)
                     /locus_tag="IV281_000247"
     CDS             complement(78488..79114)
                     /locus_tag="IV281_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8779293.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            79440..81815
                     /locus_tag="IV281_000248"
     CDS             79440..81815
                     /locus_tag="IV281_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP8779294.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(81839..82330)
                     /locus_tag="IV281_000249"
     CDS             complement(81839..82330)
                     /locus_tag="IV281_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP8779295.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            82517..83437
                     /locus_tag="IV281_000250"
     CDS             82517..83437
                     /locus_tag="IV281_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8779296.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            83591..83935
                     /locus_tag="IV281_000251"
     CDS             83591..83935
                     /locus_tag="IV281_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP8779297.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(84125..84625)
                     /locus_tag="IV281_000252"
     CDS             complement(84125..84625)
                     /locus_tag="IV281_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8779298.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            85153..85236
                     /locus_tag="IV281_000253"
     tRNA            85153..85236
                     /locus_tag="IV281_000253"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:85187..85189,aa:Leu,seq:cag)
     gene            complement(85560..86198)
                     /locus_tag="IV281_000254"
     CDS             complement(85560..86198)
                     /locus_tag="IV281_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8779299.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            86533..86967
                     /locus_tag="IV281_000255"
     CDS             86533..86967
                     /locus_tag="IV281_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8779300.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            86990..87310
                     /locus_tag="IV281_000256"
     CDS             86990..87310
                     /locus_tag="IV281_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779301.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            87451..87642
                     /locus_tag="IV281_000257"
     CDS             87451..87642
                     /locus_tag="IV281_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779302.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(87713..87970)
                     /locus_tag="IV281_000258"
     CDS             complement(87713..87970)
                     /locus_tag="IV281_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP8779303.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
BASE COUNT        28674 a        15197 c        18869 g        25351 t
ORIGIN      
        1 ttttttgtgt tctagtaaaa atcactttac ataagtttca aaagtttctt taatttccta
       61 ttgttcaata agtaacaatg taaagataaa attaaaaatg ttaatacgta ttagcaaaat
      121 aaaagctttt tttattgtga ataaatgaac ataattagga ggagaaagat gaacttattt
      181 gacatttttc gatcgacttt gacagatatg tcgattgtca gtgcaatcac ttcaacagtt
      241 tttattatat tattagggtt tgtttgtaga aaaaagggga ttttttctga atctgttgga
      301 aaaattctat cgaaagtcgt cttaacagtg gctttgccag cactggcatt tcgagctttc
      361 atgcaagata tcaatacaga attactgatg caaggaatca atgtcttgat ttggggatta
      421 gttatttata ttattcttat tctagtttca aaaccttttt tcttaagcta taaaggggat
      481 aagcaaacaa cgttgcgtgt cctgacgatc tttggttcta cgaccttttt cggaacgcct
      541 atcgtcagtg cgatttatgg tcctgtcggc gtgatgtatt cctcaatttt caatattggc
      601 taccgaatct ttttgtattc ttatggatac ataaaaatga gtggcttgaa gatggaagct
      661 aaaaatatca aagcgatgtt cgtcaatcca atcattatcg ctacttttgc cggccttttc
      721 atttggctgt tccaagggta tctgccacaa gtgtcagtta caggtgcaga aggagagata
      781 agccaagtag cgtttttgcg aattgaccag actgcaccgt ggctatttca gccgatggat
      841 tatttagctg gtttaagttc accattggcg tggctagcaa tcggcgcaac attaggtgaa
      901 gtaagcttca aaaaagctgc atctgaaaaa acttcatggt attacagtct agtcaaagtg
      961 gtatttgtac ctgcattgaa tatcgtgatt ctagcagttt tgacggcaac gaatattcta
     1021 ccagtcaatt ttgaagcact ggcaacaatc gttatcatga tggctacacc agctgcaaca
     1081 gtggcagcag cctatgctat cagttttgat agggaggcac ttttagcctc caatgcatcc
     1141 ttactgtcaa ctatttttgc tgtggtactg acacctgtat ggatcgttgt attacaaatg
     1201 attcatggca taggtttatt ttcataagaa atggaggaag aaagtatgag aatcgcatgt
     1261 tacggtgttc gtccgaacga gatagatttt tttgaaaaat tgaatattta ccattatgat
     1321 ctcagtcttt atgaagaact gttgacacat gacaatatcg tgtcagcaaa ggaccatgat
     1381 gctgtattgt tgaggggaaa ttgtatagca gatgagacca atcttgcaaa aatgcaggaa
     1441 tatgggattc gttatgtttt tacacggact gtgggagtca atcatatcga tctacaagcg
     1501 gcagctgatt ttggtatgac agtagcccgt gtaccatcgt attcgccaaa tgcgatagca
     1561 gagttatctt tgacttttgc catgatgctt ttgagaaata ctgcgtatac gacgatccga
     1621 acatctttca aagattttcg agtggatgag caaatgttta gtcgtgaaat cagaaattgc
     1681 acagtaggga tcataggaac aggcaggata gggttgacgg aagctgtttt gtttaaggga
     1741 ctaggagcca aagtgatcgg ctacgatatt catcagacag aagctgctaa aaaagtattg
     1801 acgttccact cgcttgaaga gttattagcc aaaagcgata ttgtcagtat tcatgtacct
     1861 catatcccag gtgaaaacga tcaaatgatc aatgaaggat ttttagctca aatgaaaaaa
     1921 ggtagcatct tgattaatac agcccgtgga gagcttcaag ataatgaagc tattttaaga
     1981 gctttgaaaa gcaatcattt agctggtttt gcaaccgatg tttttgcaaa tgaaacagag
     2041 gtttttttcc gatccttcaa gccatgggag acgattccag accctgctat ccagcaatta
     2101 gtagaaatgt accctagagt tcttgtaacc ccgcatgtag gttcaaatac agatgaggca
     2161 ttatcaaata tgatttcgac aagtttcgag aactttcgtg aaataataga aactggaaaa
     2221 acgaaaaatg aggtatctct tcctaaagcc cgtcaattaa gataagaaaa catggatatt
     2281 cgtcaataaa atgtttaaaa atagtccaag taaacagttg ttacttctgt atacttggac
     2341 tattttgtat gggataattc gatgactcaa cagtaatctg tgagcttttt ccgcccattc
     2401 tttgtcaatt cataacaggt tagcggccgg ccgatttcca aatatttgat tctttcgttc
     2461 aatatatctt gttctactaa aaaattcaaa tatttcttga cggatatacg tgacagtgag
     2521 cttttttgag ccagttcttt tgttgaaaaa atagtatctt cttttagaat aaatgcactt
     2581 atttttttta aagtcagttt tgacagacct ttaggcaaat cgatgaatga tggtatttcg
     2641 tgaatgggag tggtgtggtt gaatacttga tcgatcaatt tctgatcggc tttttcagtc
     2701 gtttggattg tctgagagaa agcaagatac ttttcgatcg ccagctgaaa acgttgcaaa
     2761 gtgaacggtt tgatcaaata atctaaggca ccgtagcgga tcacattctt tatggtttcg
     2821 atatcatctg cagcagtgat caaaatcact gggatatcat ttccttgctg tctaagccaa
     2881 gataaaaaat ggataccact ttcgccaggc atgtatacat caagcagcag cagatctgcg
     2941 tttttttgtc tgaaatattt ttttccttct tctacatttc tgaaactttc gattacgttc
     3001 atattaggga tgctttccac aaaagtacga ttcaattctg caaccattgg atcatcttcg
     3061 attatcagga catttgtcat cattttgatc ttcctttttt ggtaattcta gatagacgat
     3121 tgtaccgcca tttgtttgtt tggaaaattc taaaaatcca ccgtggcgtt tgacgattga
     3181 atggatgaga ttcaatccaa acccacgatt gattcccttc gtcgaaaaac ctagcgtaaa
     3241 tattttttct gagatttctt ctgatatccc gacaccgtca tcgcagacgg tcacgatgaa
     3301 tacttcagct tctgtgtcat agtttaaatg aatccggacc tgtccgtttc tttcatgttt
     3361 ttgtagcacg gcttcttttg cattatcaag taagttccct aacacttgca aaagtgaatg
     3421 gatggagtca tccatctcca ttttagggat aaaagaatcg ctttctaaca gcatttcaac
     3481 agattgttct ttggcttcgt ttattttgcc taataaaaaa ccggcaattg caggtacttt
     3541 gatcagactt gagacttggc caatctcttt tttataattt tgatttagct gctggataaa
     3601 atgggtgact tcttcatact tcttcaaatc aatcaaacca gaaatgacat gaagtttatt
     3661 catgaattca tgggtttgag ctctcatgga gtttatatat tgtttcgtcc ccccaagctc
     3721 ctccattaat ttcattactt ctgatcgatt tcgtaaagtt gcgactgctc ctaaaaaaac
     3781 ggtcattgct aaaaagaggt gtaacgctag aaatagtttc tatcccattt aaatacatgg
     3841 attgagtccg aacggtcgtt tgatgctcaa tcgtctcttt aaacaaaacg tcatatagat
     3901 tatcactgat ttttttccta cttcgaaatc caatagctgc tctgtttggt agaaaatgtt
     3961 ttttgcaggt gtattgatca agaggatagt tttatcagaa gagatggcta agattccttc
     4021 atctacttca ttttcgataa tcgttttttc acttaactgt tctgctattt cttgtggttc
     4081 aagtccaagc agtacttttt tcattttttg ggcaagaaaa acggcgccta tgatgcctac
     4141 gctaagtcca ataatcaatg aaaaaacaat cattttttgt ccatctttga cttgattgtc
     4201 aatcgtgttg tttgttaagc caacacaaac tgcaccaata atctcttgat cataataaat
     4261 agggacgaaa tagcggaagc cgaaccctaa cattccttct ttttcagaga aaaattcttg
     4321 tcctccaatc acttttttca catcttcatg acttgagaaa ggaagaccga tgtaacttgg
     4381 gtctgcgtga gaatatcgaa tgccttcagg gtccatgaca acgataaaat ctaaattcga
     4441 tgcaatcatg atttcatttg ttattccttg aatcgcatgt gtcgggtcac cagtctgcag
     4501 ctttttttgg atgagtttat cattggcaac gattttagca atgctagaga ttttatctga
     4561 cacactagat cggttcatcg aaagaataaa attcctgatc aatatggcag aaacaatcaa
     4621 agaaacgaat gtggagaaac aaacgacaag gatgatcgtg ttctggagct ttaatggttt
     4681 tttcttaaaa aaaggaaaca taggctacct cgaaagaatt tattgtgaaa attttaacat
     4741 gaaaacaaaa atttaacaaa ttgtcaacga taaacacaac cttcctataa aaaacaccac
     4801 tgagtcggta aaagaagcta tacagaattt tcaaacattg gtatgataga cgtagaaatg
     4861 gaaggaggcg tgtatatgat agggggaatc gcacttgaga caatttattg gtatacattg
     4921 ctgatttgtg caggattggc agtcctattg attatttttg gagatgtatt cgattttgat
     4981 ggcccgcttg atccgatgct tattatccct tggttaactt tcacaagctt atttggtttt
     5041 ctaggtgagc gtttaacaga tcaatcatct ttatttttat ttattgctag tggaggcata
     5101 tcgatagtgt tggtattttt gcttaatttc tatatattga tgccgatgaa acatgccgaa
     5161 tcaacgatgt cgtcttctga aaaaactctg gaaggacaag tagctgttgt gatcacaccg
     5221 atccctgtac gaggtatggg agaaattcag ttgaaaagtg ttaccggttc cattagtcgt
     5281 ccagcttgtt tttacagccc ccaagaacag acagctgagc ggggaacgaa agtattgatc
     5341 attgagatca aggaaagaat ctgttatgtc atgccttatg aaagcatgat aaaagcttaa
     5401 aggagcgtgt gttaaatgga aataccagtt gtttttatcg cgatcgcaat cgcagctttg
     5461 attttactga tgttgctgat cgtctttgta gcgaagtacc agacagcaaa accggatgaa
     5521 gcgttgatca tcagtggaag ttatttagga agcaaaaatg tgcacattga tgaaagcaat
     5581 aacaaaatca aaattgtgcg tggaggtggc gcattcgtct tgccagtttt tcaacgttcg
     5641 aatcggatca gtctattatc gagtaaattg gacgtctcca cccctgaagt gtatacggaa
     5701 caaggcgtac cggtgatgtg tgatggtact tcgatcatca aaatcggttc atctgtagaa
     5761 gagattgcta cagcagcaga acagttttta ggcaagacaa gagaagaact ggaaaatgaa
     5821 gcaagagaag tattagaagg acacttgcgc tcgattcttg gttctatgac tgtagaagaa
     5881 atctatcaaa atcgcgataa gttcagtcaa agcgtacaag aagtcgcttc tgtagacctt
     5941 gcaaaaatgg ggttgatcat cgtttctttc actatcaaag aggtcagaga caaaaacggt
     6001 tatcttgact ctttagggaa accaagaatc gcccaagtca aaagagatgc agatatagca
     6061 gaagcggaag ctctaaaaga aacgcgtatc aaaaaagctc aagcagaaaa agaatcacaa
     6121 gcagctgaat tacagcgaca aacagagatc gcagaatcat taaaagaaaa agaactgaaa
     6181 ttagcaacct acaaacaaga acaagatgtt gctaaagcaa aagcagacca agcctacaat
     6241 ttagaaagtg cccgagcaca acaacaagta atcgaacagg aaatgcagat taaaatcgta
     6301 gaacgtcaaa aacaaatcga actggaagaa aaagaaatca cacgtcgtga aaagcaatat
     6361 gattcggaag tgaagaaaaa agcagatgcc gatcggtacg ctaaagaaca agaagcacaa
     6421 gctcaaaaag tcaaagaagt gactgaagcc gaagcagaac gattccgtgt ggaagcactg
     6481 gcagaagcgg aagccaataa gacacgttta gctggacaag cagaagccga agcagcacta
     6541 gcaaaaggaa aagcagaagc cgaagcaaaa caaaagatcg ccaatgcctt caaggaatat
     6601 ggcgaagcag ctgtcttgag tatggttatc gatatgctac cgcaattgat gcgcgaagca
     6661 gcacagccgc taggaaatat cgaaaaaatc tctgttgtcg atacaggaag cagttcagga
     6721 gaaaccagtg gagcgaaccg agtgacaaat tatgccacaa atttgctttc ttctactcaa
     6781 gaaacactaa aagcgacaac aggacttgac ttgaaggaaa tcgtagagaa tttgtcgaca
     6841 cgtgggacta ctagccaagt aaatatgtat gaagcgccaa aagaggcaga acaaaactcg
     6901 atgaaggaag aataaagtag tacacttcac cgttcaatca aaaatggagg aagacttttt
     6961 aaagttcttc tctccatttt tttattttat tgcaaaagaa aattagtcct cgctattttt
     7021 cttttgtttt tggatcttag cttctatctc ttgtaaattt gttcttagca atcgttcttt
     7081 cttttggagt tcgaccaaat cttctgatag ttcttccttc ttttctgttt caggaggacg
     7141 aagaaagaaa ttgataccga ttgcaatgaa tgtccctata aaagtatcta gtacacgttg
     7201 gatagcatac aaagcggatt cgccttgagg gatgctcaac gcaatcagca acaacgtagc
     7261 tattgcggaa atgataccgg aattattgtt aataccatct gacacgacga tcacaataat
     7321 aactagaata ggaagcaaga tcaattctac caaaaaatca ttgtgaaaat agcttttgac
     7381 aaaaaaataa atgatagcta atgtaccacc taatgtattg ccgatgatcc tagactttcc
     7441 aaaagaaacg gttgtagtca agtcctgacg caaagaaaaa acagcagata atgcagcaat
     7501 catcgggatt cctcgttgag tcaaatgaaa aaacaagata cataaaaaaa cagctaaggc
     7561 acttttgatc gtacgcatac ctagtcgaaa acggccaaat tgcatagaga ttctcctttt
     7621 tggtcttttt actatcatac ctaaactttt gtctgatagc tagtttttca ttattcaagt
     7681 ataattaaaa taaaaaaggg ggaagtaaat gcgtatagga atgcgaacag tcaaaacagt
     7741 gatcagtgtc ttcttttcga tggtcacagc gggtagctta tcgctgttgt attggccagc
     7801 tgcagggatc attgcattat taagtgtagg aaataccaag cgctctacat tgcgtacggg
     7861 catgaatcga atcgcagcct ttatattagc tacggcaaca gcactgttca gtttttttgc
     7921 tgttgggtat actatttttg gcttcagtct ttttttattc ctgttcatcc cgcttgcctc
     7981 tcgcttcaaa ttaaccgaag ggatagtagt aaattcggtt ttggtcactc attatttagt
     8041 ggaggaaaac atgtcttggc agctgatcgg caatgaagcg ggattaatgg cgattggatt
     8101 agtatttgct ttgcttgcaa atgtttacat gccagatcgt acgaagcagc tgaaggaaaa
     8161 ccaaatacag atcgaaaaag aatttcgaaa tattttgaga caaatggcag aatttctttt
     8221 atcagaaaat aaagaagatg tgcagatcaa atgcgaacat ttactgacat tcattcgcga
     8281 aagccaagaa gatgccagag aacatcaaga aaactactgg ctcagacagc cgctatacta
     8341 cgaaacttac ttctctatga gaagagcaca ggccaatgtg attaaagaca tgctggaaaa
     8401 tctagagaga atccagcaac cagcttatta tggaaaacat atctatggtt tattgattta
     8461 tactgcagaa acattttcag agtcaaatga tggcagacaa attttaacac gtattgaaga
     8521 agtttatgta ttgtatcggc aaatgcctct gccgactagt cgccctgaat tcgaggatcg
     8581 agcagaattg ttccagtttt tgcagtcttt taaaagtttt attgaaatca aggcagaatt
     8641 ctcccaacaa aaaatccaaa aatagaggtt gtgaattcag ctaatattca ggtcaaaaag
     8701 ctgatttcac aacctcttct ttaaacggtg ttctcctgtc aactcctcgg tcacaagtca
     8761 caatattcaa caaacatgag aagctgtttt ttgaaattgc tccttgtgac tcggagctga
     8821 acggtaggct cacaacctct ttcttaaacg gtgttcaaaa gctttcctct gcggtaataa
     8881 gtccagccaa acgaaaaata ggaggagcta tttttgtttg gctgttctta ttacttgatg
     8941 cagaacagct ttttcacaac cttttaaaat tacatatttt tccatagaac ttgcatttga
     9001 tccaagattt cgatgacatc ttttgtcaag aagagagagc gattgggcaa gtcaccttca
     9061 atcatgtgta ccatattctt tatttcgtaa ttcattgctt cagatgtatt cccgctttcg
     9121 atgaattcct ttgtaccatc tgtaaataag atctccgcac gatcagcacg cgggtaatca
     9181 gcaatcgtca cataaccgtt ctcaaaggca acgataccga cttttggcat ttttgcctga
     9241 aaagtcaagc tgacagcagc aagttcattt tctttgttac gcaagatagt gacggattgt
     9301 tcgtccacgc ctgtttcaaa aggaaccatt gtagaggcaa ctacttcagg ttgagagctc
     9361 aaaaagaaac gagcgaaaga tacagcatac gtaccgatat ccaacagagc tcctccagct
     9421 aattctggat tgaagaaccg gtttttaggg tctggttctt tgtaactgcc gaaaggtgct
     9481 tggatcattt ttaaagcccc cagttttcct gtatccatta ttgagcgtaa ctgctgataa
     9541 agtggcatgt tgaagattgt catcgcttct gctaagatca aattcttttc ttctgctaag
     9601 cgcatagcgt cagccagttc ttttttattc atcgtgatgg ctttttcaca tagaacgtgt
     9661 ttgcctgctt ccaatgctgc caagatatgg tcgatatgct gtctatttgg tacagcaatg
     9721 tacacgatgt cgatttcagc atcgtctaac atttcttgat agcttccgta agcttttgga
     9781 atattgtaac ggtgagcgaa gttttccgct ttttctgaag ttctggaagc aactgctgtt
     9841 agttcgctcg tctcttgatt gaagctttcg gcaaactcat gtgcgatggt accaagacca
     9901 acgattcccc attgatattt tttcatctga aaacgctcct tcatagttgg attgagtttc
     9961 ttgtctgtct attattataa caaaaatcac gaagaataaa gaggagagga cttgaaacat
    10021 taaaaaaaat gaaagcagcg atttaagagg tgctttttga tcaaaactta agtagaatag
    10081 ttaaaaggag gggataaacg tgcatcaagt atatattgat cttattatga gtgtattatt
    10141 agaacagttt gaaactgaaa aaaatttttt agatgaatac ttgctgatca gtccagaaca
    10201 atggcaaaaa tggaaagatg gcgagaaaaa tctgccatct gaagcgatgc agaaagtaaa
    10261 aaatcttttc actgattatg aatggatgct aacgcaaaag attttgcgtc aaacgattat
    10321 ttttcccgaa aaacgaaacg ttgccgttgc agagtacaag caaatcaaaa cacgcatcgc
    10381 gcaaaaatgg cttagcagca aagcagcaaa agtcgagctg atccctttga aaaataatca
    10441 gcaggctggt agataccttg acttgaaagt aagtattcaa tatgatgaat ggggctatga
    10501 tgatattttg aattttcgtc tgccagcaga tgttcaaaaa cagatcgaag gagaaaaaat
    10561 cgaacttctc gattgggtga atgaaaatct ggaagaaact tacgttcatg aggcgaatgg
    10621 ggaacgtgaa gaacaacgaa aggatttttc aagtgaataa atttttagga tttggagcag
    10681 ttgtgattat gatcgcagca atgggctatg ttatttttag cgatgatgat tcgcatgttc
    10741 aagccggcaa tcaagcagtc gaacgaacaa gcaccaaaac aactgctagt aaagaaaaga
    10801 acagtcaaac aaccagcagc aaggtaaagg acaaagaaac aaaagatcgt acgtccaaac
    10861 aattgccaga tgttaaaaca gacgattgga agctagtttt ggttggtcca gatcacaaat
    10921 tagaaaaaga aattgatgag tctactcaat tggcatcatt aagtaacgga tacttgatcg
    10981 acaaacgcat tgctccagaa tatgaagcat ttgccaaggc tgctgaagca gctggatttc
    11041 cattagtgat ggtctcagct taccggtctg tatcatccca acaacaagtt ttcgaacaaa
    11101 atgtccaaga tgtgatgagt cgtcaaggtg tatcagaaga ggaggcgaca aagattacta
    11161 aagaaacgat tacggagcca ggatacagcg aacaccacac aggtctggca gtcgatgtcg
    11221 tcgatcaaaa ctggtacaac agttatcctt ctactgtatt ggatgcaagt tacggagatc
    11281 agcctggagc taaatggatc gcagataatg ctgcgcagta tggatttatc gttcgttatc
    11341 caaaaggaca cgaagatata actaagatca catatgaacc ttggcactta cgttatgtcg
    11401 gtaaagaaaa cgctgagtat atcaccaaac accaattgac gctagaagaa tatctgaatg
    11461 aattaaatga aaagtaggaa ttgatcatgg cgaaaaaaaa gtggaaaaag caaaagcgca
    11521 agaatatccg ttggccagca gtggttgccg gtgtagcagt gatactctta gcctttgttt
    11581 tttccttgaa gaatctgtct tctcctttta ctgataatca gcaagaaaat gaagcgcaat
    11641 cccatcagca atttattgaa cggcttgttc cgcatgccca agaactacaa gatggttatg
    11701 gagtgcttcc tagtatcatt ttaggacaag ctatattaga atcaaattgg ggaaaaagta
    11761 ccttggctag caaatacaat aatcttttcg ggatcaaggc ttatggggat cagaagaaag
    11821 taagcctgga aacaaaggaa tttgttaacg aagaatggat aactatccaa ggagacttca
    11881 aagtgtatga ttcatgggaa cagtccatgg atgaccatac acaactgttc gtccaaggag
    11941 tagattggaa tcctgcgctg tatgaaaaag tgattacggc caccaactat caggaagccg
    12001 cacaagcatt gcaagatgca ggttatgcga cagaccccgg ttatgcgcaa aaaatcatcc
    12061 aggtcatcga gacgtatcaa ttgaatcagt atgaccggag atagcaaagg agaagtggct
    12121 gatggcagaa caatttacgc cagaaatcat gacggaattg agaaaagata tcgtaagagt
    12181 accggaaatc attttagaag caagcgggat caagatattt ggaaaattga tccgttcgat
    12241 catctttact acggatattg cgatcatacg taatacaaat gcgaatgcag taattgctgt
    12301 gtatcctttt acacctcatc cagcgattac taaaagcatt attgaggcag ctgacattcc
    12361 agttttctcc ggtgtaggtg gtggtttgac acaaggtgct cgttcttcct acatgagcat
    12421 gttcgcagaa gcgcaaggtt cgttaggggt cgttttgaac ggtcctacgc ctttagagac
    12481 agtaaaagaa gtttgtcgag tagttgatat tccagttatc agtacggtaa cgtcgaaata
    12541 tacagaaata gatgaaaagt tagcagctgg tatttcgatt atcaatatca gtgcaggtaa
    12601 agaaactgca gaagcagtac gccattttcg taaaaaatat cctgaacttc ctatcattgc
    12661 gacaggtggt ccaacggaag aaagtattgc tgagaccatt caagcaggag caaatgccat
    12721 cacatatacg ccaccaagta gcggagagct ttttagtcgt aaaatggaaa aatatcggga
    12781 aactgagaaa aagaatcatg aataacaggt gagaatcaaa agacgaggaa gagcagatag
    12841 tggctcttcc tcgtcttttg tactccagta ctatttgttc agaattcaaa aaaaagagaa
    12901 atttttttgt ttttttacct gtaatgtaca taagaagagt taaaaccttt tgttattatt
    12961 agcattctaa caaaaaaatg tcttgattta ttgaagcgct ttctatagaa tgaaaaacga
    13021 ataaaaacta aaaaaggcac ccgacaattt agtagcagct aaattgtcgg ggcttgaaaa
    13081 gtcagcggga actgaccaat caagttgcca tagtcaatgc ttgtgcatcg acgtgtatat
    13141 tgtacctaat ttcatacaga attactagtt atttttctgt tttttaggga agagcgcaca
    13201 cgaagaagca tgcttattta gctatgtcaa ggatattggg cagttgtctg atatcctttt
    13261 ttctttgcgt aattatcctc ccatcattga agcaagaagt gtttttagcg atgaaagaag
    13321 aatcagcact atctaatttt tcttgaaagt attgagttgt tcatcctagg cagaacgata
    13381 aggcaagtct tggtttttat tccccttgaa aaagatcgtt taataaatat gttctgtata
    13441 aaaatacagt atattgaaaa ataacaatgg aagtagaggt gcagagaatg aagaaaagaa
    13501 tgagaatcat cttagcttta agtgttatgt tattcttctt gacggcttgt gggcaagaag
    13561 atgacaaaaa aatcacgctg aacatggctt ggtggggttc gcaagtccga cacgatgcaa
    13621 cagtaaaagt aatcgagctt tatgaagaaa aaaatcccca tgtgacgatc gattatgaat
    13681 tttatgatta tgaaggatat tacacaaaat taggtacact agcagcatca gatgatatct
    13741 gggacatttt ccaactgaac aatcaatttc ctcaatacat caatcagatt gaaccattag
    13801 atgaatatgt ggagcaaggg ataattgata tttctaatgc agatgaatcc tatttagcaa
    13861 cgacgactta cgacgggcaa ttagttggac tgtctaacgg ggtgaattcg tttgcgattg
    13921 cttacaataa aaatatattt gaacaattaa atatccctga accagcttca aattggacat
    13981 gggatgaatt tgaacaaatc agctatcaaa tcactgaaga attaggaatc tatgctattt
    14041 caagattcga agattttata gctggctgta ttattcaaat accacaagtt gagaaaggat
    14101 tgaattttta taatcgggaa gatctatcta acagcttggg atttgaaaat ccagactatc
    14161 taacagattt tttccagctg cgtaaaaatc tggtagacaa aggggcccat ccagaaccag
    14221 gtggtggagc tgcaagtacg acggatctgt cttttcaggc ggttagggat agagaagcgg
    14281 caatggtgtt tctatcaagt aaccagttga cagaaatatt gtcaggggct cctgaagata
    14341 tggagattca gttgattaat ccacctcgca gaaaagcaga tggagaatct ggtatccctt
    14401 tacgttcttc gcaaatgctg agtatggcaa aaagctcagc ccataaagaa gaagctgcta
    14461 agtttattga tttctttcaa aacagtgaag aagccaatga aattttaaaa ggagaacgtg
    14521 gtgtaccaat catgtcgaat attcgagaac agctcgctcg tgcagatgat gcaactttgc
    14581 aagcgacatt tgattattta gaaatgattt ccgatatgga caacggcgaa gtgaatgtct
    14641 ttgatagtgc tttgaatccg gaaattcaag accaatacaa tcttttagtt gaaaaagtca
    14701 tttatgaaga agtaacagca gaagaagcag cagagagtct ttatgagttt gcgtcaaaca
    14761 ttctaggata gcagcaggag gaaggcatga aaaaagaaaa actaaaacgg ttcttggatc
    14821 aagacaatgt ggtcggttac gtatttgctt ctccattcat cctaggattt ctattgataa
    14881 cagtgatacc gatgatcttg tcactttatt attcgttcac cgattatcgt ttgggaaatc
    14941 cagtctcatg gatcggcttg gataattata taaagatgct gagtgatgcc cgttttttga
    15001 actccatccg agtcactttt ctctatgtat ttgcatctgt acctgtgaaa ctgatctttg
    15061 gtctattgat tgcttatctt ttaacgagga atatcaaagg aacgactttc tttcgttctt
    15121 tgtattatgt gcctagtttg atcggtggca gtatttctat cgctctagtt tggcgggagt
    15181 tgttttctaa aaacgggtta gtgaatattt tcctggaacg attaggcgca cctgcgcttt
    15241 cctggtttag cgatcaaaaa ctggtcatga tccctcttgt tttgatgtct gcttggcaat
    15301 tcggttcggc aatgatcatc tttgctgcag gtatcaaaga gatcccaggc tcgtattatg
    15361 aagcggcaga gatcgatgga gcgaacaaat ggcagacgtt ttacaagatc acgttacctc
    15421 ttctttcgcc gatcattttg tataacttgg ttatgcagac aatcgcggcg tttatgactt
    15481 ttacgcaggc atttatcatt acccaaggtg gtccgaatga tgccacgaac ttttatgctc
    15541 tttatgtgta taaccaagct ttttctttta attctatggg gtatgctagt gctatttcgt
    15601 ggatcatgct gttgattatg ggagctgtca cctttctgat tttcaaatca tcaaaaaaat
    15661 gggtcgttgt gtgaatgaga ggaggaagaa atgaataata cagtaacgaa atggtttcaa
    15721 tacggtttta ttttgctatt aggtctagtc atgatttatc cggtcttatg gatgatcgca
    15781 ggttctttta aaacggatca agagattatg agtggttcgc tgaatttgat tccagagact
    15841 tttcaatggg aaaattacgc aaaaggatgg gcggggtttg ctggaatttc attttttact
    15901 ttctttagga attcgtttgt catctctgca gtttcgacga tagggacagt tctgagctcg
    15961 acttgtattg cttacgcatt atcgagaatc gatttcaaag gaaaaagatt ctggttcgtc
    16021 gtcatgttag taacgatgat gattcctgca caagtgattt tgattccgca gtttattatt
    16081 tacaatcggc tgaatttggt tgggacgtat gtgccgctga ttttaccaca tttttttgga
    16141 caagcatttt tcatttacca gatcatgcaa ttcatggtca gcatccccaa agagttagat
    16201 gaatcagcga ttattgacgg atgcagcaag tatagtgtct ttaccaaaat tatttttcct
    16261 ttgctgaaac cttcgatcat cacgacgatc atcatccagt tttattggaa atgggacgac
    16321 ttcatgggac cattgattta tttaaacaag ccgagatctt acacggtatc gattgccatc
    16381 aaattatttg cagacgctgg atctagtacg acttacgcga gtatgtttgc gatgtctacg
    16441 ttgtcgctgc taccggtttt tctgattttc ttattcttca ataaatatct tgtccagggg
    16501 atcagtacaa gcggtctgaa gggctgaata tgaaaaagcc aatagaagag atgtttcttt
    16561 acaagctcaa ctactatgtc tgtcggattt ttttaaccag tcttatcagt aatgctcttt
    16621 tcgctttagt tctaggatgt ttgttcactt taggattagt gctgtctgct cgtgttgtct
    16681 atctgattta ttttttgatg atgtatcctt cttattatct atctgtccat tacttggcag
    16741 aaaaaaaggt atgttggagg aaaggaaaaa tcaaacggat gtgtttcatc attacgttgt
    16801 ttcagctgtt tgtcattatt tttgcagttg attggtattt attagtggga aagattggag
    16861 cttggtatct gattcctttt ctattgtgtc tgttgctttt tgtatcccat agttttttta
    16921 gtacgctaat tgatatctgc aaagaagata aagcagatag tcttgtttat ttgatcaaaa
    16981 ggaatatctg gctgttgtca aaaaacgtca aggggtttct gtggtattta tgccttttgt
    17041 atggactgtt ctggtgtata cagcatgatt ttttactcac tgcattactg tatccagggc
    17101 tgccatttgt cacagtattt tgcttaaaag aaaaggagaa tgactttaaa tgaagaaaaa
    17161 atatgtacaa gtcggacttg gtgggagagc gcgttttttt tatgaggcaa ttgcctctac
    17221 gtatcatcaa acttgtgaac tagtcggttt ttgtgatgta tctcaaacaa ggatggatta
    17281 tgcaaatcgg atacttgtga acaagtatgg acagaaaaaa gtaaagacat gtatgccatc
    17341 acaatttgag caactactgg atgacgtacg gccggctgaa gtcattatta cgtcagtaga
    17401 tcgaacgcat catgattata tcattcgtgc aatgaaaaaa ggatatgatg ttatttgcga
    17461 aaaacctctg actatcgatg aggaaaaagc tcaagaaata ataacggtcc aaaaacagac
    17521 gggaagaagc ctgcgagtca cttttaatta tcgttatgca ccgtatcaca cgaagataaa
    17581 ggaactgata gaaagtgatg tgattggtga tgtgttttct atccacttcg aatggctgtt
    17641 aaatacgcaa cacggggcag attatttcag aagatggcat cgaaataagc acaatagcgg
    17701 cgggcttctt gttcataaat ctacccatca ttttgatcta gtgaattttt ggttgaacag
    17761 ccgtccccaa aaggtatttg cattcggtgg attaaacttt tatgggatgc ataatgcgga
    17821 gaaacgcgga gtagaaatat tttacagtcg atgctatgga agcgaactag ctaaagctga
    17881 tccatttgct atagacctgt cagccaatga ggaattgcga gaaatgtatt tagaagcaga
    17941 aaaagacagt ggatatattc gtgaccgcag tgtattttcc gatgatatct ctattgaaga
    18001 tacactaggc gtaacggtca aatatgaaac tggtgcgatt ttgacgtatt cgctaaacgc
    18061 gtatatgccg tgggaaggat tcaatgtttc tttcaatggc agcaaaggac ggatcgaaat
    18121 gaaagtgatc gaaaagtctt acatcaatgc aggtggagat aaaaaggatg aaggagcagc
    18181 cgttcttcag tccatccatg tctatccgat gttcggtgag ccatatgagg taatcgtcga
    18241 acaagaagcg ggcggacatg gcggaggaga tccgcgttta ttagatgatc tatttggtga
    18301 agaaaaagaa gaagatccgt ggaatcgggc agcaactcac gtagatggta ttttgtcgat
    18361 tttgacagga atcgcagcaa atcattctat tgcttcagga aaagcagtag tgattgacga
    18421 gcttgtcagc ttaaaagaat aagaatagat ttacggaaat tacttcccgt agtttataaa
    18481 atcttgcctt attgccgata ggctgtcttt tgaacactat ttattcggaa aaaggggcta
    18541 tgacatgctt tttgtcatgc cttcttttta tgtgggctgc ttttttagat aggagaaaat
    18601 aaaaataact atttaaaccc gaacaaaaag gaataatgca ttaaaaatca ttagatttta
    18661 caaaaaaata agtaaaccac gttgtcagtt tcagaaagta gtgttagact gttttcaata
    18721 tttgaaacaa cattttttat tcaaatatgg caaactgcct tcacgtgcaa aacagttgca
    18781 agaaaatgaa tttacactat tattagtttt acatacctac ttatatatcg tcataatatg
    18841 gatgacagtt tctagccagt accgtaaatg ctggactata agtaaaagat tggctatttc
    18901 acccttcttt tcttatgata aagtggaggg tgtttttctt taacagaaac atagtaggtt
    18961 tttgcacaaa agcggttatt tcggtattaa taaatgataa aggagcaaat aaagtgaaag
    19021 aattagttga acgtattcaa aaagacggac gtatattagg cgagggcgta ttaaaagtcg
    19081 acagcttcgt cactcaccaa gtagaccctg tattgatgga acaaatggga aaacggtttg
    19141 cagaagtatt taaagaacaa ggaatcacga aagttgtgac cattgaagct tctgggatcg
    19201 caccagcttt atttgctgca agagaattag atgttccaat gatcttcgct agaaaagcaa
    19261 aaagtttgac gatggatgaa gaattattga cagcttctgt ttattctttt acgaaacaag
    19321 tgacaagtac gatttctatt tctcgtaaat ttttatcttc agaagacaaa gtattgatta
    19381 tcgacgattt cttggcaaac ggacaagcgg caaaaggatt gatcgaatta tgccagcaag
    19441 ctggagcgca agtagaagga atcgggatcg tcatcgaaaa gtctttccaa gatggacgtc
    19501 agttacttga agaaatggga ttgagagtcg tttctcttgc gcgtattgct tccttgacga
    19561 atggacaagt tgagtttctt gaggaggacg cataatcgtg gaaaaagaaa cacaaaatgg
    19621 aaaagctgcg gttttagggc tgcagcatct gctagcgatg tatgcaggag cagtagcggt
    19681 gcctcttttg atcggtacag gactaggctt taatcaagaa cagatgactt atttgatttc
    19741 aattgatatc tttatgtgtg gattagcgac attattgcaa ttgacagtta accgattttt
    19801 cggaattggc ttgcctgtcg ttttaggatg tgcgatccaa gcagtagctc cactgatctt
    19861 gatcggaacc aatgaaggag taggagctat ctacggatct attatcgctt ctgggatctt
    19921 tgttgtgtta gtttctggtg tcttttctaa aattaaaaaa ctattccctc ctatagtaac
    19981 aggaacggtt atcaccgtta ttggtttgac tttgattcct gtagcaatcg agaaaatggg
    20041 tggaggtaat gcaacagcag ctgattttgg tgacaaaaca aatctgctac ttgcatttgt
    20101 gacgattctc ctaattgtag gcgtgcaaat gctggcaaaa gggttcattc gttcaatcgc
    20161 ggtattgatt ggtttagtcg gaggaagcat cttagctgct ttcatgggaa tgattgatct
    20221 gagcgcgatt ggagatgcgc cgttgttcca tgttccacaa cccttttatt ttggtaaacc
    20281 aactttcgac gtatggtcga ttttactaat gatcatcatt tccatggtca gcatggtaga
    20341 atcaacaggt gtatattttg cacttggtga cattactggg aaaaatgttg gagaagaaga
    20401 attgaaaaag ggatatcgtg cagaaggact tgccgttatc ttaggcggta ttttcaatac
    20461 attcccttat actggctttt ctcaaaatgt cggattggtt caactttcgg gaatcaaaac
    20521 aagaaaaccc atctatttct ctgcattctt cttgattgtt ttaggattat tccctaaaat
    20581 cggtgcgtta gcacaaatca tccctgagcc ggtacttggt ggaggaatgt tagtcatgtt
    20641 cgggatggtg gcagtccaag gaatccgtat gctgttggaa gttgatttca gcaatgataa
    20701 aaatctattg attgttgctg tttctgttgg atttgggctg ggtttcaatg ttacgccgac
    20761 attattcagt caaatgccgc aaacattgca aatgttcact ggaaatggca tcgtaatgag
    20821 tagcattacg gctatcatat tgaatttgat ctttaatgga ttgaaaaaag atgaagaaat
    20881 attggaagaa gccttcaaga agtaatagag caaaagaaag gttgtgaaat cagcgttttg
    20941 acctgattgt tagagaaaca aacggaaaaa tagttcctcg tattttgccg tttgtttgag
    21001 ctaataccga gggtcagctg attgaacacc gttgaaggaa gaggtcgtga gcctgccgtt
    21061 catctccgag tcacaaggaa caatttcaaa aaaaagcttc tcatgtttgt tgaatattgt
    21121 tccttgtgac cgaggagttg gcaggagaac accgtttatc cataaaaagg aatgtgacct
    21181 aacttttgtc acattccttt tttttatctt taataaacac gaacaataaa atgtttttct
    21241 ttgttaacat tcgaaatttc tttgacatca aaaaaagtga ttgttaaaat atgaatgtag
    21301 aaaaactggg aggaaaaaca atgacagcaa aaatctctgt gattatgggg agtacttcag
    21361 attgggaaac aatgaaagaa gcatgtcaaa tattggatga attcggtgtg tcttatgaaa
    21421 aaaaggtcgt ttctgctcat cgaaccccag atttgatgtt tgaatatgca caaaacgctc
    21481 gaaaaaaagg aatcaaaatc attattgcag gagcaggggg agccgcgcat ctgccgggta
    21541 tggtcgcatc aaaaacaact ttgccggtta tcggtgtacc ggtccaatca cgaacattaa
    21601 atggtttgga ttcgcttctt tcgattgtcc aaatgcctgg aggagtacct gtagctacta
    21661 cagcaatcgg aaaagcggga gcaattaatg ctggactact tgcgatacag atgctttcca
    21721 tgtatgattt agaattggag gctaaattgg ctgaaagacg ttcaatgttg gctaaaactg
    21781 ttatagaaag tagtgatcaa cttggttaaa cctttgttgc ctggttcaac gatcgggatc
    21841 gtcggtggcg gtcaattagg tcgtatgatg gcgattagtg caaaagaaat gggctttaaa
    21901 gtaggtgtgc tggatcctgt gagcgattgt ccagccgcac aagttgcaga ttggcatatt
    21961 gaaagtgtct atgatgacac ctttgcgctg aaagagcttg ccagaagaac agatgtcatt
    22021 acctatgaat tcgaaaacgt cagtgtagag gcgttaaatg cgattttacc catgtcattt
    22081 attcctcaag ggacagactt gcttgcgatt acgcaagacc gcctgttaga aaaatctttt
    22141 ttggaaacga ataacatcgt gattgctcct tatgcaacga tcgtcagtcc gacggatatc
    22201 caagatgcga ttgacgggat cggctatcct tgtgttttga aaaccactcg aggcggctat
    22261 gatggcaagg ggcagtatgt tttgaaaagt cgggcggatc tagctcctgc catggacctt
    22321 ttaagagaag gaacctgtgt tttagaagca tggatcccgt ttgaaaaaga gatttcgatc
    22381 atggtggcag gaaatggtca aggagatttc acgacttttc cggtcgtgga aaacatccac
    22441 cacaacaata ttcttcatga aacgattgct ccagccgcga ttgatcagga tgtgatcgag
    22501 gaggctgagc gtattgcccg tgtgattgca gaagcagtga gcctttcagg tgttttaggg
    22561 gtagaaatgt ttttaacaaa aacaggcggg ctatatgtga atgaattagc tcctcgtcct
    22621 cacaattctg gacattattc tattgaagct tgttctatga gtcagttcga tgcccatatt
    22681 cgaggtattt gtggttggcc attaggagaa aaagtacgtt tgataagcaa tgcagtaatg
    22741 gtcaatgttt tgggagaaca actgctggaa agctatcgct tgattgagaa taagccagac
    22801 tggcaattcc atttctatgg aaaaaaacaa gcaaaaaaag gtcgcaagat gggacatatc
    22861 actatcttga cagaagacat ccagcaaacg ctggaagaaa tcaaacaaac gaatatttgg
    22921 gattagagga gaaaaaacat gttagaacgt tatacacgcc ctgaaatggg gaatatctgg
    22981 acggaagaga acaaataccg tgcgtggctc gaagtcgaga ttttagcaga tgaagcttgg
    23041 gcagaacttg gtgaaatccc gcaagaagat gtgaaaaaaa ttcgtgagca tgcatcattt
    23101 gatatcgatc ggatcttgga aatcgaaaaa ttaacacgcc atgatgtcgt tgcgtttaca
    23161 cgagcggttt ctgaaacatt gggagaagaa agaaaatggg tccattatgg tctaacaagt
    23221 acggatgtcg tagatactgc ttatggctat ctcatcaagc aagcaaatga tattttacgg
    23281 gaagacttga aacgttttac ggaaatcatt ggcgaaaaag caaaagaaca caaatataca
    23341 gtgatgatgg gtcggacaca cggtgtccat gctgaaccaa ctacttttgg attgaaactt
    23401 gccacatggt attcagaaat gaaacggaac atcgaacgat ttgaacatgc ggcaaaagga
    23461 gtagaagcag gaaaaatctc aggagcagtg ggaacgttcg caaatatccc gccatttatt
    23521 gaagagtatg tatgtaaaca attggggatc cgtccacaag aaatttctac tcaagttctc
    23581 cctcgggatc tacatgctga atatttctca gcgatggcgc ttattgctac aagtattgaa
    23641 cgttttgcaa cagagatacg cggattgcaa aaatccgaaa cacgtgaagt agaagaattt
    23701 tttgcgaaag gtcaaaaagg ttcttctgct atgccgcaca aaaggaatcc gatcggttca
    23761 gaaaatatgg ctggtctagc cagagtggtg cgcggacaca tggtcactgc ctatgaaaat
    23821 gtcacattat ggcatgaaag agatatttct cattcttcag cagaacgaat catcttgcct
    23881 gataccacga ttttattgaa ttacatgtta aatcgttttg gcaatatcgt taaaaatcta
    23941 acggtctttc ctgaaaacat gaagcggaat atgtctgcaa catttggttt gatttatagc
    24001 cagcgcgtta tgctgaaatt gatcgataag ggaatgacaa gagaagaagc ttatgatctt
    24061 gttcagccaa aaactgcgta cgcttgggac aaccaagtgc agttccgccc gctgttagaa
    24121 gaagatccaa ccatcacatc ggtattatct aaagaagaaa tagacgatgc ctttgattat
    24181 catcatcatt tgaaaaacgt tgacgtgata ttcaaacgag taggtttgga ataaagaaga
    24241 gtgagttgca aaattcaatt aaaatagaat agcaaaaata accaaaaaag aagcagatcc
    24301 gttagcggtt ctgcttcttt tagtttatta tattttctta tcaataatag gaacctgatt
    24361 ttaaaagcgg ctacctcttt accgtgcctc tttacataca aaaaatagag gaattaagcg
    24421 taaatggctt ggctattata actaaaatgc tggttatgga gatgttgcag caagttttcg
    24481 agaaaagcgt attccatctc ttgtccattg aataagatct ggatacgata atgatttttg
    24541 aaacgcttct gttcatgaat ctgactgatt gaaagttcgc ctttgtgttc tgtctgataa
    24601 agaaaagtcc ttttacggca ttcagcacag cgcttggttg ctcggcggat ttttcggaat
    24661 gtataaaaac tagtatcttt attgcataaa taaacgtctt tgatcgacag caaatcatta
    24721 tatagacagg aacgttcttc agctgttaat tctgataaaa aaagctgacc ttgttttatt
    24781 gcatcttcca tcggcaagtg cctcctcgaa cccattaacc aaatttttag ttaattctaa
    24841 tataccatca ttaagtataa atgtaaacgc ttgttgatgg tataagattt tcgtttctga
    24901 taaaacacga acattaaaag aatctaatgc cttaaatgta catatttttg ttgatttata
    24961 aaaagatgat tgctaaaatt taaagtgaaa aaacgaacaa aaaacgatgt atttgctttt
    25021 aatgtaaata aaaaggagag acgtacatgg agaagaaaga acttttgtat gaaggaaaag
    25081 ccaaaaaaat atatcttact tctgatgaga acttgatttg gatagaatat ttagatcaag
    25141 caactgcgtt gaatggcgcg aggaaagacc agataagtgg aaaaggagaa ttgaataatc
    25201 aaatcacaag tgcaattttc cgttatctga ctcaaagagg aataaaaaat cattttattg
    25261 aacaattgtc taaaaccgaa caattagcaa aaaaagtaca aattatccct ctggaagtgg
    25321 ttatccgaaa tattgcagca ggaagtttct caaaacgtct aggtgttccc gaaggtaaag
    25381 aattcatcaa accaatcatt gaattctact ataaggatga ccagttggat gatccattta
    25441 tcaatgaaga gcatatctat ttcttacaac ttgcaaagcc ggaagaaatc agtgagctga
    25501 aagcccaagc gttggctatt aatgaaattt tgaccgaatt atttgctaag gtcggtttgg
    25561 ttttagtaga tttcaagcta gaatttggcc ggtcgaatgg acaaattttg cttgcggatg
    25621 aagtaacacc ggatacttgc cgattatggg atgaacgtac gaatgaacat atggacaaag
    25681 acgtttaccg cagagatcta ggtgatttga tcccagtcta tcaagaagta tataaccgtc
    25741 tatcggaact aactgaagaa aaggagcgag tatgatgtat ttcgtaaaag tatatgtaac
    25801 ctataaagat tcagtgttag atcctcaagg ggaggcagta aaaaaagcag tccatcgtat
    25861 gggatatgaa acaatcgaag atatccgaat aggaaaatac tttgagatac aacttgctga
    25921 tgcagaagaa ccaatcgata cagtgattga aacgatttgt gacaagctgc ttgcaaatgt
    25981 aaatatggaa acataccgat atgaaatcaa aaaaatggag gaagtataac atgaaatttg
    26041 cggtcatcgt ttttccaggt tcaaattgtg acgcagatat gttatgggca gttcgcgaga
    26101 tcatgggcgc tgatgccgaa tttgtacggc atgatgagtc ctctttagaa ggatttgatg
    26161 gggttttact gccgggagga ttctcctatg gtgactattt gagatgcggt gcgattgctc
    26221 ggttttctac aatcatggaa gaagtgattc gttttgctga agaagggaaa ccagtattcg
    26281 gtacatgcaa tggattccag atattgacag aagcaggact tttgccaggt gtactgagac
    26341 gaaatgagtc tttgcatttt atttgcaaaa ctgtaccatt gaaagtcaac cccaagacgg
    26401 cctttacttc agagtatgaa gccgacgaaa taatctatct tccagttgct catggggaag
    26461 gaaattattt ctgtgacgaa gaaacacttc aagaactaaa aaaaaataat cagattgcct
    26521 ttacttataa tgacaacatc aacggcagcg tggaaaacat tgcagggatt acgaacaaag
    26581 cagggaatgt gttaggaatg atgcctcatc cagaacgtgc agtagaagac ttgctaggat
    26641 cagaagatgg gaaacggttc tttgcttcca ttttaaaaaa cttcggaaag gtgaccgtat
    26701 agatgaaaac attgaaagaa ccgacagctg aagagatcaa gaaaaaaaga atctatgccg
    26761 aatggggatt gactgatgaa gaatatcgaa tgatcgaaga agatatctta ggaagaatgc
    26821 ctaattatac ggaaactgga ctgttttctg ttatgtggag cgaacattgt tcctataaaa
    26881 attctaaacc ggttctacgc aaatttccta ctagtggacc tcatgtgttg caaggccctg
    26941 gagaaggcgc aggcatcgtc gacatcggtg atggactagc agttgtcttc aaagcagaaa
    27001 gtcacaatca tccttcagcc gtggagcctt atgaaggagc tgcgacagga gttggcggaa
    27061 tcatcagaga tattttcagc atgggggctc gtccaattgc gttgctagat tcattgaggt
    27121 tcggcgaatt agataatcca agaacgaaat atttactaga agaagtagtt gcaggtatcg
    27181 gcggatacgg caattgtatc ggcataccga ctgtaggcgg cgaaattgca tttgatcctt
    27241 gttatgaagg aaacccatta gtcaatgcaa tgtgcgtagg tttgattgaa cacaaacata
    27301 tccagaaagg gcaagccgct ggcgtcggta attcgatcat gtatgtaggt gcaaaaacag
    27361 gtcgcgacgg tatccacgga gcaacttttg cctcagaaga atttaatcaa gaagaagaac
    27421 agcaacgttc tgctgtacaa gtaggcgatc cttttatgga aaagctgctg cttgaagcgt
    27481 gtttggaatt aatcttgcat cattcggatt ctctaatagg tatacaggat atgggagcag
    27541 caggacttgt ttcatctagt gcggaaatgg cttcaaaagc tggctcagga cttatattga
    27601 cattagatga agtcccacaa agagaaactg ggatgacacc ttatgaaatg atgttatcgg
    27661 aatcacaaga acggatgttg atttgtgtta aatcaggaga ggaagaaaaa atccaagaac
    27721 tatttcagaa gtatgatcta gatgcagtca ctatcggtaa agtaacagac gacgggcaat
    27781 atcggctata tcatcaagga gaagaagtag caaatctgcc agtcgatgca ttagctgaag
    27841 atgcaccagt ttatcataaa gaaatgaaag aaccagctcg aatcgtagaa tttcagcagt
    27901 tagctcctta tcagccagtt atcgaagaac ctggacaagt attgcttgat ctgctgcagc
    27961 aaccgacgat tgcttcaaaa cgttcaatct acgaaactta tgattcacaa gtacaaacca
    28021 atacagttgt ccgtccagga agcgatgctg ctgtgatgag ggttagaggg acgaataaag
    28081 cgttagctat gaccacagac tgcaacgcgc gctatctgta tttggatcca aaaatcggag
    28141 gacaaatagc agtggcagaa gctgctagaa atattattgc tagcggtggt aagcccttgg
    28201 cgatcaccga ctgcctgaac tatggttctc ctgataaacc agaagttttt tgggaattat
    28261 cgacttcagc tgacggaatt gctgctgcgt gtgaaacgtt aggaactcct gttatttcag
    28321 gaaatgtctc tttatataat gaaacggatg gtcaagcaat ctatccaaca ccaatgattg
    28381 gtatggtcgg attgattgag gatcataaac atatcactac tcaggaattc aaaaaatcag
    28441 gggatctaat ctatatatta ggtaagacgt ttgcagattt tgatggcagt gaattacaaa
    28501 agatgcagct tggaagaatc gaaggtgtga tccgaaactt tgatctatcc attgaaaaga
    28561 gaaaccaaga gttagtattg acagccatcc aaaacggatt gattgaaagt gctcatgact
    28621 gttcagaagg tggcttggca attgcactgg cagaatcagc tttcaaacat cagctaggga
    28681 tatctgtaca gtttgaattg tcctctgctc aattatttgc agaaactcaa tcaagatttg
    28741 tgttgacagt cgcaccggaa aataagacac gattcgaaga aatgatggga gatgctgctg
    28801 ttttagcagg taaggtgacg gatgaagcga taatcgaaat ttcagcaact gacggtcaga
    28861 tcaaaataga aactgcagta gcaagaaagt gctgggagga tgcaatcgta tgtcttatga
    28921 agtgaagagt ttaaatgaag aatgcgggat cttcggtgtt tggggtcatc cagacgccgc
    28981 tagtgtggca tacttcgggc tccatagtct tcagcatcga ggacaagaag gtgcaggtat
    29041 cgtgtccaat gatcgaggaa agcttaaggg gcatcgagat ttggggctgc tttctgaggt
    29101 attcaaagat caaagaaaat tgcagcagtt aacaggagaa gctgcaatcg gacatgtacg
    29161 ttatgcaaca gcaggcaatg gaagtgtgga caatattcag ccttttctat tcaaatttca
    29221 tgatcagtcg atcggcttag cgcataatgg taacttgatc aatgctcgtt cccttagaaa
    29281 agagttagag gaaaatgggg cgatcttcca ttctaactcc gatacggaga tattgatgca
    29341 tttgatccga cgaagtgaag aaacagattt tttaaataaa ttaaaaagtg ctttgaatga
    29401 agtcaaaggt ggctttgcct atttattaat gacagaaacg gcaatgattg ccgctttgga
    29461 cccaaatggt tttcgtccat tagcaatcgg acaaatgaaa aacggagcat acgtgattgc
    29521 ctcggaaact tgtgcattag aagttattgg ggcacgtttt ataagagatg tccagcctgg
    29581 tgagatagtg atcatcaatg acgaaggaat ccagattgat tgctttacga aggacacgca
    29641 gctttctatt tgttcgatgg aatatatcta ttttgccaga ccagattcaa acatcgcagg
    29701 gatcaatgtg catactgcac ggaaaaatat gggacggaat ttagcgaaag aatcgccagt
    29761 aaaagcagat atggtcgttg gcgtacctaa ttcttctttg tcagccgcaa gcggttatgc
    29821 ggaagctagt ggaatccctt atgaaatcgg attagtaaaa aatcaataca ttgcccgaac
    29881 atttatccag ccaacgcctg agctaagaga gcaaggtgtt cgaatgaaat tatctgctgt
    29941 acgaggagta gtggaaggta aaaaggtgat tatggtcgat gattcaattg ttcggggtac
    30001 gacaagcaga cggatcgtcc atcttttgaa agaagcaggg gcaaaagaag tccatgtgag
    30061 gattgcttct cctcctttga aatatccatg tttttacggg attgatattc aaacgcgtaa
    30121 agaactcatt gcggcaaatc acacgattga tgaaatcaaa gaatgtatcg gtgcagattc
    30181 tttaggtttt ttaagtgaag agggtctgat cgaatcgatc ggtctggaaa aagatgctcc
    30241 gtattcagga ttgtgtatgg cttatttcaa cggagattat ccgacgccgt tatatgacta
    30301 tgaagaaaaa tatcaggaat cactaaaaga aaaagtatcg ttcttttaaa agcagaccaa
    30361 gaagggagta tatgaattca atggcaaatg catacgcaaa agctggcgta gatgtcgaag
    30421 ctggatatga agtagtagaa cggatcaaga agcatgtcaa aaaaacggaa cgtttaggta
    30481 tgatgggcgc tataggagga ttcggcggtt gttttgattt atcggtcttt caattaaaag
    30541 agccagtcct tgtctcgggg accgatggcg tggggacgaa attagcagtg gcaatcgaag
    30601 cagacaaaca cgatacaatc gggatcgact gcgtagcgat gtgtgtcaat gatatcttag
    30661 cacaaggcgc agagccgtta tatttcttag actacttagc aacggggaaa aatgatccgt
    30721 tacgtttgga aaatgtagta aaaggtgtag cagatggatg tgttcaagga aatatggcgc
    30781 tgattggcgg agaaacagct gaaatgcctg gtatgtactc aggtaatgat tatgatctag
    30841 ccggatttgc tgtagggata gctgaaaaat ctcaattatt gacaggagaa acgatcaacc
    30901 caggagatgt gttgcttgga atagcatcta gcgggattca ttcaaatggg tactctcttg
    30961 ttcgcaaaat tttctttgag cagcagcagt tttctttatc atcaaaactg ccagaattga
    31021 aaaaaacact tggcgaagaa ttgcttgagc caacgaaaat ctatgtttct tccttgcttc
    31081 cgctgatcaa agaaaagaaa ataaaaggag ctgcacatat cactggtgga gggtttgtag
    31141 aaaatatccc gcgaatgtta ccagaatcta tggctgcaca aatcaaactt ggctcatggc
    31201 cgattcttcc aatattcggc tgtttacaaa aatatggtga aattcctgag atggagatgt
    31261 atgaaatctt caatatgggc atcggtatgg tcctggcagt cgaagaatcg aaagtaaaag
    31321 aaatagtaca cgaaattgag caagctggcg aaaaagttta tgaaatcggg aaagtgatcg
    31381 ccaaagaaaa acatcgtgtg gtgtttatag gagaagaacg atgagaatcg ctgtttttgc
    31441 ttctggaaac ggaagcaact ttcaagcgct agccgattat ctatcgaaaa aaggattgga
    31501 atcttcgatt gactggttgt tttgtgatca gcctgaagca tatgttttga aacgtgcaac
    31561 tgcgttgagt gtaccggcag attgtttttc acctaaagaa tttgattcaa aaaaagagta
    31621 tgaagaagca attttgcata aattgaaaga aaaaaagatc gatttgatcg tattagctgg
    31681 atatatgcga atcattggac cggtattgct gaaaaattat gataaaagaa tcatcaatat
    31741 ccatccgtct cttttacctg ccttccctgg tttgcatggc atacgtgatg cctttgaagc
    31801 tggagtaaaa gaaacgggtg tcacaattca ttatatcgac caaggtgtag atactggacc
    31861 aatcatccgg caggaaaaag tgagaattga acaggaggac acgttcgatt ctttagaaga
    31921 aaaaatccat agagtagagc atcgaatcta tccagaagtg attagtgaaa ttattgaaaa
    31981 tggaggctat ctgaaatgac aagagcattg atcagtgtat cagataaaac tgggattgta
    32041 gcattcgccc aaggtttgat tgaacaagga gtagaaatca tttcaacagg aggaacaaag
    32101 catgcgttgg ataaagctgg gatcaaaacg ctttcgatcg aagaagtgac agggtttcct
    32161 gaaatgatgg atggccgtgt caaaacactt catcctaaaa tccatggtgg tcttttagga
    32221 agacgcgatc tccctagcca tatggaggca atggaaacgg aaaatatcca accaatcgac
    32281 tttgtttgtg tcaatttata tcctttcaaa gaaacgatta tgaaaccaga tgtcgaaata
    32341 gcagaagcta ttgaaaacat tgatatcggt ggtcctagta tgctccgtag tgcagcaaaa
    32401 aaccatgaat ttgtcacctc tgttgttgat ccatctgact atgaagaagt actttcagaa
    32461 ttacgtcagt atgggcatac ttcactttct ttcagacaaa aactagcggc aaaagtcttt
    32521 cgtcatactg cggcatatga tgcactgatc gctcaatact taacggattg ggtggaagaa
    32581 aaagaaccgg aaaaattgac acttacgtat gaacgaaaac aaacgcttcg ttacggtgaa
    32641 aatagccatc aagccgctgc cttttatcag tccgctcttc tggaaaaata ttcgatcgca
    32701 gcagcaaaac aactccatgg gaaagaactt tcttataata atatccgaga tgcagatgct
    32761 gcgctacgga tcatgaagga atatgacgag ccaacggtag tcgccttgaa acacatgaat
    32821 ccttgtggta ttgggacagc atctacgatt ttagaagcgt gggaagcagc gtatgaagca
    32881 gatcctgttt caatctttgg tgggatcatc gttttgaatc gagaagtaga tctgccaaca
    32941 gcagaacaga tgcataagct gtttctagaa atcatcattg ccccttctta tacacaagaa
    33001 gctttggaat tactgcaaaa aaagaaaaac atccgattac tgacagtaga ctttgcgcag
    33061 gaagaaaatc aaaatcgaga agaaacggtc tcagtcttag gtggacttct tgtacaagat
    33121 caagatctgg caatagaaaa tgaagaagag tggaaagtcg tgacaaaacg taagccgact
    33181 ccagcagaag caaaagcatt agtgtttgct tggagagctg tcaaacatgt gaaatctaat
    33241 gcgatcgtac tggcaaacga acgtcagaca gtaggaatcg gagccgggca gatgaatcga
    33301 gtcggttcag taaaaattgc gattgaacaa gcgcaagaga aaatggaagg tgcagtttta
    33361 gctagcgatg cttattttcc aatggatgat tcggttgaat atgcagccaa acatggtata
    33421 aaagcaatca ttcagccagg aggaagcatc aaagaccaag attctattga tatggcgaat
    33481 aaatacgatc tagcaatggt ctttactgat gttcggcatt ttagacatta gagaggagtc
    33541 ggataaatga acattttggt gatcggtgca ggtggtcgtg aacatgcaat aagtaaaaaa
    33601 cttttagcca gtccgaaggt ggatacggtg tattgtgcgc caggaaatcc ggggatgaca
    33661 aaagatggta tcaattgtgt cgctatcagt gagcgagatc attcagcgtt aatccatttt
    33721 gttaagaaag aggctattga ttggagtatc gttggtcctg aagttccttt gttaaatggg
    33781 atcgtcgatg attttatggc ggaaggattg aaaatcttcg gtccgacaaa agcagcagct
    33841 ctgatcgaag gttcgaaaag ttttgctaaa caattaatgg atacttatca aatcccgaca
    33901 gctaaatcca gaagttttga atgttacgaa aaagcagcag cgtatatcaa agaaaatggt
    33961 gcgccaatcg tagtgaaagc ggacggctta gcagctggga aaggtgtagt cgtggctacc
    34021 acagtgaagg aagcgctgga tgcggcagaa caaatgttga accagcatcg atttggggat
    34081 agtagcaaaa aaatcgtcgt agaagagttt ttggcaggag aagaattctc ccttttggcg
    34141 tttgtccgta ataccaaggt gtatccgatg gtcatttcgc aagatcataa gcgggcattt
    34201 gataacgata aagggccaaa tacaggcgga atgggtgctt attcaccagt accgcaaatt
    34261 tcaaaaaaga tgattgaaaa agctgtggaa gaagttttaa aaccagcggc tttgggaatg
    34321 aaagaactag gacgctcttt cacgggtgtt ttatatgcgg gtttgattgc tacgaaggaa
    34381 ggaccaaaag tgatcgaatt caatgcgcgt ttcggtgatc ctgaaacgca agtcgttctt
    34441 tctcggttga agagtgactt tgcgcaagta atcgatgatt tattagagaa tcgtacagta
    34501 gagttaaagt ggcagcaaga aggatacaat gtaggcgttg tggttgcagc tgccggttac
    34561 cccgaagaat atgagacagg aatggttcta ccagattttc tggaagaaga gctttctgtt
    34621 tactatgctg gagtttcctc aaataacggc aaactagttg gagcaggagg acgtctttat
    34681 ttagtagaag catacggtga gacgatcaaa gaagcacaga aaaaaattta caccgcgtta
    34741 atggataaaa cgacagatgg aacattttat cgtacagata ttggagaaaa agctgccaat
    34801 agcgaactga tcgtgaagta aatcagctgc ttccatttat aaaaaataca acaaacataa
    34861 tcggaagcgc cgattttagt gaagcctttt cataaagagt gtggtacact tttgttgtgt
    34921 atataaataa ggaggagttc agatgaaaaa aattcaatta ggtacttctg atcagaaagc
    34981 agctgcagtg atcttaggct gtatgcgtat caacaatgcg gaagatccag taaaagtgat
    35041 tgagacagct tatgaaaacg gcattgattt ttttgaccat gcagatattt atggaaacgg
    35101 tgaatgtgaa gaaatctttg cagatgctct agcaaaaaca tcaatcaaac gagaagatct
    35161 ttttattcaa acaaaatgtg gaatcgttcc aggtgtgatg tttgattttt ctaaaaaaca
    35221 tatcattgaa tctgtagaaa aaagtttgac ccgtctgaaa atggattatg tggatgcttt
    35281 acttcttcat cgtccagata cattggtaga accagaggaa gtagctgaag cgtttgatga
    35341 attggaacgg tcaggaaaag ttcgttattt tggcgtcagc aatcaaaaac caatgcaaat
    35401 cgaattgctg aaaaaatatg ttcgtcaacc attagtagct aatcagctgc aatttggctt
    35461 gaaacatact ggaatgatcg atcaagggat tcatgtcaat atgacagatg aagcaagtat
    35521 cgatcatgac ggtagtgtac ttgattattc ccgattgcat gatatgacga tccaagcatg
    35581 gtcaccatac cagtatggat tttttgaagg tgtatttatc gggaacgaaa aattccctga
    35641 attaaaccaa acattagaag aactagcagc taaatatcaa acgacaccaa ctggcttggc
    35701 ttctgcttgg attttacgcc atccagccaa tatgcaagtg atcgccggta cgatgaacgt
    35761 agggcgaatc aaggaaatcg ctcaagcttc agaaattaat atgtctcggg aagattggta
    35821 cgaattgtac cgtgctgctg gaaacatttt accttaaaga ttgtcataag caggtcaaaa
    35881 gggctataaa ttaaaaagac agaataactt gaaagctgtt ttcagcttcg agatattctg
    35941 tcttttttta ttgcaaatgg aaagagtatc attgtacgga tttcgtttat tagttatttt
    36001 tgtttttaag gaaaagctgt gtccgatatt cttttggaga aatcccttgt tttttcttaa
    36061 ataaataact gaaatagtta gggtcattat aaccaatttc aaaagcaata tctgaaatac
    36121 gatagtctga ttgctgcaat aatttttttg ctaattcgat tctttgttct gtcagataat
    36181 cgatgaatgt ttggttaaac gattgagaaa aaatcgtact gaaatgagag ggacttaatg
    36241 caacttgttg agcgacccaa gttaaggaca tatctggatc ggtaaaattt tctttgataa
    36301 aatccaatgc atggttcaaa attggctgat atttgttcct tgaaggctgt gagggcagag
    36361 cagttaagaa agaaatgaac atataaactg tcgaacggta aagcgagtcg tcggcgctga
    36421 ttgctaagcg ttgactggtg gatgtgcaat gaaccatttc ttgcgggatt ttcttttctt
    36481 tcaggcagac atgctggagt agcttagtca agacaaataa acgatataat cgtgttcgag
    36541 ttggtgtgcc ttgtggtttc gtcaagtgtt cgatgtactt agatacttcg tttttcgata
    36601 atgaagacaa atcaagtgag aggtcaaagt aaaagacaga atcaactgga agtttcgctt
    36661 cttgaatcaa atcctcatag ctgataattt gttctttaag ggaactggcg gtttcaagta
    36721 tcatctgcgt gatctcaaaa gatgcaggta attcgctgat tctttctgcg acaggaccaa
    36781 tggcgacaga aaaattattt ttttgtccct tttctaattc atggaccaac gtttgggcta
    36841 cctgataact ggtctcgatc acattttctt caagagaatc aactattaaa aatttgatga
    36901 atcgagagga aacagcagag aagatcactt gttgattctc cctaaataaa aacttcaaat
    36961 aatcactgag atgagcgtaa tcttcaaagt ctgtactgac ttggtttgct gctaataata
    37021 cggtcatttt tttccctgct aaagagcgat tgaaagattc agcaagtttc aatgtttcac
    37081 tgatcgtaag tgagcctttg aacaaaccgt tcaagaaatg attttttttc aattcgaaaa
    37141 caagatcaga agaagtgtgc gtttcttttt gctgcaaatc taaagcagtt actgcttttt
    37201 ctattgcttg tttcaattcg ctacttttaa tcggtttaag cagataatca tctgcctgta
    37261 cctggattgc tcctttcata taatcgaaat cgtcgaatcc actaataaat atgagacgag
    37321 tccaaggaag tagtttcctt gcttcttttg aaaaagtcaa cccgtccatg aaaggcattt
    37381 ggatatcagt cagaatgatg tcgggctgta agtcgataat tgaagacagt gccatctcac
    37441 catctccagc ttcgccgaca taagtgattg gataataaga accaaggaca gttatttgtt
    37501 cccgcaagtt attgcgaatg aggtattcgt cttcgatgat aaaaacttta tacatgtttg
    37561 tttcctccaa ttgttttggg agatagaggg atcatcagtg tgacagttgt tccttctcgg
    37621 tatttgctgt caatcgtgat aggtttttga tcatttccgt aataaagaag gagacgcttg
    37681 tttacgttgt aaagtccgta gccgatactg aaatctgaat cgatcccttt ggctaactct
    37741 ttacgtattt ctgccagttt ttctgcaggg atgccaatcc cgttgtctat aactttgaat
    37801 tggatcatct catcaatttt gcatgtggta atacgaataa atcctttgtg acggacgtac
    37861 ttcgttccat ggtatacagc attttcaatc aatggctgca agatcatttt taaaatagaa
    37921 acaggaaaaa gtgtttctgg tatatcgatc ttatagtcaa gcatatggcc gtatcttatt
    37981 tttaagatga ctagataatc attgatatgg ctaatttctt tttcaagagg aatccagtct
    38041 tgtcctttgc ttaaagacgt acgaaaaaag ccggaaagtg catgggtcat agtcagagct
    38101 tcttcaaagc agtcttgttc gatcaaagaa ataatggcat ccaaactgtt ataaataaaa
    38161 tggggagtaa tctgagcttg caatacacga acttcacttt gtgctaaatg gtattgtttc
    38221 aaagcgttct cccgtaaaga ctggtttaga tcttccgcca ttttattcat gctagcggta
    38281 agtgtttgta attccagtgt ttccggcagg gttaaacgaa agccaaggtt ccctgaagaa
    38341 agttgttctg acatctcgat cagctgattg atcggttctt ggacctttct ctctaaaaag
    38401 cgacgattct ttttcgcata gaaaaaaatg gtactaagaa ctccaagttc cactattgtt
    38461 aatagaatga gcgagcggac aagtgtcgta ttttttttgc ttgctaagtc gatctccaca
    38521 cgaacaaaat cttgtaagat atctgacagc agttgagtga cagaatctac ctgattgatg
    38581 atctcttcat tggtatccat aaaatctttt gccaagatat tattgatcag ctcgttttga
    38641 taatcttcca gtgtatcgat cgtacgtagc gatacatcta acgtactaat ttctttaagt
    38701 gtatttgtat tatttttgct ctctcgaata tcattcctaa gttcttctag gatattttcc
    38761 tcattgaact catctacggt tacttggcca gaaatcaagt cccacatact ttccaaaact
    38821 ttttgatcaa ccttcaaagc aattgcatct gctttttgga tatttgcgat cgtctgttga
    38881 taaatcaaaa gctgttgcga atataacagt atgctcagta gcaaagggat aacagcaaat
    38941 agaagcatgg ctttgtttgc ttgggcaaaa atctgtttta tgcttaaatt ttttgtcatg
    39001 atacttctcc agtcaataat ttctggttgg gatgttagct atattctgct gggaaaagac
    39061 agtctcggaa ataaaaatat ctttaggtaa tgtttttcct gtttgaatat ttccaaaata
    39121 acgagtaacg gtattggcta cataccagcc gacatcaggt ttacactcga ctacacaatt
    39181 gacgattcct gagcgtagct tctcgatcat atcttcttgt ccatcaatag ttacaatgat
    39241 caaatcttct ccaggtttga ttgatgtttc ttttatcgca gctaaagcac cgtgagtcat
    39301 ttcatcacta tggctgtata atacatcgat ttcggataaa tcatttgttt ctaaatatcg
    39361 tttcatctgg tcttttcctt tcatccgaat aaaatcaccg acaattcggt gaacaatagt
    39421 catcggaggt ttatggtcga tggttccatc tttttttagg gttggtttac gattgattga
    39481 ttccaaaaat ccttcggagc gtagcttggt cggtgtggag ttctctgaac cagcaagttc
    39541 taggattctg atttcctgtt tctttgaatt tgtgaagtga ttgctgacaa ataaacccgc
    39601 ccgatttcct tctgctttga aactggggcc aatgtgggtg acgaaaagct cgggatcatt
    39661 cgtttgaata tgtctgtcga caagtatgac agggatccct gctcgctttg cttcttttaa
    39721 tacagagtcc cacccggatt cttgcaaagg agtaaaaatg atcatatcca ctttataggc
    39781 aatgaacgtc cgtatatctt ggatttgccg ttcctgattc atgtagccat ttcggtagag
    39841 aacttggtaa tcttcttttt caagcgcagt gataattgat tcagtatgct ttttccgcca
    39901 gctactctcc gttcctgatt gcgaaaaacc aacgactagt gggtcaggtg cttcctttgt
    39961 attaggtgta caccccatca tttggtaagc gattacaaca agggctaaaa aaaataaaac
    40021 acgtttcact ttaaatcact ccatctggat ttgttagtac tatcttatca gataaaaggg
    40081 aaaatcaaag tcaaaaggaa tcaatcgaaa aaaatccaag aaacttaaaa aaatccaaga
    40141 caaaaaaagt acacctaaaa aatttaaagt aaatcccata aaaaaatcaa tgttaacgct
    40201 ttctttatta gctatactcg gtgtatcaat tgaaatgggg gataaaaaat gaaaaacaaa
    40261 tttagaaaga ttgttgggac tatcgctatt ttgaccgcaa gcattctatt aagtgcgtgt
    40321 ggcaattcag gatctgcaga tgatagtacg gggtatgtag gaatcgctat gccgaccaaa
    40381 tcagcagaaa gatggatcgc tgatgggaac aacatggtct cggaattaga aaaactgggt
    40441 tataaaacag atcttcaata tggagaagat aaagtagaaa atcaagtagc acaaatagaa
    40501 aatatgatta caaaaggggt agatacactt gtgattgctt ctatcgatgg ttcggcttta
    40561 actgatgtgt tagctaaagc aaaagaagca gatatcaagg tgatcgccta tgatcgttta
    40621 ctgatgaatt cagaaaatgt cgattattac gcgacttttg ataattttgg agtaggtgta
    40681 tcgcaagcag cttatattga agagcactta ggattgaaag aaggaaaagg accattcacg
    40741 attgaattat ttggtggctc gcctgacgac aacaacgcac taatcaatta caacggagtg
    40801 atgtctgttt tacaaccata tatggacaac ggtcagttgg tcgtgccatc tggacagacg
    40861 agtttcagtc aaatcgctac gcttcgctgg gatggttcta cggcgcaagc aaggatggat
    40921 aatctgttaa gcgcaaatta tactgaccaa acattggatg ctgttttgtc gccatatgat
    40981 ccaataagtt taggaatcat ttcgtcatta aaaggcgtgg ggtatgggag tgaaagtaaa
    41041 cctttacctg tgattactgg acaagatgcg acagttgctg gcgttaaatc gatcattgcc
    41101 ggggaacaaa cacaaacgat ctttaaagac acacgtatct tagcaaaaaa tacgattgaa
    41161 atgatcaaag caatcagtga tggagaagag gttccggtca atgatactga aacttatgat
    41221 aatggggtga aaactgtccc tacgtattta gcaaatacag tatctgtcga taaagataat
    41281 tatcaagcag agctgatcga caccgattat tataaagaat ccgatctgaa aaattaagag
    41341 gaaccaaaca aggaactaaa gagatctaag tcagagagag gataagaatt atggcggatt
    41401 gtattttaaa aatggcagat atcaccaaaa aattttctgg aatccgagcg ttagattgcg
    41461 tgaatctgat gattgaacgt ggggaaatcc atgcattgtg cggagaaaac ggagcgggaa
    41521 aatcaacatt gatgaatgtc ttgagcggct tgtatccata tggctcctat gacggggaaa
    41581 tcttttatga tggaaagctg tgtcaattta aagatttgaa agatagtgaa gaaaaaggca
    41641 tcgtcatcat tcatcaagaa ttagcactaa gtccttatct ttcaataaaa gagaatattt
    41701 ttctcggaaa tgaacaagca gaaagaggcg tgattgattg ggaactgaca gaaaagaaaa
    41761 catatgaact gctgaataaa gtcggtttgt ctatcgaccc gaatcttcta gtatcccaga
    41821 ttggcgtagg gcaacagcaa ctagttgaaa tagctaaagc attatcaaaa tccgtccgtc
    41881 tgttgatttt ggatgagcct accgctgcat tgaatgaaga agaaagcgcg aatttgctga
    41941 gattgatccg agagttgaaa gagcaaggtg taacctccat cattatttca cataagctga
    42001 atgaaatcgt ggatatcgca gatcgaatca ccattttacg ggatggacag acgattgaga
    42061 cactagaaaa tgaaatgatc agcgaagagc atattattcg cggaatggtg ggcagagatt
    42121 tagttgatcg ttatcctgaa agaaaaccgg atattggaga agtttatttt aaagtagaag
    42181 actggacggt ctatcaccct cttgatcacg agcgaatcat aaacgaccac atcaacttgc
    42241 atttgaaaaa aggagagatt gtaggaatag ctggactgat gggagcgggc agaactgaat
    42301 tcgctatgag tctatttgga cattcttatg gaagcaagat atccggacgt atattaaagg
    42361 aaggggcaga gatatcagtt aagaccgttc cttcagccat tgcaaacgga ctcgcttatg
    42421 tctcggagga ccgaaaatct ttaggactga atctgctgat ggatattcgg gaaaacgtga
    42481 cgctgagcag tttggataaa ataagtcaaa aaggagtatt agataaagaa aaagaagtaa
    42541 ctgaggcaga aaatttccgg aaaaagatgc gtataaaaac aaactccatc catcaaattg
    42601 tcagtagtct tagtggtggg aatcaacaaa aagtcgtttt atccaagtgg ctgatgacac
    42661 aacccgatat tctttttttg gatgagccaa cccgtgggat agatgttggg gcaaaatatg
    42721 agatctacac gattatcgaa gaaatggcag caaatggcaa agctgtctgt cttatctctt
    42781 ctgaacttcc tgaaatatta gggatgtgtg atcgaatcta tacgatgaac gaaggacgta
    42841 ttacaggaga agtctcgcga gaagatgcag atcaggaagt cttgatgaaa ttaatgacga
    42901 aagaagaggt gggttaagtt ggaacaggca aaaaatctta cggataaaaa agagaaaaaa
    42961 ctaaatttga aaagtagagc agctgatctt tttggtcgct acagcatggt gatcatttta
    43021 gcgggactac ttattgcgtt tcaattgatg acggatggta ttttctggcg tccattgaat
    43081 ataactaacc ttgttctgca aaatagccat atattagtac tagctgcagg gatgttatta
    43141 gttgtgctct taggtcatgt ggatttgtcg gtagggtcag tgatggcgtt tgttggtgca
    43201 attgcgggca tgatgatggt caatcgccaa atgaacccgt ggatgacagt tatcgcatgt
    43261 cttgcagtag gcgggctgat cggcgcatgg caaggttttt gggtagctta tgcagggata
    43321 ccggcgttta tcgtgacttt agcaggattg ctgatgtttc gtggactgac acaagttgtt
    43381 ctaggaggcc aatctctagc accttttcca gctgttttcc aaaaaatgtc tacgggttat
    43441 ttaccagact taagcgaagg aagcagcctt cacttcttga cattgatttt aggaatgatc
    43501 atcacgatcg tgttagttgt tggtcaatgg cgaacaagaa atcgtcggaa aaatcattta
    43561 tttgaagtgg aatctccaac agctttcttg gtaaaggcgg tatttacagc agtcgttgta
    43621 ctaggaataa cttatatctt tgcctcttat caagggtatc cgattatttt gatcattctt
    43681 ggtttgatcg ttgcagcata tgggtttttg acaaataaaa cagtagcagg tcgacagatt
    43741 tatgcgacgg gcggaaatcg aaaagcggct gaactatcag ggatcaaaac aaagcgaatc
    43801 acgttctggg tattcgtcaa catgggagtc atggcagctt tagctggatt agttttagct
    43861 gctcgattga atgcggcaac acctcaagca ggtacatcga tggaactgga tgcaatggcg
    43921 gcagtatatt ttggtggtgc ttcgacttcc ggcggcattg gaacaatcat gggtgcgatt
    43981 gtcggtggct tggtcatggg tgtattgaac aatggcatgt cgattttagg cgtcggggta
    44041 gactggcagc aggcaatcaa aggattgatc ttgcttttag ccgttgtgct agacttgtat
    44101 aataagaaaa aacgaacaac gtaggagaac gccggttatt cagaaaaaag actgtgacaa
    44161 gacatttgtc acagtctttt tttcgctaaa agcgaacatt cattgtttcg tttgtataga
    44221 ctaaaaataa tatatgtttt tttaccattc gtataaattc tctttttgct cttgattttt
    44281 ttgcttaatt gagttaaatt acatagggcg ctaaaaaaag ccttgattaa attaataatg
    44341 ttcgtgtttt gctggatgtg aacatcagga ggataatgta atgaaagtat ttgattacga
    44401 agatattcaa ttaattccta ataaatgtat cgtaaatagc cgttctgaat gtgatacaac
    44461 ggtcacatta ggtaaacata cattcaaaat gccagtagtg cctgccaaca tgcagacgat
    44521 tattgatgaa acaatcgcag aattcttagc ggaaaatggt tatttctata tcatgcatcg
    44581 gtttgatgaa gcagctcgaa ttccgtttat caaaaaaatg aaaaagcgtg gattgattac
    44641 ctcgatcagt gttggcgtca agaaagagga atattccttt atagagaagt tggcagaaga
    44701 atcgctaaat cctgattata tcactatcga catcgcgcat ggacatgcta actcagttat
    44761 tgatatgatt cagcatatta aaaaatattt gcctgaaact tttgtgattg ccggtaatgt
    44821 tggtacaccg gaagccgtcc gtgaattgga aaatgcgggc gcagatgcaa caaaagtcgg
    44881 gattggtcca ggaaaagtat gtattacaaa aatcaagaca ggctttggga ctggaggctg
    44941 gcaactggca gctcttagat ggtgtgcgaa agcagctcgc aagccgatca tcgctgacgg
    45001 tgggatcaga acacatggcg atattgcaaa atctgtccgt tttggtgcga caatggtcat
    45061 gattggttca ttgtttgccg gtcacgagga atctcctggc gaaacaaaag tcgaaaatgg
    45121 aatcgtgtat aaagaatact ttggttctgc ttcagaattc caaaaaggcg aaaaacgaaa
    45181 tgttgaagga aagaaaatct ggatccaaca caaaggatct ttgaaagata cactagtgga
    45241 aatgcaacaa gaccttcaat catctatttc ttatgctgga ggaagagatc tggaagccat
    45301 tcgaaaagtt gactatgtca tcgtgaaaaa ttcgattttt aatggagaca caatctaaaa
    45361 aataaataaa atattaggat gttgtgaaaa agttgtcctg cattaagtaa taagaacagc
    45421 caaacaaaaa tagttcttta tatgttttgt ttggctggaa ttattatcgc agatacaagc
    45481 ttttgaacat cattattcag aataagggac tgtgataaga tttttgtcac agtcccttat
    45541 gttttgcaaa aaaagttttt cttttttctc gtaaattcgg taaaatcaaa agaaaaaata
    45601 gacaatagaa aagagaagct tatgtgggaa aaagtagaga aaaaacaagg accgatttat
    45661 cgtcaaatta tggaacagat tatgagagaa atcgaaaacg gaaggatcaa tcctggaagt
    45721 caacttgaat cagaacgaaa actagggcaa ctttttggtg tgaatcgttc caccgttgtc
    45781 catgcattgg aagagcttag ggagcttggg gtactaacga gcaaacgtgg cagcggatgg
    45841 tttgttaatc agactgaatg ggggaaattc gctgttccaa gaatcgattg gcggcaaatg
    45901 atctctcctc gttatgagca gacggactgg tatgagcaaa aaataagaga agcaaagcag
    45961 atccaaaaaa catcgtttct tgatttatac gctagcgaga tgccagatga gcttttgccg
    46021 aattttgagt ttccttctta ttctcttgaa gaaatcttag cggaagaaaa agaaatggat
    46081 cttttaggct atactccgtt acgtaacaaa atcaaacggt atttggaaag taaatttgaa
    46141 ttggcatttt cccccaacca gcttcttgtg actggtggcg gtcagcaagc aatctttttg
    46201 attttacaaa cgattctttc agctggagag gggatagctg tagagtcacc ttcttttttt
    46261 taccgattac ctcttttcaa agcttcagga acgcgtttat ttgggatacc gatggatgaa
    46321 gaagggatcg acttagaaaa actgaaagaa tcgatccata aaaataaatt gaaagcagtg
    46381 cttatcaacc cgaattttca aaaccctaca ggaaaagtga tgagtcaaaa acgacgagaa
    46441 aagctagtag aattgtgtcg acagtatcgg cttccaatta ttgaagacga tgtatttacg
    46501 gatctttcat ttctaaacca agaagaagtc cttcctatac gaacgattga tccggataat
    46561 gtgttatacg tcggttcgtt atctagggtt ttaggaaaaa caacgaaaat cggttgggtg
    46621 ataggacctg agacttttat ctcgcaacta gctcaagcgc aagaaatgat ggagttttcg
    46681 atgaatatcc ttacacaaat cacagtggct aatgtattta atgaagcaat tgatttgaaa
    46741 atgcaagatg taagaaaaaa gttgaaagaa aaaagccgac tgataagcgc ttggggagat
    46801 tcacaatcgt ttttcaagct tcattcaatt ttaggtggtt actatgcctg gattacttgg
    46861 gatgggaaaa aactagaccc cgaactagca gaaaaagtag tagatatggg tcttggaatt
    46921 gcaccaagtc tactttttgg gcaagatata aatggaatca gaatcaatta cagtaggctg
    46981 gatgaaaacc aaataccgtt gttttattca aagatgaaaa ttctagaaaa gtggttaaca
    47041 gaataatttc acaaagtaaa tagattaaag aatataacag cttggattta aatttcaagc
    47101 tgtttctttt ttttcggaaa aagaatacga tataaagaaa ctaagaaaaa accattattt
    47161 gtacgtccgt tcacgatgtg taaataaaat aaatgtttgc aacaaaactg tataacacca
    47221 acgtttttaa atagaaacag atggttgtaa gtttgattag aaaatagaaa aaatgcagat
    47281 gaaatataaa acgctttcct tattgtgatt ttattcacta atgctgttaa tatgcggatt
    47341 ggcataattt cctttcatcg tgaaaatttg tgcttttagc ttgtatttct cataaatcca
    47401 tgataattta ttaatgaaga cggttaaata gtgatacagt tttattcact gtttcaatcg
    47461 cagaatgaag agattttctt atagatgaga gaggaatgtg tgacaaatgg caaacaaaaa
    47521 agcaccgatc gacttccaag cactgctaga tgaagtcaat gcagatttcc cagtatatca
    47581 agcacttgat caagatggaa acgtagttaa caaagattta gtacctgatt tatccgatga
    47641 tgaactcgtt gaattaatga ctcatatggt atggtctcgt gtattagacc aacgttcaac
    47701 agcgctgaac cgccaaggac gcctaggctt ttttgcacca actgctggac aagaggcaag
    47761 tcagctggct agtgcttacg catttgataa agaagatgtt cttcttccag gttatcggga
    47821 tgtacctcaa ctagtaaaac atggtttacc tttatctcaa gcttttttat ggtctcgagg
    47881 acatgctgca ggtaattctt atccggaaga attgaaagct ctaccaccac aaattatcat
    47941 cggtgcacaa tatgtacaag cagccggtgt tgcattaggt ttgaagaaac gaaacaagaa
    48001 aaatgtcgtc ttcacttata ctggtgatgg cggttcatca caaggtgatt tttacgaagc
    48061 aatcaatttc gctggcgctt accatgcaaa tgccgtattt tatatccaaa acaacgggta
    48121 tgcgatctct acacctcgtg aaaaacaaac tgcagcaaaa acacttgcac aaaaagctgt
    48181 cgcagctggg attcctggta ttcaagtcga tggaatggat ccgcttgcag tatatactgt
    48241 atctaaaatg gcacgggaat gggcgatcag tggaaatgga ccagtcctaa tcgaaacact
    48301 gacttatcgt tatggaccac atacactatc tggtgacgat ccaacccgct accgtacaaa
    48361 agacacagat gacgattggc agaaaaaaga tccattgatc cgcttccgta aatatttgac
    48421 ggaaaaagga ctttggtctg aagaaaaaga agaacaagtc atcgaagcaa ctaaagaaga
    48481 aatcaaagca gctattgctg aagccgacaa agtaccaaaa caaaaggttt cagatttctt
    48541 gaaaaacatg ttcgaagtat ctccacaatc aatcaaagaa caaattgcaa tttatgaagc
    48601 gaaggagtcg aaataatcat ggcacaaaaa acaatgatcc aagcaatcac agatgcctta
    48661 gcacttgaac tagaaaatga cgagaatgtc gttgtttttg gtgaagatgt tgggaaaaac
    48721 ggaggagttt tccgggcaac tgaaggattg caggaaaagt tcggagaaga ccgagtattc
    48781 gatactcctt tagctgaatc tggaatcgca ggattgagtt tcggtcttgc gttagaagga
    48841 tttcgtccag ttcctgaaat ccaattcttt ggttttattt tcgaagcaat ggacgaagta
    48901 gtagctcaaa tggctcgtac aagatatcga atgagcggca ctagaaatct acccatcaca
    48961 attcgttcgc catttggtgg cggtgttcat acaccagaat tgcattcgga taatttagaa
    49021 ggtttgatcg ctcagtctcc tggtattcga gtagtgattc cttccaaccc ttatgatgca
    49081 aaaggactat tgatttcttc tattagaagt aacgatccgg ttgttttcct agaacacatg
    49141 aaactatatc gctcattccg agaagaagtg ccggatgaag cttacgaagt tcctttggat
    49201 aaagcagcag ttactcgtga gggaacagat gtatctatta tcacatacgg agcaatggtg
    49261 cgtgaagcaa tcaaagcagc agacaatctt gcaaaagaaa atatatctgt tgaaatcatt
    49321 gatcttcgta cagttgcgcc attagatgta gaaacgatca ttcaatccgt ggaaaaaact
    49381 ggtcgcgtag ttgttgtcca agaagcacaa cgacaagcgg gcgttgccgc gcaagtagtt
    49441 tctgagatct cagaacgcgc tatcctttca ttagaagccc caatcggacg agtttctgca
    49501 cctgatacag tcttcccatt tggacaagca gaaaatgtct ggttgccaaa tgcaaaagat
    49561 atcgaagaca aagtaaaaga aatcgcggaa ttttaagaaa agaagggacg aaacaaagtc
    49621 atggcttatc aatttaaatt gccggatatc ggtgaaggaa tcgcagaagg tgaaatcgtc
    49681 aaatggttcg tcaaaccagg agatacgatc aatgaagatg atacattact agaagttcaa
    49741 aatgacaaat cagtagaaga aatcccgtca cctgttacag gaacggttaa aaatgtgatt
    49801 gttccagaag gtaccgtagc aaatgttggg gatgtattag tagaaatcga tgcaccagga
    49861 cacgaagaca atgaagggga cagtggtgta gccgcagaat cccaaactcc agctaaaccg
    49921 gctgctgaac ccactgttga tacagaatca gctggatcgt caagcgaagg agtcttccaa
    49981 tttaagttac cggatatcgg tgaaggaatc gcagaaggtg aaatcgtcaa atggttcgtc
    50041 aaaccaggag atacgatcaa tgaagatgat acattactag aagttcaaaa tgacaaatca
    50101 gtagaagaaa ttccgtcacc tgtcacaggg acagttaaaa acgtgattgt tccagaaggc
    50161 actgtagcaa atgttgggga tgtattagta gaaatcgatg ctcctggaca caatagtgca
    50221 ccgtcaacta gtgcgccatc agctgaagca ccaaaagaaa aagtggaaac atcaggttca
    50281 gcaagtgtcg ttgaagctgc cgatccaaat aaacgtgtat tggctatgcc atctgttcgt
    50341 caatttgccc gtgaaaaaga tgtagatatc tcacaagtaa ctgcaactgg aaaaggtggc
    50401 agagtaacaa aagaggacat cgaaaacttc ttggcaggtg gtccaagcag tgcaccagcg
    50461 aaatcagaag cgccagaagc ggcagcaccg aaagaagcag ctcctgcagc agaaagcaaa
    50521 ccagctgcac cagcaaaacc attcaagtcc aacttaggag acttggaaga acgtgtagca
    50581 atgacgccaa cacgtaaagc tatcgctaaa gcaatggtca acagtaagca tacagctcca
    50641 cacgtgacat tgcatgatga agtagaagtg tctaaactat gggataatcg gaaacgattt
    50701 aaagaagttg ctgctgcaaa tggtacgaaa cttactttct tgccatatgt cgtgaaagct
    50761 ttaacagcga ctgttaagaa atacccagta ttgaatgcat ctatcgacga tgcaaaccaa
    50821 gaaatcgttt ataaacatta ctacaatatc ggtattgcaa ctgatacgga tcacggacta
    50881 tatgtaccaa atgtgaaaga tgcagaccgc aaaggaatgt ttgctattgc agacgaaatc
    50941 aatgagaaag caaaattagc tcatgacggc aaactatctg cagaagatat gcgcaatgga
    51001 acaattacga tcagcaatat cggatctgta ggtggcggtt ggtttacacc agtcatcaac
    51061 tatccagaag tagctatttt aggtgtagga acgattgcac aacaaccaat cgtaaatgct
    51121 gaaggcgaaa tcgttgtcgg tcgtgtgatg aaactgtcat taagtttcga tcaccgtatc
    51181 gtagatggag caactgcgca acaagcaatg aataatatca aacgtctttt agctgatcca
    51241 gagctattaa tgatggaagg atgataaaca aatggtagtt ggagattttg ctgtagaatt
    51301 agatacagtt gtaattggtg cgggacctgg tggctatgta gctgccatcc gcgctgctga
    51361 aatgggacaa aaagtagcaa tcattgaaag agagtatatt ggcggtgttt gtttgaatgt
    51421 cggctgtatt ccttcaaaag cactgatagc tgcaggacac cattatcaag agtcactaga
    51481 ttctgaaatg tttggagtaa ctagtgaaaa cgtcaaacta gattttgcaa aaacacaaga
    51541 atggaaagaa aacaaagttg tccatacgtt gacttctggt gttggtttcc tattaaaaaa
    51601 acataaggtt gaaacaatcg aaggagaagc gttcttcgta gatgatcaca cactacgcgt
    51661 cattcatcca gattcagctc aaacttattc gttcaataac gcaatcatcg caacaggttc
    51721 ccgtccaatc gagattccag gatttaaatt cggaggacgt gtgttagatt ctactggcgg
    51781 attagcattg aaagaagtac ctaagaaatt tgtgatcatc ggtggcggtg tgattggtgc
    51841 cgaattgggc ggagcgtacg caaacttagg agcagaagta acgattcttg aaggctcacc
    51901 acaaatttta ccaacttacg aaaaagatct agtaaaatta gtagaagatg atttcaagaa
    51961 aaaaggcgta acagtcgtaa cgaatgcaat ggcgaaagaa gcagtcgata acggtgacag
    52021 cgtgacagtg aaatatgcag tggacgggaa agaagaatcc gttactgctg attatgtgat
    52081 ggtcactgta ggtcgtcgtc cgaatacaga tgatatgggc ttagaacaag cgggtgtcga
    52141 agtaggagaa cgaggattga tcacggtgga taaacaagga cgtacgaacg tacctaacat
    52201 ctatgccatc ggtgatatcg ttccaggggc ggcattagca cacaaagcca gttatgaagc
    52261 aaaaatcgct gctgaagcaa tctctggcaa aaaagtagct gtagattata aagcaatgcc
    52321 ggcagtagcc ttcactgatc ctgaattggc ttctgtaggt atgacgatca aagaagcaaa
    52381 agatgctggt atcgaagcaa aagcatataa attcccattt tcagggaacg gtcgggcatt
    52441 atcgcttggt aaaacagaag gattcatccg tctagttacg acgatcgaag ataacgtgct
    52501 tattggcgcg caaatcggtg gagtcggcgc aagtgatatg gtctctgaat tagctttagc
    52561 aatcgaatca ggaatgaatg ctgaagatat cgccttgacg attcatcctc atccatctct
    52621 tggcgaaatc gtcatggatg cttcggaatt agcattaggt ttaccaatcc atatttgatt
    52681 tgaataatac aggcgaaacg caactgaaaa atcagttgcg ttttgttttt tagaattatc
    52741 aataatatta ttatgtaaac ttaaaaatat gacaacttct tggtttatta ccacttccta
    52801 tgctacactg aagaggaata tgaaatagga gagttgaaga tgcaagaata tcagtatcca
    52861 cttgatttag attggacaac agacgaaatg gtcattgtaa tgaagatgtg ggaagatctg
    52921 gaacgtgcga atgaaaaagg gataaacaat gaagcttttt tgaagactta tcagcagttt
    52981 aaaactgttg tcaaatccat tggtgaggaa aaacgattgg gacgggaatt tgaaaaagct
    53041 tccggttatt ctttatatcg tacagtgcag caagcaaaaa aaaatccaaa caaaactttg
    53101 agaatgaacg ggtgagataa atggaacaga tgattgatca tatcaagtcc tggcttgaag
    53161 aagctggtga gaaaatcaaa tcttctcaac aaacattaac aatctcgcaa aaatcaaatc
    53221 gaacggatct agtaacaaat gtcgatcgaa acatccagaa ttttctaatc gaaaaaatcc
    53281 attcctttga tccagaggcg aaaatattgg cagaagaaga agggtgtaat caattatcgg
    53341 atctcgatgg aagagtcttt atcatcgatc ctattgatgg caccttgaat tttgtgattg
    53401 aaggggaaaa tttttgtatt atgctcgctg tttatgaatc cggcgttgga aagcttggtt
    53461 tcatttatga cgtgatgaga aatgaacttt tttggggtgg aaaaggaata ggcgtgtata
    53521 aaaatgatga gccattgcct agaccagcaa ataaatcctt atcggaaggc ttacttggtg
    53581 tgaatagtta tcttttcggt cataataaat atggaatccg agaaattgcg gaaagcagca
    53641 tggggacacg tatgtacgga tgcgctggtc ttgaactagt ggcaatcttg aaaggaaacc
    53701 acattgctta tatttcaaac cttagccctt gggattatgc agcgggaaat gtcttattgg
    53761 aagagtttgg tatggtaaca agcggtcttt ttggtactcc tttgacattt gaaaaaaggg
    53821 aatactattt agcagggact tcttgtgcat atgcagaaat cgcagacctt ttgaaaaaag
    53881 aaggctgaaa aaaaggtaga aatcattcac aaaaatgata aaaacgagca aaagattaat
    53941 tttctcgaaa aaatgaaaac attttactat atttcatgag aaaagtctgc tataattagc
    54001 agtcgagtaa aatttgccct gtcataaaga accaataaaa aaggagttag ttacattgaa
    54061 ttatagaaac gatatccgta acgtcgcaat tattgcccac gttgaccacg gaaaaacaac
    54121 attagttgat gaactattaa aacaatcaga tacattagac gcacatacgc aattgcaaga
    54181 acgtgcgatg gactcaaatg cgctagaaaa agaacgtggt atcacgattt tagccaaaaa
    54241 cacagctgta gactataaag gtattcgcgt aaacatcatg gatactccag gacacgcgga
    54301 cttcggtggc gaagtagaac ggatcatgaa aatggttgac ggcgttgtgt tggtcgtaga
    54361 tgcgtatgaa ggaacaatgc ctcaaacacg ttttgtattg aaaaaagcac tggaacaaca
    54421 tatcacacca atcgttgtcg taaacaagat tgacaaacct tctgctcgtc ctgagcatgt
    54481 cgtagatgaa gttcttgaac tattcatcga attaggagca gatgatgatc agttagactt
    54541 tcctgtgatt tatgcatctg cactaaatgg tacgtcaagc ttgtcagatg atccagcaga
    54601 tcaagaacca accatggcac ctatttttga tacaattatt gaaaaaatcc ctgctcctgt
    54661 cgataacagc gacgaaccat tacaattcca agtttcatta cttgactaca acgattatgt
    54721 aggtcgtatc ggaatcggac gtgtatttag aggaacaatc aaagtaggag accaagtagc
    54781 attaattaaa ttagacggaa cagttaagaa attccgtgta acgaaattat ttggtttctt
    54841 tggactaaaa cgtctagaga ttcaagaagc aaaagctgga gacttgattg ctgtctcagg
    54901 tatggaagat atctttgtcg gtgaaacagt tactcctgtg gatcatcaag atgcgttacc
    54961 tatccttcac attgacgaac caacattgca aatgactttc ttagttaata attcaccatt
    55021 tgccggtcga gaaggtaaat tcgtaaccgc tcgtaagatt gaagaacgtt tgatggcaga
    55081 acttcaaaca gatgtatcct tacgagttga accaacaaac tctccagatg catggacagt
    55141 ttctggtcgt ggagaattgc atttgtctat cttgattgaa aacatgcgcc gtgagggata
    55201 cgaattgcaa gtatctcgtc cagaagttat tgaaaaagaa atcgacggtg taaaatgcga
    55261 accgtttgaa cgcgtgcaaa tcgatacacc agaagaatat atgggtagtg ttatcgagtc
    55321 cttgagctta cgtaaaggtg aaatgcaaga tatggtccat acaggaaacg gacaaatccg
    55381 tttgacattc ttaacacctg cccgcggttt gattggttat tcaactgaat tcctttcaat
    55441 gacacgtggt tacggaatca tgaaccatac ttttgaccaa tatctgccaa tgcttcctgg
    55501 tcaaattggc ggacgtcacc aaggggcgct tgtttcgatc gatacaggaa aagcaacgac
    55561 ttattctatc atgagtatcg aagaacgcgg tacagtcttc gtagaaccag gtacagaagt
    55621 atatgaagga atgattatcg gggaaaacag ccgtgataat gatttgactg tcaacatcac
    55681 gaaagcaaaa caaatgacaa acgtacggtc tgctactaaa gaccaaacat ctgtcatcaa
    55741 aaaaccaaaa caattgacgt tggaagaatc tttggaattc ttgaatgatg atgaatattg
    55801 cgaagtaaca ccagaaagca ttcgcttgag aaaacaaatt ttagagaaaa atgctcgtga
    55861 aaaagcatct aaaaagaaaa aataatctgt aaaaggatga agagagaaac cagcttgtta
    55921 taaaaaatag ctggtttctt tttttgtcca actcttttct gtggagaagt catctttgtc
    55981 aatggacata aattcagaaa atacgccgaa agacgtttaa aagactttta aaaaacttat
    56041 ttctagagag tttttgtttc ataataagat aatgtttcac gaaaggagat caacttgtcc
    56101 ttcgaagaaa atcgaagtaa aatggcaaga gaaaggagcg ggaagatgaa cgacttatta
    56161 aaacaatact ttggctatga tgagtttaga cctggtcaaa aagaaattat ccagaaagtc
    56221 attgatcagg aaaatgtatt aggaatcatg cctaccggaa gtgggaaatc aatctgttac
    56281 cagcttccag ctttactatt agatgggctt actgtggttg tgtccccttt aatctctttg
    56341 atgaaggatc aggtggacgc ggcgaatcaa ttaggtatac ccgccacttt tatcaatagc
    56401 tcgttagatg gttacgaaac agctagacgc ttccaagaaa tcgatagaca gcagtaccgt
    56461 ttactttata tcgctcctga acgttttatc atgccggatt ttatccaagc aatgaagcga
    56521 tggaacgtcc gtatgatcgc tattgacgag gcacattgta tctctcagtg ggggcatgat
    56581 ttccgcccaa gttatttaca gatggcgaac cagctagatc aactgccaaa tcgtcctgta
    56641 attgttgctt tgacagctac agcgactgtg caagtagctg ctgacataaa aagactttta
    56701 aaaattccag aaaacaacca tattcagact ggttttgaac gagaaaatct acgatttcaa
    56761 gtaattaaag atcaaaaaaa agaacaatat ctaatcgaat atttaaagat caacaaaaat
    56821 caatctggta tcatttatgc agcaacgaga aaagaagtag accgtctcta tcatttgctt
    56881 agaaaattcg atttttccgt gggtagatat catggtggtc taaatgagaa tgagcgaaca
    56941 gaaatgcaag aagccttttt atacgatcgc ttgcagctga ttgtagcgac aaatgcattt
    57001 ggtatgggta taaataaaag caatgtccgt tttgttatcc attatcaaat tcctggctct
    57061 ctagaagcat actatcagga agctggtcgg gcgggaagag atggtctttc cagtgaagcc
    57121 atcctgcttt tctctcctca agatatccaa gtccaaaagt tttttatcca gcagtcccaa
    57181 agagaagaag ggcaaaagca aaaagaatat gaaaaattaa aagcgatgac agaatacgtg
    57241 tatattgagt cctgtcttca gcaatacatc ctaaactatt ttggcgagac aagcagtccg
    57301 tgcaaccgtt gtggtaactg tttagatgac agagaaatag tagaagtaac gacagaagca
    57361 cagatggttc tttcttgctt aaaacgtatg ggtgagaact atggtaaaca aatgctgatg
    57421 aaagtcttag caggatcgaa agaacaaaag ctgcaagctt taggttttgg acatttatct
    57481 acctatggac tgatgaaaaa ccaatcgcaa aaagaaacga tgcagttaat tgaatatttg
    57541 atatcgaatg gctatcttct gaccatcaat ggtgagtatc ctgttttgaa agtaacagaa
    57601 agagggatac aagtattgaa aggacaagaa tctgtttatc gaaaggaacc caagaaagtc
    57661 cagcagctat cggatgaaga aaccgataca ctatttgaag tgctgcggga actgcgaacg
    57721 gatctagcat cagaagcagg cgttcccccg tatgtcgtat tttcagattc cacattgaaa
    57781 gaaatgagcc gaatacgtcc atcaagcaga ttggagatgc ttcagataaa aggagttggc
    57841 caaagcaagc ttgataaata tggcgaagcc tttttatctc ggataaaaaa tgcggactct
    57901 aacgttctgg ataagtagaa agaacgaata cgtaaaaatg aataaaaaca actggttaaa
    57961 tctgtttttt ttgctcctta tgaaaatcaa atatgttata attaaggaat tagaatggga
    58021 ggtaagcaca gatggattct ttgtcgaaaa aacttgcgat ccaagtatta aatgatgaag
    58081 cagaacggat caaacgatta atccgaaatc aaaacaacag tctctgtatc tcacaatgca
    58141 aagccttcga agaagtggta gatacacaga tgtacggctt ttcccggcaa gtcggttttg
    58201 ctgtccgtat aggagtaatc ggaaagaacg agggccagct gatgttaagt gagctagaac
    58261 gagaattgaa tcagttatat agtgaggttt accaagaaat ttatgataaa aaagaagttg
    58321 gcaaggagga atagacttgc ctaagaaagt gaaaaaaagg catctattag actatagcat
    58381 cttgattccc tatttaattt tatgtgtatt gggattgatc atggtatata gttctacgtc
    58441 gtatctatta ttagaaaacg gacaaaatcc gtccgcatca gtcattaacc agagtatttt
    58501 ttgggtgctt agcttgattg ctatcgcact actttataaa atgaaaacag atgttttgaa
    58561 aaaccagcgt ttgatcatgg cagccatcgc agttttgacg attttgctgc tgattgttgt
    58621 gttttttggt aaggagatca atggggcgaa aggttggctg caaattgcag gattctcgat
    58681 ccaacctgcc gaatacctaa aaatcatttc tatctggtac ctgtctctga ctttatctaa
    58741 aagacaaaac agtgtacaaa aagattttct tgggactgtg aaacgtccac ttgcaatggt
    58801 tatcggactt actgcattag tcgctattct tcccgatttt ggtaatgcgg cagttatctt
    58861 tttgattatt ttggttctgc ttttagccag cggagtcaat tatgtgtata ctttgattgt
    58921 aggagtagga ggcttttgtc taagtacttt tacgatctgg ctgatcaata tcacaaacgg
    58981 aaagatctta cccggacgtt tacagtatat ctataatcga tttgctattt accaaaatcc
    59041 tttctcagac gaattaaata aagggcatca actagtcaac ggctactatg ccatgttcaa
    59101 cggaggacta tttggcaggg gattaggtaa cagtatccag aaaaaaggat ttttacaaga
    59161 agcacaaacc gattttattt atgcaatcgt agtggaagaa ctgggagtga ttatggggat
    59221 tttgatatta gcgttgctat tctttatgat tgtgcgcatt gtccttgtag gtatccgttc
    59281 aaaagatccg ttcaattcgc ttttgtgcat tgggatcggc gcaatgtttt tgatccaagt
    59341 atttgtcaat cttggaggga ttactggcgt tattcctttg acagggatca cgttcccatt
    59401 tttaagtcaa ggaggctcca gtttgctgat gctttcgatt tgtgtcggct ttgtcctcaa
    59461 catcagtgcg gatgaaaaac gcaagagctt aggtttataa taaaaaaaga tgtaggagtg
    59521 gttcgatgaa aaaagtttta gttgctaaca ggggtgaaat cgctgtacgt gtatttcgtg
    59581 cttgtacaga attaggaatt aaaactgttg gaatctatgc agaagaagat gaatattccg
    59641 tacaccgttt caaagcggat gaagcatatt tagtaggaca aggaaaaaaa ccgattgatg
    59701 cgtatctaga tattgaaggg atcatttcta tcgcgaaaga atgcggagct gacgctattc
    59761 atccgggtta cggtcttttg tcggaaaact tgaattttgc gcaacggtgc gaagaagaag
    59821 gcatcatttt tgttggaccc aaactgcatc atttagatat ttttggagat aaaatcaaag
    59881 caaaagctgc agctatcgaa gcggggattg cttcgattcc aggcacagat ggaccaatcg
    59941 cttctataga cgatgccttg gagtttgcga aacaatacgg ttatccgatt atgatcaagg
    60001 cagctctagg cggcggcgga cgcggcatgc gtgtagctca cgatgaaaag agtgcaagag
    60061 agggctacga acgtgcgaaa agcgaagcaa aagcagcctt tggtagtgat gaagtgtatg
    60121 ttgaaaaata catcgctaat cctaagcata tcgaagtaca gattctcggg gatacgcatg
    60181 gaaacgtcat ccatcttttt gaaagagatt gttctgttca gcgccgtcac caaaaagtag
    60241 tagaagtagc gccttgtgtc tctatgaatg aacagcaacg tcaaaaaatc tgccaggcag
    60301 ctgtccaatt gatgaaatat gtcgggtatg tcaacgcagg tacagtagaa tttcttgttg
    60361 aaggagatga tttttacttt atcgaagtca accctcgcgt ccaagtggaa catacgatca
    60421 cagaaatgat tacagatatt gatatcgtaa caacgcaact gttgattgct caaggtttgg
    60481 atctgcataa agaaatcgga ttgcctcagc aagaaggaat taaattaaat ggttcagcca
    60541 ttcaatgccg aatcactaca gaagacccgt tgaataactt tttaccagac acagggaaaa
    60601 tcgatacgta tcgttcacct ggtggttttg gcgtacgtct ggatgtcgga aatgcttacg
    60661 cgggctatgt agtgacaccg tattttgatt cattgctagt aaaagtatgt acgcatggtg
    60721 ctacctttga aacagccatt cagaaaatgg aacgttgctt gaaagaattt agaatccgtg
    60781 gagtgaaaac aaacattcca tttatgctca atgtcatcac acatcctgaa ttccaatcag
    60841 gaaatgcaaa aacaaccttc attgatagta cagcaacatt atttgaattt cctcgactac
    60901 gagaccgcgg aaacaaaacg atgaaatata tcggtgaaat tactgtgaat ggttttccag
    60961 gtatcgaaag tggcgaaaaa ccattttatg aagaaccacg tatgcctaaa gatctaatca
    61021 caagaactga ttacgtaacg gctaagaatg tattagatgc gaagggtgcc gatgctttgg
    61081 tcgaatggat caaaggacag gaaaatttgt tgttgacgga tacgacattt agagacgccc
    61141 atcaaagtct gctagcaact cgtgttcgga caaaggattt caagcagatt gcacgtttga
    61201 ctggagaagg tcttcctgaa ctcttttcaa gtgagatgtg ggggggagca acatttgatg
    61261 tggcttaccg ctttttgaat gaagatcctt ggcaacggtt gagaaaaatt cgttctttaa
    61321 tgcctaatac gttgctgcaa atgcttttca gaggttcaaa tgccgttggg tattctaatt
    61381 atcctgacaa tgtattggtg gaatttgtca aagaagcagc agcgcaagga atcgatgtat
    61441 ttcgaatctt tgatagtttg aactggactc cacaaatgga aaaaagtatc caagctgtac
    61501 gggatactgg taaaatcgca gaggcagcta tttgctatac tggcgatatc aacgatccaa
    61561 gtcgggcaaa atacaatgtt cagtattata aagacatggc aaaggaacta gaacaactgg
    61621 gtgcgcatat cattgcaatc aaagatatgg ctgggctgct taaaccgcag gcagcttatc
    61681 gtttgattag tgaattgaaa gaaacgacag atctgccaat ccatctgcat actcatgata
    61741 ccagcgggaa tggaatcatc acttattctg ctgcttcaaa agcaggagta gacatcgttg
    61801 acgtggcaat gagtgcaatg agtgggaata ctagtcagcc tagcatgagc agcttgtatt
    61861 atgcactggt caatggtcca cggctgccag aaatcacgat tgaaaatgca cagaaattaa
    61921 accattattg ggaagatgtg cgcatgtatt acaaaccgtt tgaaaacggc ttgaatgctc
    61981 ctgaaacaga agtatatatg catgaaatgc caggaggaca gtattcgaat ctacagcaac
    62041 aggcaaaagc tgtcggatta ggtcatcgct gggatgaaat caaacaaatg tatcatactg
    62101 tcaacttgat gtttggcgat atcgttaaag taactccatc atctaaagta gttggagata
    62161 tggcactatt tatggtccaa aatgatctta cagaagaaga tatttatgaa aaaggcgaaa
    62221 cactaagttt ccctgaatct gttgtgactt tcttccaagg agaactaggg caacccgttg
    62281 gcggatttcc taaaaaattg caaaagatta ttcttaaagg cagacctgca ttgaatgaac
    62341 gtccaggtct tttggctgaa tcagtcgact tcaacgaagt aaaaaaagaa ttggcagaaa
    62401 aaattggcta tgaaccaaaa caagacgaag tattaagcta tttgatgtat ccgcaagtct
    62461 ttttggacta tcaaaaagca tataatcaat ttggcgatgt tacattactg gacacaccaa
    62521 catttttcca aggtatacgt ttgggtgaaa caatcaatgt acaaatcgaa cgcggaaaaa
    62581 cgcttatcat tcgtttagat gaaatcggag aacccgatat cgaagggaat cgcgtactgt
    62641 tcttcaattt gaatggacaa agacgtgaaa tcactatcaa agataattcg atcatcagta
    62701 cggtccaaac ccgattgaaa gctgaaccga caaaccgaga acaaattgga gcaacgatgt
    62761 ctggttctgt tttggatgta ttagtgaaaa aaggcgataa cgttaaaaaa ggtgatacgt
    62821 tgatgattac tgaagcgatg aaaatggaaa cggccatcga agcacgtttt gacggagaag
    62881 tcgcacatgt atatgtgtct tcaggagaca ctatttcgtc tggtgacctt ttgattgaag
    62941 tgactgagaa ataataagcg aaaggtgggg aaacatgaaa agactgggtt tgttttggct
    63001 gattctgcta ttagttattg tggcattcta catggaacca gtcttgtttc cgccaaaact
    63061 tgaagtgaag gaaaactctg atcaacaaat atcaaaccaa atgacaacgt ggaaatacca
    63121 ggagcttaaa gcaactggtt tttctactta tatcggaaaa ccgatcgatc agctggaaaa
    63181 ggattttggc caagcctatg atcgattaac tagcggattt ggttttgaaa caagatatta
    63241 tgaaaataaa gctagtcatc tcacatttga agcgaacatc caagacagca gagtcacaac
    63301 tgttaaagta ctgcaaacag ggcctgctga tatcgcgccg ttttatttag ggatgacgat
    63361 gcaagatctt acgaaaatca ctactatcta tactaatttc acttttgagt accatgatac
    63421 agaagttgga atcgaactaa tggaagagga tatgaattat cgtccgctaa ttgcttttga
    63481 taacgacact tttgctattt tattttttga tcaaacaaca aacggcctat tttcagttgt
    63541 gtatttagat aaagatagtc ttttgaaact attgccatat caagtgtttg gtgaaggcct
    63601 tccacattat caagcagata aatcggctga ttgggaatca atcaatcgcg ttaaagaacg
    63661 aaaaagtacg actttattaa atatgcttag aagagaagac gaacttccca tatacaatca
    63721 aacacttgcc tttcaaaacc acagtcagct gctactacat gattttttga caaggcctga
    63781 ggaaatattg tcagaggaac gtatggctga ttggcaaaaa tcgctaggca gtgctcaaac
    63841 gacagtgaaa ttctcactga cgaatgatga attggagaag ttaatcagta aacaaaaatt
    63901 agaaaatact agtgggctat ttattcatcc ggcgattgac gctacttttt cgtttttata
    63961 ttggtacagc gatccttaca accatgaccg tttcatggac ccgggaacgg attctgtagg
    64021 cattgctttt tctaaagaaa atatgttagt cttattgcaa gaagaaaacg gagaaagtag
    64081 tgatagcagt gatcgttaac gaagagctgt ttcaactgga ggatcaatgc ctccaagtcg
    64141 ctgaaatgat aaaaaagagt aaaacgatgg cttcttatct tgaacataaa cagtcaatgg
    64201 aagaatctgc aaaagtttgc cagttgaaag aagcgttctt gtctaagaaa gaagcatttg
    64261 aaaatgtagc ggcatatggt gagtatgcac ctgattatcg tgaaaaaaaa cgagctgttc
    64321 gaaaagcaaa aagacagctt gatatggatg agcatgttgc agctttccga tatagtgaaa
    64381 cacagctgca aagtctgttg gatcaaattg tccaagtact tgccgaagaa gtttctaatg
    64441 aaatcaaagt agatgcaggt agcccgtttt ttgaaacgaa aaaacacttt ggatgcggag
    64501 gtaattgtca tgcaattaga caatagtgaa tttgaaataa caaaacgacg cgggctgatc
    64561 gtatgggtct acagcttaaa acaattaaag aatctgaaaa agttcggctt gttgcattat
    64621 gtctcaagaa gaatgaaata tgtcgtgctt tacttaaatg aagagacatt ggaagaaaca
    64681 gaagaacgaa tccgtaaatt acattttgtt cgaaacgtag aacgttctta ccgaccggat
    64741 atcgaaatga attttgccga aaaaatagga aacaaaacag tattaaaaga cgaaggtttc
    64801 gaaattgaag agctaaatac agaaatccga ttagctgaac atctttaaaa atctcttatg
    64861 agcagttttt aaataaaaaa taacttggat aacgatcgat ttttgatcat tgtccaagta
    64921 tttttttggt atactagtac cagtaccatt tctaaggagt gagtagatgc ggataatatc
    64981 aggaaattat ggcgggcgcc gtttaaaaag tttagcaggg gcgaacacca gaccaactac
    65041 agataaagta aaagaatcca tattcaatat gattggacca tattttgatg gagagacagt
    65101 attggatctg tttgcaggaa gcggtggttt agctatcgaa gcagtatcta gaggctgcag
    65161 tcatgcggta tgcgtagaca aaaattacca agcgctaaaa atcatcaaag aaaacatcga
    65221 aattacgaaa gaaccagaga aatttaccac aataaaactt gatgcggaca aagcaatcga
    65281 acggctagca gcggagaaac aacaattcga ctacttgttt cttgatccgc catatgcaaa
    65341 acaaaaaata atcgatcaaa tccataaaat ggaagaactg ggactttttt ctaaaaccgc
    65401 agttatcgta tgcgaaacgg ataaaacagt cgaattgcca gaaactgtgg ctggattcca
    65461 tcaaataaga aaacagattt atggcataac ggccgtaact atctatagaa aagagggaaa
    65521 tcaatgaaac gtgcattatt cccaggaagt tttgatcctt ttacaaaagg tcacttagat
    65581 acggtggaac gtgcggctaa attattcgat gaggtagtaa ttggtgtttt tatcaatact
    65641 tcaaagaaaa gtttgtttcc cccagaagaa agaatgacat tgatcacaaa agctgtttct
    65701 catcttccta atgtaaaagt catgcatcaa gaaaatcaat tgacggtaga aacagcaaaa
    65761 gagattggtg cagatgcgtt gatcagaggc atacgaagta tcaaagactt tgaatatgaa
    65821 cgagagattg ctcagatgaa tcatcatcta tatgatgaat tggaaactgt cttcttatta
    65881 gcaaaaccag aatatagtca cgtgagttcc agtattttaa aagaagtact gcactttggc
    65941 ggagatgtga gcagctactt accgccagtg atcaacgaag ctttagctgg aaagcgtgaa
    66001 tcaaatgaaa gctaaacaat ggttgaaaac agtactgatt atcatatttg gaatgttgat
    66061 cattggattt gtagtagttc ccttgccgta ttatgtagaa tcaccgggag ccacgatcga
    66121 tctgaaagaa ctaattacgg tcaatgataa ggaagataaa ggttctgggt ctttctctct
    66181 tacctctgtt ggtatcaggc aagctacagg tttcttagct ttaaaagcta aaatgacccc
    66241 ttttgaagaa ttgatcagtg aagaagaatt gactggtggg gcgacaaatg aagaatataa
    66301 tcaaatgcaa cagtattata tggattcttc ccaaaatgca gcaatcgaac aagcgttaaa
    66361 acttgcagac aagccttata aaatggaatt caaaggcgta tatgtcatga gtgtagagaa
    66421 aacatcgaat ttctttggca agctttcagt tggagataca gtaacaaaag tagacgggaa
    66481 aagctttcaa agtacagaag catttatgga ttatgtaaaa agccaaaaag ttggacaaac
    66541 aatcgaaatc agttatttgc ataatggtga agaaaaaaca gcttctggtg atctgatcga
    66601 gttaccaaca gataaaaagg caggaatagg tatcacgctt actgaccata ccgagatcga
    66661 atcagataca gatgttcgta ttaattctgg ctctatcggt gggccatcag cgggattgat
    66721 gttcacttta gaaatctatg aacaagtcac tggtaaaaac ttgcgacatg gtaagcaaat
    66781 tgcaggaact ggaacgatca acagtgaagg agaagttggc cgaatcggag gaatcgataa
    66841 aaaagtggca agtgcggata aagctggcgt agagattttc tttgcaccag atgatacgat
    66901 ttcagacgat gttaagaaag aatatcctga aataaaaacc aactacgaag aagcaaaagc
    66961 tgccgctaaa aaattgaatt cctcaatgaa aatcgtacca gtaaaaacag tacaagaagc
    67021 gcttgactat ctagagaaaa tgaaatgaaa ctaaataatt aaagaggggt tgtgacacga
    67081 gttctgtcac aacccctctt tttcgtcaaa aatcttcacg ataaaaataa aaacaaaaga
    67141 aaaaattgac gtaattagat gttgaaggga cgattttgcc agagctcact gtttcttcaa
    67201 ttttaaatga aaggagtaat gaatgatttt tactggtcat tctttgaaaa aagaacaatg
    67261 atagaaaaaa tcagcaagaa aatactagct tcacccaaat tgatcatttg gatgtctatg
    67321 atagttctta tcttttgtat cgcaagtggt tttttattat caaatttgct attttataga
    67381 gaaacagaag agactgctgc aaaagaaata ctttcttcgt ttagttccag tggagaagat
    67441 ccatcagctg aaggaaacga tgtagaagag gatgctcgtc caaaggtcat gtacaccgat
    67501 atcaaaggaa gtgtgaagga gcctggtatc tattcctttt cttcagaaga acgggtctat
    67561 gatgtactaa agcgtgcagg tggattatta gaagaagcgg atagcgatcg aatcaacttt
    67621 tctgcgaaga tagaagatca gcaagtccta tatatccctg ctgtaggtga agagctgcca
    67681 gaacatcttg atcagttggc atcctcggaa ggaaaacagc ctacagcaga ttccgagcct
    67741 cccaaaataa atatcaatac agccactcca tcagaacttc agcagattcc tgggatcgga
    67801 tcagtcaaag cgcaagaaat cattcgattt agagaagaaa acggatcctt ccagaaagtg
    67861 gaagacctgc aggagatttc tggtatcggt gaaaagacag ttgaaaaact aaaaaacttc
    67921 gttacaatta aatagaatga ttcaaaatcg actggcacga gcaataaatg gacgctaatg
    67981 gatactaaga ataatccaat tctaagagct tggcaggacg aggaggtagt atttttggat
    68041 aggaaatagc ttgattgttc gcaaagaaac attaaaaaaa ccaatgctga atgtctatct
    68101 ccaaaataaa atatctggga ttcatattat gaatacggct gtcagcggca ataacagtca
    68161 agctctacga gaacgctttg ccaaagatgt acttagctat acagcagata aagtatttat
    68221 tctcattggg acgaatgatc tagcagagca taaacaactt tctaaagaaa cgtaccaaaa
    68281 aatctgcagt ggatgatagt tcagctgaaa aaagactata gttctgatca tgctttcttt
    68341 atcacgccac cagcagtcga tgagacaaaa caaaaatatc ggaataacaa gttgatcaaa
    68401 gaatatatct ctgttctcac agaagtagca aaacgccatg attgtgaagt atcgattttt
    68461 atacggaatt tcttgaacaa atggattcga agaattttta taatgggcta atggatgacg
    68521 gacttcattt cggcagtgaa gggtaggatc ttttgtcaga caggatgatt cgttatattt
    68581 aaagaatatt ttgtttaagc aatgagtact ttttaatcaa cttgaaaaaa cggaggaata
    68641 taaaacatgt tagaaaaatc ttcaaaagca tgcgatacga ataaagaaaa agttactgat
    68701 aagtcacata atgaacgcat tccttgggac cagtacttta tggctcaggc agtactgtta
    68761 tctttacgta gtacgtgtac acgtttagaa gtaggagcga cactcgttcg agaaaaaagg
    68821 atcattgctg gcggctataa tggagcagtc agtggagatg tccactgcat agatgaaggg
    68881 tgttacgtcg tggatggaca ctgcttgcga acgatccatg cagagatgaa tgcactccta
    68941 cagtgtgcaa aacttggtat cccgacggaa ggttcagaaa tctatgtcac acattttcca
    69001 tgtttggcct gtacaaaggc tttgttgcaa gctggtgtga aagggatcaa ttatctccat
    69061 gactatcgaa atgatcctta tgcccaagcg ttgatcgaac aattaggtgt atctgtacac
    69121 caagttgctt tagactcaca gcatttttca aaacttcaaa atgaattaac aggaaaacat
    69181 gtctagcgtt ttagaaaggc atgttcgaaa tcggttgatt tttccggcaa ttacgtcgat
    69241 gattatctgt ttgttgtatt acactgacaa ttggggttat cgtttgcttc ttgccggaat
    69301 ttttttatgg ttttcgctaa aagaaggacg ttttatcgcg tacttgtgta ttttgtgtag
    69361 cataacggta tccatttctt ataggctaca agagtattca tttttagagg aaaaaacaac
    69421 atctgcgcaa attactgtat ttacagatac gattcgtgca aatggtaatt ttttgacaat
    69481 ggaaggaagg ttatcagaga ataaacaaaa gctccagctt tcttatcaag taaacacaga
    69541 agcagaattg aaaacatggc ttaacttccg aggaaaggga atagaattga ctgctgaagg
    69601 cgagttcatc tctccagcaa aaaagcggaa tctttataat tttgaccaag aagcttactt
    69661 caggaaccat agaatttctg gacggttcca aattcaaaag attgaacaga tcaccatgca
    69721 aaatcactgg cgaaactggc ttcagagaaa aagaggatct tttatctcct ttgtccaatc
    69781 tgaatttccg caaaaaacaa gtgtatatat taattcttta ttatggggct ataaagatgc
    69841 aggattcaga gaaccagaag aaatttttag ggagagtggt ttgctgcatc tgttcagtct
    69901 atcgggtcta cacatccaat tttatttagg gtggctgtat tatttatttc gacgtatcgg
    69961 atggccacta cacgtcagta ttattccgtt gagtctacta actatctgtt atgtttctat
    70021 tgcaggaagt tctatttctg ttttgcgtgc tagtttgctg tttttattgc gattgctagt
    70081 caaattgctg aataagcatt attccaccat ggacttattt gcagttgtct tttggcttct
    70141 tttactattt gatcctcgat ttctttttca tgcgggtgga cagcttagtt ttttcatgag
    70201 ttttttgttt atcctgattt cttttgatgg agattggttg aaaaaaacgg cctctcacgt
    70261 cctgtttact atgatcacga tgccgttgat tgtatggcat ttttatgagt ggcctatttt
    70321 aggtagtctt tttacattac tgattgcacc ggtattcaag tttctttttc ttcctggtgt
    70381 gttatttctc acactcttca atcgctggct gccggaaact ttacttttgc tgatagaaga
    70441 gggattaatc ttatttgagc gaataatcga tttttcttca gggtcgtctc tcattatagg
    70501 acgtttgccc cttgtgctat ctgggatgta tattttcgga ttactgattt gtatggatag
    70561 gttgaaagaa caacctgtga aacacttgtt ttatgttggt cttttttctt ttattttttt
    70621 gactttgcca tttttacgct tttcttcaac gatcgctttt atcgatgtcg gtcaaggaga
    70681 cagtatcttt ttgcaaagtc cttttcgaaa agaaacgatt ttgatcgata ctggaggtca
    70741 gttacacttt gaaagagaaa agtgggcgca aggtatgata cgtcctcctg ctgaaagaaa
    70801 tatcgttcca tatctaaaag gtcaggggat aagtgtaatc gataaactca tcttgacaca
    70861 cgacgatacg gatcatgtgg gagaattacc cacgttatct cagcatttta ctatcaaaaa
    70921 aatttatatc ggttggggcg ctggacgaaa tgagccatta caaaggcatt tatctgaagc
    70981 tcaaaaaaat ggtactgaga tcattgaaat caaacatggg gacaggatca gcggttatta
    71041 tgatctttat gtattgactc cttttgaaaa gggagaggga agaaatgaag attcgattgg
    71101 tctatggatg gaatataaca ataggcgctt tttattttta ggagatttga atcaagcaat
    71161 ggaaaaacaa ttgcttttga tttatcctaa tctgaaagcc gatgtagtca aattaggtca
    71221 tcacggtagc cgtacgtcaa gtaacacaga ttttctttcc catattgaag caaaacatgg
    71281 gattatatcc tgcggcgtaa ataatcgtta tggtcacccg cacagcgaag tattggagac
    71341 actagaagaa aatcagataa aaacatggcg aacggatcaa caaggaatga tcacctatcg
    71401 ctggcatcca gtttttcatc ctcatgggct agtggaaaca atgatagact agtaattctc
    71461 atctgctcat ggtaggataa tttttgaaag tggtgagtaa tttgagtata caagcagagt
    71521 tacaaaaaat acgtaaagaa aaacttgcat cttgttactt gattttagga acagaaaagt
    71581 ttctccaaga tcaagttcgg acagaaattg tcaaaaaaat ccaattagag ggcgaagatg
    71641 acttaaattt tttaagtttt gacatggaaa atgcaagttt agatgaggta gtagctgaag
    71701 cagagacgtt gccgtttttc ggagatcaac ggttagtatt cgtagagaat ccttattttt
    71761 taacaggtga aaaagtaaac aatggaatcg aacaaaatac tgatttattg acggcttatt
    71821 tgaaagaacc attagaatcg actgtattag tattctttgc gccatatgaa aagttggatg
    71881 aacggaaaaa agtaacgaaa caattgaaaa aaacagcaat tatcatcaat gtccaacagt
    71941 tgaatgaaaa agaagtacgt caatatctga tgaatacctt agagaattcc tcaatcaaaa
    72001 tggaacgaag tgcaattgat ttattcttga ggttgaccga cttagattta tccaaaatga
    72061 tgagagagtt acagaaattg gtcctttatg gacaaaatca acagcaaatc acggtaagag
    72121 aagtagaaga attagttcct aaaacgttag aacataatat ttttgacatg acccaatata
    72181 ttttaaaagg gaagacggaa caagctttgc gtttatacga agatttagtt atgcaagggg
    72241 aagaaacaat caaaattaat gcgattttgc tttctcaatt acgtttattt ttacagacaa
    72301 aatttcttgt aaagattggt tatcagcagg caaatattgc tgaaacattg aaaatccatc
    72361 catatcgtgt aaaacttgcc atgcaggaag taaaaaaatt cgatgaacat ttgctggtac
    72421 agttgtttga tcaattagtt gagatggatt atcaaatcaa aacgggacaa atcgaaaaag
    72481 aattaagttt tcaactattc gttttgcgca cagggcagtt gttaagaaat cgataagaag
    72541 aaagacgagg gcaaagcatg cgggaaggtc gtttgttttt gtcctttttt tgacaaataa
    72601 aaaaatcggc cgaagccgat tcttttattt agcgattttt ttgcttaaac gagatttgtc
    72661 gcggttagct ttgtttttgt ggattagacc ttttgattca gccatatcaa tagctttaac
    72721 agcttcttta tataacgcat ctacgttgtc agcacctgaa gctacagcat cttcaaattt
    72781 tttgattgca gtacgcatag cgcttgtttg agatgaattt ttcacattag cgttttcgct
    72841 tgtacgaaca cgtttgattg cagattcaat gtttggcatt agtttcacct ccgataactt
    72901 aagttcttac gcgtgttacc gcataattca acattcatta ttatacctaa aggcagcgtg
    72961 ctttgcaata tgaacatgac aagttttttt ccttaacagt cttttattag gatttttttt
    73021 cagtaaaaac ggatagtaca agctgaaaaa aatcaaaaag acgctattgt gggaggcaat
    73081 agcgtcaaac caaactagat ttttatttat ctatttaaac aaagtttgaa tgggaatgtt
    73141 tggtactaag ttattatata acaaagagaa ccgtttgcaa agtttttttt gaaaagggat
    73201 acgattccct gttgttcttt gtttttctat taagaaactg cagttatttt ataaccagta
    73261 tcctttgatc agtaaaggct attctggtgt accgattatt ttaatgtatt catcgatatt
    73321 ttttatcaac atttttttta tgaccaactg gttccgacgg ttccatttgc cacttacttc
    73381 cagcacatat ttatttaggg gcaaacgtaa gatatatttt gccaaatagc gatctttaag
    73441 caggccattg atcacacggt cctcaagctg caagttgatc ccagtcagca tcattggctg
    73501 ttcttgtaaa acgtacgtat caattaaaag accttcaaaa gtttccacaa gcgtcttcct
    73561 ttcttttcaa agtaacctga aaacaggcac taattcttcc attttgatat tcgttgaagt
    73621 ggatgcgatc attctttctt ttgtccactg taatctagta taacaaaaaa gagcagaaga
    73681 gccaaaaaga aaaaagagca tcgaattcta taggaagtct cattggcacg gcctaaatta
    73741 ttacaaaaca ttgattaaaa aaagcccctg catcgcagga gcatccccta tatcgtttta
    73801 ataggtcaat caaaaagcag tatcaaacac attttgtatc aaatcaggaa ataatagata
    73861 agctaagccc agaatcaaaa gtaccgctag aagaaaccag ccgattgttc ctatgaaata
    73921 ctttaatttt tcgaacattg tatcacctcg ttgtcagtat atcacggatc agtaaattgg
    73981 gggtgttaag aatctcctaa aatagacaaa atttgttcta ttttcctaaa gcaaatactt
    74041 gtaagataca gtaagtgata ccagaagcaa tgaccggtcc ggcggcgatt cctcttaaga
    74101 agacaacacc taaaatcgtg ccgaaaacaa gtgcaacagt gatttgtgga tcagaagcaa
    74161 tcaatcctac acccttggaa gataagacag ccactagccc accgcaaaaa acagctaccc
    74221 agccaatagg ggatttaaag gcttctaata gatcacgtag accgatttct cctgttgcga
    74281 tagggattag aatagccgct gtgatgatcg tcaccccaat attgattcct ttgccttgaa
    74341 gccaagggaa aaaggaacgg gaggctggta tgagcttcat aatcaaaaca aaagctgccg
    74401 ctatcatcag actttgattt ttagctaaaa ttgctacaag taaaataatt cctaaaaaaa
    74461 gccaactttc catgaaatat attcctcact ttcttcgaaa caatcataac atgtttttag
    74521 aaagttacaa aagtaggaat tcatccttca agacctattc ataagctgtt tttttcataa
    74581 aataaacaaa atcataaaaa aacactttgc ttatttgaca gaagacagcc gcgctttgta
    74641 tagtggacaa gacttacatt tttttgaaga atcagaaaga tcaggtaaag gtcttagtaa
    74701 acgcaagcca ttcagaataa ccaatattgt gcttccttcg tgtcctacaa ctccaagagg
    74761 tagatttaaa aattgcagga agttagaagc aatcaacaag agaataacaa gaatcgaaaa
    74821 aataatattt tgtttgatga tccgccgcat ttttaaagat agccgatgac tgtagacaaa
    74881 tttatttaag tcgcttttca ttaagacgat gtctgcgata tccatagcgg catctgtccc
    74941 ttgccccata gcgacaccta aagaagcggc tgctaaagca ggcgcatcat tgactccatc
    75001 accaaccatc gcatttactt ggtaaagttc attctctttt ttgactagtt gtgttttttc
    75061 ttccggagta cagcttgcat agtaatgttc aatccctact tgttcagcaa tggcctttgc
    75121 tgtgccagaa tggtcgccgg ttatcatact tgtatggatg ttttgggatt taaagtagga
    75181 aattacttgt tgggtatttg cttttggaat atctagcaga cctaaaacag caattaactg
    75241 ctgatcttta gaaagataaa tcacggtttt gccttgattt tccaaacgtt cgatcatttc
    75301 cgcaatctct ttgctgatta tcatcgtctc aggatcatat gcgtgcttgc caactttcca
    75361 ttgcgcacct aaataggtag tttgtaatcc aaaaccagta atttcttcta ttggtaagta
    75421 ttggatttct tcaggtattg tgcaatcgaa atattgcaaa atcgcatgag caagtggatg
    75481 cgtcgttttt tgttccatag caacaagaag ttgttgtgcc aaatcctttt catcaaagaa
    75541 aaagctgtca gttacaactg gttttccttt agtgagcgtt cctgttttat caaaggcaat
    75601 cgcttttaat tcagaaagct tttctaaatg gataccgcct ttgattaaaa tcccattttt
    75661 tgcagaattt gaaagagcag ctagagaagc tggtgtagca gatgcgacaa gcgcacaagg
    75721 agaagccaca actaataata ccattcctcg ataaaagctt tcatcccaac tccaacctag
    75781 gaaaaagtgg gggagaagga tcattagagg aacagcaagc aaaatcaatt ttacataatt
    75841 attttcgatt cgttcgataa atgaagctgt ttgagttggc gtattctgcg cttcttctac
    75901 taattggata atcttagcaa agacagtttc ggaactcgtt tttgttactc gaatcgtgat
    75961 gggatttcct tgattcagcg ttcctgcata catctcatcg cctaaatatt tttctacagg
    76021 cacagattca ccgcttaatg cagactcatc gatcgttgcc tgtgtgcttt ctaaaaagcc
    76081 atctatcgga acagctgctc ctttagcgac aaataccaag ttatccacct ttagttgaga
    76141 aacatctact tcttgcattt gtccattttc ttttaataat tgggcttttt gtggttgaag
    76201 attcatcaaa gcagtaattt ctttttgact tttattagtt gtgtattcct ctaatgcacc
    76261 gcttaaacaa aagatgaatg tcaacattgc tccttcaaag taattaccga tcaaacaggc
    76321 accaatagca gctaaagcca tcaataaatc cacattcagt gtccgatcat tccacaattc
    76381 tttcaagcct tccgatgttt gtttatatcc accgatgata atggcactga tataaaaaag
    76441 tggggtgatg gtaagagaga atcggtcgaa gataaaaccg aagatcataa ataaaaggca
    76501 gccgattgtt gctgcaaaag cttttttctg attagtcatc gttattagac ctccttgtca
    76561 aaacaaggat agcataataa aaatgtaatt gataataata acagctaaat gagaacgata
    76621 atggttatca tttggtgatt aagcttgatg ttgtttttat tatttataat gattattatt
    76681 aaagaaaata aaaaaccaga acatttctca aataagagaa gtgttctggt tttttctaca
    76741 ttgcaataaa ccaataacca atcaaaatga taccaaggat aatacgatac cagccaaaag
    76801 cggtaaagtc gtttttcttc aaatagctta gtaagaattt gatagctaag acggaaacca
    76861 caaaagatac gaatgttcct acaagaagaa caacagtttc ttccattttg aatgaattcc
    76921 cttcagctaa atatttgacg atcttcaagc cgcttgcgcc aaacatgaca ggaatcccta
    76981 agaaaaatga aaactctgta gcgacaaaac gcgaagcacc aatcaagata gatcctagga
    77041 tcgttgcgcc agaacgggat gttccgggaa caagtgccaa gacttggaat aacccaacaa
    77101 gaagagctgc ttggtacgta aagtcattca gagaagtcca acgaggtgtt ttgtttttgt
    77161 tgcgtttttc gacaataata aaggcaatac cgtaaacgat caaaacaatg gcaactggta
    77221 agaacttgtg gaaatttgct tctaaccaat catccaaagg caaaccaatg atgatggctg
    77281 gtatgcaagc aacgatcact ttaaaccaaa gtgtccacgt atcttttttt tctaccgttg
    77341 atttacttgg agaaaatggg tttaacttgt gaaaataaag aacgacgaca gccaagatcg
    77401 cccctaattg gataacgaca ttgaacatcg acataaattg tgaactggca tccagtttga
    77461 tgaattcatc tgctaggatc aaatgaccag tactactgat gggcacccat tccgttattc
    77521 cttcaataat accgagaatc actgctttga taatatttgc aaaaaacata aattccttcc
    77581 ttcccaatta agatacatct tatagtatac cgttattttt tgagaaaccc aatctgtttt
    77641 ttaatattaa gaaaaacttg agaaataaag cagataagta cacgaagaga aaaaagaaga
    77701 caaagccctc gttcagactt tgccttttat gattctctgg taaaaattaa aatatcccca
    77761 ttatagaaac gagtcgcgac tatgtgtttt cccgttatcg atataagacg tatcattgat
    77821 gccgtggata gaaaatcgac cgtttgcata agtgaccttt gttacgctgc catttaaaag
    77881 cccagcacga acaggctgtt taggatcgat cagattaagc aaaaaggcga tcgtcaaacc
    77941 atgagatacg actaaaacat ttccaccacc atttttttcg cagtgatggg cgatatcttc
    78001 aaatcctgtc catacacggt ctcgcaatgt ttcataagtt tccgcccacc cagcggtatc
    78061 taagttgtat acagcattag caatttgttc aaaagaaact tctgtggtaa acatttcatc
    78121 atatgtttta aaattcaata cacgagggat cacaccccac atttccatgt catatccacc
    78181 ttcaagagac ccgaaacacc attctctaat ccttttttca ataaaataag gaatttcttt
    78241 tcccttagaa tgttctccta gaataatacg ggcagtttcg attgcacgac cactgtcact
    78301 agaataagca aatgaaaagt cgacattttt taacccgcga cctaaataat gaatgccttc
    78361 ttgtccttgc tttgtgaggg gtgtatcaca ccagccttgt actcgttcaa ttgtattgaa
    78421 catcgttttt ccgtgtcgta cgatataaag tgtagtttct gtcattttat tctcctccta
    78481 agtcgtttta acgattgaaa aaaggatttg tccttttttc ctgttcaatc gtcgttgcat
    78541 cgccatgtcc tggatagact gcaaattcat caggtaatac aaacagctgg gtccgaatac
    78601 tatgaagtaa ttgttctaaa ttccctgtat acagatcggt tctaccgata cttcctctaa
    78661 acagggcgtc gccagaaacg acaaaatcat caaaaataaa gctgacgctt cctatagaat
    78721 gccctggggt agggacgacg cgaaaagaca tgtttcctaa agtataagtt ttcatttcaa
    78781 attcatattc tgcaggcgag acaataacat cagcaatatc atcatgtcta ccgagtccag
    78841 aaagattcaa aatcggatca cctagccatt tttgctctag tgggctgaca taaactggga
    78901 tttgataata cgcgcgtaaa ggttccactg caccgatatg atcgtagtga gtatgggtaa
    78961 gcaaaatcgc taccggctgt tggtttgttt ttttgatttc ttgtttgatt cgttccgcat
    79021 cttctccagg gtcaatgatc aaaagtgctt cttcgttgta gaccaaataa cagttttctt
    79081 caatcattcc tgtttttaat ctttcgatat gcatgtgcat tcctccttga tagccaaaac
    79141 ttctttcatc cattagtata acgcattcat tgctaggaaa caaaaacagg tacttatttc
    79201 tctttactta tctgaattat cagactatat gaaaagtatt tggatattct aaagagaagg
    79261 aaagtaagtt ttcatagcat tttaatatgt ccttgttaca ttagattcat ccaaaaatcc
    79321 aaattctata tcctatatcc ctgcctaatc agcagggtgt ttttttatgt ttggaaagag
    79381 agctcttttt gctaaactag gttttttcag gtaatcttag agagaaagag gtggtggaaa
    79441 tgatcaaaca gtcaaaaata tctgtgcggc acttagtaga atttattttg cgacgtggaa
    79501 gtatcgacaa tcgtaaaaaa agtaaccata ctgcattaga aggagcgaga atccaccgca
    79561 agctccaaaa agatgcggga gaaaactatg aaaaagaagt ctttttaaaa acaacagttc
    79621 aattagatga atattacctg atcgttgaag gccgtgcaga cggtattttc aaaagagacg
    79681 gtatttatta tatagatgaa atcaaaacct ccgaaccacg atttgaagat ttagaaccag
    79741 aacaagtgga acttttcttt catcaagcaa gggtgtatgc ctatatctat agtcaagaac
    79801 atgatttaca agaaataaat ctgcaattga cttacttcca gacggcagaa gaagtgatca
    79861 cgagaaaggt tgaacatcag acaagagagc agttagaaac tttttttaaa aagctgactg
    79921 aagattataa agaatggctt gtttttcaag aaaactggcg cactgtacgc aatgcctctt
    79981 tgatggcttt gaaattcccc catgatgagt ataggaaagg tcagcgtgaa ctagcggtag
    80041 ctgcttataa aacgatccgt acgaaacaga aattgtttgt agaagcacct accggaacag
    80101 gaaaaacaat ctctacgtta ttccccgctt tgaaagctgt aggggaagaa gagggagaaa
    80161 agatctttta tctaacggct aaaacaataa ctagacaagt agctgaagat gctatgacag
    80221 cattaaaaga tacaggagct gaagtaaaaa gtgtcacttt gacggcgaaa gacaagatct
    80281 gctttttaga tgagacgacc tgtaatccag atcagtgccc atatgccaat ggttattaca
    80341 atcggattaa tgaaggttta tgggatcttt tgaatcatga gaatcagatc actcgtgaag
    80401 tcatcgaaac ttatgcaaga aagcatacac tttgtccttt cgaactatca ttagatgtca
    80461 gtatctggtg tgacgtaatc attggggatt acaattattt atttgatcca actgtttatt
    80521 taagacgttt ttttgaagat gaaaaaaacg aggactatct gtttttgatt gacgaagcac
    80581 ataatttggt gaatcgttcc cgagagatgt attcggcaga actatcttat gaaaaaacga
    80641 aacgaagtaa ggaagctata ccaaaggaat tcaaaaaact tcatcgccgt ttcaacaaac
    80701 tattaaaaga atttgattcg atccgagaaa tagcaaaaga agatcattgg gattatcatc
    80761 atcaaaaagc accggctgaa tctttagtaa aggcaggtta tcaattaagc gaaaaaatta
    80821 aagaatggct ggcagaattt cctgagcacc catcacaaga acaattgctt ccttattatt
    80881 ttgatttgct tcattttttg aaggtcagtg aattttatga tgatcactac gaaacaacag
    80941 tcgaaaaaac gtatcgcgat ctgatagtaa aagagttttg tattgatcca agcttgtttc
    81001 ttgagcagtc gttggataaa ggaaaaagca gtattctttt ttctgctagt ttttcaccac
    81061 tctcttatta tcaggaaaca ctggggggac aaaaaagtct ggcttacaaa ctgccgagtc
    81121 ctttcccaga aggaaaccag caagttttca tcgcaaatta cctagagacg acttatcgaa
    81181 aaagggaaca cagtctggca aaattagtag agacgattca tgcttttgct gatggaaagg
    81241 tcgggaatta tttcgttttc tttccttctt atcaatattt agatcttgcg gtggaagcat
    81301 tcagacagca atatcccgac agcaatgtgc taatccaaga aacggacatg aatgaggaag
    81361 aacgcgaacg atttttaaca aaatttcaaa caaatcccgc tgaaacattg attggatttt
    81421 gcgtacttgg cgggattttt tcagaaggaa tcgatttgag aggggaccga ttgatcggca
    81481 gtatgatcgt aggtgtaggt ttgccgcaga tgaatcatga gcaggaactg atcaaatcat
    81541 attatgatga gaaggaacac cttggatttg cttacgctta tcagcttcct ggaatgaaca
    81601 aagtcttgca agctggtggt cgagtgattc gtggcatggc ggatcaagga atcgttgtat
    81661 tggcagatca acgattttct agtttttctt atcgacgttt attcccacaa cattggcaaa
    81721 cagcaagaga agtacgaagt gttggggaat tggaagaagg aatacagcgt ttttggttat
    81781 caaaagaagc agtaaatcaa aaaaacgagg aatgacagac gtcagcctcg ttttttgatt
    81841 aacgatagtt agtgaattgc aactcgattg gtaagtcaag tttacgtaat aaagcaatca
    81901 cagcttgtag atcatcacgg ttcttacctg tcactctaat ctgatcttct tggatttgtg
    81961 cttttacttt cagtttttca tttttgatag ctgtagtgat tttcttcgcg ttttcacgat
    82021 cgattccgtt gaccaaatcg gcagtttgtc gagctttgcc acctagagcg tgttctgatt
    82081 cggaaaaatg gatatttttg attggcacat ttcttttgat cagcttacca aataatacat
    82141 ctttgatctg ttcgattttg aaatcatcat cgctcaatac aactagattg ctgttctcta
    82201 atttgatttc tactgtagaa tccttgaagt caaagcgatt ggacaattct tttgttgttt
    82261 gctgaatcgc attttttact tcttctgtat tgacttctga tacaatgtcg aagctagcat
    82321 cttttgccat aggaaccctc ctgatataca tataaatatt taccatacat ctatcttatc
    82381 ataggattag ggcagattct tacctgtttg ctaaaattct tctcatttga gttcgaagta
    82441 ttttatttta atagataaac tgttattctt acatttggaa taccaaagta ggggagaggt
    82501 gcttgtgagt aaaattatga ctgcgtatca taaaaatgaa agattaaaga aaatagtggt
    82561 tattttgata accggattga ccgctgcggt cgctttaaat tattttttga ttccagccaa
    82621 tgtcttttct gcagggatga atgggatcgc tcaaatcatt tcctcgcttt tatatgaatg
    82681 gtttcatatc aagatagaca caggaacatt tatttttctt ttgaatatcc ctgtgtttgt
    82741 attagggttt ctcaaagtag gaaaacgagc aacgatcctt agttttgtga atgttgtatg
    82801 tgtatcggtg atgacgatgc tgcttccagt cggacagatc actgataata ttttgatgaa
    82861 tgccctagta ggcggtgttt tattaggtct tggcgtagga ttatctttaa aaatgggctt
    82921 tacaactggt gggatggata tcatctctct tgtactttca caaacaactg gaaaaacagt
    82981 cggaaactac atgctgattc taaatggtat cattgtagtc atcgcaggct tcttctttaa
    83041 ctgggaaagt gccttgtata cgattatttc catttatgca atgactcaag taattgatgc
    83101 catccacacg agtcatcaaa aagtaacagc aatgatcgtt accgtcaagc cagaagaggt
    83161 aacagaagaa ctagcaaaac ggatggtacg tggaatgaca ttactgccgt ctgtcggtgg
    83221 ctattcaaaa aaacaaggga acatgatcat gatggtcatc acccgctacg aaatgtatga
    83281 tcttgatcag atcgttcatg aaatcgacga cgatgcattt atcaatattt taccaactca
    83341 ttcagtattt ggccggtttg caaatgaaaa cgaacaaaaa atgtatcgaa caactggcgt
    83401 attgccagaa ataaaaaatc gttcacaaaa aaaatgagga atcaggttta ttttagaata
    83461 aatttaatct attacatcta ataagaacat ttttataaat ttaaacgaga atcttacttc
    83521 taaattctca tttacaagca gctaaaaaaa gagtatgata aataaaaatc gtgttaagga
    83581 gtgtcgtacg atgagcgaag caaaccaaga acggtctgga caagaaatag aactaatcaa
    83641 agaacgtatt cttgctgcct tggaaatggt gattgatcct gaattaggaa tcgacatcgt
    83701 caatttaggc ttgatttatg atatagaatt caatcctgaa aatggagaaa cagtcattaa
    83761 gatgactcta acaactatgg gctgtccatt agcagatatt ttaacagaat ctatccatga
    83821 cgcattaaaa gaagtgccag aagtaacgaa agcagaagtc aaactagtct ggtatccagc
    83881 atggacgaca gataagatga gccgttatgc acggattgcg ttggggatac gatagaaatc
    83941 ttggctgact agctatgaga agcaaatagg aaaaacaaaa aagcctttta tcctttctag
    84001 aagaaaagag ataaaaggtt tctttgttta taaaattttg tctgctatat tataagttaa
    84061 ttaaataaat gtgttctatt gataaaggac attcaaatcc ctttcctttt ggaatccttc
    84121 ttttttacat cagtttgatt ttcgtatagt attctttctc atcttcacga taaatcgcag
    84181 ccccattttt ttgttgtgct ttaagactgc caagattttt tttatttaca gacaaaagtg
    84241 cctcatcaat gccgattttt tttgcctctt gtagtactaa tgctaatcct tgtgtggcat
    84301 atcctttttt acgataatta ggggagatgc cgtaaccaat atggccagcg ccgtttctta
    84361 atgtttcgtt cagtttatgg cgcaaattaa aaattcctac atactcatgt tcatcacttt
    84421 ctaaaataaa gacggttgcg ggaacgtaac tctcaggaag atcgattcct aaagacgttt
    84481 tttcgcattt ttttacataa tctaaaaatt cagcaaatgt atagccataa gcattgttga
    84541 taaatccact ctcatttggt ccaaagctag tttgtagctg ataagctttc tttaaatctg
    84601 cttcccatag tttgatcagt ttcatcgtat tcacctcttt aagtaacctt attcatttgt
    84661 tttctactat agcactattg ttgtaaatag ccatgcctat ttttatttat tgtgtcatat
    84721 cgtattaaga atcatcgaac aatacaaata tgaattacaa taaaaaaatt atttgaagtt
    84781 cgttcattcg ttacttatga tctctagaag aaagaaaacg tgaattactt cagcgtctag
    84841 aattccagaa ggatagtaat caacagaaga aataatcaat atccaaatga aagaagatag
    84901 ctgaatcgag aaaaagagga aaaaagaaat gaagaaaaaa ataaaatagt tactaatccc
    84961 agagtcgttc gtttaacatt gaaatcgatc atatggataa aacagatttt ttcaccaacc
    85021 ctcaatagat gatggagttg ctcggatgaa ttatttgtgt agatggcata agggagggcg
    85081 tacagaaatg aaaaagaaca aaaatatgtt cttgacaaaa aacacatgta atcctaaact
    85141 aaaagatgtg atgcggctat ggcggaattg gcagacgcgc tagcttcagg cgctagtgag
    85201 ggtgacctcg tggaagttcg agtcttctta gccgcataat ttgttttttt ataaagagaa
    85261 ggtacacctg gagcgatgag aaagaaaata acatattatc taaaagaata taggaaaaat
    85321 tttaaacaac ggaaccagat tcaaataaat caaaaaaaat ttgtgtgaag gtgggacagt
    85381 ttaaaaccta atagattgat tcaaatagta gatttataga ttctgataca agaaatactg
    85441 gcaaacgata gttaaggaat aaagactaaa cttaaactca aaaagaggtc atgcgaaaga
    85501 ataaatcgca tgacctctta ctatattttg aatgtgttta aacgttcaaa taatgaatct
    85561 tagtaccaac cgtttgcttc ccagaatgct ttcgccgctt cccaagaacc ataacgtgaa
    85621 gtaacatatt gttctgctac tctttcttgg tttgcagcag agtagtcacc atttaagtaa
    85681 gaagaatcta attggtaacg gccgatgtaa cgtccgttcg ttgctgtgta agaaccgctt
    85741 gattcttttt gtgcgatcca ttcttttgca gagcttgtac ttgctggagt agcagcagtt
    85801 tctgttacag caggagcagg ttgtgtttca gcaacaggcg ctgcttgtgt ttccgtttga
    85861 accgtttctt gtacaacagg ttgttcagct tgaacaggtt cagcttggac tggtgcttct
    85921 tgaacggctg cttgaggttc agcagctgct tgtgcagagc cttctgttgg gattgttaat
    85981 tgttgtcctg agtagatcag gttaatatca gaaattgaat ttgattctgc aatggcattg
    86041 atcaaggagt tatcaccaac gtatttttga gaaatcgttg atagagtatc acctgattga
    86101 acggtataag cttcgtccgc atgagcagtc gttcccatga agaatgcagc gccggcagcc
    86161 aaagttgttc caaaaagtaa agttttaagt gatgtcatga aaaatctcct ttattcgtta
    86221 aaaagtttta tttgttgtgt tcaacgagga atactttaac acgtgaaaat atttcatagg
    86281 taaaagaagc atgacaattt atgacaaatc cacgtttttt gtaacagaat gatgttcgct
    86341 cgatgtgaaa aatgatgagg aatttcacat aatgcagaaa taatgggatt agaacaggtg
    86401 ttctgatgag aaaagaaatg tataaataac ttgaaagaaa ttgcttttgt tcatcatttt
    86461 atttacatat tgcgtatatt ttttgtattt ttgcttgaga tgttacaata aaagaaaaag
    86521 gaggcggttc caatgagtaa aagataccag aacatcatgg tagcagttga cggatcaaga
    86581 caatcgatca aagcgttcgc tgaagccctt gacttagcaa aggacaatga agcccactta
    86641 tttatcgtgt caatcatcaa caaagttgag ctgacccata gtgcctatgc cttttcaaaa
    86701 atctatgcag atgaaaaaca aagaacagaa gtagccatgc tgaaaaaaat caatgatgcc
    86761 aaagagtacg gcattgctga gatccacgca atagttgaaa ctggagatcc aagaaatttg
    86821 attgccaatg tcatccctgg acaagaaaat attgatttga tcgttatggg agcgacagga
    86881 aaaggtgcta tacaacaagc attagttgga tcgaccgctt cttatgtcgt atcacatgcc
    86941 ccttgcagtg tgttggtcgt gaagtaatga gataagggag gaatagaaaa tggttcgtcc
    87001 tcaagaagtc cgtgcgccaa aagaaaaaat agaaatttta gcaattattg aagacggcac
    87061 acaaacaaag aagggatact cgatcgcttt agtaaagtgg aaaggaaaaa aaggtgttgc
    87121 tctgagatgg gatggagata accaacaaga taaaggattc cctgtcacag ctaacggtta
    87181 tcatccagcg tggtttgttc tgcctgataa atttatggaa ttatacagct atgattatgc
    87241 aagagccgca gcatcaatca aagcattaga taaattaaca gaagagcaga atgaaatgga
    87301 ggaaaaataa aaaagacgaa ccaatcagcc tttagaaaac tgattagttc gaggagtaaa
    87361 aatgaaaaaa ggttatttgg gatgaaacta gaataatgcg tataacttaa ataaaactta
    87421 aagcaataaa atggcaagag ggtgaggcaa atggaagcag gaaaagtatt acaggaagta
    87481 aaagcactat gtgcagataa aaaatatgaa gaagcgaaaa gcctgatcga aaagaacaaa
    87541 gaaaatttgg aagaaaagta cccgatcgca caagaattta tagaattaaa acaaggaacg
    87601 atccttcacc gattcaaaca tttttttggt ataacagaat agaaaaaacg attaaagatc
    87661 ggcagaaatt tgctgcatct ttaatcgttt tctttttaaa catgcgaggg attcaattct
    87721 gattcttata taaaaaatag atccaaataa taagaaaaat tactgaaagc gtcaattcga
    87781 tgatgtgtgt tattaataca gacgttgttt cccaatgcct aaaagaaaaa atttgataag
    87841 taaaatatac agcaggtaaa gaggccagta aaactgaaaa cttctttttt gtccatttta
    87901 tgtaatagaa aagaatgctt atcataataa aaaaaggaat ataaaaaaca aattgaccga
    87961 tcgcgatcat actattgcct ccgataaata aagtccatga tacaaaataa cactttgtgc
    88021 gaaatggcgc aagcagtttt gtggatttat ccggtaaaat ctgtatcgat tttgaaatta
    88081 gaggagaaac t
//
LOCUS       DACDIH010000004        77726 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDIH010000004 DACDIH010000000
VERSION     DACDIH010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.004"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(48..1217)
                     /locus_tag="IV281_000259"
     CDS             complement(48..1217)
                     /locus_tag="IV281_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HAP8779304.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            complement(1288..1838)
                     /locus_tag="IV281_000260"
                     /pseudo
     CDS             complement(1288..1838)
                     /locus_tag="IV281_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
     gene            complement(2041..2520)
                     /gene="rlmH"
                     /locus_tag="IV281_000261"
     CDS             complement(2041..2520)
                     /gene="rlmH"
                     /locus_tag="IV281_000261"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP8779305.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            2948..4237
                     /locus_tag="IV281_000262"
     CDS             2948..4237
                     /locus_tag="IV281_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8779306.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4276..4881)
                     /locus_tag="IV281_000263"
     CDS             complement(4276..4881)
                     /locus_tag="IV281_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP8779307.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(5086..5550)
                     /locus_tag="IV281_000264"
     CDS             complement(5086..5550)
                     /locus_tag="IV281_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8779308.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5572..5892)
                     /locus_tag="IV281_000265"
     CDS             complement(5572..5892)
                     /locus_tag="IV281_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8779309.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(6036..7115)
                     /gene="pepA"
                     /locus_tag="IV281_000266"
     CDS             complement(6036..7115)
                     /gene="pepA"
                     /locus_tag="IV281_000266"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP8779310.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7315..7665
                     /locus_tag="IV281_000267"
     CDS             7315..7665
                     /locus_tag="IV281_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8779311.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            7888..8835
                     /locus_tag="IV281_000268"
     CDS             7888..8835
                     /locus_tag="IV281_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8779312.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(8892..10310)
                     /gene="pepV"
                     /locus_tag="IV281_000269"
     CDS             complement(8892..10310)
                     /gene="pepV"
                     /locus_tag="IV281_000269"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP8779313.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10459..11299
                     /locus_tag="IV281_000270"
                     /pseudo
     CDS             10459..11299
                     /locus_tag="IV281_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
     gene            11303..11743
                     /locus_tag="IV281_000271"
     CDS             11303..11743
                     /locus_tag="IV281_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP8779314.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            11793..11987
                     /locus_tag="IV281_000272"
     CDS             11793..11987
                     /locus_tag="IV281_000272"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779315.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12123..12809
                     /locus_tag="IV281_000273"
     CDS             12123..12809
                     /locus_tag="IV281_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8779316.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13217..13906)
                     /locus_tag="IV281_000274"
     CDS             complement(13217..13906)
                     /locus_tag="IV281_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779317.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(13881..16010)
                     /locus_tag="IV281_000275"
     CDS             complement(13881..16010)
                     /locus_tag="IV281_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP8779318.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(16058..17395)
                     /locus_tag="IV281_000276"
     CDS             complement(16058..17395)
                     /locus_tag="IV281_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP8779319.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(17647..18006)
                     /gene="rplT"
                     /locus_tag="IV281_000277"
     CDS             complement(17647..18006)
                     /gene="rplT"
                     /locus_tag="IV281_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP8779320.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(18059..18259)
                     /gene="rpmI"
                     /locus_tag="IV281_000278"
     CDS             complement(18059..18259)
                     /gene="rpmI"
                     /locus_tag="IV281_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP8779321.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(18331..18855)
                     /gene="infC"
                     /locus_tag="IV281_000279"
     CDS             complement(18331..18855)
                     /gene="infC"
                     /locus_tag="IV281_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP8779322.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            19175..19807
                     /locus_tag="IV281_000280"
     CDS             19175..19807
                     /locus_tag="IV281_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8779323.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(19809..20714)
                     /locus_tag="IV281_000281"
     CDS             complement(19809..20714)
                     /locus_tag="IV281_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8779324.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(20799..21452)
                     /locus_tag="IV281_000282"
     CDS             complement(20799..21452)
                     /locus_tag="IV281_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8779325.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            21774..22718
                     /gene="mvk"
                     /locus_tag="IV281_000283"
     CDS             21774..22718
                     /gene="mvk"
                     /locus_tag="IV281_000283"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP8779326.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            22711..23688
                     /gene="mvaD"
                     /locus_tag="IV281_000284"
     CDS             22711..23688
                     /gene="mvaD"
                     /locus_tag="IV281_000284"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP8779327.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNFKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            23701..24786
                     /locus_tag="IV281_000285"
     CDS             23701..24786
                     /locus_tag="IV281_000285"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP8779328.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            24783..25847
                     /locus_tag="IV281_000286"
     CDS             24783..25847
                     /locus_tag="IV281_000286"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP8779329.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26096..26899)
                     /locus_tag="IV281_000287"
     CDS             complement(26096..26899)
                     /locus_tag="IV281_000287"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP8779330.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(26919..27761)
                     /gene="kduI"
                     /locus_tag="IV281_000288"
     CDS             complement(26919..27761)
                     /gene="kduI"
                     /locus_tag="IV281_000288"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP8779331.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(27777..28784)
                     /locus_tag="IV281_000289"
     CDS             complement(27777..28784)
                     /locus_tag="IV281_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP8779332.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(28791..29435)
                     /locus_tag="IV281_000290"
     CDS             complement(28791..29435)
                     /locus_tag="IV281_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8779333.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(29535..30293)
                     /locus_tag="IV281_000291"
     CDS             complement(29535..30293)
                     /locus_tag="IV281_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP8779334.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30420..31571)
                     /gene="fucO"
                     /locus_tag="IV281_000292"
     CDS             complement(30420..31571)
                     /gene="fucO"
                     /locus_tag="IV281_000292"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP8779335.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(31653..31964)
                     /gene="rhaM"
                     /locus_tag="IV281_000293"
     CDS             complement(31653..31964)
                     /gene="rhaM"
                     /locus_tag="IV281_000293"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP8779336.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(31966..32811)
                     /gene="rhaD"
                     /locus_tag="IV281_000294"
     CDS             complement(31966..32811)
                     /gene="rhaD"
                     /locus_tag="IV281_000294"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP8779337.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(32825..34081)
                     /gene="rhaA"
                     /locus_tag="IV281_000295"
     CDS             complement(32825..34081)
                     /gene="rhaA"
                     /locus_tag="IV281_000295"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP8779338.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(34078..35538)
                     /gene="rhaB"
                     /locus_tag="IV281_000296"
     CDS             complement(34078..35538)
                     /gene="rhaB"
                     /locus_tag="IV281_000296"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP8779339.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            35691..36560
                     /locus_tag="IV281_000297"
     CDS             35691..36560
                     /locus_tag="IV281_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8779340.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(36686..37585)
                     /locus_tag="IV281_000298"
     CDS             complement(36686..37585)
                     /locus_tag="IV281_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8779341.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(37750..38574)
                     /locus_tag="IV281_000299"
     CDS             complement(37750..38574)
                     /locus_tag="IV281_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8779342.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(38585..39880)
                     /locus_tag="IV281_000300"
     CDS             complement(38585..39880)
                     /locus_tag="IV281_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP8779343.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(39896..40375)
                     /locus_tag="IV281_000301"
     CDS             complement(39896..40375)
                     /locus_tag="IV281_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP8779344.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(40381..41409)
                     /locus_tag="IV281_000302"
     CDS             complement(40381..41409)
                     /locus_tag="IV281_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP8779345.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(41426..42532)
                     /locus_tag="IV281_000303"
     CDS             complement(41426..42532)
                     /locus_tag="IV281_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8779346.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(42761..45358)
                     /gene="adhE"
                     /locus_tag="IV281_000304"
                     /gene_synonym="adhC"
     CDS             complement(42761..45358)
                     /gene="adhE"
                     /locus_tag="IV281_000304"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP8779347.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(45663..46043)
                     /gene="yajC"
                     /locus_tag="IV281_000305"
     CDS             complement(45663..46043)
                     /gene="yajC"
                     /locus_tag="IV281_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP8779348.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(46140..47285)
                     /gene="tgt"
                     /locus_tag="IV281_000306"
     CDS             complement(46140..47285)
                     /gene="tgt"
                     /locus_tag="IV281_000306"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP8779349.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            47503..48201
                     /locus_tag="IV281_000307"
     CDS             47503..48201
                     /locus_tag="IV281_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8779350.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(48369..51008)
                     /locus_tag="IV281_000308"
     CDS             complement(48369..51008)
                     /locus_tag="IV281_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8779351.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(51627..52658)
                     /gene="queA"
                     /locus_tag="IV281_000309"
     CDS             complement(51627..52658)
                     /gene="queA"
                     /locus_tag="IV281_000309"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP8779352.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            52851..54026
                     /locus_tag="IV281_000310"
     CDS             52851..54026
                     /locus_tag="IV281_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8779353.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            54030..54671
                     /locus_tag="IV281_000311"
     CDS             54030..54671
                     /locus_tag="IV281_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8779354.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            54668..55585
                     /locus_tag="IV281_000312"
     CDS             54668..55585
                     /locus_tag="IV281_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8779355.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            55582..56253
                     /locus_tag="IV281_000313"
     CDS             55582..56253
                     /locus_tag="IV281_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8779356.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(56794..57282)
                     /locus_tag="IV281_000314"
     CDS             complement(56794..57282)
                     /locus_tag="IV281_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP8779357.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(57483..57749)
                     /locus_tag="IV281_000315"
     CDS             complement(57483..57749)
                     /locus_tag="IV281_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8779358.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(57749..58012)
                     /locus_tag="IV281_000316"
     CDS             complement(57749..58012)
                     /locus_tag="IV281_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8779359.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(58005..58277)
                     /locus_tag="IV281_000317"
     CDS             complement(58005..58277)
                     /locus_tag="IV281_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779360.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            58488..58673
                     /locus_tag="IV281_000318"
     CDS             58488..58673
                     /locus_tag="IV281_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8779361.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(58815..60638)
                     /locus_tag="IV281_000319"
     CDS             complement(58815..60638)
                     /locus_tag="IV281_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP8779362.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(61236..63941)
                     /locus_tag="IV281_000320"
     CDS             complement(61236..63941)
                     /locus_tag="IV281_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP8779363.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(64247..65194)
                     /locus_tag="IV281_000321"
     CDS             complement(64247..65194)
                     /locus_tag="IV281_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP8779364.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            65384..65653
                     /gene="rpsN"
                     /locus_tag="IV281_000322"
     CDS             65384..65653
                     /gene="rpsN"
                     /locus_tag="IV281_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8779365.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(65829..66191)
                     /locus_tag="IV281_000323"
     CDS             complement(65829..66191)
                     /locus_tag="IV281_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8779366.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(66202..67323)
                     /locus_tag="IV281_000324"
     CDS             complement(66202..67323)
                     /locus_tag="IV281_000324"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP8779367.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(67339..67689)
                     /locus_tag="IV281_000325"
     CDS             complement(67339..67689)
                     /locus_tag="IV281_000325"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP8779368.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(67819..69330)
                     /locus_tag="IV281_000326"
     CDS             complement(67819..69330)
                     /locus_tag="IV281_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8779369.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(69652..71004)
                     /locus_tag="IV281_000327"
     CDS             complement(69652..71004)
                     /locus_tag="IV281_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP8779370.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(71157..72233)
                     /locus_tag="IV281_000328"
     CDS             complement(71157..72233)
                     /locus_tag="IV281_000328"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP8779371.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            72429..74174
                     /locus_tag="IV281_000329"
     CDS             72429..74174
                     /locus_tag="IV281_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP8779372.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(74454..74891)
                     /locus_tag="IV281_000330"
     CDS             complement(74454..74891)
                     /locus_tag="IV281_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP8779373.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(74875..76248)
                     /locus_tag="IV281_000331"
     CDS             complement(74875..76248)
                     /locus_tag="IV281_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779374.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(76570..77058)
                     /locus_tag="IV281_000332"
     CDS             complement(76570..77058)
                     /locus_tag="IV281_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8779375.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(77080..77682)
                     /locus_tag="IV281_000333"
     CDS             complement(77080..77682)
                     /locus_tag="IV281_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8779376.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        22337 a        16537 c        14151 g        24701 t
ORIGIN      
        1 gcgtcaataa ttttgtgtaa atggaaaagc ccattctgtt gatcaagtta aaacattgat
       61 tctaatgtat catgtatttc tttaaaacct ttatgaactc ggcatagaaa cttttgatta
      121 tagttgttga attgagatac tagaaatctc tccagagatt cttcattagg aaattgctct
      181 tttcttttgg tatatttctt taattgtttg ttaaaacctt caatcaaatt agttgaataa
      241 atagttcttc tgattgacgg agggaaatta taaaaggtta atatagcagg attcatgagc
      301 aatttaacca ctcgtggata ctgctttttc catttatcta tcataaacct tatttgatta
      361 tttgcttctt cttttgaact tgcttggtaa actaatttga aatcgctaca gatttcttgt
      421 cgatcactaa cacgaacctt atgagcgata tttctagata tatggacaca acattgttga
      481 aattgagcat ttggataaac actatgaata ctatcgtcaa taccacttaa accatctgtt
      541 acgactaaca aaacctcttc taaaccacgg tcttttagat cttgaagaat ctctttccaa
      601 acataagcag attcagttgg agctatagta aatcctagaa cctctttagt tccatccaac
      661 ctgataccaa ttactatata gacagcttct tttgatacgg tttgtctttt taagggaata
      721 tgagtagcat ccataaaaat gactgagtat ttagcttcta aagttctttc tttaaatgcc
      781 gaaacatctt cagacacaat tttactcata tttgaaatag tttgtggttt gtaataatga
      841 ccatacattt tttcgattag atcagagatt tcggacatag taattccttt ttgaactagc
      901 tgaataatag tggtttctaa agagtcattg gttcttttat aagcaggtaa agtttgttga
      961 gaaaagtctc cattcctatc tctaggtatt actaaattca attcaccgta ctctgtttta
     1021 aaagagcgcg aataattccc attacgggaa ttacctgaat taaatccagc tctatcatac
     1081 ttttcatagt caagaaatac cgttaattca gcttgtaata aagcgttaat agccaattct
     1141 aggtgacgac gaaacaaatc atccaaatca cctttattga ttagtatttc cataatttct
     1201 gtagtaaaat cgttcatatg gaagtcctct tttctgtgaa tgtgttgtgg taactctatt
     1261 ctacagaagg gacttccttt tttctattta cacaaaatat tttacactct cttcaacgat
     1321 attcttgtaa gtaattatgc cccaactagg atttgtgggt tcatttccat ctggagttaa
     1381 aaaaacaaaa atttttttat agttagaata attgcgttct atatatattt ggtatttatt
     1441 aagttgccct ccagactccg aagacagaat tttattttcg atgcacacca ctattttatt
     1501 tttttccgat accaataaaa tatcaatatt cttattttct ctgtaaacaa ttaaatcttc
     1561 atagctatta agtaaagcat caaaataatt gatagcagct ttggtatcat cattagaatc
     1621 tatatacctt ttcaaaaaca ttttaaaaaa ctcatcagat aaattgtggg tttcatttgg
     1681 agcaaacaaa aaagctaaaa aattactatg tcttatttca tttttagata gcttaagtat
     1741 ctgaaatata tttgttttcc cagtccattt agatagtttt cttcaatatc atccatatcc
     1801 tctaatagat tttttaaaca ctttttttca tctatcatgt tttttctcct tgccattatt
     1861 ctaactaaaa ttttacgtat ttgtttctat gtctacatta tctttttaac agtatctatt
     1921 tttcttttgc aacttacttg gctaccaaag caaataaaaa agttatttat aaatttctcc
     1981 ctaccactac ctattgctat tatttaaaat agggataatt ttttctctta aaaaatatca
     2041 ttatttgtgg tatggagccc ccgcattgat ccggaaggca cggtagattt gctcgacaag
     2101 gatcagacgc atcaattgat gtggatacgt catttttcca aaagagatct gagcattgct
     2161 tcttttcatg acggcttcgc tgagtcctaa agagccgccg ataacaaaaa caaggtggct
     2221 ttttccttgg atacctaatt ggtcgatttg tttagcaaaa gattcacttg taggattttt
     2281 cccttcaatt gctaaggcat agacgtattc ttgttctttg attttcgcta agatccgttc
     2341 cccttctttt tcttttactt gtctcatttg cgcttcgctt aaattctccg gggctttttc
     2401 gtcaggaact tcgatcaatt cgatttttgc ataggcattc agccgtttga catactcatt
     2461 gataccttgg atcaggtatt tttcttttaa ttttcctact gagataattt tgatattcat
     2521 gttttctctc ctttggtctt catgctcact ttgtttatta taatcaattt tttcgtctat
     2581 atgtttattt tggctatttt tgttaaaaag atttccccaa cagttatgca caacatccac
     2641 aggtttatcc acagaaaaca gtgttatttt accttatttc atcacattca ttaggcaaac
     2701 atacgtttta taagaattta tccactttat acacaggctg tggatatgtt ttatccctta
     2761 tttttccaat cctatccgcg tagttttaca cagtatctgt ggaaaaagaa aatcaaatat
     2821 tttcatctga aaacaatgat ttttcctaaa gtttttcata aaaagttcac agtctcaaag
     2881 tagactaaag tcaacaaata agactatact aacagtagat aaatgaatgt aattggagga
     2941 ggaacacatg gatagaaaaa atgtgacacc aaagatgaaa aaaagtaaaa ataatagcct
     3001 ctggcgtaag ttaggtctgg ggcttgttgg agggatcgtt ggaggactac tgacagctgg
     3061 cattttttat gcagcgatgg gcactggcaa caatagttct actgctacaa gcggcaatca
     3121 gaattctgcc ggtgaaacag ttgtggaaaa tgtcaaagtc aatgtagatt ctgatatcac
     3181 caaagcggta gataaagttc aagacgctgt cgtttctgtt atcaacttac aaagtcaaaa
     3241 ccaatcaagc ggttttggcg gactattcgg gcagcaagaa gaaagtagca gctcagatga
     3301 tagtaatttg gaagcttata gtgaaggaag cggcgtgatc tacaaaaaag acgggaatac
     3361 tgcttatgta gtcacgaata atcacgtagt agatggccag caaggtcttg aagtcttgat
     3421 gaaagacggt acgaaggtaa aagctgaatt agttggaaca gatgcttatt cagatttagc
     3481 ggtgttgaag atcaattcag acaaagtcga aacagttgcc tcttttggtg actcaagtgc
     3541 tctgaaggtt ggtgagcctg cgattgcgat tggttcccca ctaggatctg aatatgcaaa
     3601 ttctgtcact tcaggaatca tttcttcctt gaaccgacaa gtgacaagta cgaatgaatc
     3661 gaacgaaaca gtcaatatca atgcgatcca aacagatgca gcaatcaacc ctggaaattc
     3721 tggtggtcct ttagttaata tcgaaggaca agttatcggt atcaactcaa gtaagatcgc
     3781 aagtacttct gaatctactt ctaatgtaag cgtagaagga atgggatttg ctattccaag
     3841 taacgatgta gtgaatatca tcaatcaact ggaaaaagac ggaaaagtga cacgtccagc
     3901 tctaggtatc acaatggtcg acttatctgc cgtttctact cagcaacaag aacaaatctt
     3961 gaagattcct tcatctgtaa ctaacggtgt catcgtgaca aatgtccaaa cagctacacc
     4021 ggctgaaaaa gctggattga aacaatacga cgtcattact aagatcgatg acaaagaagt
     4081 ttcttctggt gtagaacttc aatctgcttt gtataagaaa aaagtgggag attctgtcaa
     4141 aatcacttac tatgaaggct cgaaagaaaa aacaactact gttgaattga cgatcgatca
     4201 atcggcatta aaacaaaatc aaaatagtga aaattaataa aaaagaggtt gtgacaaaga
     4261 cttgtcacaa cctctttatt ttggaaatgc ttctccgact acggcatcct caggcaatac
     4321 taagattcct ttttttgctg gagcatttgg caaatgcaat tcacgagcgg aacagatcat
     4381 tccatagcta tctacccctc gcaaagttcc tggccagatc atcattccat ctggcatcat
     4441 agctcctggt tttgcaacga caactttttg tccagcttcg atattgggtg caccgcaaac
     4501 gatttgcaat actgtaccat tgtctacttc tgtctcagta atcgataaat ggtcagaatc
     4561 aggatgtggg acgcatgttt ttacgtaccc gacaacgatc ttcggttcat gatcagcttc
     4621 aagctgttct tcgaatcctg cttctttgat caatcgattc aatttcgcta attcctcatc
     4681 tgttaagctt acttggccgt tcccttcgat tgttagatga tcggagatat ggaaaaagtt
     4741 ccaagcaaca acttgacctt gttcatcttt gatccgagca acattccatt tgcgttcaac
     4801 agtgttttct gttcctttat cgtcggctat aataaccatc aaggtgtctc cgacgtgtgc
     4861 tttgttataa gcaaaaatca tgttctttcc tccattatcc tttgctgaga tttatcttaa
     4921 caaaaaattg cattcactgc atgaaatttt tttcatgctt tatttccgaa gaagcctctt
     4981 ctggaacacc atttattcgt aacaagggac tgtgacaaga cttttgtcac agtcccttta
     5041 ctgttttagt taattgatta aaaaggtcgt acaagttcca cttgcttaat tacttggctg
     5101 ttcttcttcg tcagaaacga tcaagacatc acaaggtgaa tgacgtatca cgtagctggc
     5161 tacacttcct gtcataaagc gttcgactgc atttagacct gactgcccaa ccatgattag
     5221 atcgatctca tatttttttg gcaatttgac tgccatggat tcacgaacag aaccatatgc
     5281 aataattcct tctatatttg tgtaatcaac agatttccca tattctttac attcttcgat
     5341 catttccttg gctgtttcag tttgctgatc gactagattt tgatcaatag gtgcatagcc
     5401 cataaagtta taaaattgct gatcgattac gtttgctaca tatactttcc cattattacg
     5461 gcgagccact tctaccgctt ttttgaaagc agcaagtgct tgttcactac cgtcgatacc
     5521 tactaaaata ttcttatatt cttgattcat cttgacccct cctaacagat gtcagccttt
     5581 tgttacactt tctaggaacg attcgatttc ctgttttgtt ttacgatctt tattcactag
     5641 acgacctaat tcttttccgt cttggatcac gacaaaactt gggataccaa aaatgttcca
     5701 ttcagcggct acatctatgt acttgtcacg gtctacttca ataaattgcc aatcagaaaa
     5761 gtcattttca atttcaggca tttgcggttt gataaaacgg cagtctccgc accagccggc
     5821 agtaaagaaa aatacactct ttccagtttc tacgtagcct gctaattctt ctaaactatt
     5881 tgggataatc attctttgtc ctctactttc tgtttatgta tttttcaatt aaattataca
     5941 cctttctttc gaactaacaa acattgatcc tctttttata aaaaaaatct ggcaaaaagc
     6001 aatcttttgc cagattttat agtttcacta tcgcattatt ttccgtatac gatcgtattt
     6061 actgttgatt tatccaaacc tttcagtgtt tgaatcaaca tttctcgtgc agcttcaaaa
     6121 tcgcgaatac tgaacatcgt ttgatgcgta tggatataac gtcccactac gccaatcact
     6181 gtacttggga ttccttcgtt ttgtgtatga gcagcacctg catctgtacc gccttttgaa
     6241 acaaagtatt gataagggat attgtttgtt tcagcgatat ccagcaagta ctcgcgcaaa
     6301 cgaggaagca tgatcaaacc tggatcttgg atacgcatca atgtaccttc tcctaaatgt
     6361 ccgaatgtgc catttttcgt cactgtatca tctgctgcag agcagtctac tgcaaagaac
     6421 agatctggct tgaatttatt gacagaagct ttagaaccac gcaagcctac ttcttcctgg
     6481 acattcgcgc ctgcaatcaa tgtatgacct aatgtttcat tttgcaacgc ttctaaagct
     6541 tctaatacca ttgtacagcc atagcggttg tcccatgatt tactgataat attctttccg
     6601 ttggctgttt tgatcgtttc agcgtaagga acgattgtgt ctcctggaag gacaccgtaa
     6661 ctcattgcct cttctcttga ttcaaaccca gcatcgaaca aaatatcggt tacttctact
     6721 gatttttgtc cgcttgttcc tcgcaataaa tgtgggggaa cggaagaaga aatacatgga
     6781 taattcttac cagtagaggt tttaagtgta aaacgctgag cagaaacaac gtaaggattc
     6841 cagccgccta gaggtacgac tttaaaaagc ccgttttctt ggatttgtgt cagcatgaat
     6901 ccgacttcat ccatatgtgc tgcgaccatt acacggggtg catccgcttc ttcatgatgg
     6961 cgcagaccaa aaatgccgcc caaaccatct aactgtaact catctaccag cggttccata
     7021 tgactttcca tataagcgcg aacatcttgt tcaaaaccgc ttgttccttg taattctgtt
     7081 aactctttga ttcgttgaaa tgttttttct tccatatgct attcgtctaa atttcagact
     7141 aaactcctct cagacttata ttgatctgcg gataaaatat gattttttct gcagaccgat
     7201 cgtatttatt atagcttatt ttttctggta gcggttattt atttcaaaga aaatatgaga
     7261 gtgtggtaaa ataagcacaa ggagtaacaa gtgagaagaa ggaggtatgt ccacatgtat
     7321 gaagaaagcg aactaagcta ttttaaaggc gggttagcag ttggaatcgg tctaggatta
     7381 ttgagcgggg tagcttcaac gatttggtat catcgcaaaa aagtattgtc agcagaccaa
     7441 gtacttgaaa atatcaagtc atcttttcta aaagaaggtg caatcgaagg ttcttggatc
     7501 tcttttgaaa aggaacctat taggaaattc gccgttcggt cgagtgctta tcggggaggt
     7561 ctttctcgaa tagaagatgg cgaaactgtt tattatgaat ttttagcaga tgctcataca
     7621 ggaaccgtat tagaaatttc aagaaaaaaa ggtgcaacag attaatacgt caaaaaagtg
     7681 agagaacgaa tacgcaccca ctattcaaaa gagaggtatc atgtccttct tttgtagtgg
     7741 gtggtttttt agatagagct ttccaaacat atattccagt attttctttt taaataaatg
     7801 atatttagcg ggaaaaacta gcatttcctt tgattatgag gtatttttaa tttagctttt
     7861 gttttctata gttaaggaga gtgaaaaatg gaacaacaag aaaaaattgc tgttctgatt
     7921 ccttgttaca atgaggaagc aacaatttca acagtcattg cagactttaa acgagaatta
     7981 ccagaagcag atatttatgt ctatgataat aattcgaccg atcgaacata cgaattggcc
     8041 gttcaaggtg gagcaatcgt aaaaaaagaa cctcgtcaag gaaaaggaaa tgtgatccgc
     8101 caaatgttct ttgatatcga tgccgactat tatttaatgg tagatggcga tgatacttat
     8161 cctgcagaag ctgtacatgg tttgctcgag aaattacgtt ctggtgaagc agatatggtc
     8221 attggtgatc gcctatcaaa tggaacctac tttgatgaga ataagagacc tttccacgat
     8281 tttggaaata accttgttag aaatacaatt actagaatgt ataaaacaaa aattcttgat
     8341 gtcatgaccg gctatcgagg attcaatcgt atctttgtca aaagttttcc tattatgagt
     8401 tctggttttc aaatcgaaac ggaattgacg atccatgctt tagataaaaa attcaagttc
     8461 gttgaaatcc caatcgatta tcgtgatcgt cctgaaggca gcgaatcaaa attgaataca
     8521 ttctctgacg gattcaaagt gatcatgatg attattaaaa tgtttaaaga ttataagcct
     8581 ttgcttttct ttgggatttg gaccattttc ttctttttat ttggattggt cacggggatt
     8641 cctgtgattc gcgaatatat gctgacaagc tttattacac gcatcccttc tgcgatttta
     8701 agtactggat tgatgacttt agcacttctt tcattagtaa cagggttgat tttggataca
     8761 gtcgtcacga acgctaagaa agaatatgaa ttgaatctat atcatgttta tcatgaatac
     8821 cacagtagaa agtaaaaaaa ggatcaagac tgcaaaaata atttgcaatt cttgatcctc
     8881 tttttaatta attatttcgc caaacgatag atggcatctg catagattgc tgttgctctg
     8941 aacaagtcat caagactcat aaattcgttt gcttgatgca ttgtatcagt atatcctggg
     9001 aacatcgctc cataagcaac gccacgtttc aacaaacgtc cgtaagttcc gccaccgatg
     9061 atttgttcgt agcctttttc tccagtgtgg tcttcataga cttgcaataa tgtagccacc
     9121 aatggatcat ccattggtac gtaatgtggt tccattacgc gagcacctcg agtaactgtt
     9181 gcgccttctt gccccactgt ttgttcgatg tcactttgca atccgtctac tgttacacct
     9241 tttggaaaac ggaaattcat attgatgaag ttcgcttcct ctgtaccatt ttcttcaaaa
     9301 gcaaatagtc cagcattcat tgtcaatttg cccatttttt catcttcata agcgactcct
     9361 agtttttctc cataaaaatc ttcatggaca taagcagctg ctacatggat aaattgttta
     9421 gcagatccta ggaattcata gttatctaag aacaatgcca agaaagaacc tgcattgata
     9481 ccagattgtg ggcttgcacc atgtgcgcct ttaccaacta attcgatttt tacatacgta
     9541 ttgtccgctt caatcgttcc gcttactgga tttgcttcga tgaattggta aaatgcttct
     9601 tccatcgcga tagctgcatc tgcacttggt acttgaagtg ctgcagttgc tgttcctggc
     9661 accatatttt cgcgaagtcc ggaaacaaaa gatttcaaga cataatctcc tgcattgtcg
     9721 cctttgaagc gtaaagctaa agaaacattt cctttttcac cattgatgat tgggaattca
     9781 gcatctggtg agaaaccaaa atctggttct tcttcatgtt cgaaataata agccatatcg
     9841 ccccagccgc tttcttcatc gctgccgact acgaagcgaa ctttttttga tagtttcaag
     9901 tcaagttctt taatgatctt catcgcatag taagctgcca tacttggacc tttgtcatcg
     9961 cttgatccgc gtgcaaagat ctttccatcc ttgattactg gttcataagg atctgtgtcc
    10021 cagccatctc ctgcaggaac gacatccatg tgtccaaata tccctagcgt ttcgtctcct
    10081 tcgccaagat caatatgccc tgcatagtta tcgacatttt ttactttaaa tccatcacgt
    10141 tcgccataag ctaacatatg tttcaatgcg tcgcgaggtc caggtccaaa tggtgcatct
    10201 ggtgttactt ttgagtcatc gcgctcgctg ttgacacgta aaagattttt caagtcttca
    10261 agcaagtctt ctttacgtgc ttctacttct ttttgccaat caattgtcat aaagttaatc
    10321 ctccacttat ttaagtcttc gtgttcatga taccacaaat tacagaaaaa aatagttcaa
    10381 aaaatctgac aattcctttt cttatctgtt tttctctaaa aaaaatatac gctataatga
    10441 gtaaaaggag gtttttagat gctgataaac gaacaactag tgaaaagagt catcacacct
    10501 cgtccaaaaa tttcacataa aggaacattt ggacgtatcg ttttgattgg cggcaatgaa
    10561 caatatggcg atcattatga gtgctttagc tgccgtacac agcggagcgg gtctcacgac
    10621 tgtagtaact gatgaaaaaa atcggagtgc tcttcacagt catctgccag aagcaatgat
    10681 tgtagtttgg acggatactg accagtttct ttccgtaata aagcaagcag atgtactctt
    10741 gattggtccc ggattgggta cttcttctaa gagtttggaa cgtttcaaac aagtactaga
    10801 aaaccaatca aaagaacaat ggctggtgat tgatggctct gctttaagtt tacttgctgc
    10861 taatcatctt ttgctaccct atcccgaaca aactgtactt acacctcatc aaatggagtg
    10921 gcaacggttg agttctctac cgatacaaga acaacgggat aaagaaaaca aaaagaagca
    10981 acaggaaatc ggtgctgtgc tcgttttgaa aagtcatgag acaaaaattt atggtcacca
    11041 gcacttctac accaatcctt taggttctgc tgctatggca acgggcggca tgggtgacac
    11101 tttagctggg atgatcacag gatttttagc ccagtttcct caaaatgacc aaacgattgc
    11161 agcagctatt tatcttcata gtttcattgg agaccagctg gcaaaagaac aatatattgt
    11221 tttgccgaca caaatcagcg aagcacttcc gaaatggatg aattattttt gtaaaaaatc
    11281 aatggaaaac gaggcatgag tcatggagaa atttacaggt tatgtgcgag aaccttttta
    11341 ttatgaaaca gatcaaatgg ggattattca tcacagcaat tatatccgct ggtttgaaga
    11401 ggctcgggta gccttattgg tacatctagg gtttccctat aaaaaaatcg aagaatctgg
    11461 gatcatcatc cccgttctgg aagtaaattg cagctataaa gaaatgatcc attacggaga
    11521 aaaagtgagc atccatccaa ctatccaaaa gtatacgggg acacggttgg attttagcta
    11581 cgaaattgtg ggaactgaag acaaaaaatt aaaaactact ggcagtagta aacactgttt
    11641 tttactttct gattcgcaac gattagtgaa gctgagcaaa gtcaatccgg agttagatca
    11701 gttattccaa gcgtacgctt ctttggatga tgtaaattct taataaagac cgtctaagca
    11761 tttttcttgt attgtgtcga gtagttcgta taatgaaaag aaaaaagcag gtgatcccca
    11821 tgtatgtcgt taaagtaatg cacggttaca tcgataaaac tggctgtcgc actcgggaaa
    11881 aaaacttaga caatctgctc attttcaaag acaaaaaaga atccgaagct tttgctaaaa
    11941 gaatcggcgg tcgtgtcaaa ccgatccaag aggtacgacc agattgattc aatatttttc
    12001 ttttcgaggc tctaataggc atgcctatca gggctttttt tgcagactgt gtttttatgc
    12061 ccacacgatt tgtaagcgat ttatgttata atagattata tctgcataga aaggaaggtt
    12121 aaatggttat caataatttg caacagcaag cttatgaatc tatacgtaaa caaatcattt
    12181 attccgagtt agcacctgga agtaaaatct ccgataaagt gttagaagaa caactagcta
    12241 tcgggcgtac accgattcga gaagcactga tccaactgcg caatcaagag cttgtaaaaa
    12301 ccattccaca gtccggcact tatgtttcaa aaatcgatat ccgatctgct tcattagccc
    12361 gttatacgcg ggaaaagttg gaaaaaccga tcctgcaaga atgcagtgcg aaaatgacca
    12421 agcaagatca agctaaacta gaaaaaatac tagaacaaac cgatcaggca gtaatcgctc
    12481 aaaataaaaa agcttttttt gatttggata ctgattttca ccgaacttgt tatgaaattg
    12541 ctggaaaacg agaaatttgg gattggctag aaagttatag tacgcacttg aaccgtttca
    12601 gatggttacg tttgacgatt tcagaattag actggggtcg agtattagat gaacatcaga
    12661 caatgcttca atcaatgatt gatcataact ttgatgaagt cggatttctt tgcacgatgc
    12721 atttacatat gattatcgag gagcaagaat acgtgattca taattacccc gattattttg
    12781 aagatattca ggacagaccc atcaaataag aggttttgga atcagcgttt tgacctgagt
    12841 attagagaaa gaaacggcaa aatagttcct catattttgc cgtttttttt gagctaatac
    12901 cgagggtcag ctgattgcac accgtttatt gagaggttgt gaaaaagctg ccctgcatca
    12961 agtaataaga acagccaagt aaaaatagct tcttatgttt ttaacttggc tggacttatt
    13021 accgcagatg caagcttttg aacaccgttt tccaagaggt cgtgagcctg ccgttcatct
    13081 ccgagtcaca aggaacaatt tcaaaaaaca gcttctcatg tttgttgaat attttgactt
    13141 gtgaccgagg agttgacagg agaacatcgt ttattcggaa aaaaaggact gtgacacgac
    13201 ttttgtcaca gtctctttat ttgtaatcta aatcatactc cgtacatctt ttttctattt
    13261 tttttatttc ttctaagatc ccttggctac gaatttcatt atatggccaa agtaagactg
    13321 cctctaatac ataaggcgaa aaatcgcttt gattcatctc ttttgaaaaa ctcatcgctg
    13381 ctatctcgct caatgcacga atgtttgatt gatgtgtata aaaaggcaac cgccacagca
    13441 caattttttc gctttgcgta taaattccct taacagaagc ttcgatgtgg tgaaatagtt
    13501 catgtccaat aatcaaagct tgcgctgttt ctttagagat cgaagctagt cctgaacatt
    13561 ttaatttttc taacggctcc gtcatcaatc gaatcccttc atcttctgca taagttgcaa
    13621 aagtgattcg ttcgtttatt tgctctgatt cacaggaaac gattggaaca gccatttgtt
    13681 cacaaatttc tcctacatcg gcaaaagggt actttttttt gatgctacat gccgtttcga
    13741 taccacaatc gattgattgt tgtataagaa ttttctgctt ggacaaagga atctttttcc
    13801 aaaataaatc tcgccgaaac tggtagaatc cccaaatttc ttcggggatc tgttgtaatt
    13861 cgtttagtaa ctcttctaac ttatgtttgt ttgatatttc cggcaacgac gatcacttct
    13921 tcctccggtt ccatagtaga tacttccaga tccatcaaaa gctgtagttg ttccagatcc
    13981 gtcgctgtaa aatcagagac tcttgctcct aagcagagat aaaattctgg tcgggcaatc
    14041 aattcagttt gataaactgc catatcttcc caaagttgtt cgacagctgt catataattg
    14101 gctgcagttg ttttcataca tgaagcatgt ccacgttgga ccttgataaa tcctttttga
    14161 tcgatgatcc gaagacttcc agcatttttt gaacgattct gttcgccgca tacataaaag
    14221 aaaggtgtat tttccaataa gcaaacttca tttttattta atcgcatatc ctctgcagca
    14281 agctctagtg cttcttctgt tgtgatctcc ttggttagat cggttgcttt cacttccgta
    14341 gaaccagtag cgatagccgt tacttttgaa gtctgtggat cgatttctac gtggacttcg
    14401 atcgattctg gtgtagcgcc tgattcaatc gctttattca tcgcttcatt tttcaacgat
    14461 cgtatatctt ctttacttgg cgaagggatg atacgttcta caacatctcg gaccattgca
    14521 agtgctacac caatagaaga aatcacttcg gcgttttcag gaatcgaata tttcacaccc
    14581 attttattac taaagtaagt aatcaatgag gcagcgccgc cccctacacc tacaagagaa
    14641 atctgatctt tctccaagtg atacttgtct gccaattcta aaatcaccgg ttcaatctta
    14701 gcataggatt tttccatgat ctgttctgcg atgtcttcaa ccgtcgtatg acaataatca
    14761 gctaatgctt ggatggcttt tcgtgcagat gggacatttc cataagaaaa atgttcttct
    14821 tgcactaatc caaggacatt ggctgcacat gtatttgtaa tagtcacttc ttctccgtct
    14881 tccatcacta ccttgacgta atcagcaggg tctccttctt taggagagaa aaattcaacc
    14941 ttaggacctt tgattttttc tgtctccgta aatactgcat aatctagacc ggcaatatgt
    15001 gcagaacgtg gaccgacatc gatgattcct gactgattag cccgtaccat cgaaccgcct
    15061 gccacaccta gaacccgaac atctaatgaa gagatatatg ttgggtggcc acctacaatt
    15121 gaataatcga ttgctggacg tccattttta atgaccccga tattggttgt tgttccgcct
    15181 acttcaaaat aaacaccatt agacgctctc aagtacatca aagagcccat gactgatgct
    15241 gctggaccgg aaagcatggt taaaacaggt cgttttttca tttcattgat ttccattact
    15301 ccgccgtctc cgcgcataat catcaaagaa acattgaccc ctgcttctcg tacggaatct
    15361 tctgtagagg ttgccgtatc tagcattttt ggtaaaatag atgcattgat tgctgctgtc
    15421 cgagttcttc ttgtcaatcc atataatttt gtgatgtcag aagccatcgt tgtagggatc
    15481 gattttaccg atgctgcttc ataaacaact cgttctggtt ccccattatc tactccaaat
    15541 gccatagaag aaaccaagac ctccgctctt tctctttcta aagaagaaat cgtttctgaa
    15601 acacggtcta catttaagtg tttgacaggt aagaatgcat tgacaatctc tatttttttc
    15661 ttattcccta aatcgatatc gttcaatcta gtttgtcgtt ttgctaaaaa accttctaat
    15721 ccaccttttg ccatcccgat tacgccgact tttgctacgt ctccttcaat cagtgcatta
    15781 gtcgcttgtg tggtagaatg tgcaacaaac acaacatctt ctggactgat attattttcc
    15841 agcaaacaat tttgaaaaga ctgaacgaca cctgcggcta ctccacggac atcatcgtgg
    15901 gttgttttca ctgaagattt tccgatgatt tcgtgcgtcg cattatcgat tgcgactgct
    15961 ttcgtatgtg taccgccaac atcaattccc attcgcacaa aacgtttcat tttcttttcc
    16021 tccattcatc taatcgtttt tatcgacgtt ttgtttacta ggcgttcccg tacaataaga
    16081 acgtgatcag gcataagatc gctccgatga tccaaccagt tgggatggat ttcttcatat
    16141 attctcgaga agttactttt gtataaccta atccccaagc aacccaagat tgtgtaatat
    16201 ctaaatgctg aggagcaatc gttgtgattg caaataacgg gaacaaaaag gctggcgaca
    16261 tcgttggatt aacagctaat acgacagcta aaatagctga gcctgaaccg actagattca
    16321 ttggaccgcg gaaaaatcct agtggtgtca atatagcaaa tacgattgcc aagactaatg
    16381 gtgattgcgg tatccaatct ccaaaaatag ctgaaaaata aggagaagca tacgcagctg
    16441 aattattgaa catcgctaag gtcatcaaga aaccaaccat tggcgcaaca tctactgcgc
    16501 catctgtaaa taatttcgca aactgacgac agatttcttt ataacctccg cgcaattttc
    16561 cagtaaacag caatgcccag atacctgcta gaataaaacc taagatgatt ggaagatcta
    16621 aaagaacgac ccctaaaact ggtaataata cggctaacca tgagatcatt ggtgcgtcgc
    16681 caccttcagc tggtacgttt gctgccattg catagcgttt ctttttgttc attgatatat
    16741 tagcgaccgt tagtaccaca actaagctta caatcatccc aatcatacca atttggaaat
    16801 agtcattata agtatagctt tcagccgctg gattgaaacc agcataaatc gtttgatatt
    16861 gtttgaaatt gacgatatta gcaaagattc cagccataat cgagcccata aatgagaaca
    16921 gtgcaaccgg tactgaaata cctaatgaca tcaagattgg caacacaata actgcaatag
    16981 aaatcactgg tccgattcct gtcatcgaca tgaatataac agcggttaca atacatagta
    17041 agctcatcgt gattcttggc ttgtcccctc caagttcaac tacctttcga atcagggtag
    17101 ctgcgattcc gctatctact agaacacgcc cgaaaaaggc accaaagaag acatttacca
    17161 agatcgattt ggcgtactca gctggccccg tcgtatagat atgcgtcaac acatcaatta
    17221 ctccttgccc ttccatcgcc gggtttggtg aaaacgtgtt tccgactagc gctaatccca
    17281 tccatacagt agccatgatg gcaaagccga ccatcaagtt gtagcctttt acacagtacc
    17341 agaccatgaa aaagaatgag ataactaaca aaataccaat aataatatca ctcatgttat
    17401 tcctccgtcc attttgtttt tctgataagc aaactaatat atcagtcaaa ctcattctaa
    17461 cttatgtagt tagcgctttc attaaagggt gaccataaat gttcagtttt caattaaaaa
    17521 accttaatta tgctatttta gacttatacg ttattgtcct gtttcatttg atggaggatt
    17581 gtaaataaaa aaagaggaaa gaacataact tttcgttatc ttctttcctc tttataattg
    17641 tttagcttat ttagccaaag cttcttttgc ttggtcagcc aatgcagtaa atgctgctgc
    17701 atcgttaaca gccaagtcag ctaacatttt acggttgatg tcgatttcag caactttcaa
    17761 accgtgcatt aattttgagt agctcaagcc attcatacga gctgctgcgt tgatacgagc
    17821 aatccacaat ttacggaagt cgcgtttctt ttgacgacga tcgcgatatg cgtagttgta
    17881 tgaattcatt acttgttctt ttgctgtttt aaataaagtg tgttttgaac cgtagtaacc
    17941 ttttgctaat tttagcactc ttttacgacg tttacgagtt actgttccac ctttaacacg
    18001 tgccatgtat aattcctcct aataaaaata aatcttcaga ttctgttatt tgtacttctt
    18061 atttcatttt tgctagttgt tggcgaatac gtttgaagtc gccactagaa accatgcttg
    18121 ctttacgcaa ttgacggcgt tgtttttttg ttttgccgtg gaaacggtga cttgtaaacg
    18181 cgcggaagcg tttcaatccg cctttaccag tacgtttgaa acgttttgct gatccgcggt
    18241 gtgttttttg ttttggcatg actatatttc ctcctcaaaa tctttcgtta tccgactatc
    18301 ggctgtccaa cagccagtta gtcattcaaa ttacttactg tcgtttttcg gtgccagcgt
    18361 caagaacatg ctgcgtccgt ccattttcgc tttttgttcc actgttgcaa tatccgcagt
    18421 ttcttcagct aagcgattca agactttttg accaatttct ttatgggtaa tggcacggcc
    18481 tttgaaacgg atagaagctt tcactttatc gcccttttcc aagaacttac gtgcattacg
    18541 aagttttgta ttgaagtcat tcaagtcgat cgtaggactt aagcgaactt cttttacatt
    18601 gatcactttt tgttttttac gcgcttcgcg gtctttcttt tgttgttcga aacggtattt
    18661 accgtagtcc atgattcgcg caactggtgg tttcgcacca ggggcaacta acacaagatc
    18721 caagtttgct tgttctgcaa tattcaatgc ttctgctttt gtcttaactc ctaattgctc
    18781 accatctgaa ccgatcaatc gtaactctcg tgcacgaatg ccgtcgttaa ccatcatatc
    18841 ctttgctatg gtcattcacc tccaaatatt atgagagaaa gaaaagctta ccgaataaac
    18901 ggcaataaaa aaacgagttc tcttacagaa cccgcgcgta cttcacctaa tagaaccagt
    18961 gaaaactatc tagcccagtg acattatcaa ctaaggcgag aagcgggtgc ttctgctttt
    19021 atttctcaac catcatagta tagcaaaaac taatcttttt gtcaatgttt ttcatacagt
    19081 ttgtcttcct tttctgaaaa tgattagatt agcagacttt ctagcagtga ttcccgcttc
    19141 tttgctatac taaagaaaaa cgaggtgaac ccacatgaaa aaaatcgaac ctattccacc
    19201 ttctcttcct cttcttacaa agggagagtt ttatttagaa gatgaggaga tcttctctgg
    19261 tcttctgatc aacgggggtg accaaagcta tctcgatgcc aaaaatatgg tattaagaga
    19321 gagtcatatc caaaaactta cgatgcaaaa aacagtttta cgtcattttg aatgcagtaa
    19381 tgtcattttt gaaaaatgtg atttttccaa tctggagtgg attggtggca gtttccacca
    19441 agtcgtgttt catcaatgta aattaacagg tacgaatttt gccgaaagtt atcttcgaga
    19501 ctgtactttc actgattgta ttgcttcgat ggcttccttt agtagtacaa acttgaaagc
    19561 tgtctccttc gatcattgtc aattagaaga cagcgaattt tatgaggtca cttggaaaaa
    19621 cctttttttg tctgacaatc agttgaccgg ttcgaactgg tttcgaactt ctctgaaggg
    19681 attagacttt acgactaatt tcttcacagc aatcgcttta tctcaagatt ctttggcggg
    19741 actaatcgtc aaccaagagc aagcacttgt gatcgcccaa gcccttggtt tgcagatcaa
    19801 agactagatc aacgaatccg ccgccacagt gcaataagca tccaaataga taagaccaaa
    19861 ctgatgatca aggcgattcc ccatccgaaa acggaatggg taaagggtaa ctggagattc
    19921 attccgaaga atcctgtcac aatagttgga acggtcaaga tcagcgacca gaccgtcaaa
    19981 aatttcatcg tgtcattcaa gttgttattc agcaaattat tataagtgcc agagagctgg
    20041 tctaaaatct gcgaagctaa gttcgtcatt tcaactgctt gtcgtgcttc gatcagcgca
    20101 tcgtccaact cttctttttc cttttctgac aacttgcgat aaatagccaa tgccttgatt
    20161 tgttctaaaa gtactacatt ttgttttgtt cctgtaacta agtaaaccaa accgatctct
    20221 aaatctgaaa gttgcagcag attcttgttc gttgtttttt ctcttaattt cagattaagc
    20281 cgctgacgtt cactattcac ttcctcgatt aaaggaaaaa aggaatcaga aatcaaaaac
    20341 aatgtacgaa acaataaact gtatacactc atgtctggca ttcgttccaa aagcgatcga
    20401 attagtcgaa tcacgtactc ggtattcggc gtggtaaatg taaacaaacc gtcttctttt
    20461 aaaataaaag cgataggact agtttcataa tgattatcga ctttttgctg gtgcggaaca
    20521 tttgatacaa gcagtaacgc ttcatcaaaa gaatcgtact ctacgcgcgc ccgttcattt
    20581 ttatctagag aataagtcag catctcgtca ctaatgccat attcttcttt taagttttta
    20641 acagctagca catcgttttg ctgtacattg atccaaaaat tacggtcttg attaaaatag
    20701 tgtttttccc gcatactcta tcctcctgac atttgtttcc agtataaaga aaaaagtcgc
    20761 tcaaataaag gtttaccctt tatttgagca actgtaaatc aaatggctac tttggtttgg
    20821 cgaatctctg ctaatttttc ttcgatcatt ttcaagactt cttcttttgc ttgaggatct
    20881 tccacaaaat tatagcggtc tccatcgatt tggatttttg gactttcttt gtattcttca
    20941 taccattgat cgtaacgtct gttcaattct ttgtaataat cgtatagact tggatcaaag
    21001 gacaattgtt catacgaacg accacgtttt tcgatacgct ccaacatcgt atcaaaagat
    21061 acacgaatat gaactaataa atccggatgt tttttatggg cagcataggg aagttcttcc
    21121 atcatgtttt ccagcagttc atcatacaca cgaacttctg tttctgttgc tcttcctaga
    21181 tctgcattta gatggaaaag cagtgaatct tcataaatcg aacggtccaa tacattattg
    21241 tcatctttca ttgcttgttt gatgcttgca aaacgtttat tcaaaaaata aatttgcagc
    21301 aaaaaagcat actgttcagg gtttgcataa aataagggca agacttcatt atcatcaatc
    21361 gattcataaa atgcctgact atcaaagtgt tcagccatca tttctgttaa acttgatttt
    21421 ccggcaccaa ttgtacctgc taaaacgatc acgctcattt acctccagct cacttcatca
    21481 ctaaatattg tgtttaactc caaccttttt aaatataccc ccaatatcta gtagtgtcaa
    21541 ttgtattttt ttctagtcaa agaagaaaaa aagtcaaaaa attcttaaga aaaaagtgtt
    21601 ttttctttag aaaacgtatt cttagtttta cttctgagat ggttttagta aaataaagga
    21661 ggaatgtttt ttgagaccct tagtggacaa aaaaattcgt gaagcagcgc aactcgtttt
    21721 gggcgcttta ttttttgctt aaaaatcact tttacgtgag aaggagttag attatggcaa
    21781 actatggcca aggagaatca agcggaaaga tcatattgat gggcgagcac gcggttgttt
    21841 atggagaacc agcgattgcc tttcctttct atgcaacaaa agtcaccgca ttccttgaag
    21901 agctggatgc aatggacgat caactggttt cttcctacta ttcaggaaat ttagccgaag
    21961 ctcctcatgc attaaaaaat atcaaaaaat tattcattca cttaaaaaaa cagcatgaca
    22021 tccaaaaaaa cttgcaactg accattgaaa gcacgattcc tgctgaacgt ggaatgggat
    22081 caagcgctgc agtcgccaca gcagtcactc gtgcttttta tgattactta gcatttcctt
    22141 tgtctcgtga aatactatta gaaaatgtcc agctttcgga aaaaatcgcc catggtaatc
    22201 ctagtggaat cgatgcagcc gctactagca gcttgcagcc gatttatttt acaaaagggc
    22261 atcctttcga ctacttttct ttgaacatcg atgctttttt gattgtcgct gatacaggaa
    22321 tcaaaggaca aacaagagaa gccgtcaaag atgtcgctca cctctttgaa acccagcctc
    22381 atgaaactgg acaaatgatc caaaaattag gatacttgac gaagcaagca aaacaagcaa
    22441 tcattgaaaa ttcaccagaa acgttggcac agacaatgga tgaatcacaa tcacttctgg
    22501 aaaagctgac aatcagcaat gattttctta atctattgat ccaaacagca aaagataccg
    22561 gggcattggg tgccaaatta actggcggtg gacgcggtgg ctgtatgatt gcattagcac
    22621 aaacaaaaac aaaagcccaa gaaatcagtc aagcacttga agatgcgggt gctgctgaaa
    22681 cttggataca aggattagga gtacatacct atgtttaaag gcaaagcacg cgcatatacc
    22741 aacattgctc taatcaaata ttggggtaag aaaaatgaag aactcatcct tccaatgaac
    22801 aacagccttt cgttaacatt ggatgcattt tatacagaga cagaagtcat cttttctgat
    22861 agctatatgg tagatgaatt ttatctagat ggcaccttgc aagacgaaaa agcaacaaaa
    22921 aaagtcagtc agtttcttga cctttttcgt aaagaagccg gcctctcact aaaagcttca
    22981 gtaatcagtc agaatttcgt tcctaccgca gctggattag cctcctctgc cagcgggcta
    23041 gctgctttag caggcgcttg caatactgct cttaagcttg gattagatga tctctctctt
    23101 tcaagatttg ctcgacgcgg gtctggttca gcttgccgaa gtattttcgg tggtttcgtc
    23161 gaatgggaaa aaggccatga cgacttaagt tcttacgcta agccagtccc ttccgattct
    23221 ttcgaagacg atttagcaat ggttttcgtt ttgatcaacg accagaaaaa agaagtgtcc
    23281 agcagaaatg ggatgcgtcg gacagtcgaa acatccagtt tttatcaagg ctggttagat
    23341 tccgttgaag gggatctata tcaattgaaa caagcaatca aaacaaaaga tttccaactt
    23401 ctcggagaaa cgatggaaag aaacggacta aaaatgcatg gaacgacttt agctgcccag
    23461 cctccattca cttactggtc tcctgacagc ttaaaagcga tggatgcggt gcgacaattg
    23521 agaaagcaag gcattccatg ctactttacg atggatgctg gccctaattt caaagtttta
    23581 gtggaaaaca gccatttatc tgaggtacaa gaaacattta ccaaattatt tagcaaagaa
    23641 caagtaatta ctgcacatgc tggtccagga attgcgatta ttgaataaag ggagattttt
    23701 atgattgaag tatctgcacc aggcaaactc tatattgccg gagaatatgc agttgttgaa
    23761 acaggccatc cagcagttat cgctgcagtc gatcaattcg tgacagtcac tgtagaatct
    23821 gcacgaaaag tcggaagtat ccaatctgca caatatagtg ggatgcctgt acgttggaca
    23881 agacgcaacg gagaattagt tttagatatt cgggaaaatc cttttcatta tatccttgct
    23941 gctattcgct tgactgaaaa gtatgcacaa gaaaaaaaca ttcttttatc cttttatgat
    24001 ctgaaagtaa caagtgagtt agacagttca aatggccgga aatatggatt gggttctagc
    24061 ggtgccgtga ccgttgcaac ggtcaaagcc ttgaatgttt tttacgcgtt gaatttatct
    24121 cagttggaga ttttcaagat tgcagcacta gccaatttag cagttcaaga taatggttcc
    24181 tgcggcgaca tcgctgctag ctgttatggt ggctggatcg ctttctcaac cttcgaccat
    24241 ccttggctcc aagaacaaga aactcagcat tctatcagtg agttacttgc cctggattgg
    24301 ccaggtctat ccattgagcc attgattgct cctgaagatt tacgtttatt gattggttgg
    24361 acgggtagcc ctgcctctac ttctgatttg gtcgatcaag ttcaccgttc gagagaagat
    24421 aaaatggtgg cttatcagct tttcttaaaa aatagtacag aatgtgtcaa cgaaatgatc
    24481 aaagggttta aagaaaataa tgtaacgttg atccaacaga tgattcgaaa aaaccgacaa
    24541 ttactgcatg atttatctgc aatcactggg gtcgtcatcg aaacgcctgc tttgaacaaa
    24601 ttgtgtaatt tagctgaaca gtatgaagga gccgcaaaat cttctggtgc aggtgggggc
    24661 gattgcggaa tcgtaattgt tgaccagaaa tctggcattc ttcctttaat gagtgcatgg
    24721 gaaaaagcag aaatcactcc actgccgtta catgtctata gcgatcaaag aaaggaaaac
    24781 cgatgaatcg aaaagacgaa catgtttcac tagctaaagc ctttcatgat aaacaaaaaa
    24841 acgaatttga cttcgtacgg atcatccata accctttgcc gcaaatagca gtttctgatg
    24901 tcgatctaag tacacaggca gttgggttta cactaagcag cccattttat atcaatgcga
    24961 tgactggcgg aagcgaaaaa acaaaaaaaa tcaaccaaga tcttgcaatc gtcgcaagag
    25021 aagcagattt gatgattgcc actggttcag tcagcgctgc cttgaaagtc ccttctttag
    25081 ccgatactta tacgattatg cgacaagaat atcctcacgg gaaaatcatt gccaacatcg
    25141 gtgcagggac ttctgtcgaa agagccaaag aagctatccg attgtttcac gcagatgcct
    25201 tacagattca tttgaatgca cctcaagagt tggttatgcc tgagggagac cgtgatttta
    25261 ccaattggaa agtacttatt caagaaactc aaacagccat tgacgtcccc ctcatcgtaa
    25321 aagaagtcgg ttttggcatg acaagagaaa cgctcaatga tctagctgct ttaggagttc
    25381 atacagttga tatcagcggt cgaagcggaa caagcttcac ccagatcgaa aacgcccgtc
    25441 gctccaagcg ggaactgagt tatttagctg actgggggca atcgacagtc tcttcattgc
    25501 ttgaagcaaa tgaagcagac acttctatgg aaatcctagc ctccggaggt atccgcaatg
    25561 cctatgacat atttaaagcc ctttgtctcg gcgcaaatgc cgtgggtact tcaggaaccg
    25621 tactgacgca tctgatgaat cacggcgtag aagaaactat tatactgatg aagcaatggc
    25681 aagaagagct tcgtctgctt tatactatgg tcggcgcaac gaataccgcg gccctacacc
    25741 aacaatctct gatcttttcc ggaccagtga aggattggtg cgaagcaaga gggatcgact
    25801 tagtgaagta tggaagacgc acagaaaaga gcgtgggaca aaagtaaaga acacttttgt
    25861 ctcacgctct aaacacgtac aaacggcgga agtaaaagca actccttcgg aaataagctg
    25921 aaattcacaa aaatttgaaa agcaattttc gtgaattcct tcttatttct tggagttaaa
    25981 tgcttctgtc ccagcctctt ttttcgaata aatgatgttt aatcagctgt ccttcggtat
    26041 tcgttcaatc cgctgatttc acaacctctc cactaaacag tgtactaaag ctcttttaag
    26101 gttgttttcc gatataggca agtatgcctc cgtccacata caagacatgg ccgttaacga
    26161 aatcagatgc gctagaagcc aagaagacag atggtccagc caaatcgatt ggctcgcccc
    26221 agcgtgctgc tggcgtacgt ccaacgataa attgatcgaa tggatgacgt tcaccgtttt
    26281 cttgtgtttc gcgtaatgga gcagtttgcg gtgtagcaat ataacctggt ccaattccat
    26341 tacattggat attgtattga ccgtattcag aagcgatgtt tttcgtcagc atttttaatc
    26401 cgccttttgc cgcagcataa gcactgaccg tttcgcgtcc taattcactc atcatggagc
    26461 agatattgat gattttaccg ccgccttttt cgatcatatc cggaataact gcttttgaaa
    26521 cgatgaatgg ggcatttaaa tccacgtcga ttacttggcg gaagtctgcc gcatccattt
    26581 cgatcatcgg tgtccgtttg atgatccctg cattgttgac tagaatatca actgaaccta
    26641 catcttcttt gatctgttga accatcgctt gaaccgcttc ttcatcggtc acatcgcaga
    26701 cataaccttt tgcatcgatg cctgcttctt tataatgcgc tataccttca tcaactgatt
    26761 cttgattcaa gttattaaat acgatttttg ctcctgctgt agctaaagag cgtgcaatct
    26821 caaatccaat accatagaca gcaccggtga ctaatgctac ttttccatct aaacgaaaca
    26881 tatccatact aaattccatc attttctctc ccttatgctt attttaattg atccatttgc
    26941 accatatcca tgtctgtata ggtgatattt tctccgcaca ttgcccagat gaatgagtaa
    27001 ttgcttgtac ctacacctga atggatcgac cagcttggcg aaataatggc ttgttcattg
    27061 tctacaacta aatgttttgt ttcatctggt ttgcccatca tgtggaacac acgtgtatct
    27121 tcattggcat aatcaaagta gacataagct tccatacgac gttcatgtgt atgacaaggc
    27181 atcgtattcc aagcgcttcc tggttctaaa atcgtataac ccatttgtaa ttgacagctt
    27241 tcgcatacat ttggatggat gtattggtag attttacgtt cattcagtgt caggccttca
    27301 ccagtctcca ttggttttac ttggtcgata ctgattttta catttggata tttatgatga
    27361 gctggaacag aactaatata gaatttagct gggttttcag catcttgaga agaaaataca
    27421 acatgtcgtg tttcttttcc gatatagtat ccgtcttgtt ttttcatttc ttctttttgc
    27481 ccatcgatct cgatgaatcc tggtccaccg atattgatca cacctagttc acgacgctct
    27541 aagaagtaat caacacctag ttctttgtct aatgtgattt ctaattcttt ttcagtaggt
    27601 gtcactccac caaagatcat tcgatcgttg tgcgtataag tcaaactgat tttccctggt
    27661 tcaaatacct tttctactaa aaattctttt ctcaattgtt ccgttgaata atgacggata
    27721 tcttctggac tgtgtgtata acgtgtttcc atttcttgca ttttcttcta ctccttctat
    27781 cgaatgattt ttccagagcc gcatgctaaa aattgtgcga cttcttcttg actaaattgg
    27841 ttgcagtccc catggatcgt atgcttcaag gcagaagctg ccgtagcaaa atcaatgatt
    27901 tcttgcggag acatattgga taaaatccca tggagcacgc cacctgcaaa agcatctccg
    27961 ccacctactc gatccacgat tggggtgatt ctatggactt gggattccac atattcatgg
    28021 ttcatccaca atgtcccctt caattcattt tcacttgctg aatgaacggt acgcttggtc
    28081 gaataaaaca cctgaatatt cgggaacttt tcctgcattt tttgataata ataaattaga
    28141 ggttgttctt ctgcttctgg cgctttctcg atacctaaca aataaagagc atccattttt
    28201 ccagctgagc agatatctac taaaggtaaa agctgatgga ttacttcgct ggcttcttgc
    28261 tgtgtccata attttccacg atagttgata tcgaagctga tcaggcagcc agcttttttt
    28321 gcagcttcta tcacctgttt cgtcattttt tgccattttt tagacaaagc tggggtgatt
    28381 cctgaaacat gaaatatatc tattccttgg aataactcct ctagcgacca ttccaaatga
    28441 tccatttcag caaaacttga tccggcacga tcatagatca ctgaggcggc gcgttcgcca
    28501 ctaccagttt ccatataata agtacctagt cttgatccac cagataatac ctgtttcgta
    28561 tctactccat aacattgcaa gtgctttttg actgcttttc ccaatgcgtt atcaggtact
    28621 tttgtagcaa aaatcgcttc atgtccgtaa ttagctaaag aaattgctac gtttgcttcc
    28681 cctccgccat aatgggcagc gaatcgttca cttgaatcga tacgggttcc ctgctctgtg
    28741 gacaatcgca acatgatttc tccgagtgtc actacttttc ccataagcag ctacctcttg
    28801 atttctttca tttttgctgc atactgttgc gcgacttccg ttactttgtc aaaatctcct
    28861 gtagctgcgg gtgctaaaag gttcccacct actccgactg ttacgacacc agcatcgaac
    28921 cactctttca tattgtccag actcacaccg ccagttggca tgatattgac ttgaggcatc
    28981 ggcgccttga aggcagaaat gatacttggt ccataagcgc ttccaggaaa tagtttgacg
    29041 atatctaccc cgcttttcag cgctgttttg atctcggtaa tagtcataca tcctggtaag
    29101 taaggaattt gatacagatt gcacatctct gccgtttctt gatcaaagga agggctaaca
    29161 atatactctg cgccagctaa gatagccaag cgagctgtga cagcgtctaa tactgtgcct
    29221 gcgccaatca ctatttctgg ttggtctttg tagaatgaca aaagttcttt gattacttga
    29281 tctgcttgag ggactgtaaa agtcaactca ataccagtta gaccaccttt gatgattgcg
    29341 tggcaagcat tcaaagcttc ttctttgctt tttccgcgta ccaccgcaat cactccggcg
    29401 tttttcaatc gagataaaat atcaattttt ttcatcttct ccacctattt cctatttaga
    29461 aattttattt tataacttgg aaatctatac ccatattata cggcagaaaa gaaaaattgc
    29521 aaacgctttt ttcattattt ttgaattctt tgctcgattt cgctttttgt ttgcttgagg
    29581 gcactgatta tttgttcttt tgtctcatct gttaaacgat atttcggcat acttacacta
    29641 aatgcaccaa tgatttgttc gtttttcacg attgaggctc ctatacaaaa aatatcacgt
    29701 tcgatttctt catcgtcaaa tgcgatgtgg gtttctttta ccgttttgat ttcctttttc
    29761 aaccgtaatg cattggtgat cgtatattct gtgtaaggga tcaacggatg cttttcaaga
    29821 taatcagtgt actgctcttc actgaattct gctaatacgg ctttgcccat cgcagaattg
    29881 tataatggtc gggtgattcc gatttttgaa gacatgcgga tcgtttgatg ttttggatct
    29941 agtttgttca cgtacaagat ctcattgttg tccaataccc ctaagtggat agtctcatcg
    30001 atcttttgct gcagctgttc taaaaacggt aaagtcaact ctaccaaatc gatttgttca
    30061 atgtttttat tcgcgtagcg aataagcttt gcacctaaac gatagttttt ctctgaattt
    30121 ttatttacgt agccgatcat caacaaagta tctagtattt tcaagactgt agaagaggtc
    30181 attcccacac cctttgcgat cgtctgcaaa gagacatctt ggttttccgc taaatagtct
    30241 agtattttag aggctttgat caatacagtt ccataaggct ttttattttc catataatat
    30301 tctcctattt ctttataaga aaaattatag cggaaatacc atcaatttaa aagagatatc
    30361 cagatataaa ctaccatttc tctttacagg caatttaatg ttgaagatcc gcttcgtttt
    30421 tacatcaaac tttgatataa agcaatgatt tcttctactg tcgtttctct tggatttcct
    30481 ggtgtgcata catctcgcaa agcatcttct gcaattgctg gaatatctgc ttcttttaca
    30541 cctaattcag aaatggtttc tgggattcca accgctttgc tcaaccgatc gatctcacca
    30601 caagccgctt ctcttacatc ttctaaagac atttcagctg ctcctttaat ccctagaaca
    30661 agagcgatct cacggtactt ttctccagta tattctttgt tatatttcat gacagttggc
    30721 aaaagtgctg cacacgctac gccatgagga atgttgtacc atgcacttaa cgggtgagcc
    30781 atcccgtgta ccagtcctaa accgacattt gagaagccca ttcccgcaat atactgtcct
    30841 aatgccattt tttctcgccc attttgatcg ccatctacag aagcttccaa agaatggccg
    30901 atgatttcga ttgcttttaa atgcagcata tcagtcattt cccaagcgcc ttttgtgata
    30961 aacccttcaa tagcatgcgt catagcatcc atacctgttg atgcagccaa ttttttcggc
    31021 attcccatca tcatatcgct gtcgatgaac gcgacgattg ggatatcatg aggatcaaca
    31081 caaacaaatt ttcggtgatt tacttcatct gtgatgacgt agttgatcgt tacttctgct
    31141 gctgttccag aagtcgttgg tatagctaat ataggtaaac atggattttt cgtatcagca
    31201 acaccttcta aactaatgac gtctgaaaac tctggattcg tcaaaataat cccaatggct
    31261 tttgccgtat cgattggcga acctccaccg attgcgatca aacagtctgc ctctgcctct
    31321 ttaacaaaag ccactccatc ttttacattt tggatcgttg gattaggtac gatcccgtcg
    31381 tataaagcat aagcgattgc tgctttatct agaagttctg ttacttttgt tgctataccg
    31441 tgctcgatca atcctttgtc agtaatgaca gctgctttgc cgaatcctcg ctgctgaaat
    31501 tcagggacga tcgactcaat cgctcctttt ccaaaatacg atgtttcatt taaaatcatt
    31561 cgattactca ttttctattc ctcctaattt gtattatttt tcacaataaa aacgaaagaa
    31621 ccccaagcaa ccggttgccc agttgcttgt tgctacagtt caaagactgg catcaattcc
    31681 caactgactg gagaggcatc tggatttgtc tccatgatat cttccatgta cttccaccat
    31741 ttttgattga tagatgtatt tgccattttt tcccataact tttcatcttc gatcactaag
    31801 taaccaaata acaaacttgt actcggatca agatagatcg aatagtcgat gaccccgtgt
    31861 tcacgaagtg ctgtacgcat ttctggccaa agttcatcat gtcttttttt gtattcttct
    31921 tgtttttctg ggtaaacttt catgcaaaaa gcttttttca ccatgtcaga cacctgcttt
    31981 cgtatgaact tcctccaaat aacctgcttt tggagtgaca ccaaacgcat ctgctaaatg
    32041 ccacaggttt tcgtccgtga ttgtttgcaa gattggtcct tgcgctttga cgtacgtata
    32101 gacttctgct gccttttctg ctgtctcgat caaaccaaag acttcatcca tgtcttttcc
    32161 tgacccgtag attccatgtt gtggccataa aaccaatcga gtttctttca ttttttcagc
    32221 tgttgcttcc ccaatctcat ttgttcccgg aaccatccac ggaatgatgg aaattccttc
    32281 tggaaaaaca actaagcatt ccgtacacat ttgccatagc gtacgactaa aggaacgttc
    32341 gtctaattca tgcgtaaaac tcatcgctaa tagatgacta gcatggcaat gcataacgat
    32401 ccgattatct gggtctactt ctaaacgggc gatatgactc atgaaatgac ttggcaactc
    32461 gcttgtagga aggcattcgt tttccagccc ccacaaaagt tccagtgttt tgccatcttc
    32521 agcaactcga acgattccaa gattctcggc aggttcgctc gctacatttt taaaatatct
    32581 tcctgaacca gttacaataa aatagcatcc tgctaaacgg ctggcatcaa aaatcatcga
    32641 gatgcggcgg atcacttttt caggatccaa gaacggtgtg atctcttctt cttttagtaa
    32701 ataagaaata ttcccgccat ttctttcatc ccatcccaag cgataaagat tcgacgtcgt
    32761 ttcgatcatt tctttcacat aaggcgcttg taaaatatct atttttttca atatagccac
    32821 actcctattt tcttaattcc aacacgtctt tttgatattc ctggacttct tccagccaat
    32881 ccaaaccaac tggaacttga tttttgtaac agaaataatt ccatacatcg gcatacggga
    32941 aatctttcaa ttcttccgta taagccattc ttgaagtaaa gtcaaaagcc agctccattt
    33001 ctttcaatcg ctcaactggt tccagcatag ctttcagcag tgctttttgt gtatttctcg
    33061 taccgatgac ccaagcagcg atacggttga tcgttgcatc aaagaaatcc agaccgatat
    33121 ttgttttttc caacaattcg ttacggacta attctttagc gatttcctgc aattcatcat
    33181 ccatcaaaac cacatggtct gaatcccaac gcactggacg ggaaacatgc aacatgattc
    33241 ctttgctgaa taaagctaat gaagataatt tattggaaat cacttctgtt ggatgaaaat
    33301 gtccagcatc tagacaaaca agtttgtcac gagtcaaccc gtaacccata taaaattcat
    33361 gggaaccgac tgtataagct tcagcgccta aaccaaatag tttgctttct actgcatctt
    33421 gtacataccg ttcatctaac tcttctgtaa aaatttcatc gagtgattcc attaaacgtt
    33481 cccgtggggt aaaacgatca atcggattat ctttcatccc atcaggtacc caaaagttat
    33541 tgatacttgt ttgcccagtt tctttcccaa agtactccgc gattttgcgg cttcttttcc
    33601 cgtgttcgat ccagtattca cgaacttctt tatctggatg agccaatgta aagccgtctt
    33661 tcatcattgg atgcgaaaag aaagtcggat tgaagtcaag accgatttct tctttttttg
    33721 cccactctac ccatttttca aaatgtttcg gctcaatttc atttagatcg acagattctt
    33781 ctgtatccaa atagatagca tgaaggttca atttgtgttt gcccggtatc aacgaataag
    33841 ccttctccag atcttgtcta agctcttcag gtgttcgtgc ggctcctgga aaattccctg
    33901 tcgccatgat tccaccggtc aattcgccat ccggtgtcaa gaaacctttg acatcatctc
    33961 cttgccagca atgcatagaa attttgatgt cctgtatctt ttctagtaca gcatctgtat
    34021 ctacaccgat agttgcatat ttttcttttg cttgttcgta atttctttca atcgtgttca
    34081 tacgattact cctttgattt tattcacgta ccattccatc gtgttttgct tattttcatc
    34141 tgggtaaaaa gtttcacatg gaaacgactg tttgattaca tttcttgctt gttctatatt
    34201 cgaaaactga cctgtactga gcatttgcat ggcaacattc ccgattgctg tggcttcact
    34261 aggtcctgct tctacaatga ttccggcata atctgctgtc aattgattca gtagacgatt
    34321 attcgatccg ccccctacga tgtgcagctt ttccaatttc gttccagtta agtgttctac
    34381 tttgatcagc tcttgtgcgt aacaaagagc aagattatca tacacacaac gagcgatagc
    34441 tcccgatgag acagggactg gctgctctgt ttcaaaacag tattgctgaa tctcttctat
    34501 catatttggg ggattcaaaa atcgagggtc attgatttcc atcacttgct gcagcggttt
    34561 ttcttttgca gcaagctctg ccaattccgc ataactgtac tcgtactctt gcattcgggc
    34621 aacttcctgg atcaaccaca tccccatgat gtttttcaaa aaacgcaccg tgttatgggc
    34681 accccattca ttcgtataat tttcagaaaa agcattgcga gaaacattgg cgatctttgt
    34741 ttcaatccct aaaagcgacc atgtcccact gctgatataa ccccaacgtt ctccttgacc
    34801 tggcgttcct aatacagctg acgccgtatc atgacttgct actgtgataa aggtcacatc
    34861 tggcaaatca tattttgaaa attgttcttt ttgcagatct cctagtatcg ttcccggctc
    34921 tgtcaatggt ggaaataatt ccttcgagac accgattttt tcaagaagtt ctatttccca
    34981 ttcttttgtc cctgcattca gcagctgcat agtagatgca ttagttttct ccgtcaccat
    35041 gttaccagta aagacatacc ctaaataatc aggtatcagt aacaatttat gtgcttttgc
    35101 caaattttct ttttcttcag caaacaattg gaacaaggta ttgaaaggct ggatctgaat
    35161 ccctgtctgc tcatacaact tatctagaga atagatttca gaaaactgtt ctactgctcc
    35221 attggtacgc ttgtcccgat aagcaatcgg atcatttatc cgatcccctt gttcatcaat
    35281 cagacagtaa tcgactgccc atgtatcgat ccccgcaaaa aggcgatcaa tccctgtctg
    35341 ctttaatttt tcaaatccaa tcaaaatctc tttgataaga taatcgatct tccagcgatc
    35401 gtgaccgtct ttgttccgaa agccgttttt gaaccggtgt acttcttcca agatcaaaga
    35461 accttttgca tccctcgtac ttttcattag tcgtccgctt gatgcaccga tatcgatggc
    35521 aacaaatgtt ggattcattt ctccacctct tttactttat tgttgattag cactttatta
    35581 ataaagcgtt atcatttatt gacaatgcta tcataataca gaatcttata ttttgcttca
    35641 cactattttg gtatataatt gcattatctt gacaagttgg gagtgtttac atggattact
    35701 gggaaaaagc caaccactca tggtcggaag actcaaaaag agtgatcttg acgcctacac
    35761 aaagaagcag gaatttattt ttttatatcc aagaaatcgg ttacttcaaa gcaccaatcc
    35821 cttactatac tgaacgagca catttacctt cttacttgat aaagtttact ttgtctggca
    35881 aaggttcgct tacatataat gataatcagc aacagctaaa agctggagac ttatttttta
    35941 ttgactgcca agaatatcag cgttatcaaa cgatcagcga agaaccatgg gaaatggatt
    36001 ggatccattt gaatggtcag aacgtccaat cattttatca ggagttcaca aaagacggcc
    36061 ataacacctt ccattcaaca ggagatccat cagaaaacaa gatccaccat ttgatcacag
    36121 aattgatcca catgcaacaa ggcacgaatg cccgtaccga tttccagtcc tctgtagcta
    36181 tccacgagtt gctgaatgaa ctgattttgc aaaagtatca gctagatttc gaagaagagg
    36241 atattcccgc ctacgtttac aaactgaagc aatacttaga cgaacatttc aaagaaacga
    36301 ttacacttga tcaattagaa caacaattct tattgaataa atatcagctg agcaaagatt
    36361 tttccaaatt tatcggttct ccaccaatcg attatttgat cagcaaaaaa ctttcttacg
    36421 ctaaagacct tcttcggtat acagaattta cggttcgaat gatttccgaa gaaataggga
    36481 ttgagaattt tgcttacttt agccgcctgt tcaaagcaaa aacaggacta tcgccgagag
    36541 actttcgtaa attcagctga ttcatataat atgtacgtca ttttttcaaa atattttgaa
    36601 atatatagat aaaaataaaa taaccgcagt ttctgcttct atgtaaacaa cagcagctac
    36661 tgcggttatt ttttagtgat cattctcatt gttcatcttc aaaatgttca taatgaacca
    36721 actgtttctg tgtatcacgt ttcaaaaatt ctttaggcgt tatgccgaac tctctcttga
    36781 atgctttcag aaaatgcgga taagaagaaa aaccactttt actatataca gcactagagc
    36841 tgcggtattc tcgcaacaag tgcttggcat agatcagctt tttcttcatt acatactgat
    36901 gaaacgtaaa tccagtgtac tttttaaatt ctcgtgtgat atggtatttg ctgacaaaaa
    36961 actgcttttc catttcctct aaagaaagcg gatgagataa attttttgag atatattgta
    37021 acatttgttc cacacgctca ttaggaacta cagcattctg ttcttgcgca ctctcttctt
    37081 tttgtacaac ttgattgaga aaaatcaagt attctaccag agcttgttca tattttatgt
    37141 cttcaccgta aattttttta tcaggaggat ggtcgataaa tgataatttt tgcttcaata
    37201 cttcctgttc tatttgcaat acacgactcc tcttctctcc cattggttga aaacaagccg
    37261 atagattaga ccatggcgtg gaacgacttt ctagaaaaga aggggtgaca aagatataga
    37321 cacgctcgaa tacatctgaa gattggcgga cgatacgatg caaatcattg gaatggatga
    37381 gcagcacact tcctgctttg atcgtaaatt gtcgtccgtt caaataaaaa gtcgcttctc
    37441 cctttaatgt agcatggatc tcatagaaat catgatgatg atagatcgtt ttattttggc
    37501 tgacgacatc tttgacatga tagatttcat actgatcaga aaccatcgta ttgacttgtt
    37561 caccgctgat tttcttttgg agcatgtgcc gcacctcctt ttgttcactt atgcacaatt
    37621 ttattatacc acgttttttg taatcaaaaa gagctgcttt tatatactct tataaaaaag
    37681 aagagacgtg gcatcttcta gccccgcccc ctccattcta aatattagtt aaccaacgtc
    37741 ttctttacat tattcaagaa gacttcttcc ataacgaatc ccatcatctt ttgtttcttc
    37801 aagaaccaca tacaggtacg gacgattctt catgatgatt tctttgattt ttgtgtactc
    37861 gatcacaccg tctcccaaat ttaccggcac gattttttcc tgttcgacac gaaaatcttt
    37921 caaatgaacc gcacatatct tttcaccaaa acgttcaaaa gcttcttcta ccaattggac
    37981 ttgttcttga taatcactac ttgtgatcaa attggtcgga tctaaaatga ttcctagata
    38041 gtccgaatct actgcttgga tcagctgctc tacccttgct aaagaataca ccggatgatt
    38101 caaacctggt tctatcccca ccatcatttg gtatttttct ccagctttta ccaagcgacg
    38161 gataacggac acagcttcag caaatgcttc atctgtgaaa ttttcttccg tatattggat
    38221 ttccggcaaa acacatccag tttccgaagc aaccatcgac gcaccaaagg aagaagcata
    38281 gcgaagatac tgctcgaact ttgtcagcaa ctcttgtcgt atcgttaaat ctggatgaat
    38341 catattgata tagcaactta aaattcctac ggtcacttct tgcttttcta aaactcgttt
    38401 gacataattt cccatcccag aattgatctc atttgcgcca gaaggtattt caggaaacga
    38461 aagacctaag gcgagctgca gtgtatgaat cccttgttct tttgtttttt ctgcaagatc
    38521 ttctatcgtt tggacttcgg aaagatcatg tcctctaatt gtaatgttca acataactat
    38581 tctcctaact cagcatattg ggtagccaca tgacgatttg cgggaagtaa gtaatcaaaa
    38641 acaagacgac taagataacc ccataaaaag gaagaagcgg tttcagcatt gcctctgctt
    38701 ttacgcctcc tacacttgca ccgacatata gtcctgtacc tactggcggc gtgatggagc
    38761 ctacacacag attcatgatt gaaaacaacc cgtaatgtac aggatcaacc cctacacttg
    38821 tgacgattgg taaaaatatt ggtgtaaaaa tcaagatagc aggcgccaca tccataaaca
    38881 tacccaccag taataacaca agattgacta ataacagaat aataaattta ttatccgtta
    38941 cgcccataat caacgaacta attgcctgag gaataccggt aaacgccatg gcgaaagaca
    39001 tcatggaaga agtagccaat aagaacatga tcgtccctga cattgctact gattgaaaca
    39061 acatattagg tatatctttt agttttaccg ttttatagta aaacatagca atcaacaggg
    39121 agtaaatgac agctatcgct gaagcttcta ctgctgtaaa tatccccgtc aagataccac
    39181 cgataatgat gacaatcaat agaaggctag gaatcgcctc ttttattatt tttccagcat
    39241 gagccatctg tcctttttcg acaacagggt atttattctt tttagcaacg atgaatgcta
    39301 ctagcatgat tcctagcgcc cacaaagcgc ctacgagata acctcccata aacaaagcag
    39361 agatcgaagc accaccagca acaagtgaat acatgataaa agcagtactt ggaggaatga
    39421 ccatacctgt cggagctgac gcgatattga ccgctgtcgc aaacttccgg tcatatcctt
    39481 cttccacttg ctgagggatc aacactccgc caatcgcggt agaagcggca atggccgagc
    39541 tagagatgga accaaataaa gcattcccta acacattcgt atgagccaaa gcccccggca
    39601 cgcgcccgac gaacaacatc gcaaaatcca ctagtttctt agctatccct ccattattca
    39661 tgataatgcc cgagagaacg aaaaacggaa tagcgatcag cgaaaaacta ttgatacttc
    39721 ccaccatttt ttgagctgaa ctaaaagcag aaacatcaaa cggtataact aacaataaag
    39781 tcatcatcga tgagaatgca atacttatag caatcggcat ccctaataca agcaacacaa
    39841 caaatacaaa aaataacact aatccggctt ctattgccat tttattcctc catttttata
    39901 ttttttcttg aattgctggc ttgttgctta atgtatcgat tagattgatc aggctataga
    39961 taatgatgag tacccccgat acaggtactg acagataaac atatcccata ggaatactta
    40021 aagaaggcga gatctgcccc attgtcaatg tgacagcatt ccatccgcca tacaccatga
    40081 tcatcgcagc aaataccaga aataaacttt gaacaacaac ttctaaaatt ttactttttt
    40141 tgccagttaa tttttcactt aaaaccgtta cggctaaatg tgctttttgt cccacaacat
    40201 acgcggaaga caacatggca aaccagacaa gaccgaatcg aacaagctct tccgtgaccg
    40261 tatttggatt tcctaaaacc gttctggaaa atacttgcca taaaacaaca gcgaccatcc
    40321 caacaagcaa aacagctccc aggatctcta ataccttgtt taaaatcatt ttcatcacag
    40381 ctacgctcct tgctcttgga tcttttgata aaggtctttg taggtaggat cgtttttgaa
    40441 tatatcatga agtggggcaa ctgcctctcg aaacggtgta ttatctacat cattgaattg
    40501 gactccatgt tcttcttgtg ctaaagcttt ttcttcttca attgcttttg caaaagcttc
    40561 aatttcaaaa accgttgatt ctttcgctgc ttcttcaatg gctttttcct gctcatcagt
    40621 tagctgttct ttgatggcat cattcatgat gatgatatct ggcacacgtg tatgctcatc
    40681 ataggaatag tatttagcca caccaccatg tccagctgta tacaagacaa attcattgtt
    40741 ttctgatccg ttgatcaaat tggattgaag agacgtgtac acttcatcac ttcccatagg
    40801 tacgggcgaa ccgcctaaaa gctctaccat tttgatagcg gtttcgcttt gcatcacacg
    40861 gatcttcttt cccttcaaat cttccggcgt atggatcgga ccgtctacca tataaaagct
    40921 gcgctgacca gaatcataat aggtcaatcc ggtaaatcct aaatttttgg tcgattggta
    40981 gattggctcc attatttctt cattatccat caccttaaag aaatgttctt catcattgaa
    41041 tagataagga actgaaaaaa tcgaataatc tttagagaaa ctttccaacg ctgaggcact
    41101 tacttttgtg aaatctactg ctcctgactg tgtcaattcg atcagttcac gttctccacc
    41161 cagttgtcca tctgggaaat actggatctg tacggaacca tctgtttttt cttttactaa
    41221 acgcccaaat tcattcattg catcaattac tggctggctg ttgcttgcat aagcaaagcg
    41281 caaggtaact atctcattta catctgcatc tgcgacagtt gccacactag aattatttcc
    41341 gcagccgctt agacctagca gacctaggct aagcgaactt gctgcaaaca gcagtgatgt
    41401 ttttttcata tgggtttcct ttcttttaac aataagagac gagcaattct gtcaaacaaa
    41461 gcaccgttaa cgactgtcca taaggcattg ctgtgattcc gatattccga taaaaatcta
    41521 aactgtcccc cattcctgtc ccgattgaaa cgttttgaac ttctcctttt tcatcgattt
    41581 gttcaagcaa tccttggata gcacgataag cgacttcttt atattcttgt ggtaaataac
    41641 gtttatggac cgctttcaaa ataccgtaag caaatcctgc agttgcagaa gattctacgt
    41701 aagaagaagg atcatccaac aacgtatgcc ataaaccgct ttcagactga taagttttta
    41761 atgcacgcac ttgtgcttca agtgtttcga tcaataattt gcgcaatgca tcttctttcg
    41821 ataattctaa gatttcaatg atctctggaa tagcaatcgt caaccagcag tttccacgag
    41881 cccaaaatgc ttcggcatag ttatgatttc cttcaaacgt ccagccgtga taccacaatc
    41941 ctgttcgttt atccatcaag tatttgatat ggatcataaa ctggtaacga gcttcttcta
    42001 aatattctgg gcgattcaac agtttcccga tttttgccaa aggaagcact gtcatcatca
    42061 atgtatcgtc ccacaactga ttcttatttt ccggtccata tgtggcatgc tgtaacccgt
    42121 cttcatttgt ccgaggcatg tcattcatga cccattctgc ccattgttct aaataaggca
    42181 aatagcgtga atctttcgtg tcttcataca agtaagccat agtcaatagc ggtgccattg
    42241 tattgacgtt tttgggcgga gcgccttctt ccatacgatc ctcaaaccat tcattgacca
    42301 cttgataagc gcgaggattt tttgtcatct ggtaattttt ataaatacca tatagaccga
    42361 caccttgtgg ccaattccac actgtccagc ttttgtcatc tactttcagt ccatcaaagt
    42421 ccaacaaaaa ttcaccatta tcgtctttga tcgttgtcag gttttcaacg agcaggtcaa
    42481 ttttttcttt cactaattct ttaggtacaa aaatctcttc tttaataatc atctttacgc
    42541 caccttttta aacgctttca ttttatatcc ttaatatagc agtaataccg tccactataa
    42601 atatcgcttt ttgaacaaaa actgtctaaa attgacctaa atgttaaata acaaaagtaa
    42661 ctttcagtta cttggaaatt aaaaaaagaa cttggagaaa caacgttttc caagttcttt
    42721 ttcatctatt ttcttatctt ttttctacgc tttcttttgc ttatccgttg taagcatcaa
    42781 tgatgatctg tttcaattca gagatcaatg gttctttcgg attagctgtc gtacattggt
    42841 cttcgtaagc taattcagcc atacggtcta cggttgtatc tagtgtttct tgtgtgactc
    42901 cttgagcttt tagactcatg ttgatgccga cagattttcc taattcataa acagccgttg
    42961 ctaaagcttc gactaattcc tctgttgttt cgcctttcag acctaagaat tttgcgatat
    43021 ctgcgtaatc tgtatctgca cggaaatagt catatttagg gaacatcgca tgtttttgtg
    43081 gatcttttgc attatagcgg ataatatgcg gtaaaagaat cgcatttgtc cgtccatgag
    43141 ggatcccgta ttccccgccg attttatgtg cgactgagtg acaaatacct aggaatgcgt
    43201 tagcaaacgc cataccagcc attgtagaag cgttgtgcat tttttcacgt gattccatat
    43261 caggtgtttt tactgatttt tctaaatagt caaagactaa cttgattgct tgtaggctca
    43321 atccacgtgt ataatctgaa gccatgactg aaacatagga ttcgattgca tgtgttaaca
    43381 catccatccc ggtatctgct gtgacagatg ctggaactga catgacgaat tgaggatcga
    43441 cgattgcgac atctggcgtc aatgcataat ctgctaacgg atatttcaca tgtgtttcac
    43501 tgtcagtgat gaccgcaaat ggcgttactt cagaaccagt ccctgatgtt gtcgggatac
    43561 atacgaattg tgttttttct ggtttgtcga tcttatatgt ccgtttacgg atatccaaga
    43621 atttttgttt tgcaccaaag aaagatgtgt ctgggtgttc atagaacatc cacattccct
    43681 tagctgcgtc cattgcagaa ccgcctccaa gagcgattac tgtatctggc ttgaagtctg
    43741 ccatcagttt cgttcctgcg tatacggtat ttgtcgatgg gttcggttca acatctgaga
    43801 agacttcgat cttcacatca tttttacgtc gtgccaatac ttcacgaact gtatcgcagt
    43861 aaccgaattg gaccatacca gggtcacaaa cgatcatgac acgttcaacg ttttccattt
    43921 tctctaagta caatagagag ttcttttcaa agaagatttt tggcggtaat ttgaaccatt
    43981 gcatgttatt tctccgtttc gctacagttt tgacattgat taagtttaca gcagagacgt
    44041 tacgagaaac agagttctta ccataagaac cgcatcccaa agtcaatgac ggaatcattt
    44101 cgttatagat atcaccgatg ccgccttctg cagatggtgt attcactaaa atacgacaag
    44161 ctttcattcg aagaccgaat ttgacatgca actcttcgtt ttctgaatgg ataactgctg
    44221 tatgtcctaa tcctccaaga ttcaacatac cttcacacaa atcaaacgca tggtccgtgt
    44281 tgttagcttt gatcatggca agaactggag aaagtttttc tcttgatagc ggataatctg
    44341 ggcctacgcc ttccaattca gcaatcagca ttttcgttcc ttttggtaca ctgattcctg
    44401 ccaattttgc gatttccatc gcagaatgtc caacgattga tggattgacc gcatattttc
    44461 cttcgttcat cactgcatct tctaattttt gcaattcatc tggttttacg atataaactt
    44521 ggtgtgcttg gaattctgct tttactgctg catagatttc tttatccacg atcacagctt
    44581 gttcagaagc acaaatcatt ccattatcaa atgttttcga aacgatcaaa tcgttcactg
    44641 cacgtttaat ttttgctgtt ttttcaatat aagctggtac gttacctgga cctacaccta
    44701 aagctggttt tcctgtagag taagcagact tgaccattcc agctccacct gtcgctaaga
    44761 cgatcgcgat acctggatgg ttcattagca tagaagtcgc ttcgatcgaa ggatgttcga
    44821 tccattggat acaattttca ggagctccgg cagcgatagc agcatcacgt accactcgtg
    44881 ctgcttctgc agaagatttt tgggcacttg gatggaaagc aaaaacgatt ggatttcttg
    44941 ttttcaacgc gatcattgat ttgaaaatcg ttgtggaagt cggattcgtt gttggagtta
    45001 ctccgcaaac cacaccgact ggttcagcaa tttcaatcaa ccctttttgg acatcttcat
    45061 tgatcacacc aactgtttta tcatgtttga tattatgcca aatatattcg gacgcataca
    45121 tatttttgat tgctttatct tcgtaaacac cgcggcctgt ttcctctaca gccatcttcg
    45181 ctaatggcat atgttgatcc aaagctgcca ttgccatttc atgcacgata tggtctactt
    45241 tttcttgatc aaagctttcc atttctttca atgctgcatt tgctttttct gccaaatcat
    45301 caatcattgc ttgtacgtca attgtcttcg ttttttcttt ttctattact ttagccatca
    45361 ttatcctcct aatgggtcat ggttatttgt taaataattc acatgtatat catacaatat
    45421 ttatttttct atgtaaacac ttttttgtga tttttttcac agttattttt aaatataaaa
    45481 ttgctttatt tacaggtgtt tgtatagaaa aatatataaa gttatttatt attatttata
    45541 tgacacaccc gattattcaa tattgagcaa tatatcccat gctcttatct ctttcttttt
    45601 tgaataaaaa atgaggtagg acatctttct gccctacctc gtctttaaat aagctttgat
    45661 acttattttt cttccttctc ttctgtctct tccagcgttt ctgttgtttc agtttttact
    45721 tcaactgtct cagctggttc ttctgttgtt tcaacagtag tcgttgcatc atttgttacg
    45781 acagtccctg gtttgaccgt tttgattgct gcacgatcgt actcaaggaa gatcccttcg
    45841 cagtctaaaa caactgtccg tttgtcattg tcgatttctg aaacaactcc atgtaatccg
    45901 ccgatcgtta cgactgaatc acctggtttc atgctatcta acaagttttg acgttcttgc
    45961 tgttgtttct tttgcgaacg tgacataaaa aaccacattg ctaatagcgc gataaacatc
    46021 acaagcattg gtaatgttcc catttttttc acctcactaa ttctgtatct acaaaaaaca
    46081 ctttagcaga aaaatgctga taacactagt tttttactta atttttaaaa gattttcctt
    46141 tagaagcttt ttgcgttttc tttattgaac ccgtattctt caaaaaaggc ttctctaaat
    46201 tcaagcaaat tatcatccat gattgcttga cgtacttgtt tcattagatt caacaagaag
    46261 tataaattgt ggtaagaagt caaacgaatc ccaaatgttt catcacattt gatcaaatgg
    46321 cggatatagg cacgtgtata gtttttacat gtataacaat cacatttctc atcaattgga
    46381 cggaaatcat gggcatattt cgcatttttc actactagac ggccttgaga agtcatacat
    46441 gtgccatttc gagcgattcg agtaggcaag acacagtcaa acatgtctac tccacggata
    46501 acaccatcga tcaatgaatc agcagcaccg acacccatca agtaacgtgg tttgttttcg
    46561 gggatcaacg gtgtagtgaa ttctaataca cgattcattt ctgatttagg ttccccgaca
    46621 gataacccgc cgattgagta accagggaaa tccatcccga ctagatcttt cgcgctttga
    46681 cgacgaagat cttcaaaccc tgctccttgg atgattccaa ataatccttg acggtctgga
    46741 ttagcatgag ctttcaatcc tcgttctgcc catcgagacg tacgttctac tgattttttc
    46801 acgtagtcgt aactttcatc gaatggtgga cattcgtcaa aactcatcat gatatcagag
    46861 cccaatttat tttggatgtt gattgctttt tctggtgata aaaacatacg cgaaccgttc
    46921 aagtgatttt tgaaatgcac gccttcttct tcgatattcc gcatatcact taatgaaaaa
    46981 acttgaaagc cgcctgaatc tgttaaaatc ggttgatccc agttcataaa tttatgcagt
    47041 ccgccagctt cttctactaa gtcttcacca gggcgtagcc ataaatgata tgtattactt
    47101 aaaataacgc cagcacccat ttctttcaac tcttctgggg acattgtttt tactgtagct
    47161 aatgttccta ctggcatgaa cattggtgtg gggaaagttc cgtgtggcgt gatcaattcc
    47221 cctaaacgcg cgccagtatg tttttctttc ttgattaaac gatagcgaat ggcaggttgc
    47281 gtcattcatt tccctacttt ctatataaaa tatcttgaca actcccacca tcataatcgt
    47341 ttcaagtgct ttttgcaagt tccctcggct tccttctact ttatttaata ttcttttttt
    47401 tatccttttt ttatgaataa caaaaaaaag aaaaacatta tgacagaaat attttttgat
    47461 acatggtata ttggtgttat taaagaaaag gagggaaaaa tcatgcaatc ttctgcagaa
    47521 ttaaaacgcc aagcaaaaga gagtctaaaa ggacgttggg gacaagctgt tttactaaat
    47581 ctaatcccta cattgattgg tattgctact attcttctta ttatgattcc tattatcttg
    47641 tttgccacca ccatatatga tggatcagcg agcgtctcag aagcgacaac ttcaggaggc
    47701 ggtagttcag gaggcggtgg cggaattatt tccagcatca ttagcgcttt gttcatgagc
    47761 ggtatttctt ggacttatct tgatatttta cgtggacaaa aagatcagat cgaaccattc
    47821 aaagatgcat ttcgtggatt caaaggtttg tttatcggcg gagtgatcag cttagctgtg
    47881 ctgatctcga tctttacaac attatggggt ttgctattag ttattcctgg aatcattaaa
    47941 agctattctt attctcaaag ctattttatt tactacgata tcgtccaaga aacaggacaa
    48001 acacctaaag tactggatac gatcactgca agtcgaaaat tgatggatgg ttataaagga
    48061 caacttttct ggttagatct ttcatttatc ggctggcata tcttagcatt agcaactgct
    48121 ggtattggtt atctatggct cagcccatac atttctgcaa caaaagcagc attttatgaa
    48181 caattgccaa aagatattta aaagagagtg tgacttgtga ccgagggggt ggcaggagaa
    48241 caccgtttat cgagaggcag taaaagaaac attcagccca ttagcaaaaa ggaggtgtga
    48301 caacattcat gtcacacctc ctttttgtat agttccagta aaaagaaaat caacttagaa
    48361 acagttaatc attcctcttc ttctgcttcc ggtaatcgat ggaacttgat cagttcattc
    48421 accgcgattg gtacgataga taaaatccct accaagaacc agtgattcaa actgatgtgt
    48481 actaggccga atagttcttg cgtaaatggc agcaaagcaa cagccgttgt aatagctaat
    48541 gccagtaacg ccatttctac caaagcttta ttggtcgcta aattgacttt gaagatcgat
    48601 tggctgctgc gtacattgaa cacatgcaga atcgatgaat aagctaagat caagaatgca
    48661 acagtttgac cgacatgctg acttgcgtct acccatgggc tgatagtatc gacataacta
    48721 cctaaataat aaccaaacaa ggttacgatg gtgaagaccc ctgcatttat ggcaattttt
    48781 tgcaataaac ctcgagcaaa gatcccttca tttttcggaa tgggtggctg atccatgatg
    48841 ccgtgttccg ctggctctct tcctaaagca aatcctggaa gtccatcagc aactacattg
    48901 acaaacagta gttgcacagc tgtaaatggt gctccccatc ctaacagcat cgcaatcaaa
    48961 acaataaaga tctccgaaat attacagctt agcaaaaagt tgatagcttt acgaatattc
    49021 tgataaaccg aacgtccttg actgacagcg tctacgatcg tcgcaaagtt gtcatccgtc
    49081 aaaatcatat ctgcggcact ttgcgccaca tctgttcctg tgatccccat ggcacaccca
    49141 acgttacttg ctttcaacgc tggtgcatca ttcacaccat ccccagtcat cgctacgact
    49201 gcacccgtac gttgccaaga tttgacgatc cggatcttgt cttctggcgt tacccgagca
    49261 tagacagaga gagctttgac acgcgcatct aattcttcgt ctgataattg atgcagttct
    49321 gatcctgaaa gtgcttctga cggatcattc aagataccta attctttcgc tatggcactg
    49381 gcagtcacca catgatctcc agtgatcatg actgttttga tgccagcctt tttcgctcgc
    49441 ttgattgctc ctttactttc tggacgtggt ggatcgatca ttccgatcag accaatcaaa
    49501 cgtaagtctt tttccagtgc ttcagaagaa atatcttgcg gttcttcgtc ataagtagca
    49561 taaccaacag caatcactct cagtgcccgt ttaccaaagc gatcattaac gatcgccgct
    49621 tgttctacat cgcctgaact aaagcgcgga gcaagaacat cgaatgctcc tttggtgatt
    49681 gagatgtatt tttcgcccat ttggtgaact gttgtcatca tttttcgttc agaatcaaaa
    49741 ggaagctcgt ttactcgtgg atatttttct tctaattctg cctttgtatg ataattctct
    49801 tcaacagccc ttacgatggc tacttctgtt ggattacctt tgataacaag gtcgtcatct
    49861 tcttgctcga ccgtcacatc tgtacatagt gcagccattt tcaatatttc catcgcgctg
    49921 ttcgtcattg aatcttcgat attcgttact tcatcttctt tgctccatac ccggcggacg
    49981 cgcatttcat tttgtgtcag cgttcctgtt ttgtctgagc agataacatt agcggttcct
    50041 agcgtttcaa ctgctggcaa ctgtcggatg atcgcatgtt tcctcgccat cttctgcaca
    50101 ccgtatgcaa gtgtcaacgt aacaataaca gtcaaggttt ccggtacagc tgcaacagcc
    50161 aaggaaatcg aagtcatgaa catctcaagg agtggttctc cttgcagttc accaatcaca
    50221 aatactaaag ccgcagccgc taaagcgatc atactgattc gtttgcctag ctgattcaat
    50281 cggacctgta aaggtgtttt ttgcatagta ttttcgttca aaagatccgc gatcttaccc
    50341 atttctgtag ccatgccaat ggcagtgacg atcgctttcc cacgacctgc agcaaccgta
    50401 catcctttaa ataccatatt aagttggtca cctaatgact catcttcttc agcaataaat
    50461 gctgcatctt tttcaactgc ttcactttct ccagtcaacg ccgattcttc tacacgtaat
    50521 tgtgatgctt cgatcaaacg ggcatcggca gaaatagcat caccggactc taaaacaaga
    50581 atatcaccca ctaccagttg ctctgactca acagtggtca ctttgccgtc tcgtatgaca
    50641 gttgccgttt gtttattcat atcttgcaag gattccagtg ctttttctgc gtttccttct
    50701 tggacaatcg ccaataccga gttgatcaca acgatagcga tgatcagcag tccttcaaat
    50761 aaatccccat gttctgtagc aaatgcggta taaaaggaaa tcaccgctgc tacaagaagg
    50821 atgatcgttg taaaatcgga caagcttcga aagaattttt tgatcatcga ttctttcggc
    50881 gcctcatcga atttattcaa tccctgcttt tttcgccgtt catccacttc ttgtgaagac
    50941 aacccagtat ctggatctgc ttctgttacc ttctttactt cttctattgt ctgcttgtat
    51001 tcttccatcc tattcctcct ttaaaatctt cgtacaaaaa aaggtactta ttttactata
    51061 acatcctttt tttatttact tcaaataaag ggcttatcga ttatttgtta agaaagcgta
    51121 aaatttaaag tgcttatatt tgcaaacgct accaaaggat gctattatat agatgtaagg
    51181 aaggaggata ccatcaaata gacagaagaa tttctcgata gaacagtgta agcaaacaaa
    51241 gttgttagaa gtacaggttt cacccagaca tgcgtgtaga gtacaaacct ataaatagtg
    51301 aatgcgtatg gatgtttgga tcacgaagta tccgaaacaa gaagtaatgc gatacgtaac
    51361 aattgaataa cgtaaacgtc tgaaaattga ataggaacaa gagatgtttc aaatgttgaa
    51421 gatgtaacga aaacaaaatt tttgtttaac atgaagaaat tcaacaccgt gctttgcatg
    51481 tatgatggaa agttacaatc atttctgttt atttgaatcc ttccgaggta caagaaagcc
    51541 gagcttatcg agagctcggc tttttctttg tcataaaatc tttgtaaagc ttttttgtta
    51601 ttcaaaacta gcggcttttc ataaccttat ttcacgaaca tcgcatctcc aaaactaaag
    51661 aaacgatagc gctcttcaat cgcatgctgg taagcagata aagtcaattc tcttcctgca
    51721 aaggcactaa ctaacatgac taaagtcgat tttggcaaat ggaaattagt tgagaaagct
    51781 tctaccactt tgaattcata gccaggcgta atgaaaatat cagtccatcc gctgtctgct
    51841 ttgatttctc cattaaattt cgttccaatc gtttctaatg ttcgaattga agttgttccg
    51901 acagccacga ttttcccgcc atttttacgt acttggttca atcgctcagc cgcttcttcc
    51961 gtcaaacggt agaattcact atgcatatgg tgttcttcaa ttttatctac gctgacaggt
    52021 cggaatgtac ctaaccctac atgtagtgtc agatagacta attcaacacc ctttgcttcg
    52081 atctcatcaa gtaattcttt cgtaaaatgc aatccagctg ttggcgctgc agcagaacca
    52141 ttttctttcg catagacagt ttgatagcga tctgggtctt ccagacgttc tttgatatat
    52201 ggaggcaatg gcatttctcc caatgattca agaatctcta gaaaaattcc ttcatactca
    52261 aagtcaatga tccgtccgcc gtgttccaat tcttctttca ctactgcttg caatctgcca
    52321 tcaccaaaag agattcttgt cccaactttg gcacgtttcg caggtttgat caatgtctcc
    52381 cacgtatctc cttgtgtatt cgttagaaga aggacttcta aatgcgcacc agtatctggt
    52441 ttttctccat ataaacgagc aggcagaact cgtgtattat tcataactag cgcatcacca
    52501 ggctgtaact cttcaatgat ttcatggaaa tgtttatctt ccatctctcc agttttccga
    52561 tccaaaacaa gtaaacgaga atgatcgcgg tcttttaatg gtgtttgtgc gattaattct
    52621 tccggcaagt caaaatcaaa atcttctgtt gttaacatat cgtcactcac tttctatcat
    52681 gtgcttcatc attctatcat gtttttcgtt ctgttggcaa aatcagcact ttttcacttc
    52741 tattttttgt tttcctaaat aaaaaaacta ccctatttgt attttttcat tgttttaggc
    52801 atactagtaa tagatagttc ttcattgaaa atagaaaaag aggtggcacc atgattgaat
    52861 ttcaaaatgt gtcaaaaatt tataaaggtg gaaaagtcgc tgtagaggac gtcaatcttt
    52921 catttgaaaa gggtgagttc atttgtttta tcggtacgag cggaagcgga aaaacgacga
    52981 cgatgcggat gatcaaccga atgactgatc caacacaagg gaaaattttg attgatggaa
    53041 aatccatcca agaaattgat ccagtggaat tgcgacgtca gatcggctat gttatccaaa
    53101 gtattggatt gatgcctcac atgacgatcc gtgaaaatat cactcttgtc caaaaattgt
    53161 tgaaagtcag tcaagaagaa cggaataaaa cagctgaaaa aatgattgat cttgtggaat
    53221 tgccccgtga gatgttagat cgctacccac atgaattatc tggtggtcag cagcagcgta
    53281 ttggagtcgt tcgtgccctt gcagcgaatc aagatatcat cttgatggac gagccattcg
    53341 gtgcgcttga tccaatcact cgtgattcct tgcaagacct tgtcaaagat ttacaagagc
    53401 gtttgggaaa aacgatcgta tttgtaaccc atgacatgga tgaagcgatt aaactagcaa
    53461 gccgtatcgc catcatgagt gaaggccgtg tgatccaatt tgatacacca gaaaatattt
    53521 tacgtcatcc tgcaaacgat ttcgtagaag agttgattgg agaagatcgc ctactacaag
    53581 ccaagccaaa tgcgacaaga gtcggcgaag ttatgctaaa tacagcaatc acgattacac
    53641 cggaaaaatc attacaagaa gcaatcaaac tgatgaggga aaaacgtgta gatacgttgt
    53701 tagttaccga taattcgaat gtattaaaag gatttatcga tgtagaaacg ttgaataaaa
    53761 aacgaggaaa agtatccagt gtcggcgaca ttttgaataa acatgttttt tacgtaaaag
    53821 aatccgccta tcttcgtgat accttgcaac gcattttaaa acgcgggctg aaatacgtgc
    53881 cggtcgttga tgaacagaac aaagtcgtag gtatcctgac tcgtgcatca ttagtggata
    53941 tcgtctacga tgtgatttgg ggagaagatc ccgcaactac tgatgttgca gagtcagcta
    54001 ctcctgaaga atctaaggag gtctgaccta tgcagcagtt ttttcatcag cacggttcag
    54061 aaattctctc aaaaggtttt gaacatcttt atatttcagg acttgctttg atcattggta
    54121 tcatcattgc tgtaccgctt ggtgtcttac tgacacgttt tccaaaagct gccacgattg
    54181 tgattggctt aaccagtgcc ttgcagacaa tcccttctct tgctttattg gcattgatga
    54241 tcccgctatt tggcgtaggt aaattacctg cgattatcgc actctttatt tattcattat
    54301 tgccgatttt acgtaatacg tacattggga tgaaaaatgt cgattggaat tatcgggatg
    54361 tggcaaaagg catggggatg actgagttcc aatccatttt ttccgttgaa ttgcccattg
    54421 ctatgccaac gatcatggct ggtattcgtt tagctgccgt ttacgtgatt gcatgggcta
    54481 cacttgcctc ttatatcggc gctggtggtt taggcgattt gatttttagt ggactgaata
    54541 attaccaacc agatctcatt ttggcaggta cgatccctgt aacaatttta gcactattgg
    54601 ctgattggct tctaggtctc ttggaacacc ggctgacacc gatcgcactt agagaggaga
    54661 atgaagaatg aaacgtatac taaaaatgac catggttctt ttctctcttc ttatacttgc
    54721 gagttgttcc ttccctggtc ttgccagcaa tacagatgaa gacaccattt cgatcacagg
    54781 tggaatcact tcagaagcac agatcttagc aagtctagtt gccggaatgg ttgaacatta
    54841 tacagataaa aataccgcaa tcatcaacaa tctggctact acaactatca accatcaagc
    54901 gatgatgaac ggagatgctt caatttcagc ggctcgctac acaggaacag atttaacgac
    54961 tacattgaac cttcctcctg aaaaagatcc gaaaaaagcg tttgctaccg tgaaagatga
    55021 atttgagaaa agatacggtc aaacgtggtt cccgtcctat agttttgaaa atacgtatgt
    55081 ctttcttgtt cgaaaagaca ccgcacaaaa ataccatcta tctaaagtca gcgatctgaa
    55141 aaatgtagcg gatgaattag tagctggtgt agatacttct tggatcaatc gtaaaggaga
    55201 cggttatgac ggcttccaag aaacttatgg cttctccttc aattcgatat tacctatgca
    55261 gattggactt gtttatgacg cagtcgaagc aggaaaaatg gatattgttc ttggctactc
    55321 tacggatgga cggatcgcca gttatgatct agtcatgttg gaagatgatc gtcaattctt
    55381 cccaccatat gatgctgctc cagttgtaga caacaagata ttagctgata cgcctcattt
    55441 aaaagaagcc ttaaataagt taggtcatac catcagtacg gaaaaaatgc aggaattgaa
    55501 ctacgaagca gataataatc tagtggaacc atcagtagta gcacaacgtt tcttacaaga
    55561 aaaccactat tttgaaggga agtgaagcaa ttggataaaa tgaatgtctt tcaacaattt
    55621 ttctattatt tccaggaaaa tggctcctat atctttgcac aatttatccg tcacttcctg
    55681 atttctatct atggtgtcct ctttgcagca gttgtcggga ttccggttgg gatcatgatt
    55741 tctaggagac gtaaattagc aaactgggtg attcgtttgg cgaatatcat ccagacgatc
    55801 ccttctctag ccatgatctc cattttaatc attggattag gcctgggagt caatgtcgtg
    55861 attgttactg tatttttgta ttccctctta ccgatcatca agaatacgta taccgggatg
    55921 atccaagtag acaaaaacat ccttgatgtc ggcaaaggca tggggatgac tgctagacaa
    55981 cgactattca tggtagagtt accactctct gtttctgtta tcatggcagg cataagaaac
    56041 gcgttagttg ttgccatcgg gataaccgcg atcggtgcat tcgtcggtgc tggtggtctt
    56101 ggagatatca ttatccgagg aaccaatgca acagagggca catcgattat tttagcaggt
    56161 gcattgccaa ctgcattaat ggcgatcatc acggactggc ttttaggtat tttggaaaga
    56221 cggctagatc cagccagccg tacatcacgc tgatgttatt gtaaaaaaca aaagcttctg
    56281 cgaccatttg ttggtcgcaa aggctttttt agttctgtca tttttaaggc aataatccac
    56341 tctgattctt ccctccttat gggcaaagag ttgcttttct tagctattta tccattccgt
    56401 atactccaac ttcatactgc caacgggttc atatctgcat ccaccgacta agtttttttc
    56461 ttatccgcta aggcattttg ccaatccatt ttcccatcac tcgtcatgga attcatacca
    56521 catgatctct aaatactcgt ctgcttgttc tttgaattgg gcatcaattc ttacatcctt
    56581 tggcgtggct ttcatttaag ccttttccat cagtcctcga ttgttcgatg ccttcagctg
    56641 catcatcaaa actggtgact gccgtatcgg taacttcgat catcttttat acaatcggga
    56701 acttgccatc ctcatccttg gctaaaaaca tatctagcca ttccagcttc tcgccatggg
    56761 aggtttgcgg ttcctaaaac cggtacacga ttcttatgga ctgcatccgt cgcaactaga
    56821 cttggcgata cgatgatcct tcaccagaag agcacccccg taaaccaatt gatcgatata
    56881 ttgccaataa gaaaagacat tggcgtaaaa gatatcgatc cctcgtgatg cattgccgtt
    56941 gatactgcta ttcatggccg aaagcgccac gactttaact tcttccgttt gagtgacatt
    57001 catgttcggc aatgtcgctt tggatacccg ctctgctaaa ggaacgacac tggtattgta
    57061 tttcgtatct gtatcttttc caaaagacca tgccaacgaa tcctctggga accaataaga
    57121 cgactccggt tgattgtcca ttgctttttt caacatactt ttggctgttt ctcgataaac
    57181 gacatttcca attgtcttcg cctcactttg gcgataatcg atggtcgttg ccactgccaa
    57241 cactaacaac gatgctccaa ttttactgat tttccttgtc acacttcttc ctccttgttt
    57301 tacataaacc cgtcaacatt ccattcctaa actcaaacaa tgacccttca aaggtggaga
    57361 tataggaaca aattaaagaa atcatgcaat aaatccaaga aggttggttt tagaacaaaa
    57421 aaagagtctc ctttttgggg agactctttg ttaactatct aaaaaggttg gctcttctca
    57481 cttcactcat aatgtgtcca ggcagaataa atttctacaa ctttattttc ctcatcaact
    57541 ttgtaaacca ctcgatgctg tctattcagc ctgcgtgagt atctgccttc atgtgtaggt
    57601 ctcagctttt caaaagactg cgtgggcata tatggatctt ccttcaatgt ctgaatcact
    57661 tcttcaaatt gtttctttaa attcgtttgc ttgatttttc ttaaatccac tttcgcagag
    57721 tttttgattg cgactgtata gttactcatc aaagagcttc ccaatcaata tcatcgatat
    57781 tcgtaaagct actgtcgtct ttttcgcggt ctcgtacttt atctaaagta cctgtttgct
    57841 caagaaaaag ggtttcttga atcgctctcc agtcttccag cccaatcaat acagcattgt
    57901 ttccactacg atcactaata atctcgattt cagtatgatt ttcatttacc tctttcaata
    57961 actgataaaa atttttacga gcagtgctag gattcatcgt tgtattagcc attgtctctc
    58021 ctcctttttc tgatataatt atagcgtact tgattaagta cgtcaaaaaa atacaataaa
    58081 gcacttaaat tcagcaaact tttcctcttt tttacataga gaaaaagacc cctttttagg
    58141 gaggatcttt gtccacgctc acttcttact cctttacgac tagcggtagc caaacgtaaa
    58201 gtaattggta acaacgtatc atctttagca aaggtcgaat ccaaagcctg tgagtctatg
    58261 caacaacacc aatacacgaa ggttcctcgt tgcagtgtga cttgattgat atcttgaata
    58321 acctgaggat acgcacgaaa gatttgtaac ggttattcaa aactgatttt ggccgtcaag
    58381 tcttctactg cctcaaaaat gattgcctct ttcgtctcct gtcattcatg ttcactatcg
    58441 atttgatact tgcttagacc gttgggtttg tgaatcacca attaaaaatg gcactgagtt
    58501 atggtaacaa aaaatggtca cttgacgagg ctgagattcc tacttatgaa gacacatggc
    58561 acttaagagt cgaggtaaag gaagaagctg aaaatatagg aggctgtaca ttatttggca
    58621 aaggattcgg aaattatgat caagacatca aaatcaaaat gttttacagc taaataggaa
    58681 gaataataac atttcatacg ctgttcccat ctttaatggc ttatagaggg cacctttagc
    58741 tgagaataag acaaaacaga ccctctagct tcaattaaga gctagagggt ctgtttttga
    58801 ttttaaaact tggtttattt cttcaaatgg tagctatagg tgctgacaac gatattttca
    58861 cgccaagaca atcgcaatct taaccgcaaa cttgtttggt tatggagaat attctgccag
    58921 cgtctgtttg gtacaaattg aggaatcaac acagtagttg tatagttttg tttcgctgca
    58981 tttttactta cgatatctac ataacggata attggatttt caatcgagcg ataagaagag
    59041 tggacgattg aaaaacggac atctgggaaa tgctttttaa attctgcttc gatttctttt
    59101 tctttttcga catcttcgtc tagcgaaaca tgcatcgcaa cgacataatc accgatcgaa
    59161 cgtgcgtaat tcaacgcacc gatatttacc cgtgtcacat tcccgactaa tacaagaacg
    59221 gtattcccgt cataatcatg tagttttgca tcattttcta aacgaagctg ttctgctact
    59281 tttttataat gatcatggat cttataaaaa atgaacataa cgattggcat aatgatgaag
    59341 aaaggccaaa tgtcccctaa ccgatagaca aacaaaatcg caatgattgc atatgagatg
    59401 aacgcacccg taatattggc aatcgatttg cttaaccatc tttttccttc ttttttccat
    59461 ttgacgacca tccctgtttg agataaagca aatggaatga acacgccgat agagtataac
    59521 ggaatcaagc gttctgttga gccatggaaa ataaacagca atacgatcga acctagcgct
    59581 agtgtgataa tcccattgga atagcctagt cgatccccac gatcttgata catgtgaggc
    59641 atgaatttat cttttgccag attgtatgca agaactggaa aagcagaaaa accggtgtta
    59701 gcagcaactg ctaaaatcag cgctgtagca aattgaagga cgtaatacaa aataccatgt
    59761 ccaaagacag ctttaccaat ttgagacaaa acagtgactt ctttttcagg aacgatccca
    59821 taccaatagt tgataaatgt gatccccacg aagaagaaac ctaagatcaa tgccatcatc
    59881 gtcaatgtag cagcggcatt ttttgctctt ggcttcttaa agaatggtac agcattacta
    59941 atcgcctcca ccccagttaa agaggaagaa ccagaagaaa atgctcgtaa aatcaaagcg
    60001 atagaaacac ccggcacagc cgctcctgga agtgcagtag cttgcaatgg cacagcacct
    60061 gtcacgatat tatataaacc aacaacgatc agtaacgaga tcacaaggat aaatgtgtac
    60121 accggaaata ataggaacga tgcagattcc ctcagcccac gaagattcag catcatcagt
    60181 agcaaaacga ttgtcacaga aatagcgact tgatgaccgt agagtgctgg caccgctgat
    60241 gtgatcgctt cagcacctgc tgagacagat actgctactg tcaacatgta gtcgatcaat
    60301 aaagagccac cggaaatcaa gccagcattc tttccaagat tctcactgct gacgacatat
    60361 gctcccccgc catgaggata agcatggatg atttgacgat atgatagcgt taatgaaatc
    60421 aataaaataa taacaaaagc ggcaatgggt agcgaatacc agatggctgc agcagacaaa
    60481 gcgactaaaa cgacaacgat ttgttccgta ccatatgcaa tagatgataa cgcatcagac
    60541 gacaataatg ccaaagcagc aaatcgggtt aatttgtgct catcattttc agcagatttt
    60601 aaaggcttac ctactaacaa cctttttaag taatccaatt tctaaactcc tttacacaca
    60661 attttacttc aagttttata gtcgaaaatt tatagtaaac aagatgttat agtacgctca
    60721 ctgaccttaa aagtcaataa actttaagaa aataattgga aaaaaacaga aaaatatcaa
    60781 gcgctttttc aaacgcttta atacagcaaa aatctaacat ttttttatgg gctttttctt
    60841 tgaattctat aataaaagga tagaacttga cttagaaaat caagttctat cctttttgat
    60901 atcttaactt ttggaagaga gatgtaaggc tgctttctac ccatcattct tactactcga
    60961 agcatagcgc agttgtcaca acctcttgat aaacggtgtt taaaagcttg catctgcggt
    61021 aataagccca accaaattaa aaacataaga agctgttttt atttggtcgt tcttattact
    61081 tgatgcagag cagctttttt acaacctctt ggtacacggt gttcaatcag ctgaccctcg
    61141 gtattagctc aaaaaaacgg caaaatatga ggaactattt ttccattttt ttctctaata
    61201 ctcaggtcaa aacactgatt tcacaacctc tttttttaat gaaaaatatg gctctcatgc
    61261 gccgcttctt tgaatttcgt cattttttct tcgatgaacg gatagattgc cgttcctacg
    61321 attccaaaga caacaaacaa accgatcatg atccagattg ctttcgtagc atttggccaa
    61381 taaattcctc ctgcagattc ccgtaacaag tttaccgcat gagtaaatgg caagaacgga
    61441 ttgatcattt ggaagaattt ccctgagact tggatcggat agttcccgcc tccgccagag
    61501 atcgacagta ctaagatgat aatagcgatc cctttaccga cattatcgaa taatgcagcc
    61561 aagacataga cgatcatcat gaacgctaag gcgatcaaga cagcaaatga cacgctgtag
    61621 accggatttt ggacatctac tcctagtaag aaaatattcc ctaatgtaac gatcaagctt
    61681 tgcgcaatcg ccattgtcaa aaatgtcagc atacgagcaa cgaattgttc tcgtttcgta
    61741 aaattgtttc ggtctttctt tcctaaatag tattctgtcg tagcaacact tgaaagaaga
    61801 acagcgccga cccacagaca tagtgcagta taaaacgggg tgctagctga accgttattt
    61861 gcgattggat aaagcgcatt ggtttgtagc tcaacaggtt gtgtaaagaa gtcactttct
    61921 tttgttgcgt ctagtttcaa taatttgatg acttcgccta gatcgacttc ttgttcgcct
    61981 tttcggatcg cgtctgctgc tttttggata ccagctttga tatttggcca gtcattgttg
    62041 atcaagtccg cagcagcgtt cacagctgtt tcgacttctg gcattttttc atttactgtt
    62101 gtcaatgtac cggtgatttc attttttagt gctggccaat cattttggat aaaatcagcg
    62161 gccaatccta atttttgctg aatgatcggt aattcattgt tgtataggtc tgccccttca
    62221 ttgatgccat tgacgatggt atccatattt ccattcagca ttgtattggc atcatggatt
    62281 tcttgtccaa ttgcaggcat ttctttttga tatttttcta aaattgccac tgcattcgtt
    62341 acagttgctt tcgttgagct gagaagcgaa tcaaagtcga tctggtccgc tttgctcagt
    62401 gcgccttgtg cagtcccaat cgtatcgatg acttggtcta gtagagaagt gacagtggaa
    62461 ctgatttgac ctgtattgat accagcaatg atgccattga tattattaac aacggcatca
    62521 atttgatcta gcaattcatc aatagccttc gtgtcttcat tttgaacagc tgtgttcaaa
    62581 gcagataggc ggttttgcag attgcttagc aagggtttta attgatttag ctgatcaata
    62641 attggctgta atttcgtatt tccagcactt gtttgcaatt tagtcaggaa ctcgaccagt
    62701 ttatcgattg cattttgttg ttgagatatt ccgtcaataa agccgttcaa tacattgctg
    62761 atgtttttct tttcatcatc agtcagctta ccatcttcaa tagctgttct tacagtatct
    62821 attaacgaac ctgtcgttgt tgccacttgt ccaattgact tcaatgtaac atctacactg
    62881 tttgtgatgc ttggtatcgc atctttcagc tgagaggcac ctgattttgt ttggtttgct
    62941 agatcacttg cctgattttc taaatttttc acatctggca agatatcttg tacttgttgg
    63001 atgatcgtca agccttgttt tgcttcatcg attccttggt tcattgtctc ttggatcgaa
    63061 gcaaaatcgc cgtcgatctc tgctagttgc ttaccggcat tttggatctc tgggattttt
    63121 tcttgcaaat caaggatgat ttttgcttga tctttcaact caggcatttt gtcattcaat
    63181 gcaacgactt tttcacccat ctcatctact ttagggatgt aatccacgaa ttcatttgct
    63241 ttattcagtt tttccttgat ttcaggtagt tgactttgta attcgaggac ttgttttgtg
    63301 tagccgtcga tcgtgtccaa attttcatct gttgataaaa tcatatcttt aactttattg
    63361 atacttacta ggttcgtatc gatgtcgtaa cctatctcat tgaatacttt caataaagtc
    63421 gaactggctg ttttgataaa ttcatttgtg atttgcgatt ggatcgaact tgctccttta
    63481 tcggtgattt tcggtgcaat cgcattgatt ttttcattta ccgtatattc gatttttggt
    63541 ttcttgatat caccactagt aaaactcagc agatcttctg agaaatcctt tggcaagtaa
    63601 atacctgcgt aatactttcc ggaacggact ccgtcttcta attgttcttt tgaatcaacg
    63661 aattgccatc ctaattgttt atttttatga agcgattcga tgacttgctc cccaatggcc
    63721 acttctttac cttgaaactc tgctggcttg tctgcactgt aaacagcaat tggcagctct
    63781 ccagtatttc catatggatc ccataatgct ttgatgttaa accacgcata tagcgaaggg
    63841 atgatcatga tcgcgatgat cagaagtgtt gcaatcgggt ttttgaaaat ccgtttccag
    63901 tcaagcaaat ataatcgaag cgtatttttg attgactgca tagttctccc ccttcttctt
    63961 ttttcagctg aattacttta ccatgtattt catacttttt tcaatcggct tttttcaaaa
    64021 aagtcgaatt tttggacata tcacggcaat tgtctcattg taatctctta catattgaga
    64081 aataattaag gattacagcc tttactttat catgaaataa aagggaaaga taattttatg
    64141 ccaaagtttt tatctattat acgtatacgc aaaaaagcta aaacattttt tgtctttgcc
    64201 cctacaaaaa tgtttcagct tttttcgttt tttattcctg tatttcttat ttttcgaatg
    64261 acaggatgat tttcccagca ggaggatgtc cttcaactag ctgatgtgct tggatgacat
    64321 gcttaagtga agcagacaga ttcattgaaa taaaaacatg aaaggctcct ttttggtagg
    64381 ctttgattat gccagcaaag gcttctgcat ctagatattc ctttcttgga acaaacgatt
    64441 cataaaagcc ttcctttttt tgccgcaaat ctagatctgg gaggtcattt aatgcaacat
    64501 acctgccgcc tggtttcatg atctggatgc cggtttctcc tttgatactt cctttcgtcg
    64561 catcgattac aaggtctgcc tgatcagcaa attgcagacc tggatttgtt ttatcataag
    64621 cagcaaatgc tgaagctcct aattttttta ccttttcctc atttttactg gatgcactgg
    64681 ttaagattcg gattcccttt tcatggagaa gctggatcag acttgacccg accgcacctg
    64741 atgctcctag tatcattaca gtatctgtcg gctggatttc cgtcaaatgg ttgattagat
    64801 tataagccgt aattcctggc gtgaccattc cggctgcttc ttcccaactc attttatctg
    64861 gaatttttgc tacttttgct gatggcaaca cgacttcttc tccgtaagtc ccgccgactg
    64921 catggacagc tactcgatca cctacatgaa cagttgtgac atctgaacca atctctgtca
    64981 caataccagc gccatcatta cctggtacat aagggaattt caatgttcgt atctctttca
    65041 tttctcctaa acgcaaggca acatcataag gattcacact gaatgctttt atttctacac
    65101 gaacatgtcc agaaccgact tctctgggat gagcatcgat ttcttcaaaa acatcttgtg
    65161 cttctccgta accgttaatt gcaaatcttc gcatggtcac catctccctt tttctttaag
    65221 tttacgttta tgcctttatt ttgacaaaaa aaatgtcttt tcgttttggc gcatgattca
    65281 aaacaaaata gttgtctttt ttcgcgtaac gtgttaaatt atttatctaa aagcgtaaag
    65341 attacgattt acgctaataa aaaataattg gaggaatttg ttgatggcta aaaaatcaaa
    65401 gattgccaaa gcaaaaaaac agcaagaaac gattgaacgc tatgctgctt tacgccagga
    65461 attaaaagcg caaaaagatt acgaaggctt gcgtaaactt cctttagatg ctcgtccaga
    65521 acgattgaaa aatcgtgatt taatcgatgg acgccctcgc ggatacatgc gtaaattcgg
    65581 gatgtcccgt ataaaattcc gtgaattagc acataaaggt ttgattccag gagtaaaaaa
    65641 agccagctgg taatccaagt ctggctgtaa tgtaaaggag gagggcgcat ggaccactcc
    65701 ttggatcatc aaaaaagata cgttcgcttc ttctagcaga aatgctggta ctagaagcag
    65761 acgtatcttt ttttacgcac agctggtcct tttttatatg taaaggctgc atggttactt
    65821 cgctatattt atttagtaac aggagtcagt gcttcgatac ccacactaat tttcaatgct
    65881 cggtcaactt ctgccataat gggtcgatcc aaatggcaga ccttttcttt caatcttgat
    65941 ttatcgatcg ttctgatctg ctccaacagg ataacggagt ctctttctat tcctgttcca
    66001 gtagctttga tcccaatgtg agtcggaagc ttaggtttag ccatttttgc cgttatggcc
    66061 gcaacaatga tcgtcgggct aaaatggtta cctagattat tttgaataac caaaactggg
    66121 cgtgttccgc cttgctccga tcccacgact ggagaaagat cagcaaaata aatatctcct
    66181 ctttttacca taggatcctc cttattcttg atattctctt ggtatccgct gtcctaatcc
    66241 gcaagccact tcatagtgga tcgtacctaa ttgatctgct atatcctgca tagtgatttc
    66301 tttgtctgca tttttgccga taagcgtcac ttttgttccg acaggaaatt cttgaggcaa
    66361 tcggatcatc agttgatcca tacatacacg cccaatcgtt tcacagtagt tcccttctac
    66421 caatagagaa aaaccctgca tctttcgtgg ccagccatct gcataaccga tcggtatcgt
    66481 tccgatccac tctgcttcag gagtgatata tgtttctcca tagccgattc cttcacctgc
    66541 tgggagtttt ttgacttgaa tcaattcaga aaccagctca agtgctggct gcaatggata
    66601 aacttccggt agtgcgtgcc cagagggatt aagtccgtac attgctactc cataacgaat
    66661 catattgccg attgtttcat catgccataa agccgttgca ctattgctga catgtacata
    66721 tcgaggaagt tcgggaagtt ttttcagcac ttctataaag cgttcttttt gcagattcca
    66781 atatgtatca tctgcctgat ctgctgtaga aaaatgggta aataaacctt cccattctaa
    66841 tacacgactt tctttgatga atgcagctgc ctcttttact tcttcaggtg tacaaaatcc
    66901 gatacgaccc atcccagtgt ctgcttttag atgaatcgaa agaggcgtct ctagcttact
    66961 tttctctaaa acagcagctg ctgcttgcaa ccattcccgt gtacctgcag ttactgataa
    67021 atcatgagca acagctacag tcagtgcgtc gtaaggaacc atattcaaga taaggatggg
    67081 ctgagtaaat ccagcttctc tcaactcgac tccttcatct aaatttgaaa cacaaaagcc
    67141 cgaagctcca cctgcaactg ctgcctcagc tgtttcaata gctccatgac cgtatccgtt
    67201 cgcttttact actgcaaaca attcttttcc ttgcggaagt cttgcaactt catttgctac
    67261 attttcagtt atagcttttt tatgtatcac tgctttcgtt gggcggtgcc aagcgacaac
    67321 catcgttttt ccttctttct agtcttccaa aataatttgt gcaaaagctg tttcatttgt
    67381 atgagtaata ctgacccata cattgccttc atgaggtgaa cgcgtcacaa caggctgacc
    67441 attttcattt tttaaaatct cgacatcttg aaaggtaact tttccgatcc cagttcccca
    67501 agctttagag aaagcctctt tgcaagcgta acgcccgcct aaaaattcta cttggcgatg
    67561 aaaaggcaag gactggaata gtttcatttc atcagaggtc aagattcgtg caatgaattt
    67621 tggtttttct tcgatcagcc gagtgatcct aggaagttcg actgcatcga tcccaattcc
    67681 ttttatcata tgcttttctc ttttcaataa atttagaaca ctctcattct accaaattat
    67741 aaccattttg ccacaaataa gtccacaaat aacaaaaatg aaagtttctc ttaaaaaaga
    67801 gaaactttca ttttatgttt aatcgttatt tgaacggatt acaaatccgc gttttttatc
    67861 attgtgacgt ttaccgccat ctttttggct gttattattt ttgctataac cgccttttga
    67921 tttgtttcca cgataattac cgccgtcacg acgattacgg ttgtttccgc caccacgttt
    67981 gccattttta ttgtatcctt ttttctgcat tggcaatggg cgttctggtg tgatcttcac
    68041 tggcactgca tcagctggat cttttgctgt cgtttttagc aaaagtgcaa ctaaatcttg
    68101 tgcagaatat tcttctaata atttatctgc tgtttgcagg tatttatcta gaccattttc
    68161 tgcaagtttt gtttcgattt gttcaactgc agcacctaat tgacctttga atgcttcttt
    68221 ttcggttggt ggacgtaaag tcgtcatccg ttttttcgtc aagttttcaa tcacatgcaa
    68281 gtagtccatt tcatttggtg taacgaaagt cacagacata ccgccttttc cggcacggcc
    68341 tgtacgtccg atacggtgaa cgtagctttc tggatcttga gggatatcat agttgtacac
    68401 atgagtgaca cctgaaatat ctaatccacg tgcagcaacg tctgtcgcta caaggatatc
    68461 taaatgaccg ctcttaaatg aacgcaatac gctcattcgt ttttgttgag acaaatcgcc
    68521 atggattcct tctgcacggt aaccgcgtgc ttctaatccg cgtgccaatt cgtctacacg
    68581 gcgttttgta cgtccgaaga cgattgttaa ttcaggtgtt tgaacatcaa acaagcgagt
    68641 catgatatcg aatttttcgt attcttttgc gcggacatag tattgatcga tcaagtcagc
    68701 tgtcatttct ttcgctttga tcttcacatg atgtggattt ttcatgaatt taacgccaat
    68761 attcttgata gctggcggca tcgttgctga gaaaagtaat gtttgtcttt gatcaggaac
    68821 ttgtgaaatg attttttcga tatcttccaa gaagcccata ttcaacattt catctgcttc
    68881 atccaaaact aatgtttgaa cggttccaag tttcaatgtg tggcggttaa tgtggtcaag
    68941 catacgtcct ggtgtaccca caacgatatg tggacgatct ttcaagccac ggatctgacg
    69001 gccaatatct gctccgccgt aaacggcttg tacacgtact tttttgtcgc gccctaaacg
    69061 atataattct tcttgtgttt ggatagctaa ttcacgtgtt ggtgcgataa ctaaaccttg
    69121 taattcgtga cgatctggat cgattttttc taacattggt aatccaaatg cagctgtttt
    69181 ccctgtacct gtctgtgctt gtccaataac gtcttttcca gctaatgcta gtggaatcgt
    69241 ttctgcttgg ataggcgttg cttcttcaaa accagcgcgt tcgactgatt tcaacaattc
    69301 tggtgataat tctagttctt taaatttcaa atgaatcctc ctatatggtt gttaactgag
    69361 gatgtagctt ttttgctacc ccgtcgattt gttcggtcaa agtgtattgt ttctttttga
    69421 cagtggccac gcacgttttt atccattctt agcccctgtg gcaccaccga aaaatcactt
    69481 tgcgaagttt aggcattaga aaagccaaac gggtcgcgtt cagctttatc attattatga
    69541 catctggctt caacaagttg tttctatggc aaaaccagtc caatttctct tcatctttcg
    69601 gcaataggtt attctaacat agtttttcgt tttctgcaag tttacagatt tttatttcat
    69661 ttgctgaagt tgttctacca cttcaaataa gcccatgctg ttgctgcctt ttagcataat
    69721 actatctgtt gcttcaagat tttgtttgat agcatcaatc aatttattct tttctttttc
    69781 aaaatagaac acaggtagtt gggggtattt ttggttcaat tcttctttta gtgcatgcat
    69841 ttgttcccca taaaggaaaa tcatggcgaa tttctcagga tcgatatgtt cactcatttg
    69901 cgcatgcata tttaaagact ctttccctaa ttccagcata tctgctaaca cagcgattct
    69961 gcgtcctttt gttggcagat ttttaaaagt atctaatacc agtcccatgg ctgttggatt
    70021 tgcattgtag acatcgctca aaatagctgc accatttcct gccgttagcc attcggttct
    70081 gttttgagtg atagaaacat gggcgagccc ttggcgtatc tcaggtatgg acagtccaaa
    70141 gaagcgcccg atcgtatacg caatcaaagc attcgttaca ttataacttc caggcagcgg
    70201 gatcgtaaat gtttctcctt ctactacaaa atcggtttgt gcttttcctt ttgctgtcac
    70261 ttccgcatga acatcgccat ttccaatacc gaatgttgtg actgtttgta ctaatgttgc
    70321 cgttaaaggt gtcagcaacg gttcatctgc gggcacaacg agtaatccgt ctgatacaag
    70381 tccgtcagtg atttccattt tggctttagc gattccttct cgcgacccaa gattttctat
    70441 gtgtgcttca ccgatcattg tgattgctgc agcttttggt tgagccataa gtgaaagttc
    70501 cgtgatttca ttggcgtgat ccatgcccat ttccaaaacg atcttttctg tatcttgggg
    70561 catgtgcaaa atcgtgtaag gaagaccaat attattgttg taatttcctt gcgtcttata
    70621 tgttttgtat ttttcagaca agacagacgc ggttagatct ttcgtggtgg ttttaccatt
    70681 gcttcctgta attgcaacga cttctgcgcc tactttttcc aagtaatagg ttgccaattg
    70741 ctgcatggct ttcaaaggat ctgctacagg aataactgga aaattttttg gagcttgttc
    70801 aacaggcaag ctccaaaatg aagcagatgc tccgtttgtg atcgcttgct ggataaattc
    70861 atgtccatct cgcgtaccag caagaggaac gaataaactt ccttcggtta tcttccggct
    70921 gtcaaattcg acattggtaa tctttgtcag tgcagagcat tcgctgccca cggcttgtgc
    70981 aatttcttgt attgttagat tcattgagat ctctcctaaa aaaggttgtg aaaaaagcgt
    71041 ttggcctaaa cggcgggttc gcatgactct cacttccaag taaatttctc tattcgtatt
    71101 tagaagctag aatgaacgcg gttacgcaat cactccagcc tcttctttat ttctctttat
    71161 tcattttttt caaaaaaaga ttgacgctga tggtatcgat tcattcctaa ctggatcagt
    71221 tcttcaatca aatcaccgta tttcaagccc atattttccc ataagagtgg gtacatactg
    71281 aactccgtaa atcctggcat agagtttaat tcattcagga ataattcatt tttatttgtc
    71341 aaaaagaaat cgcaccggct caatccgctt ccacctaaca tcgtgtaagc taacttcgcg
    71401 tactcttgcg ctttttgata aacttcttct ggcacttcgg ctggaatctg catttcgatt
    71461 ttattattga tatattttgc ttcataatca tagaatgcta cgtcttttac gacttcacca
    71521 ggcaaagtcg tccgaacgtc ttcatttcct aatacagcaa cttcgatttc gcgcgcttca
    71581 attccttgtt caacgattgc tcgagaatca tactgatagg ctgttgctaa agcattttgc
    71641 agctcttctc ggttttctgc ttttgtaatg ccgacactag aacccatatt cgccggtttg
    71701 acaaacatcg gataaagcaa agaaccttca cattgatcaa atactttttt aggattttct
    71761 ttccattgat tcttaagtac tggtacataa ggaacttgcg gcacaccagc agcttgtaaa
    71821 atatacttgg tcatgatttt atccatggca catgcactgg tcaatacgcc tgcgccgaca
    71881 taaggcatat tcaatgtctc taagaagcct tggatcgttc catcttcccc gtttggccca
    71941 tgtaaaactg gaaaaacgat ggctccttct tctttgattt cgcccggatt gatcactttt
    72001 cctgtaaagc cttcctctgt ctgtccactt gggtcccatg aaagatgcaa gacatctttg
    72061 ctggcaggtt tttctgttag taatggacct ttgacccatt gtccttcttt tgtaataaat
    72121 acgagttgaa cttggtaata attataataa atggcattta aaactgaaaa tgcggaaaga
    72181 atggacactt catgctctgc gctgcgtccg ccatatagta aagtaatctt caaaagattg
    72241 tcctcctgta ttctaaatcg ttcagtgatt tactctcata ttttagcaca aaacaaaaat
    72301 aaacgaataa cttatttata aaaaaacgag ccagcttcta aaagaacgag cgtcatttat
    72361 tttttgtcag ctagattatt tgtcccgttt tttataaaaa gttatgataa agaaaaggag
    72421 ggatgatgat ggcgcaaaca aatccacgca gccgcttggc agaaatcatt cgagtgttca
    72481 tcaattacaa tgtagtgcca aactttgtac agcaaaaaaa tcccgaacag gtaaaaaaag
    72541 cctttgaaga actaggcccc accttcatca agataggaca gatgctttct gttcgagaag
    72601 atttgctttc ttctgccttc actcaaacat tcaaaacatt acaagatagt gtacctagcg
    72661 acacattttc aacggtcaaa aagacgatcg aaacagaact ttctctttca ttatcagata
    72721 tattcgatga tttttcaaaa tcgccttttg cctctgcttc tatggggcag gctcatcgtg
    72781 ccaagctgaa gaacggtgac gcggtagtcg ttaagatcca acaccctaac atcgccgaag
    72841 agatccgact tgatcttcaa ctatttgaac gagcgatccc tttgatcaaa tacattcctg
    72901 aaactagcgt agttgatcta aaaggggtac tccaagaagt aaaacgttcc ttgatcaatg
    72961 aaatggactt cttaaaagaa agtcaaaatg gcgaacaatt ctatcaaaag aacaatggct
    73021 ggaaagagat ccgcagcccc aagatttatg acgctttttg ttcgaaaaaa gtgatcgtga
    73081 tggaagaaat gtctggtaaa aacttgaatc atttaatgaa cgccgaaaac aaaactgaaa
    73141 cttttatcac tggaattcaa aacaagcagc tgaaacaaga agttgcaaag cttttagttg
    73201 agaactttat gaaacaggtg tttgatgatg gttttttcca tgctgatcct catccaggga
    73261 atctgctatt ccatgtacta acaaaagaag aacagacaca agctagccga aaaactgaaa
    73321 cagtccatga aaaagagttc ggttcctttg cttttcgagc ctccacttct gcagaagacc
    73381 ctgttgcgcc atataccatc aactacatcg attttggtat gatggggcat ctttctgcag
    73441 gacttaggca aaaactcaca caagccgttt tagcgctgta tacgaaagat gcttatcgaa
    73501 tcgaaaaagc ggtccttcga ctttgtcagc aagaaggctc gtttgatgaa agtcggttcc
    73561 accaagaact gacaagtttt cttgaacagt attatgattc tcctattgat gaaatcaact
    73621 tgcaagaagt attcacacat gtcgtgacga tttgtcatca aaataatctg cagttcgatc
    73681 gtgatatcac gcttttgctg aaagcatttg ggacattaga aggggtgatc cgcgtcctcg
    73741 atcctgaagt ttccctcatg gaagtcgctt cgccttttgc tcaacattat tttctcactc
    73801 atctggatgt agaagataca ttgaaacaaa gcggattaga cttattggaa gggatgaagg
    73861 ctgcgcctaa gatccctcaa cagctccacc atctattaga aatgtggact tcgggacaag
    73921 gaaaagtcaa tttggaattg aaaaagcaag acaaattatt gagcaggatc gaaagtatga
    73981 tcaatcggct tgttttcggt atgattttag ctgcattgat cgtagggtcc tccctgcttg
    74041 tccaagcagc acctgtagaa aacgcagagg tcgtttcctt attagggata tttacgtatg
    74101 ctattgccgc attcgtcatt atttttttgg caatcgatgc attgatccaa atgtataaga
    74161 agaggaaaaa gtagaaaaag gttgtgacag aaagaatcag cttcaagaaa taagaagggg
    74221 ttttacataa attgtttctc aaatttttgt gaaaattcgg cttatttttc gaagaggcta
    74281 cttctgaaac accgagaata aaaaatgtga aaaagctgtt ctgcacccag taataagaac
    74341 aatcaaaaag ggaacatgga acacatttat cctgtttccc tgttcccttt ttgatgtatt
    74401 cgagcataaa taaaatacct tcgttaccta ctatcttctg tacttggtaa accttagtca
    74461 attgcctttt ttaacgcttg acgaaaagac atatgatctt tctccgtaaa gggttttgga
    74521 ccctttgtcc ttatgccagc ttttcttaac tttccactat gataacgttg ttctagaagc
    74581 gtgccgatat tcgcttcgtc aaattctttt ccagagtgat ggaacactcg cacacccttc
    74641 ggcaatacaa gtgaagctaa accataatct tgcgtgatca aaaagtcatt cttcttaacc
    74701 ataccgacga ttttataatc cgcactatca gctccgcggt ctacgtagac gatttggaca
    74761 tgattagcgt attctttggt cgtataatgg tccacactgg tcacgataac cacgtcaaga
    74821 ccataggatt ctcctaattt tatgacatct tcttttactg gagaaccatc tccatcaatg
    74881 aatagcctca ttctttttcg ccttcttcca taaatgataa acagttacta atccgctcag
    74941 taaaagacca acattgatcc attttgccaa gataagcagt tgtacacttt ctatcggcac
    75001 tgattcatgg ttcagcgtga tcaataatag atggattggc tctcccattt cttttgctaa
    75061 aacaaacaaa agcgtcatca ataaaaactg gtggatcaca ctccctttat tttgagtagt
    75121 atacttgaca acgatccggc gatccattgg aatagctgta tcagtttgat aatattcctt
    75181 atttagccag ctgcctgtta ctacgtgcca gaaactaagc agaaagacac ctatcgcaaa
    75241 accatttgga atcaagagga ccaataaaga agaactcagt ccaagcaatt gcatagccaa
    75301 tggagaccat gttaaacgtt tcaaaatcca tggtccgact atttttgctg caatcaagcc
    75361 tgctgcataa ggcaaataca taaaaatcga aagtttttct aatccgtaca gtatgccaac
    75421 ataaaaactg ctaaataaaa aaaggaaatt ccctagcatt ccatttagaa aagtaaacag
    75481 atttagccag cttggcaact tccattcctt cttctggtgt cctaaaacaa acacgaacaa
    75541 gatgaacagc agcaagaaac cataaatagc aagatcaaaa agcctttcat cgaataacaa
    75601 tcgggcattt ctcaaaagaa acaataagcc aaagaatatc aaaaataaaa caagctctcg
    75661 cgtagctacc agatttcttt tgatgtcttt gaaatccagt tcgtaacgcg gataatgact
    75721 aaccgtatgg taggccatga cgtaaaaaag tgtataaata gtaaagcttg ctgcaatctg
    75781 agtcggtgca ggcaactcaa tcgcttgata caggagaaat agccagatag ctgcaaataa
    75841 gtactttttc ccatccatat tgataaaacc ttgcgtcttt tcgtgaaaat tgaccgtggt
    75901 attagcaggt acaagccatg ctgcactcag ccctagcaaa acacttgaaa aataaaataa
    75961 aggaaaataa aatacgccta atattccaag caatgaacca gcaccaccaa tcagcagagc
    76021 gatcatcagc aacgtgtaac tatctagacc aaatcgtaga ctgttgacta aaaatagtcc
    76081 agtcatccga aaggcataaa acagcgtaaa tggcaaaatc gtcagccaat tatgcgagtc
    76141 tgttagtccc aaaaataaaa gataaggaat aaaaataacc gtgttactta agataaaggg
    76201 cgttgtttgt gtaaaattac gcccaaatcg tttgagtttt tcgaacatga caatggctcc
    76261 ttaaacagtc ttataactaa tctgctcttc gattttagca ttaaagggga aataaaccaa
    76321 ttttacagaa tatattcttg taaaaaaaaa tacagaaaga agcataagct tctttctgca
    76381 tttttacggt acttgattac tcaagcagac aagcccttct ccacaaaagt cttgcccgac
    76441 acccctgcat ccggcaggtg ataaagatag aaatcaatta tcctgtgcct ccaaaatcaa
    76501 ttaggagctc tgtaaaccag agtccttctc gacccgtcgc ataaatttac tttaacacag
    76561 ttcctatgtt tatgcgagag cttttttcat tttataatcg accatttgaa atccttgacc
    76621 gatatctgaa ataagttctt ctgtaatctc aaatccttca tgcttataaa cctcgattgc
    76681 ttgatgattc ccctgattca ctcgcaaaaa gatctctctt tcctttgttt gcgcagcaag
    76741 ttcttcatac cagcgaaaaa ctgaagaagt caatccttga ccacgtactt tatttagcaa
    76801 atataattta cttaaataaa tttttccatt tatctcttca taagcagtgt aaccgacagt
    76861 cttttcttcg ctttttaata aaaagtagga cacaccttct gtcagttctt tttgaatctg
    76921 tttttttgac tgataagttt tcagcatgta atccacttgt ttttgaccaa tgatcggcac
    76981 aaaaacttcc ggccatactt cttgtatgac tgccatcagc tcatccagtt cattttctgt
    77041 tacttttatt ttttccatac acccacgtcc ttttttacat tataaatgac cttgcgcttg
    77101 atggaataaa aagagcatga tcatgctata cacacccacc gaccacggtt tcaagaaaat
    77161 cacaatctgc ataaattccc taaaagataa tttcgtcaaa ccagctacta gacagatcaa
    77221 gtcgtctggc gcaaagggaa gcagcaaggt aacaactagt acttttttaa tatctttcgt
    77281 ttttcttgtc agcaattcat caaatttctg atactttttt ggcgataaaa taagctggac
    77341 aaaggctacg ccatagtaac ggacaagcaa aaatccgatt gcttccccaa tcactaggcc
    77401 aatacaacta tataaaagcc caaatccatt gccaaacatc agcatgcccg ctactgagga
    77461 gatccctccc ggcaaaatag gaatgatcac ttggatcact tgcaacagaa caaatatcat
    77521 cactgcatac ttgccaaatt gctggataaa cgcttgcaaa gacgtgctcg atcgaaaaat
    77581 cccatgatga taagcataaa gaatcaacac aaagaaaaaa aacagaccaa taatgggcat
    77641 gatctgaaga catttttgta aaactgatcc ttttttagac atctatattc cccacatctt
    77701 ttttcattta ttaaaactgc ctcctt
//
LOCUS       DACDIH010000005        66264 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDIH010000005 DACDIH010000000
VERSION     DACDIH010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 66264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.005"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1240
                     /locus_tag="IV281_000334"
     CDS             <1..1240
                     /locus_tag="IV281_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP8779377.1"
                     /translation="VSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGG
                     LGIMMISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGT
                     FFSIYFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFL
                     IFVGGIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEK
                     DHLFQDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVG
                     GGIRTTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFL
                     SATEEQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLF
                     VPKETRDLGYEYPSEKIIIG"
     gene            1463..2815
                     /locus_tag="IV281_000335"
     CDS             1463..2815
                     /locus_tag="IV281_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP8779378.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            2808..3506
                     /locus_tag="IV281_000336"
     CDS             2808..3506
                     /locus_tag="IV281_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP8779379.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(3568..4719)
                     /locus_tag="IV281_000337"
     CDS             complement(3568..4719)
                     /locus_tag="IV281_000337"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP8779380.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(4732..5169)
                     /locus_tag="IV281_000338"
     CDS             complement(4732..5169)
                     /locus_tag="IV281_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8779381.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(5184..6950)
                     /locus_tag="IV281_000339"
     CDS             complement(5184..6950)
                     /locus_tag="IV281_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP8779382.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNGMVNPQKYESIAQYLKQTA"
     gene            7214..7891
                     /locus_tag="IV281_000340"
     CDS             7214..7891
                     /locus_tag="IV281_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8779383.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            7894..9021
                     /locus_tag="IV281_000341"
     CDS             7894..9021
                     /locus_tag="IV281_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8779384.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(9221..9661)
                     /locus_tag="IV281_000342"
     CDS             complement(9221..9661)
                     /locus_tag="IV281_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8779385.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(9672..11705)
                     /locus_tag="IV281_000343"
     CDS             complement(9672..11705)
                     /locus_tag="IV281_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8779386.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(11707..13410)
                     /locus_tag="IV281_000344"
     CDS             complement(11707..13410)
                     /locus_tag="IV281_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8779387.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(13504..14454)
                     /gene="manA"
                     /locus_tag="IV281_000345"
     CDS             complement(13504..14454)
                     /gene="manA"
                     /locus_tag="IV281_000345"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP8779388.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(14493..15518)
                     /locus_tag="IV281_000346"
     CDS             complement(14493..15518)
                     /locus_tag="IV281_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8779389.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(15727..16596)
                     /locus_tag="IV281_000347"
     CDS             complement(15727..16596)
                     /locus_tag="IV281_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP8779390.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(17300..19192)
                     /locus_tag="IV281_000348"
     CDS             complement(17300..19192)
                     /locus_tag="IV281_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8779391.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(19189..20916)
                     /locus_tag="IV281_000349"
     CDS             complement(19189..20916)
                     /locus_tag="IV281_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8779392.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(20932..21582)
                     /locus_tag="IV281_000350"
     CDS             complement(20932..21582)
                     /locus_tag="IV281_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8779393.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            21786..22082
                     /locus_tag="IV281_000351"
     CDS             21786..22082
                     /locus_tag="IV281_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8779394.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            22274..22894
                     /locus_tag="IV281_000352"
     CDS             22274..22894
                     /locus_tag="IV281_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP8779395.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(22986..24152)
                     /locus_tag="IV281_000353"
     CDS             complement(22986..24152)
                     /locus_tag="IV281_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8779396.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(24164..24517)
                     /locus_tag="IV281_000354"
     CDS             complement(24164..24517)
                     /locus_tag="IV281_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8779397.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            24645..25667
                     /locus_tag="IV281_000355"
     CDS             24645..25667
                     /locus_tag="IV281_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP8779398.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(26106..26540)
                     /locus_tag="IV281_000356"
     CDS             complement(26106..26540)
                     /locus_tag="IV281_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779399.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(26650..28509)
                     /locus_tag="IV281_000357"
     CDS             complement(26650..28509)
                     /locus_tag="IV281_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8779400.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(28627..29787)
                     /locus_tag="IV281_000358"
     CDS             complement(28627..29787)
                     /locus_tag="IV281_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP8779401.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(30094..31518)
                     /gene="araA"
                     /locus_tag="IV281_000359"
     CDS             complement(30094..31518)
                     /gene="araA"
                     /locus_tag="IV281_000359"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP8779402.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(31534..32232)
                     /locus_tag="IV281_000360"
     CDS             complement(31534..32232)
                     /locus_tag="IV281_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8779403.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(32189..33850)
                     /locus_tag="IV281_000361"
     CDS             complement(32189..33850)
                     /locus_tag="IV281_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP8779404.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            34068..35468
                     /locus_tag="IV281_000362"
     CDS             34068..35468
                     /locus_tag="IV281_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP8779405.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(35720..36799)
                     /locus_tag="IV281_000363"
     CDS             complement(35720..36799)
                     /locus_tag="IV281_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8779406.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(36885..37844)
                     /locus_tag="IV281_000364"
     CDS             complement(36885..37844)
                     /locus_tag="IV281_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP8779407.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(37856..39379)
                     /locus_tag="IV281_000365"
     CDS             complement(37856..39379)
                     /locus_tag="IV281_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8779408.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(39389..39952)
                     /locus_tag="IV281_000366"
     CDS             complement(39389..39952)
                     /locus_tag="IV281_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP8779409.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(39958..40821)
                     /locus_tag="IV281_000367"
     CDS             complement(39958..40821)
                     /locus_tag="IV281_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8779410.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(40835..41731)
                     /locus_tag="IV281_000368"
     CDS             complement(40835..41731)
                     /locus_tag="IV281_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8779411.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(41906..43177)
                     /locus_tag="IV281_000369"
     CDS             complement(41906..43177)
                     /locus_tag="IV281_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8779412.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(43167..44684)
                     /locus_tag="IV281_000370"
     CDS             complement(43167..44684)
                     /locus_tag="IV281_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8779413.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQET
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            44882..45772
                     /locus_tag="IV281_000371"
     CDS             44882..45772
                     /locus_tag="IV281_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP8779414.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(45845..46669)
                     /gene="nadE"
                     /locus_tag="IV281_000372"
     CDS             complement(45845..46669)
                     /gene="nadE"
                     /locus_tag="IV281_000372"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP8779415.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(46681..48153)
                     /locus_tag="IV281_000373"
     CDS             complement(46681..48153)
                     /locus_tag="IV281_000373"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP8779416.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(48790..49152)
                     /locus_tag="IV281_000374"
     CDS             complement(48790..49152)
                     /locus_tag="IV281_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8779417.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            49375..50127
                     /locus_tag="IV281_000375"
     CDS             49375..50127
                     /locus_tag="IV281_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8779418.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(50267..51190)
                     /locus_tag="IV281_000376"
     CDS             complement(50267..51190)
                     /locus_tag="IV281_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8779419.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(51205..51477)
                     /locus_tag="IV281_000377"
     CDS             complement(51205..51477)
                     /locus_tag="IV281_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779420.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(51821..53008)
                     /locus_tag="IV281_000378"
     CDS             complement(51821..53008)
                     /locus_tag="IV281_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP8779421.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            complement(53421..55046)
                     /gene="groL"
                     /locus_tag="IV281_000379"
     CDS             complement(53421..55046)
                     /gene="groL"
                     /locus_tag="IV281_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP8779422.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(55097..55381)
                     /locus_tag="IV281_000380"
     CDS             complement(55097..55381)
                     /locus_tag="IV281_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP8779423.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            55606..56268
                     /locus_tag="IV281_000381"
     CDS             55606..56268
                     /locus_tag="IV281_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8779424.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(56344..57636)
                     /locus_tag="IV281_000382"
     CDS             complement(56344..57636)
                     /locus_tag="IV281_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8779425.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            57806..58435
                     /locus_tag="IV281_000383"
     CDS             57806..58435
                     /locus_tag="IV281_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAP8779426.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(58538..59347)
                     /locus_tag="IV281_000384"
     CDS             complement(58538..59347)
                     /locus_tag="IV281_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8779427.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(59402..60271)
                     /locus_tag="IV281_000385"
     CDS             complement(59402..60271)
                     /locus_tag="IV281_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779428.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(60272..61588)
                     /locus_tag="IV281_000386"
     CDS             complement(60272..61588)
                     /locus_tag="IV281_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779429.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(61585..63420)
                     /gene="walK"
                     /locus_tag="IV281_000387"
     CDS             complement(61585..63420)
                     /gene="walK"
                     /locus_tag="IV281_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP8779430.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(63425..64129)
                     /locus_tag="IV281_000388"
     CDS             complement(63425..64129)
                     /locus_tag="IV281_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8779431.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(64319..65179)
                     /locus_tag="IV281_000389"
     CDS             complement(64319..65179)
                     /locus_tag="IV281_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8779432.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(65166..65735)
                     /locus_tag="IV281_000390"
     CDS             complement(65166..65735)
                     /locus_tag="IV281_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP8779433.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            66116..66188
                     /locus_tag="IV281_000391"
     tRNA            66116..66188
                     /locus_tag="IV281_000391"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:66149..66151,aa:Lys,seq:ctt)
BASE COUNT        19179 a        13738 c        11588 g        21759 t
ORIGIN      
        1 tgtttctttt gtggacatgc tgttcatggc aatcagtaca gtgagtgtga ctggactatc
       61 gacttttgat atcaattctg tgttcaatga taacggtatt atactgctgg aaatattatt
      121 tcaagttggt ggtttgggga tcatgatgat ttctactgct tttgtcattt tttctaaacg
      181 acggatcaca ctgaaacagc gacagctgat catgacggat atgaatcaac cgcgtttgtc
      241 tggtatcgtc cgactgattc ggatcacttt tgctatttta atctggtttc aattactttt
      301 tgggacattc ttttctattt atttttatta cagaggctat tttgatcgtt ggcgtgatgc
      361 cgttttttac ggtttttatc aggcgatatc ggctgtcacg aactctgggt ttgacgtcac
      421 tggggattcc atcaagccct ttgcacatga ctattttttc ttgatcctga tcatgttttt
      481 gatttttgtc ggaggaattg gttttcctgt attgatggag tgccgggaat ggctattata
      541 caaacgttcc aatgcgaagc ttccgtttcg tttttccttg tttaccaaat tagctgtgtt
      601 agcttttgtt atcttgtttg tttcgggaac tgtcttgatt tatctattgg aaaaggacca
      661 tttattccaa gattccaata tgagtgtcaa atggatcaat tcgatgtttt attcgattac
      721 gactcgtaat gctggtctgc agattcacga tttgggtgat tttcaaatca caacactgat
      781 cattttttct ttactgatgt ttatcggatg cagtcctagt tctgtaggcg gcgggattcg
      841 aacaactact gtagcaatca tcggtttgta tctatattcc ttcctcaaaa gtgaagataa
      901 catcaatatt tttggacgga gaatcgatca agatgatgtg cgaaaatcag tcgttgtctt
      961 tatgctttct cttggtatgt gtttcttttg tatcgttttt ttatctgcta cagaagaaca
     1021 aacgctgatc tcgatcatca tcgaagtaac ttctgcgttt ggaacaactg gtttatcttt
     1081 agggattacc ggagacttgt ctgttgtggg caaaatcacg attgctacac tgatgtttat
     1141 tgggcggatc ggaatgctat atacattgat gttattcgtg ccaaaagaaa cacgagatct
     1201 aggctatgaa tatccaagcg agaaaattat tatcggttaa aatgggaata taggtaagag
     1261 ggaataataa aatcacagca gcttgaaaca atcattttgt ttcaagctgt ttttttgtta
     1321 aatcatgcga ttttgtccga tttcatgcac tttcagccgt aaccttttct taagaataag
     1381 aaaagcaatt taaccttttt ttcaacatat ggtatgatta gttgaattta ttcgtcagca
     1441 aatcgttttt ggaggttaca taatgggaat acgcagtcat tttgcgattg cagttggaaa
     1501 aacgtcgcaa tgggcattaa aaactttttt taaaggcgga tcaagtttac caggtaaact
     1561 agcgctgaaa gtcgatccac atatactaga tagtttggca aaagactacc aagtcgtcgt
     1621 tgtcaccgga acaaacggta aaacattgac taccgcttta acagtcaata ttttgcgcca
     1681 gcaattcgat gaagttctaa ccaaccctac tggtgcgaat atggtacagg gaattgtatc
     1741 gactttcttg caggcaaaac caaaaaaagg acagaaaaag tttgctgtcc tagaaataga
     1801 tgaagcaagt ttgagcaaag tcacagaata tatgaaacca gaactgttct tatttacaaa
     1861 tattttccgt gatcaaatgg atcggtatgg agaaatctat acgacctata aaatgattgt
     1921 agacggtgcc gcaaagtcac caaatgcaac gattatcagt aacggtgatt caccaatctt
     1981 caattctatc gaaacagtca atcctagaaa atattatggc tttgaccatg aagaagatcg
     2041 tgaacaaatg gctcattaca atacagacgg tgtcttatgt ccaaattgtc atcacatctt
     2101 gcattataaa atgatcactt atgcaaactt agggaaatat tactgtccaa attgtgactt
     2161 caaacgtcct gaattggatt atcgattaac tgatatggta cggatggaca atgtgtcagc
     2221 agatttcgtg atcgatggtc atgaatacgg aatcgaagtt ggcggtatgt ataatgtcta
     2281 caatgcttta gcagctactg cagtggcaga gtacttcaat gtcgatcctg ataaaatcaa
     2341 acaaggttta ggatatgacg agaaagtatt cggccgacaa gaagtcatca atatcaatgg
     2401 gaaaaaatgt acattagtac ttgtcaaaaa tcctgtaggt atcaatcaag taatcgatat
     2461 gattggtcta gctccttatc cattttcttt agtggcttta ttaaatgcca attacgcaga
     2521 cggaatcgat gtcagctgga tttgggatgg taattttgaa aactttgccg atatggatat
     2581 cccagctgtt atttctggag gagaccgtca tacagatatg actttacgtc taaaagttgc
     2641 cgggattcca gaaaataaat tgacagaaac aaaagaatta ccagaagtaa tcgaaaaaat
     2701 caaagaattg ccaactgagc atgtctatat tttagctact tacactgctg tattgcagct
     2761 tcgtaaagaa ctagctgctc aaggatttat caaaggagga atgaaccgtg gctaattatg
     2821 aactcaatat cgcccacctt tatggaaatt tgatgaacac atatggtgat aacggaaacc
     2881 ttttgatgtt gaaatatata gcagaaaaaa tgggggtttc ttgccatacg gagatcgtta
     2941 gtatacatga accttttgat gctgataaat atgacttggt tttctttggc ggtggtcaag
     3001 actttgagca attaatcatt tcaaaagaca tccagaaaaa gaaagattct ttgatcaact
     3061 atatcgaaaa tgatggcgtc atgttagcca tttgtggtgg ctaccaattg ctaggtcact
     3121 actacatggg agctaatgga gaaaagatcc aaggaatcag tgcactggat cactacacgt
     3181 taagtcagga aaataaccgg tttatcggtg atatcgttat ccataacgaa gaattcaatg
     3241 aaacttatta tggatttgaa aaccacaatg gtcggacttt cttaggtgaa ggtgaacgac
     3301 ctttggggaa agtggtacaa ggtcaaggaa ataatggtga agaccaatct gaaggtgtca
     3361 tttaccgaaa tgtcttcggt tcttatttcc atggacctat ccttgcaaga aatgagaacc
     3421 tggcagctcg tttgatccgc ctagctttag aacaacgtta cggtaaagaa ttggatctgc
     3481 cctatcttga aggacaaaca gcctagttat tcgaaaaagg ggaatgtgac aaaaatttcg
     3541 tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac ggttcacgtt
     3601 ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt gaatcaattg
     3661 ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat cttcaggatc
     3721 atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca agttatgttc
     3781 ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac gtaatggtgt
     3841 ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat gaatgatttt
     3901 ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca gcaaacttcc
     3961 tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca ttgcttcatc
     4021 aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac cagtattgac
     4081 actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa ccccttttaa
     4141 ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga aaggttccac
     4201 ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga ctgctgcatt
     4261 tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat acacttcttt
     4321 ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta aagatgttgt
     4381 gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa taaaaggtaa
     4441 gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt ggattacttt
     4501 ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt tttcttttga
     4561 ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa tgatcgtgtg
     4621 attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc caataaatcc
     4681 tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc cttaagcaat
     4741 tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga caacattgtc
     4801 catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat ctccgactcc
     4861 tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa aatctacacc
     4921 atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg cttcatctgt
     4981 tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt ctctatctac
     5041 catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct gaccgcaata
     5101 gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt cgaatggcaa
     5161 actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc aatcgattcg
     5221 tatttttgcg gattgaccat gccgttgact gataaatgaa tggattgagg tgcagccatc
     5281 tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc atcttttaaa
     5341 tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa tatatctttg
     5401 agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag tagaatctct
     5461 ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc aatttcagaa
     5521 actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg aggtgaattc
     5581 aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc tctttcttgt
     5641 agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct gattgcttga
     5701 ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat cgaagcgccc
     5761 atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac gccagagaat
     5821 gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc ctttgtctct
     5881 tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa aattactgca
     5941 gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg aaggtaagaa
     6001 ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg cgctcttagt
     6061 ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat aactgataag
     6121 aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc gcctaaaaag
     6181 tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa caaagtaaaa
     6241 gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag aatagatttt
     6301 ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt gattgcatta
     6361 ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat caaatgggca
     6421 gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac aactggacca
     6481 atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga aaaattattg
     6541 actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa tttcttgatg
     6601 gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat atctgttccg
     6661 acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc agcaattact
     6721 tttccgccag tataaccgat caataaagga atcagataag ttagcattgg gcttaccatg
     6781 gtagcgaaag cctcatttgg taaataacca tctgggataa atagagccgt taatacaccc
     6841 caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact gcctaatttt
     6901 tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat tttgtctctc
     6961 cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat agtcgtactt
     7021 tggcacgaaa ctttgtgcca aaggtttgag tatgtcaaca aaataaaaag atttcggtat
     7081 cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata tgcaaacaat
     7141 aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta aattttatat
     7201 tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg gtaaaaaacg
     7261 tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt tgattgcccc
     7321 gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc caacaactta
     7381 tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc gccggtcttt
     7441 cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg attatttgac
     7501 tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc aacgattgaa
     7561 gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga aacaacatgt
     7621 cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg aaccgttgaa
     7681 cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat ggcagaaaga
     7741 ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga tttgttctaa
     7801 agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg aagagatttt
     7861 agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt tgaacgcaat
     7921 ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt gctctttgca
     7981 ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact gaatcataac
     8041 tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga gaaagaattt
     8101 gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc aaaaaacaca
     8161 gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat actggatcga
     8221 ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg tgtcgagttg
     8281 ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt gccagcgcat
     8341 ctctctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag ttttttgatt
     8401 ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag aaagcaggta
     8461 atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat ccgttttacc
     8521 atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt ctctatacta
     8581 gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat cattggtcag
     8641 gagatacgaa tcctgccgat gtatgagtat cttttccaga gctttctgtt cctgttgttg
     8701 tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga acattctcta
     8761 gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt gttttcttcg
     8821 attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga ttttcaaaat
     8881 gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac tttcatgaat
     8941 ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat gggcactatc
     9001 tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa aaccttgtca
     9061 ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa gcttgcatcc
     9121 gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg gttgaagtta
     9181 ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt ccatgttcaa
     9241 aaatcatttg gacttcttca atggtttccg catcactgag cgccattact tggtcgatgt
     9301 ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt tttaaagcca
     9361 ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc acaccatcag
     9421 gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca gtcccatgtg
     9481 gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg atcattgatt
     9541 ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc tcggtcaacg
     9601 cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt agctgtatca
     9661 tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa ctgataaacg
     9721 atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat gattgctcca
     9781 ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc cagcataact
     9841 aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa cggatgtttc
     9901 aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg gaataacccc
     9961 atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag ccggcgatga
    10021 accacctctt catccgttac tagtccttca ggaaaataag gaaataagct gcgaatcgtt
    10081 tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag taattgattg
    10141 atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca ctcgttattg
    10201 ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc ttcttttagc
    10261 tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc accgtatcct
    10321 ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat tttttttaaa
    10381 tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt caatcgtagt
    10441 tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact gatattgctt
    10501 tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc gatctcttct
    10561 tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc attcgcaact
    10621 tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc ttcttgtaaa
    10681 acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa agtttcatct
    10741 tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt tactaaataa
    10801 gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt cgtgaagtca
    10861 tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt tgtctcagaa
    10921 gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc cagactatta
    10981 tatgtctcat ttgtcagtaa atgatttttc gtcacacgct ccatcgtcaa tgtcagcatc
    11041 aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat caattcttcc
    11101 ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa aaaataattc
    11161 agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc taattctaat
    11221 acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta tccctggttc
    11281 cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat atctgcataa
    11341 aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa cggctctgtc
    11401 gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa gtcattactt
    11461 ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc aatgacaaat
    11521 gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac ttcaagatta
    11581 tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat tttttggata
    11641 gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct gggcgataaa
    11701 tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta cccaatgttc
    11761 aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta gttctctttg
    11821 tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt ttgagataac
    11881 aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca tagtttgtga
    11941 gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgacgttt tttgttctgt
    12001 gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt cttttattcg
    12061 tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac tttgaggtac
    12121 tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat ttacttggtt
    12181 attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg ctgctaattt
    12241 ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc aagcaaaaat
    12301 gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg ttttgttctc
    12361 tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca caaatgtact
    12421 gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg caaaaatcac
    12481 gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt gaaatatcaa
    12541 cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca gcaagacaaa
    12601 tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc cactcgcatt
    12661 tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta aaccaggtcc
    12721 aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga attcgattcc
    12781 taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt ttagaggttc
    12841 taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca caacggaggc
    12901 gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa ttatacaaat
    12961 aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc cagccttgaa
    13021 tttcacatgg aatctagaaa caaattgttc atatacgatg ccggcaagcg gcccgataat
    13081 catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag aagcaattgc
    13141 accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac tgatcaagat
    13201 gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt gcaaatcgcc
    13261 agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac ttagatttgg
    13321 catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag acacgcgact
    13381 gctgttgttt gctttccatt tgtattccat ctttttcacc agacttctat ttattgatgg
    13441 acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc agctatctgg
    13501 gaactatttg cctggttcag acgcaatgat tgctagttca ccttggaccg cccactcaga
    13561 aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt ttccgtcaac
    13621 gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt gttttttgaa
    13681 agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt ctaaataagt
    13741 gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg ttttcgcagg
    13801 gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc cttgcgcatc
    13861 ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg tttctaaaat
    13921 catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga aatcaccttt
    13981 tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca tctcagctag
    14041 ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt cagcatcgat
    14101 gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat aggcatcatc
    14161 tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca ataatggaaa
    14221 cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt catctaatct
    14281 tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg cactgatcgc
    14341 ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac gtaaacgact
    14401 tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct gcataattca
    14461 aactcctctc atttttcata gctattatag cattatttcg gtaataccgc tttctctttc
    14521 aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg atcgacagga
    14581 tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat caagaaaaac
    14641 gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc cttttttaag
    14701 tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag taaagaatca
    14761 atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa atacatttgg
    14821 acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc atgttcagcc
    14881 aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga aggcagttgt
    14941 tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat cattcctaat
    15001 ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat ttcctctagc
    15061 atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc tagttgctca
    15121 cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt atctctgttt
    15181 ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt gattgtctga
    15241 ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt tccatcttct
    15301 aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc tgcatcaaac
    15361 aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc ggctgtaaat
    15421 gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa tccttcttcc
    15481 atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg cttttctatc
    15541 agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt tttacacaaa
    15601 aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt tctatctcta
    15661 gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc gttaagaaag
    15721 aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct cactgacgaa
    15781 ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt tgtctaagaa
    15841 gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt cagtatcgat
    15901 catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa ttgcgcctcc
    15961 gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga tgccctcttc
    16021 ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt ttgttaagtt
    16081 agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac tgaatttttc
    16141 ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc tttggtcgtt
    16201 cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga gcaaaatccc
    16261 catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac ggatacgaca
    16321 aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt tcagcttttt
    16381 gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca tttcacttcc
    16441 agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat caaagccaac
    16501 aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg ctacactttt
    16561 taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc tctacgtcaa
    16621 gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt tgttaaaaaa
    16681 aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa aactcttata
    16741 aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa acaaaaatag
    16801 ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16861 ttaaggaaga ggttgtgaaa aagtcgctta gtttcgagta gtaagaaaaa ttttgtgaaa
    16921 atggctttct acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
    16981 cgtttaccaa gaggttgtga gcctgccgtt taactccgag tcacaaggag caatttcaaa
    17041 aaacagcttc tcaggtttgt tgactattgt gacttgtgac cgaggagttg gcaggagaac
    17101 accgtttaag gaagaggttg taaaatcagc ggtttgacct gagtattaga gatacaaaac
    17161 ggaaaaatag cttctcatat tttgccgttt tttgagctaa taccgaagga cagctgattg
    17221 aacaccgttt attcggaaaa agggactgtg acacgacttt tgtcacagcc cctaaaaaaa
    17281 ataataaatt tgtactattc tatgaagctt cttcttcaga aaactgactg ttataaagat
    17341 ctgcatagaa tccgtctttt tccatcaatg tatcgtggtt tcctgtttct acgatcgaac
    17401 catgattcat cacaataatg ttgtctgcat cgcgtattgt agacaagcgg tgagcaacaa
    17461 cgaaacttgt ccggttttcc aataggcggt tcatcgcttt ttgaatcagt atttctgttc
    17521 gtgtatctac actagatgtt gcttcatcaa ggatcaggac atctggattt gctaagaatg
    17581 cccgcgcaat tgtgattagc tgacgctgcc cttgcgaaat attgcttgca tcctcgttta
    17641 atacagtgtt atatccttct ggcaatttac gaacaaaatc gtctacgtga gcagctttag
    17701 cagcctcaat gatttgttcg tctgttgcat cgtcgttccc atatttgata ttgtcataga
    17761 tacttcctgt aaacagccaa gtatcttgca gtaccattga aaactgtgcg cgcaattcat
    17821 cacgtgaaag atcacgggta tctacaccat cgtaacgaat actacctcca gaaacatcgt
    17881 agaaccgttc aagaaggttg atcagtgtcg tcttccctgc accagtcgga cctacaatag
    17941 cgaccatctc accaggtttg acatccaagt taaaatcagt cattagcaag ttatctggtg
    18001 tgtagccgaa ttgaacattt tcaaaacgaa ctttgtatgg tgtatctttt tcttctggaa
    18061 ggccagattt ttcattggac atctcttctt catccaacac ttcaaatacc cgttcggctg
    18121 aagcaactgt tgattggata gtgttcatca aacttgcaag ttgagtgatt ggttgagaga
    18181 actgtgttgt atattgcaag aacgcttgaa catcccctaa tgtcatgttc ccatttgcaa
    18241 cttttactcc gcctagtaca gcgacgaaca catagcctag atttttaacg aaattcatca
    18301 acggcatgat gattgccgaa ataaattgag ctttccaacc agcggcatac agattctgat
    18361 tttctttttc aaatacttcc tggtcttttt cagtatgatt gaatgtttta acaatggtat
    18421 gaccaccata tgtttcttct acttggttat tcaataaacc taaacttttt tgctgatctg
    18481 cgaaaaacac ttgtgatttc ggcgctacga tcatcacgat gatcaagctt aaagggatca
    18541 tcaataacgc gactaacgtc aattgccagc tgattgaaag catcatcacg atgaccccaa
    18601 tcaatgtgac tgcactagtt acaaattgag tcaaattttg ctgcagcgtg cttgcgatgt
    18661 tatccatatc attgattgct cgagacatga tatccccatt tgtatgtgta tcataatagg
    18721 aaatcggtac acgattcatt ttggcttcca aatcatgacg taattcataa actgttcgtt
    18781 gggaaacacg tgtcataata aattgctgta aaaagttgaa tacagcagaa atcaaataca
    18841 tcacgattac tgtcgcaatg atctgcgcga ttttatcaaa gtctatcgga aattttgtta
    18901 cttgttggcc ttgttgcatc tgttgagcgc cagccattac acctttgaat atttctgtgg
    18961 ttgctttccc caagaccttt ggtgtttgga tttggaagat cacggctgct attgctagta
    19021 caaatacagc gatgatcgca accatacgtt tagacatata gccaaacaat cggcgtaccg
    19081 ttccccagaa atttttaggc ttttcacctt ttacaccgag atttctgcca ggtccaccat
    19141 gtcctggacc gcctgctggt ccatgagagg gacgtgtgtt tgttttactc atgctaagtc
    19201 ctcctctctt agttgtgaat gaacgatttc ttgataagtt tcgtttgttg ctaatagttc
    19261 ttcatgtgtt cctttgccaa ccagttttcc ttcatctagc acaaggatca tatctgcatc
    19321 cattactgta cttactcgtt gagcaactaa aacggtgata ctttctttca tattcgtttt
    19381 caatgcttgg cgaagattgg catccgtttt gaaatccaac gctgagaagg aatcatcaaa
    19441 cacatagata tctgctgttt tgactaatgc ccgtgcaatt gccagacgct gtctttgtcc
    19501 accagagaag tttcctcctc cttgttccac atggctatct aagccatctt ccatctctga
    19561 aacaaaatct ttagcttgtg cgatttctaa tgctttccaa atcatttcat ctgttgcatc
    19621 cggcttacca tactgcatat tgtctcggat cgttccagag aacagaactg ctttttgagg
    19681 aacaaagccc attaattctc gcaaattata ttgagtcatg tcactaatgt ccgttccatt
    19741 tatttggatc gaaccagatt cgatgtcata taatcgaggg atcaaattga ctaaggtcgt
    19801 tttacctgaa ccagtccccc ctatgattgc gacgatctcg cccgacttgg cttggaaatc
    19861 aatatcttcc aatgcaagtt tttctgctcc atgatatcga taattgacat gattgaaggc
    19921 caatgttgcg ttttttccag caaaagaaac tgttttagga ttttcagggt catggatacc
    19981 gattttttca tccaacactt cattaatacg gtcagctgaa gcttgtgcac gaggcaccat
    20041 gataaaaata gcggaaagca tcataaagct gatcaagatc tgcatcgcat aagtcataaa
    20101 tgcaatcaga ttaccgactt ccaaagtcat atctgctatg tagtgtccac caaaccaagt
    20161 gattgctacg tttgtaccgc tcatgatcaa tgtcatcatt ggcatcatca atgcgacgat
    20221 cgtattaact ttgattgccg tatttgtata atctttattc gctaaatcaa aacgattttc
    20281 ttcaaagcgt gtcttatcga atgctcgaat cactctgacc cctgtcaatc cttctcgaaa
    20341 taccaggttt aaacggtcag ttttcttttg catacttttg aataatggaa cagcaaagta
    20401 catgattccg ccgatcaaaa taatcattac tgggatgaca actaagaaga tttttgtcaa
    20461 ctgatgatct ttttgataag caagtatgct cgcgccaatc aacgtgattg gtgcattgat
    20521 catcatacgt aaaaacattt gtgtaaccat ttggatttga gtcacatcat tggtcgttct
    20581 tgtgatcaaa gaggccgttc catatttatc aaacgcatct tttgtcagac tctctgattt
    20641 tttatagaca tccgtacgca attgcttacc cagtttctgt gattcacgag tagcaaaaaa
    20701 tgtatttgcg atagcagcta agatactgat caaagaaaag ccgatcatca caaaacctgt
    20761 atgccaaata taatcgacat ctccttgcgc taccccttta tcaataatat tggaggtaag
    20821 tgtcggcaaa tataaatctg ccagtacttg gatcaccatg aaaactgcag ctgcaattgc
    20881 tgaagtcaag gagatccgtt ttacgatttt tagcatctag ttcccttctt tctatttata
    20941 tactccgttt ttcagccaag aaagtttcaa attgaattca ctgattgctt gctctactga
    21001 gtaatcttca gatgttgaaa ataagcgata cgaatgcgca attgttgtaa atacagtatt
    21061 catcactaat tggatcagta attccaattc atgatcattt tttactttca gcttcgtccg
    21121 atcgatcaat tcaagcaact cttttgcatc ttgtttgtgt tctttatgtg gatcgtgagg
    21181 cgttttttgg cgactcgtat gaggagcgac ttttcttgag gcacggtaat ccatgttcat
    21241 aaacaagtta cgataaaaag aagcattctc gccttccaaa atctcataaa tcattttaga
    21301 aaaataggat tcgaatcctt tgaatagatc accatcggat tctttcatta gttgtaacat
    21361 atctttagag ctgtcacgtc tcattgtctc aaagtaataa aaataaagat cctctttatc
    21421 ttcaaaatac tgatagaaac tgcctctagg gatatccgct aatttgacga tattagcaat
    21481 cgaagcttct tttaagggta ctctagaaaa ctcgattctc gcagcctcga ttagtctttt
    21541 ttgtttttct ttaggcaaat ggaaaaatgt ctgtgtcggc atatggattc tcctttcttt
    21601 caagatgaca cattgtcata tgacgctatg tcattataac acctcctacc tgttttgcaa
    21661 gacatttgca tagttttttt cattatcatc cgatacatta aaaaagtgat aagaggaatc
    21721 cttccatttg aattaatgag agttgcccat taaaatagcc acatactaaa caaaggatga
    21781 gataaatggc ccaatcaatt agtgtccaag acttcaataa attgcctctc gatgctgaca
    21841 gcatcgtttt agatacaaga aataccttag attacacaaa aaaccactta agccactcgc
    21901 tttctatccc cgctgctatc ttgccaagac aattaaaaaa tctagatcca aagaaacttt
    21961 attatattct aagctataca ggcaggcgat cagaagcatt agccgatcaa cttactgcta
    22021 atggttttcg tgctgtttat atcattggtg ggatggaagc tcttcagtcg agtgttgctt
    22081 gaatattgct tgttagattc aaaagaggct gtgacaaaaa atcgtgtcac agcctctttt
    22141 ataccctgtc tttttttagt tatttattct tcaaccttat caatatgact tgatagcctt
    22201 ttctttttcg agtaaactga ttttgttagt catagttata acaatcatag caataaaagg
    22261 agaaggtaag catatggcat ctgttttagt agtcaaaggt catcccctaa ctgcagaaga
    22321 atcgcgcaca gttaaggcat tgacgtcttt tttaacaagt tataaagaaa atcatccaga
    22381 tgatgaagtg actgttttgg aactttatcg cgatgatatt ccggaaatcg atgaagaatt
    22441 attatcaggt tgggaagcat tacgtgctgg tgcagaattc acttccttat cagaaagtca
    22501 acaacaaaaa attgctcgtt tcaatgaatt gactgatcag ttccttgctg ctgataaaat
    22561 cgttatcgca aacgccttat ggaatctaaa tatcccaaca aaattaaaag cttggttcga
    22621 taccgtcaat gtagctggaa aaacgttccg ctatacagaa aacgggccag aaggattagt
    22681 gacaggtaaa aaagcactgc acatccaatc taatggcggt gtatacaacg gacaagactt
    22741 tgcctctcaa tatgtaaaag gtattttaaa ctttgtaggc atcgatcaag tagatcagct
    22801 attcatcgaa ggaatcgatt atgaccctga ccgtgcagat gagttgatgc aaaatgcatt
    22861 agataaagct gctgctttag gtaaatcatt ttaaaaattg agataaatgc atacaaaaga
    22921 gacagaggaa aaaatcttca aagcagcatt tgtgccttgg atattttccc atctgtctct
    22981 ttttgttatt ctttctgctt tttcaacgga ttttcttcat aaagagaaga gattgttgtg
    23041 taccattcaa aaacatgagt aatcactact ttagctgctg cataaacagg aatccccaag
    23101 atcacaccga tgattccgaa caatttccca gaagtcagca gaactaataa aatcgttact
    23161 ggatggattg ctaactgatt ccctagtact aatggagaga tcaagcgtcc ttctattgtc
    23221 tgctctactg caaaaacgat cagtactttg atgatcatga atggacctgc tacaatggct
    23281 aaaaagatag ccggaatcat cgctaaaaat gatcctaagt aagggatcag gtttaaaaaa
    23341 cccgcaataa tccccaacgt gacagcatac tctaaaccaa tgaccgcaaa accaatcata
    23401 aacatgattg ccacagcaaa agccacagtt agttgtcctc ttatataaga agaaacttga
    23461 ctattcattt cactcagcac attcaacgtc ggtttttgca tgcgtgtcgg taaaaatttg
    23521 acaaaataag gtgctaattg tttaccgtct ttcaatagat aaaataagat aaacggcatc
    23581 gtcagcagag ccactaaaat agtcgctacc gcgccgacaa aacttccaag actctgaact
    23641 gtactcttag atatatcttg gatcatatcg cctgccgaat tcatgaactt atcacctgct
    23701 gtttccaact gttcgcgaaa ttggttgaac agcgggtccc gcaaaatctc ggttaattta
    23761 ttatcaatcg tatctacata ttgagggaag ttactgatga agctcactgt ttgttcctga
    23821 atcttaggga tgatcactac gctcccccat gcgattagtg caacaaccaa aacaaacagc
    23881 ccaatgatac tgtaaagcct tggtactttt cgcttttcaa agtaatcaac taccggattc
    23941 atcaagtaat acaagattcc tgctaaaatg ataggcagac ctacgattgc taaaaattgc
    24001 cagattggtg caaacaaata tgaaactttt gtaaataaaa agacgatcag caatattaac
    24061 aatacaacaa gtaaggctgt gactacttga ttatttaaaa accagcgcca aaaccacgat
    24121 accggtctgt tcttcttatt ttttccttcc ataattcctc ctgttagtta taaaaaattt
    24181 ggcttcccaa accaatagca ggcagtctat gcggatgaag atagattcca tttgcaattt
    24241 gtccttcttc tggacaagca tcaaaaatca atgatacgtg agctactgtc tttaaattcc
    24301 cattggccat cggaccgaca taatcgatcg tatatacctg atcatcaaaa gcaattgttc
    24361 cgcctgcttt caaagaaaaa ggttcgtctt ttgatacttc ttgaatcacc gaatattttc
    24421 gtaaggcagc tgttgctgtt ttatcgaaca aaatcagcat gggttcttcc ttgtttattg
    24481 cttctgtccc aatttctgta atctttgcct gaatcattgc actctctcct ttaattgctt
    24541 tcaaaaacag tataacacat tctcattctt tagccgtgtg gaataattat gtgatagaat
    24601 aaaaacgaaa actatttcat tacaattagg aggaatcagc tcatatgatc gaaaagataa
    24661 ttttaggaac ctatacgcgt cgcgaaagta aaggaatcta tacgatcgac cttgatacag
    24721 aaaaagaaga attagccaac ctttcattag ctgttgaaga aaatagccct acctatgtag
    24781 caaaaagtaa agctggcaat ctttacacag ttactagtgt ggatggatta ggtggtgccg
    24841 gtgcgtacga taaggatttt cattttttga atgcggtaac agagtctggc gctcctttgt
    24901 gttatgtagc ggttgatgaa gcacgtcaat tactatacgg ggcgaactat cataaaggtg
    24961 aagtaaatgt ctaccatata ttaggagatg gcgaactgaa agcagcagat tcaatattcc
    25021 atcaagaagc gacaggtcct cataaaaacc aagatcatgc gcatgtccac tatacggatc
    25081 tgactcctga ccaacgatta gtcgtatgtg atcttggtac agatcgtgtg tatacttatg
    25141 atgtgtccga aaacggcaaa ttgaatcttg taactacctt cactgcagag ccaggaacag
    25201 gccctcgcca tcttgtcttt catccaaatg gacaattcgc ttatttattt ggtgaattag
    25261 acagcacagt ccgcgtgctt tcttataata aagaaacagg tgcttttgaa gaattacaga
    25321 aaatatcgac cttgccagaa gaattcgacg gtgaaaatgg aggggcagct atccgcattt
    25381 caaatgatgg caagttcctt tatgcttcta accgaggaca taactcgatt gctgtattcg
    25441 cagtttttga agatggttcc cacattcaaa acaagcaaat catctcaaca gaaggggatt
    25501 ttccccgcga tttcgcacta gatccaagca ataattttgt cgtgtgtgcc aatcagaaca
    25561 ccgataatct gacattatat cgccgtcatc ctgaaacagg attactggaa ttactgaaaa
    25621 aagatgtcta tgtaccagaa tgtgtctgtg tttattttga tcgttagtaa gaaaaggaga
    25681 atgaaatgaa tcagcaagac gtattagaca gactacggga agaactaaat attcctttct
    25741 ttgatgggaa aatcgaagat aaagagtata gcgaagagga atatcagaaa ctcaaagccg
    25801 atttacagaa ctactttgaa caatatgtac agaatgtaga aaactaatca gctttccata
    25861 ctatctgaaa agaacatcag gacaagactt ttccgtctat cctgatgttc ttttctacaa
    25921 ataaaaaacc gccgagatgg ttagactcga cggaaaagga gtatttactc gattttgagt
    25981 aagtacaagg ttgttgttgg ttataggtat gtcattatca taaaggattt tgatcactct
    26041 atcataaagg taatattaca tttatattag ataactctag tttttattgt tatcaaagat
    26101 ttcttttatt ttttcaacaa caaattagtc attttttgga tggcagctgt caattggtct
    26161 gtcgcttggt cttgttttgc ttcagatgta ttgaatagat cttcatctac ccctgattca
    26221 agatcgatac tttggatcat ttcttcacag cctgctacat atgaaagata agctttttca
    26281 aaatttttat gaatgcccat tgcttttgct ggtggacgca aagtactgac tttcataagc
    26341 attgcccgat atttttttgt tccttcttca aataacagga tgatctcttc aagagtcgat
    26401 ttgtccagtt cacttgtttt tccttcatca attgcgtttc tgatcacttc atatttcgga
    26461 tgcattcctt ctccgattgc ttctgtttcc ttgacgattt gatcgatgga ctgcacataa
    26521 tagccaatat tcggtaacat gttcgttttc ctactttctc cattgttgtt attatttatt
    26581 ctacttgttt ttatcctcaa atgacaagta cttgaaaaaa tttaatggtt tctatacagc
    26641 tcgccattct tattcgtgct caaagaaacc atctgctaaa ctatattgtt tccctgcgac
    26701 aatgtcatat tggatcaatt gatattcgtc gtaaatctct tgttgcgagc gattgaattg
    26761 cgcttcttta taccactgtc cgttttgata gtacagttct cgttcaaacg ccggaagttc
    26821 ctgttctaat gataaaagca gctgataaaa gccatttgtt gtctgattcg tctgttccat
    26881 tagattcgct gcaaaataaa acggactggt gattgcatcg tgagtcgttt gcttttctaa
    26941 ttctccttta ctgtcgaaca taagaaattg tgtttcatgc aatgtttgtt tgtcattttt
    27001 tgcttggatc gtatctgaat agatccctgg aagatgatcc ccccaaaaaa caactaaagt
    27061 ccttctagaa agattcttca attcttccgt gaactgcttc agtgccgtgc tgctatagga
    27121 aatatcttgc agataattct ctaagtcaag tgtcccactt ccatctgaag tttttgcagg
    27181 atagtctaga ttatcatact ttccatcgta gggcatatgt gtctgcatcg tgactaaatg
    27241 aatgaactga ggtgtttttt cttccttgag aagtgtcatt acttctttgt aagctgactc
    27301 atcagaaata tatggattat tctctatcgt atccgtatag gtcatcgtac gctcgctgat
    27361 gaactggtca aatcctaatg tctgataaac atcctctctt ttatacatcg aagtattgta
    27421 tggatggatc gctgtcgtct ggtatgattg tgcgtttaac gctgaaacga tagaaggaag
    27481 ctgatccatt tttggtacaa gcatcgtgta aggggtggtg agttgcgcat taaataacgc
    27541 catagaaaaa ccagtcaatg cttcaaattc gatatttgct gttccaccgc catagttttg
    27601 agaaagcata ttgccactat atgtttgatc agctacttca taataatctg ccaatggctc
    27661 accagatact tctacgccgt ttaatcgact aggatctgag aaactttcac tcatcacgaa
    27721 cacgatgttc ggcgactctt cctctacagt tttatttttt tcatcggcta gcttctggta
    27781 tttttcagtg atttctttga tcttttcttt cgaatatcct tcaggttcat ccattggttc
    27841 taccttcaga ttgtacaaaa acccaccgat aaaaccagtg ttatagtagt tcattttctg
    27901 gctgtaaggt atccatagtg ccgttttatt atatgctttt cgcaacaaat tatcgggatt
    27961 gttgaaatta ctgatatacc ctagcagtcc aattgccaca agcagtgtcg ttaatcggat
    28021 caattggaat cgccttcctt ttttcaggct tcgaaacatg taccagcaaa acaatcctat
    28081 cacggttcct gcggcagcta agatcactgt aaacagcata gtgccggtca tatctttcag
    28141 caatccgatt tcagtgatca tcttcaaatc gtctggataa atcggttctt gccgataact
    28201 cattttcata taatttgcaa aacctaatac accgatggct acgacatacg taagggaacc
    28261 taaagggaca gatcctgcta gtgcaatcag aaaaataaga ataatcagca agacgagaca
    28321 ggctaagaag aatttttccg tatgccaaga aaaagcaaat ttcaacgcaa ggtctacgga
    28381 tagatcattt tgacaccact gcaaaaataa attactaaaa acaacaagaa aaaagatact
    28441 gatgattgct gctgttcctt ttaaaatctt tttctgtttg gtcgaaagtc tgcttatata
    28501 tcttttcaaa tgcttgctcc ttcatttcta tcaataaaag aatgttgctt ctcttatttt
    28561 agttgtacga aattcgaaag taaagaacgg aaagaagaat taatgaaatc caaatattct
    28621 ctttctttat ggcctttctg gtttctttaa taagcggacc tttcgtactc cagaaatttt
    28681 ttctagctgt tgtttcactt gattgagctg ttcaaaatca gtactgtcta tgtcaatcaa
    28741 tatgtagaca tgctcattct tatggttacg cgatatatct gcgatcgcta gctcgttctc
    28801 attcagcaga gaaaatatct ctgctaatat attggttttc ttttgataaa atatagtgaa
    28861 tcgaaacggc gcttggaaaa acattcgtgc ggatggataa ttgaccgctt ctctaaccgt
    28921 accgaacaaa agataatctt ttaaggcttc aactgccaaa cgtccgctgt ctgccaaagc
    28981 ttcctgagtc gatcccccta gatgaggcaa cataagtata cggtcatttt ctaagaactc
    29041 ttcttctgga aaatcagtca agtatttagc cagatgttgt ttttccaatg cttgcataac
    29101 agcatattta tcaacaattt cacttcttcc tacattgata agtacagcat cttttttcat
    29161 tttttctata ttcgcctgat cgatcaatcc ttttgtatct tctgtcaacg gcagcatgac
    29221 tacgatgtaa tccgaggtag aaagaactgt ttctaaatct gcttgccgaa cataatccaa
    29281 ctgggcatcg cggatagaat aacccaagac atccatccct aagctatagc aacttagcgc
    29341 tacttttgtg cctatcgctc caagaccaag aagaccgatg gttttgcctt gcaattctcg
    29401 tcccacataa gcacttcttt tgttttctgc ctgttctaaa atattaggac ctgttagtgt
    29461 ttgtaccata cgacttgctt cgattatcgg acgggaagat aagagcaaac agcacaaaac
    29521 aagctctttt actgcattag catttacacc tggtgtattc atgacgatcg tcccgttttc
    29581 agaagctttt tcaacgttga tcgtgttcac cccaacacca gctcttgaaa ttgctagcaa
    29641 atctggtgta agccattcat ctttcacttt cgtactacga ataactattc cttcggctgg
    29701 caaatcatct attattttga aatattcccc ttgtaacttt tctatctctt gttctggaat
    29761 agcatcaatc aaatgaacgt gatacataaa accccctcct tactaacaag tatctcgaaa
    29821 tttctgcaat tagacaactg tttaccattt gacttcctag aagcaaaaag agcagttaga
    29881 gcagagtagg ctgagtgata aaaataaggc tatgacaagt tactgccata agccttattt
    29941 tcgagagcaa aatattgagg gtgagatatc ttgataaatg gtgttccgtt agctgacatt
    30001 gataataaga ctaaatttca aaaaagttga aaagcaaatt tcgtcaatct cttcttattt
    30061 cttaaaacta agtggctttt tcacaacctc tttttatcta ataaccactg tttccaactc
    30121 aactaatttt gcccacgttt caatttgatc tgttgttaga tttagcgaga caacggtatg
    30181 atgaccgcca cctgcctgaa tccatgaatg gacaccttca tggaaattcg gtttagtttt
    30241 ccataacaca cgtgcaacag gtaaattagg tgcttcagtc gttggttcaa aggcttctac
    30301 ttcattgatc agcagtttat aatgcgtacc gaaatctgcc atcgatacga ctaccccttc
    30361 tcctgctttc ccatcaaata caagacgcgc cggatcttct ctatctccca ttgataaagg
    30421 agaaacaacg attcttggtt tcgtcgcagc aagcgtcgga tctacttcca tcatatggga
    30481 ttccaagatt gcttcttggc cactagctag ctcgtaagta taatcttcca tgaatcctgt
    30541 atctttccct cgagccatga ttttcagcaa ccgatcaatc gctgcagttt tccagtcgcc
    30601 ttccccagca aaaccgtatc cttctgccat caaacgttgt acagctagtc caggtagttg
    30661 tttcattcca tacaggtctt caaaattagt cgtaaaagcg ttatagcctc cttcttctaa
    30721 gaaacggcga ataccgattt cctgctgagc ttgtactttt acatgttcct cccatgtctt
    30781 ttcttcataa tcaccatagt cgaaggtata caattccttg tactcttcaa ataattgatc
    30841 gacttcttcg tctgatacgc gatcgattac ttgtacaaga tctccgatac caaaataatc
    30901 aacagtccaa ccaaattgaa tctgtgcttc cactttgtct ccttctgtga cacccacgtt
    30961 tctcatgttg tcaccaaaac gagccacttt tattccaaaa ctttcattat aggctactgc
    31021 aacatccatc caatcagcaa tctctttttg gatctcaggt cgtttccagt agccaacgac
    31081 gattttattt tgtttattta atcgagcatt gatgaaccca tattcgcggt caccatgagc
    31141 ggattgattt aaattcatga aatccatatc gatcgttttc caaggaatgc tttcgttata
    31201 ttgcgtagct aaatgaagca atggtttttg tagaagcttt gtcccgcgga tccacatttt
    31261 tgctggtgag aaagtatgca tccaagtgat cacacctgct acttcttctc gataattgac
    31321 ctctttcatg atttttgtga tcgtatcagc agtaactgct aattcttgaa ggcggattgg
    31381 ataaggcaat tggccatttt cattcagttc atcgaccatt tcttgtgcat gtttctttac
    31441 ttctcgcagt gcttcttccc catataaatg ctgtgatcct acaacaaacc aaaattcttt
    31501 ttctccaata ttcaacataa ttagccactc cttttatttg ttttgtccgt aatacgcatt
    31561 ttttccgtgt tttcgtaaat aatgtttatc taaaatccgt tgaggcagct cttctgaaaa
    31621 tgaattgagc tgtctggtaa ataaattcat cttgcatact tcatctagta cgactgcgtt
    31681 cattaccgca ctttgcgcat ctttccccca agtaaatgga ccgtgtccgt gtaaaagtac
    31741 ccctggaatt gctaagggat caattttgcg ttctttgaat gtttctatga taacttttcc
    31801 tgtttcatat tcgtacccgc tgtcgatttc ctgttgtgtc aaaaatcgag cacaaggtac
    31861 cgatccataa aaagtatctg catgcgtcgt ccccatcgca ggaacatcca atcctgcttg
    31921 tgcccagatc gtcgcccatg tcgagtgagt atggacgatt cctccgattt gaggaaatga
    31981 acgatataaa acagcatgag taggcgtatc cgaagaggga ttgagtttac cttcaatgat
    32041 atgattgtcc aagtccacaa cgaccatgtc gtctgctgtc atctcttcat aactgactcc
    32101 gctaggtttg ataacgaaat atcgtgtttc aggatcaaaa gcgctcacat ttccccatgt
    32161 atatttgacg agtccgtgtt taggcaagtc aagatttgct tggtatactt cttctttcag
    32221 ttgttccagc attttctatc actccttttt cttgcaattg atgaatcgct gttttttcaa
    32281 tgtcgatccc ctcacggtaa cgtctcataa atatctgata gcctgctatt tcttctgctg
    32341 aaggtgtgat ggtgattcct tggtcttgtc caaacacgtc ttctgccaag aagttttcta
    32401 aagacttccc atgtcgatcc atcatatagg ctgctaataa cgcgattccc cacgctccac
    32461 cttctccggc tgtgtccatc actgtaactg gtgcctccat agcagatgct agaatccgct
    32521 gtccaacttc tggtgtttta aaaatccccc catgccccac taaacgatca atttggacat
    32581 tctcagattt caatatatcc attccgattc tcattgcacc aaaagcactg gctaaatgga
    32641 tccgcatgaa attcgctaaa tcaaaacggc tacctggtgt acgtacaaat aatggccttc
    32701 cttcatttac tcctgtaatg ttttcaccgg aaaaataacc ataagaaagt aatcccccgc
    32761 aatccgtatc gccttctagc gctttgttga ataataccgt gaataattta tctctgctta
    32821 tctccatacc caaagaattg gtaaattctt caaaaatact gacccaagca ttgatatccg
    32881 aagagcagtt attcgtatga accatgccta ctagttctcc tgaaggtgta gtgaccatgt
    32941 cgatttccgg atggacattt tctagttctt tttctaatac gatcatagcg aaggcggaag
    33001 taccagccga aatgttccct gtccctttgg ctacactgtt cgtagcgacc ataccggttc
    33061 ctgcatcccc ttctggcgga caaaccggga ttcctggtct caaatttccc gtagggtcga
    33121 tcagataagc ccctgattcg cttaacttgc cagcttcttc tcctgctact agaactttag
    33181 gcaaaatgtg atccaagtca atcgctacgt gcgctttttc gattaactga ccaaaaatat
    33241 ttttcatttc ttgatggtag gttttcgttg aaggatcgat aggaaacatg cctgatgcat
    33301 ctcctacgcc tagtactttc tcaccagtaa gctgccaatg gacataacct gcaagcgttg
    33361 tcaaaaaatt aatttctttc acatgttctt ctttattcaa aatagattga tacaaatgag
    33421 cgatactcca tctctgcgga atattgtatt gaaatttttc cgttaatatt cgttctgctt
    33481 cttcagtgat tgtatttcgc caagtacgaa atggtactag taactgttcc ttgctatcaa
    33541 atgctagata accgtgcatc attgcactga acccaatcga cccgattgtc gtaagattct
    33601 cgccatagat ttcgaatact tctgctgcca tttttcggta actgacgcgc agtcctttcc
    33661 aaatatcttc taatgaatag gtccagatac cattttctag tttgttttcc cactcataac
    33721 taccagcggc aataggagta aaagatgaat caatcaatac agctttgatc ctagttgatc
    33781 caaactcaat ccctaaagat gtacgaccat ctatgacatc taacatactt tgcgcattta
    33841 cttccttcat aatgatttaa ctccctcctt ctcgtcaaac ctgatcaaac cgtcttttta
    33901 gtagcgctta caatgattat tgtatacttt tgtacgtaca tgtcaagcag ttatttacaa
    33961 gaatacaata aaaaacactt gttttgtacg tacataagtt ataaaatgaa attgcttaca
    34021 aaatctgatc aaccgccttc tcgaaattct agaaggagag aatacttatg aaaaaagaaa
    34081 aaaaagtttc ctcatcgttt atttattttt ttggcgcatt tggcggcatt ctatttggat
    34141 atgatattgg tgttatgacc ggcgccttac cttttttaca gcatgattgg ggtttggcag
    34201 gtaaagcttc tttgatagga tggatcacgt cttcggtcat gttaggtgcg atccttggcg
    34261 gctctttatc tggtcttctt tctgataagc ttggacgacg aaaaatgatt cttctttctg
    34321 cacttatttt tatggctggt tctgtccttt ctgcatctgc ccctcacaat ggttcttatt
    34381 ttctgattgc agcaaggatt cttctaggtt tagctgttgg cgctgcatct gccctagttc
    34441 ctgcttatat gtctgaaatg gctcctgcta gactaagggg aagattgtca gggatcaatc
    34501 aagtgatgat tgcttctggc atgctccttt cctatgttgc agattatttg ctaaaagggt
    34561 tgcctgaaac gatggcttgg cgcgtgatgt tagggctcgc tgctgtcccc gcgctgattt
    34621 tatttttcgg tgttttagct ctaccagagt caccgcgttt tcttatgcaa tctggccgct
    34681 tggaagaggc aaaaagagta ttgaactata ttcggacacc aaaagaagct gaacaggaat
    34741 tcgaacaaat ccagctgaat gtcaaacaag aaaagacaac cggaacttct tggcatactt
    34801 tgtttttaga aaaatatcgt tccttagtca ttgccggtat tggtgtagca gtattccagc
    34861 aatttcaagg agctaatgct attttttact atattccgct gatcgtagaa aaagcgactg
    34921 ggcatgcagc gagcgatgca ttgatgtggc cgatcatcca aggaatcatt ttagttgctg
    34981 gttctttgct ctttttggtt attgcagaca agttcaatcg gcgaacttta ttgaaaatag
    35041 gcggtagcgt gatgggactt tctttcatcc taccagctgt tttaggaact gtactggatg
    35101 ctcatacaaa ttcgttgttg attctcttgt ttttatgtat ttatgtcgca ttttattctt
    35161 gcacatgggc tccactgaca tgggtaatcg taggcgaaat attcccacta gctgtccgcg
    35221 ggcgagcatc tggcctagcc tcttctttta attggattgg ttctttccta gttggcttgc
    35281 tttttcctgt gatgactgct tctatgtctc aagaaatcgt tttcggcatt tttgggatca
    35341 tctgcttttt aggggtttta tttattcaag agatcgtacc agaaactcgt gggaaatctt
    35401 tagaggaaat cgaacaaagt gcatctaaaa aaacttatcc taaacgtatt tctatccatc
    35461 attcttaaag aggttgtgac agaagtggtc agcttcaaga aataagaagg aaccgcttag
    35521 tttccagtca caaggaacaa tttcaaaaaa cagcttctca tgtttgatga atattgtgac
    35581 ttgtgaccag ttggtaggag aacaccgttt agtggagagg tggtgaaaga aacgttcagc
    35641 cttgagcgat gacaaaagct ctcacaaaca ttgcgacgag taaccgtagg agcaataaga
    35701 ggcggtaaaa acccgtctct tatttttcgt tttctttcac tgtttcccgt tcaactagta
    35761 caggttcata gagaatattt ggctgtgcct gtcctttttc aatcgttgtg acgatccacc
    35821 gtgcggcatc ttgtcccaac ttttcttttg gatgcgtcaa tgttgtcaat tttttattcc
    35881 ctaaatggct taaagaagaa tcgtctgttc ctacgatcga taggtcatct ggaatatgat
    35941 agccattttc tgtcaactta tctagcatta aactagctat ttgatcgtta tagcaaatga
    36001 tgccatctaa agcttgttct tctttcaacc gttgtactgt tttttcagcg attgtttctc
    36061 gtgtttccgt ggtgaaagta atgatatctt ccgtcaaaaa acgcacatta tactgttcac
    36121 acgcgctgat aaaacctttc atcctgtatt ttccttgaag atcgtcgatt tttgtgatga
    36181 acaacagacg ctgatgtcct ttttttatca aatattctgc tgccataaaa ccgactttca
    36241 catcatctaa acaaatataa gaggcattta actcttcata agcagcattg atcatcacca
    36301 tcggaatgcc ttgttctctc aaagcaacat aaagtgctaa gtttgggtta tactgattac
    36361 ttttcgtagg ctcaacgatc aaaccatcta ccccataagc aatcattttc ttcaaacact
    36421 ctttctccaa ttcgtgatcg ttattcgtac tggctaaaag caatgaatac ccagattctc
    36481 gtaattcatt ttcgattcct cggataatcg acggaaaaat ataatcagag agatatgtgg
    36541 tgatgacacc gatcgttttt gttctgttca atggtgtttt ttctttctta tagctgtcat
    36601 ctacatatgt gcccgaccct ttttctttac gcagatagcc ctcatttacc aacacagcga
    36661 ttgcttgccg aatcgtatgt ctgctgacgt catatatttc ttgcagcttg aattctgagg
    36721 gaatggttgt atttacttcg tattcctcac gaaggatctt ttgttttatc tcttctgcaa
    36781 tatcttgata ttttgccatt tgccttgccc ctttttattt gtacgtacat atatcataac
    36841 gagtctgcaa ggtttattca agcaaaaatc aaggtttctt gtgctcataa ggcatacgga
    36901 acaggttcac caaattctgg aaatccttgc tcattccacg taaagcattg cgcgttcgcg
    36961 tgccgattag gattgtctaa cggatcccct ttttggttct tctctggtcg agcatgatag
    37021 atcaaaatat caaagcaccc gtcttctgaa accgtaaacg agttgtgacc aggaccaaat
    37081 tgcttatttt tttcagaaga aacgaatact ggctccgata atttatgcca tgattctttt
    37141 aataaaagat tcttgtgctc atcagcccac agcaggccca tggcatagtt ttcatctgtt
    37201 gcactaccag aatacgtaat gaatattttc ccattgcgag taaggactgc tggaccttca
    37261 tttactgaaa aaccccgttt ttcccattca tactcaggta tagaaaggag gatctgcttc
    37321 ccctttaacg tccaaggatt atccatttcg gatagataaa gattagaatt cccagtaatt
    37381 tcaggatctt gttgggccca cacataatat agctttcctt gagaagagaa agttgttccg
    37441 tctaaactga aagtttccct tttcgttttt atttgtccct tttctatcca atctgtattc
    37501 attggatcta tatgctgatt ttcaagtacg aacatgcggt gatgatgaga ttcatcacgt
    37561 atagttttat gattgcttgc agcaaagtaa atataccatt ttccattgat ataatggact
    37621 tcaggtgccc agatcaattc actcatcggt ccttcttcat gagcctgcca tacaatcagg
    37681 cttgcagcat gttctaattc attcagtgac tttgcccttc ttaattcaat cacttggtag
    37741 cctggaaccg atcctgtaaa ataataataa ccatctgtat gtttatatac ccaaggatct
    37801 gcccgttgcc gaacgatagg attattataa agtgtcttca tcataacaaa tttcctcaca
    37861 ttttctttat tctaattaca ttccaagagt atttttctat ccctacattt aattcgtcat
    37921 gtttttctat tgaaaaatag tttatttcag gttttatttt ttctggttct tcacgtgtat
    37981 ttttgtccaa cagatcgcta ctggttaatg tgtagtgctc tccttgatct agcaaattga
    38041 aatcatgttc aatcgttatc ttttgttgtt catttgtgcg attgatcaag aaaatagtca
    38101 gctgattgcc gttatctacg atcacactgt ccacataagg aacatttttt atcttttcac
    38161 aatcataaac agggcaaatg atttgttgtt tcaatacttg acctttagca tatttcgata
    38221 agtgttgcat cacataataa gttggattcc tccatgcttg tcctttagca tctgtcagta
    38281 ttaaaggtat tgtgtttact aacaatgatt gacaactaat ctttacgcga tctgcgtgtc
    38341 gtaaaatagc cagccctaat gaaccaaata ataatgtatc ttccatagta aagtaacacc
    38401 aatcgatggg actaccgact tcaaaatccc tatgtctttt ttccggttgg gcatgtacat
    38461 tccattcatc aaacgctaga tacattgttt tctcacttct caaatatcct tttacataat
    38521 cacaagtagc aacgatttct tcaatttgac gatcgaatcg ataagctttt gcaaggtatg
    38581 tcgctgtatc atcacgctca tttttaggag gtttagtcaa gtcttcttca tcatacttat
    38641 caatatagtt atgcaaagct aaataatcta cattctcata agcttcttct aatgcgatac
    38701 gatcccattc agggtacgat ggtaaacgtg gcgtagagct tccaactaag attgtttcaa
    38761 ttgaatcatc cactaacttc atgacttttg aggcttcatt tgctaaccta ccatattcat
    38821 aagcactctt agcccccact tgccatggac cgtctagctc attccctaaa caccataatt
    38881 taacgccaaa aggctttttt tgcccatttt ttattcttaa atcactccag aaggttcctt
    38941 ttggaaaatt acaatattct actaaatttt ttgcttcatc aattcctttt gttcctaaat
    39001 tcaccgtgaa tatcggtttc gcttgtacat gactgatcca tttaaaaaat tcgtggagtc
    39061 caaaagaatt tggttctata cctcgccatg ctaaatcaat tttttttggt cgttgttctt
    39121 tcctacctac agtatccacc caatcataac cagatgtaaa gtttcctcca ggatagcgaa
    39181 tgacacctaa ttctaattcc tttacataag agaggacatc ttgtctaaac ccgtcaccat
    39241 cagctgtttc atggcccggt tcataaatcc ctgtatagac gacacttccc atatgctcaa
    39301 caaatgagcc aaacagccga gaactgatct ctcctaccgt aaaatcctct ctgattttta
    39361 ctttcacttc ttcgtccaac agctttccct acctttcatt tcctatagtc aagataatag
    39421 atacaagttt ccatacgcca ccagcaaaga taaaaataaa aggagggaaa agaaatatga
    39481 gcagtacaaa tcctaataac aatacgcaac aaagaatatt tctcaaaaaa tcaaaaaata
    39541 ctttccatgc actttgttca aaaatttgta aaaatgtgat atctttgttt tcagaaatca
    39601 catatatcca ggataaaaga aacatggaat aaataagtaa aactacatat ccactaattg
    39661 aaagtacgat tgtctgttct tctaataatt gattaacaag aaaataaata atgatcgtta
    39721 acagactaag gaacaagttc ttaccaaaat gttgctttat tccttgaaag aaaaaataaa
    39781 tgatatttcc tgatccctca cctttccatt gcgccattac ataaaaaaca gcacatgtag
    39841 caggcacgat agtaataaca ggcaaacaca cgattaccca gagaatacct aataagatgt
    39901 aatcagaaac cacaatcaac tgtccataaa gtttagtctc aactattttc atcttcctca
    39961 ctctttcatt ccagacatag ctgttccttg aacaaaatat ttctgtgcaa aaatataaac
    40021 cactagtaat ggcagcattg aaataaccgt tccagccatt acaggtccat aaaaagaaat
    40081 gttctgccct gtaaataaag ctaaccctgc tggtaaagtt ctcatttttg aactacttgt
    40141 caatatcaat ggataaagca agtcattcca actattcatc agcgtgaaaa tccctaatga
    40201 taaaagagta ggtttgctca atggcaacat gattttcaaa aaaatattaa actcacttaa
    40261 tccatcaatg cgtgctgcct cttccaaaga atctggtaaa gtaataaaaa aggaacgcat
    40321 catgaagata ccaaatgcag ttgtcatacg aggaatgatc aaacctgcat aagtatcaag
    40381 catgccaagc aaattgactt caataaatag aggaatcatg aagacttgga atgggatcat
    40441 cattgtcagc aaaacgaaat agaacaaaac tgattttcct ttgaaacgca tacgagcaaa
    40501 tgcatacccc gcaagagaat caaagaatac agaagtgatc actgtccctc cagcaaaaat
    40561 cactgtattt ttgatgtaat ctaataaagg gatcgttgtc caaaccttaa tatagttatc
    40621 tagggtatag ctatctgcaa atattctagg cggaaatgag ataatgtctt tttcgtgttt
    40681 aaaagaagaa ataatcagcc acaaaaaagg aaacgcaata accaacaata taactagcat
    40741 actaatcgtt cgaatgattt ttttaccact aactgctttt cgactattca cagtaactgt
    40801 aatcgagctt tctggtgcca tacaggtttc cctcctattt catttgtttt tctttccgtg
    40861 acatgtacac attagtggag atagctatag ttgcaacaat gataaacaaa accgaagaca
    40921 gtgctgatgc atagcccaat tcataaggtg ctgtaaatcc tcgactgtaa atatattgaa
    40981 cagctgtttc tgtcttaaat tgtgggccac cagcggttgc aacatatact tggtcaaaca
    41041 cttgcatcga accaatcaga ttcgttaaaa tacaaaaacc taaggaggga acgatctgag
    41101 gtagcgtaat gttaaaaaac tgctgtcgat ttgttgcacc atccatttga gcagattcat
    41161 acaaagaggg agagattccc tgaatagcag tcaagagaat cgtcatagta acaccaaaat
    41221 tcttccaaac agtcatgatg gctaccgtcg gcatagcaag ggttggatcg cgaaaaaatt
    41281 gagggatcgg caagccgact tgtgaaaaca agtaaggaat cgcccctaca ttcggatcaa
    41341 gcaacataga aaaaataatc gcaattgacg tcaatgaaca aataactgga atgtaaaatg
    41401 tcgaacgaca aaaacgattg aatctggtat ttttagataa tgctactgct gctaataaac
    41461 caacgataat ttgtgttggc gtttcgagta cggtgaaata taaagaatga atcaacgaat
    41521 taactgctcg ttcatcacta aatatacgtg tgaaattttc aaaacttaca aaatttcttg
    41581 atgtaaagaa aatattcaaa tctgtaaaac taatagcaaa tgttcctaaa agcggtatga
    41641 cagtaaataa taataaaact aaaaaagatg gagttaaaaa caaatacgca gcacgatcag
    41701 gacgattcca atatttggtg aatccgttca tatagtccac ctcatctttc attttgataa
    41761 aatatacaat cttggtttaa acgaatctga ataagatatc cgaacaatcg ctgtgatttt
    41821 tgcaataaca cctatctgca aatactaggt cgatcattca gatatctgta tcttgccaac
    41881 agtctatcct gtataaagtt ctgtactatt tttgcagtaa ttgattgatg gcatcatctg
    41941 catcgttcat gagtgtctca ggatcttctc ctgctaataa tttttcaatc agtggattga
    42001 tgatatcgtt attgatcgca gagatttggg taaaacctgg taaatatggc tctgcatagt
    42061 ccatttgttt cgataattca gatacaatag gatcattttt tacttcttca tcttttgcta
    42121 ctgaatccaa gtaggcagga aaaccatttt cgatactcca ttttttacca atttcagttg
    42181 tattccaata tttcaaaaac tcatagacag cttctttttt attattatct gtacttgccg
    42241 gaatggcata tcctacgcca tctaaaatag ctgttttttt gtctgaatca ctcccttgtg
    42301 gtaaggtagt cacattataa tcaatctcgt ttgctttcaa tccgttattt aaccatggcc
    42361 cattgatttc aatggctagt tgaccagcat tcattaaatt gtccatttcg gcgccagaaa
    42421 tcgattctgg accagtcttg tctacatgga tcattttttg gatcgctttt aacgttttaa
    42481 ggttctcagg agacgcaaat tcagacttag tataatcatc gttcactaat ttaccgccat
    42541 ttgctaacat ccagttatat aacggtgcag ttccatcctt attgaagaca aaacctgata
    42601 catttttact actatcagtt aattttggtg ccatttctgt caattcttcc caagtagtag
    42661 gtggttgttc agtatctaat ccagcttgtt taaacaaatc attattccaa tacatataca
    42721 tgccttgtac ctgcatgggg acaaaatact gtttaccttc tacttcacct aattcaatag
    42781 ccgtttcttt aaaatcagat ttatctactc catcatattc ccagaaatcg tctaatggtt
    42841 gtaccgctcc gttttctaca tactgtgcaa aatctggata atttaatgca ataaagtcag
    42901 gtgctttttt agaagaaatc gctgtaggaa gtttttcatt caaattggcc caagtcatga
    42961 tatccatttc aattttgatg tttttatcat ttgttttatt aaattcatta acgatttctt
    43021 gcaatatttc accatcagat gcagtaaacc cattccaaaa agtcaaagtc acttctttat
    43081 cagaatcaga ttctgctgaa cttccgccac cgcaagctcc taaaatacct gttgcagcta
    43141 ccaacaaaga gccaattaac cattttttac ctttcatttt ttgttcctcc ttcatctgat
    43201 aaacgacatc tgaccacatt ccaagatagc ggtttcaata aacaattgat tttcccatct
    43261 tcagctttta catttgttga atgacttggt tttacttgtt cacttttagg aagattagtt
    43321 gcttttgtat caaatccact catttcagaa aaatcaatca ctccctctaa tgataattca
    43381 gacaagtgaa cagttagatt catttcttct tctccacggt taactgcaaa tatgacaagt
    43441 tctttgtcat ttattacggc tatactttct aaatacggca cttcttcaaa atcttccgtc
    43501 gcatagtttg ctgaacgaat ttgtgggctt aacacctctc cttgtccata cacagcagct
    43561 tgcataaatg gataatagat cgtctgtctc caagcttctc cgtttgtttc tgtcctgatt
    43621 ggtgcaataa catttaccaa ttgagcaaga caagcaatct ttactctatc tgcatgtttc
    43681 aataaagtga ttaatagaca gcctaatagt aatgcatctt caaaattata gttatcttct
    43741 agtaaaggag gagcaatctg ccaaggcgct gcttgctcat cttgttccgc tgaatgatac
    43801 caaatattcc attcatcaaa ggacaggttg atctgcttct tactatgttt cttcgccttg
    43861 actgtatcgc acatcgctgt cacttcagaa atgaaccgat ccatgtctaa ggaacgtgca
    43921 agataatgag aaagatcatt tttttggttg ccataatatt gatgcagaga caaataatct
    43981 atttgatcgt acgcttgatc aagaactgtt aactcccaat caccaaatgt aggcatttgc
    44041 cgattcgaac ttccgcataa aaccacctca attgaatcat ctacgagttt catcgcctta
    44101 gccgtttcct ctgctaaccg tccatattct tccgctgttt tatgtccaat ctgccaagga
    44161 ccgtccattt cattccctaa acaccatgtc ttaattccaa aaggggtctc ttgaccattc
    44221 gaacgcctta aatcactcca ataagttcct ttaggaaaat tacaatattc gactaaatta
    44281 cgagctgcat ctactccacg agtacccaga ttgacagcca tattgatttc ggcccccaca
    44341 tgctgacacc agttataaaa ttcatgaatc ccaacttcat tcgtttcaat tgtccgccaa
    44401 gctaaatcca accttctagg tctatttgct atgggcccaa tactgtcttc ccaacgaaat
    44461 cctgacacaa aatttccacc aggatatcta atcatgggaa tgttcaattc ttttaccaag
    44521 tgaatgacgt cttggcgaaa tccactttta ttagctgatg gatgatctgg ctgataaatc
    44581 ccttcatata ctgctcgtcc catatgttca ataaatgaac tgtacaaccg cggatcaact
    44641 ttgcctacac gcttgacact actaatcaac attgtggctt ccataactga cctcctttaa
    44701 tttatacatg aataatacac ctcgttgaaa gcgttgtcaa taatataaat gttaaactta
    44761 cttatttttt atcgatttta attaaccaaa atattaataa tgattaaaac aaataattta
    44821 atataacttt tattaacaaa tatgttaata tataagaaaa accttttaag aggtgatata
    44881 tatgcaacta caaataaacg aagaatcctt accggtatat gaagcattag ctagtaaaac
    44941 aaggatcaag atcatccagc tactttccaa aaaaaagatg aatgtcaaag atttggcaaa
    45001 agaacttgga gtgagcagtg ctatcacgac gatgcacgtg aaaaagctag aggaagcaaa
    45061 catcatcaaa acagaaaaag taggacaaca aaaaatttcg agccttcgtg tcgataaaat
    45121 cgatatcagt tttccagaaa aaatattcaa tgcttttgat acgaaagaaa catcgatacc
    45181 tattggtcac tatacaaatt atgcaattga accaacatgc ggtttagcta cgatccatga
    45241 ttttatcggg aaagtggatg aaccacgcta ttttatggat cctcgaagga tggatgcccg
    45301 cattctctgg tttaccagcg gatttgtcga gtatcaggca cctaattttt taaatacaga
    45361 ggatacattg gagatgttgg aagtttctgt ggaaatttct tctgaatttc ctttttcaaa
    45421 tgacaattgg ccttcagata tcactttcag cctaaatggt gtggagctag gaacatggac
    45481 gagccctgga gattttgctg atatccgcgg taaatatacg cctgattggt atccagataa
    45541 tcttaatcaa tacggcttgc tgaagacgat ccgaattacc aaacacttga cgaatatgaa
    45601 tggagaaccc ttgtcaaaca tcacgatcaa cgacatccct aaagagcaag acacttggca
    45661 tttgcgaatc gaagtgaaag acgatgccaa acatgtgggg ggctgtactt tatttggtaa
    45721 aggattcggt aattacgatc aggatatcaa attaaaagtt tactacagct gatatttttc
    45781 ttttctattt tataaaacaa aaacagccaa tccctatgaa gggacggctg tttttgcttt
    45841 attgttattt ccagaaatca tccaaaacgg aaattggcaa atgtcgttta tgttgtgtct
    45901 tgttccacca tgcttcgatt ttttcttgtg cttgagcggg aacttctttt ccttctagat
    45961 aatcatcaat ttcattatat gtgacaccta gtgcgacttc atctgcaatc attggtttcc
    46021 catcttcaag atctgcagtc ggaatttttg tgtataattt ttccggtgca ttcaatgctt
    46081 gaagcaattg tttcccttgt cgtttgtcca aacggaataa tggcaaaatg tcggctccac
    46141 catcaccaaa tttcgtgaag aatccagtaa tgttttctgc agcatgatct gtccctaata
    46201 cggctcctgc tctttctcca gctacggcat attgcgtaat catacgttga cgtgctttga
    46261 tatttccttt gttaaaatcg ctgattccca cacctgcttc gcttaatact tgtacttgtg
    46321 catcaacagc tggtttgata tttactcgca gactaacatc gggctggata aagtcaagag
    46381 cagcttttgc atcttcttca tctgcttgtt caccataagg aagacgaaca gcgataaatt
    46441 gatagttttc atcttttgtt tcttcacgca tttcttccat cgttagttgc gccaaacggc
    46501 ctgctaaagt agaatcttgg ccgccgctga tacctagtac aaaagtttta agaaacggat
    46561 attttttcaa atatgctttc ataaagtcaa tacttttacg tatttcttct tttggatcaa
    46621 ttgttggttt gacacctaat tcattgataa tttctgtctg caggctcatt tattctcctc
    46681 ctatgcttct gtatgaagtt ctgctacgct ttttttcatt cgttccatga tagccatttt
    46741 atggttccag cattcagtag acagatcgac tgggtatttt tgcggattca agtctcgttt
    46801 atactcttcc catagtgaat ctagattttc ttttgtatat tctttgattt cgtttagtgc
    46861 aggtaattcg taaactcttt ttccttcgat aaagatatct tgtaaaactg ggcgagctgt
    46921 aaagtctcga acagttttat tgataaacgt atgcactgga tggaacatga agatagcttc
    46981 ttcttctcga gggtcttcat cccataatgt cacgtagtct ccttcagatt tcccatcaaa
    47041 atttcgagtg atgcgccaca cttgtttttt ccctggagta gttacttttt ctgcattgct
    47101 tgataacttg atcgtatcca tcatttttcc ttcatcatct tcgatcgata ctagcttgaa
    47161 tactgcacca agggctggct gatcataggc tgtgatcaat tttgtcccaa caccccatac
    47221 atcaatttta gctttttgca tctttaaatt caaaatcgtt gcttcatcca agtcattcga
    47281 agcatagatt tttgcttccg taaatccggc tgcatctaat tgttcccgaa cacgcttaga
    47341 tatataggcc atatctccac tgtcgatgcg tacgccttgg aagttgattt tgtcacctaa
    47401 ttctttcgcc acgcgaatcg cacttggcac acctgatttc aacgtatcat aagtgtcgac
    47461 taagaacacg cagtctttat gggttttagc gtaggccata aatgcttcat aatcatttcc
    47521 ataagattgg actaacgaat gagcatgggt cccgctagct ggtatcccaa atatcttccc
    47581 agcacgtaca ttgcttgttg catctgctcc gccgatatat gccgctctag ttccccagac
    47641 tgctgcatcc agttcttgtg cccgtctagt tccaaattcc aatagtggat cttctccgat
    47701 gacagatttg atacgagctg cttttgtcgc gatcaacgtt tggaaattga ccatattcaa
    47761 cagtgctgtt tctactaatt ggcagtgcgc taaaggccct tccacttgga ttaatggttc
    47821 atttgcaaaa actagttctc cttcacgagc agaacgaact gttgctttaa attcaaaatt
    47881 ttgtaaatac tccaaaaaac cttcaggata gacttctaat ccacgcaagt aagcaatatc
    47941 ggaatcattg aacgtcagat tctctaaata attgactaac cgttccaatc ctgcaaaaac
    48001 cgcgtatccg tggttaaaag gcatttctcg aaagtaacat tcaaaaacag catgtaaatc
    48061 agctcgacct aattcccagt atgtctgcat catattgatt tgatatagat ccgtatgcag
    48121 cgtcaaactg tcgtctgggt aagtattatt catcttgtat tactcctctt ggcaagtttt
    48181 catttcctac ccattataac aaaaaaaatt aataggaaaa gaaatcttcc attttttctg
    48241 aaaaagcaca tgttttacgg atatttcttg ctaaaaatga actccatctg ctaaaactat
    48301 cctttcttat tggtatttaa cctaaacgag aaagaatact tattttattc aatgtatccg
    48361 agacacgaag aaattagcgt tttctatttt tcttacttga taaaataagt agattttcct
    48421 actacaatcg tagccgttaa gataccgatg attgaacaaa tataaccaaa tgtctctaca
    48481 acataatcaa tggcaaaagt ttttgaagca ataatacgtc tttccttttt tgcttcttat
    48541 ccccaaagaa acggatataa cttattatgt attgaatttt tttggaaagg cgaattatga
    48601 caagatatac cttgcgttga aaaaactcgc ggaaaaaata tcacacgttt ttaaaaagat
    48661 acaaagtaag aaagcagctg tcaatagaat ggaaacaaaa atggcgagac atcaatcagt
    48721 ctcgccttgt tttttattgt atacattaga tatggagcag gttaccataa agatgattat
    48781 tagtcgttat taaatagaat ccttcgtatt tttttgttct atcactacaa tatctttatt
    48841 tggacgtttg aattgtattc cggcacgttc tagctcctgt ttcactccta catggacttt
    48901 gacatcttct cgtgtcattt ttaaaacgtg atgaatcgca tagtcgattt cttgccttgt
    48961 gatttttttc ttcgtattga tcatcacgac ttgcttttta tcatatgtgt ctaccaatag
    49021 cagttgtgtt cgatctaacg aaaataattt gtaataatta atagccgtat tcccaaaaaa
    49081 gaattccact actttaggat aaaatgtctt aatatttaaa ttactattca caggtgtttc
    49141 gattagcttc atctcattcc tcttttctat atagtcgatt tagatccaat cttaagttat
    49201 gttaccatct ttcctagcat taaggaatcg gttacaattg aacctttaaa gacaaaagga
    49261 tttacctttt atcacttaat agtataacat aatttcgtga gaaaaaaata caattttttc
    49321 ttgaccatct cgttacgtaa aggattatgc ttacatttgt caggaggaaa aataatggag
    49381 tacacaataa agaaaatgtc agagatatca ggagtcagtc cgagaacctt gcgcttttat
    49441 gatgagattg gactgctaaa accagctagg atcaattctt caggttatcg gatttatggc
    49501 aaaaaagaag tcgatcgtct gcaacacatc ttattttatc gaacaatggc attcaagttg
    49561 gatgatatcc aagaggtctt agacaatcca tcttttgacc accagaaagc tttaatcaag
    49621 catcagcaaa tgttgctgga aaaacgggca caaatcgata ccctcttgac aactgtccaa
    49681 cagacacttg atatgtacga aggaggaaga aaaatgagtg acaaagaaaa atttgaaggg
    49741 tttaaacaac aaaagttgaa agaaaatgaa gaatcctatg gacaagagat tcgacaaaaa
    49801 tacggaaaaa aaaaggttac agcatccaat gaaaaatgga tgaatatgga taaagaaacg
    49861 tatgaagaaa tgcaaactgt tgaaaagcag atgttatttg atctcactct ttacttaaaa
    49921 gaaccagaag atgaaagctt agcagaccgt atcttccaag ctcataagaa gtggttctct
    49981 tacagctggc ctgaatatca gccaaaagcg cataaaggac taggaatgat gtacgaagcc
    50041 gatgaacggt tcactgcata ttatgacgag cgaagtggtc atggtgcagc aaaagcttta
    50101 aatgaaatta tccaaaaaaa ggcgtaatct tttacatgat ccaacaaaaa agtacacgta
    50161 aaatgaccta ataacaaaac agcagacacc caagttagat ttttatgcta acttttggtg
    50221 tctgctccat tctttcacaa gttttttcat cctactttaa atatattcat cgttgaataa
    50281 acgtataagg tattttcact ttatcctttt tttctgtaag aaacaattga aatgccattt
    50341 ctccgcaagc ttccagatgg tggtcgatgg ttgggaaacc aagaagctcg ctgatcagca
    50401 aattatcgcg tccaatcacc actggtgtct tatcttgcag atagctttgc aagatgcctg
    50461 ctgcaaccat atccgaattt gtaaagacac catcaactcc aagctcttta aaaaagaagc
    50521 ctgcttgttt tcctttctct gcatctatac aatcccaaaa aacaagtgat tcatcaaaag
    50581 atggaaaaag ttgatgacat agctgcaaag tgatttttga gttataactt agcctgccac
    50641 ttcgccccaa cgtgatgccc aattgcttta taccttgctg cttcaaataa gtaagacctt
    50701 ctgtaattga ttgttctcga tcaataaaga cacagcttgc ctgttttccc ttgatttctt
    50761 cacaaaaaac tagaggtcca tatttttgat atgccaataa atcatctagt gtattggcac
    50821 gagacgtaat gatcaatcca tcgaaagctt tagctgcaaa ttcttcaaga taccttcgct
    50881 ctttttcttt atgatagccc gtcggtaaaa gtgtcacttt gtaatcataa tcaaacgctg
    50941 cttcaatgat cccactgatc aactgatcaa aatacggatg attggtataa ggcagaatca
    51001 ctccaatgct tttcgttttt ccataactta actgtctggc atttgcatta gggatataat
    51061 cgacttcttt catgaccgca agtatttctt tccttttttc ctcatctaca tatggatgtc
    51121 cattgattac acgtgaaata gtagaaactg aataacctgt gagctttgcg atatctcgaa
    51181 tgtttgccac tgctgcttcg ccacctatac aaaaagattt tcttgtaaat agtctaccac
    51241 agctggaccg cattcaaaaa actgatacat ttttccttca tgcgaaaaac tgatccgctg
    51301 cccgccccaa tatatatctt ttgtcaaatt ttcgatttca ttcattggta tttcttttac
    51361 tatattccta ttcaaccctt gaaaactgta caacgttaat acggatccct tgaactcgac
    51421 aaacgcataa aaataattgt tcgttgtgcc atctgttatc tctacataag tattcattgc
    51481 aagccctcct tttgatactt tgagtatagt gtatgaaacg cgtctcatag caaggagttt
    51541 gttttgccaa agaaaagttt tattcctctt tcattattac caaagtctct tattttaaat
    51601 tgaatacatt ataatgatac tgtaaatata tctttattat tagtgaatgg agtcaagtcc
    51661 acaatcttgt gtaaaagttc aacctcatca aaagcacaat gttttgcgtt gtgaacctaa
    51721 ctgccatgcc tttctttctg aggagagagg tcaggagcag tagcgcagcg actcttgatg
    51781 tctctcctca gaaagaagag actcgcacag gttcatggtg ctaaatgtta ttcgaattaa
    51841 attctaaata ttttcgtgga gaagccagcc actcagtgtc tttttccatc aaaagtgttc
    51901 caattatccg agtgatggaa ttgatatttg gaaaaatccg aatcactcgt tctctccggc
    51961 gaatctcttc gttcaaccgt tccaacacat tggttgtacg aatccttcgg cggatatttt
    52021 caggaaaaga caaaatttga atagcatctt caaagccgtt ttctaaaatg tcgcaagcag
    52081 cacttaattt tgaatcacaa ccatattttt ccaagaagtg ctcttttctt tcacgtgtca
    52141 attcaagttc agaggcttta aagatactct tcaactcgtc ttttacatca gaagaaactt
    52201 tctttggcag cttatcaaaa atatttctca aaaagtgggc ttggcatctt tgccagctcg
    52261 cacctaaaaa gttcttgcga acagccttaa ctaacccttt gtgtgcatca gaaatgacca
    52321 gttttacacc gaacaaacct cgttgaacga acgattgaaa aacggtatcc caattatctt
    52381 ccgattcgcc gtcttgaaca tcaaaaccaa gaatcgtacg tcgtccatca gaatcaatgc
    52441 cgataccaat atatacacct tttgagacaa tacgatgatt ttcccttact ttcatataca
    52501 gggcatcaca catgaaaaat ggatattccg agccttctag gctccggttt ctccactcaa
    52561 aaacaaatgg atccaactgt ttcatcagat ttgaaacgaa tgatttagaa acagtcgcac
    52621 catccgttag cagttcaact gttttagtta ctttgcgagt ggagacacca gaaatgacca
    52681 tttcaatcat ggcagcgatc aaagcttttt cgcttcgctg atatctttca aagatagtag
    52741 gactaaactt accgtctctg gttcgtggaa catgaagttc taacgtacca attcgagttg
    52801 taaacgaacg atcgtagtaa ccatttcttt gtgattgccg ttcttctgaa cgttcatagg
    52861 catttgcctg aatatattga tttcgttgtt cttccatcag ctgattaaac atgatagtca
    52921 taagtgtttt tgcggtatca ttcgcaccgc tttcgtttat aattgcttga atttcttcgc
    52981 tttcaaaagt aaaatgtact tgagtcattg atatcagtcc tattcgagta tgttttctgc
    53041 aaaaaacatt gtactcctaa ctgagatcaa tggctttttt gatgcttcac ttttacacaa
    53101 ttatatggac gtaatcgtga atggatattt acttttaata gataaaatat tataactatt
    53161 aaaaataact attttcatta taattacaca caaaaaagag cgtgggacaa aagtaaagaa
    53221 cacttttgtc tcacgctcta aacacgtata aacagcggaa gcagaagcaa ctccttcgga
    53281 aataagctga aattcacaaa aatttgaaaa gcaatttccg tgaatttctt cttatttctt
    53341 ggagttaaac gcttctgttc cagcctcttt ttatctgtct ttcactaatg atcagttgta
    53401 gataagcaag ttgctttttc ttacatcata ccgcccatca ttgatggatc cattgcagga
    53461 gctgctggcg ctgctggttc tggtttatct gcaacgactg cttctgtcgt taataacaag
    53521 gcagaaacgg aagcggcatt ttgcaaggca gaacgtgtta ctttagttgg gtctacgata
    53581 cctgcttcaa ccatgtttac ccattctcca gtagcagcat tgaaaccagt gcctagttcc
    53641 acgttcttca atttatcaac aatcactgat ccttcataac cagcgttttc agcgatttga
    53701 cgaattggtt cttctaaagc acgaacaacg atcttgatac ctgttgccac gtctccttct
    53761 gcttctacag cagaaacttt gctgataacg ttcactagag ctgtaccacc acctgagacc
    53821 attccttctt ctactgcggc acgtgtagca ttcaatgcat cttcaatgcg taattttaat
    53881 tctttcaatt ctgtttcagt agctgcacca actttaacaa cagctacacc acctgctaat
    53941 ttagctaagc gttcttgtaa tttttcgcgg tcaaaatcag aagttgtttc agcaatttgg
    54001 tttttgatca attgcacacg agcttcgatt gcttcttttt ctccagaacc ttctacgatc
    54061 gttgtattgt ctttatctac aacaacttta gaagcatttc ctaagttttc gattgttaca
    54121 tctttcaatt ctaaacctag atcgtcagta attactgtac cacctgtcaa aatagcgata
    54181 tcttcaagca ttgctttacg acgatcgccg aaaccaggag cttttacagc tactacgttg
    54241 aatgttccac ggattttgtt caatacaaga gttggcaatg cttcaccatc tacatcgtca
    54301 gcaataatca acaatggacg tgattgttgc aagatttgtt ctaacaatgg cagaatatct
    54361 tggatgttag agattttttt gtctgtgatc aagatatatg ggttttctag aacagcttcc
    54421 attttgtcgt tatctgttac catatattgt gataagtaac cacgatcaaa ctgcatacct
    54481 tctacaacat ctagttctgt ttcgatccct tttgattctt cgattgtgat gacaccgtcg
    54541 tttccaactt tttccattgc atcagcaatc aaatgtccaa ctttatcaga acccgaagaa
    54601 acagctgcta cttgtgcaat ggcttctttt gaatcaacaa cagttgagat attatgcaat
    54661 tcttcgactg ctgcttttgt tgccaattcg atcccgcgac gaatgcctag aggattagca
    54721 cctgcagtta cgttttttaa accttcgcga acgattgctt gtgtcagaac agttgctgtc
    54781 gttgtaccgt ctcctgcaat atcatttgtt tttgatgcta cttctgatac aagttttgca
    54841 cccatgtttt caaaatgatc ttctaattcg atttcttttg cgatcgtcac accatcattt
    54901 gtgatcaatg gagagccgta agatttttct agtacgacat ttcggccttt aggtcctaat
    54961 gttactttca ctgtatctgc taatttatct accccgcgta acatagctgc gcgtgcgtct
    55021 tctgcaaatt tcaattcttt tgccatgatt gattcacctc attgattatt tttgattatt
    55081 tttttatctt ccaaatttac tcaacaatcg ccatgatatc ttttcctgcg acgatcagat
    55141 attcttttcc ttcgtatttt acttctgtac cggcgtattt ttcgaacata acttggtcgc
    55201 ctgcttttac agcggcagga actttttcac cgttttctaa caaacggcct tcaccgactg
    55261 caacaactgt cccagtttgg ggtttctctt tagcagcaga agctaaaacg attcctccga
    55321 cagttttttc ttcttctttt gcgacttcga taatgacgcg atcacctaat ggttttaaca
    55381 cgataaatcc ctccgataac tcttttttta atctgttagc actcttaacc ttcgagtgct
    55441 aacctacatt tttaataata ctcattctcc ctcttttttg caagctttta aatcaaagaa
    55501 aagaaatttt attctgacag ctgcttattg aaattatgat ataattgaaa tcaatcgata
    55561 taagcttgcc ttgcatgctt gttatccctt aaaaggagga attacatgtc tttaaaaaaa
    55621 tatagcttcc tcagcatttt ttgctatggt ctaattttta tctcaccctt gatcctagct
    55681 tcgataggac ttgtaaaatc tacgtctgaa ttgatcacag caactgcagt catgtatata
    55741 ctaggcgccg ttgtcctagc tattttttat tttaagcaac gtgaaccctt agcgatcgaa
    55801 tcagcggtaa aaaaagcttc tgctccaaag attgttattt atggattggt tggaatcttt
    55861 gtcgcattga ttctgcaaag catcgctgtc atgttagaat ctgtattgtt tggcaaagca
    55921 accccttccg agaatacaca aaatatcatc cagatgatca tggaagcacc cgcttttatt
    55981 ttggcgacaa ctgttgctgg acccatcatg gaagagttcg tattccgccg cagtatactg
    56041 gggatcatca gtcgatattc aaatttttgg gtcggagcag tgatcagctc tttgctgttt
    56101 gcgtttgctc ataatgatgg tcatctgcta atctactttt tcctaggttt cttcttcagc
    56161 ctagagtaca aagcaactgg tcggatctgg acatcgatga tcacacacgt aggaatgaac
    56221 acattagttg ttcttgttca attagctgtc caaaaagggc tagtataaac atgactataa
    56281 atactcctgt catcctagat tggtgatttt cccaatctaa agcgacagga gtatttttgt
    56341 tttttagtcg ttcatctttt ctgctgctcc atcatttttt gtctgtcttt cctttttgca
    56401 gtttgcagaa tctatttttt tcaaaaatgg gaaataaatg aaaaaactaa ttgacaacac
    56461 gataccttgt aaaatcgctg caggcactcc gcctactaag aaacccgaga ggatcggcgg
    56521 tgttgtccaa gggacttgga ctgctgtaaa taaaggaacg aatccaaaat ataaagcgga
    56581 ataggtaatc aatcctgaaa cgaccggtgc gaaaataaaa ggaacagcca tcatcgggtt
    56641 catcacgatc ggtgtggaaa aaataattga ttcattgatg ttgaaaaagg ctggtaaaag
    56701 agcaagattt cctaattctc tgtatttctt cgatttcgca aaaaccgtca taaaaaatac
    56761 agcccctaac gtcaatccag cacccgtaac cgtcatgaac tgatcgagaa attgctgagt
    56821 aacgatatgt ccgccatttg ccaccgtcaa ctcttgtcct gaggctaaga tttctgcatt
    56881 cgctaatgaa ttcgcttgca agacgggact aaccaaccca ttgaccatcg cagatccatg
    56941 aagaccaaat aaccataaaa acggaacaaa aaaacctaat aaaagcacac caaacaacgt
    57001 gtctatgaac gattgaatgg gaagctggac atatgtccat actccttgaa caacagtcat
    57061 tctgaacaac tgttgaaaaa ccgctgaacc gataagcgta aaagtgacta taacaatgat
    57121 tggaatcaat gaagtaaaag actgccaaac attttcaggt atcttctctc tttgcttcca
    57181 ccctttcgca agtcttacta aacgtgcata gccccatccg atgatgagcc cagttgtaac
    57241 tgctcctagt atcccttcag cacctgtcca gacagtatct atctgagtat ttagtggctg
    57301 aataagcagt agataagcag acaaggctgt tatgccgact ggtaagcgat cgatgtcttc
    57361 ttcttttgca taagcgtatg caatggcaaa aacgatccaa aaggctatca catcaaatgt
    57421 agcctggtat acttgtgtaa aatacataga aaatcctgtt ttgtctatcc actgagcgat
    57481 gtaatccaca ggaaaatgag caaataagaa aaacagcgag ccaacaataa ttattgggat
    57541 cgtgtaagtc attccattac gcaaagcagt taagggtcta agattcacaa cagatgttat
    57601 cattggtaaa aaggcacgat ctaaccactc tttcatttct tatctccttc aacttctatt
    57661 tttatcaatc agttttctta atttaactat acccaatgat cacgaaccat gaaaggaatg
    57721 cgcactgagc ttttcaaaaa tcggattctt tgacgaatat tcagcaactg cttacaatgg
    57781 acaggaataa aaaaggaggt tttttatgaa aattgcactc attggagcta ctggccatgc
    57841 aggttcattt attttagacg aagcattgtc cagagattta gacgtgacgg caatcgtgcg
    57901 tcatccagaa aaattgccat cagatgttcc gtttatacaa aaggatttat ttgagttgac
    57961 aactactgat cttgcatctt ttgatgttgt cgttgatgct ttcaacgctc ccaaaggaaa
    58021 agaagaaatg catcaaactt ctttgtccca tttgattgag atgcttgaag gaaccgatac
    58081 acgcttaatt gtcgttgggg gtgcctcttc gttattctta gatacagaaa agacgactcg
    58141 tatgattgac caagtaccta aaaatgcacc cttctatcca acagctttca atatgtacga
    58201 aggattgtta aaattgaaac aatctaaaaa tctacattgg acttatatca gtccagcttc
    58261 tttatttgat ccacatggca aacgtacagg tacttatttg ttaagtgatg attatttaca
    58321 aagaaacgct gctggcgaca gtacgatcag catggcagat tacgcgattg cattagtaga
    58381 tgagatactg gatgagaaac atgaaaagca gcacatcagt gtagtcagtc gataatacgg
    58441 ttattttttt aatatgctaa aaaaataaaa atagaagata aaaccaaccc tgattgattt
    58501 tatcttctgc tttttatatt tttattgatt gaactgttta aatcgaaaat agctcagttg
    58561 cttcatctgg atctgtatca tagatttcaa aatcatgtcc aacgccaaaa tctttttctt
    58621 tcaaaacatt ttccattgtt aaatgagcaa gttctttcat attgttttcc ttgctaagat
    58681 ggccaagata gattcgtttg gtccgattac cgatcacatc agtcatgaca agtgcaccgt
    58741 cttcgttgga aagatgtcct ctatctccta aaatacgctg ttttaaattc cacggataag
    58801 gtcccatgcg taacatctca agatcatgat tactttccat caaataacca tcggcatttt
    58861 caatgacacc tcgcatataa tcactgcaat atccggtgtc cgtcaacatg acgaatgacc
    58921 gattttcttt atggaaacga taaaattgcg gagccgctgc gtcatgggag acgccgaaac
    58981 tctcaatatc gatatcgccg aatgttctga ctttccccat ctcaaataga tgtttttgtt
    59041 ccactggtac atttccaatc agtggttcca tcgcttccca agtctgctga ttggcaaaaa
    59101 catccaaatg atacttacga gccaagacac ccacgccatg tatatgatct cgatgttcat
    59161 gcgtcacaag tattccatct aattggtccg gtgtccgatc aatttgagcc atcaaggaag
    59221 tgattttttt accgctaagt cctgcatcga ccaaaagccg ttttttttca ctttcgatat
    59281 aaagtgaatt acccgaactt ccacttgcta ggatactgat tttaaaagct ggtttttctt
    59341 cagccatttg actcctactt cccttcttac tttgtaaact tttttcatta tactcgcttt
    59401 tttattgatc ttccactttt ggtacagtat tgtttgtcac gatcgtattg ctgaaagcgt
    59461 tgacactctc aatttggacc actttatcgc ttgtttcgat cgctacatgc caaacaggaa
    59521 catacacatt tttttcccgg actttcagaa tacaggaata agctagctga cgccataaaa
    59581 tttttgatcc tcttggtatt ttgttattga tgtacaatgt attgattgct tcctcttccg
    59641 tatacagttc cgtcttttcg cgcagctgct cgatatcaga taaatgcgtc tgcatgtatt
    59701 tcgtgatgct ataatcatta tcgttttttt ctaaagaaag aacgattttt gctgtatcat
    59761 cattgaatgg gatgccttca tactcttggc ttgcttggat ttctagtggt tcttcttcta
    59821 aatgagaaaa gtttttcgtg tacacataat cattaccgaa taaaatattg tctttgttag
    59881 ataaaaaatc ttccaaggaa ctactaattt tatctggatc gatcacgttc ttttcagcat
    59941 ctgaactgga taaagatttc gtcaaaacat caccgtctaa aaacacacca cttgccagca
    60001 agccgctttt accagccttg cgataattcg ctaatgaagt gctaagattg gtctgctccg
    60061 cgctaagata atagccttgc ttgttctcag acgaaatctt ccccgtgtaa gtgatatcgt
    60121 cgctagccag acgttgttgg attgattcag tctgattaga gcgaatgaca ttgttttctt
    60181 ggtataccgc gtcatgataa ctgctgaata aaaacacatt caaccctaaa aaagcgacga
    60241 aaaatatcca ttcgattttt ttgaaatcca attaattcgc ctccaatcca ggtaattttt
    60301 ccagaaggtc gttagctgaa taccagttat ctttatattt cacataccat tcaggtaaga
    60361 gatcaacgac acgattcgtt tctttgacat tttgccatgt atagccaacg atcactgaac
    60421 caatcttgtt gctatctgct cccttagctt ttaactgctg gatgatttca ttcgtacttg
    60481 caagttctac ttcttcgtct gaaggtatcg gcacttgaat cgtattcaaa ctggtttgga
    60541 tattcacttg cacagaacca ttcgtttcac cgccaacttc cacaccaatc tgtcctctgc
    60601 tgtctgtccc aaaaaccggg aagccttcca cgaatatccg ataatcgatc gtattgtcac
    60661 tgcggtcaaa atatctgagg ttccctaaag aactgcctaa tttgctaata taggggaaac
    60721 tttcagaaaa tacactagca ttttcttttt tctctggcag ctctccacga aaatcaacta
    60781 cttgcgtttc cgaatggata gacattgttt cattcccacc gtaaaaaatc aattcattgc
    60841 tttcatcatt atttttcacg ctatctggtt tctggaaaaa agcatttcta aaaagtgtat
    60901 agggttgctg tgacaggata taactatact ttttcagttt gatcgaatgt ttcgtagcat
    60961 attgcgtgtc gatcgtcgat ggttctgttg tcatttctgt ccattttgtg tcttttgctg
    61021 ttaattcttt attgatatct gtccaattca acgaaatatt tgcttcatat acgagatggt
    61081 tcgaataatt caaaaatcgg atttttttat ttccttgatc gaattgtatt ctagtaaaat
    61141 acatattttc gctttctttt gccaagttcg acacatctaa atctaaatca aatgcttcct
    61201 tgtattctct tagcaaaaaa ggcgcattat aggccaactc aaaaccagag ttcagatttt
    61261 tcacctgttc aaactgtgat ttgtctcctg aaactacttc agaaagcttt ccaaatcttg
    61321 ctttatcgat gatcaattgc aatcggtaca cgagattttc actgttggtt tcttggatca
    61381 ttcccgaatg gtgccatgta acatgaagcg gaagaaagac ttcggaagtt tttcgattgc
    61441 tttgagactc gatcatttga gtgtttgtct catctacgtt gattgaactt tttcctgctg
    61501 ggctcagcca gattgcataa gagaagtata tgcttaacgc aaccatcaaa atgagtccta
    61561 tacgaacgac tttttcagag attctcattc ccaccattcc tcctcataag gttcatatgg
    61621 cagcgagata taaaaagtcg acccttttcc ttccttgctc tctgcccaaa tagcgcctcg
    61681 atgcgcctta atcacttctc tagaaatagc taatccaagt cctgtcccac cttgtgctct
    61741 tgcacgtgct ttatctactc gatagaaccg ctcgaacact cggttcagat cttttttagg
    61801 aatccccaat ccttgatcgg tgatacttaa aaccacgtta ttatgcgttt ctaataagcg
    61861 acaagtaatc gttcccccat ctggtgaata cttgatcgcg ttattcatga tattatcgac
    61921 tacttggatg attttatctg tatctacttc gacccaaaga tctctttgcg taaattcacg
    61981 acggatctta taattttttt cctgatttcc caccatcata tcaaaccggt caagaacaaa
    62041 attcactaac tcgttgaaat tgacatattc taattgaagc tgggtattac ctgtatccat
    62101 gcgagataga tttaagaggt cattgatcat tcgaatcatt cggtctgttt catctagcgt
    62161 taccttcaaa aaatttggtg ctacttcctc gtctttccac gcaccttcgc tcaatgcttc
    62221 gatataacta cgcatacttg tcaacggtgt acgaagttca tgggaaacat tagagacgaa
    62281 ttcacgacgt tcccgttctg ttttttcttg ttctgtcaca tcatgcaaga cagctactaa
    62341 tccgctgata aaacctgatt cgcgacggat catagaaaag tctactcgca agatcatctg
    62401 atcactttcc aatgtactgg ttgaacggtc aagtaccatt tcgtttggat cttctaataa
    62461 tttccgcaac gtatattctt cttcgatatc taaaagtgtc aaaatcgatt gtccgattgc
    62521 ttcctcgttt ttgatatcta gcaaggaaac agccatatca ttgatcgtaa tgactttacc
    62581 cctgcgatct gtcgcaatga cgccgtctgt catatgggat agcacgctat ccaaacgatt
    62641 gcgttcagct tccattgtat cttgagcttc ttcaatacgt tcagctaact gattgaaggt
    62701 atcagccagc tgtccaagtt catcttgtcc atgcactttg actttccgac tgtagtctcc
    62761 tctagcgatt cgaatggctt gttctctcat ttcaccgatc ggctgtgtaa tcgaacgagc
    62821 aactaaaaca gatacgatca ttgaaataaa tgccgcaatt agtgaggcag taaagaaaat
    62881 gactgctgtg ttattgattt ccgaatattt ttgttcgata ttacttttga cgtaaagagc
    62941 cccgataacg gcatcgccgg tcggggacag gatcggttga acattgatat tcacccgttt
    63001 atcgtcgtta tctaaagcaa cataacgttt agtcgtgaaa tcattgatat caacataatc
    63061 atttttcttt cccacagcgc tttgttcatt gacatcggtt gttcctcgga tgatcccttt
    63121 ttcgtcaacg acccgtattt cgatgatatc gctagtcgca ctgttttcaa taaatctttt
    63181 gatattcgca tttacctctt catcatctgc attatctttt cgattcaatt ccgggctgat
    63241 tgttgttgct aatgattcca ccgtttgatt catatcttta ataaatgtat tgatcgtgga
    63301 acgttctaaa ccacgtataa agtaggcacc aataatttcg atcgagatca ataaaatcag
    63361 aataaaggta atagcgatct taaaattgac tgatcgaaaa aagcggactt ttcttttcat
    63421 ccaattactc ctgttctggg ttacgcaaat agtaaccgac accacgacgt gtcactaaat
    63481 aatttggatg gcttggatta tcttcgatct tttcacgcaa acgacgtaca gttacatcca
    63541 ctgttcgtac atctccaaaa taatcataac cccaaaccgt ttgtaataaa tgttctcttg
    63601 tcatcacttg tccaaggtgc ttcgccaagt aaaagagtag ttcgaattca cgatgtgtta
    63661 actcgattgt ttctcctcgt ttcgtcacca tgtaagcttc tggatggatc gtcaaatcac
    63721 caatcgtcaa ttccgcaggt gctgcctctt ctggttcctt cgcagccgtg gcaccgcgac
    63781 gaagatttgc tttgacacgt gcaactaatt ctcggttgga aaatggcttg gtcacgtaat
    63841 catcggcgcc taactctaag cctaggactt tatcaatttc agaatctttg gctgttacca
    63901 taatgatcgg catatcataa gttttgcgta cctcacgagc aacttctaag ccgtccattt
    63961 taggtagcat caaatcaagt aaaatcaaat caggttctac ctcagcaact ttttccaagg
    64021 cttcttcccc atcatatgca gtataaacat catagccttc tttggctaaa ttgaatttga
    64081 cgatatccga aatcggcttc tcatcatcga caactaaaac ttttttcata aagagccacc
    64141 tcttatatat tattgtcata ccttctcatt ataccgtatt tctgcttttg tttcatcttt
    64201 gcctataaaa caaaagagaa aattaaaatg aaagtgcaaa atactttatt attttttgtt
    64261 ctaacttcaa taaaaaagga aaacactgat tttcacctca gtgtttcctt tttttgattt
    64321 atccttctaa aacttgtaca ccgacaccca ataatcccgg accagtgtgg actactaatg
    64381 ccggagaaat ctggccaaag tagatttggt ttgcttgtgg gaactctctt ttaagtctcg
    64441 cttccatttc aattgcttct tctttcgcat ccccatgggc aacagctaag ttgaagacct
    64501 ttgcattacc gattttttct ttgacgtaag caaccgtttt atccaaactt tttttacgtc
    64561 ccctagcttt tcctactgtg taataaatcc cttcttcatt acatgagatg attgggttaa
    64621 gctttaaagc ggttcctaca atagaagcaa ctaaaccgat ccgtccacct ttttgaagat
    64681 attccaatgt cgcaacatta aagaaaactt ttgttctttc gactaactca ttcagattac
    64741 tgatcagttg atcccattct actccttctt ccagccattt tgctgcttgg atcgcagaaa
    64801 aacctgcacc aataccaata tttttcgtat ctagtacata tgcttccaat tcttcttgct
    64861 gttgtgcaat taaacgaacc acattatatg tcccgctcaa tccactggaa attgttaccg
    64921 caagtacctt ttcataaccg tcttttttga tttgctcaaa gatttctgtg atcgactcac
    64981 catccggcaa agatgtacca gggatttcct ctggcaaacg ttgatagatc tcttcagagg
    65041 taatgtcaat tttatctgta tatacctgat ctttataaat aattttcaat gggatcatat
    65101 acatctcgta tttttttatc aattcttctg gcacatccgt acctgaatct actaaaattg
    65161 cgattttatt gtttttcatt tgttttctcc tcttttaaat attcggtttc tagaacaatc
    65221 gttttttccg caagaagttt cattgcaaac gataaagttg ccgctcttac agcaatatat
    65281 tctacagggg ttgcctggtc gaatttttgc ggatgagatt taccttgtgc taattggctg
    65341 accatccaca cagctgcttc ttgttcatcg caaaatagat tataggcttc acgaattcct
    65401 actgtttttc cttggaacaa tatcccttct ttgatttcag gaatagtcaa tacttgtttc
    65461 aacgtagtga ttgcaagtaa aaatgctacg tgttctttgt tatatctttt tttaactggt
    65521 gctggtatca aacctagttt aacgtaattg tttaccatag aagaggtaag caatggatgc
    65581 ttttctcctt gtatcacagg tgaaagataa cgatcaacta acgttctgac ttggtccata
    65641 taaagttcta agtcagggag ttcttcccag tgaggcagac ggaatgtttc tagttccttc
    65701 ccccactcca ttaattgctg acgcattttt tccatcataa ccgctccttt tttcatttca
    65761 taaacatgat atcagactta tgataaacat tcaatctagt tgctttaact aggtgatttg
    65821 attagcggga ttccatgaaa acaaaaaaca agaaggctaa gccttcttgt cttttatcgt
    65881 taagaacagg agacccgccg gggagacggc gggaaaggag taaaaaatga aaaaagtgtt
    65941 agggtttgtt ctcttgcaag aactttgttt gttggtatgc ttatataata gcgagcaaat
    66001 gtgaataatc tatgaatgat ttcctataag gaagtaaaga tttttctgat tttctttaaa
    66061 tgtagtcttg cattttaaaa ttcttagtgc tactattatt aagtactttt tgtacgggcc
    66121 gttagctcag ttggtagagc agctgactct taatcagcgg gtcgcgggtt cgagcccctc
    66181 acggcccatt gggtgccaaa cccacggaac aggtaaatgt tggtgcgaaa gcatttcgaa
    66241 aacatttacg ctgttctttt ttta
//
LOCUS       DACDIH010000006        65415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDIH010000006 DACDIH010000000
VERSION     DACDIH010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 65415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.006"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            477..1010
                     /locus_tag="IV281_000392"
     CDS             477..1010
                     /locus_tag="IV281_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP8779434.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1500..2024)
                     /locus_tag="IV281_000393"
     CDS             complement(1500..2024)
                     /locus_tag="IV281_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8779435.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(2017..2490)
                     /gene="tsaE"
                     /locus_tag="IV281_000394"
     CDS             complement(2017..2490)
                     /gene="tsaE"
                     /locus_tag="IV281_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP8779436.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(2613..3593)
                     /gene="pta"
                     /locus_tag="IV281_000395"
     CDS             complement(2613..3593)
                     /gene="pta"
                     /locus_tag="IV281_000395"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP8779437.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(3746..4426)
                     /locus_tag="IV281_000396"
     CDS             complement(3746..4426)
                     /locus_tag="IV281_000396"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8779438.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4552..5421
                     /locus_tag="IV281_000397"
     CDS             4552..5421
                     /locus_tag="IV281_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8779439.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            5447..5962
                     /locus_tag="IV281_000398"
     CDS             5447..5962
                     /locus_tag="IV281_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779440.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(6009..6572)
                     /locus_tag="IV281_000399"
     CDS             complement(6009..6572)
                     /locus_tag="IV281_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8779441.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(6627..6806)
                     /locus_tag="IV281_000400"
     CDS             complement(6627..6806)
                     /locus_tag="IV281_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779442.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(6883..7392)
                     /locus_tag="IV281_000401"
     CDS             complement(6883..7392)
                     /locus_tag="IV281_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779443.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(7488..7868)
                     /locus_tag="IV281_000402"
     CDS             complement(7488..7868)
                     /locus_tag="IV281_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8779444.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            8198..8947
                     /locus_tag="IV281_000403"
     CDS             8198..8947
                     /locus_tag="IV281_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP8779445.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(9131..10408)
                     /locus_tag="IV281_000404"
     CDS             complement(9131..10408)
                     /locus_tag="IV281_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779446.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10487..11131)
                     /gene="pcp"
                     /locus_tag="IV281_000405"
     CDS             complement(10487..11131)
                     /gene="pcp"
                     /locus_tag="IV281_000405"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP8779447.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(11330..12274)
                     /locus_tag="IV281_000406"
     CDS             complement(11330..12274)
                     /locus_tag="IV281_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP8779448.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(12276..12962)
                     /locus_tag="IV281_000407"
     CDS             complement(12276..12962)
                     /locus_tag="IV281_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP8779449.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            13435..13755
                     /locus_tag="IV281_000408"
     CDS             13435..13755
                     /locus_tag="IV281_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP8779450.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            13916..14821
                     /locus_tag="IV281_000409"
     CDS             13916..14821
                     /locus_tag="IV281_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8779451.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            14818..15591
                     /locus_tag="IV281_000410"
     CDS             14818..15591
                     /locus_tag="IV281_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8779452.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
     gene            complement(15768..17192)
                     /locus_tag="IV281_000411"
     CDS             complement(15768..17192)
                     /locus_tag="IV281_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8779453.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            17403..17849
                     /gene="nrdI"
                     /locus_tag="IV281_000412"
     CDS             17403..17849
                     /gene="nrdI"
                     /locus_tag="IV281_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP8779454.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            complement(17919..19901)
                     /locus_tag="IV281_000413"
     CDS             complement(17919..19901)
                     /locus_tag="IV281_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8779455.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(19915..20811)
                     /gene="murQ"
                     /locus_tag="IV281_000414"
     CDS             complement(19915..20811)
                     /gene="murQ"
                     /locus_tag="IV281_000414"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP8779456.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            complement(20814..21875)
                     /locus_tag="IV281_000415"
     CDS             complement(20814..21875)
                     /locus_tag="IV281_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8779457.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(22087..22614)
                     /locus_tag="IV281_000416"
     CDS             complement(22087..22614)
                     /locus_tag="IV281_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8779458.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            complement(22681..24183)
                     /gene="lsa"
                     /locus_tag="IV281_000417"
     CDS             complement(22681..24183)
                     /gene="lsa"
                     /locus_tag="IV281_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8779459.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            complement(24441..25562)
                     /locus_tag="IV281_000418"
     CDS             complement(24441..25562)
                     /locus_tag="IV281_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8779460.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            25688..26629
                     /locus_tag="IV281_000419"
     CDS             25688..26629
                     /locus_tag="IV281_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8779461.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(26772..27668)
                     /locus_tag="IV281_000420"
     CDS             complement(26772..27668)
                     /locus_tag="IV281_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP8779462.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            27950..29290
                     /locus_tag="IV281_000421"
     CDS             27950..29290
                     /locus_tag="IV281_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8779463.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            29485..29928
                     /locus_tag="IV281_000422"
     CDS             29485..29928
                     /locus_tag="IV281_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP8779464.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(30190..31608)
                     /gene="nhaC"
                     /locus_tag="IV281_000423"
     CDS             complement(30190..31608)
                     /gene="nhaC"
                     /locus_tag="IV281_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8779465.1"
                     /translation="MEKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKKYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDILEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(32012..33235)
                     /locus_tag="IV281_000424"
     CDS             complement(32012..33235)
                     /locus_tag="IV281_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP8779466.1"
                     /translation="MTTTLSTNQLKDLIQYRLPEYQALALAIHDHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTGFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDEIGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDAALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAALSTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSEKGLASIAIAAELLANTALDLLREPKLLEKIKQE
                     HQEIVARKKQEGATF"
     gene            complement(33267..34106)
                     /locus_tag="IV281_000425"
     CDS             complement(33267..34106)
                     /locus_tag="IV281_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP8779467.1"
                     /translation="MKKIIRFAVITFVAIIWLAGCSQGKAATTEKKETTVKLGIIGED
                     TDVWDDVKDRLKKENIHLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNEEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENKKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDEHARPYVNIIVARKEDEDNKTYQKIVDAYQTEETAKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(34123..34803)
                     /locus_tag="IV281_000426"
     CDS             complement(34123..34803)
                     /locus_tag="IV281_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8779468.1"
                     /translation="MTEFFKTYLPNVYLIPDEFIEATKQTLYMSFWTAFIGGIIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILVLVFLSQFISN
                     QLIKKVTH"
     gene            complement(34805..35878)
                     /locus_tag="IV281_000427"
     CDS             complement(34805..35878)
                     /locus_tag="IV281_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8779469.1"
                     /translation="MIELRDISVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEAGK
                     IIEKGSSIQIFSHPEEELTKDFIRTATHLDQALETISAHAAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKVTILQKNELDNRIKVIEGGN"
     gene            complement(36528..37199)
                     /locus_tag="IV281_000428"
     CDS             complement(36528..37199)
                     /locus_tag="IV281_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8779470.1"
                     /translation="MKGAIFDLDGLLVDTERTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVQAAGSPEKVQEVRAKREEYFYQQLTSGAIQLKPYAKEILTDLKERHM
                     KLGIATTTVTKRATDILDHFQLADYFDTFTFGDEVSENKPSPVPYLTALERTGLEALS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYSVEEKEKLNLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(37287..39965)
                     /gene="mgtA"
                     /locus_tag="IV281_000429"
     CDS             complement(37287..39965)
                     /gene="mgtA"
                     /locus_tag="IV281_000429"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP8779471.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASPNGLS
                     EEQIERSRETYGENAISYGKKTPFIIEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGEYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNDTLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEAAETLDTDTINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVAPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELITGEKISEMNDHELAQVAEEHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKIPFIQSNATFAMLVITTLGIIVGSVLPFTGFGAELGLM
                     PLPGNYWLWFFITVVAYLALVTAVKKIYIRRFQELL"
     gene            40625..41062
                     /gene="ndk"
                     /locus_tag="IV281_000430"
     CDS             40625..41062
                     /gene="ndk"
                     /locus_tag="IV281_000430"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP8779472.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFATMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKGADAVPGTIR
                     GDYALPGTENIIHASDSRDAATKEIARFFPPFDTNKPSEKAAK"
     gene            complement(41084..41950)
                     /locus_tag="IV281_000431"
     CDS             complement(41084..41950)
                     /locus_tag="IV281_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8779473.1"
                     /translation="MYDGELIKKLRVNKKLTQTQLAEGICSKTSLVGIETNSVKKVSF
                     GTLKSFLERMNMTLAEYEWIRNQLDEPKKLKKKRRMLDRIQEDSFDPYKEIANNRRHY
                     KKTEDLYYLVLNLHMSKKTEVSETEFEFLRYECRIIEEYLMQVPEYGQFEVSILADFP
                     WIFSDRFIENNYLKIKKRMRRIAQLNNHEQYLFTFLTNLTLFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKSENHQEFFAVIEYTLGKNQRNSL
                     EKKLLAIAKPSS"
     gene            complement(42184..44262)
                     /locus_tag="IV281_000432"
     CDS             complement(42184..44262)
                     /locus_tag="IV281_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8779474.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPATLGQTEGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTIL
                     YPVTGTNAEQ"
     gene            44755..45972
                     /locus_tag="IV281_000433"
     CDS             44755..45972
                     /locus_tag="IV281_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8779475.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETREKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEASPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(46431..47054)
                     /locus_tag="IV281_000434"
     CDS             complement(46431..47054)
                     /locus_tag="IV281_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779476.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWEEYKLTANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLVVLLLTHDGDKVARLKNGKVVTAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     ASSQKNESTELSPSDSGSGQTITGNEENQASIITNESSEGQYQTYGQ"
     gene            complement(47210..48607)
                     /locus_tag="IV281_000435"
     CDS             complement(47210..48607)
                     /locus_tag="IV281_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8779477.1"
                     /translation="MKRYQFPKNFWWGSAASGPQTEGRISGDGKGENIFDYWYKNEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HHYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFQLFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKAYHEMDQSGVI
                     GIILNLTPSYPRDESNPEDVKAARIADAFFNRSFLDPAVKGEFPSELIERIKELDLMP
                     EIHPDDLTIIKENTVDLLGINYYQPRRIKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEKGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDRNGFEA"
     gene            complement(48629..49600)
                     /locus_tag="IV281_000436"
     CDS             complement(48629..49600)
                     /locus_tag="IV281_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8779478.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMEKISEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISNRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHAIDGLIVFLSEEEWSGFEMLTKMSIPVVALNCPGGPAGIDTIVVNDR
                     ESVAKVIAHHGGQGHKTIGFIAAAQTDYVARERLAGAKEAVQKDPSIHLEIFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            complement(49607..51907)
                     /locus_tag="IV281_000437"
     CDS             complement(49607..51907)
                     /locus_tag="IV281_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 3 glycosyl hydrolase"
                     /protein_id="HAP8779479.1"
                     /translation="MYDYPYQNPKLTIRQRVEDLLSRMNIAEKVGQVNQHLYGWECYE
                     KNGDKIELTEKLKEHVKWGGGLGALYGLFRSDPWSKTDYKNGIPAKESWKVANLVQEY
                     VLQHSRWKIPVLLVEECPHGHQGLDGISYPTNIGRGNTFNVALLEQSSRLMAKELAAK
                     GVHLALVSTLDLAKDPRWGRTEECFGEDPILSARFSEAVVRGFQGEIISEKVSFLDQT
                     VEEINKKKDQIGVVLKHCIAQGEALGGHNSGTVTIGRREFSDIYGPLLKSTRNAVGVM
                     AAYNDIDGIPCHINRALFEQTLRKEIGYQGIVMADGVALDRLSDVFTDKKTAAAYALA
                     AGIDLSLWDETYTKIAEAIDNQVVDEKLLDQAVRRVLSVKFLLGLFEQPFANDPKEYW
                     DHLMEESEHLNLETAKESITLLKNDGILPLDQQVKNIAVIGPNAHDVYHLLGDYSAPQ
                     TEDRLKKKIVQEIKTEFPQSKVSYAQGCEVRNQEDQEEKLLEAVTLAQNSDVIIAVLG
                     GSSARNFDMEFLRNGAVSSKGINMDSGENVDVASLSLGGYQEQLIEQLYQLGKPIITI
                     LVQGRPYDLMKIESFTNAIATAWFPGQEGGKAIAQMISGRNNPSGRLSLSYPRNSRQL
                     PVYYYQRAASKQENYYDLSGSPFYPFGHGLSYTTFEYSQMEVKTDIDKVIISIKVKNT
                     GSVSGKTSTLVYVRLIDGVVIQRTKLLKAFRKDELRSQEERLLVFELTSEDFSYMDID
                     DKRHYAKKAVIMVEQQEKEIKCNWQE"
     gene            complement(51909..53234)
                     /locus_tag="IV281_000438"
     CDS             complement(51909..53234)
                     /locus_tag="IV281_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8779480.1"
                     /translation="MKVVDRLSEKMLPIVSKMSSQRHLLAIRDSFIVTMPLVMAASVM
                     VLLNALIFSNATVQRILDLSPLAELASIVNNGTMSILAIIVCYNIGWNLATHYIQTGV
                     IDDPSFSPVHAGILSTAVMFILMSLSSTVTLMDGNTAEATGVFLQSLTSSSGLATAMI
                     AALVSTELFIKLAKMKSFKIRMPEGVPPAVAASFNSLVPEIVVILFFSVVVFTLNHTT
                     GLNVPQLIELAIQTPLKAFVLSVPGILFLQFFSDFLWVFGMHGSSILAPIRQAPLLQS
                     IQENMDAFSVGKEIPNIITEPFTNAFGLIGGGGCILPLVIAILWASKRQEQRSIAKFG
                     LTTCLFNITEPIMFGLPVVMNPIYMIPCAIIPSINLVIAYAATSLGIISKTVAAAPWI
                     TPPVIQSFIATGGDIRAAVLTVILIILDVFLFLPFVLAANKAKLAEGGY"
     gene            53465..53890
                     /locus_tag="IV281_000439"
     CDS             53465..53890
                     /locus_tag="IV281_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779481.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGADSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATVTFTKIGNYRLKDKDGNIYSL"
     gene            complement(54388..55089)
                     /locus_tag="IV281_000440"
     CDS             complement(54388..55089)
                     /locus_tag="IV281_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8779482.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDTFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(55105..55545)
                     /locus_tag="IV281_000441"
     CDS             complement(55105..55545)
                     /locus_tag="IV281_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HAP8779483.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTITSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVLAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFEKLPVETYVGEYL"
     gene            complement(55545..56120)
                     /locus_tag="IV281_000442"
     CDS             complement(55545..56120)
                     /locus_tag="IV281_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP8779484.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(56531..57037)
                     /locus_tag="IV281_000443"
     CDS             complement(56531..57037)
                     /locus_tag="IV281_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HAP8779485.1"
                     /translation="MDYKKRVQQMFLDSGVVLKETELERIEYTDFGLGAIETEGLNLF
                     VYENNERYCGKELVLLPYQTCPEHRHPPRDENEGKKETFRCRKGLVYLYVEGKETKKL
                     SCIIPKGQERYYTAKKEIILHAGDQYTIEQNIKHWFQAGATGAVITEFSSSSDDPSDS
                     FTNPAIYR"
     gene            complement(57048..57527)
                     /locus_tag="IV281_000444"
                     /pseudo
     CDS             complement(57048..57527)
                     /locus_tag="IV281_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(57699..58400)
                     /locus_tag="IV281_000445"
     CDS             complement(57699..58400)
                     /locus_tag="IV281_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8779486.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQEILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(58508..59950)
                     /locus_tag="IV281_000446"
     CDS             complement(58508..59950)
                     /locus_tag="IV281_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8779487.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            complement(59964..61829)
                     /locus_tag="IV281_000447"
     CDS             complement(59964..61829)
                     /locus_tag="IV281_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8779488.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(61950..62801)
                     /locus_tag="IV281_000448"
     CDS             complement(61950..62801)
                     /locus_tag="IV281_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8779489.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(63111..63824)
                     /locus_tag="IV281_000449"
     CDS             complement(63111..63824)
                     /locus_tag="IV281_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8779490.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(63892..64218)
                     /locus_tag="IV281_000450"
     CDS             complement(63892..64218)
                     /locus_tag="IV281_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8779491.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(64341..64568)
                     /locus_tag="IV281_000451"
     CDS             complement(64341..64568)
                     /locus_tag="IV281_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8779492.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(64890..>65415)
                     /locus_tag="IV281_000452"
     CDS             complement(64890..>65415)
                     /locus_tag="IV281_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8779493.1"
                     /translation="LEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRGVFLKFLEKAES
                     EKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITINLLEEKEGSRF
                     SIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELKIEFSKQNHYFF
                     VEMYLASRKSKSAY"
BASE COUNT        18888 a        13863 c        11337 g        21327 t
ORIGIN      
        1 actgcatcaa gtaataagaa cagccaaaca aaaatagttc ctcatatttt tcgtttggtt
       61 ggacttatta ccgcagatgc aagcttttga acaccgttta ttgagaagtt gtgaaatcag
      121 cgtcctgacc tgagtattag agaaacaaat ggcaaaatag ttcctcatat tttgctattt
      181 tttgagctaa taccgagggt cagctgattg aacaccgttt agtggagaag ttgtgaaaaa
      241 gtcgcctact ttcgagtcgt aagaaatttt tttgaaaatg gttcctcaca actttgcata
      301 aatttgactt aataccgaga agtcatgatc cccatctaac tccaaatcac aaggaatgag
      361 ttcaaaaact agcttttctc aacaaatatt acataaaaaa ttctatgttt tttgttaaat
      421 ttatttgctt ttttcaaaag tatgttacag taatttccca aggaggtttt acagaaatga
      481 attttgttgc catggatttt gaaactgcca atcatcagcc ttatagtgct tgttctcttg
      541 cgctagtcat ggtaaaaaat agccagatcg ttgatgaatt ctatacattg atacagcctg
      601 agactccttt tttctggcga aatgtccaaa ttcacggaat ccatcaagaa gacgtacgca
      661 atgcgccgaa attcccagaa gtctggcaaa ctatccaacc atattttcaa aaaaatcgtt
      721 tagtcgttgc acataatgct gcttttgata caaaggtctt agcgggatgc ttagattatt
      781 atcatcttga acagcctaat tatctttctt tatgtacagt gaaaagtagc cgtcgcctgt
      841 ttcctgaatt tccaaatcat cgattgaaca ctgtgtgcga aaatttgaac atccaattag
      901 atcatcacca tgatgcattg gaagatagtc gtgcatgtgc ggaaatcctg ctttatcaag
      961 aaaaaaaatt cgggacagat ccattgaaaa aacttgtgac tataaagtaa aagaatactt
     1021 atgaaacaca caaagaggtc gtgagcctgc cgtttcactc cgagtcacaa ggagcaattt
     1081 caaaaaatag cttctcatgt ttattaaata ttgtgactgg tgaccgagga gctggcagga
     1141 gaacaccgtt tattggagag gttgtgaaaa agctgttctg catcaagtaa taagaacagc
     1201 caaacaaaaa tagcttctca tatttttcat ttggttggac ttattaccgc agatgcaagc
     1261 ttttggacac cgtttaccaa gaggttgtga aatcagcgtt ttgacctgag tattagagaa
     1321 acaaacggca aaatagttcc tcatattttg ccgttttttg agctaatacc gaaggtcagc
     1381 tgattgaaca ccgtttattc aaaaaaaatg gactgcgaca cgacttttgt cacagtccct
     1441 tcgtgtgttt ttctgccttc tcaaaaggat gacagtttct cactcttttc ctatttcttc
     1501 tattcaatca aataactcat tttatataca tctaaaaatt cacctagatc tgttcttgcc
     1561 cctctaggca tgaccgcttc gatttggaag cccatttttc ggtaaaggtg tacggctctt
     1621 tcattacgaa cttgaacgtc aagctccaat cgaaaaagta cacccatttc cttcgcccaa
     1681 ctgatgattt cttctagcat aagagtgcct agtcccattc cccaatattc ctgcagaata
     1741 ctgataccga cttctcccac gtgagacaaa cgaaattgac tgtctgcctt gacagatgct
     1801 gttccaataa tcgtcccttc atagattgca agcagcaata aattattatt gctttcgtat
     1861 atataatcca actcttcttc taaggtttct ggtggtaaca ggagttctgc ttcatccaaa
     1921 actaaaaatt ctgtctcctt attaacttgt cccatcatca aaagaatgtc ttttgcatct
     1981 cctggcactg cttcccgcaa gatgatccca acttcttcac tcatgatttt cagcccattt
     2041 ttctagaatt cgctgtttga aggtttcact ttgctccccg tatgagatta gtttcacata
     2101 ccgcttttct aaatctgtat cacttttttc taaaatcagt tccaaataat cctccggcag
     2161 tgcttcttct aatagatttc cccattcaac aacggaaagc ccatcacctt caaaatattc
     2221 atctaaccct aaatctgctc cgctagctgc tacccgataa atatccatat gatacaacgg
     2281 aatccgacct tgactatatt ctctaataat cgtgtatgtc ggacttttga tcatttgcgt
     2341 tatccctaat ccttgggcaa ttcctttcgt caacgttgtt ttacccgcac ctagatcacc
     2401 agtcaatatc agattgtcac ctggcattgc ggaatgacca attgcctctg cgaattgttc
     2461 tgtcattgtt aatcctgata gttcgatcat ttttcatctt ctcccgctct tctttctttt
     2521 tgccagtata tcgcgtaatg aagttttttt aaagaaaaaa tgaactagtc aagaacagca
     2581 gctgctgttt ttggctagtt cgcgtgtatt tgttaatcaa tcaatgtttg tgctgctgtg
     2641 ataatcgata atttatatac atcttcttca tttgctccac gagacaaatc agaaaccggt
     2701 ttgttcaagc cttgtagaat tggtccgatt gcttcaaagt taccgaaacg ctgagcaatt
     2761 ttatagccga tatttcctga ctgcaattct ggaaatacaa agactgttgc ttgaccagct
     2821 acttctgaat ctggcgcttt cagttgtgca actgaaggaa cataagaagc atcaaattgc
     2881 aattcgccgt cgatcatgta ttgtggcgct agttttttag cgattttagt tgcttcaact
     2941 actttgtcta cttctggcga tttcgctgag ccttttgtcg agaagctcat cattgctact
     3001 tttggttcga tatcgaacaa ttctgctgtt tttgcagaat ctacggcgat ttcagccaat
     3061 tcttgcgcag ttggattcac gttgatcgca caatccgaaa agacatattt ttcttgatct
     3121 cttccacgta ccatgatcat cgctccgctt gtccgactga tgccaggctt tgttttgatg
     3181 atctgaagtg ctgggcgaac agtgtcgcca gtcgaatgga tcgctccaga gaccattccg
     3241 tctgcaatcc ccatatacgt aagcatcgta ccaaagtaat tcacatcttt taaaatcttt
     3301 tctgcttgtt ctttggatgc ctttccattt ctacgttcaa caaaagtatc caccatttca
     3361 tcccacttat cgtaattatc agggctgatg atcgtaaagc tagatgtttt gattcctctg
     3421 gcatgcgctg catttcttat ttcttcttcg ttccctacta aaatgggttc cacaagttct
     3481 tctgctttca accgggcagc tgcgcctaaa atacgagcat cagtagcttc cgggaaaacg
     3541 attttgatcc cacggcgaac aattttaaat cttaaactat caaacaattc cacaagatga
     3601 cctccagacg tttttatatg tattatcata cactattcca gaaaaaaaga acagtttatt
     3661 ttatctgtat cagtcaaaaa tgctggaaaa attaagtttt tcacatacaa agcgaactgc
     3721 actataacag gaaagcgctg ttttattaca ctgtttcagg cagttgccaa tcaatcggtt
     3781 cttctcctaa cgcaaggagc gcatcattcg ttttcgagaa gggtttggaa ccaaaaaagc
     3841 cacgacttgc agataacgga ctgggatgtg gagatgttat gatgatatgg cgactcttat
     3901 cgatcatttt gattttttct tgtgccggtt ttccccacaa gataaacacg attggttttt
     3961 cccgttcatt taatttttca atgatcgtgt ctgttaatct ttcccaacct ttcccgcgat
     4021 gagaatacgc ttgcccttcc cgaacggtaa gaacggtgtt gagtaaaagg actccctgtt
     4081 tagcccatga cactagattt ccatgttgaa caggtgcgat tccaagatca tcataaagct
     4141 ctttatagat atttcttaac gatggcggga tctttacgcc aggttgcaca gaaaaagata
     4201 agccatgagc ttgattttct ccatgataag gatcttgtcc taaaatcacc actttaactt
     4261 cttcataagg agtcaattcc aatgcttcat agatatgata catatctgga tggatctttt
     4321 gggacgcata ttcttttttt aaaaattctc ttagatgcag ataataatct tttgaaaatt
     4381 ctgaggacaa aacttcttgc caactgttat gaatgatttc tttcaaaaac tccacctcct
     4441 aataaaaaga ataactcaca ttttcttcaa tcacaaggga caatcttcac cctcagcact
     4501 tgattaatat ggtaaactag gaacaagaag cagaaaggaa cgataatcat tatgatcaaa
     4561 ttaattgcat cagatatgga cggcacatta ttagatgccc atatgagtgt ttctcctgaa
     4621 aatgccgagg cgatacgttt tgcaaatgat gccggtgttg agttcatggt cgctactggt
     4681 cgaaactatc aagaagctcg cgcagcttta gatgaggtag gaatcgattg tgcgatgatc
     4741 actttgaacg gggcacaggt atttgataaa gaaggaaatt cattgtttac cgtgtcgatt
     4801 ccaaataatc aagcaatctc tgttttagat attctagatg ctaacggtat ttactatgaa
     4861 gtagcaacca acgatggtct ttattctgaa aatcaagcga aacgaattga aagttttgct
     4921 tccatggtgg ctacccattt acctcacctt acgtataaaa tggcgattgc catggcctca
     4981 gctaagctgg agttattgca catcacatac gtggacagta ttcgtgctat tttggaagaa
     5041 aagaatctgg aagttttaaa aattatttgt tttcatacag aagggccagc tgtattaggt
     5101 ccagttggaa agcagatttc tcttttaggc gatctagccg ttacttcctc tggtcaaaac
     5161 aatatcgaag tgaaccataa aaacgctcaa aaaggaattg ctgttgccca tgtcgctcac
     5221 gagcgtggga tttctttaga agaagtcatg acgattggcg ataacttcaa tgatgtcagc
     5281 atgctgcaag tagcaggagt cagtttcgca atgggaaatg cagaaatcga agtaaaagat
     5341 tatgccaaat atacaacgga cacaaacctt gaatctggcg taggaaaagc catattgcgc
     5401 gcgattgcag aagacttgta gttttatcta gaaaagaggg atatagatgt cagaattttt
     5461 cattcaggaa aagcagcttg gacgtgcaac tcggacaatt gtcaaaaacg aaaaaggaca
     5521 ttccctgttt ttgatggttg gtcgctgggg aacaaagggc gatgcattgt ctctttatgc
     5581 catgaatggc gatctcgttg ctcacatcaa gcagataagc ctgacattcg gtacgcgttt
     5641 tgagctgtat aaagagttcg aaaaggtggg aaccatgcag aaaatattca attggcccgg
     5701 tgacttttat tatattcgta gacttcactg gacagtccaa ggtgatatct ataatcatca
     5761 atatcaaatc catcatttca atcagcaaat catgaaaatg gataaagcaa cacttttttc
     5821 aggagattac tatgtattgg atgtctcaaa tgatgaagat gctccagttt gtatttgtat
     5881 tgctgcggtt ttagattact ggctctaccg taaagacaaa agtcgcgata agcctgtttc
     5941 ttaccgactt aacttaagtt aaaaagatgt acctgaaatg cggaaatacg catttcaggt
     6001 acatcttttt agatattctt ttgcatatga tcataaagat gaccactgat catccgctga
     6061 ccgacaactt tgaagtcatg gcgttcgtat aattttttag cctttggatt tttttcatcc
     6121 acacttagac cgatgacgga gcgattagct ttcttcgcca atttaggtaa tgcttctaat
     6181 aatcgcgaac cgatgccttg tcctctaaaa tcttctgata cactgataga gtccaaatac
     6241 cattcgttgg gaaacgtttc tttatcaatg aacaaacgtg tagacgcatc tagctgatat
     6301 ttctctaaga taggtaccag tggctgatcg atgattgatt cttcttcatc tgtataaccg
     6361 taagcaactc ctgcaacttt accgtctatt tcttctacga tacctcgtga ataactgtaa
     6421 cgataatttg gatcagtgat tgcttcttct aaaatctcta aagtcttttg ttcccctgtc
     6481 tgcttcaaaa atggcagttc catatctttt agaatcacta agatcaatgg ggcgatttct
     6541 ggagcatctt cttttctcgc aaaacgaatc atagtttcct ctccttttct ccattctaac
     6601 atgcagaaaa agaaatcgca aaatcgttat tttaaaaaat cttcaatctt ttcagtaatg
     6661 aaataaccat gagtaacgat tcttgccgct tcagttcggc aataagggca gacgactggc
     6721 atttctttat cattatgttc gactacatta ttgacttctt cgtatgcaaa tttttttccg
     6781 caattagggc atttcatttt ttccatgtta tctcaacttc ctttcatctt tactctctta
     6841 gcttactctt atattgtaaa aaagagaagc aatatccttc ttctatgcag atatccatac
     6901 ctgacggtcc tgttctccat ctattaatcg gactttataa taaggacgtt catctttgac
     6961 tagaagtgtc cactctttca cttttgcttt tttcccgtag tagctgactg cttccttcat
     7021 cgctgcttgc ggttgtttga actcttccgt tttttctata tcaaaaagat gttctgcggc
     7081 cttttccgtg tcatttcgta cggttgtttt cccagtggat ggattgactg ctatgacttg
     7141 tccttcacaa aaaaactggt attcatatgt tgcattcgta ggattctcca aatctttttc
     7201 aactgcaaaa tgaatagcag atatgttatg ttcttttgtt tcgtttttta cgattgctgc
     7261 tgcttttttc aatgagatcc gatattcctg gtcactaata tcatccatgt gaaaagcggc
     7321 attgagcagt cccgtcatac cggtaatccc tattacgatg agggcagcaa caccaaacat
     7381 catttttttc attcttcatt ctcctgtttc ttttttttaa agaatatcaa gaaatgaacg
     7441 aatgaaaaat cgaatgaaac gaaaaaaaag tgacaatttt gttatcttta ttcgtgcagt
     7501 tctaatggca atccgtctgg atcaaagaaa aacgtcattt tttttcctgt aaaggtatct
     7561 tctctaatcg gttctgattc gatccctaag gactgcaatt ctgcaattgt ttcttcaata
     7621 tcgtctactt tgaaagcaag atgacgcaag ccacaagctt ctggatagtt tggtcgttgt
     7681 ggcgcattag caattccaaa aatctccaat tcagcacttc ctagtttcag atctaattta
     7741 tagtcatctc gttctttgcg gtaattttct ctgattactt caaatcctaa tttattaaca
     7801 taaaaatcac gtgatttctg ataatctgaa acaatgatgg ctatatgatg gatcgttgta
     7861 aggttcatat gagcactcct attcttcgtt taagctcgga tctatattaa tctccagttc
     7921 cattgtaaat gtgtcagatt gaaaaaaata gctgtttttc ctttttataa gaaaaaaggc
     7981 aaaaaaaaca gaaagtaggt cctccaagac actcttcctg ttcccaatat aataaatgaa
     8041 agtaagaaat tatattttca agaaaacaaa cagaaaatta tcataacaca aattatttat
     8101 aattgaaaga ctagtttttt ttcaagaaaa gcatacaata gattgtgtta gtaaatacag
     8161 aacacactac atttagtgtc ttaaggagtc ctcatacatg aagtcatatt ttcagttttt
     8221 agctcatcag ctaaaaggct ggccgcaaca aaattattat cttttctttt tcagtcttgg
     8281 ctgccaggtc atgactcttg tcaatagtcc catcacttgg ctgtccgtta ttacatttat
     8341 cggtaccacg cttggtgtat tgtgtatttt atccatcaat gcggcaaaat cagtcaacgg
     8401 tattttaggt attttgtctg cgatttgttt tattatcgtt gggttttcag caaagaacta
     8461 tttaagtatc ggtgaacaga ttgcttatat gatcacattg gatatccccg tattgttaag
     8521 taaagaatgg aatcataata tggcttcaaa aatccgaaaa ttcactggaa aaagctggtt
     8581 tatcgcaatc atcagtactt tccttgtcta tcttgtttca ggattcctga ttggtcgtta
     8641 tacagatgat cctcggccgt gggtagatgc cattgctttt tctattagct tgactgctgg
     8701 tatcatcagt ttcttgcgtt acaacaacca atattactgg tggttagctt cagggcttgc
     8761 tcaaatggtc ctttggtata tttcatttac gcatggatct gcaagtttgg cgatgttcgt
     8821 gaacagctct gtttacttgc tgaatgatgt ccttgcattt accgtttcac cttggtataa
     8881 ccaaaaagaa cgtgaacgta tgcaaaaaat ggaagaagaa tactacaacg cacaacaaca
     8941 cgcataaaaa aactgagcgt gggacaaaag taaagaacac ttttgtctca cgctctaaac
     9001 acgtataaac ggcggaatca gaagcaactc cttcggaaat aagctgaaat tcacaaaaat
     9061 ttgaaaagca attttcgtga atttcttctt atttcttgga gttaaacgct tctgtcccag
     9121 cctctatttt ttatagtaat tccttcagat actttcttgc ctcatcattg aaacgcagtg
     9181 attcttctaa cttctgataa tattccaacg cttgtttctg agcttcagta aaacgataat
     9241 gttttccttt ccatttgtcg attgtctctt gcacatttcc agattggata tctttcacta
     9301 ctagttgcca tttttctttt gagactgtac ggtctttctt atcagcagat tgtatagctt
     9361 ctcgttcttt acgcaatact tttaaaaaac catttctgac tatccgagga atgctgtcga
     9421 agaacccttc tgtaaaacca tagcgacaat attgcttcca aataaataga tagatttttg
     9481 gttggatgaa tcgttggttt ttcgcaaaat attccatctg agcagtgact tctggtgttc
     9541 gttgacgttt tgtctttctc aacacggaaa taaatgtttc aaaataagca ttaggatgta
     9601 ctggcacttc ttcatatacg atggattgcc aatctttacg taaaaatgcg cgatagttga
     9661 tcaagaattc ttctacatcc tttactaatt cttctgcaaa gtcgtcaaat agttcttttt
     9721 ttatctgtcg cttttttgac tgcccattga tgtgttcact tattttcttg atttcatttt
     9781 ccaatgcttg ataatccgct atgtaaatcg gcatttctac ttggcgaaat tctgcgagcc
     9841 gctgagctaa ttttatatcc ggtaaatatg gctgtttcat ccgacgattt tggtacaaga
     9901 caaaacagta tttttgccat agtccttcag gaaaatcgat attcttctgc ttaagccata
     9961 ataattcgtc taaggccaga tcaacttttg tcattccctg atggatcgaa gctgccactg
    10021 tcggagagac atgcaggtga tcggcaaagt ggttgatgcc tagttttagg atctttccgg
    10081 tttttggcga tttaacgatg tattgcactt ttaattctct accacactca caatacaatt
    10141 tttgctggct tcgttttgtt cgaaaaaacg gattgatctt gaagtcagaa aacacccaat
    10201 ctgttgcagc taaataatgt tcttccataa aacgagagct gattagatat cggcggtgct
    10261 tatcgactag ttcccgctgt tttttcgtta attttgcata gatctcccaa cgttgactgc
    10321 ttgtgtattg tttagaaatt ttgatgggat agcttttgct gatttctttt ggaaaccatt
    10381 cttcaaagac ggattgccat tcttccacgg ctgtttcttt cctttctaat tacaaaaagc
    10441 tggctactaa cataacgtta gcaaccagcc agtgtttctt gctaaattag tgagttgttc
    10501 cggcatttcc ggaaatatct tctttatttt ctactgctgc ttcaactgca taagtcaaag
    10561 aagaagcaat gtcttctaaa gacatagaag gcgtacccac tggacgatcg atcacttgat
    10621 cgggtgagaa tggcacgtgg atgaacccac ctcgaattgc cgggaattgt gtttcgatca
    10681 aatgaagcaa acgatacatg atatcattgc agacaaatgt tcctgctgta taagagatgt
    10741 acgctggtag accatgatct tgtacatttt ttgtcattgc tttgattggc aatgaggtaa
    10801 agtaagcggc tggaccgttt tcttctaaag ccgtatcgaa tggctgattt ccttcattat
    10861 cagggattct tgcttctgct agattgattg cgacacgttc gaacgagact gctgaccgcc
    10921 cacctgcttg tccgacacag atcacgacat ctggttggtg ctgttggatg gcttctttga
    10981 ctacttgact gcttttttcg aatacggtcg gtacttccac tttgatgatt tcagcaccgc
    11041 tgatttcatc tggcattttt tttactgctt cataggctgg attcactttg tccccaccaa
    11101 atggatcaaa acctgttact agtactttca tctgttgttc ctccgtccga ttgatttaac
    11161 atatggttat cttttatcat accattttcc ttgaacagcg taaataaagg aaacaatagt
    11221 ttaaataaca taacagatca aacttaatat attcttataa aaaaatagtg ttccactaca
    11281 atgttagaag aggttgcgac aattattctg tcacaacccc tctcttctat tatttaaata
    11341 gaatcatata agcaatttgg aaaactagca taaataacgc aataaacaac tggtttttga
    11401 tcactccgta tttgtctttc atctccagca tcgctacagg cacgatattg aagttagcag
    11461 ccatcggtgt cagcagggtc ccacaaaaac cgcaagtcaa tgcaaccatt cctattaacg
    11521 ctggattcgc tccatgacta aatacgaatg gtgcaccgat cccaaccgtc atcacggtga
    11581 tggccgcaaa tgcattcccc atgattaccg taaatatcac catgccaaga gcgtaaatga
    11641 tgatgccgat gttcacattt ccttctggaa cgatcgtacc tactgcattg gaaatcactg
    11701 tcccaactcc agcgcttgta aataccgcac ctagagaagc taaaagcatt ggcaacatgc
    11761 ttaacggccc tactgtgccc agcatatctg ctgcatcatc taaaaatgta cttggtcgat
    11821 tatctcgaga aaagaacatc aagattagca tggctgcaaa aacaccgacg cccacgccga
    11881 ctaaagcacc taaattggta aatagagcaa acaagatcgc aaagacaccg atactcaaag
    11941 ctggaataaa aattttcatt cctagtttgt ctgacatttt ttccatatat tcttttgatg
    12001 gaagccggct ttctcctttg ctgactttct tagcaattgc tggtatggtc atcgcaaaga
    12061 tcaagccacc gttcacgatt gccggaagcc atcttcctcc tgcaataact acccctaaga
    12121 ccgtccagaa aaaggctgac ccataacgat gaggattggt tttatctgtt aaatttctta
    12181 ctccagtgta aatcaatacg agtcccatca cgatataaag gatctccagt aatttatcgc
    12241 ctaataaaat atcaggattt gtaaaaaagt ccatcttatt gcccctttcc tggataatat
    12301 ttcttcgcta atttcttttc tttaagatag aagtagacac ttgccacaac taaagccgtg
    12361 atagccactg gaacttccac taaagcaagc tgtcccaatg ttacgtcata tcctagatct
    12421 ttcagtgttg attggactag caacgcccct gctcctccga tgaatagtac ttggccaaag
    12481 aaccagcaga cattttccat agctgaagcc atacctttta gttcttcttc atgcttagga
    12541 tggacttcta acccttttga ttcgatggtc cccaatgcca tcggtaaaat gattggacga
    12601 acaaatccag caactccacc aaaactcacg ttaaaagccg caaagatcat tcgaaagatc
    12661 ccataaatat ctacaatcat accagcagat actttcttga atcgcttgat caaggaagcc
    12721 gctgattctt tcagaccatt tctttcaagc gttcctgttg ccaacataat gatgataaaa
    12781 atcgccatcc cgcgattgtt tacaaaactt gtacctaaaa gttctaacat tccttgaatg
    12841 ccaagaccgc cagtcaaagc tgtaacaact aaagcaacca ttacgatcaa tatcgagtct
    12901 aatttaaatg caaacccgag aataacaatc aagacgccta aaaggctaag ataattttcc
    12961 atatattttc ctccataaaa tgacaatatt tttttaatta taaagcaaaa cacataaaaa
    13021 atatagttgt tttctcacga aatagacact atttttaaat atttttcgtt atttttttat
    13081 tatataattc accttactta ttgtaagata aagctttttt taatttattg cctgctaata
    13141 tataaaaata aggtctagtt actgtttttt taaatgatta ataaataaca actcctttct
    13201 atgaaattgc aaagcattca tcttcttttc tgctaacgat atatccgtca atgcttgtta
    13261 cactttacta aaaagtatga gattgccgat atctggatag gtgtgctata ctacagaaaa
    13321 agatatacga tctgccaccg ggtagtaaaa aagaggatga tcattccatt aggtctttga
    13381 agggatgatt ctcctctttt tgtgtatttt ttatattgaa ggaggaacgt atgtatggca
    13441 tggatggaat taatcatagc aggaattttg gaagtattct ggtcaacgat gatgaaatgg
    13501 agtgatggtt tttctaaaat caattattct ttttacacag tgattggaat gattgctagt
    13561 ttttactttt tgtcaaaggc aatcaaaagt ttgcctatga gtctctctta tcctatttgg
    13621 acagggattg gcgcagttgg ttcggtcatt attggtgtgg tcttgtttca tgataagctg
    13681 aatctttcca cttggttctt tgtcgggttg ctgctcatca gcatcattgg catcaaaatc
    13741 actagcggtc actagatact tagacaagca gacaaggact gatcagaaaa agcggggctt
    13801 tttatttgat attcctcttt ctatgaaaaa acaagaaaat aagactaatg acctagcgac
    13861 ttctctactc atcaggtgta tgcttgtact atctatagaa aaggagtgaa tatttttgaa
    13921 aagtctttcg gtccaagaat tatccaagaa ctttggtcac aaacaagccc tcagcgatgt
    13981 cagttttagc ttatctcctg gcaaaatcgt tggattagta ggtcctaatg gcgctggaaa
    14041 aacaacgatc atgaaagcta ttttaggact gatccattac tcagaaggaa cgatccgtat
    14101 tggtgaaaat gagatttctc cctctagtca taaaggatta gaacaagtag gagcattgat
    14161 cgaatatccg ggtatctatc cattcttaac aggttatgat catctgaagt tattctctga
    14221 aacaaacgat gtatctgcta tcgacactat cgtgaaacag ctcaagatgg aaaaatacat
    14281 tcacaaaaaa gcgaaatcct actcgctggg aatgaaacaa aaattgggaa ttgctcttgc
    14341 cttactgaac catccgtcta tcgttatttt agatgagccg atgaacggtt tggatccaca
    14401 agcaacaaga gatgtgcgaa aaatcattac cgacttggct actcagggca cctctttctt
    14461 gatctccagt cacttactca gcgagcttga aaaaatggtc gatgagctga tcctgatcaa
    14521 tcaaggaaaa atcatcaaac aatgtacaat ggctgaattg aaccaaacag cgcaagattt
    14581 catcgttatc tctacgacaa atgatcaagc tgctcgaacg atcttgacag acgctggata
    14641 tcccttggaa gatcctgata aaatcaagct tctaaaaaaa aacgaagagt tattaggaac
    14701 tgttatccag ttattgtctg cagaaaatgt ccatgtgaca gatgtccagc atctgacgaa
    14761 tgatttggaa tcttccctat tggaaattct ggattcagta aagggggaag aagaaaaatg
    14821 acgttgctta taaaacaaga attatttaaa ttgatcaaga aaaagtcaac tgctgtttta
    14881 tctgttctat tagttgcttt gctcatagga acagcacttc tagccaaaaa atacacgacg
    14941 atcattgatc cagttgaaat gactgctcag cttttcagtg ccacatcttg gattgtcttt
    15001 atcatgattg ctgctgcaag tacgatcatt tcaatggaat cacaatatgg tacattgaaa
    15061 aatctccttt atcgaaagta ttctcgagga gaaatattag taagtaaatg gatcacatta
    15121 gttatttatt ctatttattt atacctttta gctattattg tcaccgtgct gatgaaactg
    15181 atccttttcc cttcgatttc ctttacggag aagatttcta ctggtcagac actgatccaa
    15241 agcttattca cttatacttt gggtagttat atcggtttat ggctaatttt aagtttagtg
    15301 ttgatgattg cttgttttat caatagttcc ggtgcctcca tctcagcagg gatcgtcttt
    15361 tattttgctt catcaatcat atctggtatc ctcatcgcac tcattcaaaa atgggagtgg
    15421 atcaaatgga atccaatcag tatgctgaat ctgcaaaacc aaattggcaa cgaagagatt
    15481 atgaagcaat tgactcatct ttctactaat caaatgctgt ttggcaattt agcttacatt
    15541 gctttgttct tagcattggg atacctcgta ttcaagcgaa aaaatgtatg atggttcatt
    15601 aacaaagaag ttgtgacaga agtggccagt ttcaagaaat ttcggcttat ttttagaaga
    15661 agctacttct tgaacaccat ttattcaaaa aaagaagcga tgacacgaat gttgtcaccg
    15721 cttctttttt gaataaacca caggcttcac agcctctctt tatacttcta tacgtgaagc
    15781 atcccagtgg caaataaaac cattgcgatt actccgacca tcgtcacacc gataagtaaa
    15841 atcttatcta agtggatatg ttgtacaggt tcgttttgtt ctttacgtgc tttgaggtaa
    15901 aaataaatac ctggtaagaa aagaacaaga acaagcatca cttcattaaa ggctacaact
    15961 agcatggccc atacttgaaa agcacttgct aaaaacccta ctaaaaattg gacccactct
    16021 ttccgctgat aagaaagctt catttggtac agccctaaga aaagccacgt gaagaaaatc
    16081 gctgctgtac caagtgtata agcaaagtta tatgcactgt cagtgaataa taatgaaagt
    16141 aaaaagacat tcgtacataa cgtcgtcaat acgatagcat aagttggtgc cccatttttg
    16201 ttgagttttc cccatgcttt aggcaaaagt ccgtcctcag ccatattccc cattgtttca
    16261 atcggcaaga ttgtccatga gagccaaacc ccaactaaag aaatgatcaa accgccgttg
    16321 atcacgaccg ccccccattt accaaccatc atttcaaata catatcccat agcaggttgc
    16381 gctttgatcg tttgcagctg attcatcgtc attgtgccat atggaagcat ggaaaccaag
    16441 acgtaaataa tgatcaaacc gaatattcct agaatcgttg ctttcccaac atccgattgt
    16501 ttttttgctc gtgaacctac gacagaagct ccttcaatac caacgaatac ccagagcatc
    16561 accatgatgc aactcaatac ttgactagaa aacgggatcg gctgcccatt cgcattcaat
    16621 atgatatcgc tagaaaagtt attaatgaat tgttggatcg aaaaggtgct gatagctact
    16681 acaataaata aaaataatgg cactaattta caaaatgtga cgatagcatt gacaaagcta
    16741 gcattttcaa ctccacgatt cactagccat gcatatcccc ataagaagat cgtactcatc
    16801 acgatcgatg ccacattttg accaccttcg aatacgggga agaaataacc taatgcactc
    16861 atcaacaatg ttgcgaacgc cacgtttcct aaccagcatg tgagccagta accccaaccg
    16921 ctgataaaac cagcgaaacg gccaaaacct tcttctgcgt aactaaagat cccgcctttt
    16981 aaatctggac gttttttatt gatattattg atcgataagg agagcatcaa cattgctgtt
    17041 cctacgagta cccagctgaa gatagctggg ccaccagccg atccggagat gtccgtcata
    17101 ataccaaata cgccacctcc aatcgctgaa ccaatcacta aggcagcaag tggaatcaaa
    17161 ccaattcctt ttttctgttg ctctgtatcc atgtaagcat cctccaaagt aaaaccgctt
    17221 ttatcaaaaa gtataacaaa ccttttttat ttttacttaa ttaataaaaa ctttaataga
    17281 aatataattc taccgacttt tttctcatta tacaagcaaa gcgcctagtc cctttccaaa
    17341 ttacaccacc aaaaaaactt aaaaagatgc tatactaaag ggaaagaatt gggggatgat
    17401 ccatgaacat tttatatata tcgatttcag gaaatacgcg tgcttttgcc aaacatctag
    17461 cagaatatgc tgaaaaaatg catgcagaag atcctgtcaa tcctgaagtt actttaaaag
    17521 aaatccatga aaatagtgat tttacgaaag aaacagaacc attttttact ttcgttccta
    17581 cttatttaga cggcgggaat gggttagaca acggtgacac agaaatcttg accgaaacga
    17641 tgcgcgaata tctggaatac gaagacaatc ataaactttg ccttggtgta gtcggtagcg
    17701 gtaataaaaa cttcaacaat caatattgtc tgacagccaa acaatacgca caaagattcg
    17761 gctttccttt cttggcagat tatgaattac ggggaacacc atctgatgtg acacgcgttt
    17821 atcaaatttt ggcagaaaaa aataaataac acaaaagaag aagctgaaaa ataaatttcg
    17881 gcttcttctt tttatgttct ctattgtttc tattcttatt aagctttgac atccatcaca
    17941 ggtgcttttg ctttcgcttt acctgttttt tccaagacaa agttttttac acgatccata
    18001 ttggttacca agacgatcat ggaggtttct ttgtctgctg ctttgattgc ttctaagtca
    18061 gcttctgcta caagcgtatc aggtgtcact tgctgtcctt ctgttacttt cacatcgaat
    18121 ggcgtaccgc ctaagtcaac tgtgttgatg cccatgtgaa gcaagatttc taagccattt
    18181 ggtgtcgtaa tgccgatagc atgttttgtt gggaaaacag aacttattac tccagtaacc
    18241 ggggaataga tatgtccgtt ttctggttct acaaagtaac cttcacccat cattttttgt
    18301 gcaaaaactg ggtcacttgc tttatctaaa gaaacaactg ttccgtcagc aactgatgat
    18361 aagatagtca cactttcagc tgaagcttga ggtactgctt tttcaggtac ttcatttact
    18421 gtggctgttt cggtcgctag ttcttctttt tctaaacggt ctttaggaat accgaagaag
    18481 aatgtagcaa cgaagccacc agcgtaagca gccaataaac caaggatata gcctaaccat
    18541 ttaccatcag agatcaatgg aatcaaagcg gctcctgaag gtccaatagc gattgctccg
    18601 acatttccga tcattccgat cactgcccca ccgatacctc caccgataca agcagtaata
    18661 aatggacgtc ctagaggtaa tgttactccg taaatcaatg gttcgccgat tcctaagatt
    18721 ccgactggta aagccccttt gatcatttct gttaattctt tgtctttttt gcagcggatc
    18781 cataatgcca atgctgcacc cacttgtcct gctccggcca tcgcaaggat tggaagcaat
    18841 aaggtacgac cagtttcatc aatcatttgt acatggattg gcgtcaagat ctgatgtagt
    18901 ccgaacatga ccattggcaa gaataatgta cctagaacga agccggcaaa tgctcctcca
    18961 acaccaagta cccagttgat cccaccgacc atgccgtcag aaatgaaccc tgctaaaggc
    19021 atgatgaaga aaatttcgat cagaccgatg gcaatcaggg aaagagttgg cgtaacgatg
    19081 atatctaccg catcaggtac tactttatgc agttttttct caacgattga caataaccaa
    19141 actgcgaaga taacacctaa gatcccgcct tgtccagttg caagagcttc accggtaaac
    19201 aagtttttca gtggtgcttc tggattcatc cctgtcagca tcacaacagc cccgataact
    19261 ccaccaagcg ttggcgttgc accaaaaact tgtgcagcat tgatacctgt atagataact
    19321 agatagctaa acatcccgtt tttcaagatg ttcatcacat cgatatattg ttgccatgtt
    19381 cctgcgtcta gtgttccagc tgtgactaag tttgctaaaa ctgcggcaat ccctgccacg
    19441 atcccagtgc cgacaaatgc agggatcatt ggtacaaaga tattagagat gtcttttaat
    19501 accgatttga ttttagaggg tttttgtttc gatttttgtg ccgctttcat ttcttgtgcc
    19561 cgttcgttta cacggtcttt tccagatgca gaagaacttg ctgtagttgc actagcagaa
    19621 ggaagttttt ctcctaattt cacgcctgct aagtcaacca tttcctgcgc tactttgttc
    19681 actttcccag gtccgatgat gacttggagc tgttcatcct ctactacgcc aagtactcct
    19741 ggaatcgctt ttaattccga gacatttaca agatcatcat tgatgacctt catacgtaca
    19801 cgagtcatgc agtggacaat gctttcaaca ttgcccatac cgcctgctcc ttgataaatt
    19861 tgttttgcca gttcttccaa agtcaattcg ttttttgcca tggtcttttc cccctcataa
    19921 ggttcctttt acaaaaccgt ttgcttcagc taattttgct tgagctgcct ctttatcact
    19981 atttgtcaaa atcatgacga tagccagttt gacattttga tctgcttcct caaagtactt
    20041 ttcagctgtt tcgtagtcag cttctgtcgc ttgcatgatg attcgttttg agcgttcaat
    20101 tagtttttca tttgttggtt tgacgtcgac catcaagtta ttgaatactt taccgatacc
    20161 gatcatactg atagtagaaa tcatattcaa aatcagtttt tgtgctgttc ctgacttcaa
    20221 tcgagtagag cctgtcaaaa attctggtcc tgcatcgact tcgattggga aatccgcatg
    20281 ctcgctgatt tctgcttgtt tattgcaaga aatcgttcct gtaacagcac caatcgattt
    20341 tgcgtaatct aatccaccaa tcacatatgg tgtacgaccg ctagctgcga taccaataac
    20401 tatatcattt tttgttaaat tcagcttgat tagatcttct tgccctaatt cataagaatc
    20461 ttcggctcct tcgacggcta cagtcattgc ttcttctcca ccagcaatta ggcctatgac
    20521 catttccggt tctacaccga atgtcggtac acattcagca gcatctaata caccaagtct
    20581 accactcgtt ccagctccca tataaatcaa gcgaccacct tttttaaagg attcaatcgt
    20641 gttggcgatc actggctcga tacttggcag ttctttttca acagcagcgg ccactttctg
    20701 atcttctttg ttcatgagtt ggactgcttg agaaatactc atttcatcta atccaaaagt
    20761 ttcttggttt ctgcgttccg ttgttaggtt ctctaaattg attttgctca tttttatctt
    20821 ccttcttttc ttataaattg atagttcata ccagctttga tttggttgat cagcggtagt
    20881 tcttctttga tgactttggc tactacattc actttttcat ccgctggcaa atcatattta
    20941 gttaattgga tctcacccat atagcgcaaa tacttttcgt tatctaaagt aatagatcct
    21001 ttcatacgct tagctgtatt tcttactggg attggcggaa tctctcgaaa acgcgcatct
    21061 gcgcttcgta tgacatctct tgcatcatct tgccgattcg tatgttttcc taatatatat
    21121 tcgtaaaaaa cttcatctac aggttccgta tggagcaaga ctgtctgttc tttttggaaa
    21181 gaagcaaatt gctcctgaac gtgttctgac agacctgcat ctccgatata aacaatatcc
    21241 gtgttacttg ctaatagatc aagagcacct gccagaggat ggatccctcg atgttcttcc
    21301 aaagttggta atccttgata cagtggacct ctcaactctg tatctcctgg tacaaagcct
    21361 tgtacaagga atccttgagt tttgagccat tgattttttt cctgatacca ttctttgtcc
    21421 aaccctgttt caagacgtgg ataataatta tgccaagctt caatctgtga gaaatcagcc
    21481 gctgcttctt tcaactcatc gatatcatta ggcgtgatcg tgctggcatt caaactgatt
    21541 ttcaattgat gtgaccagtc ggcgatctgc tgattagaaa tatgataatc cattcttaac
    21601 cctgttacac caattgcttt caagggtgtt agatcttcta atgaaaagcc agctcgtttc
    21661 aatgcctctc ctgaaatatc gaccatcaac tctagtttat tctttttcgc ttcgcttcct
    21721 agttcgatca atcgattttg ataagccgaa acatcatctt ccggtatatg catggaagtg
    21781 aaaataccga caaagccatt ggatgccatt tggcggatat actgtttctt ctcttctgac
    21841 aaagaatcat tcataaatat tgaaaagcca aacatattat ccctcttttt ctgtatccgt
    21901 cttcatttct atgactagag tatatatcta aagaaacaaa atttcaatac ttttgttaaa
    21961 ataattaaat tttatttcta aaaattggat ttataatgcc aaaagaattg acacagccac
    22021 aaaaaaggca ctgctaagca aaagcagtgc cttgaaacat tttatttaaa taacacaaaa
    22081 gtctaattac tcataagaaa ttaattctgt attgtctacc atgaatgtaa agaacccttc
    22141 attaggcatt ggatgaaaat aagcattgat cactcttatc aaggcattat gacagactaa
    22201 caagtagaca ttttcttcgt cttccatgca ttctttcaat aaaggaacga tccgtgcgta
    22261 cacatctaag accgactctc catttgggaa acgtacagca aacgctaaac gcgctttttg
    22321 aaagttttca tctttgcttg gcagtccgtc ataatcccca aaatccattt ctaccaatcg
    22381 ctcatcagcg gtcatcggca aattgagacg atcaactact gcttgtgctg tatctcttgc
    22441 tcgttgaaga ggggaatgga tgattttcgt gattggaaca ggcaaatcta ctaccttttc
    22501 tgctagtgcc tgtgcctgca tctgtccttt ttcagtcagc ggcacatctg ctcgcccgca
    22561 aactaatcct tgtgcgttcc aagttgtttc tccgtgtctc gttacataaa gcatgtgatt
    22621 ctctccttcg ttgacaaagt tctccttaac cataccttgt tgtctttgag attacaaggt
    22681 ttagattttc tcaagagaaa taatctcagt agatacttta tctagaaagg tttgatcatg
    22741 ttccactagt aacatagcag gtttcacgtt caaaatcagt tgttctaatt gttcttgatt
    22801 gaagacatcc aaataattca atggttcatc ccatatatag agctcagctg gttgggataa
    22861 agatttagcc aattccactt ttttccgttg tcccatactc atctgttcga ttttattatg
    22921 aaaaacatct ctttccatcc caagctttcg gagattattc aaaaatgctt gatagtctac
    22981 tcggtttttc ttcgcgaatt ccgccaatgt ccctcgattg tcttcataat tttggcgtac
    23041 gtaactaatg gtcaaatgtt tgggctggga tttttctcct acaaccttgc cgttgaatgt
    23101 ccccagaaga taatggataa tggatgactt tcccgcgccg ttaggacctg aaattgccat
    23161 ccgctgatga ggttcgatcg taaaatgaac tggctcgaat aacagatttt cataccctaa
    23221 ttgaagattt tctacgctta acagccgttt atgatgagac acttggctat tcatcgtcaa
    23281 agcatcgata tactcgatat cttttaataa tttttctttc tcactgatct gggtttccat
    23341 ccgattcacg attgctttag atcgtttcat cgtccgtgca gcatcagcgc ctactgctcc
    23401 tttattcact cgcctagatt ccgtatcgat gaatccgact tttttctttg ttttgtcccc
    23461 ttctcgggaa cgagaccatt ctgctttttc agctgctgtt ttctttagcc tgctgacttc
    23521 ttttttcaat ttttcatttt tagctatttc aaattcatca cgaagttttt tctgttcttc
    23581 atagatagag aaattccctt gataaagttc cagttggctc ttttcgattg ctaaaacatg
    23641 gtccactact tcatcgataa atccccgatc atgactcacc acgatgaatc cttgtttttt
    23701 ctttttcaag taagccgcta cttgttttct gccagagata tccaaatgat tcgttggttc
    23761 atcgattaac gggaaatgag tgtcatccac aaataagagt gccagtagga cttttgtctt
    23821 ctctcctcct gatagtgtgc tgaattctct ccataagatt tcaggatctg tttgcatcag
    23881 ttggagctct ctttcaattt cccatatctc aaaatccata atctcattta acacataata
    23941 ggtcaaacgc tctttatctt ttgtttgctg cgggaaatag acaaacgctt gctgatggat
    24001 cacttgccct tgataaggga gtttgttttg caggatattc agtaaagttg tctttcctcg
    24061 accgtttcgt ccgataagtc ctagtttcca ttgtgtgtca aagtttagat tggcttgttc
    24121 aaataataat gtgccttggc tgtcatagcc gaatgtcaga ttttttattt cgattttaga
    24181 cattattcca tcacctcgta aaaaaagacc agtcctattt tcagaactgg ctaaagagga
    24241 tgcaagggtt ggggcacaca aaaagaccca tacatcgaaa gaaccaagcc aatcccgaaa
    24301 atctgcacac tgctcccttg tctccacaag gtacagtctg aatgcagctt tcatcaatcg
    24361 acttaatttt tcaatgcacg gatccctcac tttgtcctat atttgaaatt acgatagcat
    24421 aaagaaattg tttttgtcaa ctacttcggg agaatgttga aaaatctgct gagtgtctgt
    24481 cctaagattt ctgttttttc taatgtttgc ctgtgttcca ttgcttcctc taaatcgatt
    24541 ggtcctaatt gaatgcttaa gacaatgggg agaaaatcaa agagtccttc cagctctcgg
    24601 tctctacttc cgcaaatagc gattattggt tttcgatatt tttttgcaag cactgctacg
    24661 ccatatggaa cttttccgtg gattgtttgc tgatcaatcc gcccttctcc tgtaatgatg
    24721 acatccgctt gttcgatctg tttttctaat ccagtgattt ggctgaccaa ctcaaaacca
    24781 gagaccatct tcgctcctaa caaaaaaagg gctccgccaa taccgcctgc tgctccgctt
    24841 cctgggatgt catctaaatc cacctcccaa acatctttta tctgctgagc aatcttttca
    24901 gcttgtttat ccaagaaagc taacgtcgta tccgttcctc ctttttgtct tccaaattgc
    24961 caagcagctc cttgttgacc tgtatatggg ttggtcacgt ctgcagcgat gatcactttt
    25021 actttagaaa caagaacgtt ccttcgatcc aaatgaagtt ttctagtaga taacaaagga
    25081 tttccttctg tgtacccttc taaagaagca cccaaagatt ggagtaatcc tagaccacca
    25141 tcgctacatc cgcttcctcc taaagtgaca atgatttgtt ccacttcttg aagcagtgta
    25201 tctttgataa gctctcccaa ggcataacta gaagcaagtc ggatggtttg atccgttggt
    25261 ttatcaagta aatctaatcc taatacttgt gcagattcaa tgactgcggt attttttcct
    25321 tgccagttcg tcaataaata actgattttt cgctgacgaa ataagagatc catcccatca
    25381 atcgtacgcc aggttccccc gagcgcgtaa tagattgctt ctgcagtatt ttctccgcca
    25441 tctgcaatcg gcacattgat ttttcttcct ttacggtctt cccaaccatt tagaactgcc
    25501 tgatttaatt caaatgaaga agcacttcct ttaaatgaat cgattgccgc caaaatatcc
    25561 atctatatcc ctcactttct tcttaagtaa gacacttttt ttcacttttt tcaaacaaaa
    25621 caatgctatt ttcttctgtt tttctatata ctaaaaggga actcattgaa taaaggagag
    25681 aaaaaacatg aaaatcgcaa tagctggagc cggagcaatg ggcagccgaa tcggattaat
    25741 gttgcatcaa agcggaaatg aggtcgtctt gatcgatcgc tggcctgctc acattgaagc
    25801 cattcgtaca aatggattga ttgctgactt taatggcaaa gaagtagttg caaaactgcc
    25861 aatctattca ccagaagaaa tcatcgaaag caacaaacac gtggatttga tcgtagcctt
    25921 gacaaaagcg aatcaattag atgatatgtt tcgttcgatc caatccatta ttactgagaa
    25981 tacttatgtt ttgtgtttac tgaatggatt aggtcatgaa gatgtactgg aaaaatatgt
    26041 accaaagaaa aatatcttat ttgggatcac gatgtggaca gctggtctag ctggtcccgg
    26101 aaaagtcaca ttattaggtg atggagaaat cgaactagag aacttggaac cagaaggcga
    26161 agcatttaca aaaaaagttg tagaagtctt tcaagaagca aaattgaatc caatctatag
    26221 tcataatgtt cgttattcca tttggcgaaa agcttgtgta aacggaacat taaatggatt
    26281 atgcacgata ttggattgta atattgctga attaggtgcg caaaaagcgg cagagggcat
    26341 ggttcgaacg attgtatctg agtttgcttc tatcgcagca aaagaaggaa tcacattaga
    26401 tcaagaagaa gtgtaccaac atattgcttc tacttatgat cctgacaaca tcgggttgca
    26461 ttacccatct atgtaccaag acctgatcaa aaaccatcgt ttgactgaaa tcgactacat
    26521 caatggtgcc gtttggcgaa aaggacaaaa atatgatatt gctacaccat attgtgcctt
    26581 tttgacccaa ttagtccatg caaaagaagg aatccttggc gcagaataaa aggctctctg
    26641 atgagaatca gctggatcag cagctggttc tcattttttt acccccatga aagttcaaaa
    26701 agaaaaacac aattctcatg ttgccataag aattgcgctt gtttattttc atctatgatt
    26761 ggttggcact tttatttatt tttaacagag gaaagatcgt attcatattg ttcataaata
    26821 gcggagtcag tagtgaaaaa acgttttgcc caattttctg catcaaaaaa ttccaacagt
    26881 tctgatggga ttggttgaaa tggcgtatgc aaaaattccg gtaattcatc taaatcacgt
    26941 tcttgaagca aaaaaatatt ttcagaatga taaaactgat aatgagctat ggcttgatta
    27001 tctgtcacta gtttcttttg aaaatacata gattcaaacg ttcgcaaagt cactccttgc
    27061 tgcccttccc gcaacagttc tagaatcccc ctcgattccc cggttttttt cagatattct
    27121 tcataagtaa ggtatccagg ttgttcattt cctcgaagcg gaagaataaa gtaattggtc
    27181 gtaacacctc tttttgtaaa ttcctgctta tatttttctg cccgttcttt tctgccgtta
    27241 tttgttgcac caaaaaatag atccgtcttt ggttcgctag aagaaaaatc catatgtttc
    27301 gaataaaagg aactattatg ttttagtcca agttctttcg cttctaaaaa atcaaaatga
    27361 taaatctcat cgattgccgg ttcatcttgt gcctgcttca gcatcgcctc gtgttctgct
    27421 ttaaagtggt tccaaaaaaa taacacgatt tttgcattgg taaatggacg cacgcgtcga
    27481 gccatctcca tatcaaatcc ataatctaaa aaaacgatca gctgataatt tgccaactgt
    27541 tgtcgagctg atttaatgaa ttccgactgt agtaaatctt ttccggacca aacatctaaa
    27601 gagcggatgg aaggaaaaaa ataaagtgat ttatttttcg caattagcaa tacgtcgttt
    27661 ttgttcatct gttccgcctc actttctctg tcactttttc ctatgatagc ctgtttaaat
    27721 agtaaaaagc cagtaaaacg cttcctatta tttttataaa aatttaacat ttctatgaaa
    27781 atacgatgaa ataaatcatt cttccattaa aatatgccca aaagttttct gaatagttgt
    27841 aattatctga aaatttgtta tcattagtca ttattattat tactatttat tcttactatt
    27901 tattcttact atgcattttg attgtgaatt taaaagaaag aggttattta tggaagataa
    27961 aaaattgaaa aaacaactta gctcccgaca tatcaccatg ctagctttag gtggagcgat
    28021 tggtgcaggt ctatttaaag gaagtggcga agcgattgga atcgccggcc cttctgtatt
    28081 gatcgctttt ttgattggcg gcataatttt atatatcgtg atgaaaggtc tgggaaaaat
    28141 tgttttaagt ggtggtgata cgcaccacgg attatctgga ttgattcgtc catatctagg
    28201 tgctcatgct gccgatttta ccgattgggt ctactggtcc atgtggatta tcaatatcat
    28261 tgctgaagct gttgcagctg cttccttttt acagttgtgg ttcccccaca tcccagcttg
    28321 ggtatttgtt tttatgttgg ctgttttaac tactatcatc aatctttatt cggtacgatt
    28381 attcgcagag acagaatatt ggctggcctt tgctaaaatc agtgtcatta tcctattgat
    28441 catttttgct gcttatcttg tcggtcagca aatgttgggt actggtgttt tccctaccct
    28501 tcaacaatta actgatcatg gtggttttac tccccacggt atgaagggaa tcatcagttc
    28561 tttgcttgta gttatctatt cttacggcgg ttctgagttg attgctatca cagtaagcga
    28621 agcagatgat cctaaacgtg cgattcctaa agcaatcaaa ggcgtcatgg gaagaatcat
    28681 ttccttttac attattcctt tatttttgct tttgattatt tttccttgga atactttagc
    28741 aggcacgaac gttagtcctt ttgtgatggt atttgaaaaa atgaatattc catttgcggc
    28801 agatatcgtc aatttcgtga ttgttcttgc tctgttctcg tctatcaatt caggtgtata
    28861 tgcttcttcg agaattttgt tcttccgctt gaaagatcgg aaaggttcct ctagaaaact
    28921 agctgtttta aacaagcatc aagtaccgca acgtgctgtg ctgttttgta ccagtgttct
    28981 ttatctaggt gtggctcttt cttattttgt cggtgacaaa ttatttggct atctggcagg
    29041 ttctctttcc tatacagtat tattgatctg gatcattatc agtgctgcgt cctttgtact
    29101 gacactaagg aaaggaacgt tatgggacaa aggaatcagc ttttttgcat tagcagtctt
    29161 atggttgatt ttccttggta tcctattcac gaactccatt ggggtaactg tattgaccgg
    29221 tttgctttac ctgttcattt atttcagtta tcaaaaaaag aatgatgctt tcgtcttgac
    29281 gaatgaatga aaccaataga gaattttatt ctatttatat tgaatatctg gagctattga
    29341 aaaaaaggag ctgcgacaaa aattgttgca gttctttttt ccgaataaac ggtgttcaaa
    29401 aaccatctgt gcagattaat tgattgttct ccacgctcat gacatgatag aagaaaggaa
    29461 tgaagaaaaa ggagtttgtt ttaaatgaaa aagtattgga agtttttgtt gatgattggt
    29521 gtttcgacgg tagtcatgtt tggtatgatg tatttgaatg tttatgcgct tgatcatatc
    29581 ttcttcagtc gcacacgtgt attcatggcg ttgatgatgg gtgcagtcat ggcagtcatc
    29641 atgttgttgt tcatgtggaa aatgtatgaa aacaaaaaga taaatgctat gattttagcc
    29701 gctagtcttc ttgtttttgc cggttcctta tttatggtac gaagccaaac aactgtcaac
    29761 gatacggcgt ggatgaaggc gatgatccct catcactcta ttgctatctt gacaagtaaa
    29821 cgagctgatt taaaagatcc tgaagtaaaa aaattagcgg aagaaatcat tcaggcacaa
    29881 gaagaagaaa tcgacaaaat gaaaaaaatg atcaaagaaa tggaatgacc attatccatc
    29941 caaataaaaa aagaggatgt tccagaagtg atcaacttca agaaataaga agaagctact
    30001 tctggaacac cgtgaatcag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
    30061 gccaaacaaa aatagcttct catatttttc gtttggttag gcttattacc gcaaatgcaa
    30121 gcttttgaac accgtttatt cgtaaaaggg agtgtgacaa aagtcttgtc acactccctt
    30181 tttacgtttt tattgttcta attgcgctgt cttgcttact gcttcttgcg gtttcacaaa
    30241 tttctttgag atcccccaac ctgtaaaggc actgaccatc gaaaacacag gtgaaagcaa
    30301 actgaaaaac acgaatggca agtaagtgag tgtaggaatg ccaaatgtac ttgctacgaa
    30361 acttccggcg attccccaag gcactaagta gttgattact gttccaccat cttctagtgt
    30421 tctccctaat acagttggat caaatcctgc tttttcgtaa gtttctttaa atgcattacc
    30481 aggcaggata acagatagaa attgttctcc aataaagata tttactccga tgcttgtcat
    30541 caatgttgta aaaatcaatt ttggggtgtt cgtcatcttt cttgatactc gttccataac
    30601 ggttgagatc aacccaaatt cgatcaacaa accacctaat gacaatgtta gaataatcaa
    30661 tgacacagtt ggcatcatgc tttcgattcc tccacggcta agtagtgaat cgatttgttg
    30721 gtttccagta tttgaaacaa atcccgattc gatgacccct gccaacttcg ttaaggaagt
    30781 ttgtgggttt tgaataaaga tcattcctac tgctacagca atattcaaca aaatcgtcaa
    30841 aattgccggt acctttttcc atgcacacaa gagcattaaa aatagaggaa tcagtgacca
    30901 gatcgaaata gtaaagtttg cttctaaaat atcagtcact tcacgaatag atgaaagact
    30961 tgcggcttta cttgtgtgtc ctaaaatcat gaataaaaag aaagaaacaa caaatgccgg
    31021 aacagttgac cacatcatat gcttgatgtg tttgaacaaa tcggcatcaa caatagctgc
    31081 tgctaaattt gttgattcgg ataatggaga catcttgtct ccaaaaacgg cacctgaaac
    31141 aatggctcca acaacaaggg ctggatttag tcctagtgtt gtcccaatac cgaaaaatgc
    31201 aatcccaatc gttgacataa cagtaaaggc actccctaca gcgctaccta cgattgcaca
    31261 aacaacaaat acagaaggga cgaaccactg gacactaatc aatttaaatc caatgaccat
    31321 gagggttgga ataattcctg cttgtatcca tacagcaatc aatgaaccga ctaaaataaa
    31381 aataaagatt ggaatgatcc ccggtttgat gccattgatg atcccttcgt gcagccaatc
    31441 tgtcgggtat ttttttatca ctgcgtacaa catgaccatt ccgatagctg ccaaaatcgt
    31501 gatattaggt gaaaccttta atccgacaac gccaatcgca atgcctaaaa caatgaccat
    31561 caatacaagt gttgcttcta aaaaacttgg tgttcctttt ttttccatga taatctctcc
    31621 ttttttagtt gttcttatgt tctaaaaaag ttaagatatc ggattactcc gccacaattc
    31681 atccgcgatt cctcctttat aaaaataaaa agacctgctt tcaactaagg acgaatgact
    31741 ccgcggtacc accttaatta aaaacagatc tgtttttcac tttcaaacga ttattcaaat
    31801 gtatggactt tccatgtaat tcgtcaatta ggcttgatcg gttcacagcg accaccgact
    31861 ttctgaacaa aacacctaac cactactgcg tgaaaagttg acttgtatga agtttacctt
    31921 ccataagatc atttgtcaat gaaaaagaaa atattttttc atttttctga aaattatttt
    31981 tataactttc gcgcacaact ttatcttttt actagaaagt agcgccctct tgtttttttc
    32041 ttgctactat ttcttgatgt tcttgtttga ttttttctaa tagcttgggt tctctcaata
    32101 aatctagagc tgtattggca agtaattccg ctgcaatcgc aatagaagca agtccttttt
    32161 cacttttagc agcagctttg aattcttctg tatgtccggc aatatatgtc tcagagattg
    32221 ctacagtagg ctgtatcgtt ggaataactt ggctaacatt tcctacatca gaagaaccta
    32281 gacttgttct ttgttccgtt tctatttctt catcaggaac ccctgcttct tttgcatgtt
    32341 ggaagaataa gtcatcgaaa gaaggcgtta cgatgacatc atcgactgca ttttggaaca
    32401 atccaaactc aaaagtcgta cccgtgctta atgcagcacc tcgtacgata ttttccactt
    32461 tttcatatac tttgtctaat gtttgtcgat ttcctgcacg taaatagaaa cgtccagcag
    32521 cgtagtctgg tatgacattg gccgccacgc ctccatctgt aatgattcca tggatattga
    32581 catcttttgg caattgcaag cgtagagcgt tgattccgtt aaacacttgg atcaaggctt
    32641 ccaaagcatt tactcctttt tcaggtgctg ctgctgcatg agaagcgaca ccgtaaaatt
    32701 tgatatcgac tggatcattt gctaaagatt ctgtcgtttt cccataacga aaagctggat
    32761 ggacacataa tgctgcatcg acatcctcaa aaaaaccttc ccgaacgaag cttcccttag
    32821 ctgatccatt ttcgcctcct tcttcccccg gagtaccata cacacgaatt tctccgccaa
    32881 tctcatcaat gaattgtttc actatgctgg ctgctaatac ggagtaagta ccaaataaat
    32941 tatgtccgca tgcatgacca atacctggta aagcatcaaa ttctgccaaa aaggcaattg
    33001 tcggacctgg tttattggaa atataacgtg catcaaagcc tgttcggtgc ccagctactt
    33061 ctttttctac ccagaaccct tctttttcca attgtttgat taaagctgct gaggaataaa
    33121 attcataatt acttacttca ggatggtcgt ggatagctag tgcaagtgct tgatattctg
    33181 gtaaacggta ttgaatcaga tccttcaatt gatttgttga gagtgtggtt gtcataaaaa
    33241 tttcctccta tgctagtcgt tttttcttat tttctcccga atgtttccca cgcaggtacg
    33301 cttgaacctt ttgatgtttt ttcgattact ttggctgttt cttctgtttg ataggcatct
    33361 actatttttt gatacgtttt attgtcttca tcttcttttc ttgcaacgat gatattgaca
    33421 taaggacgtg catgctcatc gactggttct aagaagattg catcagaagc gggattaaat
    33481 ccagcatcta cagctacacc gctattgatg actgaaatat ctacatctga caaagcacgt
    33541 gctgtttggg aagcatctaa ctcagaaatg tctagatttt ttttattctc ggtaatatca
    33601 gaaacggtag gcgcttggcc ttttgccttg tccactttga tcaatccagc agtttgcaat
    33661 aacagaagag ctctgccgcc attcgtgaca tcattaggga ttgcaacttt tgctccatct
    33721 ttgatatcag ccacatcttt taacgtattg gaataaattc ccaacggtgc attaaccgta
    33781 ttcccaatcg atactaaatc agttccgtgt tcttcattgt aattgtctaa aaagaattga
    33841 tgttggaagg aattcaaatc gatcgatcca tctgctaaag ccgcatttgg ctgattgtaa
    33901 tctgtaaatt tcacatattc caaatggatg ttttcctttt tcaaacggtc tttcacatcg
    33961 tcccatacat ctgtatcttc cccaatgatc cctagtttta cggtcgtttc ttttttttca
    34021 gttgtagctg cttttccttg gctgcatcct gccaaccaaa taattgctac aaatgtaatg
    34081 actgcaaaac gaataatttt tttcattttt tctcctccat tttcaatgtg tgactttttt
    34141 aatcagttga ttactgataa attgactcaa aaaaactaag accagaatca ataatgtagc
    34201 gacaatcgtt acatctgttt ggaatcgatt atatcctctc gatatagcaa gattccctaa
    34261 tcctccggca cctactgctc cagccatggt agtcagtccg atcacattga tgatcgtcaa
    34321 cgctgagacc cgaatgatac caggtaaccc ttctaacaaa taaacacgta aaatgatgcc
    34381 gattgggctc gtgcccatcg cttctgctgc ttcgatcacc ccaggatcaa cttctagcag
    34441 tgcattctcg atttgacggg caaaaaacgg gacaacgcca aaaatcaaag ggactaaagc
    34501 tgctttggca ccaatcgtcg ttccaactaa gaaacgagta gtcaaagcta aaagagaaag
    34561 caagataatg aaagggatgg aacgaatgat attgatcaac ttatcaagta tttcgaacag
    34621 cagcctgtta gctagtaatc catttggacg tgtcactact aacgtcacac ctaagatgat
    34681 tccgatgatc ccaccaatga atgccgtcca aaaagacata tatagtgttt gctttgtcgc
    34741 ttcgatgaat tcatccggaa tcaaatagac attcggcaaa tatgttttaa aaaattcagt
    34801 cattttagtt tcctccttcg atgactttga tacggttatc taattcattt ttttgtaaaa
    34861 tagttacttt tacaccagat ttaatcaaat aatccaatgc tttttgccgc tgtttggttt
    34921 ctccagctaa agtaacgatc aaacttccta agggtgtttc ttgtaatagc tctacattac
    34981 cgtataagat attcgctgtt acttggtact tgctgtaaag atgagcgatt agaggttcgt
    35041 tcgtctggtt gccgatataa cttaattcca ccaaccattt atttgcaatc tgatcggcaa
    35101 aagcagcatg ggcactgatt gtttccaatg cttggtcaag gtgtgtcgct gtccgaataa
    35161 aatctttagt caattcttct tcaggatgac tgaaaatctg gatgctgcta cctttttcaa
    35221 tgattttccc cgcttccata accgctacct tgttgcagat ttctttgaca acttgcatct
    35281 catgagtgat caggacgatt gttagaccta gctgacgatt tagttttttt aacagtgcaa
    35341 ggatctgaag ggtcgttttc ggatcaagtg cactagttgc ttcatcgcat aacaacactt
    35401 taggttcatt tgccagagct cgtgcgattg ccactcgttg tttttgcccc cccgacagtt
    35461 gtttaggata cgcagatgct ttttcttcca gaccgacaag ttccagtagt tcattcactt
    35521 tttgacgacg ttcctgtttt gattttccag aatactttag agaaaaatct acgttgtcaa
    35581 aaatcgttcg actttccatt agattgaaat gctgaaagat cataccgatg gcctttcttt
    35641 cttttcgcaa ttcttttgga gagagctggc ttaaattcgt ctgattgacg atgacttctc
    35701 ctgctgttgg tttctgcaac aaattcatca cgcggactaa cgtactcttt cctgcacctg
    35761 agtaaccgac gatcccataa acatcccctt tttcaatcgt taaatctaca gaatctactg
    35821 cctgtatctc tttttctttt tgtttgaaac atacagaaat atctcgcaat tcaatcatta
    35881 acattcctcc ttgtctataa aaaaagcatc ctcaagaaaa tgaaaatagt cattttcttg
    35941 aggacgcctt tttgcgtgtt accacctcat gttcaagctg tgttcacaca caactcctct
    36001 tcaagtacca tcatactcta gcaagatatc ggtcgcagcc gtcaccgcct tacatctatg
    36061 ctcgggatga attgctcgat ggccattttc caatagtcgt tccttgcttc ttctcagcta
    36121 ccgaagctct ctgtcaaagg ttcttctatc gtacttacca tttcaatgca aataatattc
    36181 aaaaaaaaaa tccttttact aacataaact atgtcagtaa aaggacgacg tatcgtggta
    36241 ccaccttttt tcggaagttt ccttccctca agcccaacat ctcaaaaaga gagattggcg
    36301 ttcgttttaa cggtcgaaca tttccgaatt tgcctactta tcgacaaatt attctccaag
    36361 gccattttca gcttttctta cttgactctt cacaccagcc agagcctctc tgcaaagttt
    36421 ccaagcttac tttccttctc aacgaaatca atattttgtt gttgggaata atctacagga
    36481 ggaaaactga aaagtcaagc atgaaatcca aattattttt tctatattta tttttctgtt
    36541 tttttatcta acatttcaat aacagttcga agatcttttc cttccgcaag aaggttcaat
    36601 ttttccttct cttcgacgct ataatttcga acaaaactgg aatcgggaat caaaacaacg
    36661 cctagtcctg cacgtgaagc ggctgtcgct cctactaaag aatcttccac tgcaaaagct
    36721 gaaagagctt ctaatcctgt tcgttccaaa gctgtcagat aagggactgg tgaaggcttg
    36781 ttctccgata cttcatctcc gaacgtaaac gtatcaaagt aatcggctaa ttgaaagtga
    36841 tccaagatat ctgttgctct tttcgtaaca gttgtcgtag caatccctag tttcatatga
    36901 cgttctttta agtctgtcaa tatttctttt gcatagggtt tcaattggat agctccactt
    36961 gtcagctgtt gataaaaata ttcttcacgc tttgctcgga cttcctgcac tttttcagga
    37021 ctgcctgctg cctgcactaa aatatcaaaa ctttgtttcc agttttttcc gccccaagcg
    37081 tcaacgattt ctttcgggat atgaatatca aattgctgga atccccaaag ccaaccatcc
    37141 cgatacgttc tttccgtatc cactaacaaa ccatctaaat caaatattgc acctttcatc
    37201 tatacattaa tcctttccaa aacaatacac ctagtttagc atgactgatc caaaatgaac
    37261 agaagcatag agcccagccg catcacttat aagagttctt ggaatcgacg gatatagatt
    37321 ttcttcacag ctgtcacaag cgccagataa gcgacgactg ttataaaaaa ccataaccag
    37381 taattgccag ggagcggcat caagcctaac tcagcaccaa atcctgtgaa tggaaggact
    37441 gaaccgacaa tgattcctaa tgtagtgata accagcatgg caaatgttgc attgctttgg
    37501 ataaatggta ttttcggtgt acgcaatgca tggagtacta atgtttgtgt ccacagggat
    37561 tcgacaaacc atcctgcatg gaataatgcg ataaaaatca cttgttgtga gctatccaat
    37621 gtatggaaac ttccaccaac tacagcagga caaataacaa aatacatcaa caagtaagta
    37681 gtgatatcaa aaatcgaact tgttggacca aaataaacca tgaattttcc aatactagat
    37741 gcattccatt tctttggctc atccaaatat tctttatcca tccgatccca tggtaaggaa
    37801 atgcaagaaa cgtcatagat cagattcaaa aatagaagtt gcaaaggcaa cattggtaaa
    37861 aatggtaaga acgtactggc aatcagtacg gaaaacatat tgccaaaatt agaacttgct
    37921 gttgccttga tatatttcat gatgtttcca aaagtttctc ttccagaaag aaggcctctt
    37981 tccaaaatcg tcaaatcttt ttctaaaagg atcacgtctg ctgattcttt tgcgatatcc
    38041 acagctgtat ccacggaaat accgatatca gccactttca ttgctggagc atcattgata
    38101 ccatctccta aaaatccgac agtatgccca tttttacgta aagctgtcgt caatcgtgct
    38161 ttttgctgag gattcaattt tacgaatact tcgtgttctt cagctacttg cgctaattca
    38221 tgatcattca tttccgagat tttctcacca gtaatcaatt cattgatttc taggcctact
    38281 tgacggcaca cagaccgtgt gactaattca ttgtccccag tcagaacttt tacagctaca
    38341 ccatgttctt ttaaagcttt caacgcttgt tttgttgttt cttttggtgg atcaaggaaa
    38401 gcaagataac caatcagaac catgtctgac tcatctttga ctgaaaattc tccaacaacg
    38461 cttggattgg tcttttgtgc gacaccaatc actcgtaagc cgtcttcatt aagtgattgt
    38521 acttttgcaa gcaccgcttc ttttcgttct tttgttaatg gtgcgacagt accaccaaga
    38581 tcaataaatt tagaaatgtt caacatttct tcaatcgcgc cttttgtgat catctgtgtt
    38641 ttgccagccg gatcagcgac tacaacactc atacggcgac gttcaaaatc aaaaggaatt
    38701 tcatctactt tttgataatt gattgtatct gtatctaaag tttctgctgc ttcatcaatg
    38761 atagccacat ctaacaaatt tttcagccct gtttgatagt agctgtttag ataagcatgg
    38821 cgcaaaacac gatcatcttc atttccttca caatccatat gatattcaag aatgattttg
    38881 tcttgtgtca atgttcccgt tttatccgta cataacacat cgatcgcacc aaaattctgg
    38941 atagcattta aatttttgat gactgttcct tttttggcca tcacacttgc tcctttgacc
    39001 aaattcgtcg taacgatcat tggaagcatt tcaggcgtta aaccaactgc cacagataat
    39061 ccaaaaagaa aagcttctaa ccaatctcct tttgtaagcc cattgataac gataactgct
    39121 ggagccatca atgacataaa tttgatcagc agcatggatg ttttgtggat gcctaattca
    39181 aaacttgatt ggatgggttt ttcagatagc gtttctgcta catgcccaaa tagtgtgtca
    39241 tttccgacag aaacaatgat tccttccgcg cttccactga tgacattgct tcccataaag
    39301 acaagatttt cataacttgt ttcactttgc tgatcgtctt cacatacttt cgcatgcttt
    39361 tctactggat agctttcccc tgtcaatgct gcttgagaaa tgaatagatc ctttgcggac
    39421 aataaacgca tatccgctgg tatcatatct cctgctgaaa gccgaacaag atctccacag
    39481 acgatttctt ccataggaat ctctgtatac tcgccatttc gtttgacagc tgccgttact
    39541 ttcacaagtg atttcagttt ttcagctgct tgattagaac gcacagactg gatcaacgtc
    39601 atcgtcccac tgacgataac catagtgaag ataatggcta caccaaagag atctttatct
    39661 cctggagcgg ctaatacgta ttccgtaatg aatgaaataa ttcctaatgc aatcaacact
    39721 aatgtaaagg gtgtgatgta tgctttcaat atttctataa tgaatggtgt ttttttgcca
    39781 tatgaaatcg cattttcacc ataagtttct cttgaacgct cgatttgttc ttctgacaat
    39841 ccatttggtg atgcatggaa caattcaaat aattcttgct tcgtttttcc agcaaaagct
    39901 gcatagattt tttgtgtatc tttctttttt tccatctcga tctctccttt gtgtccaaaa
    39961 gacagctgtt ctcgctgttt ttggtctttt gctattcatc ggatcgatag agaaaatgct
    40021 gaaaggggct gagaacagat agataatagc cctggccttt cctctaaatc cttgtttttc
    40081 gaatgactgt catccatttg ttctcatctc ctttactaag taattttaat tcatgacttc
    40141 caatcatttt tcgaatagtt attttcccaa ctcgctcacc tccctttttg ttttttcacc
    40201 tatgggattt gcggcaacaa aaaaacctct cgccaaatac tcagcgaaag gtttgaaaaa
    40261 gcaaatcaaa aaaagcttct cagatctcat cgttgagttt tgacactgta caacgtaaaa
    40321 agcagatatc tgcttttagc taccttatga aaagccttat ctcgacaatt cctgttctac
    40381 ccattggcgt ctctcgacgt ttttgggcag tagcctatgt ttgtatagga gcctcaccta
    40441 acaaatccta ttcggttttc cctcgttaat ataacaacta aaaaagagga tgtcaaatca
    40501 attttccatg tttttcatac ttttttaaaa tacacgcgtt atatttcctg tctcacgcag
    40561 ttttatttgt ctcattttcc acctcccgct ataatagaat tatagtagga aacgaggtgc
    40621 tgttatggaa cgaacacttg ttattatcaa gccagacggc gtaagacgcc atttggtcgg
    40681 aagcatcatc caacgttttg aagcaaaggg actggccatt gctgaaatga aatttgcaac
    40741 gatgacacct gaactagcaa aagaacatta tcagcacttg actgaacgta gtttttttga
    40801 tgagcttatc gattacatga cttctggtcc ggtcgtttat cttgtgctgg tcggcgagga
    40861 agtgatcgat atcgtccgta agatggtcgg agcaacaaaa ggggcggatg ccgttccagg
    40921 tacgatccga ggagattatg cactgccggg gactgaaaac atcatccatg cttctgattc
    40981 ccgtgatgct gcaacaaaag agatcgcccg ctttttcccg ccattcgata caaacaaacc
    41041 gtctgaaaaa gcggcgaagt gatacaaatc ctaaactgtt ggtctaactg ctgggtttag
    41101 caatggccag cagttttttc tctaaagaat tcctttgatt cttccctaat gtatattcga
    41161 tcactgcgaa aaactcttgg tgattttccg atttttcttc ccctaaagca gcagaaatca
    41221 gtcgttggta ataatccgcc atcaatgttt cataaatcac tgtacttctt tctttatgcg
    41281 ctaaactccg tttcatatct tcattcacgc tacgtgcctt ttttaagcga ccattttcta
    41341 tataaaacaa agtcaagttc gtcaaaaaag taaagagata ctgctcgtga ttattcagtt
    41401 gcgcgatccg tcgcattctt tttttgattt tcaaataatt attctcaata aaacgatcac
    41461 tgaatatcca aggaaaatct gctagaatag atacttcaaa ttgcccatac tctggtactt
    41521 gcatcaaata ttcttcaatg atacgacatt cataacgcaa aaattcaaat tccgtctcag
    41581 atacttctgt ttttttggac atgtgcagat tcaaaaccaa atagtacaga tcttctgttt
    41641 tcttatagtg cctgcggtta ttagcaattt ctttgtaagg atcaaagctg tcttcttgaa
    41701 tccgatctag cattcgtctt tttttcttta atttttttgg ctcatcaagc tgatttcgta
    41761 tccattcata ttcggcaagt gtcatgttca ttcgctctaa aaatgatttc aatgttccaa
    41821 acgatacttt tttcactgaa tttgtctcga tcccgactag cgaagtttta gaacagattc
    41881 cttccgctaa ttgggtctgt gtcagctttt tgttgactcg aagcttctta atcaattctc
    41941 catcatacat aatagttctc cttttttaat tgaaattaac ccttggcttt tccagaatgc
    42001 ttgccaataa gaggaatgat tatttcaatt taaattttcc gcattcaata aatagatacg
    42061 ttaaaaataa aaaatacacg aaaaaaataa aaaaatgttt ttctcataaa aaataccaac
    42121 cttcaagcat gagactgctg tctaatccct gaaggttggt ttgattgttt attgatcatt
    42181 tttttactgc tccgcattgg ttcctgttac tggatatagg atagtcgttg ttttattgcc
    42241 gttttttgaa aagacactgg tcgaatgttc acctttttgc ttttcgattg cttcattacg
    42301 ttccgcttcg tttttatagt catatttttt cggatcaatc gtctcaaatc cgtttggcac
    42361 ataaaaccgc aagagatttt cctgattcag cttatctgaa agacttaacg cagtcttgac
    42421 tttttcctga tctttggaga tttcctcgtc gattcctgct gctttcgtat cgatgatttc
    42481 tcctgtctga ttattgtaag cttttcctcc taggacggtg tatttgggcg tcacgaagtt
    42541 cccatttcga aaagcaacta cttgatcgtg ctgagaagat aataagtctg taccaaaatg
    42601 aagataatct ttatcatcga ttcccaatag atgtagtaat gtcggcaaaa catcgatttc
    42661 tccaccatat tcatgattga ctgtcccttt agtgtaacca ggcatatgga tcatcaatgg
    42721 cactcgttgc atctgtgcgt tgtcaaattc atcccatgta tctggatctt taccaagagc
    42781 acttgctaaa tctttattat ctgtattcga gatcccaaaa tgatccccgt aaatcacaaa
    42841 gattgaattt tggtaaatac cagatttttt caaatacgta aaaaattgtt ccaatgattg
    42901 atccagataa tgggccgttc gaacataatt atctactgtg ctattgccag tattcaaaga
    42961 tggaaagtcg aaattcttgt catccgtata ataaggcgtg tggttggtta cggaaagaaa
    43021 tttcgtgtaa aacggctgtt gtagatgttc aagatattgg gcagattctc tgaataaatc
    43081 tttatctaat atcccataac ctaacgtttc atcggattca tcaaaataag atcgatcaaa
    43141 gaaattgtca taccctaaat ttttatatac atgatctcta ttccagaaac tagctacatt
    43201 cccatggaaa acagcacttg tatagccttc tgtttgacca agggtcgctg gtgctgcttg
    43261 aaagacatta tctgacccaa gttctgtgaa taatgagcct tgaggtaaac caaatgtgcc
    43321 agtttccaac atgttttcag catcacttgt ctttccctgt cctacttcat gaaagaaatt
    43381 gtcaaaactg atcgttgctt gattctggaa aatactattt aaaaacggcg tgacttcttg
    43441 tccgtctacc ttcatattga tcaaaaactg ttgaaaactt tctaaatgaa ggacaatcac
    43501 attttttcct ttagcaatcc caaatgtttc aggatttggc tctgcataat gtttctttgt
    43561 atagtctaaa acttcatcaa ttccattact gcttgcatgc gcgcgaacac tgctagtcat
    43621 tcctgttttg attccgtcat aaatagtgta cgcatcgatt cctagatatt tgacaatata
    43681 ggaacggtca aatgtccgct gtagcaattg gggacggttg gcttcgctca acgctaaatt
    43741 tcctgaaaaa atcaatcctg aaagacaggt aacagcgata gccatcggtt tgctgacagg
    43801 gttgcttttg ataggtattt tcttgacctt caaccaaacc agtagcccaa tgaacacagc
    43861 gatatccaac caatagatga catcatgggg tttcatcaaa gaaaaggagc ttcctgacaa
    43921 cccttgtgac actttcgaaa agcctaatac agatttgact gtaatgaaat ctgtgaattc
    43981 ccgataaaaa ataatattca agtacaacaa aagcgtattt aaaatatcaa tgatcaaaag
    44041 aatggctaac gctggctttg gctttttaat atacaaagaa aggccaaaaa acaataaggt
    44101 agtcgcaatc gggttgatcc acaagataaa gtactggatc gtaccttcca cacctaatga
    44161 aaaatctgca tagtaggcaa tgatcgtctt cagccagaaa aagaaaacag ctgtcaaaac
    44221 gattgctaaa cgattttggc acaatttttt aagttgtgtc acgtatatca cttctctcta
    44281 ttcacatttt ttcttatttt gctcctgaaa aatgaaaatt tcctaaaaaa gttgttatct
    44341 taagattttt ttattctttc taaagattat tccattcatt tttcacagca taaaatcgtt
    44401 tcggtgtaag cggttaaaat agtaaggaga taaaaatctt gtggtgaccg atttactcta
    44461 tagtattcaa aagtagtttc tcggtattcg tacaaaaaaa acatactaag attagagggg
    44521 aacatcattg acgaatgatg ttctcctcta ttttctattt ttagattata gtgaagagtg
    44581 aaagaaagtt cgaacaaagc cttgaggact acgagtccct atcaaaaacc gaacttcttt
    44641 actgtcattt tagaatcagg tttacgtatg ttggggccat gagcccgtgt ataggaaatt
    44701 ggaatcgaaa tgaagcagta aagttctttc aacacttaaa aaggaggaaa acaaatgcct
    44761 tacattcttg cattagatgt ttcaatggga cacagctaca gtgtcctata caaagacgac
    44821 acctgcttat ttgaagacga gatcgaacac actcaaagag gatttcatgc attactcgaa
    44881 gagatccagc acttgcctga acgtccattg attgtttttg aagcaacggg tatttattcc
    44941 agaccagtcg aaaagttctt tcaagatcat cacttttctt actgtttgtt aaacccgtta
    45001 gaagcgaaaa aacagatgga agaaaacacc ttacgcagtt ggaaaacaga taaatcggat
    45061 gcccatcggc tcgcacagac tcatcttaaa aatcaacgtc aaccaaaaga agtacaaagc
    45121 aatttttact tagagatgcg cgatttagcc cgtttttatc aagaaattga gaagaaaatc
    45181 actcgactgc gcatggattt acacaactgc cttcagttga cttttccaga gcttgaacag
    45241 ttcttttcaa acaggctgac cccttacgca ttgacattga tccgtctttt tccccatcca
    45301 gattttgtat tggcatctac tcggacaaaa ataaaaaata aactgatcaa cgaaacacgg
    45361 gaaaaaatct ccgcgaatcg tgccgagcaa aaggcagatc aaatcatcca ctatgcacag
    45421 tgtgcctatc cagctgttga aaaagacagt attcactgtc aaaaaacgat ctattacgcc
    45481 gaacttctcc aagatttact cgaacaaaaa aaagcactgg cgacacaaat gatcaaaaaa
    45541 gccgaagcct ctccttgctt tttgttgtac caaacgttcc ctgggattgg cgcactcaca
    45601 gccgctttat tgctcggtga attaggagat atcacacgat ttaaaaccca taaacaattg
    45661 aatgcctttg tcggaatcga catccgacgg tatcattcag gtaagtatac tggccaagac
    45721 cgcatcaata agcgtggtaa tcccaaagca cggaaaatta ttttcttcac cattcgaaat
    45781 atgattcgcc agcaacgcgc agctcctaat catattgtcg attattacta caaattaaaa
    45841 aagcaaccta tccctaagaa agataaggtt gccacagttg cttgtatgaa caagcttctg
    45901 aaatgtatgc acgctatggt tagggcacat acagagtacg actatgcgta cgcggtctca
    45961 gtggaccatt aactaaacca cttacagtat accacttctt tgcccagatc gaaaattttt
    46021 tacgaacggg gctttattta gtgtgccttg attttgtcgc tttcgtttac gagctatttt
    46081 tcttgcacct tttacttgac taatcgtagg aaagaggctg tgacaaaatt cttgtcgcag
    46141 ctccctattt cttgatgcag gacagctttt tcacaacctc tccactaaac ggtgttcaat
    46201 cagctgacct tcggtattag ctcaaaaaac ggcaaaatat gaggagctat tttcccgttt
    46261 ttttctctaa tactcaggtc aaaccgctga tttcacaacc tctgattcac ggtgttccag
    46321 aagtagcttc ttcggaaata agccgaaatt ttacaaaaac ttgagaagca cttttcgtaa
    46381 atttcttctt atttcttgaa gctgaccact tctgtcacaa cttctgtttt tcattgtcca
    46441 taggtttgat attgtccttc tgatgactca ttcgtaatga ttgaggcttg attttcttca
    46501 tttcccgtta tcgtttgtcc actgccagaa tctgaagggc ttagttctgt cgactcgttt
    46561 ttttgagaag aagcagttgt tttcttgact tgttcgttgg tctttttcca ttccgctagt
    46621 cgatcggcca ccactttttt gtccactttt tcagaataag ctgtaaccac ttttccattt
    46681 ttcaaacgtg ctaccttgtc gccatcatga gtcaaaagca aaacgaccaa atgtttgtct
    46741 ttgaattgat attgtctgag agaaatcgca ctaagtacgt catcgatatt gataaattga
    46801 ttttcttctt ttccttttgg caaatagatg tccaattcac ctttttcgta attggcagtt
    46861 aatttatatt cctcccacca ataagttgag aattcttttt tgaaattatc tactgattct
    46921 ttgcttgcta cgattttttc tgtttctttc tgagcaagat ctgctgtcat ttttcctgaa
    46981 aatgctgcgg ataaaataag cagtaagaaa aaaccgaata accaaaagag aagaaacggt
    47041 ctttttttcc tcatgccttc ctccactcct atttaatttt gactattcta ttatatacaa
    47101 aaaatgaaat agaaagacaa aaaagttgaa aaagatcgta aataattgaa cttctttttc
    47161 ctgaagcggt aagaacggga attttcatac attctctctt ggctattctt taagcttcaa
    47221 atccatttct gtctgataca tctttgaacc aatgacccga tttcttgatc gttctttttc
    47281 catcttgtgc taaatctact gaaataaaac cgtaacgatt cttataggca ttggtccacg
    47341 accagttatc catgcatgtc cacatatgat aacctaaaca attggcacct tcttgaatgg
    47401 cttgatggac atatttcaga tgagattgaa caaattcgat ccgataatca tcgtggatca
    47461 taccatcttc cttgataaat cgtgcttcat tttctacacc catgccgttt tcagaaataa
    47521 aacagtcgat attcccataa tgatctcgta cattgatcaa aatatcatag attccttttt
    47581 catagatctc ccatccgcga taaggattca ttttacgacc aggcatttca taattgtcga
    47641 agtaatcctc aggcatagga ccgtttttag tatcgattgg tgtttctttt gcttttattc
    47701 ggcgtggttg gtaataatta attcccaaaa gatctactgt gttttcttta atgatcgtca
    47761 aatcatctgg atgaatctcc ggcattagat cgagttcttt tattctctca atcaattcac
    47821 tgggaaactc gccttttaca gctggatcaa ggaatgaacg attgaagaac gcatctgcta
    47881 ttcgtgctgc tttaacatct tcgggatttg attcgtctct tggataactc ggtgtcaaat
    47941 ttaagatgat tccgatcaca ccgctttgat ccatttcatg gtaagctttg atggctaatg
    48001 ctgaagccaa cgcttcatga taaccaactt ggattgcctt tttcatatct gattcttccg
    48061 gataatgcca tcgatacaaa tagcctgcct cgactggaac gattggttca ttatgggtaa
    48121 accatttttt tactttcgag ccaaacaatt gaaaacaact ttttgcgtat ttttcatagg
    48181 cttcaaccgt ttctcggttc aaccatccgc ctttttcttg cagatacatc ggcatatcga
    48241 aatgatacag attaacaaag ggttcgatac cgttttcaat caactcatca aaatagtgat
    48301 gatagaaacg aacggcttct tcgttgattg ttccagattc atcaggtatc aaacggctcc
    48361 attggataga ggttcgaaat gaattatgcc cagtctgttt catcaactgt acatcctctt
    48421 gatacttcgt ataaacttgc gaggtttttt caggtcctac ttgatcaaag aacttttgcg
    48481 gttcgttttt gtaccaataa tcaaaaatat tctctccttt tccatcgcct gaaattcttc
    48541 cttctgtttg tggaccactt gctgcagagc cccaccaaaa attttttgga aattgatatc
    48601 ttttcatcaa agcacctctt tttctttttt agttgattcc atgtatgata ttttccatca
    48661 caatcacttt ttcattttct tcctcttctt ttttgattcg tttcaacagc tgttctgctg
    48721 ctcgttcccc gattttatct gttggtggcg tgatcgtgat ttttggaagg atactcagat
    48781 cgtttttatc taaataatca tagtgactga acgcaatatc cttcccgatt ctgacacctt
    48841 cttgattgaa acattcgatg gcaccaatcg acatgttgta attgctgaca aaaatagcag
    48901 ttgccccttt ttctaataat gcttttgccc cgtaatagcc gctttttcta gaatagtctc
    48961 ctgtaaagat ctctaaatgg atagacggat ccttttgtac cgcttctttg gctccagcta
    49021 aacgttctct tgctacgtaa tctgtttgag cggcagcaat aaatcctatt gttttatgtc
    49081 cttgtccgcc atgatgagca atcacttttg ctacactttc tcgatcattc actactattg
    49141 tatcaatacc tgctggccca cccggacaat tcaatgcgac aactgggata ctcatttttg
    49201 tcagcatttc aaatccagac cactcttctt ctgataagaa gacgatcaat ccatcgatcg
    49261 catgcttcat caggtattcg attttttgtt cgattttttc tggctgatcg gcaaacccgc
    49321 ttaataataa gctataccca ttttcttcag ctactctttc gatccctgaa acaacctccg
    49381 aaccaaatcg gttagatatc ccgtttatca acagtccaat agagaaactt cgattattct
    49441 tcaatccttt agcaatgatg ttttctttat agtccaacgc tgaaatcgct tctgatattt
    49501 tttccatatt cttttccttc agctgctgac cgtttaaata gcgggaaact gttcccactg
    49561 aaacaccagc catcttagcc acgtcacgga tcgatgccat acattttcac tcctgccagt
    49621 tacatttgat ttctttttct tgttgttcga ccatgatgac cgctttcttt gcataatgcc
    49681 gcttgtcatc aatatccata taactaaagt cttcagaagt gagttcaaaa acaagcagtc
    49741 tttcctcttg agaccgcaat tcatctttac gaaaagcttt caataatttc gttctttgga
    49801 tgacaacgcc gtcaattaat cggacataga caagagtact tgttttacct gaaacacttc
    49861 ctgtattttt aactttaatt gatattatta ctttgtcaat atccgttttg acttccatct
    49921 gactgtattc aaatgttgta tagcttagac catgtccaaa aggataaaat ggacttccgc
    49981 ttaaatcata ataattctct tgtttagaag cagcacgctg atagtaataa acaggtagtt
    50041 gccgactatt tctaggataa gacaaactca atcgtccgct tgggttattt cttccagaaa
    50101 tcatctgcgc aatggctttt ccaccctcct gtccaggaaa ccatgctgtt gcaatggcat
    50161 tcgtaaatga ctcgattttc atcaaatcgt agggtcttcc ttgcacaagt atcgtgatga
    50221 ttggcttgcc tagttgatac aattgttcaa tcagctgttc ttggtatcca cctaaagaaa
    50281 gagaagcaac atctacattt tctccagaat ccatgttgat ccctttcgat gaaactgccc
    50341 cgttacgaag aaattccata tcaaagttcc gcgcacttga tccacctaga actgcaatga
    50401 tcacatctga gttttgagca agggtcaccg cttctaataa tttttcttcc tgatcctcct
    50461 gatttcttac ttcacatcct tgtgcataag aaacctttga ttggggaaac tctgttttta
    50521 tctcttgtac tatctttttt ttcaaacggt cttctgtttg tggtgcagag tagtctccca
    50581 acaagtggta aacatcgtga gcatttggac ctataacagc aatgtttttt acttgttgat
    50641 ccaacggcaa tataccgtca ttttttagta aagtgatact ttcttttgct gtctctaaat
    50701 ttaagtgctc tgattcctcc atgagatgat cccaatactc ctttggatcg tttgcaaatg
    50761 gctgttcaaa taaacctaat aaaaatttga cagataaaac tcggcgaact gcttgatcta
    50821 gtagcttttc atctacgact tgattatcaa tggcttctgc aatcttggta taggtctcat
    50881 cccacaaact gagatcgatt cctgccgcta atgcatatgc tgcagcagtc tttttatctg
    50941 taaaaacatc ggataaacga tccaaagcca caccgtctgc cataacgatt ccttgataac
    51001 cgatctcttt tcttaatgtt tgctcaaaaa gcgctcgatt gatatgacaa ggtatcccgt
    51061 cgatgtcatt ataagcagcc atgacaccta ctgcatttct tgtactcttc aataatggtc
    51121 cgtaaatatc ggagaactct ctgcgtccaa tcgttactgt gcctgaatta tgaccgccta
    51181 aagcttctcc ttgtgcgata cagtgcttta acacaacacc gatctgatcc ttcttcttat
    51241 tgatttcctc tactgtctga tcaagaaaag aaactttttc tgaaataatc tctccttgga
    51301 accctcgaac gaccgcttca gaaaaacgag ctgagagaat cggatcttcc ccaaagcact
    51361 cttctgtcct tccccaacga ggatcttttg ccagatccaa agtcgacacc agcgctaaat
    51421 gaacaccttt agcagccagt tcttttgcca tcaatcgact cgattgttct aataatgcta
    51481 cattgaaagt atttccacgg ccgatattcg ttggataaga aatcccatcc aagccttgat
    51541 gaccatgtgg acattcttct acaagcaaaa ctggaatttt ccatcgcgaa tgctgtagta
    51601 catattcttg gactaggtta gccaccttcc agctttcttt tgcaggaatc ccattcttat
    51661 aatctgtttt agaccacgga tcagagcgaa acagaccgta taaggcaccc agaccgcctc
    51721 cccatttaac atgttctttt aacttttctg ttaattcgat cttgtctccg tttttttcat
    51781 aacattccca accatacaaa tgctggttta cctgtcctac tttttcagca atattcatcc
    51841 tcgaaagtaa atcttctact ctttggcgga tggttaactt tgggttttga tacgggtaat
    51901 cgtacacctt aatatcctcc ttctgccaat tttgccttat tagcggctaa tacaaatggc
    51961 aggaataaaa atacatctag aatgataagt attactgtta agactgctgc tcgtatatcg
    52021 cctcctgtag caataaaaga ttgtataacc ggtggagtga tccaaggtgc agcagcaact
    52081 gttttactga tgattccaag acttgtggct gcgtaggcaa taaccaagtt aatagatggt
    52141 ataatagcgc atggaatcat atatattgga ttcatgacta ctggtaatcc aaacataatt
    52201 ggttccgtaa tattaaataa acaagtagtc agaccaaatt tagcgattga tcgctgttct
    52261 tgacgtttag aagcccacaa gatagcaata actaacggta aaatacaccc gccacctccg
    52321 atcaatccaa atgcattggt aaagggctcc gtaataatat taggaatttc ttttcctact
    52381 gaaaaagcat ccatgttttc ctgaatagac tgtaacaaag gtgcttgccg tataggagcc
    52441 aaaatactcg aaccatgcat accaaacacc cataagaaat cagagaaaaa ctgtaaaaat
    52501 aagatgcctg ggacggataa aacaaaagct ttcaatggtg tctgaatcgc taattcaatt
    52561 aattgcggaa cattcaaacc agtcgtatgg tttaaagtaa aaacgacaac tgaaaaaaat
    52621 aaaatgacaa ctatttctgg tactaaagaa ttaaatgacg cagctactgc cggtggaact
    52681 ccttctggca tcctgatttt aaaactcttc attttcgcta atttgataaa taattctgta
    52741 ctgacaaggg cagcaatcat cgccgttgct aagccagacg aagaagtcag actttgtaag
    52801 aaaacccctg tagcttctgc tgtatttccg tccatcaaag tgactgtact tgataaagac
    52861 atcaaaataa acattacggc tgtacttaga attcctgcat gaacaggact aaatgatgga
    52921 tcatcaatca cgcctgtttg gatatagtgg gttgccaaat tccagccgat attgtaacac
    52981 acgataatag ctaaaatact catcgtacca ttattcacga ttgaagcaag ttcagccaat
    53041 ggacttagat caagtattct ttggactgtt gcatttgaaa aaatcagagc attcagcaaa
    53101 accatgacag aagcagccat tactaacggc atagtaacga taaaactgtc tcgaatggcc
    53161 agcaagtgtc gctggctact catcttagat acgattggta gcattttttc agatagtctg
    53221 tctacgactt tcattatttc atctcctaga ttctaaaatt gaaccggttc atttttaata
    53281 tagcactttt tttaaagatt gcaacacatt tttttaaaac gctttctaaa ggtggtggat
    53341 tttttatgac ttttgaaaaa ttattctatt taatggtatt ttgtgaattt tttctcttcc
    53401 atccgtttga agttttactt tccaatggta taatttcttt atcctatcaa atgggggaag
    53461 tattttgaag aaattaatcg tactatttat tttgtctgcg ggacttttga cattgactgg
    53521 ctgtgatttg tttggcggct ctgaccaatc ctcggacgaa acaaccgatt cttctgtctt
    53581 aaaagcggaa aaagcagata tcaccaacag tgtatatacg ctaaaagtac gtaaagatat
    53641 ggatttatcc gcgggacagt tgacatttac agataaaaaa atggaatgga cacgcacgta
    53701 tcagacacaa ggagctgatt cttctgaatc atctgaagaa aaaacagtgc tgacagatat
    53761 caagattact accaaagatg acgaatatat tatttcagga aaagaaaata aaaaagaagc
    53821 aacagtcaca tttaccaaaa tcggtaatta ccgtttgaag gataaggatg gcaacatcta
    53881 cagtctatag tgccatctgt tggatcttct tttgtttctc cccttctttt ctgctatagt
    53941 aaagaagcaa gatgactgta aaggagacga aatggatgat cgatagtcct gattattgta
    54001 catgtaataa caacaaaaat atccatacag aatttaatga tcaaggaagt ttgagtatat
    54061 gcgacaattg ccacaaacta gtcaaagatt cttttaaatc gtttgaatct tatcctgtca
    54121 aaaattatgt aaaaagcaaa agaagataag caaaatcaag caaaactcac tatggctgat
    54181 ttttatgaac ttcaaactca gcttcttcct ttgatcaagc ttcaaaaact ggaatcagta
    54241 aaacaaacag tggatcattt cagttcacaa atcaaagagt aacgttttat ttatttcaca
    54301 tctttgaata taaaataacc aaacgataga aagacttatt ctacatcacg tagtaatttt
    54361 tctttcccgt cgtttggttt tttactttta gcctaattct tgacctttcg catgctgatt
    54421 atctgtaaaa tattttccag caggctttcc ttccaattca gctaattttt cttggatctg
    54481 acgaatcacc ttttttgcat cttctggatt ttctacaaaa tcgaatgtat ccccatccac
    54541 ttgtatttta ggacatacat caaaatcctc ataccattga ttatagcggg tgttcagtgt
    54601 ttcgtaatag gtgtataacg tttcatcgct ttctagctgt tcatagtcac gcccgcgttt
    54661 tttgatacgt tctagcatcg tgtccaaaga aactttgata tggaccatca aatcaggacg
    54721 tttttttgga gcaacatcat ctaactcgtt tagcatcgta tctaataagc tatcgtactg
    54781 ctgtacttcg atatccgtta cccgacctaa atcagcattt agatggaaca acagactatc
    54841 ttcgtagatg gagcggtcta gcacattgtc atcgttcact aaagcatttt tcattgctaa
    54901 aaatcgctta ttcaaaaaga agatttgcaa taaaaatgcg tactgctcag gatttgaata
    54961 aaaaagcggc aatacttcgt tatcttcgac attttcataa aatggtttag aatcaatctc
    55021 ttttgcaagc atatccgtca aactgctctt acctgcacca atcgttcctg aaatcaaaat
    55081 cactgacata caacccttcc tcttctataa gtactcgcct acgtaagttt caacaggcaa
    55141 tttttcaaaa tcatattcac gtacagcttg gtctgatttt aaatacgtgt gaagactttg
    55201 tgtaatcatc aagttcgtag gtaccgtctc ttcttgtacg atgatcatcg gttttttcaa
    55261 catagttgcc acacctaatt cgtaagccgt tcctggatcg attgtttgat gctcaaagtc
    55321 aaggatagcc aaaacaaatt ctgcatttgt caactcttcc atatcttttt cgtatacttc
    55381 ctgcgcccat ccagcgctga atagttcata attgctttct tggtgatgac gtggagagta
    55441 gaaacttgtg attgttttgt tttcctccaa cgctttttcg atacgactta ctcgatcgat
    55501 ttgctcctct gaaaaaaatg gtcctgctaa atatatttga ctcattattt tgcctccttc
    55561 aataataact gatttttaat agatactgct cgtaattcat tgaccagttc caatggattg
    55621 ttggaaaaga tcacttgatc caaccatttt ttatccataa aatcctcttt tgcaaaagtt
    55681 tctaataatt gttgcaagcc gctgtaataa ccgccaatat tcaatattcc gattggctta
    55741 tcaggtgtca ggccgatcgc tatccaagaa agaagttgcg aaaattcctc caatgtacct
    55801 agacctccag ggaaaacgag gacagcatct cctttttcaa ataataatac ctttctttca
    55861 tcaattgtct ctgtaggaat aaatgtagta acatctttcc caggcagctc agtttcaaaa
    55921 aggccatttg gatagatacc tgtgactttt ccgttatttt ccagtactgt tttactaatc
    55981 actcccataa tcccggagta tgaaccaccg taaaccagtt cgatttcttc ttgtgccata
    56041 tattttccta acgtttgcgc aataaatttg taactttctt tattgccaaa acgggaaccg
    56101 caaaaaacag taagtttcat aaaaaaacac tccctaactc aaaagtctat cttcattcta
    56161 cacgtgtttt tttaaaataa aagactagat tttgtagttt tttagtggta tatagatcaa
    56221 acaccactat atatagtatt tgcaaaaaaa taatttttca gctattttac agtaaccaaa
    56281 aaaataaata actagtaaat ataaaattga aacgacttac ttatagaaga aattgggatt
    56341 tcgtcctgca tgtcaataac acgcattatt taaatagtca cacacttgtt tctcctatta
    56401 atgaaaaatc cattttattc tcgtgttatg tttcaccaac gcctgatgtt tctctaaata
    56461 aaaaagccga cgaaccaaat aatcgaaatt tgatcatttt ggttcatcag cttgttccca
    56521 aacctttgta ttaacgataa atagctggat tcgtaaaact atcacttgga tcatcactgc
    56581 tggaagaaaa ttcggtgata accgcgcctg ttgcacctgc ctggaaccag tgtttaatat
    56641 tttgttcgat cgtgtactga tctccagcgt gaagtatgat ttcttttttt gccgtatagt
    56701 aacgttcttg cccctttgga ataatacagg aaagtttttt tgtttctttt ccttcgacat
    56761 aaagatatac taagcctttt cgacaacgaa aggtttcctt ttttccttca ttctcatctc
    56821 gaggtgggtg ccgatgctct ggacaagttt gataaggcaa taataccagt tctttcccac
    56881 agtatcgctc attattttca taaacaaata aattcaatcc ttcagtctcg attgctccta
    56941 atccaaaatc tgtatattcg attctttcta attctgtttc tttcaatact actcctgaat
    57001 ctaggaacat ttgttgtact cttttcttgt aatccattct gttcctccta actgattgtc
    57061 aagtcatccc aagatatctc ttcttcctct ttttcttctt ggatgacttc tgcatttggg
    57121 tcaactggtt ttttcaataa taaaatagtt attcccgtag caaaagaacc agctaacaat
    57181 gcaccaatga atgcccatgg tctggtcatc gtagcgactg caaacattcc cgatgccgga
    57241 atacggctat ctgcgcccca aaacatgctc aatccaccgc cgactgcccc accgactccc
    57301 gttgctatga cattgcgtgc caagtcgttc aatacgatag ggatacatcc ctctgtgatc
    57361 tgacagatcc ccataggaaa agcggtcttc aatgtatcga tttcttgacg attgaatata
    57421 tttttacgaa tgattttccc taagaaataa gcgatcgtca agccgaatgg ggttaccatc
    57481 gaagctaaaa tcaatacagt gattggttca tagattcctt ctgaaaatcg tgccacaccc
    57541 ccttatccag aataaatggt aatccagaaa cagccgggta tctgcggtaa taagcctaac
    57601 caaacgaaaa atatgagaag ctaatatgag aagctatttt tgtttggctg ttcttattac
    57661 ttgatgcagg gcagcttttt cacaacctct ttttttattt atgtttcata atcaatatgg
    57721 taaggatgca gctccgtatg gtagaccgcc tccgaatatt caatcatttc cccaatttgc
    57781 gtagacgatt tccgtatccg tttgatgccg atctgtgcgt ctgttttcag aatttcttgt
    57841 tcttcttctg ttaaaaatac tgccgagaaa tcatcagaaa atgagtcaat agtatatcct
    57901 tttttcgtca gcaaacgata caaagattct ttgccaaatt cttctctgct gaattgcatc
    57961 atttcttgtg gaagataatg aacaaaataa atataaggct gattgttcaa ataatataag
    58021 cgcttaaaac aaacagcttg ctcaccgaaa aaggaaaaca aaggatcttc cttctttata
    58081 tggaccaatt gcaaatccag cgtttctttc cggacttcat atcgggagtt ctccaagatt
    58141 tgcgtaaagc ttgccgcttt ggataatcga ttgtaaggac gattgcttag aaccgtcgtc
    58201 ccacgaccac ttttctttgc gacatattca tctgatgcca gcagttcgat tgcctttcgg
    58261 acagtgattt tgctgacttg aaacagttct tctaattcac tttctgtcgg taacatcgaa
    58321 ccgactggat attttcctga gaaaatatct tctttaattt tatctgcgac ttccaagtat
    58381 agtacacgtg tccttttcat aacgtcaccc tttgctttac tattactaaa agaatagcac
    58441 ataaaaaata aaaaaagaac acaaatcaca tgtgagaagt gtgttctttt tcattcagct
    58501 gctttaatta caagtttacg ccatttgagg caattacttc tttataccaa tcaaaagatt
    58561 ttttctttga tcgattcagt gtcccatttc cttcatcgtc tagatccaca tagataaagc
    58621 cgtaacgttt actcatttct cctgttgatg cactgacaag atccatacat ccccaaggcg
    58681 tatagcccat caaatctaca ccgtctgcta ctgcattagc catcgcttcg atatgacgac
    58741 gtaaataatc gatccgataa tcatcaataa tcgtgccatc ctcttccata tgatcaatgg
    58801 ctccaagacc gttttcaaca atgaataatg gtttctggta acgattgtat aattcattca
    58861 atgcgattct cagcccttct ggatcgatct gccagcccca ttcacttgcc tcaagaaacg
    58921 gattttttac tcctcccagc aagttccctt ctgaagttgc tgcttgtgga tcagtctcat
    58981 tgattgccgt agacatataa taactaaatc cgatgtagtc cactggatat tcttttagca
    59041 actggaagtc tttttccgtt tgctctaatt tttccggatc gactccatat tttttatggg
    59101 tacgcgctgt gtatgcaggg tattctccac gtacttggac atccgtgcag aaaaaattga
    59161 attcttggtt ttggatcatt gttgctgcct gattgactgg attggcatcg atgctgtaag
    59221 ttgttgcata gataatcatg cagccgattt ggatatccga acgcaattcg tggccgattt
    59281 ttacggcttt gctacttgca acgaattgat tatgccatgc ttggaaaata ttttggtact
    59341 ctcctgctcc attgcttttt actaatcctt gacttaaagc tggaaagtga aaggccgaat
    59401 tgatttcatt gaaagtcatc caatatttga ctttactgct gtatctctct aatactgtct
    59461 gagcaaatcg ttcatagaag tcgatcagtt ttcgattttt ccagccaccg tattcttttg
    59521 ccaaatgaag aggcatttcg taatgagaaa tcgtgatcac aggttcaata tcatatttta
    59581 agcactcatc aatcagctga tcataaaatt ctaatcccgc ttcgttagga gtaccttcgt
    59641 ctccatttgg aaaaattcgt gtccaggcaa tcgaaaaacg ataacatttg aatcccattt
    59701 ccgcaaacaa ggcaagatct tctttgaaac gatcgtaatg atcaattcct cgatgattcg
    59761 ggtaaatata tttttcttga tcaatcgtcc agtcaaattc ttcactgccg ataatcgcaa
    59821 agcgctgctt acctccaggc atagcatctg caacggatag cccttttccg tctcgatcat
    59881 aagccccttc taattgattg gcagcagttg ctccacccca taaaaatttt tctgaaaaac
    59941 gtgattgcat cctgatttcc tccttatttt acaattgcca gaacatcttc gtttgcaata
    60001 atctcttttt ggtctaattc aagtatatcg gcaaatgcat cagtattcgt aatgacaact
    60061 ggagtgatta cgctatagcc agctgctcta attgcttcga tgtcaaaacg aatcaataaa
    60121 tctcctgctt tgacgagatc cccttgtttg actaagatct caaagccatt cccatttaat
    60181 tctactgtat ccatcccgat atgaatcaaa atctccgctc cgtcagtcgt tgtgatccct
    60241 acagcatgtc cagttggaaa aatagtcgtg atctctccgt ctgcaggtgc aactagttct
    60301 cctacttctg gatcaatagc gatacctttt cccatcacgc cagaagaaaa aacttggtct
    60361 ggtacttcac tcaatggcag tactttaccc gttaaaggac tttttaaggt atgccgtgct
    60421 gaagtaatcg gttctcctgc attcgcatgt ttgttttcta attgattgtc ttgtgtttgt
    60481 tcgtcaaatc ctaaaatcaa tgtacctacg aatgcaagaa caaaagcaat tcctgtagct
    60541 attactgcta ctgtcacatt tgactctaca ccatccaccg tattaatgaa agtcggaatc
    60601 gtcaataagc tgaccaaact gctggagaaa gcttttactc cggaaaatcc gataatagct
    60661 ccaccaatac ctccagaaat acaagcggca atgaatggtt tcttcaaagg taatgtcaca
    60721 ccatacacag ttggttcagt gattccaaat aacgaagtca ctgtagaaga aatccctaat
    60781 gctcgaagtt tcgtatcttt tgttcgcaat gctacagcta aagcagcccc accttgtgcc
    60841 aagatggctg gaagaagcat tggcatcaat acatcaaacc catattgttc gatattcaaa
    60901 aacatgatcg gtacgaatcc ccaatgcatg ccgaacatca cgaatacttg ccataaactt
    60961 cccatgatta gacctgctac gattggacta aatccataaa tcgagttgaa gagaccgcct
    61021 aaagcattac cggcaaccgt cccgattggt cctaaagcaa gaaatgttaa tggaaccatc
    61081 actactaaaa cgacaagcgg aactaaaatc atttgcagaa attgcggaat cacttttttt
    61141 acgaatggct ctaatttact ttgaacccat acggctagaa tgattggtat gacagaagaa
    61201 gtataaccag atggtgaaag aatcactggg atcccaaaga aactgatagc ttctcccgca
    61261 ccagcaactg ctgtgatagc tggatacaca agtgccatag caattgctac tgctaaaaac
    61321 tggttggctt tgaacttttt agctgctgta aaagcaagca tgacaggtaa gaaggtaaag
    61381 acaccatctg ctgcagcaaa caatatttga taagtaccgc tatcagctgt cagccagccc
    61441 atcaccgttg ccaatgataa gaatcctttc aataccccgg cggctgccat cgcccctaga
    61501 aaaggtgtaa atatactaga gataatatcg atcagacgat tcaaaatatt tttggactct
    61561 tcttttccat cctccgaaac tgcaagacct tcctcatcca cgatcgattg ataaacatct
    61621 gctacattac tgccaatcac tacttgatac tgaccgccac tttgtacgac ttggatcacg
    61681 tccgggtctt cttttaactt ttgcgtatct gctaaagatt catcttttaa tctgaatcgc
    61741 aaccgcgtag cacaatgtac taaacttttg acattctttt gtcctcctac cgcttcccat
    61801 acacgacgac caactgcttg attatccatt gttttttcct tctttctttt ttatttaaaa
    61861 aataaaaacc cagattgaaa aaaacagtca caaacattcg tgaacctatt ttttcaatcc
    61921 aggttttgcc tgccatacag taacaatcct tatgattgga tgcttttttt ctcacttact
    61981 actctttgaa tatgaatcgt taaataaatc tgctctgcga tccccatttc ctgtttttgc
    62041 gtttgtcgaa gatactcatt gatccgttcg acacaatgaa acgcttttgg atactttcct
    62101 tgaactaatg cgtagagaaa ttcatcttgc tcgtcagatc cttccccttt gagataacgc
    62161 tgggcaaaaa actgcagatg agtaatgata cgctgataat tcaaagattc ttcatcgaac
    62221 acgatgccga agaatttact gatgatactc agtatatctc ttaccattgt ggtagcggtc
    62281 atcgtcactt gcatgtcttc gtttccttgt tggctattca caaaatgaaa ggcaataaaa
    62341 ccggcttcat cctcagaaag ttccacaccc gtcttttgtt tcacgaacaa aagagcctct
    62401 ttcgctgctt gatattcttt cggataaaat ttttttgttt caaataataa tggattaggc
    62461 agttggatat tttcttttgc tcgtgtgatc gcataatgaa gatgatcggt caatgtcaga
    62521 taaatattag gcgaaaattc aacttttagt ttatcttcag ccagttcgac tacttgtttg
    62581 acccattcga tctcctctgc cggaatatca gccatcagct gctggaattg gtttttagca
    62641 aaagaatctc ttaaaacaaa ttctttttgg accgcttctg gcgaaatctc gtccccaatc
    62701 tttttttgaa aagctagtcc ttttcccata caaacgactt cttccccgaa tccatttttc
    62761 gaaatgacga cattgttatt caaaatttta tcaattttca ttttgcttcg ccctctcaag
    62821 aaaaataaaa aaacctagaa ttcgcctact gtttcttagc tgaattctgg ttttgcctgc
    62881 cgtacagtaa caatccattt cttattcaaa atcatcatac catcgaattt ttatttatgc
    62941 aagccttttc atataaaaaa taaaaaaact ggaacacaag tctatctaga aaacaaccaa
    63001 atcagagatg cacctctttg cgtttctctt tttttcagca aattggtttt tccttatcga
    63061 ttcatatcct ttggctgttt ttaatctgta acgacttatg tcccagtatc ctaaaatgtt
    63121 tttgtctcta gattgaaagc agatctcata tcatctgtcg catcttttat cgatgtaaca
    63181 ctttccagtt ctccttgtac aactagtcgc gtgacggcgt ccatgtcgcc acaagtaatt
    63241 agtgtgacaa tttcttttcc ctcgacttca ttcagtaatt gggtatctgt aggagcaact
    63301 ttttctttaa aaaatgtttt gtatgtatac acattttcta aatctgtcaa atagatcttg
    63361 gcacccatct ctaaattttc tagcggtgta aacagcaatt cagggttcgt tgcacgatgg
    63421 cttgctaagg cataatttcc ttttcccatt tcttgatctg gagataatgt tcctgctccg
    63481 taaagtagtg acgtattatc caagcctttg aaaaccggta aattgattga aactgaaggg
    63541 actgcgatac tagcaatcac tggtagatct gttcctctca tttgcgacct catgacccct
    63601 tctgtcgtca ttggttctac cgcatcaaag tcaaaactga catctttatc gttattcttt
    63661 ttcagatctt cttttgttac attcgctata gcatatttat ctcctgtctc tctgacaaaa
    63721 taatccttga tctgttcatt gaaaatcaaa gctagtccta ctaataacaa gaggaacaag
    63781 aggatattga ctagccatct ttttgctttt cctttttttt tcattttctc tccctccaga
    63841 ctcattttac tgtttttctt ttgctctttc caacagccga atgacacatt tctacaactc
    63901 tgtgtatttt tttcgtacac ctgcgctttt ttctacagga tatttcaaat tatttttgat
    63961 caatttttct tcaatgatct catctagatc caggtgaagg ttgtctgcca tcatgtatga
    64021 atagatcaat acatctgcca gttcttcttt gattcgttct aattgtgttt cttttacttc
    64081 ttccggcgtt ttccattgaa aaagttctaa caattcagat gcttccaaag aaatagagat
    64141 agccaaatct ttttcgttat gaaattgtcg ccaattacgt tcatctctaa actggttgac
    64201 tttttccata ctattcattt gcttgccttc ttccaatcac ttcttacttg attaatcata
    64261 ccattttttt acataaaaaa tagatgatgc ttttaaaaaa actcctgttt tcttttattt
    64321 aaaataaatg tcatagaaaa tcaataattt aggtagccac catgtgtatg cagtagcgtg
    64381 tcttcttccc ttgaggcttt ttgtttttcg atacgttctt cttcccaaac agacaacgct
    64441 actagaatct ctcttaatga ttttccttct tcggtgagcg agtactctac tttaggcggg
    64501 acttggttat aggcttttcg atggatactt tcatcttgtt ctaactctct taactgctgg
    64561 gtcagcattt tttttgtgat cgtagggatt tgcttgcttc tccacgccta aaataagcga
    64621 tattgctctt ccctttatct ggtgtaagca aagcagttag cattttgacg atcgtgggtt
    64681 ccgcttttca cttcttatct acttagtatc atgaagtata ctttcgtact aatactaagt
    64741 agcggaagtg tcaactactt tgtttaacta catagcaaaa gaaacagaag ggtgacaacg
    64801 gtcatcacgc ttctgtttct tttgaatatg ctgtgttgtt gtctctatct ccgtgtagac
    64861 tttcctcaaa cttatccttt gacagtaagt cagtaggctg atttgctctt tctagatgcc
    64921 agatacattt ctacaaaaaa atagtgattc tgttttgaaa attcgatttt tagttctgca
    64981 ttttcgtaaa catccaacat ttttttcgca ctgtatagac ctaacccgcc atttttttta
    65041 gaggtttttc ctcttttgac catctccgat agcgtcaaat tcgttggatt tgccttgttc
    65101 gctatactaa aacgtgatcc ctctttttct tccagcagat tgatcgtaat cggactctga
    65161 tcggctacac gatgatcgaa tgcgttagaa ataaaaatag ataaacagcg aataaaaaca
    65221 accagatcaa tcgggattgt tttcacttcg acaggaataa ctacttggat cgggattttc
    65281 tccgattctg ctttttctag aaattttaaa aagacacccc tgacagctgg ggatacaata
    65341 tttgttagct gttgtatttg cggtttttca aaaatagctg cagaatgatc ttctaatatt
    65401 tctaaaaatt ccaac
//
LOCUS       DACDIH010000007        63297 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDIH010000007 DACDIH010000000
VERSION     DACDIH010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.007"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(199..1092)
                     /locus_tag="IV281_000453"
     CDS             complement(199..1092)
                     /locus_tag="IV281_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP8779494.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(1274..2200)
                     /locus_tag="IV281_000454"
     CDS             complement(1274..2200)
                     /locus_tag="IV281_000454"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP8779495.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(2285..3046)
                     /gene="pflA"
                     /locus_tag="IV281_000455"
     CDS             complement(2285..3046)
                     /gene="pflA"
                     /locus_tag="IV281_000455"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP8779496.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(3290..5521)
                     /gene="pflB"
                     /locus_tag="IV281_000456"
     CDS             complement(3290..5521)
                     /gene="pflB"
                     /locus_tag="IV281_000456"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP8779497.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(5798..8248)
                     /gene="parC"
                     /locus_tag="IV281_000457"
     CDS             complement(5798..8248)
                     /gene="parC"
                     /locus_tag="IV281_000457"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP8779498.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(8260..10305)
                     /gene="parE"
                     /locus_tag="IV281_000458"
     CDS             complement(8260..10305)
                     /gene="parE"
                     /locus_tag="IV281_000458"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP8779499.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(10471..10905)
                     /locus_tag="IV281_000459"
     CDS             complement(10471..10905)
                     /locus_tag="IV281_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP8779500.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11032..11670
                     /gene="plsY"
                     /locus_tag="IV281_000460"
     CDS             11032..11670
                     /gene="plsY"
                     /locus_tag="IV281_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP8779501.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(11802..12677)
                     /locus_tag="IV281_000461"
     CDS             complement(11802..12677)
                     /locus_tag="IV281_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP8779502.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(12759..13550)
                     /gene="codY"
                     /locus_tag="IV281_000462"
     CDS             complement(12759..13550)
                     /gene="codY"
                     /locus_tag="IV281_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP8779503.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(13568..14968)
                     /gene="hslU"
                     /locus_tag="IV281_000463"
     CDS             complement(13568..14968)
                     /gene="hslU"
                     /locus_tag="IV281_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP8779504.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(14982..15530)
                     /gene="hslV"
                     /locus_tag="IV281_000464"
     CDS             complement(14982..15530)
                     /gene="hslV"
                     /locus_tag="IV281_000464"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP8779505.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(15567..16472)
                     /gene="xerC"
                     /locus_tag="IV281_000465"
     CDS             complement(15567..16472)
                     /gene="xerC"
                     /locus_tag="IV281_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP8779506.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(16670..18046)
                     /gene="trmFO"
                     /locus_tag="IV281_000466"
     CDS             complement(16670..18046)
                     /gene="trmFO"
                     /locus_tag="IV281_000466"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP8779507.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18015..20093)
                     /gene="topA"
                     /locus_tag="IV281_000467"
     CDS             complement(18015..20093)
                     /gene="topA"
                     /locus_tag="IV281_000467"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP8779508.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHGHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(20215..21072)
                     /gene="dprA"
                     /locus_tag="IV281_000468"
     CDS             complement(20215..21072)
                     /gene="dprA"
                     /locus_tag="IV281_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP8779509.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21128..21895)
                     /locus_tag="IV281_000469"
     CDS             complement(21128..21895)
                     /locus_tag="IV281_000469"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP8779510.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(21888..22748)
                     /gene="ylqF"
                     /locus_tag="IV281_000470"
     CDS             complement(21888..22748)
                     /gene="ylqF"
                     /locus_tag="IV281_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP8779511.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            complement(22842..23801)
                     /locus_tag="IV281_000471"
     CDS             complement(22842..23801)
                     /locus_tag="IV281_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8779512.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            24073..24471
                     /locus_tag="IV281_000472"
     CDS             24073..24471
                     /locus_tag="IV281_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8779513.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24708..25250
                     /locus_tag="IV281_000473"
     CDS             24708..25250
                     /locus_tag="IV281_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8779514.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(25333..25884)
                     /gene="lepB"
                     /locus_tag="IV281_000474"
     CDS             complement(25333..25884)
                     /gene="lepB"
                     /locus_tag="IV281_000474"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8779515.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(26036..26263)
                     /locus_tag="IV281_000475"
     CDS             complement(26036..26263)
                     /locus_tag="IV281_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP8779516.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(26260..26778)
                     /gene="msrA"
                     /locus_tag="IV281_000476"
     CDS             complement(26260..26778)
                     /gene="msrA"
                     /locus_tag="IV281_000476"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8779517.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(26798..27511)
                     /locus_tag="IV281_000477"
     CDS             complement(26798..27511)
                     /locus_tag="IV281_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP8779518.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(27514..28410)
                     /locus_tag="IV281_000478"
     CDS             complement(27514..28410)
                     /locus_tag="IV281_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8779519.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(28568..29410)
                     /locus_tag="IV281_000479"
     CDS             complement(28568..29410)
                     /locus_tag="IV281_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8779520.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            29542..30195
                     /locus_tag="IV281_000480"
     CDS             29542..30195
                     /locus_tag="IV281_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP8779521.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(30258..30785)
                     /locus_tag="IV281_000481"
     CDS             complement(30258..30785)
                     /locus_tag="IV281_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8779522.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(30805..31752)
                     /locus_tag="IV281_000482"
     CDS             complement(30805..31752)
                     /locus_tag="IV281_000482"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8779523.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(31764..33650)
                     /locus_tag="IV281_000483"
     CDS             complement(31764..33650)
                     /locus_tag="IV281_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8779524.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(33810..35888)
                     /locus_tag="IV281_000484"
     CDS             complement(33810..35888)
                     /locus_tag="IV281_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8779525.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(35901..36176)
                     /locus_tag="IV281_000485"
     CDS             complement(35901..36176)
                     /locus_tag="IV281_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779526.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(36538..36993)
                     /locus_tag="IV281_000486"
     CDS             complement(36538..36993)
                     /locus_tag="IV281_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779527.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(37147..38355)
                     /locus_tag="IV281_000487"
     CDS             complement(37147..38355)
                     /locus_tag="IV281_000487"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP8779528.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(38467..38799)
                     /locus_tag="IV281_000488"
     CDS             complement(38467..38799)
                     /locus_tag="IV281_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8779529.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            38947..39822
                     /locus_tag="IV281_000489"
     CDS             38947..39822
                     /locus_tag="IV281_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8779530.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(39904..40485)
                     /locus_tag="IV281_000490"
     CDS             complement(39904..40485)
                     /locus_tag="IV281_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP8779531.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(40491..41750)
                     /locus_tag="IV281_000491"
     CDS             complement(40491..41750)
                     /locus_tag="IV281_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8779532.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(41752..42798)
                     /locus_tag="IV281_000492"
     CDS             complement(41752..42798)
                     /locus_tag="IV281_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779533.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFMWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(43033..43308)
                     /locus_tag="IV281_000493"
     CDS             complement(43033..43308)
                     /locus_tag="IV281_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP8779534.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(43551..44861)
                     /locus_tag="IV281_000494"
     CDS             complement(43551..44861)
                     /locus_tag="IV281_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP8779535.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(45043..46272)
                     /gene="rpsA"
                     /locus_tag="IV281_000495"
     CDS             complement(45043..46272)
                     /gene="rpsA"
                     /locus_tag="IV281_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP8779536.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(46407..47087)
                     /locus_tag="IV281_000496"
     CDS             complement(46407..47087)
                     /locus_tag="IV281_000496"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP8779537.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(47136..47771)
                     /locus_tag="IV281_000497"
     CDS             complement(47136..47771)
                     /locus_tag="IV281_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8779538.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(47837..49240)
                     /locus_tag="IV281_000498"
     CDS             complement(47837..49240)
                     /locus_tag="IV281_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP8779539.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(49227..50258)
                     /locus_tag="IV281_000499"
     CDS             complement(49227..50258)
                     /locus_tag="IV281_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779540.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            50289..50510
                     /locus_tag="IV281_000500"
     CDS             50289..50510
                     /locus_tag="IV281_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP8779541.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(50675..51412)
                     /locus_tag="IV281_000501"
     CDS             complement(50675..51412)
                     /locus_tag="IV281_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP8779542.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(51565..52905)
                     /locus_tag="IV281_000502"
     CDS             complement(51565..52905)
                     /locus_tag="IV281_000502"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP8779543.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(53142..54665)
                     /locus_tag="IV281_000503"
     CDS             complement(53142..54665)
                     /locus_tag="IV281_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP8779544.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(55044..55655)
                     /locus_tag="IV281_000504"
     CDS             complement(55044..55655)
                     /locus_tag="IV281_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8779545.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(55778..55903)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(55985..56701)
                     /locus_tag="IV281_000505"
     CDS             complement(55985..56701)
                     /locus_tag="IV281_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8779546.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(56707..57276)
                     /gene="scpB"
                     /locus_tag="IV281_000506"
     CDS             complement(56707..57276)
                     /gene="scpB"
                     /locus_tag="IV281_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP8779547.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(57260..58057)
                     /locus_tag="IV281_000507"
     CDS             complement(57260..58057)
                     /locus_tag="IV281_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP8779548.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(58027..58398)
                     /locus_tag="IV281_000508"
     CDS             complement(58027..58398)
                     /locus_tag="IV281_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8779549.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(58417..59304)
                     /gene="xerD"
                     /locus_tag="IV281_000509"
     CDS             complement(58417..59304)
                     /gene="xerD"
                     /locus_tag="IV281_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP8779550.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(59400..59873)
                     /locus_tag="IV281_000510"
     CDS             complement(59400..59873)
                     /locus_tag="IV281_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8779551.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(59978..60841)
                     /locus_tag="IV281_000511"
     CDS             complement(59978..60841)
                     /locus_tag="IV281_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8779552.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(61037..63136)
                     /locus_tag="IV281_000512"
     CDS             complement(61037..63136)
                     /locus_tag="IV281_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8779553.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
BASE COUNT        17137 a        13350 c        10813 g        21997 t
ORIGIN      
        1 aaaaatctaa cttttcttat cactacttcc tttgtcattc cacatttctc accatttttt
       61 tgcattcctt tgtttccaga ccatgtagtg aatctaatag gtggtagata ggcaaaatac
      121 tcaaataatt cttttaacca taaaggaaga gaaaggctcc tcctgcaaga gtcagtataa
      181 tttttgctta ctgctctctt aggttttact cactaaaact gttccatatt ctttgatata
      241 agaaaatgca tgcggtataa atttcttgct cagctcagaa aagatttgtc gtttaagaaa
      301 ggccatttcc tccattgtca atgaaggatc caatgttggt aattcaatct caaaattgtt
      361 tttttcgaca ataggtaatt ctaatgtgaa tgattcttct tcctttatag tattcgtcac
      421 aagtagagac tgatggaaaa tactaggccg gcgattttta tatttattct tacgtatcct
      481 gtcaacgata acttctattt gatcaaaaaa gtacgattca tttacgtctc caataagttc
      541 ggctaattct ttgaatgcct tcgttacagg aagctgattc ttctgtacaa aaaagtaatt
      601 agccaaagta agcgggaagt gttctataga ggaaagtgtg ataaaacggt acttctcatt
      661 tcctgcagtt gtcctcagca attctgtctc tcggctgttt cttactccat aaaaatacgc
      721 ttctttccgt tcatcttcga aacaatacgc ccaaataact tctcccatcg ctaatttttg
      781 ttcatcaaca gagagtctct tcaattgttc agctatggtt tcagcagccg aagtggcttg
      841 ttgcaagtaa tctttaggat caaatattgg aaagttcaaa tgatatcttc gttcttccct
      901 cttgattaat cctgcatcga tcaataaatc aagaaaatgg gaaaatttat tttgcgaaaa
      961 ttcttttttc aattctctta gaatcgtttc ttgttctcga tgttcatata gataatcggt
     1021 caatgaagaa aaaagcggat catcggtcag tttttgataa aagttttcct tattttttga
     1081 ataatagttc atatatgttc tcttttctat ccgttttatt ttacttaaaa ggcttttacg
     1141 taaaaaggag gaagaacaag agtcataatt gactgctatt ctcctccatt tcttcggcct
     1201 ttagtatgtt ctatgtttct ttttatatca atcgcttatc cattcatttt gaatgcttca
     1261 tactaccatt tgattagcta aatacagccg tcaattgagg tacgacttgt tttttacgtg
     1321 aaacgacacc tttaagcaaa gcacggttgt tggtcaaagt tgtttggaaa gcttcttcta
     1381 ctttttcttt tcctttacca gcaacaagta attctgaatc actgtctaag atgtttgtga
     1441 caactaatac gaacagatcg tagccatttg ctttcatttc atcttccatt gctgcttcta
     1501 gctctgcttg tcgatccatc acttcattaa ggtccactgt attgacttga gcgatacgaa
     1561 cgttggaact gcccattggg aaactttttg catccaaatc aagaagaacc gcagctgatt
     1621 tatcacttag atttgttcct gctttcaaca tttcaagtcc ataagtgttt agatcgactt
     1681 cagcgattgc tgcaagttct tttgcagctg cttcatcttc aggcgtacat gttggtgatt
     1741 tgaacaacaa tgtatctgaa atgatagctg agagcatgat tcctgcgatt tctttaggaa
     1801 ttgttacttg gttttcttta tatagtttta aaataatcgt acttgtacat ccaactggtt
     1861 ctgcacgata gtacaatgga ttagctgttt caaagtttgc aatacgatgg tggtctacta
     1921 cagctaaaat atttacttcg ctgatatctg aaatactttg ttggaattca ttatgatcca
     1981 caagcatgac atctgcattt tctgctttag ctgactctac tactcgtggt gcagatagct
     2041 gaaagtggtc taatgcgtat tgtgtttctt cattaggtgt acctagagca actgcttctg
     2101 tttctttacc taattcattt tgcaagtgtg aaaacgcaat ggctgccccg attgcatctg
     2161 tatctggatt ttggtgacca aaaactaaaa tttttgacat gtcattcgct cctttgtgat
     2221 ttctcctatt taataatagc aaaaaaacgg aaagaagcaa tacttatctt ctttccgatg
     2281 cgacctatcg cgttaaatat tttgtatact cttctgtatg aagcaatcgt ttagcatttt
     2341 cgacacgttc ttttgtaggt ggttcgatcc cttctaatgg atatttcaaa ccaagtgttt
     2401 cccacttgta ttttcccatc gtatgataag gaagaatctc tactctgtct acatttgaca
     2461 aggtttgaat aaagttgtct agtcggatca aatattcatc ataatcactt cgctcaggaa
     2521 ctagtacatg gcgaatccaa accggtttgt tgatttttga caaatattga gccatttcta
     2581 aaatattttc attgtcccaa tgcgttagtt ttttatgctc ttcattatcg atgtgtttga
     2641 tgtcaaataa cagtaaatct gtatacttca tcaattcttg aaaacgtgaa aagaaaggtt
     2701 cttcatacgt aaaaggtttt ccgcatgtat ccaatgtagt atgcatcttc cgctcttttg
     2761 cttttttaaa tagttcgatc aaaaaatcga tctgcaatag aggttctcca ccactaacag
     2821 taataccacc tttatctccc cagtaaggtc tgaagcgttc tgctttatct aaaagttcat
     2881 ctgctgtgta ttctttccct ccgccaatat tccaagtatc tggattgtga caaaattggc
     2941 aacgcatgcg gcatccttgc ataaacacga caaagcgcag tccaggtccg tctactgaac
     3001 cgaaagtttc gatagaatga acatagccga ttgttttttc ttccatttaa gttcacatct
     3061 tttctttgat ttcattttaa gtacaagtta gctgtcgaag cgaagctttc tgaacaaaat
     3121 ttttcgcaat tgccgcttcg ttatgtgtaa aatttacagg gacggaacat acaagtcctg
     3181 cccctgtaaa tcgtttcttt caaataagca tactgattat tcgattttga tgcgatggct
     3241 attcaacgtc ctttcgacgc taagtcgaaa aacttttttc acgaactttt tacattctgt
     3301 cgtgtgaagt tctcatcaag acatctaatt gttgttctct tgtcaagtca cggaatttaa
     3361 ctgcataacc agatacccgg atcgtcaagt tcggatattt cattggattt tccattgcat
     3421 cacgcaataa atcattactg aatacattga cattcaaatg ataagctcct tttgagaagt
     3481 atccatctaa gacattacgc aagttttcga ttcttgtatc ttgttctttt ccaagacctg
     3541 aaggattgat tgtttgggta tttgaaattc catccaatgc acagccgtaa ttcattttag
     3601 ctgttgagtt caggctagct aataacccac tttgttccgc attgtaactt ggatttgctc
     3661 ctggcgcaag tggttttcca gcttcacggc catcaggagt atttcctgta tttttaccgt
     3721 aaacaacatt tgaagtaatc gtcaataatg aagttgttgg ttttgagttg cggtaagttt
     3781 tatgacgttt gatttgtccc atgaagtatt ccaagatcca tgaaactaat tcatctgcac
     3841 ggtcatcatc atttccgtat ttagggaagt cgccatccac ttcaaaatca actgctattc
     3901 ctttttcatt acggattgct tttacttttg catatttgat cgctgacaag ctgtctgccg
     3961 catgagagat cccagcaata cctgtagcaa atgtgcgttg caagtcagaa tccatcaaag
     4021 ccagctgagc tgcttcataa gaatatttat catgcatata atgaatgatg ttcaacgtat
     4081 tgacatatag ttctgctaac caatccatca tattcatgaa gttatgtttc acttcttcaa
     4141 agtctagtat ttctgatgtg attggacgat attctggtcc aacttgggcg ccagtcactt
     4201 catcgactcc gccattgatg gcatataata gtgttttagc caagttagca cgagcgccaa
     4261 agaattgcat gtcttttccg acaactgttc ctgatacaca gcaagcaatc gcacaatcat
     4321 cagaacccca ttgttcacga agctgattat cattttcgaa ttgaatcgaa gaactttcga
     4381 tagcgattct gctagcatat gttctgaatc cttctggaag gtgttcagaa tacaaaacag
     4441 tcaagtttgg ttctggtgaa ggtcccatat ttgttaatgt atgaagcaga cggaaatcat
     4501 tctttgtaac taatgaacga ccatctaatc ccatacctgc aatggataaa gtagcccaga
     4561 tagggaatcc tgagaataat tggttatatt caggtgtacg cgcgaatttg accatacgta
     4621 atttcattac taagtggtcg atcaattctt gtgcttcttt ttctgtgatt actccattat
     4681 ccaaatcacg ttggatatag atatcaagga atgctgagat acgtccgatt gacatcgcag
     4741 caccattttg tgatttgatt gctgctaaat atccgaagta tagccattgg acagcttctt
     4801 ttgcatttgc agctggttta gaaatatcat agccatattt cgcagccatt tctttcattt
     4861 gcgctaatgc acgatattgt tcagtgattt cttcacgtaa acgaatcact tcttcactca
     4921 ttgtatgata accagtcata tcatgatctt ttttcttttg ttccatcaaa tagtcgattc
     4981 catacaatgc cacacgacga tagtcaccga tgatacgtcc gcgaccgtaa gcgtctggaa
     5041 gccctgtaat gattttgttt ttacgagcag cacgcatttc agcagtataa gcgtcaaata
     5101 caccttgatt atgtgttttg cgccagtcag tgaaaatatg tgtcaattca tcgtttggtg
     5161 tatagccatt gctttttagc gcattgttag ccatattgat tccgccgaac ggcatgaatg
     5221 cttgtttcaa aggtttgtct gtctgtagac cgacgatttt ttccaaatct ttatccaaat
     5281 agcctgcatc gtgtgaagta atagttgata cgatttctgt gtccatatca tagacaccgt
     5341 tacgctcgaa ttggatatca tttaactttt gcaattcatc ccataatttt tctgttgctt
     5401 ctgttgggcc ttccaaaaag ctgtcatcac cgcgatattc agtgtagtta ttttgaataa
     5461 agtctctagt gtctacgcct tctttccatt tggtaccttt aaatccattc cattgctcca
     5521 ttgtatggac ctcctaaatt tctgttttat gtaacagttt ttacgtgatt agtataacac
     5581 agcaccctat ctttgcaagg gttttcttgt tttttattta acaattttta tgttttattt
     5641 acatatattt aaatcaacgt tgacatacca acatttaagc attgtgaaca ctttcatctt
     5701 tttttgcaaa cgtataagga tttattaaaa atgaatgaaa aagagacgcc acctgtatta
     5761 atacagatgg cgtctcttca ttaaaatact acaacagtta cgactcgatg aaaatctcag
     5821 gagcatcgat aacttcgatg acttctccac cttttttctc atccatcgca aatgatccat
     5881 tcgatgttcg atcacctata gggaaatcag ttgggtcaat cgttaactgc tgcccttttt
     5941 gattcatcaa ggttatttct tttgatcttc cttccgacat atagataata cggtgcggat
     6001 tttttttcag ctcacgcatg accgtcactc cccgcttcgc acgtgaagtt tggctgattt
     6061 cttgagcaag catgcgttta acagctcctc gctgagtaac gattacgatc ggcgtatcac
     6121 cttctgctaa cacaagcagc ccgttgataa tcctatctcc atctttcaaa ttcatagact
     6181 tcactccagc tgcctttgct ccaacaacgg gaacatctgt taaaggatag cgcaaaccca
     6241 ttccttgatg cgttactaag aaaacatcta agcctggctg atcttctttc agatacacag
     6301 caatcagttc atcttcttcc gcctttagct tcatacagct gagcggacgg ctcttatagg
     6361 tgcgccatgg ttcgaactct gtcattcgtg tttgcttgat caatccattc ttgctgatga
     6421 atacaaatgt tttctctgct tgaatttttt gatatgggaa aacagctaaa atcgattcgt
     6481 ctacagacag attggagatt gtttgagaaa tgtgctcacc agcatctttc catttcaaat
     6541 cgggtagttc atggacaggg cggtagatca catttgcttt atttgtgatc agaagaacat
     6601 ggtccaatgt actcaattca cctgagtata gcaagtagtc gccttctcgc atcccgattt
     6661 cttctggttt ggaagcacta taagaacgaa ggcttgttcg cttcacatag ccttctcgcg
     6721 tcacactgac aattacgtct tcttgtgcca ctaagacttg cgtatcaatt ttgatttctt
     6781 cgatttcgtc ttcgattgtc gttagacgag cggaagaata attttttttc acttctcgca
     6841 attctttttt cataacagag aaaagctctt tgtcatttga aaggatttta ttcaactctg
     6901 tgatttgcgc aatcaagctt tcggattcct tttgaagctc tgtgatatcc gtgttcgtca
     6961 aacggtataa ttgcaatgtg acaattgctt ctgcttgttc ttctgtaaat tgaaagacgt
     7021 cgactaaatt ctttttggcg tcttttttgt ctttactttc acgaatcgtt gcgattactt
     7081 catccagaat cgataaagct ttcatcaatc cttctacgat atgctgtctt ttttgagctt
     7141 tggctaaatc gaattgacta cgttttgtta tgacttgctt tcgatgctcg atataacttc
     7201 ttaaaatgtc tttcagtcca acttgatgcg gagtcatatg gtcgatagcg accatattga
     7261 aattgtaatt gatctgtagc tcagtgtttt tgaacaaata attcaaaatt ccttgagcat
     7321 tggcgtcttt tttcaattcg acgacaattt gcagacctgt tctatcactt tcatctcgta
     7381 cttcagctat tccatcgatt ttcttattca aacggatctc atccattttt ttcaccaagg
     7441 tcgctttatt tacttcatat gggatttctg tgatcacgat ttgctgttta ttacccttca
     7501 ttgcctctat ctttgttttc gaacgaagaa taacttttcc tcgtcctgtt tcataagctt
     7561 ttttgatttc atcttttcct tgcaagatcc ctcctgttgg aaagtcaggt ccaggaatat
     7621 attccatcaa tttttcaagg ctcgcttgtg gatgatcgat catatagatt gtgccatcga
     7681 tcacttcagc taagttatga gtcgggattt ctgttgcata tccagcagat atacctgtcg
     7741 atccattgac gagaaggttt gggtatcgag ctggaaggac tgttggttct ttttctgtat
     7801 catcaaaatt ccacacgaga tcgactgttt ctttctcgat atctgctagc atctctccac
     7861 ttaacttgga taatctggct tcagtataac gcatcgcagc aggtggatca ccgtccatac
     7921 tcccgttatt cccgtgcatt tcgatcagta cttcccgcaa cttccagtct tggctcatgc
     7981 ggaccatcgc ttcataaata ctgatgtcac catgaggatg ataattcccc atgatatttc
     8041 cgacagattt tgctgattta cggaatcctt tgtcatacgt attgccatct ttgttcatgg
     8101 caaataatat ccggcgttgc actggcttca atccatcacg gatatcaggc agtgcacgtt
     8161 cttggataat atatttggaa taacgtccga agcggtcacc catcacttct tctaaagtta
     8221 attcttgtac ttcttgtcga ttttccatca ggtgacctcc tattctactt caaataactg
     8281 attgttttca tttttatctg ctcgttcttc atctaataaa tcattagaaa ctgaaggaga
     8341 gatttcagta tcctcttttt tatctaagat actgccatct tcttcgagcg taaattgcac
     8401 gtgattttcg atccattttc ttcgaggttc tactttgtca cccatcagtg tggttactcg
     8461 acgttcagct tgcgcagcat catcgatacg gacgcggatc aacgtacggg aagtcggatc
     8521 catcgttgtt tcccacaatt gttccgcatt catctctcca agtcctttat atcgctgcag
     8581 catatatcct ttgccgactt ttttaatcat tgctgccaat tcgtcatctg tccacgcata
     8641 ttcaatgact tgcttctttc cttgtccttt ggatacttta tataaaggcg gtaacgcgat
     8701 atatactttc ccagcttcaa tcagcggttt catataacga taaaagaaag taagcagtaa
     8761 tacttgaata tgggcgccgt ctgtatccgc atcggtcatg atgattactt tgtcgtaatt
     8821 acagtcttcg atagaaaact caggaccgac tcctgcacca atcgtataga tcatcgtatt
     8881 gatttcttca tttttcaaga tatcttgcat tttagctttt tcggtattga tgacttttcc
     8941 tcgtaaaggt agaattgctt ggaactttct gtctcttcct tgtttagcag atccgccagc
     9001 agaatcccct tcgactaaat acaattcatt tttcttaggg tttcgggatt gtgcgggtgt
     9061 caattttcct gaaagaagag attcgccttt tttacgcttc ttcccatttc ggctttcttc
     9121 tctggcttta cgtgctgctt ctctcgcttc tctggctttg attgctttac ggaccaacat
     9181 ctggctcatt tcgctatttt cctgcaaata aaagcccatt tgttcactga tgacgttatc
     9241 tactacggta cgagcaactg gcgtaccaag tttttctttt gtttgccctt caaattgcag
     9301 aagattttca ggtacacgga tagaaagtac agcagctaac ccttctctga aatcactacc
     9361 ttccagattc ttgtcccgtt ctttcagcaa accaactttt ctcgcatatt cattataaga
     9421 tttcgtcatc gccgctttca taccagcttc atgggttccc ccatcttttg tccgaacgtt
     9481 attgacaaaa gataaaacat tttctgaata accgtcattg tattgataag cgacctctac
     9541 ttcgatacct tctttttctc ctgaaaaata aacaactggt gtcaatgtat ctttttcttc
     9601 attcagataa tcaacaaatt ctttgattcc ttcttcgtaa tgaaaaattt ccttgactgg
     9661 ttcttctcca cgaagatcag aaagagagat tttaacgcct tttagaagaa aggcagattc
     9721 tcttaaccgt tcggctaaaa tttcatatga aaatttcgtt gtactgaata ttgtatcatc
     9781 tggtaaaaaa tggacagtcg tcccgtttgc tttttttgtt tttcctatct ttttcaaagt
     9841 accgtcaggt ttaccgccgt ttttaaattt ctgctgatac tctacgccat ctctaacgat
     9901 cgttacaatt agccatttcg ataaggcatt taccacactg gctcctacac cgtgcagtcc
     9961 accagaagtt ttgtaccctc cttgaccaaa ctttccgcca gcatgcagca cagtaaaaat
    10021 cacttcaact gttggtattc cggaagcatg cattcctaca ggcatcccac gtccgctatc
    10081 tgcaacagtt atactattgt cttcatggat ggtcacatca atctcactgc cataacctga
    10141 taatgcttcg tcaactgcat tatcgacgat ttcatagact aaatgatgca agcctcggct
    10201 gtcagtggat ccaatgtaca tccctggtct ttttctaact gcctctaatc cttccaatac
    10261 ttggatggac gagtcattgt attcgttgtt tacttttttt gccaaggcaa acgctccttc
    10321 aataatactt tgcggcaaaa ccgcaccacg attatcatac tagaaaaggt cgtgaaaaaa
    10381 aagagggtag acgagaaatc aattttaatt atctcctaac cgctttttcg acattcccct
    10441 ctatcgatca tctcttcttt gtggtcattt ttatttttgg ttttctttca acttaccaag
    10501 ttcaatctta atacaacgat ccattacgat atctgtacgt cctgcttttc ttagcatctc
    10561 agctgcttct ttgttttcaa tccctaattg tgcccagaac actttagcat cagttttcaa
    10621 aaaatcacct gctatttcag gtaaaaattc actccgtcta aatacatcta cgatatcgat
    10681 tggatcatcg atttctgtca aattcgcata gactttctcg cctagaattt cttcaccagc
    10741 caataatgga tttactggta tgattttata tccattgtct tgcaatacct gtgcaaccat
    10801 gtaacttgtt cgttctggtt tattgctcaa gcctacgacg gcaatatttt ttgcttcttc
    10861 caaatagttg aaaatcgttt cttgtgttgg attttttaca gacatttctg ccaccccttg
    10921 cttttctttt tattccatct attatacttg tatctttcat tttttcaatt gttcctgtta
    10981 cctattcatg ctaaaataaa ggatagtttt caacttgaaa gagggtaaaa aatgaaattg
    11041 atcttattac ttattttagc atatctttta ggttctattc cttccggtgt ctggattggc
    11101 aaattgtttt tcaaaaaaga tatccgacaa cacgggagcg gtaatactgg aacgaccaat
    11161 acatttcgtg tattaggcaa aacagccggg attgttgtcc tgcttatgga tattttaaaa
    11221 ggtacattgg ctacttgttt gccgatgatc ttccaccttt ctgggattaa ccctctttgg
    11281 tttggggtat gcgccattct tggccataca tttccgattt ttgctgggtt caaaggagga
    11341 aaagctgttg cgacaagtgc aggaatgctt ttaggattta atcctgtatt ttttgtttat
    11401 tcttgtatca tctttatcgt tagtttattt tgtacaagta tggtgagttt gagcagtatg
    11461 atcagtgcag tattgatcac tttatctacg atcgtccttc cttatgtagc tcctgtgatt
    11521 ttggctaagc ccaattggtt actgacgatc attgcctttt tagtttcttc ctttatcttt
    11581 tatcggcata aagacaatat caagcgtatc agaaaccata ctgaaagtcg aatcccgttt
    11641 ggtttgaata gcagcaagaa aaaatcctga tactttttca ttccaaagag ataaaacggg
    11701 agtgtgacaa atcgatgtca cactccctta ttacttacag gatagctttt ttacacacct
    11761 tggtaaacgg tgttccctgc taacttctcg gtcacaacct cttacttcac tgtgatggaa
    11821 tattttgtct tgaagatttc atgggcacct agttgattga tccctacttt ttccatcaag
    11881 tttccactac tgttcaccgt gtcagcgatt ccgcaccacg gttcgataca aacgaaaggc
    11941 gcttcttttg gataaggaga ccagatccct acataaggca tatctttata gcttaaagtt
    12001 acgctatggg aagatttttc acttcgaatg gcgtacgcat ttagacccct tgtttcaaaa
    12061 accaaggcat cttgctcaaa aagttcacga ttcaaagcca gattcgtatt tgtctgacct
    12121 aacgtctttt gatccatatc gatgaatggg cctttcaatg gtaggcgtaa tcttgatttc
    12181 aatggagaaa atgccagata gaaatcttca aatgacaatc cttcttcaag aggtacattg
    12241 aaagcaggat gtccaccgat agaaaagaac atttcttttt caccggtatt tgccacttga
    12301 taatgaaccg tgatgccgtc tcctcctaat tcatagctga tcactaattc aaaatcaaat
    12361 ggatactttt ctaatgtctc cggcgtactc cttaagatga aactagccat ttcttgtcca
    12421 tgttcaatga cttcaaaatc catatcccga gcaaatccgt gttgccccat ttgatatgtt
    12481 ttcccttgat aaacatactg atcatctttt aaacgtccaa caaaagggaa taaaaccggt
    12541 gcatgtctgc cccaatacgc tggatcacct tgccagatat actctaaacc atgttctttt
    12601 gatttcagac tgatcaattc cgcaccgtgt tcagcgattt cggcgatcag ctggtcattt
    12661 tgaataataa cagtcattgc cattcctcca acttcgactt actttttaaa atgggaaggc
    12721 tggaacaaac atttctatct gccccagtcc ccttacttct aataactgtg ttttgctaac
    12781 tcgtctttaa aacgggaatt caatactttt aaataagttc ccttcatgcc taacgaacga
    12841 gattcaatga ttccagctga ttccaatttt cgtaaagcat tgacgatcac agaacgagtg
    12901 atgccgatac tatcggcaat attagaagca gtcagacgac cttcatctcc atccaatgct
    12961 tcaaagatag cttgtacagc cttcaattcg ctataagata atgtgttgat tgccatttgg
    13021 acagccgttg cactccgtac atctgcttcg atgcttcgag acttctgata aaggatctgc
    13081 ataccgacaa ccgtagctcc atattctgct aagactagat cgtcatctgc aaacgattgt
    13141 tccatacgag ataagataat cgtacccaaa cgttcgccag ctccaaagat cggaacgaca
    13201 gtcgtcagac caaatggata tttctctctt gactctaccg gaaatgcagt aatatcacta
    13261 gtaattggga tattagcttc agttttcatc aactgtgcca ctgtatcagt ataagattca
    13321 ggaaattgtt tctcaacaaa cattgttttg attcgttcat tgttcacatc gtgacgttca
    13381 ttataaccta gtaatactcc gtcacttcct atgatatagg tgttgctgtc aagaatacca
    13441 cctagtgtga cagccatttt atcgtaaggc aaatctgctt gcatgtcaaa agtattcttt
    13501 tgctgcaaaa gtgtgtttat cttgcgggtt ttcgttagca agtctgtcat tgtcgtcctc
    13561 cgatttttta taaaatatag cgacttaaat cttcatttgc cgcaattgag ccaagtttgt
    13621 catccacata tgcttctgtg atcgttattt ctcccatttg catatctggt gcttcaaata
    13681 atagatcttc tagtagtttt tccaaaatgg tatgcagtct gcgtgcgccg atattatctg
    13741 tgtcacggtt gacatcgaaa gcgatttgag cgatccgttc aattgcttct tttgtaaacg
    13801 taactgacac attttctgta ccgatcaacg cgacgtattg ttttactaaa gcattatttg
    13861 gttcggtcag aatcttcaca aaatcttcag ctgtcaaatc atctaattct acccgtatcg
    13921 ggaagcgtcc ttgaagttct ggaattagat cacttggttt agacaaatgg aaagctcctg
    13981 aagcaatgaa taggacgtga tctgtttgga tgatcccgta ttttgtgttt acttgtgaac
    14041 cttcgacaat tggcaatatg tctctttgga cgccttctct ggatacttct ccagattgct
    14101 gtgatttaga agtgatctta tcgatttcat cgataaaaat aattccactt tcttccgcta
    14161 atttgattgc ctcactatgg atatcagcat ccttgactaa ttttgctgat tcttccttga
    14221 tcaataactc taaagcttct ttgactgtta ccgtccgttc cacttttttc ttcggtgtca
    14281 aagcacctag agtttcattt aggtcgatcc ccatttgctc caatccattg ttcatcattg
    14341 gtgcaggttt cttttggtct tcaacttcaa tggttacttc acgactatcc agtaaaccct
    14401 tttgtagttg ttccaagact gtacggcgat tgattcgaat atcttctgtc acttcttctt
    14461 gcggttcagc ggtattttgc tgactagcat taaaaagatt catcatttgt tcaaattgat
    14521 tagtggattg tttctgttct tttttgatac caggtaccaa gatttttacc aaacgttttt
    14581 ctgcattttt tttagcactt gtgtatacac ggctgtactg ctctttttct acgatttgga
    14641 tcgcattttc tactaaatcc cgaaccattg attctacatc tcttccgaca taaccgactt
    14701 cagtaaattt tgttgcttcg actttgacaa atggtgcatt gacgattttt gccaaacgtc
    14761 gtgcgatttc tgttttaccg acccctgtag gtccgatcat cagcatattt tttggtgtaa
    14821 tatcttgttg cattttttca tctaactgca aacggcgata gcggttacgc aaagcaacgg
    14881 caacagattt ttttgctgcg tgctgtccta cgatatattc atctaattca cttacgattt
    14941 gtcttggtgt ttgttttaat tcatacatat ctactcacct cttacagttc ttctacgata
    15001 atattatgat tagtaaagat acagatatct gcggcaatat ttaacgcatt ttctgcaatt
    15061 tctttcgcac tcatgtcgtt ttgagcatag tttttcattg ctcgggctgc agccaacgca
    15121 aaattccctc ctgatccgat agctaagata ccatcatctg gtgtgataac ttctcctgta
    15181 cctgaaacaa gaagcatttc tttatcattc attacgatca gcatcgcttc aagtttttgc
    15241 atagattgct gtgtccgcca ttcttgagct aattcgacag ctgcgcgttg tagattcccg
    15301 ttatattcgt ttagtttgcc ttcaaatttt tcttctaaag taaacgcatc tgcaacactt
    15361 ccggcgaaac ctacaacaac ttctccattg tagatacgtc gtactttctt tgcagtgcct
    15421 ttcatgacaa cagattcacc cattgttact tggccatctc cagccatagc aaatttcccg
    15481 tctttttcga cggcacagat agtggttgaa tgaaatggtg attcgaccat gtttgtttcc
    15541 tcctaaattt gttcttttcc ttatttttac gctcttggat ggaaagaacg atagtttttt
    15601 tgtaaacttt cttttgttac atgggcatag atctgggtag tcgataaatt ggcatgtcct
    15661 agcaattctt ggactgtccg caaatctgcc ccattattta ataaatgagt agcaaatgta
    15721 tggcgcagca tgtgaggatg gatatcacta tccaaactgc tttttttgat catttgattc
    15781 aagacatatt caattccagt aggcgtgatt ggttttccat gatgattcac aaaaacgata
    15841 tcatgctctt cgtgatattt tgtcatcaat acctggcggc cttccctgaa atattcttgc
    15901 agagcatctt gagcaaaaga acctagagga gcatatctct ctttgttccc tttaccatgg
    15961 atcaacatga cctctgaatc aaaatcaatg tctgataccg acaaactgct acactcactt
    16021 aaacggatgc ctgacccgta aaggacttcc agtaaagcgc ggttcctcaa atccaaagga
    16081 ctatctcctg ctacgctgtc aaaaagtgct tgcatttcat tttcataaaa aaaacgtggc
    16141 agtctcaaat ttttcttttt gagatgaacg taagaaaatg gattttcttt tatcacttct
    16201 tctttgagaa gatactgata aaacgaacgt aggctggcta tcttgcgact gatagaattt
    16261 ctgctatagc taggacgatg atcagctaat gcactcaaat agacacgaac cgtaaaatat
    16321 tccgccttca gcagatcagc gtctccagat tcctccaaaa aatgtttgaa atggagaatg
    16381 tcttcttcat atgcttcttt cgttttttct gaatatcctc tttcgacaat caaataacgc
    16441 aaaaattttt gttcccacgt ggcttctgtc attttctcac cttccttttg tcccagagaa
    16501 taatttagca cagcgtttat aaaaagacaa ataaattgtc agaatttaac caatatttct
    16561 aagtttgttc acaattggcg caattcctca cattttgttt gagaaattct taagaaaaac
    16621 gcaagaaaaa gagcgagact atccgtctca ccctcttcat ggagtttcct tactcatttg
    16681 ggattgcagt tttttctaat tctgttttta ttgcctcatt agcttttaaa gctcgttgtg
    16741 caattgcttc atatcttgct tttttgtctc gaatgcgctc tggcagttca ggaaacagac
    16801 catagttggc attcatcggt tggaaatgtt ttccttccgc atgagtaatg tagtaagcca
    16861 tacttccaat agctgtttct tgtggaaact cgactggttc catttctttt gctaagcgtg
    16921 cggcgttgat tccagcaagt agtccgctcg ctgcactttc aacatagcct tctacaccag
    16981 tcatctgacc tgcaaagaac agatcttcgc gtttcgttga ttggtaagtc ggtttcagca
    17041 attctggcga attcataaag ctgtttcggt gcatcacacc atagcgaacg aattcagcat
    17101 tttctaaccc aggaatcatc cggaaaacac gtttttgctc gccccatttc aagtgagtct
    17161 gaaagcccac gatgttgtat aaagaagctg cagcattgtc ttgacgcagc tggataacag
    17221 cataaggacg tttgccagtt ttaggatctt ccaatccaac tggtttcata ggtccgaaaa
    17281 gcatcgtttt gaatcctcgc tgtgccatta cttcaattgg catacatcct tcaaaatatt
    17341 tttctttctc aaaagattta agttctgcca cttcggcatt gattagtgcc tcatggaaag
    17401 ctttgaattc ctcttctgtc atcggacagt tcaagtaagc tgcttctcct ttgtcatagc
    17461 gggacttcaa atatacttta tccatatcga tcgtagcttt gtctacgatt ggtgcagctg
    17521 catcatagaa ataaaaacca tctgaaccat tgaatgcttt gatgctttct gctaatggat
    17581 gagaagtcaa aggtcctgtt gctactacgg tgattccttc cggtatttca gtaatttctt
    17641 cgtttatcac tgttactaat ggatgctctt tgacttttcg ggtaattgtt tcagaaaaag
    17701 aatcgcggtc aacagctaag gcaccaccag ctggcactgc tgtttcatct gccgaagtga
    17761 ttactaccga attcaatcgg cgcatttctt ctttcaataa ccctactgca ttagccaaac
    17821 tgtttcctct taatgaattg gaacatacta gttcagcaaa attacttgtg tgatgcgcct
    17881 cagtcgattt gactggacgc atttcatata aacggacagg gactcctgct tgtgcgatct
    17941 gccaagcagc ttcacttccg gcaagccctg ctccaacaac atttaccatt ttcaatgaaa
    18001 gaacctcttt tctattattt ttggatgtct tcttcgtaat ctccatttac acagactact
    18061 tgtttaccgc ctttgacttt cttctcgacc agatatccgt cacattttgg acaatttctg
    18121 cccactggtt tatcccaaga agtgaattca cattctggga aacgggaaca accatagaat
    18181 agtctgtttt tcttagattt tcgttcaatg acttgtcctt catgacatac tgggcaagtc
    18241 acaccgattt cttttacgat tggtttcgta ttccgacaat caggaaaatt actgcaagca
    18301 taaaacttcc catatttccc taattttatc accatcggat gtccacagac atcacaatcg
    18361 aatcctgctg gttcatcttt gatttgaatt ttttcgattt tttcttccgc attcgtcagt
    18421 tctttttcaa acggtttata gaaacgatca accactttga cccacgcttc cgtgccttct
    18481 tcaattttat ccaaatctcc ttccatttct gctgtgaaat ggatatcgac gatttgaggg
    18541 aagaattcac agattaatga attgacaatt tctcctagtt cagtcggctc aaatcttttt
    18601 tgtgtcaatt ttacatagta gcgtctttgg atcgtatcaa gtgttggtgc ataagtggaa
    18661 ggtcgcccta ccccgttttc ttctagtgca cgaatcaacg tagcctcact aaaacgtgct
    18721 ggtggttgag taaaatgttg ttttggttca atatcaacag attggactac atcgccttct
    18781 tcaagttctg gaagaatatt ttctttctct tctttgccgt catctctacc ttccacatac
    18841 acttgcatga atcctttgaa tttgattttt gaaccgttcg caatgaagat gacaccattt
    18901 tgttctaatg tcactttcat tgtatctaaa atagctggtg tcatctgact agccacaaaa
    18961 cgtgaccaga tcaatgtata aagtttcaat tgatctttat ctaaatattg tttgagttca
    19021 tctggtgtac gaagcacact cgttggacgc acagcttcat gggcatcttg tgcgccttga
    19081 gcatttttga tctttcggtg accatgtcta gaaaattctt ttccatacgt atcttcaata
    19141 aatgctgcgg cctctgcttt tgctgtatcg gcaatccggg tagaatcagt acgcatgtat
    19201 gtaatcaagc cgactgttcc ttgtttaccc aaagtgattc cttcatataa ttgctgtgct
    19261 accatcatgg tttttcttgt tcgaaagttt aacttacggg cagcttcttg ttgcaggcta
    19321 cttgtagtga aaggattcgc agggtttctt ttgcgctctt tcttttctac ttttttaact
    19381 tcgaagtctg ttccatcaat tctagcagtg acttctttta cactttgtgc atctggtaat
    19441 ttctttttct taccgtcgat tccccagaaa tttgctttga atttcttgcg agctttttta
    19501 aaattaccgt cgatgctcca atattcttct ggaacaaatt ctcggatctc tttttcacgg
    19561 tcaataataa tttttaaagc gacagattgg acacgtcctg cacttaaccc tttcttgacc
    19621 ttacgccaga gaataggact gatcgaataa ccaacgattc tgtctaaggt acgacgagct
    19681 tgctgtgcat ctactaaatc tacatcgatc gttcttggtt ccttaaaagc tgctttgact
    19741 gcgtcttttg tgatttcatt gaagaccacc cgattttttt ctttcagatc caacccaagt
    19801 aggtacgcta aatgccaagc aatcgcttct ccttctctat ccgggtcact tgccaagtaa
    19861 actttttgag ctttttttgc tgcggctttc aggcttttga tgacatcgcc ttttccgcgt
    19921 atagaaatat aatggggttc ataattgttt tcaaaatcga tacccatttt acttttcggt
    19981 aaatctctta tatggccaac actggccatt actttatagt ttcgtccaag atatttctca
    20041 attgtcttgg cttttgcagg tgattctaca atcactaaat atttatacgc cattactgaa
    20101 tggactcctt tccattgtcg tgttcctact attaaaagag gattcataca aatcgtagct
    20161 aatcatatcc attcagaaaa cgtttgtcaa gaaaagcaaa tgaaaagtat ggtgttattc
    20221 tttaaattct gccaagatat cttcacccga aaccgtgcaa actgcaccgt cttggatcaa
    20281 atgatgacat ccggtagaat gttctgttaa aatatttcct ggaacagaaa aaactgttct
    20341 tccaagatcc aatgcttgct gtgctgttat cagagagccg ctttttttct tggcttcaaa
    20401 gacacatgtt cctttagaca aagctgcgat aatccgattt ctcattggaa aacaatgctt
    20461 tttcgggccg gattgaggag gatattcact caaaagaaga tgatcatttg caatcacctc
    20521 ttgcaagtca cgattttcag caggatagac ttgctctatc cctgttccaa tgactgcgat
    20581 tgtccgtcca ccatgagaga ttgctgcttg gtgaccgtat gtatctatcc ctttcgcgca
    20641 gccactgacg atcacatatc cagcttctat cactggtttt agtaaataat aaactgtttt
    20701 tttggctaaa gcagaagtgt gtcttccacc aacaacgctt aacatgttac aagaaagtaa
    20761 agatagatca ccattataaa acagtacaat tggcggatag gtcaagtgaa gcagctgagg
    20821 gggatatttg ggatcaaagc aagtgataaa cgactgtttc gattggattt tgccccagtt
    20881 agcgtgaatt tcctgccaac ttttgcgaaa cagctcccgg tgtttggtta ttttacctat
    20941 cttaatgatg tcttcaaatc cgatttcttt ataaggatac tgcatcatcc agttagcaag
    21001 tgcccatttt ccttgactac tgattccttt tgtataagca agtttcaata aattttcttc
    21061 tatttgatac atcataaaac cgcctttcgt ctctctttct aataagatac gtaaaagcgg
    21121 ctgtttttta ttgtaaatat ttttttacag gtgcaaaact tttacgatgg attggcgtaa
    21181 ttccttgtcg ttcaagtcct tctaagtgtt cctttgttcc ataacctgca ttatgcgaga
    21241 aaccatatcc aggataaagc acgtcatatt ctttcatcaa ttggtctcga tatactttag
    21301 caatgatact tgcagcagca atcgacacag atcgtgcatc ccctttgatt attttttcct
    21361 gctgaatagg caaatccaag gtcatcgcat cgatcaacaa atagcttggt acaatggaca
    21421 atttttctac tgcttggatc atcgctaatt tacttgcttc ataaatattc acatcatcga
    21481 ttgtctgctg atcaacgatc ccgatcccga ttgccagggc tttttcttga acttgttgat
    21541 aaagaagttc tcgctttttc tctgataatt gtttggagtc attcagccct aatatgatct
    21601 catttttcgg caagataact gcagctgcca cgacaggtcc agctaatggt cctcttccta
    21661 cttcatcaat gccggcaatc cattggtgac cttgtgcgta cccttgcttt tcaaagcaaa
    21721 acatttcttc atatttttcc ttaattaatg cttccttttt caatcgtcgt tcaaaagaag
    21781 ccagtgcttg ctgcacgcct ttgcgtggat catttcttaa tgattctagt gtaggatcat
    21841 ctattctctg tatgcttttc aatcgttctt tgatactttg gatggattca gtcatcactt
    21901 ttttgctcca tttcttcgca acgatccagt gtgtatcgtc caagttttcc ttgacgaata
    21961 tcatgcacga ctaattcgct gccacgatca tagtcttctt tatacccacg ttttgctgta
    22021 atatccatca gtaattccgg tgccaatttt tcttcttgct cgattgataa accataacga
    22081 tcgctgattc ttcctgaata atatcgggca aaaaagtcta atccgtaaat cgctaaatca
    22141 tctaaatgga caagctggtc tttgatcgcg cctgtcagcg ctagtttttt gccgatttct
    22201 tcatcttcaa atttgggcca taaaatccct ggtgtgtcta acaattccaa ctcattacct
    22261 aaacgaagcc actgctgacc ttttgttacg ccaggcttat ttcctgtctg tgcaattttc
    22321 ttgcctacta gtcgattcat caacgtagat ttcccaacat ttggaatacc taagaccatt
    22381 gcccgaatag cgcgaggctt gatcccccga gcagcatctc ttgcccgttt ttctttcaat
    22441 gcttctttag ccgcaggtac tagaccttta attcctttac tttcttgtgc cgtgatagct
    22501 aatgcaagat ggccttgttt acgaaaatat tcttgccaca gctttgtctg ttgtggatct
    22561 gccagatccg ctttattcaa taaaacaagg cgaggttttt gctgaatgat ctgatctagc
    22621 attggattac gagaagataa aggtaatcgt gcgtctacca attcaaatac gatgtccaca
    22681 tatttcattt tttcagaaac ttctcgtcgg gcttttgcca tatgtcccgg aaaccattgg
    22741 atggtcattg ttctctccat tcctttcata ccggaatatt ccccggcttt acttgttttt
    22801 ttctttttca atataaattc acttttttat tatcttaatt gtcaaattaa gttagacaaa
    22861 tcatcaatac ttacgtaact caaaaatact tccaaaggtg ttcgataatt cagtgatttt
    22921 ctaggaatat tatttcgttt cgatgcgaca gattgaataa aagattcatc tactgaattg
    22981 aaatccatag atttcaataa accatctcta cgtaacaatc cgttagaatt ctcgtttagg
    23041 cctctttgag acggcgttcc tggatcagcg aaataaatgg caatatcatt ggcattactg
    23101 atctgtttcc aatttgaaaa ttcctttcca caatcaaaag tgatggattt gaatagattt
    23161 ttcggtacag attcaaacca atgatttaat ttgttttcaa tatcaatcgc ttgtctacca
    23221 cacggtttca atgtgatgat aacttttgat aatcgctcaa ctaaggtaat tacagcactt
    23281 ttatgtttca aacctacgat agtgtcacct tcaaggtgac caaactcatt tgagaattgg
    23341 ctataatctt tttcacgttc atgaatagag cgacggaaag cttgttttcc acgtctttct
    23401 tgatgtccat ttggtttacg cttgcctttc atcggtaagt cagaaggatt aaatagcccc
    23461 tttttgaaca tacgataaat ggtacgagca gaacaacgaa taggaaacgc tgcacgacca
    23521 acaatcacat cgggtgtcca tccttgaaca acctttcttt gaatatattc tgattgttcc
    23581 tcaggtaaaa caagcgggcg tctaccacag tttgatttgt ttttcttata ctgttgatag
    23641 tactctaagg ctgattttcc ttgcttgaag aacaggtata ctttatggat cgtttgtctt
    23701 gaacgattca agaaatgcgc agttttcgca acagattgat ttattttgaa ataagactct
    23761 atcattacaa gttcatccgt tgtaagatgg gtataggtca tttgtactca ctccttataa
    23821 ttctttggac ggaactattt tgagtgtagc acaaatgact tttttagttg tctagcttaa
    23881 ttttacaatc ggcgttataa agaaattcca tctattagtt aagtgaaagt gaaccagtcc
    23941 tcattctatc acatcttcac ttttttgatg atgcaaattt ttattttggt ttctatatat
    24001 gaatctaaat ttttgacagc gtttcctaaa taagagatga tagacgaaca aaacagaaag
    24061 gagacgaaac aaatgaatag tatggttttt gttaattttc ctgttgctga tgtcaatcgt
    24121 tccgttgctt tttatgaaaa gctgggtttt aaaaaaaatg aagaattttc tactgaagaa
    24181 gctggttcaa tggtatggga tgacactttt tggataatgc ttctctctca tgatttttat
    24241 aaaaaattta taaaagataa agaaatagct gataataaaa aaacaagtgg tgctttgatt
    24301 tcgtttagtg ttgaaaatgc ggatgaagta aaaaaatttg ctcaaattgc aaaagaaaat
    24361 ggtggtactt tccacgaagt agatatggga atgcctgaag aagaaatgtt ttcattagaa
    24421 gtccaagatc tagacggaaa tacattagaa cccgtatgga tgaagatgta agcagataac
    24481 taatctgacg ttttttataa caaaaaaaca aaggcggatg atacgataag tattctccgc
    24541 ctctattttt tttattcgta caatctcttt tttacagttt ataaaagcag tcttctcacc
    24601 ctttttttaa ttgttagatg ccagtgtatg taaatcacca atgtgtcatg acacattggt
    24661 gattttatgg tatcctttta ttattaactg acaaaggaga gaatattttg aacactttga
    24721 ttgcaaaaga cgtttttaga ttgcccgaaa tgaatgcaag attgttgtcg acctctgctc
    24781 ttttattagc ccttgatgtc atcttataca agatagcgat tggtcctgct tattttcaaa
    24841 tcacattcgg atttttaagt atggcgctta tgggttatct atatggaccg atctgggctg
    24901 gtttgcttga aatgttgtcg aacactttaa atttcacgat atttggttca ggaacgtttc
    24961 aagctgcttt tcttatcacg gctttccttg gtggatttat taatggctta tttttatatc
    25021 ataaaaagat aaacctgcct tccgtcatcg ctgcccaatt attgattatg atcccagtca
    25081 gcctttttat caattcttgg ctgatgtctg ttttgtttgg tgcagatttc aaagtaattt
    25141 ttctaggtag attcatcagg aatatgatcc aaatccctat tcaaataatg gttgtttatt
    25201 catttatcaa aactttagag gctagaaaag tgtttaaacg agctgtttga ttttacgtac
    25261 taaggtagta aactgatttt ggattaaaca ataaagcgtg cagtctcaac agactgcacg
    25321 ctttattgtt tctcactgga tccattttat ttcatcaagt ggataataga caaatcttgc
    25381 tttacctaga atatcagttt tattgatcgt gccaaacgaa cgactatctt ttgaaactct
    25441 tcgattatct ccaagtacaa aataattatc ttccggaagc ttggattgtc ccgttagctc
    25501 ttgtagtgta aagtccgtcg tataggatgc atttgcatga tctgatttca ggtttttagt
    25561 tagataaggt tccttaacta ctttcccatt gatcttcaat tgatcatttt ggtagctgac
    25621 attttctcct ggaagaccga ttactcgttt gatataaatc gtgccatctg ctaattgaaa
    25681 aacaacaata tcaaatcgtc tgatctccga aaatttttcc atcacaacca tatcaccttg
    25741 cttcaacacg ttttccattg aattgccttc cactggtacc gggatgaata aaaatccccg
    25801 caacatgaac gcaaggaata cagaaacaag taagtatttg aatacaagcc agaagcgatt
    25861 aatatagcgt tgtttttttg tcaatctcta tcaatccatc cctctttttt tcattctttc
    25921 tttattataa gaaaaatatc aaaaaaatgc gactagatag ctgaaataaa aataattctt
    25981 tcaattaggc tattggacaa aaaacttttt tgatcataaa gtacaaaact atctcttata
    26041 tgtcatgatt attttctaaa tatttttccc atgcttcgtc aaagatatcc atggacggca
    26101 aatagtcgac attcagttcc agataattag aaatttcttc gtagctagag ctgtgcttag
    26161 gaaattggat gtctttaccg gcttctgtag cgaaaacaga taaagcatca tgtgtgccac
    26221 cgcctcgcag tgtcattaaa taatgaaaaa agcttcgatt cacccttcgt tcctccaatt
    26281 ttcttcgata aaggcagccc ggcgtgcatg gtttctttta aaattttcag gatgcttttt
    26341 ataatactct tgatggtctt cttctgctag ataaaatggt tgggctggtt cgatttttgt
    26401 aacgattggc tgttcgaaac gtccactttc agctaactgt ttcttacttt tttcagcaat
    26461 gatccgctgt tcttctgtca aataaaagat caccgggcga taattgtcac cgcgatcctc
    26521 gaattgtccg aaagcgtcag ttggatcagt ttgctgccag taaagttcta ctaattgttc
    26581 atatgaaatc aatgttggat catagataat ctccactgct tctgtatgtc cagtcgtttg
    26641 gctttttact tgttcataag tcggatctgg tacatgccct ccagtatatc ctgacatcac
    26701 tgtatggata ccaggaagcg tgtcgaaagg ctggatcata caccagaagc agcctcctgc
    26761 aaatattgct cgttccatta tgattccccc tctaatacta ctcatccgat gattttttat
    26821 tttcttttgt cttgctttca gtactgctgg tgctagtttt gttggtattt gagttgtttg
    26881 aagcatcaga ggtatctttt ggcaaatata gactaaaccg aatgtcatca tttactaagt
    26941 cgattttatc tgctttaata tacatcccgt tttgcattcg gaattgatcc aacctaagca
    27001 tgatagtcag atcatctgta ttgacttcga tccaatctgg taatttatag tttctcttga
    27061 tcatattcat cacatctttg attggcaaac tcaatgttcc gatagaaaga cttctagctt
    27121 ttagttggac atttccgttt tccatcacat acggatcaaa atataaatag aaatcgatag
    27181 gaaaacctag tacttcgaaa gtaccattga gtagtgcttc attttccaaa taaaatttat
    27241 attcaatatc actatctttt tgatactcac taaggaaaaa gctgatgatt tgattgactt
    27301 tttctttatt tgaattgatc gttaagacag gatctccttg tctttccgta agagctggta
    27361 tttctgggag ttctggttcc cgattagcaa aaatacggct gcctaaaaat acggtcgaac
    27421 caagtactac cccaagaagg ataaggaagg cgaatttcca gtaatttgtt ttctttgttt
    27481 tgtttgaaga tacgtcagat cttgttttca tgtttattga tcatcctcct tctctaacca
    27541 ttggttttgt gtctcgatca gctgatctct cactgcattt gccatcaatt gataacctaa
    27601 attatttgga tggaaatgat cttcttcata gagcacatta tttttgatgc catttagatt
    27661 ttctgtcgtg ctagttgttt gatctgttcc ttcttctgtg acacctactt cctgattcaa
    27721 tccttgataa agcaaatcat tgattgggat aaagtagcag ttcggcattt tttttgtcac
    27781 cgtttctgtt cctttgttcc aattgttaac aattgtctgc atgtcagtga tctcaggaaa
    27841 gttcaaataa aatggattgt agatgcctaa aatgtaaata ggtgcggttt tattgaggga
    27901 tcgaaattct tccaatagtt ctgtgacatt ttcctgatag tttttcagtg gctttttaaa
    27961 ggaattgacc gttaacccga ataaattatt ttggataact ttcatcaaat cattaccgcc
    28021 aacagtcagt gtgataaaat cagctgtttt gatattgttt cgaatttctt cttttgtttt
    28081 tactcgcttt aagatctggt cacttcgttc accatttaca ccatagtttt ctgtttcaac
    28141 cgcggtcaaa ttataacggt cttttagatc atttgcgaca aggggaacga atcctccttg
    28201 cttggtttga tctcctactc cttcagtcag cgaatctccg atagctatgt agtgaagttt
    28261 ttctttttta tttggatctt ctctcacagt agttactggt ttcattagtg ggccagcttt
    28321 cggtatagaa gcagctaaac ctgcatagat gatgatcgca attaataatg ggaacaaaac
    28381 gaggatcaaa tgtcgtttcg ttaatttcat tttttcacct cttgtattcg attggttcta
    28441 atgttatcaa aaattttatc aaaaaggtag tctttatgaa aaaagaaaac aattattttc
    28501 ctaattttat aaagaaaaag agaataagct gcaagccttt caggctgagg gcttatcctc
    28561 ttttcgacta atctacataa tacatgattg caaacgcgtc tttaccagta tgcgtagcga
    28621 taaccggatt cgtatgcagt acagggatat gcatgtccgg gaataattct tgcaaaccct
    28681 ctttgaattc ttgcgccaat tcttttgcat ccgcatgtga aataccgatt tgtcgcacat
    28741 ttggcaattc tttcagttca tttttcaaat tattaaacca tttatcgaac gttttctttc
    28801 ctcgcccctt agttacaggg attaattcag aatgggcgaa attcatcacc acgcgtatgt
    28861 tcaaaacact agacagcaaa ccagttgttc ggctgattct tccacctttt accaagttat
    28921 ctagtgtgga tattccgatg tataattttg tattgtcttg tacttttttg acttcttcaa
    28981 ggattttctc tactgtttta ccttcttttg ctaattcagc tgctcgaatt acctgaaaag
    29041 ataatccttg gtcagtggta tcactgtcga taaccgttac tttcgttttt gttagattac
    29101 tggcttgtct tgctgcttct actgttccac tcaagccttt tgtcatatgg atcgaaatca
    29161 cttcactgcc gtcttctcct agctcatcat acatcgagat gaattctcca attggtggtt
    29221 ggcttgtttt gggcagattt tttgctttcg ccatcatttc cataaatctt tcgccgtcta
    29281 gttggtcatc atcgggatat actgttccgt cgatcatgat ggatagcggc atgacacgaa
    29341 tgcctaaagc atcgcgtaca tttttttcca tcgtacatga agaatctgtt acgattttta
    29401 catttgtcat tattttctct ctctctttct ttaacaagat gccttattca tgttaaacta
    29461 catgtaagac aatttctatt tttagtataa cagacaaata atgatttttc attaattaca
    29521 cctagtaaag gagccgtttt catggaaaaa acgaaattta atcgaacgta tgaaatatta
    29581 aatgaagtgt ttaatgcagt cacccatgga attgggactg gattgagtat cgcgggacta
    29641 gtggttcttt tagtaaaagg tgcccacttg cattcctccc tccatattgt ctcttatgct
    29701 atctatggat cgatgatgat tcttttattt ttaacgtcta ctttgtttca ttcattgatt
    29761 tttacaaaag caaaaaaggt gttccaagta ttcgatcata gttcgatttt tcttttgatt
    29821 gcaggaagtt atactccttt ttgtttgctg agtatccgag gatggctagg ttggacttta
    29881 tttgtattaa tctggttgtt tgcgattctt ggcattatat ataaagcgtt gacgttgcat
    29941 aaacgggaga gagtatcaaa agtatctacg atcatctata tttttatggg gtggctctgt
    30001 gtcgtggctg ctgttcctct ttatcactct cttggaatga ctggtgttat tttgatggta
    30061 gcaggcgggc tttcttacac gataggcgca ttcttttata gcttgaattc tatccgctat
    30121 atgcatgttg tttggcattt gtttgttatg ttaggtgcag gttttatgta cttctctgtt
    30181 ttattcttta cttaataaaa aaaataaagc tatggatggg acagctctaa ataacagcta
    30241 gtctatccat agctttttta tatcgtcaat tcgcctgtcc cgctttttac aggattctca
    30301 ttacgctgat acgtttcaaa ttgatgcgga taacgatttt tttcatccat ttctccttga
    30361 ctagtattga tcagtgacca atcagaccag tcaacaggtg gaaaataagc atctccatcg
    30421 aatacatgat ggatgactgt acggtataat atcgtacaaa atggcagaaa ttcttgataa
    30481 acagctgacc ctccagcgat gaaaaagata ccgtctgttt ctttttccag agcaagcacg
    30541 tcttccacac tatgcatgac atgcacgcct tctgctttat aggaagaatc tctcgtcaaa
    30601 acaatcgtct gacgattcgg caacggacgg ctacccatcc cttcgaatgt ttttcgtccc
    30661 atcactagtg tatttgcttc tgtcatttgt ttgaagaatt tcagatcatt tggcaaacgc
    30721 caaggaagac ggtcttcttt tccaatcaat ccgttttcat cctgtgccca aattgcagct
    30781 aacattttct catcctttct gattctaaac agcgatagga gctttgatcg ccggatgtgg
    30841 gtcatatcct tctaattgga tatcttccat ctcaaaatca aatactgact gcttttcctg
    30901 attcaaaact aaagtcggaa atgcgcgtat ttctcggctc aattgttctt tcatttgttc
    30961 gatatgattg atataaagat gagcatctcc taatgtgtga acaaattcgc caaccgctaa
    31021 acctgtttca tgtgcaatta ggtgagtcag caacgcataa cttgcaatgt tgaacggaac
    31081 gcctagaaac acatccccac tgcgttgata caattggcag cttagccgtc cctcttgaac
    31141 ataaaattga aacatcgtat gacaaggagg caaagccatg ctaggaacat cttccggatt
    31201 ccatgcagaa acgatcaatc ttctagaatc aggagtcttt ttgattgctt cgatcacttg
    31261 tttgatttga tcgataaagc caccgtcttt tgtttcccag tgacgccatt gatagccgta
    31321 gatgtgtcca agatcgccgt atgtttcagc aaaggaatca tcggttagga tcttctcgca
    31381 aaaaagcttg ctctgctttt tatattcttc attgaattca ggatctgtca aacatctatg
    31441 gccaaaatca gtcatatctg gtccattata gtctggactt ttgatgtagc gttcaaatgc
    31501 ccattcatcc caaatatggt ttttattttg taatagatag cggatattcg tatctccttt
    31561 taaaaaccaa agcaattcgc ttttgatcaa tccaaaaggt actcgtttcg ttgttaacaa
    31621 aggaaaccct ttttgcagat caaaccgcat ttggtatccg aataagctta atgtgcctgt
    31681 gcctgttctg tcttctttac gatgcccttc ttccaacaat tttcttccta agtcaagata
    31741 cgcttgttcc atcctttttc ctcctaatct tctacccatt cactcaagta ttcccaccgt
    31801 gccatttttt cttcaagttg tgcttctgtt tcagatacgt cgttttgcaa ttcttgtaat
    31861 cgagtgaaat tatctccttg atgattcatc tcttcttgga gcaatgagat tttttcttca
    31921 agttctgcaa tctcatcttc aatcgtttcc cattcttttt gttccatgta agataatttc
    31981 ttcttttcag caggttcctt gctctgtatt tttggtttcg ctttttcagc aggctgctgg
    32041 gtctcttttt gcttcagtaa ataactggac atgtttccgt aataaagttc ttgttttccg
    32101 tttccttgga atactaataa cttatctgct accttgtcca agaaataacg gtcatgggaa
    32161 acagtgatca cagcacctgg aaaacttcta aaataatctt ccaaaatcgt caatgttgct
    32221 atgtccaaat cattggtcgg ttcatccaat aataaaacat taggttgctg aatcagcagt
    32281 ttcaacaaaa ataaccggcg tttttctccg cctgaaagtt tgcctatcag tgtcccatgc
    32341 ataaatcttg gaaataaaaa tcgttcaagt aattctgcta ctccgatttg actgccatct
    32401 gctgtttttg cttcttcagc agcttcttgt aagtaagcaa tcatccgttt atctggtgcc
    32461 atttcttcat tttcttgtgt ataatacgca agatgaaccg tttcaccgat agacattgta
    32521 ccgctatcca ggggaattct gccagctaga atattaagca atgtcgattt tcccgcacca
    32581 tttttaccag taatacctag tctttcccga gattgaatca ataaatctaa atgttctagc
    32641 agtgtttggt catttatacg gtaactgcca tctttgattt ccagtacttt tttccctaaa
    32701 cgttgtgtcg ctaggttcag ctctaaatca tcttcttggt ttacttggta caagttctcc
    32761 tttagatctt cgaaacggtt gatccgtgct tgctgcttgg tgccacgtgc ctgtacgcca
    32821 gccctcatcc aagctaattc ctgtttataa agccgcttgc gcttttcttc ttgttctact
    32881 tctacacgat cacgttctgc tttttctaag acgtaagctt cataatttcc tttatactcg
    32941 tataattttc caaatgaaag ttcgaagatt cggtttgcta ctctatctag aaaataacga
    33001 tcgtgggtaa ccatcaatag tgcgccacgg tattgtttca aatagttttc caaccactcg
    33061 atcgcttcat aatctaaatg gtttgttggt tcgtctagta atagtaaatc gggttcatca
    33121 atcaacactt gcgccagact aactcgtttt ttctgaccac ctgataaagt actgattttt
    33181 ttatctaatt cactgattcc taatttttgc aaaataatct ttgcgttggt atctgtagtc
    33241 caagcatctt ctttattcat ttgttcttct gctttagcat aacgtttttg cacatcaggg
    33301 tcttctccgt tttcccctag ttcgatcaat gcttcctcat atgctcgaac agtttggatc
    33361 aaggggctgt ttccttgaaa gactgcttgg agaacagtta attcatcgga aaattcagct
    33421 gcttgagaaa gataaccaat acggtaatca gttggataaa acactgtttg ccgatcaccg
    33481 tcgctgctat cgataccggc aataatattt aagagacttg ttttacccgt cccgttcgtc
    33541 ccaatcaacc cgatacggtc tttttcgtgg accaaaaaag aaatctggtc aaacaaatct
    33601 ttttcaccat aagttttttt gaggtcagtt acttttaatt ctttcaacat gttccctcct
    33661 tatcttggat attgtatcat aaaaatagtg aacttcagga aaattttttt ggtatttgtt
    33721 ctttttgtgt tctcaaaaaa agaacaggac cgattcgatc ctgttctgat atgttttgtt
    33781 ttttataaaa cttgaaaatt tttacctttt tattttattg gactcgtttt ttttgacttt
    33841 gctcgaaacg caaatcggaa agaaaagtta tcagatggtt tgttcatcgt ataagattct
    33901 aacaaattga tattcctgcg atagctgttc gcagaaaacg tcgtgatttt ttcagcaact
    33961 tcgtattcat caatgatttt tctttcaaga taataacctc ttagcatttt tttcgtaaac
    34021 agactatctt gctggatcat ccacgaagaa atcaaattcc ccgagttcat ttttattcct
    34081 tcaagtttgc gttgctggat aaaaaattga accatcgttt catcataaat atcagtcaag
    34141 ttatctaaac tttcaataat caactgagta tttttttgat atgtctctct gatttcttgg
    34201 atatcttcat cactaataaa tgtctgacga tcagcatatc ttctttgcca aaacattttc
    34261 ggatggagaa cgattcgcat taacctacgg acaacaatga accaaaagcc gataaaagac
    34321 aaaatctggc ttgtgataga atgttcgaat cgagaaagaa aacgggagta aaaacgataa
    34381 ccattagaag aaagcttgcc cgaacgataa ctttcatcca atccatcccg ttcgatcgac
    34441 aaaatcagtg cttgtatttc ttgtacttct tgcttttcat catcggttaa atcttccaag
    34501 tacagatcac gtatccgttc ttgataattt tctatcactg cttcagtggc taaaaattct
    34561 tttgtattac gatcaatttc ctttatttct tttcgcaaaa cttcaacgac ctcttctaaa
    34621 atcatcaaag cattcaaatc cgtgcttttc gcttcctctg tttccgtcag cataggcaag
    34681 acaatgatcc ctatgaccaa agtcactaag atgacacccg ccgtcagaaa caaaagcaaa
    34741 gaccgctggt aaaacatcat attattgatt gaaaatggca aaataaaaat cgtagccaaa
    34801 ctgactgttc cctttacacc gccaaatgtt aagatcaaga tctcgttcaa ctgttttttg
    34861 aactttttac tgccatcttt gaatacataa aaaagactga tagaaatgaa tctgatcaca
    34921 aaaagcatga cagagattaa tacgataatc gctaataaaa gaccgtttgg atataaaccg
    34981 tctccccata ctggcgagaa cacttgcgtc aattcgattc ctaaaaatat aaatactaat
    35041 gcattcagtg taaaagcaat agttgtccat gtactgtggg acaagctcga caattctgca
    35101 tcaaacacag agattttcct aaaaccagaa gcttggagaa tacctgctgc cactgcagca
    35161 atgattccag atacatcaaa tgcctctgcc aatacatagg ctagaaaagg aagcaaaagt
    35221 tcgatcagca gataccctgt gacatcttgt gctgatgctt tttcgatcaa atgaattatt
    35281 ttttgtttga accagacaag caaaaagccg atcaaagctc ccccaatact agaaactact
    35341 agactcatcc ccgcgtttac tgctgaaaaa gatccggtga tcagcgcacc aagagcaaac
    35401 tggaaggcag taacaccaga tgcatcattc aaaagacctt ctccttccaa aatatgcatc
    35461 gcctttttag ggatattcac tcgtccagaa agagaactga ctgctaccgc atctgtcgga
    35521 cctaacgctg ctccaaaagc aaagcaggca gcaagtggga cacttggcaa caggaatgat
    35581 aacgtcgcac ctaccccgac aagggtcaaa atgactcctc caaatgccaa gaacaaaatt
    35641 gtcccgaaat ttttgagaat cgaagaaata tctgcttttt ctccttctct aaataacaat
    35701 ggtgcaataa tcataactaa gaacaattca ggttcaaaat cgattgattg tccccactct
    35761 gtcagaccta ataccactcc aaggaaaatt tggatcagtg gagctggaat cattggcaaa
    35821 atgcggctag ctatgttcga taaggtgatc gttactgcaa atacaataac taactcagca
    35881 aattccatcg tccatcccct ttatttttta ttttgtgtaa gcatttttaa aaattgttct
    35941 tttttcttta aaattttggt ttcgactact tcaacctgtg tttggttccc ctctaattca
    36001 cactgtacac gttttgactc caaacgaagc agatcatgtt tcagacgttc caactgacga
    36061 tacatcaata tatttctttt acgcaactta caaagtattt gacggcggtc atccatcgaa
    36121 agggcgccat agcgatcatg caacagtttt ttttgttgtt caaaactctc tctcattttc
    36181 cagatctcct atacacaaac ttccatcatt atagcaaagt tctttttgga tgaaaaatat
    36241 ttcattgttt catcgtttat tgctagttag aaatttccat aaatttttca acgcctcatt
    36301 ctattccaaa tagtatcaga tatcaagtaa aacttaagaa agataaaagt ttctgacaaa
    36361 aggatgttta ctttcccatt cctgcctttc ttgttctttt atcacaacaa aaaaatgggg
    36421 ctgcgacaaa aatcttgtca cagtccctta ttctgaataa acggtgtgga agagacagcc
    36481 tatcagtagt aataagacac atctctttca cagcctcatt tttgcgtaaa aatcgattta
    36541 ttcgaaaaat tccccgtata catcttctac acgttgatct ggattgatca cgatatacaa
    36601 gttacctgtt ttttcgctga tttttgttga ttgatattga ttgtctaatc cttctgttcc
    36661 tttatctaga tatgggaagt acacaagatc aggtgtgcca tttcggtaaa ggatttccat
    36721 tcgctcgata tcttcgtatt tcaatgcgcg attaaacatg cccatttcca gtccgcctag
    36781 gttgatatca tgtgtcgcaa ctgcgtcccc ttcatggaag atttcgattt taaatccagc
    36841 acacggatgg atttcaacaa attcactgcc attgattcgt ccaaaacttg ttgtgatttg
    36901 tttgatccaa agatcgccga tatgacggcg gttgatcgtc caggtttctc catttctaaa
    36961 ataaaatttt agcgctgtca tttctttact cattaaaaag actcctttat gatgtaattt
    37021 attcttgtac tacctcagtt tagcacaaat ttatgtaagt ggtcacatct tatgtttttt
    37081 cttctaatga cgtatctgcc cactttaagt agccaacaaa gctgagcaga tacagtctag
    37141 tgttttttaa atcaattttt ctttcgataa aaaatccaaa acctgttttt tatcattttt
    37201 tattctttgt tctacgacgg ctttttcagc taattgcaaa gcttcactaa cccacttacc
    37261 aggttttttt ccagtttctt ctagcaagat tttaccatct actgctaatt cgcttaatga
    37321 cttgataggt aaacgatcat aggattctcc tacggcatcc atctgaggtg tttcgtcaaa
    37381 atagatcatc aattcttcta cttgcagtgc atgttttttg cctaagaagt acaattcgaa
    37441 tggttccaaa gctccagttt ctcgttgtcg tagtccttgg acaagtgctt gaacgtcttt
    37501 aatcatttgg ttagaacact tccacgattt caaaaaggaa cgaatctcat tttcttttag
    37561 atccagttgg ctgatcaata atgcccatgc ttggctctct tctttcaatg ggacggatgg
    37621 taattcagca aaacgtaaaa gtgcttcgcc ttcttctttc aaaccgggac agcaaatgta
    37681 gcactctgtt tcaataaatg catggattcc cgcggcccgt tcatttccca agagcatctt
    37741 gatgaattcc acatttatac gttctacaga tattttacct aatagctcgt ggttttcttt
    37801 gatggcgtag aaggtttctt cctctaattt gaaccctagt tgactgacaa aacgcaaccc
    37861 gcgcatcatt cgtaaggcat cttcatggaa ccgctcatga ggattgccta cagctcgcaa
    37921 gatctgttct tctagatctt gcagaccatc gaacaaatcg atgatttctc catcttcttt
    37981 caaagcaaac gcattgatcg taaaatctct tcttttcaaa tcttctgcca acgaacgaac
    38041 gaattcgaca tgatctggtc tacggtagtc ctgataagtt gattccgtcc gaaatgtggt
    38101 gatctcatat tgttcatcat tttctaatac caacacagtt ccatgttcga tcccaatatc
    38161 tatcgttttt ggaaaaagct gtttgatttc ttctggaaat gcgcttgttg cgatatccac
    38221 atcatggatt ggctggttca gcaagatatc acgaacactt ccacctacaa aataagcttc
    38281 atatcctgca ttctctaatt ttttcagaac tggaattgcc ttttggtatt ccagaggtaa
    38341 ttgttttaat ttcatttttc caacctttcc tgcgtgtttc ttgtcctgag ataaagactc
    38401 agttgcatgg tgactgtttg acaagtccgt ttcagagtct tcaaggccag gaccacttca
    38461 tcttgtttac tcgtccttac gctcaaagcg attatggtct ctttgattga atttttccat
    38521 gtttttttca aaaacttcag ttaagtcgat attcaatgaa ttagccatga tcatcgtaac
    38581 gaataaaaca tcaccaagtt cttctgctac cgagttaact ggttcagaag cctttttgct
    38641 tttttctcca taagtgtggt tgacttctct tgccaattca cctacttctt cagttaatcg
    38701 agccatctga gctaatggag agaaataccc caccttgaat tgctggatat attcatccac
    38761 ttcctgctgc atactgtata aagaacgctt tttatccatc tttttccctc caaaactatc
    38821 ttaccaaaac tttaacgcaa atcctagata agagcaattg agaaaatcga atttccatgc
    38881 tacatttagg aaggaatttc taaatggaaa ttctgtgaaa atacataatt gaaagctgga
    38941 attcatatgg tcaaacgaaa aacagtaaaa gaagttcttt ttatcattat aggaacaagt
    39001 atttatgcat ttggtcttgt ttacttgaac atcgctaatc atttagcaga agggggagtc
    39061 tcagggatca ccttgatctt aagagcttta tttggtattg atccagctta tacgacttta
    39121 ttaatcaaca tcccattgat tttactagga ggaaagatcc tcggtaaacg ttccttggcc
    39181 tatactgttc taggtacact ttctttgtca ttctttttat ggatctggca aagggttcca
    39241 ctagaaatca atttacagca tgatctactg atcgtttctc tacttgcggg tttagttgca
    39301 ggtgtaggaa gcgggatcgt ctaccgcatg ggtggaacaa caggcggcag tgatattatc
    39361 gctcggattt tagaacaaaa ttacggcatc actatggggc gttccctatt ggcatttgat
    39421 atcatcgtct tacttgcttc attaacctat atcgacttga aacgtatgat gtatacgttg
    39481 attgtttcgt atgttttcag tcgtgtgatt gattcgatct tagatggcgg atattctgca
    39541 aaaggtctac ttgtcgtctc caacaaaagc gaagagattg caccactact gatgaccggt
    39601 ctgcaaagag gtgtgacttt cctttctgga gaaggcgcat tctctggtaa ttcaaaaaaa
    39661 atcatctata tggtcgttag ctctagtgaa ttaactgaag ctaagaggat cgtccacgag
    39721 attgacgaaa aagctttcct ttcaatcatc aatgtgcatg aagtggaagg gcaaggcttt
    39781 acttatttga agccacagac aagattactg aaaaaactat aagaaaaaag acaaagatag
    39841 gctatgagga aagattccac atccagctga cctttgtctt ttttagtact atactatttt
    39901 taactaattg ttatgttcac ttacccggtt acttttcttt tgtgtctgca aaaggatctt
    39961 tcggttaact tcatcagata atttcaaaaa agcatcccta tctccatctt ctagtgcttg
    40021 atcgatttga gcataaagca tttttagttt tgcctcttct tctttttctt gaaaatatcg
    40081 ttcaattaat tcagtatctt cttcactgac tgaagaatcc caagaagaaa atggattgtc
    40141 ttctaagata gatagatatt ctcttgtctg ccaagcattt tcgaatatac attccacata
    40201 taaaggcttt ttccaattca aacggatttc atgaaaaatc tggtctgtgt ccgtaaattc
    40261 ttttccctgc aaaaacaatt tcatgggttc cccttcaaca gatagatcac tgatctgcag
    40321 tcctcgatcc gcaaaattcg ccccttcaac aaagtggaca ttgcttaaaa tcgcttcatg
    40381 attggcaaga taattcaaga tccaaaaaac ttctcttcgg ctaaaagata catgattgac
    40441 catccacgtt aaaaaattct tcttttcccg tacatcaatt agcatttttc tcacctctct
    40501 gttaggtcat ctaataatga cagtgcctcc atatctcctg gttcaagtgt taaataataa
    40561 gtcaataatt cttgcgctct tttcagctgc ccttcttctc ttaagaaaaa ggcatactct
    40621 ttcaagaaat ctggttcatg tgatagttca tgataagcct gttcatagtg tacagctgca
    40681 acggtaaagt cttctaattc attataagca tgggctaggt tccattctgc ataaggatta
    40741 gcagtttctt ccatctgatt gatcacttgt atcacatctt catatctttc atcatccaag
    40801 taaagattac ttaaagtaag taatgtttca tcttgtttgt ctcctacttc taaagctttc
    40861 agtaaaagtt cttctgctcg ctgtctatca tgcagacgga aagcattttc cgaggccagg
    40921 tgataaagtt cgacttggta aggattttct ttgattcctt cttctaacac tgtttgtgct
    40981 tcttcaatca tttcttcttc ttgaagagct tgtcctagat atagatataa cgattgataa
    41041 tgcggattaa cttctcgaag ttcttccaga tagttgattg cctgctgatt ttctttcaat
    41101 tgaagattcg taaaagctag ctggaacagt cgatcatctg tcctttcttc tttcagtgct
    41161 tcttctaaag gtacgatagc tgcttcgaat tctccttgca tacttaagga ttgtcctaaa
    41221 cgttcctgca tcgagattcc tgaaatttca tttatcccgc ttgccaacaa tgtttcataa
    41281 accactgctg cctctttgaa acgatcaact gaaaaataca attcagctag cgcaaactgg
    41341 atcaatggtt catcaggaag caacccagct gcttctttca attttgcttc actcacttca
    41401 gggattccga taacttggta caaatcagct atcgccaata agctttgcgg atagtaatca
    41461 ctttgcttat ccactttttc caggtattca aaagctaagt cgatctcatt atcttcgatt
    41521 gcaatttctg ctaacgggat gttcagtccg tcattttctg ggtattggtt gatcagttgt
    41581 tcaaaaatct gttttgcttc gatcaaaaag ccaatagcca gcaattcttc gcctagttca
    41641 gctaatatat ctgctgggtc ttcttttaat gctttttcta aaaagatttg tgcttgtgac
    41701 aagtcttcat ctgataaagc ttgcaacatt ttttcgctat ttgtattcat ttcaattccc
    41761 tcttatctca ctgacgattt gttgaaatac cgtcattgtt ctcaataatt ctttttcgtt
    41821 tggatgttgg tatccgtcta ttatacctat tttttctaaa acaagtattt ttttgctttt
    41881 ttctgccaaa aaaagaactt tttccaaata ctccatcttt gaaatctgtt ccagccagtg
    41941 gattggatag ccaagcaagt ccagccacac aaaaaagttt tttagttgga aatcaaaagg
    42001 atatgcttgg agattccaca tgaaatgaaa catcattcct aaaaattttt tcatactagc
    42061 agaaagtaga tggccatttt cggtcagata aaaagcttgt tcaaacagtt taccataatt
    42121 tgacaggttt tctgcctcta attgataaaa atagatcatt tcttcgatca tcccggcaaa
    42181 cggccgagtc gttaattgct ttttatttgg atagttttta tacagatttt gcaaaaaacg
    42241 atgatcacaa atcattccgc aacagataaa agtcaaaaag tcaatctgct ttccgtccac
    42301 ctgccgctcg ctgattgttt gatcataaac gatgcgttct ggcagattga tggtttgcag
    42361 cactacacga tttggtagct gcaaaatcgt ccgctgcggt gttaatgctc ggcttagaga
    42421 acggatcgat acagggatca tccatagtga tgaatcaagg gttgtatgtt tttgaaaaaa
    42481 acctgccaga tccatgatcc cttcatttcc aaatccgatg ataaacatgg attgattttc
    42541 gggatagcgt ttgacgaaat caagcaggtt ctttaattct gttaaatgat tgcattgagt
    42601 gttggcacaa atataccagt cattgtttgc ttgatttgca aataacgggt tgatcttatc
    42661 tgcaaacaaa tcataatagc gctgattcgt taaaaataag attggctgat caatggaaag
    42721 ttctcttagc tgactgacta gcgtttctcc gtaaaacact ttcgtcaacg catcttctac
    42781 ttgataatca agttccatac gcatccctcc tcttcttatt tttattttac cattagtaag
    42841 caaattctag aaaagataag agcaaagctc acctcataac ttgcaaataa ataaaaatta
    42901 aataaaatac atgtgttttc tatgtatgac atacggattc ggaatctctg tctcttattt
    42961 attccttttc tgctaggaaa taaaaagaaa agcacttgta gccctagagc cacaagcgct
    43021 ttttcaactg tcttatttaa cagcgtcttt taatgcttta cctggtttga atgcaggtac
    43081 tttgctagct ggaatttcga tttcttcacc agtttgtggg ttacgacctt tgcgagctgc
    43141 gcgttcgcga acttcaaagt taccaaaacc gattaattga actttttcgc cgttagctaa
    43201 agcatcttga atagttgaaa atacagcatc tactgctgca gttgcatctt ttttagttaa
    43261 atcagtagct gaagcaactt tttcgattaa ttctgctttg tttgccatgg atagattcac
    43321 ctcctccaca agagatatga tagaaatatc atttagttaa aactttctga gtggtccaga
    43381 agtttaaaaa gaaatattga ttaacttcaa tattctgtta tcacgatatc atatgaaccg
    43441 cttaatagca aggtttttac caattttcac atgctttttt tagtcataga aacgtaacaa
    43501 acgaaactgt ctttttcttt tgaaaaaaaa acgtcgatat ggtaaaatgc ctattttctc
    43561 ctacgaggga taattcggat ggacgtccct tcaaaagtaa atgcttttct gatttgattt
    43621 tctaagaaac gttcataaga aaaatgcatc aattcctctt cattgacaaa aacgacaaat
    43681 gtaggtggtt tgacagcgac ttgtgtagca taaaaaatct tcaggcgttt tcctttatct
    43741 gttggtgttg gattgattgc tacagcatcc ataatgatat cattcaacac agccgaagag
    43801 atacgaagat tctggttcat acttacttgt tcgatcattt caggcagacg ttccaaccgt
    43861 tgtttagtca ctgcagaaac aaaaacgatt ggagcgtaat caagataccg gaattcatca
    43921 cgaatctctt gttcgaaatc acgcattgta ttggtttctt tctttacaag atcccattta
    43981 ttcacgacga taataattcc acgaccagct tcatgggcgt aacccgctac tcgcttgtct
    44041 tgttcccgaa tcccttcttc agcattcagt accatcaaaa cgatatcaga acggtcaatg
    44101 gcacgcattg cacgcatcac gctgtatttt tcagtatttt cgtatacttt tccgcgttta
    44161 cgcataccag ctgtatctat cattaagaat ttctgaccac tttcagaaac gaagtgggtg
    44221 tcgattgcgt ctcgggtcgt tccttcaata tccgaaacaa ttacacgttc ttctcctaaa
    44281 atcgcattga tcaaagagga tttaccaaca tttgggcgac caatcaaact aaacttaatc
    44341 gtgtcgtcgt cctcttcttc agtctcttca gagaaatact tcactgcttc atctaaaata
    44401 tcaccaatcc ctagtccatg gcttcctgaa acaggataag gatcgcctag ccctaaggaa
    44461 taaaattcat agatatcgtt tctcatttct ggattatcta ctttattgac tgctaaaatc
    44521 actggtttat tacttctgta taaaattttc gcaaccatct catctgcgtc tgtcacgcct
    44581 tcgcgtccgc tgactacaca aatgattaca tctgcttctt caatcgcgat ttctgcttga
    44641 tgcttgatct gatccataaa aggttcatcg ccaagatcaa ttccgcctgt gtcgatcacg
    44701 ctgaattctc gacctaacca ttcacctttt gcataaatac gatcacgagt tactccagga
    44761 gtatcttcga caatcgagat acgctcacca gcgatgcggt taaaaatcgt agacttaccg
    44821 acgttcgggc ggccaacgat tgcaattgtt ggatttgcca tattatttcc tcctcacaag
    44881 tttccaatct cacttagttt aacgaatgca ttgccaactt tcaagcatag attgtcaatt
    44941 aaacgaagta tttttattcc ttattgaaat actaatcgct tttcaaaaga aaaactgaga
    45001 caaaagcagc tgctttcatc tcagtcacag atacaaaatc aattattttt cagaatcatc
    45061 ggttgctttt tcttctactt cttctttcaa cgcatcacct aaaatgtctc ccatagtaaa
    45121 tccagtattt tcttctggta attcataaga ctcaacattt tttggttctt cttctgaagc
    45181 tggtttttct tctaaagctt tgatactcaa tgcaatacga tgttcttctg gatggatctc
    45241 taatactttc actttcactt cgtcaccttc atgaagcact tcatgtggtg tcgcaatatg
    45301 tttatgtgaa atttgagaga tatggactag cccttctact ccagggaaca cttcaacaaa
    45361 ggcaccgaag cttgtcaaac gtttaactgt tccgtctaat actgtgccaa ctggagcttt
    45421 tgtatcgatt tctgaccaag gtccaggctg tgtgtctttg attgacaagg aaacacgttc
    45481 ttgttcagga ttgatagaca acactttgac atttacatca tcattgacag ccaatacatc
    45541 acttggtttt gctacatgac tatgtgaaat ctctgagaca tggactagac cgtcaattcc
    45601 gcctaggtcg acaaatgcgc caaaatcagt aagacgtgca acacgtccac tgagaacatc
    45661 tccctcatgg atagttgaaa gaatttcttt tttcttttct tctctttcgt tttccactac
    45721 cgctttatgc gataagatca aacgattttc tgatggttcg atttcgatga ttttaaagct
    45781 caatgttttg cctttatagt ctgaaaagtc tgcaacaaaa tgatcttcca ccattgatgc
    45841 agggacaaaa ccacgtacac cgacgtcaac gactaaaccg cctttgacaa catttgtaac
    45901 aggtgcttcg atgaccttgc cctcttggaa atctctttcg atatcttccc aaactttttt
    45961 agcatctaaa cggcgttttg aaagtagata gctgccgttt tctttatctt tgccgatcgt
    46021 actgataacg actaaatcta atacatcacc gacttttact acttcgctga tatcttctac
    46081 cggcaaagtt gataattctt ttgccggaac gactccttca actccagccc cttcgattcc
    46141 gacaatcact tgtttgtctt caatgactaa aacttcgccc ttaacaacat cgccaacttt
    46201 tacttcttgt acgcttttca ttgcgtcttc cattgactgg ttgttctctt gattttgatc
    46261 aaattctgtc ataacacttg tcctcctaac caacattctg ccgtaagtac ggtactgttt
    46321 ttagtttaat tgattattca aaaaaaataa agcgatttcg ttcgttttcc ttgaaaaaac
    46381 aattgttttt catttttttt cttaatttaa aaaagaagat aacctttttt caaaacaaca
    46441 gcttttattg ctgcaacgac ttctggaatc gatttgccag tcgtatcgat acgaacagca
    46501 tcctctgctt gcttcagcgg agagacgtcc cttgttgaat cataatggtc tctttcctgt
    46561 atctctttcg tcagtgtttc aagatcagtc ggaattcctt tttcttggtt ctctttgaaa
    46621 cgtctttctg cacgctcact tacgcttgct actaaaaaga ttttcacttc tgcattcggt
    46681 aatacagctg tcccgatgtc tcttccgtcc atgacaacgc caccgcgttc ccctattttt
    46741 cgttggacag caactaattc ttctctgact tttccatgtg cagagacttc agaaacagct
    46801 tttgtgacat ctggttgacg gatttctaaa gtcacatctt ttgatgctat gaatacatgc
    46861 tgtccatctt cttgttgcga aaaagagata ggatactttt tgatcagcga aactagccct
    46921 tcttcatctg aaaaagatac attgttcgta atagctaaat aagttactgc gcgatacatg
    46981 gcaccggtat ctgtataaat ataattcaat tcttttgcta atatcttggc aacggtactt
    47041 ttcccagatg aagcaggccc gtcgattgca atactgattt gtgtcatgat ataactcctt
    47101 tttctcagaa aaaaagccgc aaagcgcggc tattattatt taattcttaa ttcttgacca
    47161 gggtaaagca taaagttgtt tggatctaag ccattcaatt caaacaattg atcagtggtg
    47221 atccctgcac gctcagccac ttgttttgga ccttcgcctg cttgaacagt cgttgttgaa
    47281 ccggctgcag ctgatgaaga aggtgttgtt tctgctgcat tgctgcttga atcccctgct
    47341 tgataagttg cctcacttga ggaagtcggt gttgtctctg ctgcaatact gctttctgac
    47401 gttacacctg tatcttgaga cacagtagat tcttccgcga ttgtactaga ttctactgtt
    47461 gaagtctcag ttgactcctc ttttgttgta cttgtagaac tttctactac tgatgtcgaa
    47521 gtttcagaag aagttgctgc tgttgcattg ttgcttgatc catttctgat ccagaaataa
    47581 gcacctgctg gaatagcgat acataagacc agtaaaatca aaaatagcga aagaaataat
    47641 gtatttcctt tcttttgttg tctctgagaa cttcttgatg aaacttcatc atctgtatcg
    47701 tagattggtt gttcccatgg ttcttgtgtt tcgttatttt ctgtagattg atgtctatct
    47761 tttctgctca ctgttttacc tcctaaactc tatcgtgtgt attataccat aggaaaaagt
    47821 gtcttgtcct aaaaaatcaa tgttccatgt tttttttaaa taaatttagt aaaatcgttt
    47881 cccaatgaag ttgttcagtc ttagaaaaaa gcttcctctt tggcagaagt tctgcttttg
    47941 ctccatcaat atcacaacaa ttctgcggct tttcggagag tttttcgcca aaataagcta
    48001 agatgaattt tctgcggcag ttttcttcat taatatagcg aagcatctgc tgcaggcgga
    48061 attctttttc tttttcctga cgtttgagcc gttccaccca attttctggt ttttcagact
    48121 gctggatcag ctccatccat tttttttgca gctcatcaaa gggagcttgc tttgtggcat
    48181 attccaaata aagctcaaaa ctttgtcgct gttctctaga aagttgatta aagaaataat
    48241 ggatacgttc atccccactt tgataaaaaa gaattgctgc actttcttcc tgatctctgc
    48301 ctgctcgacc gatttcttgt acatagtttt ctagactgtc tggcaaatca taatggacca
    48361 cataacggat atcagattta tcgatcccca taccaaatgc attcgtagca atgagaaact
    48421 gcagctgatt ttttacaaac tgctgttgaa gttgtcttct ttgagctgca tcaagaccac
    48481 cgtgatagta tccaactgta aaacgttccc tgaataaatg atacaatcgt tcgacttctt
    48541 tttttgttgc acaataaatg attgccgctc cttgtgctct ttccatgaag tgttcaagtt
    48601 cttgttcttt ttgctgcgtt ttccgaacaa aaagcgagat attttgccga ttgactgatt
    48661 gtctgatctc ttttggtggt cgatcaaata gtacatgacg tatatctctt gctactaaat
    48721 ctgttgctgt cgccgttagt gccagtgtga ccgggtttcc taaaagcttt cggatttttc
    48781 ctaactgttg atattctgga cggaaatcaa ctccccattg agacacgcaa tgagcctcat
    48841 ctactacata taacgcaata ttttgttttt tcagatgctc taaaagagaa ggattagtca
    48901 acatttcggg actcaaaaac aaaaatttat attgcgatag atgacggagg acgtatcttc
    48961 tttcagattg cgaaagtaaa ctattgaaag cagctactcg tttctcccct cgtttttgta
    49021 attgcgtcac ttgatcttcc attagagaaa gcagcggtga cacgatgatg accagacctt
    49081 ccattaaata ccccgtcaat tgatagcaaa ggctttttcc cgttcctgta ggcaaaatag
    49141 cgagagtatc ttctccatca agcaacgcct gaataatttc ttcttggccg ggacgaaatg
    49201 aatcaaatcc aaaatatttt tttaattcat ttattaacat tctgtatcgc ttcctttctc
    49261 aatagataga tttgatacaa acgaaattct ccataatcta attgtccatc tacattccat
    49321 tcgctaaagc gccactctcg cacggaatcc ttatttggtt ccaaacgttc gattgtttct
    49381 gcagagagaa tccactcgta tggaaaatca tcaaaaaaca aggcccactc aataagatga
    49441 tctgttactg ttcctctttt taaatgacgg attgccatga tctggtcaat tgtctgacca
    49501 ttcataaaca tctgcttggt gatcatcatg ctttgattga aattttgctg taataatgaa
    49561 tcgatcaaag cgtgccaaag cgaatctggc cgtttttcga tttgtgctaa caaaaggtgg
    49621 atacatctgc tttgttgcag ataaacctct gactcatcat tgttttttgc cagctgataa
    49681 ggaagcagcc ctgttcgctc atttccagat aattggttag ctaaaaaatc tgctgcttct
    49741 tctggtatct ccgaaaacag ctgcaccaaa ctctcatacg cagtatcaat taaaatttca
    49801 cgtggcaatt ggctttcact aagccatttt tttagttgga aagtataaaa aggacttgtc
    49861 tctgctggaa gatagtcttg aatacccgtt gccagattag aaattacttg aacagcaaat
    49921 ttaatgagcc gccaactagt ttcccctgtt ctgccatagc ggtcaaaacg caaaccagta
    49981 tgttctattt gatgttcgga caacatatca gcccctgcag gcgttagctt tgcttcattt
    50041 tcatttattt cgatccaacc ttgctgcgca attttttgca aaatttgata aaacttatcc
    50101 tgcttcaatt ctggaaaact gttatggata aacaaaagtt cgtgggtaaa tccataaata
    50161 agtactgaag tcgttcgttt tcccacaagg agttggtaaa gagaactaat ccgcagcttg
    50221 tgtcctgatg aaaatagcga taaaataaaa acaatcatat tattctcctt aagttaggat
    50281 ggttagaaat gtcactacta tgtaaacttg tacctgaacg ctgtattgca tgcggtcttt
    50341 gtcaaatcga agcgccagat attttcgatt atgatgaaga aggaatcgtt ttatttgccc
    50401 aagaaccaga ggctcatcaa cagttcgtga tgccggaaga agaaacatat gtaaaaaaag
    50461 cgtatcaaaa atgtccagtt cgagcgattt tattagaaaa taaaatataa atataaacga
    50521 aactttaaaa acgtacacta agttctctaa acttgaaaat aagccgggat ctgtttctct
    50581 tgttccgtga aatcccgact tattttgaaa gattcatttg ctatctatag ttaattgact
    50641 agttttttca cggaccctat aggacccatt tatattattt caactttttc agccagtttt
    50701 ccaatgtatt gacgatcaca gtttgctggt ctgcgttaga aatttctgcc tctccatctc
    50761 ctttttgctc accatagctg ccaaaacctc catgatttcc gccattgatt gatacatact
    50821 ctgttctttc cggtaaatac tgcttagctt cttgataagc ttcctgattc aatactttat
    50881 cttttgtcgc cgtgatcgat aaaacaggaa cagctgattc tgtcaatgtt ccttttttgt
    50941 cgggataact tgccatgaag aatacccctt ttaaatgttt gtctgaatga ttcttattga
    51001 cataacgact tgccattacg ccacctaatg aatggccacc gatcacataa tcggaatttg
    51061 gaaaatcttc tttgacctta tccgctttgt ttccgcccat cactgctaga tcaagtggca
    51121 ttttgacaat agcgactgaa aagccgtttt ttgctaaatc ttgcgcccaa atactataac
    51181 tttctggctc tacgagagca ccaggataaa aaataagtaa tggagacttt ccatgattct
    51241 ttttctctcc ttcaaaaaat aagtaatctt ctttgttttt tccttctttt accaacactt
    51301 gcgcttctga cgatggttcg tacgtatttt gctgtatata gtaataaccg gctccaccga
    51361 gtaatcctaa aatgatgact aagccaatta gtattcgtct aaatattttc actttctccc
    51421 accttttcta tctgagtgac ccctttttct taatcaagta tgattatacc ttaaaaaaat
    51481 aagagagaaa tgcaaacttt ccagcatcaa ataaaaaaag acggaatatc catttgaata
    51541 tccgcccttt ttgtatgttt atttttattt agcacataaa cgatacacag cttctgcata
    51601 aatttccatt gctttataca tactacttaa ttcccatcgt tcattcgcct ggtgcataaa
    51661 gtcaggagta tcagggaaca tcgcaccgaa tgccacacac tggttcatcg tacgagcaaa
    51721 agtagcgccg cctgatacaa acggttcagt catgtctcca gtaatatcgc gatatgtccc
    51781 caatagattt ttcactaact cactgtctag tggcacgtat aatgaatcaa ggaagtcaaa
    51841 ttcttcataa gtcaacccat atttttctgc tgttttagtt aatttagcta ctaaatcatc
    51901 tttcttgaat gtaacaggga ttctcatatc tacaccgatt tttgtttgtt ctggtgtgat
    51961 ttccaaactt gcaaagttca tcgttagctc gccggattgt tcatcttctg ttttgcccac
    52021 gacagcttca ccagttgcat tttcttgaac taattttcct aagaaattga ttgggccaaa
    52081 atcaaatact tctgaaagag caattgccag acgagaaata gcattcactc cttgtgctgc
    52141 atctttcgcg tggatacttt taccaagaac gacgatgcct tcaccttgtt cttcgaaatc
    52201 gaattcgtgt tttttcaatg cttcttttac ttcagaaagc ttttctccag agtaaggagc
    52261 tgcatcagga acaacattca acgctcctcc agcttttaca gaaaactcgt tcgttcctgg
    52321 tcctgtcaag taagcttgca ataagccttt ttctgcataa attaaaggaa attcagcatc
    52381 tggcgcaaat ccttgcgtaa ttccttcttc tttttcgtta tatttttcta aacaacgcca
    52441 taaattttct tcatctgtac caaagataaa acgaatacgt gtcttgaatt ccacgccagc
    52501 atcaatcaaa gctttgacgg catacattgc cgcaatagaa ggacctttgt catcttggga
    52561 tccgcgacca actaaccagc cgtcttttat agttggatcg aatggttttg tttcccaatt
    52621 attttcgtcc cctgctggaa caacgtccat atgacacaag ataccgaata attcatctcc
    52681 agagccaatc tctgaataac cgtaatagcc ttctggatct ttaaatgttc taaatcctag
    52741 cttttcactg atttccaata ctttatccaa agcttcaatc acttttttac cgaagggatg
    52801 tccttgtcct gaatctgctt catctagtac ggaaggaatt cggatcaatt cgctcaatga
    52861 ttctaatgct tgttcatgat gttgttctgt cacaaatttt ttcatgatat tatcctccta
    52921 atatgttaaa aagggtcatg tcaaattcct ccgacacaac ctcttcttct caaataatga
    52981 cgttttaagc ctgctccatc agcaagcaga atgttcttaa caaatgatga aagaaaaaac
    53041 actcactgaa tggagctatt gacttatcca cgtgcttatc gatagccgtt tatcggtttc
    53101 ttcattctta tgatagacct tgtacctgta tttgtctagg tttagaacat tgctgctaca
    53161 cctaggatca cacagatggc tacaaataat atcgcaatta atttaccggt aaatttccac
    53221 cacactgaaa catcgaaacg agcaattgct acagcaccca ttacgatagc agatgttggt
    53281 gtaattaagt ttactaaacc agatgcagat tgataagcag tgattactaa atccttaccg
    53341 acgccggcaa attcacccat aggtcccatg attcccattg ttgcagcagc taagccggaa
    53401 gttgaaggaa tcaagaatga catagggata tagaaaataa atgtaataat gatgaataca
    53461 cttgaagata atgaacttaa tccttgttcg ccccaatgta agatggtgtc tgtgattaat
    53521 ccattgttca taaccacttg gataccacga gcaacagcta ctacgattgc tacaccgatc
    53581 aaatcagaag caccagctaa aaattcagag acaaattcag actctttcat gccataagtg
    53641 attgctacta cgatggacat tactaagaat aacatagtaa tttctgggaa gtaccagtca
    53701 cctaatggaa gcatgctttt acctagaacc gtacctagta ccggaaggtt tgttaaccag
    53761 tttgtcaatg attcaaaaat cgtgaaattc gcattcaagg ttgtccaagg aatcaaactg
    53821 atgatcatga taccaaaagt aataaagaac aatacattaa catgtttttg ttttttcgtg
    53881 attacttctt gacgaccgat tgcttcaata tcgaaatgct tcatatcttc tttacgttgg
    53941 ttgtatacta atgattttgt tggatctttt tcgatctttg atgcataacg atacacatat
    54001 acaatagaaa tagctaacaa aataacgaac atcaagaatc gaagaccaat accttcaccc
    54061 atcgaaatcc caacagcttg tgaagctacg cctgttgcaa aaggatttac cgttgaagct
    54121 agacagccta cttgagaccc tactaatacg attgctacag ctactactgt atcaaagcct
    54181 acagccatca tgactgggat cagtaaagca tagaatggaa tcgtttcttc tgccattccg
    54241 taagtagagc cgcctaatgc aaataacagc atcaatactg gaataagcat tttttctttt
    54301 cctttgaatt tcttcacaac tgaagcaatc ccggcatcta gtgcccctgt tttgttgata
    54361 atacctaaaa atccaccaac aaccaaaatg aagaaagaga ttgggattgc agcaggtgtt
    54421 tctttagtta gctcccccgc tggtacacct agcatcccat tgattggcgc catgaaaata
    54481 tcccacagcc cttgaggatt ttgttgtact cgttcatatg atccagcgac gatgttgcct
    54541 gcatcgtcta cactatagtg cccagctggt ataaaccaag taagtacagc gattactgca
    54601 ataataataa ataagaccgt atacgctgaa ggcatttgaa attttttctt ttttgtttgt
    54661 tccatcttct tcttcctttc ttttccgtta gagacaacaa ttttggctct aatgttattt
    54721 tatcaaaaag agtatcagaa caaaaataat aacacgcatt ttgtaaaact tttgttcaaa
    54781 aagttcgttt ttccctattc cctcctcttt tttcaggttt ttaaagcttt cttctttttc
    54841 attgtagcgt ttcctttttt ttataaacaa acattttcgt cttttaaaag gagaagaatt
    54901 tcacatttgc aaagaattga ttgattttta tgcataaatc aaaaaaatac tcaaaacaaa
    54961 caaataaaac gtttacatta ttgcatgtct tgtatttttt tccaaaaaaa aaagaccaag
    55021 aaatttcttg atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt
    55081 tgttttcgac ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcactcacg
    55141 atgaatcctt tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac
    55201 atgcccagca tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg
    55261 aaataatttg cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa
    55321 aatttgtttc ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta
    55381 aagatcgttg ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct
    55441 gtagtcagca gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg
    55501 aataagaaca tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa
    55561 gccggcatga tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct
    55621 actgcaacca ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct
    55681 cttcagcgaa ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat
    55741 agagttagag ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta
    55801 tactgtcggt atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact
    55861 ataaccaccg gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt
    55921 tacatattca ttatacatat ctattcaaaa aacgcaacat ctaacactta taaaaagtaa
    55981 gtgttcattt tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat
    56041 ccccaggacg taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca
    56101 caggaagacc aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg
    56161 tcaattccac gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg
    56221 gagatgtttt tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg
    56281 tagcgatccc ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca
    56341 ggcgctgagc aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca
    56401 gtcgacctac aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc
    56461 ttcctttgtc atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac
    56521 cgtattcttc ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt
    56581 ttatccctaa ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt
    56641 cttctgcttt tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca
    56701 ttacaattat tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc
    56761 aagtgaaatt tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata
    56821 atctaagaag tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc
    56881 tttttcttca atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc
    56941 tcgaatctgc tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt
    57001 ttcgacggcg gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg
    57061 agccagctct tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc
    57121 tgagtgcgag ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc
    57181 ttcgctgaga tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat
    57241 aaaaagaagg gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat
    57301 aaagcataat tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca
    57361 attctagcaa agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag
    57421 aatcgaaagt acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt
    57481 cttcgatcgt tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac
    57541 gttctaagat tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca
    57601 acggcttttc agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac
    57661 ttcgttcctc tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta
    57721 aaagctgagt gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtga
    57781 cgaattcttg gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca
    57841 ctaagtattc tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat
    57901 attgctccgt gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca
    57961 gatgaagcag cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca
    58021 actcattcac tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat
    58081 ttcagttgca cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa
    58141 gcccatattc tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc
    58201 gttcgttgcc cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa
    58261 gaaaaaaagt tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt
    58321 tttacctgtc agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt
    58381 ccggtaatgt gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt
    58441 ataaacatcc gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc
    58501 cgcgtgacct aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt
    58561 ggcaaagcta tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac
    58621 taactgcttc agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac
    58681 aaatacatgc atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg
    58741 ttccagccat tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc
    58801 aactgtctgt accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc
    58861 actgacgcgc atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa
    58921 cgtttttgtc gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact
    58981 tggtaatttc tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat
    59041 acgctcttgc cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt
    59101 tgctgtcgct tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc
    59161 gacatcctgc catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc
    59221 acgttcatag ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag
    59281 ataatcgttg atttgctctt tcataataat ccccctcatg cctctctatc atatcagata
    59341 ccgcttcatt ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact
    59401 cagacggact ggtttctttt ttctttttca tagcattcct gacagattcc atggaacgtt
    59461 agccgatgat cttttactaa aaaatgataa cgttcttcta ctacttgctc cacttcaagt
    59521 aaaagatcct cttctacttc ctcgatcgtg ccgcagttca agcatagtaa atgatgatgg
    59581 aaatgtttgg cgccttcttt tctcaaatca taacgggcta atccatcatt aaaacttact
    59641 ttatcgacaa ttttcaattc tgtcaagatt tcaagggttc gataaacagt agcaagtcca
    59701 atttccggac tctttttttt cactaaaaag aagatttctt ctgcagataa atgctctttc
    59761 tcattttcca aaagcacaag cagagtagct tctctttgcg gtgtcagctt aaaccccgct
    59821 tcatgtagct gctgttttgt ttttttcatc gccgaaatag cattcatgac catttactca
    59881 accccaacaa tttataataa ttacaaataa aaaaagatat aacactatgt taattaaaat
    59941 tattataagc tagttaagaa aaacatgcaa gggattctca attagatttt ttgactagtt
    60001 ttgtcacacc gttttcttgt gtaatcaatc cttgtttcaa aagattacct aatgcgcgct
    60061 taaatgctcc tttgctgata ccaaaagctt tcatgatttc ttctggatcc gatttatctg
    60121 taaagttgat ttgctgatct gcagaacgtt ctaaaatggt taaaatcatt tgtgcatcat
    60181 ctgatatagc ttcatatgct cttggcttca atgaaagatt cagtacaccg tctggtcgga
    60241 caccaatcac tcgacctttt atctgctcgc ctagacgagg ttcattatat cgttcagaag
    60301 gatggataaa cccaagatag aaatcttccg ttaaaacgta cgtacctgtt aatttcaagc
    60361 ggtatgctgt tcctatcaca tctttgtttt tcatttcttc tgttccatat ttgcttaaag
    60421 atttgaatat tttttcatct gctaaactgc cccaaatacg gtcttttgca tcaactctaa
    60481 gggaaatcat caagcgatcc ccttttttag gccatagctc tgtcattgtc ggcaattcat
    60541 caagagatac gacaaagtct ttatctggaa gaccgatatc gacaaacgca cctaaatctt
    60601 ttcgtgtagc agtaacgatt ccaaaagcgt aatgaccgat acggctttta ggaatttgtg
    60661 tagtgatccg attttcatgt ttttgatttt ggtaaccgaa tccttcaact gcttcaccga
    60721 ttttgtgttc accttctgat ttgcttaagc caaaggtttg tccgtttttt tggacaaagt
    60781 agtttttttc attttcatca ataataatac ctgtaaatat agttgctaat aaatcgttca
    60841 tttttttctc cctctatagt gaaaggtttt cttaatacat ttctttgcta tcataacata
    60901 atgttatcta cttgaatagc tgagccttgg tttggaaagg tgaaaaaagg agtatgacaa
    60961 cagttgtgtc atactcccct ttttcgaata aacggtgtta aacggcaggc tcacagcctc
    61021 tttcatttgc ctaaaattaa ttgctcccat ttcctccgaa ccatccagtt ttacagttga
    61081 tggttacgat gtaaaatggc aaagcacttt ctccaatgcc tttcatcaat gcagattttg
    61141 ttgaagtggc atatagatta taatagcctt ccccattttc gattctggct ttttctaatc
    61201 tgtaacctcc ttcaacgata tatccacgtt ggatacatgt agcaatcact ttgctttgga
    61261 cacctggtgc ccacgtccat gctggattag atgcatcggc aatatcactt gctctggcag
    61321 atttgattgg ctgattcgct gagtttgaag aggaaggggt cgaggatgcc gcttcttgct
    61381 gtgctgcttg tgcgaccgca gcttgttgag cggcttgagc tgcagctgct gcttgagcgg
    61441 cttgttttgc ttgttcttca gctgcttttt tcgcttcttc tgcttttttc ttttcctctt
    61501 cttctttttt ctctttatca gtgagcatct tatctacttg tttcaattgt tcattcaact
    61561 gagcagatag atctgtattt ttcacttttt ctactgcttc ttttgccgtc ttgtattggt
    61621 cgcgtgttgc tgaatccact acttttccgt ctttaaagac tatagctgtt ttttctttag
    61681 ctgtttgcaa ttcagcatat tgatttttag cttctgtttg tgcttgttga atcaattttc
    61741 caaaatctgt gttatccgtt tcgattgctt ggatctcttt atctgctttc aacttgatgt
    61801 catctgctag atgatcatcg acgatggcag gtgctatgaa ccactcattc actttttgga
    61861 tatctcggat cttagattgg atatcttggt aagttgtttc taactctgaa tatccttttt
    61921 cattcttaag actggataaa tcatttttta gttttgataa atcttgatga atcatatctg
    61981 cacgaataaa ttgatggtga tcatctgtat aaaaggcagc aagtttctca tgaagttctt
    62041 ccatttgttg ctgtcttttt gcttctgctg ctacttcggc ttctactttt tgatgatggt
    62101 cataataagc ccagcttcct ccaccgagta gaagtactgc tgctaaaacg agataaggta
    62161 ctttttttga ttttttcttt tgcgctttat ctggatctgt tttttctaag tttgattctt
    62221 gatgtgtttt ttcagtatct ggcacgattg ttattgattt cgcttctgct tcaaacagct
    62281 taggctggtt ctcttctttt tctatttttg cttcaagatc agtaccctct ttgcttacag
    62341 attgattttc agcatcggat tcactattta tttcatcggg ctctgttgac tttttgtctg
    62401 tgttttcgag tatttctttt ttctctgctt ccggcagttt attcgttttt tccttttcta
    62461 attgtttttc agtaaatact tcagtttttt ctgtgatatc cgtttttacg tcttttattt
    62521 ttactgtttc tttacctgtg gcgttttctc tatcagagtc tttttcagtt tcttgttcct
    62581 tttctggctc tactttgttt tcctgtgcaa ttttttcact gatcattttg agcagctctt
    62641 tcgcgtgctc ttcttttgta tcttcacgat gtgctttgat ataagcggat agtatcgaat
    62701 tttcttcctc attatcgacg atttctttca gttcagctac tcttcttttt tgttttgatt
    62761 caagcgtttc ttcttgattc tttcctgatt cttccgaagt ctcatgaaca gttgctttct
    62821 tctgttcatt tttctctata ttagttactt gatcgtgagc aggtggttct tcgtcttctg
    62881 gttctgcttt ttcgaataat tccattacag attctagagg taaatctttc aattccgacc
    62941 attcgattgg atcgtttaat tctgtatcag gaaagactgc atcttcagtt gatttatcct
    63001 gcatagctgc atctgtattt gtttcttcaa ttgattcttt tatttgctga tcttctgcag
    63061 ttatttgatg accgcagtct ggacagatag tattctgctc catttttttc gatccgcaat
    63121 ttggacattt tttcatcact tagcgatcgg ctcctttact aggtttacgc taccatttaa
    63181 tctatcatat tctatacgtc acgccaatag cttattttta ggatttactt aaaaataatg
    63241 aatgaatttc cttcctatag aaaaaaaagc ttgagactgc gtagtctcaa gcttttt
//
LOCUS       DACDIH010000008        62273 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDIH010000008 DACDIH010000000
VERSION     DACDIH010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.008"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..120)
                     /gene="rpmH"
                     /locus_tag="IV281_000513"
     CDS             complement(<1..120)
                     /gene="rpmH"
                     /locus_tag="IV281_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP8779554.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRK"
     gene            643..1977
                     /gene="dnaA"
                     /locus_tag="IV281_000514"
     CDS             643..1977
                     /gene="dnaA"
                     /locus_tag="IV281_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP8779555.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            2175..3305
                     /gene="dnaN"
                     /locus_tag="IV281_000515"
     CDS             2175..3305
                     /gene="dnaN"
                     /locus_tag="IV281_000515"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP8779556.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            3534..3776
                     /gene="yaaA"
                     /locus_tag="IV281_000516"
     CDS             3534..3776
                     /gene="yaaA"
                     /locus_tag="IV281_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP8779557.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            3763..4887
                     /gene="recF"
                     /locus_tag="IV281_000517"
     CDS             3763..4887
                     /gene="recF"
                     /locus_tag="IV281_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP8779558.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            4884..6830
                     /gene="gyrB"
                     /locus_tag="IV281_000518"
     CDS             4884..6830
                     /gene="gyrB"
                     /locus_tag="IV281_000518"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP8779559.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            6850..9321
                     /gene="gyrA"
                     /locus_tag="IV281_000519"
     CDS             6850..9321
                     /gene="gyrA"
                     /locus_tag="IV281_000519"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP8779560.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            9557..9856
                     /locus_tag="IV281_000520"
     CDS             9557..9856
                     /locus_tag="IV281_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP8779561.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            9906..10418
                     /gene="ssb"
                     /locus_tag="IV281_000521"
     CDS             9906..10418
                     /gene="ssb"
                     /locus_tag="IV281_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8779562.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            10444..10680
                     /gene="rpsR"
                     /locus_tag="IV281_000522"
     CDS             10444..10680
                     /gene="rpsR"
                     /locus_tag="IV281_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP8779563.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            10823..12796
                     /locus_tag="IV281_000523"
     CDS             10823..12796
                     /locus_tag="IV281_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP8779564.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            12802..13254
                     /gene="rplI"
                     /locus_tag="IV281_000524"
     CDS             12802..13254
                     /gene="rplI"
                     /locus_tag="IV281_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP8779565.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            13528..14895
                     /gene="dnaB"
                     /locus_tag="IV281_000525"
     CDS             13528..14895
                     /gene="dnaB"
                     /locus_tag="IV281_000525"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP8779566.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            15675..16967
                     /locus_tag="IV281_000526"
     CDS             15675..16967
                     /locus_tag="IV281_000526"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP8779567.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            17754..18617
                     /locus_tag="IV281_000527"
     CDS             17754..18617
                     /locus_tag="IV281_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8779568.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(18746..20368)
                     /locus_tag="IV281_000528"
     CDS             complement(18746..20368)
                     /locus_tag="IV281_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8779569.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            20617..21138
                     /locus_tag="IV281_000529"
     CDS             20617..21138
                     /locus_tag="IV281_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779570.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGTIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(21324..23054)
                     /gene="spxB"
                     /locus_tag="IV281_000530"
     CDS             complement(21324..23054)
                     /gene="spxB"
                     /locus_tag="IV281_000530"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP8779571.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            23219..24037
                     /locus_tag="IV281_000531"
     CDS             23219..24037
                     /locus_tag="IV281_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP8779572.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(24139..24336)
                     /locus_tag="IV281_000532"
     CDS             complement(24139..24336)
                     /locus_tag="IV281_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779573.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            24505..25083
                     /locus_tag="IV281_000533"
     CDS             24505..25083
                     /locus_tag="IV281_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8779574.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(26128..26631)
                     /locus_tag="IV281_000534"
     CDS             complement(26128..26631)
                     /locus_tag="IV281_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8779575.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            26990..28015
                     /locus_tag="IV281_000535"
     CDS             26990..28015
                     /locus_tag="IV281_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8779576.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            28338..29216
                     /locus_tag="IV281_000536"
     CDS             28338..29216
                     /locus_tag="IV281_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779577.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            29692..29808
                     /locus_tag="IV281_000537"
     CDS             29692..29808
                     /locus_tag="IV281_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8779578.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            30736..31074
                     /locus_tag="IV281_000538"
     CDS             30736..31074
                     /locus_tag="IV281_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779579.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            31168..31398
                     /locus_tag="IV281_000539"
     CDS             31168..31398
                     /locus_tag="IV281_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779580.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            31798..32169
                     /locus_tag="IV281_000540"
     CDS             31798..32169
                     /locus_tag="IV281_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP8779581.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(32443..33135)
                     /locus_tag="IV281_000541"
     CDS             complement(32443..33135)
                     /locus_tag="IV281_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8779582.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(33138..33923)
                     /locus_tag="IV281_000542"
     CDS             complement(33138..33923)
                     /locus_tag="IV281_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8779583.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            34160..35560
                     /gene="lacG"
                     /locus_tag="IV281_000543"
     CDS             34160..35560
                     /gene="lacG"
                     /locus_tag="IV281_000543"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP8779584.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            35585..35902
                     /locus_tag="IV281_000544"
     CDS             35585..35902
                     /locus_tag="IV281_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8779585.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            35915..37588
                     /locus_tag="IV281_000545"
     CDS             35915..37588
                     /locus_tag="IV281_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8779586.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            37602..39233
                     /locus_tag="IV281_000546"
     CDS             37602..39233
                     /locus_tag="IV281_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP8779587.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(39724..41289)
                     /gene="guaA"
                     /locus_tag="IV281_000547"
     CDS             complement(39724..41289)
                     /gene="guaA"
                     /locus_tag="IV281_000547"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP8779588.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(41348..42271)
                     /locus_tag="IV281_000548"
     CDS             complement(41348..42271)
                     /locus_tag="IV281_000548"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP8779589.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            42467..43072
                     /locus_tag="IV281_000549"
     CDS             42467..43072
                     /locus_tag="IV281_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8779590.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            43380..44396
                     /locus_tag="IV281_000550"
     CDS             43380..44396
                     /locus_tag="IV281_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8779591.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            44537..45838
                     /locus_tag="IV281_000551"
     CDS             44537..45838
                     /locus_tag="IV281_000551"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP8779592.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            45884..46546
                     /gene="deoC"
                     /locus_tag="IV281_000552"
     CDS             45884..46546
                     /gene="deoC"
                     /locus_tag="IV281_000552"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP8779593.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            46567..46965
                     /locus_tag="IV281_000553"
     CDS             46567..46965
                     /locus_tag="IV281_000553"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP8779594.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            47031..48113
                     /locus_tag="IV281_000554"
     CDS             47031..48113
                     /locus_tag="IV281_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8779595.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            48325..49887
                     /locus_tag="IV281_000555"
     CDS             48325..49887
                     /locus_tag="IV281_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8779596.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            49880..51013
                     /locus_tag="IV281_000556"
     CDS             49880..51013
                     /locus_tag="IV281_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8779597.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            51013..51969
                     /locus_tag="IV281_000557"
     CDS             51013..51969
                     /locus_tag="IV281_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8779598.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            52068..53234
                     /gene="deoB"
                     /locus_tag="IV281_000558"
     CDS             52068..53234
                     /gene="deoB"
                     /locus_tag="IV281_000558"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP8779599.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            53258..54076
                     /locus_tag="IV281_000559"
     CDS             53258..54076
                     /locus_tag="IV281_000559"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8779600.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            54093..54800
                     /gene="deoD"
                     /locus_tag="IV281_000560"
     CDS             54093..54800
                     /gene="deoD"
                     /locus_tag="IV281_000560"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8779601.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(55098..55784)
                     /gene="gpmA"
                     /locus_tag="IV281_000561"
     CDS             complement(55098..55784)
                     /gene="gpmA"
                     /locus_tag="IV281_000561"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8779602.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(55956..56537)
                     /locus_tag="IV281_000562"
     CDS             complement(55956..56537)
                     /locus_tag="IV281_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8779603.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(56670..57353)
                     /gene="rpiA"
                     /locus_tag="IV281_000563"
     CDS             complement(56670..57353)
                     /gene="rpiA"
                     /locus_tag="IV281_000563"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP8779604.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            57672..58085
                     /gene="rpsL"
                     /locus_tag="IV281_000564"
     CDS             57672..58085
                     /gene="rpsL"
                     /locus_tag="IV281_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP8779605.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVRD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            58169..58639
                     /gene="rpsG"
                     /locus_tag="IV281_000565"
     CDS             58169..58639
                     /gene="rpsG"
                     /locus_tag="IV281_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP8779606.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGISANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            58733..60820
                     /gene="fusA"
                     /locus_tag="IV281_000566"
     CDS             58733..60820
                     /gene="fusA"
                     /locus_tag="IV281_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP8779607.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            60992..62179
                     /gene="tuf"
                     /locus_tag="IV281_000567"
     CDS             60992..62179
                     /gene="tuf"
                     /locus_tag="IV281_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8779608.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
BASE COUNT        19671 a        11157 c        13118 g        18327 t
ORIGIN      
        1 ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag tactcatacg
       61 tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg ttcttttcat
      121 atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact ttgatagtat
      181 aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa aacttatcca
      241 caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag ggattatcca
      301 cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt aatccacaat
      361 caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt ttttatccac
      421 agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt agtggtgtgg
      481 aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg tgtggataat
      541 tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa aatgctaaat
      601 tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc cctcgatgct
      661 ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag ctataataca
      721 tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt tgaggttcct
      781 agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt tgaagtcggt
      841 tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga agcagagcaa
      901 gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct aaaaaaagcc
      961 atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa ccagatggct
     1021 catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc gctcttcttt
     1081 tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca tcaaatgctg
     1141 caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc taatgatttc
     1201 atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg aaatgttgat
     1261 ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca agaagagttt
     1321 ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac cagtgatcgg
     1381 ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc atgggggctg
     1441 tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg taaaaaagcg
     1501 gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg acaaattgat
     1561 tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc tgcgatgaat
     1621 agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa atcaggaaaa
     1681 ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta ttatcacatc
     1741 caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc tagacaaatc
     1801 gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg agctgaattc
     1861 ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca gctgatggat
     1921 tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact aaattaattt
     1981 gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc aaaagttata
     2041 cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt tttccacaga
     2101 atcaacatgc cctattactt ttattattct ttttaactaa taataaataa acaaacattc
     2161 aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag gaattgcaaa
     2221 ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt gtaaaaatca
     2281 cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca attgaaactt
     2341 ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt tccattgttt
     2401 tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca tttactttag
     2461 aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt atcgtaaatg
     2521 gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac cagatgaaat
     2581 tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc tcacaacatg
     2641 agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct ttattagctg
     2701 tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa gcggctgatc
     2761 attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca ttaacaaatg
     2821 aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa acagaaacga
     2881 tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt ttgattcctt
     2941 caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg atcgaacggg
     3001 cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt cgtccagatg
     3061 cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc ttgagttata
     3121 cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg aaagcagcgt
     3181 tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt cgtccattta
     3241 cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct gtacgtacaa
     3301 attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga aaaaataaaa
     3361 ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt gtttaaatca
     3421 ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg ttttattgcg
     3481 aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg gcattgaaac
     3541 aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa gaagtcacag
     3601 ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg ttagtcgatg
     3661 gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt gaagttccag
     3721 aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct gaataagctg
     3781 tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa gaacctcgtc
     3841 atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat ctacgtatta
     3901 gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg gactgacgat
     3961 caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt agaaatctta
     4021 ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa gttaagtact
     4081 tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt agtaaaaggc
     4141 agcccgcaga tcaggcggaa gtttttagat atggaattag gccagatcaa tccaatctat
     4201 ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta cttgaagcaa
     4261 ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga acaactagca
     4321 gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt ggagttttgg
     4381 gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat cgagtatctc
     4441 accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca atttctcgca
     4501 cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt tggtcctcat
     4561 cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg atcgcaagga
     4621 cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat taaagaagag
     4681 acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga tgataatcgt
     4741 caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac aacaacgaca
     4801 cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt tcaacaaggg
     4861 aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa cgcgctaaag
     4921 agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt aaacgacctg
     4981 gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg gaaattgtcg
     5041 ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg attattgaaa
     5101 aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat atccaggcaa
     5161 aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga ggaaaattcg
     5221 gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct gttgtaaacg
     5281 cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac tatcaagaat
     5341 atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat cgccatggaa
     5401 caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag tttgattttt
     5461 caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg cgtatatcga
     5521 ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac ggcgggatca
     5581 aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa cctgtatatt
     5641 tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca gacggctacc
     5701 attccaatat catgagtttt gccaacaata tccatacgta tgaaggtgga acacatgaat
     5761 caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa caaaagctga
     5821 tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg acagctgtta
     5881 tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa ttgggcaatt
     5941 ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa ttcttgctgg
     6001 aaaacccaag tgtgggccgg caaattgttg aaaaaggatt actagcttcc agagctcgtc
     6061 tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag attagtaatc
     6121 tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa ttgttcatcg
     6181 tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag tttcaagcca
     6241 tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac aagatcttgg
     6301 caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga gaagattttg
     6361 atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat gtcgacggcg
     6421 cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct attgttgaag
     6481 ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg aagaatatta
     6541 cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt gcttcacttc
     6601 ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg gatgatcatc
     6661 aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc agtgtggacg
     6721 atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta gaaccccgtc
     6781 gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa tcatgaagga
     6841 ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct aaccagcgaa
     6901 atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc acttcctgat
     6961 gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa cgaattaggt
     7021 gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt catgggtaaa
     7081 taccatcctc atggagacat tgcgatctat gagtcaatgg tacggatggc acagccattc
     7141 agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga cggtgacggt
     7201 gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga aatgctgcgt
     7261 gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga aaaagaacca
     7321 gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg gatcgccgta
     7381 ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc tatcgatttg
     7441 ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc tggaccagat
     7501 tttccaacag gtggtttggt tatggggaaa tcaggaatcc gaagagcgta tgaaacaggt
     7561 aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa tggtaaagaa
     7621 cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat cgaacggatt
     7681 tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga cgaatcttcc
     7741 cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc cgttatcttg
     7801 aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat gttggcgatc
     7861 gaaaaaggtg tgccgaaagt tttgagcttg aaacaaatcc ttgaaaacta tatcgaacat
     7921 caacgcgaag tcatcacacg acggacggtc tttgaaaaag aaaaagcaga agcaagagca
     7981 cacatcttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat tgcgattatc
     8041 cgcgggtcta aatcagatga cgaggcaaaa gcaacaatga tcgaacggtt cgatttatcc
     8101 gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg tttggaacgg
     8161 gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt aactgacatt
     8221 ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga cttgaatcaa
     8281 cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag tctggaagat
     8341 gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg ttacatcaaa
     8401 cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt ccaaggaatg
     8461 ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca tgatacattg
     8521 ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat tccagaatac
     8581 gggcgtacag ccaaaggaat cccaatcatc aatttactag gtatcgattc gagtgaaagc
     8641 atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct cttctttacg
     8701 acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat ccgaagtaat
     8761 ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct actgacagat
     8821 ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt caaagaagaa
     8881 gcagtccgcg atatgggacg tacagcttcc ggggtcagag ggatacgcct gcgtgaggaa
     8941 gattatgtcg ttggagcagc cttaatgaag gaaaatcaag aagtattagt gatcacagaa
     9001 aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg cggaggtaaa
     9061 gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt gaccactgtt
     9121 tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg tttcaacgta
     9181 tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa gatggaagct
     9241 gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca aacagttgaa
     9301 gaacaaggat cagaagaata accatcaaga ctaagtgtgt aagctagcaa gaataaccga
     9361 ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc ttaaaatatt
     9421 cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatta gagcttgtga
     9481 gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc gaaagtccac
     9541 agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta ttcgtccaaa
     9601 cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct tgaaagataa
     9661 cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt atgaaatgaa
     9721 cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat cagcaaatgc
     9781 tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc acatgattgt
     9841 taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga gaggagatga
     9901 ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca gatttgcgtt
     9961 acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt aattttacaa
    10021 accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc aaatcagcag
    10081 aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga cgtatccaaa
    10141 ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta gtagctgaca
    10201 acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc ggcggattcc
    10261 aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat caaacatctc
    10321 aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc ggctcaagtt
    10381 caacgatcga catttcagac gatgatttac cattctaaac gaatttgata ggagggaaat
    10441 agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta cattgctgct
    10501 aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt tatttcagaa
    10561 cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca acgtaaaatt
    10621 actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag cgaagactaa
    10681 taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc tttttttctg
    10741 taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta aaatcaaatt
    10801 agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc cgaaacctct
    10861 cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac catttcgttt
    10921 tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt ggttgaaaat
    10981 gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga ctgtagctga
    11041 aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt atcagccatc
    11101 aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc aaagtggatt
    11161 aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga aaaaccaagt
    11221 ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg ttgtttattt
    11281 taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc agccggtgat
    11341 tggtatttta tctattgata actatgcaga tgccattgat aaattagatg acaaagagat
    11401 ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg aataccatgt
    11461 ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga tcgatctgaa
    11521 aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag ctgaaaaaca
    11581 gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc tcgatgaaat
    11641 cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg accaagttgt
    11701 cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat ctgctaagaa
    11761 tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc ggatttttga
    11821 tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg ctttaggctc
    11881 tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca tcgttatcaa
    11941 tgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata agcatcctga
    12001 attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag aagaggacct
    12061 cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc tatatgagca
    12121 atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc caagtaaacc
    12181 tctgctgact tatattgaat cttcagcttc ttcagcttca gagctagttt ctgagctgat
    12241 ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat tattgcttgc
    12301 agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa cttttgacgt
    12361 tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt tattgagctc
    12421 ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact atgtgacttc
    12481 tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta tagcagcaaa
    12541 aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca ttacaaagcg
    12601 gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg tccaaatcat
    12661 catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac tggaaaatac
    12721 gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata tgaatcaaat
    12781 atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa aggaaaaggt
    12841 aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct aatcaaaaat
    12901 aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca aaagaaagca
    12961 caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga attcttggaa
    13021 aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg tttgtttggt
    13081 tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat caaattagat
    13141 aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa cgtacctgtt
    13201 aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga ataacaaatc
    13261 ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa ccgaaattta
    13321 tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca gattgatttc
    13381 ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata aaagagaact
    13441 atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct tgtaaaatga
    13501 agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg aatcccaccg
    13561 caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc agaaacgatc
    13621 attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca tcaaattatt
    13681 tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt tactgtcaaa
    13741 gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta tctctcagac
    13801 ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat cgttgaagaa
    13861 aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa aggattcgag
    13921 caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt ggaggtttcg
    13981 gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc cacgattgaa
    14041 aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac tggctatcaa
    14101 ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct tgctgcacgt
    14161 ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg aacaaaaaca
    14221 gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt aaatcggatg
    14281 ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct ttcggaagaa
    14341 gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat ttttatcgat
    14401 gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact agcgcaagaa
    14461 aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg taacggtaaa
    14521 gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact ggcgaaagag
    14581 ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca gcgacaagac
    14641 aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga tgcggacatc
    14701 gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga cgatgatgga
    14761 aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg aagcggtgca
    14821 cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc gctttcgcca
    14881 agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc tctcgtaaga
    14941 agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga tctccaaagg
    15001 gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt caatcagctg
    15061 acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac gtttgtttct
    15121 ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg tgttctcctg
    15181 ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt tttgaaattg
    15241 ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac ggtgttcaaa
    15301 agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct atttttgttt
    15361 ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc acggtgttcc
    15421 aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa gcaattttcg
    15481 tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt tttaataacc
    15541 attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat aaaaatcatt
    15601 cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta aataatatag
    15661 aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc gatgaaggga
    15721 aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg taccaaggtg
    15781 gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg catttgattc
    15841 cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt gtagtcaatc
    15901 ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa acaagtaatt
    15961 tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg gaccaattac
    16021 aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc ggacctgcct
    16081 atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat aaagaaattt
    16141 ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta aaaatgtttg
    16201 attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac ggacaacaaa
    16261 tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat gcaggcaaac
    16321 gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga acttatcctt
    16381 ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt gtagggcctt
    16441 caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc ggtgatggac
    16501 catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta ggacgagaat
    16561 acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta gttatgcgcc
    16621 attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat gtattaagtg
    16681 gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta atctaccact
    16741 atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa ttgccaggct
    16801 ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac gcacgtaact
    16861 atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct gtcggacctg
    16921 atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga taaaaacgac
    16981 gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac gataataata
    17041 acttgaagag gtagtgaaag agacgtttag ccttattgcc gataggctgt ctcttgaaca
    17101 ctgtttattc agaataaggg actgtgacaa aaaccgtgtc acagtccctt tttccgaaca
    17161 aacggtgttc aaaagcttgc atctacggca ctaagcccaa tcagatgaaa aatatggagt
    17221 gctattttta tctgattgtt cttactgctt gatgcagagc agctttttca caacctctcc
    17281 actaaacggt gttctcctgc caactcctcg gtcacaagtc acaatattca acaaacatga
    17341 gaagctgttt tttgaaattg ttccttgtga ctcggagcta aacggcaggc tcacaacctc
    17401 ttggtaaacg gtgttcaatc agctgaccct cggtattagc tcaaaaaacg gcaaaatatg
    17461 aggaactatt ttttccgttt ttttctctaa tactcaggtc aaaacgctga tttcacaacc
    17521 tctgattcac ggtgttccag aagtagcttc tacggaaata agccgaaatt tcacaaaaat
    17581 ttgagaagca attttcgtaa attccttctt atttcttgaa gctgaccact tctgtcacaa
    17641 cctcttattt tgattagaac ttgcgaaaat aaaccggtta aagtgtgtct agcgcaagag
    17701 atttgctata atagacgata agggtaaaat gtaacgagaa ggggttgaaa aatgtgtatc
    17761 aaattgtaac agattcttgt tgtgatctgc cataccatgt attagcaaaa gaaaaagttg
    17821 attttttatc aatgaatctc caattgaatg atcaagagtt agtagatgac ctcggaaaga
    17881 cattcaatta tgaccgctac atgaatcagc tgaaaaatgg tgcgatgcct acgacatcac
    17941 aaatcaatgt tggccgatat ttagaatttt tccgttctta tgcggaaaaa cagattcctg
    18001 ttttatatgt ggctttttcc agtggattaa gcggctctta cagtagtgcg cttcaagcgg
    18061 ttgaattgct gaaggaagaa tatgaagacc ctgagatcta tgtttttgat acaaaagctg
    18121 caagtattgg ggaagggctt ttagtattgg aagctgtaag attgaaaaaa gaaggtaaaa
    18181 gcatcgaaga aaccctagag tggctggaaa gtaattattt gaaagtccat tcatgggtaa
    18241 cagtagacga tctgaaatat ctagaacgag gcggtaggat ttccaaagca agtgctactc
    18301 ttggcagtct attgagtgta aagcctatta ttacggtgga taaagaagga aggctgacga
    18361 atgttgacaa agttcgagga agaaacaagt cgatccagac aatcgtcaat gagacggtaa
    18421 aaacaatcgt tgcaccactt gatcaaatcg tttatgtggc ttacgctggg gatctagaga
    18481 gcgcggagaa ggcaaaacgg cttttggaag agcagataaa gatgaaagat gtgaagcttt
    18541 atcccttagg accaacgatc gccagtcaca caggctatgg ctgtatcgcg attttttcga
    18601 tgggaatcag tcgataaaag cagtaactca ttctgtaaaa aagcccagaa attgattgta
    18661 acaaaaacag cgactcttaa aagatagatg agcatcctat ctcttagagg tcgctgtttt
    18721 tttatttttt aagctaaata ggccattatg cccataactc gttcaattgt ttttgaatat
    18781 ctgcattttc caaaaattca tcatatgtcg tatcagctcg atcgatcaca cctttatccg
    18841 aaacggcaat gatgcggttt gccaatgttt ggataaattg atggtcatgt gacgcaaata
    18901 agattgagcc agtaaatgcc atcaagccgt cattcaatgc tgtgattgat tctaagtcta
    18961 aatggttcgt tggatcatct aatacaagta cgttagcttt cgaaagcatc atttttgaaa
    19021 gcatgcaacg gactttctct ccaccagata aaacattcac tggtttcaat acttcttcac
    19081 cagaaaacaa cattcttcct aagaagcttc ttaagaaggt gttatcatct tcttctttac
    19141 ttgcatattg acgcaaccag tctaaaatcg ttagatcagt atcgaattct ttcgttgtat
    19201 cctttggtaa ataagaatta cttgttgtca caccccaacg tactgttcca gtatctggcg
    19261 taatttcacc catgataact ttgaatagcg ttgttgttgt tatatcattt tctgcgacga
    19321 aagcgacctt gtcttctttg ctcaaattaa aagatatatc atctaaaatt ttctttccat
    19381 caatcgttac acttacattt tctacctgca ataagtcgtt tccaatctca cgttcaggtt
    19441 tgaatccaac gaatggataa cgtctagaag aaggttggat atcgtctaaa gtaatttttt
    19501 caagcatttt tttacgtgaa gttgcttgtt ttgattttga tgcattcgca ctaaagcggg
    19561 caataaattc ttggagttct ttgatttgtt cttctttttt ggcatttgcg tttgcttgaa
    19621 gtttagccgc tagttgacta gattccaacc aaaaatcata atttcctaca taaagtttga
    19681 ttttaccaaa atctagatcc gccatatgtg tacatacttt gttcaaaaag tgacggtcat
    19741 gggatacaac gatcacagta ttttcaaaat tgatcaaaaa ttcttccagc caattgatcg
    19801 attggatatc cagaccgttt gttggttcgt ctagcaaaag aacatcaggt ttaccaaata
    19861 gtgtttgtgc taataacact tttacctttt gtccaccagt taattcactc atttgctgat
    19921 ggtgcaattc ttcagggatg ttcagacctt gcagtaaaac agctgcttct ggttcagctt
    19981 cccatccatt aagctcagca aattctcctt ctaattcagc tgctcgaata ccatcttcgt
    20041 cagtgaaatc ctctttcatg taaatcgcat ctttttcttt cagcacttca tacagacgct
    20101 tgtgtcccat gatgactgtt tcaagtacag tatagtcttc atagtcgtaa tggttctgtt
    20161 tcaatacggc catccgttca tctggtccca tagtaacgga acctgtggaa ggctgcagat
    20221 cccctgataa cactttaagg aacgttgatt tacctgcacc atttgcacca atcaagccat
    20281 agcagtttcc gggggtaaat ttaatattca catcatcaaa tagtttgcgg tctgaaaagt
    20341 gcaaactcac atcatttaca gtaatcaatt tttttcctca cttttatcat tattagtttt
    20401 gacaagtctt atagaattat ataggggaaa ggtaaaagat tcaaggcaat cgaacattct
    20461 ttgtgtttca cgtgaaacag aatgaaaaaa ataaaaataa aagcggagcg aaaatagcta
    20521 ctataggatt tattagtttg caacagcata atttttgacg ttatttataa gagggagtac
    20581 tataaaactg taaaagaaat gaaagaagga ttgaaaatgg atgaaaaaag aaccatcgac
    20641 tggggctcat tgattttggg aattttattt gtacttgtct cattgttatc atttcaagac
    20701 cctgtcggaa atttagttgc aattgtagta gtctttgcga tttttgcctt tattaaaggg
    20761 atatttgaac tgtttgtacg gaacagatta aaagagctaa caggatataa aggaaagatg
    20821 cccttaataa tcgggaccat cgatattcta gttggcgtct ttttcttatt caatattggt
    20881 gcaggagtcg cagcattgcc ttttgttttt gcagtatggt ttattgctga ttcagtttta
    20941 gcgttattta ctgctgactt ggcaagagga gtaagtgaag gatattattg gtttacgatc
    21001 atcgtcaata ttttaggaat tttattagga attttcttat tgtttaatcc aatttcttct
    21061 gcgttgacat taagcttttt agttggattc tattttatgt tatttggtat cacacatatc
    21121 gtctatgcat ttagataagt aaaatcaaac aaataaaaaa gccggcttcc gattacagtt
    21181 ttactgttgt aattggaagc cggctttttt atcatggaca aatatgcacg aggtgatttt
    21241 taggtgtcga gacaaattta tgcaaaaatg tgagaagcta tatacacaaa ttttatctta
    21301 atactcgaag catagcgcag ttgtcacaac ctcttttcat ataattcatt caatactggc
    21361 aaatcatgga cttcatattt ttctttgaat gcttggattt cttcttcaga atattttctt
    21421 ggatctaaat gcagatcttc tactggcaat gggcgcttgt tattgatctt cacatcgatt
    21481 actacaggac gatctgattt tgcagctgca tcaaaggcaa atcgaagttg cgagtattct
    21541 gtgactgtaa agctatttgc cccgagcgct tctccaactt taccgaaatc tgcatcttct
    21601 aaaaatacac caaatttctt ttgttccgta tcttcttgtt ctgcttcgat gaaaccaaaa
    21661 gaatcattcg aaaagacaac attaataatt ggaagctgat atttgacttg tgtaatgata
    21721 tcttgcatga ccatagcaaa cccgccatca ccacttaagg taaatacttg tttttctggg
    21781 tagcttagtt gtgcggcaat tcctcctggc acaccattac ccatagttgc gaaccatcca
    21841 gatgtcgtat gtttttgttt accgttcata ttcaataaac gaatagaatg gatagtcgtg
    21901 ttaccgacat ctgtgacgaa aatcgcatcg tcttcagcaa tacgattgat ttctttatag
    21961 acagcctctg gacgaatcgg ttcttcctct cgatcttcaa aagatttcaa ccaatttacc
    22021 catttttctt tattttcttg atttgctagg taccagctat cagcagggcg agcatcccct
    22081 aactcgacaa gtctttgcaa agctgtgttt gcatcaccga ggatcgacaa agatgtttca
    22141 tggcgtcgtc caaattttga cgcatcgata tcgatttgga taaattcagc agttggatta
    22201 aaaaatgcac gtccaaatgg gaagtcgcta ccgacaaata agatcagatc ggcttctgcc
    22261 aatgcttcat ttccgggttt agtagcgaca cgtccagcaa aaccaagaaa gttttcataa
    22321 tcatcaggaa taattccttt tgctagaacg gaagcgacaa caggaactga aaaatgttct
    22381 gagaattctt tgatttgcgg aaatccgttt cgcgttcctt gaccaacata aagaactggt
    22441 ttttttgctt tttcaagata aggcaaagca gctagcaaat ctttttcttc tggtaatatc
    22501 acacttgttt tgtgagtgtg agcggtggag aaaggttgtt cgtcaatttc ttcaaaacca
    22561 agatcaacag ggatagtcac gatagccacg cctttatgct cataggcggc tttaatggct
    22621 tcatctacta catgcggaag actttgtggt gtcatgactg tacgattgta tacgctaaca
    22681 tctgcgaaaa taggattttc atttagttcc tggaaagagt tatagttcat ggatgtagaa
    22741 gcaacttgtc ctaagaacgc tactagaggc acatggtcca tctgtgcatc atacaaacca
    22801 ttgatcaaat gtgtcgcacc aggtcctgca gagccgaaga cagcgccgac tttccccgtt
    22861 aattttgcat ctgcagcggc tgcgagtgcg ccgacttctt catgacgaac ttgaatgtag
    22921 ttgatttcgg ttttttcttg atatagcgca tccataattg aattgaaaga tcctccggga
    22981 atgccgtaaa tatggtcgat accccagctt tcaagaactt ttaccattgc gattcctgca
    23041 ttgatttttg acatggtgtt tcactccttt agcaatgatg atacataaat tttttgtatc
    23101 tagagtatag aactttttat cgaaaatccc aactaattcg tcttttcaca aaaatgtagt
    23161 catgtttttg ggaatatgct agagtagtac atagatagat tcaagagagg ggaatggcat
    23221 ggttgaatgg ctattgagac ttgaaccttg gcagcaagca cttgctggga cagcttttac
    23281 ttattttatg acagctcttg gtgccgggtt ggtttttttc tttaaagaaa tcaaaaaaga
    23341 agtgctaaac ttgatgttag gatttgcttc tggtgtcatg attgctgcaa gtttttggtc
    23401 tttgttagat ccggctatta caaaagcaga agaaaatgga gatattgctt ggctagttgt
    23461 aagtattggg tttggtttag gcggtgtgtt tttatatatg gcagataaaa cactgcccca
    23521 tatgcatttt ggaccacaac atgaagcaga aggattgcct acccatttaa aacggacgat
    23581 cttgcttgtt ttttctatta cattgcataa cattcctgaa ggtttagccg tgggggtagc
    23641 attcggtgca gcagcaactg cggataatcc gacagccgct atattagccg ctgtttcggt
    23701 ggcgctaggt attggtatcc aaaacttccc agaaggagca gctgtatcaa tcccgttgcg
    23761 acaagaaggt ctgagtcgta aaaaggcctt tgtttacgga caagcttctg gaatcgttga
    23821 accaattgct ggagtgattg gagcgctact agtaacgaaa gtggagctgc tgcttcctta
    23881 tgcacttgct tttgccgcag gagcaatgat ttatgtcgtg gtggaagaat tgatccctga
    23941 agcacagcag acattgtcca gcaaacgtca ttttgcagtt tttggcgtca tgagtggatt
    24001 tatcattatg atgattttgg atgtggcatt aggataaata aaaaaatata tatgatatat
    24061 caaatagggc cgcgaactta tatcgcgacc ctattttttt agtaagttat atcataatga
    24121 agtgaatggt atcatacatt aataatagtt ttttctatgt gtatttcctt tgatccagcg
    24181 aacaagccag atgataagtg cgattgggat tagaactttg accgcaaacc ataaaagccc
    24241 gcttaggaca ctgacaaaaa gactgatgac gaagctagct gcaattagca ctaaaagaac
    24301 catgaagata tttaagaaca tatttttttg acgcatacaa aaccctcctc ttgttcattc
    24361 tttactatag aaaaagtata acagggattg tttaaaaaca cctccaactg aagactgatt
    24421 tttacttcat ctacttaaat gatcaaacaa ttgctttttc tctggtttat catctaaaat
    24481 agagcggaaa aggaggagaa tatcatgcct aaaaatcgtc ttgaagcatt tacagatgct
    24541 gtaatcgcga ttatcatgac tattttagtt ttagaacttc atgaacctaa cggagacacg
    24601 tgggaggcgc tcgctgcatt aggttaccga ttttttatct atattgtcag ttttatcatt
    24661 ttagcgattt attggaacaa tcatcatcat ttatttcaag ttgtacataa gataaatgga
    24721 cgggtcttat gggcgaacag tttctttgtt tttgcgcttt cgctttttcc gtttgcaact
    24781 tcatggatca gtgagtacat caatagcctg gctccagaaa tgacttatgg ttttgttacc
    24841 ttgaccgcta atattgcgta ttatatcttg gcaaaagaac ttgtcaaagc aaataaaggc
    24901 cacgagaaag cattgaaggc gatgttgaat aattacaaga aatcgtatct atcgattggt
    24961 ttgaatgtgt taggaattgt cttagggtat ttagtagcgc cagtagccgt gctgatcgca
    25021 aacatcgtga ttctagctgc ttggatcatc ccaagtcgta ccatcgaatc acaatatcgg
    25081 taaaatgatc cgtgtttaaa atttaagaga aaaatataga aaaaataaat aaacgatcaa
    25141 atatccgagt gatactagta ttcaaagagg gatgcagatg gatcaaaaaa gaattcatgt
    25201 aggactgatt actagttttg tcacgatgct tatttatttg gatcaaggtc atttgacttg
    25261 gacagtagtt ggtgtcgtag gatttttgac attattcaca gcaataaaaa aataaacatc
    25321 tttatgtaat ctgatgttct atctatttaa gaaaagcatg tttacagcag tatgattctg
    25381 ttgtaaacgt gtttttattt gtggaaaatt taatttttag aaaagtgtat cgaaaaatag
    25441 atagttagtt tttccacagt ggataacttc gtaagagata aaaataaaaa attagtcaaa
    25501 gaagggcgta tagatttctt ttgataggag atatacaaca gaaatgaata aatattaacg
    25561 tgttttcaaa cagtataaaa cggtgtggtt tttggaataa aaaaataaca tatctcattt
    25621 tacacttttg ttattttata cttaaaaaat aacgacacag aaaataccgt gtctcagatt
    25681 tctctactac ttctaaaaac tactgttttt ttcctttgaa atgccttcaa aaagtaggat
    25741 caggcagaat atactggcag aatcaaggag aagggagcct aaataaggag aaaaaccata
    25801 gaaaatataa agtaattact tataaaacaa gctttttata gaaaaataat atacctgagt
    25861 agggtataaa ttaagacgga attcctttct agattgagat attgactagg aagaatgtta
    25921 agattttaag cctttaagct aaaaaaaaac cctttttgtt aaataagggg aatatagcct
    25981 gtcagtttca aactgcaagt cgtcctgctg caataatata gtagattaga gatgaaaata
    26041 gattttttta aaccaatgac tgaataaaaa gaccgtcata gatacacatt ggaatgtgtc
    26101 tattacggtc tttttttacg aacgatttta ttgaagacgg acagttcggg tcaaaaataa
    26161 aaaagctacg atcagaaata agaataaaat gtatcccaaa ataatggcta gacggtctaa
    26221 cttatttgtc gatagatgtt tgtttagaag ttgataggcc cataaaccta tcagtcctcc
    26281 tgttgtatta gttatcaaat cagtgatgtc cgctacgcca ataccgaata ggaattgaat
    26341 caattctgcc aagaagctta caaggaaaat aatccctaat tttttagaaa aagaaagtct
    26401 cttgaaattg acattcaaaa gcaccccaaa aggaaaaaag acaatcaagt tataagtggt
    26461 ctcacgtaat accgtcgtat tttcaaaaga aaaagtagaa aaagggacga gattgacatt
    26521 gctgtaagaa taagtgagaa attgtggaag attaaatgat agcttgaata aaatcaacca
    26581 taatagaaag aataaataaa tccctaataa aaattttgat acataccgca tagacatctc
    26641 ctccatttaa aaagtatacc atcgaaaacc gctgaaaaat cataaaaaag tatgaagttt
    26701 aaggaccaac gtagttatcg tacgaaaatt agaagctttt ttttgttggg aatccggttt
    26761 ctttaacaaa gctaagatag cgagttgctt ggaaaagttg ctatcttttt tctgtcaaaa
    26821 cgactcggaa tgttcgtttt ttgttctaat ttctccaagt tgtttacttt ttgatactaa
    26881 gtttatgaaa aatcaagaaa tagttgttat actaggtaat gctgacaaac gggattggca
    26941 tttcatcttg gttcagcttt ttttccaatt actaggaagg agggatagat tgttcaagac
    27001 ttattttcgt ttcaataaaa tacaagataa tccttttcgt atcatgaatc tgatgctact
    27061 tatctttatt gtattatggt taggttacat aaatcatgat tttttgattt caagtttttc
    27121 atcattaggg atctttactt ttttctatta tcaaaacata ccgatgaaat atttgctgaa
    27181 acgtatgttt ctgatcggtt gcggactgtt ggttgctttc gttttgggga tcttatcgac
    27241 ttatgtttta tggcttgaac catttgccgt agctttggtt gctttttcca gcagattcgt
    27301 gttgcgatta ttccatattt ctaaaccagg tggcttgttt tttgcgatgc tttctgccat
    27361 gggaacgagc atgcagctgc caatcgctca attaccgatc gtctcacttt atttttttat
    27421 gggtgtcgtt tttgccctga ttgcggcagt gattacaaaa ttactggatt cacggcctga
    27481 gcagacaatt gaaaaagcaa ctttaaaaga aagatttcac gaagagcctt tagtgatcat
    27541 tgattctgtt ttctacagcg ctgccttatt tctttccgtt tatgtcagtc acggcttgaa
    27601 tctgcataat ccttattggt tgaccttgtc atgtgcatcg attttgctag cagaaaactt
    27661 ggatgcgatg aagcatcgac aagtccaata tctgattgga tcgatgggtg ggttatgtgt
    27721 gtcggcattc ttgtcatttg tgccgtttac gcagttgcag accatttttc tgatcacttt
    27781 tctatatgga atcgcacaat ttctagttgc tagaaattat gcagttgcta atatttttct
    27841 taatccgatg gcgttgatgc tatctacact tattcgtgga gcatacttga tttctttgat
    27901 cgaatatcgt tttttaggga tcgtcatagg aagtttcatc ggtttaggtg ttgcgtgggt
    27961 aatgacggtt ggtttgcaac attatttaac ggttgttcgt caaaaattag aatagaagga
    28021 caaatatatg tgccaaaaga ggttatgaca agatttttgt cacaacctct aattcaaggt
    28081 gttcaaaagc cagattctca gcattaagtc aaattcacac aaaaatgtgg aaaggaactt
    28141 tcaaaaaaat tttatattta ttctatcaaa aaattttgtt tatgatggta actgaataaa
    28201 ctttcctgag atggtcaatg ctttccttga aagtcttaca gatattcttt cttcatagaa
    28261 agtttaagtt ttatagttag tgtacaataa aagtatgttt ataccatatg gtgtccggat
    28321 aatcagtcat ttaaacaatg aggttcaaag atatgaaaag gggaaatttg atgaagaaaa
    28381 taagcaatga aatccaaatt ctggcagaaa ctttaactct tcccaaagtc gttatagagc
    28441 aaatgattgt acaagcaaaa aacatccttg atgaggtgtt tgagcaatat agtcatgaac
    28501 taatggatcc taataaagca aaggcgactg caaagaaaat agacaaagtc tatgaagagg
    28561 aaccttttgc agcattagct atttatctat atgcagcttc tcaaagttgg aaaaaaattt
    28621 atcaaccatt gaagattcca agaactatct atttggcaac aatgaatgct tttactagat
    28681 ttattcatga acatttccaa gtaacaggaa gctatcgctt tgaccgtggt ttttggattt
    28741 ggcattacac tagtggcttg atctttcgga ttggtgaatt ggagtacgaa atgacatatt
    28801 ttccgaaagg gtacacggat ctaagattag aaggaaagca gtgcctttct cttcatattc
    28861 cttccgatgc agatttatct cagaataaga taagagatag ttacgaaagt gccattcgtt
    28921 tttttgaact ttattttcct gattaccatt accaagtgat gtacacagat acttggttac
    28981 tatcacccaa tcttactaaa tggttgaaaa aagagtcaaa aatctgtctt tttgcagccg
    29041 attatcgatt attatcagtt gatgaacagg acgatagtgg agtcccttgg atctttggac
    29101 gagtagatgc ccaaatacat gattatccag aaagtacgag tttacagcga caagcaaaag
    29161 agcaattgct tgcaggcgag cacattggct ctggcttagg tattcttact tcataagttt
    29221 agtacttcga aatagtttaa aagagcgtga gacaaaagta aagaacactt ttgtctcacg
    29281 ctctaaacac gtataaacgg cggaagcaga agcaactcct tcggaaataa gctgaaattc
    29341 acaaaaattt gaaaagcaat tttcgtgaat ttcttcttat ttcttggagt taaacgcttc
    29401 tgtcccagcc tcttttttag tgttctatcg aacacacagt acgttcaata ggtaaactag
    29461 ctataacaat agatattaaa gttaacggac aagagggcct tttttaggta gctaaaacgt
    29521 aataatgaaa atttatttcc agagatagga gtcaatcagc tagtctgatc gactgtttta
    29581 acaagttgac tttcatttta acttttgata ccatttaaaa cacaaatcgt aattgtttcg
    29641 atttgtgttt tttagcatga ctacaatcaa agaagaaagt gggtcgtcca tatggaaaaa
    29701 acagatatct caagtgcata ccgccgcttg aaaagcccaa atatcaaaac acgaaaacgt
    29761 gcgctgaaga tcatcaaaga tgtgaaacgg aatagtggga aaaggtaaga agaaggaaac
    29821 ttaattaaaa aaatagccga aaaacgtttt tcggctattt tcaataatcg gtttttgtgt
    29881 aaggtttccg aaagaactgc tgttgatttt ccatttttaa catatgataa attggtagaa
    29941 tctttctttt caaattgata ccattcccta attccagtta tatagttaac tacagaacca
    30001 caaagcacgt acgtttataa aagtattatc tgtagttatt tttcaattcc ctttcaacct
    30061 cttattttga ccagtagtat tgatcacttt accttttaac acctttgttc gtttttgaaa
    30121 catttttagt caagaatgga aacgtaacta cataatcaaa ggttttgcga aatatttcat
    30181 gaaatatttc atgtagaagc gagcaatacg gttgcaaata accattatcg tttaataagc
    30241 agttacgaaa gctcccaata tctctaacgt tcagctcact ccatttcttt tcttcaaaaa
    30301 aatcagtgat tcgtatctct ttctatttgt cgaaaaagtt aactttaaaa agtaacgccg
    30361 aaaaaatatg caaaaaaata aaaaagcagt tactattgct agaataacat caaggaataa
    30421 taagttttgg ttttcagata atagtcgata agttgtgtat tggaatttta gatacagtaa
    30481 taaagtattt ttcatgaaat tttgtgagaa tcactattac aagtatcttt aataaaatta
    30541 atggttaagg aggaacttaa agtagaatgt aaatgaatag ttgtagtcag tcgattgtga
    30601 tactagactc aaagttgcag gaataagttt ttcaatttgt gattggaaga taaaaaatga
    30661 aaaagaaggg ctttttttta tactttgttg attgtagtgt ttattttccc tctgtttagc
    30721 caagtaacat tttctatgac tcaaccagtt tcaagtgagg aacatgataa cctttttcgt
    30781 gctacagaag agtttaagaa atatcaggaa aatattgttc cggagactcc attagctagt
    30841 gagccaatta ctgatattga tggaaaccaa ataggatggt atatacagta cgaagttatg
    30901 gtggtaaata atgactcaat agtaaaaaat acagtaggct tagagctaac accgattgaa
    30961 aaggtgagtt cattgttaac gtatatttat actgatgaaa gtgacgagac tgagatcatt
    31021 ttaacggatt actcaaaaat tgctactgat aaaaagtata tattgtggat gtagacacta
    31081 gtgaaatact ttatgatata gaaattgatg aaaatttact agatgtagca caagaaatcg
    31141 atcatcaaaa gcaggaagct attcgatatg ctggtagaaa agggaaaaga aaatatttat
    31201 tacgtgaatg tagcaaaagt tagagaagat gaaaatgagt ggaaagaatt caagagtcgc
    31261 tactcaatta actcgactcc gacatttaca gtttacagag aaggatcgat agaaaaaact
    31321 gttttttgga ctaaagaaag tggaatgtct ttagctgaag tagaagagtt tttggattat
    31381 gttagtatgc aacagtaagg gagttcttat tatgaaaaat tttgacacat attatgttat
    31441 tgttttttcg atatctttgc tgttaatatt actaacttta atgcagtata aagagtatcc
    31501 agagtatttt attttaactt ttgtatattt agtaagtagc tacacacttc tatataaatt
    31561 cagaagttct tctagagttt ctaaagtttg tttcttaatt agcttattga ttctataata
    31621 tttttgattt atataggaaa ttaaaaatat tatagaatca acactcagag aacgggttgg
    31681 tttttaatag atttaataag tactgataat gaaatttctc ctggttggga aaacgaagtt
    31741 ggttaccctg tagaagaatt ttttcaagat tactttgcgg agtatgccga taggataatg
    31801 agagattacg taacagataa aactcaagaa gtcatcaatc aatcccagtc attaaatgaa
    31861 ttatctgata aggcgaaaga agaagcagtt gccattattt cttcagggat tagtgactat
    31921 attgatatga gtggtgaaat caattatggc gataaagaca aaattgtacg catgataaaa
    31981 gcttttatca aaacttttga tgtgaaggca gatcttaagt ttaatgatac ttacctagta
    32041 ggaaaattaa ttaatatcct tgggacagag aaagatacgg aaacgtttat ttcctctttg
    32101 tctatgttgt ttacagataa gcatttttca actcagcttg gtggagagga actagtggaa
    32161 atcttttaac cattacgccc agcattaata aaagtatatc aaaaaagtac tcctgatgat
    32221 ttttaagatt ggtttgagaa ataatgtgaa aacactattt ttaatgttaa ttatagatag
    32281 agatgaacgc gtcatatggt taaaagaatg aaaaagtcaa agatacactg ctatatttca
    32341 ctaaggatat agcagtgtat ctttcatgtc gtttcactgg cagtttttta taaaaggagg
    32401 tgtgcatttc agttgctttt ctttggtagt tagttgaatc gtttaagaat taacggatgt
    32461 ggcagacagg tgctctgtcc aaaaaccagt aaaatctttg atggttccat ctttatttag
    32521 ctgtgcctgt tcaaatactg ttttgttttt accgaatgga tcaattgcaa taattgttcc
    32581 gatccccgca cgtttggcag caagtaatcc tgagtaagca tcttcgataa caagacaatc
    32641 agccggagct aagctcagtt tgtctgccgc tttaagaaaa atatccggtt gcggtttccc
    32701 agggaaagag ccgtcgtcat agacaatctt gtcccagtca aaccagcggg ctaaatcgaa
    32761 aatatcaaaa taaaaagcaa cattctcttt gacagtagcc gtggcaatcg ttaatggaat
    32821 ctgttggtca gtcagctggt caagagtcgc ttcaagccct tttgtcaact gaagctcgtt
    32881 ttcattttgc aagcaaagca tacgataata gctttctttt tcttctgaca atacggcgat
    32941 ctcagaggcg gtcaaatgct ctgaaatgaa atgtttcaaa atctccgtat tcgtccgacc
    33001 atgaatattt tttaaaatat cctcgtctga taaaccatct tttgtatgat catgtatcat
    33061 atgcagccaa gcagcttcat gcagatgaga gtccaaaaac attgttccgt tgaaatcaaa
    33121 tataatccct ttcatagtta cgctcctata gtggtatttt cggttgaatc agcaatttgc
    33181 agatcgacat attgtgcata gtgctcttga acctcccgcg agatctggcg atccgtaatg
    33241 agcagatcga aatcatgcaa attataaaac acataaaaat catctcgatt gaatttttta
    33301 ttatcaacga gtagatattt cttacgggaa ttatttagag cgatctgctg tgcttctcct
    33361 tcttcggtac tgtaagtaga gatcgcttcg ttgtgaacgc catttgaact gataaatgct
    33421 tttgtaaact ttagtcttct taaattgtcg ttggtgatcg gtccgacaaa tgcgccggta
    33481 tttttccgga aatcaccgcc aatcagtaga atttctggtc catctgcttg agcaagaata
    33541 tcaaaaactg gataattatt tgtaatgatc cgaattttct tgttcagtaa atgcgtcgcc
    33601 aatagttcga ttgttgtacc tggtcctaag aaaatcgttt ctccctcgtg aatcaaggag
    33661 gctgctgtgg cagcaatttc ttttttttct tgaatctgca gagtggcttt ttcagtgtga
    33721 gaaagttctt gatctaaagt gtaggaaaga ctttgagccc ctccatggac acgcagcagc
    33781 ttgcccgatt tttctaattc atctaaatct ctgcgaacgg tcatatcaga aacatctaat
    33841 tgattgatga tttcattaac ggtaaggatt ccttttttat tgaccatttc tacgatagta
    33901 agcaatcttt cttttttcaa cattgtcttc acctctaatt taaggataat ctatcatata
    33961 aacggacaac agtcaatcta attcgaacaa aagtaaacat ttagggtggt ttttatgttt
    34021 gttacaatag attttattgc tgttttctgg tttttatgat tatttgtgtt tgtttttgtt
    34081 gacaaatgtt ttggatgcgt ttacaatgca atcagttaga aagagcgaaa actgattata
    34141 gatttgagga gagatgtaca tgcttaaatt accagaagat tttatttttg gcggagccac
    34201 agcagcctat caggttgaag gagccactaa ggaaggtggc aaaggcgctg ttgcttggga
    34261 tgacttttta gaggaacaag ggcgattcag tcccgatccg gcaagtgatt tttatcatca
    34321 atatgcaaag gacattgaat tgtgtgaacg ttttggtgta aatggtctaa gattatccat
    34381 tgcttggagc cgcatttttc ctgatggagc tgggaaacca aatccagaag gaatcgcttt
    34441 ttatcatcgt gtgttcgaag aatgtaagaa aagaaacgta acgccgtttg tgacgttgca
    34501 tcattttgat acccctaaac gtttgtttga ccatggagat tttttgaatc gagaaacaat
    34561 agaagcattt gtttcctatg ccattttttg ttttcatgaa ttcaaagaag tcaaggtgtg
    34621 gagtaccttt aatgaaatct atccagttgc aaccaatcaa tatttgttag gtgttttccc
    34681 accaggaatc aaatatgatt ttacaaagat cgtcgcctgt cttcataata tgatggttgc
    34741 tcatgcccgt gtagtgaact atttcaaaga aaacgaactt ccgggtgaaa ttggtgtagt
    34801 tcactcatta gagacgaaat atgcggcaac agacgcgccg gaagataagc atgctgcctt
    34861 tttagacgat gcattgtcga tccgtttctt attagatgct acctatttag gctattattc
    34921 aactgaaacc ttgactgctc ttgatgagat atgtgaagca aatcaggcca gctatcattt
    34981 tcctgaagaa gatttcgttg aattgaaaaa agcatccacg cgtaatgact acttagggat
    35041 caatcactac caatgccatt ttgtcaaagc ttacgatgga gaaaatgcaa tccatcataa
    35101 cggtacgggt gaaaaaggga cctctgttta taaagtgaag ggaatcggag aacgtattta
    35161 taaagaagga attcctcgaa ctgattggga ctggctgatt tatccagaag gtctatatga
    35221 cttgttgctg cgaattaaaa gcgattaccc acattacaac aaaatctata tcactgagaa
    35281 cgggatgggg tataaagatc agtttgaaga tggcattatt atggatcagc cgagaatcga
    35341 ctatttgaga gtctatttgg aatccttaag caaggcgata acagccgggg taaatgtgaa
    35401 ggggtatttt ttgtggtcgc ttatggatct tttctcatgg acaaatgggt ataacaaaag
    35461 gtatggcttg ttttatgttg attttgaaac ccagaagcgt tacccaaaag aatcagctta
    35521 ctggtacaag ttggtcagtg aaaccaaaac gattatttaa taagtgataa tggaggaaga
    35581 aaagatgaat aaagaggaat tacaaatgct tggttttgag atcgttgcat attcaggcga
    35641 cgctcgcagt acattattga aattattgaa agaagtaaga caaggaaact ttgaacatgt
    35701 tgattcggcg ttgaaagaag ccgacgaaaa tttaaccctt gcacataatt cgcaaacaaa
    35761 aattttagcg gaagaagcat ctgggaaaga gatggagatg ggattcattt ttatccatgg
    35821 gcaggatcat ttaatgacca ctttattgct gagagatctg attcaagact ttattgtact
    35881 gtacagacag aatcaaggat aggagaagaa cgctatggat ggaatcatta aacaaatcga
    35941 aaaagggaaa ccattttttg aaaaaatctc acgtaatagt tatttaggag ccgttcgtga
    36001 cggattttta acagcgatgc cagctatttt attttcaagc attttcattt tgattgctgc
    36061 ggttcctgag atttttggtt accaatggcc tgaatctgtc tccacatggc tatggaaagt
    36121 ctataattat tcgatgggtg ttgtagggtt actcgtttca gcaaccactg ctcgctgctt
    36181 ggctgattcg atgaatcgtc gcttaccgaa aaacaaagta atcaacacca cctctgtgat
    36241 gctggcctct gtggtaagtt tcctgttgtt aagcgtctct ccgattgaag gtggattttc
    36301 aacggaatat atggggacaa agggactact ggcttccttt gtcgctgcat ttttaacagt
    36361 taatttatat aagttttgtg tgctgaaaga tattaccatt aaaatgccta aagaagttcc
    36421 tggaacgatt tcgcaaacct tccgagatat ttttcctttc tctttttcag tatttgcggc
    36481 cgttttaatt gatttaggca ttcgacaaat cttccaaatc tcatttgcag aagcaatcat
    36541 tacggtgctg cagccgctat ttacagcagc tgacgggtac ttaggaatcg ctattatctg
    36601 gggagcgatg gcgctgtttt ggtttgtcgg tgttcatgga ccgtcgatcg tggagccggc
    36661 aattgcggca attatctatg ccaatgtgga aaccaatctg caattgttca attccggtga
    36721 acaagcttct aaagtactga cggtgggttt agggaatttt gtaggaacta tgggaggaac
    36781 tggggcaaca ttggttgttc cttttatctt cctactcttt gctaagtcta agcagttgaa
    36841 ggcagttggg aaagcttcct ttattccagt aagtttcgct gtcaacgaac cattgctatt
    36901 tgcggcaccc atgattttga acccttattt ttttattcca tttttgttaa cacctgttgt
    36961 aaacgtttgc ctgttcaagt tttttgtcga tgtattgggc atgaacagct ttatgtacgt
    37021 tttgccttgg gcaactccag caccgatcgg tttggttcta ggaacgggca tgggtatctt
    37081 agcttttgtt ctagcagcgg ttctaatcgt cgttgacttc ctgatctatc taccattttg
    37141 caaagcatac gatgctgtct tagtagagaa agaagcacaa aaagcagagg tgatcgcaac
    37201 cgaggaaggt gcttctgccg atgtacgtga ggaagtacct gtggcagcga gtcaagttat
    37261 cagcgatgca aataaagaaa tcaatgtgtt ggtactttgt gctggcgcag ggacaagtgc
    37321 gatgttagct aatgctttaa aaaaaggtgc atctgaatcg aaagcaccga tcagtgcaat
    37381 ggcgggtgct tatggttctc attacgaaat gctgcaggat tttgatatgg tggtcttagc
    37441 accgcaagtc aattcttact atgatgatat caaagaagac accgatcgct taggaatcaa
    37501 gctggttgcg acaaaaggag cagaatatat cggattaacg agagatcctg aaaaagctgt
    37561 tcagtttatt ctgaaccaat ttcaataaag gaggagccat aatgaaaatg ggaatttggt
    37621 tgcgtaaagc tgttttattt gcagctgtgt taggggcttt gggggctgtt tcaaaaactg
    37681 ttgaagcatc tgccagcgaa tttattcgcg gagcagatat ttcgatcctg tcagatatgg
    37741 aaaaatctgg tgcaaaatat tatgaagacg atatttcaaa agatgcctta gagattttaa
    37801 aaaataatgg tgtcaactat gttcgactgc gtttgtggca ggatccttat aatagtaatg
    37861 gagaaagtta tggagcaggg acaaatgatc tggaaacgac gattgacctt gcaaaacgag
    37921 caaaaaatct gggacttaag gtgctgctgg attttcacta cagtgatttt tgggttgatc
    37981 ctgggaaaca gaatcttcct aaagcatggc aaggtctgac ctttgaagaa atgaatacgg
    38041 cattgtacga ctatacaaag aacgttctgt cggaaatgaa gcagctggac gtttatcctg
    38101 atatggttca gatcggcaat gaactgaata gcggaatgct ctggccttat ggcaagtctt
    38161 ggggagaagg cgggggagaa tttgatcgat tggctgcttt tttgaagtct ggtatccaag
    38221 ctgttcgaga cactcaacct aaaacaactc cagtaatgct ccacttagct gatggtggcg
    38281 atacaggagc atttacttgg tggtttgatg aaatcacgag tcgaggtgtg tcatttgatt
    38341 taattggagt gtcgtattat ccgtattggc acggttcaat gaatgaactt caaaccaata
    38401 tgaatgcgat cagccaacgg tatggtaaag aagtgatcgt agttgaaaca gcttatgggc
    38461 atacaacagc gaatgccgat actatgccaa atgcttttgg tgaagccgaa gcagctgcgg
    38521 ggggatatga accaacaccg acaggacaag cagagtattt gctggatctt gccgatcgga
    38581 ttcaggcagt accgaataat cgcggggcgg gcttcttcta ttgggaacct ctttggtaca
    38641 acggaaatgt gagttgggca acacaagcag gtatgaatta tctcagtgtt caatccacta
    38701 tgggaaacga atgggacaac caagcgatgt ttgattttgc tggaaacgcc ttgccaagct
    38761 tgcgtgcgtt taaacaagca ggggcgcaaa taaatctagt taaaaatgca agtttcgaaa
    38821 gcgatggacc aacgaccagt ccttccaatt ggcaaacctg gtttcaacaa ggaaccgcta
    38881 agggtacgat caaaacggaa gcgggcactt tttttgggga ctacaaacta actttttggc
    38941 aggatcaagc atacgaggca tcagcttatc aaaatgttag tggcataagc aaaggaactt
    39001 acacattgag tgcctatgta atgtctgggg gagagtttga tactaatcag ttatatatca
    39061 aaaattatgg tggagcggaa ctaaatactg cggttgttaa aacaagtgaa tggacaaaaa
    39121 ttacaatcaa agacattcca attacaacag gtgtttgtga aattggggtt tatagcaagg
    39181 cttcaggaaa tgcttggacg aacgttgatt ttgtttcatt ggtgaaacaa tagactgcgg
    39241 atcgtttcga ttcaagatcg gaggttccct tcagccacat cgttagcgta gaaggcagga
    39301 atcgaacctg cacctcgcct ttgccaaaaa taaccagaaa cgatttccag tcatttttac
    39361 caaaacgagt aaagcaccag acttcatcta cactttgtat tatgctaaag agagttcaac
    39421 gatttcgtaa gaagcaatga aaaaattgca aaaagttgtg cagataacat aaagtttgtt
    39481 taagaaaggg taaccttaga attccgtcgt gaatatgatt tgagcactta gatggataga
    39541 aaaaagttct cggaaaacag ttcgtttttc gagaacttct ttttatgata agaatgaaac
    39601 aaattaactt atattatagt ctcttttggt agggaaccat cttattttta atgaaaatca
    39661 agcattttag ctattgaaga atgttgattt atagggattc cccctccata ttatgttggc
    39721 tctttattcc cactccactg ttgctggtgg tttagaagtg atgtcataca cgatacggtt
    39781 gacgtgggct acttcgttta cgatacgtac agagattttt tgcaagactt cccatgggat
    39841 acgggcaaag tcagcggtca ttccgtcgat tgaagttaca gcacggatac cgactgtgta
    39901 gtcgtaagta cgtccgtcac ccataacacc gacagagcgg atccctggaa gaacagtgaa
    39961 atattgccag atatcacggt caagtccagc agctgcgatt tcttcacgca agatagcatc
    40021 agattcgcgt acgatttcta gcttgtcttc agtgatttcg ccaagaacac gaataccaag
    40081 accaggacca gggaatggct gacgccaaac gattgaatca ggcatgccta actgagtccc
    40141 taattcacgg acttcgtctt tgaataatgt gtttaatggt tcgatcaatt caaattgcat
    40201 atcttctggc agtccaccta cattatggtg agatttgatc gtctgtgctg tttctgtacc
    40261 agattcgata acgtcagtat acaacgttcc ttgtgctagg aaagagacgc cttcttcacc
    40321 agcaagtttt gttgcttcgt catcaaaaac atatacgaat tcattcccaa tgattttacg
    40381 tttttgttct gggtcagata cacctttcaa cttgtctaag aagcgatctc ttgcattgac
    40441 tttgatgatg ttcaagccga attttccgcc tagactttcc attacttgtt ccgcttcgcc
    40501 tttgcgtagc agaccgtggt caacaaagat agatgtcaat tggtcaccga tcgctttttg
    40561 taaaagaacc ccaacgacac tagaatcaac gccacctgaa agaccaagca agactttttt
    40621 gtcaccaact tgttcacgga ttttttctac ttgcatatcg ataaagttat ccattgtcca
    40681 gttaccttca cagtgacaaa catcaaaggc aaagtggcgc agcaattcga ttccgtattc
    40741 ggaatgacgt acttctgcat ggaattggat accataaaaa ttacgtgttg tgtcttggat
    40801 agaagcaatc ggacaatctt tgcttgttcc gactgtttca aagccttcag gaactttagt
    40861 taccaagtca ccatggctca tccatactgt ttgttgtttt ggtgtttctt taaacaagac
    40921 agcttgatcg cttgttactt ctaacaaggc tttcccatat tcgcggtttt tagcaggctc
    40981 tacggaaccg ccaagtttgt acgtgatcaa ttgcatacca tagcaaatcc ctaatacagg
    41041 aatacctaaa ttgaagatct caggatcgat cccaaatgca ttctcatcat atacactgtt
    41101 tggtccgcca gaaagaatga ttccctttgg attcatcgct tttacttctt ctgcagtaat
    41161 gcggtggctc aataattcgg aaaagactcc aagatcacga atacgtcttg tgatcaattg
    41221 gttatactga ctaccgtagt ctaatacgat aatcttttca acgttcgtca tatcggcaac
    41281 gtttgtcaaa tttattaccc ctatcccttt aaattttatc tcaaaatagt caaactattt
    41341 taaaatgcta atattttcgc atgaaaatcg aatcgatgat atgattttca gttttatgga
    41401 gaattatatc ggctcttcct cgtgtaggca agatatattc ctgtaaattt ttcaaattca
    41461 catttttcca tacatctttt gccattttaa aggcttcttc acgatctccg atagcaaaac
    41521 gataataata attctctgga ttttggaaag ccgtatccag taacgcacca aaacgatcca
    41581 aataccattt ttcgatcagc acgggatcgg catcgacata gacagaaaaa tcaaaaaagt
    41641 cgctgacgta gatttgctga ttcgcaggta attgaagcgt attgatcccc tcaacgatca
    41701 agatatcagg ctgctggatc aattcgtact tgtctggaat gatatcatac acatcatgtg
    41761 agtaaacagg tacttttatc tcttcctgac cagattttac acggttcaag aaatcgatca
    41821 gcatctccat atcataactt tcaggaaatc ctttacggtc catgattcca cgctctttta
    41881 atatacgatt tggataaaga aatccgtcag tcgtgatcag ctgaacgttt cttcgtttga
    41941 aggtacgaga caaaatcgtc tgcagcagtc tggctgttgt actttttcca acggctacac
    42001 ttccggcgat cccgataata aaaggcggta cttctacgta tttatgaaga aataaacctt
    42061 tactcaagaa caaagattca aattctttca tataaagata aatcagatga gcaagaggta
    42121 catagatatc tcgaacatcc tgcatggaaa tgcggtcatt caaacttttg atattatcta
    42181 gttcatcttg tgtcaaaggt gcttgcccat tacgataaaa ttctcgccat tcttctcttg
    42241 ccacacggta gtagttcatt ggatcattca tattagcctc ctgcgttctc ccatcaatgg
    42301 aactatttta acacaaaata aacgcaaacc ccaccactta agagagattg ccgatctttt
    42361 tgtataatct ttataataga aagaaacgga gtcttattct tgacttgcgc acggatacat
    42421 aaaaggatag actgttgtca tgaaagaaat gagaaggatg gaagacatga caaatcatta
    42481 ctattctgaa cgcccagata cggcgcacga ttttgaacaa tgggcttttg agttaaaagg
    42541 cagaaccttt caatttgtaa cagattctgg tgtattttca cgtgaaacag tggattatgg
    42601 ttcacgtgtc ctgattgatg ctttcgattg ggaagagtta cctgatgaag gaaagatctt
    42661 agatgtggga tgcgggtatg gaccgattgg gttagctttg gcatttgcga cacagcggtt
    42721 tgttgaaatg gtcgacatta atgaacgagc ggtaagtcta gcgcaaggaa acgcaaagcg
    42781 taaccaaatc gaacatgttg atatccatca atcgaatatc tatgaagcag ttcatgaaga
    42841 aacctatgca gctatcgtaa gcaatccgcc gatccgtgct ggaaaaaaag ttgtacatga
    42901 aattttgaca ggcgcatatc ctcggttgaa gaagggcggt acactgacaa ttgtgatcca
    42961 gaagaaacaa ggagcgccga gtgcgcaaaa gaaaatggaa gaaacatttg gtaatgcaga
    43021 aatcgtcaca aaagataaag gatattatat catcaaaagt gtgaaagaat aactagctga
    43081 aaaatagtgg agcgacgaaa gagtggaact gggcgtgcat gtacgggtac gtatcagttc
    43141 tttttttgtt gagaagtttt taaaaacaaa gaactatttt ttcttcaaaa agaaatagta
    43201 ggaacagagt cgttgttttt tacgataaaa taaataagat acagaacttg tatagaaggc
    43261 tgccaagtcg gtagataaag gaggttttta tcaaaaaaca tactttcctt ttttctctat
    43321 tcttttcttg ggggttttgg tacactgata gggaatttag atagaaggga gccaatagga
    43381 tgaagctgac catcagagat atcgcagaaa tggcaggcgt atcagtaaca actgtctcac
    43441 agattttgaa taacaaagga agcagattta gtgataagac aagaaaaaaa gtgttgtcga
    43501 tcgtgaatga acatcattat aagcccgatt atttcgcttc aaatgtgatc aatcgacatt
    43561 caaagaccat cggaatgatc gtccctgatg tcactgattt cttcttctcc aaactgatcg
    43621 aaggtgtaga gagctatttg aatcctttgg gctatgtgat catgttatgc aattctcgtc
    43681 atagccaaga aaatgagatc aagtatttac aagagctttc ccaccgctca gtggacggaa
    43741 tcttgctggc cacaccaaat attttacctg agaaatatgc attagacagc ggtttttatc
    43801 aaaaaatgcc aatcatttta atcgatcgcg gattgaatcg tcgggataac ggacggctta
    43861 tcgtaaagga atatgagggc gcctatcaag ctgtttcgct gtttattgaa aatggccata
    43921 cgaagatcgg catgctgaag gaaaccactg gatactatca attagaagaa cgtttcaacg
    43981 gatatcgtca tgcgctgaaa gatcatggcc tgccttttaa tgggaaattt gttgaacatg
    44041 gagagttgac tgtccaaggt ggatatactg catcaaaaaa attactcaaa cataaagata
    44101 tcacggctat tttttgcggg aatgatgcca tggcgatcgg atgctatcag gcgatcgatg
    44161 aattgggcaa aaaaatccca gaagatatct cagtcatagg attcgatgga ttaaagctgt
    44221 cagaatacat gattccgcaa ttgacgacag tgcagcagcc aagttttgat attggatttt
    44281 atgctgcacg tttcctgatc gatacgatcg aatttcctca aagaaaagtt ccgaacaaag
    44341 tgttcgaaac aaaattaatt attcgtgaga gcgtaaaagt attgacaaag ggctaactcg
    44401 gcggtatatt gcttatgtaa gcatttacaa aggctagtta gtgctttttt tatttttagt
    44461 aaaccagttt actaaagtgg tttacctttc ctaaaaataa tgtataatac cgaatgaaga
    44521 caaggaggat caggcaatgc gaatggtgga tctgattgaa aaaaagaggg atggaaacga
    44581 gctgtcgaaa gaggaaatcg agtacatcgt cacgaactat acgaatggaa agattccaga
    44641 ttatcaagtc agtgcattat tgatggcaat tttttatcaa gacatgacaa atgaagaaat
    44701 cacgaactta acattggcga ttgctaattc tggtgatgtc atcgatttga gttctcttga
    44761 aggaataaag gtagataagc attcgacagg tggcgttggt gacacgacca cattgatttt
    44821 agccccccta gtagctagtg tcggagtgac agtggctaaa atgtctggac gcggtctagg
    44881 ttacacaggt ggtacactag acaagctaga agcaattccc ggatttcaga tcgagctttc
    44941 ggatgaagaa tttgtacgca ttgttaatga aagtaaagta gcggtaattg gtcaatcagg
    45001 aaacttggca ccggcggaca aaaaattgta tgccttgcgt gacgtcacag cgaccgttga
    45061 ttctcttccg ctgattgcca gttcgattat gagtaaaaaa atcgcggcag gagcagatgc
    45121 aatcgttttg gatgtgacca ctggagacgg tgctttcatg aagaacatcg aagatgcaag
    45181 acgtctggca aagaccatga cgagcattgg gaaactggct aaccgagaaa cagtggcggt
    45241 tatctcagat atgtcagaac ctcttggcga agctatcgga aatagtctag aagttgtcga
    45301 ggcaatcgaa acattacaag gaaatggtcc agaagatcta gtagaaatgt gctacgcctt
    45361 aggaagccaa atggttgttc ttgctggcaa agctaagaca accgatgaag caagaacgtt
    45421 gcttcaagaa gctctggaat caggtaaagc gttagcaaaa ttcaaagaaa tgatccaaaa
    45481 tcaaggggga gacccagcga tcgtggaaca tccagaacga attttgactg cacgctacac
    45541 aatggagctt cctgctaaac aaagcggagt tgtgtcaaaa atcgtggcaa atgaattagg
    45601 aattgctgca atgatgctag gtgctgggcg taaaaccaaa gaagaagata ttgatcacgc
    45661 tgttggattg aaactgcata aaaaaattgg tgacactgtg acaaaaggtg agtcactatt
    45721 gacgatctat agtaacgacg aagaaatcac ttctgtcatt gaattattat ataaaaatat
    45781 tgaaattggt gaatcggcaa tgaagccaac gttgattcat gacatcatca cagaataaag
    45841 gaaaaaagcg gactttaccg ttttaaagaa aaggggaaaa gaaatggaac taaaccgaat
    45901 gatcgaccat actattttga aagcagacgc atctaaagaa gctgtgatgc aaattatcga
    45961 agaagcaaaa aaataccatt tttattctgt ctgcatcaat ccagcatggg ttgcgctagc
    46021 gaaagaacaa ttacaaggtg agccagtagc cgtatgtaca gtaatcgggt ttcctctagg
    46081 agcaaacaca cccgagacaa aagcatttga aacaacagat gccatcaata acggtgcaga
    46141 tgaagtcgat atggtcatca atatcggtgc tttgaaatca aaggatgatc aacaagtcca
    46201 aaaagatatc gaagcagttg ttgaagcagc aaaagatcgt gcgcttgtca aagttattat
    46261 tgaaacatcg ttattagata gagaagaaat catccgagct tgcgaaatag caaaagctgc
    46321 aggcgctgat tttgtcaaaa catcaactgg tttctcaaca ggcggagcaa aggtagagga
    46381 tgtccgtttg atgcgtgaaa ctgtaggacc agaaatgggt gtcaaagctt ccggcggtat
    46441 ccataacgaa gaagaagcaa tggcaatgat cgaagctggt gctacaagaa tcggaacaag
    46501 cgcaggagta gcaatcgtat ctggttcaac tggtgaaggc tattaatttt ttcaaaggag
    46561 aagctcatgg cagaactcaa acaagaatgg atcaacacag ctattgaagc gttagataaa
    46621 gcctatgttc cttattccca ttttccggta ggcgcttgtc tcgtaacgga atcaggcaag
    46681 atttatcaag gcatcaacat tgagaatgca tcatttggat taacgaattg tgccgagcgc
    46741 acagcttttt tcaaagcagt atctgaagga gaaaaaacat ttacgcatct agtcgtagcg
    46801 gggcacaccc ccgatcctat ctctccttgt ggtgcatgtc gacaagtaat ggcagaattc
    46861 tgttcccctg atatgccggt tacgctagta ggagacaatg gtgtgacgaa ggcaacgacc
    46921 gttcgtgcac tgctgcctta tgcatttact gaaaaagatc tataactcat gttcattcag
    46981 tcagctgact gttgaaaaat aaaacaatta ttttaggagg ctttacagaa atgaaaaaag
    47041 caaaattatt tggtttaggt gctgtcgcac tagcagcagg gttattttta ggtgcatgcg
    47101 gcaacaacgg ctctactgat tcatccggtg gcaaaagctc atcagacaca acaactgcag
    47161 cattgatcac agacacaggt ggtgtagacg accgttcatt caaccagtca gcttgggaag
    47221 gactagaaaa atgggggaaa gatcaaggac tttcacgagg aaatgatggc ttccaatatt
    47281 tccaatcttc aaatgaatca gattacatcc caaatattga ccaagcattg aatgctggat
    47341 tcaaaacgat ttttggtatt ggttataaac taaaaccagc gatccaagaa caagcaacaa
    47401 acaatacagg tacgaacttt gttattatcg atgatgtcat cgatggatta gataacgttg
    47461 tatctgcaac tttcaaagac aacgaagctt cttatcttgc aggtgttgca gcagcttata
    47521 cgactgaaac aaacgtagtc ggctttatcg gcggtgtaaa aggtgaagta atcgatcgtt
    47581 ttgatgctgg attcaaagcc ggtgttgatg caggtgcgaa agaattagga aaagaaatca
    47641 aagtattgaa tcaatatgct ggtgacttct ctgctccaga taaaggacgt tcaattgctc
    47701 aaggtatgta tgcacaaaac gcagatatca tcttccatgc ttcaggcggt acaggtaacg
    47761 gtgtattcca agaagcaaaa tcattgaacg aatcaggcga caaaaaagtt tgggttatcg
    47821 gtgttgaccg cgaccaatca gacgaaggcg aatacacatt aaacggcgaa aagaaaaact
    47881 tcacattgac atctacactg aaagcagttg gaacagtagt agaagatttg gcacaaaaat
    47941 cagcggacgg taaattccct ggcggcgaac acactgttta tggattgaaa gaagacggtg
    48001 ttggtctaac agaaggacaa ctttctgacg aagcgaaaaa agcagttgat gaagcaaaag
    48061 aaaaaatcat ttcaggtgac gtaaaagttc cagaaactcc tgaagaaaac taaaaccatt
    48121 tctatagcct tgttagacac atgtttgaca aggctatggt ttgattagaa caagaattgt
    48181 tcatttatcc aagctgcctt gtgacagttt ggataaggga acaactccct gaagtaatgt
    48241 ttgtttcaaa agaagaacgc tcactaagcg tgaatcttag tcaatcgttg ccctagatag
    48301 aaatcgttag gatgtgaaaa aaaggtggtt caagaaaata ttgtcataga aatgcagaat
    48361 attaccaaag aatttggaac attcaaggca aatgacaaca tcaatttaca agtaaaggca
    48421 ggagaaattc atgcgttgct tggagaaaac ggtgctggca aatctacatt gatgaacgtg
    48481 ctttccggat tattagagcc gacatcaggg aaaattttga tgcgtgggaa agaagtaaag
    48541 atcacaagtc cgaccaaagc caaccaatta gggattggga tggtccatca gcactttatg
    48601 cttgttgatg cctttactgt aacagaaaac atcgtgttgg gaagcgagcc tagtcgcgca
    48661 gggatgcttg accataaaaa agcacgaaaa gagatccaaa aagtttctga acagtatgga
    48721 ttgtcagtca acccagatgc ttacgttcgt gatatttcag ttggaatgga acaacgggta
    48781 gaaattttaa aaacacttta ccgaggagca gatgtactga tttttgatga gccgacagct
    48841 gtattgaccc ctcaagaaat tgatgaatta atcgtgatca tgaaggaatt agtcaaagaa
    48901 ggcaagtcga tcattttgat tacgcataag ttagatgaaa tcaaagcagt agctgaccgc
    48961 tgtacagtta tccgccgtgg aaaaggaatc ggtacagtca acgttaaaga cgtaacctca
    49021 caacaattag ccgatatgat ggttggaaga acggtttcat tcaaaacgat gaaaaaagaa
    49081 gcgaagcctc aagaagtcgt tttgtctatt gaaaatctag tggtgaaaga aaatcgtgga
    49141 ttagaagccg tgaaaaactt gaacttagag gttcgtgctg gcgaagtact tggtatcgct
    49201 ggaatcgatg gaaacgggca gtcggagttg atccaagctt tgactggttt gcgaaaggca
    49261 gaaagcggac atatcaagct aaaaggggaa gacatcacta ataaaaaacc ccgaaaaatc
    49321 actgaacatg gtgtaggaca cgtgccagaa gaccgtcata aatacgggtt agtcctagat
    49381 atgacattat ctgaaaatat cgcactacaa acctatcatc aaaaacctta cagtaaaaac
    49441 ggcatgctga attattcagt aatgaatgaa catgccagag aattgatcga agaatatgat
    49501 gttcgaacaa cgaatgaact tgttcccgca aaagctttat caggcggaaa tcagcaaaaa
    49561 gcaattatcg ctcgtgaagt cgaccgagat cctgatctgt tgattgttgc aaatccaact
    49621 cgtggactgg atgtaggtgc gatcgaattt attcataaac gtctgatcga acaaagggac
    49681 aaatacaaag cagtgttact gatcagtttc gaattagaag aaattttaaa tgtttcggat
    49741 cgtattgctg ttatccatga aggagaaatt gtcggtatcg ttgatccgaa agaaacatct
    49801 gaaaatgaat taggtctatt gatggcgggc tataccttag aagaagcacg aaaagagtta
    49861 gaaaaggggg ctggcgcaca tgaatgatcg cagcgaaaaa ttccgtaatt tattagtgcc
    49921 tgtactttca gttgttttag gattgatctt aggcgcgata ttgatggctg cttttggttt
    49981 tcagcccatc caaggttatt cttctatgct agatgcagcc cttggttcgc aaagagggat
    50041 aggtgaaacg ttaaggcaag caacgccgtt gatttttaca gcattaggtt tttctgtagc
    50101 taactcagct gggtttttca atattgggct ttctggacaa gcactttgcg ggtggatcgt
    50161 cagtgtctgg gtggcacttg cgtttccaga tctgccaaaa gttgttttgc ttccgttatg
    50221 cgtgatctta ggtgccttag ctggtgcgat ggcagcggct gttccaggtt tacttcgagc
    50281 attttttgga acaagtgaag tgatcgtaac aatcatgatg aactatatct tattatacgt
    50341 aagcacattt gctttacatg aagtgatgcc tgaaagctat cgctcaagtt tggactcttc
    50401 caatatgatc agtcaaaatg cctcactgcg gttaccatgg ttaacacaag cttttggcgg
    50461 ctctcgcgta aactcaggat tatttttagc aatcatcgcg cttattttcg tttggctgct
    50521 gatgaagaaa acgactttag gatttgaaat ccgttctgtt ggattgaatc cttttgcttc
    50581 tgagtatgca ggaatgagta gtaaacgtac gatcgttttg tcgatggtca tttctggcgc
    50641 tttggcaggg cttggcggtg tggttgaagg attaggaacg tatcaaaact tctttgttca
    50701 aacgacttcg ctatctattg gatttgacgg aatggctgtt tccttgcttg gttctggatc
    50761 agcaatcggt attcttttat cagcgatctt gttcagtatt ttgaagatcg gtggacttgg
    50821 catgcagaca ggagcaggtg ttccgtttga gatcgtcaat gtatcgattg ctttgattat
    50881 cttctttgtc gggatcaact tcctgattcg tttcgtcatg gctaaagtct tccaagggaa
    50941 aaaacaagaa gagatcgttg cgacaataga atcgaaacca acgaatcaaa accatgaagg
    51001 gggcgaacta taatgtcgac tatcgaatta attgccttga tccttacttc tacactggtt
    51061 tattcaacac cgctaattct aacgtcacta ggcggaactt tttctgaacg aagcgggatc
    51121 gtaaacgtag ggctagaggg aatcatggtc atgggtgctt ttagttccat cgttttcaac
    51181 ttgacgatga gtccatcact tggttctatg acaccatgga tcgctatttt agtgggtggc
    51241 gtcgtaggta tcctattttc tctgattcac gcagtagcaa cagtcagttt gcgtgctgac
    51301 catattatca gcgggacagt aattaatctg atggcacctg ctttaggtgt tttcttgatc
    51361 aaagcatggt atggtaaagg tcaaactgat aacatcacgc agaatctggg gtatttttct
    51421 ttcccaggat tagcagatat tccggtcatt ggaccaattt tctttaaaaa tacattctta
    51481 cctgcttatt gtgctatttt gattgctgtc ctagcttggg tgatcatctt caagacgaaa
    51541 ttcggcttgc gcctccgttc agttggtgaa aatcctcaag ctgccgacac attaggtatc
    51601 aatgtatatg ctatgcgtta ttcaggcgta ctgatttctg gcttgttagg cggtatgggc
    51661 ggtgctgttt ttgctcagtc gatttccggt aacttttcag ctggtacgat cgttggacaa
    51721 gggttcatct ccatggctgc tatgatcttt ggtaagtgga atcctttagg ggcaatgggt
    51781 gcctcattgt tctttggttt tgctcaaagc ttgagtatca ttggcgcaca attaccagtt
    51841 atttcttcga ttccgccagt attgctgcaa attgctccat atttgctgac aatcattgtg
    51901 ctggttgttt tcttagggaa agcttctggt ccaaaagcaa acgggaagaa ctatatcaaa
    51961 tcaaaataat cttttagcga ataaaaaggg tgggttacgg gaagtgtttt tcgtctcgcc
    52021 ctttttgttt taaaggcaat aaaaataaaa agaaagaagg aataatcatg tttaaacgcg
    52081 tacatctgat cgtcatggat tcagtaggaa ttggagaagc accagatgcc gataagttcg
    52141 gtgatgttgg ttctgataca ttaggacaca ttgcgaaaga agcaggattg aacgttccac
    52201 atttagagca gcttggttta ggaacgatcc gtcctcttga aggtgtggaa tcagtgaaag
    52261 atcatcaagg ctatgcgacg aaattagaag aagtttctgt agggaaagat acgatgacag
    52321 gccactggga aatcatggga ttgaatatca aaactccatt tcgtgtgttt cctaatggat
    52381 tccctgatga tctgatcaaa caaatcgaag aattttctgg ccgtaaagtc gtatgtaaca
    52441 aaccttatag cggaacagct gttattgacg attacggtga acaccaaatg aaaacaggcg
    52501 atttaattgt atatacttct gccgatcctg tccttcagat cgcggcacat gaagaaatca
    52561 ttcctttaga agaattatat cggatttgcc aattcgttcg tgatataaca aaagatgatc
    52621 cgtatatgat tggccgaatc attgcacgtc cgtacgtggg tgaaccagga gacttcacac
    52681 gaacaagcaa tcgccatgat tatgcgttga atccattcgg acacacagtc cttgattctt
    52741 tgaaagaagc aggaaaggac gtgattgctg ttggaaagat caacgatatt ttcaacggac
    52801 aaggaatcac tgaatctgtc cgcacgaaaa gcaatatgga tggcgttgac cagctgctga
    52861 aagttatgaa aaacgatttt tcaggaatca gctttaccaa tttagtagat tttgatgcat
    52921 tatacggtca tcgtcgggat gtagtcggct atgcgcatgc gattgaagaa tttgacttac
    52981 gaattccaga attgcttgaa gcgatggaag aagacgactt gctaatgatt acagccgatc
    53041 acggaaacga tccgacgttt actggtaccg atcatacgcg agaatatgta ccgttattgg
    53101 tttacagtaa aaaaatgaaa gaacaagggg cattgcctca aggatattat tcagatattt
    53161 cagcaacgat tgctgagaac tttagtgttt cttcgactga aaatggcgaa agcttcttga
    53221 aattattaaa ataatctttt taaataggag aaagcaaatg accaagttaa gcgagatgtt
    53281 acaagaaacg acccagttta tccttgataa aggagtaaaa gaaattgatt ttggtttgat
    53341 tctaggttca ggattaggtg aattagcaga tgaaatcgaa gatgctgtaa tgatttctta
    53401 tgatgagatc ccgttctttc ctgtttcgac agtagttggg catgcaggaa agctagttta
    53461 tgggtctctg gcaggcaaga aagtgttagc catgcaaggg agattccact actatgaagg
    53521 tcattccatg caaacagtga cctatccagt acgtgtgatg gccggactag gggcccattc
    53581 agtcattgtg actaatgcat gtggtggtgt caacgaatca tttgtaccag gagatttgat
    53641 gctgattacc gatcatatca attttactgg tgaaaatcct ttgatcggtc cgaatgaaga
    53701 tgcaatgggc cctagatttc cagatatgag tcaagcttat acgcctgact atcaagcaac
    53761 agcaaaagca gcagcagaaa aactaggttt ggatcttaaa gaaggcgtat atatgggcta
    53821 ttctggacca acttatgaaa cgcctgcgga gatccgaatg tctcggacaa taggagccga
    53881 tgcagtaggg atgtcgactg ttccagaagt catcgtagca gctcacagcg gattaaaagt
    53941 gttaggaatc tcttgtatta ccaacttagc agcaggtatg caagcgaatc tgagccatga
    54001 agaagttgta gaaacaactc agagagtcaa acaatctttt aaagcattgg ttaaagaaac
    54061 attgacatta ttgtaatagg aggaacaata aaatgagtgt gcatattgaa gcgaaagaag
    54121 gcgagatcgc cgataagatt ttacttccgg gagatccttt gcgagctaaa tatattgcgg
    54181 agactttttt ggaagatcca gtttgttaca atcaggtacg cggtatgtta ggctttaccg
    54241 gcaaatataa aggagaacgt atttctgttc aaggaacagg aatgggaatg ccatcagcca
    54301 ccatctatgc ccatgaattg atccagtcgt atggtgtaaa gaaattgatt cgagtaggta
    54361 catgcggtgc attgtcaaaa gacgtccatg ttcgtgattt agtactagca caaggtgcag
    54421 cgaccagttc ttcgatgatc gaaaaaaatt tccaagcctt tcattttcct ccaatcagtg
    54481 atttcaatct attactgaaa gcatatgaga tagctaaaga aaagggctat acggttcatg
    54541 taggaaatgt tctttcggaa gattctttct acaaagatga tttgacagag acgttccaat
    54601 tagctgaact tggcgtttta ggagtggaaa tggaagcagc ggctctttat tacttaggcg
    54661 cgaaatacca tgtgcagaca ttaagtttga tgaccgttag cgaccatttg atcaccggtg
    54721 aagaaacaac agctgcagaa cgtcaatcta cgttcaatga aatgatcgaa gtaggattgg
    54781 aaacagcgat agcagaataa gaaggaaatg aaaaagctgc cctgcattaa gtaataagaa
    54841 catccaagca aaaatagctc ctcatgtttt tgacttggct ggacttatta ttgcaaatgt
    54901 aagcttttga acaccgttta ccaagaggtt gtgaatctgc cgtttaactc tgagtcacaa
    54961 ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg tgaccgagaa
    55021 gttggcagga gaaaaccgtt tatacgaaaa aagggactgt gacaaatcgt gtcacagtcc
    55081 cttttttata gtgtttttta taggtagtat tttttagcaa ctgttaagtc atcatttaat
    55141 tcataaacaa gtggttgacc tgttggaatt tcaagatcca tgatgtcttc gtcagagata
    55201 ccttcgatat gtttagctag ggcgcgtaat gagttaccat gtgcagctac taaaacagtt
    55261 ttgttgtcta gtaatgctgg agcaatttca tcttgccaga atggcaaagc gcgttctaat
    55321 gtcactttca agttttctcc accaggaata tcgcgtttgt ctaacattgc gtaacgacgg
    55381 tcatttgctg cagaaccttc gtcagtttct tccattaatg gaggaagagt atcgtaagaa
    55441 cgacgccaga tgtgcacttg ttcgtcgccg tatttttcag ctgtttcttt tttgttcaaa
    55501 ccttgtaatt taccatagtg acgttcgttt aagcgccatg atttgatttg tggtacccaa
    55561 agttgatctg aatattctaa gatcaagtta catgttttga ttgcacgagt taaaacagaa
    55621 gtgtaagcaa catcaaattc gattccagct tctttgattt tgcgtccgcc ttcttttgct
    55681 tcttcgattc cttctggtgc tagatttaca tcagcccaac cagtaaattg gttcaatgca
    55741 ttccattcac taagaccgtg acgagaaaaa actaattttg gcatgtttag ctctcctttt
    55801 aagataggtt tatatttctg aaattttcag ttctgtttta ttgtacaatt ttttggaaga
    55861 gatttccatc gaaaaattag ataagattct atgagaaaac aagtagagaa tataaaaaaa
    55921 cgaacagtgc ccacgcaact gttcgttttt aggtattaga tgcataattg ctcgatttct
    55981 ttttcaggta aattgatata tttataacat gttccaactg aaacaccgca ttttgaagaa
    56041 ataaattgga tcgtttcttt tttttcataa tagagacgtc ggattttttt gatcgttttt
    56101 gcatcgattt ttggtcttcc gccgattttt ccttttttac gagcttcatc taatccaact
    56161 aatgtacgtt ctttgatcaa tgcgcactcc atgtttgcta ggttttccat taattggaaa
    56221 tagatttttc ccatttcgcg cgtatcgatt ccttcatcaa gactaatcaa gtgcagcccg
    56281 cgatctttga ataattgcgt gagttctgtt aattgccgcg tagatttgcc taatcgatga
    56341 agttggcaaa tgactagtgc atcgccttgt tgcatgtcag cgatcactga ctccaaatga
    56401 acagcagtag aatcattttg ttccacttca tatgtctcat ggaaaatttc ttgacaacca
    56461 aagtcagata attgatcaac ctgagttgtt aaatcaacgt catttatggt tgtgcgtgca
    56521 taaccaataa ttttcatggt gattcctttc tagcagtgat tttctaactg gatataacag
    56581 taaaaatgta gcataaagta cgtcaaaaag atagtttttt cgcaaatttt ccgctgtctt
    56641 tttttattgg atgatcaagc agtttttctt tatttacgac ggattgcgtc aagaatctca
    56701 acgccttttt ctgagccgac aactacttta gaaaccatgt ttaggaaaag cccatgttct
    56761 actaccccaa caagttcatc taaataggta gctaagcttt ctggctgttc gatgacttct
    56821 agatgcagat caatgatgta atgaccacca tctgtggtta agacttctcc gttttcgtct
    56881 gtacgcaaga cgggttggaa tcttttttct tcaaaaatat gaagtagctg ttgggagcca
    56941 taagggatca cttcaactgg gagtgggaac ttcccaagtt tatcgactaa tttagaagaa
    57001 tcaacgatcc aaatcgtttc ttttgagtac gtagcgacta ttttctcgaa aagcaaagca
    57061 gcgccgccgc ctttgatccc ttgaaaatca gcactgattt catcagcacc gtctattgtc
    57121 aaatcgacta cggggacttc atcaatactc tttaacggta ttcctaattt ttccgcttgt
    57181 tcttttgtcg catttgaagt agtaactcct gtgatacgta aaccttcttc acgcatacgg
    57241 cgaccaatct cttctaccat ataataggcg gttgaaccgg tcccgagacc tacgatcatc
    57301 ccgtccttta cgaatttcgc ggcttcaatg ccagctaatt gttttaaatt catctttgcg
    57361 tccacctttt tttagtttga gcatcgtgct ttcctcacta ttttagcgtg atttacggta
    57421 aaaagatacc attctttttt attccaataa agctttttca aaaaaaattg catctcttgt
    57481 tgacatgttt tttcattcgt gatattctaa caaaggtgct ttatctacag gtttctgtga
    57541 agaaacgtgc tgactgttta tcaaagcata tttgataagg atgcagacat tgcatttatt
    57601 ttttatcaca aaaaaagaac cacctggatg tgtggaacta gacgcaaatc gtgggaagga
    57661 ggaaacaagg aatgcctaca attaaccaat tagtacgtaa acctcgtaaa tcaaaggtgg
    57721 aaaaatctaa ttcacctgct ttgaacaaag gatataacag ctttaagaaa actcaaacta
    57781 acgtaaactc acctcaaaaa cgtggggtag caactcgtgt gggtacaatg acacctaaaa
    57841 aaccgaactc agctttacgt aaatatgcac gtgttcgttt gtcaaactta attgaagtta
    57901 ctgcttatat cccagggatt ggtcataact tacaagaaca cagtgttgta ttattacgcg
    57961 gtggtcgtgt aagagactta ccaggggtac gttaccatat cgttcgcggt gctttagata
    58021 ctgctggtgt taacgaccgt aaacaaagcc gttctaaata cggaactaaa aaacctaaag
    58081 cttaatttta attattaaac aaatgaaact tgcttctact atatagaagt aattactatg
    58141 aacaatattt cggaaggagg agttacggat gccacgtaaa ggtcctgttg caaaacgtga
    58201 tgttttacca gatcctattt ataactcaaa attagtaact cgcttgatca accgtgtaat
    58261 ggttgacgga aaacgcggga tttcagctaa cattatctat aatgcctttg acttgatcaa
    58321 agaatctaca ggtaacgatc cattagaagt gtttgaacaa gcaatgaaaa acgttcaacc
    58381 tgttcttgaa gttaaagcac gtcgtgttgg gggttcaaac taccaagtac cagttgaagt
    58441 tcgccctgaa cgccgcgtta ctctaggtct tcgttgggta gtaaactacg ctcgcttgcg
    58501 cggtgaacac acaatggaag aacgcttagc aaaagaaatc atggatgctg caaacaacac
    58561 aggtgcttct gttaaaaaac gtgaagacac tcataaaatg gctgatgcca accgtgcgtt
    58621 tgctcactat cgctggtaag attctctctg tttggtcggc ttttataagt cgacctcaga
    58681 tagtgtcctt atttttagca actagatagt aaagagagga gaaacaagga gaatgaccag
    58741 agaattttct ttacaaaaca ctcgtaatat cggtatcatg gcccacatcg atgctggtaa
    58801 aacgactgct actgaacgta tcttgtacta tactggtaaa atccataaaa ttggtgaaac
    58861 tcatgaagga gcttcacaaa tggactggat ggaacaagaa caagaacgtg gtattacgat
    58921 cacttctgct gctactacag cacaatggaa gggtcaccga atcaacatca tcgacacacc
    58981 aggacacgta gacttcacag ttgaagttga acgttcattg cgtgtacttg atggtgctgt
    59041 aactgttctt gatgcacaat caggtgttga acctcaaact gaaacagttt ggcgtcaagc
    59101 aacaacttat ggtgtacctc gtatcgtatt tgcaaacaaa atggacaaga tcggtgctga
    59161 cttcttatat tcagtatcaa ctttacatga ccgtttgcaa gcaaacgctc acccaatcca
    59221 attaccaatt ggtgcggaag acgacttcac aggaattatc gaccttgtaa caatgaaggc
    59281 tgaaatgtat acaaacgatc ttggaacaga gatcgaagaa acagaaattc ctgaagaata
    59341 ccgcgaatta gctgaagaat ggcgcgaaaa attggttgaa gctgtagctg aaactgatga
    59401 agaacttact ttgaaatact tagaaggtga agaaatcact gaagctgagt taaaagaagg
    59461 tatccgccgc gcaacagtta acgtagaatt ctacccagta ctatgtggtt cagcatttaa
    59521 gaacaaaggg gttcaattac tattggacgc tgttcttgat tacttgccat caccgttaga
    59581 tatcccagct atcaaaggga ttgaccctaa aactgatgaa gaagtagaac gtcctgcaga
    59641 cgacagcgct cctttctcag ctttagcatt taaagttatg actgacccat tcgtaggtcg
    59701 tttaactttc ttccgtgtgt actctggtgt tttacaatca ggttcatacg tacaaaacgc
    59761 aacaaaaggc aaacgtgaac gtgttggtcg tatcctacag atgcatgcca actctcgttc
    59821 agaaatctct gaagtttacg ctggagatat cgctgctgct gttggtttga aagacacaac
    59881 tactggggac acactatgtg atgaaaagag tctagttatt cttgaatcca tggaattccc
    59941 agaaccagtt atccaagtcg ctattgaacc taaatcaaaa gctgaccaag ataaaatggg
    60001 tgtggcttta caaaaactat ctgaagaaga tcctactttc cgtgctgaaa caaacgtgga
    60061 aacaggcgaa acaatcatcg ctggtatggg tgaacttcac ttagatatca tcgttgaccg
    60121 tatgcgtcgt gaattcaaag ttgaagctaa cgtaggtgcg cctcaagtat cttaccgtga
    60181 aactttccgt gctggcactc aagccgaagg taagtttgta cgtcagtctg gtggtaaagg
    60241 tcaatacggt cacgtatgga ttgaatttac accaaatgaa gaaggtgctg gtttcgaatt
    60301 tgaaaatgcg atcgtcggtg gggttgttcc tcgtgaatac attccagctg ttgaaactgg
    60361 tttgaaagat gctatggaaa atggtgtctt agctggctat ccattagtag atatcaaagc
    60421 taaactttac gatggttcat accatgatgt cgattcaaat gaaactgcct tccgtgttgc
    60481 tgcttctatg gcattacgtg cagcagctaa gaaagctaat ccagtaatcc ttgaaccaat
    60541 catggctgta gaagttgtta tccctgaaga ttatttaggt gatgttatgg gtcacgtaac
    60601 tgctcgtcgt ggacgtgttg aaggtatgga agctcgcgct ggcggccaac aagttgttcg
    60661 tgcgatggtt ccacttgctg aaatgtttgg ttatgctaca actcttcgtt ctgctactca
    60721 aggtcgtggt acatttacaa tgacatttga ccactacgaa gatgtaccaa aatcagtaca
    60781 agaagaaatc attaagaaaa acggcggaaa agctgaataa tttcttgaaa ccggatagtc
    60841 ctgcaaagag attatccaaa gagactatat ttttaatata gtttcgtgta aaataattat
    60901 cgatgatatg ggcatgtggt gtaccttgtg tacatcatat acctatcaga caaataaaaa
    60961 atatattgta tttttaggag gacattttaa aatggcaaaa gaaaaatttg accgttctaa
    61021 accacacgtt aacattggta ctatcggaca cgttgaccat ggtaaaacta cattaacagc
    61081 tgcaatcaca actgtactat ctaagaaaaa cggcggccaa gctatggctt acgatcaaat
    61141 cgatggtgct ccagaagaac gcgaacgcgg aatcacaatc tcaacagctc acgttgagta
    61201 tgaaacagat acacgccact acgctcacgt tgactgccca ggacacgcgg attacgttaa
    61261 aaacatgatc actggtgctg ctcaaatgga cggagctatc ttggtagttt ctgctgctga
    61321 cggcccaatg cctcaaactc gtgaacacat cctattgtct cgtcaagttg gtgttcctta
    61381 catcgttgta ttcttgaaca aagtagacat ggttgatgac gaagaattac tagaattagt
    61441 tgaaatggaa gttcgtgacc tattaacaga atacgaattc cctggtgacg atgttcctgt
    61501 agttgctgga tcagctttga aagctctaga aggcgacgct tcatacgaag aaaaaattct
    61561 tgaattaatg gctgcagttg acgaatacat cccaactcca gaacgtgaca acgacaaacc
    61621 attcatgatg ccagttgaag acgtgttctc aattactgga cgtggtactg ttgctacagg
    61681 tcgtgttgaa cgtggacaag ttcgcgttgg tgacgaagtt gaagttgttg gtattgctga
    61741 agaaacttca aaaacaacag ttactggtgt tgaaatgttc cgtaaattgt tagactacgc
    61801 tgaagctgga gacaacattg gtgctttact acgtggtgtt gcacgtgaag acatccaacg
    61861 tggacaagtt ttagctaaac caggtacaat cacacctcat acaaaattct ctgcagaagt
    61921 atacgtgttg acaaaagaag aaggtggacg tcatactcca ttcttcacta actaccgtcc
    61981 acaattctac ttccgtacaa ctgacgtaac aggtgttgtt gaattaccag aaggaactga
    62041 aatggttatg cctggcgaca acgtaacaat ggaagttgaa ttaatccacc caatcgctat
    62101 cgaaaacggt actaaattct caatccgtga aggcggacgt acagttggtg ccggtgtcgt
    62161 tacagaaatc aaagcttaat tcacttaagt tttaaagtaa agcaacattt attcggacgt
    62221 aatcgctcgg aacgaaggtc ttaggatctt tgttccgagc ttttttattt att
//
LOCUS       DACDIH010000009        61729 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDIH010000009 DACDIH010000000
VERSION     DACDIH010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.009"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(59..187)
                     /locus_tag="IV281_000568"
     CDS             complement(59..187)
                     /locus_tag="IV281_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8779609.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IV281_000569"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IV281_000569"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8779610.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="IV281_000570"
     CDS             complement(1674..2729)
                     /locus_tag="IV281_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8779611.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IV281_000571"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IV281_000571"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP8779612.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IV281_000572"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IV281_000572"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP8779613.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IV281_000573"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IV281_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP8779614.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IV281_000574"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IV281_000574"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP8779615.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IV281_000575"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IV281_000575"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP8779616.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMISYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="IV281_000576"
     CDS             10491..12050
                     /locus_tag="IV281_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP8779617.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="IV281_000577"
     CDS             complement(12161..14092)
                     /locus_tag="IV281_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8779618.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IV281_000578"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IV281_000578"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP8779619.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="IV281_000579"
     CDS             complement(15021..15773)
                     /locus_tag="IV281_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8779620.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="IV281_000580"
     CDS             complement(15932..16141)
                     /locus_tag="IV281_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779621.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="IV281_000581"
     CDS             complement(16152..17480)
                     /locus_tag="IV281_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779622.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(17845..18432)
                     /locus_tag="IV281_000582"
     CDS             complement(17845..18432)
                     /locus_tag="IV281_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP8779623.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="IV281_000583"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="IV281_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP8779624.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /locus_tag="IV281_000584"
     CDS             complement(20012..21301)
                     /locus_tag="IV281_000584"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP8779625.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="IV281_000585"
     CDS             complement(21455..22387)
                     /locus_tag="IV281_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779626.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="IV281_000586"
     CDS             complement(22544..23203)
                     /locus_tag="IV281_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP8779627.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="IV281_000587"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="IV281_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP8779628.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGASGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="IV281_000588"
     CDS             complement(24244..24534)
                     /locus_tag="IV281_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779629.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24944..26749
                     /gene="glmS"
                     /locus_tag="IV281_000589"
     CDS             24944..26749
                     /gene="glmS"
                     /locus_tag="IV281_000589"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP8779630.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26821..28176)
                     /locus_tag="IV281_000590"
     CDS             complement(26821..28176)
                     /locus_tag="IV281_000590"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP8779631.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28200..29369)
                     /locus_tag="IV281_000591"
     CDS             complement(28200..29369)
                     /locus_tag="IV281_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779632.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29366..30253)
                     /locus_tag="IV281_000592"
     CDS             complement(29366..30253)
                     /locus_tag="IV281_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP8779633.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="IV281_000593"
     CDS             complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="IV281_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP8779634.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            34320..34841
                     /locus_tag="IV281_000594"
     CDS             34320..34841
                     /locus_tag="IV281_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8779635.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35540..36105)
                     /locus_tag="IV281_000595"
                     /pseudo
     CDS             complement(35540..36105)
                     /locus_tag="IV281_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36284..36940)
                     /locus_tag="IV281_000596"
     CDS             complement(36284..36940)
                     /locus_tag="IV281_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779636.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="IV281_000597"
     CDS             complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="IV281_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP8779637.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(38477..39475)
                     /locus_tag="IV281_000598"
     CDS             complement(38477..39475)
                     /locus_tag="IV281_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP8779638.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="IV281_000599"
     CDS             complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="IV281_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP8779639.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(40491..41222)
                     /locus_tag="IV281_000600"
     CDS             complement(40491..41222)
                     /locus_tag="IV281_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP8779640.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(41226..42491)
                     /locus_tag="IV281_000601"
     CDS             complement(41226..42491)
                     /locus_tag="IV281_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP8779641.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="IV281_000602"
     CDS             complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="IV281_000602"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP8779642.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="IV281_000603"
     CDS             complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="IV281_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP8779643.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            47833..49335
                     /locus_tag="IV281_000604"
     CDS             47833..49335
                     /locus_tag="IV281_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8779644.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            49337..50074
                     /locus_tag="IV281_000605"
     CDS             49337..50074
                     /locus_tag="IV281_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8779645.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="IV281_000606"
     CDS             complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="IV281_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP8779646.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="IV281_000607"
     CDS             complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="IV281_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP8779647.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="IV281_000608"
     CDS             complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="IV281_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP8779648.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="IV281_000609"
     CDS             complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="IV281_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP8779649.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(55574..56740)
                     /locus_tag="IV281_000610"
     CDS             complement(55574..56740)
                     /locus_tag="IV281_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP8779650.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGNRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(56756..56983)
                     /locus_tag="IV281_000611"
     CDS             complement(56756..56983)
                     /locus_tag="IV281_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP8779651.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            complement(57370..57626)
                     /locus_tag="IV281_000612"
                     /pseudo
     CDS             complement(57370..57626)
                     /locus_tag="IV281_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            complement(57969..58992)
                     /locus_tag="IV281_000613"
                     /pseudo
     CDS             complement(57969..58992)
                     /locus_tag="IV281_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(59630..60304)
                     /locus_tag="IV281_000614"
                     /pseudo
     CDS             complement(59630..60304)
                     /locus_tag="IV281_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313658.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            60827..61417
                     /locus_tag="IV281_000615"
     CDS             60827..61417
                     /locus_tag="IV281_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP8779652.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
BASE COUNT        17125 a        13258 c        10687 g        20659 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgagatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    17521 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt aacacggaac
    21661 taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccta
    22621 gatgttgctt tttctgatta aagattgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagatac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagcc cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttgaagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt catcttctac
    24361 cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    24781 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    24841 ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt cctgcagctg
    24901 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    24961 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    25021 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    25081 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    25141 aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    25201 acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg aaaactttga
    25261 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    25321 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    25381 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    25441 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    25501 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    25561 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    25621 catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg agaaaggtac
    25681 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    25741 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    25801 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    25861 aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt tagccagtga
    25921 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    25981 tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg gctacccttc
    26041 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    26101 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    26161 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    26221 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    26281 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    26341 cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    26401 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    26461 gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg gtgcgcatac
    26521 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    26581 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    26641 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    26701 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    26761 ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg aaaatatctc
    26821 ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat agtaattaac
    26881 tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac cggaaggacg
    26941 aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca cttttttgat
    27001 tgccggaaca tccatcgcac cgtttttatc acttactcgg atattcacta atttttgcgg
    27061 atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt tcatgatatt
    27121 cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta agaaaatcat
    27181 gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt ctacaacgta
    27241 acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg ctttgtggaa
    27301 tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat ttttttctgc
    27361 caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc caagttcatc
    27421 caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg ctcctttttc
    27481 aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccatcat tgatatttaa
    27541 cccgtctgga tttgtgccca tggtataaaa atctgtttca agatcggcaa acaaacgatt
    27601 aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac cggaaaggtc
    27661 accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg gaaattcttc
    27721 tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag catctaataa
    27781 tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac cgaaaaattt
    27841 gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg cgcttgcttt
    27901 ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct ggaacacttc
    27961 gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac ctgagattct
    28021 tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct ggcttaggac
    28081 ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct tgttggcttc
    28141 accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac tcctttaact
    28201 taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc tgatgtttct
    28261 tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat ggttgttgtc
    28321 gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt attttcttgt
    28381 gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg tgtaatctcc
    28441 acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc aattttacga
    28501 gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc accgatcgat
    28561 gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact gaaatcatag
    28621 ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac cgacttgctt
    28681 ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat acttaatact
    28741 tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc atctgtgacc
    28801 atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt gtcacctgta
    28861 gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc agatggtgtg
    28921 aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt ctcaaccgtt
    28981 actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt ttgaattttt
    29041 agcggaacct catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac gcggaacatc
    29101 cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga aagtttgaca
    29161 gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga aatcttgatt
    29221 ggcaccccag taactgtttg attatacgct tcatttccag aaagcgtatt tggaacggac
    29281 tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa actgtaaaga
    29341 atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc ataccgtcta
    29401 ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatc agttctctac
    29461 gcaaaattgc caaatattct tgctgggata agccagccat cagttcatta tctaatgtaa
    29521 tgctgacatc cccagtttct tcagagacaa cgatggtaat cgcgtcgctg acttcgctta
    29581 taccgacagc tgcccggtgt cgagtcccga attcttttgg aatcaacata ctttctgata
    29641 gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact gcaccgtcat
    29701 gcagcggtgt attaggaata aagatattga tcagcagctc tccagtgata tctgcatcta
    29761 atgcaattcc tgtctcaata tactcatcta atccagtatg ccgttcgata gtaattagcg
    29821 caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag atcatcttct
    29881 catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca agatgttcca
    29941 gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg tacgtaatga
    30001 cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg attcggataa
    30061 caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc agcagttgga
    30121 tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc aaaaaattca
    30181 gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa agttccccga
    30241 tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag tatatcatat
    30301 gttttaattt tagaggctga tctgctcgta tttttcttga gaaaaacgca taattttcag
    30361 gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa cttcagagaa
    30421 ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt gttccatttt
    30481 tttacgtcgt tctgctctgg cagctcttct ttcttctgct tcaaggtccg tcgttttttc
    30541 ttttctagtc ttaacgggta catcgataga ttcctctggt tcaagtcgtt tcttgatttt
    30601 ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt cttggatagt
    30661 cttttcaaat aaagcttttg caagatttgg atttgcgttc tctagcgagg aaaagcgaac
    30721 ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt tttttgtagt ccaaagtcat
    30781 tgggttcttg tttttttcga ccattgcagg gttatatcga tataatgacc aatagccaga
    30841 agaaacagct tcttttgctt ctttaagcga ttctttcatt ccaccaagca aaccatgtgt
    30901 gatacaaggc gtataggcaa tgattaaaga aggtccagga tacttttctg cttcttcaaa
    30961 agcctttatc gtctgcattt gattggcacc tgaagcaatt tgcgccacat aaacatttcc
    31021 atatgtcatc gccatcattc ctagatcttt ctttgaagca tactttccac tagccgaaaa
    31081 tttagcgatt gccgaagctg gtgttgcttt tgaggtttga ccgccagtat tggaatagac
    31141 ttcgttatcc aaaacaagaa tattcacgtc tgctccgctg gcaagtacat gatcgatccc
    31201 gccaaaacca atgtcgtaag cccatccgtc tccaccaatc atccattggc tgttttttac
    31261 gaatagatcc tttctcttgt atatatcctc cagttcaggg acaattgatc gttcgctttc
    31321 gatcgcagct tgcagttttg ctgctctttg ctgcgttcct tcgctctcaa acatgtgtac
    31381 caaccagtct tccatcaatt ctcgtagttc ttctgatact tttggcaata cttctttgat
    31441 tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga agcataccat aaccaaattc
    31501 agcattatct tccaataaag aatttgacca tgcaggccct tgaccacatt cattggtcgt
    31561 ataaggagaa gcaggagaag caccgcccca aatagaagaa cagcctgtcg cattcgcgat
    31621 catcattcga tcaccgaaca tttgtgtcaa taattttaca taaggtgttt cgccacagcc
    31681 tgagcaagcg cccgagaatt caagaagcgg ttgattgaat tgtgtagaca agacagtatt
    31741 ctttcctttg actggatttg ctttttgtct taatgtcatt gcaaaagccc aattcacagc
    31801 ttgttctttc tgttcttcat aaggcttcat cactaaagct ttttcttttg cgggacaagc
    31861 ctctacacac agaccacaac ctgtacagtc ttccactgaa acttggatac ggtattttac
    31921 gccgtctgcg cctcgcattt ctctcacgat aaatccttct ggcgcttctt ccatttcctc
    31981 ttcatctgct aaaaatggac ggatggcagc atgaggacaa acaaaggcac attcattaca
    32041 catcgtacag cgatcactaa tccactcagg tacttctaaa gcgacgcccc gcttttccat
    32101 agaagccgtt cctaaaggca tttcgccagt tgtcattcca tttgctacaa gtgtaccaac
    32161 agagagttga tttccttcct gtgcattcac tggttcaagt atttcttgta tataaccagt
    32221 gttcctttta ttgatttttt caggcatctc caacgttctc caatggtctg ggatcgcgac
    32281 ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg tttttttcta cgatagccat
    32341 tgatttatgt ccatagcttt tcagggcttc ttcttttagc agcggaagca cctcatcaaa
    32401 aggaatgatg tcagctaatt taaaaaaagc cgtttccatt gccgtattga tccggcgtcc
    32461 caaaccgact tcttgggcca atctcatggc attgatcgta tagaatctaa tgttattttc
    32521 agccaagtaa cgttttaatt ttaagggcag atgcgtttct aattgttcgt cgctccaaag
    32581 agtattcaat aaaaaaatgc cacctggctt caatcctttg accaagtcat agctatgcag
    32641 gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg acaagatacg ctgaacgtat
    32701 cggcgtatct ccaaaacgaa gatgagagac agtcagaccg ccagattttt tcgaatcata
    32761 ataaaaatag ccttgcgcat atttatcggt gtgatctcca ataatcttga tagctgattt
    32821 atttgctcca actgttccgt cagatccaaa tccccaaaac ttggcctgga atgtttgcgg
    32881 ttctgtcaaa tccagacttt cctttggttc caaagattga taagtcacat catccacgat
    32941 ccctatggta aaccgttttt tgcgtatgct tgggtctttt ttcaattcat caaaaactgc
    33001 tgagatttgg tcgggagtca catctttaga ccccaatccg tagcgaccac caataacagc
    33061 cgggcgaagt tcagaatcat ataacgcact ttgaacgtct aataagagag gttctccacc
    33121 tgcccctggt tctttacttc gatcaagaac agcaattgtt tttactgtat gaggcaactt
    33181 ttgcacgaat gtttccactg gaaaaggacg ataaagatgg atattcaaaa aacctgtttt
    33241 ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa ctgatcccat
    33301 agaaacaatg acttcttccg catcgtctgc tccgtaataa gtcaccaagt cataagaagt
    33361 tcctcgtaat cggttgatct ctttcatata tttttgaacg atttcaggta atttatcata
    33421 ataggggttg atcgtttccc gttgttgaaa atggatgtct ggattctggt tcgtaccact
    33481 aacagtcgga tgattgggat tcatccctcg tttacgaaat gaagctaatt tctcctgatt
    33541 gatcagagga gctaattctt catattccaa aacttcgatt ttttgaatct cgtgactcgt
    33601 acggaatcca tcaaagaaat tcaaaaaagg tacttctcct tcaatagaag caagatgggc
    33661 aacaggtgct aaatccatca cttcttgtac actgctttca gctaacatag caaatcctgt
    33721 ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat ttgtcgtcac
    33781 tgcccgacta gcaacatgga aaacacttgg cagtagttcc ccagcaatct tgtacatatt
    33841 cggtatcatc aaaagcagcc cttgagaagc ggtatatgta gttgtcagtg caccagtttt
    33901 caatgatcca tggaccacac cagctgctcc tgcttcggat tgcatttcta ctacttttac
    33961 tggttgaccg aatacatttt cttttcctgc tgctgaccat tgatctacta attcagccat
    34021 ggtcgaactg ggtgttattg gatatattgc agcaatctcg gtaaatgcat aagaaatata
    34081 tgcggctgcc gtattgccat ccattgtttt catttttcgc atgattcatc atccttgttc
    34141 atattctctc ttattatctg ttccattata acgtaaaaca ttccattttt tcttttattt
    34201 ataaaaatat ttcacatatg tctatttgaa ttggtcatct aatttttaga caaacagtct
    34261 aaatcgttgc ctttttttgt cagacatgta taaactataa gcgaaagagg tgttttaaca
    34321 tggatatatc agtgattgat gcaacgaaaa caaatacaca aaaaggaatc ctttatggtt
    34381 catcgaatgc tcctaaaaaa atggtcgaat tcatcaacct atcttgtccg tattgccgac
    34441 aatggtttga agaatcttat gagctattag aagaagcagt ccaaagcggg caattgcagc
    34501 gagtgatcaa attgttcgat aaagaaaaag aaagcttgca gcgaggaaac gtgatgcacc
    34561 ggtatctcac aatcagcgat ggtcaaaaag cgatcaaaga gatcaaacaa atattcgata
    34621 ctcaagacga gtggaaacat ctttctctcc aagaagtagc ggattttgca gtagataaac
    34681 taaagctgac agaacaaaaa gacgaacagc tgagccaagc tgtcatcaac gaagctgagc
    34741 aagcacatat tcgatttgtt ccgactgtca tcttgggtaa agaaatcttt gatgaatcta
    34801 tttcaataga ggaattaaaa gaattgatcc aaacaaaata aaaaaataag gagcgtagga
    34861 caaaagtaaa gaacactttt gtctcacgct ctaaacacct ataaacggcg gaagcagaag
    34921 caactccttc ttatttcttg gagttaaacg cttctgtccc agcctcctta ttccgaataa
    34981 atggtgtcca aaagcttgca tctgccagat tatctcattt cttatgacaa gctgagcaaa
    35041 ttatctccac caaataattt attcatattt ttgacttgcg catactaact aattcttaaa
    35101 ttttcataca aaatttctca aattccctta acgagtaata tttatgtacg gtgattcaca
    35161 ctagtatccc aatcactttt taagttatcc cgatttcaat tctacttcaa aaatagttgc
    35221 ttttatgtat gctgttagtt gattagatgg tcgtttgttg agctaggtcg tttattttaa
    35281 acaagttgtt tctttagctt atacctatct cgtctaccaa acgcaagatg gcaaacacaa
    35341 atgcatacgc atctgtattt gccactttga ttcgtataaa gctaacaact cctaactcct
    35401 ctcatttaat agttgctctc ttttttattc agatacattt ttgctgatca cagcctagta
    35461 tttcaatggt tttgaacact aaaccaaatt gttccattac aacattcact ggctgatgaa
    35521 atggattaat agatttcgat tattcaaagc gattttctat tggtgaattg tgttcccaac
    35581 taacaagacg gattccaaag tcattatata actcttcaag agttaaccct ttcctgttcc
    35641 caatagatgt atctctccca cgctcgatat aaaatatgga gtttcgaatg ccaagacctg
    35701 gcaaatcttt ccttgttaca gttacactgc tactaacttc cccatataaa atccagctcc
    35761 tccaaaatga taatatattt ttcgaccatc atctaactca tgtaacaata attgttgtgc
    35821 cctcttgtca ctcgccaaat gcaaacgcca cgtgcgatac catgccatgc tacctgcatc
    35881 tgcagaaaat ccatcaaata atgcaacaat tgtatcttct atcacaatcc tttccccatc
    35941 tacttcatat tctaatctaa aatcaaactc ccctcgggta atcgcaggtc ttggtggctt
    36001 aggacccaat attattctac ttaaaaccgc attcgttccc agatgaaaca cagctataac
    36061 aacaataatg attaatattt tgtttctatt tgataaattt ctcattattc gcaaatcacc
    36121 tctacattct cacatactag ttcgtaatac tcctttaata tcaaaatatc tactgatcgt
    36181 aataatattc gctatgaatt cctactactc gtataagaaa ggccaaatca tcagctttta
    36241 cttgctgatg atttggcttg tttctattta tcgtaattgt tttctaatca ccaaaattgt
    36301 taacaatcgg ttcagatatt tcccaattca ctagccttat attatattga tttaacaatt
    36361 cttcttcatt tattgtattt tgatcaagcg gaccaaattg ttctaataat tctttgtcat
    36421 gtaaaatatc ccttctagta atgcctcttt ccctttcaat gaaagaagca ctaggaaaag
    36481 tatgattata accattcaac ctatctccca tgtaataatt agcaggtcct ggaaaataaa
    36541 atatttgctt attatcacta acttctaaca gcaacactaa atcttctccg ctggctaaac
    36601 gacttgtcca tctacgatat ctccctatgc cttcgttcat tccaaatcca tcaaattcaa
    36661 ctattaacgt gtcttctatc acatgtaatt cttcattcaa ttcatactca agtctaaaag
    36721 gaaattctcc atatgtaatc tctggcaatg gtggtgctgg agttctccaa ataaatacaa
    36781 taactgtgaa tagaaaaaat ccaacgatcg atataaatgt tatgagacct gctcgaagta
    36841 aaacccgata taataaaaat gctgtatttc gctttgctat atctagaaaa ataaaaagta
    36901 ctgcaatcat aatgatagta aaagttgtaa aaaaacgcat ttttgccctc cctttataaa
    36961 catcgattct caaattctaa accaaatctt ggtagttcaa gttcaggtat aacgtattcc
    37021 aaccatacat ctcaattact atttatgaac ttatgattat cactgaataa atcactccat
    37081 ccccttgtta aaacttctac attaattaca cctggttgtt ttcgactagc taatccaatg
    37141 aacttatcaa ccatccttat ccaccaacca cgagaatctt gaccagaagt aagaagagca
    37201 gttctcaagg atgatcgagc agttccttcc agttttttaa ttaatcaggc cagttataga
    37261 taaatacttg atcttatcta tatcaggact tctatattaa gcaaaaattt atgtacaaaa
    37321 ctaaaatctt cattcagttg tctccccgct ttttatacga ttgtattaaa aacaatagaa
    37381 gcctttccaa attatgttac tccagtaaag atggaattat agtttgacgg aagaaaagca
    37441 agtataactt ttaaaaaaaa ctgatacatt cgcatctcct gcttcatgta tcagtttttt
    37501 ctttggatta aactgcttct tcacgcagtt tttccgcaaa ttcatttatt ttgcgaattc
    37561 ggtgattaat tcccgatttt gatatcgcac ctgagggaat catttcccct aattctttca
    37621 agctgacctc tggatgttga agccgaagtt ctgcgatttc ctgaagtttt tcaggaaggg
    37681 catgtagacc gacctttgct tcgatcaatt cgatgttttc gatttgttta gaagccgcat
    37741 caatcgtctt gttcagattt gctgtctcac aattgactag acgattgacc gaatttctca
    37801 tatcccgtac gattcgaaca tcttcaaatc ggagcattga gttggttgca ccaatcaatg
    37861 ttaagaaatc agcaattttt tctgctcctt ttaaataaga aatatagcca ttgcgccgtt
    37921 caagcgttcg agcatgtaga ccgtaataat ttaacatatc gcaaatgtct tggttatgct
    37981 gttcataaat agaaaatatt tccaaatgat aacggctcgt ttccggatta ttgactgaac
    38041 ctgaggccat aaaagcacca cgtaagtaag aacgcatttt ttgcgaattt cccataatct
    38101 cgtccgaaac gtgtccattg aacatcatac catccatgat atcaagatcc atcaacacat
    38161 cttttgtgcc ttgcttcaac cggacgatat agacattgtt cttttttaat ttcatttttt
    38221 tacgaacgag tagctcactt cgtacctgat aatgatcttt caaaagagaa tagatacgtc
    38281 tggcaattgc agcattttct gtttggacgt tcaaaataaa ctgctggttc acaataccta
    38341 aagagccgtt cattcgcagc aaagcagcta attccgcttt cgcatgttct cgatgaactt
    38401 ccaaaccagt tagttctttc ttcacatctg cagcaaatga catcctattc tcctcctttc
    38461 tccaacaata aattgcttaa tattttgaac cgaaaactaa acggaacaat tcttctacta
    38521 ctttgtctcc atcgtggaag acaccaccat ctttcaattc caaaaagtcg gtggaaatca
    38581 ctcgacatcc ttcatttctc aatccgttga agtcgtgtgc gacttgtact agatactcat
    38641 catagatctc tgggtccata taatcttcag gcactttttc cgtattgacc aatactgtat
    38701 cgataaaagg ctgattcaaa tgcttgtgta aaacacgtat atgatcggca tctgtgaaat
    38761 gttccgtttc ccctttttgg gtcatgatat tacagatata gacggtttca gcagttgtct
    38821 tgagcattgc ttcgccgatt tctggtatca ctaaatttgg aaggatactg gtgaataaac
    38881 ttccgggtcc caaaacgacc atgtcggcgt ccataattgc tgaaacgact tttcgagccg
    38941 ctttggcttc acttttatca tttgtgttgc agacatacac acggtcaatg gttttacggt
    39001 cgatcgcgat tttagattct cctactgcag tcgttccgtc tttaaagaca gcatgcaagg
    39061 tcaaaggagt ttctgaagaa ggataaacat gtccatctac atgcatcatt tttgctaata
    39121 attgaatcgc ctcataagtg cttccccgca tctcggaaac tgcggcaatg atcagattac
    39181 cgatcgtatg attggctaaa aactgatctt cctttttgaa acggtattgg aaaatatctt
    39241 catagaattg tggcatatcc gataacgcca ctaagacatt tctcaagtca ccaggaggag
    39301 ccatagcgat cgattctctg attgcaccgc tgcttccccc atcatctgca acagtcacta
    39361 ctgctgtgat atctgctcct tgatttcgca aacttttcag gataactggc agaccagtgc
    39421 caccgccgac cactacgatc tttggttttc ttatccggta ggttttcatt ttcacgaccg
    39481 gttcaccgtc tctttccttt tatccttgtc ccgatgtgtc acattgactt tatagttttt
    39541 ccctaaagca tttgccaaac gttcagtcaa tgccacagaa cggtgttgac cgcctgtaca
    39601 gccgattgca actgtcaagc tgctttttcc ttctttaatg tatccaggca atacagtttc
    39661 cagaagatcc aaaaatttat ggtagaatga ttgcgtttca tcaaaggaca tcacataatc
    39721 atagactggc tgatcttttc cggtcaatgg tcttagttcc gggatataat gagggttggg
    39781 caaaaaacgc acatccatca caatatccga atcgatcggc aacccgtatt taaaaccaaa
    39841 tgatacaaat tcgatccgaa atccttggtc tccctttgat ttgaatgact cattgatttt
    39901 ttcacgtaat tgtcgtggtg tcaatgtcgt cgtatcaatg atcacggaag cttgggcttt
    39961 caagtcgtct agtatcgccc gttctttacg tatcccttca gtgatcaaac cgtccatcgc
    40021 cattggatgt gctcgtcttg tttctttgta gcgagccact agttcttcgt ctgaagcatc
    40081 taaaaataag atagaagtgt cgatgaagtt cgtattttcg atttcgatca gcatgttttg
    40141 gatttcttca aagaaggaac gagaacgtaa atccactcct aaagccattt ttgtgacttt
    40201 gcctgattct ttgattaatt cccagaattt tggaatcaat cgaggcggca tattatcgat
    40261 acaaaaatag cccatatctt caaaactttg gatagctact gttttccctg caccgctcat
    40321 acctgttatg atgactaatt gcaaattatc tgtcataatt acgaactcct ctcacattgt
    40381 gcatagtaaa attatatcat tccgggcgta tcattaccag ttttcttttt cttttacaaa
    40441 aagatttgga ccaaatatct tttaggatat ttgccccaaa tccaattttt ttacttttcc
    40501 gatgctgtat ccaatgcctt gcttctttca gctagcgctc gttcaatcgt ccgatctgct
    40561 aaaatagatt gggcatcgat cactggataa tggttatctt cagcaaattc atgcatcaat
    40621 gacaattctg tgcaacctaa gataactttt tcacagttta ggcgctctac cgcttcttct
    40681 aagatttcgt aatatagctc ttgattcaaa taatccgatt ccttgatctc atgatagatc
    40741 agataattga ttttttcttg aagaacagta tcaggaatca ctgtttcgaa tccaagattt
    40801 ttgacttcgc gttcatagat tccagctttc atacttcctt ctgttcctag aatcgctact
    40861 ctccctgtcg tatgctgacg gacaagttca tttgctgctt ctcttggcat atgaaggata
    40921 ggaatatccg ttgccgcttg taattcttca aaaaagtagt gtgcggtgtt acaagtcaac
    40981 acaatgaagt tcggctgcaa taaattttgt ttctcaatat catccagcag aaacggcatt
    41041 ggattttctt ctgatcgatc taaaatataa gctgtacgat caggaactgt tgcatgattg
    41101 aataagacat aattcaaata ttcttgatct tttgtagctt tcgttcggtg gttgatcaag
    41161 cgaacaaaac tttctgtcgc catcgtgccc attccgccta aaatactgaa aaaattctcc
    41221 atgaactagc cttctttcac atgaaaatac tttttgaatc ttggaatata gttatggaac
    41281 atatatttca tcaacaacca gcgcttgatt gaccggtctt tttcataaaa cacagtagta
    41341 ccgtaacgat tttctttgat catttgctca gctagttttt tgtcttcatt ttcccgagca
    41401 tattctaaaa agattttttt gggcacaccc atccataatc ttgctttatc tgattgattc
    41461 gttccatagg tcgtttcaac aaagggtttg ttaaacactc gatcttctgt gacgaaacga
    41521 gccagattca agccgttcaa tgtcacaaag aaactgctac gaccttgtct caaattgatt
    41581 tcaaataatt tatattctcc atctctagga tcatacttca tatcgaaatt ggcaaagcct
    41641 gtatattcga ttttttccaa aaatgcctta atggtttgat agattttttc gttataatct
    41701 ggcatgatca caacgtaatt accaatcgat gctggtgtag gatcttctaa cagtggatga
    41761 ccaagacaca tcatccgaac ttgatgatct tcatctacat acgcatttag cacacgcata
    41821 ttactatcat cacccgggat aaaatcttgt acaatcattt cacttttata accggcttcg
    41881 tatatccggc ctaaaattaa atcgaactct tctctagtct ctaaaataaa agcttttttt
    41941 cttccttcaa attggacaga aagatactcc acactgtttg caggtttcaa agcaactggg
    42001 aattcaaaag gcaattcctg ttccaaatgt ccatttacta acatttcttc tctgacgatc
    42061 aatgttttag gatacggaag atcatattct tcacaaactt cataaaagct tactttattg
    42121 atcagtcttt caaataaaga ataatcaata tatggacaga taaaatattc tgacagctct
    42181 tgtttatgtt gggagatcaa ttctgcatat ccatcaccac aagcaatcaa aagatatttt
    42241 ttactcttat cagaatatct ttcttttgcc aaacgaagca tcgtctcgat aaacacaggg
    42301 tctttatcaa atcctggaat aacttctaca ttgacaattt tactgtagcg ggtaggggct
    42361 aactgatccg aagcatatgc ttggcagatt ttgccgtatg cttcattaaa agaacgtgcc
    42421 ataccataaa cgttcatgtc gcttcctaat aatataggga taaattcttc attttcaata
    42481 ctgttcatca ttatctctca cttactcata atctcttgtt tttcttttcc aactacacaa
    42541 ggaatatcat accataaaga aaaaagcaat caaaggaaac tcctccgatt gctcaattct
    42601 tttttgaacg gattgaactt ataggaagtt tttgtctata ccaatcagtg tagaaagaac
    42661 taccatgtaa tgattctaga acactgatat taataaaaca agcaaaaatt cattttttta
    42721 tgttttttta actatctcag ctatctatcc tctttatacc attatttttt tcaaatagtg
    42781 acctgtataa ctttctttta cgttaatgat ttcttctggt gttccagtcg caaggatcgt
    42841 tcctccacct tctccacctt ctggtcctaa atcaatgata taatctgctg ttttgattac
    42901 atctagattg tgttcaatca ctaaaactgt gttaccagca tctactaatc tttgtaatac
    42961 atgcaacaag cgggcgatgt catcgctatg aagtcctgtc gttggttcat ctagtatata
    43021 gaaattcttt ccattagaga ttttgtgcaa ttcactggca agtttcatcc gttgtgcctc
    43081 accaccggac aatgtcgttg ctggttgccc catcgtcaca taacctaagc caacatcaac
    43141 aatcgtttgc agtttgcgat gaatctttgg aatgtgcttg aagaattcta cagcatcttc
    43201 tactgtcatt tccaaaatat cagcaatgct ttttcctttg taatgcactt ctaatgtttc
    43261 agagttatat cgtttgccat gacatacttc acaaggaaca tagacatcag gcaaaaagtg
    43321 catttcgatc ttaataattc catccccgcg acaagcttca caacgaccgc cttttacgtt
    43381 gaaactaaaa cgccctttct tataaccccg catttttgcc tcgttcgttt gagcaaataa
    43441 cccacggata tcatcaaata cacttgtata agtcgctgga ttacttctcg gcgtccggcc
    43501 gattgggctt tgatcgatat cgatgatctt ttcgatactt tcgtagccgg aaattgtctt
    43561 gaatttacct ggcttagcag aattcttatt taatttttgc gctaacgatt tctttaagat
    43621 actattgacc aatgtactct ttccagaacc agacacacct gtgactgcta caaattctcc
    43681 taatggaaat tcaacactaa tatttttcag attgttttct gaagcaccag tgatcttgat
    43741 tgctttccca ttacctttac ggcgttcttt tggtacaggg attgattttt tccctgaaag
    43801 atatttccct gtcaaagaat tttcatcatt agctacttct tcaggagtgc ctgcagccac
    43861 gatttgtccg ccttgttctc cggccccagg tccgacgtca atcagataat ctgctgccca
    43921 catcgtatct tcatcatgtt cgactacaac gagggtgttg ccaagatcac gcattttctt
    43981 taatgagcca atcagtcgat cgttgtcgcg ttgatgcaat ccgatcgatg gttcatctaa
    44041 aatatacaaa actccagaaa gattcgatcc aatttgtgta gccaatctga ttcgctgcgc
    44101 ttctccccca gataatgttc cggaagcgcg actcaaagtt agatagtcta gtcccacatt
    44161 ttccaagaat gaaagtctgt catttacttc ttttaaaatc ggtttggcaa ttactttctc
    44221 ttgttcagat aacgttaagt ttgagaagaa atctactgct ttttgaatag acaactcatt
    44281 tgtttgcccg atattcgttc cattgatttg aacagataga gcttggggat tcaatcgata
    44341 acctttacat gtctgacaag ttaattctgt catataaaga cgcatttgct cacgcgtgaa
    44401 atcactgttc gtatcatgat aacgacgctc gatattcttc aatacccctt cgaaagtcgt
    44461 ttctacatcg cgaacgccac caaaatcatt ttcataatgg aaatggaaaa gatcatctgt
    44521 cccattcaag atgatatcct gatgctcttc cggtaattct tcgaatggtg tatctaaatc
    44581 gataccgaaa ctttcacaag cctgcgctaa catttgagga taatactgag agctgattgg
    44641 attccatgga acgatagcac cttgactaag tgtcttcgtt ctatctggaa tcactaaatc
    44701 ttgatcgact tctaatttga cacctaaacc atcacagtct ggacaagctc caaaaggcgc
    44761 attgaatgag aacagtcttg gttctagttc gccaacagta aatccgcaat aaggacaggc
    44821 atagtgttcg ctaaacaaca tttcttcttc accgatcacg tcaaccagag catagccgtc
    44881 tgccaatcgc agagccgcct ctaaagaatc aaatagacga gaacgaatcc cttctttcac
    44941 aatgatccgg tcgatgacga tcgcgatatc atgttttttg tttttctcca gctcaggcac
    45001 ttcacttaaa tcatagatct caccatcgac tcgaacgcgg acgtatccct ctttttggat
    45061 tcgttcgaat atttttttat gttgtccttt tttctttacc acgatcggtg ccaacacttg
    45121 gatctttgtg cgctcaggca aagccattac ttgatctgcc atttgatcta ctgattgact
    45181 ggtaatctcg atatgatcgt tgggacaaat cggatgtcct acacgtgcaa acaacaagcg
    45241 taaatagtca ttgatttccg tcacagtacc tactgtcgac cgaggatttt tacttgttgt
    45301 tttctgatca atagaaatcg ccggactcaa tccgtcgatg ctatctacgt ccggcttatc
    45361 catttgtcct aaaaactgac gagcataagc agaaaggctc tctacgtatc tccgctgccc
    45421 ttcagcgtac agcgtatcaa atgctaggga actttttcca gaaccggaca aacctgtaac
    45481 gaccacgaat ttatctcgtg gaatcgttac atctatattt ttcaagttat gggcacgtgc
    45541 accgtgaatc gtaattttat cgttagccat gttgtcctcc tactttgaag cttttaattc
    45601 taaaatcata tctcgcacat tagcggctgt ttcaaaatcc aatgctttcg ccgcattttt
    45661 catttctttt tctaatttca tcaataacgt atctttttct tctttcgtca tttcttcata
    45721 cgagacttgt accgcttctt ttgtatcatc ttccgattct ttcgtaatag aaatcaaatc
    45781 tcggatttct ttaatgatcg tttttggaac gatcccatgt tcttcattgt acttctgctg
    45841 aatcgtacgg cgtcttgatg tttcatccat cgctagacgc atagaatctg ttactttgtc
    45901 tgcgtacatg attactttac cttcttcatt ccgagcagct cgtccaatcg tttggactaa
    45961 ggaacgttca ctacgtaaaa agccttcttt atccgcatcc aagatagcaa caagggaaac
    46021 ttcaggtacg tctaatcctt cacgaagcaa attgattcca actaacacat caaattcacc
    46081 taatcgaaga tctcgaatga tttctgttcg ttccagcgtt ttgatatcac tatgcaaata
    46141 tttgactttg atccctaatt ctttgaaata atctgtgagg tcttctgcca tttttttcgt
    46201 caatgtcgtt acaaacaccc gctggtcttt ttcgactcgt tcatttattt cgcctactag
    46261 atcatcgatt tgtcccatga ttggacgaac ttcgatcact gggtcaagca gccctgtcgg
    46321 acggatgatt tgttggataa cggtatctgt ttgttcatgt tcatatggtc ctggcgtcgc
    46381 agagacataa atgatctgat tgacatgttt ttcaaactct tctaaccgaa gtggccgatt
    46441 gtctaaagca gatggcaaac ggaaaccgta atctactagc atctgttttc tcgctcggtc
    46501 accattatac attcctctga cttgaggcat ggttacgtgc gattcgtccg ccacgatcaa
    46561 aaaatcttca ggaaagaaat ccaatagcgt ataaggcggt tcaccttctt tacgtccgtc
    46621 catatgccga gaataatttt caattcccga cgtgtagccc atttctaaca tcatttcaat
    46681 atcgtaattg gttcgctgtt ctaaacgttg agcttccaga agtttgtttt catttcgcaa
    46741 gacagttaaa cgctgttcta attccgcttt gatattcgct acggcatgct ccatatgttc
    46801 atcattcgtc acaaagtgag tcgccgggaa gattgccacg tgttccgttt ctcctaaaac
    46861 ttcacctgtc agtgcattga cttctcggat acgttcgacc tcatctccaa aaaactccac
    46921 acgtaaggca cgttcatctc ttgaagccgg aaaaatctcc acgacatcac cgcgcactcg
    46981 aaaacgaccg cgctgaaaat ctatatcatt tctttcgaat tggatactga ctaagtcacg
    47041 aatcaactga ttgcgatcta gttctgctcc ctgtcgaatc gaaactactt gtttttggta
    47101 ctcaaaaggc gaacctaatc caaaaataca tgaaacggat gcaatgacga tcacatcatt
    47161 tctttctaat aatgaactag tagccgaatg tcgtaatttg tcgatctcat catttacact
    47221 tgaatctttt tcgatatacg tatcgcttga aggcacataa gcttcaggct gataataatc
    47281 ataataagaa acaaaatatt caacagcatt gttagggaaa aattccttga attccccgta
    47341 cagctgacca gccaatgttt tgttatgggc aataattagt gttggtttat tcactttctc
    47401 aataagattg gaaatcgtat atgttttccc tgttccagtc gcacctaata agatttgtgc
    47461 ttttttcccg ccgactacgc catccactaa ttggttaatc gcttcaggct gatcaccagc
    47521 cggttggtat ttcgagacaa gctcaaagtg tcgggaggta tctctctcaa tcattcttcg
    47581 tcctcctttt cattcattcc ttttcatttt acgaccaata aaaagaaaag gaaagcaaag
    47641 gcattcctta ctaaaaaatt ataccacacc gaacatactt tcgccacttt tttatcttat
    47701 tatgcatgtt acgttttata acataaaatg ttagaaactc gcattttttt cattttttcg
    47761 agcttacagt cttgtctgat tcagcacttt tcgcctataa taataaaata ttggaaattt
    47821 aggaggttga ttatgaaaag aaagcattta tttcactcat ttgttttctt aatgacagga
    47881 cttgtgacac ttgcgtttgg acatgttgca caagctgaca acactacaac ggcagagtca
    47941 agcacaagcg agacaatgca aaaaatagag cccaaaaaag acgtatacgt aattgccagt
    48001 gactctgctt ttgctccatt tgaatttcaa aacgcaaagg gcgagtacga aggcatcgat
    48061 gtaaaattga tggaaaacat cgccaaactg caaggattta acatccgaat ggactaccgt
    48121 ggtttctctg ctgctttgca agctttggaa tcaggacaag cagacggaat gattgccgga
    48181 atgactgtaa cagatgaacg aaagaaaagc tatgattttt ctgattctta ctttgatagc
    48241 gggatccaat tagctgtcaa aaaaggagat acttcgatca agtcttatga agatttgaaa
    48301 ggaaaaacag tcggagcaaa agttggtacc gaaagtgctg aatttttaag cgaccatgaa
    48361 tctgaatatg gctattctat caaatatttt gatgctgctg atcaattgta cgacgggtta
    48421 aaagttggcg caatcgatgc aatgatggat gattatccgg taatcggtta tgcgatcaaa
    48481 caaggacaag aattggaaac accgatcaaa aaagaatctg gcggaaaata tgcatttgcc
    48541 gttaaaaaag gacaaaatcc tgagcttctt aatatgttca atgaaggatt gaaagaatta
    48601 aaaagaacag gcgaatacga taagattatt aaagaatatg tagcggatgg ttcagaaact
    48661 tccagtgagt cttccactgc tgatgaatca acatttcttg gacttatcca aaacaactat
    48721 caagcattgt tgaaaggact ttggagaaca attctcctaa ctttgatctc ctttgctttg
    48781 gctatagtaa tcggtgtgat ttttggatta ttcagtgtag caccaatacg cgctcttcgc
    48841 acaattgcta gtatttatgt cgatatcatc cgaggaattc caatgatggt tcttgcgttc
    48901 tttattttct ttgggttgcc tggtatcgtt ggatttacga ttcctgactt ccttgctggt
    48961 gttatcacct tgactcttaa cgcaagtgct tatatcgcag agattgttcg aggcgggatc
    49021 aacgccgttc ctgtcggaca aatggaagct tccagaagtt taggtctttc ttataatcgg
    49081 acaatgcaaa aaatcatttt gccgcaagcg atacgcatca tgatcccatc attcgtcaat
    49141 cagttcgtta tctcattaaa agacacaacg atcatttccg caatcggtgt agtcgaattg
    49201 ctgcaaactg gtaagatcat tgttgcacgt acaacacaaa gtacgtatgt ttacttgatc
    49261 attgcgatca tgtacttgat tttgatcaca gcattgacaa aattagcaaa agttttagaa
    49321 aagaaggtga agtaagatgg cagaaaaaat cttagttgag catttagtca aaaaatacgg
    49381 cgataacaca gtattaaacg acatcaatgt gtccatcaac gaaggagatg tcgtctgtat
    49441 cattggccct tcaggttcag gaaaaagtac atttttgcgt tgcttgaatc gtttagaaga
    49501 accttctgac ggtgaaatta ttattgacgg cgcccacttg atggataaaa gcacagatat
    49561 caacttggtg agacaacata tcggaatggt attccagcac tttaacctat tccctcatct
    49621 ttctattttg gaaaacatca ttttagcacc gatggacctg aagaaagaat ctagagaaga
    49681 agcagagaaa aaagcaatca aattgctgca gacagttggt ctagaagata aaaaagatat
    49741 gtatcctgaa atgctttccg gtggacaaaa gcagcgtgtg gctattgctc gtgctctagc
    49801 gatgaaccct gacatcatgc tttttgatga gcctacatct gcactcgacc ccgaaatggt
    49861 cggtgatgtc ttgaacgtca tgaaaaagtt agcagaacaa ggtatgacga tggttatcgt
    49921 tacgcacgaa atgggcttcg ctaaagaagt ggccaatcgt gtcatgttca ttgacgacgg
    49981 aaacttttta gaagacggaa cgcctcaaca aatctttgaa aatccgcaaa atgaacggac
    50041 aaaagatttc ttgaataaag tattaaatat ttaataaaaa ccaaaaatgc ccggtcgctt
    50101 catgacgacc gggcattttt agtttcttgt tttttcactt aactgtacca gccgcatcgc
    50161 ttgttctcct gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat
    50221 cggttcattg acgatatcaa gtacatagtc gataccacct tcagaaacta tgatgattat
    50281 cttattctaa agctggcttt tttgttggta caaacatgac aactacaaaa aaaccaggcg
    50341 aacgtatgat tgttcgtctg gtttttaatt attaatgact tagcgttttt actagaaata
    50401 gaaaatttgg gcaagtctac tttatatttt tactagaaaa actacttccg ctttttcccg
    50461 ccaaaagcat ctttaaactt atcgaagaaa ccgtcttctt gctgttcttc aactttgatg
    50521 ccgccagctt cagcgaatgc acgtaatgct tctctttgtt gttcatttag atttttcggt
    50581 gtgatcagtt ttactttgac atgttggtcg cctgttgcac caccacgtaa cttcggagcg
    50641 ccttttccac gtaaacggaa gttcgtacct gtctgtgttc cagcagggat cttcaatttc
    50701 acatctccat ggacagttgg tacttggact tcatccccca aagctgcttg tacaaaactt
    50761 aaaggcaatt catagtaaat ttcagaacct tcacgatcaa agatatcact ttcttctaca
    50821 cgaaaaacca catacaaatc accaaatggt ccgccgtttt ctccagcttc tccttgacca
    50881 gctaaacgca tttgttgtcc ttcttctaca ccagctggta cattcacttt aactgaatgt
    50941 gctttcttct catgtcctga gccatgacag gttggacaag gttctttgat ttctttccct
    51001 gtgccatgac aaacatcaca agtttgacgg ctcatcactc gtccaagtgg cgtttggcgt
    51061 tctacattga tcgtacctgc accatgacat ttatgacaag tagttggttg tgtgcctggt
    51121 tttgcaccgt ttccaccaca agtatgacag atttcttccc gattatactt gatttctttt
    51181 tctacaccaa aaatcgcttc ttcaaaagac aagtcgaccg tatattgcaa atcagcacct
    51241 tgacgaggcg catttggatc gactgaccga ccgcctcctc caaagaaaga gtcgaagata
    51301 tcttcaaaac caccgaagcc gccaccagag aagccgccga atccaccaaa tccgccacca
    51361 gctccaccag caccgtaatt tggatctgtg cctgcatgcc cgtattggtc gtatgcagcg
    51421 cgtttttgcg ggtcacttaa aatctcatat gcttctgata cttctttgaa tttttcttcc
    51481 gcatcaggtt ctttattgat gtcaggatgg tattttttcg aaagcttacg ataagctttc
    51541 tttatttcat cttctgatgc tccttttgag agccctaaga cttcataata atcacgtttt
    51601 gttgccatag gctttcctcc tattggttct catcattttc acgtcgcttg ttatcatatc
    51661 atattttgtt tgaaacaaaa accgatactt caaactattt ttcaaaagaa aaagccaaag
    51721 tagcaacttt ggccttctct cgaaattcag tgaactgatt atttatcgtc gccatcgact
    51781 tcttcaaaat cagcatcaac gacatcatct gctccgcctt gagctgcttc tggattttct
    51841 tgagcttgtt gttgtgcagc ttgttcgtat agtttaacag tcaagttttg aacgatttcg
    51901 ttcaatgcat cgcgtttagt tttcatttcc tcgatgttgt ttgcttcaac agcggctttt
    51961 aattcgtcac gagctgtttc tgcttttttc acttcttctt cgtccacttt gccttctaat
    52021 tctttcaatg ttttgtccac agagaatagc aatgcgtcta catcattacg taggtcaact
    52081 tcctctttac gagctttatc tgcttcagcg tttgcttctg catctttcac catacgttcg
    52141 atttcttcgt ctgttagacc tgaagaagat ttgatcgtga ttttttgttc tttttgtgta
    52201 cctagatctt tcgcagatac atttacaatc ccgtttttat caatatcgaa tgttacttcg
    52261 atttgtggaa taccacgagg agctgctgga atatctgtca attggaatct tcctagagtt
    52321 ttgttgtcag cagccattgg gcgttcacct tgtaatacat ggatgtctac tgctggttga
    52381 ttatctgccg cagttgagaa tacttgtgat ttacttgttg gaatcgttgt attgcggtca
    52441 atcaatttag tgaacactcc acccattgtt tcgataccta atgacaatgg tgtaacgtca
    52501 agtaaaacaa catctttcac atcaccagtg atcacgccac cttggatagc agcacccata
    52561 gcaactactt catcagggtt aactgatttg tttggttctt ttcctgtttc tttacgtact
    52621 gcttctacaa ctgcagggat acgagtagat ccaccgacta aaataacttc gtcgatttct
    52681 gattgtgaca gacctgcatc ttttaacgct tgacgtactg gtacttttgt acgttctact
    52741 aagtcagctg ttaattcatc aaattttgca cgtgtcaatg tcatttccaa atgcaatggt
    52801 ccagattctc ctgctgtgat aaatggcaag ctgatttgtg tgcttgatac gccagataaa
    52861 tcttttttcg ctttttcagc agcgtctttc aagcgttgta aagccatttt gtcttgagac
    52921 aagtcaatgc tgttttcttt tttgaattct gctaccatgt agtcgatgat tttgttatca
    52981 aagtcgtccc cacctagatg gttgtcgcca gctgttgaca atacatcaaa gacaccgtct
    53041 cctagttcaa ggatagatac gtcaaaagta ccgccaccaa ggtcgaatac taagattttt
    53101 tcgtcgcgat ctgttttatc taaaccatag gcaagagcag ctgcagttgg ttcattgacg
    53161 atacgttcta cttctagacc agcgatctta ccagcatctt ttgttgcttg acgttgtgca
    53221 tcattaaagt aagctggcac agtgataact gctttttcga ctttttcacc taaatagtct
    53281 tctgcgaatc ctttgatata ttgaaggatc attgctgaga tttcttgtgg tgtatatgat
    53341 ttaccttcta catctacttt gtaacctgct tcaccgatat ggcgtttgat tgatgaaatc
    53401 gtatttgggt ttgtcacagc ttgacgtttt gcaacttccc ctacttggat ttctccattt
    53461 ttgaatgata caacagaagg tgttgtacgg tttccttctg ggttagcgat gatttttgct
    53521 tcgtttcctt ccaatactgc tactgcagag ttagtcgtac ctaagtcaat accgataatt
    53581 ttactcataa ttaacgtctc cttctttaat tgaaagtgta agttttatat ccgcacaatt
    53641 attgtgcaac aatgaccata gttggtctta acacgcgatc atgtaatttg tagccttctt
    53701 gtagtacttc tacaatcgta tcggctggtg tatcttcagt cgctggaaca gtttgtactg
    53761 cttgatgaag attaggatca aaggcttctc ctttagcagg gattttttca attccttctt
    53821 cttccagtgc gttacgcaaa ctttccaaga ccatttccac gccttttttc aaacctgccc
    53881 cttgatcatc tgacacttct gtggctaatg cacgttccag gttgtcaatt gatggaagta
    53941 attttttggc taaatcttgt gaacggtatt tttgtaattg ctcacgctca ttttttcctc
    54001 gatttgccat atttgcgatt tcagcacgag cccgcaagta tttatcttcc atttcttcta
    54061 actcagcttt taatttcgct gtttcgaatt cttccgcttc agcttctgaa atacccgaat
    54121 cttctgcagc ttcgacatcg atttccggtt ctggctgagc atctgtcatt tctttatcta
    54181 gttcttcttg attttctttc acgatacagt cttcctttca ttattcagtc agctgtttga
    54241 ttgctcttac taagaaagag agaacgatca gctgcaaata gcttcgttat tcacattacg
    54301 agtttgacaa gtcaagcgag cggtagtaat cagcaagttt agctgctagt tctcttcgat
    54361 aaacatcgac tagaccgaac atccgagaat aaggcatact agtaggccct aacaaggcta
    54421 ttgtacctct tccatgacca tctatctcgt aacttgcttg aatcatactc atattctgca
    54481 ataaatcatt gcctaattca gcaccaatct tgatatgaat attgctatcc atcggagtga
    54541 tcatttgtgt aagtccgtct gagtctttca taaaagaaaa catcgatttg aactgatcaa
    54601 gatcttgacg gggctcataa tccaagagat tcatttgtcc actgacataa accttttctt
    54661 caaaaacatg accaagaacg gtgtcaaata actgagacat cccttctgtc gtctggaaat
    54721 atttatgaag aatcatcgga atctccgtac gaagtttatg gtacaccgtc atcagaggat
    54781 ctccgactag acggtcgttt acaatctgta ccattttttc tagttcctga cttcccatat
    54841 attcgggaag agtgaacact tggctttcta cattccccat atctgttacg acgatagcaa
    54901 taatctgtcg attattcaag ggtacgatac gaaagccagt cagtcggcgg tcttttattt
    54961 caggtcccaa tgaaaaagtc gtatagcttg tcagctcaga taatatctca gcagattgct
    55021 taataatctc attgatttca tgaaattctc tgcctagaga ctgacgaatc agcagcaaat
    55081 catcttctgc tacgcgagca ggttttaaca aatgatccac gtaataacga tagcctaaag
    55141 cagatggaat ccgcccagaa gaagagtgag tcttcaacag caatccttca tcttctaatg
    55201 ctttcatatc attgcgaatc gttgctgagc tggccttgac tccctcagac attaatgttt
    55261 tggaaccaac gggcacgcca ctactcgtat agtgttggat gatcagacgt aaaatgtcgt
    55321 tttgtcgctt tgttaacatc ttcctcacct ctcattagca ctcatatatt ctaagtgcta
    55381 attcacttat aaatatagca agaagccctt ctcctgtcaa gagaaagact ccatttttta
    55441 gcactctaaa cacaagagtg ctaattctaa ataattgatg taaatccagt tatatcaata
    55501 cttcaatacc tcattgtcaa actttctaaa ctttttcttt tcaagtttcg ataaaaaaca
    55561 aatattcttc aaactatttt tcaaataaaa agcgctcaaa cacattgttc cccaaaaaag
    55621 tccctcttgg tgtcaaataa acacggtctt cttcattaac aagtaaccct tcttgctcta
    55681 attcccttag cacatcaccg tacaccgatt caattgtttt ttgatagcga tcatagaaat
    55741 gttttttagt aatccctgtt ttttttctta gtcctaagaa catttcttct tctatttgat
    55801 tttttcgtgt caatttttct tcttccaata ccgggcgttc atttgctcgt aatgggcgta
    55861 aatagtgttg gattggtcct ttgttgcggt aacgaatatc tccaagataa ccgctagcac
    55921 cagctccaaa cccaaaatag tggtcattgt tccagtacat gaggttatgt tgagattcat
    55981 gtccggagag cccaaagtta cttatttcat attggtgacg tccatgcttt tccattgctt
    56041 caatcgtctc ttcaaacatt ctggtttccg tttcctgatc aggcagttcg agtcttcctt
    56101 gtcgtaccca gttcatgaac attgttttgt tttcgaggat caatgagtac aaagaatagt
    56161 gaggcagatc caaagccaaa gctttttcca aagtatcccg atttccttct agtgtttgtc
    56221 ccggtaatgc ataaatcaaa tcgatgctta cattggtaaa attttctgct tctaaaaatt
    56281 gcattgtttg atacacatct tctgcagtat gtttacgtcc aatttttttc aataagcgat
    56341 tgtcaaatgt ctgcactccc atggataaac gattgacgcc atagtttttc atcacttgaa
    56401 gtttttcacg ggtcaaatca ccaggattcg cttctacagt aaactcatta ccattttgaa
    56461 aaggcaggat ctctctagca cctgataata gtcgatcaag ttgtttagca gataaagaag
    56521 ttggtgttcc gcctcctaca taaagtgttt ccaaattcgc tgtaggatga ctttccagca
    56581 ttaatctcat ttcattcagc agcatttcca catactcatc tactggctgc ccttctaaaa
    56641 aaactttatt aaagtcacag taatagcaga tatgctcgca aaatgggata tgaatgtacg
    56701 cagaaacctt gttttctgcg ggatttattg tttctatcat tgaaattctt tctccttacg
    56761 tcgaaaagtc gtgcctgttt tcgaccttct taaaatcgcg ttcaagcacc aactcattga
    56821 aataagccgc atatttagaa tcaaggaaac ctagctctaa aaatgcttgc ttattgtcat
    56881 cggagctaca cggtgctttt acagcctctc gataaacggt aatcgcaaag ctgaccttcg
    56941 gcattaagtc aaaatctgca aaaatatgaa aagctatttt cacagattcc tccttaagtg
    57001 ctcagaccac aacgctttgc ttacaacctc tcatatacgc atactaaacg tatttggcga
    57061 acaaatccac taaaaaatcc aagaatcgac ttcttggatg atttttttac actcatgatt
    57121 cttcctaaac aattttgtcc ttattttatt cataaaaaaa tctagtcaaa tctctcactt
    57181 caagagtttt aactagattc caattattat ttttttgtaa aaataaacag tctcttaatc
    57241 atagacattt cttcggtgcc ctacttgaat agcgataaca acgaattgat tatcttctat
    57301 ctctaccaat acacgtattt gccaatcaga gctgcttctg aatttcgggc gtgaatttta
    57361 aacggcaggt cattccagcc caccaaattc attaaggatt cttccatgaa tactgttgtt
    57421 ttcccacttc ttatccaatg tttatgggca atttcagccg tttgacgatt gtattcatct
    57481 tctagttgtt ccatcgaata gttttttaga agttcagata aagtaatccc ataaaattca
    57541 gccatataag aaagccattc ttttttggcg tcacttacac gaaccgtaat catagcccta
    57601 taattgcctc tcttaaagaa tctcatgcat ttcctgatgt gattttagaa aaatttactt
    57661 gctaaaaaac tgtgaaaatg taggagctag catccttgta gtaaatgatc gagctcttta
    57721 ttatacatta aaaaagttca gaaattttga tgaagtagcc aactactttt tcatgagtaa
    57781 agcaacacta gaaaaagaat cctccagtac ttagaattta ctggaggatg tacttttgaa
    57841 gaagcctata ggttatttaa aaaatattac tacaggaagg aacgagaatt acttcgttac
    57901 ttataataga acttagtctt atatagaagg catagctcac attagcttat ttgtcctata
    57961 aaaataaatt acggtatttt agaaaagcta ttttctcttt tttcaacgat cttcagcaat
    58021 ttttctttat cgtcaggtaa ctcaagttca tgaattctag catgacaatt gggacataac
    58081 gcgatacagt tttcaatcac atcttttcca ccttgcgaca aatattctat atgatgacaa
    58141 tgtaaataag gtacaccgtc ttttataaat ggcccttttt cttggcaaag ttgacaaata
    58201 ccttttgcaa gtcgtttaac atattcagat acgaagggat tacgtttata tatttttgtt
    58261 tttgagaagc aaaactcctc ttcgttaaca tattttgcag ccattttttt attataatta
    58321 ctaacttttc ttgcttcttc ctctaattta gcattagata aattacgaat atgtctagtt
    58381 tgattttctt cctggttata tatctttatc ttcaatcaat tgctcttgat ttattaaagc
    58441 taatggaaat ttatatactt ttctgctgtt gcccgaacta tctttttcat taacagtata
    58501 tatttgtcct gctaaatata cttctcccag atatgtgtgt tcttttggtt taaatgtttc
    58561 aaataaatga atctttacaa aattactttt tgaattggct aaaactttat tttgattctt
    58621 atccaatact tgatcccctt ttagccccat accggtatat tgtaaaatac catttaacca
    58681 cttatcttta taaacattac ttcctgtttg atcactaaac agtacaagag tattagtttg
    58741 atgagatctt ctcattccac cctgtttgga acatttaaac ttctctgaaa tttctttgtt
    58801 agtatatact ctaccaactt ctaactcacc taatatgttt ttagaccaat tttctcgcca
    58861 tgtaataatc ttatcaagat taaacatttc tccctctttt ggcatgccat ttctaattaa
    58921 attagctaat acaccagtag aaatatctaa tttttttagt aattctttct tagtaatcaa
    58981 tgttggattc ataacaatgc agctcctaat gatatcaata taattgcatt agataacttg
    59041 aaaaatcgaa aaaaactcat tttgtgtcaa gttatctgta atatataaaa atatcattat
    59101 tatacctcat ctatgtgaat ttttactatt cttaccttag catattcttt ataggtatat
    59161 cagagctaat caaactaaaa aatacccgaa gtcttcttat gtaaaaacac cggttattac
    59221 ttaaatatga cttaggtaaa acaatgagtg atccaattat aaaattttat ggcgaataat
    59281 ttagtaagcg ctacatattg aatgatttag aaattttgaa aaagtagcta gttcctttta
    59341 taccagaaaa gtaaacctaa taaaaaacac aactaatttt tagctgtgtt ttttaagtta
    59401 caataattat ctatttaata gtcttaattc attaaaataa aaaactcaac tttacaagga
    59461 tatggtttaa aaaatttatt tatccaaccc aactagtttt cgctgcttac gatttgcttc
    59521 atctgctacg taattcatta ctgttttaaa ttctttcgct gaggcgtttt ctgataaatc
    59581 agcgatactt ttattatact tcttcacgat ttgttgtact tgttctttct cagtcatgta
    59641 attcctcctc caactctttt gaagtatatt gtattggtag tactaattga atattatctg
    59701 ctgcataatg cacttcagaa atgtcgtttt gataatcctt aatcattatg tgaaggacta
    59761 cttcaggatg tctatttttg actttttcaa tattctctct ggacttaaaa aacgcattga
    59821 taaacatttc ttttggcaca tgacgtcctt cagctagttc acgttttcga gtgaaatccc
    59881 aggcaacttt gggatcttgg taaacataga aaatcgtaac gcgaaagtta tttttaaggg
    59941 ctcgaataat atttttctct gcactaagga tagcaaaagt atcctctagt atgaaagaat
    60001 agccatgttc cagtacatat tttaatgatt gttcaactaa ccaagaagaa gctttctgaa
    60061 aaacacttga gttctttcca ttgtattctg gaaattgtgc tctaaaatag tccgcatcaa
    60121 tcacaacatg gttgtcataa ttttaagcta aacctagtgc aacctctgtt ttacctgctc
    60181 ctgggcttcc agccatgaat accgcatctt tttcatcatc agggattttt cccttgatga
    60241 ttgtttcaat aaatttatct ttattgaccc tagcgtaatc taaatacttg tctttaggtt
    60301 ccaagattgc accttctttc cttcttctat attcatacta attatacgat atttatataa
    60361 aaaatataac ggtcatttta accatattaa catttagtca cagtaatagc agatatgctc
    60421 gcaaaatgag atatgaatgt acgcagaatc cttgttttct acggcattcg cttttttttc
    60481 attggactag ttatatattt ctagggttgt tttcatgtct gagtgcccga tatgatttat
    60541 ttttcgtttc tataattact ttttgccttc cagtttcttt taagattgta ctcaagcaag
    60601 tgggaattgg ttcttttgtc atccatgtat gcaaaatgtt ataatgctgg taaacaaaaa
    60661 tgattacctg tcatttttat ttagttcgta tttaattaag ttgactgtct gtatactctg
    60721 tctaaaactt aagaattgtt gttttcgctg tcgctatcca aatttaatga atgtctgtat
    60781 gttaaaactg ttgtccttgt catagatagt aaaggagagc ttgaaaatga aaactgcgat
    60841 tacgtttcta ttagaggaat atgccgattg ggaaggtgct tatattacta gcacactaaa
    60901 ccaaaaagag ggatggcaaa acaaaactgc ttctatccaa tcagaggtca cttccattgg
    60961 aggattaaaa acaaaaatcg attacttaat cgaacaaata cctgaaaaag ttgacctttt
    61021 tattatgatc ggtggcaatt catgggacat aaaaaatcat catttatatc aaataatcga
    61081 taaattgtta aaaacagacg taccagtagc tgctatttgt ggggcagttg attatttagc
    61141 tcagagcgga ttattgcaag gttacaaaca caccggaaat gcgcaatttc cttggttaga
    61201 atcggaaaat tatcagaata aagaagattt tatagaacaa caagctgtga aagatcgtaa
    61261 tttgattact gcgaatggaa cagctcctat agaattcaca gacttgattc ttcaagcagt
    61321 ccattttgaa acagaagaaa taagagaaaa aaagattcgt ctatacaaac ttggattcta
    61381 tgattactat aaaaagtacg gagatccttt ctcctaatca ttctcttcaa ttaaatactc
    61441 cttatcaata gtatgacaag taattttact gatggttatt tgtcacactt tttctgacat
    61501 ttatcaaaga agatacactc tatttatatt catagatgga atggcaacag tttttttgac
    61561 aaattttata aggtgcagaa cttctttccg tatgctattc cgattgtcct tgacaatgag
    61621 cctcctcgga tgtgacgatc ttcggcagga gctaacagtt aaagttagac tgcctcgcta
    61681 gcgttagcac atccgagctc attatcaagg attcgctgcg ccaatctct
//
LOCUS       DACDIH010000010        61172 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDIH010000010 DACDIH010000000
VERSION     DACDIH010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.010"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..203
                     /locus_tag="IV281_000616"
     CDS             <1..203
                     /locus_tag="IV281_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP8779653.1"
                     /translation="DFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEENHITASKY
                     HLDDAFFSKSWQHQIPDKLQLK"
     gene            344..1426
                     /locus_tag="IV281_000617"
     CDS             344..1426
                     /locus_tag="IV281_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP8779654.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            1426..2229
                     /locus_tag="IV281_000618"
     CDS             1426..2229
                     /locus_tag="IV281_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8779655.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            2299..3039
                     /locus_tag="IV281_000619"
     CDS             2299..3039
                     /locus_tag="IV281_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8779656.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            3050..4054
                     /locus_tag="IV281_000620"
     CDS             3050..4054
                     /locus_tag="IV281_000620"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP8779657.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(4099..5943)
                     /locus_tag="IV281_000621"
     CDS             complement(4099..5943)
                     /locus_tag="IV281_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779658.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            6155..6673
                     /locus_tag="IV281_000622"
     CDS             6155..6673
                     /locus_tag="IV281_000622"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8779659.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            6801..7484
                     /locus_tag="IV281_000623"
     CDS             6801..7484
                     /locus_tag="IV281_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP8779660.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            7793..7866
                     /locus_tag="IV281_000624"
     tRNA            7793..7866
                     /locus_tag="IV281_000624"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7827..7829,aa:Arg,seq:tct)
     gene            8025..8384
                     /locus_tag="IV281_000625"
     CDS             8025..8384
                     /locus_tag="IV281_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP8779661.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            8507..10138
                     /locus_tag="IV281_000626"
     CDS             8507..10138
                     /locus_tag="IV281_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP8779662.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(10200..10841)
                     /locus_tag="IV281_000627"
     CDS             complement(10200..10841)
                     /locus_tag="IV281_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8779663.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            11123..11431
                     /gene="rplU"
                     /locus_tag="IV281_000628"
     CDS             11123..11431
                     /gene="rplU"
                     /locus_tag="IV281_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP8779664.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            11458..11802
                     /locus_tag="IV281_000629"
     CDS             11458..11802
                     /locus_tag="IV281_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP8779665.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            11815..12108
                     /gene="rpmA"
                     /locus_tag="IV281_000630"
     CDS             11815..12108
                     /gene="rpmA"
                     /locus_tag="IV281_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP8779666.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            12515..15349
                     /locus_tag="IV281_000631"
     CDS             12515..15349
                     /locus_tag="IV281_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8779667.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            15540..16031
                     /locus_tag="IV281_000632"
     CDS             15540..16031
                     /locus_tag="IV281_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP8779668.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            16134..17123
                     /gene="manX"
                     /locus_tag="IV281_000633"
     CDS             16134..17123
                     /gene="manX"
                     /locus_tag="IV281_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP8779669.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            17156..17959
                     /locus_tag="IV281_000634"
     CDS             17156..17959
                     /locus_tag="IV281_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP8779670.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            17980..18894
                     /locus_tag="IV281_000635"
     CDS             17980..18894
                     /locus_tag="IV281_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP8779671.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVERWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            18977..19351
                     /locus_tag="IV281_000636"
     CDS             18977..19351
                     /locus_tag="IV281_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP8779672.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            19490..19735
                     /locus_tag="IV281_000637"
     CDS             19490..19735
                     /locus_tag="IV281_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8779673.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            19907..20971
                     /locus_tag="IV281_000638"
     CDS             19907..20971
                     /locus_tag="IV281_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8779674.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            21217..21642
                     /locus_tag="IV281_000639"
     CDS             21217..21642
                     /locus_tag="IV281_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8779675.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            21635..22084
                     /gene="nusB"
                     /locus_tag="IV281_000640"
     CDS             21635..22084
                     /gene="nusB"
                     /locus_tag="IV281_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP8779676.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            22181..23035
                     /locus_tag="IV281_000641"
     CDS             22181..23035
                     /locus_tag="IV281_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP8779677.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            23036..24373
                     /locus_tag="IV281_000642"
     CDS             23036..24373
                     /locus_tag="IV281_000642"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP8779678.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            24378..24602
                     /locus_tag="IV281_000643"
     CDS             24378..24602
                     /locus_tag="IV281_000643"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP8779679.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            24604..25494
                     /locus_tag="IV281_000644"
     CDS             24604..25494
                     /locus_tag="IV281_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8779680.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            25503..26321
                     /locus_tag="IV281_000645"
     CDS             25503..26321
                     /locus_tag="IV281_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP8779681.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            26428..26877
                     /locus_tag="IV281_000646"
     CDS             26428..26877
                     /locus_tag="IV281_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8779682.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            26895..28577
                     /gene="recN"
                     /locus_tag="IV281_000647"
     CDS             26895..28577
                     /gene="recN"
                     /locus_tag="IV281_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP8779683.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(28632..28748)
                     /locus_tag="IV281_000648"
     CDS             complement(28632..28748)
                     /locus_tag="IV281_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP8779684.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(28821..29777)
                     /locus_tag="IV281_000649"
     CDS             complement(28821..29777)
                     /locus_tag="IV281_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8779685.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            29948..30316
                     /locus_tag="IV281_000650"
     CDS             29948..30316
                     /locus_tag="IV281_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP8779686.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            30486..30917
                     /gene="mraZ"
                     /locus_tag="IV281_000651"
     CDS             30486..30917
                     /gene="mraZ"
                     /locus_tag="IV281_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP8779687.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            30938..31897
                     /gene="rsmH"
                     /locus_tag="IV281_000652"
     CDS             30938..31897
                     /gene="rsmH"
                     /locus_tag="IV281_000652"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP8779688.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            31902..32303
                     /gene="ftsL"
                     /locus_tag="IV281_000653"
     CDS             31902..32303
                     /gene="ftsL"
                     /locus_tag="IV281_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP8779689.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            32304..34496
                     /locus_tag="IV281_000654"
     CDS             32304..34496
                     /locus_tag="IV281_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8779690.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGMFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            34522..35484
                     /locus_tag="IV281_000655"
     CDS             34522..35484
                     /locus_tag="IV281_000655"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP8779691.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            35498..36859
                     /locus_tag="IV281_000656"
     CDS             35498..36859
                     /locus_tag="IV281_000656"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP8779692.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            36885..37973
                     /gene="murG"
                     /locus_tag="IV281_000657"
     CDS             36885..37973
                     /gene="murG"
                     /locus_tag="IV281_000657"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP8779693.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            37988..39208
                     /locus_tag="IV281_000658"
     CDS             37988..39208
                     /locus_tag="IV281_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP8779694.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            39364..40689
                     /gene="ftsA"
                     /locus_tag="IV281_000659"
     CDS             39364..40689
                     /gene="ftsA"
                     /locus_tag="IV281_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP8779695.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            40712..41953
                     /gene="ftsZ"
                     /locus_tag="IV281_000660"
     CDS             40712..41953
                     /gene="ftsZ"
                     /locus_tag="IV281_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP8779696.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            41970..42647
                     /locus_tag="IV281_000661"
     CDS             41970..42647
                     /locus_tag="IV281_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP8779697.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            42661..43260
                     /locus_tag="IV281_000662"
     CDS             42661..43260
                     /locus_tag="IV281_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP8779698.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            43275..43562
                     /locus_tag="IV281_000663"
     CDS             43275..43562
                     /locus_tag="IV281_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP8779699.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            43611..44393
                     /locus_tag="IV281_000664"
     CDS             43611..44393
                     /locus_tag="IV281_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP8779700.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            44414..45121
                     /locus_tag="IV281_000665"
     CDS             44414..45121
                     /locus_tag="IV281_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP8779701.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            45383..48181
                     /gene="ileS"
                     /locus_tag="IV281_000666"
     CDS             45383..48181
                     /gene="ileS"
                     /locus_tag="IV281_000666"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP8779702.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(48230..49756)
                     /locus_tag="IV281_000667"
     CDS             complement(48230..49756)
                     /locus_tag="IV281_000667"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP8779703.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(49771..50418)
                     /locus_tag="IV281_000668"
     CDS             complement(49771..50418)
                     /locus_tag="IV281_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP8779704.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(50602..50931)
                     /locus_tag="IV281_000669"
     CDS             complement(50602..50931)
                     /locus_tag="IV281_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP8779705.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            51108..51836
                     /gene="sfsA"
                     /locus_tag="IV281_000670"
     CDS             51108..51836
                     /gene="sfsA"
                     /locus_tag="IV281_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP8779706.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            51852..52865
                     /locus_tag="IV281_000671"
     CDS             51852..52865
                     /locus_tag="IV281_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8779707.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            52865..54142
                     /locus_tag="IV281_000672"
     CDS             52865..54142
                     /locus_tag="IV281_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8779708.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            54205..56907
                     /locus_tag="IV281_000673"
     CDS             54205..56907
                     /locus_tag="IV281_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8779709.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            57059..57376
                     /locus_tag="IV281_000674"
     CDS             57059..57376
                     /locus_tag="IV281_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8779710.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            57406..57726
                     /locus_tag="IV281_000675"
     CDS             57406..57726
                     /locus_tag="IV281_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8779711.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            57834..59294
                     /locus_tag="IV281_000676"
     CDS             57834..59294
                     /locus_tag="IV281_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8779712.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            59362..59583
                     /locus_tag="IV281_000677"
     CDS             59362..59583
                     /locus_tag="IV281_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779713.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            59614..59796
                     /locus_tag="IV281_000678"
     CDS             59614..59796
                     /locus_tag="IV281_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP8779714.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            59796..60209
                     /locus_tag="IV281_000679"
     CDS             59796..60209
                     /locus_tag="IV281_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP8779715.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            60332..>61172
                     /locus_tag="IV281_000680"
     CDS             60332..>61172
                     /locus_tag="IV281_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8779716.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYM"
BASE COUNT        20218 a        10299 c        13128 g        17527 t
ORIGIN      
        1 ctgattttgt caatcaagcg ggtaccaact attttatgga agacacgatc catctaggct
       61 ggaaaggctg gttggctgca gatcaacaga tcagaccgtt tttagaagaa aatcatatta
      121 ctgctagtaa gtatcatctt gacgatgctt tttttagcaa atcttggcaa catcaaatac
      181 cagataaact acaattaaaa taaagaaaac aggcgagtgt tgctcgcttg ttttctttat
      241 ttttgaatat tctgaataaa agataaaaat accaagtttg cctgaacaaa aatcataagc
      301 aatggtataa tcacaatatg aatatagaaa tgggggaaaa catttggata caaaagaaga
      361 acagctactt attgatttaa cttctgcaaa aggagtacct ggaaatgaag aagaggtaag
      421 agaagtattc agagaatatg ccaaaccatt cgctgatgat attttttatg acggtttagg
      481 cagtgtgatt gccaaacatg gtgctggcgg aggccccaag gtctttattt ctggacatat
      541 ggacgaagtt ggttttatgg taacaaaaat aaccgaaaaa ggattcttgg aattccaaac
      601 tcttggaggt tggtgggggc aagtcatgct tgctcagcaa gttgaaatca aaacacgtga
      661 agggaaaatc gtccacggtg tgattggttc caaaccacca catgttttaa cacctcaagt
      721 aagaaataaa ccttatgaaa taaaggatat gttcattgat attggcgcat caagccaaga
      781 agaagccaaa gagtggggca tacgtcctgg agatatggtc actccatata ttgaatacaa
      841 acgaatgaat ggcagtaaat atttactggc aaaagcatgg gataatcgta tcgggacagc
      901 tgtttctctg cgagtattag agaatctatc taaagaagca cacccaaatg ttttattcgc
      961 aggaagcgat gtgcaagaag aagtaggctt gcgaggtgct cgaacaagta cacaccttgt
     1021 aaatcccgat attgcttttg ctttagatac tggtacagca ggagacactc caggaatgac
     1081 accaaaagaa gcagattcga ttttaggaaa aggcccacag attttgattt ttgatgcatc
     1141 tatgatcccg cataaaaaat tattgaattt tgttattgat gtagcagagg aaatggatat
     1201 tccattccaa tatacagtga tttctggcgg aggaactgat gcaggacaaa tgcatttgac
     1261 aagagatggt gttccgtctg tagccattac cgttcctgtt cgttatctgc attcccatac
     1321 atcagtgatt catgaagatg attatttgaa tacagtgaaa ctagtcacag aattagtgaa
     1381 acggcttgat agtgaaacag ttgaaaaaat tcggagttat gaataatgaa taatagaata
     1441 cctatttctg aatatcggaa aaaagcacga gtttctttgg aaaatcaatg gggaatcaat
     1501 acttggcttg ttttcttatc tataatattt acgagcgtca tccagaactt ttttgacggt
     1561 ttcagtcctt ttccggaaga aagcatgcag cagcacttat tgagttttct agtcgataat
     1621 ttacttttat tcgcattcaa ctatgcattg ttttacattg cgctagtagt tgtacggggt
     1681 ggcagaccaa aaagtaacct tttattttca gttttccaaa gaaagtattt tggttcgata
     1741 cttggcatca atttgatcaa ttcagtcatc aactggctac tcaacagttt aatctttctg
     1801 cccatgtttt taatcagcgg agtgagtact tatatgcagc tgcttttcag ttcaaatgcg
     1861 gtacctacgg gtgtcatgcc agaagatttt cttgatatca gctttgtttt atcgatgata
     1921 gggatgattt ttgtttcagc actcatcatg agtattgttg gcggtttatt ccaatttgct
     1981 gcatggacaa agttcgacta tcctgatttg tcggttattc aatgtttgaa atatggctgg
     2041 tttttattga aagatagaat tggtacgtat attttgttgc agctgtcttt tatcggctgg
     2101 tatttgctag gagcaatcgc tttgttcatc ggtcttttgt gggtcattgt ttatgtgaat
     2161 gtgtcagttg cagaatttta tgaacaagca cggattgaaa aaggaggtcc agacgcctat
     2221 tttacttagc atgtaagcag tttattcatc aaaaaaataa cttttgtggt aggataaatc
     2281 tttgaggggg aatgtacgat gaaattaact gttttaggat gcttaggagc gtatccttat
     2341 aaaaatcaag ggacaacagg ttatttgctt caatctgacg ggttcaattt gttgatcgat
     2401 gcaggtagtg ctacgctgat caaattacaa gaacaccttg atccacttga tctagatgct
     2461 gtgatcctta gccattatca tcatgatcat attgctgacc ttggtgtatt acagtattac
     2521 tggcaacttc atcctaagag aaatccggag acattgcttt caatttatgg acacacaaaa
     2581 gattccattc actttgaaga attgacgttg gaaaatgtct caaaaggaca tccttatttt
     2641 gaagctgaag aattaaaaat tggtcctttt ctgattactt ttatggaaac aatccatcca
     2701 gtagtatgtt acgccatgcg tatcgtagaa gaaacaacgg ggaaagtatt cgtatttaca
     2761 ggtgattcag gttatttgga aagtttcgct gattttgcga aagatgctga tttgtttcta
     2821 gcagatacct atctttttga aggaaatgaa catcatcatg cccatttcac ttcaaaagaa
     2881 gctggagaac ttgcgaaatc agctaacgta aagaaacttg tacttacaca cctacctcag
     2941 tttggtgatt tagaacaatt gaaaaatgaa gcgcaaaagg cagcaggcaa agggattgat
     3001 atagaacttg ctgcagttga caaagtgttt aacatttagg aggaagaaca tgatttttgt
     3061 accaaatgaa aaccacgacc caagaatcaa tttagcaatt gaacaatatt tgctgcaaga
     3121 aatgccttta gatgaaccta ttttgctctt ttatatcaat gaaccttcaa tcatcatcgg
     3181 cagaaaccaa aatacgcttg aagaaatcaa tcgagattat gtggaagaaa atggcattca
     3241 tgttgtccgt cgcttaagtg gaggcggagc tgtctaccat gattttggaa acctgaactt
     3301 tagttttatc atgcctgatg atggggattc attccgtgac tttgcgaaag taacacagcc
     3361 aatcatccaa gcacttcatg aattgggtgt tgctggggcg gaattgaaag gccgaaatga
     3421 tctagtcatt gatggcatga aattttctgg caacgcgatg tatgctacca acggtcggat
     3481 gtttgctcat ggaacaatta tgttcgactc tgatatcaat gaagtagtca atgcattaaa
     3541 agtaaaaaaa gacaaaatcg aatcaaaagg aatcaaatcg atccgttctc gtgtaacgaa
     3601 tatcaaacca tttcttccag aagaaaagca aaaaatgaca acagaagaat ttcgtcaaga
     3661 aatcttgttg aaaattttcg gcgtagagtc agtagaagaa gtaaaaacgt acgaattgac
     3721 tgatgaagat tggaaaaaaa tcaatcgaat ctctgaggaa tattatcgaa attgggattg
     3781 gaattacggg aagtctccag atttcaattt ctctcgccaa aaacgttttt ctatcggttc
     3841 gattgaagtc catttgaatg tctcagaagg aatgatcaaa gaagcaaaaa tctttggcga
     3901 tttcttcgga ctaggagata tctctgacgt ggaaaaaaaa ttggttggac agaaatatga
     3961 taaagcatct ctatcaaaaa tagtcagcca aattgatgtg aagaaatatt ttggtgcgat
     4021 agaaccagaa gaattgctta ccttacttta ttgaaaataa aaaatagcgg aatccacata
     4081 tatggattcc gctatttttt atacaggatt cgggtaattt cgagagagtt gaacagtgat
     4141 ccgatcaatg aaagtatctt tttcctgcgt tagtgttcct gtgtaacgat ccgctacgtc
     4201 tttcatcatc attaagtcaa aacgttcctg atcatctaat tcttctgtga tgcctcgact
     4261 aaataggttt gtatcgtaat gtgcactatg ttcgcaacaa atgacgaaat gttcctcttg
     4321 ttcgtgtatc gacaaataaa tttttcgata cagtggattt tcgatggcat gagtttctct
     4381 aaaggaatgc tctaaagcgt ggatcaaaag ttgagccaga tcgtcatcgt tgataggaag
     4441 ttcttcggga agatctgctt ggaaggttat ctgaatattc cgtttggcag ctaacttctg
     4501 gtaacgcgct aagataagat ttgtcacctg gtggttgcaa aaagaacaca gcatttcgaa
     4561 attttttgct tcttctaata tcttttccac atagttttta gcttttgttt ggtccgtatg
     4621 cattaaatct tgcagattct ccatctcttg gacaaattcc aggcgaagtg tatttacttc
     4681 ttggatatgc tgtcgaagct gctcataatg ttgctccaac aaatcatttt tgatttttaa
     4741 gaaatctact tgtctccgat ctttgtcggt atttaagatg tgatcgatta gattgtagct
     4801 gataataacg attagaatga taatatataa aatcgtagac caattgatcg ttgtttgata
     4861 gatacctaat gccgtttgga tgtaaatcaa gcaatggata atggctacca agacgatcca
     4921 aggtgtacaa gctacataaa aacggttatt cttatatgct tcgccaatgc atgcaagtga
     4981 agtgactaaa gtgcttataa cactagcaat attagcaatc ggcattaatt caggaagttc
     5041 tgcaggagta aaagttgcat aaagcagaac gccgataaaa atcaaaagtt ggaacgtaca
     5101 aaaaattccg taccattttt tatagtaaaa cattcggctc caatagtagc agatgatgaa
     5161 agctgacgtt aagataataa ataaatgaga taaaaaagaa tgaacgaatg gttcaaaaag
     5221 aataccactt aaaatatctt ctgatacagc ttcaaatcct aatgccaaag caaagcaact
     5281 taagagaata agtgagtatt caagacgttt ttgtttatac atcaagtaga tcgtatagaa
     5341 aaaggtccct atagcgataa aaacagcgat tatcatcgtc aatacttgcg gcactgattg
     5401 tgaataaata aaactaatca atggtccagt tgttcctaga taaactttcg gtggcagtcc
     5461 agcatagttc tgataaggag atgagactgt aagggtcaat ttttttccga tataatttgc
     5521 tggtaaatct atcacagcca atgagagtcc gggattgctt tcttcctgat aatctttccg
     5581 ttggtataaa atcttttcgc ctagatatac agtaatccat tgatggtttc ctttgattag
     5641 taaatttgct tcgtccaacg gtttttcaag tgtcgtttcc ataatgaatg tttcgtcttt
     5701 atccacggta ggaatcgtac gagtgtactt gctgtcaaac ttaaaatcag gatcatcttt
     5761 tgtatagaaa acccacgaat cttttatttc tttcatttga ttgctgtccg caggagaaaa
     5821 aataaaataa aagaaataag caattcctgt taaaaataac aatgataata taatgattat
     5881 gctccacttt atacgtaaat aaaaagattc ttttttatta tatatgttat taaaaaaaga
     5941 cataaaggta ttcctttcta aaacttgtaa tgttagggta aacgtttttt aaactaacgt
     6001 caattattta taataaaaaa tgagataaag atgaggaaat agaattagaa acatataatt
     6061 attaaaggga aacattttta cagctataat ttgaaaaaaa tttatcgtta ttatatgatt
     6121 aagaaggatt gaataaagaa agaagttgaa aacgatacct gattacaaaa aaataacttt
     6181 tataagttct ataacgttga ttatattttt aattcttatt cttctcgtcg tcttggttcc
     6241 ccgaattttt ggtttgactc cttatattat tgaggacaaa gaaatgagtc catattatgc
     6301 acaaggaaat ctgatatatg tacgaccgaa aaatacaaat gagattttag tcggagatat
     6361 tattacatat tatgaaaatt caggaaaaaa aatctcgacg cgtcgcgtaa tagccgttga
     6421 aaacaaccac gaagattttt atacaaaagc tgatacaaag acttatattg agcctggagt
     6481 agtaaaaaag agaaatatta tcggtagtcc catctttcag attccatata ttggactagt
     6541 cctttcaaaa acggcatttg catggattca ggggttattc tttacgattg ctttttgcct
     6601 aacaggaatc acagcatgga atacctttgt agagttgaaa aagaagcgga gacgaacaca
     6661 gaaatttttg taagaatgtt aaaaacataa atgtattcca gagatccaac gactcttctg
     6721 aacatgaaac acgagaaaac actcatgcag cttgtagatt tagtcagttg ttcgtataat
     6781 gtgtgaagag gaggagtagt atgcgcaatg aaatgatgca tcgtcctgta gtcaaagaag
     6841 aactattgga ttttatgcgt acccagcaaa aaaaattacc gggtgagcta ggcaaactgg
     6901 aagaagaagc tcatgtagca gaagtaccag ttattcccca tgaaacagtt gtatttttga
     6961 aatttttact tggacagctt cagcctgaac gaatcttgga gatcggagca gctatcggtt
     7021 tttcatcaag tgtcatggcc acgacaatcg gagaaaacgc tcatgttacg acaattgatc
     7081 gtttcgatgt catgatcgag aaagctaaaa aaaactatga acgtctagga ctgacagata
     7141 aagtgacttt gcttgaaggt caagcagcag atatcttgcc ggagttgtct ggtccgtatg
     7201 actttatttt tatggatagt gcaaaatcaa aatatattga atttttacct gaatgcttac
     7261 gtttgttaag aaaaggtggc gttttgatgg tagatgacat cttccaagga ggaacgatct
     7321 tgctcccaga tgaagagatt ccccgtggca aacgagctat ccatcgtaaa ttaaatgaat
     7381 ttcttcgtgt tgtgatggat catcccgatt tgacatcgac aatcttgcca ttaggtgatg
     7441 gtgtgatctt aatgacaaag gaatcagaaa caatcgagtt gtgatacatg attatggcag
     7501 cctgtagata atcgattgaa tcgagggctc aattcgaact tgatgtaaaa gtttctatat
     7561 ttgtaagtca attaaaggat acaggaaatc taaataaaca ggagtagcat gaagtcaatg
     7621 tagctcctgt tttcaattga taaccttaga acagctgaag gagaatgtct cgttttaaaa
     7681 gattgattta gtagttgggc caaaataatt cattatttgc taattaaata actatttcgt
     7741 attgacagaa gcaatttaat tatctataat tattgatgtt ccgctgattc ttgtcatagt
     7801 agctcagctg gatagagcat ccgccttcta agcggacggt cgggggttcg aatccctcct
     7861 gtgacgctaa aaaaccgtta gaaactgagg tttctaacgg tttttctttg ttttttgtgc
     7921 caatattgtt tgttttcaaa ggcaaataaa ataaaaaaac atccttatcg agtacaatta
     7981 taaggagcaa aaaaataaaa ctcatcagaa aagaggaaga agatatgacg atactatcta
     8041 gaattttagt gactctagtt gcaattgaat ttttttatat catgtatatc gaaacggtga
     8101 gaactgattc tgacacgaca agtagggttt ttaaaatgag taaagaagag ttaagcagaa
     8161 aatctgttca aacattgttt aagaaccaag gagtgtacaa tggactatta ggagtgggac
     8221 tactttatgg agcttatctt tcttccgcat ctaaagaaat cacaagtatg ctattaatat
     8281 ctattttttt tgttgcatta tatggcagtt tgactagcga tcgttggatt ttactcaaac
     8341 aaggtgggct agctctgttg gcattgcttt ctattttctt ataaatatag aaaaaataag
     8401 aatttatcta acaggaaact ttgtgaaaaa tattacttta tctcatgaaa tatgatattc
     8461 taatgaatgg tattttagaa aatttcatga aaaaggagtt agataggtgt tcaaaaatta
     8521 tgtttcctca ctaaaaaaag aattttcagg ttatgatctt tcaagatttc agaaagatct
     8581 tttggctggg atcacagtag ctgctgtagc attgccttta gccttggctt ttggggtatc
     8641 aagcggagct gatgcggccg caggtttgat tacggctatc attgccggta tcgttattgg
     8701 cggtttatca ggcggttttt atcaaatatc aggtccgaca ggtgcgatgg cagctatctt
     8761 gatgtctatt gcggcaacaa agggaatgca aggtgtattg cttgctactt ttttagcggg
     8821 tctattttta cttgcagcag gacttttccg attaggaaca ctgacttctt ttatcccagc
     8881 acccgtaatt acggggttta cttcaggtat tgctatcatt attgcattag gtcagatcga
     8941 taatttattt ggtgttcact cggaaggtac aaacgttatt gaaaaactaa gtagttatca
     9001 aacgatagga tttccgataa acacagcctc tttgatcatg ggagggctag tcattttagg
     9061 gatgtttatc tatccaaaaa aatgggcgaa acgaatgcct agttcgctgc tggctatcat
     9121 tttggcaaca gcagttatgc tattgttcca tttaccagta gcaactgtgg gtaagattcc
     9181 acaaacattg atcagtagta atcgtttgaa tatgggagat tttagctttt ctgccttgca
     9241 acaagtggca gttccagcta ttagcattgc tttattagga atgattgaaa gcttgctatg
     9301 tggtgcttcg gctggccgaa tggcaaataa gcccttagat agcaatcaag aattaatggc
     9361 gcaaggaatt ggaaatctgt tgcttccttt ctttggcggg attcctgcta ctgcggccat
     9421 cgcccgtaca agcgtggcca tcaaatctgg tgcacaaact agattagctg gtatgataca
     9481 tgcagttgtc ttactgctct ccatgcttgt ttttgcgcca gtgatgtcac agattccaat
     9541 gccagcactt gcaggtgttt tgattgtgac ggcttggcgt atgaatgaat gggaaacgat
     9601 caaggagcta tttactaata aatattggtc agcagttcta ttgtttatcc ttacgatgat
     9661 ttgtacagtt atctttgatt taagtattgc tatcgtgatc ggggtgatcg gcggctgtgt
     9721 attttttgtt gtcaaaagcg ctgctatcac gatttcatta gaaacgatcg attggcaaag
     9781 aatggggctg ccagaaacgg aaaagctgaa taactggaca gtagtgtata ttagtggtcc
     9841 actgtttttt atgtcttcag aaaaactcaa aaataaattg gaagaactaa atcatcacga
     9901 tggtgtcatt ttctccatgc gaggcgtacc aagtattgat ttgacggcac gtgcagtttt
     9961 tgatgaattt caagaaaaag cagagcaaag aggacaaacg attatctata cttccttgca
    10021 gccagaagta gaaaaacaac tttctcactt gtgggaacat acattcacgc atcaacatca
    10081 aacagttgct catgcgttga cagctttaca tgcgaaatat actgcaatta gccattaaaa
    10141 caatgaaaaa gaacagacaa gactgtaaac tgaagatttt atggtcttgt ctgttctttt
    10201 taatattgct gtaaaagtga agctagtact tgccagtctt gatggataac aggtacaagg
    10261 gagcgattgt agaaaatagc tgctggatga aaaagtggaa gaattcgata gtttttttct
    10321 gaccattcgt aggcatcctt ttgttctgtt aattgtaaga caggccctgt atagagttga
    10381 ccatgatttt tggtaaccgt atattctttg ccaagtaaac gttgaagacc aatgtttcca
    10441 agtgtcgcaa ttaattttgg ctggaccttc atcagttcgt aatccaatat tggtgcatgc
    10501 gcaaatactt cggaacgagt aggtgtccga tttggataaa cagtttctga ctgttgtgtc
    10561 cgtttattga tccgatgagt cacgcgatac gggcggctcc ttacagcact ggtgatatac
    10621 acctcttctc gtgttaagcc agttgaagaa agtgcctgca tcaattcttt tccagcttga
    10681 ccgctaaaag gaacatgcgt atcaatctct gttttccctg gggcttctcc cacgagcatc
    10741 agtttaggat gaagaggtcc ttgcccagcg ataaatcctt ctacaggacg gccgttcatt
    10801 cttaaagaag cttcttgtat cagttctttt gggtattcca tttatgctcc tcctttttct
    10861 tttattataa gaataaaaga aacacaatgc gactatttta tttggtagaa ctgtgaggat
    10921 agaaaagcag aagtattgct tgaatttttg ttttactttt tgattttaaa tttagttgac
    10981 taacggttgc tgttctggta agctagtact gttgtaattg tggactccac agctacaacc
    11041 gctcagaaca agtaataagc acagcatatg ctgtcacttc ggatggcgag tctaagtata
    11101 tcagaaggag gtgctttata gcatgtacgc aattattaaa actggtggta aacaagtaaa
    11161 agttgaagtg ggacaagcaa tctatgttga aaaacttgac gttgaagcag gagaaaaagt
    11221 tgtttttgac gaagttatct tagtaggcgg agaatctaca aaagtaggcg ctccaactgt
    11281 tgcaggtgca actgttgaag gtactgttga aaaacacggc aaacaaaaga aagtcgttac
    11341 tttcaaatac aaacctaaaa aacattcaca ccgtaaacaa ggtcatcgtc aaccatatac
    11401 aaaagtggtt atcgacgcga tcaacgctta atagcgtttg agaaagaagg cctatatatg
    11461 atcaaaggaa catttaagcg aaatgacgca ggtcagatcg tttcattcac tttgaccggg
    11521 catgctgatg ctggtcctta tggaagtgat atcgtttgtg cgggtgtttc tgcgttagcg
    11581 atcagcactg tcaacgggat tgcctcatta gccggttttg agccgattgt tgaaatgaat
    11641 gaagaagaag gcggttatct ttataccgaa gtcacttcag gaatgacaca agaacaaaac
    11701 aatattgctg agattctatt agaaaatctt ttattaggat tacaatcgat agaagctgaa
    11761 aactcagaat atatccaaat taaaacaatt aatgataaat aggaggtgca gaccatgttg
    11821 ttatctatga atctacaatt atttgcccac aaaaaaggtg gcggttctac atctaacggc
    11881 cgtgactcag aatcaaaacg tctaggtgct aaacgtgctg atggacaaac agttacaggt
    11941 ggatcaattc tttaccgtca acgcggtaca aaaatctatc caggtgtaaa cgtgggtatc
    12001 ggtggagacg acactttatt tgcaaaagtt gatggcgtag tacgtttcga acgtaaaggc
    12061 cgtaacaaaa aacaagtgtc tgtttatcca gtagctcaag aagcttaatt gaatcttatg
    12121 attaaagagg ttgtgacaag acttttgtca cagccttttt tgtttataaa aataaaagtt
    12181 gtagtggagg ctacgaagtt tactttgtct ttttacactt aatatttttt tgccaatttg
    12241 gaatcaggat gttttgataa ctatcgtttt caattgatgg aagctatatt gtttgctatc
    12301 agtgaaataa ttatatggta aacagtgttc tttttcttgt ttccattata aaaatcgtat
    12361 cgggattttt cgttctttta aattgaacat ttctatctat ccgcgtgtta tcaacaaaac
    12421 aaaatcaggt aaatagttga cgaaacagaa ataaatacat agtattatgt tttgaagcct
    12481 aagtacaata gtctatagaa aaggagcgat accattggca aaacgcattg accgaattta
    12541 tatgtatgtc aaagaaaaaa ccgctcattt aacaaaggca gaatatgatc aaggagtaac
    12601 aacacaggaa attgctgaag tgttagggat tcagcgaaca aatagcagta aagatctgaa
    12661 tcagctagtg cgcgaaggaa aactgataaa gacagacgga cgaccagtac gttacatata
    12721 tcaaacactc gttacgcacg taaagccagt tacaaaacat gtagtcagtt acaaagaaac
    12781 gccggtctct gaagaaaaaa atacactcca aatagatact aaggatatct ttgcaaaaat
    12841 cattggagct aacggcagca tgaaaaattc agtggaacag gcaaaagctg cgattttgta
    12901 tccgccaaga ggattaaatt gtttgattac aggacctacc ggatcaggaa agacttattt
    12961 tgcacacgcg atgtatcatt ttgcaaaagc gcatcatgta atagatgagg aaacggaact
    13021 gattgttttc aattgtgctg attatgccaa taaccctgag cttttgatga gtcatttatt
    13081 tgggtacgca aaaggtgctt ttacaggcgc agatgaagaa aaaatgggga ttatcgatca
    13141 agcggatggc ggtatgctgt ttttggatga gattcaccgg ttgccgcctg aaggtcaaga
    13201 aatgatcttt tactttatgg atcatggaac ttacagtcgt cttggggaaa caacgaaatc
    13261 ccatgaagca aatgtacgga tcgtaggtgc gaccacagaa gacccaggtt cttctctatt
    13321 agaaactttt gttcggcgta tcccaatcaa tatcaaactt ccggcttttg aaaaacgtcc
    13381 tgcaaatgag aaagtcgatt tagtaaaaat catgattgcc cacgaagcaa accgcacgca
    13441 gcgaaagatt tctttgacag aagatgtagc aaaagcattg atcggcagtg tgacctatgg
    13501 aaatatagga caattgaagt caaatatcca gttagtatgt gcacgaggat ttctaaatca
    13561 tatgcaatca cccgaaatct cgatcacaat cgatgatttg acagaaggga ttcgtagcgg
    13621 attgattcaa ttagcaagca accgcagtgc aatggcagag ctttctaaaa tattggagcc
    13681 aaagataact gtagcaccaa atgatacgat catgaaaatc caatcggact cctatgagtt
    13741 accttataat ctatatgata tcatcggaga taaagcagct ttactgaagt cggatggttt
    13801 ggatcaagaa gcaatcaatc atttcatttc tacagatatc aacatccatc tgaagtcttt
    13861 ctataaagac catggttttt cttttaatgc agacagcaaa ttagcagaat ttgtagatcc
    13921 gaaaatcata gaagtgacca atcagattta tgtaatggtc aaaagatctt taccatatga
    13981 gtttcagcaa aactttattt atgctatgag cttgcatatc agttcttttt tgaaaaaaat
    14041 caacttagga gaagaacggc atacgaatga taacatccgt gagatggctt ttgattttcc
    14101 agatgaatat gctgttgcga aagaagtgcg tagttatatc gaaacgtatt ttcaagtgga
    14161 gattccagaa agtgaagact attacttgac cgttctactc gtctcgttac gggcaaatca
    14221 agcaagcggc agaatcggtg tagtcgttgc agctcacgga aacagcacag caagcagtat
    14281 ggtccaagtt gtccaacagt tgttagatgc agagaacgta cgagcagtag acatgccttt
    14341 ggatatggat ccgaaaacag cattggaccg aattgaacgc aatgtacaag aagtagatga
    14401 aggaagcggg gtcatactct tagtagatat ggggtccctt gcgtcgttta attcgcaaat
    14461 tcaaagagac actggaattc cagtacgtac tgttgatatg gtaacaactt ctctagtttt
    14521 ggaaactgta cgaaaggtct cagtattagg gacagatcta gatatgttgc atgaatcatt
    14581 gaaaaatttt cgaggttacg cagaaatatc ttctgtagaa tctgttcagg aagcaaaacc
    14641 tattcataag aaaaaagctg ttttggctat ctgcgcatct ggcgaaggta cagcacaaag
    14701 aatcaaagaa ttgatcgaac gagcggtaaa caagcgtcaa gaaacagaat tagaagttct
    14761 agctttgtcg attgtggaag tgaaagaaga actgccagtt atccaaaaag aatatcagat
    14821 catagctact actgggatta ctgatttgaa tatccaagcc ccatttattc ctttagaacg
    14881 ttttattgat caaaatatcg aagtcatttt ggatcagttg ctgatggaat cagaattgga
    14941 ggaaactgaa ttcatctccc tagatgaaga gtcagctaaa aatacgtgtg ttgagtttat
    15001 ctctgataac ttcatcttta ttaatggtag taagttaatc gatccaatgt ggcaattttc
    15061 tacacaaatc tctcagacga cgggaatcgg cgatgaagaa tatggattca aaatcaacct
    15121 agtgatgcac acagcaggaa tgatcgaacg tattattcgg aatgaaccat tgactgtgga
    15181 agaaaacgaa ttgacaaata caacgaatga tcctctttat agccaattgg cagcctcagt
    15241 tgttctgttg gaagatcaga taaaagtaaa agtgccgata gaagaaatgt attatctgct
    15301 acgtctagtc cataatcagt tagataaaaa agaatacact gtgccatgaa tacacaaaaa
    15361 cgatacactt ttatagtgta tcgtttttgt gtattggtta agaatccttt aaattcaatt
    15421 agtgtatcgt aaaaaagttg gcacgctttt tgcttatagt agtgtgtgct aggaggtggt
    15481 tgttcacaca gaaatgatag gggtttctgt gaagtagtga ggaagaagga gttttgcaga
    15541 tggatattcg attggcacgt atagatgatc gtttaatcca cgggcaagtc gcaacagttt
    15601 ggtcgaagca aacaggtgtt gaacgtatca ttgtgatcag cgatgtagtc gctcaagata
    15661 aattgagaaa gtttctacta aaagaagctg caccgccggg gatcacagca aatgtaataa
    15721 ccgtgcagaa aatgattcaa atctatcata gtggattatt atcacgagca aaagtgatgc
    15781 ttttattcac caatccccaa gatgttgaag cggttgtccg ctgcggtgta cagttgaagt
    15841 cgttgaatat cggcggtatg agttttactg aaggcaaatc aatgattacc aattttgttt
    15901 cagtaaatca acaagatatt gattcctttc gttacttgaa cagtcaagaa gtggagttag
    15961 agattcgaaa agtacctgct gacaggaaaa tccttttgat ggaactacta gcgaaaatca
    16021 aaaaggtgta gcaagaatgg ttgttggaaa aggtcaaata attctgttgt aatggttagc
    16081 aattattaat atttttgaat ttgttacaac aaaagtaaat aggaggtata aatatggtag
    16141 ggattatcct agcaagtcat ggacaatttg ctgaaggaat cttacaatcc ggttcaatga
    16201 tctttggcga acaagaaaac gtcaaagccg tgattttgaa accaagtgaa ggtccagatg
    16261 acttgagagc caaattggaa gaagcggttg cttcttttga taatcaagac gaagtgttat
    16321 tcttagttga tttatggggc ggaacaccgt tcaatcaatc aaacacatta ttcgaagagc
    16381 acaaagataa atgggcaatc gtcagcggtt tgaatttacc aatgttgatt gaagcctacg
    16441 catcacgctt ttcaatggaa tctgctcatg aaattgcagc acacatcatt gaaacagcaa
    16501 aagacggtgt aaaagtaaaa ccggaagaat tagaaccggc agaagcaccg aaagcagcag
    16561 ttgaagacgc acaacctaaa ggagcacttc cagaaggaac tgtggtcgga gacggaaaaa
    16621 tcaaatatgt attagcacgt gttgactcac gtctgttaca tggacaagtt gcgacagctt
    16681 ggacaaaagc agtgcaacca aaccgtatta tcgttgtttc agatgcagta tcaaaggatg
    16741 atctgcgtaa gagactaatc gaacaagctg ctcctccagg tgtaaaagca aatgttattc
    16801 ctattagcaa aatgatcgaa gtagcaaaag accctcgttt tggaaatacc aaagcattgc
    16861 ttttatttga aaatcctgaa gacgtattga cagctgttga aggtggcgta gacatcaaag
    16921 aattaaatgt tgggtcaatg gcgcattcgg tcggtaaagt cgttgtaagt aaagtattgt
    16981 ctatgggaca agaagacgtt gaagcgtttg agaagcttga acaaaaaggt gtgaaattcg
    17041 atgtacgtaa agtacctaat gattcacgcg ataacatgga tgatattttg aaaaaagcaa
    17101 aagctgaatt agcaaaagcg taataaataa tattaaaatt tataggaggc ttatcatgtc
    17161 tattatttca ataattttag tcgtattagt tgcctttcta gctggtatgg aaggtattct
    17221 tgatgaattt caatttcatc aaccactagt ggcatgtaca ttgatcggtt tagttaccgg
    17281 taacctcgaa gctggtatcg tacttggcgg tacgcttcag atgatcgctt taggttgggc
    17341 aaatatcggt gccgctgtag caccagatgc tgcattagca tcagtagcat cagcaattat
    17401 tttagtttta ggtggacaag gtgttaaagg tgttccttct gcgatcgcga ttgccgttcc
    17461 tttggctgtt gcagggttgt tcttaacaat gatcgtacgt acaatcgctg ttccaatcgt
    17521 tcatttgatg gatgcggctg ctgaagaagg aaacatccgt aaggttgaaa tgtggcacat
    17581 cattgctgta tgtttacaag gggttcgtat cgcggtccca gcagcagcgt tgctatttat
    17641 cccagcacaa actgtgcaat cattccttga atcaatgcca gcatggttaa cagatggtat
    17701 ggctatcggt gggggaatgg tcgtagctgt aggttacgct ttggtaatca acatgatggc
    17761 tacaaaagaa gtatggccat tcttcgttat tggtttcgtt gtagctgcaa tttctcaatt
    17821 aacattgatt gctctaggtg ctttaggtgt tgcattagct cttatttact tgaacctttc
    17881 taaaatgggt ggttcatcaa ataatggagg cggaagcaac tctggtgacc ctttaggcga
    17941 cattttgaat gactattaat cttgaaggag gaaaataaaa tggcagaaga aaaaatcaaa
    18001 ttatcaaaaa aagatcgttt agctgttgcg tggcgctcta ccttcattca aggttcatgg
    18061 aactacgaac gtatgcaaaa cggtggctgg gcattttcta tgattccagc aatcaaaaaa
    18121 ttatacaaaa caaaagaaga acgttctgca gcattaaaac gtcacttgga attctttaat
    18181 acacacccat acattgcttc tccaatttta ggggttacat tggctctgga agaagaacgt
    18241 gcgaatggcg cgcctgttga tgatgtagcg attcaagggg ttaaagtagg tatgatgggt
    18301 cctttagctg gtgttggtga cccagtgttc tggtttacag tacgtccaat gctaggtgca
    18361 ctaggtgctt cattagctat gggaggaaat atccttggac caatcatctt cttcttaggt
    18421 tggaacatta ttcgttgggc attcatgtgg tatacacaag aatttggcta caaagctggt
    18481 tctaaaatta ctgatgacct ttctggcgga ttgttacaag acgtaacaaa aggtgcttca
    18541 attttaggga tgttcgtatt agctgccctt gtagaaagat gggtatcaat caaattctta
    18601 ccagttgtat caactgtaaa attagataaa ggtgcttaca tcgaatggga taaattgcct
    18661 tcaggtggtg aaggaatcaa gagtgcattt gaacaagtta ataatggctt agccctttct
    18721 cctgaaaagg taacaacatt acaagacaac ttagaccaat tgattcctgg tttagctgca
    18781 ttaggattaa cattgttatg tatgtggttg cttaagaaaa aggtttctcc aatcgttatc
    18841 atccttggtt tattcgtagt cggtgttgtt ggacacgtca tcggactttt ataatccata
    18901 aaaatatagc tcgggcgttg tcccgagctt tttttatata gtatactgtt cataaacaaa
    18961 tatctggagg gagaaaatgg ttcaatcaat caatacaaaa gtagacttgg taattgatgc
    19021 tacttcgttt acgggtctga cagattacgg gcaaatcatg ataggtgata atggttttga
    19081 gttttaccat gcgcgagata gacgcaaata tatccaaatt ccatgggaag aagtggatta
    19141 tgtactagca tcagtcatgc taaagggaaa atggattcct cgatatgcca ttcaaacgaa
    19201 aaaaaatggt acatacacat tttcttccaa agaacctaaa aaagtgcttc gtgcgatcag
    19261 agaatatgta gatgaagata gaatggtaca atcattgagt ttctttgatg tagtgaaacg
    19321 tggtattcgt agtatgttta aacgcaagta aaaaataaag aaaacttcta agaggaggtt
    19381 ttctttattt ttttactttg cagaagaaaa tatgaaatag caaaaccaat tatttgtccc
    19441 ctttaaatcc catatgctaa aattgattag caaagaagga ggttatatta tgagttggtt
    19501 atggacattg attgttggag ccgtgatcgg agcaattgca ggtgcgatca ccagtaaagg
    19561 aaattcgatg ggctgtgtaa cgaatatcat tgctggacta gtcggttcaa gtattggaca
    19621 agcgattttc ggcgaatggg gaccaaaagc tgcaggtatg gcacttattc catctatttt
    19681 aggcgcagtg attttggtag cagttgtctc atttttcttt ggaaaacgat cataagtttt
    19741 tacaaaatag taaactaaaa ggagttgcag cgagactgtg actccttttt ggctttattt
    19801 atttaaaata aaaagaagtt ttccggaaat ttttatttaa ctgtcgtgta cgaagaaaag
    19861 tttgctataa tggaaaaaag cgtgcaaaga aagagggagt ttcattatga tgctacgtgt
    19921 agaaaaattg cgcaaaaaaa tgcaagaaga aaatttggat tcatttttgg ttaccagtcc
    19981 gtacaatctt cgttacttaa cgaactttac aggtacgaca ggattagctg ttattacttt
    20041 ggaaaaagca ttttttatta ctgattttcg ttataccgag caggcggcag cgcaggctca
    20101 agggtttgaa attattaaaa atgtcggacc tatttttgat gaggtagcaa acttagtacg
    20161 caaagaagaa ctgcactcat tagcttttga ggaaacaaca gtctcatttt tagaatattc
    20221 ggttttagaa gaaatcatcg atgcggaatt agtacctgtt tcacagatga tcgaagaact
    20281 cagagaagtg aaagatgaag aagaaatcgc tattatcgaa aaagcatgtc atattgcgga
    20341 tatggcatat gatcacatcc tgaaaatgat ccaaccgggc atgactgaga ttgaagtcgc
    20401 aaatcaacta gacttttata tgcgttctct tggcgcttca ggtgtttctt ttgatacgat
    20461 cgttgctagc ggattgcgtt cggctatgcc gcatggtgta gcaagcaaaa aaatcatcga
    20521 acaaggtgac ttgatcacat tggactttgg ttgctattac gaagggtacg tttccgatat
    20581 gacacgtaca ttcgctattg gtgatcctgg ggaaaaatta aaagaaattt atcaaatagt
    20641 cttagatgct caattagcag ttattgatgc tgcaaaacca ggaatgactg gtgtccaatt
    20701 agatgcagtt gctagagatt atatttcaaa acatggttac ggggaagcat ttggacattc
    20761 tacaggacat gggatcggtt tagagatcca cgaaggacca aacgtttctt ctcgtgcaga
    20821 aaagcaattt gtaccaggaa atgtgatcac tgatgaacca gggatctact taccaggaat
    20881 cggtggagta cgtattgagg atgacttgtt gatcacagaa gatggaaatc gtgtgttgac
    20941 tcgttcacca aaagaattga tcatcttata gtttcatcaa tagaagaagt aagcgtaaaa
    21001 atcagtgttc acagactttg ttatagaaaa tcactcgatt taatgataca ctatagaaga
    21061 acttttaagc tgtaagagat aataaagttg ggtgttttct ccaattcata gcagtttgca
    21121 gctgatcggc ggcagttttc ttaccacgtg atcactatgc cgcatcatag agaattcgga
    21181 tgtatctcgt gcttaggcaa agggaggaat aaataaatgg cagatgaaaa aaatttgatt
    21241 ttaaacgcaa accaagattt aggagagatc gtgatcgctc ctgaagtcat cgaagtaatt
    21301 atcgggattg cagcttctaa agtagaaggc gtatacggaa tgcgcggaac ttttgccagc
    21361 aatgtcactg aattattagg acgtgcagct cacggaaaag gtgtttactt agcaaacgag
    21421 gaagatggct tgaaagtaga tctttactgt tatctagaat atggtgtttc agttcctaaa
    21481 gtagcaatgg aaatgcagga acgggtcagc cagcaagtcc ttttcatgac ggatatcgat
    21541 ttatctgaag tcaatatcca tgtagtagct gttgttcctg aaaaattaga acaaccaaca
    21601 atcgaagaac tttttgaaga cgaagaggaa gagaatgaat aaagaattat caagacacga
    21661 gattcgggaa atggcattgc aggctttatt tccattagac ttcaatgcag acctaacaaa
    21721 agaagatgcg attttcaacg caatcgaatt agaccaccgc gacatgatca acgaagatga
    21781 atcagagttt gttcctgttt atcttgatac gctagttggc ggtgtctgtg caaaaaaaga
    21841 tgaattggat aaagtgattg aaaaacattt aaaaaacaat tggcacatca atcggatctc
    21901 aaaaatggat ttggtgatct tgcgtatcgc gatttttgaa atgacttatg tgacagatgt
    21961 accagcggct gttgctttaa atgaagcaat cgagttggca aaaacattta gtgatgaccg
    22021 ttcacgtaaa ttcgtcaacg gtgttttatc caatgtattg aaagaattag aagctggggc
    22081 gtaagtccta gcttttttct atgaatcatc ataaacctgt tataaatcgg gaaaattcta
    22141 tggtagaatg aaaacacaaa aaatatctgg gagtggttag atggcagaat tgattaacgg
    22201 aaaagaatta gctgaaaaaa tgcaggcaga gattgctgaa aaaatccaaa aattgaaaga
    22261 taatggtatc catcccggat tagtcgtttt attggtaggg gaaaatccag ccagccaaat
    22321 ctatgtaaga aataaagaac gtgtagcaaa agaaatcgga atccattcat tggtagagcg
    22381 ttatcctgag acgatttctg aagacgaact acttgcagaa attgaaaaat acaatcaaga
    22441 tcctagtttt catggtatat tggtccaatt accattgcca aagcatatag atgaagaaaa
    22501 gatcctgtta gctatccgcc ctgaaaaaga cgtagacggt tttcatccga tgaatttagg
    22561 cagactcctt gcgggtaaac cggacaagat tccttgtact ccatacggca tcatgaaaat
    22621 gtttgaagct tattctatcg atccggcggg taaacgagca gtagttatcg gacgaagcaa
    22681 tatcgtcggt aaaccgatgg ctcagctact cttgatggca gatgcaactg ttactgttgc
    22741 tcattctaaa accgaaaatc tggctgaact ggcacaagaa gcagatattt tagtcgtagc
    22801 aataggacgt ggacattttg tcacaaaaga attcgtaaaa ccaggtgctg tagtgataga
    22861 tgtagggatg aatcgtgatg ctaacggtaa gctgatcggt gatgtgaaat ttgatgaagt
    22921 ggagcctatt gcaggctgga tcactcctgt gccaaagggt gtagggccaa tgactatcac
    22981 gatgctgatg tatcaaaccg taaaatctgc cgaagctttt ggagcaggtg aatagatggc
    23041 acaagaatat ttaactgtca ctacattaac gaagtatttg aaaagaaaat ttgatgccga
    23101 tccttattta gaacgcgtat atttaacagg cgaaatatcg aatttccgtt tacgtgccaa
    23161 tgcccatcaa tatttcagct taaaagatga ccatgcaaaa atatctgcta ttatgttcaa
    23221 aggagcattc caaaaattac gattccagcc aaaagaagga atgaaagtcc ttgtcgttgg
    23281 gcgtatttca ttatatgaag cgggaggttc ttatcaaatc tatgtagagc acatggagcc
    23341 tgatggaata ggtgctttat atcaagaatt ggaagaacga aaagaaaagc tgatgaagga
    23401 aggccttttt caagggccta aaaaaatgct gccacgtttc ccaaagcgaa tcgcggtcct
    23461 taccagtcca agcggagcgg ttatccgcga tatcatcacg acggtgaaaa gaagatatcc
    23521 aattgtccaa ttggttttgt tcccaacagt tgtacaagga aaccaagcag cggacaatgt
    23581 tgtcaggaat atccagagag tagaagagat gggcaatttc gatacaatga tcatcggtcg
    23641 tgggggtggt tcgattgagg atttatggcc gtttaatgaa gagcgggttg ctcgggcgat
    23701 ttatcaagct aatacgccgg tgatttcatc tgtaggacat gaaacagata ccacgatcgc
    23761 tgatttagta gcagatgttc gggctgccac tccaacagca gctgcagaat tagctgttcc
    23821 tgttttgtca gaagagttga tgcgcattca agaacggcaa gctagattgc agcaggcgtt
    23881 tgtacgccag atccagcgca aacaagaacg gttcgagcgt gctcgacgtt cctatgtatt
    23941 tcggcagcct gaaagattgt atgaaggtca gaccatcaaa ttagaccagt tgaaacagcg
    24001 attatatcaa gctacacagc aaatttatca tgcgaaagaa aaacaaacga tcgcattaag
    24061 ccaccaattg gaacaagtgg ctccgcttta tcgagttaaa gcagctcgcc aagaaactgc
    24121 ttatctagaa aaacgcttag aagagaaaat ccaacaatat atgaaacaac agcagcagcg
    24181 ttttgctcaa gctgttcaat ctcttgattt gttgagtcca ttgaaaatca tgggaagagg
    24241 ctacagctat acgacgttaa atgatcaagt agtaaaaagt gtcaccgaga ttcatcaagg
    24301 ggatttgctg acgatccatt atcaagatgg aatcgttaaa ggacaagtac aagagataga
    24361 caaaggagaa taaaaatatg gaaaatccaa cttttgaaga gtcactgcaa gaattagaaa
    24421 aaattgtcat gcagttagaa caaggagacg ttccattaga atcagctcta gattcgttta
    24481 aacgaggaat ggaattaagc aaacattgcc aagatacatt agctaaagca gaagagacct
    24541 tgacgaaaat gatgacagaa tcaaatgaag aagttgattt tgacggaaac gaggaaagct
    24601 gagatgagat taactgactt ttcagctatt catctacctg ctgtagaaaa agaaatcttg
    24661 tcttttttag atgagcatac aacggataga ggactctatg atgcgatgag ttattcagtg
    24721 aaagcaggcg ggaaaagaat acgtccctta ttacttttaa cggccgttgc ttcctttgat
    24781 gagcctattg atgttcccgt ctatcaagta gcagctgctt tagaaatggt gcatacgtat
    24841 tccttgatcc acgatgattt gcctgcgatg gacaatgatg atcttcgtag aggaaaacct
    24901 accaatcata aagtattcgg cgaagcatta gctatattgg ccggagatgg tttgcttaca
    24961 ggagcattcc agctaatcag tatggcacac ttaggtaatt ctcctaagct tctgctgctt
    25021 cagcaattag ctgtttgtgc gggatcacaa ggtatggttg ctgggcaagc tgcagatatc
    25081 gagggcgaat cgaaaaagct ttctttagaa gaattagcat ttattcatga aagaaagaca
    25141 gggcatttga ttcgttatgc cttgctggct ggtggcatat tagcgaagca acctgaagaa
    25201 atcttattgc tacttcaacg attagccgaa catttaggtt tagcttttca gataagagat
    25261 gatctactcg atgtgattgg tacaacgaaa acattaggga aaacagctgg taaagatgaa
    25321 cgtatggaaa aaaatactta cccgcgttta ttaggcctag aaaagacacg tgaggcgttg
    25381 gagattgagc tgttatccgc caacaagatc atagacaaat tagaagagga cgttttttct
    25441 tttgatggcg ggctgatgcg ccagatgatc aagcaatttg atgtggagaa atgagggaaa
    25501 gaatggaaaa agaacgcgta gatgtattag cggtcaaaca aggattattt gaaacaagag
    25561 aacaagccaa acgttcagtg atggctggtt tgatttacaa cgaaaaaaac gagcggtttg
    25621 ataaaccggg agaaaaaatt ccagtgagta gtgaattaag agtcaaaggt aaaaaacttc
    25681 cttatgtttc tcgaggggga ttaaaattag aaaaagcact caaagcattt gatctatctg
    25741 ttgaaggaaa aacattgtta gatattggtg catccactgg cggatttact gatgcagctt
    25801 tgcaaaatgg cgcaaaaatg agttatgctt tagacgttgg ctacaatcag ctagcttgga
    25861 aaataagaca agatcctaga gtcgtcgtaa tggaaagagt gaattttcgt tatgcaaaac
    25921 ccgaagactt taccttagga attccagaag ttgctgttat cgatgtttct tttatttcac
    25981 taaaactgat gctgcctcca ttacatgcta ttttaaaaga agagggagaa gtgattgctt
    26041 taatcaaacc ccaattcgaa gcaggcagag aagctgtagg gaaaaacgga attgttcgtg
    26101 accctcagac ccatcaaaaa gttttagaag acattttgtc atttgcggca caaggtggat
    26161 atgatgtgct ggaattaagt tattctccga tcacgggggg agaaggaaat atcgagttct
    26221 tggctcactt gaggaaagta cccgaaagcg gaacaatcaa ttcagctatt aatatggcag
    26281 aagtggtttc aaatgcacac gaacagtttg accataaata aattgtaact gtgaaaatca
    26341 agtgagaagt atgcggattc ttctttatta taccaaggaa ataagctatc attgattaag
    26401 aagaatatgc taaggagcga agacgtaatg agaaaaaaag atcggcatcg tttgattact
    26461 cgattgttga gtgagcaaga tatccgaaag caggaagaat ttgttgaaat cctgaaaagc
    26521 aaaggaatat ctgttactca agctacgatt tccagagata taaaggaact gaaactgatc
    26581 aaggtaccgg cttcagatgg cggctaccgt tatagcatac cagctgaaac aaacgaagat
    26641 gtaaatacta aattggaaaa actattaaaa gacgctttcg tggcagttga ccaaatggaa
    26701 aaatttgtta ttttgaaaac attgccggga aatgcttcgg cagcagcaaa tttgattgat
    26761 aaacgttaca agaaagaatt attttcgatt atcaatgatg acgataatgt tttgatgatt
    26821 acacgaactg aagaccaggc agttttattg aagaaagact tcctgcatta cctttaagtt
    26881 gaggagcgaa tgtgatgcta caagaaatca gtattactaa ctttgcgatt attccggaat
    26941 tgcggctttc ttttcacgaa ggaatgaccg cgctgaccgg agagactggt gcgggtaaat
    27001 ccattattat cgatgcttta ggtctgttag caggaggaag aggttcaagc gattatatac
    27061 gtcaaggagc agaaaaatgt gtgcttgaag ggttgtttga actaccaaaa caagaaggat
    27121 tctctgaact gatggttgaa ttagggatcg aaacagatga ggacaatctg attgttcgac
    27181 gggatatgtc cttaactgga aaaaatgttt gtcgagtgaa tggtcatatt atcacattag
    27241 ctaatctgag aagaatcggc agttatttag tagatatcca aggacaaaac gaacatcagg
    27301 aactgctcca gcctgaatca catcttgctt tattggatcg ttttggagat gtagctttcc
    27361 aacagaagaa aaagagttat cagcaagaat atatctctta tcgtgagctg gaaaaaaggg
    27421 tacgaaaagt gcagcaaaat gaaaaatcct atgttcagag aatagatatg ctccatttcc
    27481 agcaagaaga aatagctgct gctgatttgc aagtcggaga agaagagaaa ttaaaagaag
    27541 aaagagaaaa acttagcaat tatcaaaaaa ttgctgatgg attagctgca ggttacggag
    27601 cactgacgaa taatgaacaa aacagtgtag atggtgttgg cttggccgtt tcagaactgc
    27661 aagggatcgc ccatctagat gtggagtatg aagcaattta tgaaaatatc caaagtgctt
    27721 actatttgct gcaagatgca atcggcgata tgagtcgtca gattgattta ttggagttag
    27781 atgagagtcg tttagaagaa gttacgcaac gtttagaatt aatccgacag ttgaaacgta
    27841 aatacggcga atccattgag tcaatattgg cttactatga cgaaatcacc gaagagctag
    27901 cttcttctga tttttcagaa ggtcaattgg acaaaatgaa agaggaacta gagcaaaaag
    27961 agcttctact tcagcaacaa gccgcagatc ttcatgaagc gcgtaaaaaa atcgcaaaag
    28021 aattggaaaa atccattctg catgaattga aaagtctgta tatggaaaac actgagtttg
    28081 aggtacgttt cttaaaagaa gaaaatagac aattgaatca tgatggattt gaccaaatcg
    28141 aattttacat tacgaccaat ccaggagagc cactgaagcc tttggtgaaa gtcgcttctg
    28201 gtggcgaact gtctcggatg ttgcttgctt tgaaaactat tttttcttct gagcaaggtg
    28261 tgacaagtat tatttttgat gaagtggata caggtgtgag cggaagagtt gcacaggcca
    28321 tcgcagataa aatttctcaa atatcgaaat actctcaagt tttgtgcatc actcatctgc
    28381 ctcaagttgc tgcagtcgcc gactatcaat attatattgt gaaagcagtg attgatggac
    28441 gtacgcaaac ttctgtttca gagttgaaaa caaaagaaag agaagaagaa attgcacgta
    28501 tgctggctgg aagtgagatt acaaaactca cactcgaaca tgcaaaagaa ctattgaaac
    28561 tcgcaaaaag gacctgagcc acctcgcagt ggttcaggtc cttttcagca gctgatagct
    28621 atattttatt tttacatgac actcattaca gcagtcaata caacagatcc aatcgttaaa
    28681 atcagcattg tccaaatgac aaattttgtt actttagcaa aagtacttgt ttgttttttt
    28741 tcacgcattg ttttcaacct cgtcattaat tagttgattc aagtgtacaa tcttttttcc
    28801 atttccacaa gagggctttt ttataaaaac ttcccttttt ttaaataata ggtagttacg
    28861 atacaaataa ataatgttaa tagaactagc catataaagg catcatccct ttcagcaata
    28921 ggtaacttaa tgttcattcc atagatactg ccgacgattg tcggtatagt taaaataatc
    28981 gttaaagaag ttaaaatttt cataatatta ttcagattat tcgatacgat tgctgaaaaa
    29041 gtctgattca tttgttctac tagttttaat tgaatgcgag aagtagtcat tgcctgctct
    29101 gtttccacca atacatcacg taaatgattt ttcaaagcat gattttcttg gaaaagttta
    29161 ttttgcgcta gttctttcaa tgcatctaaa ttagcagaag tcgcttctct gaaataaacg
    29221 aggctttttt ggatatccat gatttgataa agttgtttgt tttcagtgga tacttgaagt
    29281 ttggcttcca aagtatcgca ttgagattga agcgtagcta aataactgtt gtaagagagt
    29341 gtgacctgcc aaatgaattg aaagaagata ttgagattag gcgaaatatc actttttgga
    29401 aaggtctggc taaagacttt ctttaaaaac agtggttcat gattcgtaat tgtgataagc
    29461 ttctttttgg gcgtcaaaat aatagagagc gggtatgtat ccacttgaaa ataaccgcta
    29521 ggacttgttt tagcaaaagg atattgtaac aataataacg ctggctttgt caactttgat
    29581 tgattgaatt cttctgtacg ggaattttct gcatcgtcca atatggaagt gatgtaatct
    29641 gacggaattt caaattgatg tgtcagctga tgaatctctt cttgattagg acgttctaca
    29701 caataccaga tcgtttggtc agtttccttt ccggtattac gcagtttctc attatctaat
    29761 gtaaaatatt tgatcatgat tttcctcctg ttgcctgttt cttttattat aacggatagg
    29821 gaattggaat acatggaatg tgtttgtaag atatagaata taactgcata aatttaaata
    29881 aaatgaaaca gttgtgtaaa gtatatacag tatacttgaa gcaacgataa aaaaggagtt
    29941 tacgttcatg ggcacattca caccacttat cttgttttgg tatatttacc cggtaatcgt
    30001 tttgtttggt tgccaatttc ttgtaaaatt attttcgtta aatcgtcgtt ttaaaataaa
    30061 agcccctgat ttggcgattc cttttttgtg gggcggtatg catgcattgt ctagaaatac
    30121 agggacaatt tctatcttac cgtttttctt tatttctgtt ttgctaatgg gaatttgtat
    30181 tgctgttttc caagcatatt attatgaaga aattatttat ccgcgttatt tcaagatgac
    30241 ttggcgtttg gtttttctgt tgacaatcgt cttgtacgct gtcttgatca ttgtgaacat
    30301 actttcttac ctatgaaaaa aatagaaaag ctgtaaaaag tcttttaaaa ggctttttgc
    30361 agcttgattt ttaaaaaaat gtttatgaat aaaaaaagtt gttcccggat ttcccagaaa
    30421 aggtggtaga aagtggggga ttgtggtaga ctaattttag ttagtggagg aatggggggc
    30481 tttcaatgtt gatgggtgaa ttccaacata atattgatgc aaaaggacgt ttaatcgtgc
    30541 cctcaaaact tcgtgaagaa cttggcgaaa agtttgttct gacacgcgga ttggatggct
    30601 gtcttttcgg ctatccgatg agcgaatggg aaaatctgga agctaaactg aacgaaatgc
    30661 cgttggctaa aaaagacgca cgtacttttg tgcgattctt ctattcggct gccactgaat
    30721 gcgaacttga taaacaagga cgaatcaata tacccagcac gttaagaaat tatgcagctt
    30781 tgacaaaagg ttgcgtagtc atcggtgtat ctaaccgaat tgaaatttgg gatgagacac
    30841 gatggcaaga tttttctgca gcagcagaag agaactttga cgaaattgct gaaagtatga
    30901 ttgatttcgg actataagaa taggaagagg ctgactaatg actgaaacat ttcaacacta
    30961 tacagtcatg ttgaaagaaa cggtagatgg attgaacatc aaaccggatg gcgtttatgt
    31021 agactgtaca ctcggcggtg ccggacatag tgaatattta ttaagccaac taagttctaa
    31081 agggcatctg tatgcgtttg accaagatca aaaagcaatc gatcacgcca agctgcgttt
    31141 agcttcttat gtcgaacaag gtatggtcac atttatcaaa gcgaacttca gagaattaga
    31201 agaacgcttg agtgagcatg tagataaggt agacggcatt ttgtatgatc taggggtttc
    31261 ctctcctcag ctagatgaag cagaacgtgg atttagttat catcaagatg caccgttgga
    31321 tatgcgcatg gatcagtcag ctcctttatc cgcttatcac gtaattaatg aatattccta
    31381 ccatgaatta gtaaagattt tcttccgcta tggcgaggag aaattttcca aacaaattgc
    31441 tcgtgaaatc gaacgagtac gaaaaacaaa accaatccaa acaactggtg agcttgtcga
    31501 aatcattaaa caagtcatcc cagctcccgc aagaagaaaa ggcgggcatc ctgcaaaaag
    31561 aatttttcaa gctgtgcgga ttgcagtaaa tgacgaactc ggtgccgaag aggcctcttt
    31621 agaacaggca atccggttat tgaaagtcaa tggaagaatc agcgtaatca cattccattc
    31681 attggaagac agaattgtca aaagtatgtt taaagaatat agtacggtac aagatctacc
    31741 accgggactt cctgtcatac ctgacgaatt ccaaccagaa ctgaaagtca ttactcgtaa
    31801 accaatcctt ccttcagagg aagagctaac agaaaacaat cgatcacgca gtgcaaagtt
    31861 acgtattgca gaaaaaataa gaatgaaaga ggagtagaat gatggcagaa ttaaagaaaa
    31921 tgcaagatta cgaatatgat ttacctgaat tggtggatca gccggaacaa caaatgcctg
    31981 attcttcttc gaatcaagaa atcattcttc cacaatcacc ggcaaaacga ttaaagcaga
    32041 tttctgcttt ggaaaaaatt gctgtggcaa gcgtggcgat tgctattgtt gctttatgtg
    32101 ttttaacagt tatgctgcgc acaaatatca gcggagctga aaaagaaatc acgtcaatac
    32161 aaacagaaat caatgataaa aagcaagaaa aaacgagttt agaacaagaa aaaaacgaac
    32221 tttctcgtac tgaacggatt aaacaaatcg cagaagaaaa aggattgtct attaatgacg
    32281 acaatttaag gaaagtgaag taaatgagtc ttaaaaataa aattcggcgg tttatggaaa
    32341 agaaaaatct aaatccgatg aacaatcgca aaaaagtagg tatcatttta tttgctacga
    32401 gtattggaat gttcttttta tttgccgtcc gtttctcgta tatcgttatt ggaggtcatg
    32461 ttgctggtac ctcattagct gaaaaaacaa aacagttgta ccaaggaagt gaagtagtaa
    32521 aagcaaaaag aggaacgatt tatgatcgta acggtgtggc gctcgcagaa gatgcctcct
    32581 cttattcaat taaagcgata ctctcaaaaa catatacctc tggggataaa aaactgtatg
    32641 ttgaagaaaa gaattttgat aaaatagcag aaatcctgca taagaatctt tctatagaca
    32701 aaaaagatgc actgaatatt ttagaggacg gcgcaaaaaa agagctgtat caagttgaat
    32761 ttggtagcta tgggaaaaat atctcccaag aaaccaagca aaacatcgaa gcggacatga
    32821 aaaaagaagg tgtcgctggt ctttattttg tcgaccatca ggctcggatg tatccaaatg
    32881 gcgtcttttc ttctcatttt attggatacg cagttccaga caaagatgag aatggcttag
    32941 ttggtaaact aggtttggag tctgcttaca atgatatctt gagcggtaaa gatggtaaga
    33001 ttatttatca aaaagataat tttcaaaatc ctctgccagg tacagtggca gaagaagaaa
    33061 aagctgtcga cggacaagat atctacacga ctttagacag cagactacaa agttatttag
    33121 aaacactgat ggatcaagta aatgaagaat atcagccaga agaattgaca gcagtcctga
    33181 tgaaagcaaa aaccggagaa atcttggcaa tgggacaacg acctactttc aatcctgaaa
    33241 caatggaagg attgactgga aaagatgcta tctggcgtaa tttcttggta caagatagtt
    33301 atgaaccagg atcaaccatg aaagttttca ctactgcagc tgctatcgaa gaaggagaat
    33361 tcaacgagaa tgaaaccttc caatctggaa aaatccaagt agaagatgct acgatcaatg
    33421 accatgactt tggtgaaaaa ggtgtgctga ccatgcgtca agctctttca tggtcaagta
    33481 acgtcgggat ggtcatactg gaacagcgat taggcggacg atggtataac tatttacaaa
    33541 aactgggatt tggacaaagt actcattctg gattagatga tgaagtaaac ggtgctttac
    33601 ctacattaaa tattgtcgac cgagcaatga gtgcttatgg acaagcggta ggtgtgacga
    33661 atttccaaat gatgaaagct ttcacttcta ttgcgaataa cggtacaatg attcaacctc
    33721 gatatatcag caaagtggtg gatccccaaa ctggagaaga gcgaacaact caaacagaag
    33781 ttctagggca acctttttca aaagaaacaa cagaaaaagt ccgtgaatat atgagagatg
    33841 ttgtcgaaag cgaaaactac ggtagtgctt atggtgtata tagtgtacct ggatacaatg
    33901 tatcggcaaa aaccggaaca gcccaaatcg cttctgatac agggggatat caaacaggag
    33961 atacagccta cctgtattcg attgtggaga tggttccttc tgaagatcca gactatgtct
    34021 tgtatctaac aatgaaacac cctaagacat atgatcgaat ggcattggct aaaatcgcaa
    34081 atccattgat gaaacgggca atggatttta aagaaactga agaagattca gatacagaaa
    34141 caaaaacaga aaaagtatcc gtagccgatt atcgtaatct tgaagcggac gttgcagcag
    34201 ctgatgctca aaaaagtgga ctgcaacctg tagtgatcgg taatggtaaa aaagtacaaa
    34261 aacaatctac tgccaacggt gatcagttga tctctggaga aaaactgatc ctatatactg
    34321 gtggtgataa gctgatgcct gacgtaacag gatggtcaaa agcagatatc atgaagctgg
    34381 ggaaaatcct cggtattgaa gttagttttg atggagatgg ttattgtgtc aaacaagaac
    34441 ttgctccata tgaaaaaatc actgaagaca aacttagttt tactttagaa gaataagaag
    34501 agaaatagga gagaaaagag catggaatgg acacaagcat tgattcccat tgtcagcagt
    34561 tgtgcaatga caattgccgc aatgccgcta tttattggtt attttcaaat gaaaaagcaa
    34621 ggtcaggcga ttcgtgagga gggaccaaag tggcacaata gcaaagccgg aactcctaca
    34681 atgggcggct tagtgtttct gatcggctct attttgacag gaatttgggt tggagcttgg
    34741 caaaaacagc taactcctac tcttttcatc ttgctttttg tattagcgtt atacggagta
    34801 atcggctttc ttgatgattt tataaaaata ttcaagaaaa gaaacatggg actgaactcg
    34861 aagcagaaat tgattggaca gattctaggg ggaatcatct tttatcttgt ttaccgttct
    34921 gaaggttatc caggtgtatt gaatttcttc ggactagatc tacctttagg aatcatttat
    34981 ggtattttcg ctctcttctg gcttgttgga ttttcaaatg cagtaaatct gacagatggc
    35041 atcgatggat tagttgctgg cttaggaagt atctcttttg cagcttacgg tctaattgct
    35101 tggcatcagc atcagtatga tgtattaatc atttgcttaa gtgttttagg aggtttactg
    35161 ggtttcttcg tctacaacag aaaacctgcc aagattttta tgggagatgt cggttcatta
    35221 gcgttgggcg gacttttagc ggcaatttct ataatgctaa accaagaatg gacattgctt
    35281 ttgattggtt tgatctatgt tatggaaaca gcaagtgtga tgcttcaagt tacttccttt
    35341 aagcttacag gaaaacgaat ttttaaaatg tctccaattc atcatcactt tgaaatgtgc
    35401 ggatggtcgg agtggaagat tgatacagtt ttttggttga tcagcattgt gacttcgttg
    35461 atcacattat ggatcgtttg gtaggagatg acgggaaatg aaaaaaatat caacatatga
    35521 aaataagaaa gtgcttgtct taggactcgc aaaaagcgga gtaagtgcgg ccaaattatt
    35581 acatgaattg ggcgcacttg tcaccgttaa tgatggaaaa ccttttgacg aaaatccgga
    35641 agcacaagag ctgctttctt tagggataaa agtaataaca ggtagtcatc caatcgaatt
    35701 gttggatgaa gaattttcat tgatggtcaa aaatccaggg attccctaca gtcatccttt
    35761 agtagcaaaa gcccaagaaa tgggtattcc ggtaattacg gaagttgagc tagcttacga
    35821 agtagcggaa tgcccaatta ttgggattac aggaacgaac ggaaaaacaa cgacaacaac
    35881 gatgacagga ctactactga atgcaggagc agatcaagga attgcacgtt tagcgggtaa
    35941 tattgggtat ccagcaagtg gtgtagcgca agaagcaaaa tcagaagata aaattgtgat
    36001 ggaattgtct agcttccaac tcatgggaat cacagatttc cgccctcata tagcggtgat
    36061 taccaatatc tatgaagcac atattgatta tcatggtaca agaaaagaat atgtcaaagc
    36121 taaatggaac cttcaaaaaa acatgacaga aaaagattat ctgatcctta actggaatca
    36181 gtcagaacta caagaattag cgcaaagaac aaaagcacgc gtactgcctt tctcgacaaa
    36241 agaagtgttg gaagacggtg tgtatgctga cgattactca atatactata aaaaagaaaa
    36301 aataatggaa atatctgaac ttggcgtacc aggtaagcat aatgtagaaa atgcactagc
    36361 tgctatttct gtagcaaaat tgtatggtat atcaaatgaa gctatacgag aaacgttgca
    36421 ttttttccat ggcgtaccac atcgtacgca atatgtagga gaaatccaag gcagaaaatt
    36481 ttataatgat tcaaaagcaa ccaatatttt agcgactaaa atggctctaa gtggatttga
    36541 aacgtctaag gtcgtgttat tggctggcgg attagacaga ggaaatacat ttgatgaatt
    36601 aattccttca ttaaaaggaa tcaaagcaat ggtcgtattt ggccaaacaa aagaaaagtt
    36661 gatggatgct ggaaaaaaag caggcatcga gacaatagta acagccgatt ctgtagaaca
    36721 agcagttcct ttagcattag aaaacagtac agatggtgat gtagttttat tatcaccagc
    36781 gaatgccagc tgggatcaat acccaaattt tgaaactaga ggaaacagat tcatggaagc
    36841 tgtgaaccga ttaaaataga aaaagtagaa gcgagcgata aaaaatgaaa attttggtta
    36901 caggcggagg aacaggggga catatttatc cagctcttgc ctttgtcaat tatgtaaaga
    36961 ctcaagaacc gaatgctgaa ttcatgtatg tgggagcaaa acgaggtcta gaaaacaaaa
    37021 tcgtccctga tacaggaatg cctttccata ccttggagat tcaaggattt aaacgaaaaa
    37081 tttccatgca taacatcaag acggtccgat tgtttcttaa gagtatccgt caagcaaaaa
    37141 aaatcctcag tgattttcaa ccagatatcg ttatcggtac tggaggatat gtttccggag
    37201 cggtagtgta cgctgcagcc aaaatgggta ttccaacagt tatccatgaa caaaacagtg
    37261 taccaggtgt taccaataaa tttttaagcc gatatgtgga tcggatcgct ctttcattta
    37321 aagatgcggc ttcctttttt cctgaaaaca aagcagttct tgtaggtaat cctcgagcac
    37381 aagaagtagc agatactaaa aaatcagata ttttgaaaac atttgatttg gatccagaaa
    37441 agaaaacagt cttgattttt ggcggtagtc aaggagcact taaaatcaat caagcagtat
    37501 cagttttttt aagtgaatat attccttctt cttaccaagt tttgtatgct tcaggagata
    37561 gatattatga agagatccta aataaaataa atcctgaaat acaaaatatt agtgtccagc
    37621 cttatatcaa aaatatggca gaggtcatgg cttccagtga tctgctggtg gggcgtgctg
    37681 gtgcaacttc gatcgcagaa ttcacagctc tggggttacc ggcagtgttg gttcccagcc
    37741 catatgttac gaatgatcat cagactaaaa atgcaatgag tcttgttcac gcaggagcag
    37801 caaaaatgat tgcagataat gaactaacag gtgaatcact aagtcagaca gtcaacgaaa
    37861 tcatgggtga tgaagaattg caaaagcaaa tgtgccgagc ttctaaagaa caaggaatcc
    37921 ctgatgcttc aaaacgtctt tatgacttag taaaacaaat aatcaaaaaa taaaggcggt
    37981 ggtcttagtg agtaagaaaa aagaaccaaa aaaattgtct catccgtctt ctgatgaaaa
    38041 caatttgact ccttggcaaa aggctaaccg aaaatatctg gcagaacaag agatgaaaaa
    38101 ccaagagcca aaaaaagaag acataacatt agatacaccc ctttcagatt ctgaagaaga
    38161 aaaaacggtt caagaggaac agcaagaaga aagccagtcc gagaaaaaaa tcgaattttt
    38221 tgaagagatc aagcaaaaga aaaacggtgg accatataac ggttcttttt tgaaccggct
    38281 gcccaattta aaaagtcaac ggcacaaagt actatttcgt cggttagctt tgatcatcac
    38341 tgtattgggt ataccgctga tttttttgat ctattatgtt tctccttata gcaaattaca
    38401 agcagttgca gtatcaggca ataagacagt caacagccaa gaaatcatct ctgatacaaa
    38461 attgtcatta ggagaaaatg tttggggaca gtattttcat cgatctactt atatcgatcg
    38521 ccttaaaaaa gcacagccaa gaatcgaaac agcaaatata catttcaaag ggatgaatga
    38581 attcgatttg gatgtaacag aatataaaga gattgctttg atcgcaaaaa acaatcaata
    38641 ctacccagtg attgaaaatg gaacagtatt agatgaaaaa gtagcaaatc ctacaaaaaa
    38701 tctccctatc ttggaagagt tcaaagactc tgcaaaaatc aaagaattaa caaagcaata
    38761 caatcagtta tcatcagaat tacaaaaagc aatttctgaa atcaagtaca cgccaagagc
    38821 aagcaataaa aatctgatcc aattgaatat gaatgacggc aatcaagtta ttgtaaatat
    38881 caataatctt gcgaaccaaa tgaaatatta ttcgcaagta gccaaagata tggatgaaaa
    38941 aggtgtgatc gacatggaag ttggtatctt ctcgcatcct tacggtagca ctcaagaaac
    39001 ggaagaatcg ggccaatcgg aagaaacaac tgaaagtgaa gaaaatgcgg atcaaacaaa
    39061 cgaatcaagt gaagaggcag aaacgcaaaa agcacaaaca cctggatcgt ctagcacaaa
    39121 cggatcatct gattcaaatg attcaagtga aacaagtcaa atagaagata atccaagtac
    39181 aactccgtca agtttaaatg aaaattgata aaacaacttt aaaaagtaag aatgtacttt
    39241 ctaaaaacca gtaatttatg gtagaattgg aagcagtgaa tatatctatt aataagtata
    39301 ttttaaaacg gacgctagtt gttcgcttgt atatggaata gtaagtagga gggaatccca
    39361 ttcatggcaa aaacaggaat gtatgtaggc cttgatattg gaacgacatc tgttaaagtt
    39421 gtcgtggctg aatatatcga cagccaaatg aatattattg gagtaggaaa cgcaaaatca
    39481 gaagggatta accgaggcat tatcgttgat atcgataaaa cggtccaagc gatacaacga
    39541 gcagtacgac aagcagaaga aaaagcaggt atccagatca aaggcgtaag tgtcggtttg
    39601 ccagcaaaca tgcttgaagt agaaaattgc cagggaatga ttgcagttaa cggagattca
    39661 aaggaaatca ccgatgaaga tgtcagaaat gtggcttcag ctgcccttgt tcgttcaata
    39721 cctcctgaac gtcaaatcgt gtcaatattg ccacaagatt ttactgtcga tgggttcgag
    39781 ggaatcaaag atcctcgtgg aatgatcggc gtaagattag aaatgtacgg tctgttgttt
    39841 acaggaccta aaacgatcat ccataacata cgtaaatgcg tggaaaatgc cggattaatc
    39901 gtcaacgaaa tggtgattac tcctttagcg ctgactgaat caattctttc tgacggagaa
    39961 aaagattttg gtacgatcgt cattgatatg ggtggcggcc aaacaacaac ggctgttatg
    40021 catgataagc aattgaagtt taccaatctt gaccaagaag gtggcgaatt tgtcactaaa
    40081 gatatctcta ttgtattaaa tacgtcattt aacaatgcag aagcgttgaa aataaactac
    40141 ggggatgcat acccagaacg gacatcacca gatgaagaat ttccagtaga tgtgattggt
    40201 cagtcagaac cagtgaaagt cgatgaacgc tacttgtcag aaattatttc agctcgaatg
    40261 gaacagattt tcaacaaagc aaaagatgca ttggaccaaa tcgaagcatt agagttgcca
    40321 ggtggcgtcg tattgacagg cggagctgct agtcttcctg gtgttgtgga cttggcacaa
    40381 gaaatcttcg gcgtaaatgt aaaattgtat gttccaaatc acatggggct gagaaacccg
    40441 gtatttacaa atgtaataag tatcgtggat tattcagcaa acttgagtga agtgtatcaa
    40501 ttggcaaaaa ttgctgttac gggtgaaacc tcagcagctc gtcaaatggt agttgaacaa
    40561 gaaacaacaa acacatatga gagctatgaa gcacctgaag aaacaatcta cgatgagccg
    40621 gaaccaaaag agtccggaga aaatgtaaaa aataaaatca agggtttctt tacaaatatt
    40681 tttgattaat tcatcctggg aggaataaga tatggaattc tcaattgata ataatatcaa
    40741 tgacggcgca gtaatcaaag tcatcggagt cggtggtggc ggtggaaatg ctgtaaaccg
    40801 aatgattgaa gaaaacgtta aaggtgttga attcattaca gccaacacag acgtgcaagc
    40861 attaaaaaat tcaaaagctg aaacagttat ccaattaggc cccaaatata ctcgtggttt
    40921 aggtgctggt tctcagccag aagttggaca aaaagcggca gaagaaagtg aacaatcctt
    40981 acgcgaagct ttagacggtg cagatatgat tttcatcact gcaggaatgg gtggaggaac
    41041 cggtacaggt gctgcaccaa tcgttgcagg tatcgcgaaa gaattgggcg cattgactgt
    41101 tggtgtcgtg acacgtccat tcacttttga aggaccgaaa cgtggccgtt ttgctgcaga
    41161 aggtatcgct cgtttgaaag aaaatgtgga tacattatta attatctcaa ataaccgttt
    41221 attagaagtt gtagataaaa agacaccgat gcttgaagct ttccgtgaag cagataatgt
    41281 tttacgacaa ggtgtacaag gtatctctga tttgatcaca gcgccaggtt acgttaacct
    41341 tgactttgct gacgtgaaaa cagtgatgga aaaccaaggt acagcattga tggggatcgg
    41401 tgtggctagt ggagaagatc gcgtagtaga agcaacgaaa aaagcaatct cctctccatt
    41461 gcttgaaaca tctattgatg gagctgaaca agtacttctt aacatcacag gcggtttgga
    41521 tatgacattg ttcgaagcac aagatgcttc agatatcgta gcaaatgcag caactggcga
    41581 tgtaaatatc attctaggta catctatcaa tgaagaaatg ggcgatgaaa tccgtgtgac
    41641 ggtcatcgca acaggtatcg atgaatcaaa aaaggaacgc aagtctagcc gaccagctag
    41701 acaagcacaa atgcaatcac ctgctcaaaa gactgtatta gatatggatc aagctaaacc
    41761 aacttcttca gaagaagaaa acagttttgg cgattgggat atcagacgtg aacaaaatgt
    41821 tcgtcctaga gttgatgatt ccaattttga caatatcgaa aaaaaagaat ttgatacatt
    41881 caaccgcgaa gaaacaaaat ctcatggcga tgatgaatta aatacgccac cgtttttccg
    41941 tcgtaaaaaa taaaggagag gaagagcaca tgattgctga caacttgcag aaaatcaagc
    42001 aagaaattca ggattcgtgt gctcttgttc atcgtgatcc gaaagaagtt acattagtag
    42061 ctgtaacaaa atctgttgaa tccgctcaag ctgaagaatt ggttagagct ggcgttatga
    42121 attgtgcaga aaaccgagta gataaattat tagaaaagaa acaagattta tttgattttc
    42181 ctgaaataaa gtggcattta attggtaatt tacagcgcag aaaggtaaaa ttgattgtaa
    42241 atgaaattga ttactttcat gcgttggaca gtttaagatt agcagaagaa atccagaagc
    42301 ggttagagaa agaattatcc tgttttattg aagtaaatgt ttctggtgag gaatcaaaac
    42361 atggaatttc accagatgag cttttaccgt ttgtggaatc tttagctgcc tgcacaaaaa
    42421 tcaaggttgt tgggttgatg actatggcgc ctaaagaagc ttctgatgag caaatccata
    42481 aagtgtttgg tacgctgaaa gaattgcgtg atcttgttca agcaaaaggt tatgcccatg
    42541 caccatgtag tgaattaagt atggggatga gtcaagactt tcaaattgcg attgaagaag
    42601 gtgcaacatt tatccgtgta ggaacagcgc tattcaaaga cgaataaagg aggaacaaaa
    42661 atgccgcttt taaacagagg agcaattgct aattttttcg gattagctga tgaggaagag
    42721 tacgaatacc aagattatcc tgaaacaaca aaaaaggctg tacagccaac taaaaaaaat
    42781 acaacacaga atcaatcaac tcattctgta tctgctgcga atactcatga aaaacaaaaa
    42841 acggcgactt cacgtcctgt aacgcatgaa agtattcagc cggctgaaaa gaaaaaagta
    42901 attgaactac atcaatcacc tacaaaaact gtagcgaaag aaagaacagc ttcaagaacc
    42961 tctacacaac ctaaagcaaa tcttgctgga aaaatcactg taatggaacc aagagcttat
    43021 tctgaagcta tggcgattgc taaacgaatc attgctggag aagcaacctt agtgaatttt
    43081 catttagtag aagaaaaaca agcgagaaga atcgttgatt ttctaacagg aacagtttat
    43141 gcactagatg gagatatcaa acgtgttgga gatgagattt ttctttgtac accgactggg
    43201 atggagattg acagttcaac cgctcaatct tttgcagatg cgaatttgtt tgatctgtaa
    43261 gaggaggaac tacgatttat actattgcac tattacttca tttacttcaa cgggcagcct
    43321 atttctatag cattatcttg atggtatatg cattgctctc ttggtttcca ggcgggtatc
    43381 aatcagggct aggacgatta ttagctaaga tctgcgaacc gtatatcagt ttgttcgatc
    43441 gtctgccttt gaggattgga cccttggatt tcacaatttt aggggcaatc ctgatcttga
    43501 acttagccgt acaaggttta gcacagatcg ttatatggat aactgtccat ttagtttatt
    43561 gattgaagca acaaaatctt tattctcaga atagatagga ggggaaaatt ttggatgcaa
    43621 atgtatacca gcactttcgg gcagatgaac gtacatttat cgattcggta ggcgattgga
    43681 ttgaacaggt tcaaggacaa tatgcaccct atctgaccga atttcttgac ccaagacaat
    43741 cctatatttt agaaacactg attcgacaaa gcagtgattt gcgtttcatg ttttatggcg
    43801 gatatgaaca ggcagaaaga aaaagatgtt tgattttccc cgattattac gaacctgtag
    43861 aggaagattt tgaaacagca ttgtatgaaa tccattatcc tagcaaattt gccagcctaa
    43921 gtcatgggaa aatccttgga actttagtgg gaacggggat caaacgctct tactttggcg
    43981 atatcatctc ggacggtgat cgctggcaag tatttattgc aaatgaggtg gatcattttg
    44041 ttgctgcaca agtaacaaag attggaaagg ttgctgtacg cttggaacca atcaaataca
    44101 cagaaatgat tatgccgaaa gatagctgga cacaagaaag aggaacagtt agttccatgc
    44161 gactagacag tgtgatctct gagatttata atatttccag acagcggtcc aagcagctga
    44221 tcgaagctgg taaagtaaaa gtgaactgga cagaaaatac aagacctgat tttgtacttg
    44281 aactattaga tattatttca gtcagaggtt atggcagagt ccagatccaa gaacttgagg
    44341 ggaagacgaa aaaagaaaag tacagggtac tgttcggtgt attacgtaaa taaaataaaa
    44401 aggtggttta ctcatggctt taactccatt agatatccaa aataagacat tcccgacaaa
    44461 aatgcgggga tataatcaag acgaagtaga tgatttctta gatttagttg ttcgtgacta
    44521 tgaggaattg actcaaagaa atcgagaatt agaaaaagca gtcaaacatt ctgaagaaaa
    44581 acttgagtac ttcaatgaat taaaagatgc tttgaaccaa tcaatcatcg tagctcaaga
    44641 tacggctgat aaagtaaaaa caagtgcaag caaggaatcc gaagtgatcg ttacttctgc
    44701 acaaaacaaa gcagatgagt tagttgcgaa cgctgaaaaa cgtgcacatc aattaacaac
    44761 tgatgcagaa gaaaaagcaa gaaaaatctt gacagatgca acagaaaaag caagacagct
    44821 tgcgacagaa actgaagatc ttaagaaaaa aacacgagta ttccatcaac gtatcagctt
    44881 gatgcttgag tctcaattag aacaagtgaa aagtccggaa tgggatgaaa ttttacaacc
    44941 attctccagc tacgtgactg acagccatga agtgatcaaa gaagttttag caaaacagct
    45001 tgacaatgaa aatgatactg atgtaaactc agacacagga gtcatagaag cacctatgac
    45061 ggcatttgat acacaggcta ttgatatctc gatcgtgcct ggtgaaaatg aaaacgagta
    45121 gaacagaaaa gcactttgtt attcttaaag cgagccgatg agagtggaag ttcggtaaga
    45181 cactgcatgg tgaaagatcc tctacttgag acctgctttt ccgaaattag agtaagaagc
    45241 agcggctgac ctcgttattg gtcacagaga gtttgcacta gatagatgca actaaatttg
    45301 ggtggtacca cggcatgttc gtcccttgcg gctgaacatg cttttttgtg tccggaaaac
    45361 aaataaagga gtgtatggtt gaatgaaaat gaaagaaacc ttgcaattag gaaaaacaaa
    45421 attccctatg cgtggtaatt tgccaaaccg agaaggcgag tggcaaaaag aatgggaaga
    45481 aaatcgaatc taccaaaaaa gacaagagtt gaacgaagga aaacctagct ttgttcttca
    45541 tgatggacct ccatatgcca atggtaatat ccatttagga cattcattga ataaaatcag
    45601 taaagacatt atcattcgtt caaaatcaat gtctggctat cgtgcaccat atgttccagg
    45661 atgggataca catggattgc cgattgaaca agtattggct aataaaggcg tgaaacgtaa
    45721 agaaatgtcc atggcagaat acttggaaaa atgtcgtgaa tatgcacttt cccaagtaga
    45781 taagcaacga gcagatttca aacgcctagg agttgcagga gattgggaac atccttatgt
    45841 tacattagat ccttcttatg aagctgcaca agtacgcgta tttggtaaaa tggctgaaaa
    45901 aagctatatt tacaaaggat tgaaaccaat ttactggtct ccgtcaagtg agtcttcttt
    45961 agcggaagct gaaattgaat ataaagatgt aaaatctcca tctatttttg tagctttcaa
    46021 tgtcaaagac ggcaaagata ttcttgatga agatacagct tttgtcattt ggacgacaac
    46081 accatggaca ttgccagcaa accaaggtat tgctgtaaat ccaaaattta cgtatgtatt
    46141 ggtggaagcc gatggccgta agtttgtcgt agcgaaagat ttgctagaaa cagtccaatc
    46201 agaaatcggt tgggaaaatg taacagttct aaaagaattt gctggacaag aaatggaata
    46261 tatgactgca agtcatccat tctatgaacg tgaatcactt gtgattttag gcgatcatgt
    46321 tactttagac gctggtactg gattagtcca tactgctcca ggacatgggg aagatgacta
    46381 tatcgctggt agcaaatata aactatctgt agtgtcacct gtagactcaa aaggattgtt
    46441 tacagaagaa gcacctggat ttgaaggtat cttttatgac aaagcaaatc ctatgatcac
    46501 tgatctatta aaagaaaaag gagctttgct gaagctagat ttctttactc atagttatcc
    46561 tcacgattgg cggacgaaaa agcctgtgat ctatcgggca actccacaat ggtttgcatc
    46621 tatcgataaa ttccgtcaga atattttgga tgaagtagaa aaagtagagt gggttatccc
    46681 atggggtaaa acacgtttgt ataatatgat ccgtgaccgc ggcgattggg taatttcccg
    46741 tcagcgtgca tggggagtac cacttccgat tttctatgct gaaaatggag aagcaatcat
    46801 cacaccagaa acaatcgaac atgtggcaaa attatttgct gaacatggat caattatctg
    46861 gtttgaaaaa gaagcgaaag aacttcttcc tgaaggattt acccacccag gttctcctaa
    46921 tggcgaattt acaaaagaaa aagatatcat ggatgtctgg tttgattctg gttcttctca
    46981 tgaagctgtt ttacgtcagc gaccagaatt atcattccca gcagatatgt atttagaagg
    47041 atcagaccaa tatcgtggct ggttcaactc aagtatcacg actagtgtgg ctatcaatgg
    47101 tgtagcacca tacaaatcag ttatttctca aggatttgct ttagatggtg aaggccgtaa
    47161 gatgagtaaa tctttaggaa acatcattgc accagaaaaa gtgatcaaac aatttggtgc
    47221 agatatcctt cgcttatggg tatctagtgt agattatgaa gcagatgttc gtgtttctat
    47281 ggatatctta agtcaagtgg cagaagtata tcgtaaaatc agaaatacga tgcgtttctt
    47341 attagcgaat acagaagatt tcgatcctaa aaaagatact gtttcgtaca atgatttacg
    47401 ttctgtagac aaatatatga ccgttcgatt gaaccaagta atcaaagaaa tccgagaaga
    47461 agggtacgac aaatataact tcatgcatat ttaccgtaca gttatgaact tcttgacggt
    47521 tgatctatcg tcattctacc tggattttgc taaagatgta gtttatattg aagcagaaga
    47581 tgattatcaa cgtcgttgca tgcaaactgt tttctatcaa acagctgtag ctttgacaaa
    47641 attattgact cctatcattc cgcatacagc agaagaaatc tggtcattcc tgaaagaaga
    47701 agaggagtac gttcagttaa gcgagtttcc tggttatgaa gagtttgcaa accaagaaga
    47761 attgatggat acctggactg ctttcatgga cttccgtgat aatgtcttaa aagcattgga
    47821 agaagcaaga aacagcaaat tgattggtaa atctttggaa gcaaaactaa ctgtttatcc
    47881 aaaacaacaa gttcgtgaaa tgttaaaagc tttggatgca gatattgcac aattattgat
    47941 cgtttcacca gactactttg aagttatggc agcagatgaa caagtgccag ataacgctat
    48001 ggtatttgat gatgttgcta ttacagtgga aaaagcagac ggggaaactt gcgatcgctg
    48061 tcgccaagtt cgtaaagatg taggagtaga tgaaaaactt cctcatctat gcggtcgttg
    48121 tgcaaaaatc gttgaagact tctatcctga agctgtagct gaaggattcg aagaaaagta
    48181 aaagggaaaa gggggtgtga catgttcttt gtcacacccc cttttgttat caatcgttta
    48241 attttccacg ttcacgatac cagttatctg gttgccagat ccattcgaat ccgtcttttt
    48301 caagtagttc gaaagctgct ttaggtccca ttgatccagc tttgtagttg ggaaaatccg
    48361 gttgtgtttg gtcccaagct tttcgaatga catccacgat atgccaagat tgagcgactt
    48421 cttcccaatg agagaagttc gttccatcac cattcaacgc atctaatagt aatttttcgt
    48481 aagcttctgg gctattttca accatttctg cactatttct aaattccaat ttgattggtt
    48541 ccgtttcaaa tccttgccct acttctttcc cattcaagga taaagaaaat ccttcagttg
    48601 gctgaatata gatcgttaga acattaggtg gaagtgtcgt atcgtcacat ggttcatcga
    48661 cagatgtttt gaatacatta acaggaactt gtttgaatac gatgttgatc cgagtccctt
    48721 tttcagtcaa acgtttgcct gtacgtacat agaaaggaac acctgaccaa cggaagttat
    48781 cgatagtgaa tttgcctgcg acgaatgttt cagtctctga ttgagcatcc actttgtctt
    48841 cttcgcgata gcccttgaat tgtacaccat ccaattcacc ttgagcatat tgcccgcgaa
    48901 cgaagttttc taacacttct tcttcgttat aaatgcggat agctttcaat gctttgatct
    48961 tttctgtacg gatttctttt tcggaaaatg ctactggcgg ttccattgca agtaatgcta
    49021 caacctgtaa aatatgattt tgcaccatgt ctttaagtgc gccactatga tcgtagtagc
    49081 ccccgcgatc ttctacaccg aggttttcgc caaaagtaat ttggatattg tcgatatagc
    49141 gattgttcca ttgagcctca aaaatattat tggcaaaacg gatagctgag atgttttgga
    49201 tcatttcttt tcctaaataa tgatcaatcc tgaaaatatc ttgttctggg aatacttctc
    49261 tgatttgctg gttcaattca aaagcagact ggtaatcaga accgaacggt ttttctatga
    49321 tcaaacgatt gaagccattt tttgtcataa tattttgtga tttcaaatga gaaacgatcg
    49381 ttccaaaaaa ttgaggtgcc atagccaaat aatagacacg gttaccgttt aattgatagt
    49441 gtccatctaa tcggtcagac aattctttta atgtctgata atgcgttgaa tcatttacat
    49501 catgggattg atagtaaaaa tggcttgcaa aggtagttgc ttcttcatct gaaggattca
    49561 atgcacggat tgtttctttg acaacttcac ggtagtgctc atcgctccag ggacggcgag
    49621 cggttccgat tacagcaaaa tgctcgccta aatttccttt tctgtaaagg cggaacaaag
    49681 acgggtacaa tttacgctgg gctaggtcac ctgtaccgcc gaatatcgta aacaatacat
    49741 ttttttcatt agacatgtgg atacactcct ttatatttta tcgacaaaaa tcgtgctggc
    49801 cgccttaaag ctgacgggga ttttctttct atcattttgg atagtgatcg gtccttcata
    49861 agctgcgatg tcaacgatct catattcttc gttgatattc aggtcaatag tgactaagta
    49921 atccaacaat tctttttcat caagcactcg tgcgatacgc acttttgtac cgacaggata
    49981 ggactccaat gtttgtcggc gttcttcatg gacaacttcc tcttttttag gaatcgatcc
    50041 tccatgagga caatgttcag gatgattcaa atagttttct aaatgatctg caagatgttc
    50101 cgaagtcaca tgttccaata cttcagcatc atcatggact tcattccagc tatagtctaa
    50161 atgttcaact aaaaaaactt cccataagcg atgtttgcga atcagactgc tagctttttg
    50221 cagtccgctt gcagtcaatt gtacaccttg ataaggggaa tgttctacaa gctcttcttt
    50281 cacaagttta gaaatcattt cactgacaga tgcagctgag acatctagac tagatacgat
    50341 ttgtttattg ttgactttcg ttgtatctcc acctagttct aaaatgattt tcaaataatc
    50401 ttctcgattt ggtgtcatat tcgatcatcc tttcatggta aaaaaccatc attagtaagt
    50461 gtaacaaaaa aaacgttaat tgactatttt tctaaataaa aaacaaagga tttttagacg
    50521 tttttacaac aaaaaacgaa agatgataga gaagactcat tctttcgttt ataaaggata
    50581 tatatataca ttgggaggga tctatttttc agataacaac ttatatgaag tgtctgcaga
    50641 ttcgtcataa agagtttgtt cgggataaga aaagataaga taaaaaccat actgatcacg
    50701 tacgactaaa cgatattcat ctatttctac cacagatgtg ccaagagact gaccgttgat
    50761 caaaaacgtg taatcagatg taatctgtaa ttgttgtttt tgctggctat cactaaagaa
    50821 ccatgtacct tgatactttg agagtccttc tgacttttcc gcttgttttt tctttttcaa
    50881 gtaaataaaa cttgagagaa cggtaatgag tgaaccaaga aaatagatca ttttttttat
    50941 caccaaatca ctcctttatg agaaaataat cgcatagaca tattctttct tctttatttc
    51001 attgtaccga ataaaagcta aaatttctct aagactttcg actggttttt ttactgtttt
    51061 tctacaaatt atgataggat acatagtagg aagaaaggtg acataatttg aagtatcagc
    51121 aagtggaaat agcttttttt attgaccgcc cgaatcggtt tatagcacat tgtatgaaaa
    51181 aagacggaga aataattatt gcgcatgtca aaaatacagg tagaggaaaa gaggtcctga
    51241 taccaggcgc tgaagtggct attgtatttg ctcctggacc taaacggaaa accgcctatg
    51301 atctgattgc tgtaaaaaag aagggtgact ggtttaatat cgatagtcaa ttgcctaatc
    51361 ggctggcaat tgatggtatt ttagacggca cgatcctttt accaggatta actggtccat
    51421 ttacggtata caagcgtgaa gtaacttatc ttcactccaa atttgatatt tatttagaga
    51481 cggtggtcgg acaaaaagca tttgttgaag tcaaaggtat gacacttgaa aataaagcga
    51541 ttggtgcttt tcctgatgcg cccacactca gaggattgaa acatatagga gaattaacgt
    51601 atgccgcaca agatggttat gccgcttatg tcttgtttat tgctcagttc gaacatcttc
    51661 atctggcaac gatccatgaa gaaatgcaac cagcactagc tgatatggtt cgacatgcgc
    51721 agcaaagcgg agttcaaatc ctcgcctata attgtcaagt atcggcagat gaggtaactg
    51781 tcaagcaggc cattccattt gatgtagata tcccttttga agatcctata caatagaaag
    51841 aaggtaaaat aatgatcaac ttaggtatta tcggaacaaa ttggattact catcaatttg
    51901 tccaagcagc tttaagcaca aaaaaatatg aattgacagc ggtatattct agacatttgg
    51961 aaaaagcaca ggaatttggt tctcattatg aaggggatat cgaattcgca acagatttga
    52021 agactttttt caatattact catatggaca ccatttatat tgcttctccg aatagtcttc
    52081 actttagtca agctaagcaa gcaattttgg caggaaaaaa tgtgatagtt gaaaagccag
    52141 ctttttcgac gccaaaagaa atggatgaaa tcatccattt ggcaaatgaa cacaatgtgt
    52201 atttttttga agcagcaaga aatatccatg aatcaggttt taaaaagatt gccgattact
    52261 taccattaaa agacgagata atcggtgcaa atttcagcta tatgaaatat tcttcgcgct
    52321 atgaccaagt attagatgga gaagaaccga atatcttctc gactcatttc tcaggtggtg
    52381 cattgatgga tttaggtgtt taccttgtat atgcagctgt tgcttggttt ggaatgccaa
    52441 aagaagtcca ttatttccca agaaagattt ctactggtgt agatggtctt ggatggggga
    52501 tcttacgtta tgagaatttt gatgtatcga tccagccagg taaaatcggt gattcttatt
    52561 taccgtcaga gatttatttt gactcaggaa cgctagtgat gaatggagtc aatgcaattg
    52621 aacaagcaca atttcatgat cgcgcgcata aagaaatcat tgatttggcg gtaacagctg
    52681 cagaaaatcc aatggcagat gaagcagagg attttgccaa tgtcatggct catccgaaag
    52741 atccagcttg gggattgttg tatgaagagt ggatcgagct agctagaaat gtgaaccaag
    52801 tgatttatga tttaagaaaa aatggcggca tcaaattcga tgcagataat gaggaagatt
    52861 tttaatgaac gaatttgtac aagtatttcc cgaaaattgg cagaaaaaat atgaggataa
    52921 agggtttatc tcaccttctc ttattcaacg agcagttttt cagccactaa gtaataaaca
    52981 aagtatagta gcggtttctc ctacaggttc aggtaaaaca ctggcttatc tttggcctct
    53041 actactaaac gtggaaccag gtgaagcaag tgcgttggtc atttttgctt cttctcaaga
    53101 actagctatt caagtagcag atgttgcacg tgaatgggga aaagacaaag agttgaaagt
    53161 ccaatctttg gtgggcggag ccaacgtcaa acgtcagatt gagggtttga aggaaaaacc
    53221 agaaatcttg attggtacac caggaagaat attggaattg atgaaagcaa aaaaaataaa
    53281 agcacatcaa gtgaaaacaa tggtatttga tgaagcagac cagctatttg acgctgggaa
    53341 cagccaaatc attgatcaaa ttcttcatca agctccaaca gagtatcagc tagctttttt
    53401 ttcagcgaca gcagatcgtt cccttgaaag cattgaaaaa atcactggga aaacgtttga
    53461 gacaattgac gtgacagcag aggacgattc tcgaaaagga ctgagacatt actttttacg
    53521 tgtaccgata cgaaagaaag atgactactt gagacgtctc acgcatatag aagattttca
    53581 tggtctgatt ttctttaatc aactgactga attaggcgta atggaagaaa aattgctgta
    53641 tagaggtgta ccggtaggaa gtctggcctc tgatcagaac aagctactcc gaaaagcagc
    53701 gctagaacaa ttcaaaaaga aaatggtcag agcacttttg accacagatg tggcggctag
    53761 aggtttagat attacaggtc ttccttatgt agtgaatgca gaagttccgt taagtgaaga
    53821 agcttatttg catcgttcgg gaagaactgg tcgaatgggt aatgaaggaa tagtaattac
    53881 actcgtacaa gataataatc taagagattt aaaacggctt ttacgtccat caaatataac
    53941 acttgaagag atttttttat acggaggtaa gctgcaaact gagcagccaa ccaaagaaga
    54001 tgcagctcct gcagtaaata aaaagtatgc gaaaactttt tctggaaagg agcagaaccc
    54061 agaatataac aaagaaaaaa taagaaaaaa agttaaaaat aaatcaaaaa aagagagaaa
    54121 taaaggagca aggcgcaaat aagcgccttt ttatgctatg attatgaaaa cgggaacaaa
    54181 attggttgcc agaggagggg ttatgtgaaa gaacgtcaag aagagatctt gcagctattg
    54241 acagaaaacc aacaaggatt gacagcaagt gatgtagctg agcgcttaac tatcgatcga
    54301 agcaatgcaa gccgatattt aagtgaacta tacaaggccc atcatattgt aaaaaccgct
    54361 ggccgaccag tcgtttactc tttgccaacg gaaaagtcaa aatctgatga agtgcatgtc
    54421 gactcttcta cgcaagtgac ttttgaaaca ctcgttggag aaaatgattc tttaaaagtc
    54481 agcatacagc aggcaaaagc tgcgattctt tatccaccgc gggggttgca tacgattatt
    54541 tttggagaaa ctggtacagg gaagtcgatg tttgctgaat gtatgtatca ttttgcaatc
    54601 gattcagaga tgctttctgc agatgcgcca tttgtttctt tcaattgtgc ggattatgct
    54661 caaaatccgc aactgctctt tggacacatc tttgggatca aaaaaggggc ttacacagga
    54721 gcagctcaag atagtcccgg tctaatagcc aaagcagacg gcggtatatt gtttttagat
    54781 gagatccacc gtttaccgcc ggaagggcag gaaatgctgt ttactttcat tgataaaggt
    54841 atttaccgtc cgcttggaga aagtgcacaa gtacatgaag catctgttca gatcattggg
    54901 gcgacgactg aatcttcaga aagtttctta tctacgttca atcgacggat tcctatggca
    54961 atcaccttgc cgaatcttgc ttcacgttca ctggatgagc gttatgaaat catctctctt
    55021 tttatcaaac aagaagcgaa tcgtttgaat caacgtatcg atatcgaaaa agaagcgatt
    55081 cttgccttca tgctttatga tgcggaagga aatatcggtc aagtgaaacg agacttgaaa
    55141 ctagtctgtg caaaatcttt tttacattac cgtactcatc atgagaaaaa gctaatcatt
    55201 cgaaaagaag aactttccct tcaagtacaa aaaggattac tgaaaatcaa ggaaataccg
    55261 gaacgtctcg accgattcat ggacagcaaa agtcagtatt tgacatttga accaggattt
    55321 gctgatgttg tctggtcaca agatccagaa cgaaacatgc aggtctataa cgatattgag
    55381 gaaaaagtca cttctctttc cgaaaccggt gtggagaaca ttgatctaga gaccctcatt
    55441 tcaaaagatg tggatgctta tttccagaca tatgtcaaag aattgactaa aagttcgatt
    55501 cccaaagatc ttttgccaga tgaaatctgg cggttaacca atcaattgta tgatgtggct
    55561 gaaaaacggt tagaccgtat ttataatgaa aaagctcgtt ttgcttttgc tttgcatctg
    55621 caaagtacat tggatcgtgt gagagaaggt catatgatcg tccacccaga tttgaataac
    55681 gtacggaaaa attttaagag ggaattccag gtagcaatcg atctttcaac gatcattgaa
    55741 gaagaagaac aagttgagat tccttttgat gaaatcggct ttattagtat gtttctttct
    55801 atcaatctag gagaaagtga gcctgttaaa gaaaacaaag tagatgttct catattgatg
    55861 cacggcagac agactgcttc aagcatgttg gagatggcac aagaactatt aggaacaaaa
    55921 gtagggaaag cttttaatat gccactggtt atggaagttc aaacaatgta tgaagaagtg
    55981 ttacaatatg tcaaaaacta tcaagagcat ttgacgaatg gcctactctt gttaacagat
    56041 atgggatcat taaacacctt cagtgatttg atttatgaag agactggcat tcggacgaaa
    56101 gctatcacga tgacaagtac catgattgtg atagaagcga tacggatggc aagtgttgga
    56161 agaagcttag aagatattta tcaaaatatc cagctttcct ttgaatcgat cgtacgtgat
    56221 cagttcaaag cggaaaagtc tgaaacttta cagcgtaaaa aagccgtaat cgtgacatgc
    56281 tttactggtg aaggagtagc tgccaaatta tatcagcgta ttatgccagt gattaatcaa
    56341 aataaggtag aactgatcca gatgcagttt ttagaacgtg aatcattcaa aaaacacatc
    56401 gatgccttat tggaagaata tgagatcaaa gcaattgcgg gaacagttga tgttgattat
    56461 caaaatatcc catttttttc agcctatgac gtttttgatg atgaaaaact aaatatttta
    56521 aagcgtatag ctgatgatga gatcccgaca gctaaaattg tcgattcact tgctggatca
    56581 ttaagcgatg tcttttctat tgatgaactg atccgttcat tgcaaaagac agttcagcag
    56641 atccagaccg atttgtcgat tatcgtggaa cctggagtag atactggaat cgttatccat
    56701 ttagccttca tgttagacgg attgatcaaa caagaaagcc atcggcaatt tgagaaactg
    56761 tctattttct caaaaattta tcgtttggaa atggatatcg ttcgggcaaa cttgatggca
    56821 ctagaacgaa aatatcaggt aaaaattcca gaagatgata tcgcatttct tacacaaatg
    56881 tttcttgaaa ataaaataaa aaaatgaaca cactaatcat actgtttaca cactaaacat
    56941 ctgtgtgcgc actataaaaa agagctaaaa agcatgaaaa cgctatttca gctctttttt
    57001 ttattggcat gcttcttgcg ttatataaaa gtgtaaaaaa ataaaaggag tgttaaaaat
    57061 ggcaaaaaaa acaatcatgt tagtttgttc agcagggatg agtacgagtt tattggtcac
    57121 aaaaatgcaa aaagcagcag aagaaaaagg gatggaagcc gatatttttg cggtctctgc
    57181 ctcagatgca gataataatt tagaatctaa gaatgtcgat gtattattat taggtcctca
    57241 agtacgtttc atgaaagctc aatttgaaca aaaacttgcg cctaaaggta ttccattaga
    57301 tgtcatcaat atgcaagact atggcatgat gaacggagaa aaagttcttg cacaagcaga
    57361 aaatctaatg aaataaatca agtatgcagt tgggggtata acaacgtgga agatcaacaa
    57421 aatctagaag ccatcatggg gttaatcatg tttggcggta atgcaaagag cgatgcaatg
    57481 gaagcgattg cggctgctaa aatcggtgat tttgagttag cggataggaa aatcgctgat
    57541 gcagaagaat cacttataca agcgcatcat gcacaaacag acatgttgac acaagaagca
    57601 caaggaaatc atatgcaagt gacattattg actgttcaca gtcaagatca cttaatgaca
    57661 tctattgcgt ttactgattt agcaaaagaa atcatcgaac tttatcgtcg cgtatacagc
    57721 gaataaatac aacttctttt ttgaacggaa gaagaaaaat ttttgaacgg attttttatt
    57781 ttgttataag gagaactctc tttatataaa aaacttatag gaggaagtaa aaaatgaacg
    57841 gactatcaag ttggatggag aaatacatcc tgccggttgc cgcaaaaatc ggagcgcaaa
    57901 aacatttagt tgctttgcgt gatgctttta tcggcacact gcccgcaaca atggcgggat
    57961 cagtcgctgt aatgatcaat gcaattattc gtgatttacc gcaacagttt aacagtacct
    58021 atgctgacag tttagcagca gacaaaggaa tctttgccgt attgaatgtc atcatcggaa
    58081 tcaatggttt tgtctggaac ggtacactag caatcgccgg attgatcttt gccttttctt
    58141 gggggtataa tcttgcccga gcttatggag taaatgatct agctgggggg atcgttggtt
    58201 tagctacctt gattcaaggt atttcctttg gtccagcatt atcagcagca ttagatgtga
    58261 atgttccttc aaatgtcgca gatgcaatca atagttctga gattggtgca acgattgcag
    58321 atggcacact gtcagttgga ttatggggtt ggctaaaact agatcatttg aatggaaacg
    58381 cttattttac agttatgatc atgggagcta tttcagtcat tattttttgt aaattgatgc
    58441 ttgccaacat tacgatcaaa atgcctgatt cagtaccacc agcagtatct aaagcatttg
    58501 cagccatttt accagcgacg atcgctttat acgtcattgc tattatcaat tatatcgtag
    58561 gtcgtgtagc tgatgggcaa ctgatcattg atttggtgca aaaatatatt gcagaaccat
    58621 tcctaggatt atctcaaggg ttgggagcag tattgatcgt cacagtattt gttcaagtat
    58681 tctggttctt tggtattcat ggaccaaatg tattggcacc agtgctagag ggaatctggg
    58741 gacaagcaca actaatcaat attgatattt tccaaaaagg ttataacgga aaaactggta
    58801 caccagccgt gttagatgca atcgaagatg gaaaagctta tatgtgggtc cgcggatcgt
    58861 tcgatgcttt tgcatggttt ggcggatcag gcggaacgat cgtcttactg atagctatct
    58921 tgattttctc taaacgtgca gattatctaa cagtagctaa attatccatt gggcctggta
    58981 tcttcaatat caacgaacca gtgatgtttg gtctgccaat cgtattgaat gcaatcatgt
    59041 ttatcccatt cttgattgct ccagtagtag caacagcaat tggatggaca gctacttatc
    59101 ttggcctagt cgctccggta tcgcagcaag tcacatgggt agtaccgccg ttcttattgt
    59161 cattcttagc aacaggagcg gattggcggg caccgatcgt aacattagta tgtatggtcg
    59221 tgactttctt gatttgggca ccatttgtca tctctgcaaa caaaatggac cctgccgcag
    59281 gcgaacaaca ataagatgaa cgtgaggagc gggacgcttt aagtttcgtt cctcataatg
    59341 aattttcgag gaggaactga aatgattaca attgccagtg caatgcaaca agatgaaatc
    59401 aaagaattgt tagaaggata caatgaagaa ggaatgactt ttacatttaa agaaaaacaa
    59461 ggaatcaagc tgttctttga aacaagtgca gaagatccgg aagttgcggc taaaaaagca
    59521 aaggaactaa tcaaagcaca gccttgggga acagtccttt attttcaatc aacagctgtt
    59581 tagaaaatgg tttaaaaaga taggatggtt gagatgataa aaaacggttt atttttatgt
    59641 agtattggtt ctttgatcgt gttgtatagt ttgaatccac attcgcaaag gtatgatttg
    59701 tatagtatgg tcacaggtct ttttctaaca ggattaggaa tctatttctt catgaagggg
    59761 aaaaaaagag aagaagcaaa ggagaaaaat aactaatgga aatcacgaaa aaattaaata
    59821 ttccagcatc gtttttttac gataaagtta tggactctgt ccttttcgat gtacgtaaag
    59881 cgaccggcaa atcagtaact agaaaacagc tgaaaaattt ggagtacgtc aaacaattct
    59941 cgcaaaataa ccgggctcgc ataaaaatcg aagaagtcat tgaaaaccag tcttataagt
    60001 ttcgtacatc gacaacgaag aatgaattcg tcgttcatta tcaaatcaaa ccattggacg
    60061 attcgacctg tgaggttcat tacacagaaa aaatggagtc ttatggcatg cttcaaaagt
    60121 tgaatgatat gcttttagga acaatcttga tgtattttaa acggagacgt ttcaaaaaaa
    60181 tgctagatat gatcgaagca acgtattaaa tcaatgaaac atggtactat ttagttattg
    60241 aaaaatgaag taagctagaa ggaatttttc ataggaagat agatctttcc tgcgaatcct
    60301 cactagcttt tttgtaggaa ggggcagcaa aatgacaaac aaagtaagag agctaggtgt
    60361 atcttcgatt gaggaaatgt ttgatttagc cacttatgca tttaatgggg cagatacacc
    60421 tgaaaggcga gaacgttttc gtacacttgc ggagaattct tggaattacg gattttttga
    60481 tgaggaggat cggctaacaa gtcaagtcat ggcgacccct tttccagtga atttttacgg
    60541 acaagagtac ctaatggccg gcattggcta tgttgcttct tatccagaag caaggggaca
    60601 aggcgggatc aatcagatca tggaaaagat ccttgaagat tgccgtgatc gtaaagtaag
    60661 cttatcttat ctggcgccat tctcctatcc cttctatcgg cgctatggct atgaacaatc
    60721 atttgataaa atcagttatc aactaaacag tcgtgatgtg ccgtttatca aaaaaaaatc
    60781 tggaaaaata aagcgtatgg attttgaaga tgcaaaagct gcaatcagaa gaatttatga
    60841 acagatgccg gaaaaccaac gagctggtct gaagcgcgaa gactggtggt atacgtataa
    60901 attcaagcaa aaaggcaatt accagtttgc tttgcatttt gatgataatg aggaagcaac
    60961 gggatatatc gtttatcagc ttgcaacgtc ttcctttatt attgcggagt ggggattttt
    61021 ggatcatgat gctttttgca gccttgttcg cttcgtaagt tctcacaatg gtgcttttga
    61081 aacatttgaa tattcatgcg gctacggcgg taatgatcaa aattatctat tagaaaatcc
    61141 atttgcttct gtaatgatca caccttatat ga
//
LOCUS       DACDIH010000011        51619 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDIH010000011 DACDIH010000000
VERSION     DACDIH010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.011"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(61..2424)
                     /locus_tag="IV281_000681"
     CDS             complement(61..2424)
                     /locus_tag="IV281_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAP8779717.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2872..3093
                     /locus_tag="IV281_000682"
     CDS             2872..3093
                     /locus_tag="IV281_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8779718.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="IV281_000683"
     CDS             complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="IV281_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP8779719.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(3575..5881)
                     /gene="infB"
                     /locus_tag="IV281_000684"
     CDS             complement(3575..5881)
                     /gene="infB"
                     /locus_tag="IV281_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP8779720.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(5894..6205)
                     /locus_tag="IV281_000685"
     CDS             complement(5894..6205)
                     /locus_tag="IV281_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP8779721.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(6202..6495)
                     /locus_tag="IV281_000686"
     CDS             complement(6202..6495)
                     /locus_tag="IV281_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP8779722.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="IV281_000687"
     CDS             complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="IV281_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP8779723.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="IV281_000688"
     CDS             complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="IV281_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP8779724.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(8333..12685)
                     /locus_tag="IV281_000689"
     CDS             complement(8333..12685)
                     /locus_tag="IV281_000689"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP8779725.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(12897..14606)
                     /locus_tag="IV281_000690"
     CDS             complement(12897..14606)
                     /locus_tag="IV281_000690"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP8779726.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="IV281_000691"
     CDS             complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="IV281_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP8779727.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(16103..16903)
                     /locus_tag="IV281_000692"
     CDS             complement(16103..16903)
                     /locus_tag="IV281_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP8779728.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(16900..17712)
                     /locus_tag="IV281_000693"
     CDS             complement(16900..17712)
                     /locus_tag="IV281_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP8779729.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(17907..18464)
                     /gene="frr"
                     /locus_tag="IV281_000694"
     CDS             complement(17907..18464)
                     /gene="frr"
                     /locus_tag="IV281_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP8779730.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(18467..19189)
                     /locus_tag="IV281_000695"
     CDS             complement(18467..19189)
                     /locus_tag="IV281_000695"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP8779731.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(19325..20206)
                     /locus_tag="IV281_000696"
     CDS             complement(19325..20206)
                     /locus_tag="IV281_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP8779732.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="IV281_000697"
     CDS             complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="IV281_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP8779733.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            21446..21925
                     /locus_tag="IV281_000698"
     CDS             21446..21925
                     /locus_tag="IV281_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP8779734.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            22142..23233
                     /gene="phnW"
                     /locus_tag="IV281_000699"
     CDS             22142..23233
                     /gene="phnW"
                     /locus_tag="IV281_000699"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP8779735.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            23226..24011
                     /locus_tag="IV281_000700"
     CDS             23226..24011
                     /locus_tag="IV281_000700"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP8779736.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(24357..26048)
                     /locus_tag="IV281_000701"
     CDS             complement(24357..26048)
                     /locus_tag="IV281_000701"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP8779737.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="IV281_000702"
     CDS             complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="IV281_000702"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP8779738.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(27532..28551)
                     /gene="argF"
                     /locus_tag="IV281_000703"
     CDS             complement(27532..28551)
                     /gene="argF"
                     /locus_tag="IV281_000703"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP8779739.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="IV281_000704"
     CDS             complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="IV281_000704"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP8779740.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            30332..31033
                     /locus_tag="IV281_000705"
     CDS             30332..31033
                     /locus_tag="IV281_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8779741.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(31605..32621)
                     /gene="tsaD"
                     /locus_tag="IV281_000706"
     CDS             complement(31605..32621)
                     /gene="tsaD"
                     /locus_tag="IV281_000706"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP8779742.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(32618..33082)
                     /gene="rimI"
                     /locus_tag="IV281_000707"
     CDS             complement(32618..33082)
                     /gene="rimI"
                     /locus_tag="IV281_000707"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8779743.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(33089..33631)
                     /gene="rimI"
                     /locus_tag="IV281_000708"
     CDS             complement(33089..33631)
                     /gene="rimI"
                     /locus_tag="IV281_000708"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8779744.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(33615..34439)
                     /gene="tsaB"
                     /locus_tag="IV281_000709"
     CDS             complement(33615..34439)
                     /gene="tsaB"
                     /locus_tag="IV281_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP8779745.1"
                     /translation="MYHCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDKIIVPMFDARRKNVYAGAY
                     RWKENRLEIVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFYEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(34528..35508)
                     /locus_tag="IV281_000710"
     CDS             complement(34528..35508)
                     /locus_tag="IV281_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8779746.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(35532..37016)
                     /gene="galT"
                     /locus_tag="IV281_000711"
     CDS             complement(35532..37016)
                     /gene="galT"
                     /locus_tag="IV281_000711"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP8779747.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(37028..38017)
                     /gene="galE"
                     /locus_tag="IV281_000712"
     CDS             complement(37028..38017)
                     /gene="galE"
                     /locus_tag="IV281_000712"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP8779748.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            38266..38433
                     /locus_tag="IV281_000713"
     CDS             38266..38433
                     /locus_tag="IV281_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP8779749.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(38495..40306)
                     /locus_tag="IV281_000714"
     CDS             complement(38495..40306)
                     /locus_tag="IV281_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP8779750.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(40303..40668)
                     /locus_tag="IV281_000715"
     CDS             complement(40303..40668)
                     /locus_tag="IV281_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP8779751.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(40831..41226)
                     /locus_tag="IV281_000716"
     CDS             complement(40831..41226)
                     /locus_tag="IV281_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8779752.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(41244..42206)
                     /locus_tag="IV281_000717"
     CDS             complement(41244..42206)
                     /locus_tag="IV281_000717"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP8779753.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNIYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(42206..42418)
                     /locus_tag="IV281_000718"
     CDS             complement(42206..42418)
                     /locus_tag="IV281_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP8779754.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(42439..43137)
                     /locus_tag="IV281_000719"
     CDS             complement(42439..43137)
                     /locus_tag="IV281_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP8779755.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(43157..43699)
                     /locus_tag="IV281_000720"
     CDS             complement(43157..43699)
                     /locus_tag="IV281_000720"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP8779756.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(43831..44835)
                     /locus_tag="IV281_000721"
     CDS             complement(43831..44835)
                     /locus_tag="IV281_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8779757.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(44832..45821)
                     /locus_tag="IV281_000722"
     CDS             complement(44832..45821)
                     /locus_tag="IV281_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8779758.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(45818..46624)
                     /locus_tag="IV281_000723"
     CDS             complement(45818..46624)
                     /locus_tag="IV281_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8779759.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            46790..47746
                     /locus_tag="IV281_000724"
     CDS             46790..47746
                     /locus_tag="IV281_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8779760.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(47823..48341)
                     /locus_tag="IV281_000725"
     CDS             complement(47823..48341)
                     /locus_tag="IV281_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8779761.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(48429..48578)
                     /gene="rpmG"
                     /locus_tag="IV281_000726"
     CDS             complement(48429..48578)
                     /gene="rpmG"
                     /locus_tag="IV281_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8779762.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            48806..49252
                     /locus_tag="IV281_000727"
     CDS             48806..49252
                     /locus_tag="IV281_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP8779763.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(49447..51342)
                     /gene="asnB"
                     /locus_tag="IV281_000728"
     CDS             complement(49447..51342)
                     /gene="asnB"
                     /locus_tag="IV281_000728"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP8779764.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT        14524 a        11022 c         9054 g        17019 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt tcgctcctca
     2641 tcatgcataa tttgctgaat gacgctaggc cagttaacac attgttggaa aactggggct
     2701 ggatcgagca attcctttcc tgattgatcc gttttatgta aaagtctctg ttatctttgt
     2761 caatatgtga attggtatcc cagctactat gctaatttct acagggatta tccaaaattt
     2821 ggatcgtgac caaatcgaag cggcgtaatc ggaaatatca ataacttcaa cgtgattttt
     2881 cttttaactg tcaacggacc gacaaactca aacttttatg gtgcaggttc cactgatctt
     2941 ctagttactt ggctatacct gactgtcact aaaatagatt acaatctagc ttccgttatc
     3001 ggtatcttga tctttatcta tatgcaaaaa tttatatact atagaaaaaa gaaagtcgaa
     3061 ttttttactt ttctttttct cttttttggt tagctgacac gctaccagac taaagtcatg
     3121 gaactcttag gtaatacgtc ttaatgaaaa caaaaaaatc ccgcaacccc tgatttcaca
     3181 ggatttgcgg gattttttga ttagtctttg ttcaagtcgc gaagcaattc atcgatatgg
     3241 ttcccataga caactgattc atcaagttca aagaacaatt caggtgtctt ataaatactt
     3301 agattgtgcc ctaactctcg acgaatcaat cctgatgctt tattcaatcc atcttgtgct
     3361 tttttcttat ctgatgctaa gtcagataaa atgctgtaat agatcgttgc ttgctgtaag
     3421 tcccccgtaa ctttcacatc tgtgatcgtt acgttctcca cacgagggtc acggaccttt
     3481 ttgcgcaaaa tatcattgac ttcttttaag atttcttgac cgactcggcg atcacggtag
     3541 ttagccataa tgattggcct ccttttatca gtttttagtc atttttgact tcttccatga
     3601 taaatccttc aattacgtca tctactttga tgtcattgaa tttttcaatc atcgcaccac
     3661 attcataacc cattttgact tctttcacgt catctttgaa tcgtttcaga cttgcaagtt
     3721 ggccttcata gattacgatt ccgtcacgga ttacacggac accgctatct ctacgaatat
     3781 atccttctgt tacataggca cctgcaatcg taccaacttt agaaactttg aaggtttcac
     3841 gaacgatcat ttgaccggtg attttttctt caaattcagg gtctagcatc cctttcatcg
     3901 cagtttcgat ttcttcgatg gctttataga tgatacggtg aagacgaata tcgacttctt
     3961 cagtatcagc ttgcactttt gcctgcggtg taggacgaac gttgaaaccg atgataatag
     4021 cgttgctggc tgctgctaag gttacatcac tttcattgat tgctccgaca gctgaatgga
     4081 cgatttttac acggacacct tccacatcaa tcttcttcaa tgatgcagcc agtgcttctg
     4141 ctgaaccttg tacatctgct ttgatgatta cgttaacttc tttcaattcg ccttctttca
     4201 ggctttcaaa taagttgtcc aaagtcacac ggcttgtggc agcacggttt tccaataatg
     4261 cacgttttgc gcgttcttca cccgctgcac gtgctgtctt ttcgtcttcg aagactacga
     4321 aacggtcacc tgcttgaggt acatcattca agccagtgat ttctactggc gttgcaggtc
     4381 ctgcttcttt atcgcgacgg ccaagatcgt ttgtcattac acggacacgt ccgtaagtat
     4441 taccgacaac gattgggtca ccaacgtgaa gtgttccttc ttggacaagt aaggttgaca
     4501 caggaccttt acctttatca agacgtgctt cgatcactgt tccgattgcg cgttgtgttg
     4561 gatctgcttt taaatcttct acttctgcga ctaacaggat catttccagc aattcttcga
     4621 tgttttggcc gaatttcgct gagatctcaa caaaaatcgt ttctccaccc catgcttcag
     4681 gaattaattc atactcactt aattcttgca taacatgctg aggatttgca cctggtttat
     4741 caattttatt tacagcgacg atgattggaa cgcctgcagc tttggcatgg ttgattgctt
     4801 caactgtttg tggcatgacg ccatcatctg cagcaacaac taagatcgtg atatctgtga
     4861 tacttgctcc acgtgcacgc atacttgtaa aggccgcatg tcctggtgta tccaagaaag
     4921 tgattggttt accatcaata tcgatctggt aggcaccgat atgctgtgtg attccgccgg
     4981 cttcgccgct tgttacacgt gaatggcgca atgtatccaa taaggttgtt ttcccgtggt
     5041 ctacgtgtcc cataatcgtt actactggcg gacgtgagac taatttatct ggatcgactt
     5101 cttctgcctc gaagaattta tcgatatcag caatgtcaac ttggactttt tcttgtggtt
     5161 ccatgccata gtccgtcgct aacagttcaa tcgtatcttt atccagtgct tggttttgat
     5221 tgaccatgac accaagcata aataattttt taatgatttc tgctggctca cgatgaattt
     5281 ttttcgcaat atctgctacg ttcatacctt cagtatactc taatacatct ggcaattcac
     5341 ggaatttgcg tggtggtaca gctggtttag ttgattgttg ctgtttgcct tttttacctt
     5401 ttttattata acgattacgg ttattattat taaatttgcc gcgattgtta ttgtttggtt
     5461 tgttgttatt gttagtacgg ttttgattgc cgccttggct cgttccttga cgattttggc
     5521 tgctggtatt tcccgtatta ttttggtttg ttgttggttt ttttccttga ttcacttgac
     5581 cgctcccttg accgcgatct tgataactgc ggttattttt atttttttgt tgattgttcg
     5641 ttgtttcttt tttacgttga tcgttcggct gttgattggc tggttttctg cttgcttgtt
     5701 gtgcagcttg ttgtttcgtg cttgggtttt tgaacgcttg ttgcaatttc ttctcatcac
     5761 tgctagtaat cgcacccata tggtttttca catcgatgcc aagttgttgt gctttttcca
     5821 cgacgtcttt actagatttt ttgagttctt ttgctaattc atagattctt ttgttgccca
     5881 tgcaatcacc ttcctaacct tcaattagct ccttggtctt cttcgcaaat ccggcatcca
     5941 ttattccgaa taccttgcga ggttttccga tcatctgcgt gatttctgct tcagaaaata
     6001 gctcaaagca aggaacttca taatatgaac tcttatcttt tatctttttt cttgtattct
     6061 cacttgcatc ggttgccaca aacaccaatt ttgctttttg tttgcggatt tcagcaatgg
     6121 tcagttcttc acccgtgatt agttttcccg cgcgcatcgc tagaccgatg agattcatgg
     6181 ctttttgccg attaactgta ttcattttcc aaataactct ttacgtgctt tttggtgagt
     6241 gacatagtca agaagttctt gataaaactc atctgttaat gttgtttcga gtactcgatc
     6301 gagaattttt ttatcccaag cttgttgtac ttcttccggc tcaagtgaga catacgctcc
     6361 tcgtccaggc atttttccag ttggatcgat cgatacttcc ccttcttttg aacgggtgat
     6421 tcggaccatc tcttttttag gtttcatttc acctgaaacc acggatttgc gtaaaggaat
     6481 tttcctttgt ttcatctctt tgcctcctta ctaaagttaa gcttctggtt tttcttcgac
     6541 cgtttcattg ttgaatgcaa ttgtttcata ttcatcgtct gttaaatctg actgaacgat
     6601 tgcttcatca tgcagttctt ctgtttcttt ttgttgttct tgattttcat aaaattccgt
     6661 catgtcagat tctgatttga tatcgatttt atggttagtc aatttagcag ctaaacgtgc
     6721 attttgtccg cgtttaccga ttgctagcga taattggtag tctggaacga caactgtgca
     6781 ggcctttgga tttgtctcat cgaagatcac atccatgact tgtgcaggat tcaatgcatt
     6841 tgcgatgaat acagctggat cttcattcca ttcgacgata tccatgtttt cgccttttaa
     6901 ttcattgacg attgcttgaa ctcgttgtcc ctttggcccg acacatgtac cgactgcatc
     6961 gatatttgga tcatttgagc gaactgccac ttttgaacga tcgccagctt cacgggcgat
     7021 gctgacaatt tcaacgattc catcatagac ttctggtact tcttgttcaa ataagcgacg
     7081 caataggtct ggatggctgc ggctaacaaa tacttgcgga cctttagacg tattttctac
     7141 tcgggaaaca taaactttga ttcgatcatg tggttgataa aattcatttg gcatttgatc
     7201 ttgtttagaa agaactgcct caattttacc aagattgaca taaatataac gtttatcctg
     7261 gcgttcgacg attccttgca tgatgtcttt ttcataagca ctgaattcgt tataaataat
     7321 cgttctttct gcttcacgta cacgctgtaa aataacttgt ttagccgttt gcgcagcgat
     7381 ccgtccaaaa tcttttggtg ttacttcaaa acggatcgta tcaccgatct catatgctgg
     7441 attgattaaa agtgcatctt tcaatgagac ttccaactgt gaatccatca cttcttccgt
     7501 tacttctttt accgcataga catggatctt tccttttttc tgatcaaatt ccacttcgac
     7561 gttttgtgct tggccatagt gacgtttgta tgcagaaacc aatgctgctt ctaaagcatc
     7621 gatcacgatt tcttttgaga tccctttttc agcttctaaa gcgtctaacg catttaacat
     7681 ttctttactc atttttttct gattcctctc cgtgattaaa attggatagc taagcgagct
     7741 tttgcaatat tttttcggtc aaaagtcaat tctttttctc gtgtttttat gcgtattttc
     7801 aatgtaagct gttcagcatc aaatgattga agaaatcctt catattgttt ttcgccatct
     7861 actggttggt ataaggagac atggacataa tcaccaagtg ccttttcata gtcgctttct
     7921 tttttcaatg gccgctctgc tcccggggag gatacctcca ggaaataagc ttgaggaatc
     7981 ggatctggat ctgctgcatc aagtttttca ctcagtttct cactgacaaa ggcgcattct
     8041 tcgatatcga taccgccttc tttgtcaata aatactcgta agaaccagct ttttccttct
     8101 tttacaaatt cgacttcgac aagttcaaaa ttttgttcat ctaaaatcgg tgtcaccatt
     8161 tctgtgactg tttcaacgac gctactcaaa agtttcgcct ccttaaaaat taagtgcgta
     8221 attccattaa aaagagtgag cgaaaaatcc gctcactcac agttagtatc attacctata
     8281 agaactatac catactctgc ttaaaattac aagcagacag gaaaacagat gcttatagca
     8341 tatcgaataa cgataattga ttttcatctg gtagatcttt caatacgcca ttgtcgttca
     8401 tatattcgat caatgttttt gaaactttac cgcgggtggc aagatcttct ttggataaaa
     8461 atggaccgtc ttttcttgct tcaacgattt gtttcgccac gttggttccc aaacttggga
     8521 cagctctaaa tggtgcgatc aacgtatcac cttcaataac aaagttaacg gcatccgatt
     8581 tatacaaatc aatcattcca aatttgaatc ctcgttccag catttcattg gctaactcca
     8641 gcactgtcag ttgattcttt tcttttacgg aagcatctaa tcccttgtcc gtgatttctt
     8701 tcattgcttg tttcacagca tcttttcctt tgcacatgga gactagatca aagtcgtctg
     8761 cacgtacaga aaagtatgca cagtaataca aaatagggaa ataaaccttg aaataagcga
     8821 cacgaagagc catcaatacg taagctgcag catgggcttt cggaaacata tatttgattt
     8881 ttgaacaaga atcgatatac cagtcgggca cattattttc tttcatcgcc gatagatagg
     8941 tttctcgcaa ttcatcaggg atcttgttcc attgtccttt acgaacagtt tccatgattt
     9001 taaatgccat cccgctatct aatccagcat ggatcaaata aaccataata tcatcacgac
     9061 agccgattac ttccgccagt gtcgcatctc ctcgtcgaat caattcttcc gcatttccta
     9121 accatacgtc tgtaccatga gatagtccgg aaatttgcag tagttctgca aaagttgtag
     9181 gatgcgtttc ttctaacatc cctcgaacaa aacgagtgcc gaattctgga atacctaatg
     9241 tccctgtttt ggaataaatt tgttcctgac ttactccaag tacttcagga ccggcaaata
     9301 tccgcatcac ttccgggtca tccgtaggga tcgtttgtgg gtcgatccct gataaatctt
     9361 gaagcatccg aataacagtt gggtcatcat gccctagaat atctaatttc aatacattat
     9421 cgtgaatcga gtggaaatca aaatgagtcg ttttccattc tgaattttga tcgtctgctg
     9481 gatattggat cggcgtaaaa tcatagacat ccatataatc gggaataacg atgatccccc
     9541 ctggatgctg gccggttgtc cgtttgactc cagtagcgcc ttttgctaat ctgtcgactt
     9601 ctgcacttcg aaactgcaga ttattgtccc tttcgtatcc ttttacatag ccatatgctg
     9661 ttttgtcggc gactgtacca atcgttccgg cacggtaaac atagtcttcg ccaaacaata
     9721 ctttcgtata gttatgggct tctgcttgat aatcacctga gaagttcaaa tcaatatcgg
     9781 gcactttgtc tccatggaat cctaaaaatg tctcaaatgg aatatcatga ccgtctttgt
     9841 ttaagcgtgc accacatttt ggacattgtt tttctggcat gtcaaatcca gaaccatacg
     9901 taccatcttc aaaaaactcg gaatattggc actctggaca ataataatga ggggctaatg
     9961 gattaacttc agtgattcct gtcatcgtcg caacaaagct agagccaaca gagccacgtg
    10021 aacctactag atagccatct tcattacttt tatgcactag tttttgggcg atcagataaa
    10081 taaccgagaa gccattacca ttaatagaat tcaactcttt ttctaatcgt ttttgaatga
    10141 tctcaggtaa cggatctccg tacatttgtt ttgcttttgt gtaactcaat tctgatattt
    10201 catcttctga acctggaatc ttcggtgtat aaagttcatc tttgactgga atcacttctt
    10261 cgcagatatc tgcgatttta ttcgtatttt ctactacgat ctctttcgct gtttcttccc
    10321 ctaaaaagtg aaatgcagtt agcatctcat cggttgttcg gaaatggaca tccggcaaac
    10381 tatgacgatt caacggattc gctccgccca tagaattgat caaaattttt cggtaaatgg
    10441 catcttcttc gttcagataa tgtacattac ctgttgcaac aacgatttta tccaaacttt
    10501 ttccgatttc taccaaattt tggataattt cttccaaatc atgttcattt tttaccaatt
    10561 cttgctcgat caaaggcgca tagaccgctt ttggcataac ttcaatataa tcatagaatt
    10621 ttgctcggtt tcttgcctct tctacccctt tttgcatcat ggcttcgaat atttctccct
    10681 tatcacaagc tgagccgatc aatagatttt cacgcatctt tttcaattgg gagcgtggta
    10741 ttcgtgggac gcgttcgaag tattcgacat tcgacattga aatcagtttg aacaaatctt
    10801 tcagtcctgc ctgatttttt gctaaaatcg ttacatggaa aggtcgagcc cttttgtaag
    10861 aatctccttc tccgatatgc tcatttagct gatcatgata aagcatgtta tgattgtcca
    10921 ttgcctcttt cacaaagatc catgctaagt gaccagttgc ttcagcatca tagattgctc
    10981 ggtgatgctg ttccaagcta acaccaaact ttttagataa cacacctaat ccgaaacgtt
    11041 tgaattgagg atacagatat ctagctaatt ccaacgtatc aatgactgga ttggccgctt
    11101 caggaatacc ataccttgcg tagctagtat tcaagaaccc catatcaaaa gccgcgttgt
    11161 gggctaccaa aattgtatct tttgaaaatt caaggaacat ccgcaatact tcttcttccg
    11221 actttgaccc tcgaaccatt tcatccgtga taccagtcag atctaccgtt gttcgtgaca
    11281 atggatgtcc tggatcgata aattcatcaa agctttcgat cacatttcct ttatacattt
    11341 tgactgcggc tagctcgatg atcgtgtcat atactgcaga caatcctgtc gtttccacgt
    11401 caaatacgac gtatgtggct tcactcaatg cttcatgtgc atcattataa gcaattggga
    11461 caccatcatc tacgacattc gcttccacgc cgtataagat tttcacacct gctttttttc
    11521 cagcactatg tgcttccgga aaggcttgcg caccaccatg gtctgtaatg gcgatagctc
    11581 ggtgtcccca ttttcctgct tgtgccacaa gatcagagat gctgtttgtt gcatccatcg
    11641 tactcatatt gctatgaaca tgaagctcta ctcgtttttc cccttcagga gcataatctt
    11701 tgcgaggtgt atgtttgact tctatgatat cttgcgcatt catgaccaaa tcacggacaa
    11761 acgtatcttc ttgaatactt cctcgtacct ttaaccagct accgacactg atcgcatcaa
    11821 atacttgttc atctttttcg ccgttcgaga attttttgac gataaatgat gaagtataat
    11881 ctgtgatttt caaagtcaag atcttgcgtt ttgaacgtag ttctctgact tctttgtcga
    11941 atacgtaccc ctcaagtgtg actcttcgtt cttcttcaac gatactgatc attggtgtcg
    12001 ttggttcatc ggctggaata tttcgtccta gctggatggg tccttcaaga gctggcgatt
    12061 gctcttttct ttcctttttc ttttgttcgt ggacaactag actttcagct gcttgcttca
    12121 taaaggcttc tgcctgttct tgtttgcgtt cttcaaaaag tttcaataca cgttccgctt
    12181 gctgctcatc cacttctggt tcgatacgaa attttgggaa tccgtaactg acgaacaact
    12241 cttctactaa tggcaagtat tgttgcttca aataagaaat agctcctgta ctgtcgattg
    12301 gcaagatgac tttacgatct tttatcacag gtacctgcgt tttcaatact tgctgtgcta
    12361 gtggtgtatc gcattgctga ttttccaaag cttgggccca gtaatcttgc aagagctgtt
    12421 catcgaatgt ctggtcatct gctaagatat tcagtttgac ttgtgcaata tctttaaaag
    12481 cgatagtcag atgctgagtc aatgaccgat atagcataat aggcaaaatt ttggaaaaat
    12541 gtagcgtaaa ttcccataaa cgcgatttac gatggaccac tacactatcc atttcaccag
    12601 cagtaagcag tggatgattt ttttctgctt cctctagttg gatttgttcc aacagttttt
    12661 caaataaatc tcgtttttct gacaaagata tacgcccttt ctgatgagaa acaatgcaat
    12721 tgatcgcatc ttatcctctt ctttttcagt accttttagt atactcgttt tttctgagtt
    12781 ttacattaaa aaattggaac tgcgattcaa atcgcagttc caattttaaa agcgaatcat
    12841 cagattgtca aaaacagctg attcagctat ttttattttc tgtgttttaa cgcttattat
    12901 tcgccttggt tcaagaggat tggtaaagta ttcgccaatt cttctttacg tacttcaacc
    12961 atttcgccag ttttcttgat tttcacttct acgattccat ccactgcttt tttaccgaca
    13021 gtgattctga ttggacagcc aatcaaatca gaatccgcga atttcactcc tgcgcgttca
    13081 tttctgtcat caaccagtac ttcatagcca gcttttgtca tcgcttcttc tagttcattc
    13141 gtcaggcttg tttggtattc gtcttttaga ttcatttgga cgatatgcag atcaaatggc
    13201 gcaattcctt ttggccaatt gattccttgt tcatctgaat gttgttctac gattgctgat
    13261 aataaacgac ttacaccgat accataacag cccataatca caaatttttc tcgtccattt
    13321 tcgtctagta cagttgctcc catagattca ctgtaacgag tgccgatttt gaatatatgt
    13381 ccgatttcga ttcctcgagt aaaggcaaga acaccgttgt tgtctggtga aggatctcct
    13441 tcttgtacaa aacgaagatc ttcataagaa agcacagtga aatcgcgttt tggattcaca
    13501 tttttatagt ggaatcctgt ttcgttcgct ccagtaatgg cattcgctaa atcttggaca
    13561 taacggtctg catatacttt tacgtcatct gaaacaccga ctggtccgac agaaccaaac
    13621 tcagcaccta gatatttttg tgcttcttct tctgttgctt cttctaaaaa gtcagcagat
    13681 aaaaagtttt tcagttttac gtcattgact tcatggtctc cacgtaccaa aacaaggaca
    13741 ggttgttcat ccgcaatgaa caatacagac ttgatgattt tttgaggttc aacagaaaag
    13801 aaatctgcta cttcgtcaat tgattttacg ttaggtgtag ctactttctc caattcaagc
    13861 tgtgtttcat gcgatttttt tgatacatag tagcttgtag ccatctccaa gtttgcagca
    13921 taatcacttt cagtcgaata acagatcgta tcctccccga tttctgagat cgccatgaat
    13981 tcctttgagt ctttcccgcc cattgcgccg ccgtctccga tgatcgagcg aaattctaag
    14041 ccacaacgct tgaatacttc ggtataggct ttttcgtaat ctttatatgt ctcgtccaaa
    14101 ctttcttccg ttgcatggaa agaatatgca tctttcatga taaactctct tccgcgaagc
    14161 aaaccgaagc gaggtcgttt ttcgtcacga tatttaggtt ggatttgata aagatttaaa
    14221 ggtaatttct tatatgaatt gatttcatta cgcatcaaat cagtaaaagt ttcttcgtga
    14281 gttggtccaa ggatcatttt tcgatcattg cggtctttga aacgatacaa gtttggtcca
    14341 tatgtttcgt aacgcccgga ttcttcccaa agttctgctg gcaagatggc aggcatcagc
    14401 atttctaccg ctccgatttt ttcaaattct tcccgcataa tgcgtttcag tttttccaat
    14461 acacgatttg ctaatggcag atatgaataa atacccgctg atacttggcg gatatatcca
    14521 gctcttagca agatttgatg gcttaatact tctgcatcat ttggaacttc gcgtaatgtt
    14581 ggaatcaaca tttttgattg tctcattcta tatactcctt tatttcctca aaatagttat
    14641 cgtttgttat tataccctat ttgtcaactt tttctagaaa aagaatcgtt gaatatcgtt
    14701 ccatgtaact agcaccatca acaacattaa aaatccaaag ccagctaatg tcaatattcc
    14761 ttctttttct tgacttaatg gtttcccgcg aatcccttca aaaatattca agactaattt
    14821 cccgccatct aaggcaggaa tcggaagcag attgacgatt ccgagattca ttgaaagaag
    14881 cgccatcaaa ctaataacag ttgttacacc ttgatttgct gcttctgaag acaattggta
    14941 catcatgaca ggtccgccta atttatctaa actgaaacct gtaaatagtg atcctaaggc
    15001 tttgaaaatt tccaatgaac cgctgaaagc ttgacgcgtc cctccaatga tcttatccat
    15061 aaaacctgta ttcattgggg cttttatacc aagttggcca actttttcgc catttgactc
    15121 tacagatttc ggtgtcacgt cgacagattg cacctgacct tctcgttcga tcttaaagtc
    15181 taatgtttta cctggatttt tcgtaatgac tgttgtcaga tcattccatg aatggatttc
    15241 ttttccgtcc acactgacta cctcatcatt ttcttttaag cctgcttctg ccgcagctcc
    15301 attaggcatg atctctccta cgcgattagt gttcgtcact tgaacaccac cttgcataaa
    15361 tgccagaatg atgaataaca cgattgccaa aataaagtta ttcatgggtc cagcaaagtt
    15421 ggtcagcata cgctgccaca atttagccga ttggaactgt acgtcttttg gcgcaattct
    15481 gatttctgtt ccgtcgtttt caataatcgt cgcatcgtga tcaacagggt aggttactac
    15541 ttctgtttcg tctccattca cataaccagt gatagtcagc tcatcttcta aatcataacg
    15601 gctcaattcc atcgggatag catttgtcaa ttggattttc ttacttaaat ttatcttctc
    15661 tacgatgccg tcagaattta gtaaaagaga aagaggcatt cctggtgcca tttctgtttc
    15721 atcatcccca ttgcctgcca ttcgaacata accgccaata ggtaataggc gaagcgtata
    15781 tgttgtcccg tcttttgctt gatgcccata aattttcgga cccataccga tagcaaattc
    15841 gcggacgagg attcctgatc gtttcgcaaa gaagaaatga ccaaactcat gaacaatcac
    15901 taatatacca aaaacgatga taaatgtcag aatcgttttc atagcttttt tctcctttac
    15961 ttgttttttg tgttttttat aaaacaacga taaaacacta taattttacc atagtatacc
    16021 aaagacacca atagtatcaa tataatgaaa aataaaaacc tgcgtctttt atgaaattca
    16081 caaagaaacg caggtatact tactaaataa tgccaaataa atgcatgata ggaaatacaa
    16141 acagcaaact gtcgaaacgg tctaagattc caccatggcc tggtaagata tttcccgagt
    16201 ctttcacatc ataatgacgt ttgattgcag attcgaccaa atcaccaaat tgtccgactg
    16261 ctgaaaaaat aatcgtcatc agaagcatga caaacatacc atgattgaat agttcttttg
    16321 ctgggtatag caataaataa agaaatgcga ctacaacagc actaccgata ccaccgagcg
    16381 caccttcaat cgttttgttg ggcgaaatat caggacttaa cttatgctga ccataacgtc
    16441 gaccaaccat atatgctccg atatcagttg cccaaacaat aaatagacca aatatcaaaa
    16501 cagccaagct ttctgttcgt gcattgacaa aattctggaa acctattccc acatacaagc
    16561 tgacaagcac aggaaaacct gcttcatcaa tcgtatacgt atttttagaa ataactgatg
    16621 ttcctaaaag aatcataact ataagataaa ataacataaa tgtacttaca ttctctggaa
    16681 gaaagaaaaa ccatctttct tttggcaaaa caagtaacac tgcaccaacg gcggataaaa
    16741 ctccttcaaa acttaagagt gtcaatcctt tcatccggaa cagctcgtaa actcctacta
    16801 ccgctaagat tgctgctgta agctcaaccg ctatgcctcc gatccatata atcggaataa
    16861 acaagactag cgccacagct gctgtgatca cacgttgttt catgactgat cccctttctc
    16921 atcttctgtt ttttttacac cgccgaatcg acggtctcga ttttgataag aggcgattgc
    16981 ttcttcaaga tgcgcaccat caaaatcagg ccaaagcgct tttgtaaaat acagctcact
    17041 atatgcgatc tgccataaca gaaaattact aattcgctct tctccacttg tacggatcat
    17101 cagctcagga tcttgtaact ctttaggcaa gaaacctgtc attaaatgct tagcaatcaa
    17161 ttcatcatcg atttcttctg cgtgtatttc tccttttgct acttcttcgg caatttcttg
    17221 gacagcagtc acgatctctg ctctacttcc ataattcaaa gcaaaattca ataccattcc
    17281 agtattgttt tttgtttgct caattgcacg tcttacagca tcttgcgtat gttctggcaa
    17341 tacgttttca taacccatga catgaacttt gacattttct ttaatcaatt ctggtacgaa
    17401 cgtatcaaag aagtcgacag gtagttgcat gagaaaactt acttcatctt ttggtcgctt
    17461 ccagttttca gtcgaaaagg cataaagcgt caacactttc actccaagcc ttgatgcttt
    17521 ttttgtgact ttctttactg tttccatgcc ttctttatgg ccagcaactc gtggcaaacg
    17581 acggttttgc gcccaacgac cattgccgtc catgatgatg gcaatgtgct gaggaattgg
    17641 tccatcggta ttaaattgaa attcgctttt ttcttgaata tatttgtttt tttgcggaaa
    17701 aaaacgtaac attctttttc ctcctatact taaaagattc ccactatctt aagtattata
    17761 gcaatatcta actgtttttc ctatgaaatc attgtttttt tatttaaaaa ctgaataatt
    17821 ttttcttttt ttaattttta aataaaagaa atataaataa aaacagagga agcggaatga
    17881 tccgcccctc tgtttttatg attttattaa acttccaata attctttttc tttatcagca
    17941 gcgatcttat ctacttcttt aatgctgtta tctgtcaatg tttgtacgtc tttttctaat
    18001 ccgcgcaaat catcttcggt aatatcacca tttttttgtt gttttttata ttcatcgatc
    18061 gcatcacgac ggatgttacg aatcgcgatt tttgcatttt ctgcttcttt tttcacatct
    18121 ttagctaatt cttttctgcg ttcttctgtc agttgcggga taactaagcg aatgacattt
    18181 ccatcattgg ttggactgat tccgatatca cttgccataa tagctttttc aacatcttga
    18241 agaatgctct tgtcaaaagg tgtgatcatt aaaacacgtg cttctggaat ttggattgat
    18301 gccatttggt tcaatggtgt tggcgcaccg tagtagttca cagtaatacg gtccaaaagg
    18361 ctagcgttcg cacggcctgc gcggatttgc ccaagttcac gttgaagatt ttgtgctgct
    18421 tttttcattt tttcttttgc ttctgtcata attgcatctg ccatttttat ttccccctta
    18481 cagttgttcc gatattttcg cctaagattg cacggcgaat atttccgtgt tcatttaaat
    18541 tgaagacaag cagtggaata tcattatcca tgcttagtga acttgcagtt gaatccatga
    18601 cttgtaatcc tttggcaata acttccagat gagtcagttc ttcaaatttt actgcatttg
    18661 catctacttt cggatcagca gaataaacac cgtctacatt gtttttagcc atcaggataa
    18721 catctgcgcc gatttctgcc gctcgtaatg ctgctgttgt gtctgtcgag aagtacgggt
    18781 tgccggtacc gcctgcaaag ataacgatcc gtcctttttc taaatgacgt tctgctttac
    18841 ggcgaatata tggctctgcg atttgacgca tttcaatcga agtttgcacg cgtgtcggta
    18901 cacccacgtt ttccaacgta tcctgtaaag ctaaggcatt cataactgta gctaacatac
    18961 ccatgtaatc agcttgagca cgttccatgc ccatttgtgc tccgatttgt ccgcgccaga
    19021 tatttccgcc acccacaacg attgccattt caacgcccag ctcgtgaact tctttgattt
    19081 cttggatgat ttctttgatt actggtggtt tgattccaaa tcctgcatcc ccagctaaag
    19141 cttctccact caattttaag actacacgct gatatttcgg tgtgaccatg ttcattcctc
    19201 caatattttc tattgccatt ttaccatact tagatgattt cgacactcta agactggttt
    19261 ttcttgtttt gcttaaaaaa agggagcgca caaaccgtgc gttcccgtct gttattcaac
    19321 agaattattt tttcacttgg ctcataactt cttctacaaa gttatcttcg cgtttttcga
    19381 taccttcgcc aacttcgaaa cgtacgaatg ttttaacagt tgcgcctttt gatgcaacgt
    19441 atttttcaac agtcatatca ggatctttaa cgaatggttg gtccaccaaa gcgatttctg
    19501 ctttgaattt cttcaagcgt ccttctacca ttttttcaac gatattcgct ggtttgcctt
    19561 cgttcaatgc ttgttctgta agaacagttt tttcatgttc taattcagct tctggaattt
    19621 gtgattcgtt tacataacga ggattgatag ctgctacgtg catagctacg tctcttgcaa
    19681 ctgtttcgtc agttgttcca tctagaacag tcaatacggc aatacgtccg cccatatgca
    19741 agtatccacc aaatgcagca ttgtcgtctt tttctacaac ttcaaagcga cggaagctga
    19801 ttttttctcc gataacttga gttgcttcga tcaagtcaga ttcaattgta cctttatctg
    19861 ttttgatttt cattgcagct tccatgtctg ctggtttgtt ttcagcaaca agttctgcaa
    19921 tttcttttac taaatcttgg aacatttcgt ttttagaaac aaagtctgtt tctgagttta
    19981 cttcaacgat tgctgcagtg ttacctttca cagcaactga agccaatcct tcagctgcaa
    20041 tgcggtcatt tttcttcgca gcctttgcca ttcctttttc acgtaaaaga tcaactgctt
    20101 tttccatgtc gccttctact tcaactaatg cttttttcgc atccatcatt cctacgccag
    20161 tcatttcgcg tagttctttt accattttag ctgtaacgtc tgccatgttt aactcctcct
    20221 gtgtgttttt ctttttaaaa aaagctgctt caaaggagag tgaggcttgt cgcccagcct
    20281 ttgaaacagc tccatcattc aaaattattc tgctgaagaa ttgtcgcctt caacaacatc
    20341 tacgatttct tcgattgatg ttgctgtgtt ttcttctgtg aaatcttctt caactacttg
    20401 atcttcacct tggtttcctt cgatgaaagc atcagccatt tttgaagtaa tcaatttaac
    20461 ggcacgaatt gcatcgtcgt ttgaagggat tactacgtcg atttcatctg gatcgcagtt
    20521 tgtatcaacc atagctacga ttgggatatt taatttgtgt gcttcttgaa cagcaatacg
    20581 ttctttacgt gggtcaacga tgtacattac atctgggatt cttggcatat cagcgatacc
    20641 gcctaagaat ttttcaagac gttcacgttc tttgttcaaa ccagcaactt cttttttagg
    20701 tagaacttca aaagttccat cttcttccat tgcattgatt tgtttcaaac ggctgatacg
    20761 tttttggata gtatcccagt ttgtcaatgt tccgcctaac caacgatggt taacgaagta
    20821 ttgtcctgca cgagttgctt cttctttgat tgcttcttgt gcttgttttt ttgtacctac
    20881 gaataatgct acgccgcctt cttcagcaac gctcttcatg taatcataag ctgcatctac
    20941 taatttaact gttttttgca agtcaatgat gtagattccg tttctttctg tgaagatata
    21001 tttcttcatt tttggattcc agcgacgagt ttggtgacca aagtgtacgc cggcttctag
    21061 taattgtttc atagaaatta ctgccatgtt tgtttcctcc gatttggttt tatttttcct
    21121 cttcttatct tctaccttgc cggcaaactt tttcaagcac ctaccgacaa tccaataaga
    21181 atgtggattt gctgatgcat aaagcaccct cagctattat acagacaaaa gatccgtatc
    21241 gcaagagcaa atatactttt ctgctcaaca ttcaatagtt tacctgaaag ataaaaagaa
    21301 ttcaagaaaa acagtagatt tctctttgtt tctttattat cctttttcca taaaataatt
    21361 ttaggtataa aagacgaata ttattgggca atccttcttt tttttgttat gatacatgtg
    21421 aaatctttcg taaaggagaa agaaaatgcg caaagctcaa agacatttgc tgattaaaca
    21481 agtcattgaa gaattcccga tccgtacgca agaagaacta ttaaacaaac ttgggcatta
    21541 tggcgttacc gccacgcagg caacgatttc tcgagatatc cgtgatctaa aaatcgtgaa
    21601 ggcacctgat gaaaatggcg tttcccgctt cgtattgttt cgcgaaaaag gaactcctga
    21661 gactaggact gaggaagagg atcggctggt tcaaatgatt gaagacattg tattgaaagt
    21721 cgaacgagtc cactttttga cgatcgtcaa taccttgcca gacaatgctc atttatttgc
    21781 atctgtttta gatgagatca aacctccctc tattgtcagt acaattgctg gattcgatac
    21841 gatcatggtc atctcggaaa atgaggagga cgcaaaacga gtagaagaat ttcttcacca
    21901 tccaactgaa gtatcacttt tttaatcaga gcacgtattc ctaatatgga atgcgtgttt
    21961 ttttattttc aatattttcc ttattaaata aacctttaca ctctttacaa aaatacatca
    22021 ttttttattc aattttttca acaccttgat tgaatcacaa taatcatcat ttatattgga
    22081 tgttaacaag agcagcttct tgctctttgt gtatttcaaa aaagaaatga aaggaaatca
    22141 tatgtataaa ttattaactc ctggaccatt gacgacttct gaaacagtga aaaatgctat
    22201 gctgatcgat cattgtactt gggatcaaga atacaaagaa attacacaat ggataagaaa
    22261 aaaattattg gatcttgctc aagtatcgga aggaatatac acaacggtct taatgcaagg
    22321 tagcggctcc tttggggtag aatcggtatt aagttctgtg atcaggccag aagatactgt
    22381 actgatttgt tcaaacggcg catatggaca acgaatgatc aacatgtgcg aatgtctgaa
    22441 actgaactat accgtttatg aggtatcgga acatcaaatc ctccaagctc atgtgattac
    22501 acagctgatt gaagagaatc cctctatctc cgcactagca atggtccata gcgagactac
    22561 ttccggtatt ttgaataatt tggaagagat tgctgctgtc acacaagaaa aagatctttt
    22621 gtttatagtg gacgcgatgt caagctttgg tgggataccg attcgtgtag ccgaactagg
    22681 gattgatttt ttgatttcca gctcaaacaa atgtattcaa ggtgttccag gcttttcttt
    22741 tgtacttgct aataaagcgg aactagaaaa gcgaaaaaat gtgagtcgaa gtttgtcact
    22801 tgatctatat gaccagtacc gcgtgatgga aaacgagaat ggcaaatggc gtttcacttc
    22861 ccctacccat acggttttgg cttttcaaca agcgctgaag gaactagagg cagaaggagg
    22921 aatcgaaact cgatattcac gttatctcgc caataatcat gtactacgac aaaaaatgac
    22981 agagatgggg ttccagcctt tcattcaaga atatcagggg ccattcatca cctcttttct
    23041 ttaccctaag gactggcgat ttgattttaa tcatttttat cagtatttaa agcaaaacgg
    23101 atttgtgatc tatcccggga aaatcagcga tcatgaatgt tttcgtatcg gaaatattgg
    23161 cgagatctat ccggaagata ttttggaact gacagcaatt atcaaagact ataaggagaa
    23221 cgttaatgat taaaactgta atttttgatt gggcaggaac aactgtggac tttggctgta
    23281 tggctcctgt ccacgctttt agaaatgctt ttctagaaaa aggaatccaa ctgactgaca
    23341 aagaaatccg cgaaccgatg ggaaaattaa aatgggatca tatccaacag cttctttccc
    23401 tcccttctgt caaaaaacaa tggatagcta tttatggaca actacctcag gaaaaagagg
    23461 tggaggaatt gtatcaacaa tttgaacatt atctgtttaa agatttggtc aagcattcta
    23521 ccttgaaacc cgacacattg gaaacgatcg aacggttgag aaacgctggg atcaatattg
    23581 gttccacgac tggatttact gctcctatga tgacaatcgt agcagaaacc gcaaaaaaag
    23641 aagggtattc accagacttc atggcaactt ctgaagacgt cgaagggtat ggtcgaccgt
    23701 ttccttatat gatccataaa aacatgcagt acttcaaaaa caagtcggtt gatgaagtaa
    23761 tcaaagtcgg agatacggtc tcagatatcc aggaagggaa aaatgccgga gtgttgacag
    23821 taggtgtaat tgaaggtagt tcgctaatgg ggatttctta tgatgagtac caaaaattag
    23881 agatggggaa aaaagatcgt ttaaaacagt ctgttgctct tagattccaa caagctggcg
    23941 ctgatatttg tgtaaacaat ttaaaagagt taacagaata cattttgtac gctgacaaga
    24001 atccagaatg acttttatac aaaatcagag gtcgtgaaaa agctgtactg catcaagtaa
    24061 taagaacagc caaacaaaaa tagctcctca tatttttcgt ttggttaggc ttattaccgc
    24121 agatgcaagc ttttgaacac cgtttattcg aaaaaaaaag ggagctgtga cacgactttt
    24181 gtcacagtcc ccttatttac aaacaaacag tgtttaagag acaacctctc cactaaacgg
    24241 tgttcattca gctgactctc ggtatttgct caaaaaacga taaaatttga aaagctattt
    24301 tctcttttgt ttctctaata ctcggatcag aacgctgatt tcacattctc tttttcttac
    24361 atcttatctg gtgcatgaag tcctagcaaa cgaaggtctt ctttcaataa tacagtcact
    24421 gcataaacta gagctaagcg tgcttctttt tgttcatcat cagctaaaat cttcgtatga
    24481 gcatagtatt tgttgaatgc ttgtgctaat ttgattgcat gtttcgcaat cactgacggt
    24541 tcgtattttt ccccagcaga caagaccgtt tcagggtatt tttgaactaa tttaaccact
    24601 tcccagctgg tatcgtcatt caatgcatag tctgctgttt ctgaagggac aaatcctgct
    24661 ttttccaaca ggctgactgc acgagcatgt gtatattgca cataaggtcc agtttctcct
    24721 tcaaatcgaa caacttcttc caggttaaag tcaaatgtat tcagtcgatc gtttttcaaa
    24781 tcgtggaaaa tgactgcccc aaccccaact tgtttagcta cagcatcttt gttttccaaa
    24841 tcagggtttt tctcgctgat ttgttcttta gcagattgga ttgcttcatt taatacttct
    24901 tctaataaga cgatctttcc tttacgagtt gataattttt taccaccttg cgtgatcaaa
    24961 ccaaatggaa tatggcgcat atcgtcagac cagtcaaagc ccatttcctt caagacagct
    25021 ttcaactgtt taaaatgata gctttgttca ttccctacta catatagaga ttgtttgaag
    25081 tcgtaagtac gtttacggta caatgcagca gctaagtcac gtgtaatgta tagtgtcgca
    25141 ccgtctgact tcttaatcaa cgctggattc aggtcataag cagataggtc aacgatctct
    25201 gcacctttat cctcgttcag caaatgtttt tcttctaaaa gtttgacgat ttcatccatc
    25261 ttgtcattgt agaaagcttc accgttcaac gaatcgaaac gtacttccag cagatcatag
    25321 attttattga attctttcat cgattcatca cggaaccatt gccataattg aatagcctct
    25381 tcatcgcctt cttccagacg tttgaaccaa gcacgagctt cttcatttaa ttctggttct
    25441 gtttcagcta cttcatggaa ttggacatat aaacgaagca attcattgat tggttctgct
    25501 ttaacagctt cttctgtacc ccattttttg taagcgacga tcagttttcc aaactgtgtt
    25561 ccccagtcac ctaaatggtt gatgcggatt ggctgataac cgattttttc cataatgaaa
    25621 ccgatcgaat taccgataac tgtcgaacgc aaatgtccca ttgagattgg tttggcaata
    25681 ttaggagaag acatgtcgat tggtactgtt ccttgattcc cgatattgct atccccataa
    25741 tgttcttttt ctttcactac tgtttgcaaa acttttttgc tgataagttc tttattcatg
    25801 aaaaagttca agtaaggtcc gacaacttcg attttttcaa agttggcaga atcgattttt
    25861 tctgctaaat ctgcagcgat ttgctgtggc gcttttcggt aaacttttgc taatgaaaag
    25921 gctggaaatg caacatctcc gtgttcagct gattttggat tttctagtaa ttgttcgact
    25981 tgttccagtg ttaaatcatc tttgacagcg tcatagacag cttttgcgac aagatcttta
    26041 ttattcatct ttattcctcc taaagtgctc ccttgattgt taaacaaaaa aaatctctag
    26101 cgacggcata cttagtatgc catcactaga gacgagattt cccgcggtac cactccaatt
    26161 gtcctaaaaa aggaccagct ccatctgata tcggtagacg accgtctgtt taaaacagaa
    26221 ttctccaagt gcgcttcgtt gtatcttctt cattcggctt ccaccacttc cgaattcgct
    26281 agccaagaat gatacaagta ctctcttgtt caacaaatct tagtcttatt aaactttctt
    26341 gaattatatc agaaaaaaaa acgaaaggct agtcgtttct gataaaaaaa caatccagat
    26401 ggcctaaagg agaagaaata ccacctggat agctataaat catttcgtaa atacgaaaga
    26461 cttaacaaat taatcagcta cgataatcgt tccttcttca gaagctaaaa gattctcgat
    26521 attttctaaa gaagtgatga ttgccttagc gtttggtttt gcttcaacaa atgcaatagc
    26581 tgcctctact ttcggaagca tgcttcctgg agcaaattgc ttttcatcaa tgtattgttt
    26641 catttctgaa acagtaacag tttctaattt cttttgatct ggtttttggt aattgacata
    26701 cacattgtct actcctgtca atacgatcag caaatccgca tcgatgattt cggctaattt
    26761 ttctgaagca aaatctttat cgattacggc ttctcgtcct tctaatccag tagctgtttc
    26821 tacaacagga atcccaccgc caccaacaga gactgtgata actccttgat cgaccagcgt
    26881 ttcgatgaca cgtgcttctt taatcgagat gggtttaggg gaagcgacta cttttctcca
    26941 tccacgtcca gcatcttctt tgaatgtcac agtggagtca gcattcattt gttctttggc
    27001 ttcttcttcc gtgtagaatg gaccgactgg tttacttgga tttttgaatg aaggatcatt
    27061 ttcatccaca ataacttgag tgactaaaga aaccacatct ttatcgatcc ctttttcctt
    27121 cagtacttcg cccatggcat tttgtaacca gtaaccgata gatccttctg tcattgcaac
    27181 acatgtatct aaaggcattg ccggtgtttt ctcagaatca gcagcttgct gctgaatcaa
    27241 taggttaccg acttgcggac catttccgtg ggagatgatc aattcatctc cttgttcgat
    27301 aaatttcact aaatactttg ctgtttccat taacgcttct tgttgggctt ttgcacttgc
    27361 atccgtagac aagattgcat taccccctaa agcgacaact acttttcgtt tggccatttc
    27421 attcgtcctt tcttcagttc taccgaattt tctagataac aaaacgagcc agcgttttgt
    27481 gattttccct caatgctgac tcgttttttc gtgagatgaa tgtggttatg cttaaacgcg
    27541 agggataaac aagttaccta atgttgctgc catgatcgct ttgattgagt gcatacgatt
    27601 ttctgcttgg tcaaattgac gagcatattt gctgcggaag acttcatctg ttacttccat
    27661 ttcagtgatg ccaaagcgtt ctttcatttg ttcaccgtaa acagtgtttg tatcatggaa
    27721 agctggcaag cagtgcaaga agatcaaacg atctgtgttg tgtgtttttt ctaccatttc
    27781 catattgatt tggtaaggtt tcaacaattt gatacgttct tcgaatttat cttcttcacc
    27841 cattgatacc catacatcag aataaaggac gtctgctccg tctacacctt tatcaacatc
    27901 gtcggtaatc atcaattgtg caccggattt ttcagcaaag ccttctgcca ttttgacgat
    27961 ttcttcttca ggttgtaatt cttttggtgc aacgatacgc atattcgctc ctaaaattgc
    28021 acctgtgact aataaagagt tagccatatt gtttcgacca tctccgcagt aagcaacagt
    28081 gataccttct actgttccaa agttttcttg gatagttaaa aagtcagcga tcatttgtgt
    28141 tggatgccat tcatcagtca agccattcca aactggaaca ccagagaatt ctgctagttc
    28201 ctctaccatt ttttgactga atccacggaa ttcgattcca tcaaacatac gacctagtac
    28261 tttagctgta tcttctgtgg attctttttt acctaattgg atatcatttg cacctagata
    28321 ttcaggatgt gcacctagat cgatcgctgc agtagtaaat gcagaacgag tacgtgtaga
    28381 tgttttttca aataaaagcg caatattttt tccttctaga tagtgatgtg ggataccacg
    28441 ttttttcaag tctttcaagt gttctgaaaa atcgatcaaa tattgtaatt cttcttttgt
    28501 aaagtctttt tctgctaata agcttcttcc ttggaatact gattctttca tattaaacat
    28561 tctcctttag attgttttta tttaagatta aagaggaaat cccgacggca gctgacagta
    28621 agaccgccgt caggaaacaa attacttcaa gtcttcacgg acaagtggtt ggctcatgca
    28681 gcgtggacca ccacggcctc gtgacaattc acttgaattg atttctaaca ctttgatgcc
    28741 atagctacga agcaattcgt tcgatacata atttcggtta taagtgacta caacacctgg
    28801 agcgattgcc aatgtgtttg atccatcatt ccattgttct cgaggtgcaa cgatctcatc
    28861 tccattacca gttgggatca gtacaagatc atctaatcca agagcagctt tcatcgtttc
    28921 gtgtaagtta tctgaatgag taatcttgat atcatcacca tctggttcga ttgtaaagac
    28981 atccatctta ccgtctttac tttgaattgc tggatggata gtgaatttat cgtgatctac
    29041 cattgtaaat acggtatcca aatgcatcat tgcacggttg tttggaattt tgattgctaa
    29101 cactttttca aatcctgaat tttttgcgaa taggtggcgt gctaattttt ctaaagcttt
    29161 cgcattagtt cgttgagaaa tcccaacagc tactacttta tcgcttaaga tcaattcgtc
    29221 tccgccttcg atatgatctg gattttcacg atccaaccat acttcgattc ctttatttgc
    29281 aaatcttgga tgatgtttca aaatatattc tgtaaacatc gattcacgac gacgtgcttc
    29341 aaaagtcatc ttattgactg tcatcccatt tcccattgat gcggaagggt cgcgtgtaaa
    29401 gtacaagttt ggcattgggt ccatgtaaaa tggatagtca tcgtctgctg acagatcgta
    29461 tagactgcta gaacgtactt caatatcttt tgtacgaacc ccagccatga ttttatctac
    29521 catttcttgc gtttccattg atagaaggaa ctcatgaaga ccctctctta cagcattcga
    29581 tgcaatatga gattcatcca acattttatt caagaattgt tctttgacat tgcctgcgtc
    29641 aatcgcttct gccgctaatt tttccaagta aagagtctcg acgccttcat tttgcaatgt
    29701 ctttgcaaag ttgtcatgtt cttcttgagc aatcggaaga tatggaatat catcgaacaa
    29761 cagacgatcc ataatatctg gtgtcaaatt ttccacttct tgtccaggcc ttttcagtaa
    29821 aactgtcttt aatttcccta tttcagagaa aacgtgaata ggtttatcca tgttgtgttg
    29881 cctccttctt tttgattaca tgtataggat atcctccttt tgtaatcact ttcataattt
    29941 tgtctcattc aaaatgagac attcctcaca ttaatattcg ataattaaat aattaaacgt
    30001 ttttctattt ttaagcgtaa tttattcatt ttttattctg tatacacttt caaaaaaata
    30061 tttattcatt gaaaaaaatt ttttgaataa attaaaaaaa taaattatat tagcgttgtt
    30121 agcgtttact atatgtatta ttgttgtttt ttatacacaa atcattcgct tcaaaacaaa
    30181 ttattcaata ccattgtata ttttttacat aataacaaat tatatttgta aagtattaaa
    30241 acttgtttct aatgttggta tgttaaaact ttacaaattc tttaaaaaaa gtgacaataa
    30301 atatagtgaa caataaggag aggacttttt aatgcgtgtt aataaaatcg attttcactt
    30361 ttcaaaactc agatatcaac ctgattttga acatttctcg gatgaagagt tccaacagat
    30421 caaggaacat actgttcttc gttcttataa aaaaggacag atccttttcg acgatggtga
    30481 tcccagacaa cggttgtata tcttgaccaa aggactcgtt cgtttagaac gttatgatga
    30541 aagtgcaact tattactact atgactatgt gaatacaaat aacctttttc ctatgtcagg
    30601 aatgtttgga gaagagacct acacttatac agctcaagcg atgactgata ttgaaacatt
    30661 ctaccttcct gtttcctact atgaaaaatt agcacgaaaa aatcccgaac agttgatcta
    30721 tatgatacaa aaaatttcta aagtagttga aatacatgaa ttgcgtatcc aaatcggatt
    30781 gacctctagc gcctttgacc gggtaaaaca aaatttattc attttgaaag aagaattggg
    30841 cgtaccaaca aaagatggtc atatcaccat tccttatccg atcaccttaa aagaattagc
    30901 agtgaatagc ggcacgacac gtgaaactgc tggacaagta atcaaacaac tacgtgattc
    30961 cggactgtta gattatgaca aaaagcgttt tactttctgt aagacagcta ctactccagc
    31021 tatgagagaa taacgcgaac aaaaataagc agtgaccctg atatttatca gggtcactgc
    31081 ttattttcct ccctaaaact tctgaaagat ccggcagttt tagatcactg ggcaagactc
    31141 aaagattttc tatgtgtgtt tcagttatgt aatattcact gtttgcaaaa aatactgata
    31201 gattaactag aggaagcgtc ttgtcctcat ccgacctaga ttattcgata ttcctcgaca
    31261 ataaacgatt aatcagtgag ggcaatcaga ggaacggata atgatgaata agctgtaaaa
    31321 aatagggctt tgacaaaagc gtgtcacagc ccttatttcg attaaacggt gtgtacctgt
    31381 caactcttcg gtcacaagtc ataatgttca taaacatgag gagctgtttt ttgaaattgt
    31441 tccttgtgaa tcagagttaa acgacaggct tacgaactct tgattaaacg gcgttcaaaa
    31501 gcttgcatac gcggtaataa gaacagccag gtcaaaaaca tgagaagccg tttttcctgg
    31561 gctgttctta ttacttgatg cagggcagat ttttcacaac ctctttattc agataaaacc
    31621 aagcttggat cagcattcaa ctgatagcta gcaaaatttc ttttttgcat ttccacatgt
    31681 gctgctgctc caatcattgc agcattatct ccacaaagtc gaagaggggg aatcactagt
    31741 tctacctcag ggagtttagt tgccaaagca tcctgcaatc cttcacgcaa cccttgattg
    31801 gccgctacac ctccagctac aatcagttgc ttgactggat aattttgaca agctcgaagt
    31861 gttttagtaa tcaatacatc gatcacactt gcttgaaagc tagctgctaa atcatttttg
    31921 tctagatctt ctccacgctg ttgtgcatta tgcaccaaat tgataaatgc actttttaat
    31981 ccgctaaaac tgaaatcata attatcttca tggatcatcg ctcgtggaaa atggtaatta
    32041 tcttttccct gatgagctag ctgatcgatt tcttttccgc ttgggtaaga aagacctaat
    32101 actcttccaa ctttgtcata agcttctcct gctgcgtcat ctcttgtctc cccaatgatt
    32161 tcatatgatc catcttcttg catatagact aattctgtat gtcctccgct aacaagaagt
    32221 gccatcaatg gaaactggaa tggtttaacc agacgggctg catagatatg ccctgccata
    32281 tggtttacag gaataagcgg caattggtgt gcccaagcaa aagccttagc tgcagaaata
    32341 ccaatcagca acgagccgac aagtcctggt ccataagtga cagcgactgc cgataaatct
    32401 tctgccgaca caccagcttc taccagtgca tcttccaagc ataaggtgat ttgttctacg
    32461 tgatggcggc tagctacttc aggaactacg ccaccaaaac gtttatgact gtttatttga
    32521 gaagcaacga tgttagataa gatctctgtt ccatttctaa cgaccgctac actcgtttca
    32581 tcacaacttg attcaatcgc cagaatcaat tcttcattca tttcatcgtc tccgttttta
    32641 actttctgct cataatgatc gcatcttccg tcggattgcg ataatatgat ttccgtttac
    32701 ctaatatacg aaacttttct gattcataaa gttttcttgc tgcttcatta gatagacgta
    32761 cttctaaaaa aacatgcagc gaatctttta tttgttccgt aatcagtaat tgtaacaaat
    32821 gccgtgcatg cccttttcct tgttctgaaa ctgcaacagc aatatttgtg atctcgactt
    32881 catctattac tttcgagtat ccaataaagc cacagatctg gttatcttca actaaaagca
    32941 gataatcaga gtgaggctgt tggatgtctg ccaaaaactg cattttcgtc caaggtgaac
    33001 catgtggata agcattttcg ctaatcatcc atacgcattc tgccaagtac gcttcatcaa
    33061 aatctttttt tctgcaaatc aactaatctc actcattttc taaaagtttt atcatatcca
    33121 acgcatcttc ttttgtttca gtgtaatatc tttttttgat cgttgttgct tgaaaaccta
    33181 acttccgata aagtctttgt gcatcttgat tactcatacg cacttctaat gacatcgtct
    33241 cacagcgatt cattatagca aagttctcga tttcgtcaat aagcagcgaa ccgattcgtt
    33301 tacgctgata tgccggtcga acggcaatat tcgtgatatg gcaatctaaa ccgaacgctc
    33361 ttgaaccgat aaaaccaatc attcttccat tatcaaagat acctagatat agatgaggaa
    33421 gcggtgactt caattctgat ataaaggcac tttttgtcca tggcaattcg ccattataga
    33481 cttctcgttc gacatccagc agctctttga tatcttctgt agtcaattct cttagtacgt
    33541 aacgacgatc gatttgttcg attgtttttt ctgtataaat cattttatct tgaaaaaagc
    33601 ttctggcata cgctctaaat tttttcaaca taactttcgt ctccaggtgt gtgcgtctcc
    33661 aaccattttt cttctgcttc tacccgtttt aaatagcgag gaagaaaagc ttgtttctct
    33721 gcaactggtt cagctaaagc acctaattga gccacagtta caccacttgg aatatcccat
    33781 gctggaactg tattaatcat cgcactaggc aatatttcct ttatttcata gaagaatttg
    33841 tgacaatcac tcccgacgaa ataaatagct tcattcagct tttgtagttg tgtgaacaaa
    33901 tcagaagcag aaatatgtac atcttcaagt acaatttcta atctattttc tttccagcgg
    33961 tatgccccag cgtatacatt ttttcttcga gcatcaaaca tgggcacaat aattttatcc
    34021 acaccgatgc aattagccgc gatggttttc agactagata ctcctactag ctctttatct
    34081 aatgtataag caatcgtttt tgctgtcgtt acacctatac gaagccctgt ataagaaccg
    34141 ggaccatcag aaaccgcgat tcggtcaatg tcttttggac ttatttgaag atctgcaaac
    34201 aattggtcga ttgcaggcat cagtgttaca ctgtggttgc gtttgatatt tgtttgtatt
    34261 tgtccaagaa tttgttcgtc ttctaccaca ccgatcgcca aagtttggtt tgcagtatct
    34321 attcctaatg taatcatata tttgatcctt tctgctatga cattctcgcc ttccattgta
    34381 gcatactttt ttattctgag gatgaaaata ccaaaggcaa aactagtaca atgatacatg
    34441 attatctgct ggcgggcaaa ttttgcccct tcctgaaaca caaaaaagct gcggtatcac
    34501 ttcatttccc cacagctttt ccatctttta tttcgttgat tctcgaataa tcaactctgt
    34561 ttccacaata atctttcttg aaaccggtgc tttggaatta cttaattgaa gaatcgtctc
    34621 cactgctagc tctcccatcc attcggtata aacttttaca gtagaaagag agggcgttac
    34681 atatttcgct acactgacat cattgaaacc catcacagaa atatcttctg gtaccactaa
    34741 acctttttct tgaatcgctt tcagtgcgcc aatcgctaat gcatcattag ctgcaaaaag
    34801 cgcatcagga acatgcggat tcgtttttaa atactgcttc actgcttgat atcctgactc
    34861 gacagaaaaa tctgcttcga taattggacc ttcgtcgtag cctttttctg ccaaaacttg
    34921 ttgaaatgcc ataattctgg gatcttttag ccgctgatgg tttttcttcg tatattctgt
    34981 tccagcaagc atagcgatat gttgcgcttt atgggagaca aaatgttcaa tgactttttg
    35041 aacacttgac ttaaagtcga caactagaga gtcgattcct tgagaagtgc cgtcagaatc
    35101 taccaaaagc aaggcatttt gggatccttt catttctaat aactgagctt cgtcgaattt
    35161 tcctagtgcg atcgttccat ctgtcggaag atcactaatg gaatcccacg tttctttcaa
    35221 taaagtaacg tttgcttctt ctgccttttt ttcgattcct agacgaatag ataaataata
    35281 aagatcttca agctcttccg tatcgtcgta ccactggatc aggcgaagcg tttgcttagt
    35341 atttgctgca tttttacgat gtttcgtata atttaagcgt tctgctgctt caaaaatttt
    35401 ttgctttgtg gctaagccaa cggaaagatc aggatcatag ttgagaactc ttgaaacagt
    35461 agcaggagaa actcctgcca actgcgcaat gtctttaatt gttgccattt gtctctcctc
    35521 cttttttacg tttataaaat tgcagtaaat cgttcaaacg ctgcctgtcc tttttcatct
    35581 cgcttgaata ctccagcgtc ttccagtaca cgtgcaaaaa cctgacctac agatttttgt
    35641 acgacatcgg tgaccgtatc tttcgtcaaa tcaggatgtt ctttttttaa ttgttctgcc
    35701 cacggacgat gatattcagc catctggttt tcttctccta ataagaaatg ctctacttct
    35761 actagttctg gtttcaaacg aggtggtaaa acagcaagcc ccattacttc gatcaaccca
    35821 atgttctcct ttttgatatg ttggacatct ttatgtggat gaaaaatccc atcaggatgt
    35881 tcttctgaaa cattgttgtc tcgaaggaca aggtccaact caaagtaatt gtcccttctt
    35941 cgtgcaattg gcgtaatcgt atggtgtggt gtaccatctg ctgaaaaagc tctgatagat
    36001 actgtttcat ccgaatatgc tttccacttt tccaatactg agttagctgc ttcaatcaac
    36061 aattttttct ctgttgattg caaacggatc acagacatcg gccatttcac gactcctgcg
    36121 atgacttgcg gataatctgc tagtttgata agtttttcta tcggtgcttt tgccatagga
    36181 aattcatgac gtcctgcctg ataatgatca tgagaaagaa tcgatcctcc aacaatcggc
    36241 aagtcagcat tcgaaccaac aaagtaatga ggcaagactt cagtaatccg aagcagtcta
    36301 gcaaatgttt cctttgtgat tttcatcggg cggtgttgtt ctgataaaat gatcgaatgt
    36361 tcattgtaat aagcataagg agaatattgg aatccccagc tttcaccgtc tagattcatt
    36421 cgaataatcc ggtgattggt gcgggctgga tagttcaagc gacctctata tccttcgttt
    36481 tccatgcaaa gcatgcaggt aggatagtct acttttaccg cctcgcgttc tgctgcgatt
    36541 tgttttggat ctttttctgg cttagataaa ttgattgtga tttccagttc accatactcc
    36601 gactctgccg gaaatttgat gttttttgcg atggctcttg ttttgatgta gtcatttttc
    36661 ttacttaaat taaagaaata ctcagttgct tcctctgggc ttttcgaata gtgctgtgca
    36721 aagaatgcat tgacgactga aggtggcggt gtgagaaaat ccattaattg cgcttcaaaa
    36781 atctcttgtt ctgctggcaa ttctgaaaga acaccatttt ctcttgcctt agcgacaagc
    36841 tgatccaata agtctacaga ttcttcctta actggtctta cttcaatgct ttctagagca
    36901 tcttctccta ccatccctaa aagacgattc tgtaaatata agcggtccat ttccatccat
    36961 ccgccagctt cgatcgcaag tgtggcaaaa tcacaaatcg tttgactgat tgacatatct
    37021 cattccccta ttcttcataa ccatttggat ggctgacatg ccaatcccat gctgttttga
    37081 taattgcttt gatatctgtg tattcaggtt tccaacccag aacctctctc gctttttcac
    37141 ttgaagcgac taatcggcta ggatctccag ctcttcttgg tgctatttct gctggaattt
    37201 cttttcctgt cacttctcta gctgcttcta gcatttcctt gactgagtat cctttgctgc
    37261 tgcctaaatt gaagaagttg ctttcatttc cttctttcaa gtattccaaa gctaaaatat
    37321 gtgcagcaat caaatcttct acttgaacat aatctctgat acatgttcca tctggtgtat
    37381 cataatcgtc tccataaaca gccaatgctt ttctttgacc aagagctact tgtaaaatga
    37441 ttgggacaag atgcgtttct ggtgtatgat cttctccgat agatgcatct gcttttgctc
    37501 cggctacatt gaagtagcgc aaagcgacat agcgcatgcc atatgcttgg tcacaccact
    37561 tcatcatttt ttccatcatc aatttacttt caccatacgg atttttagga ttagctggtg
    37621 tatcttctgt gattggagac tcttttggtt ctccgtaagt tgctgcagta gaagagaaca
    37681 cgattttatt cacattgaac tcgtgcatga cttcaagtaa gatttgcata ccgtatacgt
    37741 tgttgttgaa atacatcaat ggtttttcga cagattctcc tactaaagag cttgcagcaa
    37801 aatggattac tccttcaatc ggttcctttt caaatacgga acgtagaaat tctttatccc
    37861 ggatgtctcc ttcataaaaa tgagcatctg gatgtactgc ttgtttgtga cctgtgagca
    37921 aattatctac cacagctact tgatacccct tttgaactag ctggtctaca gcgtgcgaac
    37981 cgatataacc ggctccgcct agtactaaaa ttgtcataaa aaacactcct ttgattagta
    38041 aaattgtgtt tgttgattat atataattta aaaaaacgaa gtttactttt atggtgaaaa
    38101 caaacaaagt aaaatttcgt aaattcatat ggtaaattta taataaaaca tttagtaaac
    38161 attttcaagg attttttgca aaggtttaca aacaaacgta aaactagcct ttttttctct
    38221 tttgttccat aatagatata acaaataata aatgaggtgg attcaatgaa aaaatttgta
    38281 tctggaatat tagtaggaag tctggcaaca gccgctgccg ttgctggttt ggtcgcttct
    38341 gtgaaaaaaa cagtgatcga tccaattgac gaaaaagaag caatgatcga agaaaaccgt
    38401 aaaaaagcaa tgcgcaaacg cgtttctcgt taataattat ttaaaaacac gcatgaagac
    38461 ttgtgaaaga acagataagt catcatgcgt atttttattt gtcagtcaat ataagttgtg
    38521 ctattttatc tgcctgtctc agattccatg gatctgtata aggtctagct actgctgcag
    38581 ttagccaatc cacaccttca acaggtattc catagcaata aaagtcagac tggatttccc
    38641 ctgaagcatc aatcacgcga ttcgtacctc gctcaacatc tatagcttct gaatgataac
    38701 ttcctgtatt atctgaaaat gaaaaagggc gaacaagttt gttttccttc aaatttttta
    38761 tgacaggatt acttgaacga gaaaagcttg tttgtggcaa tcgagcttca atcaaatagc
    38821 gactgctgat ttctttatct tgtagagaaa taacatagcg gttgtgcttt tgctgaatat
    38881 caaatggcgg ttcgaacaaa tgaaaaatac ccgcatctat cagtgctgct aattctttcg
    38941 tccgaatgac tggcggaccg atcgttaaaa agctatttag atgagtaaac catccccata
    39001 gtatttcttt gtattccttt gcagaaaaaa cgtgccattc aatagcctgc cggattggat
    39061 cacgccagtc ttttaatgca tcaaatgttg aagcaaatgc tcctgtgcaa ttgccttttt
    39121 ccgcttcttt tatctgccct tcaataaatg ctcggctagc ttctgcaaag gactcatcct
    39181 gataggtaag aggaatttct aaatacgacc aatcccatct ataagggatt agtgctggat
    39241 gctttccgag acactcctct gaagagaggg agagaaaatc atgctcaaaa tttttctttg
    39301 gaatcggcaa atgatattct tcgatgattt tcttgtaata aaacaactca acgtcttttt
    39361 tcagatagtc aaaaaatgtt tcgccagtca aaaatcccga ctgatgccat tgctccaggt
    39421 tttcttttgt tagtaaccgt ggccatgcca ttgatccgcc ttgcttttgg tttcttcctc
    39481 gagggtaata agggagcccc cttcttgatc cactgtatac aatcggttcc ttgcctgatg
    39541 gatgatagac aagttttcct ttttcgttct cgaagcgccc ttctcttgat actgtaaaca
    39601 atccgacata atcaaaaaaa cttaagccta gacctcttaa aaagacattt tctttaggcg
    39661 gtatcttcga aagatcgaca tctgcaggat tagaaggaag ctgatacagt aaatcatttt
    39721 tctttgcgta gtctacccac actctttctt cttctgtctc atcattttcc cagtgaccgc
    39781 ttgctaaaac aagctgatcg atagaatagc tttgctgatc tgtctgtaaa agataaccat
    39841 cttttctttt gctgactttt tttactaaag aataaacaaa ctgaatagaa gctggaaatt
    39901 cttcttgcaa ctttgagaaa aaccagcgtt gatagagtcc gtataaacat cgagtagatt
    39961 gctgatcttt ttccagacga ttgatttctt ctataattag ttgcttattt ttggttaccg
    40021 tctgttcgat ataatctgta gcaggtcctt tactccattg aaacaagttg ggtccctgtg
    40081 ttaccaatcc tccactggac aatgtttcat ctgtaaaaag cgtcacttgt tgagaaacag
    40141 aattcattaa taactccggt ggctgattca accgccaaac tcgacctcca ggaccattgg
    40201 gatcgaaaag aaaaagttca agagaatgtc tgtcctgatt attatggaga atcctttctg
    40261 ctatacttaa tcctctaggt ccggcaccaa tgatccctat tttcatttgc atgcctccat
    40321 ttcgataaat tttttcaata aaacagcatg gttctgatgt tcattttttg caccataaac
    40381 aaaagtaatg actggatgtt ctttcataag ttgactgagt ttaaaaatag cttgcttctt
    40441 ttcttcttcg aaaatcaatt cttgctggta tttctttgca aatgaagcaa atcgttctgg
    40501 tacatggtca aaagttttgc gtgcttcatt actaggtgca atttctttca accacagatc
    40561 taactgcgct ttttcttttg aaattcctcg tggccagaac ctatccacga gtattctata
    40621 gccatcctct ttttcagcat caaggtaaac acgttttaat ttgatcatag ttgtttcctt
    40681 ctttctgcct tttcgcttag cttatatatg tagataatag cacaaaataa aaaaaatgaa
    40741 cggcacaact atccattgcg ccgttcatgt gatcttggtc aaaatctaat gattgattcg
    40801 gtttttaaag ctgtcataca atcatctgtt ttatactttt ttacttttga tttttccaga
    40861 ccatccgtca tatccaccct taagtaaata aatctcttga taacctgctt tacgtaattt
    40921 atttgctgta cggatgctca agcttttctt ttgatcgtaa atatagacag gctggtcttt
    40981 tcgaatagat cctaatgaat ctttcaaaac agtgtaaggg atgtttcttg cacctaaaat
    41041 atgcccagca tcaaacacat ctttttcgcg aacatcgatt acttgtgctt ttctcattcc
    41101 ttctcgaaat tcttcttctg tcaaagtcgt tgctgaacgc ttggccatta ctcgtagata
    41161 gacttcccaa aagatgattg caagcacaat caatagcaaa atgatattaa ttacccataa
    41221 aaccatgtaa tgcctccgtt tttttaagca aatttcaagg ctaatgaggc agcaccaatg
    41281 acaccagctt cattaccaag ctctgctaat ttgatttttg ttgattctct tacttgcggg
    41341 aaagtaaatt ctttaaaata tttctctaca cggctacgta aaaattctcc tgctgccgat
    41401 actcctccac ctaacacaat acttgacgga ttcaatgtat tccccaagtt ggcgcaagct
    41461 aaacctaagt agaagcatac gcgatcaaca accatcaatg caaacggatc gtcttcttga
    41521 gccatttcaa agatatcttt acttgttact tcttcaccgt tgtctaacat tgctttcagt
    41581 tttgaatctc ctgcgtattc ttctgccaag taacgaccta gacgtaccac accagtagcg
    41641 ctagacacag tttctaaaca tccgcgttta ccgcaagtac attcgaaccc ttcaggatcc
    41701 acagtgatat ggccgatctc tccgcctgct ccgctcacgc cgtgaatcaa atttccgcca
    41761 gcaacgatac caccgccaac acctgttcct agagtaacga atacaacgtc tggatcgttt
    41821 tctccagcac ctttccatcg ttcacctaaa gcagcaacat tcgcatcatt atcgatagcg
    41881 aatttgattc ctgttcctgc ctcgatcagt tttttcacaa attgaacttc tgtccagttt
    41941 aagttatacg caccaataac cgttccagct tcgctgtcca cgcttcctgg ggtacccatg
    42001 ccgattccga taaagtcttc ggctttcata ttatatatat tcaaacgatg gttgattgat
    42061 tcaatgattt caggaacgat atgcttacca tcatccaaaa tattggtatc gatgctccat
    42121 ttttgttgaa tctctccttc tggtgttaaa attgcaaatt ttgctgttgt tccacctaaa
    42181 tcaatcccaa taatcttctt gtccattaca aatttccttt ctttcggctt tcttcaattc
    42241 ggtgctctct ttttaatatg cgccacgctg tcatgtatgt atcatgatca atcaaacggc
    42301 tatcatacag attacgcaat tcgatagtca ttaattcaat gtcatagatg cgtgcaccca
    42361 catagataaa gatgccaaat tttttgaaca gctgctgtac atcatataag ctctccatct
    42421 ttaagcactt ccttcatatt atacaagtaa accatatttt tttaaaccca gtaaaataca
    42481 tattacaaaa agaaatacaa aaactagtgt agaaagaatc ctttggtgta tcgaatagct
    42541 tgtctgttgt ttcggtagac ccaaaacatt ccctaacact aatccgccaa ttagcccacc
    42601 gacatgtccc caaacatcta cagaggtatc cagaagacca aaaacaaatg tcaacacaat
    42661 aaataaaaga aaatgtctga ctagctgctg gatcgctggg ttatgtttaa aatgaaaccc
    42721 taagataaac aacgcaccga acaaaccgaa tatcgaagta ctcgcaccac cagacagaac
    42781 attcgcttcg ttaaaagcaa agcttgctgt atttcccatt atcccgctga acatataaat
    42841 caagaaaaaa cgccaatggc catagattgc ctcgatttgc tgccccataa aatacagtat
    42901 cagtgagttc attacgaaat gcatcagtcc aaaatggatg aagattggtg tcactaaacg
    42961 ccaccattca ttgaagtgaa caagataagg accatacatt ccggcataat aagggatctc
    43021 aagtcgcgga aataattcca tcatgaggaa gaccgctgtc tgtatcccta aaaaagcata
    43081 ggtccataac ggtcgtctta accatgcacg caattttatt tgctgttgat aattcatgaa
    43141 aacctcccat ttcattttag tcaataaaca attgttggat cttctgatca aagctctccg
    43201 gttgccattg ttcattcaat tgttcacgaa aaacaaggct acatgttttt ttatgaaaat
    43261 tatttaaata ccggtcgtaa taaccaccgc caaaaccgat tcgataaccg gctctattgt
    43321 agataatacc tggtacaatg aggagatcga tcatctccgg caaatagagc ttctctgatt
    43381 gtggctcaag aacaccaaaa ctcgaacgta caagaacgga ctgttcatcc atttcataaa
    43441 acgccatctg acgtttcggc aaagttttag gtacaactat tttcttttgt tctttttctg
    43501 ctcgttggat aaccggctgt gtatccagtt caaattcatt cgataaagtc gtccctatca
    43561 cttttgctga cttccagtac tcgctggcga atagctgttt atagattcgc tgttcttttt
    43621 cctttttctt gccacttttg gccagatttt tcagcttttc gattcctttc cggcgtaatc
    43681 ttactttcaa ttggtccaaa aactcaccct tccttctctc ataaaatggt ataggaaaaa
    43741 aagaaaaata gcaatcgaac tttcaaataa atgcataaat agaataaaaa gaagacctag
    43801 aaatgattgg ccttcctttt ttaataaatt tcaactgttc agcgcgtgtt tttgtctgat
    43861 aaaaagtgca tacagaaaat atggcgcacc aaaaagggca atcaaaattc ctgtaggtac
    43921 ttccccactt ggtagaatca ctctagcaac catatctgca ctaagcagaa aaatggaacc
    43981 gaacaaaccg ctcagaacaa aggtccagtt attttcttta cgtacgacta gcttcgcagc
    44041 atgaggagct agtagtccta caaaagataa actacctgtc accgctacac taccgcttgc
    44101 caaagcaaca gctaacaaca aaaggatttt ttgactttta gccacgttaa ggcctaatcc
    44161 ttgcgcgcgt tcttgaccaa atgatagcaa tccaatcctc tgtgcataaa gtcctcctag
    44221 cggaattaag ataaccgccc atggaagcaa tgcccaaaca taaggccagc tcgcacccca
    44281 gattgatccc gctaaccatg cattcacaaa tccataattt tccttagata attggatcgt
    44341 gcatagcaat gttaatgcag aaaggccggc attaacggca ataccggaca ataagattcg
    44401 attcataccc aaaaagccat ttcgatcata ggccgcaaga tagacacagc aagcagctaa
    44461 aacaccacct agaaaggcta caactagcaa tagaaacggc agcgaatagt gcgaaaagaa
    44521 acctaaataa agcatcacta aaaatcctgc tccggcatta atgcccaata tactagaatc
    44581 tgccaattca tttcttgtta ctccttgcaa gatataacca gaaacgccaa gacaagctcc
    44641 tgctaataaa ctgatcagta atcgaggaac tcgaaattct ctaacaatca gcgccaaaga
    44701 agatgattct ttccctatga acgtggcaaa aagctgatcc aggtaaataa aaggcgtacc
    44761 aatcattaaa ctacctatag acaggatgac aagtacaaaa agcaagaaat agaacgtcca
    44821 atatattttt ttcaccgttg ttccctcctt acttgtatca gtaaaaacgg aacgccaatc
    44881 aatgcaatga ctaatccaaa aggtgtttca aaagggggat tgatcatcct cgctgtaaaa
    44941 tccgccaaca taaccaatcc accgccaact aaaaaagaga caggtagaac ataacgatag
    45001 tccgttccaa ccattccgcg aacaactggc ggaaccaaca gcccaataaa tgaaacaggt
    45061 ccgactaacg aaacggcaat ggctgctaac actgcaacta gacagaaaac aatcaaccgc
    45121 gttcgtttag gatgacttcc aagagctaat gcatgtgttt cactcaaatg aatcaaagat
    45181 attgcttttc ctaatgaaaa agaaaccaaa acagcaagta tatataaagg aaacaatact
    45241 gctaattgtt tccatgtgat attggcagct ccgcctacaa accaaaaagc aagatcctgc
    45301 tgcagattaa agaattgggt aagtgcttgg ctgatagcag taaaaaaaga actaatagct
    45361 acgccagcta aaatcagctg taaaggattc atgcccattt tcgaaaactg caaaaaacca
    45421 aatacgagca aagttacagc aaaagaacct gccaatgaca ttagaaacac agaaaaatca
    45481 gaagctttag gccaaaaagc aaaactaagg gccattgcca acgaagcacc cgcgttgatt
    45541 cctagtaagc ctgaatctgc taaaggattt cttgtcactc cttgcatcaa tgcaccgctc
    45601 aatgcaaaac aactccctac aagaaatgct cccgccacac gaggcaaacg aatatttcgt
    45661 atgatttggt gactttgttt tgatctatca aaatcaaaca acgcctgcca aaaatctgct
    45721 tttcccacag tcgctgcgcc atttgaaaga gcaacaaaaa aaataatcaa aagaacaaaa
    45781 cagaccaaaa gcaggagtct atttttcttc atagtcctca taattgatcc tcctgctctt
    45841 ttggcaacaa atcataggtt agaaaaactg gtttatttgt tcttggaagc gtgacaatcg
    45901 ttgcatcaat ctcaaatatt tttttcatac actcttttgt gatcacttct tcaggtgtac
    45961 cttctgtgat caacttaccg tttttcattg ctaataaata atctgaaaaa cgtgaagcat
    46021 gattaatgtc atgaatcgac atcaagatcg ttgtttgttt catttgattg atttcagtca
    46081 ataactgtag gatctctaat tggtgtgccg gatctaaaaa tgttgtcggt tcatccaaga
    46141 tcaagatatc tgtttcctga gcaagcgcca tagcgatcca gacacgctgg gtttgtccac
    46201 cagacagcgt tgcgatatcc cgcatacgaa atccagctag acctgtagca tccaacgccc
    46261 actcgattgc ttcatgatct tgcgtgtcca tgccactgaa cagtttttga taagggtatc
    46321 gtccgtaaga tactacttct tctactgtca tacctgtagg gtgttcacta gtttgtgcta
    46381 ataatgtaat tttttttgca agttcttttg aactgtatgt agagatatca gagttattga
    46441 caatgatctt tcctgatttc acaggtagta tacgggtaaa acttttcaat aaagtagatt
    46501 ttccacttcc atttggccca atcaaactag tgattttccc ttcaggaatc gtaacagaca
    46561 tgttttcaat gatttttttg ttttcgtagc cggctgcaat gtcagctgct cttaatctcg
    46621 tcatttcacc catcctttcg ctgaaaatca ttctcaatta tgacgctgtt tcaacatttc
    46681 gtcaagcgct actttcatat ctatagccaa atgaagcggt tgacagcgtt cgaaatatca
    46741 ggaataataa ccaatgagaa agattctcaa ttatgttagg aggaaagaaa tgaaattaaa
    46801 aacaaaagta ttcacattat ctagcatatt gctgctggct ggagtactga gcgcttgctc
    46861 cccctcttca gataaagaaa cagctacaac tgccaattcg acgacttcat ccaatagtac
    46921 tcatacagtc acagatacac ttggacataa agtagagatt ccaaacgcgc ccaaacggat
    46981 catcggcagt tacttagaag actatctgat tgcactaggg gaaaaaccta tcgcccagtg
    47041 gactgttggc agtggttcca ttcaagatta tctgcaagat gatttaaagg atgtcccaac
    47101 gatttcttat gaccttcctt atgagaaagt gttaagcttt gagcctgatt tgctgttgat
    47161 ttcttcttcc gctactgtcg aaggcgggaa atacgaacaa tatagcaaaa tcgcgccaac
    47221 ttatgttgta aaaaacggaa cagatgtcac atgggaagaa caactaaaag atgtcggcaa
    47281 agctttggca aaagaaaaag aggcagaaaa aatcatttcc gattatcaaa aatccatcaa
    47341 gaaaactcga gaagaactag cagataaaat cgagaacaag acagctgcag tgttgtgggt
    47401 gaccaacaac tcagccttta tggtcagcaa aaatcggtca agtggaagaa tcgtatacga
    47461 cgatctggaa tttggtgttc ctgatctagt ggaagaggtc tccaaaaaag ccacttctga
    47521 ctggtctgct gtttcctctg aaaagctagc tgaattagat gctgactaca tcatccttgt
    47581 gaacagtgac aaagatgctg ccatgttcaa tgaaccaagc tggaagaatc ttaaagcagt
    47641 gacagacaat catgtactgc aattcggacc tgaatctagt tggctatata acggacctat
    47701 tgccagtcag gaaatggtca atgatatcaa agaaaaacta aattgatttt aactaaaaaa
    47761 agaatcttca cgattagtcg tgagatgctt tttttcttat taacaaacta gacgcataga
    47821 ttttattctt cttttgaagc aaccctattt tttactcttt cccatttttg aaaacgatgg
    47881 gcatatagat tttcttgatc cgttatcccc tcatactcct ctataagggc gaagtctgaa
    47941 aaatcaatat tcccaatata cgtatcgcct tcaaacgtgc tatcgatcaa tgttcgccag
    48001 atccgaccag tgtaaggaag aagttcttga aaaatccgac taccacccga aacatagata
    48061 tcttctgaga tcgtttgact atacgacaaa agttcatcga tcgaatgcat tacttccgca
    48121 ttttctttcg tgcgaaaatt tggttgcgtt gtcaacacga tgatatggcg ataaggaagt
    48181 gaaagatccc ccattccttc ataggtcttt cttcccatca ctaaaattcg atccattgta
    48241 tgttcgcgaa agaaacgcat atcatttggc aatcgccatg gtaacaaacc atttttgccg
    48301 atcagaccat ttttatcttg tgcccacatg gaaataaaca tattatccgc tccttatgat
    48361 tttagatgtt ttgagataaa aaaagtctca attctttgac cagcaaggaa ttgagaactt
    48421 taaaaacatt attttgtttc acggtgcaaa gtaactttgc gttcgcgtgg gcaatatttt
    48481 tgtttttcca aacgatcagg attgttacgt ttgtttttgc ttgttagata gttacgttct
    48541 ttacaagaag tacactctaa agtgatgttt acgcgcatgt ttttccctcc catttttaca
    48601 agaatcctct aagatgactc tcagacttga ttatcttacc atgatttttt ctagattgct
    48661 agtgtttttc acgattttgt taagtaaaac tacttgacga taaatacaac ggttttactt
    48721 ctcaaacaaa atctttttct ttttgtataa atacccttac acttgtttat atagattatg
    48781 ttttttaata aagggggata ctaatatgcc atggaacatg aaggactttc ctgcatcaat
    48841 gaaaaactta gataaattga caagaaaaaa agcaatcgac atcgcaaatg cattattaga
    48901 tgaagggtac cctgataacc gtgcgatccc aattgccatt gatcaagcaa aaaaatggta
    48961 tgaagatgct agtgaatcag aaaaacgaac tttcgaaaaa gaaaaaaatc caagcaagac
    49021 ggatgaacac gatacgaatc ctcgtgctgg aaaactgctt gattcagatg tgatcgtaga
    49081 atatgaggaa gaacaatgga tcgtgaaatc taaaggggca aaaaaggcaa gcaaccattt
    49141 tgatacaaaa aaagaagcaa tcgaaaaagg aaagcaagta gcacaaaaca aagaatcaac
    49201 tttgttcatt tataaaaaag atgggacaaa agaaaaagaa atcgattatt gaacatatgt
    49261 aaaggggttg tgacgaaaat cgtgtcacaa cccccttatg ttcgaaacaa tggtgttcct
    49321 ctgccaactc cgaaccataa gtcataatat ctataaaacg ggaagagctg ttttttgaaa
    49381 ttgtttctta tcactcagtg ttaaacggta ggagaaaacc tcttttttat tgatatttga
    49441 ttttcatcaa acagtcacat agtcgattgc ttctgcttgc ggaatcgttt catctacaaa
    49501 gtagacttta taccatgtca agaaactgaa gattgtccag atttttcttt gctcttcttt
    49561 acgtccttcg tgatggtcat ctagcaactt catgattttt tcttgatcaa aaaattcttt
    49621 tgcaaaatct gcttcgaata atgctttgac ttgttcgtag ccgtgttctt ctttcaacca
    49681 agctttgatt ggcacaggga atcctaattt cacacgattc gaccattctt ccggaagatg
    49741 tttgttcgca gcttgtctga aaatatcttt tgtgttgttt tgattcaata aaaacttact
    49801 tggtatttgt tgagccaatt tcatgacttc gatatccaat aaaggtactc ggacttccaa
    49861 agaacttgcc atagaaagtt tatctgcctt taacaagata tcttttggca tccattgatg
    49921 aagatcgaca tattgcatct tattgacttc atctttgatt ccttctgttt tctcgtaatg
    49981 aacagtcatg atttcatcca cagatggtgc tgtttgatat tttggtgtta acacttctga
    50041 tgcttctgat tcttcgaaca ctcgagcatg accgataaaa tattcttttg caggagcaag
    50101 tgattcatat aaatggatcc gtccatggaa atttttcatt tttccgattt tacgtcctaa
    50161 attatagcgt gttccttttg gtaatttttt caatccttga gcaatcacac ggatgaattt
    50221 tgaattcgta tggaatccat acgtttggta tcccgcaaac agttcatccg ccccttctcc
    50281 agattggact acccgcacac tttgtgaagc aagatttgct aaaaagtaca aaggtacgca
    50341 agaaggattt gaatcaggtt catctaagtg atactggatc aaagggaatg ctttgaacga
    50401 catgtctccg tcgatcactg ctgctgtatt atctaagtcc aatagttcgg ttagttttct
    50461 ggcttcaatc gcttcattat aggttttatc atcaaacccg attgagaaag aatgatctgg
    50521 tttcaataca gaggttacat aactggaatc aaccccgctg gataagaaag aaccaacttc
    50581 tacatcactg accgtatgtg cttcaataga agcttgaacg gtttcgtcga ttttttcgat
    50641 ccattcttgt cgggaatgcg tttcttttgt ttcaaaatct gcatcccaat attgctgaat
    50701 gtctagtttt ccatctttat acgtataata atggccttca ggcaaacgat agacatcttt
    50761 gaagaatgtt tcgccgttta atggagaata ttggaaagtc atataaggtt tcaacgcttc
    50821 tttgttcaat tctttgttaa aatcaggatg aggcagaaaa cttttgattt ctgaaccaaa
    50881 cataaatgtt ccgttcattt ctgcataata atatggtttg atcccaaagt ggtctcttgc
    50941 accgaataac tcattctttt cattatccca aatagcaaaa gcaaacattc cgcggatctt
    51001 ttgcaataat tctgttcccc attcttcgta accatgcaaa aggacttctg tatctgcatg
    51061 tgtttgaaaa acgtggccag ctgcaatcaa ttcttctctc aaaggctggt aattatagat
    51121 ttcgccatta aaaataatga tctttgtccg atcttcattg taaataggct gagtaccgcc
    51181 ctctaagtca ataatactta atcttctgaa tcctaaagca acatgtttat cgatatattc
    51241 tcctgaacta ttaggtccac ggtgaacgat tcgatccatc attgtattaa taattgtttt
    51301 tttgttgtcc ttgtcgttaa caaaacctac aataccgcac atttaacttt cacatcctaa
    51361 tataaaatta ctgtttattt cattcatatg catcttatca tagaataaat agcggacgaa
    51421 tattttctct cttacaattt cgtcacgtga aaacaaaaga tgaaagtttc tgtttttaca
    51481 gaaaataaag tcccaatatc taatttacca tgttttcttt tttgaggcta ccttattctc
    51541 ttcgtgttaa aaaaatatta gtggatgcta tttccaaaaa aaacagcaaa aaaagacacg
    51601 acctaaagat cgtgtctaa
//
LOCUS       DACDIH010000012        51613 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDIH010000012 DACDIH010000000
VERSION     DACDIH010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.012"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..821)
                     /locus_tag="IV281_000729"
     CDS             complement(<1..821)
                     /locus_tag="IV281_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8779765.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKA"
     gene            complement(992..2356)
                     /locus_tag="IV281_000730"
     CDS             complement(992..2356)
                     /locus_tag="IV281_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8779766.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLTRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(2353..3912)
                     /locus_tag="IV281_000731"
     CDS             complement(2353..3912)
                     /locus_tag="IV281_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8779767.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(3968..6448)
                     /locus_tag="IV281_000732"
     CDS             complement(3968..6448)
                     /locus_tag="IV281_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779768.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(6432..6767)
                     /locus_tag="IV281_000733"
     CDS             complement(6432..6767)
                     /locus_tag="IV281_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779769.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(6781..7815)
                     /locus_tag="IV281_000734"
     CDS             complement(6781..7815)
                     /locus_tag="IV281_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8779770.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(7905..8585)
                     /locus_tag="IV281_000735"
     CDS             complement(7905..8585)
                     /locus_tag="IV281_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8779771.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(8869..9972)
                     /locus_tag="IV281_000736"
     CDS             complement(8869..9972)
                     /locus_tag="IV281_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8779772.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTTPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(10601..11071)
                     /locus_tag="IV281_000737"
     CDS             complement(10601..11071)
                     /locus_tag="IV281_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP8779773.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            11401..13041
                     /locus_tag="IV281_000738"
     CDS             11401..13041
                     /locus_tag="IV281_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP8779774.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            13195..14517
                     /locus_tag="IV281_000739"
     CDS             13195..14517
                     /locus_tag="IV281_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP8779775.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(14585..15019)
                     /locus_tag="IV281_000740"
     CDS             complement(14585..15019)
                     /locus_tag="IV281_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8779776.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(15084..15920)
                     /locus_tag="IV281_000741"
     CDS             complement(15084..15920)
                     /locus_tag="IV281_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP8779777.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(16662..17288)
                     /locus_tag="IV281_000742"
     CDS             complement(16662..17288)
                     /locus_tag="IV281_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779778.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(17583..17918)
                     /locus_tag="IV281_000743"
     CDS             complement(17583..17918)
                     /locus_tag="IV281_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8779779.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(17905..18189)
                     /locus_tag="IV281_000744"
     CDS             complement(17905..18189)
                     /locus_tag="IV281_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP8779780.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(18245..19768)
                     /locus_tag="IV281_000745"
     CDS             complement(18245..19768)
                     /locus_tag="IV281_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8779781.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(19761..20936)
                     /locus_tag="IV281_000746"
     CDS             complement(19761..20936)
                     /locus_tag="IV281_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8779782.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(20940..21125)
                     /locus_tag="IV281_000747"
     CDS             complement(20940..21125)
                     /locus_tag="IV281_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779783.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(21091..22785)
                     /locus_tag="IV281_000748"
     CDS             complement(21091..22785)
                     /locus_tag="IV281_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8779784.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(22782..23255)
                     /locus_tag="IV281_000749"
     CDS             complement(22782..23255)
                     /locus_tag="IV281_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP8779785.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(23324..23479)
                     /locus_tag="IV281_000750"
     CDS             complement(23324..23479)
                     /locus_tag="IV281_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAP8779786.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(23613..23993)
                     /locus_tag="IV281_000751"
     CDS             complement(23613..23993)
                     /locus_tag="IV281_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8779787.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(23997..24212)
                     /locus_tag="IV281_000752"
     CDS             complement(23997..24212)
                     /locus_tag="IV281_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779788.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(24215..24622)
                     /locus_tag="IV281_000753"
     CDS             complement(24215..24622)
                     /locus_tag="IV281_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAP8779789.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(24888..26363)
                     /locus_tag="IV281_000754"
     CDS             complement(24888..26363)
                     /locus_tag="IV281_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAP8779790.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(26353..27201)
                     /locus_tag="IV281_000755"
     CDS             complement(26353..27201)
                     /locus_tag="IV281_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP8779791.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(27238..27600)
                     /locus_tag="IV281_000756"
     CDS             complement(27238..27600)
                     /locus_tag="IV281_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779792.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(27900..28524)
                     /locus_tag="IV281_000757"
                     /pseudo
     CDS             complement(27900..28524)
                     /locus_tag="IV281_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(28521..28832)
                     /locus_tag="IV281_000758"
     CDS             complement(28521..28832)
                     /locus_tag="IV281_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779793.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(28876..29169)
                     /locus_tag="IV281_000759"
     CDS             complement(28876..29169)
                     /locus_tag="IV281_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779794.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            29363..30010
                     /locus_tag="IV281_000760"
     CDS             29363..30010
                     /locus_tag="IV281_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8779795.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            30071..31216
                     /locus_tag="IV281_000761"
     CDS             30071..31216
                     /locus_tag="IV281_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8779796.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(31297..31602)
                     /locus_tag="IV281_000762"
     CDS             complement(31297..31602)
                     /locus_tag="IV281_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP8779797.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(31627..32058)
                     /gene="ruvX"
                     /locus_tag="IV281_000763"
     CDS             complement(31627..32058)
                     /gene="ruvX"
                     /locus_tag="IV281_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP8779798.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(32055..32321)
                     /locus_tag="IV281_000764"
     CDS             complement(32055..32321)
                     /locus_tag="IV281_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP8779799.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(32576..33301)
                     /locus_tag="IV281_000765"
     CDS             complement(32576..33301)
                     /locus_tag="IV281_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP8779800.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(33662..33732)
                     /locus_tag="IV281_000766"
     tRNA            complement(33662..33732)
                     /locus_tag="IV281_000766"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33698..33700),aa:Gly,seq:ccc)
     gene            33849..34601
                     /locus_tag="IV281_000767"
     CDS             33849..34601
                     /locus_tag="IV281_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP8779801.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            34617..35096
                     /locus_tag="IV281_000768"
     CDS             34617..35096
                     /locus_tag="IV281_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP8779802.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(35347..36927)
                     /locus_tag="IV281_000769"
     CDS             complement(35347..36927)
                     /locus_tag="IV281_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP8779803.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(36920..37663)
                     /locus_tag="IV281_000770"
     CDS             complement(36920..37663)
                     /locus_tag="IV281_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8779804.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(37839..38615)
                     /locus_tag="IV281_000771"
     CDS             complement(37839..38615)
                     /locus_tag="IV281_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8779805.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(38863..39699)
                     /locus_tag="IV281_000772"
     CDS             complement(38863..39699)
                     /locus_tag="IV281_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP8779806.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(39710..40639)
                     /locus_tag="IV281_000773"
     CDS             complement(39710..40639)
                     /locus_tag="IV281_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP8779807.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            40831..41184
                     /locus_tag="IV281_000774"
     CDS             40831..41184
                     /locus_tag="IV281_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8779808.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(41258..41914)
                     /locus_tag="IV281_000775"
     CDS             complement(41258..41914)
                     /locus_tag="IV281_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8779809.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            42140..44074
                     /locus_tag="IV281_000776"
     CDS             42140..44074
                     /locus_tag="IV281_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8779810.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(44160..45383)
                     /locus_tag="IV281_000777"
     CDS             complement(44160..45383)
                     /locus_tag="IV281_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP8779811.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(45377..47275)
                     /locus_tag="IV281_000778"
     CDS             complement(45377..47275)
                     /locus_tag="IV281_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP8779812.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(47269..48387)
                     /locus_tag="IV281_000779"
     CDS             complement(47269..48387)
                     /locus_tag="IV281_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8779813.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(48470..49303)
                     /locus_tag="IV281_000780"
     CDS             complement(48470..49303)
                     /locus_tag="IV281_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8779814.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(49296..50213)
                     /locus_tag="IV281_000781"
     CDS             complement(49296..50213)
                     /locus_tag="IV281_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8779815.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(50440..51564)
                     /locus_tag="IV281_000782"
     CDS             complement(50440..51564)
                     /locus_tag="IV281_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8779816.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT        15364 a        10433 c         8677 g        17139 t
ORIGIN      
        1 tgtgctttca ttacaccaaa gatattaatc ttttgcatcg tcagtacata aatgaaaatc
       61 ccgatcacaa tagccgcaat aacgatcaaa aatccgatca taaatccaaa agtcaatact
      121 tgcgcattat atccaggtaa ttcattgatg aatttagaaa ttttgatctg ttctaaatct
      181 tcaggtaaat tactgatttt acctctgaca actattgcgt taatccgtgc attttcactt
      241 gtatcttctt tttcgaagcg gatctcttgg taagcattga tggttgtata gagaactggt
      301 gagacattga atttggcatg atccgtaaag ccagttagtt taaacgtctt gtcactacca
      361 gataacttaa ctgtatctcc tacggctaga ccatattctt ctttcaaact aatatcgcct
      421 actgcctcat catcgttatc aaaagctttt ccttcaacaa gattgggcat gatgaattga
      481 tttttatcga taccgaagaa gctaacattg atttttcctt cttctgtact gtctttacta
      541 gtcacgactc caggtgtctg ggcaagatac gccactttcc caccttcgac ttcttctgct
      601 atcttcttag taatcatgga catccctaga tttgaatttg catctttcga aagcacaatc
      661 gaatctgcct cccacttgtc gactgctgtt cgattatctt gagctaagcc ataagctaac
      721 ccagtcaaaa aaaataccag gtaggcgatc aaaaacatca cccctgcaac taacgcataa
      781 cgtaactttg aatgcataat ttcatttaat gctaaaaaca tttaactcct cctcctgaaa
      841 taatcatttt atacaacaat tttatcactt gataaatttt tggcgaatat tatgctcaaa
      901 aaattcttcc gctatttcat tatatgaatt ttttattctc gattgattaa tatttattat
      961 atccccacac aataaaagac cgtgatattt ctcacagtct tttatctact ctattgctat
     1021 ttgctctctt agctagtact ttaaaagcta actcattgag gtagtgaaaa cgagaggtgt
     1081 gttcagacag aaatccaaaa tcctcaactg ctagctgtgg aatcgtagtt ttgtgtttca
     1141 cgatatccgc aagcaggttc aaatctgata ataagaattc ttggcttatc agctgtccgc
     1201 caattatttc taatgtatcc ggatggtaga tcagctttgc atttactgtt ttgttttctt
     1261 catcgtattg tggaaataga tcaaattctc ctgaacacga accggtattt tgttccaaga
     1321 atggcgcttc atcttctgtc agccctacag ttcctaaaaa gtagtctata atgactgctg
     1381 ctgttatctt ttgtctgagg tcgtaagcgg ttggctgacc actcaaagtt agcgctactg
     1441 ctcttgccat atgtacagct cgagtaacta aaggtaaaaa gagatgccgt ttatttttgt
     1501 caaaactgac tgggacgata tctccaatcg catagatgtc cggatcactt gtctgcagat
     1561 attcattgac tatcacagtc tcatcctcat acaaagccac tttttctttt aacaatgtcg
     1621 tattaggacg agtttgggca gaaaaaatga ccatatctgc ctttattgct tgagatagaa
     1681 gttgtacgga tactactttc tcttctgatt ttttccaatc gatcaagaat tcatttggat
     1741 aaaaattgat ttgcttagtt tgcagctgat tttgaaaact gttgatcata tccttatcga
     1801 aatatcttgt taaaacattc ggcatataat caactagatg aataatgtat cctttatttc
     1861 tcaaagcatc tgctaattcc aatccaatat aaccagcacc gaagatcact acttcttttg
     1921 cttcttttaa agcggcaagt acttgcttag attgcactag atttttatag gtgtagacat
     1981 tttcagcttc aatttgttta tacaaggtga caggagagga acctgttgct acgattaatt
     2041 tatcataggt tatggtctcg ttttttcctt ctttggatgc gaggatgagc tgatgtttgt
     2101 ctggatcaag ttcagttact ctccattcgg tgatcagttt tgccccactt tcttgaagag
     2161 tccgcatgtt atgggcaact tcagctaaac tacgtatctt tccttttaaa taaaggttga
     2221 ttccatttga aacatagcct agtccatctt tatgatcttc atcgatcagt aaaacttctg
     2281 ctttcggttg aagttttctt agatttaggg cagctgtgat gcctgcatgg gaagcaccaa
     2341 tgatcacgat tttcatgttt tattcatcct taaattcaat ttttctaaat aacattcttt
     2401 taaaaattgg ttcaaatagg ggaaatcata attatattcg ttgctagtga atacaaaaac
     2461 tttagcggac ttctgtgacg ggtactcttt caattggcta gtcaaaatga tatcatactg
     2521 cttttctttc tcgtattcga ctaaatcata tttgatcgta ttcagcaatt gttcctggat
     2581 gatttctttc acgatatccg ccattagcct ttcataagga agatcataag caatttgcag
     2641 acgtaattct aactgcttga tcagcgtccg caaaatagat gcatagcgag caaataattc
     2701 aaagcgggca ttttcctcgt ctacttcagt aaaatcaaca atagcaaatg tttgtctgac
     2761 caatctttca cataaaacca ggaattgttt atcttctact tcagttaacc gctgttttaa
     2821 tccttcttgg ccaaaaatat tcatccatcc tctaaaatag gcaaaatgag cgtgattttg
     2881 catcactgtc aacgcaatct tttgcgaaat ctcttctgga aaaacataaa aagagaaata
     2941 ttcttctatc gattgtttaa acgttcgatt caattgagct actacgtcgt ctttgtattt
     3001 ttcgcttatc aaaaactcct ggacatgcga gttatgtgta tctaatgtaa agttactgat
     3061 catgaaaaga taaaaaacca acgtttcatt ttgattccaa ggaaaggcat attgaaagaa
     3121 gtaacgggtc aaaaatgtat ggaggtccaa taaaaattgt gtcgtttgaa acaatccaac
     3181 aggaagattg ctataatctg gtttggattt tttcgtacag cgcattcgct tcttcataat
     3241 ggaaagccat atttcaaatt tcaatcgtcc ttcttctgta atagtaaaac taaactcttt
     3301 ttgcagcaga tctaaaaaag tgtttcctga taaatcactg tattcttttt tgttttcaag
     3361 ataaggacgt ccaaaccaaa ataattggaa ataaaaataa caaatttgac tttctggacc
     3421 ggtcagccgc ccgcttttta ttttcagatg aaactcattt aaagaatcat ttaattcctt
     3481 cactctgcga gacaaagtag ccgaactgat attaagttcc atgacaagtt tctgccgatc
     3541 aaatgagcca ttttccaaca gataaagaag tatctgatat tttgtagaat agcgcaagta
     3601 gcaatgataa taaacatcga ctgctgctat atttccatgt ctgattaata aaatattccc
     3661 ttcttggata acaatttcca attgtttgat attttccaag ttagcttgta aataaaacag
     3721 atcttctttc aaggtagccc gatcaactga taagcgggta agtacgttat taacggaaat
     3781 ctctcctttt tcctctaata aaagttggaa aagtttgatt tgccgttgtt catttttttc
     3841 aagcaaactg aaaagttcca agtccactcc tccaagcatt tctgatttta acagcagaag
     3901 tttccttctc atccttgaaa aagaggatct gtgacagtta tcacagatcc atacaaattc
     3961 taatcactta gctggttggc ccagctgtca gttcccatgt gaccatgcct tgatacgttg
     4021 cgccaggttg agcattcgta ctgctattta cctctaagag taaaccatcg gtatcgggaa
     4081 agtcaacgag aacctctcct ttagctgttc catcactctc ataaatcaac gtgttttgac
     4141 cttgctgaat caattgtttt gatccagaat gattgaagta taattttgtt ccgcttaatt
     4201 cttgaccttg atcattctta aataaagaag tcaatgtccc tgtcagttgc caaggagacg
     4261 tgcttggaag ccgagtatcc cgcacatcaa aagaaaaatc gccttttgct tcataataac
     4321 ttgtcttcaa cggtttgggg agatcgttac caaatgtaaa atcatctggt acattaacta
     4381 atgttaattc ttgtttgggg acaagaattg ttttagcaga tgtttggaca actgggttag
     4441 gaagcttagc accatctgct aatgtaatat tgctggaaag tacagctgcc tttgtcagta
     4501 tgctgacgga ttgtacagaa gtatctacag tatttaattg atagttgtaa gtaagatgaa
     4561 tggtatctcc tattttgctc aatagatctg ctgggacaga aactccttgt tgaatgccgg
     4621 tatctgcgat agttgtacca tttaatttta aggaaccatt gatataagta gtattttcag
     4681 gaagtgagat cgtgattttt ttgtcggtta tcgcatcccc agattttgtt aacgtgaatg
     4741 tcacattttc ttgaatcgta tctcctgata gagcagacag gggatctcct gtctcaggtt
     4801 gtctgctaag gttttttagc tgactatctg cactcacaga tggaaaatta gccactgtta
     4861 tttgaggaag agtggtgatt ccagcttgtt cagactgcca tttgtacgca aaagacggat
     4921 acatgttagt aagtacatcg tccaccaagc gataatcaaa ataatattgc cctgaagcca
     4981 atgcaggtag tgtagaagta gctgtattta cacccccgtt atttgattgg atcgtttgga
     5041 aaagttgatc tgtccatcct gtcgctaatt tttgggttcc tttcgaataa agcctgtaat
     5101 ggatcgaacc cttttttagt tcagttgctg accaattggc cgtgacttgg tacgatgcag
     5161 aagaaaggag ctttccccac tgactatcgg ctgttaagtt tgttggtaaa accgttccgt
     5221 ctagcttgta ctgctccaat acctttaaag caacaggaga aagcaatccg ccttgcgcat
     5281 ctttcccact gtacacgata ttggctgtct gattcaatcc acttgtgctt gctggagtat
     5341 gagtcagtat tgctccttgt gtaacgtttt ttcctgtaaa atagttgact acgcgatcaa
     5401 agcgactaaa tggcaactga tcaaaacgtc cttcagtcag ccccatcgtc aatgtccgac
     5461 tattggtaga gcctggaagg ttctggttat ttccatccac gtattctgtg gtatcagcaa
     5521 atgtattata ttttcctgtt gaatcacttg ttgagatgta actattccac gtatttatct
     5581 ctgcacttgc agaaaatgca tatgctcctg gattcaagtt tccgccagtt aatttaggat
     5641 aaaatctacc tgcagctgga gctaaaatcg aattgaacat acgatcttgg ataatattat
     5701 tcgaatcata aaaatcaaca gaaaaaacgg cctttttaga catagaaggt tgtaatttac
     5761 ccttggtcaa aattttgttt ggactcgtat catatttatc tccgttttct gtcatcttcg
     5821 cgacattagc tgtaacatca aattgaagcg ggatatcgtc cacgttgaat tcgcctcgcg
     5881 taataatagc aataatatct gccaatagag tatttgtatc ccacgacccc atcccaaaac
     5941 gcaaggtatg gcttcctgct gataataaac taccgctagt aaccttgatg ggggcaccgg
     6001 caatatacaa gtaggcgttg ttccagtcaa ttgcttttaa tacatcactg cttgatacat
     6061 gttccggcaa attgatgatc gccacaaatg caaatccttg tgcagtgata ttcaccaaat
     6121 caagtacatt tgttagtttc acatggcttt cccaatgaaa acggaccgtg ccacctttcc
     6181 aaggatcatt tgaaccatcg ttcaaataag tagcagtcaa tttaggcaat tggttagccc
     6241 cagttagttg gacaagcttc ccgccattga ctgcactacg tccaccattt ggaggtacga
     6301 tattttcata cgtgtatgga tcataagaaa tggcaggagg cgttgctcca acagcttctg
     6361 ccgaaggtga aaagataaat atacttaaaa caaaaagcga agtaaaaaag aaggagcgaa
     6421 aaaatgaaaa tttattttgt agcaacatgt tcatcctcct tttcctttct tctccataac
     6481 aacaaccacg caagaagcca taattctata ccaatgatca aataaagaaa acggatcttt
     6541 tcttcattcg tctccgggaa tacgccatgt tgattagatt ctttataaat cggtagctgt
     6601 gtctgctgtt ttgatgcgcc attgactgaa ccacttggat gcggaactgg acttagatca
     6661 taaaatgtaa tcgtacctga acttttggta tctgcttgga ctggctcttg ccagctgaag
     6721 aacagcaaac ttgcaataat ccctcccaat aagatcgatg ttttcaacga aatcacctca
     6781 tcatgttgtt tctccttttt tctttttctt gtggacccac aaaaacagca aaatcaacca
     6841 taacactaga actgcagcaa ccaatcctgc ataaagccat gggaaagatt gttcttgtac
     6901 tgcaaaaacg gaattttcat ttactttttg tgctttttct tttttgatcg taaagttttg
     6961 tgtaaatcgc cattcattac tgtcgcctga ttttgctatg atttctgcgc ggtaatcgcc
     7021 ggcttttgct ttatcaccag ctaaatcaag attcaagtga aaattcgaat ttggtgcaac
     7081 taaaaattca ttagacgtat tttcaatata aagctgattt tcataaaaga tcttgctcgt
     7141 aaatatcaaa ttgctgataa tcctaggctg aggattttgg aaacgaagct gaacagttgg
     7201 ctttgcgttg acctcgtcca aatctcctga taaaagtttc agatccggtt ctatcgtctc
     7261 ttgactttct tgtaaaacga ctgcgagatt ataagcaata cgatttttga tttgtgctcc
     7321 ttctgtagca ttttctgttt cagcttcttc tttaggtgaa acatgaatac ccgctaatac
     7381 acgtccctta aagctttttg ctgggatttt cacttctata ggtatagttg tttctgcatg
     7441 tgctgataca ttgatttcct ttttcgcaat gagcaaaacg tcacggacat caaacggcaa
     7501 tgtctcatct aacgttgggt ttttttgact ataatcgatc gttccaccat cactcgttac
     7561 cgccggattg acagagacct caaatgttcg ttcttcatct gaagaattgc tgataattac
     7621 ttctagtgtc tgattttgtt ccggtgccac taataaatca aaataaccga ttgctttatc
     7681 tatttggttc tctggaaata ccggtttgat tccaaagtct cctgctgaat cctttgcttg
     7741 gctgattgac ggaaaaatga aaaatagact taaaatagaa aaaaacgaaa caaacagtcg
     7801 tttactttga ttcattgtcc accacatcct ttcaacaaca actgcggtaa aaaatccttc
     7861 acctatagta ttttcaccgc agtttgttga atttatttca tactctatgc tactgggcca
     7921 gccacgaggt tccaagtcaa atcagccgtg aatgttccag ctgctgcttt cggcactttc
     7981 agtgtgatat ttgctggatc ataaacagaa agccatgttc ccagaccttg atcttttgct
     8041 gctgtaaata ttggtgtcgc tgcacttgct ttactcaacc cagttacagt ttgactagtt
     8101 ggtccagtgg attcgttatc tgacgtactt ttaacagtgg atgttccaaa atctaattca
     8161 gcgccttgca aaacttgtga accgtttaca aaatctgaaa ccgaaactgt cacgttccac
     8221 ccttgtgctt ggccatctgc acctacacct cgacgatctg atacttggag ataaggtgta
     8281 tcgttttttg aagcgtctac ttgatacgtt tttgttccgc tttcaatctc gtgtgtacca
     8341 aatggcaatt caggaactac atccaatgtt aaagaacctg tctggccagt tgctggattt
     8401 tccggatctg ctggctctac tggttttgaa ggatcagtag ggttaagagg ctcaacggta
     8461 tttgttggtt cagtaaaggt aatatctcct tttgaagtcg cttgaccctc agcaaaagct
     8521 ggtgttgcca agccgcctag aagaagtgtt gaaagtaata atgtcccaat catgttcttt
     8581 ttcataataa atcactcctt tttcttagtg acctaagtat agtagagaca gtatgcaaaa
     8641 aatcctcatg aattcatccg cttacttgaa attatgaccc tttttgtata aaagagaaaa
     8701 aaacaataaa atcgtttaca tgaatattct tcctttttta ttaagtatga aaaaatattt
     8761 caattttatt ttttcgtttc aaattattct attagtgaca tccttgaact ataaaaatag
     8821 gagtacggca tcatctgccg cactcctatt tttttcgtca tttatttctt aaataaaacg
     8881 attctcacgc aattttattt ttttgatatc ttctactgtt tgtgttccag ctaattgcat
     8941 gaccatttcc aattctgttt taaagaaatc aaacacttga tggactcctg tacttcctcc
     9001 taaagacaat ccataaataa cgggtctgcc aatagcaaca agatcggcac ctgatgcgat
     9061 tgctttgaaa acatgttgtc cacgtcgtac accgctgtca aacacgattg gtacacgttt
     9121 atctactgct tctgccacat attgcaacga atcaaacgca gcaggccccc catctaattg
     9181 acggccacca tggttagata cccaaattcc accagcacca gattctaatg atcgatagac
     9241 atcttcttct gattgaacac ctttcacgta gactggcaaa tcagaatgag cagcgataaa
     9301 ttctacatct tttggactaa gtttttgttt ggaagattta tatacagcat ccatcgtttg
     9361 gcctactcct gattgataag cctgaacgat cggcattggc agcgggaagg taaacccgtt
     9421 tctgcggtct gtttcacggt ttccgccaac tgttgcatcc gctgtcaata cgattgcttt
     9481 tgctccatta cgcttggcca cttcaagaat atccaaattg attccattat ctttgctcat
     9541 ataaaattga aaccattgcg gtgctttctc cccgcctgct tctcttatct cttctaaagt
     9601 acaagaagcg taagaacttg ctgtatagat cgtcccaaaa tctgctacac cttttgcaga
     9661 agctttctcc gccttcacat gagctaatcc atgagcagca actggtgcca tgatgattgg
     9721 tgtagtcagc atttcttcgt caaaatgtgt agttgtatcg ggtaattcta catctcttaa
     9781 tacatgagga ataatcaaac gatgattgaa tgcccgttca ttttcttgat aagtaaataa
     9841 gtcgccagca ccactgctga tgtaaccata accaccagct ggaatcactt gtgaagccgc
     9901 gatttctaaa tcttccatat tgataaaatc tactattcct tcatttgtac ctgcttgata
     9961 tgttttttcc atctcaatgc cttctctcta taaaatttga atgatgaaca atatgatagc
    10021 gtttacactt tttatattac tcttcgacat tgatacaggc aaataatctg tttacatttc
    10081 agccattcca gatgtcacta ctctggaaga aatgattgta ataaaaatag ataaaaaacc
    10141 gatgggactt tgacaaaagt agtgtcaaag tccctttttt tgaataaacg gtgttcaaaa
    10201 gccattttct cggtattaag tcaaatttat gcaaaaatgt ggaaagccat tttcacaaaa
    10261 tttttcttac tactcgaaac taagcgactt tttcacaacc tctcgataaa acggtgttca
    10321 atcagctgac cctcggtatt agctcaaaaa atggcaaaat atgaggaact attttgccat
    10381 ttgtctctct aatactcagg tcagaacgtt gacttcacag tctcttcctt aaacggtgtt
    10441 caaaaacttg catctgcggt aataagccca accaaacgaa aaataggagg agctattttt
    10501 gtttggctgt tcttattact tgatgcaggg caggtttttc acgacctctg attcacggtg
    10561 tttctttttc atcaacattt ttacgaaata ctgtggaaga ctattgaaat aagtgtggat
    10621 tgtttctttg tgtttcaatc gtttcttgcc attcttcatt taattttgga ttaccgtttt
    10681 cttccttgaa ctcataaggt cctaattcaa aaatcccaga cagtatttga ttcacgattt
    10741 catatagttc agtgtgatca gacacatgat tcttgaagat catctcatta tcaccacgtt
    10801 tgaacctcac attcgggctc tcatgatgaa cctctatgca tttttctcct ttttttaact
    10861 gacggacttc tgaaaacatc tcataaatat cttctccagt aaaaccttca gcaagcgcca
    10921 gactgtccac aagaccgcct actgtcagtt cagagattat tgcttgattg gaatctaatt
    10981 ttaatgtata gcttgtcgct tctactacgc cacatgcatt gcaaccgcct tctgatagtt
    11041 cgctttttct aatcaattga acttctttca ttttctcttc ctcctcttaa ttttctactt
    11101 atgtattttt atcacctgac ataggtgact cctatcaacg ctatcatgtg cttttttcct
    11161 cagaaatcct atttatccat tccatctatc ccttattctt ctacttttct ttctctagca
    11221 tataagagat acgcatagta ttcaagcaag gattgtcgat ataaaaagaa caaatactgc
    11281 tattttgatt tcttttggct atattacata taaataaaat ttatcatacc tgctcttttt
    11341 gactatatca tcatctgact tctatacttc gtttgtatta aaacttagga ggaatcattc
    11401 atgcttacag ggttaaactt attaaataat ccttttctaa ataaaggaac agcttttaca
    11461 aaagaagaac gggcaaaata tggaatcact ggtatgcttc caagtactgt ccaaacactg
    11521 gaacaacaat ctgttcaagc ttatggtcaa tacttaagca aacaaacaga tttggaaaaa
    11581 cgtatcttct taatgaatct attcaatacg aatcggacat tgttttataa attgatgggc
    11641 gaacatttag ttgaattcat gcctgtcgtt tatgatcctg tcgttgcgga ttcaatcgaa
    11701 caatacaacg aaattttctt gaaaccgcaa gatgctgcat ttctttcaat cgacgaacca
    11761 gaaaacatca aagcaagttt gaagaacgcg gcagatggac gagacattcg tttgattgtt
    11821 gtgactgatg cagaaggaat cttaggtatg ggcgactggg gtgtgaacgg tgttgacatc
    11881 gctatcggca aattgatggt ctatactgct gctgctggta ttaatccagc acaagttttg
    11941 cctgtatcga tcgacgctgg tactaataat gaagaattat tgaataatcc cttatactta
    12001 ggcaatcgtc atgctcgtgt agaaggcgaa acttactatg aattcatcga tcaattcgtt
    12061 caatcagcta ccgaattatt cccagaattg ttattgcatt gggaagattt tggacgagga
    12121 aatgctgcag cgatcttaga aaaatacgaa gatcaaatca ctactttcaa cgacgatatc
    12181 caaggtacag gaattgttgt cctagcaggt gtattaggcg gattgaatat atccggtgaa
    12241 caattgaaag atcaaataat tttgacattt ggtgctggta ctgctggtgt gggtatcgca
    12301 aacatccttt tagatgagat gatccgtcaa ggtgtacctg aagaagaagc acgccaacat
    12361 ttctatcaag tagataaaca aggtttattg tttgaagata cagaaggatt gactcctggt
    12421 caaatcccat tcgctcgcaa acgctcagat ttcactaatg ctgatgaatt gacaaactta
    12481 gaagcagttg taaaagcgat ccatccgaca atcatgattg gtacttctac ccaacctgga
    12541 gcattcactg aagaaatcgt gaaagaaatg gctgctcata caccacgtcc gatcatcatg
    12601 cctttgtcta atccaacaaa attggcagaa gcaaaagcaa aagatttaat agaatggacg
    12661 gacggaaaag cattagttgg aacaggtatc cctgctgctg atgttgaata taatggtgta
    12721 acttaccaaa tcggtcaagc aaataatgct ttgatgtacc caggtttagg acttggtttg
    12781 attgcttcaa cagctacacg agtaaatgct gaaatcattt ctcaagctag tcgagcatta
    12841 ggcggaatcg ttgatgtaac aaaaccagga gcagctatct tgcctccagt agcaaaaatc
    12901 actgaatttt ctcaaacgat tgctgaaact gtcgcaaaat cagttgtcgc acaaaacttg
    12961 aatcgtgaag aaatcacgga tatcaaagaa gcagttgaat ctgctaaatg ggttcctgaa
    13021 tataaatctt tagaagatta agatcgttat agtaaaagaa catacagaaa ctttgtatga
    13081 cgtctaaaaa gaaaaaagcg actgcggcaa aacagcggcc agccaactcc tgctcttttg
    13141 tcgcttttct ttggcttttt tcatatgata cagaataaga aaggatctgt aaaaatggca
    13201 caaaaaacaa caactgcaac tgcagttgag gaaaagaaaa aagaaggttt ttggtccaca
    13261 aaaatcaccg gcgtaagttt gccactttat ttgatcatgg tccttgtcct gatcattgca
    13321 atggctttag ggaaattacc gacgaatatg attggaccaa tcgcagtttt ggttattttc
    13381 ggtaatttat ttcattttat cgggacaaaa atccctatcg tcaaaagtta tttgggtggt
    13441 ggttccgttt ttgcgatctt cgcttctgct gcgattgcta catttgggat cttacctgaa
    13501 tatgtagtga aatccacaga aaactttgtc agcaatatgg gcttttggga cttttatatt
    13561 gctgccttga ttactggtag catccttggt atgaaccgta atctattgat gaaagcttct
    13621 gttcgtttta ttcccgttgc acttatttca atggtcgtct ccttttttgc cgtaggactc
    13681 gtgggcatgc ttatcggaaa tggttttgca aattccgttt tatacatctc attacctgcg
    13741 atggctggtg gtgttggagc cggagccgtt cctttatcaa cgatttatgc aaatgtttta
    13801 ggtacggatg cttcttctat catttcccgc ctgattcctg cgacagcaat gacaaatgtc
    13861 ttagctatca tcggtgcagc attagttgct cgtgctggac aatcaatgcc aaaattcaac
    13921 ggcaatggta aattaatgga aaaaggcgat cttggcgatg gaaaacctcg aactgtgaaa
    13981 ccttccattc cacagttagg tgttggtttg atggtatcat tgactttctt catcttagga
    14041 caaattggta actattttgt tcctaaagtc catgcttacg catttatgat tattatcgtt
    14101 gtcatctgta aaatcgccaa cctattacca gagtattatg aagatgcagc tatcatgttc
    14161 aataacttga ttgtcaaaaa cttgacagca gctgttcttg ccggtatcgg tattgcgttg
    14221 ctgaatctga atgtattagc ttctgctttg acatggcaat ttgttgtctt atgtctgaca
    14281 agtgttatcg cgatttcagt tgtttcagga tttgtcggtc gtttgtttgg tttatatcca
    14341 attgaatcaa ctattactgc tggcttatgc aacaactcga tgggcggaac aggaaatgta
    14401 gccgtcttat ctgcagctaa tcggatggaa cttattgctt ttgctcaaat gggcaatcga
    14461 cttggtggtg ccatcgtttt gatcatctct ggtttcttga tgcaattact ttcttagtca
    14521 aaaaaacaaa aatacctgat catcaagtat cggcttggtg atcgggtatt tttttgataa
    14581 agatttacag gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgtgtt
    14641 cataaaattg gtgtgcttct actctgtatt ctgcagaatt aagccttatt gtcgttattc
    14701 ctcgtttttt cgcttcttct tctacgccag tcatcaaagc ctttccgatg cctctttttt
    14761 cagcatattt agacacagca agtgccataa tattgaacat tggatcgcta aaaatcgatt
    14821 cataggactc cgcttgtaca tagccagctg cttttttaga agtaggatct tcataaacca
    14881 agaaaaaatt tttctctggc tccttcagca gtttcttcat ttgtctcacc gtaacggtta
    14941 atggtatatc ataccctaat tgatccgcat tgatttcacg tagtgcttct gcatcttcta
    15001 aacgcatttc ccgtaacatc caaatcacca gccaatttct gattttttaa aatgaatttt
    15061 tttatcatag tatactcttt ttttcattta gaacaatatg tttcagtgat tttgacttgc
    15121 gttagtctag attctcctaa tgtttgtaag atgacaaagg cattgtaaca attgaccaaa
    15181 agaaccacga ggacaattac ttgaaaaata aaggtcactt tttttggaga caaaaccctg
    15241 ctaaatcttg ctccccacaa acctccaata acagcagcgg ggataacata aaacaacata
    15301 gataaatcaa agcgcataaa gctacttgtt aaagcaatcg ttaccaattt tgccagttga
    15361 gaaaagaaaa tcgtagatag agaatatagt gttgcttctt ttattggcaa tgcaaacatc
    15421 agcatcaaca aagaaacatt gactggtcca ccaccaatac ctaaaaaact agctaaaaag
    15481 cccagaaccc ccccacatat aaggtaccaa gtccatgacg tcaaatgaaa ttttggctga
    15541 tgatgcttgg tgtagaagaa cgcaaaaacc aaggtcaata ccgtcaaaaa aatttggatc
    15601 agctgcacat gcttctcgtt ttcaaataac tgtaaaaaaa cttcgaatac aatgttgccg
    15661 actactcctc caacaacagc tccactagat acccaaccga caatctgcca attcagtgat
    15721 ttcccgcttg atacttgtcg cacagtcgaa acaatggaca tcgtgaatac cgcaactgtt
    15781 gagtaaaaag aaatccctgc aacagaatgg gcgccgataa gatcaagaat cggtttgatc
    15841 aacacgcctc cacccatccc ggaaatagcg ccaactgtgt tcgccagaac gatcaccaca
    15901 aaataaatca gaccaatcat cttgtatttc ctttctcttg tttatatctt ttagcgattg
    15961 ttactctttt cttttattat acagtaaaag aaacaaacga ggcttcgaaa gcttttctac
    16021 tttttaagga aatttagtgc taaacgaaaa gaggttgtga aaaagctgtc ctgcatcaag
    16081 taataagaac agccaaacaa aaatagctcc tcataatttt cgtttggttg aacttattac
    16141 cgcagatgca agcttttgaa caccgtttaa gaaagaggtc gtgagcctgc cgttcagctc
    16201 cgagtcacaa ggaacaattt caaaaaacag cttctcatgt ttgttgaata ttgtgacttg
    16261 tgaccgagga gttggcagga gaacaccgtt taaggaagag actgtgaagt caacgttttg
    16321 acctgattat tagagaaaca aatggcaaaa tagttcttca tatttcgccg tttttcgagc
    16381 gaatgccgag gggcagctga ttgaacatcg tttatttgga aaaagggatt gtgacaagac
    16441 gtttgtcaca atcccttttt ttatatctta ttaattcatt cttttgttct attcttcttc
    16501 ttccgccatg aatgagaaac ccatatcaaa gaactataaa ggttatcaaa acagcgttta
    16561 tgactaaatg aattagagtt atctatcact atttttcaat cgatttaaac caaatggcat
    16621 acaatatggc atacaaaaac ttcaataagc cgtgggggtt tttaaatttc ttcttgttct
    16681 ttaaaatcaa tgagtggagc tacataattt gaatccactt tttcaaacgt cactttttgc
    16741 atcgacgaat gttttctttc ccaagataac gtaaatagat ttgactctgg acttataaag
    16801 tctacttcac cttctagtat tactttctta tgcaaatatt cctctaataa atatagactt
    16861 gctaaagcat acaacacatt ttccaaattt gcttccttat aatttgaaga acgaccatgt
    16921 tttactctgt tatatatttt ccaccaatct ggtgtatctt caggccaccc atctccaaaa
    16981 ggttttagtg aagtcaatgt taaagaattc ataaccctaa caccattatc caaacacatt
    17041 aattcgctag atttaataac agctttaaaa tcagttatgc ttttattgct actgctatca
    17101 aatccgcaaa gttctttgaa aataatctca cactccgaac caatcgctag tagtagtttt
    17161 tgatattcaa tagagaatgt tttgaaatta ttctcatcga gcatcacaaa atctgtggta
    17221 ttaataaact cattttctaa atgtagataa tacttccaat aaaaatttgt aaactctgct
    17281 ttgtccatta aatcacctct tttgaattat aacaaaaaaa acacgtccac aacagacgtg
    17341 ttctttgatt tctacacaat cagcttgcca aaaccatcca atcagtccta gcatgcctgt
    17401 attaaaattg aacgttcatc atctatcatc tttcttagtc cagcatgttt gcaaccacac
    17461 ctacaatttt cagtccaaca atagatttcg actagctaag tcttgcaaaa ttcaggcagc
    17521 taacccttca tttactacct taattatact aaatacccac aacgtttaca agtgtaatca
    17581 ttctaagaaa tctgtttagc tatgatcgtt gtataatctt taaacgccgt tccttcattg
    17641 attgaaataa ttttataact cttgtttttc caagctactt tcatatcgtt tgttagttca
    17701 taatcttgat cgtatcgaat aataaacgtc aacgttcctt caagaatggt acctacacta
    17761 gcaagcacgt cttttagcat ttgtgaacgc acattagccc aacattcaaa tacagtttct
    17821 tcactatcca ccatctgccc atcttcatct tttacccgtt ttgtcttttt aaacttgact
    17881 ctctcagtca attcatttac attcttaatg attggcataa taagcacctc tcatttgttg
    17941 tactaatgct tgtaccgtta ctggtatccg ttcgctgctg gcttcttgac ggttcaaata
    18001 ccaatgttga gtaagtaatg acaccgccaa atcaaattgc tgataattat ccatatcttt
    18061 atccgttgcg ctactatcaa tagcactttt tatatattcg cctgctgtat caatcaattg
    18121 ttgaataaag tcatcatcaa tagtgtgatc gatccgcata ctttttttta tgctatccaa
    18181 aattaccatt ttatctctcc taatgaaaaa aaggcagtgg gataaaccgc cgcccaatat
    18241 ttttctatac ttttggtgtt acaactggcg taaactcaat atacactgaa gcattttgat
    18301 caattttctt ataatcgttg cgaacgatca ctgcaagtcc ttgtgagtag taatcgaatt
    18361 tttcccattg agtcgttact tgatttctac gagcaacaaa gatagattgt gcaatatccc
    18421 ccataatcat agggaatgtt ccagctttag gattggcaaa caatgtatct gcaatcaata
    18481 ctactggcat accgaataat gatttaccac taggtgctgt cacatcgggt tgtaaaatgt
    18541 aacgtccatc tgaatctttc aatgtatcta ggtggtaata tccgctttgg tttagaatta
    18601 ccattttatt taatgctggg tctaatgcca cattgtatag ttgtttcaaa tcgtccaacg
    18661 tagcagccgt tttcttagtg aatgttttta ataaatccat gatatgctta ttgtcagtgt
    18721 tttctaccaa tttcgctaac tgatctttga cctcttgtac aatattcact gctgaatctt
    18781 ccacaacctc atttgataag gcaatcttac cagcacgagt ttctacttta taatcaactg
    18841 aagtaaacat ttcagcgtca atatcaccaa tttcagcaag ttcagctttt gttgctaaca
    18901 ctgcttgttg gttagttgct actggatatt taccttgtcc atttgatact gtttttactg
    18961 ttgcatattg agctaagtta taatttgaac gtttcaaatc aaagacttca ccgattactt
    19021 cttcgggaac aactgccgct gcatttacag tagtgactcc atcacgcact tcgccttgtg
    19081 aacggatata ttcttcatac ccacgcaatt ctgttttttc gttatcgatt aaagtttttt
    19141 ccatgttatt actctccttt tgtgctttag aagtggattc taagctattg tttgtttgtt
    19201 ttgcttggtt gttactcata aacgattcgt aagaacgctt atttacttgg acatttgatg
    19261 aatcataagc gggaacggtc acgacactaa tttcattaag tgctttgatt ttatctattg
    19321 atcgttcaat tgtgccatct tcttttttgt cgaatgaatc taatccaagc acgaacccaa
    19381 aggacataga atcaacaacc ccttttgaga tattctcata cacatcattt gaataggtcg
    19441 tgtcggttag ctctgcttca aaatgtaatc ctgtatcatc gacattcaat ttcaacgtac
    19501 ctgctttgac actagctagt ggctttgaat aatcgtgtcc atataataag aacacatttg
    19561 ataaatccac ctcttttaat gcttcgggtg taatcacttc tacaaagccg cctaaatctt
    19621 ttgacggttg cccgaatttc aacgcatagc ctgaaatagt tttgccgtct gttggttgtt
    19681 tttcattttc tttctctact ttgggagaat cggctgttag ctcagcttct tctgtcagcc
    19741 gtttttcctt ttcttcttca ttattcaact ggtgtaactc cttcctgttc ttttaattga
    19801 tatttctcca acgtatctaa atacgtaaag ttcaaacttg ctagtaaacg atcgccacca
    19861 tccatggggg gtagccccat tttcgaccgc ccctcattaa tcgtgagaag tgaccctttg
    19921 acctgatcca atacattttt tacttttgtt tcaggatcag tctctagtaa ctgatcggaa
    19981 ttaaagcgga tattggtttc aagtttcgta tctaattcac tcacaaacac attaaagtaa
    20041 tgaatcagcg tactttggat atactggaga ttactttgaa cggtgctaga atgttcattt
    20101 tcaacgccta aacgctcaat cggtacacca aacgccttgg caatttgttt tgtgttccaa
    20161 tcattggaat tgataagctt caaaacatcg gtgtttattt ctaatgtttt gtagtccatt
    20221 gtttcatcaa gaatgatcgt tctaagagca ttatctccac tacttgaacc gttggcttct
    20281 tcaaactttt cacgaattgc gttctttgct gatccgtcta aatctgactt atgaaccttc
    20341 aaaatacctg acccactgac accacgagta aagaaattgt gcaatgtgcg gttgccagct
    20401 tgttgaattt tcagttcatc acgtaacgca taaagtggtg gtaatcctgt taaaccatct
    20461 tgggtgaaat atttgaagtg caaaatatcg ctagacttca cacgtctttt tttatcacca
    20521 atctgataag acaacgtacc attatctaat tgagtaaccg ttacacttga gttaggtaat
    20581 aagtgaattt cttctacctt ttcaccgttg cgtttaatct ctgcaaagct attaccgttt
    20641 agcaacatat tgaccgccaa agcaaacttt aaatgccaac catccatttc tgaattaggt
    20701 ttctcgttta gtaacttcac cagttcatta tcagcttgtg gcatattgtt tttgaccaat
    20761 tgaatcggac ttgaagcaat atcactagca ataattcgaa cggctgcaaa cacatcacta
    20821 tttcttaatg cacgaacgct tgtaaagctg gtggtgtaat catccgattg gattgatacc
    20881 acatgttgta ggaaaggatc tcccgttgta ttttgtctcg ctttaaaaaa tgccatcttt
    20941 taacctccct tctgttctgt atttagcatg accgctaccc caataagcac tatgcccagc
    21001 gctaacaagc catagtagac atttgttagt aaggtgatag caaccaacac acacaccaat
    21061 ccaagaagta aaagaacggt atgaatgtaa ttagaaagaa aattcttcac ttgtataaaa
    21121 ttcattgtct gctttcgctc cttccgttgt ggtgaagtaa tccattgcaa acacataggc
    21181 attgattaat gcagctattg gatcaatttt gttactgttc tttgatttat taatctgaat
    21241 gccgttgtta tccgttttta aaatagcgtt gttcactgca tgggtgagaa tcgtattttt
    21301 attgtgaata acgttgcctt cataaacttg ttctctaaaa gttcgggtcg gaacgtttag
    21361 agtaatcgtt ccttgtctta cttctaacat tgggtagttg gctttttcag ctttactgat
    21421 aagcgaatta gcgttatacg ggtcataaca aatccccata cattccaaat cattttcttg
    21481 aatcaaatct tgaataaact gaaatacttg gtcatagtcc acgattccac tttctaattg
    21541 agtaatggaa cactcaccag ctttttctag ttctcgataa ggcaaaccgt cgcgcttttc
    21601 tttgtcttga agcccatatt tggtggctac aaagctatgt gaatcacaat aaagttcgcc
    21661 gttatctagc ggtacgatcc atgaaacgct agttaaatca tctgttttag ataaatccac
    21721 tccgatataa acgggcttgc ctgtaatatc ttgtggttct acttcggttg cctgccagtc
    21781 ttcagcaatc atatagctgt cctcactcgc ttgtctccat aaattgaagt ttttcaccaa
    21841 tacagaatta aggttatttt gtttcaaagc aaggttcaca tcatcttgaa tggttggaat
    21901 cattactttt ttgatttctt cactttcaaa aatcggattg gctttgatcc atttctcttg
    21961 atcgtgaatt tcttcgggat cgtccaattc ccatatcgct ataaagtacc tgtcggcttg
    22021 ttcttcgcct tttagcacac gatctaacaa taagtattcc tcgtacatag gaacgtttaa
    22081 atcaagccca gcggtactaa taacacacaa taagccgttc ttttgttggg tcattcctga
    22141 cttaatgacg ttgtacgttt tacgagtttt tgcttcgtgc cactcgtcaa gaattgcggt
    22201 tgttggtgca aacccatcta gcgtgctggt ttcactagcc agtgccatag cgaacgaatt
    22261 agagggcaag tgctgaatct tagagttcat aattttcagt tgtggtctta aaaacttact
    22321 agacttgacc acgtttctaa gtgaattaga cagcatatca tagcccaatt tagcttgttt
    22381 caaagcgtta cttacaaaca atacttgcct tgcttctgct ggttcttgtt ccataatcaa
    22441 cgcattggca gccatgccac ttgcgatata cgttttccca ttcttacggc tcatactggt
    22501 aaacgctcga ttgaatcgcc tgtaattccc ttctttagta cgccagccat aaagactgcc
    22561 tagaatgaat ttttgaaatc ctaacatctt gattgcttta ccgtccgtag atggtagcaa
    22621 ctcaatgaat ttaatcgctt tggtggcttg ttcttcatcg aacacataag ggaagctatc
    22681 tgacttagaa cgctccaaat cgcttatatg acgctcacac gcttggataa ttttcttggg
    22741 tgcaatcagc ttacctgata ggactttctc aatgtaatca ttcatgaatt catcaactct
    22801 ttgaacgggt cttcgggttc atcattttta accttgttca aagctagttt tgatcgtgat
    22861 tctaaggtca tacctagtga ggtggcttgt tttttcaaat cactcatagc ttgtgattga
    22921 acccgaaccg ctggattggc tttctttact ccgttttcgc atgtgataaa cgtacctgat
    22981 tttcttatct cggcttcggc tgttttcatt cgagcaaagg ctaaacaata ggctaccaat
    23041 gaaccataat ctgcttcact caatggataa tcttttttta atgttggtac gatacgattc
    23101 cattcacttc tagcgctcgc tgccatccag tcagggggcg tgatatttat taatggttgc
    23161 tgcttgaaca attcttctct agcgtccaca cggattgctt tttcttcact tgatatattg
    23221 ccttttgtat cgtgtaatat tttagggtgt cccatgttgt cctcctttca gacaaaagaa
    23281 aaaggaacgt aagcaattaa gctcacattc cttcgttttt cgatcagaat ttttctttat
    23341 ctaatttatc tatataaatg atttttccgt tctgcatttg aatggtcact tgaccaaaat
    23401 gaggtaactc aatggtgcgc tgtttccctg ttttatcagt aataacaatt gtatttttca
    23461 ataaatctcc tactttcatt atatactttt tcacttgtga ttacaagtgt aatcgttatt
    23521 tgtaattacc cttatgcctt ttcacaacgg ttatttgtac caaaatgagt aggcatcgat
    23581 tgtcagtaag tttcacacta tgccccctta actcaaaggg tactcttttc tcgtctttgt
    23641 ctttcattgg ctgtcttgga attatggtgt gcatggcata aagaacgaaa attagtttca
    23701 tctaaccgtc tattccaatc atcttttagt tcaattttgt gatctacaac atcagctttc
    23761 cttatcaagc cttcttctag gcaatcctca catactggat gatttaaccg atacaagtat
    23821 gaagcctttc gccatgttct actttgatag aattgaaaat atttaccttc gctttgcttt
    23881 ctttcttcac gcttaattgc cgtttgcttt gctttatgct tcccacaata cttctgccta
    23941 taatcaatca atgttttaca tccagcatga ttacattgtt ttttaactgc catagattac
    24001 caaccaaagc gatctcgttc gtcagaatca tcttgttccc attttgattt gtattcaata
    24061 taaggcgaaa ctatcttcaa gcacgttccg tctttaaaat taactttagt gattttatct
    24121 tcctcaaaac taataaagtc tacatcttct tgagtgtttg aatactcaat gtgtttctca
    24181 ccatcgaagt atctaatact ttccatatgc ataattattc tacctccaat aaattagtta
    24241 tcaattcatc atctgttagt agcaaagcca agaacttatc atggatgttt aaaaacaaat
    24301 tatcctcatt cgatgtaagc tctcgttgtc tcgctcgatc atataggtta agcagacaat
    24361 taaattcatc cattagctca cctgtatcat catagttgcc actatctacc aatgatccca
    24421 ctactagcga ttgacaccac gctactgact cctgcagcca atcaggttct tcttccatta
    24481 tctttaatac ttctggatca aatgtggaat aatcttctgt taccaatccc acactaaatg
    24541 ccagcgtgtt cattagaaac cgatccattt catctgtata ttgaaagttc ggtgctttgt
    24601 ttgttgaaat aatattgagc atttactttc ctccttgatt cgtaacacgt ggtaacacct
    24661 acgtaacagg tacccgttac cactcaatcc caacggggac aagggttcta gcgttttggt
    24721 aacaggtaac gggtaaaatc cagtaacatt attccccagc atattagcta actatttcaa
    24781 aagttttaag gaaataaggt gttaccccgt taccaaattg ctgtatccct tggggcagta
    24841 agggtaaact agtaacgggc taggtgttac tgacccgtta ctacccgtta ctttttgtac
    24901 tttccagctc gatttttatt ttcataggca tttgacttat tgtaaaatcc tcgtcgttta
    24961 tttctttttg gcagttgctt ccgttcccaa ccaggaatac tgctaataat caaggcaatc
    25021 tttttagttt ctgcatctga tcgaccattc aataaatcct ttgcttgttt atcaaaaaca
    25081 gcttccaaaa tatcagcggt gagtacgcta tcaactaaat aaaactcttg ttgcatagta
    25141 gctccattat agaaatgctg taaatttttc tcattacatt tactgtcagg atagttctga
    25201 taataagaac gtctcgccca cgctggagct tcttcccatt tatacgggat ttccatttcc
    25261 aaatatttag tgattgcttc tttaattgga ttctccatct tggcatcctc ttgatagtct
    25321 tttgcaattt ctaagacttc tttatcttca tcgggactaa aatagattcg ttgtccttta
    25381 tcaaaaagca cctttgcttc agctaacact tgaagaaaat attcatcctt tgttttaaac
    25441 ggattcatct ttggcttatt cttacaaggc aaaggataaa aacggcggtt tcctgtatca
    25501 tctttcaaat actgcccatc attcgacgtt ccaataaata cacattgcct tgcccaagct
    25561 atgacattcc gaccataagg aggtctaatt ttatcgatct tggcactgat gaagtttttt
    25621 atctgttcaa ttttagtttt gttcattgaa gataattctc caagttcaat aattacattg
    25681 ccttgaagct gcatataatc atctttcttt tcacctagcg aatctaatga atcggtgaaa
    25741 aaatcatccg tgtaaagtaa tgctggaacg gtactcttac caatcccttg cggtccatcc
    25801 aaaactggaa cgatctcaaa ctttattcct ggtttataga tacgtgcgac tgatccgact
    25861 atccaacgtt ttgttacttc tcgtgtgtaa aggttgtcgt ctacccctaa aaggtcaata
    25921 aacagtgttt ccagtctagg ctgttgatcc catttaactt tttcgatacg ttcttttact
    25981 gggttgaaaa cattctttct agccacattt actactgctt tgtgtatttt gttgtcatca
    26041 aaaactacat tgtagttttc ttcaatatat gccgttaagg catcctcaaa actgtcttcc
    26101 cattctccag cggtacgatt gatccaagga gaatctttta ataggtgaat gtatccgcta
    26161 aattcgttat aagcaattgt tccagctaaa ttgctatcat tggttaaaat caattctgca
    26221 ttcacaacac tgttactttt aatctgatcg tctttattgc atcttaatcg tgacatccag
    26281 gttttcaatt caatgatatt gtcttcacgc tcgctggctt cgggaaagtc taaaatacta
    26341 tctaatttat gcttatgagc catgagcttt ccccctcaaa tcttttttaa tcatcgatcg
    26401 aataatttga ttcacttctt tatcttttag cggtggttca ttgtacgcat ttgctgtata
    26461 aatcaattct gctaaagttt gattatctac accgtgccat agtaaccaac cgcataactt
    26521 agttaaatga tcgtttctac taccttcgcc agcgccttga acaatcatat ctaatttttt
    26581 tgctgtccct gtttttgcta cgggtcggta ttgagtcttt atttcttgcg gtttttttag
    26641 cagtaggttc actagccaat cgggagctgg tgcaatatta tttagagata cgttttcaat
    26701 aggttcatat gctctcccaa cacttcttga aataatcata gttggcgcta ttggaactcc
    26761 tttagtgata atatcaatcc cgcttaaact tttaccttta tcatcacgcc cgaagatatc
    26821 agcaaatgca cctgtttctt gttggataat tacaccatca ggaattttaa agaagaaatg
    26881 gattccttta cctggtgtga tttccgtata agtagaagga aatggatcat acattgaagc
    26941 aagtttttta taattctcaa cgccgtttcg tgtttcagaa tgttgatcta ggtcaactac
    27001 caccatttga tgatcttcaa gcattaaccc aatatttcta gttgcatctt ccgtccacca
    27061 ttcattaatt tgattagaat cgttagttgc atcattccaa ccactagagc cttttattgg
    27121 aattttagtt ccaggagata agggataaat tgaccatccc gacttttgta attccgttgc
    27181 tgcttgataa ttatttgcca ataaacctct cctccttttt caaaatactg gtataaatca
    27241 ttcagacacc tccatgagct tcaactgctc aagttcgtat ttcaacccat cccgaacaaa
    27301 ttcagcacct tttaattgat tctgatactt tacccatgta ttaaatgttt tcacccttgc
    27361 ttcttgcata tcagtaatgt ttaaccatgc ggttaaaatc tgatttttta gctcgttgat
    27421 ttctcgacaa attattttgt gttctgcatg ttttttgagt acatacaaaa ctgttagttt
    27481 ttcattgaat gaatttccaa agtaggtttt cccgatatta ttttctagtt cagcgatccc
    27541 ttgattaatt tcctctaatt caaggctatc gagttggttt aatagttttg ttttgtacat
    27601 ctacttatgc cctccagttc ttaattgata gtcgtactcg tcataagcag tgaagagata
    27661 taacgctaat cctgcaaaga tagctatttt tgcaattaat ggtaataagc cgaaaattcc
    27721 gccgattaaa atgcttccta taattaagtt tcgtttcata atggttccct ccgatttttt
    27781 aatatggtat aatcggaaca aagaatatcg ggttttatcc caattaagtt gcctttatcc
    27841 tgtccgccaa gactgttatg gataaggctt tttctttgtt ctcgatcatg ttaatgctcc
    27901 taagttgtct gcaattcagc agaacgatta taaattttct cactcactga aaactgcaac
    27961 ccatattttt ctttaacagt cattaattct actgtctcat ctaaaatagg ttcacgatca
    28021 accaacattt caggagtcat atctttcttt tttaccattt tggaatgacc ataaatagtt
    28081 gatacagctt tattggctat cacattcgct ttaatgtagt ctactggttt tggttcagta
    28141 atagcgtggc ttaatttagt cattgcttct ttttggtgtt ctttgtctag catgcggaat
    28201 acttgaaagc cttcaagtcc tgtggactgg cgtagttctt tgatgatgtc gaaaatccag
    28261 tcttgaaatg cctcggcttc ttttttacgg cttttgaatg ccatgcggta aatatttttt
    28321 ttcgttgatg atgtttacat cttgtgttct gcccaatgaa tcgatgacct tactagtagt
    28381 aaccccatct ttaggcaaag agtgaatggc tctagtaacc tgtttaagac ctaacgcttc
    28441 cgcaacatct ttcgccactg cccaccattc atcattaata tcgacaaatc ttataatgtg
    28501 tccgttccaa atttcagttt tcattcaacg tcctccagtt caagccctag caagctcgct
    28561 aacaactcaa cttgctgata caaaatttct tgatactctt caatagaaag tcgttcttta
    28621 aactctgctt gtactaaatc gttcaagcca cgcaatactc cgcttaccgt tggttcttgt
    28681 ggtagcaatt tttgatagtc catttgttcg tacaaacatt caaacgtttc ttctagcgct
    28741 ttgccttcca agtttgctaa ttctgccgct gtgtcttctg taagaattgt tgataatact
    28801 ttttcgattg ctttattttg tgtcgattcc attattattt cctcctatta atccctttgc
    28861 ccccacggct tgcgtttatt tttggtattc ttccataaag cgatcacatt catcttgtga
    28921 aattcttacg actccatcaa ctataatcaa ctttaaaccg ttaggaacat atttctgcat
    28981 catagtattg tatgaaacgt tcatatactt agcagcttgt gccttgttca tatattttgg
    29041 aaactggtac tttttttcta ataatgaaag taacgttcct tgagtttgct ctacttgttg
    29101 aacaactcga tcgtcaataa ttttaaaagc tggatcatca agcgtgagtt tgtcaatttt
    29161 taattccata tttatccccc ctcgttagca tacatttcta tacaaagatt ttaaaaaaat
    29221 gcaagaggct cttgaataca tttctatacc tcttataaac tcaatatacc atcatacctt
    29281 ttcatttgca agaattttaa catacttttt tgaactttta tgtttcacac atatttaaat
    29341 cgtgatatag tagagaaaat atatgaatgg tggtgaaaat gtggctagaa aaaaataccc
    29401 cgaagcagtg gagacaggac aaagaattag actggctaga attgaaaagg gactctcaat
    29461 ggaacaactt ggaggtaaat tatccccacc ggccagtaaa ggtgccgtta gtaattggga
    29521 aaatggctat aatcttccca ataacaccag gcttaaacaa ctttcaaagg tattagatgt
    29581 atccactacc tatctattaa acggatctta tacattaaga gatatgcata tgatgcccga
    29641 aattaagcga aatgtgcttg aaaagcaatt gaaaaaaact attgaaaaaa ataactctat
    29701 aattgattct aatctttcta atgctatcaa taacatcgaa gaagataacc tgactgtctc
    29761 acagaagatt tatttaacaa acgcaataaa atatcttaaa acaggatctc gaattgaatt
    29821 tcctgacggg agtaccccaa ttgcttatct cgcagctctt ctaaatacgc ttttacgata
    29881 tgactcatta attaatgatt cagaaaaaaa tacagatgag aaaaaagagt atcaagaatt
    29941 ttatgaaaat gatcttctaa aagagttcac agctcttgtt actgagctaa ataaacacta
    30001 ttcaaaataa taataaactg cctcctccaa ccgcccccac ggcttgctac actatggagg
    30061 gaaaactatt atggcaacat tcaagcaata tgaaactaaa aaaggtaaat tttggctcta
    30121 tgaagcctat cttggaatga acaaaatgac tggcaaacct gataaggttc gtagacgagg
    30181 ttacaaaaca aaaaaagaag cccagcttgc cttgtcacgc ttgcaagtgg actacgataa
    30241 aaacggtatt aaaaaagcga acaacgaaac gtttaaagaa gtttatgact tgtggctaga
    30301 aacatataaa accactgtcc gagaagccac cttcatgaaa actgaaatca agtttagaaa
    30361 gtggattctt ccaaaatatg gaaatcttcg tgtgaatgaa gtcacggtaa aaaaagccca
    30421 acaaattgta aatagctggg ctgaaactac tgaccaatac aaggtacttc attcaacttc
    30481 taagcgtatt tttagctacg ctatcaatct tgggattatt gagactaatc cactagacaa
    30541 cgtattaatg cctaaaagaa ccaccactaa gcataaagaa aaggtcaaag tttacagtaa
    30601 agaacaactg atcaccttgt ttgattacct aaacagcaaa cctgaaactt atcgaaacga
    30661 ctttgataaa acgttactac gctttctctt ttatagcggg gttaggatca gtgaagccct
    30721 tgcattgaat tggtcagaca ttgattttca agaaaatacg gtcaccatca ataaaacact
    30781 aagccaatcc aaaaatggct ataaaatcag tgatcctaaa actgattgta gtgctggggt
    30841 tcttccacta gatgaaagaa caatttctat attaaaaaaa tggcagatta atcagcggaa
    30901 atatatgctg acacttggtg taactgatcc gactatgatt ttttgtggta tctataaaga
    30961 actaatcaca caccacgcta tttatgcaag attgattact atttgtgaaa aagctatggt
    31021 tccatttctc ggtgtgcatg tagcaagaca cactcatgct tcaatgctct tggacgctgg
    31081 agctactatg gtagaagttc aaactagatt acggcataca aaaattagtc agacgatcga
    31141 ttgttacggt cacttggcaa aagaaacgaa agaaaagact gttgaaaaat tagtacaaca
    31201 cttaaatatt gactagatgg catacaatat ggcatacagt aaaaaataaa aaaaggagaa
    31261 accctatcat atcaaggttt ctcctagctt tatagtctat tcttcttctt ccgccatgaa
    31321 tgtattgaat acttcttcaa tcatgtccca ttctgcgtct gtttcgattt gttctaactc
    31381 gccttctgtt ccatcttcgt tttcgatgta tgcataagct tgtaattcta catcttcatc
    31441 ttctgaaaca ccagcaggat ataaaagaac atagttacgt ccaaattctt cttgtccgtc
    31501 aactgttagt aggatttcat ataatgtttc atttccttgg tcatccacca atgtgatgtg
    31561 ttcgtgtcct tcatggtcgt gggaatggtt atgttctgtc ataagattac ctcttttctt
    31621 ttattattaa aataattgct tacctgagcc gtctaaataa ttttgtaaga tcatcacagc
    31681 tgctaattta tcgatgactt tttttcgttt ggctcttgaa gtatttgcct gctctactag
    31741 catacgttct gcctgaacag tggttaatcg ttcatcttga taatcaactg gcaaggagaa
    31801 tagttctttg atcttttctc cgtatgacat ggaagcttct gcccttggtc cgatggaatt
    31861 gttcatattt ttaggtaatc caactacaaa ccggtctact tcatattgtg ctactaattc
    31921 cttcaaccga tcaaagccaa actcttcttc tgcttcattg atccgaatga tctctactcc
    31981 ttgagctgtc cagccaaagg ggtcactgat agcaacacct actgtcttag aaccgacatc
    32041 caagcccatt actctcataa attcactcca tgatttgaga ggtagtcctt gacaattact
    32101 tccatgatct catcgcgttc gtgacggcga atcaaatttc gagcatcacg ataacgagga
    32161 atgtaggccg ggtctccaga aagcaagtag ccgacaatct gattgattgg attgtagcct
    32221 ttttcttcca aagcccgata aacaatcgct aatgtttcgc tcacttcttt tttgcgacta
    32281 tcgtcaaaac caaaacgtac ggtttcatct gtaaaaccca tgattctaca cctctttccc
    32341 gcaaggtaat tccagcttac cttctacaac cttattttac tcgaagaaag ggaaaactac
    32401 aaacaaatat tccaaacttc cgaataaatt aagaaacact atttgtatgt ttttttcctt
    32461 ttttctgtag ctttatcaaa agtactcttt tcataaagta tacgctatat ttctttgaat
    32521 gtatgttttt acacaatttt ttcataatat tcataaaaaa ttcgaatagc tgctactaat
    32581 tttttgtttt tgttgcttgt cctgtctcat aattgatcgt gtagccatct tgaacattgt
    32641 agataaagac attgaattca atttttttgt cttcaatgct ttctgcttcc aattgcactc
    32701 ccctggctac cagttcattc ccctcaaaat gaggagtgac acggtagcgt acatgatgat
    32761 ttgtttcttt gatatatgcc gcaatatcat tttcgtgagc aagcatgtat ggagcattca
    32821 acgaacgtgt ccctgtcatc aagtttttaa tgttgttatt ttgaccagtc agctggaaac
    32881 caattaaatg gctgcgatta tataaccatt gcccgtcact taatttcttt tgtttccaac
    32941 ctgtcgggtc aatatacaaa ggctctctct tttcagtagg gaacaaatct tttccaagca
    33001 tagcattggc ttgtcctact ctgttcaatt tatcaatatt tgaatagctt tcccatgttc
    33061 ctttggaaag gctaagatcc tcttttgtaa attccggctg attcccattg atttctattt
    33121 cttgatgttt tccatcatat tctggcacat ccgctacttg agaagcttgt ggtgtggaat
    33181 caaaaatagt tgtaatctgc tgaaagatat ctgaatcttt ttgtgtggtg gaaacaccga
    33241 atgcccctgc aatcacagca gcgacgatcc ccacgatcgc cttggttgtt tttttgctca
    33301 tttctttact cctttactaa cagctcccta aatcttaaca tatgttcccc tataaataaa
    33361 tactagaaag aaatagaaac agcaaaaaat aaaacctgtt ttaagatggc ttaaaaatac
    33421 aaaaaagaga cggattcctc gtctctaatc agtcaccact ctatctgtaa ggtatttatt
    33481 attattcaaa aattttcttc atactcaaga caatacactg atttcacaac cgcttggtaa
    33541 acggtgcgca aaagcttgca tctgcggtaa taagtccatc caagttaaaa acataagaag
    33601 ttgtttttgc ttggctgttc ttattacttg ttgcagagca gctttttcac aacctatttt
    33661 aagcgggtga cgggaatcga acccgcgaca tcagcttggg aagctgggat tttaccacta
    33721 aactacaccc gcaatacaaa aacagtatac gcctctttta ttccttcctc aagttttttt
    33781 attctttacg cttctggtat gttttgtagt aaaataaaac taatcaatca attattggag
    33841 aagatgctat gacgggaaaa gaattagatc tgttgctgga cacttgttta ctcgctggga
    33901 aaatcatgat ggaaagcaat gcggagatgt atcgggtaga agatacgatg agtcgaattg
    33961 ctttagcttc tggaaatttc cgtttggtca gctatgtcac tcaaaccggt ctctttattg
    34021 gtcttgatcg tacctcgacc atccgaatgg aacaaattac caaccgttcg attaatttag
    34081 aaaaagtagt aaaagtaaat gatctttcac gtaaatatgt tgcttcagag ctaacattag
    34141 aagaacttta ctgcgaactg caagcaatcg accgagaccg ttctttcttt cctgtctggt
    34201 tgcaaatcgt tagcgcggca gtgatcagtg gaacaattat ggtgttattt ggcggaaaga
    34261 tgaccgattt acctgtgact ttagtcattg gaggattggg atacgcagct tatctatata
    34321 gtttgaaatt ttttagaatc aaatttttat cggaattttt atcttcttta ttgattggca
    34381 tagccgctat ctacagcgta cgttttggct taggaagcaa tcaggatcta atcattatcg
    34441 gatgtgtgat gcctttagtt cctggagttc aaattacgaa cgctatccgt gatttgctag
    34501 ctggacatta tgtctcagga gtttctcgcg gaacagaagc gatgatgact gctacgatga
    34561 tcggttttgc gattgctttt attttccagc ttttttatta ggaaaggaac acaagtatgg
    34621 ggacgatatt tattcagttt tcttttagtt ttttagcgac tgcggcattt gccgttatta
    34681 caaatgtacc taggcgttca ttgatttgtt gtggtctttc tggtgctttt ggctggatga
    34741 tctactggat ctgtgttcaa ataggcggat ctgctgcttt tggttcattg cttggttctt
    34801 taggagtagc attcatcagc gatcttttct ctaaacgttt gaaaatgccc gtaacgattt
    34861 tcaatattcc cgggatggtt cctctagtcc ctggtggttt agcttatcaa gcggttcgaa
    34921 atctcgttac tgaatcctat cgagaagcgg ctcattatgc ggtccaagca attatgattg
    34981 ctggagcaat cgctttaggt cttgtattat cagaagtctt taaccataac atccgtaatt
    35041 tcaaagtaaa gcgtgacgca atcatgatga aacgaaaaaa acggcttttt aaataaacga
    35101 agtgtaacaa agaaaaggtt gtgaaagaga cgttcagcct tgagcgatga caaaatatct
    35161 cacaaacatt gcgacaagta accgcaggag caataagaag taattatgag tattaaaaaa
    35221 agttttgtaa aaatagcttc tcacattttt acataaaatt gacttaatac tgagaaactg
    35281 acttttgaac accctctatc tgtaaaaaag gggctgcgac atgatttttg tcacggcccc
    35341 tcttctttat tcaaattttt tccaaaacgt tcgttgataa taaacgatcc atagagcgga
    35401 aagtattaga actaaaccga tcacaggaat attcactaac caaaatggat aaaacaaagc
    35461 caacattccg tataagacga taagaataat actgatgatc aatgaaccca tcatagttgc
    35521 gactaaaccc aaacttcctg aacctctgtt aaataattgg ttaatattgg tccagttcag
    35581 cagcagcaac cgataatctc tagcgaaata acgtagtgac aataagtagc agccgaataa
    35641 agaaccaaat agcaagctag ggataaaaaa caaaggcagc tgaaaagaca gtccgcttaa
    35701 taatgcgatc ccaccagtta aaaaactctg caatatccat ccgaatctga acttttcttt
    35761 taaatattgg ttcatagaga ttggcaaaga acggataaac agaaaatttt cttgatccaa
    35821 agaaataaga ttgctgataa aagaagtctg attaactgtt aagaaagcca atgcgatccc
    35881 taccaggaaa acaacaccga ttaacttatt atctaaatca gttaatttta tttcgcctcc
    35941 tagggcaaaa gcaagaataa aaatgattgg agttaacaag gaagaagaaa gaacttgcat
    36001 aatcaaatta ggttctttta ctaattgagc attgtaacta aataaaagct gatgcacatt
    36061 ttggttcttt ttatgggttc tttttatttt gaactcactg tttgaatcag tcagttgctc
    36121 tgtaagcttt ggtactatca gctgttttac agcaaggaaa aatcccagag ccgtgatagc
    36181 tagtattccc caacccaata aaccttgttg agaaagtggc tgactggcag catcatagaa
    36241 tggcaataat atcgaaatcg gatgccgatc tgttaactca ttttcaatag tccccgtttg
    36301 atgattcatc cacatgattc cagcaaatgc gatcagcatc gagctgccaa gcaagatact
    36361 agaaactgtt tttctatgtt ttttaaacac tgctgttcga gccaaaccaa aaacaatcaa
    36421 actagccaga caaaaaagaa taatcaaaaa tagagaaaac aaaaggattg agataatgat
    36481 agtaagcaaa atccctagat cagctcgcca tccggttaaa ataaacatga ctagaagcgg
    36541 aaaaacaaaa ggaatgatgg tcaaagcaac taccaaaatc tttgctgaaa aaacctgttc
    36601 ttgtccaaaa ggcagaggca aaaatacttg caggtcattt ccttcaaaaa agatattata
    36661 aatgactgat atactttgag ttaaacctaa cataccaaaa agtgccacgt aataagtaaa
    36721 gaaacctggc aatttaacaa agttcatagc aaacatagca aatccgtaaa tcagtaaaaa
    36781 aatcagccct gaaaaaagat tttgagacaa aagatagata gttaatcgct ttcccttttt
    36841 tcctttctct cttgctttat tcgtgagctg tggattaaca tataaaagat taactcgaat
    36901 taattctaat aattgtcgct tatacatacg gtccatctcc tgtcgcttgc cttccagcca
    36961 ttttcaaata aatcttttct agcgtttcat caggtgagcc attcaataat tcactaactg
    37021 ccccctgata aatcaattca cctttcttca aaatagccag tttatcgcat aactgttgtg
    37081 cggtatccaa tacatgtgta gaaaaaatga ctgtttttcc ttttgcggca tgagccttca
    37141 tcatttcttt caaatcaaat gctgcttgag gatcaagtcc ttgcaaaggt tcatccaata
    37201 cccaaatatc tggatctggc agcaacgtgc cgatcaaaat cgttttttga cgcattccat
    37261 gtgaaaaact tgcgattgtt tcattttgat gactatacat atcaaaaagc gctgtcaatt
    37321 ctgctaaacg cttttctttt ttttgtgtgt ctaattcata ggcagctgaa atcaagtccc
    37381 aatattctcc agccgtcaat tgtaaaaaaa tatctgggga atcaggcaca taaccgattt
    37441 tttgtttgat actcaatcga ttttccgtca atttcattcc atctactaaa atcgttcccg
    37501 atgatggttc aataatacta actaaacttt taatcgtagt tgattttccg gcaccattat
    37561 gtcctaaaaa tccaaaaatt tccccttgtc ttatcgagag agacaagtct tttaatgctt
    37621 gttttttccc gtaactttta cttacattga tgaattcaat catttctgta ctccttttgt
    37681 ttgttattac tttttattgt acccttcgtt attggtggag taaatgtcaa aaagagtgca
    37741 taacaaaaaa ggatagaaac gactaggctg ctacttgggt tgttctcttc tacaggaaaa
    37801 cactcttcta acaggtaaga tatgagtctt tgttttcttt aaaatgattc attttctatc
    37861 ttttcttgca taaaattctt tagtttatca atacttctct ttcccatctc tagttgttct
    37921 ccatttgcaa atgttactat ttcgtttact ctgctaatag atgtaatgtt ttcagtatta
    37981 atgatataag cttttaaatc tttacataaa tactcatatt cgagtgattt tttatatttt
    38041 ttaattgtgc cgtttacgag aatctcttca tctttcaata ctaggcctat tttattttta
    38101 taacctggca tgctcattag ataaaggatt tcatcgtaat ggatatagtg atcgctatta
    38161 ccataacgga cagttgtcca cctttttttc gtagcatgtt gaacaggata gttttctttt
    38221 tctttcaata agtaactttg gatgcttttt ttattttttt cataagtgaa ttcttttatc
    38281 aaataaccac taggcgtaat ctttcgatta ataacagtta ttgctttatt cggatgagac
    38341 gttagataaa caattttaca cttttcattc ttttctctaa gcatttcgcc tagttcgata
    38401 ccgctataat aggcgttcaa atcgatgtct aggaaaaaaa tatctgagga atagatttca
    38461 ttatttttaa tgaattcaac aaattgtata acgttcaatc cctgaacgat ctcaaatttc
    38521 caaggactta ataaaattct ggattcttcc aaaatttcta ctagcatttt ttcaaaaacc
    38581 aaactatcat ccaaaataaa tatacgcgtt accataaaaa gtacctccac tcgatatttt
    38641 actatcaaaa tcatttcata caacttttca acatacatgt taggaataaa ttgtaattct
    38701 cttcacaatc ttttccaaca cctttgaaac cgatatcaaa tactttcagg atgctgcttc
    38761 taaaataaat atcaaacaaa tgaatacatc atgcaaaaaa agccagacaa ccgacgtcct
    38821 tgcgtgtcag ctgtctgagc tatgtttatt tattttgttc ttttaaaaag aacgatacac
    38881 atactgattt ttcggagcag agactttctc agcgtttgta acaactacag atgggatttc
    38941 acgcttgaat ggttgataat agtctgattg gatggttcct tctattttat accattcatc
    39001 atttttaacc gttactcctt ccggcatatg cgtcaacaaa ccaaagactc ctgaatctgc
    39061 cacacaatgg ataatgccga atcggaacaa gaagatatct gtattgctat cgttcggtgt
    39121 ttggtaaaca aatccttcat agctgatttt tttgccaatg aattcgctag ggtaattata
    39181 aattagctcc ataacttcta aatagttttc atctgtgatc ttgatagttt gcttatcccc
    39241 atacttttct ttcaactcat tcatttggtc taagtagtct gatttgttga aataaatact
    39301 ggtatccggc tttaagtatt gtgtctgcat ctccggatcc cctactgatt ctttacttac
    39361 tgggaaatta aatcctttgg cttctacgat cgtagtgtcg agactaactg ttggaaacaa
    39421 cgtccccacg attactggca aagctagcag tacataagcg attccacgtt gataaggttt
    39481 tgacaaccca tggtcatgat cgtgctcgtg tgccttttct tctgcttttc cgtttttgac
    39541 ccacaaatac agctgaacta aagccaaaag agcagataaa atcattgaaa gaatagctaa
    39601 gtagcgataa tggacattga tatattgatc gagtttgcca gaaacttgca agtacatcat
    39661 caattcgaaa tacccagata aaatcaaaaa ccgcagcata ttttccctcc taaataaata
    39721 atgtgaatcc aataacgcta agggcaatca gtccgatcag cgataaaata aagcgtcctg
    39781 taaaatatcg tttcatcatc agtagatttt tgatatcgac cattgggcca aagaccaaaa
    39841 aggcagctac aggtgctgtg ccgaacagac ttaacaaaga agatcctata aacgcatctg
    39901 cttctgagca aagagacatc gtaaaggcca ataacagcat gatgataatg gctaaaatct
    39961 ttgttgagcc gagcgttagc ataattctag ttggtaaata agtctgcatc gcagctgcga
    40021 ttaatccgcc aatgatcaaa taacgacctg tatcaaaaaa ttcgtcgatt gaatgtgtca
    40081 acacatgccc agtttgatgg atcagctgat tttttggttc attcttctca ctttgttctt
    40141 cttcatggac atgatgatgg tgattttctg ctactttaga cgtgattttt tctttcaata
    40201 cagcgtcttt ttgaaaatag gcaagccaga caccaatcac aagggcaaca aaaaagctgc
    40261 ctatcacccg ccagcttgcc atctgccaag aattgccaaa tgcgataaaa gtggaaaaaa
    40321 tcacgattgg attgatgatc ggagcagtca gcataaaagc aaacgctgtg tgaactggta
    40381 cgcctttttt aacaaattga tgaacgatcg gtacgatgcc gcattcacat gaaggaaaga
    40441 aaaatcctaa gatcgaacca gtaaaaatag agaagatacg attttcagga agccatctct
    40501 tcacacgttc tggggtcaaa aatacttgta aggcgcctga gataagacag cctaataata
    40561 cgaaaggtaa tgcttcaata acgatcgata agaaaatcgt actcatctgc aaaaccgaat
    40621 ttggaataaa agaaaacatg agtttcctcc tttttatttc tagttcattc gctttgcaaa
    40681 tatacgcttt tattcaaaaa aaacaagttg ctctgtaaat ttttacacaa ctcttatttt
    40741 ttaccaaagc acttcaattg actttttctg ttattcccat taaattaagg tctcatacat
    40801 atcacttcta tcataaaagg gagaataaaa atgaaacaaa ccactaactt gactgaagtt
    40861 caagacatac tgcaacagtc atccgttgcc gtcatttatc tttcgatgcc taattgcagt
    40921 gtttgtcacg ctgttcgtcc acgactagag gaacttctca cacattatga tattccggca
    40981 cttcatttgg atgcatttga aacaccagaa gtagctagta aatttgaagt gctgactgcg
    41041 ccagttgtcc tgatttttca tcaaggaaaa gaagtctttc ggcaagcacg gttcatcgac
    41101 tttaaaaaaa tccgttatct actagatgaa ctgacagcag aagatgattc cctttcttat
    41161 gaagaaatct tccgaacaaa ataatcgttg tccaaacaga aaaaagcgat tgaacaagtg
    41221 aaagttttgc cttcatttgt tcaatcgctt tttattttta ttctgctgta ttttcggaat
    41281 gcgcatttgc gtcagaatgc aagaaataga tcaaggtttg caattcattc gtcaaatcaa
    41341 cattttggat caatacatct gacggagcat tcaatcgagc agcggtaaag ttcaagattc
    41401 ctttgattcc tgcttccgtt aactgattga ctacttcttg tgctctttca gcgggtatcg
    41461 ttaaaattgc tacttcgatt tgttggatct tgatttgttc tgacatatct tccattggat
    41521 aaacggggat accgtctacg atgcgcccaa cgatttcctc attcacatca aaagcacaac
    41581 tgatacgtat gctattactt tgatggaatt tgtattttag taaagcactt cccaagttcc
    41641 ctacaccgat cagcgcaaca tttgtcaatt gatcgtcgtt taatgtcttt gcaaaaaagt
    41701 gcataagatc ttctacatca taaccatacc cacgttttcc taattcgcca aaataagaaa
    41761 aatctcgacg gatcgttgca ctatctactt gaacggcttc acttaattca gttgaagaca
    41821 cttttttctt tccagcatta tttaaaatcc ttaaatagcg ataatacaaa ggcagtcttt
    41881 tagctgtcgc ctttggaata atatgatctt tcacgaaatg acctccacgt attgtctaat
    41941 tacatctttg atttctccat catagcagta tttataggaa aaaatcaatt ccattgctca
    42001 gatattgtga aattttattc tttattcatt tatttatcat ttctgcaaat ctcgcagtat
    42061 aatggaattt atggtagact actaattgaa aaatagtttt ttatttgagg agaattcaca
    42121 aagtagaaaa gaggattcca tgatattatt acaagccaat cagattgctc gccattttgg
    42181 tgcagaagtc ttatttgaaa acatacattt agaaatcagt acaggcgctc gtgtcgcttt
    42241 agttggcaga aatggcgctg gaaaatcgac tttattaaag atcattgctg gaattgaagc
    42301 acctgatgaa ggaaagatcg ctaaaaataa aacagcgact ctcggatacc tcgctcagga
    42361 cacaggactt gattccaatg aaacagtttg gaacgagatg ctgaaagctt ttgaacaagt
    42421 tagacaaatg gaagagcgga tgcgcgagct agagattttc attagcgaag gtacgccaga
    42481 cacacctgct tatagttctt atttaaaaga atacgaccgg ctgcaacacg atttttcaga
    42541 tcgaaacggc tatggttatg aaaatgaaat tcgttccgta ttgcatgggt ttaaattcga
    42601 ttcttcattt tatgatcaga aaatcgatac tttatcaggc ggacagcgca cacgtttagc
    42661 tttagctcgc atgcttttgc aacaccctga cattttgatt ctagatgaac caacgaacca
    42721 tttagatatc gagacacttt cctggcttga gaattatctg caaagctata ctggtgcatt
    42781 attgattgtt tcccatgacc gttatttttt agataaagta gtcaatgaag tctatgaatt
    42841 aagcagaaga aaaatgcgcc attacaaagg taactattcg aaatatctag atttgaaagc
    42901 agaacagtta gcaagtgaat ggaaggctta tgaaaagcag caaacagaaa tccataaatt
    42961 agaagacttc gttgctcgga accttgttcg cgcctctacc acaaaacgag cgcaaagccg
    43021 cagaaagaca ttagaaaaaa tggaccggct ggatcgtcct caaggggatg aaaaacaagc
    43081 aaactttcta ttcagtatag aaaagccttc tggaaatgta gtcttgcaaa tcgaagatgc
    43141 cgctatcgga tatgataatg tcaccttatc tgaaccaatc aatctagata tccgtcgtca
    43201 agaagcgatt gctctagtag gtccgaatgg tatcgggaaa tcgacgttgc tgaaatcaat
    43261 cattcaccag atccctttta tccgtggaaa agaaacgcta gggacaaatg tcaccatcgg
    43321 ctattacgat caaggtcaag ctgatctgca tacaaacaag acgatcctag cagaattgtg
    43381 ggatgagcat cctaccactc cagaaaaaga gatccgaagt gctttaggtg ctttcttatt
    43441 ttctggcgaa gatgtagaaa aaccaatccc tctgctaagt ggcggtgaaa aagcacgggt
    43501 tgctttagcc aagttagcga tgaacaaaga aaattttttg attttggatg aaccaacaaa
    43561 ccatttagat attgacaaca aagaagtatt agaaaatgca ttgatcgatt atgacggcac
    43621 actgcttttt gtttcccatg accgatattt catcaatcgg atcgcgacaa aagtcgtcga
    43681 actctctgaa aatggcagca aagtctatct aggagactat gattattatt tagagaaaaa
    43741 gcaggaagaa gaagaaatcg ccgaattgtt ggcacaagat gagccagaat ctgctcctgc
    43801 tcccacgaac aaatctgatt attaccaaag caaagaacag caaaagctta tccgtagcct
    43861 tcaacgaaaa attactcagg ttgaagaaga aatggcgcgg atcgatgaat ggatcgatca
    43921 gttggaacaa gaaatggtcg cgccagaaaa cctggaagat cacgtaaaac tcaatgagct
    43981 gaaccaagat ttagaagctg ctagacaaga tcaagaaaca aaattgacgg aatgggaaga
    44041 actaagcttg gaattagaag aatatgaaaa ttaacgaaaa aaccaaaaca gaaaccattc
    44101 atgctgtttt ggtttttctt tttttatttt atcaaacaaa aattccatat ttaccttttt
    44161 caccaataag aagaccaatt actagtcatt ctcactaaag cttctaaata atagtaatca
    44221 ccccaaatac aaggtgtatt taccccatta tttttgggct tattataaac agactgtgtt
    44281 aaaaagccat cttcttcttc cgttgtagca tatctttctt tcaattgtcg cataattttc
    44341 caaacagcag actggtataa gttcttttct gttttgtcta atagacctga ttctattaat
    44401 tctaataatc cacaaaccat gatcaccata ctagatgtat ctttttcttc tctgctagaa
    44461 tcatcaaaga tcaaatccca ataacaaatg gtatcttctg gcaaccgatt cataaaataa
    44521 ttggctaata ttttgcttaa atcaaaatat ttttgttctt tcaaataacg ataacttaat
    44581 gcaaaaccgt aaatccccca tgcttggcct ctagcccaac aggaattatc actataacct
    44641 tgatgcgtac ttccatatct tggttttcca tctttgacat ccatataaaa tgtatgataa
    44701 gttgaaccat cttctcttac taaataatct gctgcatttt gaatatgagc ctgagcagct
    44761 tttttatatt tttgatttcc agtttcttca ctcgcccaat acaataatgg aagattcatg
    44821 caacaatcca tgatcattct accttgttgt gaaggatcac taggatctcc ccaagcttgt
    44881 ataactcctg ccttctccca aaaacgctga agtaatgttt ctgcagcatc aattgaagct
    44941 tccttcattt ttcgatttcc cgttatttta taatctgcta cggttgataa agaatacaaa
    45001 aaaccaagat cgtgatgatt agtgataatt tgacagtcaa tcctgtggat aaaatctata
    45061 tctgcttttt cagctgtttg ttgaaataaa ggatcacccg tcttctcaaa taacaaccaa
    45121 ttaattcctg tccaaaatcc ttctgtccaa ccaatattgt ctcttaatgg ataacgtcct
    45181 tttataggac actcgcttgg gaattgatac tgactgttac tgatacgttt gattaacctc
    45241 tccgtgtgtc tgcgacaacg ctcgatctca ttattccatt ctttttccat taattcttta
    45301 taacattcat ttatccattt ttctggatgt attatctctt caaacatcac tttatcaagc
    45361 atttccccac cagcctttag cataatttta gtcttttttt attgttttta ttttggatag
    45421 ttagcccggt tgtcccatca aaatcaattt tgggacgatt gttccatact tctaaactat
    45481 aatcccgatc ttgatgagca accactcctg ttccgagcca atgggttcct tttccaaatg
    45541 taccctctaa tccaggaata aaagtgtttt cccatgtcat caagtttgta ttgggcaaac
    45601 ccataatagc tgtggatttt cgtttaatag tatcgtcact taaacagata atacctgaaa
    45661 agccagcttc actcacccta tagttttcct tgtcttcttc tatgattatt ccttctttct
    45721 caggagaaca ttcaatagca aaccctgtct catatagaac atattctgta tctaattcta
    45781 ctttgtgtac gcgaatctgc cagccttcaa aaggaataag ccaagttctg atttttgcct
    45841 gtaaagtagg ttcccatgta gaggcaagta cctctttctt acatacaatg tctcgtacat
    45901 tatttcgaac gatgaactgt ccataggttt tgatgctctc attcttaaaa ctggtcgtga
    45961 tatatgtgtc cgctcgctga atccctagca ttgaatccat tgccatttct gctttcaatt
    46021 gattcgctcg cggtatcgaa aaaccaaatt gactagaata agcaaatttt aaatattttt
    46081 cattggtcaa tgtatggaaa tttggcccta attgtcccgc attcaaaaaa aatgtatgtc
    46141 cattatcatg ttgtatgagc aaattggctt ctgaaagaag tttagttcct tcacgaatag
    46201 gcataggcat ttctttagcc gtccaatact catggtcttc aggtagcgcc aacaataaaa
    46261 aaatcttatt cagccataat ggagataacg tactattata gggttcagtc aatgtcaatt
    46321 gagggtaggt ataaccaata ttcaggatac ctttttcatc aaatatttct ttttgcatcc
    46381 accatcttat attccgattg actaacccct tgatttcacc taatttaaaa ggcaaaagat
    46441 cggcccacaa catagctgac caaaagcttg aaactgcaaa acgataaatc atgcttcgac
    46501 caaaaggcac atttgcacca tcttccgcaa aaaaatgaat atagtctcta gaaaaatcaa
    46561 tagctcggtt cttatatatc tggctaatgg gcttttctgg ttctagtacg ctataaatca
    46621 aaccataata atgaaatgca aaaggattat aataatcttg gcgacctctt gaactgtctc
    46681 gataccagcc attttgaaga taacaatttt ctatattatc aaatgcttct ttcagtgctg
    46741 attctttaac tggataacca aatttttgga taattgttcc gacaattatc ttaaaaaatt
    46801 gccaattacc atctgcacat tcatattcaa aaatttgcga gaaccaatga ataatctggc
    46861 ttttttctcc tgtatctaat tttttccata ataattgttc atgatgatat aacgctaata
    46921 acattgcggg catttctacc atacgctggt ctctttcatg tatttctccc caataatcgg
    46981 gatgggaagg atctgttcca gcttttattc ctgatagaat taatttcaac catcgttcat
    47041 ctaattggtc aattgcgggc cccgctcccc ataaaacccg agagaatcct tccattcctg
    47101 caattttatt tccatatcct accccttgat gtatataatc aatttgtgcg ccacttgctc
    47161 gataaaacgg ctggagtggt tgatttatct ttttaaataa gtcaaagtaa tcctgtctgg
    47221 tccttaatag tggtactgga tgtttcattg tattttctcc tctttttact accaaatagc
    47281 taattccgac tgttctaatc gaagtaagcc ttcaataaaa aaataatcag aatagatcag
    47341 tggcacatta taagtcagtt ctctatggta tgcttctgct ccgcctccaa tgatcccatc
    47401 ttcatccata ttccaatttg catagtgctt ttctgtcttt tggagtatat tgatagcagc
    47461 ttcatgatac atttttgatc gtacagagga aacatatcct gctaattcca gaagaccgca
    47521 tgcagcgcat actccagcac tggtatcaat attcgaatct tttacagcaa aatcaatgag
    47581 aggaatataa ccagtttttt cgattttctg aataaaattt actgctatct tttctgctat
    47641 agctagatag tacggatttt ttgtgtaacg ataacttaac gcgaatccat ataaagccca
    47701 tgcatttcct ctgctccaac aagaattctc attgtatcct tgcccgccaa gtaaccgaat
    47761 atatttccct gattctgcat caaattctgc gatatgcccg gttgatccat ctttcctcac
    47821 tagatactta gctgttgtat ctgcatgctg ccgagcaatt ttagagaacc tcggatcacc
    47881 agtttccctc gaagcccaat acagtaatgg taaattcatc atactatcaa taatcatcca
    47941 accaggttta tggtcattcc atgctactaa atagttccct gaaatattat agcgtccagc
    48001 aagtaccgta gctgcatgta accctagttg tcgactatgt ttgttttgag actgttgata
    48061 atgtgcaata gccgatggta agaataaaaa acctacatca tgatgaacgt ctgaaaaatg
    48121 gtacagggca gattctaata attgttcttg ttgtattgct gcctctttga acttttctcg
    48181 ttttgtcttg tgaaagagct gccataaaat accactccaa aaaccattgg tccaccagtc
    48241 gattcctctg tcctttatta cgtctgtata ttttccgttg taagaaatat atggaatttt
    48301 attccccatt ctgtctaatt ccgcatctat cttttcagaa gctacattca aaatttttct
    48361 tgaccattcc ttcgtctgtt cattcatgga tatcccgcca ttcttttaat ttttatgaat
    48421 gtggagttgc tcctagaacg aactagatgg ttaacattcg ttattttttt tacttatttg
    48481 cagctccaat gtcattctcc ttaaatttag actgcgccca cacataaatc aagaccattg
    48541 ggatcactga agttgctaaa attagcataa ttgcgccata attaattggc tcacctgtac
    48601 tagaaaccgt catatttttg ataattaagg gaaccgtata tttatcttta tctgaaagca
    48661 tgactaatgg aaatagatat tgattccaac ttcccataaa gataagtaaa ccactagtaa
    48721 taatcgtagg tgataaaatc ggtacagcga ttttcgtaaa aatcacccac tcattggcac
    48781 catcgattct ggcagcctcc atcagtgctg taggaaattg actgctatat tgtcgcatca
    48841 taaacactga agttgtactg gcaatcgaag ggatgatcac acctgcataa gtattcgata
    48901 atcctaaacg actcataatg taaaataaag gaatcaatgt ggtaaaaaaa ggcaccatcc
    48961 tagatagcat aactgtattg aacaacagtt tattccccat aaaacgatac tttgctaatg
    49021 cataacccgc cattgtagaa ctggctgtag ctaaaactgt actgattaca gcaataacaa
    49081 tcgaattcca aataatcaag ggataattat atctcgccat gatggcttgc cagtttttta
    49141 atagcatatc cccaaacttg aaattgattc cgccacttaa aatttcttga ttactttgcg
    49201 ttgaagctac aatcatatat aaatatggca tcaaaaatat taaggcacca aacacaataa
    49261 tgacgtacgt aaaaattctg ctttgtatct tttgtttagt catttttatc acctccgaat
    49321 ttaaattgaa tagccgataa tataacagat acaaacacca tcgtccaggc aatagcagag
    49381 gcttttccaa aatcaaccat ttcaaaactt gttttatata aattgactcc tacgaccaaa
    49441 gctgaatttt ctggtccatt ggaagtccca aagaatatat ttgggatatt gaaattaccc
    49501 aagccattga tagtggctaa aactgtggcc attaaaatga ttggctttag catggggatc
    49561 gttaataaaa agaattgccg aaatttcgat gctccatcta tttcagcact ttcgtatatc
    49621 tctttagaaa tcccttgaag accagctaaa aataaaatag tgttatatcc tacactaagc
    49681 caaatcatcg tgatgatcat tgatattcgt atccaaaaag gatttgttaa ccactgtaca
    49741 ccgttaattc ctagcgcatt caaaaatcca ttgaataacc cagtcggatt gaaaaaagtc
    49801 atgaatacgg aagctacagc caccgttgat gtaatggttg gaatataata gacagttcta
    49861 aaaaatcctt ttccttttaa ctgtgctgaa ttcaataaaa cagcaaagta aatagctaat
    49921 agcatcgtta ttggaattga tcctgccatg ataatcaaaa cattcgtgta agatttaata
    49981 aacaatggat ctcggaagat agctagataa ttgtcaaatc caacaaattc aaatgagtag
    50041 ccaattttca taaaactaaa agcaaacgag ataatgatgg gaatcaaaat cgtcacaata
    50101 aagaaaataa caaaaggtgt gaccaatcca taagcacttt ttttatgaga cgtatctgtt
    50161 gcttttttct tgctgttctt tattgtcttg cttggtaacg aagattgcat catcgttaag
    50221 tcctcctaat attttttaat caccagttaa ttttacgccg tacttatgag caaaattttc
    50281 tgcttctttt agatatgaat tttcaaaatc acctgtttgc caatagttat atgcagcggt
    50341 agtcaaataa gacgtagcat ctgaataagt ggctggtaaa acagtatttg atgtattttc
    50401 attgatttta ttcaatagtt catagtattt ttgtcctcct tagtagtcaa actttttctc
    50461 agctgcttct gtttgatagg ccaatgagtt agatgtagcc acgcccagct ccagtgcgtt
    50521 ggctaagcat tcttcatctg tcagcataaa tgtaagaaat tgttgtgcca cttgtggatt
    50581 tttggcatct ttagggacat aaaaacttga tcctcctgat actgcacctt ttcctggatt
    50641 ttcttttgaa aaaggaatca gtggagccat gcgccatttg tcagatgcat caggaaaatt
    50701 caaagacata ttcgtagcat accagccgcc agcaacaaac attgcagatt cttgaaatgc
    50761 tttctcagca tcttgtgcac cgtagaaatg tacaatatct gagtcgatca tttctttgat
    50821 taactttgca gcatgtttcg cttctttagt attcagacgg atatttcctt cttcatcaag
    50881 caaagattga ttctgctggg ctagcatgtt gataaacaat tcttggtccc cagattctgg
    50941 caaggcaatt ccaaaactac cagttttttc ttttaatgct tttgccacct ggatatattc
    51001 ttcccaagaa ctgatttctt caaaatctac acctatttca gcaaattttt ttggttgata
    51061 ataacaaagt gctggggtaa aatcactagc ccaaggaata atcgtttcat tactcgtttg
    51121 ttgacgcaaa atattcaatt tttgatcata aaactgatcg ccatatttgt cataaaatcc
    51181 atattcatca gcactataaa aagcatcttg aaatttttcc agcacacctg ctgctgaagt
    51241 gtctgccatg gagataatat ccggcatatc ttcgtgtgtt accaattttg gggtgatcat
    51301 ttgtactgtt gctgttcccg aagctacatt cacaattttt aagtcaatat ttgctccagt
    51361 tttgcggtta tagatttttg cagcattttc aacatagttt gtcagtttgc cgtttgcgac
    51421 atatacttct aatgtagtgg ctttttttat cattggatct gcgatgtttg ctgttacttc
    51481 ttcgttagtt tttccacatg cacttaagct gaataatagg gtaatgttta gcaaagctat
    51541 caccaatttg tatctttttt tcatgtctat cccttctttc ttttcaagtt cttatataaa
    51601 acgctttcac tta
//
LOCUS       DACDIH010000013        51214 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDIH010000013 DACDIH010000000
VERSION     DACDIH010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.013"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            290..1135
                     /locus_tag="IV281_000783"
     CDS             290..1135
                     /locus_tag="IV281_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8779817.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="IV281_000784"
     CDS             1416..2636
                     /locus_tag="IV281_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP8779818.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="IV281_000785"
     CDS             2633..5353
                     /locus_tag="IV281_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8779819.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="IV281_000786"
     CDS             5337..6281
                     /locus_tag="IV281_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8779820.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6350..7360)
                     /locus_tag="IV281_000787"
     CDS             complement(6350..7360)
                     /locus_tag="IV281_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8779821.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7540..7884)
                     /locus_tag="IV281_000788"
     CDS             complement(7540..7884)
                     /locus_tag="IV281_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8779822.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7877..8311)
                     /locus_tag="IV281_000789"
     CDS             complement(7877..8311)
                     /locus_tag="IV281_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP8779823.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8449..9186
                     /locus_tag="IV281_000790"
     CDS             8449..9186
                     /locus_tag="IV281_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8779824.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9179..10390
                     /locus_tag="IV281_000791"
     CDS             9179..10390
                     /locus_tag="IV281_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8779825.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10597..11376
                     /locus_tag="IV281_000792"
     CDS             10597..11376
                     /locus_tag="IV281_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP8779826.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11446..12108
                     /gene="trmB"
                     /locus_tag="IV281_000793"
     CDS             11446..12108
                     /gene="trmB"
                     /locus_tag="IV281_000793"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP8779827.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12206..13960)
                     /gene="ade"
                     /locus_tag="IV281_000794"
     CDS             complement(12206..13960)
                     /gene="ade"
                     /locus_tag="IV281_000794"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP8779828.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14092..15021
                     /gene="cpaF"
                     /locus_tag="IV281_000795"
     CDS             14092..15021
                     /gene="cpaF"
                     /locus_tag="IV281_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP8779829.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14978..16027
                     /locus_tag="IV281_000796"
     CDS             14978..16027
                     /locus_tag="IV281_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP8779830.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16024..16302
                     /locus_tag="IV281_000797"
     CDS             16024..16302
                     /locus_tag="IV281_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP8779831.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16277..16747
                     /locus_tag="IV281_000798"
     CDS             16277..16747
                     /locus_tag="IV281_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP8779832.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16764..17066
                     /locus_tag="IV281_000799"
     CDS             16764..17066
                     /locus_tag="IV281_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779833.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17083..17454
                     /locus_tag="IV281_000800"
     CDS             17083..17454
                     /locus_tag="IV281_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP8779834.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17426..17785
                     /locus_tag="IV281_000801"
     CDS             17426..17785
                     /locus_tag="IV281_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779835.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17957..18964
                     /locus_tag="IV281_000802"
     CDS             17957..18964
                     /locus_tag="IV281_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8779836.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18989..20173
                     /locus_tag="IV281_000803"
     CDS             18989..20173
                     /locus_tag="IV281_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP8779837.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20610..22193)
                     /locus_tag="IV281_000804"
     CDS             complement(20610..22193)
                     /locus_tag="IV281_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8779838.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22771..22914)
                     /locus_tag="IV281_000805"
     CDS             complement(22771..22914)
                     /locus_tag="IV281_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP8779839.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23137..23616)
                     /locus_tag="IV281_000806"
     CDS             complement(23137..23616)
                     /locus_tag="IV281_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8779840.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23689..23973)
                     /locus_tag="IV281_000807"
     CDS             complement(23689..23973)
                     /locus_tag="IV281_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779841.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24051..24245)
                     /gene="ssrS"
                     /locus_tag="IV281_000808"
     ncRNA           complement(24051..24245)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IV281_000808"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24317..25603)
                     /locus_tag="IV281_000809"
     CDS             complement(24317..25603)
                     /locus_tag="IV281_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP8779842.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25741..26412
                     /locus_tag="IV281_000810"
     CDS             25741..26412
                     /locus_tag="IV281_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP8779843.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26378..26866
                     /locus_tag="IV281_000811"
     CDS             26378..26866
                     /locus_tag="IV281_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP8779844.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26879..27826
                     /gene="prmA"
                     /locus_tag="IV281_000812"
     CDS             26879..27826
                     /gene="prmA"
                     /locus_tag="IV281_000812"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP8779845.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27827..28591
                     /locus_tag="IV281_000813"
     CDS             27827..28591
                     /locus_tag="IV281_000813"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP8779846.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28824..31037
                     /locus_tag="IV281_000814"
     CDS             28824..31037
                     /locus_tag="IV281_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP8779847.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31048..31494
                     /locus_tag="IV281_000815"
     CDS             31048..31494
                     /locus_tag="IV281_000815"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP8779848.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31665..32924
                     /locus_tag="IV281_000816"
     CDS             31665..32924
                     /locus_tag="IV281_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP8779849.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33013..33960)
                     /locus_tag="IV281_000817"
     CDS             complement(33013..33960)
                     /locus_tag="IV281_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8779850.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34354..35646
                     /locus_tag="IV281_000818"
     CDS             34354..35646
                     /locus_tag="IV281_000818"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP8779851.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35661..37430
                     /gene="aspS"
                     /locus_tag="IV281_000819"
     CDS             35661..37430
                     /gene="aspS"
                     /locus_tag="IV281_000819"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP8779852.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37539..38414
                     /locus_tag="IV281_000820"
     CDS             37539..38414
                     /locus_tag="IV281_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8779853.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38680..40563
                     /locus_tag="IV281_000821"
     CDS             38680..40563
                     /locus_tag="IV281_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8779854.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40638..40922)
                     /locus_tag="IV281_000822"
     CDS             complement(40638..40922)
                     /locus_tag="IV281_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8779855.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40912..41646)
                     /locus_tag="IV281_000823"
     CDS             complement(40912..41646)
                     /locus_tag="IV281_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP8779856.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41775..42398
                     /locus_tag="IV281_000824"
     CDS             41775..42398
                     /locus_tag="IV281_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP8779857.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42590..43711
                     /locus_tag="IV281_000825"
     CDS             42590..43711
                     /locus_tag="IV281_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP8779858.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43718..46846
                     /locus_tag="IV281_000826"
     CDS             43718..46846
                     /locus_tag="IV281_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP8779859.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46865..50059
                     /locus_tag="IV281_000827"
     CDS             46865..50059
                     /locus_tag="IV281_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8779860.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50205..50912)
                     /locus_tag="IV281_000828"
     CDS             complement(50205..50912)
                     /locus_tag="IV281_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8779861.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16760 a         8950 c        10632 g        14872 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg
     6361 aatcagttgt ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac
     6421 tctttgttga agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg
     6481 cactcaacac attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt
     6541 caccgattac tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt
     6601 ttagttgatc tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga
     6661 caacgtagta ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct
     6721 caccgtcttt caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg
     6781 agtcgaattt aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg
     6841 ctaatttaga gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt
     6901 cacttgataa tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt
     6961 tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt
     7021 tacgaatata ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa
     7081 aagtatcacc taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta
     7141 ctttatcgcc atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt
     7201 ggttcgatga ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt
     7261 tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca
     7321 acgcactagt agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa
     7381 ttagttatta tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg
     7441 tggtagtttc aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc
     7501 tcacttatat agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc
     7561 tggcagatca gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc
     7621 ttggaattta aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga
     7681 ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa
     7741 atcatttgtc agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt
     7801 cccgctcctg ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag
     7861 tcctttgaaa aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag
     7921 cttttagttc ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat
     7981 cctctttgct gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta
     8041 gttcacggtt gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg
     8101 cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa
     8161 aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga
     8221 tgtctaaaaa agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat
     8281 tgatgatttt gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat
     8341 acttcagctt aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc
     8401 attttcttcg tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt
     8461 atcgatcatg tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat
     8521 gtacagcctg gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc
     8581 atcaagcaca ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta
     8641 tccttaaaga aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct
     8701 tctttatatg aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat
     8761 atacctatgg atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct
     8821 aatcgtcttg actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc
     8881 ttatgcgcct ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg
     8941 gatccactag ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc
     9001 ggtattttga tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg
     9061 gtcttacata acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt
     9121 cagctgccgg attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat
     9181 gagtagtttt tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg
     9241 ttatgtattg aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt
     9301 atattattct aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat
     9361 ctctgtattt tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc
     9421 agatattgtt tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt
     9481 tcgctattct tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc
     9541 attagtggtg gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt
     9601 atggagtttg aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca
     9661 acaagttatt cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct
     9721 atcattttat tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata
     9781 ttactggttt ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt
     9841 agaagaacat aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga
     9901 aattacggca aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag
     9961 agaatcaaaa aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt
    10021 tagtggtctt tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga
    10081 cttttatttt agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt
    10141 acctttagcg aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa
    10201 tcagaagaag caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat
    10261 ttttggtttg attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc
    10321 ttatttgatt gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat
    10381 gtcagactga aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca
    10441 gtaagaatgc tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa
    10501 atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac
    10561 tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag
    10621 tggcggttac gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag
    10681 gacaaagtct ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga
    10741 attacaccaa aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa
    10801 gagtggctgg aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt
    10861 gatgttttgt accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt
    10921 ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa
    10981 ctccagaaaa atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca
    11041 atagataagg aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta
    11101 tgtaaaaatt atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg
    11161 gatattgcaa cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc
    11221 agttatggca tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg
    11281 aatttgctcc aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa
    11341 gaaattttac aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga
    11401 tttggccaga cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat
    11461 cgtccgggag cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca
    11521 ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt
    11581 ggtatgggga aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat
    11641 attggagtag aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa
    11701 ccgcttccta acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct
    11761 gacagcgaag tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat
    11821 gaaaaacggc gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca
    11881 aatggagaaa tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt
    11941 ttttctcaat atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt
    12001 gaaggcaata ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat
    12061 cgagtagaag ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct
    12121 gctttcataa aaacattaat atccataaat aaatcccaga aaatgaacat tacagatgta
    12181 atgtattttc tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag
    12241 gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg
    12301 gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc
    12361 ctagttgatg tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt
    12421 cctgtgtcat gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga
    12481 caacggtcat cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt
    12541 cgtttgtccc aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc
    12601 cttgtttcag ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac
    12661 gctcgatcac cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt
    12721 gtcggtccac tttttctacc aaatctttag tcactagact attcgggata atttcgatca
    12781 catgggcttg tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt
    12841 ttagttttgg cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga
    12901 gttgcccaga ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg
    12961 taagatcgtc tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt
    13021 tcaaatcaaa acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga
    13081 ttccaagtgc aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat
    13141 tgatcagttt atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg
    13201 gtagtagcga tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa
    13261 gatccaaacg ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg
    13321 ctgcaggata aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt
    13381 tcttatgctg gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg
    13441 cttgatagtt catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta
    13501 atttttttgc atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag
    13561 gtagcataaa aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac
    13621 ctgtactccc agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat
    13681 gaagcaagga tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat
    13741 ggccatcgat gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt
    13801 cgtattcacc gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt
    13861 ctttcgtaaa gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt
    13921 tacttgctac ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa
    13981 atttttataa gtataacata aattcggttt ttgccaatat ttttttgtaa attcatttta
    14041 tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata
    14101 aaacgattat cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat
    14161 atattgccag cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc
    14221 tggaagaaag tctcttcggc agatggacag caactgatct caagatttaa gtatttggga
    14281 cagatggata tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa
    14341 aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta
    14401 gtgattcggc tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat
    14461 tttgcaacta ttttacagaa aaccgtcaga cgtggattat accttttcag cggaccagtc
    14521 ggatcaggca agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt
    14581 actatcgaag atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg
    14641 aaaatagcac agacttatga tacacttttg agactcgcat tacggcatcg tccagacttg
    14701 ctaatcattg gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta
    14761 acagggcacc gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga
    14821 atccatgaac tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat
    14881 caagaacttt tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg
    14941 gaggaagaat cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt
    15001 tggcagtcag aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac
    15061 catgggagaa ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac
    15121 gatccgttcg tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca
    15181 agcgtttagt gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga
    15241 gttagctgaa cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg
    15301 gcttgttgaa ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct
    15361 cacctttttg ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg
    15421 ttcaggaatg gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta
    15481 tgttctacta ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat
    15541 gaagttgtct cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat
    15601 atattccttg tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg
    15661 tttcgaatta aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca
    15721 gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt
    15781 ccagaattat cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa
    15841 gggaagttta ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa
    15901 acgtcttcat cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt
    15961 gattttatta gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt
    16021 gccatgaaaa agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc
    16081 gtcttactga tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg
    16141 aatcatgtag accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag
    16201 ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc
    16261 tatatcaaac gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc
    16321 aggatatacg ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt
    16381 tccagttctc gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca
    16441 atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg
    16501 cttttattgg aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat
    16561 aaattgggaa gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac
    16621 ttttgctgct agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga
    16681 aaaagagcgg acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat
    16741 tgaataaaac aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct
    16801 gtgtctactt agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg
    16861 aggaactgct atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca
    16921 aaagaccaga gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg
    16981 gagtaatcga aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa
    17041 gtaattccag catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta
    17101 ttgctcacat tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt
    17161 aaagatcaaa aagagtggct aatattttta gcacaattag aagaagaact caagagcagt
    17221 cacaatgtct ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc
    17281 atcgaaagat atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg
    17341 ttaacaagta tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta
    17401 cactttgaaa atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg
    17461 ttataagggc tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt
    17521 gatggcagca ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta
    17581 ttatatagca aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag
    17641 ttcggaagga gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc
    17701 cctattctta gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa
    17761 tgatacagat actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat
    17821 aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat
    17881 tgaaattttg tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct
    17941 agaaatgagg tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga
    18001 agctattcag cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa
    18061 tgcagaaaat ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga
    18121 gacaacgcaa cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga
    18181 agaatggcgc agactgtctc aattactttt attgaagggc agccagacag aacatttgca
    18241 ggcgaaccat caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca
    18301 actatacaca aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa
    18361 cttgttatta acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg
    18421 tgtggatatt gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc
    18481 aaatgttcaa tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt
    18541 tgcaatcagc gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt
    18601 aacaagtacc gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa
    18661 atatgtgaag ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga
    18721 tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca
    18781 attgccagat gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa
    18841 aaaaggacca caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa
    18901 agaaccagag aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct
    18961 gtaatcattc aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag
    19021 ttcaagtttg aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat
    19081 cgtcgaacgt atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa
    19141 attcgaacaa atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca
    19201 gttgattgaa ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt
    19261 tgttcacgga ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg
    19321 cattgctgaa ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa
    19381 agcgtttaaa aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca
    19441 ctcaacaatg ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt
    19501 caaagtaaga aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc
    19561 aaaattattg aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg
    19621 tgtatcgatc acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc
    19681 attagctggg gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata
    19741 tttaatggaa aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc
    19801 tggtctgcta ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga
    19861 aaaagaacat attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg
    19921 cggttatgtg acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga
    19981 gaatgacgga catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat
    20041 cgatccagcg ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa
    20101 agtaagagta ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg
    20161 tctgcgcggc taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa
    20221 ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc
    20281 cttctttttt acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa
    20341 gactggcatt ttatttattg attttcagga aaatcccaat attttatttt cattcatctt
    20401 tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt
    20461 cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga
    20521 ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa
    20581 aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg
    20641 ctgatctgct cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg
    20701 tcatggctga ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct
    20761 cgagcatagc tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct
    20821 aaaaagagtt tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt
    20881 ttacccacat cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg
    20941 tttccagtca atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc
    21001 tgctgcggca aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc
    21061 ttttcctcac cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg
    21121 atggctaaat gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca
    21181 gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta
    21241 gacaagcttt gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc
    21301 aggcggcgtt ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc
    21361 cactgacgaa aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg
    21421 taattttttg tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt
    21481 ccgggatact caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg
    21541 agaaattgcc gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt
    21601 tccaaccagc gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa
    21661 tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt
    21721 tctgttaacc gtgcttcctg acggtgtgga atccctaagc cttgcaagat cgctgcaatc
    21781 cggtcttcta atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg
    21841 gccagacact tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt
    21901 tgctgtttga ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct
    21961 tcggaaactg taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata
    22021 acaccgctgt cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt
    22081 ccgttgatac cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca
    22141 aataatggat tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct
    22201 ctccttgaga ccactcaaaa aggccatggt tatccatggc ctgtgttatt taaactcaga
    22261 ttatcatgag aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg
    22321 acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt
    22381 atcgttggta attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc
    22441 ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac
    22501 atttacttaa tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg
    22561 gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca
    22621 tatggtgttt gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg
    22681 ttgcattaaa cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt
    22741 gtggttgcaa tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc
    22801 acttgccttc tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt
    22861 atccgccatc gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac
    22921 gaaccaataa gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc
    22981 ctggtgttca ctttttttta ttttacttga actcattgac tgttttccct ttattttaaa
    23041 cattataaag accgatgagt gcttgccgcg tcttttgttt ttatcacggt tttttgtgca
    23101 aaaaaacctg ccgatagttg gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc
    23161 tggcaattgg ttttctaaat cagttcggac aacaagcaca tcgcaagctg cgtttctaat
    23221 aacatattca gaaacagaac caataaataa acgttctaca gcattcaatc ctgtggctcc
    23281 taacataatc aaatctactt gattatcttc tgggatttct ctggcaataa tttgttttgg
    23341 tgagccatat tctacgacag aggttacatt attcaagcca gctttcttcg cattgctttc
    23401 ataatctgcc aatgtttgct ttgccatttc agtagcttgt tctgcaagca ttccatcaaa
    23461 ggatgaaacg gtttggaaag aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt
    23521 atttcggata gctacattga ccgccttttt aaaagctaat tcagcttcat cagaaccatc
    23581 tacggcaacc atgatttttt tgtattgttg taacataagc attcactcct caagtcaatc
    23641 gtattgtact cttttattgt agaacacttt agggaaaaag agaattattt actgctttgg
    23701 ttcagcaatt ttttttgcaa atcggatact gaaggtggca gaaacaataa ttacgatcag
    23761 taaaaagacc aaggcaataa agcgttgatt gaaaaaagct gttaaaatac ccattacgcc
    23821 taaaacagca tatgcagctc cgtaaaattg taggaatcct tgatttttat catttttgtt
    23881 gatcaataca aaaaagatat cctgtttatt aaataaataa tagctaacga ataataatac
    23941 taaagtggcg ataatcatta aaatcctaat catgaacaaa tcctccaaaa taagcaacaa
    24001 atagaaaaag caatttgcca agagcaaatt gctttattct ttaaaagatg gtaatctccg
    24061 ttaatgccgt tgtttgtcct gtagtattga tcccgcgaat tcaagcaggt gggtcccatg
    24121 gtcgtagctt cgaactacca aatgataagg catgttacca gcagtgacac aagacgggtc
    24181 ccaagatatc aataaaaatg ttcggtcaac acacaaagag gacaattcga acatcacaga
    24241 tttcccacct ctatagtagc atatgaattt aaaaagagca atgagagagc tcgcagaaac
    24301 agaaccgttt tcttgtctag ttttttggag gatgcttttt ccactctttt atacggtaat
    24361 actgttgacc cagagcctgt tcatatttcc ctgtatcttt tggctgataa tactgagcat
    24421 tcttcagttt atctggtaaa tattgctgat tgacccaagc ctgatcaaaa tgatgaggat
    24481 actgataacc cacgccacgg ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat
    24541 gatcaggaac atctccagct ttgccctctc gaatatcagc aagtgctgca tctaatgcca
    24601 tgtaggcaga attggatttt ggcgacaagc aaagatcgac gacgacgtct gctagtggga
    24661 tacgtgcctc aggcaatccc aatttttcag cagctaatac agcgttcact gtacgagccg
    24721 cagcagcagg attacctaaa cctatatctt cataaccgat aaccatcaga cgccggcaaa
    24781 tacttgccag atcacctgcc tcaactaaac gggcgagata atgaagtgca gcatccacat
    24841 cacttccacg aatcgatttt tgaaaagccg aaataacgtc ataatgagca tctccatttt
    24901 tgtcatgggt aagagctttt ctttggacac attcttcaat gatcgacagt gtaagatgaa
    24961 tccttccttc tttatctgaa ggagtcgata gtgtagccag ttccagccca ttcaatgcac
    25021 tccgcaaatc gccgttcgtt gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga
    25081 ttgcttgttg tccaaggccg cgttctttat cccttaaagc atgttcgact gccaactgga
    25141 tgtcttgttc tgttaatggt ttgacttcaa agatctgtgt acggctgcgg atcgctggat
    25201 tgatggtaat ataaggattc tcagtagtcg ctccgatcaa gatgattcgg ccactttcta
    25261 aatgaggaag cagaaaatcc tgttttgttt tatctaatcg gtgtacttca tccaataaga
    25321 ggatcactgt accactcatt tttgcttctt ccgctacaac ttgtagatcc ttttttgaat
    25381 ctgtagcggc gtttagcatg cgaaatgcat aattagtaga gccggcgatc gcacttgcga
    25441 tactcgtttt tccagtacca ggaggtccat atagaatcat ggaagagagc atgcgagcat
    25501 cgaccatgcg gcgtatgatt tttccttccc cgactagatg ctgctgacca acgacttcat
    25561 cgatcgttcg aggtctcatg cgataagcca atggctgttg cataggattt tcctccttct
    25621 tgtttttacg tcccttaatt atatcatatt tagcaagatg cctagaaaga acagccttct
    25681 gttgttttgt tttcatagct tatccaacta atattgtgta tgataaagta ggtgagaaaa
    25741 atgacggata aacaaaagat agatgaattt ttttggaata aaacgacgcc agaagtggca
    25801 cgggatttat tggggatgta tttggagcat gacacaccag aaggaagatt agctggatat
    25861 attgtagacg cggaagcata cctaggtcca gaagacgagg ccgcccatag ctatggttta
    25921 agaagaacac cgcgtgtgcg ggcaatgtat gaaaaaccag gtacgatcta cctttacacg
    25981 atgcatactc atcggatttt gaacatcatt actcagccag aagggattcc tcaaggagta
    26041 atgatacgtg caatcgaacc cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga
    26101 aaaactggtc cagatatcag caacggtccg ggaaaattag tggaagcatt agcaattcct
    26161 caagaacttt atggtcagtc tatagcagat agttcgttac gcttagtttt tgagaaaaaa
    26221 aagacaccaa aaaagatcaa tgctttgcct agaatcggta tcccaaacaa aggtgtatgg
    26281 acagaaaagc cgttgcgttt tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa
    26341 aaccaaatcg agaaagattg gggttggagg aaagaaaatg aaaaagaagg atctattaac
    26401 atatttagat gaaataattg aaaaacaagt gacagactat gatgtagctt tagattggga
    26461 tacaaaaaat catacgatcg agatcgttgt tcgcttgttt gcagaaaata aagcacaatt
    26521 tgagattgat gatgcagaag ggatcgtttc gcaagaacca attatcgaat ttgaagacgg
    26581 cgtacttctt tttaatcctc agaaatctgt ttttgatgaa cgcaattatc tagcagtaat
    26641 cccttatgaa ggaaaaaaag ggttggcaaa atcagtagct gattctttag tggaatatct
    26701 aaatgaagta ttagctcaag ggcaaagcga tttgcttgat tttttggatg aagacgatga
    26761 cgaagctgta ttcgagcttc actgggataa tcagcaacta gcagaattag tagaagtaaa
    26821 acagcaaaat gaagcagata cttatttacc gtacccaagt tattaaggag tgacagatat
    26881 gaaatggaat gaagtaaaag tagaaactga aagtgaagca gtcgaagcag ttgctaatat
    26941 tttgatggag gcaggggcga gtggtgtcgc gattgaagat tctttagatg ttgaacattt
    27001 tcagtcggat caatatggtg agattttaga taaaaatgat tttacatcgc tgaaagaagg
    27061 cgcactagtc atggcttatt ttccggaaac gactttctta ccggaaatct tgccttttat
    27121 caaagatcgt atcacacaat tacctgagtt tggtctagct attggaaaaa ataaagtaac
    27181 agttagtgaa gtagaggaaa gcaactgggc aacagcctgg aagaaatact atcatccggt
    27241 gcgtattacg cgaatgctga cggttgcgcc aagctgggaa aactaccaga cagaagatcc
    27301 tctagaaaaa attatcacac tagatcctgg tatggcattt ggcacaggca ctcatcccac
    27361 gacttccttg acattgacag cactagaaac agtcttgcgt ggtggtgaaa cattgctaga
    27421 tgtgggaact gggtcgggcg tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt
    27481 ctatgccttt gatttagacg aagtagccgt tcgttcggcg aaagaaaata tggatatgaa
    27541 tgaaatagcc aaagatgtcc atgtatcagc taacgatctt ttgacaggaa tcgagattga
    27601 aagtgatgta atcgttgcga acattttagc agatatcatc ttattgatga tcccagatgc
    27661 ttggcgttta ctgaagcaaa caggcacgct gatcgtttca ggaatcattg aagagaagaa
    27721 gcaaatggtt ctggatgcca tgttagaaca agggtttatc gtggatcaaa ttttccagca
    27781 aaaagactgg tatgcaatta ttttgaaaaa accagaggtg gaatagatgc agcggtattt
    27841 tttaaaagaa tcatatgaat caaaagacta ttttgtttta gaaggggaaa actatcacca
    27901 cattgtacgg gttatgagga tggaacccga ggatgaagtt tttttggcat tcaatgatca
    27961 aatagccatt gttgcagaaa tcacggagat tactgaagag gctgtttatc tgaaagaaat
    28021 taaaaaagaa gaaaatcaaa aggaactgcc aagttcgatt accatagcga gtggctatcc
    28081 taaaggggaa aagctggatt ggatcgttca gaaatccact gaattaggag cgcaagcttt
    28141 tcttggtttc ccggcatcag cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg
    28201 acaacaacgg cttgaaaaga ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt
    28261 gccatcagta gagttattgg aaacgaaaca agatttattt gagcggctag atgattatga
    28321 cgtggtcttg gttgcttatg aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa
    28381 aattcttagt gaaacaaaac cagatagtcg aatcttagct gtctttggac cagaaggtgg
    28441 attatcccgc gatgaagtcg attattttgt agaacacaaa gggatacttt gtggattagg
    28501 accaagaatc cttcgtacag aaacagcacc tctttattta ttaagtgctg ctagctatca
    28561 atgggaatta atgaatcctt ctttcagcta acgttcttca ttaataaaag agtatcgaca
    28621 gaaagaattg aaaaaatagt caacaatttg tataatagaa acaattattt atacaatgga
    28681 tacgaaaaat cgtttgaaaa agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa
    28741 ctgcacggat tttgggacaa ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa
    28801 aaaagaaagg gggatcgatt cctgtgacaa aagaagaaat tttaacggga cccggtgtca
    28861 taaagctcgt tagtcaatac atgggtcccg aacatgtagc atttgttgag aaagcatgcg
    28921 aatacgcgac tgctgcacat gacggacagt ttagaaaatc aggcgaaccc tacattattc
    28981 atcctatcca agtcgcaggt atattagcag atttaaaaat ggatccccat acagtggcta
    29041 caggcttctt acatgatgtt gttgaagata cagaaatcac tttagaagat ctgagagaag
    29101 aatttggcga tgacgttgct atgttagtgg acggcgtaac caaattaggg aaaataaaat
    29161 ataaatccca cgaagagcag ctagcagaaa accaccgaaa gatgctgcta gcaatggctc
    29221 aagatttacg agttatcatg gtcaaattag ctgatagact acataacatg cgtacgttga
    29281 agcacctgcg agaagataag caaagaagga tcgctcagga aactttagaa atctatgcgc
    29341 ctcttgcaca tcgtctaggg atcagccgga tcaaatggga attagaagat acagcacttc
    29401 gttatctaaa tccaaaacag tattaccgta tcgtccactt gatgcagacg aagagagaag
    29461 aacgtgaaaa atacgtaagt ggtactgttg aagatattcg aatagcgaca gaagagctgg
    29521 ggatttttgc agaaatctat ggacggccaa aacacattta ttcaatctat cgtaaaatga
    29581 aagatcagaa aaagcagttc aacgaaattt atgacttgct agctattcga gtgatcgtag
    29641 attcaatcaa ggattgttat gctgtattag gagcaatcca cacaaaatgg aaaccaatgc
    29701 ccggcagatt taaggattat attgccatgc caaaagctaa tatgtaccaa tctttgcata
    29761 ctaccgtgat cggtccggca ggaaatccgg tagaaattca aatccgaaca caggaaatgc
    29821 atgaaatcgc tgaattcggg gttgctgcac actgggccta taaagaagga aaaaatgaaa
    29881 aagtagaacc agatggtatg acgaaacaat taagctggtt ccatgagata ctcgaacttc
    29941 aagacgaaag ctatgatgct tctgaattta tggaaggcgt aaaaggagat atctttagtg
    30001 ataaagtcta cgtcttcaca ccaaaaggag acgttactga gttaccaaaa ggatccggac
    30061 cattggactt tgcatacagt atccatacag atatcggtaa caaaaccact ggtgcaaaag
    30121 taaatggcaa aatggtgcag cttgattaca aattgaaaaa cggagatatc attgagatca
    30181 tgacttctcc aaattcattt ggcccaagtc gcgactggtt gaaattagtt gctactagca
    30241 aagcaagaaa taagatcaaa cgtttcttca aagcccaaga tcgagaagaa aatgtgatca
    30301 aaggccacga atccgtggtc aaatgtatta cagatctagg atttacgcct aaagatattt
    30361 tgacgaagaa caaactgcaa gaagcactcg atcgttttaa ttatcaaaca gaagatgatc
    30421 tctatgcagc tgtagggtat ggagaagtta gccccttgac gatggccaat cgtctgactg
    30481 aaaaagaacg taaagaacaa aaaatcgagc agcaaaagca agaagcagaa gaaatcatga
    30541 atcagccgaa aaaagaacct gacaaaatga aagtacgtca tgaaggtggc gttgtcattc
    30601 aaggggtaga aaacttacta attcgtatca gtcgctgctg taatccgatt cctggtgatg
    30661 atatcgttgg ttatatcact aaaggcagag ggatatccat tcatcgtcga gattgtccga
    30721 atgttcagcc tgacaaacca aatgtagcag aacgtttgat tgaagtcgaa tgggaagata
    30781 catcgaatac acgaaaagag tatgatgcag atttggaaat ttacggctat aatcgttcag
    30841 gcttattgaa tgatgtactt caaacagtca atgcgctaac gaaaaatctc aacagcgttg
    30901 aagcacggac gaataaagat aaaatggcga cgatccattt gacggttggt atccagaatt
    30961 tatcccacct aaagagtatc gtggataaaa tcaaagcagt acctgatgtc tacagtgtac
    31021 gccggacgaa tggataggag gacagatatg agagcagttg ttcaacgggt aagtaaagct
    31081 gctgtgtcag tcgatcagca agaaattgga aaaattggct acggcctact tgttttatta
    31141 ggcattcatg aaaccgatac gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa
    31201 atgcgtattt ttgaagatga acaaggaaaa atgaatatta gtgtagaaga tgtgggagga
    31261 caaattttaa gtatctctca attcacactg tttgcggata cgaaaaaagg aaatcgacca
    31321 agctttgtgg aagccgcacg accagaaaca gctattcctt tatacgaatc attcaatgaa
    31381 ggaatcagaa gtcgcggcat cacagtcgct accggacaat tcggagcaga tatgtcggtt
    31441 tctctcgtca atgatggacc tgtaacgatc attattgatt ctaaaaacaa ataaagtaat
    31501 ctggttttca ctagtaaaaa ctatgaaaca cgtcgcttaa gttagactga taaactctaa
    31561 cttaagtggc ttttttgtct gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt
    31621 aagcttaaat tatacaagtt attttttata ggaggttgct gaagatgaaa gaagtaccag
    31681 tagtagatat caaaatcaca gatttaaaaa aattgtatca aaaaggatac aacatgttgg
    31741 aggagttacg ccatgaagcg gacgctcccg tcgtcaaagc aaagattttc aataaagaag
    31801 ctatcacgat ctacggatca tcagcagcaa aagtatttta tgatccaagg aatttcaaac
    31861 gaaaaggtgc tatgccaaaa ttagtattga agacactttt tggtcaaggt ggagtacaga
    31921 ctttggatgg agctgctcat caccatcgga aaaatatttt tatggatcta atgacaccgg
    31981 aaagaatgga agattatcat cgtatcttag ataagaatct gacacaagca cttgaagcac
    32041 agcatggtca atttgaacta tttgatttaa gtaagatggt tttctttaca tctatttgcg
    32101 agtgggcagg tatcaattta tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat
    32161 atcaaatctc tatgatcagc ggaacgttta cttctccgat tgatcacata aaaggtgtcg
    32221 aaaatcgcaa aaaatcagaa aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc
    32281 ctgtggctgg aaaagaaaat gtggcattat atgcttttgc caatgccaca gatttagatg
    32341 gtcagctttt acctctggaa gtggcagctg ttgaattatt gaacatcatc cgtccaacgg
    32401 ttgctctaac tgtgtgggcg gcattgatgg gacatgcact tttcagtaga ccagatttgt
    32461 atcagcaatt aaaaaatgac ttttcgacgt tacaagatcc gtttattcaa gaaatgagac
    32521 gctattatcc tttcttccca atgctacctg cgatttcgct gaaagaagta gaagtagatg
    32581 gttatcgtat accagaaggc agttgggtta ttttagattt atatggaaca gaccatgatg
    32641 aacgaacagt tgaagcgcca gattccttta tgatcaaacg gtatgtaggg aaagcaaaag
    32701 atatttccta taaagaagaa tatgagatga ttgcacaagg cgggggaaat tttcgacaaa
    32761 tgcatcgctg tgcaggagaa tggatcactc ttcatagtct acgtgtgttt tccgatcagc
    32821 tagtaaataa gtttgagttc tctgtacctg aacaagattg gacgattcca ttcaatcagt
    32881 ttccgactta tccgaatagc cgagcgctac tttacaagaa ttagattagc ataaacttat
    32941 cgaaaaaaag gaagatcaaa cagtgctgat cttccttttt tcatattact ataaattgat
    33001 aaggtcaatt gatcaaaatc cacgaaatgc ttcatcaatg gtttgataat cttcattgga
    33061 taaatgcaaa tctaaagctt ggacattgtc agatacttgc tctggacgct tagctccagg
    33121 tataactaca gacactcgtg gattcttcat gtaccaagct aaaacaagtt gcgcaggagt
    33181 ggcttggtat cttttagcaa tggggcgaag tacatcgaca gcggttaaaa tttctctgaa
    33241 acgttctcct tggaaatctg gatttctttt tcgaggatct ccttcaccaa attgtttttc
    33301 ctcaccaagt tcatattttc ctgtcagcaa accggaagca agaggaaaat agggaacaaa
    33361 actgattttg tttttttcaa gataagggaa taattctttt tcagcctgcc gatggatcaa
    33421 actgtattta tcttctacta catcgacata tccatctgca ttcgcttctt tcaattgttc
    33481 taacgtgaag tttgacacac cgactgctcg gatttttcct gcttctttta attcgtgcaa
    33541 tgtagcaact gattcatttt ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc
    33601 gatgtaatca gtttgcagac gttttagtgc atcttccacc gattgtttga gaaactcagg
    33661 agaattatca aatgttcgcc ctttatttgg tacatgagca gcttttgtag cgatgacgac
    33721 acgtgagcga tcatattctt tttccttcag tacctcgcca atcagttctt cggaacggcc
    33781 gtttccgtaa gcataagcgg tatcgatcaa attgatcccg ctattcaaag ctgtacgaac
    33841 tacttgtttt cctgtctcat cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt
    33901 tgctcctaat ccaagctgtt ctgcgtagac ttgtgaatgt ccgattctta tttctgccat
    33961 agctgattcc tcctttttgt ctagtctaaa acaaaatacc aaaaagctga actaaaacgt
    34021 ttttgtgagg ggaaatagtc agatatctct tgactttgag actggttttt agtaggttat
    34081 aagtaatatc gaatgtcaat gaaagagcga gtaagttttt gaaactttac aagagaggat
    34141 catcgaggct gaaaatgatc taaagtttca ttagcctaaa gacactctgg agatggacga
    34201 gtgaaaagcc gtcccgcgta cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat
    34261 taggtggtac cgtgtatgaa tttcattcac ccttgttcat gatgaacaag ggtgattttt
    34321 ttatttacat agctaagaaa gggagagaaa actatgagtt tccaaagacc aaaaggaaca
    34381 aatgatattt tgccaggcga atcagaaaaa tggcagtttg tcgaagaaac agcacgttta
    34441 ttattcagag attatcagta taatgaaatg cgtacaccga tttttgaaca ctatgaagtg
    34501 atatcccgaa gtgtagggga tacaacggat atcgtttcaa aagagatgta tgatttttat
    34561 gacaaagggg aacgccatgt gacgcttcga ccagaaggta cagcaccaat cgttcgttct
    34621 tttgttgagc ataaattgtt cggcccagag tatgcgaaac cgttcaaaac tttttatatg
    34681 ggaccaatgt ttcgctacga acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc
    34741 ggagtagaag catttggatc agaaaaccct gcaactgatg tggagagcat ggtgatggca
    34801 cttgatttct tcaaacagtt ggggatcacg caaatccggc tagtcatcaa ttcattaggt
    34861 gataaagaaa cgcgtcaagc ctatcgtcaa gcattgatcg actatctttt accttttgaa
    34921 tcagagctga gtgaagactc taaacgccgc cttcatgaaa atccattacg cgtattggac
    34981 agtaaagaca aacgagatca aaaatttgtg gcagaagctc catctatctt agattatcta
    35041 agcgagcctg ctcaaaaaca ttttgatacc gttgtttcca tgctggatgc attggctgtt
    35101 ccttatgaaa tcgacagtaa tatggttcgc ggactagatt attacacaca tacgattttt
    35161 gaaatcatga gtgacgcacc aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt
    35221 tatgacaatc tagtggaaga actaggtggt ccttcaacac ctggcttcgg atttgcaatg
    35281 ggtatcgagc gattactctt gactatggag gcagaggaag tagttatccc tgcatttaat
    35341 gagttggatg cttacgtggt cgcactcgga gacgaaacaa atatcgaagc attgaaagtg
    35401 gtccaagcga ttcgaaactt tggtttttca gctgaccgcg acttcatgaa ccgtaaagca
    35461 aaagcgcaat ttaagacagc agataaagcg aatgccaaat tagtattggt gatcggtgaa
    35521 gatgagcttg ctagtcaaac gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt
    35581 tttccattag aagagatata tgaacatttt gatgaagtct atgatgaaat gactttattc
    35641 gagtaggagg aaaaaaataa atggcacaaa gaacgatcta ttgcggatta gtttccgaac
    35701 aacaattaga acaaacagtt acattaaaag gatgggtaca aaaaagacga gaccttgggg
    35761 gagtaatctt tatcgaccta cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg
    35821 aaatcaacaa agaagcatgg gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag
    35881 tgactgggga agttagaaga cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg
    35941 aattcgaagt gattgcaagt gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc
    36001 aaattgaaga tgatcaagca atcagtgacg aaatacgttt gaaatatcgt tatttggatc
    36061 tgcgtcgtcc aaaaatgact aaaaacatca aattaagaaa tgcgacaaca aaagcgattc
    36121 gtcattattt agatgataat gactttctcg atatcgaaac accttaccta gggaaatcta
    36181 caccagaagg cgcaagagac tacctagttc cttcacgtgt ccatgctgga catttctatg
    36241 cgttgccgca atctcctcaa ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt
    36301 attatcaaat cgttcgttgt ttccgcgatg aagatttacg tggagaccgt cagcctgaat
    36361 ttacgcaagt agatattgaa acaacgttct taacagcaga agaaatccaa acttacacag
    36421 agggattgat tgcgaaggta atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc
    36481 ctcgtatgac ctatgatgaa gcaatggctc gctacggcag cgacaaaccg gatacacgtt
    36541 ttgatatgga attgatcgat ctaagcgata cagtcaaaga agtagaattc aaagtcttcc
    36601 aaatggctct tgaaaacggc ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc
    36661 gctactcacg aaaagacatg gatcagttag gtcaatatgt aggacaattc ggagctaagg
    36721 ggcttgcatg gctgaaagta gaagaagacg gattgaaagg tccaattgct aaattcatgg
    36781 gagaagcaac agaagcaatc attaaagcaa cagatgcaaa accaggtgac ttgctgatgt
    36841 ttggcgcaga taaaagtgaa atcgtcgctg ctgcattagg tgctatccgg acacgtttag
    36901 gaaaagaatt aggactaatt gatgaatcga aattcaattt cttatgggta actgattggc
    36961 cacaatttga attttcagaa gaagaaggtc gatatgtctc tgctcaccat ccattcacga
    37021 tgccaaaaga agaagatatc ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag
    37081 cttatgatat cgtcttgaac ggttatgaac taggcggagg ttctatccgt attcatactc
    37141 gtgagcttca agaaaaagta ctagaaactc taggctttac aaaagaacaa atggaagaac
    37201 aatttggttt cttgttaaca gctttagatt acggattccc tccacatggc ggtattgcat
    37261 taggattgga ccgttttgca atgttattag ccggagaaga taatattcgt gaagtcattg
    37321 cctttcctaa aaacggaaaa gcagcagatg tgatgagtga tgcaccaagt atggtttcgc
    37381 ctttacaatt gttcgaattg aatattgatg taacgaatat agaagaatag ccgataaaaa
    37441 ggtaaaaagg atttgtcgaa tacgcttctt ttgacaacct tgatttaatt gaaaaacact
    37501 ttataatgaa gccataacat ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt
    37561 gggcttatca tgattacacg accaagcttt cggtatctat cgtttatgcg ttgatcgcat
    37621 cagtcgcatt gaatttcttt tatcagccag gacatattta ttcaagtggt attactggtc
    37681 tagctcagat tttgacgact ttgtcaaaat cactgatagg attcgaagta ccggtatcaa
    37741 caactttgta tttactgaat atacctttgt ttgtattggc gtggttaaaa atcgggaaga
    37801 agtttacaat tttcactttt atcacagtca ctttgacctc cctttgtatc catattttac
    37861 cggaaacagt cctatctacc gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg
    37921 gactaggtat tgggctagcg ttgagaaacg gcctatcatc gggcggtcta gatatcgtga
    37981 gtatcacgat tcgtaaaaaa acaggaaagt cgattggttc tatttcgatc tacttcaatg
    38041 cattgatcgt ttttgttgcc ggaattctat ttggctggca atatatgttt tacagtacgt
    38101 tgtctatttt cgtcagtgga aaagtaactg atgtcgttta tactaaacaa aagaagatgc
    38161 aggtaatgat cgtaacgaaa catccagaaa aagtagtaaa tgaaatccaa aactgtatgc
    38221 gtagagggat cacgatcatc aatgaagcag aaggtgctta taagcatgat aagcagacag
    38281 tattgatgac gatcgttaca cgatacgaac tgcctctact tagagaagca atgaaagctt
    38341 ctgatccaca agcttttgta agtattgcag aaaacgtcca gatacttggt cgtttctatg
    38401 aggatggcat ttagatcgag tttgacaaaa attcatgaaa tactgctgta gacagacgtt
    38461 cgtttgtctg cagttttttt ctaccatagt agaatcagca aagaaaaact gcttttttat
    38521 ttgaaatttt cttaaagttg aaaaaaagca tgtttatttt tttaaattta tgggatgata
    38581 gaaaaaaata gtagagaaag gacacgattc tacttgctat tttcacttaa tacccgtata
    38641 ttacgaatga aaaaagttta tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta
    38701 aaaaacagcc gctacatcac tgggtttgat gggctccgtt cattagctgt ggtgggtgtt
    38761 atcttctatc accttctgcc aactagtatg aaaggcggct atttgggcgt tccgattttt
    38821 ttcgtcgtat ctggttattt gatcacggat ctgctgcggc aggaatggga acaaaatgga
    38881 aaaatcaata tttggcaatt ttatgtcaga agaatgaaga gactctatcc ggcacttgtt
    38941 tttatgcttg tgacagcttc ggcttatatc actttgtttc agcgagggct gttaaacaat
    39001 cttcgaggag ttgtggtcag cagcttactg tatgtcaaca actggtggca aatcaataac
    39061 ggtttatctt atttcgatcg atttgctaat gagtcaccgt ttacgcatat ctggtcttta
    39121 gcagtagaag gtcaaaacta tttggtttgg ccgatcttgt ttgtattgtt gatggtattt
    39181 gttcgtaaaa gaaaatggat cttttatact gtgttaggta catccttcct ctccgccatc
    39241 ttgatgatga tcttgtatac accaggaggc gatccaaccc gtgtttatta cggtaccgac
    39301 actcgtctgt tttctatatg gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt
    39361 ttaaagaaaa acatcccgaa acaagcgaaa aatattctga atatcgctgg aggaatctcg
    39421 cttttggcac tgattctttg cttcttcttt ttagatgatc attacaattt catctattac
    39481 ggaggaatgc tcctagtgag tctgttatgc gtagtgctag ttgcagtgac agcacacccc
    39541 ggagcaagtt tgaatcgatg gctgacaaat ccagtattta cttggattgg caaacgaagc
    39601 tatggcattt atctgtatca gtttccagtt atgatttttt atgaagcaaa ggtgactgat
    39661 attgccgatc atgttttgtt gcatacttta gtggaaatca cattgattct aatcatcagt
    39721 gaactttctt atcgatatgt ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc
    39781 caagtgatga aaggatggtt cagcaaaccg atcatcagta agagcaaacc atgggttgtt
    39841 ccaggcacat tacttgtagc aattgcactc gttggtttcg ttacggcacc gaaaaacgct
    39901 gtaacagccg atcaaaaaca actgcaggca aaaatcgctg aaagtaaaaa attagcagaa
    39961 gaaaccagaa acaattcaga aaacaccact agttcggttg accaagcgat ccttgataag
    40021 tacgagttgt ccaaggcaca agggaataag gcacaaaaat tagaactaac ggcatttggt
    40081 gattcagtta tgttggatgc tgcaacggat ctacaagaac tttatccaaa agtagtggta
    40141 gatggagatg tcggacgaca attatatgcc agtgtaccgt acattgaaaa attaaaggaa
    40201 caagatttgt tgaaagacac tgttctgatc ggactaggaa cgaatggctc attctcggaa
    40261 gctcagtttg atagtgtgat ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc
    40321 cgagtaccga cgaagcgctg gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa
    40381 aaatacgaca atatgacgct gattgattgg tacagctaca gtaatgacca tagtgactgg
    40441 ttttatgatg atcaagtcca tccaaatcct gaagggatga cacattacat tcacttagtc
    40501 agcgaagcct tgctaggagg aagttcaagt acgaacagca gtgagacaaa cgtgacagaa
    40561 tgatagctac aaaaaacttc aggcaaaaac atttgttttt gcctgaagtt tttttatatt
    40621 taccgtgttt ttaacattca tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa
    40681 taacgttctt tttgttttcg ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg
    40741 cttcttgaag aaaatacttc tgcatggatc atcttaacag gtcttctttt ttgaagacga
    40801 gtatatttag caccagtacc attgttatgt tcctctaatc gtctagaaag atcggttgta
    40861 tatccaccat aaaatgtctg atctttacag aataatacgt aaaaataatg ttcatttgcc
    40921 atagagcatc tcacgaatct ctgcagtgta ttctccatcc atattgtaag tgatcaatgg
    40981 tggagcgact ttgaatccgt ctaatttgcc ttctttgatt ccttcaacta acaaaatatt
    41041 tgcctctttt ccaggttttg ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc
    41101 ttccatgacc tgaatgattt ctaagaaccg atcaggacga tggaccatag taaagcgtcc
    41161 atttgttttt agtagcttac ttgaaatatc gatcacttct tctaacgtcg tatgaatctc
    41221 atgacgggcg attgccaggt gtttattagg gttcgtttta tttgttggca gccctttgaa
    41281 ataaggtgga ttacagacga ctaagtcaca agaattatgt cgaacgacag ataagctgtc
    41341 tttcagatcg atggtgtgga cagtcatttg ttcatttaaa tggttaagcg cgatactacg
    41401 tcttgccatg tctgctagac gaggttgaag ttcgatagcg tcgatagccg cctgggttcg
    41461 tttacttaaa aaaagtccaa ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt
    41521 tttaggtaca cgggaaaagt gagcaagcag cacagcatct aaagaaaaag aaaacacttt
    41581 ggaactttgg ataatcttga tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg
    41641 aagcatggat tccctccaaa atgaaaaaaa tagtcgtctg tattatacta tttttattaa
    41701 aaagaaccaa ggtggcttgt caaacctcta ttttttatgc atactatgac cataagaagg
    41761 gaaaggaaga gactatgttt tatcgattta tgcgcggggt agttcgagta ctattagcct
    41821 tagttaatgg aaatgcccgc tatgagaata aagaagtttt accgcaagat gagaattaca
    41881 tactagttgc tccccatcgt acttggtggg acccattata tctggcggta gctgcagcac
    41941 ccaaggaatt cagctttatg gcaaaagaag aactgttcaa aaatccagtt ctgcgattta
    42001 tattaactcg atccaatgct tttccagtta aaagagaaaa tccaggacca agcgtcatta
    42061 aaacacctgt aaagattttg aaatcgacaa atttgagttt gatcatgttt cccagtggga
    42121 caagacatgc tacagaactt aaaggcggaa tggcattgat ttccagaatg tctaaagcga
    42181 aaatcgtgcc agctgtatat caaggtccgt tgacacttgg ggatctgttc aagcgtcaga
    42241 gagtgacagt gcgtttcggc gaaccaatcg acttatcaga tatcaagaaa acggataaag
    42301 aaggtatggc ggagatcgaa cgacgaacac aagcagcttt tgacaaattg gacaaagaag
    42361 tgaaccctga tttcaaatat gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc
    42421 ggtcttttga atctactgtc ctgttcagtt cagacttttt caatgaaaac gagggagaga
    42481 cgtcaagtct gccctctttt tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa
    42541 tggatcaaag tgttataata attagaagga cattttttga ggtgattgaa tgcgtttttt
    42601 acatacagcc gattggcata tcggaaaaaa actacaaggt tatgacttga ttgaagacca
    42661 gcgtcatgta ctatcttcta tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt
    42721 gattgccgga gacttatatg ataggagtgt cccagctgtt gatgccgtag aattatacaa
    42781 tgaattgatg gtcgattgga atttaaagga aaagatcccg atttttgcta tatcaggaaa
    42841 tcacgatagc agtacgagac tttcagcggg tacgccatgg ttcagttatt cgaactattt
    42901 tttgcgaaca cagctagcag aagcatttga accagtcgag tttaaaaacg tacaattttt
    42961 ctttcttcct tactttgaac caattgcagc gagattatat tttgaagatg acaatatccg
    43021 aacgatcaag caagcggtag ccaaggtgat agataaaatg caggagctat ttgattcaga
    43081 aaaaaaacaa gtattgatta gccatttttt tgtaatggga agtgtaaaat ctgattcgga
    43141 aacgaaaatc gaagtaggcg ggttggattc gatccctggt gaactactta caccgtttga
    43201 ttatgtagca ctaggacatt tgcataatca agctgctttg agacaggata atgctcgcta
    43261 tagcggttcc ccattgaaat tctctttatc tgagatcaac caaaaaaaag gcgtatggat
    43321 cgttgacctg acaacaggcc tggagttgaa ttttagggag atcgaacccc tgcatgatat
    43381 tgaagaaata acagcgagtt tcaaagaatt gctggatcct gccttttatc agtccgtcaa
    43441 tcgggagaat tacatctata tctatctcac tgatagaacg atcatcccga atatgatgaa
    43501 ccagcttcgg caaatatatc ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga
    43561 acaaacaaag cgcaaacgaa aagtactaga aatgaaagat cctcaaaaat tagcagaagc
    43621 attttttagt gaaatgacgg aagagcagct aactgcaaac caagaaaaat ggctcggaga
    43681 aacgttgcaa gaaatcatgg aaatggaatg atcaagaatg aaaccgaaaa aattacaact
    43741 taaaaatttt ggtcctttta tcaatgagac cgtagatttt tcacgtttga cagaagcacc
    43801 gctttttttg atcagcggga agacaggagc tggcaaaacg acgatttttg atgggatcac
    43861 cttcgcactt tttggtgaaa cttccggaag acttcgctct ggaaaagaga tgcgttcttt
    43921 gtttgctaca ccagacgaag aaacaagcgt gactttttct tttgaacatc agcagatgag
    43981 ctatgaaatc gaacgaaaac cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa
    44041 aaaacaggca gccaaagtaa cgttgaccat ttttgatggg aaaggaaaag aaataaaaca
    44101 aatcacaaaa cgtacagaag cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa
    44161 acagttttct caaatcgttt tactacccca aggagaattc cgtaattttt tggtttcatc
    44221 aagcagtgat aaagagactg ttttacgtca tttattcggt actcaatttt tccaacagtt
    44281 caatgagcga ttaaaagaaa aagcaaaaca acagcaacaa aaattggatc atttagagca
    44341 agagctgcat cttctgcaaa aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg
    44401 gtttgaagcc gttttagccc aatggacaaa ccatcaggca atactcgaaa aacaaatcac
    44461 ggaagaaaaa gaagctctaa cggaacttca ggaaaaacaa aagcaactag aaaattccta
    44521 ctatcgtttt gaaagcttga aaaaaagtta tgaagaaaaa acgatattaa taaacaagca
    44581 aaaggaatta gctgctcaaa aagacgaaat agaggaaaag aaacgatgga ttgggtatta
    44641 tgaatttacg caacgattag tcgaaccaat ccagcgccta caagaaataa aagttgaaaa
    44701 aatagaactg tcacaaaaaa gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa
    44761 ttatgaagag tggcaaaaaa atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga
    44821 agctgctcaa aaactgcaaa acaaacagat gatggtgcca attgtcgaat ccttacgtag
    44881 taaaaaacaa gacgcagaac aattaacaat ttcattggat aaagaagaat tgaatgccga
    44941 gaagctgtat caaaatctcg ctatctatca ggaaagaact cgaattcttc aagaaaattt
    45001 actggatcag gaaaaactgc aagaagatcg tcttgctttt ggacagttaa aatatctaga
    45061 tgaaaagcag caagaaatag aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat
    45121 aagagaagaa ctcaaaaaag caactgaaga aataacaaaa aatagtcagc tattgaaaga
    45181 acagacagaa caagcagaac agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct
    45241 tcagcttttt ttgcgtccag gggagccatg tcttgtttgt ggatcaacgg aacaccccat
    45301 acaacaatta aaacacgaac agccaaataa ggaagatatc ttaaaagtag aacaacaact
    45361 ggcagaagct gagtctgctg tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca
    45421 tgaaaataat cttcaacaac aattatcaga tttagaacaa aaaatcatgg caacgaatat
    45481 acaattggct gaacaagaag aacacttgca acaggctttg aaaaaggcat ttcctgagat
    45541 aacagaacaa aaaacaaaaa caactcttgt agtaatcgaa caatctttag atagacgaaa
    45601 aagcaagatg gccaatgcaa gaatagagct ggaagaaata agacaacaag cagaaaagct
    45661 cgaacaacaa ttagctgtct acaaacagca gcagacagaa aaaaataatc agctccagca
    45721 actgcaagga gaaatcaaaa gtttagaaca acaattgcaa gatacggata gtaactgttt
    45781 agaagaggag atacgacgac ttgaaaaaca gttagaagaa gactcgaatc aactagctgt
    45841 tgagcagaaa gcaggagtgc aattgttgca agatcttcat acgcttcgga cacagcttga
    45901 tcttcaacat gaacaaatag aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat
    45961 agctaaaaag ttagaaagcc agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc
    46021 acaacagcaa gcacaatata aagaaaacca acgtacagtt acttcttatc atgaagagca
    46081 attagtgaca gctgaccggc tgcgtcaatt agaaaatatc cagctaactg agacattccc
    46141 agatacagaa gagatcagcc aacagttaga agaaataaga cgtatgatcg aaataaagca
    46201 agaaaagata tacggtttac aagaacaaaa aattagtaat caaaaaatct acgatgaatg
    46261 tctttcgatt tatcacacaa gccagacgaa attagatgag cttacgcaat tacagcagct
    46321 ttcgcaaaca ttcaacggag aaaatcctaa gaagactagt ttggaacggt atgtgcttca
    46381 ggtttatctt caagaagtgt tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa
    46441 tcgttatcaa tttgagctag cggataccat tggcagttat cgagggaaaa ccggattaga
    46501 aatcaatatc tatgatgatc aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg
    46561 tgagagtttt attgcagcac tttctttagc attagctctt gccgatgtga tccagacaca
    46621 agctggtggt gtagcaatcg atgcattgtt tattgatgaa ggatttgggt cattagatga
    46681 agaagctttg gaaatggcaa tggaagcttt agaaacgatc gaaagtgaag gaagaatgat
    46741 cggcatcatt agtcatgtaa gagaattgaa agaacgagta ttacaacaaa taagaattga
    46801 tacagaaggt agcggacaaa gcaaaatacg ctatcaactt ggataaaaag ggagattggt
    46861 aactatgaaa tggagtattc cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga
    46921 aggccgagtg ttatcggttg tccctgacca agaagaaaac gtgtggcatg cagaagtctt
    46981 gggcagtgaa ttgtatatgg ttgatttgga tggtacagca aaagagatag attactgcca
    47041 atgtccctat tgggaggaac ataaatattg taagcataca gttgctgtgg aactttattt
    47101 acggtcgaaa aatctatctc gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa
    47161 agcaacaagt gagggagaaa tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa
    47221 tcctcaagcg gtgcaacctt tgattattga ataccaagta gaaacgattg agactaatca
    47281 gtatcaccca gagctttcta ttttggcact ttacttgcgt atcggttcat tggcatcacc
    47341 gaaaaaaaca tatatcgtaa aaaacatcta tgatttttta caagcttaca cagaaaaaga
    47401 aacttacact gttaataaac agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga
    47461 agatcaaaaa atactcacgc atcttgcagg aagtgctcag acgcatcaat tattaggtac
    47521 gaacggctta caagtcaaag gaaaactaga taaaagatat ctgcttctcc cagtagaaca
    47581 agcccgcttc ctgttagagc agatgaatca gactacacgt ttgcatctgc agataaatga
    47641 acaaaacgat caaggaatac aattttccga tcaaaacaaa cctttatcat tcaaagtgga
    47701 aaaatttgga gaagattatt tgctgcaagc attgaatgat tttgattttt tctttatgca
    47761 ttatcagtgg ggagttatcc aagggacatt ttattctctg acgaaatatc aacaaacggt
    47821 ttatcaaact tggaaacaat tgattcgccg attggagaaa ccagaagtag tgttcaagaa
    47881 aaaagaatta ccgtctttgt ttaaagagat tatccctctg ctgtcagaga tcggcgacgt
    47941 agctattgcc cctgcagtag aagaggaagt agcagatatc cccgtcgaat ttattttctt
    48001 tttccgaaaa gcaaaaggaa aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat
    48061 ctattcgact gacccgaagc atgaggtctc gacagaacag gcttttcagg tgttacgtga
    48121 cacagtagaa gagaaacgag tacttgatct attcagaatg tatcgctacc agaaaaatga
    48181 aactggttat gaacgtccat tgccaagcgg ggaagaacta tatgcctttt tccgcacaga
    48241 actatctgtc ttccgtcagt ttggtgaagt cagacttgga agaaaattgc gagagttgta
    48301 tttagatgcg cataaacatc aaccgaaaat cgaagtaacc gagaccggtt catggctcga
    48361 tattcgattc gatgtgacag ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt
    48421 gctgagaaat gatgcttttt atacattaga aaatggtcag gttctttctt ttgattcaga
    48481 agaatttcaa caaacaagcc aagtgttgca acaattacgg gaatcgatac gtacagaaga
    48541 aggaacgatc catgtaccta aaaatcaagg attgatcatt caaaatcaat tggaaaaaag
    48601 caatgctact tttagtgaat cgttccaaac gatggtacaa gatctcattc atccagaaag
    48661 ataccaggca cagcttccca aaggattgaa tgcaacgatg agagattatc aaaagcaagg
    48721 ttttcgatgg ttgaagatgt taggtcatta tcagtttggc ggtattttag ccgatgaaat
    48781 gggacttggg aaaacactac agacaattgc ctttttactt tcggaaaaag aagaacggaa
    48841 aagcttctct gctttgatcg tagcgcctgc gagcttaatt tacaactggc aggcagaggt
    48901 gagaaaattc gccccttctt taagtatcca agtaatcaat gggaataaaa aagaacgtga
    48961 agaattactg gctaaagaca cagatatccg agtgacttct tatgccagct taagacagga
    49021 tctagctaat tatcagtcgc aaaaaattga ttatttgatt cttgatgagg cacaaatggt
    49081 caaaaatagc agtacgaaaa ctgcacaagc attgagagaa ttagctgtac ctcagcggtt
    49141 tgcactgagt gggacaccaa tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac
    49201 gattatgcca ggatttttcc cgactaaaac aaaattcagg gaacttgccg cagaagaaat
    49261 tgcgcaaatg attcgaccgt ttattttacg gcgagataaa caaactgtgt tgaaagactt
    49321 gccggaaaaa acagagatga atctatattc tgcacttaca gaagaacaaa aaactgttta
    49381 tttagcttac ttgcgtcaaa tgcgtgaaga aatcacatca atggattctg aagcattcaa
    49441 gaaaaaccgg atcgggatac ttgctggtct gacaagactg cgacaaattt gctgcgatcc
    49501 tcgtctgttt attgaagatt accaaggcgg ttccggcaag ctcgaacaag taaaagatct
    49561 actggtagct gccaaagaga acaaacgaag agttttgctg ttttctcaat ttacagggat
    49621 gctgactatc ttgcaagaag agttagccga gctagggatc tcgacatttt atttaagagg
    49681 cagtacgaaa ccacaagatc ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga
    49741 tgtttttctg atttccttaa aggctggggg aactggattg aatctcacgg gagcagatac
    49801 ggtcatcttg tatgaccttt ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc
    49861 acatcggatc ggacaaaaaa atgtggtaga agtgtggcgt atgatctctg aaggaacgat
    49921 tgaggaacgc atggattcac tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca
    49981 aggtaatgaa gaacagttga cgaaactgac tgaagaagac atccgcatga ttttaagtgt
    50041 aggagaaatc gaggagtaag agactcgaaa aagcgttcag taatgggtaa gaaagccttt
    50101 ctaaagaagc gattgtttat ttgatttaaa aaatagcaag gaggctggga ttccatttta
    50161 tcccagcttc cttgctatca tttttttatt tctattttta aaggttatac aaagattgtc
    50221 ttcattgtct ctaacagctg atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt
    50281 tcaattttgc gttctcgatg gttgaaccaa agcgcttgcc agttagcact ttttgcacct
    50341 agaacatcat tgtcgtaatt atcgccaacg tataaggtct cttctggaag catatgaaat
    50401 tctttctcag ctagctgaaa aatctctttt tctggttttt ggaaacctgt tgcttgagaa
    50461 atgagcatat tgcgggaagg aatccaattg ttcaattgaa gttgcttcag tttttttgtc
    50521 tgatggtcag tcggcccatt tgtgataatt cctaatggga cattcttttc cttcaagaaa
    50581 tcgagggttt ttttgacctc ctcatgcaag cagatattat caagttcttc ctcgtaaatt
    50641 ttttgaaata agaggccatc ttcttccgta atatgagggt agtctaaatc ttttagtgat
    50701 tggctgatcc gatgtgctcg catatattct agtgtccaat ctcctgccat tacttttggg
    50761 aagttttcat cactatgatg gcggaaccgg atatatagtg gatgcatatc tgcatctgaa
    50821 acaagcggaa cgactctttt gaccgcgttt cgaaatggct gctgctgatc atacatcgta
    50881 tcatcaacat caaacacaac tgatttaatc aaaattttac acgcctttcg gttttcattg
    50941 attttttcac aaatccttga ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat
    51001 aaaggttttt cttactatat ttctatttcc ttgacagaat aaggttttta tatttcagtt
    51061 tttttcacta aaaatcgtga aacacgtcaa taagtttgat gaacaaatta atcattatgg
    51121 tagaagtcga ttgatttatt ttaatgcaat ctctgtggaa ttttcgaaag atttattttt
    51181 ttaatacagt ctcaatataa ctattcaaac ataa
//
LOCUS       DACDIH010000014        48140 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDIH010000014 DACDIH010000000
VERSION     DACDIH010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.014"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(50..874)
                     /gene="iolB"
                     /locus_tag="IV281_000829"
     CDS             complement(50..874)
                     /gene="iolB"
                     /locus_tag="IV281_000829"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HAP8779862.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(913..2829)
                     /gene="iolD"
                     /locus_tag="IV281_000830"
     CDS             complement(913..2829)
                     /gene="iolD"
                     /locus_tag="IV281_000830"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HAP8779863.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2840..3874)
                     /gene="iolC"
                     /locus_tag="IV281_000831"
     CDS             complement(2840..3874)
                     /gene="iolC"
                     /locus_tag="IV281_000831"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HAP8779864.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4031..5047)
                     /locus_tag="IV281_000832"
     CDS             complement(4031..5047)
                     /locus_tag="IV281_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8779865.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5149..5346)
                     /locus_tag="IV281_000833"
     CDS             complement(5149..5346)
                     /locus_tag="IV281_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779866.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
     gene            complement(5318..6637)
                     /locus_tag="IV281_000834"
     CDS             complement(5318..6637)
                     /locus_tag="IV281_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011674482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAP8779867.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
     gene            complement(7378..8358)
                     /locus_tag="IV281_000835"
     CDS             complement(7378..8358)
                     /locus_tag="IV281_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP8779868.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(8371..9249)
                     /locus_tag="IV281_000836"
     CDS             complement(8371..9249)
                     /locus_tag="IV281_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP8779869.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(9265..10065)
                     /locus_tag="IV281_000837"
     CDS             complement(9265..10065)
                     /locus_tag="IV281_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HAP8779870.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            11457..11783
                     /locus_tag="IV281_000838"
     CDS             11457..11783
                     /locus_tag="IV281_000838"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8779871.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            11791..12699
                     /locus_tag="IV281_000839"
     CDS             11791..12699
                     /locus_tag="IV281_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8779872.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            13328..13579
                     /locus_tag="IV281_000840"
     CDS             13328..13579
                     /locus_tag="IV281_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAP8779873.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            complement(13907..15226)
                     /locus_tag="IV281_000841"
     CDS             complement(13907..15226)
                     /locus_tag="IV281_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAP8779874.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            15634..16197
                     /locus_tag="IV281_000842"
     CDS             15634..16197
                     /locus_tag="IV281_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779875.1"
                     /translation="MEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVL
                     LAYYQDYRKGWMLPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKK
                     NGNHERARATLLGSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGER
                     YYKVHDNWGDYHKVIPASWSNGTVSLP"
     gene            16277..17251
                     /locus_tag="IV281_000843"
     CDS             16277..17251
                     /locus_tag="IV281_000843"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8779876.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            17440..18576
                     /locus_tag="IV281_000844"
     CDS             17440..18576
                     /locus_tag="IV281_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8779877.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            18713..19057
                     /locus_tag="IV281_000845"
     CDS             18713..19057
                     /locus_tag="IV281_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP8779878.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            19328..20614
                     /gene="efmA"
                     /locus_tag="IV281_000846"
     CDS             19328..20614
                     /gene="efmA"
                     /locus_tag="IV281_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP8779879.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(20752..21351)
                     /locus_tag="IV281_000847"
     CDS             complement(20752..21351)
                     /locus_tag="IV281_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP8779880.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            21755..22876
                     /gene="mnmA"
                     /locus_tag="IV281_000848"
     CDS             21755..22876
                     /gene="mnmA"
                     /locus_tag="IV281_000848"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP8779881.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLIYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            23122..24543
                     /gene="arcD"
                     /locus_tag="IV281_000849"
     CDS             23122..24543
                     /gene="arcD"
                     /locus_tag="IV281_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP8779882.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            24557..25675
                     /locus_tag="IV281_000850"
     CDS             24557..25675
                     /locus_tag="IV281_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP8779883.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(25735..27027)
                     /locus_tag="IV281_000851"
     CDS             complement(25735..27027)
                     /locus_tag="IV281_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8779884.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            27177..27737
                     /locus_tag="IV281_000852"
     CDS             27177..27737
                     /locus_tag="IV281_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8779885.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            27866..28243
                     /locus_tag="IV281_000853"
     CDS             27866..28243
                     /locus_tag="IV281_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP8779886.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            28176..30248
                     /locus_tag="IV281_000854"
     CDS             28176..30248
                     /locus_tag="IV281_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8779887.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(30459..31406)
                     /locus_tag="IV281_000855"
     CDS             complement(30459..31406)
                     /locus_tag="IV281_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8779888.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            31652..32116
                     /locus_tag="IV281_000856"
     CDS             31652..32116
                     /locus_tag="IV281_000856"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP8779889.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(32178..33221)
                     /locus_tag="IV281_000857"
     CDS             complement(32178..33221)
                     /locus_tag="IV281_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP8779890.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(33598..34563)
                     /locus_tag="IV281_000858"
     CDS             complement(33598..34563)
                     /locus_tag="IV281_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP8779891.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            34797..35642
                     /locus_tag="IV281_000859"
     CDS             34797..35642
                     /locus_tag="IV281_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8779892.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            35833..37020
                     /locus_tag="IV281_000860"
     CDS             35833..37020
                     /locus_tag="IV281_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8779893.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            37026..37382
                     /locus_tag="IV281_000861"
     CDS             37026..37382
                     /locus_tag="IV281_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP8779894.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            37384..37722
                     /locus_tag="IV281_000862"
     CDS             37384..37722
                     /locus_tag="IV281_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP8779895.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            38117..39151
                     /locus_tag="IV281_000863"
     CDS             38117..39151
                     /locus_tag="IV281_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8779896.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            39144..39830
                     /locus_tag="IV281_000864"
     CDS             39144..39830
                     /locus_tag="IV281_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8779897.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            39854..40702
                     /locus_tag="IV281_000865"
     CDS             39854..40702
                     /locus_tag="IV281_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8779898.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            40899..41672
                     /gene="sufC"
                     /locus_tag="IV281_000866"
     CDS             40899..41672
                     /gene="sufC"
                     /locus_tag="IV281_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP8779899.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            41685..42971
                     /gene="sufD"
                     /locus_tag="IV281_000867"
     CDS             41685..42971
                     /gene="sufD"
                     /locus_tag="IV281_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP8779900.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            42968..44203
                     /locus_tag="IV281_000868"
     CDS             42968..44203
                     /locus_tag="IV281_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8779901.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            44190..44660
                     /locus_tag="IV281_000869"
     CDS             44190..44660
                     /locus_tag="IV281_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP8779902.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            44665..46056
                     /gene="sufB"
                     /locus_tag="IV281_000870"
     CDS             44665..46056
                     /gene="sufB"
                     /locus_tag="IV281_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP8779903.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKAVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(46151..47293)
                     /locus_tag="IV281_000871"
     CDS             complement(46151..47293)
                     /locus_tag="IV281_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8779904.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(47332..48066)
                     /locus_tag="IV281_000872"
     CDS             complement(47332..48066)
                     /locus_tag="IV281_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8779905.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEKFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
BASE COUNT        15026 a         9358 c         9604 g        14152 t
ORIGIN      
        1 aaaacctaac taaacctaac taaactttat gtatctaaca gcttaataac tacttgcgct
       61 gcaagatcca ttcgtgtttc ttttcattgt ggaatttcca aattcgctca ggtcccgcca
      121 tcacatttaa atagtaggaa gtatacccgt caggtacccc aactggatga tagcccttag
      181 gaacttcgac gacattttga tgctctaccg tcatcgtttc atctagtgaa cgatcttccg
      241 tgtagacccg ctggaaaaca aatccctgct ctggatcaag ctcatggtaa taagtttcct
      301 ctaaaaaaga ctcatgtggc agattctctt gatcgtgctt atgaggagga tagctggaaa
      361 aattaccgcc ctcggtataa acttcgacga ccagcaggct gctggcagta tcgacattat
      421 ccggtaaaat gttgtgaaca agtcgtttat tctgatagag tccacgttct tcgacggtat
      481 tatcctccgc gcgaatcaca cttatcggca aagctttttt cgctggtgaa tagcaaagcg
      541 ccaatcggac attgtctgta gttgccacaa gttcaaacga atgctccgcc ggaagataaa
      601 tactgtcggt tggaattttt tcaaaaacag attctcgtgt tcctaaatct tcataccaag
      661 tgtctgctgc tttaacctta atttttccag tcaaaatgac gatacagacc tcctgttgac
      721 tgccggtttc cttgtaagaa gctccctttt ccagctccaa aatctgaaat tcgatataac
      781 tcaaagcaga attagactgc cgaatatttt ggatttgttt tactccaggt gccagttctt
      841 tgtttaatgg ttgatgcttc aatgtctttt ccaatcaatc cactccttat tttcaatcac
      901 aaaaagcaat atttaatact ttctgatagt ggcaagtttc gcttgtttgg ctttgtaagc
      961 ctcctgcatg gctgctttac ccgatacctc tgagataccg acgttccacc agctgccgtc
     1021 gtagccttct gtcatggttt taggaagtac ctttatctca atcaacgtcg atcgcgtttg
     1081 cttcttcgca tcctcgaccg ctgcttgtaa ttctgcaatc gagcttacac gatacgtttt
     1141 tgcaccatag ccttcagcaa ctttggcgta atcgatgttc atgatctcgt ccttggtatt
     1201 tctgaattcg gttccttggc tagctccgcc attatccatc tgtaaattat tgatacagcc
     1261 gaaaccagaa ttatcgaaaa gtacaatgtt gattttttta tcatattgca aactagaaac
     1321 cagctcgcta tgcaacatca agaaactgcc atcgcctact aaggcataga cctcttgttc
     1381 tggtcgagcc attcttacgc ccatcgcacc agcgatttca tagcccatac aagaataacc
     1441 gtattccaaa tgataggtat tctccttcac agggttccat aagcgctgca tatcccccgg
     1501 caatgaaccg gctgaaccga tcacgatcgc atcctcatca acaaaatcat ttaaatgaat
     1561 gaagacctct gtttgcgtca aattcgtagc caacgcatca gcatattcat tcaagatttc
     1621 ttgagaaaaa tgatctttga tttctggcgt aaaatcttta cgcttaaaat gcgcggcttt
     1681 taaccgattt cgctcactga tccattcttc cttcagttca tcgatcttct cgccataggc
     1741 ggtttggtaa ccttcgatca ttggggcaag ctgctccaag gtcgctttcg catcaccaac
     1801 cacttgaaag gcttccagct tagcggcctg ttgtctgctt acgttaatat tcatcatttt
     1861 cgcttgttca aaattaaaaa tacttttaga agctgaggta aaatcagtgt agcgtgtgcc
     1921 gatccctatg atcagatccg cttgctgcac agctttatta gcagccgaag ttcccaaaat
     1981 ccccattccg ccgagattgt ttttaaaatc ccacgaaaca gtcgatttac ctgcgtgcgt
     2041 ttcaactagc ggaatctgac aatctcttga gatttgctca agaatctcac cagcctccga
     2101 atatttgcag ccgcctccta cgatgatgac aggtcgttta ctggccttta ttcgttgact
     2161 cgcttcagca agctcacgag cggtcggaat cctgcgatca atataatgta ctcttttttt
     2221 gaaaaattcc tccggatagg cataggcttc tgcctccacg tcctgcggca aacaaatcgt
     2281 tgccggaccc atcgttgccg ggttcgtcat gacttcaaag gcccgcaata acgcggacat
     2341 caactgctct ggtcgattga tgcggtccca gtagcgagaa accgcttgaa atgcatcatt
     2401 tgtcgaaaag gctacactgc ttgcatgttc cagctgctgc aagaccggat caggctgacg
     2461 tgtagcaaac gtatccgccg gtaaaaaaag tacggggata ttatttgcga atgccgtacc
     2521 cgctgctgtt aacagattgg ccgcgcccgg tccaacagat gttgatacgg caaaaatttt
     2581 cttgcgtaaa ttctgtctgg caaaggcagt agccgcatgg gccattccct gctcattttt
     2641 tccgctataa cttttcaaat gtccgggctc taattccagc gcctgaccga tccccaaaac
     2701 atttccgtga ccatagacat gaaagatacc ttcaacataa ggcgtttctt tgccgtcgat
     2761 gctgatgtat tgttgattca aaaattttag taatgcttgt gctgttgtta aacaaaccgt
     2821 cttttccatt tttttcacct taaatccgag cgatcatttc gccgtatatt tctttctcca
     2881 atgtaataaa atcttttacc tcctgagtcg tcggtaaaga atctgaacag ttattgcttt
     2941 tgaccatcat tgatgcttcc gctgagccaa actctaaaca atcgatgatc tcccagcctt
     3001 cgaataaacc gtataagaaa cccgagccgt aaccatctcc gccgccaaag cctttgcgtg
     3061 cttccaccgg aaacggcttg attgaaaact gctgctgatc ggccgtataa gcagtcgatc
     3121 ctttcattcc atgtttgatt acgacaatct cagcaccaca actgtgccaa aaagcagcgc
     3181 tttcattatc tgacatgtta ggctggatca acttctcagt tagatcgaac tcttctcttg
     3241 aacccatgat aatgtctgat tccttagcaa tcatcgaata gtaaattgaa atatcatcgt
     3301 tgttcttcca gttataagca cggtaatcaa tatcaaaaat aattttgaca ttattccttt
     3361 tcgccagcat cactgccttg attacggctt ctctggacgg gctctctgct agtgcagttc
     3421 ctgagattaa tagtgctttt gaccgtttca agtagtcctc atcaatatcc tcaacgctaa
     3481 gctgcaggtc tgcggcttga tttcgataca tcaaaatatt actttccttt gatgagagca
     3541 tttcggtaaa agtcagcccc agcttttcac catgctgcgc tttgctgatc tgagacgtat
     3601 cgatgccttg ttcattgaaa taatcgatca catattcgcc aaactgatcg tccgagactg
     3661 ttccgataaa accagctttc atgccatgtt tagtaacacc cacagcaata ttggcaggag
     3721 atccgcccac aaatttttca aacatatgga cctttttcaa tggtttaaac tcttctttga
     3781 cttgatcatt gtaggcagga ttaaaatcaa tagcgactct tcctaaaagc agcaagtcaa
     3841 attcttttga ttggtcaaac tttatataac tcaatatatc cacctcttta tccgttcttc
     3901 tatttctgag gcaagtatag cacgccaaaa caaagcgtgc aatatttatt ttagaacgca
     3961 atattgcgtg ctgtttgaaa atgacggcaa aaaaaacgtg aatcgcctta catcacgatt
     4021 cacgcctaga ttaaatattg tattttgtct taataattat atcagtaaat agcgcatctt
     4081 ttgtaaccgt tttattattt tgcagctgat tcgctaacat cagtaatgaa cgatagcctt
     4141 gaacaaatcc atcttgatcg attagaaaat caacctgatc gtttttcagc atctcgatat
     4201 tgttcttagt caaatcataa atgattacaa agggccgctt gctttcatcg attttttcca
     4261 acgctcgggc aattcccgct tgtccgccag aaaagacgac tacgccatct aagtttgaat
     4321 aattctccaa ggtgttgctt aaaatctttt caacctcagc actcgaatca aagctgctct
     4381 gaacacccgt cagctccagt tcaggaaacg actctttcaa ttcttcaaca aagcctgcca
     4441 cccgtaagct gttgacgcta tttccaaagt agccggtaat cgctagaacc ttgccctttc
     4501 cttttgtcag catacctaac aatcccgcag ccgtttttcc gcttttgaag ttgtccatcc
     4561 caacaaaaca ctgtcgattc gtaccaagaa tatccgaatt aaaggtaatg atgaggatgc
     4621 cttgatcggt caattgatta attttttctc tgataccatc actatctact ggcatgatcg
     4681 cgatcccttt tactccaagc tccaccaatc gatccaacgc ctgcagctgg gccgcttcat
     4741 cgaccccgtc tacctcttct aaaaaacaat caatgtttcg cttttttaaa tccccgataa
     4801 tagattccag acctgatcgg atcggaatca taaaagaaga tttcgccaat tgagtgacaa
     4861 tccccagctt gagcgttgtc tcttttatga ccttcttttt ctttggaaca tagttcattt
     4921 ccttggcgat tgctctgatt ctttctgcaa cctcaggatt gatccttcct cgatcgttca
     4981 atgcccgatc cactgtcccc cttgacactc ctgctttctc agctatttct ttaattgtac
     5041 ccgccatctt gcctctcctt ttttcctatt gtgtttattt tatcacaaaa cttaagaaaa
     5101 ccaccggaga atgaattctc cggtggactt tttgtatgat taaccttctt attgaacatc
     5161 actgatctta aactctattc caagggcgtt tctataatat tctggataca tctccttccc
     5221 acatttttca caggtaaatt ggggaggatt gttcggatca tttgcaatat ctgcatcatc
     5281 taaaaatttt actacatcca ttgggattga ttctttttca ccacaactta gacatataaa
     5341 tttcacgttg gcgttcccaa tcccagcgaa ttgctttaaa agttttttct ttttcttctt
     5401 catactgttg cctgaattct ttttggtcaa tttttcgatt ctcctctcgc ataaaccgct
     5461 ttgcatggtt atctttcgca tatttaataa ctaaccgtcc atttttgcgc acactcatcc
     5521 caacgaattc atagtactca ccgcaacaag aacattctaa gggattttca ccaaattctt
     5581 cggttattct ttcttcccag gattttggtc ttaaggcctc attcagattg ataaacagct
     5641 tttttactcg tttctgatac tccttcatga cctttttcat taatgttttg attctacgac
     5701 tatagattcc atagcgacga atcgttttga aattcttatt ttgaatatgg cgaatcagtg
     5761 cagctatgaa ttcgtctata ctcatggtca tcgtctcttt agtattcgtt cgtttgtcca
     5821 tgtagttgaa catgacagtg tcgccatcat acattaagat tcggcttaat gcgatcgggc
     5881 cacgcttcat ataacgactg atgtattgga gtagtgactt tagatttgtc ctgctccttt
     5941 taggagcatt tacgtaaaac ccttctccat tgttcttgta cgcagcttgt aatcttgatt
     6001 gaactctttg cttgtcccac tcacctaacg ttcgtctaat tagtttcaat acagcatttt
     6061 tccagtattt tcttaacata gaaaaaggta aataatcgta ttcttcccaa cttccatctc
     6121 ttgttaagcc acccatagta acaaccatat gaacatgcgg attaaattct agttttgatc
     6181 caaaggtgtg gattgtagct actatacctg ccttcacctt tcttttctca aaataggcaa
     6241 gaataatttt tgcggcttca tccattagtc ctttcagtaa ggaatctcga tagttttcca
     6301 tcataaagat ttcccgtaac ccctcatcga tcgtaaagat tacgtgccga tgtgtcactt
     6361 taaacatatc attcgccaca accgctgacc acctttggct ttcacctgtt gcacacgttg
     6421 gacagaactt tccacgacat ttcaagggaa caaactttgt ttgatgacat ccctcgcaga
     6481 caaacagtcg gtatcctttt tgaatatctc cacaatatct aaacttgttt acttcccgtt
     6541 tgacgactag ccgaatcttt cgcccgtatt tctcaacaaa gttatcccaa ttatggtgtt
     6601 cgtcaaaaaa gattgcttcg agaatattcg ctttcatagt aatattttac ttgttcaaaa
     6661 acaaaatgaa aagggctaaa agactggtag tgcagccttt ttagccctta actttttata
     6721 ctttctttac tgtctaaaaa atatgcggcg cacagaacgc gccgcatatc ggactaagtt
     6781 ttattcaaat ttgtcttcgc caacacaatt tgacgaagag ccttttcttt caggaagact
     6841 tcggtaattt ttaggagttc tacctattct tttaaccgtt tattctcttt ttctaagcgt
     6901 gtaatttcat ttctgcgaat ggatttcatg acttcctcta ccgagaaaag tacgttatcc
     6961 atatttttca acttcttgaa ctcatcaata cacgctatta gagcgagctt ctagctatag
     7021 actaacttct ttaacagaaa acccttcttc cgttaccagt tttacagcgg taattttaaa
     7081 tatttcatca aatgaacgtc ttggcataat ccagatcttc gctttctctt aatttggtgt
     7141 caattttagt ataacccgtc caacttattt tttaactaag tgcccggttt ttcttgacaa
     7201 ctccacccaa aagttagatt ttttggtcta agttttaggg tcgcttcatt actagaattc
     7261 aacaagagat gttttttatt ttatttaaat tatggggtca gtttccattt gattacaaac
     7321 ttctttgttt tcttttttta aacgcaataa taaaggtttt aatttcagcc tttttaatta
     7381 gaaaatcaat tttcccaaat tattttcttt agctttctta acgatttctg tcgcaactac
     7441 cacatctaat acagccgaac caactgtttt gaagatcgtg atttgttctc cacttgtccg
     7501 accgatcaat tcttcattga tcagttggcc tagttcccca tgatagctgt ctctttgaat
     7561 atacccatct tgcagcggtg aaatgaagtc cccagcttct tttagaacac cgtccatggt
     7621 atcaaaaata acaacatctg cggctttgat tatttctcgt ggaatttcac acatttccgg
     7681 tgtgtaagca ccgactccat tgatgtgcgc accttttttg acccactcag cagagaatgt
     7741 cgcacgtttt gaagtggtca cacttgtaat gatgtctgct ccttcaacgc attcttgact
     7801 ggtttttgtt gggcgcatcg ttacagaaaa ttgttgcatc attttttcag caaattggct
     7861 ggcacgctca aaatcaatat caaaaatccg aacttcttct aatttacgaa cggtcaacat
     7921 tgcttctaat tgtgactgtg cttgtccgcc ggttccaatc agcgcgccaa ttttagcatc
     7981 ttctcttgca agaagttctg ttgctgctcc ttgaacagcg cctgttctta attgtgtcaa
     8041 ataagtacca tccaaacaag cggataccat acccgtttct ggatccaata cgatcatcgt
     8101 tgctggtaca cttggtaagt ttttcttgat gttttcagga tacacggaaa cgattttgac
     8161 acctagcgcg ccttcgccac cttctacata agcaggcata tataagcttt gtccgttgct
     8221 ttttggcaca tcgatattgg tgcgaagagg cacacttgcc tttcctgctg aatacaatga
     8281 cagtgctttt ttgtccgcat cgatggcttc tctcattgaa aaacttttga taatatcttc
     8341 ttttttaaga aataacattt gaatcgctcc ttaattagct gctgtaactt tttttaattg
     8401 actgcccaac atcgtatatg cactgtatag taagaccaaa atcacgatcc atttcaggat
     8461 ataggtatct aaagatttga ctaggaatac agcgaccaat acccccaata caccgaaggt
     8521 tgaagtaaaa agtgtgattt ttctgctgta ttcattaaat ttaatgaatt gaacactgcc
     8581 gatcggtact gagaaggtac aagcccccat cataatagga aaggctgcaa ctggattcaa
     8641 gcctagcaag tagacagtta ccatcgttaa cgcataagaa ccaatgccga tattgttcaa
     8701 tgcaccatag acaaagagta aaaatcctgc caagattaat tttccgccat acaattcact
     8761 ggctgttccg tttgatggaa tccagttcat ttgtcctgca aaaatcaaca atgcagcaat
     8821 gaccaatcct atgccgacaa acagtttgat cgttttctct ggtaacttca cgacaaatct
     8881 tggtcctaaa tatgcaccga taatttggca gataatacaa acagctagtg tcttgatccc
     8941 tacgttgatg gcagtgatgt aagacaatgc catgacggct accggtacta cacattgggt
     9001 attcaacgtt ccgggtagtt ttttcatgct gacccatttc aactttggat aaaggacggt
     9061 tccgatagca aagtcggaaa cgccaaatgt tgaaagaaag aagatgataa atgacgtgaa
     9121 gggcaacaga cgcatatccc ctggttcttc tttgaaattt tgtttgtttt tgaaaaggtc
     9181 tcttagaaag taaataataa aatatagatt gattgcgaca attagtgtca gcaaaatatt
     9241 ccgaatcata aaaaattcct cctactagta aatctttctg attccctcaa agccctcacc
     9301 atattttgct ttaagttctt caggagattc tgcaatcact tttagtgtat atgcatcatg
     9361 tttgacatat tcttttgcta gattgaccat ggcgtggttt tttgtccaag tgacatcggt
     9421 tgattgcagt tcttcgttaa tggtaccaaa cacgacttct tgctcatcgg cgacgatgtt
     9481 gatccatctt gctccaaagt cagcaagttt gtctgtttca aaaccatgaa tataatactt
     9541 ttctaaggga agatcatatt catgggtaga actgaataaa atcagtacaa acttatcaac
     9601 tcttttttca gcttgctgca atgcctttag tagtgaatcc gtgaaattct catgccaaac
     9661 ttgaatgagc aaactttctt ttgcgttttt caccaaatgc tctactttat ccagaacata
     9721 ctcgatacca tcaactttcc acagcatttc ttcttcatct ttttctttga tcatccctag
     9781 attcttggta agaaaagaaa gatcattttt aacacttttt tctaatttct ctaaaaactc
     9841 atttgccgag atggcatgat aaagtttagg ttctgattta ttaacgacaa tcaaattctt
     9901 ttttaatagt ttttccaaaa tattatatac tttcgagcgt ggcacacctg agattttact
     9961 tacttcatat cctgtaagat tacctttttc taatagtgtg gtatacactc ttgtctccat
    10021 ctctgaaaaa tcatattttt tcatgatttg aataatcgtt tccattgatt aatcctcact
    10081 ttatttttag tagctactac ggtaacaact ataaattctt tttgattatc tgtcaagagt
    10141 tagttcaata acacacatat aataattagc ttaaaaacaa tctttttatt aaaaagaaac
    10201 attataatat gaatgtttgt gaaaaaataa tacaacttag tcattcttta caacgattta
    10261 tagaaaaaca acctctttaa atataaaagg tacgggttac tgattacttg taaatcttta
    10321 cgttccagtt ttattctttc taaaaaatat actcacttct tttcttagac ttcttttttt
    10381 gattctcctc tttatggtct tgtccttttt tgccttcaaa atatttaaat aaaccccttt
    10441 caaaagcatt tctaggtttt ggacaataag gaatgacttt tttcttcata taaatacctc
    10501 atttaactat ttgatatcat cacattatca aaaaaaatag gttttttatt aaaactatct
    10561 tgacctttcg ggagatttca ctgtgattta tttttattcc catacttttt tcgttggtca
    10621 ctttcgtata cctaatctaa gcgatcatcg gcgacggtgc ttttacaacg aatggcagga
    10681 caaaacaagg ggcggaaaaa cttttttgcc tatttcataa aaaacttttt cctacagcaa
    10741 tcccttgttt tgtcctacca cacgttgtga acccaaagca cgccgacgaa cgcttagcta
    10801 tacgaaaatg acctccaaaa acaaaaaagt gaggtgaata gaaacaaaaa gaaattgaaa
    10861 tctcccaaaa tgtcgtccgc ctcggagaca acttgctgct gaaaatggtt ggttaattca
    10921 tttaagtatt aaataagata aatcatcaaa ttaatcggaa aaatagcatg attggcctta
    10981 tcaaaaataa actgtaaaaa cttatatttc tatgagtggt ggaacaatgc taataccaaa
    11041 ttaatttatc gattacccac ctgcacaaac caaagatagc attattagga aacgaatcaa
    11101 tcagcgaatc cagcattata atgaggatac taaaaataaa gttttcataa ttataaatcc
    11161 gccttcctac gaaaggcacc aatgaggtga tgaaaacctt agagcgcttt ctttcttttt
    11221 ttattccgtg ttaccctttc cttgcaggtc cctggtacac tacagaaccc gaggaggtga
    11281 gtggcggggc tgtatagcgg caaccccgca taattatatg gtgtcggtca tccgttaagg
    11341 catcaatgaa tggcgcaaac ccgtagcccg taaacttatc tcttccgaag ttgactcgat
    11401 ttcgccggat gaccagtccc taccaaacct gtactatttt tttacaggag gttgctatgt
    11461 tcaaaatctt tcgtaaaaat tgttgtggct tggatgtcca caaaacgtgg atctatgcgt
    11521 gtatcggaat cacagatgcg aatggtcgta cagtgtataa gcaagctcgc ttctcttcct
    11581 ttacaaaagg tctcaatgaa ttagctgaat ggcttgctaa atacaactgt aaggatgtgt
    11641 gtttggaatc ttccggtaaa ttttggattc ctgtttttaa tattcttgaa aaaacttgtt
    11701 ttgtcacact ggctcatccg aagtacacca aaccccagaa aggaaataaa acagatcgca
    11761 aagatgcaaa aatggatttg tgatctcttt atgtgcgaca tgatcaaacc cagttttatt
    11821 cctggtcttg aaatccgcca gcttcgtgat ctaatgcgtt atcggacgaa actgaccaac
    11881 atgaaaacaa gtgaaaagaa tcgtgcttta aactgcttaa ccgtttctaa tctgaaacta
    11941 gatgatgttt ttagtgatgt gttcggaaaa tcatccactt ctatcatcca atacttgtta
    12001 gaacatcccg gtgaatcatt tgatgttcga ccatttatcc atgcccgttg taaaactccc
    12061 gttgaagaaa tccaagcggc agttgagggg gcgatttctg tacctcaagc gattaagctt
    12121 cgccaatgcc tcaaccatat taacgagatt gagaagcatc gagaagagat caatctagag
    12181 attcaagctc ttgcagaacc cttctcccct gttttggaac tacttcgtac cataccgggc
    12241 ttagacactc agccaataac agcgattgcg attctttcag aaataggacc ggatatgtcc
    12301 gtgtttccaa cttcgaaaca ctttatttct tgggctggat gctgtccaag aaacgatcgc
    12361 agtgcccgac gaacaaaatc aacacgtatc tctcgtgcag ggcagtcgtt gaaaccgcta
    12421 cttgtacaga ttgccaatgc tcttctcaga tccaaagaac atccagaatt taaaaatcgc
    12481 tatcgtagaa tcaaagcgaa tcgtggacac aagaaagcta tcattgctat ttgtaagatg
    12541 ctcctgaccg ctatctggaa tgtcttatca aaggtggaac cctattctgc taaaggatat
    12601 ctggagccaa aacctgcgaa agaagagaaa gttctgacgc aatcccaagc tctggacctt
    12661 ttaagaaaac gcggatatac cattatagat agtgcttaaa ccaaagttac gttgattctt
    12721 cctaaataga ctcgttgtct tccatttcaa aaattttgaa aacggtaggc ttatttagtg
    12781 taccgttctt caggtatctt caaatagatt tattttcaaa cttatttcct ttcttctttt
    12841 actctcctat agcttagcat ttaagtgggc actcgtgtca ccttgctaga tagctatgaa
    12901 tgccgtaata agcctcctag tatataggca gactgccaat tataactgat tcaatccaca
    12961 ttatgataaa atccaccttt tcgtttcata tgtgttgaaa tatcacatat gtcaaaaggg
    13021 cacgagtctt ttcattcagt gctttgtaaa tactcaaaat tattcctttt cactttaaaa
    13081 aaaagataat tagtacaaaa tcagaaatga ggaaagtatt aggatggact agaaaggagt
    13141 tgttgaattt gttgcaacag attggtcatt tatcaattaa aaattaggtg aagttttatt
    13201 catcactgca gctgctgttt cccagagaat aaaacgtcta gaagaaactg gtcagttact
    13261 gtattatcaa gcagcaattg actacgaaca agcttgatta ctaaccaaag cgtttatcca
    13321 cctgttgatt aaatcaaatc aaaaatcagg tttttatacg ttcataaaag caatctccag
    13381 tgtactcgaa tgtgactgta tcataggtcc ttattcgatg ttaatcaaaa tggggtttcc
    13441 ttctaccgaa tcattggaca gttttatcaa tgatttatca aagtttggac gaaccaacac
    13501 ccaaatcgtt ttctcgactc ttgtttcctt aatggtttat cagttctgga atgtttatca
    13561 tgcgctttta ttaaaataga aaagagcttt tttcatgcga caaagcagtg atcatttatt
    13621 ggcacatgca atctttgtgg atccattttt tgaaaatacg atatttagtt ttgacccaaa
    13681 aatctttaaa tacgtatttg atacctgaat aattcatagc tttaattctt tggcacattt
    13741 tattgaaata tgtatcacta atatccctat caactgattt tgactaaaaa gttacttcaa
    13801 acattcactg ttcaaaaata ttgaggaata aattttgggt gtaaggcatt ttgagcatac
    13861 ttctccctgg ataacttccc agtaaaaaaa tcgttagatt gttggattag atccattgac
    13921 tggctcgcct caggcgagca aagaccctgt agaattcatt ctgatacaca tgataactag
    13981 ataatttgag atagactcgt ctacccgttt ggactaattt cccagcaact ttcagaaact
    14041 tcaatcgaat agaatgaatg gtcattccct tttggacttc ttcaaagcca atcgttctta
    14101 agaagttgac taagttgtaa gctatcaagc tgagcatcat tcggacatga ttctccaaaa
    14161 agcgaggact gtctgttttg tcaaagtaaa agccagcttt cgcttcttta atgaagttct
    14221 ccattgtgcc acgtttggca tagatagaaa agatggtatc aggagaaaca ttttctgata
    14281 gattcgtcac gataaattca tgtcgaaaga gtagttcgcc cgcttcacgt gttgagcgta
    14341 tacacacgcg acgactttgt gaccaggatt gtgcttgata agtggtcgag aagtactgaa
    14401 cttctcgttc ttcccacttt tgattatcgc cataaagtac tgatttctca gctatttgac
    14461 ctagtctacg attattcttc agtcgaataa cataatgact cttttttgat tcacacgaat
    14521 catacacacc aggtgtagcg aacccgctgt ctccacgaac caagatgtca gtgtttggta
    14581 gagagtgatt atagtgctct aataaaggtg tgagaaactc ctttacgcct tttgatgtat
    14641 attgatttcc tgaacgtagt tcagctttta agaagtcacc agtcaatccg tcaaaagcta
    14701 ccaatggatg gtaaccatag gtttggtagt gtgtattata attcgtttgt tcttggtgac
    14761 caaatgtatc tgaatgggtc gaatctaaat caatgattaa ttccgtatca ttacggatga
    14821 ggcgtgcttt atcgataagt tcttggttca aagcttgaag ttcatggata ttctcctcag
    14881 atagtcgatc taaaaatcgg gaaagtgaag attgagaagc gagttctttc ctatctaaaa
    14941 cagctttaaa cacaggatct tgtctcagga gattagctgc agaatcagct gagtaaccag
    15001 caattaattg cataatgaac tgctcaagta tggataagtt gtcatgagta aaatacgctc
    15061 gttcgtcttc aatgtgaatg tgttgcttag ccagttcaga aaaaccaaag atgttcatca
    15121 gctctttcac taggacgaga cccgaatcac tcgataattg accacctgta tgagaaatag
    15181 tgatatttga attgaatttt acttggtttt tgtgtaagct agtcatgaaa agaactcctt
    15241 tcttttggtt ggtttagtca ctttaaccat agcagaaagg ggttcttttt tcatcactta
    15301 acgggtgaag atgaaaaagt aattgtaagc tgccgtgagg cagtttcaca tggttttaat
    15361 taaaagtatg aattattcag ggtagataaa aaaattaaaa aattagccag cactcagcag
    15421 ctgcagtcaa gaaaaagtaa attgcttttt ttagctctgg tttttgaaga tcaaagttat
    15481 gtgattatcg atcaatcagg acatccggta gaatattctc cagcaaaata tacttatcaa
    15541 gagggtcttt cttgtaaaaa atggaagctg atcaatgaag agccaatcga gttatctaga
    15601 tggatcaaca gaaaagaaga tattcccgtt ttgatcgaag aaaaaaggtc aggaaaggaa
    15661 ctcgcaaatt gctgggtggg attgccagaa gaacgttttt tgaggtataa gaaatgggct
    15721 accccctcag gctatctttg tggtacttac gcggcagcag ttctccttgc ctattatcaa
    15781 gactatcgta aggggtggat gcttccaaat gagattcgca agaaaaatac ggctgattcg
    15841 ttggttttga cgaaggcatt gcgtagccag atccaaccac taggacttcc tacgattcct
    15901 tttcaggtta gtacagggat ttctagtttt ttaaagaaga atggcaatca tgagcgtgca
    15961 cgagcaactc ttctaggttc atggcagcga gctacaaaac gtatacgtga aggcaaacct
    16021 gtgatgattg gtattttgaa agtacttggc agtacatatg gcaatcattg ggtgacagcg
    16081 tacgcttatt ttgaaactga aacaggagaa cgttattaca aagtacacga caactggggc
    16141 gattatcata aagtcattcc tgccagttgg agtaacggaa cagtttcact tccttaagaa
    16201 tttgctgaaa ttaacagaaa atcttggaaa aatatgttaa actatcgaag aatagagaag
    16261 ttttggggag gactacatga gtaataagta tcaggatgac acgttgaaga tttttagttt
    16321 gaatggcaac cgtccattag ctgaaaaaat cgcgaaagtg ttcggaacag aattaggtaa
    16381 aagcgtcgtg aagcaattta gtgatggcga gatttccatc aatatcgaag aaagtattcg
    16441 aggggatcat gtgtatatcg ttcaatcaac gaatgcacct gtcaatgatt actatatgga
    16501 actactgatc atgatcgatg caatgaagcg agcaagtgcg aaaacgatca atgtcgtatt
    16561 gccttactat gggtatgcta ggcaagaccg cacggcgaaa cctcatgaac cgatcactgc
    16621 gaaattgatc gctaacttga tcgaagaagc aggggcaact cgagtactga ctttagatct
    16681 gcacacggtt caagtacaag gattctttga tattcctgta gataatttat ttacgatgcc
    16741 gttgtttgct cattattacc gtcaattagg tctgacggga gatgacatcg tagttgtttc
    16801 gccaaaaaac agcggtgttc agcgtgcacg tagtctttca gaatatttga acagcacatt
    16861 agccatcgta gatcatgcag atgatgcttc aactgagggt ggttatgtga tcggtgatgt
    16921 tcaaggaaag acatgtatca tggtagatga tatcttgaac acaggagcaa cgcttgcacg
    16981 agcagcaaat gtattaaaag aaaacggcgc aaaagaagta tatgcctgtg cgtcacatgg
    17041 attattgtcc gatcatgcga aaagtatttt agacaatgca ccaatcaaag atatttgtat
    17101 cactgattct gttttcacag aagaaaatcg tcatccagaa aacttaacaa tcgttacatg
    17161 ttcagaatta atgggtgaag cggtcaaacg catccatgaa aatacaccga tgagtccgtt
    17221 attccgtctg gaagaaaaag aattcgaata aacaaaaaaa agagggaaag aagttgtgac
    17281 agaagtgagc agcttcaagg agctgcgaca agacttttgt cacagctcct tttttctatg
    17341 cccatgaaac gtcctctaga caaaaatgag ctcttctttg gtttgttcgc tattttatgc
    17401 tagaatgtgc ctagcaataa tttggaaggg atgaatgaag tgaaaactat ttatctggat
    17461 catgcggcaa ctacccccat gcatccacaa gtaattgaag cgatgacggc aatcatgcaa
    17521 gacacgtttg gaaatccttc aagcatacat ggatttggtc gccacgcgca tgaaaaattg
    17581 gaaacagcaa gacaaatcat tgcagacagt ctgcaagcaa aacctcacga gatcatcttt
    17641 aacagtgggg gaacagaagg ggataataca gctatcttag aaacagcgtt ctcgcgtcaa
    17701 aaagaaggca gacatctaat cactacagct atcgaacatc ctgctgttct taataccatg
    17761 aaatatttag agacaaaggg gtttgacgtt acatatctgc ctgtagatga aaaagggaat
    17821 ctgtcgatcg aagaggtcaa aaaagcgtta cgagaagata cgatcttggt ctctgtgatg
    17881 tacgggaaca atgaaatcgg aaatttgatg ccaatcaaag aaatcggtga acttttagcg
    17941 gatcatccag cttatttcca tacagatgcg gtacaagcgt acggaaacca aacgatccat
    18001 ccagaagaat taggaatcga tttattaagt atctctgctc ataaaatcaa tgggccaaaa
    18061 ggcgtcggtt tcttatataa acgagacgga gtaaatatcc ctgcattgct tttaggcggg
    18121 gagcaggaag aaaagcggcg tgcaggtaca gaaaatttag cagggatctg gggtatggca
    18181 aaagcagttg aactgctgtc acctgaagaa cgtgaagcac gtaacaacaa atataaagga
    18241 ttccagcaat tgattcttga tacattagaa gcaaacgaaa tcccttatca agtaaatggt
    18301 gatttaacga ataaactccc tcatgtgttg aatcttcgct taattggcga agcaaatgat
    18361 ttgctactga tgaagcttga tcttaatggg gtcgctattt ctactggttc agcttgtaca
    18421 gcaggaaata tcgagccttc acacgtatta gaagcaatgt atggagaaga tacaccgata
    18481 ctaaaagaat ccatccgtat cagctttggt ttgggaaata ccgaagacga catccagtat
    18541 ttccttgatc aattagttcg tgctgtcaaa aaataactag tgaagtcatt cttatatgaa
    18601 cagatgtctg agagaacgcc ttcatttttt agggaactta actgaaaaac ctagtaaaaa
    18661 tcaggataaa cttttataat aaagagaaac taattttatc gaggtgaaaa tcatggcatt
    18721 tgaaacaaca gcaacggtac tgggcgcacc aattagctat cgtttgcatc ctgatgcaaa
    18781 aagatataca ttgcgcgata atggatttac agaaacaaac ggaggcaatt atcaattgat
    18841 ccgaccatta gatgccacgc cgcaaagcaa agaaggattc aaattaaaaa tcactgtatc
    18901 aaaagatatt caaacattga aaatgtcgat cactacagca aatggattga aagcagtcaa
    18961 tattttcaaa gatccaaaac agaaaatgag ccaagataaa ttttattttc tgatggatgg
    19021 gatgattagc cgtgggttat tcgaaaaagt cgaatgatct gtggatataa aaagacaact
    19081 agttttgaaa cggcctattg ttagatgact aactttgtaa ggacgacgca aattagttgt
    19141 cttttttatt tttaaattta acacatgttg ttatttttaa tattttgctg atttattgtt
    19201 tctaagtaaa gaaatttaaa ttgaaacgat tgttatttag ggaaaaaggt attttttgaa
    19261 aaaagaatcc cgtataatga ttgttgtgca aacactatac aaaatgaaaa agaaaagagg
    19321 gatgtttatg gaaaatgaac agtcggtcgt tttaacgaat tggaagagaa attatttgtt
    19381 ctttttatcg ggacagtttt tgtcaggaat cactagtatg gtcgtccaat atgcgatcat
    19441 ctggtatttg acaagagaaa ccggttcggc gacaattttg agttttgcta ctttactagg
    19501 aatgattccc atggttttac taagcccatt tgtaggaccg ctagtggatc gctgggataa
    19561 aaaagctctt ttgattgtca cggatattat tgtagcgatt ttcgcgctta ttttagcagt
    19621 agtcggaacg atttcagaat ccttcccgat ttggctagtt tttgtgtctt tgtttatgcg
    19681 ttcggtcgca caaacattcc aaatgccaac gatccagtcg attatgccga caatcgttcc
    19741 atcttctcac ataacaagga cgaatggaca gttaggaatg gtccaatcag cgaatttcat
    19801 cattgcgcca gctctaggtg cggctttgtt ttcagtcgtt cctgtcaatt accttatttt
    19861 attagatgta ttgggggcag tttttggtgt cggcttattg atttttgtga agatcccaaa
    19921 agtttctcct gaaatattgg aagttccgct tactattttt aaagatgcaa aatttgggct
    19981 gcagcaattg atggataata aagggttatg gtacataacg atcaatggtg catttgtgat
    20041 gctgttgttc atgccagcaa tcagtctgta tccattgatg acgctagatt actttggtgg
    20101 ttccgttgga caggcgggag cagtggaagt agtctacgca gtcggaatgc tcttaggcgg
    20161 tgcactgatc agttttatag gaacatggaa agaccgaatg aaacctatta ttatagccta
    20221 catcattatg ggcctgacga tcggtgcaag cggattggtt ccaaacgata gtcaagggtt
    20281 cttgtacttt cttatcttga atgcaggagc aggttgtgca acgccatatt tcaatacact
    20341 gctgatggca atgatccagc agagttatga atcgaatgtt ttaggtcgtg tcttagggaa
    20401 cttcaactct ttgatgaacc tggcaggtcc aattggatta ttatttgcag gaccattagc
    20461 tgatcgttta ggggtagaaa aaatgttctt gttttccgga atcgggattt tattatgcgg
    20521 aatcgtttta tttttaacgt cagctgcccg aaattatgat aaagaattac agaaaaaact
    20581 ggtaaaagaa caccatgagc aaaaagacga atagtatgga atggagttgt gacagaagtc
    20641 ttgttacaac tccatttctg atcctaatgt tcctgtggca ataagcctac tcaaatgaaa
    20701 aatatgagta attatcttta tttgtttaat acttgatata gggcagtttt ttcacgacct
    20761 atcgtacttc ctctttgctt catagatttc tttgatttgt tgctcagata acggagtgcc
    20821 aaatggtagt ttttccttta agagttctct ttccggtgcg tctacgccta cattgacatc
    20881 gttctcgata ggtacgctgt aatgatggat atgtccatga agattgcgga tagtgtcgtt
    20941 aggcccgagc aacatcggat aatgagtcaa gtaatattgt tgatggtcga atttaacgat
    21001 ggcacctaca tcataaaaat aaaatttggg acttccgtca gtaagcctag gatcattttt
    21061 ttctaaataa cggaaaaaag cgcgactgtc atggttccct ttgatcaagt ggattgttcc
    21121 ttgcagttga gtgaccagtt ccaaaatttc tgcatttgct ttttcatatt gcggatgcat
    21181 agcaaggtcg cctagatgat aaacatggtc ggtttctttt attactgcat tccagctagt
    21241 gatcaactgt tggttcattt catccacatt tgcaaagagc cgcggagcaa agtcgttttt
    21301 tcctaataag cgttcatgga aaaagtgcat atcactgata aagtaattca tgtatctttt
    21361 gcctcccttt tttcttagag taaacaaaat cacttatctg agcaactcat aacatctaaa
    21421 aagaaaaggt tgtaaatgag acgtgcagct ttaagcgata agaagcaatt ttgaaaaata
    21481 gtttttcaca atttataaaa ttattcctta ttgccgaaag gctggccctt tttatgagta
    21541 tgccaatttt ttatatcgaa tgctcaaaag atgagtgatt ttttggttga ctatcatgtt
    21601 tgtgtactga ataggtccag tagaacagta tttttacgac ttgataatta agggatgaaa
    21661 ttttcagaaa tatgacgtat aatgtaaaat actgaaaagt tgcgaccttc aactcgtaga
    21721 tgtttcagaa tctaattgaa atgatggtga tatcatgaca gacaacagca aaacacgcgt
    21781 tgtcgtaggg atgagcggcg gtgtcgactc atctgtaaca gcacttttgt taaaggaaca
    21841 aggatacgac gtgatcggaa tattcatgaa aaattgggat gacacggacg aaaacggtgt
    21901 atgcacggcc acggaagatt ataaggatgt agcgaaagta gctgctcaaa tcgggattcc
    21961 ttattactct gtgaattttg aaaaagaata ttgggatcgt gtatttgaat atttcctagc
    22021 cgaataccgc gcaggacgta cacctaaccc agacgtgatg tgcaacaaag aaatcaaatt
    22081 caaagcattc ttggattatg caatggatct tggcgccgat tatgtagcta ctggacatta
    22141 tgcgcaagtc actcgcgatg aaaacggcgt ggtccacatg cttcgtggag tagacaataa
    22201 taaagatcaa acttacttcc ttagccaatt atcacaagaa cagctttcaa aaacgatgtt
    22261 ccctctaggc ggcatggaaa aatcagaagt acgggcaatt gctgaacgcg caggccttgc
    22321 gactgctaag aaaaaagatt caacgggaat ctgctttatt ggtgaaaaga acttcaaaca
    22381 atttttgagc aactatctgc cagcaaaaaa aggaaatatg gtcactcttg atggtgaagt
    22441 caaaggacaa catgctggct tgatatatta tacgattggt caacgtcaag gactagggat
    22501 cggtggcgga ggagattcgc aagaaccctg gtttgttgta ggaaaagatc tagcgacaaa
    22561 cacattatat gtggggcaag ggttccatca tcctgcattg tatgccacaa gcttggatgc
    22621 aagtgaaatc cactttacga cgaatgaacc aatgccaaaa gaattcaagt gtacagcgaa
    22681 attccgttat cgtcagcaag acgttcctgt gacagttcgc ttattggatg ataatcgtgc
    22741 agaagtcgta ttcgacgaac ctgtacgtgc aatcactcct ggacaagcag ttgtatttta
    22801 tgatggaatg gaatgtctgg gtggtggttt gatcgaccac gcatatcaag aaacaaaagt
    22861 tttacaatac gtttaagcga tattaaatga atcaaaacag ctatggctaa caaaagaaac
    22921 accttttctt ttggatagca tagctgtttt tttgcaataa aattaatttt tattcatttt
    22981 atacttgaaa tacttgtgta aaaagttcat aatatccaat aatatgaata aagttgcttt
    23041 ttgttaagca agagcagttg caaaatagaa gggaatcttt tgcaagtgat ttgttcaatt
    23101 ttataagtga gggtgatgga aatggaaaag aaatcgacgg gtattagcaa atggggactg
    23161 gttgctctgg ttgtcagttc gtcaatcgga agcggtgtgt ttgggatcac aagtgatctg
    23221 gctagttctg cagctccagg gccagcgatc ctttcatgga taatcgttgg gattggtatt
    23281 ttagctttgg tattatcgct gaatcattta ggagaaaagc gtccagatct agatggtggt
    23341 attttcggtt acgcggaagc ttcatttggt aaattaggtg gcttcatcag tggttggggt
    23401 tactggttgt ctgcgtggct gggaaatgtt gcttttgcaa cgatgctgat gagcgccatg
    23461 ggagaatttt tcccgatatt caaaggtgga caaaatgttc cttctatttt atttgcaagt
    23521 atttttattt ggggtctgac tttattagta aacaatggag ttgaaggagc gagcgtcatc
    23581 aatacaatcg tgacgatctg taaattagtg ccattattcc tatttttaat atttgtattt
    23641 atcgcattta aagtgaatat attcactgct gatttttggg gtaacgtatc tgataatcta
    23701 gtgaatggaa cagggcagca aggaacgatc tggttgcaga ttaaaggttg tttgatggtt
    23761 atgatgtggg tctttgtagg gatcgaaggc gcaagtgttt tagccaaccg cgcgaagaaa
    23821 cgttcagaag cccagcaagc atcgatcatt ggtctgcttt gtttattgtt tatctatatc
    23881 cttgcttcga tcttgccata cggcgtctta tctcaagaag agttagcaac tatttcacaa
    23941 cctgctatgg caaatatttt aaaaggaatc gtgggaaatt ggggagcagc gctgatcaat
    24001 atcggcttga ttatttcgat cattggttct tggttgtctt ggacgatgct cccagcagaa
    24061 acaacgatgt tgatggctcg agacggtctg ttgccgaaag gttggggaaa aacgaataaa
    24121 aaaaatgccc caacttattc gttagttgtt actgcaatct tgactaatct attcttattg
    24181 actttcttag ttactgatta tgcctatcaa tttgcctatt ctttatgtac agcggcaatt
    24241 ttgatttgtt atctacttgt cgggatctat caagtccagt tttctttaaa agaaggaatc
    24301 aaaaaacaag tagtgatcgg tgggattgct gtagtttttg aattgatggg catcatttta
    24361 gcaggatttt cttatgtcct tttatgtagt attgcctatc ttcctggttt ttgcttctac
    24421 tggatcgctt gtcgtgaaac caaacatcaa attacgggaa aagaaaaagt aatgattggg
    24481 ctgatttgtg tggccgcact gctttcaatc gggttgcttg ctgtaggctg gatcaagata
    24541 taagggggaa agaaacatga aaatcgcaaa tttcggtgta gaagaatggc taaataaatg
    24601 ggaaaagaaa gcggtatatg atattgcaca aagctcgatc gaagcattga cattagacga
    24661 actcgttggc ttagatggga ctagcgtaag taaatttttc gaaaaacgca aaaacgaacc
    24721 attggactat ggctggatcg aaggatcaga tgttttcaaa caagaagtcg cttctcttta
    24781 tcaaaccgtc gatccggaaa acatcttaca aacgaatgga gcgactggtg ctaatctttt
    24841 agctttatac gcgttagtgg aagcaggaga ccatgttgtt tcgatgcttc ctacttatca
    24901 gcaattatat gacatcccca aatctttagg tgccgctgtt gactttgttc atttgaagga
    24961 agaagaagat tggcagtttg atttagaaca gctagaagcc ttagtcaaac ctgaaacaaa
    25021 aatcatctgt ttgaatagtg caaacaatcc tacaggcaca ttgctagatc ggaaaacttt
    25081 agaaaaaatc gcagaaattg ctcgtacagt agatgcatat gtattgatag atgaagtata
    25141 tgcgccttta acggataaag gtgaattctt gtcgatcgta gatgtctatg aaaaaggtat
    25201 agcaaccaat tccctatcca aaacctactc cattccaggt attcgtattg gctggacagc
    25261 tactggacct gagttggcag aagtttttcg aaagtatcga gactacacga tgatttgcgg
    25321 cggagtcctg tcagatgatc ttgctgtaca tgcattgaag aataaagaaa aaatattggc
    25381 gcgaaacaaa aagatcatta cagagaacct tgtgatttta aaacaatggg tagccaatga
    25441 accgaaagtt gaacttgtcg ctcccaatta cgtctctact tctttcatta aattagccat
    25501 cgaagaagat gaccagacat tttgtatcaa tttattagaa gaaacaggag tactattagt
    25561 accaggaagt gcctttgatt taccgaagca tgcccgacta ggttactgtt gtaaaaaaga
    25621 aaccttggaa aagggattat tcctcttatc tgagttcttg aaaaaacagg cctaaacaaa
    25681 aacactgcaa ggacagtctg tgacaactgt tcaagcagtg tttttcattt acgcttattg
    25741 tctatgagaa ggagagtgcc ctgaagctat ggagactcct gaagcttttt cttctgcagt
    25801 ttcttgctga tggatctctt gtgtattgtg tcgtacaagc aagaagttga ccaaaacaag
    25861 aagagaagtc gaaagaaaga cgccacgata accaaatgca ctggaaacac tggagccgat
    25921 cataggccct actacgttac cagttgcttg gaaagattgg ttataactga agatccgtcc
    25981 agcagcttga tgaggcgaat attttgtgat caatgcttgg acagcaggga gcagacaagc
    26041 atcagagata ccaatcaaaa aacgtaaggc agctaattgc caaacattcg tgacgaatgc
    26101 cattgggata taaaccagaa tcgcaaaacc caaaccaatc gcaagtattc tttcactccc
    26161 aatcttgtca cctaagcgac caaatcttgg agcggctatc aaagtggcaa tcccaggtat
    26221 cgaagcaatg attccgctga tcaacgtaac attcccatgt ccatgcagca attggcgaat
    26281 gtacagactg atgattggac taatggaatt gttggatgct tggatgatca tcgttgtgac
    26341 gaacataccg atgacgacgt gtggatattt caagtctttg aaaatctgtt tggcagaaac
    26401 catgttttct tttttcactg ggacaaattt ttcatggacg aaaaacagac tcaacaagaa
    26461 gacgaaaaat aagatgatcc ctgtaatgaa aaaggtaggg cgatagccaa acgctgaagc
    26521 agagatcccg ccaagaagcg gtccaagtaa tgttcctgta acagaaccag ttgctaaggt
    26581 tccaagaact tgaccacttt tttcttttgg cgtcccagtt gcgacaaggg cagtggcatt
    26641 actgatataa ccagaaaaaa tcccttgaag cagacgcaat gcgactaatt gatacacact
    26701 agtcacgcaa cccatcaaag aaatgacgat tgccattcct aatgaagcac gcaacagcat
    26761 caattttctt cctttttggt cggctaattt tccccaccaa ggagagataa gcgtagtcac
    26821 caagaaagta gatgaaaaag tcaatccgct ccaaaagttc aattgtgacg tactataatt
    26881 acctaacgta tctatgtaaa gtgacataaa aggcatgact aaactaaatc cgataccagc
    26941 cataaatgtt ccaaaccata agacaaacaa gttttgttcc caagtttctc ttttcctaaa
    27001 taatttttcc tttattcttg cagccaatta gactccttct ttctgaaaat tttcatctac
    27061 agctctttat tataaactac aatatgtagt ttatcaatga aagtctcttt tttctctttc
    27121 tttttcgtgg taaactgaaa gagtgaagga tttattttat gagaggaaac atggaaatga
    27181 aaaaaagaaa tctgtctcaa gagaaaatca tcgactgctt tcgagaacta gcagaagaga
    27241 tggatgttca gcaaataacc tttcagcatt tagccaaacg cttggatatc aaggctccct
    27301 cgctgtataa ccatttcaaa aatattcgtg atgtcaaaac agcgctaaca gcgaaacttc
    27361 tgcaggaact taatgaccag ctgcgacggg ctcttgtggg aaaaagtggt caggaagcac
    27421 tgcagatcta tgcagaaact tatcgatcct ttgctttttc aaatcaagca gtgtatgagt
    27481 tactgatcag tgtaccgcat accaatgaag ctgttttatt agatggaatc catgaaacga
    27541 atcagatcat tttgcagata tttgaggctt ttcaattaac gaaagaagaa aaagttcatc
    27601 gaagcaggga acttcgtagt atgatccacg gttatctttc cttacgtttt ttaggatact
    27661 ttacaaaaga accaacaatc gatccagaag acagctattt ttggatgatc aataacttta
    27721 tcgctacttt gccttaaaca atcagattca tttgtaacaa ttttaagatg ttcattaaat
    27781 gaccctttta taagagaaaa ttcttgcaac tttcttgaaa agtgactaag atgagtctag
    27841 aaaagctgaa agaaggagca cgagtatgta tcaagtaatt accatgttcg gggacaatga
    27901 gccttggtgg ttttttgaag actgggaaga agatattgaa gaagaagaaa cattcgagtc
    27961 tttcgaagaa gcacgacaag cgtacgaaaa acaatggaac gaaatccatc aaaattatga
    28021 atatatcaat gcaaaaagta actttctcag cgccttttgg aatgacggag atgagcgctg
    28081 gtgtgaagag tgtgacgatg atttacagca atacaaggga cttgcgttgt tgaaaaatca
    28141 tcagcccatc acagtagaaa gcagaaagga attttatgaa acaactaatt ctagcggaaa
    28201 aaccaagcgt tgcgaaagac ctaagcaagg tgcttggtgc taatcaaaag cacaaaaatt
    28261 attatgaagg acccaaggtg atcgttactt gggcccttgg tcatttatta ggattgaaaa
    28321 tgccggaaga tctgaataag gaatggcaga gctggcaaat ggacacgttg ccaatgatcc
    28381 ctaaaaaact aggaatcaaa ccacttccta aaacaggaca tcaattaaaa gcaatcaagc
    28441 agctggctca gcgaaaagat gtctcagaag cagtcattgc gacagatgcc ggaagagaag
    28501 gggaacttgt tgctcgctgg atcttagagt atgtgcattt caataaacca gtgaagcggt
    28561 tatggatctc ttctcaaaca gataaagcaa tcaaaactgg attcaagcaa ctaagacctg
    28621 cgaaagaata tgatgcattg tatgaatctg ctcttgcccg ggcaaaagcg gactggctgg
    28681 taggattgaa tgtgaccaga gccttaactg tgaagtacgc ggataattta tcagcaggcc
    28741 gtgtgcagac accgacacta gcattagtga gagaccaaga aaagaaaatc gagcagttcc
    28801 gtccacaaac gtatttcacg atcacgttgc aagctggaaa agaacaagcc aaaatgcttc
    28861 aaaaaaatca atttgcttta aaaacacaag aagaagcaca aaaacttgtt caattgatca
    28921 gtacaagcaa aggaactgtc acagaagtcc aagaaaaatt aaaaacggaa tctgcgccat
    28981 tgccttatga tttaacagag atccaaagag aagccaatca acgctatggc tattctgcaa
    29041 agaaaacatt aagtcttgta caaagtttgt atgagaccca taagatcgtt acttatccgc
    29101 gtacagacag caaatatctg acaaatgaca tgaaaagtac gatgaaagaa cgattgcaag
    29161 cagtcagcga ctttgctcct gaggtcaagc agtatttgaa aaacggagcc atcgtccaac
    29221 agacaaaagt attccaagac agtaaagtga ccgatcacca tgcacttttg ccaactgaaa
    29281 acagagcacg ttatgaaaaa ttaagtaatg aagagcgaaa aatctatcaa atgattgtct
    29341 ctcgtttttt aggactgttt gctcaacccc ataaagtcag ccaaacaaaa gttacggttg
    29401 aatttggtaa agaacgattc atttttcggc aaaatcgtgt cgtccaagca ggatggaaag
    29461 gcgaaactga aactgagacg gaaacagtca aatgggaaaa aggaatgaga atcaatcccg
    29521 atttcgccat caaaaaagaa ctatctgcac cgcctaaacc attgtcagaa gcaagcttgc
    29581 ttggtcaaat ggaaaaatac agtctgggaa ccccagctac gcgtgcagag attatcgaga
    29641 aactaatcaa atctgaactg atggaacgta cccctcaagg actgcaagta tctcctaaag
    29701 gtaagcaatt attagtattg gtaaatccct ctttagtaac accagatttg acagaaagct
    29761 gggaaaaaga ccttgaagct attgctgcag gaaagaaaca agcaggaacc ttcttgaatg
    29821 gaatcgaaaa agaaacaaaa cgcttggtga atgagatcaa atcaagcaaa caagaatatc
    29881 aagatttctc gattacccaa aagaaatgcc cagaatgtgg ggccaatctg cgcgagaaaa
    29941 acacacgtga cggcaaaatc tatgtctgca ccaattcaga ttgcaactac cgcagaagaa
    30001 aagatccaaa agtctctaat catcgatgcc cgcaatgtca ccggaaaatg gaaatcatcg
    30061 aaggtaaaaa cggtgcttat ttccgttgta aatacgatgg aacgacagaa aaattgcttg
    30121 ataaaaaaga gcagaagaag aaaatgacca aacatgaaga acgacgcttg ctgaaaaaat
    30181 atgcacaagc agaagaacca gaagaaagcc cattagcagc agcactaaaa gcagcaatga
    30241 agaagtaata agatatgaaa gaggttgtga tagaagtggt cagcttcaag aaataagaag
    30301 gaatttacga aaattgcttc tcaaattttt gtgaaatttc ggcttatttc cgaagaagct
    30361 acttctggaa caccatttat tcggaaaaaa ggactgtgac aagacttttg tcacagtcct
    30421 ttttcattta aaaaaatgtt tacttcattt gacgtatatt attggtgagg cttcaattca
    30481 tttggcttca ctttttctgt ttctcttatg atataaacag gacgctgttt cgtttctaag
    30541 aagattttcc caatatattt cccgatgatt ccaagacata acaattggat tccaccaatg
    30601 aacaggaaga tcgagaccat ggaaggccat cctgatgtag gatcaccaaa caacaatgcc
    30661 cgcaaaataa tgaacaaaat tgcaatacca gaaccgacac aagacaaagc gccgataaat
    30721 gaagcaatcg ttaacggtgc atccgagaag ttcacgatac catcaatcga gtagttgaat
    30781 aatttccaga aggaccaaga tgtttcgcca gccacacgtt cgcggttttc ataagaaata
    30841 tattctgttt taaatccgac ccagctaaat aatccttttg aaaatcggtt gtattctgtt
    30901 aattctagga cggcatccac catttgacga gtcatcaagc ggaaatcacg agcgccatcg
    30961 accatttctg tatcgctgat tttattgatc aactggtaga attttttcgc aaagaaactg
    31021 cgtatcgcag gctcgccttc acgagtcgaa cgccgggtgc cgacacaatc gatatcagaa
    31081 gtagtgatcc ggttgatcat ctcaggaagc agttcgggcg gatcttgcag atcaacatcc
    31141 atcacggtca ctaaatctcc tgtagaagct tgcaatccag cgtacagagc cgcttctttc
    31201 ccaaaatttc tggaaaatga gacaaagcgg acataaggat aagctgaggc tagcttcttg
    31261 attgctgcta acgtaccgtc ttttgatccg tcgtcaataa acaaatattc gaactcatgg
    31321 gcacctttga atttttcaac ctcatcaaaa aaaagaggaa cagttttttc ttcattaaaa
    31381 catgggacaa caattgatac tttcatcgtt tctatccttt caatcgtatg tttatatatg
    31441 gtttacattc aaaatttaat aaaccttaaa taacaccttt aaaatttgat tttacgtaaa
    31501 agaacaggta ttctttttgt aaactaacgt atttagttta ccatgtttcc tcggtttatc
    31561 aaacttttaa gaacaatttt tattagttgg caagtagttt tggatagaga aaaaggagga
    31621 agttccagtc tattgactag gagcaaacaa aatgaaaata cacacaccta tcggcttttt
    31681 atggttagat gcagatgaat ccggattgac tgctgtatct tttcagccaa tttcagaaag
    31741 cgaatcagcg aatcagcgaa ttttaacgcg cacacaagaa cagtttgaag aatattttgc
    31801 gggaaaaaga aaagaatttg atgttccttt atctataaag agaggtacgc cttttcagca
    31861 aaatgtctgg aaagctttgt cgaccattcc ttatggagaa atccgaacgt ataaagaagt
    31921 agccgttgct gttgatagtc cgaaagctgt gcgagcgatt gggcaggcaa atcgaaccaa
    31981 tccattgcca atcgttattc cttgtcatcg agtgatcggg caaaatggaa aactgacagg
    32041 atatatggga aacgcaatag atggggttga aatcaaacgc caattgttgt tgttagaagg
    32101 atatctgcaa aattaaaaac agttgtgatt cggacattgt tccatgcgag aatcacaact
    32161 gttttttcat gttcattcta aactttgatc agccaaataa atagtaaagc catcactgtt
    32221 tggagaagcc cgacagctaa tccataagat aagaagcgtg taccttcttt catgaatttt
    32281 gcaaagtcta aacgcagacc aattgccgct aaagcagtga tctcaaacca gctgctgata
    32341 aaatgtgccg attggtcaat aactacaggt aaagaaaaca agctgttgac caaacaaaag
    32401 atcacaaacc ctaatacata ccaaggaagc ggtaattttc gttttttctg cgtatcagtt
    32461 ggcgtttcaa acagagctgg ttgtttcttg tgcatgaatt tttcaaacag atagacaaca
    32521 acaacgagca tcataatacg tagaatctta aatagcatgg cgaattcgac aacagaattg
    32581 tcaagtaagc ttgccccagc gactacttga ccaacagatt ggagtgtacc acccaaaagt
    32641 gcactttttt caatgagatt tccaccaaac aactgtaatc caagaaaagg gagagtcaac
    32701 atcatcacag tacccagtag attgactagt gtgatgatct gtcctttttc ttcctctttc
    32761 gcatggatgg aaggggcgat tgaagcaata gcagaagaac cacaaacagc gtttcctcca
    32821 gccatcatca aagaagaagt atcgctgaat tgcagttttt taccaatcaa gtaagcaccg
    32881 atgatagtga ctgacatttg cagtaacaca aaaacaattc cttttacacc aatttgagca
    32941 atcgtttgag ctgtgacagt tgcacctagt aaaaaaacag agaattctaa tagtttgctt
    33001 tcagcaaatt ttgtgccgat tgctagaaca ggctgtttaa aaaaagtatt tcctaataaa
    33061 atacccaata aaatagcaat agtcgctcca cctaactgtg gcagataaat agcaagaagc
    33121 ctgctgaagg cagcgatggc aaaagaaagc agtaaaccgg gtaagatttc gcggaccgct
    33181 gaatccttta cagattttat tggatgtgac aatgatttca tttgatcggt tcctttcgtc
    33241 aataaatact aaaagcatac tcgaacatag tagcactaaa ccagtatttt gacaaaagaa
    33301 caagaggtcg tgacaaaagt cttgtacgac ctcttgtttc gaataaacgg tgttccagaa
    33361 gtagcttcta cggaaataag ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat
    33421 tccttcttat ttcttgaagc tgaccacttc ttttacgacc tcttggtaaa cggtgttcaa
    33481 aagcttgcat ctgcggtaat aagcccaacc agtacaaaaa catgaacagc tgtttttgtt
    33541 tggccgttct tattacttga tgcagaacag ctttttcaca acctattgaa tcgattatta
    33601 agcgattttt ttagaaacaa attttccatg tttgctatgt tctttaatta agaaacgtac
    33661 gtaatgagca agaacgtaga agacaactac acttgcaaac agacattccg caacttttaa
    33721 gaaatccaat acgctactat agatacctaa actgttgaag taatgaacag tagtaaatat
    33781 agctgttgca gagataacaa gcgggaatgt aaaggctgca tagcttggat aaaaacgaag
    33841 cttgaacaaa cgtggaagca tagacagcac aacgaaatac aatacttgag ataaagcgaa
    33901 caatgctacg acaagataaa aattaggttc agcaaagttt ttaagataac ctgttaggca
    33961 aagagaacca ggtgctgcaa cgatcgtaat cagcggacgt gtcggatgag ccatatttct
    34021 tacgaaaaag acacgtttga tgatgatcgg caataagaac gcatagcaga tcaatgaaat
    34081 ccaaaagaaa atttttccaa taagtgggaa gaagttcccg gcagttacag tgatgacccc
    34141 aaatccaacg taaatgataa accagcttgg ataaattgct tcaattgaaa gatcagcttt
    34201 gataacatgg tgataattga aataaaagac aaggatcact tggaagataa cagcgcacag
    34261 ccaaatgaat ttgacaattg gtgcaatact ttcatatgaa acaaaataag tacaaataac
    34321 catgagtgac atcgtaaaag ttggtgaaac ggaggcgatg attggatcat gcaacgtttg
    34381 tttcgtgtgt tcgaataaga taaacaattt aacaaaaatg aatatcataa gaatcataga
    34441 gataccgcca gttagattgc ctaaagcaac atggtgatag tctttaaaca gatttccaag
    34501 ggaagctaac ccaaggatca acccgcagat cgggatcgga ataattttca aaaagttctt
    34561 catatataat attctccttt ttatttaata gattttagta ccaagatgaa cgaatacatt
    34621 gtaaaatgaa taggaatgta tgattacgag caagcagaca gctgattgtc ttcatctaag
    34681 ttactagttg gatcagcaac tgttttgttg tacatttcta ttatagatgg ttttttgtat
    34741 atttaaatca aatagttttt atagtaatta tcataaaaag ttatggaagt ggtgccatgt
    34801 ttaagttatt aacgactttt cgagcagtat atgaaacaag aaatttttcc agagcagcag
    34861 aagtactgtt tttatctcaa ccagcagtct cgaatcaaat caaacagttg gagcgggaat
    34921 tgaatatcga tctttttcaa cgtaacggaa gaaaagagat gcttccaact aagcaagccg
    34981 acttattata tgaacggaca ctggttttgt tagaagaatg ggaagaaact cagcaaaaat
    35041 tgctcaatga acaaaatgaa gaagaagtat gtcggatcgt tgcttcccac acctttgctg
    35101 tctatctttt gcctcagttg atgaaacatc tgatcaaaag gtttcctaaa gtgcattttg
    35161 agatcgaaat cacgaattct catgaatcat tagaacaagt agcgaaacat gaatccgatt
    35221 ttggttttat cgaaaagcca ttagagactt ccggcgtaca aagatactct ttgatggaag
    35281 accagctagt catcgctggg gacccaaaaa gcaaattgtg gcttgtaaga gaagcaaact
    35341 ctggtgtttt tcattacacg cagcgttatt ttgaagagat gaatatccaa gataagaaga
    35401 tgatcgtgaa aagtaacgat gtaattgttg cgttgctgaa ggaaggaatc ggtcgctcga
    35461 ttttgtctaa gcgctctgtt ccagaaaatg tgccaatgac tccgttgaat ttccatcgat
    35521 atttttattt catccaacga accaatctgg tttctgatca gctgctagag attgccgaag
    35581 agatccttcg atacaatgaa agcacagaaa cttctcacag cgaggagaat ctcagctggt
    35641 agataattgc atgtatcaaa aaaagttcta tgatacacta aagataagca gtaggctttt
    35701 gtactgcatt tgctcaagca actgcacaaa cggaaagtta atttttccca aatgtctatg
    35761 gaaagacttt ccattatcta actggaccgc tataataatc agcagtatgg tttttaagaa
    35821 aagggatgaa acatggaaaa aaaattaaaa gcttcgaacg ataatcgtat tgattatggc
    35881 gtgattttac cagtctttct gttatgtttg attggtctgc tctcattata tgtagcactt
    35941 agccatgatc cgaacaatcc agcagtcttg aaaggcgtag ggatgcaagt tgtctggtat
    36001 ttagttggag cggcagcaat cgttgtcatc atgcatttga attccaaatg gatctggaaa
    36061 ctgactcctt acctttatgg tttggggtta gtggtaatgg gactcttgct caaattctat
    36121 gatcagaatt tagctgcttc aacagggtct aaaaactggt ttcgattcgg tgcgtttact
    36181 ttccagccag cagagttgat gaagattgcg tacatcttga tgatggcact gatcgtaact
    36241 aaacataata cgcaagtaaa agaacggacg atgaaatcgg acttttggct gattgggaag
    36301 ctcttgatcg tgacaatacc ggttttagca ttgatcatgg cccaagatga ctttggaacg
    36361 atgcttgtct ttcttgcgat ctttggcggg attttcctga tgtctggaat ctcttggcgc
    36421 atcatcgcac ctgtagtaat cttggcagta gtcgttggag caggaacgat cttcttggtt
    36481 acgactgaag gcggaagaga cttgctttat aaagttggat tcaaatctta tcagtttgca
    36541 cgtattgatt catggctgga ccctttccat gatacgtcag gaatgagtta tcagccggcc
    36601 caaggacttt tggcaattgg taccggtggc ttgtttggta aaggattcaa cgtaagcaat
    36661 atctatgtgc ctgttcgtga gtccgatatg atttttaccg ttatcggtga aaacttcggc
    36721 tttatcggtg gtgcattcgt cattttcttg tattttattt taatctatcg tatgattcgg
    36781 gtgtgtttcg atacgaataa tgaattttac gcctatatcg caagcggttt gatcatgatg
    36841 ctgctgttcc atgtctttga gaatattgga gcaaatatcg gattacttcc tttaacaggg
    36901 atcccattgc ctttcattag tcagggagga tcctcgattt tagggaacat gatcggtatc
    36961 gggttgatat tatcgatgag ataccaaaat gaagcacctg caagatcttc aaggaggtaa
    37021 gaaaattgta caccttttac tggtacccga aatgttcaac atgcaaaaaa gccaaagcgt
    37081 ggttagacca gcatggcgtt agttatcaaa ccgttgatat gatcaaagaa gtaccgaagg
    37141 cctcaacgat tgagacttgg ctagaaacga gtcaagtacc aatccgtcgc ctgttcaaca
    37201 caagcgggat gaaatatcgg gaacttggac tgaaagatca agtaggcgag tttacggctt
    37261 caaaagcgag ccaagtctta tcgacagatg gtatgctgat caagcgacca ctgatcttac
    37321 gagaggacca gcttgttgca attggattca atgaaaaaat ttatgaagga gaactgatat
    37381 aaaatggcag gcaagcattt gaagaaaaag gacaaccttt ggatcttatt taatggaaaa
    37441 gaatactgtg ttggtttgac aaatgaagcg caagaagaac ttggagacat cacgtttgcc
    37501 aatctgccaa aagcaggaca atcgctaaaa aaagatgaac ctctgatcga agttgaagcg
    37561 gaaaaagcgg tcaatgaatt tgccagcccg ctaacgggta cagtttcttc tgttaatgat
    37621 aaaatcggtg aagatatcaa tatcttgaat gattcagatg aaatgaatgc gtggatctta
    37681 agtttcaaag atgttgatcc aaaagaattt gacgaattat aaaatcagaa aatcacttga
    37741 aaaaagttga cactgattct tccagttgct ataatcataa gcaatcctat atgaaacgag
    37801 aaagctttga aaagaagagt acatgtcacg gacgtttgaa gagagccctg tttgctgaaa
    37861 atgggtaacg aaaatgagat ggaagatggt cttagagcag agtaagcgag cgtaagcaaa
    37921 cttactacgg gagtgcccgt tacagcgcga cagtatacgg aaagaagcta agtccaagta
    37981 ctggaagagg ctgttattgc gaagtaacgg taaatcaagg tggtaacacg gaagttcaag
    38041 ctttcgtcct tttgcttaag gcaagagggc gaaggctttt ttgtttgaaa aatcataaga
    38101 gaaagatgtg aatggcatgt cattaatcca actaacgaat gtcaaaaaga cattcaatgg
    38161 gaaaaacgga aacatccaag ccgtcaatga cgtttcacta aacgttgaaa aaggggatat
    38221 ttatggaatc gtcggctatt ctggcgcagg aaaaagtaca ttagtccgtc tcctgaatgg
    38281 acttgagctt cccacgagcg gcgaagtgat cgtaaacgat caagatatta caaaattaaa
    38341 aaacaaagaa ttacgtacat tccgaaagaa aatcggtatg atcttccagc atttcaacct
    38401 gttatggtca agaactgtac ttgaaaatat ccagctgccg ctggaattag ctggcgtacc
    38461 aaagagcaaa cgtaaagaac gggcagaaga actgttgcgt ttagtcgggt tagaaggacg
    38521 cggacaagca tatccttcac aactttccgg cggacagaaa caacgcgtcg gtatcgcacg
    38581 agcattggca aatgatcctg agatcctgct ttgtgatgag gctactagcg cattggaccc
    38641 gcaaacaaca gaagaagtat tagacttatt gttagcgatc aataagaaat tgaatttgac
    38701 catcgtcttg atcacacatg aaatgaatgt gatccgcaaa atctgcaaca aagtcgctgt
    38761 gatggagttg gggaaagtcg tagaagaagg agacgtattg actgtcttca gacaaccgaa
    38821 aaaagaagta accaaacgct ttgtacaaca agacatcgaa ccagaagctg attcgacaga
    38881 actattagct gaactcatca aagaaaatcc aagcggccgt ttggcaactt tgacctttag
    38941 cgaaagaaat gccaacgagc cagtcatttc ccaagcgatt cgacgctata acatcgatgt
    39001 caacgttgtg tatggaaaga tcaaacaagt gaaagaaggt tcctttggtt cattgactgt
    39061 cttgatgact ggtgaaacca aagaattaga tcaagcagaa gtgttcatca aagaacaagg
    39121 agtaggaatt gaggtgatcc atcgtggata aaagcttttc agaaacatat ctagatttta
    39181 aacaaatcaa cccggaaacg atgaatcagg ctatccttga tacactatat atgacattaa
    39241 tctcaatggt cttagtgacg atcatcggat tagttttggg attgatcctt tactctattg
    39301 gacgcaaaaa caaaccgtat gctcgaatca tttatggaat cgtatcgatc atcagtaata
    39361 ttttccggtc aacgccattt atgatcctga tggttttgat cattccattt actaaagcaa
    39421 tcgtaggcac tatgctaggt gcgcaagcag ccattccagc tcttgtttta tccgcagcac
    39481 cattttatgc ccgtctagta gagattgctt ttcgtgaagt cagccctggt gtactggaag
    39541 cagcagatgc aatgggagca agttactggg aaatcatctg gaaggtcttg atacctgaaa
    39601 gtattcccgc attgatttct ggattaacag ttacaacgat ctcgatgatc ggatttactg
    39661 cgatggccgg cgcgatcggc gcaggtggac ttggaggtct tgcttggcag gaaggctacc
    39721 agcgtggtaa tctaacagtg acattcgttg cgactttaat tattttacta atcgtattta
    39781 tcgttcaagg aatcggtgat ttcttaacta agaaaacaga caaaagataa taggatatag
    39841 ggggatgtag gatatgaaaa agaaaatttt tggatttgca gcagcgcttt tgctgactgt
    39901 aggattagca gcatgcggta acaacagcga cagcaaagat tcagcaaata aagcagacga
    39961 cacgacattg aaagtcggcg cttcaccgac accgcacgca gagattttgg aacatgtgaa
    40021 accattattg aaagatgaag gaatcgatct tgaaatcgtc aaattcgatg actatgtatt
    40081 accgaaccaa tctttggaag aaggcgacat tgatgccaat tacttccagc atattcctta
    40141 tttgaacaaa gaaatcaaag aaaaaggata tgactttgtc aatgccggtg ctgtccacat
    40201 cgaaccaatg ggcttgtact ctaaaaaagt caaagatgtt tccgaattaa aagatggtgc
    40261 aacaatcatt acttcaaatt ctgaatcaga ctggggacgc atcatcacaa ttctacaaga
    40321 tgctggctta gtcaaagtca aagatggagt agatctggaa acagcaacat tcgatgatat
    40381 tgaagaaaac cctaagaact tgaagttcaa ccatacgatc gacccatcac ttttagcttc
    40441 tacgtaccaa aatgatgaag gtgacctagt tgcaatcaac gcaaacttcg cttacggcgc
    40501 agggttgaat ccattagaag atgccgtatt gcttgaaaag gacaactctc cttacgtgaa
    40561 tattattgcg actcgtaaag gtgatgaaga cagtgacaaa atcaaaaaac tgattgaagt
    40621 gcttcaccgc aaagaagtcc aagaatggat cacagacaaa tggggcggct ctgttaaacc
    40681 agtaaaggct gatgcaaaat aaggtatgga acaaaaaaga tgagaaataa gtctgtaatg
    40741 gcttatttct catctttttt atacctattc taatttagaa atcaaaattg tttaataata
    40801 aataaaagat atttgatttt agtaaggaag gtcattgcgc ttcattctca attagtctaa
    40861 aatgagtgag aacgaaagag aatttggggg tataatgcat gtctgtctta gaaataaaaa
    40921 atctacatgt gtcgatcgag gaaaaagaaa tccttaaagg cgtaaaccta acaatgaaaa
    40981 ccggagaaat ccatgccatc atgggaccta acggcacagg aaaatctacc ttatctgccg
    41041 ctatcatggg caatccaaac tatgaagtaa cagaaggcga aattttgttt gacgggaaaa
    41101 atgttttaga cttggaagta gatgaacgtg cgcgacttgg cttattttta gctatgcaat
    41161 atccaagtga gataccaggt atcacgaatg cggaattcat gcgtgcagcg atcaatgcga
    41221 aacgtgatga aaacgacaaa atttctgtta tggcattttt gaaaaagtta gatgaaaaaa
    41281 tggctttact aaatatgcca gaagaaatgg ctgaacgtta tctgaacgaa ggtttttctg
    41341 gcggagaaaa gaaacggaat gagatcctgc aattgttgat gttagaacct acatttgcaa
    41401 ttttagacga aatcgattca ggtctggata tcgatgcttt gaaagtcgtt gctaaaggag
    41461 taaatgaaat gcgcggagac aatttcggtg cattgatcat cacccactat caacgattat
    41521 tgaactatat cacacctgat gtcgttcaca tcatgatgga aggccgagta gtcatgacag
    41581 ggaatgctga cctagctaaa cgtctggaag cagaaggcta cgctggtatc agtaaagaat
    41641 taggaatcga ctataaagaa gaagaagcgt aagggggagc aagaatgaaa gaaaaaaact
    41701 ggctcgatta tcttgacgcg gtaaatgatt tttctttaag taaaggcgaa cctgattgga
    41761 tgagaacctt ccgtcaagac gcactagcga aagcagatga actgccattg ccgcatatcg
    41821 atcgggtgaa attccatcgc tggtcgttat ttgatgtgaa agaaacacaa accatcagtg
    41881 aaacaggcac gattccggca tttgatgcaa tgaaagacaa tcctgtgctt gtccaacaag
    41941 gttcatggac gatttttgaa caactgcctg tggaattagc agaaaaagga gtcattttta
    42001 ccgacttgtt cactgcaatg atcgaatatc cagaactagt ccaagaatat tatatgaaaa
    42061 aagcagtaaa catgaacgaa gaccaattga ctgcactcca tgtagctttt atgaacagcg
    42121 gtattttcct gtatgtgcct aaaaatgtcg tgatcgatga accactcgag tccttattca
    42181 ttcaagatgg cgcatccgat gagcacttct tcaagcatgt gttgatcgtg gcagatgaac
    42241 atagtgaatt ttcttatctg gaacgtttcc agacaaccaa agaacaagta gcaaaatcat
    42301 caggtaatat catcgtggaa gtcatcgcta aagctggttc aaaaatcaaa tattcagccg
    42361 tagatcaatt aggagaaaat attacttctt atatgaatcg ccgtgggcac attttacgag
    42421 atgcttctgt cgactgggca attggtgtca tgaatgacgg tcatgtgatc gctgactttg
    42481 actcagattt agcaggagaa ggtgcacacg cagaagtcaa aatcgtggca atcagctcgg
    42541 gcagacaaat ccaaggaatc gatacacgag taaccaataa agcccctcat acgatcgggc
    42601 atattttgca acacggagtg atccgagaaa aaggaacatt gacctttaat ggtatcggcc
    42661 acatcttgaa aggggcaaaa ggtgcagatg cccaacaaga aagtcgtgtc ttgatgcttt
    42721 ctgataaagc acgtggtgat gcgaatccta ttcttttgat cgatgaaaac gaagtaactg
    42781 ctggacatgc tgccagtgta ggacgagttg atccagaaga aatgtactat ctgatgagta
    42841 gaggattgca taaagaagaa gcagagcgct tagtgattcg aggattcctc ggttctgtct
    42901 taacagcaat ccctgtagaa caagttcgga aagaattagt cgaagtaatc gaagggaagt
    42961 tgaatggatg atcgatgcta aaaagctacg caatgatttt cctatcttat ttcaagacgt
    43021 caatgatgaa cctttagttt atctggataa tgcagcgacg acccaaaagc caaaacaagt
    43081 actccaggca ttagcgtact attacgaaca cgataacgcc aatgttcatc gcggtgtcca
    43141 tacgttagca gaacgcgcga cccatgatta tgaacaagca agagaaaaag tacgggcgtt
    43201 catccatgca aaagaaacag cagaagtgct gtttactcgt ggtacgacga caagcttgaa
    43261 ttgggtagcc agaaccttcg gcgaccgcta tatcaaagca ggagacgaga tagtgctctc
    43321 ttacatggaa catcattcta atgttatccc gtggcagcag ttggcaaaac gaacaggtgc
    43381 tgtcttgaaa tatatcgaac taacagaaga aggcttcctg gatatggagg atgcagcgaa
    43441 aaagattacg gacaaaacca aaatcgtctc cattgctcat gtatccaatg tcttaggtgt
    43501 cttcaatcct gttcaagaat tagctgaact tgtccatgaa aacggcgggg tcatcgtagt
    43561 agatggtgct caatctgccc cgcacatggc cattgatgtc caagcactag actgtgattt
    43621 ctttgctttc agcggacaca aaatgtgcgg accaacaggt attggtgttt tatatggtaa
    43681 acgaaaatgg ttagaagaaa tggaacctgt tgaatttggt ggcgaaatga tcgattttgt
    43741 cgaattatac gacagtacat ggaaagaact accttggaaa ttcgaagcag ggacaccaaa
    43801 tatcgccggc gctatcgcat taggccatgc aatcgattat ttgcaaaaac tcggtatgga
    43861 caatatccat cgatatgaag aagaactagc cgcttatgtg ttgccaaaat tacaagcaat
    43921 tgatggattg actgtttatg gtccacaaga cccgaatttt cggacaggag tcgttgcatt
    43981 caatttggat gggttacatc cccatgatgt cgcgacagct ttagacatgg aaggggtagc
    44041 cgtcagagca ggtcatcatt gtgcgcaacc gctattgaag taccttgaag tatcagcgac
    44101 tgctcgcgcg agcttttatt tctacaacac aacgcaagac gccgaccgtt tagttgaagc
    44161 aatcatggca acaaaggagt ttttccaaca tgggactatc taaattagac aatttatacc
    44221 gtcaagtgat cttggatcat tcttcccatc cgcatcatcg tggaagcttg actgactcga
    44281 caagcaagat cgaattgaac aaccccacat gtggagatgt gatcgaattg caagtcgatg
    44341 taaaagacgg aatcatcaaa aatatcgcct ttgacggcag tggctgctcc atcagtacgg
    44401 caagtgcctc gatgatgaca gacgccgtca tcggaaaatc aatagaagaa gcagaacatt
    44461 taactgtaga ctttctacag cttgtccaag gaaaagaagt caaagatccc gaccaattag
    44521 gcgatgctgc tatgcttggt ggtgtagcta aatttcccgc tcggatcaaa tgtgcgacac
    44581 ttgcctggaa agcactagga aaagcaatgg aaaacgatgg taccgcagct ttccatcatt
    44641 accacaagga agaggagtga gaacatgggc gtaccagaat tagaagaata taaattcggg
    44701 tttcacgatg atgtcgaacc ggttttcagt acaggagaag gcttgacaga agaagtgatc
    44761 cgtgagatgt caagaatcaa aggcgaacca gaatggatgc ttgaattccg tctgaaatct
    44821 ctagaaacat ttaacaaaat gccaatgcaa acatggggcc ccgacctttc agacatcgat
    44881 ttcgatgcca tcaaatatta ccaaaaacca agtgacaaac ccgctcgtga ctgggaagac
    44941 gtaccagaaa aaatcaaaga tacatttgaa aagattggta taccagaagc tgaacgagct
    45001 tatctagccg gtgcttccgc acaatatgaa tcagaagtcg tgtaccataa tatgaaagat
    45061 gaattcgaaa agttaggaat cgtctttact gacactgact ctgctttgaa agaataccct
    45121 gaattattca aagagtactt ttcaaaactt gtaccgccaa ccgacaacaa attagcagcg
    45181 ctgaattcag ccgtatggtc aggtgggacc tttatttacg tgccaaaagc cgtcaaagtc
    45241 gatgttccat tacaaacgta cttccggatc aatgcggaaa acacggggca attcgaacgt
    45301 acgctgatca ttgttgacga aggagcaagc atccactatg tcgaaggttg tacggcacct
    45361 acttattcaa gtaacagcct gcatgccgcc atcgtagaaa tctttacaag aaaagatgcg
    45421 tattgccgtt acacaacgat ccaaaactgg tcagacaatg tgtacaactt agtaaccaaa
    45481 cgagcaaaag catacgaagg cgcaaccgtt gaatggatcg acggaaacct aggtgcaaaa
    45541 acaacgatga aatacccaag tgtttactta gatggaaaag gtgcacgagg caccatgctc
    45601 tccatcgcct ttgcaggagc aaaccaaatc caagatacgg gtgcaaaaat gatccacaat
    45661 gcaccaaata catctagctc tatcgtctct aaatcaattt caaaagacgg cggagaagta
    45721 aactatcgag gacaagtaac ctttgccaaa aacagtgcgg gttcgatctc tcatatcgaa
    45781 tgtgacacga tcatcatgga cgaatggtcg aaatccgata cgatcccatt caatgagatc
    45841 cacaacagcc aagtctcttt agaacacgaa gccaaagtct caaaaatctc agaagaacaa
    45901 ctctactatc tgatgagccg aggcctgacc gaatctgaag caactgaaat gatcgtcatg
    45961 ggctttgttg aaccattcac caaagaactt ccaatggaat atgcagtcga actcaatcga
    46021 ttgatcagtt atgagatgga aggcagtgtt gggtaaacct gttataggag ctagaaccct
    46081 tgatatataa gggttctagc ttttttgttt tgcctgtata ctagaaatga ccagattttg
    46141 accagctctt ttactgatga gccatcatat cagcaaagtc ttgacctgtt ttcttaggtg
    46201 cattatttgt aatatgtgca tagatgtcta atgtcacttt actattttca tgccctaatc
    46261 gttgttgaac taccttgaca gattctcctg cttctaataa taaactagca tgtgtatgtc
    46321 taaacccatg aggtgtaata cgaggcaagt tatatttttt caaaatatat cttaaccaat
    46381 cattaacagt gtttggaatg tatagagtgt ttttaactgt tgtaaaaaca tgttgctctt
    46441 tagagctagt attgtatcca agttttaaat actcttcttt ttgctgtaat ttccagttgt
    46501 tgaggataga aagagtctca gtatctaaag agataactcg tctagaagct cttgttttcg
    46561 gagattgaat tataacttta ccaaattcat ctacagcaag agttttattg acatgtagtt
    46621 ctttattaaa cgtgtctatg tctttccact gtaaagcgag tacttcactc tttctcatac
    46681 ctgtgtatgc caataatctg aagaaggcgt attgtttcat gttaccaaaa tctttaaaag
    46741 catccagtaa attattaagc tcctcttttg agtaaaattt acgatttttc tcatactctt
    46801 tttttcgtgg aagaagtgtt ttttccatag gattattgtc aatatattgc attgcaaccc
    46861 cgtagcgtaa aatttgtgca gtctgttttc tcatataatc gtaactttca tatttactgt
    46921 gccattcatc taccactttt tgacaatatg ggacagagat attcgatagt ttaagcttac
    46981 caaatgctgg aagtatttga ttatttgcgt atcttactga gattgcgatt gttgaagctt
    47041 taacactggt acgctgatga gttatccatt cattatacag gtcttcaaat gtcatatcag
    47101 gtgctttctt tttaacaccg tataatattt gatgtctgta ctcgtcataa tctttttgag
    47161 cttcttgttt gctactaaaa cctcttctcg tagtatttat tttttcacca gttcgtggat
    47221 cggtggcaat gtgtccttta tacatccacg ccacttgacc gtttttcttt ttgtactttg
    47281 atatttttgc cattaaatca tctcctctta gtttacaagg agtacttacc actagatggt
    47341 atcacctccc ttcatttttt tcttagtgta ttttttccca attgatgaaa tcgtttgttc
    47401 tagcttgttt atattatcag taatttctct cacagcttct tcgggataag acatgtcttt
    47461 caattgaata ggtagccatt catctaccat tctttctagt agttcttcta tactgctagt
    47521 gaggaaaaga atgttattgt caaatttttc tacaatagca ttgatgaata attcttctat
    47581 ttttaagata ttgaaaagac taattttttc atttttaact ttattacaag tcttgttagc
    47641 aataaattct tgcatctctg gttctaattt ggaatatcta tccagccatg tatctaaatt
    47701 agagtgtact ggtgggaact gttgacgaag atgctggaac aggtttttat ctggaaatgt
    47761 tataccatct agtacaatgc tatctctata aagaataatt cccaaaatat attcttctac
    47821 attgccacaa actaactcct ctatactcat atttcccaaa tcagcaattc gggataatcg
    47881 ctctggtatt ggtaaatagg ttcccttctc ccattcgtag atagtttttt tgtcaattaa
    47941 ttgaccgttg ttaatcataa gaccaaatgc tggttgtgtt aattttttac tctttcttat
    48001 agcttttatt tttgttccga gtatcttttt tcgttctaaa taatttattg gtggacgttt
    48061 tatcatattg accacacctc ctaaaattag tatacctcaa aaaaggggtg ttgtaaataa
    48121 attttacaaa gatgttgaca
//
LOCUS       DACDIH010000015        47468 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDIH010000015 DACDIH010000000
VERSION     DACDIH010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.015"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(53..787)
                     /locus_tag="IV281_000873"
     CDS             complement(53..787)
                     /locus_tag="IV281_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8779906.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="IV281_000874"
     CDS             1114..1524
                     /locus_tag="IV281_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP8779907.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="IV281_000875"
     CDS             1517..2218
                     /locus_tag="IV281_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP8779908.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="IV281_000876"
     CDS             2218..3804
                     /locus_tag="IV281_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8779909.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="IV281_000877"
     CDS             3823..4044
                     /locus_tag="IV281_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP8779910.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="IV281_000878"
     CDS             4041..4802
                     /locus_tag="IV281_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP8779911.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="IV281_000879"
     CDS             5182..5691
                     /locus_tag="IV281_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8779912.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="IV281_000880"
     CDS             complement(5766..6305)
                     /locus_tag="IV281_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP8779913.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IV281_000881"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IV281_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP8779914.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7372..8169
                     /locus_tag="IV281_000882"
     CDS             7372..8169
                     /locus_tag="IV281_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP8779915.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8197..8832
                     /locus_tag="IV281_000883"
     CDS             8197..8832
                     /locus_tag="IV281_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP8779916.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="IV281_000884"
     CDS             8852..9625
                     /locus_tag="IV281_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8779917.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="IV281_000885"
     CDS             10033..10830
                     /locus_tag="IV281_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP8779918.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="IV281_000886"
     CDS             10808..11221
                     /locus_tag="IV281_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779919.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="IV281_000887"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="IV281_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8779920.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="IV281_000888"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="IV281_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP8779921.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="IV281_000889"
     CDS             15998..16558
                     /locus_tag="IV281_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP8779922.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IV281_000890"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IV281_000890"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP8779923.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="IV281_000891"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="IV281_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP8779924.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="IV281_000892"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="IV281_000892"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP8779925.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="IV281_000893"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="IV281_000893"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP8779926.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="IV281_000894"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="IV281_000894"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP8779927.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="IV281_000895"
     CDS             21905..22483
                     /locus_tag="IV281_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8779928.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="IV281_000896"
     CDS             22535..23017
                     /locus_tag="IV281_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP8779929.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="IV281_000897"
     CDS             23134..23508
                     /locus_tag="IV281_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779930.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(24186..25538)
                     /gene="brnQ"
                     /locus_tag="IV281_000898"
     CDS             complement(24186..25538)
                     /gene="brnQ"
                     /locus_tag="IV281_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8779931.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            25685..26275
                     /locus_tag="IV281_000899"
     CDS             25685..26275
                     /locus_tag="IV281_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8779932.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(26403..27752)
                     /locus_tag="IV281_000900"
     CDS             complement(26403..27752)
                     /locus_tag="IV281_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8779933.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(27920..28660)
                     /locus_tag="IV281_000901"
     CDS             complement(27920..28660)
                     /locus_tag="IV281_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8779934.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(28673..30265)
                     /locus_tag="IV281_000902"
     CDS             complement(28673..30265)
                     /locus_tag="IV281_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8779935.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(30833..31129)
                     /locus_tag="IV281_000903"
     CDS             complement(30833..31129)
                     /locus_tag="IV281_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779936.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            31305..32030
                     /locus_tag="IV281_000904"
     CDS             31305..32030
                     /locus_tag="IV281_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP8779937.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            32023..32865
                     /locus_tag="IV281_000905"
     CDS             32023..32865
                     /locus_tag="IV281_000905"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP8779938.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            32868..33389
                     /locus_tag="IV281_000906"
     CDS             32868..33389
                     /locus_tag="IV281_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP8779939.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(33642..34205)
                     /locus_tag="IV281_000907"
     CDS             complement(33642..34205)
                     /locus_tag="IV281_000907"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8779940.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            34456..34725
                     /gene="rpsO"
                     /locus_tag="IV281_000908"
     CDS             34456..34725
                     /gene="rpsO"
                     /locus_tag="IV281_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP8779941.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            34913..37039
                     /gene="pnp"
                     /locus_tag="IV281_000909"
     CDS             34913..37039
                     /gene="pnp"
                     /locus_tag="IV281_000909"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP8779942.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            37549..37815
                     /locus_tag="IV281_000910"
     CDS             37549..37815
                     /locus_tag="IV281_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8779943.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            37959..39950
                     /locus_tag="IV281_000911"
     CDS             37959..39950
                     /locus_tag="IV281_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP8779944.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            40304..41605
                     /locus_tag="IV281_000912"
     CDS             40304..41605
                     /locus_tag="IV281_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8779945.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(41735..43105)
                     /locus_tag="IV281_000913"
     CDS             complement(41735..43105)
                     /locus_tag="IV281_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP8779946.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVNPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(43287..43610)
                     /locus_tag="IV281_000914"
     CDS             complement(43287..43610)
                     /locus_tag="IV281_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP8779947.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(43935..44543)
                     /locus_tag="IV281_000915"
     CDS             complement(43935..44543)
                     /locus_tag="IV281_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP8779948.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            44873..45589
                     /locus_tag="IV281_000916"
     CDS             44873..45589
                     /locus_tag="IV281_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP8779949.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            45602..46297
                     /locus_tag="IV281_000917"
     CDS             45602..46297
                     /locus_tag="IV281_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP8779950.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            46381..47010
                     /gene="udk"
                     /locus_tag="IV281_000918"
     CDS             46381..47010
                     /gene="udk"
                     /locus_tag="IV281_000918"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP8779951.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT        15390 a         8788 c         9878 g        13412 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcacagcatg acagcattgg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ctagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttttt ttgcgaaaac tatttttata tcatatatat gtctaaaatc tatattataa
    23881 aagaaatgtt gtttgatcag cttctcttcg gtattagttc aaaacaaaac ggcaaagtat
    23941 atggagctac tttgccgttt tgtttctcta atactcaggt cagtttatag acgctcaata
    24001 aacggtgttc aaaagccagt ttctcggtat taagtcaaat ttatgcaaaa atgtgagaag
    24061 ccattttcaa aaaatatttc ttaatactcg aaactgagcg gctttttcac gacctctcaa
    24121 taaacagtgt tcaagagaca gcctatcggc aataaggctg aacgtctctt tcacaacctc
    24181 ttttatcaat ctgctacttc ttctttctta gcgaaactcc aaatcagtcc gataacaaaa
    24241 ccgataactg ctggtacgat ccaccccatc cccaatttga aaaacggcaa gtagctttcc
    24301 gcaacatata aaagattttg aacaataggt gtttgtttga tagtttctgg gcaagcgttc
    24361 aacccatcaa aaatcgaagc aatcaatgta aaataagtag tcattcgata aacagccgta
    24421 cgctgcttaa acaaaggacc aactaacact agtaaaatca gtgtcattgc taatggatag
    24481 atgaacatca aaacaggcaa cgaaaattga atgatattcg tcaagcctac gtttgccgct
    24541 aaacaaggaa ggatactagc aagtgcaaca aagaatgcgt aatttctttg cggaaacaaa
    24601 tctgtgaatg tttctgcaaa agctgtaatc aaaccaatac cggtcttcaa gcaagctaat
    24661 ataacgataa atgctaataa gatactgcca taagtaccta aataatgatc agcaatctga
    24721 gccagcgtga tcccgccatt ttcacttaga gcaaagctac ctaagctcat agcacctaaa
    24781 taagcgagca aagtataaat gattcccatc aatataatgc tgatcgcacc agatttgatc
    24841 gtgtctttag cgatttcagc aggttttgtt acacccatgt tttggattgt tgtgacaatg
    24901 atgatcccaa atgctaaagc agcaagcgca tctaatgtgt tatatccttg agtaaaccct
    24961 gtgaaaaaag catgctcttg ataatttggc tgaataggcg cttgatttac agaacctagt
    25021 ggattcaaaa aagcaaggag aagcaaagca cctaataaaa ccaaaaatgc aggattcagg
    25081 aactttccaa cataatctaa tatctttgtc ggttttcttg aaaaccacca agccagcaca
    25141 aaaaatacaa tactgaaaat tgctagatat agcgtctgtc tgctaggatc gataaatggt
    25201 gctaaaccaa tctcgaaaga tgtggttgca aggcgaggta aagcaaaaaa cggtccgata
    25261 actaagtaaa gcaagatggt aaaaaccatc gcatatgttt gatttatccg ttttgctaat
    25321 tcatatacac ctgagctttt cgatacacca atggcaatca cccctaagaa gggaagaccg
    25381 ataccggtaa ccaagaaacc tagattcgcc caaaaggtag cagcgcctgc ttcttgaccc
    25441 aaatgaacgg gaaagataag atttccagca ccaaaaaata gaccgaaaag catggagcct
    25501 ataaaaagat agtctttaaa tgataatttt tttgtcatac ataactcctt tacggttttt
    25561 ttaaaagtaa caaaatttcg aaaaatatgc aacaattttc agaaaatttc ctatataaaa
    25621 cgacggttat ttaataacgt aagtgataaa ataaaagcgc tgaatcatct gaggaggaat
    25681 catcgtgaca aagtcgagat tagaagcatt tactgatgga gtggtagcca ttgttttgag
    25741 agttcttgta ttagagatca agatccctaa tacatctagc tttcattctt tatgggtcat
    25801 ccgaaataca ttattagctt ataccatcag ctttatattt gttgcggtga tctgggtgaa
    25861 ccatcatcgt atatttcaga tggcagaacg aattaattac cgagtggtct ggtcgaatat
    25921 ctttttgctc ttttggctga ctttatgtcc ttctgttact agttgggtcg ggcgtaaccc
    25981 tgaagctttt tggcctgaga tggcttatgt cttagtatac acgatgtgga gtttttcttt
    26041 tggtatcttg atgaggcaaa tcatgaaagc aaataaacca gattcccatg tcgtgagagt
    26101 tttgtcaaag gaccgtcgaa gtcttatctc catgatgatt aacttatgct tgattgccgg
    26161 tgtctttttt gtaccgttga tcgggttatt cggacgtttt tttgtatctg gtatttggat
    26221 tttttcctat aaaaaagcag atgcctatta tcagcggttg tttccgggga aatagaaaag
    26281 aggctgggac agaagctttt aactccaaga aataagccga aatttcacaa aaatttaaga
    26341 agcaattttc gtaaattcct tcttatttct cgaagctgac cacttctgtc acaactactt
    26401 tcttacttct tacgttcata tttcatatga tagattagta atagaagcaa gaaccacaca
    26461 ggtgttagta ataaagcgat tctcgtatct tttcctagga acaagacgac acacacaaag
    26521 gccatgtaaa gaaataccag ataattacta aatgggtaca acggcatttt aaatgaatga
    26581 gcttttttgc cgaggatact tttacgatat ttcatgtgcg tgtagacaag catactccag
    26641 ataaacaaga aacaagtagt ggcaatactg cttactagtg tgaatacttc actaggcatc
    26701 acgtaattca agataacaga aatgaaaatg acgatagtag aaaatgtcaa agcattccct
    26761 ggcacatgat gtgttgttaa ctttttgaat tttttagggg cactgccttc ttgcgctaaa
    26821 gaacgaagca tacgtcctgt actatatatt gcactgttgc aggcagaagc tgctgcactt
    26881 aatacgacta aattgatcaa ggaagcagca atcgtgatcc caatttccga aaatacttct
    26941 acaaatggac tgttatctgg attcaagctg ttccatggat agattgccat aatgacaaaa
    27001 agagatccta agtaaaataa gatgatcctg ataggaatat tattgatagc ttccggcaac
    27061 actttttcag gattttctgt ttctccagcg actaatccaa ctaattctat cccagcaaaa
    27121 gcaaaggtta ccatttggaa agctaagata aatccttttg caccagttgg aaagaatccg
    27181 ccatgagacc atagattcgt gatactggct gtgccaatat ctgtcgtata gtgggtgagg
    27241 atcatataag ctcctacgag gataaaggca atgatcgcta caactttgat caatgcaaac
    27301 caaaactcta actcaccaaa caatgagaca gccaccatat tcaagcaaag aagaagacct
    27361 aatgcaatca gttcaggaag ccactgagga acaccaggcg cccagtagcg tacatacatt
    27421 ccgatggctg ttaaatcggc cattgcaatc gatacccagc agaaccaata cgtccagcca
    27481 gtgatgaaag ccgctttttt gccaagatat tctgcaacaa aatccagaaa agaatggtta
    27541 ttggtgtttg ataacagaag ttcgcctaac gcacgcataa tgaaaaaaca gatgcatcca
    27601 gtaataagat aggcgagcaa tatcgaaggg ccagctagct ggatagactt tccggaacct
    27661 aaaaacaacc ccgttccaat cgctccgcca atagaaatca gttggacatg tctatttttc
    27721 agtccacgct gcagttcttg ctcttcattc atttttaaat catcctaact aaaaaagttt
    27781 aattacttcc atattaaaag agttttctga aaaacacaac taagattcca aaattttcag
    27841 aaatttacct tttatgagta attcataaaa aagaactgcg gtaaacaccg cagctctttg
    27901 tcccaatcag ctattgatcc taagcattta aaatacgtga aaggaattct tttgtacgtt
    27961 cttctttcgg atggacaaag atatcttctg gtgtaccttc ttctgcaatc acacctttat
    28021 ccataaaaat gacacggtca gatacatctt ttgcaaattc catctcatgc gtcacgataa
    28081 gcattgtcaa gccagtatgt gctaagtctt tgatcgtatt aagcacttcg ccaaccattt
    28141 cagggtctaa cgccgaagta ggttcgtcga atagcaatac atctggattc attgataatg
    28201 cccgagcaat tgcgacacgc tgtttctgtc caccagataa ttgtgctggt ttggcttcga
    28261 tataacgatc cataccaact ttttccagat tttcgattgc tacttttttg gcagtttctt
    28321 tatctcgttt caggacagtt gtttgaccag acatacagtt ttccaagaca ttcatattgt
    28381 tgaacaggtt gaaggattgg aaaaccatac ctagattcgt tcgatattta ggtaaattat
    28441 atttaggagc taatacattt tctccattat aaatgatctc gccgcctgtt ggtttttcca
    28501 gtaaattgat acagcgcaag aaagtagatt taccagaacc ggaagaaccg atgatcgtca
    28561 caacttcacc tttattgact gttacgctga tatctttcaa tacttcattt tcaccgaagc
    28621 tttttcttag atgtttgatt tcaataattt tttcattcat gattcttcct ccttattcct
    28681 gcaaatttga attgtcgatt tcttcaagct tggtataagc tgaaggacca tcgattttct
    28741 tttctaccgc ccgcaggatt ctggtgatcg taaaggtcat gactaagtag atgatcccga
    28801 cgatcgtaaa tgtttggaag aattggaaat tcgcaccgga agcagagttt ccttggaaga
    28861 acaaatcagc tacgccgatt acacttaaaa ctgctgtatc tttgatattg ataacaaact
    28921 catttcctgt tgctggcaag atattccgta aaacttgtgg aataacaact tttctcatcg
    28981 tttggccatg ggtcatacca atggcttgcg cagcttcgaa ctgcccttca tctactgcaa
    29041 aaatcccccc acgaacgatc tctgtcatgt aagctccagt attgagagag acaataaaca
    29101 aagcggcaat cgttcggttc aatgaaatgc caaaagcgag tgccagaccg tagaagatga
    29161 ccattgcttg taccatcatc ggtgttccgc ggaatacttc gacatagata gaaagaagtg
    29221 catttccgat tttttggaag aagcgagtca gtgcatttcc tgattcaggt accgaacgga
    29281 agacaccaat cagtaatcca agtgtagtac cgacaactgt cccgatcaat gcgatgaaca
    29341 aggtaagtcc agcacctcgt aagaacatat tgccgtattg tttccaaatc gtagtgaaat
    29401 cagaaatgat gccaggtttt tcttcactat cagttgcagc aggctgatct ttgactgctt
    29461 ggtccataat tttggttcgt tcatcgttag agatgccaga aagaatctgg tttacttttt
    29521 ggatatccgg gtcgccttta cgcataccaa cagaaatctg aacatcttcc gggtttgtct
    29581 ggaagccatc tgcaccgctg aattcaagca tttccaagtc tttgttcaca ctagttgccg
    29641 ttacaccttc cggacgttcg ctgacataac catcgatcat tccagaagct aaagctgttc
    29701 tcattgctga gaaattatcc attgcttgtt gtttctgcac atctggtatc tgatcgatca
    29761 cagtatagtg gaatgtattt aactgagcag taatctttgc tccagaaaga tctgctaaac
    29821 tttttgcttt tgcatatttg ctatcttttt gaactactac tactaattgt gattcgtaat
    29881 aaggatcggt aaaatcaatt tcttttcgtc gttcagcagt agggctcatt ccggcgatga
    29941 tcgcgtcgat cttgcctgat tgaagggcag gagccagacc atcccatttc gtttggacga
    30001 tgactaattt tttccctaaa ccatctgcta cttttttagc gatttgaaca tcataaccgc
    30061 cggcgtaact attttggcct tgaattggca aggcattatt ctcatctgtt tgttgagacc
    30121 agttgaaggg tgcataccca gcctccatac ctacgcgaaa ctcatcgctc ggtgtttttt
    30181 catctgcatt tgccagagat cccacactga atagtgagaa gacaaaagca aatagtacgg
    30241 caactgaaac catatttttt ttcatttttt tctccctttc tgattatatg atacacgagc
    30301 ggtctagatg aaatgcggta aagctttatc tgaaaaaata aaaagcgacc gatttataga
    30361 tataaaaagg ccgctaaaat tccataaggt gataaacctc tcacaaaaca tagcgcaact
    30421 tagcatcact gctaagacag tccgcaagct cttaacttgc gtcccaacat acactccaag
    30481 caaaaaagtg cacatttcgg cgatcctccc tattcctgct tctccatgtt tgcttcactc
    30541 cacagggtta atgattctcg cgacctctac ctcattgatt gaggcatctc gtattcagtt
    30601 atttatacaa aaaaagttta cgatactcga gaggctttgt caatctataa ctgctgtttt
    30661 ttgaaaaaat actgataaat atagaaatct ttacttttac cgaaaaaaca atcaaaatgt
    30721 gaatcatcta tccattgcaa acgatagtat aattatacac aaaaatgtat gtaatacaat
    30781 aaaaaaagaa ctagaacaga aaatttcctg tgtccagttc catctcgatc atttaagctg
    30841 ccgctttctt tggctcacgt cctaggatcg cttgcaaaag aacaacgacg ccataaatgg
    30901 ctaatattag ataaatcaaa cttgagtatt gggtgattcc ccagccccat gttcgatata
    30961 cggctaatgt aatggctaaa acaattgcgg caatcacgtt gaataaagca aaagcccaaa
    31021 gatttccatt ttctttgcgt acaagataaa aaacagagaa ataaacgctt aacgctaacc
    31081 aggcgataca agccagaata acgccccaat aaataaagaa tggtaacata tcgattcacc
    31141 tcacttttat acagcagtat tgtaacacgt tttcattctt ttgtcagtga acattgtgat
    31201 aaattgttca aatgcccata acactttttt attcccattt ttctgacttt tcaacaataa
    31261 atttgttcga gtatgggcta taatggaaag tgaggtgaca aaacatgaaa caattaacag
    31321 tagacgaagc agcaaaagag atattagctt atcagcaaat tacatttttg accggtgcag
    31381 gggtatccac accttctgga gtgccagact atcgatcttt gtcaggaatc taccacggga
    31441 tggagcagcc ggaatacttg ctgagtcatc aagcaatggt gaatgaaccg gataaattct
    31501 atcaatttgt caaaaagatt tatcatcctg aagcaaaacc caatatcatt catttggaaa
    31561 tggcacatct agcgaaagaa aaaaatgttt gggttgtttc ccaaaacatc gacggtttgc
    31621 acgcaaaggc taaaagcccg caactcgtca attttcatgg aagtttgtat caatgtcatt
    31681 gtcgaaaatg tcaacaaacg gtagactgga aagaatatct tcacagcgac aaacatcaag
    31741 catgcggagg acaaatacgc ccagacatcg tgttatatgg tgaagggttt caagatggag
    31801 tcatggagca ggcggcttat gccgtcagcc aagcagagct gatcgtcatt gttggaacga
    31861 gttttcaagt tcatccgttt tgtgatctgg tacagtttcg ccagccgtca gccaaaatct
    31921 tagtaatcaa tcaaacgcct gtttatttag cggagcctta tacgtttatt caagaagacg
    31981 gagcggttgt tttcaaaaaa atacaagaat taggagcaga ttatgactga attcaaaacc
    32041 gaaaaagaaa aaatgattgc aggcgagctt tattttgcca atgatcctga gctggttgcc
    32101 gaccgtatgt ttgcaagaag tcaaagccaa atcatcaacc aagcagaatc agcggaactg
    32161 cgatcccaat tgttgaaaga gacttttggc cgtacaggga aaaaaatcta tatggagcca
    32221 gttatcaatt ttgattacgg atataatata tttgttgggg agaattttta tgcaaatttc
    32281 aattgtacat ttttagatgt cagcacgatt gaaatcggtg ataactgcat gttcgctccg
    32341 aatgtccagc tttacacggc gacgcatcca ctacatccag tgaaacgtaa cagcggtctg
    32401 gagtacgcaa aacccattaa aatcggtaat aatgtttggt taggcggagg agttatcgtc
    32461 acgccgggtg tcactctagg ggacaacgta gtagtcggcg caggttcggt tgtgaccaaa
    32521 tcttttcccg acaatgtagt gattgcagga aatcctgctc gtatcatcaa gacagtagag
    32581 gaagaactaa cagaagagtc tcttgaaaca ttacgtcata agatcgatat gatcgatcga
    32641 gagttgattg ctttattaga aaaaagaatg gatacggtaa ctaaaatagg acaagtgaaa
    32701 aaagaagaaa accaagcagt ttatgatgca aaaagagagc agcaagtatt ggataaagtt
    32761 gttagtctgc tgaaaaataa gggatataag gaaacaataa cagatacgta tgttgatttg
    32821 atgaaacact cgcgcgaata ccaaaacaaa atgaaggagg aataagtgtg atcgaaggag
    32881 agacacggcg aaaacaaatc atgcaaacct tggcaaatac agaaaaacca gtgagtgcta
    32941 gcaagtttgc gaaaactttt ggagtcagcc gtcagatcat tgtaggcgat attgctttac
    33001 tacgagctgc aggtaaaagt atcgtggcaa cagcaagagg ttatatcctc gaaacagaag
    33061 aaaccaatac tgggcacatc agtaaaatcg ctgtccagca tggaaaagat caaacagaag
    33121 aagaactgag actaatcgtt gaaaatggtg gtgaaatcat tgatgtaatt gtggaacatc
    33181 ccctttacgg tgagttagca ggaactttgc acatcaaaac gccacaagat atcgattcct
    33241 ttatgagacg ttatcaaaaa agtaatgcta ctttgttatc cgaattaaca ggcggcatcc
    33301 atctgcatac gatccgctac ccagaaaagc atatgctgaa aaacatcaaa caaaaactag
    33361 ctgaagcagg aatcctctac gaaggataac atcgtagctg ctacttttta tcctagaaac
    33421 tattctatca tctctgagat aaacacccat atggaagaaa taagaaaaaa taagaaaaga
    33481 tgcgtatcca atacacagaa ttttttggaa ttgcgatttc tagatgtcta tctaaatttc
    33541 gattagtgga gaaacataaa aaagggaagt gcagcttttg agcactcccc ttttttataa
    33601 agctttcttg tagcatagta tcgaaggaac ctgccaattc tctactcgat caccaatacg
    33661 ccttctttta gtgcaaatgg attttgatcg ttgatgtggt catagaacat cacaccgttg
    33721 atatggtcga tttcatgttg aacgacgatc gattcataat tcttcaaacg aatcttgtgt
    33781 ttttcaccat tcatatcata ataagacaca gtgattttgg catggcgaac aacatatcct
    33841 ggtacttctc gatcaacaga taaacagcct tcaccttccc ctaaacaagc atcttgaaca
    33901 gaatggctca aaattttagg attatacata acagtactta gagaaggtcc atctgcctct
    33961 gggtcaggac ttggtacgtg aacagcaatg attcttttag aaatatctaa ttgaggagca
    34021 gctaagccaa caccgcctcg taagtgtagt tcttcagctt tgattggatc ttggctgttt
    34081 tttaagaact caagcatctc ttttcctaaa gagatatctt cttcgcttaa tggaagagaa
    34141 acttcttttg caacttcgcg taaggtcgga tttccttcac gaatgatatc atccattgta
    34201 atcatggaat atgtacccct ttcttaaaag actgtgtttc aagtctattt tcgcattata
    34261 gtttaccaca aaaatggaaa agtagtagta agattgtttg aaaactacct tttcttaatg
    34321 attaactact tgattttcaa cccggtttca ggtaaagtat agctgtgtgc tctaggcaca
    34381 tgacctgcca cttggttaag cgaatcacca acgttttact cagtcgcagg ggaataatcg
    34441 aaagaggtga aagaaatggc aatttcaaaa gaacgcaaga atgaaatcat caacgaatac
    34501 gctcgccatg aaggggatac tggttctcca gaagtacaaa tcgctgtatt aactgaagaa
    34561 atcaaccact tgaatgaaca tgcacgcgtg cacaaaaaag accatcattc ataccgcggc
    34621 ctaatgaaaa aagttggtca ccgccgtaac ttgttagctt acttacgtaa aactgacgtt
    34681 caacgttacc gcgaattgat caaacgttta ggcttgcgtc gttaatttta gtcaaaaaga
    34741 gtgggattca catgaatctc acttttttta ctcgtatatc aattaagtaa aattccgtga
    34801 tcaagctaga tggactccta ctaaccaaat gaaagctgct caaaagacgg ctttcggcag
    34861 ttttcatttg tttagtagcg tcttgctttc acgagaagga aaaggagaag ttatgtcaga
    34921 aaaacaagta ttcaaaacga catggggcgg ccgtccctta caaatcgaag tcggtcagct
    34981 agcaaaacaa gcaaacggag ccgttttagt ccgttatgga gatacagtag tcttaagtgc
    35041 agctgtggcc tctaaagaag caaaagatac agactttttc ccattaacga tcaattatga
    35101 agaaaaaatg tatgcagcag ggaaaatccc tggaggtttc atcaaacgag aaggccgtcc
    35161 aagtacggaa gcaacattga ctgcccgttt gatcgaccgt ccaattcgcc cgatgtttgc
    35221 agaaggcttc cgtaatgaag tccaagtaac gaacatcgtg atgagtgtag aaacagactg
    35281 ctcgccagct atggcagcaa tgctaggttc ttctttagca ctatcaatct cagatatccc
    35341 atttgacggc ccaatcgcag gcgtagaagt tggtcgtgta aatggcgagt acgtcttgaa
    35401 tccaacagta gaacaagcag aacaaacaga tatcgaattg actgttgccg gaacgaaaca
    35461 agccatcaat atggttgaaa gcggcgcgaa agaagtttca gaagaagaca tgcttggtgc
    35521 attgctgttt ggttttgacg ctatcaaaga gttagttgct ttccaagaag aaatcgttca
    35581 agcagtaggg aaagaaaaaa tggaagtgac tttactgcaa gttgatgaag tattgaaaaa
    35641 agaaatcttt gatgcttctt acgctacaat gaaagcagca gtaatgactg aagaaaaact
    35701 tgctcgcgaa gataatatcg agcaggtgaa gatcgatatc cgcgaagctt atgctgagaa
    35761 atttgcaggt catgaagatg aagatcactt gttgaaagaa gttaaacaaa tcacagaaga
    35821 tcttgaaaaa gatgtcgtac gtgaattgat caccatcgat aagatccgtc ctgacgggcg
    35881 taaattggat gaaatccgtc cattgtcatc agaggtcagc ttattgccac gtgtacatgg
    35941 atcaggtttg ttcacacgag gacaaacaca agctttatca gcttgtacac tcgcacctct
    36001 aggagagcac caaatcattg atggtttagg ggtggaagtc agcaaacgat tcatccacca
    36061 ctacaatttc cctcaatttt ctgttggttc tacaggacgt gcaggctctc cagggcgtcg
    36121 ggaaatcgga catggcgcat taggtgaacg tgcgttagca caagtaatcc cttcagaaga
    36181 agaatttcct tatacgatcc gtttagtagc agaagtattg gaatcaaatg gttcttcttc
    36241 ccaagcaagt atctgtgctg gcacattagc attgatggat gcaggtgtac caatcaaagc
    36301 gccagtcgcc ggtattgcga tgggacttgt ttctgatggt gaaaactata cgatcctaac
    36361 tgatatccaa gggctggaag atcatcttgg cgatatggac tttaaagttg ccggtacaaa
    36421 agatggtatc actgctttac agatggatat caagattcaa ggaatcacag aacaaatcct
    36481 aactgaagca ttaactcaag caaaacaagc acgtatggaa atcttagaag aattgacttc
    36541 aacgattgct gctccacgtg aagaattaag ccaatatgca ccaaaaatcg aaatgatcca
    36601 gatcgaacct gctaaaatca aagatgttat cggtaaaggc ggcgatacga tcaacggaat
    36661 catcgatgaa actggtgtga aaatcgatat cgatcaagat ggaaaagtaa gtattgcttc
    36721 agctgatgca gaaatgatca aaaaagctat caagatcatc gaagatttga ccaaagaagt
    36781 caaagttggt gaagtttatc tagggaaagt tgtccgtatc gaaaaattcg gtgcattcgt
    36841 caacttgatc aaaggaaaag atggacttgt ccatatttcc caattagcaa acgatcgtgt
    36901 caataaagtc gaagatgttg ttaaactagg tgatgagatc cttgtgaaag taactgaaat
    36961 cgataaacaa ggacgcgtaa atctgtcaag aaaagcaatg ttgaacgaag atggttcaac
    37021 gaaaaatgaa gaaaaataat ttaattcata ggaataatgt acttgtgatc gtgaaggtga
    37081 cagggggaca ataattattc gaaaaaaggg gactgtgaca aaagccgtgt cacagtcctt
    37141 ttttttcgaa taaacggtgt tcaaaagctt gcatctgcgg taataagtcc agccaagtta
    37201 aaaacattag gagctgtttt tatttggctg ttcttattac ttgatgcaga acagcttttt
    37261 tacaacctct ccaataaacg atgttcaatc agctgtccct cgatattagc tcaaaaaacg
    37321 gcaaaatatg aggaactatt tttccgtttg tttctctaat actcaggtca aaccgctgat
    37381 ttcacaacct ctgattcacg gtgttccaga agcagcttct acggaaataa gctgaacact
    37441 tttgttacaa cttctttttt tctggagttt cttggcgcat atttttggag tccttctgaa
    37501 taaagcgtaa aataaaagtg agaaatatgg ataaaggagg gaaaggagat gctgaatttt
    37561 ttatggtcgt tatttatcgg cggtatatta ggtttgatag ctggcgcaat tttaggaaag
    37621 gaagtcccgg gaggcgtatc tggaaatatc attatcggtt ttttaggtag ttggctcggc
    37681 gaatttttac taggaccact gggaccggaa ctcggcggtt tttatttgat accagcatta
    37741 ttgggtgcaa ttttttgcct tgctctttat tcatttgtgg caagacgtat cagagaacgt
    37801 ccaaagaaag aataatttta ttcaaataag ttgacacttc agtgtgttac ttggataatt
    37861 agaagcaatt taggaaagcg ggtgtcatgg ataagaatat catctatcat tcaagcttac
    37921 ctaaaggata tccatataac aagaaatgag ggaaaaacgt ggcaacaacg cacaatacca
    37981 aaaagatatc aatgactggc ctgcttgtag cgatgggagt cgtctacgga gacatcggga
    38041 caagtccgct ttacgttatg aaatcgattg ttgaagataa tggcggattg tcaggaatca
    38101 atcctgattt tgttatcgga tcagtctcat tgattttttg gacattgact ctactgacaa
    38161 caatcaaata tgtcgtgatt gcattgaatg cagataacca tggtgaaggt ggaattttct
    38221 cactctacac acttgtccga aaaggtggga aatatttgat cattcctgca atgatcggag
    38281 gagctgcctt actagcagat ggggtattga cccctgctgt gacggtaacg actgcgattg
    38341 aaggcttgcg aggaattccg cagttttttg accggttcgg taataaccag aacgtgatcg
    38401 tcattatcac tttagttatt atttttgttt tattcatggt ccaacgcttt ggtacagata
    38461 ttgtcggaaa agcattcgga ccaatcatgt tcgcttggtt tactttttta ggcgtaatgg
    38521 gattaatgaa cttcagtcaa gactggacag ttatccgtgc actgaatcct tattatgcta
    38581 tccagctgct ttttagtgca gataacaaat taggcttgtt cattcttgga aatattttcc
    38641 tggctacgac aggtgcagag gcgctttatt ccgatcttgg acatgtcggc aaacgaaata
    38701 ttcgtctgag ctggccttac gtcaaaattt gtttggttat caattatttt gggcaggctg
    38761 cgtggttgtt gaatgtttat caaaaccctg aagcacagca aatcaagaac ctgaatccat
    38821 tcttccaaat gatgcctcaa agttggatcg tgatcggcgt tgcttttgca acagtcgctg
    38881 cagtcattgc ttcgcaagca ttgattacag gttcgtttac gctagtttca gaagcaatca
    38941 agttgaaact gctgcctcgg ttaaagatca tgtatcctgg gaacagtatc gggcaaatgt
    39001 atatcccagc agtcaatctg attttatgga tcgcctgttc tctagtggtt gtcacattcc
    39061 gaacgtctca tcatatggaa gctgcttacg gtctatcgat cactgtgacg atgttgatga
    39121 caacagcact gctttacttt tatcttatcc aaaatgggca ttctcgttgg cttgcatatt
    39181 tagttacctt tttcttcggt gcgatagaag tcgttttctt tatctcaagt gttgtcaaat
    39241 tcttccgagg cggttttgta gccgttctga ttggtttgat catcttagtc gtgatgttta
    39301 tttgggaaca agggaacatt attcgcgaat cgtcaaccga ggatgtgtct ttgagagatt
    39361 atatcccgca gcttagagaa ttacgtgatg atacttcttt accaatctat cagacaaatg
    39421 tggtcttctt ggttcctgat atggctgaag ataaagttgg ccgacagttc atctattcga
    39481 ttttagataa acggcctaaa agagcacgag tctattggtt tgttcatgtg gaagtgacgg
    39541 atgagccata tacgaaagaa tatcaagtag atatgatgga cactgatttt attgtccaag
    39601 tgaatctgtt cttaggtttc agggtccaac aggaaatcaa cgtatatgtt cgccaagtca
    39661 tccatgattt gatgaaacaa ggccgtttgc caaaacagcc acaaagatac tctctgacac
    39721 cagggcgaga agttggcgat ttccaatttg tcctgattga tgaagtagtt tccaatgtga
    39781 caactctagg aaaatggcaa cgtcaaatca tgcaggcaaa actggcaatc aaaaaaatcg
    39841 ctacctcgcc agaaacatgg tttgggctag agtacagcga agtaaaacat gaaactgttc
    39901 cgcttatcat tggaaatact cggaaaacat ggttgaaaga gcggttgtga aagtagcgct
    39961 ttgacctgaa ctaatgtcga atgtcagcta cttgaacacc gtttatcaga ggtcatgaaa
    40021 aagctgtact gcaacaagca gtaagaacga gcagataaaa acagcttctc atgtttttca
    40081 tctgtttggg cttaatgccg tagatgcaag cttttgaata ccgtttttat cagaggtcat
    40141 gaaaaagctg tttagcaaca agtaacgcaa tgattttgaa gtgataagga gatgtggctg
    40201 aaaaatgccg catctccttt tttattccta aatatgaata cgcattattt tcaatctttt
    40261 tgtagaaaaa gggtacaata gaaaaagaga aaggcgggag aatatggtta tcaatttcat
    40321 tattttattt ttgatgattg caattacttc attttttgta gcaagtgaat ttgctttagt
    40381 gaaaatcaga cgatcaagat tggaacaact agaaaaggaa aatgtaaaga acgccaagct
    40441 agctcttcat gttacacacc atttagataa ctatttatca gctagtcaat taggaattac
    40501 gctaaccggt ttgatcatcg ggtgggtcgg tgaaggatct gtcgcggctt tactagaacc
    40561 agtgatcggt aaactacctt ttagttcggc tattagcagt acaatctctg tggcgctggg
    40621 atttatttta gttacgtata tcgatgtggt tattggcgaa ctgcttccta agagctatag
    40681 tatcgtcaat acggagaagg tcgtcttgtt cgtagttaaa ccacttcatt atttttataa
    40741 agtaatgttt ccttttattt gggtattgaa tcattcagct gcagggctag ggaagctatt
    40801 aggtgtgcgt ctcgtatctg aaggagaaga gacattgtct caagaagagc tgacattagt
    40861 cgcattgaat tcttatgaaa aaggagagtt gaccaaagaa gaatattatt atttggaaaa
    40921 cgtgtttgaa tttgatgata ccttggcaaa agatatacaa gtagaccgta cgtccatgca
    40981 agtattcgaa gcttcagaaa ccgtcaaaga agcgataaaa gcttcgattg aacaaggaca
    41041 tactcgctat ccggttatct tagaatcaaa agacaatgtt ttaggttatg tcaccttgcc
    41101 agatttgatc aagcaatctt ataaagatga ccagctgaca gtagaacagt tgattgaaga
    41161 gccgatcgta acaactgaaa cgatccctat aaaaaaatta ttgacgatca tgcgaaaaaa
    41221 gggtaagcat attgctatct taaaagatga atacggaggg acaagcggct tagtgacgat
    41281 tgaagatatt ttagaagaaa tcgtcgggga aatccgagat gagacagatt tggacgaagc
    41341 attgatagcg gaacagtcag atggctctta tatcatctcc ggcaaactaa cattagatga
    41401 tttccagcgt tattttcatg tagagattcc agagtttgaa gaaacgaatt ttactacact
    41461 tgcgggcttt gcttctagcc gatataaaga aataaaagca ggaacaatca ttgagattgc
    41521 atcattccgt ttcacggtat tagaatacca gcatgcacat atcgattatt tcaaagtaga
    41581 gtccacggaa agaaaaacag aataaaagaa aaaaggctgt gacatttttt gtcacagcct
    41641 ttttgttatc taaaaaacct gttttcggta aacgaagaag atattttcaa taaaagtttt
    41701 catttttagc cgcaacgatt tcttttaatc aaggctatct tgcttctgca tgttctaatg
    41761 tgaaatgttc ctcttcaccc atgtgtttca attgtttcat cgaacgcata taagagtaaa
    41821 gcaacacggc aacagaaccg atccaagcta aaggagaagc tgctgctgct cctgggtatc
    41881 cccacattcc tgtaagaacg atagctgcaa aagaacgcat aagcaattcc atcataccgg
    41941 cgatcgtagg gatcttgctt tgtccaagcc cttgaagtgt gtagcgtaaa atgaacagga
    42001 ctgctaagat ccagtaaaga gacccattga tattgaagta ggtttgtgct aattcaaata
    42061 cacgtgtttc tgatgggtta acaaacaatt gaacaaagaa atgaccgaag aaaatgacga
    42121 tagcacctgc taagagacta aatccgacac tcatcagtaa agtttgacgt accccttgca
    42181 agattcggcc atattctttc gcaccatagt tttgtgcagt gaaagttgcc attgcaatac
    42241 caaatgacat catcggctga tttgcaaatt ggtcgatacg accagcagcg gcttgagcag
    42301 ctacgacatc tgtccctaga ctatttagtg ctgcttgtaa cacgatagca ccaacagcga
    42361 tgatcgaaga ttgaaacgcc attggcaaag cagcatttag gtgtacagca aactcttctt
    42421 tttcaaattt gaaatctttt ttgcgtaact gtaacaaagg catttttttc ttgatataca
    42481 ctagacacag tacgctggaa gaaacttgtg cgattactgt agcaatagct gcaccttcca
    42541 cacccatgcc aaaatagata ataaagatca aatcaagtac gacgttgatg atgaccgcaa
    42601 tgatcagaaa gaatagtggt gtgcggctgt caccaagtgc gcgaatgaca ttagaaagca
    42661 aattgaaact gactgatgca aagatcccta ataaaataat tgaaataaat gtttgtgctt
    42721 gatcaagtat ttcaggagga gtttgcatca attgaagcat cggacgaata aacaggagac
    42781 ttaggacagt taaaatcaca gtgacgacaa gactaatgac aacactagca gcaaaacttt
    42841 tcttcagacc ccgataatct tttgcaccgt aacgttgtgc ggtaataatg gctaaaccag
    42901 ctgttaaacc ttgggcaaag ccaatgatca aaaatgtcag gcttccagtt gcaccaaccg
    42961 ctgccagcgc atttttacct agcgtctgac ccacgatgat catatcgatc atattgtaga
    43021 actgctgaaa aacatttccg atcagtaaag gaacagtgaa catcaaaatc agtttagcgg
    43081 ggcttccttt tgttaaatcg cgcatctttt aaactcctta tatctttaaa atttagttag
    43141 tagtatatag cgtttatcct gagaatacca attagaaatc aatgataatt tatgaaactc
    43201 cataaaaaga agccaagaat tgagtcaaag aatcaacaac ttggcctggt ctgtaaaata
    43261 agggacatcc acgtttatct atcaccttaa tattgtctaa tctcacttgg taaaaggatc
    43321 gtttctctcg taccgtcatc ataggcgtat acttgagcag gaaattggtt cgaataggca
    43381 aacggcaaat caatatccat ttccacattg cttcctgctt gagaaaaatg catcgtcact
    43441 tttccacgat tttcgatcaa atcaaaggtt aatatatttt ctaatggaat gactccctta
    43501 agatctaggt caataataaa ccatatactg tcaatcaatt catctggcaa acttgatacg
    43561 acaccaactg aagcgtaacg gttgcggctg ctatcaaaac tttcaaacat ctgtttaacc
    43621 tccttcgaat caacgaatgt tttattttta taattaatcc taggataaac tagaaatact
    43681 tacttcaacg agggttcatt ctaataaaga atcaggaaaa atgcaacaat aattgaatac
    43741 tttttttcaa agtataaatt tttataaagg tacgataaaa gtatgcgagg aaaagcttgg
    43801 taaattagga tttttcgttt gtaaagccat ttacaaaagt ggaagcccca tatgaaaata
    43861 aacgattaga aataaaaaag gcgtgttaaa agctgctgac ccgccgcttc taacaccacc
    43921 tgattgtttc agtattattc ttctgttgaa ccgatcactt ctggttcgcc accttcacct
    43981 gtgctttctt cttcttcact ttgagcgact acagcagcga tttgctcttc actgtcggtc
    44041 acgaatttgt aatcgtcgtt ttttggaaga tcggctactg taatagaatc acctaattcc
    44101 aagtttgtca cgtcaacttc aattcgttca ggcaatttgt ctggtgtagc agatacgact
    44161 actgtataca agttttgagc taacactcca ccagatttag cacccactga ttcgccaaca
    44221 agaacgactt ctgtttcgac ttctgtttct tccgacatgt ttacagatag aaattctgca
    44281 tgttcgaaat gattggtaaa agtgttcgtt tggatcttgt gaaccaatgt attgactttt
    44341 tgtccatcga tgtccatcgt gatgactgtg ttcgctccat gttcacgcaa caatttgctt
    44401 agtgtcaaac catcgaatga gataggggta ctttctactt tatacccata aacaacagct
    44461 ggaactttcc cttcgtgacg aagtttgttt cttaatgaac ggggacgtac ttctctttta
    44521 cttacttcta atgatactga cataaccaaa attcctcctt aaaatgtgac tgtctgcttt
    44581 ggtaacggac agattttatt taagtgaatt ctaaaggcta atctcgtgca catcatcgaa
    44641 ggcagggcgc ttaaaaaagc gcaaaaggag agatgcgcgc atctcccctt agaaagtcag
    44701 gattcaactt ttctccacca ggtcattacg ctgtgtatga gaaatccaat tcactttact
    44761 atttaaccgt aacgtgaact gttaattaag tcaaggaata actatgatga tgtatttgca
    44821 agcaagaata ttcaaggtat aattaattag tagtaataag aaaggaaaca gcatgcgttt
    44881 agataaatta atagaagaaa aattagaaac gacacgcaag gaaatgaagc ggctgtttgc
    44941 tttgaagcaa gtgatgatag atggcacagt cgaatttcga caaaatcgaa atgtagatag
    45001 tttactgcat caaatcgaag tagcaggtga acacttagaa acaaatgagt cctattatct
    45061 tctcaataaa cctgaaaaag ctgttacagc tgtatcagat ccagaaaaga cgacagtaat
    45121 cgatctgatt gcaagtgccg atcgtttcca accattatat ccagtgggaa gactagatag
    45181 agatacaaca ggactattgc tgatcacttc gaatggacaa ttgggatatg aaatgctact
    45241 tccagaaaaa aaggtttcaa agacttatca agtagttgtg aacgaaaggg tgacaccggc
    45301 agacgtaaca gcatttgaaa atggaatcgt attcgctgat gggtatcaat gtaagccggc
    45361 tgatttaaaa atactcaaag cagacaagca ccaaagtacg gttttattga cgatccaaga
    45421 aggaaaattc catcaagtca aaaagatgtt cttagcccaa ggaaagaaag tgaccgcatt
    45481 gaaacggcta agcatgggac cattggaatt agatgaacag ctaggagtag gcgagtatcg
    45541 atcgttgaca ttagcagaga tgaaaaagtt aagtatttat tttaaatagg aggtcaaaaa
    45601 gatgaaatat aaaactttat tgtttgatgt agatgataca ttgttagatt ttcaattaac
    45661 tgaagccaat gctttaagtg ctttgtttgc agaacaagga atcacattga caccagaaat
    45721 cgaaaccagc tataaggctt tgaatcatca tttatggcag gagtttgaaa aaggcagact
    45781 atcacgagaa gaaataatag gcagtcgttt tgggttactt ttccagcaat ttgggaaaac
    45841 agtagatagt gatcaaatgg aaaaacgcta ccgccactac ttgaaccaag gtcatgatct
    45901 tgttccagga agtctagaaa tcctgaaaaa ggtccatccg catgctgatc tttatattgt
    45961 gacgaacggt gtgtcgcaca cgcaacatcg gcgattaaca gatgcaggaa tgcttgatta
    46021 cttcaaggat atctttgtct cagaagctgt cggagcgcaa aagccgatga aagaattttt
    46081 tgatcatgtc ttttcccaca ttcctaattt tgataaaaag caaactgtga tcattggtga
    46141 ttctttgact tctgatatca aaggaggaaa tatggcaggg atcgatacga tttggttcaa
    46201 tagaaataaa ttaccagaaa tcgaagaaat ccagcctact tatcgaatcg attcattaga
    46261 ggaactttat tcattattgg aaattgaaaa ggattaattt tcagaaaaat atagcatttt
    46321 cgtgtttttt cgctataata gattagaatg aaaaaacgga tacgaaagag gttttagtca
    46381 atgacaaaaa gcaagccaat tatcattggt gtgacgggtg gatccggaag cggaaagaca
    46441 agtgtcagcc gtgcgatttt caacaacttc ccggaccatt ccatcatgat gcttgaacaa
    46501 gattcttatt acaaggatca gagccatttg agttttgaag aacgattgaa cacaaattac
    46561 gatcacccat ttgcctttga taatgatctg ctgattcagc atgtaggcga cttactgaac
    46621 tataaagcca ttgaaaaacc cgtgtatgac tatgtagcgc atacaagaag tcaggcaacg
    46681 atcattcagg aaccaaaaga agtgatcatt ctagaaggaa tcctgatttt agaagacgaa
    46741 cgtctgcgtg acttgatgga tatcaaagta tacgtagata cagatgatga cattcgtatc
    46801 attcgtcgta tcaaacggga tatggaagaa cggggacgca cattggactc cgtgatcgaa
    46861 caatatttga cagttgttaa accgatgtat catcaattta tcgaaccaac gaagcgctat
    46921 gcggatatca ttgttccaga aggcggagaa aaccacgttg ctattgattt gattacaaca
    46981 aaagtagcta gtttcttaaa ccataaatag tgcgcagcga aacagttgaa cggactgcga
    47041 ccgtttgaaa agcaaggatt ctgctttttg aacagtcacc agccaactgt ttttttattt
    47101 ataacaagag gttgtgaaaa cagcatcttg acctaagtat taaaaagaag aaacggaaaa
    47161 tagctgttta tattttaccg ttttttgagc taataccgaa ggtcagctga ttgagcaccg
    47221 tgaagtagag gttgtaaaaa agctgtcttt gcatcaagcg ataagagcat ccaaataaaa
    47281 cagctcctca cgtttttcgt tgggttgggc ttattatcgt agatgcaagc ttttgaacac
    47341 cgtttaacga gaggttgtaa cagaagtggt cagatgcaat aaataagaag aaacttacga
    47401 aaattatttc tcgaatttgt gtgagatttc gacttatttt tttgaagaag ctcttttgtc
    47461 gcaccccc
//
LOCUS       DACDIH010000016        45252 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDIH010000016 DACDIH010000000
VERSION     DACDIH010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.016"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(626..988)
                     /locus_tag="IV281_000919"
     CDS             complement(626..988)
                     /locus_tag="IV281_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779952.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(1062..1442)
                     /locus_tag="IV281_000920"
     CDS             complement(1062..1442)
                     /locus_tag="IV281_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP8779953.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1524..2699
                     /locus_tag="IV281_000921"
     CDS             1524..2699
                     /locus_tag="IV281_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8779954.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2776..3360)
                     /locus_tag="IV281_000922"
     CDS             complement(2776..3360)
                     /locus_tag="IV281_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8779955.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3561..4559
                     /locus_tag="IV281_000923"
     CDS             3561..4559
                     /locus_tag="IV281_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8779956.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4671..5180
                     /locus_tag="IV281_000924"
     CDS             4671..5180
                     /locus_tag="IV281_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP8779957.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(5210..5869)
                     /locus_tag="IV281_000925"
     CDS             complement(5210..5869)
                     /locus_tag="IV281_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP8779958.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5871..6635)
                     /locus_tag="IV281_000926"
     CDS             complement(5871..6635)
                     /locus_tag="IV281_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP8779959.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6643..7320)
                     /locus_tag="IV281_000927"
     CDS             complement(6643..7320)
                     /locus_tag="IV281_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP8779960.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7357..8742)
                     /locus_tag="IV281_000928"
     CDS             complement(7357..8742)
                     /locus_tag="IV281_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP8779961.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8952..9036)
                     /gene="ffs"
                     /locus_tag="IV281_000929"
     ncRNA           complement(8952..9036)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IV281_000929"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(9059..9574)
                     /gene="tadA"
                     /locus_tag="IV281_000930"
     CDS             complement(9059..9574)
                     /gene="tadA"
                     /locus_tag="IV281_000930"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP8779962.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9681..10538
                     /locus_tag="IV281_000931"
     CDS             9681..10538
                     /locus_tag="IV281_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP8779963.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10619..10954)
                     /locus_tag="IV281_000932"
     CDS             complement(10619..10954)
                     /locus_tag="IV281_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779964.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(11021..12391)
                     /locus_tag="IV281_000933"
     CDS             complement(11021..12391)
                     /locus_tag="IV281_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP8779965.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12469..13005)
                     /locus_tag="IV281_000934"
     CDS             complement(12469..13005)
                     /locus_tag="IV281_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8779966.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13314..14795
                     /locus_tag="IV281_000935"
     CDS             13314..14795
                     /locus_tag="IV281_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8779967.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14898..15605)
                     /locus_tag="IV281_000936"
     CDS             complement(14898..15605)
                     /locus_tag="IV281_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP8779968.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15684..16022)
                     /locus_tag="IV281_000937"
     CDS             complement(15684..16022)
                     /locus_tag="IV281_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP8779969.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(16133..17095)
                     /locus_tag="IV281_000938"
     CDS             complement(16133..17095)
                     /locus_tag="IV281_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP8779970.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKINVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(17110..18441)
                     /locus_tag="IV281_000939"
     CDS             complement(17110..18441)
                     /locus_tag="IV281_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8779971.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18449..19129)
                     /locus_tag="IV281_000940"
     CDS             complement(18449..19129)
                     /locus_tag="IV281_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP8779972.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19348..19827)
                     /locus_tag="IV281_000941"
     CDS             complement(19348..19827)
                     /locus_tag="IV281_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP8779973.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19954..21633)
                     /locus_tag="IV281_000942"
     CDS             complement(19954..21633)
                     /locus_tag="IV281_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8779974.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            22181..23683
                     /locus_tag="IV281_000943"
     CDS             22181..23683
                     /locus_tag="IV281_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8779975.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23696..24358
                     /locus_tag="IV281_000944"
     CDS             23696..24358
                     /locus_tag="IV281_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8779976.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(24459..24662)
                     /locus_tag="IV281_000945"
     CDS             complement(24459..24662)
                     /locus_tag="IV281_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8779977.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24773..25957)
                     /gene="iadA"
                     /locus_tag="IV281_000946"
     CDS             complement(24773..25957)
                     /gene="iadA"
                     /locus_tag="IV281_000946"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP8779978.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(26114..27853)
                     /gene="yfcC"
                     /locus_tag="IV281_000947"
     CDS             complement(26114..27853)
                     /gene="yfcC"
                     /locus_tag="IV281_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP8779979.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(28625..29239)
                     /locus_tag="IV281_000948"
     CDS             complement(28625..29239)
                     /locus_tag="IV281_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8779980.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(29845..30222)
                     /locus_tag="IV281_000949"
     CDS             complement(29845..30222)
                     /locus_tag="IV281_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779981.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(30522..31403)
                     /gene="galU"
                     /locus_tag="IV281_000950"
     CDS             complement(30522..31403)
                     /gene="galU"
                     /locus_tag="IV281_000950"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8779982.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(31446..32465)
                     /locus_tag="IV281_000951"
     CDS             complement(31446..32465)
                     /locus_tag="IV281_000951"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8779983.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(33098..34102)
                     /locus_tag="IV281_000952"
     CDS             complement(33098..34102)
                     /locus_tag="IV281_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8779984.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(34115..35164)
                     /locus_tag="IV281_000953"
     CDS             complement(34115..35164)
                     /locus_tag="IV281_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8779985.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(35181..36002)
                     /locus_tag="IV281_000954"
     CDS             complement(35181..36002)
                     /locus_tag="IV281_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8779986.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(35989..36768)
                     /locus_tag="IV281_000955"
     CDS             complement(35989..36768)
                     /locus_tag="IV281_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8779987.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(36785..37255)
                     /locus_tag="IV281_000956"
     CDS             complement(36785..37255)
                     /locus_tag="IV281_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8779988.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(37267..37686)
                     /locus_tag="IV281_000957"
     CDS             complement(37267..37686)
                     /locus_tag="IV281_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP8779989.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(37767..40535)
                     /locus_tag="IV281_000958"
     CDS             complement(37767..40535)
                     /locus_tag="IV281_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8779990.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            40735..40959
                     /locus_tag="IV281_000959"
     CDS             40735..40959
                     /locus_tag="IV281_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779991.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            41738..43453
                     /locus_tag="IV281_000960"
     CDS             41738..43453
                     /locus_tag="IV281_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8779992.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            43456..45219
                     /locus_tag="IV281_000961"
     CDS             43456..45219
                     /locus_tag="IV281_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8779993.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT        13421 a         9352 c         7642 g        14837 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcggg agtgtttccc gattcgtttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa gcgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt ttaacgactc
      301 agttatctta tcatgtcgtt agtaacatgt caataactat tttttaaatt ttttagaaat
      361 gtgatataac atccacttca gaatcaacgc tgttaaattt atcatgtatt tcttccttgg
      421 tcaactataa attaagcatt actttgtgaa atgatttttc gatcaattat tattattcgt
      481 tatacaaccg ttttcaacat attttgaaaa taaaatgcac taaaaaacta tctttagcag
      541 aacatttcat tgtatttttt ggaccaaaca ataaaaaaag aagtttgaaa aaaattcaaa
      601 cttctttgtt cgcttaatct gtattttaga agaaaggcac tccctctact tcgtaagttc
      661 caagaacaga gcgcatttga cctggttctt cccaaatata tccttcgcct aattcttctc
      721 tttgattctt ttgttcagtt tcaaagacaa acaacggaac attttcctct ttaatatgtg
      781 tatttgtcaa ttccatcaaa ctaatcttgc taacatcgat atgaactatt tcttttaaga
      841 aattaagtat attcgctaaa ccagtctttc cttcagaaag ttccgctaac gcaaattcca
      901 acttgtcatc aattgaatgg accaaaaatt ttttagtacc atcctccaaa tgcaacataa
      961 ttgtaccagc aactcttttg cttgccaaaa tttccacctc gatcaacttg ttctatggct
     1021 tacataaaaa ttctagcaca ttttcagaaa aaatacactt tttatttctt attttctttc
     1081 cgttctagtt cagataatgc ttcttctatc caaataggta actgctccgc aatagtggta
     1141 aatccgatct tacgattttt attcgtgaaa tacctttttc tgcgatatgg aacagctgga
     1201 tttgtttttt ctaacgttct taaagataaa ctgatttttt tggaatattc atcaatgtcg
     1261 atgatctgaa ctgttactgg ctgtccgact tttaacaatg aatggatatt tttcgtataa
     1321 ccggattgta cttctgaaac atgaataagt ccttgcgtct cgttatcaag agaaacgaat
     1381 gcgccataag gctgtatccc tgtcactttt ccttcaatta tttgtccaat tttataagtc
     1441 atctacttcc ctctcttcgc atttatcatt atactgtaaa tagctcaaaa aaagaagcga
     1501 cgaaaatgac tggaggtctt aagatgatcg aatttgataa acagatttca agaaaagata
     1561 cagaaagtgt aaaatgggac gcaattgctg agacatatca aatggatgac ttacttcctt
     1621 tgtgggtagc agatatggac ttcttgtctc ccggtggggt agctaaagcg tttagtgaat
     1681 acatcaaaca tggaatcttt ggttatgcca ctctgtctga taaactatat caggctatca
     1741 ttcattggga acgccaacac catgctgtgg agttgacgaa agaaaatatc gtttttacaa
     1801 gtggtgtgtt aaccagttta gctgcagcag tacaaacttt tacaaaacca ggcgattcta
     1861 ttttgattca tgacccagtt tatcctccat tttcctctat tatagaaaca aatcaacgtc
     1921 acatcgttcg aagctctttg ctagaaaaaa ataatcattt catcatggac ttgtctgaca
     1981 tggagaaaaa aatcgaagaa aacaatgtaa aagcaatgat tttatgcaat cctcacaatc
     2041 ctggagggcg tgtttggagc aaagaagaat tgaaacagtt aagcgaattg tgtttaaaac
     2101 atcaagtgat tctgtttagt gacgagatcc atcaagattt aactttattc gatcatacat
     2161 ttacttccat gctaacgatt gatcctaatt tagatgatct actagttgct tttacttccg
     2221 caaccaagac tttcaatcta gctgcaatca aaaattcaat ggtatttatc aaaaatccag
     2281 agatgaaagc tgattttgaa aaacatttag tgatgaacca acagcacgaa atcaacactt
     2341 ttggactgat tggaacccaa gcggcttatg agacgggtgg agaatggtta agccaactga
     2401 ttccttatct agaaaaaaat gttcaaacag ttaatcagtt ttttgaacag tatcttccga
     2461 aagtgagaat aatgaaccct gaaggaactt acttaatgtg gttagatttt accgcctata
     2521 tttcagacga ccaagaattg gagaacactc ttgttcggaa aggaaaagtc gtattaaatc
     2581 ctggcatcac gtttggtcct tccggtcatg ggcatatgcg cttgaatatt gcttgtccag
     2641 aagaaacttt actggaagga cttaatcgaa tcaagtatgc tttagagaca atcgattaaa
     2701 tcatttattc aaaataaaag gacgagacct tactaggttt cgtccttttt cataattact
     2761 ccgtaccata ccaaactatt ctgaaatttc aattgtttca atcactacat catgtacagg
     2821 tcggtcttgt ggaccacgtt gtacatttgc gatttcatct actgtgtcca tcccttctag
     2881 tacatgtcca aagactgtat gtcggaaatc tagccaagga gttccgccac ctttatacgc
     2941 ttcaatgatt tctgatggga atccagcacc ttctagttgt ccaagcatat tagctggcac
     3001 atttgtgtta tttacaataa agaattggct tccgtttgtg tttggacctg aatttgccat
     3061 agataaagct ccgcgaaggt tgaacagttc acgactgaat tcatcttcaa aactttcgcc
     3121 ataaatactt tcgccaccca ttcctgttcc agttggatcg ccgccttgga tcatgaaatc
     3181 aggaataacg cggtgaaaaa tcacgccatc ataataacct tttttggaaa gttcaatgaa
     3241 attttgaacc gtcttcggtg ctagttcagg gaataattga acagtaatgt caccacggtt
     3301 tgttttgata acagcttttg gtcctttttc actttttaaa tctagttgtg ggaatgccat
     3361 attgtttcct cctaatattt gcatacttca taataataat aacataatac ctaacctctc
     3421 gtaaaaatac tagatttgtt ttcttttatt tttcatcact agatacattc tataacttac
     3481 cgtttcgatt tcgaattcaa acatttttga gaagcattct caattaaatt ctgctatagt
     3541 gtgaaaaggg aggaattttt atggaaatct atgacattac aatcatcggt gcaggtcctg
     3601 tagggatgtt tgccgctttt tacgcaggaa tgcgccaagc taaaacaaaa atcattgaca
     3661 gtctacctca acttggtggt caattagcaa ctttgtatcc cgaaaaatat atttacgata
     3721 ttcctgggta tccagcaata aaagcaagtg aattaatcga tcaattagaa aaacaattaa
     3781 ctacttttaa ccatacattc catttaaaag aagaagttct ctcgcttact cgtgaagacg
     3841 aagttatcga gattactaca aataaaggca tccattattc caaagcagtc attttagctt
     3901 taggaaatgg ttcattccag ccaagaaaat tgaatttaga taatgctgaa tcatttgaaa
     3961 accacggatt ggattatttc gtgaatgatt tgatgaagta tgctggaaaa aaagtcgcta
     4021 ttgctggagg tggtgattct gctatcgatt ggacgttgat gttagagcct attgcaagcg
     4081 aagtttactt gattcatcgt cgcccggaat ttcgtgcgca cgaacatagt gtatcacgct
     4141 tgaaatcttc atctgtcaac ttgcttactc cctatctcat cgatggatta tcaggcaata
     4201 atggggaact gacagatatc cggttaaaaa aagtgaaaag tgacgagacg attgatttga
     4261 tgattgattc tctgatcgtc aattatggat tctcttctaa tttagaacat ctttcttctt
     4321 ggggacttga tagtaccaga aatgccatta ctgtaaaatc agatatgtct acttctattc
     4381 caggcgttta tgcagctggt gacatctgta cctatgaagg aaaagtgaaa ctgattgcta
     4441 ctgggctcgg tgaagcacct actgcagtga acaacgcttt acactatatt aatccaaagg
     4501 aacgaactca gcctggtcac agcacaagct tatacgataa aaatcttcgt cctagttgat
     4561 tttttatatc tctttgcatt tcaagttaaa cccgaaagca gtttcttgca tttttttcat
     4621 gttccgttaa catgaaatag agaaagaaaa aataaagggg gagcaataat atggtcgtca
     4681 aaactgagac tttagaagaa aaggcaagag aactattgaa ggaacgtggc gtaactgtag
     4741 aagatatcgc acaacttgtc atgtttttac aaaaagacta tatttctgat ttgactttgg
     4801 cggattgtac tgaaagtgtc aacagcgtgc taacaaagcg tgaagtacat aacgccatta
     4861 tcactggaat ccaattagat atattagcag aaaagaaaaa attgatgagt ccgttgcaac
     4921 atattatcga agatgacgaa ggcctttacg gtatcgatga gatcctagcc ttgtcaatcg
     4981 tgaatgttta cggtacgatc ggctttacga attatggtta tatcgacaag gtgaaacctg
     5041 gtattttaaa agaactaaac agccacgatg gcgttcatgt ccataccttc ttagatgata
     5101 tcgtcggagc gatcgccgct gctgctgcca gtcgattagc acacaaagaa ccagaaaaaa
     5161 gaagtcgcct tactcaataa ttcaacaaaa aaagaaagaa aacgtctatt tagacgagtt
     5221 ctttcttttt tgtttgtttc aagctatttt ttcctaagaa aagaaaaaca gcaaaaaata
     5281 attctaataa tacaaaaaat aagataaagc tatgcatgaa aaattcttct ggaagtacat
     5341 tgatgattgg atcagtaacg ggatcgaata accagtcatc gttgttgaaa aacgtttcat
     5401 gaaaaaggat aaaaaagcga tcaaatccta tcatcatgaa aaaaccgaaa acaactggca
     5461 gtaacatgcc gatttgaaac ggacgaatca accgccaaag ccgatcattc ttttgcaaat
     5521 atttgataaa caaaatactc gggatcaaag tgatgaaaaa cacagcataa tccactaaaa
     5581 acaaaatttt cacttcataa aagtgatggg ctccgctagc agaaactggg aaatctggca
     5641 agctgaggat ggttttgaag ggattattga gataattcat taggtgatca aaatttttca
     5701 gtaacgtctc ttgatctaaa gaagtataat ccaaaatttg caaatgtccg atatcaaata
     5761 cgtatagtgg tcgaaagttg atcgtcacac caactgccag actgatcagt gtcagaaata
     5821 ggctgaacaa tccagcatat tccatccact gccatctcga atttttcata ttagaagctc
     5881 cattcatcca gtgattgttt cacataggta gctgggatag gcaacccagg tacatcttct
     5941 tcttttgtaa aacctgataa aacgagcaaa gtatctatat tattgcgtat cccagcttgg
     6001 atatctgttt catagttatc tccaaccatg attacttctt ctttttgcag ccctaatact
     6061 tcaacggctt tatccataat gatggcttct ggttttccga tgtagatcgg cggcgtttgg
     6121 gttgctgtct ctacaaaaga gatgactgac cctgctccag gaagaaggcc gcgttcagta
     6181 ggtatattct tatctgggtt cgttccaatg aaagtagcac ctttttgaat agcaagtgtt
     6241 gctttaacta ctttttcata ggtcagataa ttatctaagc ctaccactac atagtcagga
     6301 gcctcttctt cccatgtaaa tccggcagct aaaatcaagt caatcaatcc tgcttctccg
     6361 ataacataga cttttttccc ttttccatct gctttcataa aatcgattgt tgccaaagta
     6421 gctgtgtaga ctgtctctgg agctacatga atgtcaaact catctgctag acgattagct
     6481 actgtttctg gtgacttagt cgtgttattg gtaacaaaca agaaaggtaa ttttcttttt
     6541 tgcaattctt ccacaaatcg tttaccggcc ggtatgggtt gtgtcccacg ataaatcgtt
     6601 ccgtctaaat caattaaata tcctttatag tccattcatc cactactctt ccctttttct
     6661 gatcgtaaat gctgtttttg ttttttctac ttgtttaggt tgttcttgtt tttttacttt
     6721 ttcagaggtt tttgctgact ctgtttttcg atttttaata accggttttt tgcgattaga
     6781 tgcgactggt ccttttttct catcgatata cgcacgatta tggttttttt tcttttttcg
     6841 gcgctgagtt gtcttttctt ttttaccgcc gattctctcg atgacaaaat acgcacaacc
     6901 aaaattgcaa tattcgtaaa gatagtcttc caaggaatcg attcgctgat ctggatgcgc
     6961 ccgtcgatta tccgcttgga aaaatccttt taggcgtagt tgttcataac cccaatctcc
     7021 tacgatataa tcatatcggg ctagtacatc actgtaacgt tctccaagct tttctgcatt
     7081 aaatccttca cgataatcgg acacaagccg ataatgtctt tcacctatca aaaaattact
     7141 gtcatctaac agtgtcacta gctcgccttt gactttttct gctttaggct cctcaacgat
     7201 ttcttccaaa gcagctgtca gttcttcggt gactgttgtt tcctgttcag ctgatgattt
     7261 tttcttagca atttctgttt tatgctcaac gttctgctgt tcattttgtt ttgcagacat
     7321 gcgttcacca cctatctttt ttattatacc ttgtttttat ttgattgggt agtgagcttt
     7381 caagtaatct cctaccacac ttcggataaa ttcagggaac aaaaattcat tttttccagc
     7441 aatctcaatc gttggaaaaa atggaacaaa catcaaatga tctacggtag taaaagtata
     7501 atttttattc agatcgattg gcttgttctt ccaaaagact tgatggttaa tagaatcata
     7561 tgtgattcca ttatagacca tctgcccaaa aatcttaccg cgaaatccca ttcccttcat
     7621 aggatagtta cgtaaaaagt tacgattttt ttctatttct aaaactaagc ggatcagatc
     7681 atttccaccc aatgtgacat ttaacaagtg catcggatgc ggcaacgcag tgtgaagctg
     7741 atcctgattg acccgccctt ttggaatttc agttaaaaac agaccactgt ttaatattgc
     7801 tgcttccgtc tttcctcgtt ctttcaccgc ctcaaaggct acttggataa acgaatgctc
     7861 attgaagata tctagactca gagaaaatgg caaatctgct acttcttttt cttttaataa
     7921 ttcatgccca cgttctagat aactatttat ttcttgttca tcttctgtaa aagctgtcat
     7981 tgtttcggta gggatggctt tggctgaatg cttaaggatt tttttctctt catcgatggt
     8041 taaatggact tcaccgacat actgcccgta ctttccagcg gctgctattt ggacatcatt
     8101 gattacgtga ccttctctga ataaatgatg ggtgtgcgat cctaaaataa catcaattgc
     8161 tggtatttct tttgcaattt ggaaatcatc ctgaatcccg agatgactca tcaaaactaa
     8221 aacatccact tgtgaacgta aatcttcgac taattctggc aaaatatcat aaggattccg
     8281 tatgtcccag ccattcggat tataagtaag cggaaatgga gcagtaaatg caattagtcc
     8341 gattttcgtc tggtgtttcg ttgtaatgat tttatatggt ttcgtccatg ccggtggctg
     8401 gagcgtattt ttatcgaata agttatccag gatgatatca aactcagcat gatcgtataa
     8461 atgattcaac tcttctttgg aatttcctac gccttcattg tttcctatcg tcgctgcatc
     8521 ataaccaact tcattcatca gcaagatatt ttcctgtcca ttcgttgctt ctgacaatgg
     8581 atgccaacga tccacaaaat cacctagatc tactgcaatc gttgtcgttt tttctttctt
     8641 ttcgttttct cgtttacgtt gatccaaaaa ccttctgatc cttggccaat tttctaaatg
     8701 agaatgcaga tcattcgtat gtaaaatcac tattttttcc atgtaccctc tcgcctctct
     8761 ttatctttat cataccatag gcagcttttt cttccattga aacaagctct atttcagagt
     8821 ttttcctgcc ttgaagcaaa ttgtataagt acaatattcg ataaatcagt ctggctaaaa
     8881 aatcaggaac attcattttt acagtactat aaaaataaaa aaaacagacc cgaaagtctg
     8941 ttgtcgtttc attataatca gacacatggc ggaacctgct tagtgctgct tccttccaga
     9001 cctgacacgc ttcacaagcc caccattgtc ctagcctttc ggcaaagaat agtatacctt
     9061 atttttcatt cgttgaccag tcttttttta atttcttttt tcgctggcgg agctttcgga
     9121 aaaagtcagt caaaatcatc ccacattcgt cttctaaaat gccaccttct acatatgctt
     9181 ggtggttaaa ccgctcgtcc tccaaaagat tcatcaaggt accggctgtt ccgccttttg
     9241 gatcatacgc gccgaaataa acttctgcta cacgagacaa aatcatcgcg ccgctgcaca
     9301 taggacaagg ctctaaggta acaaaaagtg cagcatcttc caagcgccag cttcccactt
     9361 tttcacaggc ttccttgatt gcaagcattt ctgcatgtgt agtagcatct tgcgtggttt
     9421 ctctaaggtt atatcctctt ccaatcactt ttccatccaa tacaaccact gctccaatcg
     9481 gaacttcatg aagcatttct gctttctttg cttctgccaa tgcaaggcgc atccattttt
     9541 ctttttctac tgcatcaaga tttgtattta ccaattaccc ttccctgcct ttgttttttc
     9601 ttgttttact ttagcatatg ataaaaaggc tgtaactaac ttttttttct atggtaaaat
     9661 aaatacaaga ggtgaacaag atgaaaatgg ataaattact tgctctttac ccacaagcac
     9721 agaaaaaaaa ctctccagca tcagaagcgg atatcctttc ttttgcaatt gatgatgctt
     9781 ttatctggat caaacaagat tcattatctc aacaagaaac cagccttttg aaagcgttat
     9841 tccctgtaat caacgatcaa aaaaaacacc catggtatca ctatcttttt aaagatgctt
     9901 cttgtgttca agaaaaaagc tttcgtttga tccagcttca cgtcgaatca aaaggagaat
     9961 tcttaaaaaa agaatggcaa gatactattt tggaattatt tcgctattct gctgatttct
    10021 ttttttatac agacacagat gcgctcttag tcgaacaaat aaaccgtgat tatcttgatg
    10081 ctactgaaat caacggtatt tttctatctt tagatgctga ttttgatacg acaacgtctg
    10141 tattcgttgg ttctttccat tcacctggca ccgacctcgt ccctttgttt gctgaagaaa
    10201 gaaaaatttt tttaacagag caaaacaatc tttatactca cagccgtact ttttctatgc
    10261 aagatgtggc tttacattac tatacaaaag atactgtaaa agatagtaag ttgatcaaaa
    10321 gttacataga actgatcaaa caaaatgaaa tggaagtgat cattcaagaa ttatggaatg
    10381 aattaggcaa tgtcagctct gctgcaaaaa cactgtttct tcatcgtaat actctaaaat
    10441 atcgaatcga aaaatttcaa gaacagtctg gtttcaatct aaaaaaagcc aacgatttac
    10501 tgttttgcta tcttttattg cttcatgaac ataattaaag atagattaaa ggggttgtga
    10561 caataatttt gtcacaaccc cttatttcga atgaatagtg ttcaaaagct agtttctctt
    10621 acatcatttt ttcaacaaaa tgcattagcc ggtcttttac atcttcggtt gaatctttga
    10681 ttgaatcacc ataaacagag attttctttt ttcctgattt gatttttgct aacattccca
    10741 tcaaagaatc caagtcacta tctgaaagac catctacgat ttctagtaat ttttcatttc
    10801 catcaaattt gtcatagacg aattttttta ctttcgtccg agtagtcata tgatggatct
    10861 tttccattac ttttcccgat acaacgactg ctgtcgctac actggcagcg gctgctacgc
    10921 ttaatccgat tgttactttt gttgatgttt tcataagtat cagctccttt ttatctatct
    10981 ccatcatagc atagacaaaa aaataaacga actaatccac tcagtgaaat ctcaatcctt
    11041 tagggaaaaa gttctttact gttcctttta cttgtttccc aaatccgaca ggtatcttat
    11101 ctaatactag caaaacccat ccttgattcc catcacgttg aaatgtttct cctgctgtgt
    11161 aagattgcca ttccttttga gtgatcggca agcaaggtat attctctatc ttttttgtag
    11221 caagcgctaa tgcatagcta ggttcaaatc gattcttttt gaaatctcct aaatgcaaac
    11281 ctgtacgtac gacttttaaa ccatcgagag aaggggctaa ctcaggaact tcccaaagat
    11341 ggtcgttgaa cactaaaagt cttccagtag cttcataatg aaaatcattt gaaaactccg
    11401 tccagagttt ttcctgctct tttgtcattt gcactttact tttttttcgt gtcttctttt
    11461 ctttatgcat ctgattttgt ccatgaaaag tcagctttgc aacaaaatgt ccttcaccct
    11521 gatctttatg aggccatata cgtattgttt tttccaaacc agctacgctt ccccattcag
    11581 aacgtcctga actaacggat tgagtcaaag gaatctcctc aatcgttact ggataatttt
    11641 ctacaagcca gctaatgatc tcttcatttt cttctggtgc aaacgtacaa gtcgaataaa
    11701 tcagttgtcc tttatttttt agcattttta ttgctgaaga caaaatctct tgctgacgtt
    11761 tttgacagta tagcggagat tcttctgtcc actcttttat tgcatttgga tcttttcgga
    11821 acattccttc tcctgaacaa ggagcatcaa ccacgatccg atcaaaaaag cccgaaaaat
    11881 gagggacaag ttctgcgggt gcatgatttg tcactattgc gttcgaaact ccccatcgtt
    11941 ctatattttc tgataaaatc ttcgcacgtt tcggaaagat ctcattcgtc actaataacc
    12001 ctttcccctt catttgagca gctaattggg ttgatttacc accgggagcc gcacacaaat
    12061 ccaaaacttt ttcaccgggt tttgctgcag cagctgttcc aacgatcatt gcacttggtt
    12121 cttggctata ttcataccca gcttgatgga ggaaagactt tccattgaca gtgccaagaa
    12181 atccctcatt gctgtaggga gcgggttgta attcttcgct atggtaagtt tggaccatgt
    12241 ccaatcctgc cggttttagt ggattccacc gaaatccttt tttcacgctt ccttgttcaa
    12301 gcgcagaaaa aaaatcggat gcttcttcgc ctaaaagaag acgatatttt ttgataaatt
    12361 gctgtggtaa cgttgtcgct tcctccttca ttccaatgtt tcgttagaca ttttacaatc
    12421 ttttttacga aaaataaagg tggctgatct actttttcat gttgctgcct aaggtgactg
    12481 ttcctttaag atattttcca agtattcatc cgtcacctcg tttttatccc aaacagcgtt
    12541 catcatttca agaaaatctt gagaagaatc atctgagcgc aaacttccca atgcattaag
    12601 ccataccaat tcaagcgact tttcttcttt gatcttcgtg tgacctttga catatgagaa
    12661 aaatggccgc ttaaatagcg aatccatgtc taaatcagat tttgcaatga tttcacgaat
    12721 actttcatgg atagaaaaaa aatctttcaa taagtagaca atgcttaaaa catcatcgga
    12781 actgcattca gctaagtagt tttcaaaata aacagtgatt tcttctaata tcttctcttt
    12841 taacccgctg attgatttga aatgtgtata aatcgggtat gtagaacaac cgcagaactc
    12901 tgctaactct cttgcagtaa ttgacttcat tccatattct tccacgtata ccatactttt
    12961 gaacagcaaa tattctttac tataatttgt tactctcccc atcattctta cctccgtctc
    13021 ttttcatcta cccataaata aataacataa ataattctaa aaatattatt gataattatc
    13081 aaaacatatt tagttatatc cattaaaaat caatttttcc gccttaaaaa caagaatatc
    13141 ttgacaaaat accctaggaa ataaacaata gaataaacaa ggtaacaaaa aaattaaata
    13201 acgttatata aaaaagaagg cgacactctg tgagaataac acagactgaa atgactttcc
    13261 actcacagtt tttgtctaac tatatacaaa aacagaataa aaaggaggta tttatgctaa
    13321 ttgaagcaag aaaaaaagag ttacttgttc tacaaatggt cattcaaaaa aataattgtt
    13381 ctttacaaga attatccaat gacttacata ttccaaaacg gacaatcaaa gaactgatcc
    13441 gaaaaataaa tgtatcactc aagcaatttt tattgattga tgaatttatt tattctaatc
    13501 ataaaggaga gatccaaacc aaagaaaccg atcaaactaa aaagttagct gttttttcta
    13561 agttaaaatt atgctaccta aaagaatcca atcgttttaa ctatttgatg ttactaatca
    13621 attatccaca aactgctgta ccaaaaaaat atttacttga acaactttat atatcgccct
    13681 cctatctcga aaaactgaca aaacagctca atcattcctt aaaaaaattc catttacgga
    13741 tcctatctgt aaaaaattgc tgtactcttg aaggaaatga gttatctatc cgtctctatc
    13801 tttacttttt tctttcagat acatttggag gtaacgattg gccattttcc tcacttgaat
    13861 taaatgcact aaaaatgaac agtacagatg agcaaataga aaaaatttca aaacaagaaa
    13921 aaaatatcta cttgctgtta actttattca tattacgaaa caaacaacag gcatattttc
    13981 caaaaacttc ttctgaaatc actgaattga tggacttatt gcagcataca catgattatt
    14041 cagaatactt tacttctttt tcagtgagtc gcccttgtga agagacagaa acagagagag
    14101 cctactttaa ttattttttc tattgttttt ttccaaatca aatctctttt cgacaaaaac
    14161 aggagattgg ttttttattt tccacaagca aaaaccttat ttgtcgacaa gtcggtcaat
    14221 tgatgactta cttaactcaa cagtttcctc tcaaccattt agaaaataaa ataccaattt
    14281 atttttattt tcttgtgttg gcagttgttt ttttcgaact gttgggtgaa tccagcagta
    14341 gtttttctac actttgcttt ccagaagcca cgtccaatca ttctagttct tttatacagg
    14401 aaaagaaatt aagagaaaaa atcgatacaa ttttttcaaa aaatggggaa agaaaccaaa
    14461 tcagtcaata ccttagtcaa ttgatccaac acttactaca aacagaaacg aaagagcagt
    14521 tactcatcca tttgcagata accaaacagt ttacaggaaa ttatcatctt caaagccatt
    14581 tgcagcagtt atttcgcaaa gatgctgtta aaattaccgc atattcagaa catgctgatt
    14641 tgatcattac agatcagcat gagaaaattt cagatgaaac ggagactttc tatctggata
    14701 caacgcacaa tacagtgtta tttggcaagt taataaaagc ggttcatgaa ctgtatatga
    14761 aaaaaggtta tgagaacaaa acaaagagga tgtgacaaaa ttcttgtcac atcctcttat
    14821 tccaaataaa tggtattcaa tcagctaact ctcggtatta gctcagatca aagcgctgat
    14881 ttcacaactt ctttatttta atctcttcgg ttgccgccga acaataagat caagcgcaac
    14941 agctgcagga atgtcgaaag cgctgctgca acataagtca gtgctgctgc aaataatacg
    15001 tgtcttgcca tcggtacttc ttcttccgtc agtagtccgc cttcactcaa aatttggatt
    15061 gctcgccgag acgcgttaaa ttcgactggt aatgtcacca attggaaaag taatgctaaa
    15121 gaaaaagcga aaatcccgat attgattaac gtagagttcc agctaagcaa tacacctacc
    15181 ataatcaagg ggaacgaaat catcgaacca aagttagctg ctggaacaag tgctgcacga
    15241 atcttcaatg ggacataacc cgaagcatct tgtaccgcat gtccgcattc atgagctgct
    15301 acacctatcg cagcaactga agtagactgt gcagttgctt gagacaaact caacatctta
    15361 ttacctgaat tgtagttatc tgtgagatct ccggcaattt gttgaacacc gacattattt
    15421 atctgttgac tttgtaatat atattgtgct gcttgtgtac cagtcacatt gtttttgctt
    15481 ctcacttgat cgtattttcg gaaagtacta ttgacgtacg ctgacgcaat tccggaaata
    15541 actaatccaa gaatgaccaa aagaaatgtc ggatctaaaa aaccatacat tccataacca
    15601 tacattttcg tttcactcct tttcgtttac tttattttat cattagtagt aggaaaagaa
    15661 tgtgaaaaga ccttgaatat tctttaattc tttttcacaa attccgattt cagtttcatc
    15721 ggtccaaatc cttctacctt gcaatcgata ttatgatcgc cttctactaa acggatgttc
    15781 ttgacttttg ttccttgttt aatggcacct gacgcacctt ttactttcaa gtctttaatc
    15841 actgtcacgc tatcgccctc taacagacgg tttccatttg catctaatac cactaatccg
    15901 tcttctatga cagtttcttg ttcttctttg ttccattcgt tcccgcattc tgggcaaatc
    15961 aacaagcctc gatcttcata tgtgtactct gagccacatt ctgggcaatt tggtaatgtc
    16021 atcttctttc tcctttaaaa tctttagtga gcactattgt aacggaaaaa gaagatcttg
    16081 aaaagataca tttgtgattt ttactacaaa aggcacgttt tcgaacgaag tatcattctg
    16141 tttttctgct cttatacgat tggcatatat cttggatctc tttatagatt tcctctactt
    16201 ctgctaaatc ttttgcattc gcaccacttg ctaatggatg gcctcctcca tgatggcgtt
    16261 tagccaattc attgatgatc ggccccttag aacgtaaacg tacgcggtaa taccctgaag
    16321 gctgctgaac aaaaatcccc caagccagca cttcgtcaat cactccagga agtgaaacga
    16381 tcgaagctgt ctctgaatca tcgattccat atttatcaag aatttcttgc ggaaggatta
    16441 ttcttcctgc accattttca tccacctgga tattttgata aagataaccg gaaagtttag
    16501 cgactttcat cggcatttgt tcaatctctc tgttcaaagc acttgcatca aatggataat
    16561 ttctcaatat cgaagcaatc tctaatgtac gggacgtcgt ggagggatac aaaaagcgcc
    16621 ctgtatctcc gacgattcct gcataaagta accgtgcagc cttaacgttt atctttaaag
    16681 attcaggaaa catcaaagca aattctgcaa tcatctcact acagctactt gcttttgtat
    16741 ttacccacac aagatctcca taagggtcat cattgggatg atggtcgatt ttgatcagtt
    16801 tttctgccaa ctgatatctt tgatcactga ttctgggtga attagctgta tcagtcacga
    16861 tgactagcgc tccttggtag aagacatctt caacttcttg catttctgct aaaaagcgaa
    16921 gcccttcaat cgtttcacct acttgacgta tttctttttc tgggaaactt tcccgtaata
    16981 gttcggctaa tcctacttgt gatcctaatg catcaggatc agggcgcaaa tgcctgtgga
    17041 taatgatttt atcgtatttt tttattgtat ctactatttc ttgctgtact gtcatttcac
    17101 tcctgctttc taagaacgtt ccattacttg acaaacaacg attgctttgg aaacgatggt
    17161 gttttcaata aacacttcga tatccagttt agcggaacgt ctgccgattt ccaatacacg
    17221 tggacgaatg tctaattcgc tttccaattg gatcagccgc aaataatgta ggttcacttg
    17281 ttcaatcaag atatttcgtt tttggttcat caccattgtt ttttgtgcga catcagaaat
    17341 gatttcgcta agtacgccaa aagaaatggt tccaacgcca ttgaccattt gtggtgctac
    17401 tacgaatttg aacttagggt tttccaaacg atttccttca cggtcagtat caatcgtatg
    17461 gatatttcca gatatctgat ccgaaatcgt atctgcaatc tgcggttgtc gctggacaag
    17521 ctgcatcgct ttcattacat cttgccgggt aatcaagcct tccaatgtca agtcatcgga
    17581 aacgaccggc atgacttcta gcccatccca gatcatttga tggctaacag aggcaatact
    17641 cattgttttc tttacactga ttggttcttt cgtcatcacc cgatcgatga tctggtttgg
    17701 cgatttccct aaaacatctt ttgccgtaac aatccccatc aaacgatgat ggcggttgac
    17761 gactggaaaa cgcgaatgct gcgtttcttc tgacaaacgc tgataatcag aaatcgtatc
    17821 tgtctgctgc aaataatgag tcttttcagc tggcatatag atatcactga caagcatgat
    17881 atcttttttg atcaattggt cgcttaacgc tcggttgatc atggttgcga ctgtaaatgt
    17941 atcataagag gtgcgtaaga tcggcatttc aagatcatca gctagctttg caatctcagc
    18001 agtcgtattg aatcctcctg tgatcaatac tgcggcgcca tcttccaatg ctaatttttg
    18061 gacttccgcc cggttgccga cgatcattag tgatcctggc gtaatatagc gttccatggc
    18121 atgtttagtc atcgcgccga tgacgaattt attcaagacc ttgtctaatc ctgccgcccc
    18181 gcctaaaacg tccccttcaa tgatcctgac gacctcgcca aatgtcaaac gttcaatatg
    18241 tttttttaat ttgcgttcga tacggatggt ccctactcgt tgaattgttg aaactagtcc
    18301 gacattttct gcatctttga ttgcacggta cgctgttcct tcactgacat tcaattcttt
    18361 tgcgatgctt cttacagaaa ttcgattgcc aacaggtaaa ctttcaatat attctaaaat
    18421 caaatcatgt tttgtcgcca ttttctgcct acacttccag cgtttccgag acgctcatta
    18481 tttttccttc actatcttcc agtaactcta caaaagattc gggatcttgt tcaatgacag
    18541 ggaaagtatt gtaatggatt ggaactactt tcttggcacg aattgtttgc gcagcctttg
    18601 ctgcatcttc tggccccatc gtatagttat cgccaatcgg caagaaagcg acatcaatat
    18661 cgaattcttc tgcaagcaat cctagatcac tgaaataagc agtatcccct gcatgataaa
    18721 tcgtttttcc ttctgcttga ataataattc cgctaggctc gcccatatac tgaacgatac
    18781 cgtctacttc atagcttgaa ctatgctgtg catgtacgaa ttttaccacg ccgaatggaa
    18841 acgcatgttt tccaccgata ttcataccga atgaatctac tcctctgctg ttcgcataat
    18901 cagcaatctc aacaatactg ataattgtgg cttgattttc ttttgcaata ggaatcatat
    18961 ctcctatgtg gtcactatga ccatgagtaa tcagcaacca atctgtcttt acttcttctg
    19021 cagttaagtc tgtcaatgga tttcctgtaa taaacgggtc aatcatcacg cgttggccat
    19081 tttccatttc aattgttaaa caggaatgtc catgatacgt aattttcata ttttacacct
    19141 cgcgttttct ctgtatcttt tttattaaaa acgatttcat cacttgcaca tcaatgaaaa
    19201 aatatagctg ctaaatatat tttcattgga ttgatagtac agtatgtaca aagcaattgt
    19261 aacagataat acaggatgca ccaagtacca agcagatata atctcaagtt tgaggtctct
    19321 gctctgctta gcttctcttc tcgtccctta actaattaaa tcagcgtaaa taggatggac
    19381 gatcttttct aaatcggcta cttttatccg aaggcttctc ccgatttcac cgcttgacac
    19441 aattacttct tctttttctt ttaaagaact gtctgctaaa aaacaataat caggatgtgc
    19501 gagttttatt ccaatagggg tgtttgctcc gtggggatat cctgtatgca ttaatacaaa
    19561 atccattcca ggaaaaccaa ctttacgatc tttcgtcact tgtctcgttt ttttgtaatc
    19621 taagtgatcg ttcaaaggaa tcaatgcgat gatcacccca gttttgtttc ctttcactac
    19681 gagagtttta catatcaaag ctggatcaat ccctcttttt tgtagttctt ctgtgaaaga
    19741 ttcttctacc acatgactaa aatcaagtgg ttgatagctg atcttttgtt cttctaaata
    19801 acgttcgact tttgtttgac tcatcattga ttcactccct ttattctatc gtttattata
    19861 aagcgaggga tgctgtattc aaaaattatt tgtttttcgc aaaaaaagag caagacgagc
    19921 actctataga gaggttcatc ttactctttt tgtttatttt ttatttcgac gttgagtagc
    19981 ttccataatg actggcaaga tcactgggcg gcgttttgtt tgttcaaata agtaacggcc
    20041 taattgatct cggatgtctt gctttaattt gctccactca aaatcattgc tttctaaatg
    20101 tttctcaacg attgtttcaa tcatattgct gctttctttc atcagatcac ggctagcttt
    20161 tacgtaaacg aatccccgtg aagtaatttg aggttttgaa atgattttct tttctcgccg
    20221 actgatagtg acaaccgcta cgaaaatccc gtcttctgac aagatttttc gatcacgtaa
    20281 aacaatgttt ccgatatcac ctatgcctaa tccatcaatc attacatttt ctgcacttgt
    20341 agttcctgca acactcatac gcttgttctt atattccagc acgtctccgc gtccagtaat
    20401 atagatattt ttgtatggca tccctactgc atgagccaaa tctgcatgag cagctaattc
    20461 acgatactct ccttgaactg gcacaaaata tgttggtttc atcaaattga tcattaattg
    20521 caagtcatta ggattggcat ggccagaaac gcgcatattc tcagagatca acttcactgt
    20581 tccaccagct cgataaatga tatcttccgt tttcgccacg atggtttcca tcgcgatgct
    20641 tggcgtagta gtgatataaa ccaaatctcc ttcttcaatc ttgacattac ggtgtgtacc
    20701 gtttgccatt ttttgcagag acttgatggg ttcacccatt cggccagttt ctaaaatgat
    20761 cagttcttct ggatcatagt ttttcatgtc tttcaacgta acaagcagat cttcacttgg
    20821 taattgaagt ttttctaaac gcatcgctgt gcgaataatt cgttcgaaat cttgaccagt
    20881 caagacaact tttcgttgtg ctttttctgc tgcatccaat acttgttgca ctcgttgcaa
    20941 attacttgct acacaagcga cgatgatacg cccttcccaa tatttgatcg tatcaaatac
    21001 ttcatctgct atttgggctt cagaagctac aggctgagga ttttctgcat ttgccgattc
    21061 acttagtaag gctaagactc cttctttgcc gatttccgcc aatcgtgcat aatctgtttg
    21121 atacattggg atagccgttt ggtcaaattt aaaatctccc gtataaacga tactaccttc
    21181 ttttgttttc agattgatcc ccactgaatc tggtatcgta tgagtggtac ggaaaaagct
    21241 cactactgtt tcaccaaagt ctatttctgt atgctcatct accacatgga aatccttaaa
    21301 tcctttggct gcttcattac gactgacatt caattttgct aactcgatcg tcaactctgt
    21361 cccgaatact ggtacagaaa tttttgacaa caagtaagga agtgccccga tcgcatctgc
    21421 atgtccatgt gttaaaaaga caccagctac acgatcaatg ttttcttcta aatacgtaaa
    21481 atcagggatt acgacgtcga tccccaacaa ctcattttca ggatatttta gcccacaatc
    21541 taaaataaaa atatcctcgc ccacttctgc gacatacatg ttttttccat tttcacgaac
    21601 gccgcctaat gggataattt taatggtact cacaaacttc acctcttctt atgacatcca
    21661 agtttaaaga tgtcgatttt cttctttatc taaatatatg agccattcca atgaagcata
    21721 tgcaaaatca ctatttctca acttatggag aatcagcagg aaaattacca gcacatccgc
    21781 gttctgacag ttggatgggc attttatttt ggatatactt cattaacaaa atggatcttt
    21841 tgcgaaacgc tatagccaga atacttatat aagtattcag ctgttcccca ttattataca
    21901 acaaataaac taaatcgcta gtatcagcaa ttgttagaac aactatgcac gtaaaaatcc
    21961 ctctgtagca tctaggattt cttttgctat tcctctttca tttcttcttc gtcggtttga
    22021 aacatacttt tttcatttta ccacattttt tgcaataact atagtgactt tttatatata
    22081 cagaacctca tgttgatggt gtgcaaacag cataagattt aagctagctt taagataggt
    22141 tgtctattat caaatgcatc tactaggagg gatgcaaaat atgaatttta tgaaacgtgc
    22201 atggaaaagt acaaaagtaa aatggggacg ttcattgtta ttgtttgccg tgttcacagc
    22261 cattcttatt tttgtgctag ctggcctcac gattcgaagc gctgcagaag aagcagcgaa
    22321 tcaagcacaa aaagaagttg gagcaacagt gactttaagt gccaatcgag aagcttcatt
    22381 taaaaaacag gaacaaactt cttcaagcgg ttctaccact gaagaacgtc cagatccagg
    22441 cagtttttct cttacaccag tttccttaac ggacgcggaa aaaatagctg aattagataa
    22501 tgtaaagagt tattcctttg aaagttccgc ctcagctatg gcaaaagatg gaataactgc
    22561 catcactagt tctgattcaa gtgaagacag ttcagaaact actgatgcag gcggtgaacc
    22621 tgctggagga atgggcggga atccgcaaat gactcaagca gactttcaaa tcagtggtgt
    22681 gtcagcaact gcacaaaata gtaatttttc tgatggaaca gcaaaaatca cggatggtga
    22741 aggaatagat gacagcgaca aaggcacaaa caatgtagtc attgattcta ctctagcaga
    22801 aacaaatgac ctggcagtcg gagatacttt cacaatcacg agtactgaag atgaagatac
    22861 cacttatgaa atgacgatca aaggaattta tgaatccagc gaaacaagcg gcagtatggg
    22921 tatgaaattc aacttcatga atccgtcgaa tacactttac acttcttaca cttttgcaaa
    22981 tgaactaaat gggacatctg acgataacac gatagattct gctatttaca ctctatctga
    23041 tccagataaa atggatgagt ttctcaaaga agctgagaag ctgatcgaca cagatacatt
    23101 tagcttacaa tcaaatgatt ccatgtatca gtcaatgttg gaacctttaa acaacgtggc
    23161 aagtttttct aaaaatgttg ttttactagt ggctgtagcc ggtattatca tacttactct
    23221 gatcattatg attaccattc gggagcgtcg gcatgagtta ggcgtcttgc tatctttagg
    23281 agaatccaga agtaaaatca ttctccaatt gtttaccgaa gtggctattt gtatgattct
    23341 tgcattagga gttgcttcgc ttagcggaaa cgtcgtcgcg aatgccgtgg gacagcagct
    23401 acttgaccaa cagacagaaa ccacgacaca aaaccagcaa gctcctggcg gtggacaaat
    23461 gccaggtaac agacaagaac aaggcggtca acgcgatggt caacaaggaa ccacgaataa
    23521 tccatttgct gtttctgatc aagtcagcga attggaaatc aatgtcaaac ctgcacaatt
    23581 gggactatta gccggagtag catttggaat cagtctgttt tctgtctttt tagcttctat
    23641 agggatatta agattaaacc caagaaaaat cctattaaac taaaggagga atttgatgtt
    23701 acggacagaa caattgggat tttggtatca agatccgcaa gatgcgcttt ataaagatgt
    23761 agaactgaat ttcgaagctg gaaaaatgta cgctatttta ggagcaagtg gttctggaaa
    23821 aacgactttt ctttcgctta tttctggact agatgcacca aaagaaggaa aggtatacta
    23881 tcaagatcgt tcactgaaca aaatcgggtt aagaagttat cgtaaaaatg atgtctcgat
    23941 tattttccaa gcttacaact tgcttcctta tttatcggct ctagacaacg tcatagcagc
    24001 aatggaaatc actaagacca agcaagagaa ttatcgacaa gttgctttag atcgactggc
    24061 tcgtgtagga attgatgaaa tattagcaaa aaaacaggtg accaaacttt cgggaggtca
    24121 gcagcagcga gtcgctatcg ttcgtgctat ctgctgtgaa catcaactaa tcgtagcgga
    24181 tgagcctact ggaaatctag atgaaacaac ttcacaagat atcgtcaaac ttttccaaga
    24241 aatcgcccat gaacaaaatc gctgtattat catcgtgact catgagattg aagtagcaaa
    24301 aagctgtgat gaagtctttg aactaaaaaa taaaaaattt caaaaaatcg aagtttaaaa
    24361 aacaggataa tgatcaaata aaaaatccga accatatctg tctcgcagat atggttcgga
    24421 ttttttattt gcttgattca attagtaata agtggttttt attttttttc gtcttttcga
    24481 gaaaacttgt ctttgacttc gtcgcctaaa tttcgagctt tttctttgat gtcagcacct
    24541 agtgattgtg ctttcccttc tgtttctttg ctcttgtcgt ctgttgcttt gccgtatgct
    24601 tcttttcctt caccgactac tttatctttc atatctttag aacgtccttt taaatctgcc
    24661 attgtgcttc ctcctcttag tttgtttatt gatctacctt cagggtaaca tcaacaacag
    24721 atacagacaa acagaacgct cgttttctaa aaaagatagt tagagctgtt gattaaaaac
    24781 ttttttcgaa agtcccccat actttgactt ctttttcttg gaccattatt tctcctttag
    24841 cgataactgt atcaatatca agcgtttctt tatccaagat acacagatct gcatcagcac
    24901 caatttcgat ttttcctttt ttatccaatt tcaatatacg tgctggatta ctggttatag
    24961 ctcgtaaggc gatttccaaa gggatacttt ctttttgcac agcttctttg atccctacta
    25021 gcaatgcctt ggcacttcct actcctaacc ctaggaaatg attgttttca tcaaaataag
    25081 gcaagcttcc ttgtccatct gaggtcatcg tgaaattatc taaagaaaca tcttcctcga
    25141 tcaatcgttt cagtgctttg ctgaagcgaa cttcaccatc tgtcttctcc caaaaatcag
    25201 gatcttcgct agcagtgaag tcaatcgtgc caccacgttt ggcaaatgcc acgcaagctt
    25261 caaataattc tggtgtgcga tttgcatgag taggcaaaaa ttgtgtgatc ggaatttctg
    25321 tttcttctac gattcgtgtc aatagctcta gctttctttt tcctccgcct aagtggacat
    25381 tgatgatccc agcttttccg gataacatgc ctcctacacg agcatctgct actgcacgag
    25441 cgaactcttc aaaacttggc tgagaagaac ggtgatcgga tactgcgatt tctccgattc
    25501 cgataatttt atcaattgtc aaaaagtctt tgatgataga atctgttact gtttttaccg
    25561 gcaatcggta cgaaccttcg tatacataag tggagattcc ttctgcttcc agacctttcg
    25621 ctttgctgat caacgcagtc atatctctac cttctccgtc tgttcccaaa caaccgacaa
    25681 ctgtcgttac accagctgta gtcaattggc tcaatgtgac ttctggcgtt cgattttgat
    25741 aaccgccttc tcctcctcca cctaaaatat ggaaatgaca gtcaatgaaa cccggggtta
    25801 gtacttttcc ctcacctgaa agttcttcta cttccactcc ttcgtagccg ccttctagct
    25861 gttcttctac agccaaaatc ttcccaccgg caatcagtac atccattttt cctaagtaat
    25921 ctggtgcaaa cacttcaatt tgtcgaatga ttttcataca tttttcctct ttctttaagt
    25981 ataactgatg ataggatctt aaaaaataat ccaaacgata ggatctttac tcctttgatt
    26041 ttaccgtaaa atgacggttc ttggagtcct tatctactgt ttggattact tttttattta
    26101 caggtctcca gtactactgc agacctattg caatggcaat catgatgaaa atagttccta
    26161 aaacaaacag gaatccttgc atcttgattt ggaatcttgc ccattttgcc cattcgatcc
    26221 gtgcaacacc tagcacacca atcaagctgg ctgatacggg tgtaaaagcg tccataaaac
    26281 ctgaaccaag ctgataagca agaactgcta cttgtctgga tacacctact aaatctgcca
    26341 gtggagccat gattggcata gtcaaagctg cttgtcctga atttgatgta acaaccaagt
    26401 tgaacaaact ttggaagatg tacatcgccc aagcaccaat gacagctgga acaccggtca
    26461 acgccgtccc gatcccatgc aaaatcgtat tcaaagctga aggaacagat gcatcagatc
    26521 ctcccaagac aagaagaatc cctttggcca tacctactac gattgctgta ccagctaaat
    26581 cagctgcacc agattggaaa gaagaagcga tgtcattgac tttcattcca tctaatttga
    26641 aaagaacagc aatgattcca gcaactaatc ccatcacaaa gaattgagaa gcgatttctg
    26701 gaatgtaata gcctttttga gtcacgcccc aaacgatcca gacaaggaca actaacatct
    26761 ctaataagat caatttatgc ccaagcatga attttttatt ttcatcagat gtctttttga
    26821 tatgagaacg gaaataatca tctgatttgt aagtcaacga actttctggt tttttacgga
    26881 ttttttctgc atatatcatc atataaccag cggctaatgc agtaacaact acccacataa
    26941 tcaatcggaa tgtcgcacca gacaacacgg ggattccagc aatcccttga gccaccgcta
    27001 cgctaaacgg gctcatccaa gaggtcgcat ttccgacttg agatgcgaca taagtcacgg
    27061 ttactgctac gatcgagtca tatcctaacg caatgacaaa tggaaccatc accattgaga
    27121 atggaattac ctcttctgcc atcccgaatg tagcgccacc gaatgaaaaa gcaaagaata
    27181 atagcggtaa agctaaacgc tccagtccct tagttttact gataaacgca tagatacctg
    27241 cgtcgatcgc tccggtacgc ataataatac caaaagcacc tccaacaaca aggatcaatg
    27301 cagcgatccc tacagcagat ccatacttat ctccagatac tagtccttca aaaacaaaat
    27361 ttaagaaacc aaagccgcca aaatcatctg ttccccatat tttggctgtt ttatgcaatt
    27421 ttttagaagt atcatagata ttttcgccgt actgatcgta aagcacatca tctgtcaagg
    27481 agatttcatc tagtttttcc tgcgtaagat ttttttctcc gtcagcaatg actttttcca
    27541 atcctttttc cggaacatcc agtttttcac gttctgctgg atgatcttgc agttcttcta
    27601 attgatcaaa cacatacgac ttatccagct cgtaagcata acggaaggaa tcttgacgta
    27661 aaacagtacg agtacttgtt tcgccatttg catctttgta ttcaatatct tgcgtgctga
    27721 actttcctgc tggaacgaca aaagtcagta tccatgcaaa gatgactaca ccaaaaataa
    27781 tgacatacgt atggggtgtc gataatttag tgaagtcgcg tttcggtttt cctgaagaat
    27841 ttgttttctt caaatttatt tcttcctttc ttttttcagt aattttgatg aataaaaaat
    27901 ggataatcaa atgaattata cagtaaaaat gacatttcca catgaataat ttattagaaa
    27961 atcacttttt tctattttgt ttttttctcc tatgtatgat tatttttttc tctttccatt
    28021 tatattaagt attttatttc tctgtttttc cttaaaattc tttttttatt tttagattag
    28081 agaaaatctc gtcctgtttt tttcatttta caatcattga aaaattgaat ttcattgcaa
    28141 aaaaaataat aaacacttat aaatatccat tatctatcct tcaatttttg tgtattttat
    28201 ttctattaga aaaaggtatc ctgttggaca attttgttat atcaggtaaa cttcattgca
    28261 ataatgataa caaatgtact ataattactt tgtaaagagt tttggggaag actctttcat
    28321 caacgtgtat taagagacaa gaaagaatcc atatggattc tttcttgtct ctttttccac
    28381 tactttttat ttttctgaca ctacatattc ttataactca tttctcataa ctgtgaaaca
    28441 acttataaaa ataacaaact ttaaacaaaa caagcttaaa atcaaagatg atccatctta
    28501 aaataactaa aacaaaaaaa gtagtcgttg ttaatcctca ttaataatcg ttttcttttt
    28561 ttcaaacgct ccatttttat gtggaacatt atttttttaa ggaactttgt caacatgtgg
    28621 ataactattt tccacttgtg tcctggacta gctgttttgc tgtaaaatac aaaacttgct
    28681 gcagcaaaaa gatcgttgca agagaggctg ttccgcagat cgctggatct agatttggca
    28741 aagtcaagac tgcatcatgt aaattggcgt ggttgtccaa aaccaaatca gcaaattcaa
    28801 ataatcgcaa accactgcta tgttgcgatt tgatacttcg tgacaaatca aatccagtaa
    28861 caatgattaa tggatgacca ttttctttga tccactgagc tagttcgatg attgccgggt
    28921 ttcttccttc attcgaaaga agaagaaaaa tgtcttctga tttgacagaa agctgatcaa
    28981 taattgcctg tcctgcacct tctagcgttt catagacacc tccggctgga ttttcaatca
    29041 atatacttgg tacaagatcc ggggtttgat cccaaaattc aaatgctaca ccacttagtg
    29101 tgcggctagc atatatctgc aatctaccac cttgattaat ttgtttcgcg atccaagtgc
    29161 cagcttgaag catcagcggc tcttctatcc ttaatttctc ttccaattgg tgataatagt
    29221 caaaataaca taaactcatt tatctctccc gcctagctat acttgacatg aatagaaaca
    29281 gctgcctaat ctaaggaagt acataaaaaa acagtagtta gacagtaaca catgtggtct
    29341 ttgctgtttc aaacacaaaa aaggcattga acgaatacgc ccaatacctt gacatagcaa
    29401 ataaaacaac gtgcagacca tggaaaatat ctacataggg gtagaatttt caccctagat
    29461 taggtacaat aaattaggtc gatgcactgc attgactatg gcaaaatgat cggtcagggt
    29521 gtatgtgtgt gactggtcat tccctgattc ttagcgccaa ctaagggtca gggagccttt
    29581 ttttattcag ctgtttctat ctgaatttat tgtaagtaat ctcttatcca agtgcaagca
    29641 agaaaataca aaaaaacatt ggtatgattt atatatatat aaataacata aaaaaaggaa
    29701 tggcttatcc cgatacaaca agcattcctt taaaaaagat tcttttatat aagtgaaaaa
    29761 agcttttttc caccaattaa ataaatctct catctcaata gtttatccat tattttatct
    29821 ttttttgcgt atacctacta aatattaccg tcttcttctc gttcttctac tttgttcgag
    29881 agaatctgta ctagaaaaag tagttgtatg tctacctttt ctgcctatcc gagactttct
    29941 tttcatatat gggatataaa tgattagata cagtacagca aaaaataaag caaatacgca
    30001 agcttcccaa ctacctaacg ccaattcaac catcaaggta gcaaccccaa cggatgctgc
    30061 ataccagata aaaatcagaa acgtttcttt ttttctgacc atgcgaaaca tgataaatag
    30121 actaataagc aatagtggca acacaaatat ccaattgctt ccttccatca gccacttgag
    30181 tatatcaagc agcgactgtg caacagcgac taattttccc atagtgaaca cacacctttt
    30241 tttataatta aaaaattcgc ctttttattc tactatgaat cttctggctt ggctttcatt
    30301 tctcttatgt aatcattttc ttttatacaa cttaaaaaaa aattaaacca ttagcataat
    30361 attttctgaa tctattcacg aaagtctttt tctatctttc gttttccacg actcaactga
    30421 tctagagtca aaaaactaaa acgacagttg cgcaaatgac gcacaagctg ccgctttaat
    30481 aacaaatagc ctttcattct cagcaggcca cttatttcca gttaactttc tgttcttagc
    30541 tttttactca actggataat atatgcactt aatgaagtcc caatttccga atgttgaagt
    30601 ccatattcaa tatttgtctt catatatcct aacttatcgc ctacgtcgta tctttttcct
    30661 ttaaactctc tcgcatagac cgcctctgtc tggttaagag tctgaagtgc atccgttagc
    30721 tgtatctcgt ttcctgctcc tggttcttga gtttctaata tctcaaatat tttaggagtc
    30781 agtagataac gtccgataat ggctaaatta cttggtgctt ttcccatttc aggtttttct
    30841 acaaaccctt ttactctgca acgacccttt tcgctatctg ttttaggatc aataattccg
    30901 tatttagatg tttcattttc tggtacacgc atgacggcaa cagcggaagc ttgtgtttct
    30961 tcataatcca tcatcaattg ttgagtcaat gggatatcat ctttcatcaa atcatccccc
    31021 agcatgacga caaacggttc atttcctata aatgcttttg cttgaagcac ggcatccccc
    31081 agtcctcttg gatgagattg acggatgaag tgaagattga cttgagtcgt ttcttcaact
    31141 aaagcgagta attctgtttt ttccttttcc cgcaaattgt tttccaattc gatatttgca
    31201 tcaaagtgat cttcaatggg tcttttttct tttcccgtta cgatcaagat gtcttcgatt
    31261 cccgaatcca atgcttcttc cacaataaac tggatcgttg gtttatccac gattggaagc
    31321 atttcttttg ccatggcttt tgttgccggc aaaaaacgtg ttcctagccc agcggcagga
    31381 atgactgctt ttttgacctt cacttgctgt tcctcctaaa cgacgattaa ctattgatca
    31441 tctatttaac gaagagaaaa ttcattctcc atttttccgt cacgtaacat gatttctttt
    31501 gctgcttttt tgatatcttc tccattatac aaaacattat agattgtttg agtaattggc
    31561 atctcaacac ctagctgttg agctagctct actgctgctt ttgtcgttgc cacaccctca
    31621 acgaccattc ccatgttttc tagaacttct tccagcttat gtccctgtcc taacaagtta
    31681 ccggcacgcc aattgcgaga atgaacactt gtacaagtaa cgatcaagtc ccccacacca
    31741 ctcaaaccga taaatgtcag aggattggca cccatcgcga caccaagacg gctgatttct
    31801 gccaatcctc gagtcatgat tgctgctttt gcatcatctc cgaatcctaa tccatggata
    31861 gcgccggcac caattgcaat gatatttttc agtgctgcac cagtttctac gccaatcaca
    31921 tcattatttg tataaatcct gaaataatca ttcatgaaca gtttttgtac ataggctgct
    31981 gctttttgat caatacttgc agctgtaatt gttgtgatat catgtactgc cacttcctca
    32041 gcatggctag gaccagataa tacaacaatt gcctgtcttt tttcttctgg aatttcttca
    32101 gccaaaactt ctgagattcg tttatgcgta tcttgttcta gtcctttact tgcatgtata
    32161 atgattggct tagtattcat tttaccaata agttcctgtg cgactgaacg aatcgctttt
    32221 gttggaacaa caaataaaat agcatctgcg ttatccactg cttctgccaa ttctttatat
    32281 ccgataatcg attctgggat cgtcaaatca ggtaaaaaat gctgatttgt atgatactta
    32341 ttgatttcat caatctgttc aggtacattt ccccaaatac ggacatcatg cccgttttct
    32401 gccaatgttt gtgctaaagc agtgccccaa gatccaggac caagaactgc gattttttgt
    32461 ttcatggata ttcctccaaa taaataattg ataatttcca ttatagtata catttcttaa
    32521 agttgggact aatttccatt gtttttaagt gtttgtttac ttcttttatc gtttttactt
    32581 gaaataatca atgatatgtt acaatagtct acattttatt tttacactcg ttatgctttg
    32641 cttgttccta agaggaagca gttttgacaa tgttactacc agcattacta ttaggaggtt
    32701 aggaaacttc tcctagtgag tattgattta aaatacataa gagacagatt gaacatttct
    32761 aaatctgtca ggtcctgcaa cggacagatt gtctctgttg acggttgaca taggaacttt
    32821 tgttttagtc aacattctgg atacaaaacg ctatgtaaac tggcgcaact cgttgtactt
    32881 ggacttttac tacggatcca tgcaacaact aaatagtact attgcataac aaaaaatggt
    32941 tggcacccac atgtcccatt aaacagatta taagttgaac tgtagataat gcaaaaaatg
    33001 tgttttataa cgtaattgat gccaatattt taaaaaaata aagggagcag ttatcgctgc
    33061 tctctttatt ttttatattc tttttatcca gtctatccta ataatcaaac tgacgatagt
    33121 agcggcgtat ctctaatgga tggcagttca actgctccac atgagcatcg atgcgttgtg
    33181 tcactacatg catcaataag aaagacaaaa cacgtccgcg atatttttca ctgatacctt
    33241 ctactggata gtcttttgtg tctatcaatg tataattgct gcaaattttt ggcaaaagtt
    33301 tagccacacg ttcagataac actctctgtt catcttcgcc taaaaataat gtgactgggg
    33361 ttgtttcttc tacgatctct aacgtcccgt gaagaaactc ggcagcatgg atagatttcg
    33421 atggaagcca gctttgttct tcccagtaac acatggcata tgaataaaca gctccccatt
    33481 gattccctgc tccaataaaa taatgcatac tatcacgtcg atgtttttct gcaaatgata
    33541 agccaaatgc atctgctttt ttctctgctt ccactaaccc tgttggtaaa taacgttcga
    33601 gctgttcata gtattctgaa taagcttcga attctccatt caaatacatc aatcgatctg
    33661 ctgccataaa aaatttgatt tgttctgttc caggagctgg ataagtgacc acaaaatcgc
    33721 atagctgaga caacttactg ttcgctttat cgataaatcc aatcagtgtc gctcctactt
    33781 tcttgatttg ttctactgct ttcactactt cttgagttgt accggtaaca gaggacaaaa
    33841 taacgataga atcttttgtt aatcttttgt ttccagtagt gtaatattct gcagcatgct
    33901 gtacaaagac aggcaagttg cttttcccat tcatataagt gacagcttgc atcgaggaag
    33961 catacgttcc accgattcct agataataga cagcgtcaaa tttttccgca taaagtttat
    34021 cgatgatttc ttctacttgc ggacgtaaag ctagtgcgcc ttttattgct ttgatttgtt
    34081 cttcttcatt gaatttcagc atgatttcca cctcttataa tttacttttc aagactgtat
    34141 caaagtcttc aaaaatcttt ttgttcaagt caataccttt agctactgcg gttttataag
    34201 cgaatagttg aaacggaacg acaagcgcta atacagacag atattcatca cattctattg
    34261 ctaatccaag tgttttctcg tttcttgctt tttttgtggt aatggtgaga gtctctccaa
    34321 cgtaccgact catatatctt tgcaactttt gcgaacgttc cttatttaca ctttcccctt
    34381 caataaagaa tagcaaatgc gaagcatctg cctccaggta tgggccgtgc atataagctt
    34441 ctaactcaaa tccttgtgac ggaacgcgga ttgtttcagt caatttcgtc tctgcttctt
    34501 tagctgttcc ccagtttgga ccatatccaa tggtgacgaa acgctgtgct aaacgaaata
    34561 aactttgata tccatcaaaa aattcttccg ttttttgaat aatcgctggt aaatggttga
    34621 ttattttgcg caactgttgc atgtagtctt gttctatttt tttgctgatt ttatttttac
    34681 ttattccaat tcctatccct aagagtagta actgtaaaac agtcgcagaa tagccttttg
    34741 taacgaagcc tactgattct accccggcac ctaaatcaat aatttgtgca gcttctaaag
    34801 caagtggact ttgcacgtca tttgtgatag caacaacagg taacccttgt ttttgtatca
    34861 tttttactgc ttcaacagta gaagcacttt tcccgctttg tgacacagca atcacagtat
    34921 cgattgaagg agaaagtttt ccataattga aaaaataaaa aggttcttga atatcgacag
    34981 taagatcacc ataagattcc aatgctggct tcgctgctaa acaagcgtta taagatgaac
    35041 ccgttgctaa gatcagtaaa ttcactgttt ttttcatatg ttctcttgtc gaaaaatcat
    35101 tctttttcaa gatcgtactt agtacttcct tttcttcata gatataatct tgcatcgtaa
    35161 acattttctc tgctcctttt ttaaaaaatc cctatccacg aaccaaaaat tccaaagaaa
    35221 gctagtccga tcaagatcgt taatggtttc acttctttct tcaatagata aaacactatg
    35281 ccaaatgctc caagacataa gatatttgga acgatatcat caaaaatact ttgaacagtc
    35341 acagcatttt ctcctgaacc gatttttaaa ggaatattga tggtgatcat cgtggcagtc
    35401 attgctccaa cgaccatcag tccaataata gacgcgccta atgtcaaact ttccatcata
    35461 ccgtttgcct ggatcttttt caaaaaaccc gttcctaagc gatatcccca acctgttgct
    35521 agatagcgaa acagaatgtg cggaatattg aaaatcaaaa tgaacaagat agggcctaaa
    35581 atatttcctt gcatggctaa agaggtaccc acacctgtgg caatcaatcg caaagttccc
    35641 caaaagaaag aatctcctaa ccctgcaaga ggtcccatca aagatgtttt gatactatcg
    35701 atcgcagtca catcaaattc aggatcatcc ttattttctt cctccattgc agccgtgatt
    35761 cctaaaatca gtgtaacgat atgtggggtc gtgttaaaaa actctagatg ccgtttcaaa
    35821 gctgctgcca tttcttcttt cgactgatat aactttttca aaatcggaat cattgcataa
    35881 acataagcca tattcatctg acgttcataa ttccaagaaa actccatttg aaaagaacgc
    35941 caaaatacgt gatttagctc ttttttcgtt attttgctgt ttttgatctc agaattcttc
    36001 atcttcttct acctcccctg cagttgtttg aagtggtagt tgacggacat tcataatatt
    36061 gactaccaca actgctaaaa tcgcaccaaa aatagctact ccggtcacag gaatctctaa
    36121 gtaagaagct atagcaaaac ctaagaaaaa atatggtgca acttgtttgt tgatcagcaa
    36181 acgagcaagc atcgcaaaac caagagctgg aatcaaccct gtagctactg acaacccctt
    36241 ttgaacgaag tcaggaatca tatttaatag atttccgatc gtattgcttc ccaccatata
    36301 agaaaccatt accacaacac caagcatcaa ggaaagaccg ataccggcaa gcaagtgcat
    36361 atgctcgact cctttataat ttccttcttc tgcataattg tctgctttat ggttcataat
    36421 tggaatcaaa agccctaagt aaatattttt caatatcaat gtcaacgtag caatcggcaa
    36481 acctagtaaa agcgcggttt ctgtaccggc tccagctgta atagcaaaag ccgtccctaa
    36541 aatcccaccg gtcacaacat caggtggaat cgaagcacct actgagaacg atccgataaa
    36601 tgctaactct aaagttgcgc ccataatgat cccagttttt acatcaccta acacgattcc
    36661 tgtaaacagc cccgtcacaa tcggacgaga aagcaacgat gtccctagtg catattctga
    36721 ttgtgcgaca aatgcaacaa ttcctaataa gactgcttgt acaatcattg aggttcttcc
    36781 tttcttacaa aacttctctt acatccactt ttttatctgc tggtacttga cggatttcta
    36841 cttctatttg tttgtcaatt aatcgtttca agcgttcctc ctcttctggt aaaatattta
    36901 ccgcttttcc gatgctgcga gtaccaggtt ttgccttgat ccctcctaag ttgatggttt
    36961 tgatttctgg atacgcttct gccagcttat atgcatcctc gatcgattcc actacgatga
    37021 ataacttata tttatctgtc accccgcttt tcaacgcggc aatcgagtca tcaattgatt
    37081 tgatcactag tttgactcct tgtggcttgg ctaatttgat cgttgtcttt cgtatctcat
    37141 tcgtaggtac atcatcatta gcaatcaaaa tacagtctgc gcctattgtt tgtgtccatg
    37201 aaaaagctac ttgtccgtgt aataaccgat ggtcgactct tgtcaataca atcatttttc
    37261 ttcctcctaa aattcgtctt cttctatttt ctgattgagc cgctcgttac aaaactggat
    37321 catttttttt gaatcagtta gcacctgctg gatcaaaaca gttgtctcta cttcagcgtc
    37381 caaccgagat actagctcaa tcaatagtgg caaattcaat ccggcaacga gatagacatg
    37441 atcccgattg atatattgta agaattcgtt gttgacactt ccgccaaaaa gatccgtcag
    37501 aacaatgatt tctttatctt tgtacctcat aaaaagctgc cttactgttt ccgctaaatc
    37561 aaaattctca tcagtatagc aatcgaatac agtaatagaa tgatttttcc catagatcag
    37621 ctctaatgaa tctaatatcc ctgaagcaaa ttttccatgg gaagctaaga taatttctct
    37681 atccactttt tcatctcctc ttttcactta cacatactaa aaaagcaagt tgcatgccaa
    37741 aattagtggc aacaacttgc ttatgttcaa aattcttctt ctatcgagag aaactctgta
    37801 tcttcactca aaatatcata gatataggca atctcagaat tggggatcat gacactataa
    37861 tgctcttcaa tgacactaaa cgcagattta atagcagata acccttcttt ttgacactgc
    37921 ttccatttct ctaatccttg ataggtttcg attggcattt gtcggatcaa tcgttcaatg
    37981 agacagctca catggacata caacgctaat tttttgggat tgctgatgct tcgattcaac
    38041 tgcacctcta attctctcat aaaatgatcg atttctccca tcaccttctc tgtatctaga
    38101 atagtcacgg aatccagtac tttttccaat gaaaaattac gaatgatgcg ctgattgaat
    38161 atttccttct ctgaagcatt catagtagtg tccaaccatt cagctagtac agacatcttt
    38221 tcccccgaga tgaggtcttc taatgaaaga tagtcgatat tggtgatatc aggatctgtg
    38281 gtgccaatca ttcctacgac ttcgtatatg gaaaataatg ggtcactgtt tttcttatct
    38341 tttaattgct gatattcata tgggatgatt ttcaactgac aagccgtagg taagcttttt
    38401 tctagcagtt cacttaagtg gaccgccgta ccgattccag tgaagcaagt agtaatgagt
    38461 gctttgggtt tgttttcctc tggatagagc agtcgccatt ctggctggat ctgtcgaaca
    38521 gtctcttctg ctatctcttc taatgataca tttttttgca ggcattcccc tactgtgatt
    38581 gctaaaggtg tcgtcacatt attcaaaatc aaaagcggta cggataactg ttttttaaaa
    38641 aactgatgga tttctttcag agatcccatg tctactaata tgatcaatcc atttgaaaca
    38701 tcgctgtttt cgcaataatc aaggatttct gaagcaatct gctgtggtgt gacatctagt
    38761 ggcatatcaa atgattcaaa tagatcctct tgtaataaac gatttactac atttgccata
    38821 ctgctagcag ttgcataacc gtgagcagca atcatcgctt ttggcagtcg tttttctttt
    38881 gaccatttcg ctttttttag atagatcgtc agtaaaatcc gatccatact tgtgtcttca
    38941 atatctaatt tgatcttact taattccaga attcgttgga catagtgata actagatgga
    39001 taagactgag aaacttgttg ttctaattct ttgattagtt ggataatatc atgttcttcc
    39061 ggtaaccatt tgatagtgct tcgctgatag agatagtaac tcaaggcata gacactgttt
    39121 ccatcgaatt tgatttgata ggcagtttcc atttgtcgaa aggtctctcg gatagattgt
    39181 gtccaataaa ccagcagctc gtgtttttct tgtctactgg tctcaaacag caaaaaatca
    39241 aataattgaa tcacttcttg ctttaatttt ccttcgcaag aatatagcag acagttattt
    39301 ttcttgaaat caattaggat ttgctcaaaa cttttaacga ttcgctgctg ttcgggctgt
    39361 tgcttctcca ttaattgctc taaggtctgg tcttgcgtaa tatagattcc ttgctgtgct
    39421 gtatttttag taataggctg cgcaagtagt tctttttgca aatgatggat cgtgacatgg
    39481 atttcaggct gttcacgctg ttcagcatat gcttttgctg ctgaaacttt gacgatattt
    39541 ttcagctctc ctatgtttcc ggataatttc tgacttgtta aaagtgacaa agctggtcca
    39601 ctgactgaca aagggctccc catttttctt tgttcttcga gaaaaaaaga gtaaatcagt
    39661 tctagccgtt cattacgact acgctgatta agtgcaggta gttcgatacg gataggtatt
    39721 cttcgaatga aagttgttaa gaaatgacta gtaatctctt cagtcgtagc aaaaaagaga
    39781 cgtgttttga tccggcgtga caaattaggt tctcccatac gataaatcac accttgatca
    39841 agataggtga acagtttttc ctgaccttct ggtgttaagc gatgtacctc gtctaaaaac
    39901 aagatcccac catctgctgc ttcaaaagcc ccttttttat tttcttctgc accagtgtac
    39961 gctcccttaa catgtccaaa caaattgcta gtcaaaagtt caggattatc tgcatattgt
    40021 gcacaattaa aagtgatgaa tggtgcagaa tcttctagca gatcatgaag cagacaatac
    40081 tgatagacca aattaactaa ataactttta cctgtgccac tttcgcctgt aatcaaaagt
    40141 ggtaatccgc catctggata gtaaagagct gttttgattt gttcgatact ttctttcaaa
    40201 ctggcgtcgt atccgatcat aaaagagaat aagtcttttg gttgcgcgaa aaaaggctgt
    40261 tcctctttga gatcatcgat agaaaaataa aaattcgtgc gtaacttaaa aaattgtttt
    40321 tcaaaagctt ctttgtggaa ataaatcact ggtcgagaat tgattttgac tagtagttgc
    40381 tcatcattta actggttcaa ataatgactg actgtgtttc tttttacccc aaaatgatca
    40441 gctaaccttt gtgctgtaaa gacctcgttc atatgttcta aatcgaaaaa ggctgtctgg
    40501 ttctctaagt aacataaaat ttccttcttc aacaacttat tcacccctcc tccatcatag
    40561 aaaacggatt cattttgtca ataaaatctt taataaagaa ttgaaaaata tatcttatta
    40621 tagacagcag cctacttatc ccttataatt tttattctaa aaaacgtaca gttccttcca
    40681 gtttttatac tggaatttca acatatgaaa gaataaggag gctatgacta ttcaatgaaa
    40741 tcggataaat ataaaaacag gaaacatact tcctggaaaa cttttcgaga aatcatcgga
    40801 tcttatctcg ctgtagttgt atttttgcct ttgctttata tggctatgac agaacaaact
    40861 cgttttattg gcgtagatac gcaatatata gccgaaacag gatttttatt catctttatc
    40921 ttgatttttg cgttctataa atccttccat acaaaataag attcacagat ttcaactttc
    40981 ctgtctgttt ttagctatta caaatcctta tatgtggtat aacaacaatt tattggcata
    41041 atgaggaaaa acgagttatg atgctttcag agagtaaaga ggtactgttg tcaccatcct
    41101 ttttattgat tacgtacacc actcttagtt ttttcccatt gattacgaaa acaaggtaga
    41161 aacattgatg ttcctacctt gttttctttt tttattttca gatcaaaatc tcaagtagct
    41221 tttgacttga tgtcgggagg ttgtcttcgt tccatcaaac ctgatagaat ctgcgccaaa
    41281 ccatagtttt ctccactatt atttattcct ctaagatagt acccacaata aacgaataaa
    41341 tatttttctc attttcatac tccttctttg aaaatcaatt agatttctct caacccactt
    41401 ttttacaaaa attacgtagc aaaaaagatt gcaagaacaa attaaaaaag aagtaaatag
    41461 agtttcatct ataaacattt acttgatata ttttttatgc agaattcatt tatagaaaaa
    41521 ttaagtaaaa tagacgttcc tatgaatgaa gagactggca tgttttctca ttttcttttt
    41581 tttaaaaaaa atagtctatt caatttctaa agcttcttca taataaaact ttcatttatg
    41641 aaaaggcttg ttctcttttc attctaaatt tcctaagcta caatgtggta gtacttgttt
    41701 tttcaattgt ggacaaataa ataataggag gaatcatatg attcagttat tacgttatgc
    41761 aaaagactat cgtaagcaaa tcattttggg cccgttcttc aaatttcttg aagctgtctt
    41821 cgaacttgtc ctgcctttaa tgatggcttc cttgatcgat aatggactaa aaatgaatga
    41881 ccgaggaaaa atcattgaaa tgggcttatg gatggtcgct atgtctgtga ttggtctgat
    41941 ttgtgctatc atttgccaat actatgcttc agtagcttca caaggatttg gtacagagtt
    42001 acgtaatcag ctaatcaaaa aaatcaatac tttctctcac aacgagttga atcattttgg
    42061 aacagatacc ttgatcaccc gaatgacaaa cgacatcaat cagcttcagc tagctttagc
    42121 aatggtcatt cgtttattga tcagagcacc gtttttaagt atcgggtcag tggtcatggc
    42181 ttttgtgatc gactgggaag taggtctgtt tttcttagca ttattgccta ttttctcaat
    42241 cattttattt tttatcatca aaaaaaccgt tcctctctac caaaaagtgc aagaaaaact
    42301 agatcagctc aatgaacacg ttagtcagaa tcttagcggt gttcgtgtca tccgcgcatt
    42361 tgcgcagaca aaaaaagaaa cagagaagtt cgataaaagc acaaatgatc tagcgaacaa
    42421 ttatttacgt gtttccaata tctctgccct attatcacct gctacaacct tgatcatgaa
    42481 tgtcgggatc atctgtttgc tgacagttgg cgggatcaaa gtaaatattg gtagcttgca
    42541 acaaggacaa gttcttgctt tgatcaatta tatgaatcaa atgcttttgg cactgatcgt
    42601 tgtttcaaac ctcgtcgtga tcttcacacg tgccgaagct tcaggaaatc gggtgaaaga
    42661 ggtgctagac acagaaaatt cgattcttga agctgaggtc agtactactc ccgatttttc
    42721 ttctgaagca attatcgctt ttgaccatgt tgacttccgt tatactcctg attctggtct
    42781 ttctctccaa aatattacat ttcagctgaa gcgaaataca gtccttggaa tcattggtcc
    42841 aaccggaagc gggaaaacga ctttgacaca attgattccg cgtttctatg atgtcagttc
    42901 cggtacagtt ataatggacg gaatggatgt tcgttcttgg cagctggatc atctccgtca
    42961 gcaaattgct cttgttcccc aaacttctgt actatttacc gggactattc gtgaaaacct
    43021 tcaatggggt aagaaagacg ccacagaaaa agaatgttgg gaagctctga aaatcgctca
    43081 ggcagaagaa tttgtccgtc agcttccaaa tggtttagat acaaaagtca tggaaaatgg
    43141 gaaaaatttc tctggcggac aaaaacagcg actgacgatt gcaagagctt tgattgccaa
    43201 gccagcatta ctgattttag atgactcttt aagtgcgttg gattatcaaa cagacttgga
    43261 tttgcgtaag gcgctacaga cttatcttgg cagtacagtg attattatct cccaacgtgt
    43321 ccgctctatt caagaagcac atcatatctt agtgatggat caaggtaaga ttgctgctca
    43381 gggtacccat gaagaattac ttgctcaatc agcagaatat caggaaatcg tcgcatcaca
    43441 ggaggaagaa tagttatgaa agtaaaaaaa ttatccttgc agacgctccg ccgattcatt
    43501 ccttacctta ctgcctatgc aaaagacatt tggctagctg tgatactagg aatcatcaat
    43561 gggattgcaa cagttgtgat gacgttacaa attggtcaaa gtatcgatca aatgattgga
    43621 gaaggacagg tccgcttcaa tcgactgttc catttactta ttctattcgg tggtatcgtt
    43681 ttaatcaacg tagtcagtca atggctcatc caagtgttaa gcaatcggtt agcttactta
    43741 tccgttgcaa aattaagaaa agatgcattt gctcatctga atcgattgcc acttcgttat
    43801 tatgatcaaa actcccacgg aaatatcgtc agtcgattta caaatgatat ggacaatatt
    43861 tccattgcaa tttctgcggc cttcaatcaa ttattttctg gagtggcaat catcattctt
    43921 tcgtttattt ttatgatgcg tctaagtcca cttctgacat tggtcgttct ttgttctaca
    43981 ccaattattt tccttgtgag ttggatcgtt gctcgtgctt cacaaacaaa tttcgataga
    44041 cagcaaagga ttattgggga tatctcgggc tttgtgaccg aacgaatcgg taaccagaaa
    44101 atcgtgaaag cttttcagca agaagaagct agtcaacagc aatttgaact gctaaatgct
    44161 gatctatatg aaaaaggaca acgggcacaa ttttcttctt ccttgacaaa cccgtcttct
    44221 cgttttgtag atcaccttgc ttatttatcg attggtttag ttggcggcct attggcattg
    44281 aagagtggtt cgtcaatcac tgttggtgtg atttccagct ttacgatcta ctcaagtcag
    44341 ttttctaaac catttatcga actttctgga atcacgacac aactgcaaac cgctttttct
    44401 ggtctagaac gagcatttga tttgatcgat cagccagaag aaaagcctga tttgccagat
    44461 gcctatgcct tggaacccaa aaatatcaaa ggaaaagtct cttttgatca tgtttctttt
    44521 tcttatgacc ccaagcagcg tttgatcgaa gatttcaatt ttactgctta tccaggtcaa
    44581 accattgcta tagtaggaaa aacaggggcc ggaaaatcca cacttgttaa tttgctgatg
    44641 cgtttttatg acacaacaaa aggaacaatt acagtcgatg accatgacat ccgctctatc
    44701 caacgtgata cattacgtaa aagttttgga atggtgttac aagatacttg gctatttgac
    44761 agtacattgc gagaaaatct tcgttttggg aatccggaag caagtgacgc agaaatcaat
    44821 gatgccctaa aagcctctta tatgtatgaa tttgtagaac gtttgccgga aaaattagat
    44881 actaagattg gcggtcaagg gttaaagata tcagatggac aacgccaact gttgacgatt
    44941 gctcgaacga tgatcagtaa tccccctatg ttgatcttag atgaagctac cagctcagtc
    45001 gatacattga cagagaaaaa aatccaacaa gccttcttag caatgatgga aggaaaaacc
    45061 agttttgtga ttgcccatcg cctgtccaca atcaaaaatg cggaccagat attagtgatg
    45121 gatcagggag ctattgtcga aatgggcacc catgatgagc tgcttcaaaa agatggttat
    45181 taccgacggc tctatcaagc acaattcaca gcacaataaa aacaagcagt cttctgggat
    45241 gggaagactg ct
//
LOCUS       DACDIH010000017        45050 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDIH010000017 DACDIH010000000
VERSION     DACDIH010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.017"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(665..1246)
                     /locus_tag="IV281_000962"
     CDS             complement(665..1246)
                     /locus_tag="IV281_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8779994.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(1429..2055)
                     /locus_tag="IV281_000963"
     CDS             complement(1429..2055)
                     /locus_tag="IV281_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8779995.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(2077..3408)
                     /locus_tag="IV281_000964"
     CDS             complement(2077..3408)
                     /locus_tag="IV281_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP8779996.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(3563..3964)
                     /locus_tag="IV281_000965"
     CDS             complement(3563..3964)
                     /locus_tag="IV281_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8779997.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(4050..4256)
                     /locus_tag="IV281_000966"
     CDS             complement(4050..4256)
                     /locus_tag="IV281_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8779998.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(4351..5853)
                     /locus_tag="IV281_000967"
     CDS             complement(4351..5853)
                     /locus_tag="IV281_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP8779999.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5871..6326)
                     /gene="argR"
                     /locus_tag="IV281_000968"
     CDS             complement(5871..6326)
                     /gene="argR"
                     /locus_tag="IV281_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8780000.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(6424..7041)
                     /locus_tag="IV281_000969"
     CDS             complement(6424..7041)
                     /locus_tag="IV281_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780001.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            7262..8635
                     /locus_tag="IV281_000970"
     CDS             7262..8635
                     /locus_tag="IV281_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8780002.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8710..9822
                     /locus_tag="IV281_000971"
     CDS             8710..9822
                     /locus_tag="IV281_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP8780003.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9894..11531)
                     /locus_tag="IV281_000972"
     CDS             complement(9894..11531)
                     /locus_tag="IV281_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP8780004.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11699..12307)
                     /locus_tag="IV281_000973"
     CDS             complement(11699..12307)
                     /locus_tag="IV281_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8780005.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12691..13842)
                     /gene="queG"
                     /locus_tag="IV281_000974"
     CDS             complement(12691..13842)
                     /gene="queG"
                     /locus_tag="IV281_000974"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP8780006.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(13861..14613)
                     /locus_tag="IV281_000975"
     CDS             complement(13861..14613)
                     /locus_tag="IV281_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780007.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14754..15704)
                     /locus_tag="IV281_000976"
     CDS             complement(14754..15704)
                     /locus_tag="IV281_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8780008.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15705..16544)
                     /locus_tag="IV281_000977"
     CDS             complement(15705..16544)
                     /locus_tag="IV281_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8780009.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(16541..17287)
                     /locus_tag="IV281_000978"
     CDS             complement(16541..17287)
                     /locus_tag="IV281_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8780010.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            17525..18895
                     /locus_tag="IV281_000979"
     CDS             17525..18895
                     /locus_tag="IV281_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8780011.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18950..19846)
                     /locus_tag="IV281_000980"
     CDS             complement(18950..19846)
                     /locus_tag="IV281_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8780012.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(20127..20804)
                     /locus_tag="IV281_000981"
     CDS             complement(20127..20804)
                     /locus_tag="IV281_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8780013.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20895..21800)
                     /locus_tag="IV281_000982"
     CDS             complement(20895..21800)
                     /locus_tag="IV281_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8780014.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTGESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            21975..23429
                     /locus_tag="IV281_000983"
     CDS             21975..23429
                     /locus_tag="IV281_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8780015.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPTLSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVASASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            23445..24476
                     /locus_tag="IV281_000984"
     CDS             23445..24476
                     /locus_tag="IV281_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8780016.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            24483..25670
                     /locus_tag="IV281_000985"
     CDS             24483..25670
                     /locus_tag="IV281_000985"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP8780017.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            25826..27208
                     /locus_tag="IV281_000986"
     CDS             25826..27208
                     /locus_tag="IV281_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP8780018.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            27180..27566
                     /locus_tag="IV281_000987"
     CDS             27180..27566
                     /locus_tag="IV281_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780019.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(27766..28434)
                     /locus_tag="IV281_000988"
     CDS             complement(27766..28434)
                     /locus_tag="IV281_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP8780020.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(28623..29420)
                     /locus_tag="IV281_000989"
                     /pseudo
     CDS             complement(28623..29420)
                     /locus_tag="IV281_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(29701..31080)
                     /locus_tag="IV281_000990"
     CDS             complement(29701..31080)
                     /locus_tag="IV281_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8780021.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            complement(31255..32424)
                     /locus_tag="IV281_000991"
     CDS             complement(31255..32424)
                     /locus_tag="IV281_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8780022.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            complement(32399..33550)
                     /locus_tag="IV281_000992"
     CDS             complement(32399..33550)
                     /locus_tag="IV281_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP8780023.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            complement(33510..34871)
                     /locus_tag="IV281_000993"
     CDS             complement(33510..34871)
                     /locus_tag="IV281_000993"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP8780024.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            complement(34868..35869)
                     /locus_tag="IV281_000994"
     CDS             complement(34868..35869)
                     /locus_tag="IV281_000994"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP8780025.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            complement(35885..36580)
                     /gene="dapD"
                     /locus_tag="IV281_000995"
     CDS             complement(35885..36580)
                     /gene="dapD"
                     /locus_tag="IV281_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP8780026.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            complement(36586..37302)
                     /locus_tag="IV281_000996"
     CDS             complement(36586..37302)
                     /locus_tag="IV281_000996"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP8780027.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            complement(37299..38180)
                     /locus_tag="IV281_000997"
     CDS             complement(37299..38180)
                     /locus_tag="IV281_000997"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP8780028.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            complement(38183..38983)
                     /gene="dapF"
                     /locus_tag="IV281_000998"
     CDS             complement(38183..38983)
                     /gene="dapF"
                     /locus_tag="IV281_000998"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP8780029.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            complement(38970..40229)
                     /gene="lysA"
                     /locus_tag="IV281_000999"
     CDS             complement(38970..40229)
                     /gene="lysA"
                     /locus_tag="IV281_000999"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP8780030.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            complement(40715..41278)
                     /gene="efp"
                     /locus_tag="IV281_001000"
     CDS             complement(40715..41278)
                     /gene="efp"
                     /locus_tag="IV281_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP8780031.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            41706..42623
                     /locus_tag="IV281_001001"
     CDS             41706..42623
                     /locus_tag="IV281_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP8780032.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            42636..43781
                     /locus_tag="IV281_001002"
     CDS             42636..43781
                     /locus_tag="IV281_001002"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP8780033.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKRDEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            43952..44410
                     /locus_tag="IV281_001003"
     CDS             43952..44410
                     /locus_tag="IV281_001003"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP8780034.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            44569..45039
                     /locus_tag="IV281_001004"
     CDS             44569..45039
                     /locus_tag="IV281_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP8780035.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT        12650 a         9351 c         8146 g        14903 t
ORIGIN      
        1 tcaatttttg tagaactaaa aaagttgtga caagtgacaa actctccagt gaatggtatt
       61 ctcctgccaa ctcctcggtc acaagtcaca atattcaaca aacatgagaa gctgcttttt
      121 gaaattgttc cttgtgactc ggagtgaaac ggcaggctta cgacctctgg gtaaacggtg
      181 ttcaaaagcc agtttctcgg tattaagcca aatttatgca aaaatgtaga aagctatttt
      241 cacaaaattt ttcttaatac tcgaaactaa gcgacttttt cacaacctct tccttaaacg
      301 gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata tgagcagcta
      361 tttttatttg gctgttctta ttacttgatg cagggcagct ttttcacaac ctctccacta
      421 aacgatgttc aatcagctaa ccttcggtat tagctcaaaa aacggcaaaa tatgaggaac
      481 tattttgccg tttgtttctc taatactcag gtcaaagcgc tgatttcaca acctctaata
      541 cacggtgttc cagaagtagc ttcttcgaaa aataagccga aatttcacaa aaatttgaga
      601 agcaattttc gtaaattcct tcttatttct tgaagctgac cacttctgtc acaacctcta
      661 attattaaag aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc
      721 taagtagtga tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa
      781 tcaacgcttt ggcttgacgg tctgcttctt catcggacag tttttcttca gaaagtgcca
      841 ggatattttg tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt
      901 attcttcatc cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag
      961 tgatctgtgt ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg
     1021 gctgttttcc agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg
     1081 catggatcac cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt
     1141 ttcctgtatc agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat
     1201 aaataagatc tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt
     1261 ttcatttctc atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt
     1321 aaaaaagact gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc
     1381 tagatgttca ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg
     1441 ctctaatact tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc
     1501 tgcatccatt gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa
     1561 tccactctct accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc
     1621 aagaattact gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg
     1681 tccaaagatt ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc
     1741 ttcgataaaa tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa
     1801 catctgctgc tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg
     1861 tgggtaaaaa taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac
     1921 catttcaatc atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa
     1981 gccaccgcct gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt
     2041 tttattattg aacatggtgg attctccatt cgttatttat tttaatacat catttagagc
     2101 ttcactcatt gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc
     2161 attcatagct agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc
     2221 ccctagaatc aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc
     2281 taatacttgt gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc
     2341 ttgtgctttg gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac
     2401 actgtaaggc acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc
     2461 cataatgatt cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc
     2521 tccaatcgcc caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc
     2581 gcgatctgtt agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct
     2641 tccagttgca gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa
     2701 ggtgacggca gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa
     2761 ttcgattccc gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg
     2821 tggaagaaat tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat
     2881 tgatgcaaat tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg
     2941 ttcctgatcc atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat
     3001 tggtaaaatg attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac
     3061 tttttcacca ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc
     3121 catcactgtc ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc
     3181 cgtcaatttt tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa
     3241 aatcaatgat ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt
     3301 tgacttttca acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt
     3361 accgcccttt ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct
     3421 tcttttttac ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa
     3481 tcatctgctt tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc
     3541 tggttcgatt tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag
     3601 ttgctttgga gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt
     3661 cgctgatgcc atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta
     3721 aattataaag agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga
     3781 tcatatgttg taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg
     3841 gatttgttcc ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg
     3901 gtgcctcgac ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt
     3961 tcattgtact gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac
     4021 aagtaaggcg ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact
     4081 atttttccaa aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa
     4141 taaatagcaa tcttttctac ataatctttt atatgattga ttttaagata catatatacc
     4201 agaaagttga ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt
     4261 tcctcctttt gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt
     4321 ccaagcaact agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga
     4381 aaaatgtccg aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc
     4441 attttctatc tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc
     4501 gtttccgtat ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt
     4561 catccctttt tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc
     4621 gcgtggttca tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa
     4681 ctctttgatt tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag
     4741 gatctttgcg atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg
     4801 tccgcttgtt tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt
     4861 attgattaca tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat
     4921 gaatctttgt agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc
     4981 ttttaatacg attgtactta atactgcgat caaattattt cgattgatca ctgttccaga
     5041 agcactgaca actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc
     5101 atttgctaaa acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat
     5161 atgattagga aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa
     5221 gctattcaat actttttcag caaagaatcc accagcacca tttccggcat caacaatgat
     5281 tttccaacca tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac
     5341 taaatcttgc gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct
     5401 ttttattttt tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc
     5461 tgaggtaaag aattttattc cattataata atagggaaga tggctagcag tcagcataac
     5521 cgcagcatcg cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc
     5581 taattcgaaa tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt
     5641 cagtgctggt ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg
     5701 atcggttgat ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac
     5761 catgtctgtc gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc
     5821 cgatccattt tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc
     5881 attttttcca gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg
     5941 aatgtatcta caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa
     6001 ttcgttacca catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac
     6061 tggatacgca aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca
     6121 gatggctggt taaataatgt atagcgtact attttgtttt cattttgact ttttactaac
     6181 ttcatttctc gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc
     6241 agccgcgcaa gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc
     6301 agtgcttgtc gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag
     6361 ataagtaata tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg
     6421 ttgctaacct ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt
     6481 tcttggatcg ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt
     6541 tgcgatgaat gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg
     6601 taagatttgg gcacaaagac cttcttccat ctttttgaat actacttgtt ctttcaagtc
     6661 ttccggaagt ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc
     6721 aggctgtcgg atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa
     6781 atacttttct tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc
     6841 aggaatcagc caattttttt taggcgacat gcgtattgca tagctgactg catatagaca
     6901 ggctactctt cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa
     6961 tgatttttta ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt
     7021 acgccattca attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat
     7081 catacctctt tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag
     7141 aataagtgaa gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg
     7201 tttctatttt tataatagaa atcactctct ttttttacga aattttttag aggtgatgta
     7261 aatgacaaaa caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc
     7321 ttttgcgggc gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca
     7381 gtttgattta acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc
     7441 gatcattgtt ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt
     7501 agcttcaaat ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat
     7561 ggtcctattg tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat
     7621 gttccatatt atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg
     7681 tactttgaca acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc
     7741 acattttaca tggaatcata tcttcctgtt tcttgtgccc gttttgattc tttctttgat
     7801 tatcggctgg tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat
     7861 aagcgtttta ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg
     7921 aacagtttcc agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta
     7981 tcgaagtaaa aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt
     8041 ccgcttgttt ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt
     8101 cttgccgaat ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat
     8161 gcttcccggc gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca
     8221 atatggtccg aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc
     8281 attattccta ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta
     8341 tatgatcggt atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa
     8401 tgaagaacta caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc
     8461 tgtggctact tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa
     8521 ctatgaagtg tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct
     8581 tctcgtaagc tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc
     8641 atatacggca ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga
     8701 ggctttttta tggaaaaaat tgccattatc ggtggcggga tcattggtat gaccctagcc
     8761 agttacttag atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca
     8821 actaaagcta gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg
     8881 tatcgtctgg ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt
     8941 gatcaaacga tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct
     9001 gaattggctg ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa
     9061 tggctttcac ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg
     9121 cgtatcagtg gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt
     9181 ttagcacaaa aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat
     9241 tcttggctca tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct
     9301 ggcccggcac ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa
     9361 aaagggcagc tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct
     9421 atgcttgacg gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct
     9481 acccatgaaa acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg
     9541 actaagagta cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag
     9601 ttccgtgtag gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca
     9661 gatgatccaa gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct
     9721 tatatcggct atctattagc caacttctta aatactggtc attggcctac ggaagattat
     9781 caaaaggaca tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag
     9841 taaggatccc gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc
     9901 aatttctgaa tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct
     9961 atctgagctt caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag
    10021 gacgtatcct caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat
    10081 ggaccaaacg acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc
    10141 gtttcaggtt tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt
    10201 tgctgtttca aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg
    10261 aaaggattag caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt
    10321 tccgcgtctg atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca
    10381 ttgatcttga taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc
    10441 ttttcgctaa gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca
    10501 tttccgtttc gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg
    10561 cccacattct gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt
    10621 gttttatcaa agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg
    10681 aaagcatcgt catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc
    10741 gttgattttc cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta
    10801 gcatattgtt tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa
    10861 gcaagcgtca aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacagccgct
    10921 gtattatgga gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg
    10981 ggatcacaaa agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct
    11041 aagatcttcg aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat
    11101 ccttctggaa tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact
    11161 tttgtatggt aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg
    11221 tatttgcttt gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc
    11281 acaatgcttc catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca
    11341 gcatgtgaga ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa
    11401 gctgtcttcc tatcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc
    11461 gatcgaatac tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct
    11521 tttgtactca tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt
    11581 attcgagttt ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa
    11641 aaaaaccgag aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt
    11701 agtaccagcc gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg
    11761 ttacatattg ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg
    11821 aagcatctaa ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg
    11881 attctttttg tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg
    11941 gggcagcagg agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg
    12001 ctgtttcagt tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat
    12061 catttgaagt tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg
    12121 aatttttttc agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct
    12181 tagataatga atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc
    12241 ctacaaacat tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg
    12301 aattcatatt aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat
    12361 catttagaaa tatttcatag gtaaaagaaa catgacattt aatgacaaaa taacgaatta
    12421 tttaatattt gtaacattta aacaaatatt tttttgatac aaaaccataa aagccttgtt
    12481 gtataagcaa agtaatcgtt tttctatgtt tttttatttt tgaatgaaaa acgcagattc
    12541 tgtaacattc actgcaataa tccataaaaa aaagcaaaaa aatttttgat ttttatcaaa
    12601 ttctcttaaa aaaatgtaaa aaatacaaaa aaaccagtaa ttgttacaac aattactgga
    12661 caaaaacttt gaataatgtt aactattttt ttattttaac tttctccgca ataatttctg
    12721 ggcatgttcc aattcgttta taacgacttc ttctgtttct ttagctaata tttcatctaa
    12781 tacatcaagc catttttccg gttcttttgt tcctagacgt ccaatagccc aagctgctgt
    12841 tcctctgatg acagggcgga catctgttaa acattctgaa atctcaggca atgccgttcg
    12901 atcacctaga ttagctaaag caatcaatgc gtttctctgc aaaggtttct tcccacgcca
    12961 tgaaccagct aaatgcccaa attgctgttt gaagtcttta ttagacatcg aaagcattgg
    13021 ctttaatttt ggatatattt cgtctacttt tggttccatc tcttcatgaa aatgaaaatc
    13081 ttttccttga ttgtaagggc agactaactg gcagatatca catccataaa tgacatttcg
    13141 cattttttta cgatattctt ccggcatcat cccttttgtc tgagtttgat aagacaaaca
    13201 tcgctgggca ttcattcttc cgtcaccaag caatgctttt gtcggacaag cagtcacgca
    13261 gcgcatacag tcaccgcaac caaattctcc tggtttatca ggctcaaata caatattagt
    13321 aacgatctca ccaagataga caaatgaacc gaactcttca gtgatcaaca atccgtttcg
    13381 tccaataaat cctaaaccag ctcgctgtgc tacagcgaca tcgatcagtt ctccagtatc
    13441 tacttgcgga gctagacgcc attcctcttc tttttgccat ttttctgctc ttgtttggat
    13501 gaattcgatc aatcggttca aacggtctct gagtatatca tggtaatcga tcccccaaga
    13561 agcacgagca aactgcccgc gtttttcatc cctaggcact tcttcatgga tcctagtagg
    13621 atacgcaagt gcaatggaaa tgatcgattt cggctgatca aatgtcattt ctggataaag
    13681 acgctcatcg atatttggat gttcaaagcc agaagtatgg ccagctgctt tttgttcttc
    13741 caacgattct tttaatgaat caaaaggttc cgccgtggtg aacccgattt tgtcaattcc
    13801 taatcgtttg ctttcttgta tgatttcttc ttttaatgtc atggtcttcc tcgcttgctg
    13861 ttaacggctg tataacccat aatatagttt ttcttttttc agctggacaa actggatctg
    13921 ctgatcggca atcttctttt cttccatcca tttttttctt gcagcaaata cttcccaata
    13981 atttgttgaa attaattttc ttttgttatc taatgcatta aaaaaattca atgaaactgg
    14041 ttttgtactt aacagttcat ggagccttac ctcttcccat ggcgaaaact cagacagatc
    14101 gcgggaatac aatacttctt catagagatt tttttgctgt gtgtacgtga atgtttgcgg
    14161 gattttccca ggcctagcaa tttctactag acgattataa aacatattca ttaatttgct
    14221 gttggcaata aattgacttt tataataggc cgactggatc tttttgatta ctccttctgc
    14281 tttgatgact gaaaataatg caggccatgc tttgacggtt atttcgtcgt tttgtttaac
    14341 taatacgcca ggtgtgaagc cgactggaga aaggaacaca aaataaccga taccggtatt
    14401 ttcatctaaa ggctgaaatt catcaaacaa ccatttgcct atgtacagct cttcatctgg
    14461 ttctgttgtc tcattttgat ttgctatgaa taatttagct aattctgctt caagaaattg
    14521 aaggtcttga tccttgtcgg tattttcctg ctggctgatc aatcgaaaga cttcttcttt
    14581 aatcgtatat ttatctttac gtgtaaaatt catagccaca ccttccttcc ttactctaac
    14641 tataacggaa tttcttcaaa aaaggtatct gtacgacgaa aaacagcata attttttttc
    14701 atatctctta taaaaaatgc aaggtctgct cctcaagcta gaccttgcac ttttcagctt
    14761 tcttttgttt gtgacaaacc ttcatggatc gtttcaatat tccatttcat catctgatag
    14821 taagaatctc cttgttctcc ttcttttgct atggaatcag tgaacagttt atcatagatt
    14881 ttcaaacccg tttcttttgc tacccgttcc atgcttcttg agtctacact ggtttccacg
    14941 aataaaaccg gtacttcttt tgctcttatc tgatcaatga tcgctttcat ttgatcaggc
    15001 gtaccttgac tttctgtatt gatctcccat atataagctg ctggcaaatc atatgctcgt
    15061 gaaaaatact tgaatgcccc ttcacttgtt actaataatt ttttgttctc ttcgatagaa
    15121 gcaaaacttt ccttcgcttg tgtatctaat tcttttagtt tttccacata tgcttctgca
    15181 ttttttttat agagtgtcgc attttctgca tctttttcag agaatatacg tgcgatttcc
    15241 tccacatatt ttattccgtt agataggtct agccaagcat ggggatctgc ttttgtatgt
    15301 tcttcaccgc tagttaaata aagaggttct acatttttgc taactgcaaa ataatctttt
    15361 ccttcttctt ttttagccgt ttccatcaag ttatcgaacc agctgttacc tgtttcaaga
    15421 ttcaaaccgt tgtataaaat aacatctgca tcgcttgcct ttttgatgtc ttctggtagt
    15481 acttcatatt catgcggatc tgttccgact ggtacgatac tgtggatatc tatttgatct
    15541 gttccgacct cttttgccat gtccgccaag atcgaattgg tagccacgac tgacaacttt
    15601 tctttacttc cttcatccgc ttggtcagaa gcctgctttc cgcaaccggc taataccaag
    15661 aaactaactg ccaagattaa aaagagtgat ttttttgttt tcatctatct gcttcctttc
    15721 tgaaataaaa ttccttttgt tggagaaaat aaaaaagcaa ggataaaaaa tgcggtacat
    15781 gctaacacga tcgtagctcc tgatggccaa ttgtaactat aactgaaaaa cacaccgaca
    15841 actgcactta gcataccgat tcctgcagct gtaatgatca ttttcagtaa atggttcgtt
    15901 agcaagtaag ccgtggcggc tggtgtgatc aacatggcaa tcaccaagat tgttcctact
    15961 gtctgtaaac tgactacagc cactaatgtc aaaaagaaca tcaaaagata atggatcagc
    16021 gatgtgttca gcccataagc ttttgccatt gtcggatcga atgatgtgat cttcaattct
    16081 ttataaaaga aaaagacaaa tatcaagacg attccactaa cgagtgctgt cagcaacaaa
    16141 tcgctttccc taacagctaa gacattccca aataaaatat gatacaaatc tgtcgcactc
    16201 tgggcatatg aaatcaagat aatccctaat gcaaaaaagg agctaaagac aattccaatc
    16261 gctgtatcat ttttcaatcg gctccgctga gtcacgaaac caatcactgc tgctgttagc
    16321 atcccaaaag cagtggctcc aaagatgtaa ctaaaaccaa acatataaga aatggctaca
    16381 cctggaagaa ctgcgtgaga gatagcgtct cccatcaaag acatccctcg taaaataata
    16441 aaagaaccga tgactccaga agcaagtcct acaagcatgg aagtgaccaa agcgtattgc
    16501 aggaattgat aattcattaa tccatcaata aaggattgga tcattttttc tctcctcctt
    16561 ttactaaatg tctgatgatc tcaccatagg ccaattgaag gttcttcgat gtaaagactt
    16621 cttgtactgg accggaagcg atcagctgtt tatttaaaag aataacctca tcgaaatatt
    16681 cttctacttt atgaagatca tgatggacga tcaaaatcgt ttttcctgaa ttcttcaatt
    16741 cctgcaagat gtcaaagatc tttcgttcac ttaacgcatc aatccctacg aatggttcat
    16801 ctaaaaagat ccattctgct ccttgggcta gagctcttgc aataaaaact ctctgaagct
    16861 gtccacccga tagttcgctg atctgtcttt tggcatattc ttccatccct acttttttca
    16921 aagcttgtct tgcacgttct ttttcttttt tcccaggtct ttgtccaatt cgtaaagatg
    16981 gatatgttcc taaaagtaca acgcctaata catcaattgg aaaggaaaga tccaattcac
    17041 ttcgctgttc cacgtaggct atttttttac ggactgagtt taccgactgt ccagaaaagg
    17101 tgactttacc agtagtagct ggtatcaact ctagcaaaga tttcataaat gttgattttc
    17161 ctgcaccgtt tggtccgata ataccggtaa ttttttgtgg atttatcgtc aagttgatag
    17221 ttttcaaaac agttttccct tgataagcgg ctgaaaggtc ttgaattaca atggccgtac
    17281 gttccaaatc ttacactcct ttttaggttt acctaacttt ttatttaaat atagtttaac
    17341 taaaatggaa agcattttca agtagaatga tgaaaaagtt tttttcgaat tttcctcgca
    17401 ttttatgaaa attttctgta gaatcacatc aagagttcaa taaggctaac ctaataatga
    17461 aaaacatgtg ttgaaacgtt tgattatttt ttcttgttga ctcaacaacg aaaggagttt
    17521 ttgtatggaa gaagaaaagt taactcgaag tcttagtgcc cgtcatatcc aaatgatcgc
    17581 actaggtgga acgatcggtg tcggtctgtt tatgggtgcc tcctcaacca tacgttggac
    17641 agggccctca gtgatgggag cctatgcgat tgcaggattc tttttgtatt tgattatgcg
    17701 tgctttaggc gaaatgcttt acgttgatcc ttcgactggt tcttttgcaa attacgcgag
    17761 cgagtacatc catcctgtgg ctggctactt gaccgcttgg agtaatattt ttcaatatat
    17821 tgtcgtagga ataagtgaag tgattgctgt aggatcttat atgaattatt ggtggcctga
    17881 tttgccggca attattcccg gaatcattgt cgtcttgttt cttatgctgg caaatcttat
    17941 ttctgtcaag gcgtttggcg aattagaatt ttggttttcg atcatcaaag tcataacgat
    18001 cattttgatg atcattgcag gtcttggggt aatcttattt ggatttggta atcatggtca
    18061 ggcagtaggg atttcaaatc tttggaaaaa tggtggattc ttcactggag gcgtcaaagg
    18121 atttttcttt gcattatcga tcgttgttgc ttcctatcaa ggaatcgaac tgatcggaat
    18181 gactgctggt gaagcagaga atcctaaaaa gacgatcatt gaagctgtcc agtccactat
    18241 cggtcgaatc ttgatttttt atattggtgc tatttttatc attgtgagta tctatccgtg
    18301 gaatcaattg agtgagattg gttcaccttt tgtacaaact ttttctaaag tcggcattac
    18361 atttgctgca ggactgatta attttgtcgt gattacagcg gcattatcag gttgcaattc
    18421 agggattttc agcgcaagtc gtatggtcta tacacttgcc ataaatggaa agatgtctaa
    18481 aaaatttctg aaactatctc gtcacggtgc gcctttttat cctgtcgtag caatctcagg
    18541 tggaatctta attggtctga tcttaaatta tcttctacca atgttcatca accagtcaga
    18601 aaatattttc gttttcgtat acagctcaag tattttgcct ggtatggttc cttgggtagt
    18661 gattttgatc agcgagattc gcttcaaaaa gaagcacgca gataaaatgc atgatcatcc
    18721 ttttaaaatg ccactatctc cttatacgaa ttatgtgaca cttgcttttt tagctatcgt
    18781 attgattttc atgtttatca atcctgaaac gagaatctca ttgattattg gactcgtttt
    18841 cttgctttac atgacgatcc attactttgt acgcgaaaaa agaaccggaa aataaaaaaa
    18901 gaacgaagct tcaccttagt ggaagcttcg ttctttttta ttgcttttat caattcaatt
    18961 ttagcgtctc accgggagtc aggaaataat tctccatgct catcccattc agcgacaaga
    19021 tcgtttctac tggtacgcca gttcgttcgg ctatttgatt tggaccttct ccttccaaaa
    19081 ctacggttgt cgcagcctgt gaagtcgcgc tgttggtatt agtttcagag gaatctgttg
    19141 tgtcttgtgt tgttcgagct tgcatttcag aagcttctac cgactctgct gtctcagaat
    19201 cagaagatgt tgtgtcttgt gtgcttgttg actctgcttg aagagaggcg ctagtctgca
    19261 aataaatatc ggaaatctta gcgacatgat tcgtataatt agccgataat tgattgatca
    19321 aagtatctaa gtctacaccg tctttatttt ctgctgcctg atacgctgca cgtaattttc
    19381 tgatcccttc gtcggagatt gcttgaagct cacgtgcttt ttggttagca actgctgata
    19441 aaccagctat tccctcttga tttccctgaa tttctgtctg gtattcttca atcaatgcag
    19501 gcgtcttttc ttcgatcttt ttcgaataat catttacgat tgcttgaagt tgctctggca
    19561 aaccatttgc tgttttttct tcagaagttt gtgtctcttt tgttttagtt gatgactcac
    19621 ttgtttgttt cgtctctgtt ttctttgtcg ttgtttccgt tatggtcgta cttttcgctt
    19681 ctgacgaatt ttttccatga aaatatagac ccgcaactcc tcctgctatg atcaaaaaca
    19741 cggcaactgc tgccccaatc atgatcatcc gcttacgagc tgcctgtttt tgctgtcttc
    19801 ttcgggtcat ccgaacattt tctccccact cattttcatc atacattttt ttcaccacca
    19861 atttttatgt ataatagcgt tatcaaacgc ttcatattct tattgtagca aataattgtc
    19921 gctgtgcaca tttttatctt tgttatttat aagaaaattt caccagttga gatgattcca
    19981 agattgtccg tcgatttctc cttgcagctg cattgttcgt tcgtctatct cagccatcag
    20041 tttctttatt tcatataaga gcgcacggtc ttgatattct gatttttcgc ttaattcatc
    20101 taattgttgg atcagccaat catttgtcat aatgaagttc ctttcatttc ttgcagtgta
    20161 aaggtcaagt cttcgatctg ctcttttgcc tcagtcagtg cttctacgat atatgcttgt
    20221 ttttcagatt cttcttcctg cattttctga ttttcctgta ctctttgaat aaaccagtca
    20281 atccgctgag tcagcgatgg tttgccttcc aatgagagat actgctgata agcaattagc
    20341 ttctcccaac gtttttcctg ggataaatac ataaattgtg ctaaaacgaa tggcggaaga
    20401 taggtcatat ggaatttatt tgcgattcgc tgatacggcc tcaaaaattc ggaaatcgta
    20461 tattcttctt ttcctccagc ttggtattct ttttccgcca cacctgttgt caatacgatt
    20521 cctagttctt tcccttttaa tacatgtgct gctcgttcca agacattatc ttgccaaatt
    20581 tttaaatgag caggagaact ataccagtaa aacggaaact ggaaaatgat ccgatcatgc
    20641 tgggccaaga gctgtagttc agtctctata tctatttttc cgtctggata acttgattcc
    20701 aaatgatgcc aagtagctgg taaaccagca gaagcttctt tgaaaaattg ctgatttaaa
    20761 gattcttcga tcgttggatg gctaatgatg ataagtgtac gcatcccttt tcctcctttt
    20821 tagtcttcgc tttacagtgt agcataaccg ccacaagttt cttttgccga tgcttgtcat
    20881 tttaaccaga ctaatcaggt tgtccaagaa actgcggttt gtttttccaa ctgttttttc
    20941 aaggtatcca aagcaatatt cttaaaattt tcgtttttca tacgtttacg atagttcgta
    21001 gccgtttctc cggtactctt tttgaacatt cggctaaata cgactagact attgaaccct
    21061 acttgatagg caatactttc cacggattca cccgtcagag aaagaagaaa cgcccctcga
    21121 ttgatccgta ccccgatcaa atattccttg atactggtac ccatatgatt tttgaatact
    21181 cgcgacaaat ggctccgatt gaccgctaaa tgctgggcga tttcctcaac agacaatttt
    21241 tcagcataat gtgcctcgat gtatttgact gtttcaacag ctaaagagga atgaaccttt
    21301 tgtacaccac cgctgacctt tccgccatct tttaaaagga ctgataaaaa acggtaggtt
    21361 aatgctgtta actgtaattc attttccaag gtttcttccg tatctttcat gcattctaaa
    21421 ataatgtcga tatatttttg tatgtcagaa gagaccatcg tgtattggtc ttctctgaaa
    21481 agcgactgcc gaatgatttc ttctgcagca ctcccaccaa atgaaagcca gcaatacatc
    21541 cacggatctt ctccatctga taagtaaaac gtcgagtctc ctggacgaat caagaacaga
    21601 tcaccttttt tcaattggaa ttgttgattt ttcacatgat aagatccttt gccttccatc
    21661 acgacatgca ggatgtaatc attgcgaata gcaggaccaa aagaatgaaa cggcatcgtt
    21721 ttagagaaac cacaaaaatc aaaatacaat tctttttcta cttgttgatg ttcctttttt
    21781 aaaaaaatcg attgctccac gtctatccct ccttactcct ttagtataat gcaacaaaaa
    21841 gttaaataaa agtcacagaa atatattaaa aaggaagcga tttcttgtta agtttaagtc
    21901 attaaaagat ctttgttatt cgttgtaacc ggtttactta gtattatata ttgaacagga
    21961 gaggaataca gattatgaaa agcaaaatga tccaacgtgt gtcctacgct ttaggcgctt
    22021 ttggacacga cacattctat gttacattaa gcacttattt tatggtattc gtgacaagcc
    22081 agatgttcgc aggaacagat aaggcaacga atgcaaaaat gattgctaca gttacaagct
    22141 tagttgtagg tatccgctta gttgaaatta tttttgatcc tttgattggt ggcatcatcg
    22201 ataatactcg tacaaaatac ggtaaattca aaccatggtt agttatcggc ggcgttgtta
    22261 gttccgtact cttggtagca atctttacca atttctttgg cttagctact cataatacga
    22321 ctttatttat catattattt gcaatcgttt tcatcttatt ggatagtttt tattcattta
    22381 aggatatcgc tttttggtca atgattccaa ctttgagtac ggaaacaaag gaacgtgaaa
    22441 aatttgctac gtttgctcgt ttcggctctt ctctaggtgc aaatggtact actctagtcg
    22501 ttgtccctat cgttactttc tttacctatc ttgttactca taaacatatt gaaggcgctt
    22561 caggctggtt ctggttcggt gtgattgctg ctgttatttc aggcggtact gcgattatca
    22621 ctgccttggg cacaaaagaa gtcgatactg cgatccgtca gcaaaatgaa aaagctggga
    22681 tcaaagatgt gttcaaagca gttgctcaaa atgatcagtt gatgtggctt gcccttgctt
    22741 atatcgctta tgcgattgcc aatgtagcaa caactgccgt attattctac ttcttcaaat
    22801 atgtacttgg ccaaccaact gaattttgga tcgttggtct agttgctgcc ttacttggtt
    22861 tagtagctgt tccactattc ccaatgctta cacgcgtcat ctctcgtcgc tacgtttacc
    22921 ttggagggat cgcaatgatg atttcttctt actttttctt tacgatcgca ggttcaagtt
    22981 tgccaatcgt tatcatcggg ctagtattgt tctacttccc tcaacaattg attttcttat
    23041 ccgctttgat gacgatcaca gactctgttg aatatggtca atggaaaaat ggtatccgta
    23101 acgaagctgt tactctatca attcgccctt tactagataa aatcgctggt gctttgtcta
    23161 acgggatcgt agggtttgtc gctagcgctt ctgggatgac tggtaatgct actgctgctg
    23221 atatcactgc tcatggcgta acgactttca aagcttatgc attctatgct cctgctgctt
    23281 tgatgatttt ctccgctatt atttttgctt ggaagattac attgacagag aaaaaacatg
    23341 cacaaatcgt tgagaaattg gaaaacaaat tggctccagc tgatacaatg gaagatgaac
    23401 ttcacgatgc agtagttcct tcccattaag aaaggatctt gaaaatgaag attacagaaa
    23461 aagattttgg ccaaggttat cacttgataa ccttggagaa caaaaacaag ctagcacttt
    23521 ctatctctga tttaggtgct agaatcgtca gtttaaaatc aaatgatcga gaattagttt
    23581 tgggatttga caccgctgaa gaatatatcg aaaaagatcc gtatatcggt gcttctatcg
    23641 gtcgcaccgc agggagaatc gaaaatggcc acttttctct caatggaaaa acttaccaat
    23701 tagccacaga tccaaaaact ggacataatc tgcatggtgg tgctcctgga tttgaattaa
    23761 aaaaatggtc ttatgtcatt ttgaatggcg aaaatgaagc ttctgtcatt tttactacta
    23821 caagtcccga cggagaacat ggttttccag gtacaatgga tgtggaaatc cgctatactt
    23881 tgacaaaaga taatatctgg cgagtgacct ctcgcggcac cagtgaccaa gatactttat
    23941 tcaatccgac aaatcatgtt tacttcaact tgacgggaga tgcttctcag tcaatcgatc
    24001 aacatgagct ttggctcaat agcgaagctt atgctccttt gcgaacagat agtattccta
    24061 taggggtaaa agaaaatgct gcaggaagtg ctttcgattt tcaaatacct aaaaaattag
    24121 cgtccgtttt tgctagtgat ttggatcaaa aaaatcttgt tgacggaatc gatcatccat
    24181 ttttcttaaa agaaaccggc ttgggtaaag aagccgctcg cttgactagc cccgataaaa
    24241 gaatccaagt agatattgct acagatgctt ctagtgtggt tattttcact gcaaatttcg
    24301 gcacagaaac accagaaatg cgcaaccgaa aactggcaca tcacggtggc atcacatttg
    24361 agactcaaac agctccaggt gcagagcgct tctcagcatt tggttcgatt catttaaaag
    24421 ccggatcagt atttgaaaca gtgacagaat tcaaaatcaa aacaagaaag gaataactca
    24481 tcatggaaat caaaagaagc ttaaacacaa aatttgccga aacattcgga cctaaaaaaa
    24541 ctacacaata cttctcacca ggacgcatca atttgattgg tgaacacaca gattacaatg
    24601 gaggccatgt cttccccgct tccatcactt atggaacgca aggtgtagca gcacctcgag
    24661 acgataataa aatattagtt tattccacaa actttgacga tatcggtgtc attagtttta
    24721 ctttagatga gctagaatat gataaaaaag caaactgggc aaattacgta aaaggaatgg
    24781 tccttaaatt aaaagaagct ggacactcaa tcgatcatgg gtttgagtta ttagtggaag
    24841 gaacaatccc taatggtgca ggactttcca gtagcgcttc attagaatta ttagtaggtg
    24901 ttgtattaga agatctgttt gatttaaaaa cagatcgctt ggaactagtc caaacaggta
    24961 aaaaagtaga aaatgaattc atcggtgtca attcaggtat catggatcag tttgccatcg
    25021 gcttcggtga gctagatcat gcaatcttat tagatacaaa tacgctgaaa tacgaaatgg
    25081 ttccagtgaa actagatggt tatgcaatcg tcatcatgaa tacgaataag cggcgggaat
    25141 tggcagattc aaaatacaat gaacgccgta gcgaatgcga agaagccttg gcacgtttgc
    25201 aaaaaacttt gcctatctct tctttaggag acttagatga agaagaattt gaatcaacta
    25261 ttgaccaaat cggcgatgat acattgatac gcagagctcg ccatgccgtt tctgaaaatc
    25321 agcggacttt gaaggcaaaa gctgaattag aagctgggaa cttagaagca tttggtcaac
    25381 tgttaaatga ttctcaccac tctcttcgct atgattatga agtaactggg atcgaattag
    25441 atacactagt agatgctgct caaaaacaag aaggtgtctt aggtgcacga atgacaggag
    25501 ctggatttgg tggctgtgcg attgctcttg tcaaagaaga aaatatacct gccttcaaaa
    25561 acaatgtcta tgatgaatac atgaaagttg tcggctatgc tccggaattt tatgttgcac
    25621 acattggaaa tgggacaaca aagatcgatc cagaagctgt tcatgtttga taaaaaaatc
    25681 aacgatctca gctaaaaaca aatagataaa aataacctct cagcaatttc caaatgtgag
    25741 ccttttgctc gtatttgaaa attgctgttt tttattgaca cggaaagaga acgtgtgttc
    25801 taattgtgtt gaagaggtga ttgttatgtg ggcgaatcaa aatccggtat ttgtttatga
    25861 aaaagaacct tcaagagata ttttatgtat tgactgcaaa tctttttacg cctccgtaga
    25921 gtgcgttgaa aaggatcttg atccccttac aactaaatta gtagtcatgt cctatccgtc
    25981 cgattcgaca gaaacacggg gtagcggatt aatcttggct tccagtcctg cagcaaaaaa
    26041 agcttacggc atcaccaata tcagccgtgc cagagatctt cccttcccct atccaagtga
    26101 tttatatatt gttgccccaa ggatggccta ttatatggaa aagaacagag aaatcaattc
    26161 tatctataaa aaatatgcag acgaagcgaa ccatcatgtc tattccgtcg atgaaagctt
    26221 ccttgatgta acaaatagca tggcactctt tggtaaaaca aatgcgtacg acatggctaa
    26281 actgatccat aaagatgtgt ttgagcagac cggaatctac acgactatag gaattggcga
    26341 taacccgcta cttgctaaac tagccttaga caatggctca aaagacggaa agcaaatgtt
    26401 tgctgaatgg cgttatgaag atgtaccaga aaagttatgg aaaatctcag atatcactga
    26461 cttttgggga atcggcaaac gaacagcaaa acgtctgaaa caaatgggta tacattctgt
    26521 ttatgattta gctcacgcgg attattatca cttgaaaagc aagctagggg tcatcggtac
    26581 tcagctttat gcccatgctt ggggcatcga tcgaagtttt ttgggacaag aatatacacc
    26641 aaaatcaaaa agtatcggaa atagtcaaat cctagataga gattatacaa gacgagaaga
    26701 aatcgagatc gttatcaaag aaatggccga ccaagtcggc actcgcttga gacgatctgc
    26761 agcaaaaaca caagtagtca gcttatgggt aggatactct ttaggctatc ttgatccaaa
    26821 tggcaagacc gggtttcacc agcaaatgaa aatcgaagcg acaaatgcta gcagagatct
    26881 agcagatgct ttattgacca ttttccacca gcattaccac taccaggata ttcggcgtgt
    26941 aggcgtgaac tgttccgacc tcgtatacgc gacaggttta cagcttaacc tttttgaaga
    27001 tcctgaaaag cagataaatg aagcgaaagt tgattttgtt gtcgatacga ttagaaaaaa
    27061 atacggcttt caatcaatca tccatgcgca tagtctcttg caaggaggac gcgccatcgc
    27121 acgtagttct ctcgttggcg ggcatgcagg tggtatgagt ggattagaag gtggaaaaga
    27181 tgagacggac agcaaaaaaa ttcattgact ataacgagta tcgagatcgg ccatttggat
    27241 taaaatgggg gactgctttt gcgatggaag aactagtcaa cggtatcaaa aaaaatgaag
    27301 aagaagcacg caaacagcct cttcctaaac aacaaatgac attgcaagaa atcgatagta
    27361 tactgacaga agccttttta tctcgtaaag cagtaatcat tcagctcaat ttaaaagatg
    27421 aatttggaag actcttggat gaggtcgaag ggcacttcat tggtgaagcc tatgaagatt
    27481 actttatgat tgaccatcag ccgatattgt gggaagacgt acggtatatt gagttaaagg
    27541 aaacaaaaaa atggtttgtg acatgagagg ttgtgacaga agtgatcaac ttcaaaaaat
    27601 aagaaaggat ttttcgaaaa ttgcttctta aatttttgtg gaattttggc ttatttttcg
    27661 aagaagcaac ttctggaaca acgtatatta gatatcagag gttgtgaaaa agtcgcttag
    27721 ttttgagtaa caagccaagg cgctgatttc acaacctctg tctatctatc catctattcg
    27781 gtaagattca tgcatgacgt cttctttatt tagtatgtag agcccttttt cttctgacca
    27841 ttcaacataa atcagctgtt ttacatctgc atagccttct ttcttcaagt tctccataag
    27901 ccatgcttca gtcaattgat tggactgaag cactttatgc tgaatttgcc cttcatctac
    27961 aagcaggatc gataaagctt tgtcttcatc tgattttttc aatacactca tttgaccggc
    28021 attctctagg ataacatgtt tggcattttc aagaaaaaag cattcttgct gtcttaagat
    28081 tgctcgcaac tgctccatct cgatatggtt atttgtcaac tctgtcatgt tgatgacgcc
    28141 gtctttgatt agaacagatg gctcgccttt gacccaacga ttgacctttt catttttttg
    28201 gacgatacgt tcgataccat aaatcattac tgcccaaagc gcaatggcaa ataggacatg
    28261 ccctacatgg acattgtcat cataggttga ttcttctaag atcccaccta aaacaagcgt
    28321 atagactaaa tcaaacggtg tgatttctga catggatttc ttcccaagca aacgtaccac
    28381 taataataaa ccgatcaacc cgaacaccag tttcattgta attgaaaaaa tcattttttc
    28441 tccccctaac tatctgttaa ttaaattata tatgctttct cacgaacacg catggaatat
    28501 acaaggaaga aatgtatcct tttactaaaa aaaggactgt gacactgtag tgacccccaa
    28561 aagttagact tttttggagt ggagaaatcc actccttttt tttatgctct aaaatgatat
    28621 ttttacacaa ccgttacggt tgttttacga aattggacga gactttgcca gtttaatttc
    28681 tgttttatcc gttcattatt gtagtaatag atatagctat cgatggctga ttttaattcc
    28741 tcgaaacagt tataaacttt cccgtgaaaa atctcttgtt ttaataagcc aaaaaaattc
    28801 tccataagtg aattatctaa gcagttccct ttccgagaca tgctttgaaa gattttgtac
    28861 tccttcaatt tgtttctata agctttcatt tgatacgccc aaccttgatc tgagtgaaac
    28921 gtacgacgat agggacaatc gttagtggtc tggatagctt cctccaaacc ttccattacc
    28981 gctaatgcat tgggcttttc agagatacga taggataaaa tctcactatt gaacatatcc
    29041 ataaaaggat ctagatacaa tttcttttga cggataatgc ctttggcatc cacttcatgg
    29101 tatttaaatt cagttgtatc cgtcgtgatt ttctgatggc agatactggt gtagaaacga
    29161 cgatggatcc gatttttggc tactgctcca acttttcctc gataagaatt gtagcgacgt
    29221 gatttacgcg tgtatgccgt tacttttatt cctagcttct tgatcaagcg ctgaactttc
    29281 tttttattga tagagaaacc gcggtttctt agttcattgg tcatccgtaa acagccataa
    29341 tccttgtgct tctcccgaat tttaagcatt tccgcttcaa tctcttgatc tggattttta
    29401 cggtccaatc gcctttgcca tccaaataat catgaacgat ctttaattta agttcaaaag
    29461 tatatttaac cataaaaaat accgacctcc aaaagttaga ttttttggtc taacttttgg
    29521 gggtcggtac acaaatcgtg tcacagtccc ttttttgaaa ataaacggtg ttcaaaagct
    29581 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctgttt ttacttggct
    29641 gttcttatta cttgatgcaa ggcagctttt tcacaacctc ttttcaaata tttcgttttc
    29701 ttattctaca aatggttctt ctcctaacgc ttgttgagcg gctagattca acatgctcca
    29761 ttgtcggtca aagcctggct ggaagaagaa gtcagcttct gctagttctt ctaaagtcag
    29821 tttgttttgg atcgctagtg ccaatacatt tccttgacct gtaacatcat aggtagataa
    29881 tactgctcct cctagcaatt gatgggtcat tggatccgag aataattgga tggacacttt
    29941 tggatttcct tgttcttctg gtacatattt tggtcgtaac gtatcttcat aataagacac
    30001 tcgtacatct actcctgctt ttgctgctgt aaagctattc aatccgcttg ctgcaaaatg
    30061 atagtcaaag acacttaatg cagaggaacc tacgactccg ccgaacggtt tgtttggtac
    30121 ttcttcaaaa agatgtttta ctacataacg tgcttctcta cgtgcaacag tcgctaaagc
    30181 gatcggcatt tttttgcgtg ctgggataga ataagcaagt gtagcatctc ctactgcata
    30241 cacatctgga agattcgttt gcaagtattc atttgtttgg atccagccac gttcatctaa
    30301 gtcgacgatc ccttttaacc attctgtatt tgccttcaca ccggcagctt gaatgactag
    30361 atctgctggg atttcttttt cacttgtttt gattgcctca acttttccgt taccagtaaa
    30421 agcttcgatt tttgcatttg tcaaaacttc aataccgttt tcatttaggt gttgttctaa
    30481 aatatctgtc atctcagcat ctaaataagt gcctaaaggt ctatcgatca catcaagcaa
    30541 tacgacttct ttcccagctt ttcggcttgc ttctgctgct tcgatcccaa tgtaacccgc
    30601 acctacgaca gcgatttttt taatagccgg atcatttaat ttttctttga ttttggttgc
    30661 ccaattgtat ccacgcatca agtagacatt ttctagatct gctcctggta ctggcagatt
    30721 gtttggtttg acaccagaac tcaaaatcaa tttgtcataa ggatattctt ttgtttcgtt
    30781 atctgatgcg ctagtaaccg ttactgtttt tttatcggca tcgatcgttt ttacttcgtg
    30841 attatttaaa atatgaacat tggattgcgg aaagttttct ggtctgaaat tacgtacatc
    30901 atttacatct gttaccttgt cttctaaata cagctccata ccacatgaca tgaaggaaat
    30961 atagtctcct gcttcaaata gcgtgatatc tacattttca ccgtatcgtg tcaatagttc
    31021 caagatcgat tgatgccctc catgagatgc acctacaatg acaactttga ttttttccat
    31081 tgatgaaacc tccttgaata ttcttttaaa atcattctaa acaatttaaa cttaacatta
    31141 cattagttat gaatcaaaag aaacgcgttc atttgaacta aatttaaaaa acaatcgtct
    31201 ccttgcagaa tataccgcaa aattaacgat tgtttttttg ataaaatcta tgaatcactg
    31261 ttctgttaaa aagtctttga tccgtttgca ggcctcatgt aatttttcca tgctgctagc
    31321 ataagaaatc cggatataac cttctccttc tgggccaaat gcaattcctg ggatacacgc
    31381 aacttttgcc tgttccgcta attctacaca aaactgccaa gagtcagttt gaatggcagc
    31441 tgggagtttg caaaacaagt aaaatgcacc gtttggctgg gtcacttcaa atcctaaagg
    31501 cgccaattgc tggaccagat aatctcttcg tgtttgatat tgctctttca tattctcgct
    31561 atctttcacc ccatttatta gcgcttcagc cgcagctttt tgcgacatcg ttgtagcggc
    31621 agtcaccaga tattgatgga ctttaattag ttcgtcaata agggcttttt gagcaaaaat
    31681 aaagcccact cgccaaccag tcatcgcatg tgacttggat aacccgctta tcacgattgt
    31741 ttgttctgga agataagtag ctatggacat atgcgttcca ctataagtca attcagcata
    31801 tacttcatca cttataacaa ataccggata tttttttaaa acctcagcga ttgctttcaa
    31861 ttgttttgca gacaatgtcg tcccagtagg attgttagga tagtttaaaa tgactgcttt
    31921 gacagctgat ccatattgtt caaatgctcg ttcaaattgt tccggctgac agacaaaccc
    31981 tgtgtcagaa gtatctatcg ggatcaattc agcaccagct agttccacta atggctgata
    32041 tcctgaataa gcaggcgctg gaataagaac cttgtctcct tcttccaaaa cagataacaa
    32101 cgcagaggca atcgcttccg ttgcaccaac tgtcgtcaaa acttccgttt gcggatcata
    32161 agttaatcca taacgctgtt gctgaaatat acaagccgct tcccgcagtt cctctaatcc
    32221 tctcattccg gtatagtgag aaaagtcttc ttcaatcgct gctattcctg cttgcttgac
    32281 atgttcagga gttggaaaat ccggttctcc cagggtgagt ttgatcacat ctggtatcga
    32341 cgatatttgc tgatcgaatt gtcgtatttt tgatacttct attctagaaa gcctaggact
    32401 aaatcgattg gataaattca tcattattta ctcgctcctc ttttgaactg ctatcgattt
    32461 ttacaagaag attttctgta aaaagaatcg ttttgtacca ttctacacca ttttctaaaa
    32521 catccatatc aaaatcgaac gtcggatcat gtaaagcagg ggtatcaccg acacctaaaa
    32581 agaaaaatac acatggatat tgttgcgtat atatgccaaa atcttcggct tgaagaactg
    32641 gagcatctaa ttcatgtagt ttgtttgaat ctaacagtgc tgcatataag cctctatggt
    32701 tgattacagc tggatagccg tcattatagc gaaaagaaat agtagctgta gagtgaaact
    32761 gttctgctaa atggtctaat cccctcttca aataattttg catatctgaa ctgtaagaac
    32821 ggatacttcc ggatacaata acttctccag ctaaaacatt tcggatcgtt ccaccttgga
    32881 tttggccaaa tttcattaga tgattccact ctgccggtaa ttgccggtca aaatcataaa
    32941 cattcttcaa aaatcgtaca gctgcctcca gactatcgat tcctttttca ctagaagcaa
    33001 tatgtgcaga ctggccttta atgataatgt ctgtttcact gctttgtgcc atcaaaggtc
    33061 ctggtcgaga gaaaacttct cccttagcta gtccaggcca tagatgaaag ccaaatattg
    33121 cttgtacttc atatttatcc aacaatcctg actgaataac gctttgtgcg ccaccggaaa
    33181 tttcttctga tggctgatag atcagtacta cattataaat aagatcctca atgtgtttag
    33241 ttataaagtc agtcaaccct aaaagcatcg ccatatgtcc gtcatgacca catgcatgca
    33301 tataaccttc atgcttagaa gcaaaagaga gattcgtttc ttctttgact ggcagtgcat
    33361 cgatatctgt tcgaaaagca atcgtttctt cttgctgatt atcaaaataa aggagtagtc
    33421 ctgtttcgcc gatttcatgg ataacggcct cgctgttttt tacttgttca tataaatatt
    33481 tttttgtctt gaattcatga aacccgattt caggaatttg atggagtgcc attcgatatt
    33541 ctttcagcat ccgtattcac catcccttca tagataccct tcactactgt ttcatattga
    33601 ttattcggta caccgatgat cagattcaac tcctgtgctc cttgtgataa gatcgatgtg
    33661 cgaatttcta atttgttcaa tatcgataac actttcccag ataatccaat caattcttta
    33721 tgcggacccc caacgacaga gatcaaagct aagtcttcga tcacgacgat ttcttccacg
    33781 ccaagtgttt cttttaattc tttcgtgatg agaaataact tatcggcaat cgcttctgct
    33841 gagacgatca ctccgatatt gtcgatccct gaaggaatat gttcgattga tatctcatgt
    33901 ttagcaaaaa tgctcattgc tttccagata aacccgattt catcggacat atgacgtttg
    33961 aataaagtga tggaaagaaa atccttgcgt ccagctattc ctgtcagtcc attttccttt
    34021 tctatttcgt gattcgatat ttttgtccct tgagcgaaag gatcattggt attttttatt
    34081 tggatgggga tatcagcctc ttgaactggg aaaacagctg cttcatgaag aacgttagcc
    34141 cccatataag ccatctctct cagttctcta aaagagattt cctcgatgat tttaggatga
    34201 tcgatgattc gcggatcagc catcatgatt cctgaaacat ccgtccaatt ctcataactt
    34261 tccgcttgca acatcttcgc cagtatcgcg ccggtaatgt cgcttccccc acggcccaat
    34321 aatttttctt ttcctgcagg gttcgccccg taaaaccctg gaacaaccat tttcggatat
    34381 cttttaaatg cttttgtgat ttcctgtgct gttttttcct gatcgatttt cccatcatag
    34441 tcaaagaaaa tcaggtcttt tgcgtcaata aactggtaac caagatactc agccatcagt
    34501 tgagcagtca gatattcacc tctacttact aaataatctt cagttatctt ttcgtcttcc
    34561 agttgtttct gtatgcgtgc tagttcatct tcaataggta actgcagggc aagaacatct
    34621 cttacttgaa tgaaacgatc acaaatcctt ttccaaaggg gtgtccaatc aacatgatgc
    34681 tgaaggtaag cataaatcaa ataaagtaag tctgttactt tgtcatcatc tgacgtacgt
    34741 ttgcctaaag cagaaacaac gatcacttgg cgattgggat ctgaaagaac aattgtacgg
    34801 acttttttga attgctcttc atgagacatc gatgttccgc cgaatttcgc tactttcagc
    34861 gtattactca tcttgccacc tccgctattt tctgcattaa agctgcagca atctgtacgg
    34921 cattcgcagc ggctcctttg cgtatgttat cggcagtcgt atagaacaac aatccgttct
    34981 ctaaagaagt gtcttttctg atcctcccaa cataaaccat atctgttcct ctagcaagaa
    35041 tgctcgtcgg atatttatgt gctgctagat catcttctaa ttgaatccct ggaaactggc
    35101 tgagacactc tctaatttct tccgtcgtaa actctttttg cagttgaact gccacagaga
    35161 ctccatgtcc attttcgatt ggcacacgta cgcaggttgc tgatactggc aaagcaggct
    35221 gatgcagaat cttacgggtc tcatttatca ttttctgttc ttcttttgta tacccatttt
    35281 ccaaaggctg atcgatttcc ggaatcaccg tttggctaat attataagga tacaaaactg
    35341 gttcctcgtc attttgcgta cgatgaagat cttcaatgcc tttttgcccg gaaccagaaa
    35401 ctgcttgata ggtcgaataa ttgactcgct ggataccaaa ggcttcttgt aaagctttaa
    35461 gcggaagtac cgcttgaatg gttgaacaat ttggatttgc aatcagccga ttcaatgtat
    35521 agtcttccag attgacctct ggaacgatta atggaatatc cgaatcttcc cgccatgctg
    35581 aggaattatc tatgacaaat agtccctgac ttcgtgcgat tggcgcaaac tctttggata
    35641 cagaagcacc tgcagaaaat agcgctaaat catatccttc aaatgaagtt tctgtcaatt
    35701 cctgtacagt gtgtttcttt ttcttgaata cacaaacttt accggctgaa cgttttgaag
    35761 ctaataattt caatcgtcct actggaaaat catattcttc taacactttg agcatcgttt
    35821 ggccgactaa tccggttgcc cccacaatac aggtatctat ttcgcgcatt tttctccatc
    35881 tcctctataa ttcgcgtaac gcagcaatca atgctgtatt ttcttttgtc tgatcatcac
    35941 ttatttttaa tacacgtgca ggtattccac cgacaacagt ttctgctgcc acatcttcta
    36001 aaaccactgc tcctgctgcc acgactgcat cttttccgat atggacacct tcaacaatca
    36061 ctgcattcgc acctaccagt acgccgtctt ctacgataac cggtttggcc gatgctggtt
    36121 cgatcacacc tgctaagaca gctcctgcac caatatggca gtttttgccc actgtcgctc
    36181 gtccacctaa gacagcaccc atatcaatca tcgtgttttc accgattact gcaccaatat
    36241 tgataatcgc tcccatcatg atcacggcat tgttgccgat tgacacttga tctctgataa
    36301 tagcacctgg ttcgatacga gcattaattt ttttaatatc tagcaaagga actgcgctgt
    36361 atcttgcagc agtttcgatt tcgatttcaa tgattttttc tgattcagct tctaaaaccg
    36421 gagcaatcgt ctcatagtca ccaaaaacaa cgaagctgct tccttcacca aaaactttac
    36481 tttctttaaa ttcgattggc tcggttacat tcagatagac tttcacaggc gtctttttag
    36541 atgccgtttg aatgtagcgg atgatttctt gagcttccac gttttttaca cccctttgtc
    36601 cattaataaa tcttctaatt tgtaataacc tggtgactga tctttcaacc agtccgctgc
    36661 ttctactgcg cctgcagcaa agattcgatt agaaaaagat tcatgtttga tcgtaaatat
    36721 ttcatcagta gcagcaaaca atacatcatg ttcaccaggt aaagaacctg cacgaatact
    36781 gtgtacacca atttggtttt cttctctttt tttcccttgc cagttgtata cgggctgcag
    36841 cttttgcact tcttggattg tttgaagcag catatttgca gtaccagatg gtgcatcttt
    36901 tttacgacta tgatgttttt caatcagttc gatctgccag ccttgcaaca ttgcagctgc
    36961 ttgcttgatt agttgattca tcaccatgat gccgaacgaa aaattagcac tatacatcac
    37021 aggtacatgt ttggcatgtt cttttatttc ttggaactgt gattgtgtat aacctgtcgt
    37081 gccaatgact aacggaagtt gatatgataa gctatacgaa aggattttct ctaaattatc
    37141 tggatgagaa aaatcaataa gcagttctgg cagttccgta aaagcaagcg tagttgcttc
    37201 tttaggtgcc gtccgtaaaa gaaggaccgt atctcctctt tcttgtgcaa cttcttcgac
    37261 tctatttccc atctgtccac tgccaatcag tccgattttc atttttcaac cgtcctttct
    37321 tttaacaaaa gctgagctaa ttgtttcttt ttctcttttg aaagagaaac taaaggcagg
    37381 cgtagaggtc cggaaggtaa gtctaataat gacatggctg ctttgacagg aataggattg
    37441 gtctcactga acaaggcatg gatcagttca agtgcattta attgcttttc ttttgcttgc
    37501 tgccatcttc cgtcctgcgt atcttttact aattcatgga ctattttagg ttggatattc
    37561 gcccatacac ttattacacc gcttcctcct acactcatta ctggtaggat caaatcatca
    37621 tttccactgt aaaggaagaa ttcttctcca atcaaccgag cggcatccat cacataagcc
    37681 atatctcctg aggcttcttt caaaccaatg atattaggat gttctgctaa atttactaat
    37741 acatggattg gaatcgacat gcctgttcta gaaggaacat tatacaaaat aagcggaatc
    37801 ggtgaggctt ctgcaattgc tgtaaaatga gcagctagtc ctgcatcact tgttttgttg
    37861 tagtaagggg tgatgaccaa aagtgcgtct gcccccaaag aagcaaaatg cttcgctttt
    37921 tcaatcgttt ctttcgtatt attcgttcca gctcctgcaa tcaccggcac acggccagcc
    37981 actttcttta ctgtcagttg aaggatctgc tcctcctcag attgtgtgag cgtagaagat
    38041 tctcctgttg tacctaaaac taataatcca tctgtttgtt catctaaatg gaattggatc
    38101 aatttttcca aaccagcata atctactgat ccctcttcat tcatcggcgt gatcagcgcc
    38161 acgatcgaac cttttatcat cttcattcct ccaatattct tgtccattca tgaactgctg
    38221 ggccagccat ttgtatcgca tcctcaccag agatcatcaa gtctccttgt tccaagtgaa
    38281 caactacctg atctgctgaa attttcccag tttccttagc aaccacataa gaggcacagc
    38341 aacctgtacc gcaagcaagt gtccaaccta ctcctctttc ataggtacgg acgatcaatt
    38401 cttgttcatt gactacttct acaaagttga cattggtttt ttctttgaac aaaggatgat
    38461 gacagatcag ttcacctctt tttctccaga cttcctcacg cgcattttca acaaaaacaa
    38521 cggtatgaat cgttcctaaa aataaactag tgatcgttac tttttcacca ttgatctcta
    38581 tttcctggtt ttttattgga taagtttgat tagctttgat tttttccgga gaatcatctg
    38641 gcactcccat atctacgctg caataaaaag gtgagggatc caacaccgta atttttcttg
    38701 tgcctgctaa tgtctctacg ttgaattcct gttcattcat catgttctga tcatttacgt
    38761 atcgagcaaa acagcgaatg ccatttccgc acatcggcgc gcggctgcca tctttgttgt
    38821 aatagatcat ttccaacgga tcgattttta cggcgatcaa tccgtctata tcatattttt
    38881 cttttgtgca taaagaagcg gctaattgac tataatctgt gtcttctgcg taatctaata
    38941 agataaaatc atttccgcag ccattatatt tatgaaggat cataggaaac ttcccccctt
    39001 agtagatcag aaaatgcttg tctgcgaaca atcagtcttg attggccatt attgacaaaa
    39061 acaactgctg gtcgtgtcgc tcgattgtag ttgctgctca tcgagtatcc ataagctccc
    39121 gtggaataaa cagctaaaag atcacccgat tctgctttcg gcaaataagc ttctttaatt
    39181 accacatcgc ctgattcaca catttttcct gcaatcgtca ctttctctgt tttttcttcc
    39241 gacagttttg ctgctaaatc acaatcgtat tctgcctgat acagagctgg acggatatta
    39301 tctgtcatcc cgccatctac aaagtaatat tttttatgtg gtgtcttttt gatgaaacca
    39361 atcgtgtata gtgttgtacc agcttctcca acaatacttc tacccggttc gatcagtaat
    39421 tgacgaatgg tcagttttcg tttttccaaa gcttcttccg tatattcaat cagttttttt
    39481 agtgcctctt cgactgggat cggctgatca ttttgtgtat aacgaatccc aaaccctccg
    39541 ccaagattca aggaaagagg ttggtcaaaa tcggttaagt aattcgtcaa tttgtcgatt
    39601 tcagctaacc aagcagtaac atcaaaaatt tgtgacccga tatgagcatg aaatccttct
    39661 aactctagat agctgctttc ttttatcaac tgaagggctt gttggcactc tttactttca
    39721 tagctcatgc cgaattttga atcaatatgc gcagtgacga tatattcatg agtatgtgct
    39781 tctactccga cattcaaccg aagcatgata cagatttttt ttaaatagcg ttggcttaat
    39841 tctgctaata attcaacttc catcaaatta tcggcaacaa tatgcaacaa atcattttca
    39901 agtgcataga tcagttcctc tggcgtcttg ttgttaccat ggaagtagat agtatctcta
    39961 ggaaaatcag cttgcaaagc ggtatagatt tccccaccac tgacaacatc tagcccgaat
    40021 ccatattcac gaactaaatg cagcatctca atcgtttgga atgctttaga ggcgtaaagc
    40081 acttttgtat caaaacgttt cgatacaaag cctcgcttga atgacagcat cgtttgtctc
    40141 atcttttctt catcatacac atacagaggt gtcttatact tttctgccaa tgtgcttgct
    40201 gtgactccgc caattatcaa ttctgtcatt ttttcattcc tcttttcact ttttttccgt
    40261 ttttattgga atttcatttg ataaagattt gaattttatt ggaataatat taaacaaaat
    40321 tttctgaatt gtctagtata tttcgaaata tttttttaaa aataagaata aaaatggtta
    40381 taaaaacaaa atacccgaac tattagaagc tatttataca taaaaacata aattcaacac
    40441 tatttagtga tgaaaaaata ttttcgcttc aatatagttc ggaatagttc ggatattttc
    40501 aattttatga atcatttcaa tttcttcata aataagtgca gttttttctt tctctagctt
    40561 ttgccattca tctttcaaaa tataaataaa atcaaatact tttttatttt tcaatttgct
    40621 gattccacaa aaaacctctt gaaaggcaga tccgatgatc tgcctttcaa gaggtcaagc
    40681 tctgttttca aattcttatc aatccgttag cgaattattt tgacgcacgt tttacataag
    40741 aaccttctgc tgtattgatt tccaacaatt catctgtttc gatgaagtca ggaacattga
    40801 ctactaagcc agtttccatt gtcgctggct tacctgaacc agtgactgtt gcacctttga
    40861 tggaaggttg tgtttccaca acacgtaata cgacagttgt tggtaattga acgccgatga
    40921 cttcgctacc gaaaaattgg atttttactt ccatgttttc taggatatat ttcatttctt
    40981 cttctactgt ttctacagga atttcatatt gctcatatgt ttccaaatcc atgaagaaag
    41041 ctgtatcatc catcgtgtac aaatattgta caggttttgt gtcgatatgt gctttttcaa
    41101 atttttcttc tggtctgaac gttgtgtcgt atgttgcacc tgaacggaca tctttcagtt
    41161 tcatacgcat aactgtattt cctttaccag gtttatggtg gcttgcatcc agaactttaa
    41221 tcagtttgcc gtcttgtacg aaagtcatac cggctcttaa atcacttgct gagatcatta
    41281 tttttgctcc ttcttttctt tatcataatc aactttattg tatcagacaa ccggccagaa
    41341 aactagagta ttctctatct tttaaggtct catgcctatt tgtcagaaaa ataaacacgg
    41401 gtaaacgatg ctatcgaaat atttttttct ttcaagcctt tgacttttat tttttttcat
    41461 gctatcgtta ataaaaattt tacttggagg gatacacatg acagtcagca accaaaaaca
    41521 attgaatcgt tgctgttgta tggtgcataa aagcagcata ctatttgtca tgtctattaa
    41581 ttgcccttca tagaatgagt accaggtaac ttatcttttt atctactcaa tttttctcta
    41641 tgtctttcaa ggagctgatt taatagtcag ctcctttttt tattatcttt ttttaggagg
    41701 cccacatgat tatctcgaca attacagaag ctattggttc cacaccgctt tatcgctttg
    41761 acaatcagca ctattctatg cctaaaggtt ctgctattta tgcaaaacta gaatatttaa
    41821 atcctggagg aagtatcaaa gatagattgg gcagttattt gatcaaagaa ggatttgccc
    41881 aaggaaaaat caatgaaaaa acgacgatca tcgaaccaac tgccggaaat acaggaattg
    41941 ggatcgctct ggccgcactc acatacaaat taagaacggt ttttgttgtt ccagagaagt
    42001 ttagcttaga aaaacaacaa ttgatgaaag cattgggagc aaagatcatc cattctccta
    42061 gtgagcaagg aatcatcggt gccatcagca aaagtaaaaa acttgcagag gagatatcaa
    42121 acagctatct gcctcttcaa tttgaaaata aagataatcc tgctgcttac tatcatacgt
    42181 tagggccaga aatctttcat gaaatcaaag aaaatatcca tagttttgta gcaggaattg
    42241 ggagtggtgg aacctttgct ggaacttcta cctttttgaa agaaaaatat cccgacattc
    42301 gaataatcgg tgtagaacca gagggctctg tgttaaatgg tggtgatcca gcccctcacg
    42361 aaattgaggg tatcggtgta gaattcattc cgccgtttct ttctcccctt tcaatcaatc
    42421 agatcgagac aatctctgat gtagaagggt ttaactatac taggcagtta gcacgagaac
    42481 aaggactatt agttggaagt tcgagtggtg cggcttttgc agcagcatta agagaaattc
    42541 gtcgtttacc acctggtcat cgtgtcgtta cgatttttcc agacgcagca gaccgctatt
    42601 tatcaaaaaa catttactta taggaggaaa gaaacatgca cattcaaaca aaattgatcc
    42661 acggaggcat cagtgaagat cccacaacag gagctgtcag tgtacctatt tatcaaacat
    42721 ccacttatcg gcaagatggc gtcggccagc ctaaacaata tgaatattca agatctggta
    42781 atcctactcg ttttgcattg gaagaactga ttgctgattt ggaaggcggt gttcgaggat
    42841 tcgctttttc ttctggtctt tctgggattc atgccgtttt ttctcttttt caagctggcg
    42901 atcatatctt attaggagat gatgtctatg gaggaacatt ccgtctattt gacaaagttc
    42961 ttactaaaaa tggtctcgag tacacgatca tagatacgag taatcttgat aaaatagaac
    43021 aatccatcaa acccaataca aaagcacttt atcttgaaac acctagcaat ccgcttctaa
    43081 agattactga tttagaaaaa agtgccactc tggctcatca acatggattg atagttattg
    43141 ctgacaatac atttgctact ccttattttc aaagaccttt agatctcggt tctgatatcg
    43201 ttgtccatag cggtaccaaa tatctaggcg gacacagcga tgtagtagct ggtttagtga
    43261 cttctaacca taaagatcta gccgatcaga tcggatttta ccaaaatgct atcggagccg
    43321 tacttggtcc ccaagatagc tggttacttc agcgcggaat caagacacta agtgttcgta
    43381 tggaggaaca tcaaaaaaat gcttttgtgg tagctgattt tctattttct catccagcag
    43441 tagaaaaggt ctactatcca ggattacccg atcatgaact ccatggagtt gctaagcagc
    43501 agatgagcgg ctttagcgga atgatttctt ttactttaaa aaatgaagaa tctgctattc
    43561 catttgttga atcactccaa ctatttactt tagcagaaag tttaggtggc gtagaaagtt
    43621 tagtggaaat tccttctgtc atgactcatg cgtctattcc aaaagaaaaa cgagatgagg
    43681 ctggcataaa ggacggcttg atccgtcttt ctgtaggaat tgagtatggt caggatctca
    43741 taaacgattt agctcaggca tttgacagaa tcaagaacta gaagtcccaa taaacgactt
    43801 agcagaaaaa tctaaaaaat atttcagatt gctacacctt ggaaacgata agtgttagaa
    43861 tcaagtagaa agacgttttt gtaaatttca ctaaataatt ctttagtgaa aagcatcaaa
    43921 acgaatgaac aaacagatag gagtcttaca aatgagaatg aatgtcgaaa gttttaatct
    43981 tgaccataca aaagtcaaag caccttatgt acgtgtagct gatcgaaaaa cgggtgcgca
    44041 tggcgatgtc atcattaaat acgacgtccg ctttaaacaa ccaaataaag aacacatgga
    44101 catgccaagt ctacattcac ttgagcactt gactgccgaa ctaatccgca atcacgcaga
    44161 ttacatcgta gactggtctc caatgggatg ccaaacaggc ttttacttga ctgtattgaa
    44221 tcatgaaagc tacgaagaca ttttagatgt tcttgaagca acaatgaaag atgttcttga
    44281 agctactgaa gtacctgcaa gcaatgaaac acaatgtggc tgggctgcca gccatacact
    44341 agaaggtgca caagctttgg ctcgtgaatt tttagcaaaa cgcgatgaat ggtctgaagt
    44401 agaagcatag tttctaaaag aacgtaaaga tgagaagcaa tatagctatc ttatcttcgt
    44461 attttattaa atatttattg tctgaaaaga gttctaaacg ggaaaacaaa tagctagttt
    44521 tcagtttagc gctcttttta ttatataaaa tagaaaggag aaaactttat gtctatttat
    44581 aattttgaaa ctactttaga agacggcact gtctattcat tgaaaaaata tgcaggcaaa
    44641 ccgataatca ttgttaacac tgcaacaaaa tgcggtctag cacctcaatt taaacaatta
    44701 gaagaacttt accaagctta tcagacacaa ggtctggtta tcttaggctt tccttccaat
    44761 caatttaaac aagagctaga ttcagcgact gaagcagcag aagcttgtaa aaccacttat
    44821 ggcgttacct tccctatgca tcaattgatt gacgtaaatg gagaaaatac agatcctctt
    44881 tttacttatc ttaaaaatga agctcctggt actgtcacta aagcaataaa atggaatttt
    44941 actaaatttt tgattgatag aaatggcaaa gtaatcgaac gatttgcacc acaaacttct
    45001 ccattgaaaa tgaccgctgc aattgaatcc gttctttaac ttaataaaaa
//
LOCUS       DACDIH010000018        44070 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDIH010000018 DACDIH010000000
VERSION     DACDIH010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.018"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            312..620
                     /gene="rpsJ"
                     /locus_tag="IV281_001005"
     CDS             312..620
                     /gene="rpsJ"
                     /locus_tag="IV281_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP8780036.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            651..1280
                     /gene="rplC"
                     /locus_tag="IV281_001006"
     CDS             651..1280
                     /gene="rplC"
                     /locus_tag="IV281_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP8780037.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            1308..1931
                     /gene="rplD"
                     /locus_tag="IV281_001007"
     CDS             1308..1931
                     /gene="rplD"
                     /locus_tag="IV281_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP8780038.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            1931..2221
                     /gene="rplW"
                     /locus_tag="IV281_001008"
     CDS             1931..2221
                     /gene="rplW"
                     /locus_tag="IV281_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP8780039.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            2260..3093
                     /gene="rplB"
                     /locus_tag="IV281_001009"
     CDS             2260..3093
                     /gene="rplB"
                     /locus_tag="IV281_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP8780040.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            3136..3414
                     /gene="rpsS"
                     /locus_tag="IV281_001010"
     CDS             3136..3414
                     /gene="rpsS"
                     /locus_tag="IV281_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP8780041.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            3437..3784
                     /gene="rplV"
                     /locus_tag="IV281_001011"
     CDS             3437..3784
                     /gene="rplV"
                     /locus_tag="IV281_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP8780042.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            3798..4454
                     /gene="rpsC"
                     /locus_tag="IV281_001012"
     CDS             3798..4454
                     /gene="rpsC"
                     /locus_tag="IV281_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP8780043.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            4457..4891
                     /gene="rplP"
                     /locus_tag="IV281_001013"
     CDS             4457..4891
                     /gene="rplP"
                     /locus_tag="IV281_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP8780044.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            4881..5069
                     /gene="rpmC"
                     /locus_tag="IV281_001014"
     CDS             4881..5069
                     /gene="rpmC"
                     /locus_tag="IV281_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP8780045.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            5094..5360
                     /gene="rpsQ"
                     /locus_tag="IV281_001015"
     CDS             5094..5360
                     /gene="rpsQ"
                     /locus_tag="IV281_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP8780046.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            5418..5786
                     /gene="rplN"
                     /locus_tag="IV281_001016"
     CDS             5418..5786
                     /gene="rplN"
                     /locus_tag="IV281_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP8780047.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            5823..6131
                     /gene="rplX"
                     /locus_tag="IV281_001017"
     CDS             5823..6131
                     /gene="rplX"
                     /locus_tag="IV281_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP8780048.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            6159..6698
                     /gene="rplE"
                     /locus_tag="IV281_001018"
     CDS             6159..6698
                     /gene="rplE"
                     /locus_tag="IV281_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP8780049.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            6717..6902
                     /locus_tag="IV281_001019"
     CDS             6717..6902
                     /locus_tag="IV281_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP8780050.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            6938..7336
                     /gene="rpsH"
                     /locus_tag="IV281_001020"
     CDS             6938..7336
                     /gene="rpsH"
                     /locus_tag="IV281_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP8780051.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            7368..7904
                     /gene="rplF"
                     /locus_tag="IV281_001021"
     CDS             7368..7904
                     /gene="rplF"
                     /locus_tag="IV281_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP8780052.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            8065..8421
                     /gene="rplR"
                     /locus_tag="IV281_001022"
     CDS             8065..8421
                     /gene="rplR"
                     /locus_tag="IV281_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP8780053.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            8442..8942
                     /gene="rpsE"
                     /locus_tag="IV281_001023"
     CDS             8442..8942
                     /gene="rpsE"
                     /locus_tag="IV281_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP8780054.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            8957..9136
                     /gene="rpmD"
                     /locus_tag="IV281_001024"
     CDS             8957..9136
                     /gene="rpmD"
                     /locus_tag="IV281_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP8780055.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            9180..9620
                     /gene="rplO"
                     /locus_tag="IV281_001025"
     CDS             9180..9620
                     /gene="rplO"
                     /locus_tag="IV281_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP8780056.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            9620..10915
                     /gene="secY"
                     /locus_tag="IV281_001026"
     CDS             9620..10915
                     /gene="secY"
                     /locus_tag="IV281_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP8780057.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            10977..11624
                     /locus_tag="IV281_001027"
     CDS             10977..11624
                     /locus_tag="IV281_001027"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP8780058.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            11815..12033
                     /gene="infA"
                     /locus_tag="IV281_001028"
     CDS             11815..12033
                     /gene="infA"
                     /locus_tag="IV281_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP8780059.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            12066..12182
                     /gene="rpmJ"
                     /locus_tag="IV281_001029"
     CDS             12066..12182
                     /gene="rpmJ"
                     /locus_tag="IV281_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP8780060.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            12200..12565
                     /gene="rpsM"
                     /locus_tag="IV281_001030"
     CDS             12200..12565
                     /gene="rpsM"
                     /locus_tag="IV281_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP8780061.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            12593..12982
                     /gene="rpsK"
                     /locus_tag="IV281_001031"
     CDS             12593..12982
                     /gene="rpsK"
                     /locus_tag="IV281_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP8780062.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            13062..14000
                     /locus_tag="IV281_001032"
     CDS             13062..14000
                     /locus_tag="IV281_001032"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP8780063.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            14028..14408
                     /gene="rplQ"
                     /locus_tag="IV281_001033"
     CDS             14028..14408
                     /gene="rplQ"
                     /locus_tag="IV281_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP8780064.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            14680..15519
                     /locus_tag="IV281_001034"
     CDS             14680..15519
                     /locus_tag="IV281_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8780065.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            15495..16364
                     /locus_tag="IV281_001035"
     CDS             15495..16364
                     /locus_tag="IV281_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8780066.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            16354..17151
                     /locus_tag="IV281_001036"
     CDS             16354..17151
                     /locus_tag="IV281_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8780067.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            17354..18100
                     /gene="truA"
                     /locus_tag="IV281_001037"
     CDS             17354..18100
                     /gene="truA"
                     /locus_tag="IV281_001037"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8780068.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(18555..21224)
                     /locus_tag="IV281_001038"
     CDS             complement(18555..21224)
                     /locus_tag="IV281_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8780069.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(21462..21650)
                     /locus_tag="IV281_001039"
     CDS             complement(21462..21650)
                     /locus_tag="IV281_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP8780070.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            21920..22282
                     /locus_tag="IV281_001040"
     CDS             21920..22282
                     /locus_tag="IV281_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8780071.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            22334..24016
                     /locus_tag="IV281_001041"
     CDS             22334..24016
                     /locus_tag="IV281_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP8780072.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            24133..26169
                     /gene="recG"
                     /locus_tag="IV281_001042"
     CDS             24133..26169
                     /gene="recG"
                     /locus_tag="IV281_001042"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP8780073.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            26218..27219
                     /gene="plsX"
                     /locus_tag="IV281_001043"
     CDS             26218..27219
                     /gene="plsX"
                     /locus_tag="IV281_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP8780074.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            27341..27583
                     /gene="acpP"
                     /locus_tag="IV281_001044"
     CDS             27341..27583
                     /gene="acpP"
                     /locus_tag="IV281_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8780075.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            27851..28855
                     /locus_tag="IV281_001045"
     CDS             27851..28855
                     /locus_tag="IV281_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8780076.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            28856..29800
                     /locus_tag="IV281_001046"
     CDS             28856..29800
                     /locus_tag="IV281_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8780077.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            29800..30762
                     /locus_tag="IV281_001047"
     CDS             29800..30762
                     /locus_tag="IV281_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8780078.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            30789..31703
                     /locus_tag="IV281_001048"
     CDS             30789..31703
                     /locus_tag="IV281_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8780079.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            31729..33510
                     /locus_tag="IV281_001049"
     CDS             31729..33510
                     /locus_tag="IV281_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8780080.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            33858..34544
                     /locus_tag="IV281_001050"
     CDS             33858..34544
                     /locus_tag="IV281_001050"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8780081.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            34564..38145
                     /gene="smc"
                     /locus_tag="IV281_001051"
     CDS             34564..38145
                     /gene="smc"
                     /locus_tag="IV281_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP8780082.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            38154..38963
                     /locus_tag="IV281_001052"
     CDS             38154..38963
                     /locus_tag="IV281_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8780083.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            38974..39972
                     /gene="ftsY"
                     /locus_tag="IV281_001053"
     CDS             38974..39972
                     /gene="ftsY"
                     /locus_tag="IV281_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP8780084.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            40159..42426
                     /gene="gshAB"
                     /locus_tag="IV281_001054"
     CDS             40159..42426
                     /gene="gshAB"
                     /locus_tag="IV281_001054"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP8780085.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(42515..42967)
                     /locus_tag="IV281_001055"
     CDS             complement(42515..42967)
                     /locus_tag="IV281_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP8780086.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            43117..44067
                     /locus_tag="IV281_001056"
     CDS             43117..44067
                     /locus_tag="IV281_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8780087.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
BASE COUNT        14682 a         7920 c         9609 g        11859 t
ORIGIN      
        1 caggatcagg aaaaaagaca ggagtattat aaatagaaga aacacgaaat aaaagagtca
       61 gtattttcta aatgaaactt tatgaaaaca aaatgttttt tacaagaaaa agccttgctt
      121 tgtgagttta tatatattat aatgacaaag gtgcgtttac tttagtgaat tgcacagacg
      181 taatcagaat gatttcgtcg gctaagaaac gagaggttgc gacacgcccg gacgctttgc
      241 cacgaacgag cgtgacggaa attttcgtgg agctatgtct acttttcaaa ataggcgaag
      301 gagggaacaa gatggcaaaa caaaaaattc gtatccgttt aaaagcgtat gaacaccgta
      361 ttttagatca atcagcggat aaaatcgtgg aaactgcaaa aagaactgga gctagcgtgt
      421 ctggtccaat tccattacca actgaacgca gtctttacac aatcatccgt gcgactcata
      481 aatacaaaga ttcacgcgaa caattcgaaa tgcgtacaca caaacgtctt atcgacattg
      541 tgaacccaac accaaaaact gttgatgctt tgatgaagct agacttacca tctggtgtaa
      601 acatcgaaat caaattataa aactaaaaca aaaataatgg aggtgtactc atgaccaaag
      661 gaatcttagg gaaaaaagtg ggaatgacac aaatcttcac tgaatctggt gaattaattc
      721 cagtaactgt tgtagaagct actccaaacg tagtactaca agtgaaaaca atggaaacgg
      781 atggctacga agctattcaa gttggttacc aagatatgcg tgaagtttta tcaaacaaac
      841 ctgcgaaagg tcatgttgca aaagcaaaca cggctcctaa gcgcttcatt cgtgaattca
      901 aaaatgttga gctaggagaa tatgaagtag gaaaagaaat tacagtagat gttttccaag
      961 caggagacat tattgatgta acaggtacta cgaaaggtaa aggattccaa ggggttatca
     1021 aacgccacgg acaaagccgc ggaccaatgg ctcacggttc tcgttaccat cgtcgtcctg
     1081 ggtcaatggg tccagttgca cctaaccgtg tatttaaaaa caaacgtctt gctggccgta
     1141 tgggtggcaa ccgcgtaact atccaaaact tggaagttgt acgtgtcgat gcagaaagaa
     1201 atgttatctt gatcaaaggt aatgttcctg gagctaaaaa atctttaatt actatcaaat
     1261 cagctgtgaa agctaaataa ttgtaggaag aaaggaggaa ctaaggaatg ccgaatgtag
     1321 cattattcaa acaagatgga agccaaaacg gtgaaatcac acttaacgaa gaaatcttcg
     1381 gaattgaacc taatgaaagt gttgtttacg atgcaatcgt aatgcaacgt gcgtcattaa
     1441 gacaaggaac acacgcggtt aaaaaccgta gcgctgttcg cggcggcgga cgtaaaccat
     1501 ggcgtcaaaa aggaacaggt cgtgcacgtc aaggatctat ccgctcacca caatggcgtg
     1561 gaggtggcgt agtctttgga ccaacaccac gttcttacag ctacaaactt cctaaaaaag
     1621 ttcgtcgttt agcaatgaaa tctgtattgt cagaaaaagt tgctgaaaac aacttggtag
     1681 caatcgaagg cttgaacttc gacgcaccaa aaacaaaaga attcaaacaa gttcttgcta
     1741 acttatctat cgactctaaa gtattggttg ttctagaaaa aggtaacgac tttgctgctt
     1801 tatcagcacg taacttacca aacgtttctg tagtaacttc tgataatgtg agtgtgctag
     1861 acgttgtatc aaatacaaaa gtgttagcaa cacaaactgc tcttactcaa attgaggagg
     1921 tgcttgcata atgaacttac tagacgtaat caaacgccca gtgatcactg aaaaatcaat
     1981 gcttgccatg gatgacaaga aatacacttt tgaagtggac actcgcgcaa acaaaacttt
     2041 agtaaaacaa gcggtagaag ctgcttttga cgttaaagtc aaaaacgtga acatcgtgaa
     2101 cgtacgtgcg aaattcaaac gtatgggcaa atatgcagga tatacaaaaa aacgtcgcaa
     2161 agcgatcgtt acattgactg aagattcaaa agaaatccaa ttattcgaag ctgctgaata
     2221 agcactaata gataactcat taaattagga gggaaaaacg tggcgattaa aaagtacaaa
     2281 cctacctcaa atggtcgtcg taacatgaca ggttctgatt ttgctgaaat cacaacatca
     2341 acacctgaaa aatcattgtt acaaccattg aaaaaccatg ctggacgtaa caacaacggc
     2401 cgcatcactg tacgtcatca aggtggcgga cacaaacgcc aatatcgcgt gatcgacttc
     2461 aaacgtaaca aagataacgt tgttgcaact gttcaaacga tcgaatatga tccaaaccgt
     2521 tcagctaaca tcgcgttagt acactatgaa gatggagtga aagcttacat cttagcacct
     2581 aaaggactag aagtgggtat gaaacttgtt tcaggtccag aagcagatat caaagtcgga
     2641 aatgctcttc cattagaaaa cattccagtt ggtactgtta tccacaacat tgaaatgaaa
     2701 cctggtaaag gtggacaatt gatccgttct gctggtacaa gtgctcaagt acttggtaaa
     2761 gaaggcaaat acgtattgat ccgcttgaac tctggtgaag ttcgtatgat cttagctgca
     2821 tgccgtgcta caatcggttc tgtaggtaac gaacaacacg aattaatcaa catcggtaaa
     2881 gctggacgct ctcgttggat gcgtaaacgc ccaactgtac gtggtagcgt aatgaaccct
     2941 aatgatcacc cacacggtgg tggtgaaggt aaagctccta tcggacgtaa agcaccagtt
     3001 tctccttggg gtcaaccagc tcttggttac aaaactcgta acaaaaaagc taaatcagac
     3061 aaacttattg ttcgtcgtcg caaaaataaa taattacttt gaccatgtgt cgcacatttt
     3121 gagaggaggt tcaccatggg tcgtagttta aagaaaggac cttttgtcga tgagcattta
     3181 atgaaaaaag ttgaagctca agcaggggtt gaaaagaaaa aagttatcaa aacttggtca
     3241 cgccgttcaa cagttttccc aagttttgtt ggatatacca tcgcagttta tgatggacgt
     3301 aaacatgtac cagtttacat ccaagaagac atggtaggac acaaattagg tgaatttgca
     3361 cctacaagaa cttatcgtgg acatgctgct gacgataaga aaacgaaacg ctaatttgag
     3421 gggaggataa atccaaatgg cagaacaaat cacatcagct aaagcaactg caaaaacagt
     3481 tcgcacttca cctcgaaaag cccgtttagt aattgacctt atcagaggta aaagtgtcgc
     3541 agatgcaatt tcaatcttga aattcacacc aaacaaatct gctggaatca ttgaaaaagt
     3601 attgatgtca gcagttgcga atgcagaaaa taactttgac ttagatgttg aaaacttggt
     3661 agtaagcgaa gcttttgtta acgaaggacc tacaatgaaa cgtttccgtc ctcgtgcaaa
     3721 aggatcagct tctccaatca acaaacgtac aagtcatatc acggtagtcg tatcagaaaa
     3781 ataaggaggg ataatcagtg ggtcaaaaag tacatccaat tggaatgcgt gtaggcatca
     3841 tccgcgactg ggatgctaaa tggtatgctg aaaaagagta tgctgagttc ttacacgaag
     3901 atttaagaat ccgtaaattt atttcaacta aacttgctga tgctgctgta tcaacagttg
     3961 aaatcgaacg cgcaacaggt cgcgtaaaca tttcaattca cacagctaaa ccaggtatgg
     4021 ttattggtaa aggcggttct gaagtcgaaa gcctaagaaa agaattaaac aaattaactg
     4081 gtaaaagaat ccatatcaat atcgtggaaa tcaaaaaacc agatctagat gcaaaattag
     4141 taggtgaagg aatcgcacgt caattagaaa accgtgttgc tttccgtcgc gctcaaaaac
     4201 aagccatcca acgctcaatg cgtgctggcg ctcaagggat caaaactcaa gtatctggtc
     4261 gtctaaacgg agcagatatc gctcgttcag aaggatactc agaaggaact gttcctcttc
     4321 atactttacg tgcggacatt gattacgcat gggaagaagc agatacaaca tacggaaaac
     4381 taggagttaa agtgtggatc tatcgtggag aaattcttcc aactaaaaaa aacactgaga
     4441 aaggagggaa ataatcatgt tagtacctaa acgtgtaaaa caccgtcgtg aattccgcgg
     4501 aaaaatgcgc ggggaagcta aaggcggaaa agaagtagca ttcggtgaat acggtttgca
     4561 agctgttgat tcacattgga tcacaaaccg ccaaatcgaa gctgctcgta tcgcaatgac
     4621 tcgttacatg aaacgtggtg ggaaagtatg gattaaaatt ttccctcaca aatcttatac
     4681 tgccaaagca attggggtac gtatgggttc tggtaaaggg gcacctgaag gatgggttgc
     4741 accagtaaaa cgtggtaaaa tcatgtttga aatcgcaggc gttcctgaag aagtagctcg
     4801 tgaagcgtta cgtctagctt ctcacaaatt accaatgaaa actaagatcg taaaacgtga
     4861 ggaaatgggt ggtgaatcga atgaaggtta aagaaatcag agaattaacc actgccgaaa
     4921 tgcttgatca agaaaaacaa ttaaaagaag aattgtttaa tcttagattc caattagcaa
     4981 caggtcaatt agaaaacact gcacgtatta aagaagtacg taaatcgatt gcacgcatca
     5041 aaactgtgtt gcgtgaacaa gctaagtaat tatggaagga ggccattaag cgtatgactg
     5101 aaggaagaaa tcaacgcaaa gtttatcaag gtcgcgtggt ttcagacaaa atggataaaa
     5161 caattaccgt tgctgtcgaa acaaagaaaa accaccctat ctatggcaaa cgcatgaact
     5221 attctaagaa atacaaagtg catgatgaaa acaacacagc aaaagttggc gacatcgtga
     5281 gaattatgga aactcgtcca ttatcagcta caaaacgttt ccgtttgttg gaagtagtcg
     5341 aagaagcagt tattatctaa taacacgaga ttttcctata tagattgaat gcaaaatctg
     5401 aaaggaggat acacaacgtg atccaagcag aaagtcgttt aaaagtcgct gataactcag
     5461 gcgcacgtga aattttgacg atcaaagtcc ttggtggatc tggtcgtaaa actgctaaca
     5521 tcggtgatgt gatcgttgct acggttaaac aagcaacgcc aggtggggtt gtcaaaaaag
     5581 gtgacgtcgt taaagccgtt atcgttcgaa caaaatcagg agctcgtcgt acagacggtt
     5641 catatattaa atttgatgaa aatgctgcag tgattattcg tgatgataaa agccctcgtg
     5701 gaacacgtat ctttgggcca gttgcacgtg aactacgtga aaacaacttc atgaagatcg
     5761 tttcactagc accagaagtt ttataatctt gagacactta tcaaaggagg tgcgaaacag
     5821 ttatgtttgt taaaaaaggc gataaagtta aagttatcac tggaaaagac aaaaacaaag
     5881 aaggcgttgt actagcagcg ttcccgaaaa aagataaagt tattgtcgaa ggtgtaaatg
     5941 tcgttaaaaa acaccaaaaa ccttctcaag ctgctcctca aggtggaatt gttgagatgg
     6001 aagcgccaat tcatgtttct aacgtgatgg tggttgactc tactggtgta gctggccgag
     6061 ttggctacaa agaagtagac ggcaaaaaag tccgtgtttc taaaaaaacc ggtgaagttt
     6121 tagataaata aaagtttaag gaaggagggt cttactgaat gaaccgccta aaagaaaaat
     6181 atcttaaaga agtaactcca tcattgatgg aaaaatttaa ctatagctct gttatgcaaa
     6241 cacctaaagt tgaaaaaatc gtcatcaaca tgggtgttgg tgatgcggta tcaaacgcta
     6301 aaaacttaga taaagcagtt gaagaattaa ctttgatcac tggtcaaaaa ccaatgatta
     6361 caaaagctaa aaaatcaatc gctggattcc gtttacgtga aggaatgcca attggtgcaa
     6421 aagttacatt gcgcggagaa agaatgtacg aattcttaga caaattggtt tctgtttcac
     6481 taccacgtgt acgtgacttc catggtgtaa gtaaaaaagc ctttgacggt cgtggaaact
     6541 atactttagg tattaaagaa caattgatct tcccagaagt agattatgat ttagtagata
     6601 aagtacgtgg tatggacatc gttattgtaa caacagcaaa ctctgatgaa gaatctcgtg
     6661 agttgttgac acaattaggc atgccattcc aaaaataatt aaaggaggcg aactacgtgg
     6721 ctaaaaaatc aatgattgct aaaaacaaac gtcctgcaaa attttcaaca caagcttata
     6781 ctcgttgcga acgttgcgga cgtccacatt cagtttatcg taagttccat ctttgccgta
     6841 tttgcttccg cgaacttgcc tataaaggtc aaattcccgg cgtgaagaaa gctagctggt
     6901 aaaaagtaaa ttcgcataag aaggaggtaa aagatcaatg gtcatgacag atccaattgc
     6961 agattttcta actcgtatcc gtaacgcaaa catggttaaa catgagtctt tagaagtacc
     7021 tgcatcaaaa attaaacgtg acatcgctga aatcttgaaa cgtgaaggtt tcgttcgtga
     7081 tgttgaatac atcgaagatg acaaacaagg cgtgatccgt gttttcctaa aatttggtaa
     7141 aaacggcgaa cgtgttatca caaacttaaa acgtatctct aaaccaggtt tgcgtgctta
     7201 tgtcaaagct gacgaagtac caaaagtatt gaacggttta ggtatcgcaa tcatttcgac
     7261 ttctgaaggt gttgtcactg ataaagaagc tcgtgaaaaa aatatcggcg gcgaagttat
     7321 cgcttacgta tggtaatcaa gtagaatcta caaggaggtg tctttaagtg agccgtatcg
     7381 gaaataaagt cgttgttatc cctgaaggag taacagttac tcaagacgga aacaacgtta
     7441 cagttaaagg accaaaaggt gaattgactc gtactttttc agcagatatc aaaatgaata
     7501 tcgaaggaaa cgaagtaact ttcactcgtc caaacgacag taaagaaatg aaaacaattc
     7561 atggaacaac tcgtgcgaac ttcaacaaca tggttgtagg tgtcagcgaa ggtttccaaa
     7621 aagcgttaga actaatcggg gttgggtacc gtgcacaatt acaaggatca aaacttgtat
     7681 tgaacgttgg ttactcacat ccagtagaaa tcacaccacc agctggagta actgttgaag
     7741 taccagctaa cacacaagta attgttaaag gtgcgaacaa agaagaagtt ggcgaattag
     7801 ctgcaaacat tcgtggaact cgtcctccag aaccttataa aggtaaaggt attcgttatg
     7861 ttggcgaata tgtacgccgt aaagaaggta aaactggtaa ataatcgttt agaaaaattg
     7921 catagaagcc agcagcagga aaaaggcatt tggctgaagt ttaccttcaa aaaaatacat
     7981 ttttacttgt gccgcagctt ctatttttcg aaacgtattt actgcttgaa atagtagtac
     8041 aataaaaata aagaggtgac aattgtgatt acaaaaccag ataagaacaa aacacgtcaa
     8101 aagagacatc gtcgtgtacg taacaatatc tctggtactg ctgagcgccc acgcttgaac
     8161 gtttttcgtt ctaacaaaaa catctacgct caaattattg atgacgtagc gggtgtaacg
     8221 ctagcaagtg cctctacctt ggataaagaa atttcaggtg gaacaaaaac agaagcagca
     8281 caagctgtcg gtaaactagt tgctgaacgt gcagctgaaa aaggcattaa agtagtagtc
     8341 tttgaccgtg gtggatacct ttaccatggc cgtgtgcaag ctttagctga agctgctcgc
     8401 gaaaatggac tagaatttta gaagaaggag gaataccatt catggtttat atcgatccaa
     8461 aacatttgga attagaagac cgcgttgttg caattaaccg tgtaacaaaa gttgttaaag
     8521 gtggacgtcg tctacgtttt gctgctttag ttgttgtcgg tgacaaaaac ggacacgtag
     8581 gatttggtac aggtaaagct caagaagttc ctgaagcaat ccgtaaagca attgaagatg
     8641 cgaagaaaaa cttagttgaa gtacctatgg ttggttcaac aatcccacac gaagtaatcg
     8701 gtgcttttag tggtggtaga atcctaatga aacctgctgt agaaggttct ggggttgctg
     8761 ctggtggacc agttcgtgcc gtattagaat tagctggggt agcagatatc acttctaaat
     8821 ctttaggctc aaatacacca atcaacgttg ttcgcgcaac agttgaaggt ttgaaacaat
     8881 tgaaacgtgc agaagaagtt gcagaacttc gcggcaaatc agttgaagaa ttaatcggat
     8941 aaggaggaca acaataatgg ctgaattaaa agttacttta aaacgcagta ttatcggacg
     9001 tcctcaaaac caacgcgata cagttaaagc gttaggtctt acaaagatta acagttcagt
     9061 ggttaagcct tctaatgaag caattaaagg catgatcaat actgtttctc acctagtgga
     9121 cgttgaagaa atttaattta gaaaaatcat aactagaaca ttaaggaggt gccaaaccta
     9181 tgaaacttca tgaattaaaa cctgctgaag gttcacgcca agtacgtaac cgtgttggtc
     9241 gtggtacttc atctggtaac ggaaaaactg ccggacgcgg acaaaaagga caaaaagctc
     9301 gttcaggcgg cggtgtgcgt ttaggatttg aagggggaca aacaccattg ttccgtcgtt
     9361 taccaaaacg aggattcaca aacgtgaacc gtaaagacta tgcagtcatt aatcttgatg
     9421 tattgaaccg ctttgaagac ggagctgaag taactcctgt tgctttagtt gaagctggaa
     9481 tcgtgaaaaa cgaaaaagct ggaatcaaag tattagctaa tggggaacta aacaagaaat
     9541 taactgtgaa agcagcaaaa ttctctaaag cagctcaaga agcaattgaa gcggctggtg
     9601 gatcaatcga ggtgatctaa tgtttagact attaaaagat gcttttaaag taaaagacat
     9661 tagatcgaaa atcctattta ctgtatttgt gttgtttgta tttcgcctag gtgcacatat
     9721 tactgtacca ggcgtaaatg ctgcttcatt gcaaaacctt tcttctttgc catttttcaa
     9781 catgttgaac ttggtaagtg gtagtgcgat gcaaaacttt tccattttct caatgggggt
     9841 ttcaccatac attactgctt ctatcgtcat tcagcttctt caaatggaca tcgtgcctcg
     9901 atttgttgaa tggtcgaaac aaggggaagt tggtcgtaaa aaactgaatc aagcgactcg
     9961 atatctgaca ttagtattgg catttatcca atctgtcggt atcactgctg gattcaatca
    10021 atacgctcaa ttaggtttcg tgaataatcc taacgtagca acttacgtaa cgatcggttt
    10081 gattctaaca gccggtacga tgtttgttac ttggctagga gaacagatca cagaaaaagg
    10141 tatcggaaat ggcgtatcaa tgatcatttt tgcaggtatc atttctagat tgcctgaaag
    10201 tatcagagaa atttatgaag attactttat aaatattgat ccttcagaca tatggaaatc
    10261 tgctattttt gtggccattt tactagtagc ggtcttgttg atcgtgacat tcgtaacatt
    10321 tttccaacaa gctgaacgca agatcccgat ccaatatacg aaacgcgtag ctggcgcgcc
    10381 tacaagcagc tatctgccgt tgaaagtaaa tgcagctgga gttattccag ttatctttgc
    10441 tagttcgttt attgctacgc caaatgctgt tttacaagca ttgagcggta gattcggttc
    10501 tgaaaactgg tacaaagtga tgatggagat ctttaactat aatacagcac ctggtgctac
    10561 gatttatacg attctgatcg ttgccttcac tttcttctac gcctttgttc aagtaaaccc
    10621 tgagaaatta gcggaaaact tgcaaaagca aggaagttat ataccaagcg tacgccctgg
    10681 taaggggact gaagagtatg tctcaagatt actgatgcgt ttaagtacag ttggtgcagt
    10741 cttccttggt ttagtggcat tgcttccaat cattgctcaa atggtttggg atcttccaca
    10801 atcaatcgga ttaggcggta caagcttact gatcgttatc ggtgtagctt tagaaactgc
    10861 gaaacagtta gaaggcttaa tgctgaaacg taaatatgtt ggattcattg agtaagaaag
    10921 cgtgttgagg tttcccctca acacacgttc tcacacattt attaggaggg aaaacaatga
    10981 acctcatttt aatggggctg ccaggcgcag gtaaaggaac acaagctgaa aaaatcattg
    11041 atgcttatgg tattccgcac atctcaacag gagatatgtt ccgcgcagcg atgcaaaacg
    11101 aaacagctct tggcctggaa gcaaagtctt atatggataa aggtgcatta gttcctgatg
    11161 aagtaacaaa tggaatcgtg aaagagcgac tagctgaacc agatacagag aaaggcttct
    11221 tattagatgg ctttccacgt acgctagatc aagcaaaagc tctggacgca atgctaaaag
    11281 atttgaataa aaaaattgat gctgtcatcg atatccatgt tggtgaagaa atcttggtcg
    11341 aacgtctagc cggccgattt atctgcagca attgtggagc aacttaccat aaaattttca
    11401 accctacaaa agttgaagac acatgtgatc gttgcggtgg gcatgaattc tatcaaagag
    11461 aagatgataa acctgagacg gttaaaaatc gtctggctat caacatcaaa aatagtgaac
    11521 caatcttggc ttattataaa gaacaaggtt tgctaaacac tattgatggt gctcgtgaaa
    11581 tcgatgctgt atttgcagat gtacaaaaaa tcattgaaaa atagcacgat tgcccgtagc
    11641 cgacttgcat tatgcagtta ctcttgtcaa gttcaataaa ttatgataga atatgtcagt
    11701 ccgacttcta gagaatcctc tagatgatcc attttgagaa ttttgaggtg ggaactgcag
    11761 tttccgccat tttatcgtgt gaaaatgcga aacaagtaca aggaggtact gtgcgtggca
    11821 aaagaagata tgattgaagt cgaaggtaca gtcgtcgaaa ctttgccgaa tgcaatgttt
    11881 aaagttgaac tagaaaacgg acaccaagtt cttgctactg tttcaggtaa aattcgtatg
    11941 cattacatcc gtatcttacc tggtgacaaa gtaacggttg aattgtctcc atacgatctt
    12001 aaccgtggcc gtattactta tcgctttaaa taattgtact ccgtaaaact acaggaggta
    12061 ttatcatgaa agtaagacca tcagtaaaac caatgtgcga acattgtaaa gtaattcgtc
    12121 gtaaaggacg cgttatggtg atttgcccag caaatccaaa acataaacaa cgtcaaggat
    12181 aattggaggt gctataaata tggctcgtat tgcaggagta gatattcctc gtgataaacg
    12241 tgtagtagtt tcacttactt atatctatgg tattggtaac actactgcta aaaagatctt
    12301 agctgatgct ggtgtatctg aagatgttcg cgttcgtgaa ttaacgaatg aacaaacaga
    12361 tgctatccgt gcagaagtag ataaattaaa agttgaaggg gatcttcgtc gtgaagtgaa
    12421 cttgaacatc aaacgtttga tggaaatcgg ttcttaccgt ggaatccgtc atcgccgtgg
    12481 attgcctgtt cgtggacaaa acacgaaaaa caatgcacgt actcgtaaag gcccagctcg
    12541 cgctatcgct ggcaagaaaa aataattctt aagtgaagga ggttaaaact tcatggcagc
    12601 aaaaaaagtg aatcgtaaac gccgtgtgaa gaagaatata gaagcaggta ttgcacatat
    12661 ccattctaca ttcaataaca ctatcatcat gatcactgat actcacggaa atgcattagc
    12721 gtggtcatca gctggttcat taggtttcaa aggaagcaaa aaatcaactc cttttgccgc
    12781 tcaaatggca gcagaaactg ctacaaaagc agcaatggaa cacggattaa agactgttga
    12841 agtaacagtt aaaggacctg gttctggacg tgaagcagca attcgttcat tacaagctac
    12901 aggtttagaa gtgactgcaa ttcgtgacgt gactccagtt cctcataatg gtgcccgccc
    12961 tccaaaacgc cgtcgtgttt aatgagtgcc gctcattttg agttttatca gtaacgtcat
    13021 tgaacaagac acttttcgtt ttgaaagggg taaagataag aatgattgag ttcgaaaaac
    13081 caagaatcgc aaaaattgat gaagaaaaag attatggcaa gttcatcgtt gaacctcttg
    13141 aaagagggta tgggactact ttagggaatt ccctacgtcg tattttatta tcttctttgc
    13201 caggtgctgc catcactaat attcaaatcg atggtgtact gcatgaattc tctaccatca
    13261 aaggtgttag agaagacgtc actcaaatca tcttgaacat taaaggctta gcgctgaaaa
    13321 tgtatggcca agaagaaaaa acccttgaaa tcgatattac cggacctgct acagtaactg
    13381 ctggcgatat tatcgttgat agtgatgtag aaattcttaa caaagatatg tacatctgta
    13441 gtgtctctga aggagctacg ttccatgctc gcttaacagt gaaacctgga cgtggttatg
    13501 ttcaagcaga tgaaaataaa aaagaagata tgccaattgg cgttcttcca gtcgattcaa
    13561 tctacacacc tgttcgtcgt gtgaactatc aagttgagaa cacacgtgtt ggacaccgtg
    13621 aagattttga taaattgacg atggagattt ggacagatgg ttcaattgaa ccgttggaag
    13681 caatgagttt gtctgcgaaa atcatgactg aacatttgga tatttttgtg aacttaactg
    13741 atgaagcaaa aaatgctgaa atcatggtcg aaaaagaaga aacacaaaaa gaaaaaatgt
    13801 tggaaatgac gatcgaagag ttagacttgt ctgttcgctc ttacaattgt ttgaaacgtg
    13861 caggaatcaa tactgtacaa gaacttacaa ataaatctga gcctgaaatg atcaaagtcc
    13921 gtaacttagg acgtaaatca ttagaagaag ttaaagcaaa attgcacgac ttaggtttag
    13981 gtttacgtaa agaagattaa aaccttttac gaaggaggga aaccaacgtg agttatcgta
    14041 aactaggacg cacatctagc caacgtaaag cgatgttgcg tgatttaaca actgatttat
    14101 taatcaacga acgtattgtg actactgaag ctcgtgcgaa agaagttcgc tcaactgcag
    14161 aaaaaatgat cactttaggt aaacgcggag atttgcatgc acgtcgtcaa gctgcggctt
    14221 tcgtacgtaa tgaagttgcc agcgtgcgtg aagaaaatga ggacatcgtc attgaatcag
    14281 ctttacaaaa actgttcaac gatattgcac ctcgttacgc agaacgtcaa ggcggataca
    14341 cacgcatttt gaagacagaa ccaagacgcg gtgatggtgc accaatggtt gtcttagaac
    14401 ttgtctaaaa tcacactaat aacatcactt tatagatgat tcttttagag tgttatgatg
    14461 ttggaagata tttttccgag tctagctcag cgctgctcct caagcgaata tcgcttgggg
    14521 agtaagcact catcataaag tgttttttta taccttcaat tacctgtcat gacagatatg
    14581 tttgaaggca taaaaaaaca ttttaagcga gaaataattg aaaattttgt ctttttttga
    14641 tagtgttagt aggaacttta ggatgaagaa ggagtaatca tggagccaat tattgaatta
    14701 gaaaagataa attataaata ccagccagat gatcttcgcc ccgcattgaa agatgtctcc
    14761 tttacaattg ataaaggtga gtggatcgct attattggac acaatggttc aggaaaatcg
    14821 acacttgcca aaacgatcaa tggattgctt ctgccagaat cagggatcgt gaaagtggga
    14881 aatcaaatat tggatgaaga aaatatttgg acgatccgac agatggttgg aatggttttt
    14941 caaaatccag acaatcagtt tgttggttcg acagtagaag atgacgttgc ttttggtctt
    15001 gaaaatcaag ggattcccag agaagaaatg ctagtgcgtg tgaaagatgc acttgagaaa
    15061 gttagaatgg ctgagtttgc ttcacgcgag cctgctcgtt tatcaggagg gcaaaaacaa
    15121 cgtgttgcca ttgccggcgt ggttgcctta cgacctgata tcattatttt agatgaagca
    15181 accagtatgc tagatccaga aggtcgtgaa gaagtgatct ctacgatcaa gaagatcaaa
    15241 gaagaaagtc aattgaccgt tatatctatc actcatgata ttgatgaagc agctaatgcc
    15301 aatcggatac tagtgatgag acaaggcgaa cttgtccgtg aaggtacacc aaaagaaatt
    15361 ttttctgctg gtcctgaact gatcgactta ggtctagatc taccatttcc tgaaaaactg
    15421 aagagcgcac tgaaagaacg tggggttgat gtgcccagtg aatatatgac agaagaaagg
    15481 atggtggatt ggttatggac atccgttttg aacaagtaga cttcacttat cagccgaata
    15541 caccatttga acaaagagcc ttatttgaca tcaatatgac gatcaaagaa aacagttata
    15601 ctgcattagt agggcatacc ggaagcggga aatcaacctt acttcagcat ttgaatgcgc
    15661 ttgttaagcc aacaagcggt acagtccata ttggagaacg agatattcag ccggatacgg
    15721 ataataagaa tttaaagccc atccgaaaaa aagttggcat cgtctttcag tttccagaag
    15781 cacagctatt cgaagaaacg gtagcaaaag acattgcttt cggtcctaaa aactttggtg
    15841 tcagcgaaga agaagcacta gtcctagcaa aagaaacatt agaacaagtt gggctggatg
    15901 aaagctattt ggaacgttcg ccatttgaac tttcaggagg gcaaatgcgt agagtagcaa
    15961 tcgctggtgt gcttgccatg aggccggaag tacttgtatt ggatgaacca acggcaggac
    16021 ttgatcccca gggcagaaaa gaaatgatgg agatgttttg gcgtcttcat aaggaacagc
    16081 agatcactat tgtattagtt acgcatttaa tggatgatgt agccaatttt gcagattatg
    16141 tctatgtgtt ggaaaaggga cgaatcgtta atagcgggga gccgcaagag gtctttcaaa
    16201 acattgaatg gctaaaagaa aaacaactag gcgtacctac tgccaccgag tttgcagaag
    16261 aattaatggc taaagggatg aactttgcta ccttgccttt gacagctgaa gaattagcag
    16321 acgctatcgt ccattatgct ggaggaaatg cccatgatga ataaattgat atttggtcgt
    16381 tatatgcctg gagattcctt gatccatcga ttggatcccc gagctaaatt attagccagt
    16441 ttttatttta tcggaatcat ttttttagca aataattggg aaacgtacct tttgttggca
    16501 tttttcacat tactttctgt cttcttgtca aaagtagatt tgaaattttt tatccgaggc
    16561 attcgcccat tgatctggct aatcctcttc actgtagctt tgcaggtctt gtttacaagt
    16621 ggcggagaaa cttattggag ttggggtgtt ttccatatca ctgaatttgg tgtgatgaac
    16681 ggcctcttta ttttctgtcg attcgtgttg atcattttta tgtcgactct actcacgctt
    16741 actacaccgc ctttggaatt atcggatgcg attgagtact tgcttcgtcc attgaaagcc
    16801 gtacggttcc cagtccacga aatttcattg atgctgtcga ttgctcttcg ttttgtgcct
    16861 acattgatgg acgaaacaga aaagatcatg aacgctcagc gcgctcgcgg tgttgatttt
    16921 ggtgaaggaa acctagttca gaaaatgaaa gcagtcattc ctttgctgat tcctttattt
    16981 gtcagcagct tcaaccgtgc ggaagatctt gcgactgcta tggaagcaag aggctacaaa
    17041 ggtggggaag gaagaacgaa atatcgtgta ttgcattggc ataatagaga cacgatggtt
    17101 atcattgctt ttgtattatt aactgtgcta ctgatctttt taagaggata aacgttcagt
    17161 gggccgctat ctattctttt cttgctgatc tgtttgcaag aaaggatgca agcagtgtat
    17221 gacaagaaat attagggtct gtggcagact aaggtcacag gcctctttct tttatctgaa
    17281 aagaaagaac gaaaaaattt tatagaaaaa tcattattcc agataacctt ttaggaacaa
    17341 aggagagaaa aacatggtac gttacaaagc aatcattgcg tacgacggaa caaattttaa
    17401 tggattccaa aaacagccaa atgggcgtac tgttcaagaa gaagtagaaa aaacattaca
    17461 aaaaatggca aatggaaaag aaatcacggt ctttggttcg ggaaggacag atgccggagt
    17521 acatgcgatc gggcaagtaa tccattttga ttatccagag gagcgtccgt tagaacgaat
    17581 gcgtttcgcg ctagataccc agtcaccaga ggatattgct gtaagacaag tagaaattgt
    17641 ttcagaagac ttccacgcaa gatatttggt caaagaaaag acgtatcaat ttcgagtgga
    17701 tatagggaaa ccacgcagtc cttttcgacg ccattatgca agttattttc cttacccact
    17761 tgatttggaa aaaattcagc gggcgcttcc tgatttatta gggactcacg atttcacctc
    17821 tttttgtgca tcaggaagct cgatagagga taaaatacgt acgatctacg aggctaagat
    17881 ggaggtaaat gaaacaaaag acgaattgtt gtttacattt aggggaaatg gcttcttgta
    17941 taagatgata cggatcttag taggaacgct tttgaaaatt ggcaatggcc gattgcctga
    18001 agatagtatc ccagatatta ttgcgaaaaa agatcgtaat gcagcaggac ctacagcaca
    18061 ccctgaagga ctttatttgt atgaagtgaa atatgaataa gaggttatga tagaagtggt
    18121 cagcttcaag aaataagaag gtatttacga aaattacttc tcaaaatttt gcaaaatttc
    18181 ggcttatttt cgaagaagct acttctggaa caccgtttaa ggaagaggtc gtgaaatcag
    18241 cgttttgacc tgagtattag agaaacaaac gggaaaatag ctcctcatat tttgccgttt
    18301 gttgagctaa taccgaaggt cagctgattg aacaccgttt aagaaagagg tcgtgagcct
    18361 gtcatttaac tctgagtcac aaagagcaat ttcaaaaaac agcttctcat ctttgatgaa
    18421 tatcgtgact tgtggacgag gcgttgataa gtgaatcatc tttttgttac ctattttttc
    18481 tgtaaccaaa aaacgatgga acgttgtttt gattgccctt ggtgcggaca gtctgaacag
    18541 gttccatcgt ttctttattt tctacgtctt cttctgtgtg tgtttcgtgg ttgttcttct
    18601 attgctggat caatcgtgcc ataaaagggg cgattgcggt caaggtacca gacataaaga
    18661 ataaacaatg caatacatag gacaaaaagg attgcaccta tcgtaaatcc ttcaggcaag
    18721 taactgaaag tgacagtatg tttacctgct ggaactttta tcatcaagaa tgcgtctttg
    18781 aaattgctga ccttgactgg tttcccatca attttagctt tccatccttt gtcataagga
    18841 atagttgtca ccaacatgga atcttcagcg gtatttacag tcccagaagc ttttctgcca
    18901 gtggcttcca tttcgacacc tttttctttg atctgattca ttgcggcttg ataggctaca
    18961 acatctagac caacgacttt aggctccatg aaacttattt ctttcgtccc ataaaaactt
    19021 gcagtaaatg tcactgttgt ctcttgagga taatagccaa gatcatagta ttgaccagaa
    19081 atgttgattt gcgtttttcg cgatgtacca ttgacagtga tcgtcgcact agaactttct
    19141 aattgcgcaa aattggttgg gaacaaactt aagtaagctt gtgtatttgc aggcacatgg
    19201 acttcccaag tgagctcttt tggctggtta tctgctgttt ctgagtaagt gacgccagtc
    19261 gttgtctttg tgatcgtcgt attcaactga ttcttcaaag taggtgtata gaatgtaaaa
    19321 tactgttcat ttagaccaga caatgtattg atcaagtttg tctgactggt caaattatct
    19381 gtcactggct gctcaacatc attaacggac aacggtgcag taaatgctag tggcaatgca
    19441 ttgacattct gatacagtgt atagtcgccg ctttgaccag cttggcggaa gccatattta
    19501 ctgaacgtgc tttcgttttt agaaatgtta tatttgattc ctgtaaaccc atccatcagc
    19561 aaagtattat tgttataacg gatattcaaa tttgtccctc ttgaacggaa acctaattta
    19621 tccaggtaag aggaagaatt acgatttctt atagaagaaa acaaactgat accactgtaa
    19681 ccatagttga tcgaatcgtt tgcagatact ggatccagat tttccaagcg gtaaaacgag
    19741 tcggaatctt tttttgtttc atttacaaga gctttcaagt caggataagg atcagtatat
    19801 aaactgcggg aagcataatt ccaatcatca agaatgccat ggatcatact gttcgtattg
    19861 accagtgctt cagcagacat gatcaataat aacaatatac tcagatgatt gaatgaaagt
    19921 tttttatgct gataaaatcc cacagcaaaa gcataaatcc ctaagaaaac cactgtcaat
    19981 acaaaggaag tcatcgtcac ataggtgtag cttccttcag gtttgatgcc ccatgccacc
    20041 gcaaagagag cagctacgac caaaatgatt cctaacaaca gcccacgatc gtcttgttct
    20101 atcttttccc atccttttcc tgcaagcagg agcactaaga aagagaacag ataggcataa
    20161 cggaataaaa acatattcgg tgcatgcata ccatgccaga acaaattcaa tggaacaaga
    20221 taaaaacttg cgataagtat ggcgaaaaga ctgccgtaca aaaatttatt tttcaacggt
    20281 actttccgac tggcaaaata aaacagacag aggactaatg gtagcagacc tgcgtaaacg
    20341 aacggaatcg atccgtattt tgtcgtgtca taaacaccaa tcatattttt catgattaga
    20401 tccagataag aagtcgcttc tgttttcaac gtagtgatct gactcagtgc ctcgccattt
    20461 gaccgaagat ccagcactgc tggcaataca atgatcattg aagcgccgcc tgccagtaaa
    20521 gaagtgatac cataaggaac aatcgttttt ttatattctg accagtttgt caatagacgg
    20581 gcgataaaat ataagaaaga aaatacgcca atcataaaac ccatataaaa actggataaa
    20641 aacaagagta aataactgat aaataaaagt ttcggccgtt taagatccat aacacgatga
    20701 atccccaaaa tcactaatgg aagataaaaa aatgcatcca gccacatgat gatctccgaa
    20761 tgtgcagtga taaatgagat caatgcgtaa ctgacgctaa gtgcaacatg cccccagcga
    20821 ggaatcttga acgtacgtgc aagaaaccaa aaagccaatc cggcacagcc gaccttaagc
    20881 aaagtcagaa catacagtgc atctggcatc atctggtttg gaaaaaagaa caccagtggc
    20941 gtgaagatcc cgcctagata gtaagaaatt aaagataaat aattcaatcc tagagaagca
    21001 ttccatgtat aaaaaatact ttgctttcca agaagaacat ttcggaagct ggcatgaaaa
    21061 ttagaaaact gtgaaaaggc atcacttgcc aaaatacttc tgctgcttcc aggatagatt
    21121 ccgatactta gataaacaag tgctaaaatc aaaaacggaa tcaggaaact agctgctgcg
    21181 tagcgtccgt tttctctaaa gaatgacttt aaattttttt tcatatttat tttccaccaa
    21241 atttctatga agttgtattc agtttatcat atccttgttt ttctctgctt aaaaagagtc
    21301 tagttttcac aaaaaaataa aatttagaat aaataacttc ataatttgtt cgtataacaa
    21361 aaataaacgc aaaaaaaggg taggaatcaa aagtcccacc cttaaaaatt tttattaaat
    21421 aaaaaaccgt tcactatctg aacggtccaa gtatcattca attaaacgcg ttcaactttt
    21481 cctgatttca aagcacgagt tgacacccaa acttttttag gttttccgtc gaccataacg
    21541 cgaacttttt gtaagttagg tttaacagta cgttttgttg agttcatagc gtgtgaacgg
    21601 ttgtttccac tttttgtctt gcgacctgta aagtaacaaa cttttgccat ggtaatttcc
    21661 tcctttgcct tgatttggag caatagaata attcagctca taccagacta acatatcata
    21721 tagaatggga atttgcaagt acattttcat agatacaatt gacaattgag aagaataatt
    21781 gtaaattaaa caaggttata cctgaaattt tgcaaggttt ttgagtactt tgggtaactg
    21841 ccttttattt caagttgcgc tatgataaaa tatgatagaa gaaaaatggg agttttttac
    21901 ccgttaagga ggctcattca tggctgtaaa aattcaaacg tcagctggaa ccattgaaat
    21961 taccaatgat gtaattgcaa cagttgttgg tggcgccgcg acagatgtgt tcggtgttgt
    22021 aggaatggcc agcaaaaatc aaattaaaga taatattact gagattttgc gtaaagaaaa
    22081 ttactcaaga ggagtagttg tccgccaaga agataatggt attgctgtag atgtttatac
    22141 gattatcagc tatggtacga aaatctctga agtttcccgc aacgtccaag aaaaagtgaa
    22201 atacaatcta gaggcattat taggcgtaac tgccaattca gttaatgtct ttgtacaagg
    22261 tgttcgtgta ctgccagact aggcaggaga ctggctggta actcagcagt cgatctgaag
    22321 gaggattatc taggtgagtg taacagaaat aagtgcaagc cagttccagg aaatggtcca
    22381 ggcaggtgcg aatcgtctgc aagtaaatgc agaatacgtt aattcattaa atgtattccc
    22441 tgtgccagat ggcgatacgg gaacaaatat gaatctatct atgactagtg gagctaaggc
    22501 agtagtagat tcaacctcag aaaaagtagg agaattagca gcaatgcttt caaaaggatt
    22561 gctgatgggt gcgcgcggaa attccggcgt tattttgtct caattgtttc gtggattttc
    22621 taagcaaatt ccagatgttg tctcattaaa tgcttctgat cttgctgcag ccttcactca
    22681 tggagttgaa actgcgtata aagcagtaat gaaaccagta gaaggaacga ttttgacagt
    22741 agctagagaa gcagcgaaag ctggagaaaa gaaagcaaaa agtactgatg atgtcgttga
    22801 agtaatgaca gctgttgtaa aaggagcaaa gcgtgcttta gctaaaactc cagacttgct
    22861 tccagtccta aaagaagtcg gtgtcgtaga cagtggaggg caaggactgc tgtttgttta
    22921 tgagggcttt ttaagtgcat tgaatggaga attccaagcg gatgaaacat atgaaccttc
    22981 tccagcagaa atggatgata tggtcaatgc agaacatcat cgcagtgtcc aaggtcaaat
    23041 ggcgacagaa gatatccatt ttggttactg tacagaaatc atggtaaaaa tcggagaagg
    23101 acctacagtc gatagtacat tcgattacga agaattccgt aactatttag atggtatagg
    23161 agattcttta cttgtggtca atgatgacga gatcatcaaa gtccacgtgc atacagaaaa
    23221 cccaggagaa gtcttgaatt atggacaaaa attcggttca ttagtcaaaa tcaaagtcga
    23281 caacatgcgc ttgcagcatg agacgatttt ggaacacgat gaagaagtga acgagtttgc
    23341 gaaaaaagaa ataccacgta tcccgtatgg tatcgtagcg atcgctgctg gtaaaggcgt
    23401 ccaagagctg tttgaaagtt taggcgctaa ttatgttatc agtggtggac aaacaatgaa
    23461 tccaagtacg gaagacattg ttaaagcgat tcaagaagta aacgcagata aagtgattgt
    23521 cttgccaaac aataagaaca tcttcatggc agctgatcaa gcagcagaag tagcagatat
    23581 cccagtagca gttgttcctt caagaacgat ttcccaagga ttgacagcta tgctttcatt
    23641 caatgatcaa gcttctttag aagaaaacaa agaaacgatg acagaaatgt tgtctagcgt
    23701 tgtgagcggt caagtcactc acgcgatcag agatacctca attgacggtg tagagatcaa
    23761 agaaggagac tttttgggca tgatcgatgg aaaaatcctg atctctaacc cagatatcac
    23821 agcaacagcg ttaagtacac tagaacaaat gatcaatgag gacacagaga tcgtaacgat
    23881 cttggcaggt gatgaaggaa cagttgaaca agcaaatgaa tttgctgaga aactaacaga
    23941 aaaacatcct gatttggaaa tcgagatcca ccaaggagat cagcctgtct atccttatct
    24001 attttctgct gaataatttc tgtgtcagaa tatctttcta acacagtatt cattcataaa
    24061 aggccttcgg ttagtgatca ggtatgatcg ctgaccaagt cctttttttg ttaaaggagg
    24121 gaggaaaaat ttatgcgttc tttagatgat ccaatcacta tgctggcagg agtcggagaa
    24181 aaaagagcag ctagtcttgc cagcttgggc atccaaacga ttgaagactt actgacttat
    24241 tatccttttc gctacgaaga tattcaggaa agaaatctaa acgagatcca ggatcaagaa
    24301 aaggttactt tgaaaggaat cgtagtctcg cctcctgtga tgaatcgatt tggttataaa
    24361 aaaagccgat tgcaatttcg tatgatgcaa gaacatgatg tgttcaacgt ttcttttttt
    24421 aatcaacctt atctaaaaga aaaagtcatt ttatcggagg aaatcgccgt atacggtaaa
    24481 tgggatgcaa agcgtaaagc cttgaacggg atgaagattt taggttctca aacaatggat
    24541 gacttttcgc cgatatatca cgtcaacaaa tcaattcgtc aaacgacatt agttgatcta
    24601 atcaaaaaag ggtttcaaga atacggcaca ttgattgaag aaaatctgcc aaacaacctt
    24661 gtggagaaat atcgactgct tgaccgagct tctgctgtaa gaagtatgca ttttccaaaa
    24721 gaccacgaag aaaaccatca agccaaacga cgtgtcgttt ttgaagagtt ctttttattt
    24781 caaatgcgtc ttcaaggatt gaaaaaagca gaaaaagcag aaacgaatgg tttggagatc
    24841 ctttatgatg tccaaaaatt gaaacaattt actcaaaagc taccttttga actgacaaat
    24901 gcacaaaaac gtgtaacgaa tgaaatctgt cgggatcttc gcagtccgca gcatatgcag
    24961 cgacttttgc agggagatgt aggtagcgga aaaacagtgg ttgccgcgat tgcgttatac
    25021 gctaccgtta cagcaggttt ccaaggtgcg ttgatggtgc ctacagaaat cttggcacaa
    25081 caacatatgg aaagcttgca acagctgttt gatcccaatg aagtaaccat cgcattgatt
    25141 acggggtcaa cgaaagcaaa agaacgacgt gagctccttg aaaaactaga gcaaggggaa
    25201 attaacgtcg tgatcggcac acatgcattg atccaagatg gagtcgaatt tcagaattta
    25261 ggtctagtta ttacggatga gcagcaccgt tttggcgtga accagcggaa agtacttcgt
    25321 gaaaaaggct ggcgaccaga tgtcttattt atgacagcga ctccgattcc tagaacatta
    25381 gctattacag catatggtga gatggatgta tctatcattg atgaacttcc agctggacgt
    25441 atcccgatcg aaacacgctg ggtaagaaca cctcaattgg acagtgtact ggattggacg
    25501 aaaaaagaac tggcaagagg acatcaaatg tatgtcatct gtccgctgat tgaagaatca
    25561 gaagcattag atgtaaaaaa tgcagtggaa atctatgaaa aacttcgtga actatttgca
    25621 ccaaaatatg aagttggctt gttacatggt aaaatgaaga atcaggaaaa agaagtaatc
    25681 atggaagcct tcaaagacaa ccagatgcag atattagttt cgactactgt aattgaagta
    25741 ggggtaaacg tgccgaacgc gaccgtcatg ctgatcatgg atgcggatcg atttggattg
    25801 gcacagctgc accaacttcg tggacgtgtt ggacgcggaa gtgatgcttc ctactgtatc
    25861 ttagttgcta atcctaaaaa tgaactaggt gtggaacgga tgaaaatcat gacagagacc
    25921 accaatggtt ttgtactaag cgaaaaagat ctggaattac gtggtcctgg tgaagtattt
    25981 ggcttccgtc aatctggtct gcctcaattt gcgattgctg acttggtgac ggatggaaat
    26041 gttttggagg tagccagaga agaggctgca gccctttggc aaataaagga ttggcagatg
    26101 cttcctgagt atcagccttt agcgcaaagc ctccaaatag aaaatcagga taatcgttat
    26161 ttcgattagc agactgtgct atactttata ctgatgacgg aaaaaggagt ggaaacaatg
    26221 aaaattgccg tagatgcaat gggcggagat cacgcacctc aagcaattgt agaaggcgtg
    26281 gctttagcgc agcaagattt tccagaagta gaattcttac tttacggaaa agaagcagaa
    26341 atcaagaaat acttaacaaa tgaaaaaaac atcacgatta tccatacaga cgaaaaaatc
    26401 aatagtgatg atgagccagt aaaagcaatt cgacgaaaaa agactgcatc aatggtcctt
    26461 gctgcacaag cagtgaaaaa cggtgaagca gatgctgtat tttcagcagg gaatacaggc
    26521 gcgctactag cagcagggtt gttcatcgta ggacgtatca aaaatatcga acgccctggc
    26581 ttgatgtcta ccttgccagt agtagggaaa gaaggtgccg gtttcgatat gctggatctt
    26641 ggcgctaacg cagaaaacaa accagaacat ttgttgcaat acggtatttt aggttctttt
    26701 tatgcacgta aagtccgagg tatcggacgt cctcgtgtcg cactcttaaa caacggaaca
    26761 gaagcaacaa aaggtagtga agtgacgaaa aaagcctatg aactccttca gaatgaaaca
    26821 agcttgaatt ttattggaaa cgtagaagct cgtgagctac tcaatggtgt ggctgatgta
    26881 gtggtcacag atggatttac aggcaacgct gttttgaaat ctattgaagg aacagcaatg
    26941 aatatgatga atctattaaa atctgctatt ctaaatgaag ggatcaaagg aaaaatgggc
    27001 gctatgcttt taaaagacgg cttacgttct ttgaaagcag aaatggatta ttccaaacat
    27061 ggcggtgccg ttttgtttgg cttgaaagca ccagtcatta aaactcatgg agcaacaggc
    27121 cccgatgctg ttcgctatac gatccgccaa atccacacaa tgcttgagac agatgtagtt
    27181 ggacaattag tcgaacaatt tgaaaaaagc gaagaatagt atttggatat ctgaagcgat
    27241 ccgttccatt gaaaaaaagg tggacattcc tgaaaaatgc cagtaaaatt agccatggtg
    27301 taggtaattg acaagtgtat gattcttgga ggtgaaaaag ttgactcgcg aagaagtatt
    27361 taacaaagta gcgaaaatta tttcaaatca ttttgagata gataccgata aagtgacgga
    27421 tgaattgaac atcaaagatg atttaaaagc tgattcaatt agtattatgg aattcgtttt
    27481 agagcttgaa gatgaattcg gtacggaaat ttcagatgaa gacgctgaac aaattgaaac
    27541 agtcggtggc gcagtagatt atatcagcag ccatcttaaa taatttcaaa taaaagcaaa
    27601 gaaggataaa aagctgcatg cagcttttta tccttctttt tttaatcttt ttgacacgta
    27661 aaagtcattt ttctgataat tctggcgata aacagaaatt atgttgcaaa gtaaagcggt
    27721 tttctatata attattaata attcattaca caaagataag aataaccttt gttataaaac
    27781 gatttattct gtgtaatgtc cttttttatt aagaaaaact tccatttagg aagcagagag
    27841 gggtattcga ttgtccgata accaattatt agaagttcag aacctacata caggattccg
    27901 actcaaagat gactattacg atgcagtaga tgacgtttca tttactttag acaaaaacga
    27961 gattttagca atcgttgggg aatctgggtg cgggaaaagt accttggcta ctacgattat
    28021 gggattactt gatcctaata acacaaaaat tacaggtgag attttatata acgatctgaa
    28081 tttaatcgac ctgaacgaaa cgctctataa taaaatccgc ggaaatgata tcggaatgat
    28141 tttccaagat ccgttgtctg cattaaatcc tctcatgaga atcgaagacc agatcaaaga
    28201 aggactttcc tatcacacaa agatgaatgc agaacagcgt caagctcgtg cactagaatt
    28261 gctagaacaa gtagggattc ctaatcctgc gagagttgga cgccaatatc cccatgaact
    28321 atcaggcggt atgagacaac gtgtcatcat cgcaatcgca atcgcctgca aacctccaat
    28381 cgtcattgca gatgaaccga cgacagccct agatgtaacc atccaagcac agattcttga
    28441 tttactgaaa gaccttcagg aagaaacaaa aacagggatc atcttgatca ctcatgactt
    28501 aggagtcgta gcagaaatgg ctgatcgagt ggcagtgatg tatggcggac agttcgttga
    28561 agtagcaggt gtaaaagaac tgtttgatca tcctaagcat ccttatacac gctctttact
    28621 gaactctata ccacaagaag gaaatcatga tgcagaactc catgtgatcg aaggtgtcgt
    28681 tccttctctg aaaaacatgc ctcgtaaagg ttgtcggttt gcaccacgta ttccatggat
    28741 accagcaagc gatcatgaag aagagcctat ccttcatgaa gtcgcaccga accacttggt
    28801 acgttgctcc tgctataagc atttccactt tagagacgaa aaaggagagg tgtagatgac
    28861 agaattaatc acaatcaaag acctgaaagt tcattaccct attcgcagtg gattcttcaa
    28921 tcgagtaacg gatcatgtat atgctgtaga tggtgtggat tttattatcg aaaaaggaaa
    28981 gacatatggt ttagtgggag agtctggctc tggaaaatct actacgggaa aagccgttgt
    29041 gggattagaa aaggtaacag ccggacaaat catgtatgaa ggaaaagatg taacgaagcg
    29101 aagcaaccgc aaaaaaatgg gttataacaa ggatgtgcag atgatcttcc aagattcaat
    29161 gtccagtttg aatcctaaaa agcgagtgtt ggatatcatt gctgaaccca tccgaaattt
    29221 tgaacggtta agcgatcaag aagaaaagaa aaaggtaaaa ggactgctag atatcgttgg
    29281 gatgccggaa gatgcgctat ataaatatcc tcatgaattt tcaggcggac aacgtcaacg
    29341 tctaggagta gcccgtgctg ttgctaccaa tccaaaactg atcgtagccg atgaacctgt
    29401 atctgctctg gatctatctg ttcaagcgca agtattgaat ttcatgaaac gaatccagca
    29461 agaatacggc ctcagctatt tattcatttc acatgattta ggtgtagtaa aacacatgtg
    29521 cgacaatatc gcaattatgt acaaaggtcg atttgtagaa atcggtacaa gagaagacat
    29581 ttataatgac ccgcgacata tctatacgaa acgtctgctt tcagctattc cgcaaatcga
    29641 tgtagaaaat agggaaagcc acaaagaaaa tcgacgaaga gtagaacaag agtatatcca
    29701 aaaccaaaaa gaatactatg atgcgactgg tcgagtctac gatttgcgta cgatcacaac
    29761 tacacacaaa gtggcgttga aagatggagg tgcaagctaa tgtggaaaac gattctgcga
    29821 cgtgttttat tgatgatccc gcaagttatt attttaagtg ttctgatctt cttattagcc
    29881 caagcgatgc cgggggatcc atttaccggt ttgatcaatc caaatcagga tccggcagtg
    29941 atcgaacaga tgcgacaagc agctggcttg aatgacccat ggtatgtcca gtacttccgt
    30001 tggatcggaa atgctttgca aggcgatttt ggtcaaagtt tcttatacaa acgctctgtt
    30061 gctgacttga tcggcgaacg gatcatcaac actttatatc tatcgatctt gacagttgtc
    30121 cttacttatt tgattgctgt gcctctgggg atgcttgctg gccggtatca aaattcaatc
    30181 ttggataaga ttgtcgtaat ctacaacttc tttagttttg ctgttccttt atttatcttc
    30241 ggcttgatca tgctatttgt ttttggttat cgtttaggct ggttcccaac aagtggatca
    30301 caaacactag gtttgcaagg tggatttatc actcaatgga tagaccgttt gaaatatatt
    30361 ctgttaccat caattaccca agcatttttg gcgactgccg tcacaatcca atatctgcga
    30421 agtgaagtaa tcgattcgaa atcgctcgat tttgtcagaa cagcccgttc caaaggggta
    30481 ccgacgaata aagtattcag tcgacatatt ttccgaaatg cagcgttgcc gatggcttct
    30541 caaatggggt atgaaatcac ttcgttgatt gctggttctg tagttatcga aaagattttt
    30601 ggttatccgg gagtcggaaa actatttatt gattccatcg gccaacggga ctataccgtg
    30661 atcacatcct tagtattgat tttaggaatc gcgacgctag tggggacatt actttcagat
    30721 atcatcatga gtatcgttga cccgcgtatt cggattcaat aaaaccaatc agaaaggaga
    30781 atggatcgat gagtgaaaaa aacaatacag cagttcaaga aagtattcct cctatgggga
    30841 tacgaatgat tgctcgagaa tttaaaaaag ataaaattgc tattttttca ttaggtttgc
    30901 tagtggtttt attattagtg atctttatcg gtgcgatgct gaccgatcaa gacaaagtga
    30961 tgtatgttag tatcttcgac aaatacgctg caccaggaac agtttcgaca caaggacaaa
    31021 agttcattct gggagcagat gaaggcggac gtgatgtatt aggacaattg atcataggtg
    31081 ctagaaactc tgttacgatc ggatttgcga tcacgatcct tacttcgatc atcggggttg
    31141 gattagggat catctctggc tactacggag ggatcattga caacatcttg atgcggatcg
    31201 ttgattttat catgattcta ccgatcatgc tgatcattat cgtcttcgta tcgattattt
    31261 ccagatacaa tgtctggtcc tttatattca tcatgagtgc attttattgg gtagctaaag
    31321 cccgattatt ccgaagcaag acattatctg aagcaagaag agattatgtc agtgcctcaa
    31381 aaacaatggg aaccaatgat tttaaaatca tgttccgcga aatcatgccg aatctaagtt
    31441 cattaatcat tactaatttg acgatgaact ttgcggcaaa catcggtatc gagacaactt
    31501 tgactttctt aggtttcggc ttgccggcaa atgtacctag tttaggtaca ttgatcggtt
    31561 atgcaagtaa cggagaagtc ttagtgaatc ggcaatggat ctggttaccc gcatcaatcc
    31621 ttattttagt tttgatgttg agtatcaatt atgttggaca agcattcaag cgttctgcag
    31681 atgcgcgcca acggttaggc tagtatagaa gcgaaggggg aaataggaat gaagaaaacg
    31741 atttttggat ttgcagcgct gatgtctgct gtcgttcttg cagcatgcgg gggcggaggt
    31801 acagatacct ctggaaacaa ctctggaggc aacggcaata acaaagagga attaactttt
    31861 ccaattgcaa caaaaaataa tgatgatgca atcgaagacg gcgaattaga agctgcggtc
    31921 gcaacggaca gtcagtttaa aggattgttc cagtgggagt tttaccaaga ttcatatgat
    31981 gcagctttta tggctccttc tcatgaaccg ctatttgcaa acgatgacga tttcagaatc
    32041 acagacgacg gagctgcaac gcttgatcta gatgaagata cgaaaaaagc gacaatcaaa
    32101 gtaaaagaca acgtcaaatg gtcagatggt gaagatgtaa cggcggacga tgtgattttc
    32161 ccttacgaaa tcattgggaa taaagattac actggtatcc gttatgacga cactttccgt
    32221 aacatcgtag gtatggaaga atatcatgat ggaagtgccg acacaatctc cggaatcaaa
    32281 aaaatagacg acaaaacagt tgaaatcgaa tataaagaag taaacccaag catgcttcaa
    32341 tcgggtggtg gtatctggtc atatgcagca ccaaaacata cgttagaagg tgtagcaatc
    32401 aaagatatgg aatcaagcga tcaagtacgt aaaaatccag tgacttttgg cccttattac
    32461 atgagtaata tcgttgcagg agaatcagta gaatacttgc caaacgagca ctactggaat
    32521 ggtacgccta aattgaaaaa gatcacgatg aaatctgtac cgatggcaag tgcaactgaa
    32581 gcattgaact ctaaattata tgatatgatt ttccaaatgc caacagatac ttacgatacc
    32641 tataaagaaa ttgctggcta tacaaactta ggccgtgaac aaacttctta tacttatcta
    32701 ggattcaagt taggtaaatg gaatgcagat acaaagagtg tggaatacaa tccagattca
    32761 aaaatggctg ataaatcatt acgtcaagca atgggctatg cattagataa taatgcagta
    32821 ggtgaacgtt tctatgcagg tttgagaagc aatgcgactt cattgattcc tccagtattc
    32881 cagtcatacc atgatgatga gatgaaagga ttcacacaag atctagacaa agcgaaaaaa
    32941 ctattagatg atgctggcta caaagatgtg gatgacgatg gtttaagaga agacaaagat
    33001 ggaaataaac taacaatcaa ctttgcttcg atgtccggtg gggaaacagc gcaaccattg
    33061 gcagattact atatccaaca atggaaagaa attggtttgg atgtccaata cacaacaggt
    33121 cgtctgatcg aattcaattc tttctatgat aaattagaaa acgatgatcc tgaaattgat
    33181 gtttaccaag ctgcttggaa tacaggaact gatccaaatc caagtggact atggggacca
    33241 aatgctgcgt tcaactacac tcgttatgaa tcagaagaaa atacaaaatt gatcaacgat
    33301 atcagttcaa gcaaagcatt tgatgaagag taccaaaaag aagcatatca aaaatggcaa
    33361 caatatgcat ttgaagaagc atttgcgatt ccaacgttgt tccgtaatga agtattgcca
    33421 gtcaacgacc gtgtcaaaga ctgggattgg tcttatgatg taccaaaccc atgggcaaat
    33481 gtttctgtta cagcagattc acgttcataa tcactaaaaa tgaccgaaaa agggcgatgt
    33541 cctttttcgg ttttttgata tttttctgtt tatttctcta atattcaggt caaggcgctg
    33601 atttcatgac ttctccaata aacggtgttc aaagtccagt ttctcggtat taagtcaaat
    33661 ttatgcaaaa atatggaaag ccattttcac aaaatttttt cttcatactc gaaactaaac
    33721 gaatttttca caacttctcg tcaatcacta ggattattaa agctttactt accttttgac
    33781 cgaaccattt caaatttgac aaaaaaccag tataataagg aagtatcgaa aaaatatcca
    33841 taaaaggaat gtggaaaatg gacaaccagc tgacaaaaga actaaaagaa aactataaca
    33901 tcgtttttca cgatctaaat ttgcttgaac aagcttttac ccattcatcc tatgtgaatg
    33961 agcatcgcaa tctgcaatta tcggataatg agcgacttga atttttagga gatgccgttt
    34021 tagaactgat ggtttctcag tatttattcc gtttatatcc tcatttgcca gaaggaaaat
    34081 tgacaaaaac acgagctgcg atcgttcgcg aagacagctt gtcgaaattt gccaaagaat
    34141 gtcactttga tcaatatgtt ttgttgggca agggagaaga gaattcagga ggacgtacgc
    34201 gaccagcatt gctttgcgac ctattcgaag cttttttagg cgcgttatat cttgaccagg
    34261 gattcgacac gacccatgca ttcatcgaaa aagtgatttt tcctaaagtc aaagcaggtg
    34321 ctttttcaca tgagatggat cataaaacaa aactacaaga agtcttgcaa aaatcaggtg
    34381 atgtatcaat cgaataccga ttgatcaatg aagaaggtcc agctcatgaa cgagtatttt
    34441 ggatcgaagt ttatgtagat gatcaattga ttggcactgg acaagggaaa tcgaaaaaat
    34501 tagcagaaca agctgcggcg gaaaatgcat tggcagcact gtaaaagtaa gtgagggaaa
    34561 gctttgtatt tgaaacgaat tgaaatagcc ggttttaaat catttgcaga caagacagtc
    34621 attgattttg aaaatagcgt cacagcagtg gttggaccaa atggcagcgg aaaaagcaat
    34681 attacagagg ctatccgctg ggtattagga gaacaatctg ctaaaagtct gcgaggcgga
    34741 aaaatgccgg atatcatctt tgccggttcc gatacccgta agcagctgaa tattgcagaa
    34801 gtcacagtga ttttagacaa tagtgatcat tatctgccat tggagtacaa tgagatcagt
    34861 gtaacccgcc gttatcgacg gactggggaa agcgagtttt tcatcaataa acaaagctgc
    34921 agattaaaag atatccaaga attgttctta gattctggat taggaaaaga atcgttctca
    34981 atcatttctc aaggaaaagt agaagcgatt ttcagtagta aaccggaaga ccgtcgtggg
    35041 atctttgaag aagcagctgg tgtcttgaaa tacaagcagc ggaaaaagaa agcagaacag
    35101 aagctttttg aaacagaaga caacctcagc cgcgtccaag acatcatcca cgaattggaa
    35161 gaacagttaa ctcctttggc cgcccagagt gaggcagcaa aggagttttt acggctaaaa
    35221 gaaacactga ctcaaaccga tgtctctttg atggttgctg aaatcaagac agcaaaaaaa
    35281 gattgggaca acaaacaagc acagcttgcg aaattcaatc tagaattagg caaactatct
    35341 gagtcgatcc aagaacaaga atccattttg gcgaagcagc gtaaagaaaa tgcgcaagcc
    35401 gatcgtctga tcgaaaaaaa tcagcaagta ttattagatc tttcggaaaa actgaagcag
    35461 acagaaggac aaaaagatgt tttgcaggag cggacaaagc atacacaaaa aagctcgcaa
    35521 gaatatcaaa cttcattagc agaagcacaa aaaaaggtta aacattttga aaagcttcaa
    35581 gaaagcttga tgaaagcagc agcagaaaaa gaaacagaga tccaaaaagc tgaagcaaat
    35641 ctcataaaaa cacagcaaga attagaaaaa taccaaaaat caacgaaaga attattggca
    35701 gaattaagag atcaatatgt tgatttgatg caagaacaag cagctgtagg aaacgaactg
    35761 aagtatctag aacgtcaata tatacaagaa acagcaaaaa gcaagcagac gttagccaag
    35821 caatcggaag tagaagcatc agtagaccga ttgatcttac aaaaagaaga attgacacaa
    35881 aagcaggcgc agctaaaatc atctttaact gaaacaaaag agaagttaga aatgatccaa
    35941 caaaacggga aaaagttcca agaagcatta gcaaaagaac agcctaaaat gtatcaatta
    36001 atgaatcaag tccagcagct acgtgctcgt aagaaaagcc ttcaagaaat ccaagaaaac
    36061 tattttggtt tttaccaagg ggttcgattg gtcttgcagc ataaacagca gctttcgggt
    36121 attgtcggag cagtggctga actgatcgat gttcctgcag atttcacgtt agctatcgaa
    36181 gcagctttag gtggggcagc ccagcatgtc atcgttgaaa atgaaaaaga tgctcggcaa
    36241 gccatcacgt atttgaaaca gcaaagagga ggaagagcaa cattccttcc actgacgaca
    36301 atcaaaccaa gacaattacc ggctcatata ttgacacaag cagcagctgt agaagggttt
    36361 atagggattg ccagtgaaca agtcagttat cccgatcaaa tccaaactgt ggttcaaaac
    36421 ctgctaggaa cgatcctttt agccaaagac ttaactagtg ccaatgcaat cgcgcaaacg
    36481 atccgctatc aatatcgagt ggtttcctta gaaggtgatg tgatgaatgc cggaggatcc
    36541 atgacaggtg gagcgaataa acgaggaaac caaggcagtt tgtttgtaca aaatcaagaa
    36601 ttgaaacagt tgactagtga atttgaagaa gcagataaac aactccaagc acaagaaaaa
    36661 aaagttcagg agctgcaaca agaaacagct cgtttagccg aagaacaaga agttttgcgt
    36721 acacgaggcg aacaattacg ttttgaagaa caagaagcga caaatcagct acaaaacatc
    36781 ataaatgaac ttgaacgttt tgaaaaagaa aagcaaatct caacttttga gacacgtgaa
    36841 ctgcagcagt ttatcgaaga ttacgaaaag caaacgaacg aattgaaaga caaacaaaca
    36901 gatcttgaat cgcaacgaca aaaaatcgac gaagaaatca aatcgttaag tcaagaaagc
    36961 gaccagatgg aagccagaag agcacaagta caaagtcaaa aagcacaaga acaagcagat
    37021 cttgctgtct tgaaagagca gtttaatcat ctgcagatcc agctccgcgg tgcccgagtc
    37081 caaaaagcag aggcaaccga acgtcaagaa gcgatagaaa agcagttggc gacattgaca
    37141 gcagactttt ctgatcatga agtcacagaa gaaagtttgg aaaaacaaat taacgaatta
    37201 tctgctcagc gagaaacatt aaaagccgag ttggcaaaag ccaaagagca gcgagataga
    37261 aggcaaaagg agatcgataa gcttgaagca gtcttggcag agcgtaatcg tgagcaaaaa
    37321 gcacgactgt cggaacaatc caaattagaa gtccaaaaag accgagctga aatgatttta
    37381 gataatcatt tgacctatct gcagtcagaa tatcagctga catttgagaa agcttcccaa
    37441 gactatcaag aaacaaccga tatcgaagac agtagaacaa aggtttcatc cttaaaagaa
    37501 cagattgaaa aactgggacc agtaaactta aattcaatcg aacaatacga acaagtcagt
    37561 gaaagacata cattcttagc aacacagcga gatgatctgt tagcagcgaa aaaccaattg
    37621 tttgaaacga tggatgagat ggatgatgaa gtgcggacaa gattcaaaga agtatttgaa
    37681 gctatccgtc aagaattcaa aattgtcttt ccaaatatgt ttggcggcgg taaagctgaa
    37741 ttagtactga cggaaccaac tgatttgctg aaaacaggta ttgagattga agtacaacct
    37801 cctggcaaaa aactgcaaag tctaagtttg ctttctggag gagaacgagc acttacagcc
    37861 atcgcgttgc tcttttccat tatccgtgtc tgtcctgttc cgttttgtat cttagatgag
    37921 gtagaagcag cgttagatga agccaacgtg aaacgttttg gtcgttactt gagcgatttc
    37981 caagatgaca cacaatttat cgtagtcaca caccgcaaag gaacgatggt cgcagcagat
    38041 gtactgtacg gagtgacgat gcaggaatcc ggtgtatcaa aaatcgtttc tgtacggatg
    38101 gaagatatca atgaagaggc gaaaatcaac gaaacgaaag tgtaggaacg aaaatgatca
    38161 aattagtagc aatcgatttg gacggtacat tattaaatga acaaaagctg atttcagatg
    38221 agaataaaca agcattagca caagcaaaac agcaaggggt caagatcgtt ctatgtacag
    38281 gacgcccctt agctgcgatg gcgcattatc tgcaagaatt aggactggta gatgaaggtg
    38341 actacagcat cactttcaac ggcggtttgg tacaaaaaaa tgatactggt gaaatcatgg
    38401 aaaagaaagt gatggaagta tcagatatcc aacgcttata caaactggct cagcaattgg
    38461 atctgccact agatgttttg tctgacaatg tggttttaca gctgcctagt gccccaagga
    38521 agcaatcctt gtacaatgtg ttgaataagc tgctgcaatt ccaaccagcg gatataagcg
    38581 atattacaga tgaatttgtg cttaataagg cagtaatcgc ttatgaccaa gaagaattag
    38641 atcctaaaat caaagaaata ccagcagaat tccatgaaat gtatgagatc atcaaaacaa
    38701 gaagtatgct tttggaattt atgccaaaag gtgttacaaa agcttatggt atttctttat
    38761 tggcaaaaga tcttggtttg aaacaagaag aaatcatggc gatcggtgat gaggaaaatg
    38821 atttaccgat gatccaatat gctggactag gcgtagcgat ggagaatgct gtgcctttcg
    38881 tcaaagaagc agcagatttc gtgacagcca gcaatgaaga aaatggcgta gccaaagtga
    38941 tccaaaaatt tgtattagag tagggggagt caaatgggat tttttgataa aatcaagcgt
    39001 gcttttactg gcgaagagaa aacagaagaa gttgaagtac aggagaagta cgacaagggt
    39061 ttagaaaaga cgcgtaaaac atttggtgaa cggatgaatg agttgtttgc caattttcga
    39121 acagtcgatg aagacttttt tgaagaatta gaagaaacac ttatcggtgc agatgtaggt
    39181 tttgaaacag cgatacgaat caccgaagca ttacgtcaag aagtgaaact cagaaatgcg
    39241 aaaaaatcgg cagaagtcca aaatgcgatc atcgagaaac ttgtggatct ctatgaagcg
    39301 gaaggaatca atgaagtaaa tgaactaaat atccaagaag acggactaac agttattttg
    39361 tttgtcggtg ttaatggtgt agggaaaaca acaagtattg gtaaacttgc ccatcagttc
    39421 aaaaacgaag gtaaaaaagt attgatggca gcagcagata ccttccgcgc tggagcaatc
    39481 gaccagttag ttgtctgggg cgaacgcgct ggtgttgaag ttgttcgtgg caatgcagga
    39541 ggcgatccag cagctgtcgt attcgatgct ttggaaagag caaagacaga gcaggcagat
    39601 gttttactag ttgatactgc aggccgactg caaaataaag tgaacttgat gaacgaatta
    39661 gaaaaaataa aacgagtaat ccagcgggaa ttaccagaag caccccaaga aacacttctt
    39721 gtattagatg caaccactgg tcaaaacgcg atggttcaag caaaacaatt caaagaaaca
    39781 acagatgtga ccggtcttgt tttgacaaaa ttagatggta cagcaaaggg cggtatcgta
    39841 cttgctatcc gtaatgaatt gcatttacca gtgaaattgg tcggtcttgg tgaaggaatc
    39901 gatgacttgg agccgtttga tccaaatgat tttgtcgttg gattgttcaa aggattacta
    39961 aaagaagaat aacccatgtc taaaaagcag gagctgtgac aaaagttgca gctccttgtt
    40021 tgaatgattg tttttaacat agctgtgttt ttgatgcctt catttttata cttatttgta
    40081 agcggttctc aaacaatttc gttctttttc ttagactttt tgattataat tagattcata
    40141 tgaattggag gtcacttaat gatgaatttt aagcaattat tattgcatgt caatgcgcgc
    40201 ccttttatcg accaagctcg atttggtata gaacgggaag gacagcgggt tgatcttgca
    40261 ggaaatctag caaaaaccga tcatccagca atctttggcg atcgatccta tcacccctat
    40321 atccaaacag attttagcga aacacaaaca gagatgatca cccctgttac cgattctatt
    40381 cccgaattat ttcagtatct cgctgctgtt tatgatgtga ctgctcgttc tataccgaaa
    40441 gaagagatga tctggccatt aagtatgcca cctgccttac cggaaaaaga cgaagagatc
    40501 attattgcaa aattaaaaaa tttcgaagat gtcttgtatc gacgttattt agcaaaagaa
    40561 tacgggaagc gaaaacagat ggtaagcggt attcatttca attttgaatt tggcgatgag
    40621 ttgctaagaa cattgttcag ccatcaggaa gaatttcaag atttttccga attcaaaaca
    40681 gaactttatt tgaaaacagc gagaaacttt atgcgctatc gctggatgat cacttattta
    40741 ttcggtgctt caccaatgag tgagaagaac tactttttag atgaatcaca tccgcaagaa
    40801 cctgttcgca gtattcgcaa cagtgcgctg ggctatacga atcatccaaa tgtgaaagtt
    40861 tcctatgctt ctatgaaaca gtatttagca gatatcgagc gaatgatcga agaagggaaa
    40921 ctttcggagg agaaagaatt ttatacaccg cttcgtttcc gaggcggaaa gaaagtcgca
    40981 gatttagcaa caacaggtgt tcgttatatt gaattgcgga atatcgattt gaatccttat
    41041 gcaagattgg gtatcaatcc agagcaagtt cggttcttac aattattcct gatgtacatg
    41101 ttatggacag aagaaaaaga agactgtgac caatgggtag cagaaggtac gactcgaaat
    41161 aacaaagtag cactagaaca accatcagat caaacagaat ttcatcaaga aggtagagaa
    41221 attcttgaag gaatgaagca aatgctggtt gaattggatt ggttggattc tctttattta
    41281 gtagaggaag cattgactca aatggatcat cctgaacaaa cgttagcagc gaaactctat
    41341 caagaagcac agctatcgag tcagcaagaa gttgctgtgg cattgggaca tcaatattat
    41401 aaagaaagcc atgaacgtcc ctatcagttg gccggttttc gcgaaatgga actttctact
    41461 cagatattta tgttcgatgc catccaaaaa ggtgtccaag tcaaagtatt agatgagtcg
    41521 gatcaatttt tgcgtctgca attccaagac catgtagaat atgtgaaaaa tgcaaacatg
    41581 acaagtaagg acagctatat cgtcccactt atcatggaaa acaaaacagt aacgaaaaaa
    41641 gtcttaaaag aagctgggtt ccgggtacca ggaggcgcag aattttcatc catggaagaa
    41701 gcggtaaaag cttatccgag atttgccgag caggcatttg tgatcaaacc taaatcaacc
    41761 aattacgggt taggtatcac gattttcaag gaaggagcta gtctggaaga ttatcaagca
    41821 ggtttagcca ttgcttttcg tgaagacagt tcggttttag tagaagaatt tatgccggga
    41881 acggaatatc gtttctttgt gatcgacgga gaagtccaag ccatcatgtt gcgagtccct
    41941 gctaatgtta ttggtgacag tatccgaaca gtgaaagaac ttgttgaaga aaaaaatagt
    42001 gaccctttgc gtggaacgaa tcatcgtgca cctttagaat tgatccaatt aggtgaatta
    42061 gaacaattga tgttgaaaga acaagggttg acgatcgaat cagttcccca agccaatcaa
    42121 attgtttatc taagggagaa ctcaaatatc agtacaggtg gtgattcgat cgatatgact
    42181 gatgaatttt ctgaagctta caaaaaaatc gccgtttctg cggtagaagc actaggagct
    42241 aagattagtg gtatcgattt gattatacca gataaagaaa tcgatcctac aactgataaa
    42301 aaagcatatg ggatcatcga agcaaatttc aatccagcca tgcatatgca tgtttatcct
    42361 tttgcaggaa aaggcagaag attgacgatg aatgtattga aattgttata tcctgaagta
    42421 ttttaaaaga aaaagctttt gacagattgt cctatttgat gtcaatctga tcaaaagctt
    42481 tttttgcatg taaaaattta tgtgtttttt cgttttaaaa taaacgatcc cagaagcttt
    42541 ttttcttttt tggtttttct ttttcagctg gcgaagagtt ttcttctggg tatttttttt
    42601 cgatatcaat cacaggttca tttactgctt cttttgctgt taagagcaat ccgaattcct
    42661 caggatttgc agtgacgtaa tcgttgacta cagtgaaacg gctattgttt ttcgttgcta
    42721 gctggatata ttggttttgg acagaaaggg gtagagcccc attcaagagt atggaagcat
    42781 ctggatgttt tttgagttct tttaaaaagt tttctttgtt ttttggttgg atcatttcag
    42841 caacagtcat actaagatag cagcgttccc tgaatgtccc taaatattta tgctgttctt
    42901 cagggttcac taaaggtgta ccataaagtc ccttatccaa atggcttttt acatcatctt
    42961 gtgccataaa aaataccctc cttcttctct ttgtctttct tacgattata gcatattccc
    43021 aataatacct aaaacatatg aaaaagcggt tgcttttgtt caacgattca cttaaaataa
    43081 gaatgaaaat cattctcaat tagaaaggtg gattggatgc gctacggacg actagtagca
    43141 ttattagtta ttttaagtag tatttcattg ttcatcggag tatcgaatgt acaattgtca
    43201 gaactgctcc agttagatgg gcaaagctta caaatcttgc tagtgactcg tttgccacga
    43261 acgatttgtt tgatccttgt gggagcgacc agtagtattt gtgggttgat catgcaacat
    43321 ttgacccaaa ataaatttgt ttcaccaacg actgcgggca cgatggatag tgcgcgttta
    43381 ggtattttag tggctatgat tttcctacct ggtgcatcct tgattgtccg atccttgaca
    43441 gcattttgct ttgcttttgc aggaacattg ctttttttat cccttacgcg attgttcctc
    43501 cagaaaaatc aattgatgct tccgttgatt ggagtcatgt tagggaatat cattggttca
    43561 gttgctactt tttttgctta tcaattccag ttggtacaaa atatgacttc atggcttcaa
    43621 gggaattttg cgactgtgat acgaggaaat tatgagttat tgtatgctgt tgttccattg
    43681 ttgctcttgg tttggctatt tgcttaccga tttacgatca ttggtctggg cgaatcgttt
    43741 gcagtaaatc tgggaataga ttaccagaag atgcagtttt taggcattgg cttagtatca
    43801 ttggctagtg cagtcatgtt gatcatggta ggaagcattc cttttatcgg agtgatcatt
    43861 ccgaatcttg tctctagaat atatggagat caagtgcaca aaacaattgg cgtaactgct
    43921 ttagcaggta gtttgttctt agtattttgc gatcttgtcg ctcgcgtgtt gatttttcct
    43981 tatgaaatac cagtcagtgt gatcgttgga atcatcggtg gggctatctt cctttatctt
    44041 ctgataaggg gacgacgata tgcttaaaaa
//
LOCUS       DACDIH010000019        41192 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDIH010000019 DACDIH010000000
VERSION     DACDIH010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.019"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..202
                     /locus_tag="IV281_001057"
     CDS             <1..202
                     /locus_tag="IV281_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8780088.1"
                     /translation="ILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            217..885
                     /locus_tag="IV281_001058"
     CDS             217..885
                     /locus_tag="IV281_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8780089.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            905..1483
                     /locus_tag="IV281_001059"
     CDS             905..1483
                     /locus_tag="IV281_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8780090.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            1886..2797
                     /locus_tag="IV281_001060"
     CDS             1886..2797
                     /locus_tag="IV281_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8780091.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(3053..3970)
                     /locus_tag="IV281_001061"
     CDS             complement(3053..3970)
                     /locus_tag="IV281_001061"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP8780092.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            4172..4609
                     /gene="zapA"
                     /locus_tag="IV281_001062"
     CDS             4172..4609
                     /gene="zapA"
                     /locus_tag="IV281_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP8780093.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            4667..5215
                     /locus_tag="IV281_001063"
     CDS             4667..5215
                     /locus_tag="IV281_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP8780094.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            5330..7690
                     /locus_tag="IV281_001064"
     CDS             5330..7690
                     /locus_tag="IV281_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP8780095.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            7777..8091
                     /gene="trxA"
                     /locus_tag="IV281_001065"
     CDS             7777..8091
                     /gene="trxA"
                     /locus_tag="IV281_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP8780096.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            8273..10051
                     /gene="uvrC"
                     /locus_tag="IV281_001066"
     CDS             8273..10051
                     /gene="uvrC"
                     /locus_tag="IV281_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP8780097.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            10196..11095
                     /locus_tag="IV281_001067"
     CDS             10196..11095
                     /locus_tag="IV281_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8780098.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            11088..12350
                     /locus_tag="IV281_001068"
     CDS             11088..12350
                     /locus_tag="IV281_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8780099.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            12513..14411
                     /locus_tag="IV281_001069"
     CDS             12513..14411
                     /locus_tag="IV281_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP8780100.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(14710..15429)
                     /locus_tag="IV281_001070"
     CDS             complement(14710..15429)
                     /locus_tag="IV281_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP8780101.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            15766..16098
                     /locus_tag="IV281_001071"
     CDS             15766..16098
                     /locus_tag="IV281_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780102.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            16085..18088
                     /locus_tag="IV281_001072"
     CDS             16085..18088
                     /locus_tag="IV281_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP8780103.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            18107..18577
                     /locus_tag="IV281_001073"
     CDS             18107..18577
                     /locus_tag="IV281_001073"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP8780104.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            18598..19182
                     /locus_tag="IV281_001074"
     CDS             18598..19182
                     /locus_tag="IV281_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP8780105.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            19197..20183
                     /locus_tag="IV281_001075"
     CDS             19197..20183
                     /locus_tag="IV281_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP8780106.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            20173..20484
                     /locus_tag="IV281_001076"
     CDS             20173..20484
                     /locus_tag="IV281_001076"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP8780107.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            20506..22287
                     /locus_tag="IV281_001077"
     CDS             20506..22287
                     /locus_tag="IV281_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP8780108.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            22280..23656
                     /locus_tag="IV281_001078"
     CDS             22280..23656
                     /locus_tag="IV281_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP8780109.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            23663..24298
                     /locus_tag="IV281_001079"
     CDS             23663..24298
                     /locus_tag="IV281_001079"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP8780110.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            24328..25683
                     /locus_tag="IV281_001080"
     CDS             24328..25683
                     /locus_tag="IV281_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP8780111.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(25875..27269)
                     /locus_tag="IV281_001081"
     CDS             complement(25875..27269)
                     /locus_tag="IV281_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8780112.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            27361..28332
                     /locus_tag="IV281_001082"
     CDS             27361..28332
                     /locus_tag="IV281_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP8780113.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            28329..29111
                     /locus_tag="IV281_001083"
     CDS             28329..29111
                     /locus_tag="IV281_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP8780114.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(29280..29651)
                     /locus_tag="IV281_001084"
     CDS             complement(29280..29651)
                     /locus_tag="IV281_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8780115.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            29785..30228
                     /locus_tag="IV281_001085"
     CDS             29785..30228
                     /locus_tag="IV281_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8780116.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(30271..31080)
                     /gene="yidA"
                     /locus_tag="IV281_001086"
     CDS             complement(30271..31080)
                     /gene="yidA"
                     /locus_tag="IV281_001086"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP8780117.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(31099..32466)
                     /locus_tag="IV281_001087"
     CDS             complement(31099..32466)
                     /locus_tag="IV281_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8780118.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(32450..33280)
                     /locus_tag="IV281_001088"
     CDS             complement(32450..33280)
                     /locus_tag="IV281_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP8780119.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            33443..33880
                     /locus_tag="IV281_001089"
     CDS             33443..33880
                     /locus_tag="IV281_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP8780120.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            33956..34576
                     /locus_tag="IV281_001090"
     CDS             33956..34576
                     /locus_tag="IV281_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP8780121.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            34765..35271
                     /locus_tag="IV281_001091"
     CDS             34765..35271
                     /locus_tag="IV281_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP8780122.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            35288..36187
                     /locus_tag="IV281_001092"
     CDS             35288..36187
                     /locus_tag="IV281_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP8780123.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            36274..37158
                     /locus_tag="IV281_001093"
     CDS             36274..37158
                     /locus_tag="IV281_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8780124.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(37350..37421)
                     /locus_tag="IV281_001094"
     tRNA            complement(37350..37421)
                     /locus_tag="IV281_001094"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37386..37388),aa:Arg,seq:ccg)
     gene            37481..38815
                     /gene="rpoN"
                     /locus_tag="IV281_001095"
     CDS             37481..38815
                     /gene="rpoN"
                     /locus_tag="IV281_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP8780125.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            39018..40055
                     /locus_tag="IV281_001096"
     CDS             39018..40055
                     /locus_tag="IV281_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP8780126.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            40096..41097
                     /gene="gap"
                     /locus_tag="IV281_001097"
     CDS             40096..41097
                     /gene="gap"
                     /locus_tag="IV281_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8780127.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
BASE COUNT        13609 a         7113 c         8787 g        11683 t
ORIGIN      
        1 cattttatca tttgttggta ttcttcttgg tctattagga acagtgggaa catcccttgt
       61 tctacctgat gcggttccat tccaaagcaa ttggctgttt tttggcgtca ttagcttgtt
      121 gatgctggta gtagctgtgt taggcgcact attttctgtt cggacaatcg taaaaatcga
      181 tccgttgaag gcaattggat aagaaggagg aagagcatga aagcaataga atttaaagaa
      241 gtagaaaaaa gtttccaaga tggcgaccaa gtagttaaag cattgaaaga aacaaccatg
      301 agtatcgata aaggggaatt tgttgcaatc atcggtcctt ctggttcagg taaaagtact
      361 tttttgactt tggcaggcgg attgcagacg cctagcagtg gctctgtatt tatcaatgat
      421 caagaattca gtaatcaaaa agaaagtgaa cgcgttaaac tacgttttaa agaaattggc
      481 tttattttgc aagcttccaa tctggttcct tttctgacag taaaaaagca gttattatta
      541 gtagataaga tcaaacatga acatcgtcaa aaagaagcaa aagaattatt tgagcagctt
      601 ggcgtggata agttgatcaa caagtatcca gaagaactat ctggtggaga acggcaaagg
      661 gtcgcaatcg ctcgtgcatt gtacaatgat ccgtccatta ttctagcaga tgaaccaact
      721 gctagtttgg attcagaaaa ggcacgagaa gtagtcgata tcttggcaaa agaatcaaaa
      781 gacaaaaata aagcaacgat catggttact cacgatacac ggctgattga caaatgcgat
      841 agaatattta tgatggaaga tggtgtattg aaagaaaaag aataaccaaa aagcaggtgg
      901 aaaaatggtc catcgataca acaaagatac acagcaaaaa attttgaaag aagcgactga
      961 cttgtttatg tccaagggat atctcggcac ttcaacaaga gagatcgcac aaaaagttgg
     1021 gatcacccaa cccaatctct atcattattt cggtgataaa gaaaaactat atacggcagt
     1081 tttagaaaac cacttaaaag atgttggaaa agctttgaga gaaatcgttc aaacaagcga
     1141 aacgggattt caagtaacct tgacaaaaat ggcgcagttt ttgattgaga cacatctggt
     1201 cgatttgttt ctgatgcttc acgatcttga aagcaatctt tcaaaggaaa caagagatca
     1261 tcttttcttt ctatggaaga aaaattatcg tgaacctttt gaagaaatct tttcagcaaa
     1321 ccaatctgtg ctaagaaatg ggatttcaaa agaaatcgca gcaaggcatt ttttcttagt
     1381 gttagcacct tacatcactc ggtcgaaaag acctgtggaa gaaccgctaa atgtggcgca
     1441 attgatcgat ttatatttga atggtgtatt aggagaagcg taaagctgtt atatagggag
     1501 aaaaatagtt aggtgaagaa gcaagatatg acgaagatcg ttatatcttg ttttttttat
     1561 ataggtagtt tttcataaat acaaatttgt tattctctca tgattatcag ggtatatttt
     1621 tatttattat gtcggtgttc caggaagtgt tttaatttaa taacgaagaa attaattgtt
     1681 tgtagaatga aggaaactat ttattgggta gatgaatcac cacgaatagt caaaaggaga
     1741 tttcaggata tgccaaaaat gaaaaaaatc attttagcag taatgttatt ttctacggtt
     1801 atatctttta cagccccttt tttgcgtact taagctgatt ttaagttttg acccttagtc
     1861 tggatctacg aggtgagagt caagaatgaa aaaaataatt ggagcttttg tatttgcaat
     1921 cattatcatt attatctgtt ttcaaataga tgtttttggt caaaaaaaga tagttggaca
     1981 aacggtcagc agtacgaaca gtacggttac aaagacaaag gaatcatttg atgatacaga
     2041 acaagagaca ttaatcaaaa tcggaaaaaa tgaccgtaca ctatatacag atgactcgct
     2101 aactacccca cttgcaacta taaacggcgg agagctggcg gagtttcttt ctgaaacaaa
     2161 aaatgcttat cagatcaaga caaatgatgg ctatactgga tatttagccc tcgaagatgg
     2221 aaccaaagtc acaaagaata tacaaacaaa accggaagag ctatctgatg cagtgattgt
     2281 attagatcct gggcatggcg gaaatgatac tggggcatta agcaatgatg aacaaacaga
     2341 agaaaaagat ataacattaa gtacagccat aaaagtaaaa aaggcgctag aggatgctgg
     2401 agcaacggtt tatttgaccc atactacaga tgaattagta cagcttggag atatatgcga
     2461 ctacagtgaa gaaaaaaagg cagatgtctt tatcagttta cacgcagact ctacggaata
     2521 tgcgaatgaa gcaactggaa tcacgacata ttattactat ggacaagagg aaacattagc
     2581 acagacaatt gctgactcct tcactgatct gcctctggat tcaagaggaa tctccactgg
     2641 gaactatcaa gttcttcgag aaaatcttca accttctatt ttaatcgaga tgggttatat
     2701 gaataatgat agtgatttaa aggaattagt aacagacagt tatcagcagc agattgctgc
     2761 aagtttgaca caagcattga ccacttattt tcagtaataa ataagcccag ccaaacaaaa
     2821 atagctcctc atatttttcg tttggccggg cttattatcg cagatgcaag cttttgaaca
     2881 ccgtttaagg aagaggttgt gagcctgccg tttaactccg cgtcacaagg agcaatttca
     2941 aaaaacagct tctcatgttt gatgactatt gtgacttgtg accgaggagt tggcatgaga
     3001 acaccgttta ttcggaacaa gggactgtga catgactttt gtcacagttc cattattttt
     3061 ttagcagctt caaagctttt tctgtattgg caaaatgaag tttggcatat ttttcaagca
     3121 taggcatgcc gcctttctcc aatagttttg ccgctacttg gtcagatttc gttccagctc
     3181 ctgatggaag ggtgattcca gcttcagctg atgctttgtg taactcttcc aaaaagctgg
     3241 cacgactgat gatagaagca gcagcaacag ctaaatgata ttgttctcct ttagtaacga
     3301 agtacagttt ttcttgtaat tgattttttt ctaaacgcgc atatttttta aaatttgctt
     3361 ccggcgtaaa ctgatcgatc aaaatcgctt ctggtttttc tggctcgatt ttttttagca
     3421 gcaaatggat cgcttgattg tgcaaagcga ctttcatccg aacggcattg tattttggtt
     3481 ggatctcatt gtatttttct ggagataaga caagcagttt ataggggatg gtttttttta
     3541 agatgtcagc aagctggatg atttggctat cttttaactc ttttgaatct ctaacaccaa
     3601 gtttacgtaa ttctgcaagc tgatcacgtt tcacataagc agcacatact gttagcggac
     3661 caaaatagct gccatttccg acctcatcac tacctaaaac ggataattga ctgatatttt
     3721 ttggcaaaga agaggctgtt tttgctggct tggcttgttt atcctgtgcc tccccccatt
     3781 tatcagcttc tattgctgca ttcgtacctt gaaacatcac ttttccggat gtataagcgg
     3841 taatggtcgt cccgtctttt tttgcagcaa agctagtata aggaatctgt ttatttaaca
     3901 aataagagcg atagtatgct ttcatttgac taagttggtc gtttgttgct ttgataatga
     3961 cgttgctcat aaaattctcc tcgtttttat acataaaact ataccgcaac tcacgacttt
     4021 tcgttttcta agagattgcg agacatagca tttttttaaa gtttatcata taatagttaa
     4081 gtatgtgaaa aatatttgtg gagaacggtg aaagaaccaa cttcatggta cactaaataa
     4141 aactttcatc aaataggagg aaatgattcg tatggcgcac gaaaaaacac gctacaaagc
     4201 agtgattgcc aaccaaacat atactattat cggacgggaa accaagcatc atatggatat
     4261 cgtaacgaag ctgatcaatg aacagttagc agaattgaaa caattatcac cacaaatgga
     4321 taatgaacaa gcagcaatct tgatggctgt caatgcgctt tcggatcaat tgaagaaaca
     4381 agaaagaatc ttggagctgg aagaagaaac agcagaatta aagaaaaaaa tgatcaaatt
     4441 tacagagctt gaaaatcgtg tgaaacgcat tgaagcaatc gagaacgaag ctcgggaagt
     4501 attgaaagaa aatggtcaag cagatcatac cattaaaaat catgtggaag cacaacagat
     4561 tttaaacgag aaacgaaaag gacaaattaa acaaaaatct tctaattaag atccttttat
     4621 caaaataaga aaaatttaaa aaagttagaa gagaaaggaa cgtagcatgc tgagtttgct
     4681 gattttattt ttattgctaa ttgcgttttt ttctggagca cgtcgcgggt ttgctttgca
     4741 aatcatattc gcgattggat atgtattgtc atttattgct gcccagcatt tttacaaacc
     4801 attagcaagt catttggaat tatacattcc atacccagct gtcaccccaa catcaaagtt
     4861 agcatttttt gatcaggtat ttgcttttca cctagatgaa gctttttatg caggaactgc
     4921 ttttctgatt attttgctaa ttggctggct gcttacccga tttgtggggg tttttgtcca
     4981 tggattgacg tatgtgccga tcttgagaca ggtagactgg attgcaggag gcatcctgtc
     5041 actaatcatg gcttacgtta ctatattttt gattttgcag ttacttgcct ttgttccatt
     5101 agatgtggtc caaaatcaat tcggcgagca tagtttcgct cgttttatcg ttgagagaac
     5161 gccatttctt accaacaaga tgtatgagct ttgggtaacg aatattttga attaaggaaa
     5221 atagtgctgg aaaatatttc gttttttgta cagagtagtt tatttaagaa ggctgcgtag
     5281 cgtatgtccg gcacagctct tttttcatag tgaaagataa ggtgataaaa tgaataaccg
     5341 aatccttgaa acattggaat ttgaaaaagt caaacagatg gtcagacaat ttgtagtgac
     5401 tgcacaagga aaagaagaac tggcagaatt ggtaccagtg agtgagaaac aaacgatcac
     5461 aaactggctt caagaaactg aggatggatt gaaagtgcaa cgtctgcgag gcgggatccc
     5521 tatacctaaa cttgaaaata tccgtcccca tatgaaacgg atcgagatcg gagcagactt
     5581 gaatggagtg gagcttgcac aagtttccag agtattgtct acgacaagcg aattgaatcg
     5641 tttcattgat gatctttctg acagtgaaat cgaatttgca cgtttgtata tgtgggcaga
     5701 tcagttagtc acgatccctg ttttgagccg ccgtttaaag gaagcaatcg atgaagacgg
     5761 acgcgtgact gatgacgctt ctcctgagtt aaagagcata agacagaaca ttcggcgaag
     5821 tgaacaagct gttcgtgaac agttagatgg gatcgtccgt gggaaaaatg caaagtattt
     5881 aagtgatgcg atcatcacca tgcgaaatga ccgctacgtt atcccagtca aacaagaata
     5941 ccgcggtgta tttggcggtg tagtccacga tcaaagtgct tcaggacaga ctttatttat
     6001 tgaaccaaaa caagtggtgg atttgaataa tcgtttgcgt caataccaaa tcgctgaacg
     6061 aaatgagatt caacgtattt taagtgaact ttctgctgaa cttgttcccc atcgtcaaga
     6121 aatcatccac aatgcttatg tgattggtaa aatggatctg atgaatgcaa aagcacgatt
     6181 tggtaaagaa gtaaaagcca tcgtgccggg aatcagtgaa gacaatcatg tcgtcttgaa
     6241 acaagcaaga catccactga tcgatcagga aaaagtagtt tctaatgaca tcacaatcgg
     6301 aaaagactat caagccatcg tcattacagg acctaacaca ggtgggaaaa cgatcacgtt
     6361 gaagacttta ggtctgttac aactgatggg gcaagcagga ttaccgattc ctgcaggtga
     6421 agaaagtcaa atcggtattt ttgaggaagt atttgcggat ataggagacg aacagtcgat
     6481 tgagcaaagt ctatcgacct tttcttctca tatgacgaac actgtagata ttttatcaaa
     6541 agtcaatgaa aaaagtctgg tattatttga cgagctcggt gcaggaacag atccgcaaga
     6601 aggtgctgct ctagcaatcg caatcttaga tgatcttggt aaaaagtctg cttatgtgat
     6661 ggcaacaacg cattatcctg aattgaaagt ttatggatac aatcgggcaa atacgattaa
     6721 tgccagcatg gagttcgatg tggatacact aagtccaacg tatcgtttgc tgattggcgt
     6781 tcctggacga agcaatgcgt ttgaaatctc tagccgatta ggtcttgata cagaagttat
     6841 tgatgaagcg aagcagttga tgaatgatga gagccaagat ttaaacgaaa tgatcacaga
     6901 tttagagaat cgccggaaaa tggcagagac cgaatatctg gaaatgcgtc attttgtttc
     6961 tgaggcacaa gaacttcacg atgatttgaa agaagcttac agctatttct ttgaagaacg
     7021 tgaaaaagaa atggaaaaag caaagaaaaa agcaaacgaa gtcgtgtcag aggcggaaga
     7081 aaaagcagaa aaaatcattg ctgatattcg taaaatgcaa cagcagattg ggcaaggaaa
     7141 tgtgaaagag catcagttga tcgatgcaaa gacccaatta gcaaatctac atcaagaaga
     7201 aactttgaag aaaaataaag tattgaagaa ggcgaaagaa caaaaaacgt tgaaaccggg
     7261 agacgaagtg ttggttacga cttacggcca aagagggacg cttttacgta aaaacgggaa
     7321 tcagtggcaa gtagaaatcg gtatattaaa aatgaatgtg tccgaagacg agctgacgcc
     7381 tgttgctcct caaaaagagc cgacacaacg agttatccat gctgtacgat ctgaatctag
     7441 tagtcacgtg ccgaatcaat tggaccttcg aggaaaacga tacgaagaag cgctaagtga
     7501 ggtagatcaa tatttggatt ctgccattct agctggttat cctcaagtga ctattgtcca
     7561 cggaaaagga acaggcgcac ttcgcaaggg aattactgat tatcttaaaa accatcggag
     7621 cgtcaaaagc tttgagtttg caccggcaaa ccaaggcgga aatggtgcga caattgttaa
     7681 atttaagtaa agcttactaa aggtaagcag atggttcgct atccaagtat agaaatgcta
     7741 tactaatcat agattttaaa aatggaggtc tttgatatgt cacaagtaat tacagataag
     7801 acatttaatg aagaaacaga taaaggactt gtattgattg atttttgggc aacttggtgt
     7861 ggtccatgtc gtatgcaagc accgattttg gatcagttgg aacaagaata tgatgaagaa
     7921 gaattcagaa ttgctaaaat ggatgtggat gaaaatccag aaactcctca acaattcggt
     7981 atcatgagca ttccaacatt aatgttgaaa aaagatggtc aagtagtaga aaaagctgtt
     8041 ggtgttcatt caaaagaaca actacgtcaa atgatcgatc aatatttata aaaacttaca
     8101 gcaaaaataa gctgagggtg ggactgaatc cagttccgtc ctcggttttt ttgcggttga
     8161 gttaaggtta ttcaaattaa tattttttga gtataattac gttgtcaggc tggattgttt
     8221 ttttgacaga catgctataa taactttaat tgaatatgaa ggggagtacc agatgaacga
     8281 acgaatcaaa aacaaattag ctttacttcc tgatcagccc ggctgttact tgatgaaaga
     8341 taaaaatgga acgatcattt atgtcggcaa agccaaaata ttaaaaaatc gtgtccgctc
     8401 atattttact ggcagccata atacgaaaac ggaacggctt gttagtgaaa tcgaagactt
     8461 tgaatacatc gttacagaat cgaatatcga agcattgctc ttagagatca atctgataaa
     8521 gaaaaatgat cctaaataca acattatgtt aaaggatgat aaaacctatc ctttccttaa
     8581 gataacgaat gaaaaatatc cccgtttggt gattactcgt aaagtactaa aagacaaagc
     8641 actgtatttt ggaccatatc ccgatgtcgg tgcggcgaat gagacaaaga agatcctcga
     8701 tcgattattt cctctgagaa aatgcaaacc tagtcagacg aaggagcctt gtctttacta
     8761 tcatttggga caatgtctgt gtccttatta tttcgacgta gatccgaaag tctatacgaa
     8821 aatcgttgat gaagtgaaaa agtttttaaa cggaggacac gaaaaaatcg agtcagagat
     8881 ccatcagaaa atggaaaaag ctgctgaaaa catggagttt gaaaaagctg cagaataccg
     8941 agaccagatc cgtgccattg agacaatcat gactcgccaa aaaatgacaa atactgattt
     9001 actggatcga gatgtatttg gttatgcgat tgacaaagga tggatgtgtg tccaagtgtt
     9061 tttcgtaaga caaggaaagc taattgaacg agatgtttca atgtttcctt tctataacga
     9121 agcagaagaa gatttcttga cttacctcgg tcagttttat caggaaaatg agcatttcat
     9181 tcctaaagaa gtcttgattc ctgatacgat cgacaagccc agtgtagaag ctttgttagc
     9241 tacgaaagtg ctgcaaccaa aacgaggaga aaagaaaaaa ttagttgaat tggcgaataa
     9301 gaatgcctca gttgctttga atgaacggtt tgatctgatt gccagaaaac aggaacggac
     9361 gatcggagca gttgaacgtt taggaaacgc gatgaatatc ccgaccccaa tacgtatcga
     9421 gtcttttgat aactctaata tcatggggac gaatcctgtg gctgcgatgg tcgtattcat
     9481 tgatggaaaa ccggcaagaa aagaatatcg gaaatataaa ataaaaacag tcaacggacc
     9541 ggatgattat gcatcaatga gagaagtcat ctatcgcaga tattcacgag tgattcgcga
     9601 agggttgcct ttacctgatt tgatcttgat cgatggagga aaaggacaag tagatgtggc
     9661 aaaagatgtc ttagaaaatc aattaggaat cgatattcct attgctggca tggcaaaaaa
     9721 cgataaacat aaaacaaatg agttgctatt cggtatagat ttggctgtag taccgcttga
     9781 gcgaaattca ccagagtttt tcttactcca gcgaatccaa gatgaagtcc atcgattcgc
     9841 aatcactttc catcggcagc tacgcagtaa aaacagtttt gcttcccgat tagatgaaat
     9901 tgaagggtta ggaccaaaac gaaaaaaaat gctgcttaaa gagttcaaat cgttgaaaaa
     9961 tatcacagca gccacgacag aagaattaca agaaatcgga ctgccgaaaa atgtcgcaca
    10021 aaacgtattt gataaattac atcaggaatg aggaaagggc actttacaaa gaaagtaaag
    10081 tgtcctatca ttttatgtac aacaaaaaga gcgtttacgt tttgttggta aataataaat
    10141 cgcttacgtc tggtgtctaa ggatatatgt gatttcttga agaaggagtg aagaaatgtt
    10201 agaagtcaag gatcttgtta aaacttttgg gaactacaca gctgtcgatc atgtctcttt
    10261 ccaaatcccc gatggaaaaa tcatggggct aattggccaa aacggggctg ggaagacaac
    10321 gacttttcgt ttgatcttgg attttttgac acaagaccaa ggagaagtgt tatggaatgg
    10381 ccatcatctt ggggagaaag attatgatgt catcggttat cttccggagg aacggggact
    10441 ttatccgaaa gtaacgattc aagaccaact gctttatttt gcagaactta gagggaaatc
    10501 aagaaaagag attgaaccaa aaatcgattt ttggatggaa aaatttcagg taaaagggaa
    10561 aaagacggat aaagtaaaat ctttgtctaa aggaaatcag caaaaagtcc agctgatcgc
    10621 cacgttaatc catgaaccta aattgatcat tttggatgag ccattcagcg ggttagatcc
    10681 ggtaaatgct gaattgctaa aagatggaat attggaattg aaaaaacatg gttcatgtgt
    10741 cattttttct agccacaata tggataatgt agaaaaaatc tgcgatcatt tgatcatgtt
    10801 gcgaaacgga aaaatggttt tgaatggaaa agtacatgag attcgtgaat catttggccg
    10861 tacgaagtta ttcttggaat ctggtttaag ccaacaggaa gtagaagaaa tcgaaggcgt
    10921 gaaaaaagtg atccttcatg aagacaatac gttagagatt accttagaag atcctgaagc
    10981 tgggaaagaa atctttactc gtgcgactca gttcggctac attcctatgt ttaaccagca
    11041 accgccaact ttggaagaga ttttcaaatt gaaagcaggt gaaatcgatg cgtaagtttt
    11101 ggattattac caaagatgtc tatttaaaaa atgttaagtc gatttctttc ttgatcatga
    11161 tattagtccc ttttatcatg ctgggtatca tttatgtagc aggaaacttt gctcaacaaa
    11221 atagtgatgt caataaaatc ggcgtattgg ctgataatga gcaaatcagt cagcaaatga
    11281 gccaagtcaa aacagatgat tttgagttca aagcagttga ttctgaagaa aaagcacaaa
    11341 aagagctttc ggaagaaaaa atcgatgcgt atttagtagt ctcaactgag aatgataaga
    11401 taaatgggat gctttatagc gaaaattcat taggacaaag tacacaacta ctgatacaac
    11461 aacaactgac tggcttgcaa tcgatgcttc gagcaagtca attaggcatt tcaccagaag
    11521 aagtagctag cttgagccaa caagcaagtt tgactcgaca aaaagtcaat tttgattcaa
    11581 aaggaaaaat gacagttgga gaagataact cgacagtcca atatgtcgta agttatgtag
    11641 caacgattgt cttgttcatt attattttga cgtatgcgca gatcattgca caagagatcg
    11701 cttctgaaaa aggcacgcgg atcatggaag tgatcctgtc cagtacgact gcacaaaagc
    11761 atttttacgg aaaactaaca ggtgttttac ttgttgcatt aacgcaaatg gcattatacg
    11821 gtattatttt tggtatagga tacagtcaat tcaaagatat ggaaatggtc aaaaatatat
    11881 tagacggtat ctcattggat aacttgattg gaccattctt gtggtattct cttctattca
    11941 tgatctttgg cattttgatt ttttctgttt tagcagcttt atgtggctct ttggtcaaca
    12001 aagcagaaga tacagcaaaa gcaattttgc cagtgactta tctatctcta ggcggatata
    12061 tgcttggatt gatactagga gcttcagatc caaacaatat cgttattcgg atcacttcgt
    12121 atattccatt cttatcttca tatattatgc cggttcgctt agcaaatgag acagtcggta
    12181 tgggtggagc attgatttcc ttgttggtac ttgtagccgt aacattctta ttgatgatct
    12241 tgtctgcaaa catgtacaaa tcgaatgtac tcgtgtatag cgaaggcgga ttatggagtt
    12301 cattgaaaca atcgatttca attatgagaa atgaacgaaa aaaagcataa aatatagaaa
    12361 aatatagaag gaagctggac atttattgtt cccagcttct tttttgtata gacctatttc
    12421 tattttttta ttatattgga tatatatatt cattgaaaat agagcataac aactttataa
    12481 tgttgagtat accaattttt ggaggcgttt taatggaccg ctatttagat ttactttcag
    12541 agaagttccc tacaacggaa gatgtcatca cggaaatcat caatctcgaa gcaattttgc
    12601 agcttccaaa aggaacagag ttatttttga gcgatataca tggcgaattt cctgcttttg
    12661 atcatattct gcgtatcggt tcaggaaatc ttaaagaaaa gatcaaagac cttttctctg
    12721 aacaaatgac ggaaacggag atcagccaat tcactatttt tacggcttat ccagaatatg
    12781 tgttgacgac tgattggtac aaaaaacaag ataaatccca gctgatccat cgtttgatcg
    12841 atcttttacg gtttacgaca gtcaaataca ctcgatcaaa agttcgaaaa ggattgccga
    12901 aagaatatag ctatatcatt gaggaactct tgtatatcga tgatcgtatc aacgggaaaa
    12961 aagactatgt aaaaaaaatc attgaacaac ttgtcttgat gaaggaagaa gaaaaatttt
    13021 taacaaaact tgcgcagacg atccaaaaat ctgtcatcga tcatttgcat atcgtgggtg
    13081 atatttttga tcgaggaact caagcagcca aagtaatgga tcgaatcatg gatttttcct
    13141 ctgtagatat ccagtggggg aatcatgata ttttgtggat tggtgcgtat tgcggctctg
    13201 aggcatgttt attgacgctt ttacggattg ccgcacgcta caactattta tttgaattag
    13261 agaaagaata tggattaaat ttacgtccat tattttcttt tgcccatcaa acctatcaga
    13321 agaatccagt atttacccct aaaaacagga aagatctatc tgaaagagag caagaagaat
    13381 tagaaaagat ccatcaagca ctttctatcc tccaattcaa atcagaacat cagttgttga
    13441 atcgacgacc tgaatttaaa atggacgatc gtaaattgtt ggaaaaaatc aactatgagg
    13501 aatcaacgat cgttttagaa ggtcagctct atgagttgaa agggacttgt tttcaaacga
    13561 tccagccaga tgatcctaaa aaattcttgg aagaggaaca aaaagtgatc gatagtttga
    13621 tgtattcatt ccagcactcg ttaagaatgc aaaaacacat gacgtttttg atcgaaaaag
    13681 ggggaatgta tctcacgaac aatcagcatg tactgttcca tggctgtata cctgtagatg
    13741 aaaaggggca gctcttagca tttgaattag accaatctta tcgcggaaaa gaactgctgg
    13801 gattttttga aaaacagata actgaaagcg caaaagattt gactgcaaat gaagatttgg
    13861 cgaccgattt gatttggtat gcgtggagcg ggccattttc gcctttattc ggcaagagta
    13921 aaatggcaac ttttgagcgc tattttctca cagagcctca tacgcacgtc gaaaaaagca
    13981 atccctatta tcacttgcga gatgaagagt ggttcagtga aaaaattttg gcagaattcg
    14041 atgcaaaaga agaaggttca gcaatcatca atggacacac gccagtaaaa gtaaaaaaag
    14101 gagagtcacc gatcaaagca aacggaaaag catttgttat tgacggaggt ctatccaaag
    14161 cttatcaaaa aacaaccggg attgctggct actcattact atgcaattct tacggtttcc
    14221 aaatcgtgac acattatccg ttcttgccta ttgaacaaca atttgcaaaa aaatctgatc
    14281 aaacgaatgt aaaaaaagtg atcgagcagc agctcccacg caaacttaat cggaatacga
    14341 caaaaggaat ggaattacag caacagatta ctcaactcca aaggctgttg gattatcgaa
    14401 aggacgattg agaaaaatgc tagccaacat tgctcaatag actaaaaagc gactgcaaca
    14461 aaaggcgtgt cgcagtctcc ttcggaatta gctcaaaaaa cggtaaaata ttagaagcta
    14521 tttttctgtt tatttctcta atactcaggt cagggcgctg atttcacgac ctcttcctta
    14581 aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatccaacaa acatgagaag
    14641 ctgttttttg aaattgctcc ttgtgactca gagttgaacg gcaggctcac gacctaatca
    14701 gaaaaacgat taaaattcat tcacaaagaa atcgaataaa cgctgttcag atgaatctac
    14761 acgatgagaa agttccggat gccattggac accaaggatt ttgtgcatat tatctttact
    14821 ttggactgct tcaatgagtc catcgttgga aaaggcgatg gcttttaaca aaggagaaag
    14881 atctttgact gcctgatgat gataggaatt cacaaagtat gaatcaggga gaaggtcact
    14941 taataaactg tccgaaacga ttttgacttc gtgagttgca aactgcgggg cagtgggctg
    15001 ttgttcgtgt tttacggacc attttggata taatgaaaga tcttgataaa gtgttccctc
    15061 caacacgaca ttcaatagtt gaagtcctcg gcagactgca aaaatcggtt ttctttgttt
    15121 caatgcttcc aagatcaatg cctgttcgaa ttggtctcgt tgaatatttg tttccgtcag
    15181 ttttggatga gggtcttcca tgtaaagttg cggactaaca tcctgaccgc ctgcaagaat
    15241 gagtttgtcg attttctcaa ttagttgagg cgctgaatct ggagacatga ctggcagaat
    15301 aagaggtaat ccttgagcat ctaaaactgc atccacaaat ccctgcggtg tatagcttac
    15361 ttgatttcct tggaaagtat ctgtcgctcg aatcagttga ttcccggcaa tgccgatgat
    15421 tggtttcatt tttattcccc ctaaagtaga ctaattgtaa caaggattta aaaaaattta
    15481 aactatgtaa gacttgcccc tatacttatc tgttcttgct gtgttactat ctgtaaagag
    15541 aattttcgtg tgaaattcat gacgatagtt gaaaatattg cttaatttgt tataataaaa
    15601 agtaagcaga aataaaaagg ttgggcgtaa gcttcaagga tctagttctt caaggggtga
    15661 gaaggacatt ttattgaagg ggcctgccta tactaagtta tctttagagg tacatacttt
    15721 caatgaaaaa agaaagcatg tacctctttt ttaaatcgaa aggaagtgaa gcaagtgtta
    15781 gaagctttgg aacaaatccg aaaagcagaa gaagaaaatg agaagcgtgc tcgaacccta
    15841 cggcagcagt tgaagaccta tgcagaagaa aaagagcaag aattggagat aatccaaacg
    15901 aacagtcggc aacagctgaa acagttggta gaagaaaaag aacaattcga aaagcaagaa
    15961 ttagaaaaag aaaaaatgaa attgaaagca gaagcagaca aggtgcaagc aactttgaaa
    16021 gaccaatatc aaaagcatca gagtcaagca atcggtgcaa tcattgaaag ggtgaaagaa
    16081 acttatggcc gtcactaaga tggaaaaagt gaccctcatt tcagataaaa gaaatcagga
    16141 gaggatttta caagcagtac aaggaatcca aaatgttgaa atccgggatc tgtatcaaaa
    16201 tagaacaaac aaccagtggg tagaaaaata ttttccagat accacgatta ttgataaaga
    16261 agcaaaatta agcgctttag gcaatcggct gaaagacatc caagaagcta tccaattcat
    16321 acaacaccac ggcgatagcc gtcaaaagaa actccatttg aaacgtcaag aactttcctt
    16381 acaagacttg gaagcaaatt attccgaaga aaatttcatg aataaattag aagaagtcct
    16441 tgatttaaaa gaaaagtggg aacagctaac tgaaaaacgt gatcgtttag aagaagagga
    16501 agaatggctt cttcattggc aacagctgga tattgctcca agtatgtatg caagtaagac
    16561 gaccattttt gagatgggga ctgtgagtgc agcaaatttt gaaagtttca aggaagaact
    16621 aagtaagctt gatgacgttt atatggaaga aatagattat acgccaaaac aagtttatct
    16681 ttcgtatgtt gctttaaaaa aatcagttgc tgctatagaa gaaattgctg ggaaatacgg
    16741 gttcaataaa gaaacgtatc cttatgaaca aaatcctaaa gaaaaattgc aagaaaccaa
    16801 acagcaatta tccattgttc acgagcagca aaaaaagata acgacagcgt taggtcagtg
    16861 cagcggctat atccaagaat ttgaatgggt agaagaagtc acacttgccg ttgcagaacg
    16921 ggaaaaagta aaagaacggt ttatccatgc cacttatctg atcgttttgc aaggttgggt
    16981 agatgcagaa gaaaaacaga atttgatgca tgtgttaaac gaaactgtat cagaagaaga
    17041 tatatacgtt agtttcgaaa caccaaactc agaagagatt cagcaggaag tccctactaa
    17101 attgaaaaac catccgctta tcgaaccttt tgaattgctg actgaaatgt acagtctgcc
    17161 aaaatatgaa gaagtagatc caacgccttg gatgacacca ttttatcttg tcttcttcgg
    17221 gatgatggtc gcagatgttg gttacggatt gctgatgctc attgggacaa tactagctca
    17281 aaaactattt gttttacccc gcgggatgaa acgtttcgta aaattctttg agattctctc
    17341 ggtaccatcg atcatctggg gattgatcta cagttcattt ttcggacagg cattaccaaa
    17401 agaactattt ggtatccgct tgcctttccc aatcctatct acgacggatg acgtcaatac
    17461 gattttgatt ttatccgtga ttttcggtct gatacagatc ttggttggat tattcgtggc
    17521 agcaaaggaa aatatcagga ggaaagatta cttgagtgct gtcagtgatg gttttgcctg
    17581 gcaagggatc ctgattggga tcgttattgc attgattggt gtcatgttga taaaaaatac
    17641 agcatttgtt tatcttggcg cggggctatc gatcattgct gctttatgta tcttaatcgt
    17701 gccgattatc caatcgccgt ctaaagtcaa gggtgctgcc aaaggagcgt ataatcttta
    17761 cggattgact ggttatatcg gtgatttagt cagttataca cgtttgatgg cactaggtat
    17821 ttcaggcgga agtattggcg cggcattcaa tatgctagtt gctttcatgc cgccagccgc
    17881 tcgattcagt gtagggatcc ttttgatcat cgctttacac gcactgaata tgtttttgac
    17941 actgttaagt gcctatgtcc atggtgcacg attgcagtat gtcgaatttt ttggaaaatt
    18001 ttatacagga ggcggacgtg cctttgaccc gctgaaaacg gcggaaaaat acgtcaacat
    18061 caatcataag aaaaaaaaca ataaataatt ggaggatttt tatacaatga tggattactt
    18121 aattagtcaa aatggtggaa tggtatttgc agtattagcg atggcaacag cgacgatttt
    18181 ttcagggatt ggttcagcta aaggtgttgg aatgacaggt gaggcagcag cagcgttaac
    18241 aacaagtcaa cccgaaaaat tcggacaggc tttgattctg caattgcttc caggtacgca
    18301 agggttgtat ggattcgtta tcgccttttt gatttttatc aacttaggaa atgatatgtc
    18361 agttgtacaa ggattgaact ttctaggcgc ttctctgccg attgctttta caggtttgtt
    18421 ttcaggtatc gcccaaggga aagttgcagc agcaggtatc caaattttag cgaaaaaacc
    18481 tgaacatgct acaaaaggaa tcatttttgc tgcgatggtt gaaacatatg cgattttagg
    18541 tttcgttatt tccttcctat tagtattgaa tgcctaagca taaaggagga aaagcagatg
    18601 aacgccattg aaaaaatcat ctctcagatg aatgaagcag cagagcaaga aagagcggcg
    18661 ttagagcaag aagagcgaat gaagatcgat caaaactttg aacaaaaacg tacgcaaatc
    18721 gaaacagaac atcaaaaaca aaaagaaaaa caaatcgaat tacttgaaaa aaaataccgt
    18781 cagcttagga atcgccaaca agtagaagta cgacaagaaa atttgaatgc gaagcaggaa
    18841 tttttacgac gattgtttgc agatgcaata gctgaaatgg agaattggga tgaatctgaa
    18901 caaatccagt ttatcaaaaa ttcattgtat tcattacctt tgacaggcaa agtaacattc
    18961 attgctggag aaaaatcagc agcttatctt tctcagacat tgctggatga atggaacaat
    19021 gaacttccct ttatgatggt tttaagtgat gaaactgttg ctgatcaagc gggttttctg
    19081 ataaacgatc aaggagttca gtataatttt cttttcagca gccttgtaca agatatccaa
    19141 ggaacgatga gttttgagat tgccaatcaa ttattcgaat aaggagggat cagtgtatgg
    19201 aataccacga actaaatccc ttgattcgcg gaagagagct tgaacttttg tccaatgaga
    19261 catttgaacg gatgattcaa acaaattctg tcgaagcact tggtgaaata ttgaaatcca
    19321 cgatttacag tccatatatt tatgacggat atgaatatga cttcgaacag aatcttttaa
    19381 aggagcagag ccgtctattt cgctggttaa aggaatcagc accggagcct gaaatcgttt
    19441 ggatctatac gatgcgctat actttccata atctgaaggt tttgacaaaa gccgagatga
    19501 caaatcaaaa tctggaccat ttgtatatcg acgatggatt ttattcatta gaaaccttga
    19561 aggatgccat tcatacgcaa gtttcaaatg aactccctga aagcattatg gacagtatac
    19621 gagaagtacg tgattatttt gaagagtcta ccattcttca gggaattgac gtgatttatg
    19681 atcgacagtt tttgacagag cagcgacggc ttgccgataa gctaggatat caagaattgc
    19741 tgacagaagt cattgctttt attgacttga ccaatatcac aacgatggct cgaggaatat
    19801 tgcagaaaag aacatatggg ttcatgacga cggtactttc cagctcggga agtatcgaaa
    19861 aagaagtatt tttgaacttt gtagataaag atatggaaga ttacacgaat tttctattag
    19921 agacagatta tgctgatttt ctgcgccctg tcatccgtga gggaaatctt gatctcgtaa
    19981 agcttgaaca gttgaaagat gattatctaa gttcattgta tcaagaagca caaacccagg
    20041 ctttcggacc actgccacta ctagcctttt tgaacgcaaa agaggtagaa agcaaaaacc
    20101 ttcgtttact ggttatcggc aaaagaagcc atttcgaacc agaaacaatc agaaagcggg
    20161 tgagacaagt ctatgacgca taagatcggt gtagttggcg acaaagattc tgttttgcca
    20221 ttcaaattgt ttggctttga tgttcgttat ggtacaacca agcaaaccgt tcgtcaggca
    20281 attgaagaaa tggcaaagaa tgagtatggc gtgatttatg tcacagagca atgtgcagca
    20341 ttgagcccag aaacgatcga acgttacaag gaacaaatga tacctgctat cgtattgatt
    20401 cccaatcatc aaggatcatt agggataggg ctggaagaaa ttcaaaaaaa cgtagaaaaa
    20461 gcagtaggtc aaaatatctt atagtatgaa aaggagatag aaattttgca aattggaaaa
    20521 atcataaaag tctccggacc tcttgtaatg gctgagaaca tgtctgatgc cagtatccaa
    20581 gacatgtgtc tagtgggaga tctaggagtt atcggagaaa ttatcgagat gcgtggcgat
    20641 gtggcgtcta ttcaagtata tgaagagact tctggaattg gtccaggcga accggttcgc
    20701 tctactggtg aagctttatc tgttgaatta ggaccaggga tcatttcaca aatgtttgac
    20761 ggaatccaac ggccattaga tacctttatg gaaatcacac aaagtaattt cttagggcgc
    20821 ggagttcaat tgcccgcatt ggaccatgag aaaaaatggt ggttcgaacc aacagtagaa
    20881 gccggagaaa cggtgagtgc tggagatgtt atcgggatcg tagaagaaac aaaagttata
    20941 aaacataaaa tcatggtgcc aaatgggata aaaggaacga tcaaaacaat cgaaaaaggt
    21001 tcattcacta ttgatgaaac agtatgtgtc attgaaaccg aaaatggtga caaaaatttg
    21061 acgatgcttc aaaaatggcc agtaagacgt agtcggccaa tcaaagaaaa attaaacccg
    21121 gatgcaccca tgatcactgg acagcgagtg attgatacat tcttcccagt gacaaaagga
    21181 ggagcagcag ctgtacctgg tccttttggt gcagggaaaa cagtcgttca gcatcaaatt
    21241 gcaaaatggg ccgatgtgga catggttgtc tatgtgggat gtggcgagcg tggaaatgag
    21301 atgacggatg ttttaaatga attccctgaa ttgattgacc ctaatacagg agaatcgttg
    21361 atggaacgta cggtattgat tgctaataca tcaaacatgc cagtagcagc tcgagaagct
    21421 tctatctata cgggaatcac aattgcggaa tatttccgtg acatgggtta tgacgttgct
    21481 atcatggcag actccacttc acgatgggca gaagcacttc gtgagatgag cggacgtttg
    21541 gaagaaatgc ctggtgatga aggttatcca gcttatcttg gttcacggct tgcggaatac
    21601 tatgaacgat ctggaagagt cattgcatta ggatcagaag gtcgcgaagg aagtataacc
    21661 gcaatcagtg ctgtatcccc gtctggaggg gatatttcgg aaccagtcac acaaaataca
    21721 ttgcgggtcg taaaagtatt ctggggtctt gattccagtt tagcccaaaa aagacatttt
    21781 ccttcgatcg attggataca aagctattct ctttattcaa cagaagtagg gaagtatatg
    21841 gatcatatcc ttcaagctga ctgggcatcc atggtcaccg agggcatgcg tattttgcag
    21901 gaagaacagc agctgaatga aatcgtgcgt ttggtaggga ttgattccct ttcagataat
    21961 gaccgtctga cattagaagt ggcaaaatcc attcgtgaag attatttgca gcaaaatgct
    22021 tttgatgatg tcgatacatt cacttcaaga gagaaacaat tcaaaatgtt gaagatgatc
    22081 ttgacctttg gtgaagaagg acgccgtgcg ttgtcgttag gtgcttattt aagtgagatc
    22141 atggacggta ccgtatcgat tcgtgaaagg atcggacgaa gcaaatatat cccggaaaca
    22201 gaattagaaa aactagatca aattattggt gaaatcaagc aaacgatgca aacgatcgtt
    22261 tcagaaggag gcatgagcaa tgattaaaga atatcgtacg atcggtgaag tcgtcggtcc
    22321 attgatggcg gttgaaaaag ttgctggtgt caaatatgaa gaattaatcg aagtccgtat
    22381 gcaaaatggc gagattcgtc gtggacaagt attggaagtc caagaagata aagcaatggt
    22441 ccaaatattc gaaggaacaa gtggtatcaa tttacgtgac tcttctgtac gatttttagg
    22501 ccatccttta gaactaggtg tatcggaaga catgatcggt cgagtattcg atggtttggg
    22561 acgaccgaaa gataacggac cagagatcct gcctgaaaag catttggata ttaacggaga
    22621 agtaatcaat ccagtagctc gggattatcc agatgagttt atccaaacag gtatctctgc
    22681 aatcgatcat ttgaatacat tagttcgtgg gcaaaaatta ccagtatttt ctggctctgg
    22741 tcttcctcat aaagaattag ctgctcagat cgcgcgacaa gcaacggtac tcgattcatc
    22801 agatgatttc gccgtagtct ttgctgctat tggaatcact tttgaagaag ctgaattttt
    22861 catggaagat tttcgtcaaa cgggcgcaat cgaccgttct gtcatgttta tgaatttagc
    22921 aaacgaccct gcaatcgaac gtatcgctac tcctcgtatg gctttgactg ctgcagaata
    22981 tcttgcttat gaaaagggaa tgcatgtttt ggttatcatg acagacatga ccaactatgc
    23041 agaggcattg cgagagattt ctgcagcacg gcgagaagtt ccaggaagaa gaggatatcc
    23101 tgggtatctt tacacgaact tagctacttt attcgaacga gcaggaagaa tccgcggact
    23161 aaaaggatcg gttactcaaa ttccgattct aacaatgcca gaagacgata aaactcatcc
    23221 aatccctgac ttgactggtt atattacaga aggacagatc atcttgacta gagagcttta
    23281 caagagtggg attcagccac caattgatgt actcccgtct ctttcgcggt taaaagataa
    23341 gggaactgga cccggaaaga caagagaaga ccatgcagct acgatgaatc agctttttgc
    23401 agcctatgca caaggaaaac aggcaaaaga attagccgta gttttaggtg aatctgcttt
    23461 atctgatgta gacaagatct atgcgaagtt tgctgagcgg tttgaaaaag aatatgtcaa
    23521 tcaaggattc tacacgaacc gttcgattac tgaaacactt gatctagggt gggaactgct
    23581 atcgatgttg ccaagaaccg aattaaaacg aatcaaagac gacatgctgg acaaatatct
    23641 gccagaaggg aagtgagttc tgatggcacg attgaatgtg aatccgaccc gcatggaatt
    23701 gactcgattg aaaaaacagt tgacaactgc cacaagaggg cataaattat taaaagataa
    23761 gcaagatgaa ctgatgcgtc aattcatcct gttgattcga aagaacaacc agctgcgtca
    23821 agaaatggaa gagtctatga cagaagcaat gtctgatttt gtattggcaa atgcttcgat
    23881 cacggaacct ttcatcgaag aattatttgt tttgccggcg gaaaacgttg atttgtctat
    23941 cgtagacaaa aatatcatga gtgtaaaagt acctgtgatg aattttgaat atgatgaaca
    24001 gctgacagac acacctttgg aatacgggta tttgaattcc agtgccgaac tggatcggtc
    24061 aatcgatcgg tttactaccc tgcttccaaa acttttggaa ctgacagaaa tcgaaaaaac
    24121 ttgtcaactg atggcgagtg aaatcgaaaa gacacgtcgg cgagtcaatg cattagaata
    24181 tatgacgatc cctcaattag aagaaacgat ctattatatt cgtatgaagc tggaagaaaa
    24241 cgagcgcgca gaagtaacaa ggttgatcaa agtaaaaaat atgggtacca ctgactaagt
    24301 ggtatctttt tttaaaagag gaaaacgatg attaaacaaa gcaaacaaat aaagaaacgt
    24361 ttgccggctg ttcagcttct cgcattagga ttttttctcc tgattttagt tggcggcggc
    24421 ctacttaccc taccattttt tagtaatagt gggcatggga caaattttat cgatgcgctt
    24481 ttcactgcaa cttctgctgt ctgtgtaacg ggattgacaa cattgaatac agcagagcat
    24541 tggaacagtg ctggtcaatt tttgatcatg cttttgattg agatcggcgg tcttggtttt
    24601 atgatggttc cgattctatg ctttgcattg ataaagaaga aagtcagtct cagcacacgt
    24661 attatattaa aagaagcgct gaatctagaa gaagtatcag gtgtgatgaa gctcatgctt
    24721 tacatcctaa aattggcctt tgttatccaa ttgattggta cggttgcttt atcttttgta
    24781 tttgtgcctg aatttggctg gttgaaaggt atctggtaca gtttgttcca tgcaatctct
    24841 agtttttgta atgcagggtt tgatttgtta ggagacagtt tagccaatca ccagcaaaat
    24901 gtttatttga tcatggtggt atctgctttg atcatttctg gtgggttagg cttcatcgta
    24961 tggcgtgatt tactggaata tcatcagaaa aagaagatga cgattcattc aaaaatcgca
    25021 ttaaccgtta caggtaccct tttattaggc ggattcgttg tgttcttttt cacagagcgc
    25081 aatggggcaa cgctagtgga agggaatatg tttcaacgat tggcaaacac cttttttatg
    25141 agtgtgacac ctagaacggc tggatattat tcagttgatt acctccatat gagccatgca
    25201 ggtctgattt taacgatgtt tttgatgtac atcggcggaa catctgggtc gacggccggc
    25261 ggattgaaaa caactacgtt aggtgtgttg ctgatccaga tgcgttcgat gttgaaagga
    25321 cgtacgagag cggaagcatt tggccgtacc atccgacaag gcgcagtgtt gcgggcactt
    25381 actttatttt ttgttacgtt gacgttgtgt gtagtcgcca tcatgatttt atcggtgact
    25441 gaaacgatac ctaaaacttc tggcatcgaa tatattgctt ttgaggtctt ctctgcgttt
    25501 ggtacagtag gtcttacgat gggattgacg ccagacttga ctgcaatagg gaaattgatc
    25561 attatagcgc ttatgtacat tggacgagtt ggtgtattga ctgtggtctt ctctctgatg
    25621 acaaaagcac aaagaacaca atcaaaatac aaatatccag aagaaagtgt catgattgga
    25681 taatttcatc ttctgacaga aacctagtga tttagtaaaa taagaaaata aaataatttt
    25741 tcttgcttct tggatacttt aagtgcaaaa tgttcgttta ttttttgagg cactacccgt
    25801 tgttgaacct cgggtagtgc ctctttttta ttgaaaggaa tcaataagat ttgattcctt
    25861 tatttatcaa taggttataa ttcatatttt tttatttttt cttttacgat acttagtcca
    25921 gaagctaaaa tttcaaggtt attagaggat agggaaattc gaacgaatgc atctggttgc
    25981 gcacgtgtag taaagcgtct ggaatgataa agtcggatac cgttagcaag aaagtcattc
    26041 tcaattatct tagggtcaat ctcaggatca actggaagaa ttcgatagta agggacacga
    26101 ttcgttggtt tttctacttt gaaaagagaa tcaaaaagct gattggctct ttttgtaaga
    26161 cttaatttag tttctaagat cctatttgct tcatttgaat ggaggatttg agtgatgact
    26221 tccacatcta atccagaagt tttcacattt atattaaaaa ttgcttgttc tatcttgctt
    26281 tggacatgta ctggaactac aagataagca actcgcaatc cagaacaaat aaattttgtc
    26341 attccagcta agtagattgt ttggttaggt aataaatttt ggaaaggagg aatgatcgta
    26401 tgttgatagt aagttgttaa aaatgaatgg atatcatctt caataaccca tagaccttct
    26461 ttttcaatca cttcggctaa ttgctctcgt cttttgtgag agatttgaaa ccctatagga
    26521 ttattacaag aaggcataag aaagacacca tgaatcttct tttttctaca ttcctgaatc
    26581 aatatatcag gtcgcattcc ttgctcgtca aaatcaattg gaacgatctc taaatgatat
    26641 aactgagcca attcaatgaa attagcgtat gtatatcggt ctgttgctat tctatcacct
    26701 ggagagaaaa gtgcagaaag tgtgatagcc aatccatttt gaacaccgga aacaatagat
    26761 agagattcta aggacggaat gactccttga gtttttaacc aatttgctgc agtagtcaat
    26821 tgaaaatttg tgcctttggg atcttggtaa ttaagcaaac caacaagatt tgggttatta
    26881 ctagttgatt gaataaacgg gacaacgaat ttattacatt cttcaaaaga actaaccaga
    26941 ccaaaatcaa tgatatgttc ggttacttca ttggtagaga tggtgatgga ctcaaagaca
    27001 ttgggagaaa taaatgtgcc acttccaata ttcgtataca gcaaaccttt ctcaattcca
    27061 tatttataag ccttgccaac ggtagtaaaa tttaaatcta aataatcagc tagctctctt
    27121 tgggagggta actgagtgtt tttctgtaat ttattcttta aaatatcttg ttctaattgt
    27181 tgaatcaacg atcgataata aggacgcttc aatttttgtt tgtctggtcg ccaagttaac
    27241 gggtaattgt cataagaatt aataggcatt ttctccactc cttaaaattg tataccatac
    27301 aattttacca ttgattgtat tgattttaga agttaatctt ttgtgaggag ggaattgtct
    27361 atgaaaatta ttaattttga ggaagtagta gcttctttta gttttgaaga agcgattcaa
    27421 gtaatgaaaa aatgtttttt tgattatgaa agtggtaaaa tcagccaaaa tcctcgattg
    27481 gtggaaattt tacctgacgg aaaaggcaaa aatatgtttg cgatgatgcc tgcttatttg
    27541 ggggagaaca gatattttgg cacaaaagtc attaccgctt ttccggataa tcatcagtat
    27601 catttacctt ctcatttagg agagattttc ttgtttgatg ctcaaaacgg actacccaaa
    27661 gcattgataa acgcaaatgc agtcacctgg attcgaacag ctgctgtatc tgcgctggca
    27721 acagattact tagcaaatcc gcatgcagaa acacttgctt taattggagc tggtcaacaa
    27781 gcattgtcgc atttgactgc gatttctacc attcgtccca tcactacagt gtttgtttac
    27841 gatattttag aagaaagacg aaatactttt atcgctaaag ctaaaaaaca gtatcctaaa
    27901 ataacgttta taaatgcaat atcagtagaa gatgcagtac gtccagcgga aatcatttgt
    27961 acgttgacct ctagtaaaga ggcatttctt caaaaagagt gggtaaggga agacgcacat
    28021 atcaatgctg tgggcacatt tactcctgat acaagagaga taagtacatc gttgatggaa
    28081 acttctcaaa tctatgtaga tgattactca gctgccttaa cagaaagcgg ggacctgctt
    28141 atagccatgg cagaaggaga tttagaggaa tcttgtattg ttggtacatt aggcgaatta
    28201 gtttctgaga aaaaaacatt ttataaagga gaatcgaggc cgacgatttt cgacgcagtt
    28261 ggtctagcta ttgaagatct ttgttgtgca gaatatattt ataacaaatt agagaaaggg
    28321 gctttaaaat gaacttagat tattacgtag ttgatgcatt tgcagatgag gtgttcaaag
    28381 gaaatccagc agcggtttat gtgctagaag aatggctgcc agaaggtacg atgcaaaaaa
    28441 ttgccattga aaacaattta tctgaaacag catttacagt gaagaaaaac caagaatttg
    28501 agcttagatg gtttacgcca gatcgagaaa tcgatctttg tggtcacgcg acattagcaa
    28561 cagcatttgt tttatttaac tattacaaaa tacctgatga aacgattaaa ttttctactc
    28621 aaagcggtaa tctatttgtg acaagaatta aagattatta ttacatggat tttcctagta
    28681 ttatgccgaa aaaagtgccg atattgtctg aatatgaaga agcgattggg gctaagatta
    28741 aagaagccta cttagcacgt gatttatttt tcgtattaga agatgaaaaa acggtggcta
    28801 aattgcagcc agattttaca gctatcaaaa attttgaatt aggaatagga gtcatcgtga
    28861 cagcagaaag tgtgcaaaaa gactttgttt ctagaacctt ctttccaaaa ttaacaatca
    28921 atgaagatcc agtatgtgga tcagcacatt ctaatcttat tccttattgg gcagaaagaa
    28981 tgaataaaac aaaattgtca gcttatcaaa tgtcatcaag gggcggggat ctagaatgtg
    29041 aattaaaaga agatcgtgtg ataataggag gaaaagcagt tttgttttct aaaggtacct
    29101 cgtttatttg atttctttca aatgaattaa aaataaattg gtggagagtg gcaaccttat
    29161 agctacaatg gttgttaaaa aaagacgcaa actttttcaa tttaatgaaa agtttgcgtc
    29221 tcattttaat tattggtgtt tgttttttat tcaatcaaga gtaaaatgcc ccatactaac
    29281 tatcctcgga tgacttcgat tttataacca tcagggtcaa tgacaaagta gtaagaaggc
    29341 gcagtgccag gtaatccttt taagtcagta atctcaagac cggcagcttt atgttgctcg
    29401 tgtaattttt ctagatcatc agctgctata gcgatatgac catagccgtc tcctagatca
    29461 tacgcaccat gatcatagtt gtaagtcaac tctaattcat agtcatcccc aggaagtgtc
    29521 aaataaacaa gtgtaaattt gttttctgga aaatcacggc ggcggctttc ttcaaaacca
    29581 aatgcctttt gataaaattc gattgatgct tcaagatctt tcactcgaac acatgtgtga
    29641 gccattttca tagattcatc tctccttttc cttttactca atcataacaa aaagaagtaa
    29701 ggaaatagtt gatttgtgct tgttcatgag aaatttctca tgtaaaatgg atagaaaata
    29761 aaactgttaa ggtggaaact attcatggat aaaccaattt ttggtgaaaa aaagcaattt
    29821 gctgattatc aaacccgtta tgcagcatat attgtcatag aaagagatca gcagattgca
    29881 gtaatcgagg ctccaaatgg tgcttttttt cttcctggcg gggaaattga aggagaagag
    29941 acgaaagaag aagcgattgt ccgtgagctg atagaagaaa tgggaattgt ggcagaaatt
    30001 tcttcttatc tgggagaagc tgatgagtat ttttactcga attatcgtgc aacttattat
    30061 tataatccag gatatttctt tgttgctgat tcttggaagc gtataggaga accaactgaa
    30121 aagaccaata aaatttggtg ggtgagccct caagaagcgc ttgtgaaatt gaaacgtggt
    30181 agccaccgat gggcagtcga gagatggatc gaaaaaaata aaaactgaaa gaaagcgagt
    30241 agatcagctt tctctcagtt tttttattta ttacagtaca tattttttga ttgcttcagc
    30301 tacaccgtgc tcgtcgtttg atgaagtgat gacatttgct gcttctttga caagagaaac
    30361 ggcatttccc attgctactc ctagaccagc atattcaatc atggataagt cgttctcgtt
    30421 atctccgata gccatgattt catcttgatc aataccaagg tgctgtgcga gattcgcaac
    30481 tgccgcacct ttacttgctt ctttatttag tacttcgaag taatagggcg cacttttgac
    30541 cgttgtgtat ttatcacgga aagtttgcgg taaacgcgca attgctgcat ctaaaatttc
    30601 tggttcgtcg atcatcatca ttttgacgat gttcatgtct ggtgtcattt cttcaggtgt
    30661 tcgatatttt agcggcatct tgactaaact tgcttcgtgg atcgtataag cgctgatatc
    30721 tcgattaggc gtgtagatcg ctttgtcatc gatcgaatgg agatgactgc cgacacggcg
    30781 tgccatcact tcgatctcta ggaaatcttc atgtgtcagt ccgtgacggg cgatgatttt
    30841 acctgatttc gtttgttgta cgagtgcccc gttataagta ataacgtaat cattatcttg
    30901 aaaaaggttc aactcgttta actgttcctg gacacctggt aagggacggc cagtgcataa
    30961 aacgatcgaa acaccttttg caacagcagc ggcaatcgct tcttttactt cttttgttac
    31021 ttctcttttc tcatttaata aagtaccatc gatgtcgata gcgactagtt tgatagacat
    31081 aatttcctcc aatatatttt aatttatttc aatcaatgcg ccattacgga tatggctggc
    31141 aaattcttga taagtttcgt caaataaatc ataatgatca cgcaaggacg gatcaaccat
    31201 ttcttttgga aagaaaaatc gctcatctcc ttgttcttgt cccgctaatg cagcaacaag
    31261 ctgactaacc tgtgataatt ctatcaaagt tccgtcattt cgcaaaatct cgatttgtgt
    31321 gcgatgtctg ccttgttctg ggcgataaaa atcataaggt agatcgtagc tagaattcac
    31381 agcagtgtaa taaacaggat tatatccgac cttttctact aacaaagtga gttcttggac
    31441 taattttgaa tcccggttgc cagaaaaagt agcagatttc agtggtttac gattcaaaaa
    31501 acgtttagct aaatcattta aaataggatc agcagattca gaccattgag taaagtaagt
    31561 gcccaagacc ccatcatcaa gtttgaggta ttcttctaaa gtaaagcttc cgtcaagaaa
    31621 tggcagaagt aattgagaat ggagttcaaa actgctggca tcttcttgat acaactcgtg
    31681 tgcgcgatga agcagatgct ccaaaattac ttccatacca cgggaaacag gatggaagta
    31741 cacttgcaca tacatttgat atcggctaac gatataatct tccactgcat gcatgccact
    31801 catggaaaaa gcgatcccgc cttcataagg acgaatcaca cgtaatattc gggtaagatc
    31861 aaatgttcca tattctgttc cagtgaaata agcatcccga agcaaatagt ccattcgatc
    31921 tgcatcgatc tgactagaaa tcatttggac aacttgtggg ttggggtaag ttttttggat
    31981 cacactagct accttttcag gaaaaccttc ttctactcgg ttaagaatct gatacacttc
    32041 tgtttctgga gaagtgatga tctctacagt gatggcttca tgattcgtat ggaagatatg
    32101 ttcaaatgta tgagagtaag gaccatgacc gacatcatgc aacagtgccg cacataaagt
    32161 caccagccgt tcctgatcat cccagcctcc gttgcctatt ttttcttttg aaaaatttcg
    32221 agaaaagata tcgcagattc gtcttgaaat ctcataaacg cctaatgaat gagtaaaacg
    32281 gctatgttct gctccgtgaa aagtgaaaga agaagtgcct aattgtttaa tgcgtcgtag
    32341 acgttggact tctttcgaat tgatcaaatc taagattact tgatgctgga catggatata
    32401 gttatggact ggatcacgaa aaactttttc cataggcagc atttgatatt tataaggggt
    32461 tgtcataatg tagctccttt atgttattac gttgttccat tctctggatt tggttgttcc
    32521 attgctccag ctggatttct ttttttagcc agtcggtaga atcctgtaaa tcacttgcag
    32581 aatacagctt ttcaaaagct tttgcaaatg cttctttgac ttcttttaca aaaaggggtt
    32641 ggtcaagcag ttcttcaagt gttgccattg tagcaggatc cactgcagga tagccatttg
    32701 taccaaagtc ttcttttaaa gcagattgat aaaatcgctt catgacttgc ccgcgttttt
    32761 cctgattacc gttgacactt aagtacatca taacagaaat cccatttttg atccgacgct
    32821 gagcgatacc ggcaaatttt ttgccatgga tgcttaaatc ataagttcca ggacaataag
    32881 aatgggtgat ttcgaatgct tgtatcggtg cctttggaaa agcgtattta gtcaatagga
    32941 gcatttgctc gtaagcgtca tctatggtta ttttttttgt gtcactttgg gggaaaatca
    33001 aagaaatatt caaaattcct tcatctgata cgatccctaa tccacctgaa tttctgatca
    33061 ctggagcgta acctgcatct ttcagtgttt ggatcccgtc acttagaaaa ggagttcgaa
    33121 catccttcat acctaagatg actgtgcagt catattgcca gaaatgtaaa aagatttgct
    33181 ggtgttcttt agaatattcc gttaatatat ctgttaatgc aaaaggggaa aagtcgtttt
    33241 tttgataaat gtgctgatct agtcgaatcg cagggttcat tcaaccggct cctttgtata
    33301 aattcatatt ttctacaata ttatactcta aaatcaaaga gttgttttgg aaaaaaggtt
    33361 ttctgtaaaa atggacaagt tggttgacaa aacaaaagaa actaggaaca atgtagctat
    33421 gagaagtaaa ggagaatgaa taatggaatt atcacaagga acaccgattt caatcaaatt
    33481 gcgtacaaaa gtaaaacagc aaggggaagt gcaagatttt tattttgatt tgaaagggca
    33541 aatggtgaca attggcgata cgctgtacat acgttataaa gagattcagg aggagacggg
    33601 cgatgagatt cctgtaacga tcaaattagt tccagatggt catgtacagc tgatccgtgc
    33661 aggagaaatg cggatgcgtt tgaaatttgg ctataaagaa cggttagaaa catcctatcg
    33721 aacaccatac ggtatgatcc agattgctac ttttacgaaa gaactgcatg ttagtttgaa
    33781 agaccgtccc acaggaggga aggttcgaat cgattatgat ctgttcatgg gaccggaaaa
    33841 aatcggcgaa tattatttga ccttggattt tacagcgtaa atataaaaga aaagtttgat
    33901 tttttgtgtc tgattttgta tgataaggag tagactgtga aaggacgtgt cctgattgga
    33961 aattagtgta tttgaaggtg caaacaaaag cgaattatca atgatcgaag tagcacacgc
    34021 gatcttagaa caacgtggcg atgtcatgga tttttctgat ttagctaacc agatccaaaa
    34081 ctatcttggg aaatcagaca gtgaaattcg tgattcactc gcacaatttt atactgactt
    34141 gaatattgac ggcagcttta tctcattagg ggataatcgt tggggcttgc gttcatggta
    34201 tgctatcgat tcgatcgatg aagaagtaaa ccatggaatg gacgaagagg atgaagatac
    34261 tccacgtcgc cgtaaacgca agaaagtcaa cgcctttatc aatgatgatg aagacgcgat
    34321 cgattacaat gacgatgacc cagaagatac agatctgaca gaagaagatg acgatgatct
    34381 gtttgatgac gatgatgatg aagatgaaga aatcgctgct tataattctg acttgcaaga
    34441 aatcggagca gatgacccga cagatgatga agaagacttg ccaggaaata tcgaagaaga
    34501 tttaagtatc atagacgaag acgatgatga tgaagattat gaagaggaag aattttctga
    34561 ctctgaaaaa gaataaacat tattttctta cgagaggttg tgacagagac gatcagccta
    34621 tcagaataag gaggtgtgac acgatttttg tcacaccttc ttttttgtat ataaaaatcc
    34681 caagtaaata cataaaaaat aaaaaaattg aaacttttag cgcaccttgt acgtcatatt
    34741 aagtgaggca gaaaggagtg gagaatgatt tttactggat ataagaaaaa acggcagatc
    34801 gaaaaatacc gtcacctgct gaatggactc atcgaaactg attatcaaaa aatgttccgt
    34861 atcacgatga attacgtaca tgataaagaa gaggccttag atgtgatgca agatagtttt
    34921 cataaagccc tgaactattt tgaaaaagaa aaagagatcg agcattttag tgcatggttt
    34981 tatcggatat tgatccgcac ggcattagat agttggcgca gacaaaaaag gacgcctgtg
    35041 gacttatttg aaattcaaga tatcccccaa cagttacctt tagaaacttc cgttgcagag
    35101 ctccatatga ttttagataa aatagacagt ccggaaaaag agattctgat cctctacttt
    35161 tttgaaggat ttcgtttgaa agaaattgcg actatcttgg atatgaatga aaacacagtt
    35221 aaaacgaaaa tgtaccgctc attgaatgct ttacgtcaaa tcctagaatg aaggagagaa
    35281 gagtgccatg tattcccaaa aagaaaattt aaaaaaacta aagaaaactt ataaagaaca
    35341 agcaattccc aaagggctta aagaggagat ccgccagcgt tttattatcg aagagcaatc
    35401 atttataaaa gcaaagcgga gaaagagacg tctccaaggg ttcggagtat ctttggctat
    35461 cgtaatgatt acaacaagta gccttctttt taatagtcaa gttcgaagct ttgcagaaga
    35521 tttacccatc ttaggatcag tcattgaatt gatcttaggc gaacgcttta ctgatcgatc
    35581 tgaaaaaatc gatattcagg taccgaagat caatacacaa aatgagaaag aaaataaaac
    35641 aattcatgga ttaaatcaaa aatactttcg cgaaggacaa gcagattttg aaaaagctga
    35701 aaaagaatat ggaaattttg aaacggatca ttaccaagtt ttgggagatt atcagaaaat
    35761 cgtagacgat aaccggtttc tcgtgattga aaggcagatc acccaaacag cagcagatag
    35821 ccatgttgaa aaaagatacg acacaatcga caagaaaaac agtgtccagc tctcacttcc
    35881 acttttattc aaagatgaca cctatctatc tgttttgaca aaagaagtca aacggcagat
    35941 ggccgaacaa gtcaaggaag atccatctaa gtactattgg actgagcaag atattcaaga
    36001 agggacgata gaaaaaccaa cacttgttac gcctacacga agtttttatt tgaacaaaga
    36061 gcatcaatta gttttgactt tttctcaata cgaaattgct cctggttata tgggtacacc
    36121 agaatttgtc attcctaagt ccgtgacaaa aacgattttg gcatcggaag attatttgga
    36181 tcattagacg agaagtagtt gcgactagca gtttcaattt tcaagtaagt acaagtaaat
    36241 aataagaaga gaaaaagaga aagaagagat atagtgaaaa gaaattattt agttatcagt
    36301 attattgctt tatccgtgct tttgtttttc gaaattactt cggtggcctc cttgatcacc
    36361 gaagtaaaag gacaaacaca ctcttcggag cattcgtcac aatctttcaa acaatcagaa
    36421 caatcaacca ttaaaagcca atcaactgaa acagctaacg tcaaagaagc cattcaagaa
    36481 tcagcggaaa aggtacaaag tcagtcatca caatcaacac aagacagtac aaaaacgagc
    36541 cgagatccgg ctgtcaaaag cgtcgcaatc agctttgatg atggaccagg agcgaccaca
    36601 acaccgcagc ttctgcgaat cttaaaagaa aaaaacgtgc atgccacttt tttcgtctta
    36661 ggagaaaata cggcgcagca tcctgaaatc gtgaagcaga cagctgaagc agggcacgag
    36721 attggcaatc acacatacga tcatcagaat ctggccactc tttctgccca gtcgatgacg
    36781 gaggaagtga cgaaagcaga cactgagatc aaaaaagtca ctggcaaaac ccccactttt
    36841 gttcgtccgc cctatggttc tgtcacgaat gtaggagcaa cgatcatcca gcgaccgatc
    36901 atcgaatggt ctgttgattc agaagattgg aagacaagaa atcctgattt gattttgcag
    36961 aagatccaag caacagtata cgatggggca attattttat tccatgatat ctatcctgaa
    37021 acgattcgtg ccgttcctca agtgatcgat tatctgaaag agcaagggta tcgcatcacg
    37081 acagttgggg atttattagg gcatccaacg gctgtcgaga actattatgg cagaaacgat
    37141 catcggccag ttcagtaagt gagacaagta tctttaataa ataacacatt ttaataagta
    37201 gttgaacaca taactacctt acttgcaaag gcttaacaat atctatacag gtacaacaaa
    37261 aaactgtcca ccaattttct ttggcggaca gttttcactt aaaaataaag aagcaactat
    37321 tcgtaaatta tgctaaaata gcagtagaac gcgtcctgag ggaatcgaac ccccgtctca
    37381 agaaccggaa tcttacgtga tatccactac actaaggacg caagcactgg gataattgta
    37441 actaaacaga taaaaaatta caaggggatt ggacaaaaaa atgaaatttg agcaaaattt
    37501 ttcacaaaaa cagcagcaga cacagaaact agccatgacg caacagttgc aacagtccat
    37561 ccaagtttta caatataaca gtgaggagct gttggcattc gttgagaatc aagcaatgga
    37621 aaatccattg gtggaagttg ttgagccgga atggcagcca gattacataa aagcttcttc
    37681 atcttcttat gaaggagaag agacaaatta cctaaatcag attcctgata caaaagggtc
    37741 tttatttgat tcattgatcg aacaagtcca tttgaattac cgagatactt ttttgaggaa
    37801 aatcgttctt tatcttgtag aatacatcga tttgaacgga tttttgacga ttagtttaga
    37861 agaagccgga aaagagattg gagctacgcc tatccaaatg ctcgatgcgt tgactctgat
    37921 tcagcaatta gaaccagctg gagtaggtgc acgtagtttg caagaatgtc tcatgcttca
    37981 aacagaacga gatgattatg cgccggaatt agcttatatc gtgttggaag aatgttttga
    38041 ggaactggtg gagcgaaaat ggaaagaaat cgcacaacgt ttttctgttg atctacatgc
    38101 tgttcagcag atattcgact atttacaaac gttgagtcca agccctggcc ggattttcga
    38161 ccgtagcagc gaattgtaca ttattcctga tgtccgggta ttagtggacg acgataaaaa
    38221 tgtacaagtt atctcaaacc gaaaaaatca gccaaatata cgctttcaag agagttactt
    38281 caaacaaatg tcacaacaag cagataaaga aactgaaaat tatttaaaag aacggaaaca
    38341 ggaattcgaa tggttgaaga aaacgatcct acaacgtggg gatacaattt tgcgggttgc
    38401 tcaagtcatt gtttcccggc aaaaagcttt ctttatcgac aaggaacgtc caatcaagcc
    38461 tcttacgtta aaggaagttg caactgagat agatgtgcat gaatccacgg tcagtcgtgc
    38521 agtcaacggg aaatatttgg aaacagactt cggtgtattt gaattaaaaa aatttttcac
    38581 tacacgaatt cctacgaata gtacagaaca gacagaagac ctttcagcag atacagcgaa
    38641 gaaaaagctg caggaattag tcgatcaaga agacaaaaac aagccgttgt ctgaccagaa
    38701 attggttgaa ttgctgaaaa aagatgagat agcgatctca agacgaacag tagcaaaata
    38761 tcgtgattta ttaggaatcc cgagttcatc aaaaagaaaa cgttatgata actaatcagt
    38821 caaaagacta gaaaaagtat ttcacttttt ctagtctttt tagttgaaaa acgagagcaa
    38881 aactgttaga cttacagtcg tgaggttgag atttcccttt attgatgtga aattcgaata
    38941 gttaatttct ttttttatcg tatatgggtc gttttaagtc tacattggac gtaaagtgtc
    39001 caactaagga gatcgctatg ctggatgaaa taaaaatgat tgaatctgtt gcaccagata
    39061 ttatcgaagt gttgcaagaa cgctataaaa ttttacgaaa tatctattgg atgcagccga
    39121 ttgggcgtcg aagtttatcg gaaagtatgg ggatcacaga acgagtctta cgaacagaaa
    39181 cagacctttt gaaaaatctg aatttgatcg acacctcaaa aagcggtatg acgctgacaa
    39241 ctaagggcga agaagtttat caaagccttg aaaacttcat ggatcagctt ttaggtacgc
    39301 atcaaaccga acaacagttg gcagaatatt ttggtaacca acgctgtatc gttgtttcag
    39361 gaaacagcga agagcagacc aaagtggctg atgcattcgg cgaagcgctg agcgaagcct
    39421 tagaccgact acttccagaa ggcgagaata tcatcgctgt gatgggagga actactatgg
    39481 ctgtggtagc cgaacaattg tctaatctgg aaaataagaa acggcataac ctgtttgtcc
    39541 ctgcaagagg cgggattggt gaagcgataa ctgtacaggc aaattctgtc agtgcaagaa
    39601 tggctgctaa agcaaacggt aaccaccgcg cactttatgt tccggaacaa ttaagtctgg
    39661 caacttacaa ttctttactc aatgagccat ctatccaaga agtcttaaac ttgattagtg
    39721 aagcgaattg tgttattcat agtattgggc gtgcattaca catggcagca cgtcgtaaga
    39781 tgactgaaaa agaattagtc atgctgaaac aagcaaatgc tgttgcggaa tcttttggct
    39841 actttttcaa tgaaaaaggt gaagtcgttt ataaggtccc tagaatcggc atagaattgc
    39901 aagatttgga aaaagtcccg atcatcatgg caatcgcggg cggaaaatca aaagctaaag
    39961 caattcgagc ttatatgaaa aatgcaccaa aacaaacgtg gctcatcact gatgaagctg
    40021 ctgcaaatga gattttaaaa ggggtaaccc tttaaaataa atattttttt gattttcata
    40081 aggaggaaat cttaaatgac agttaaagta ggtattaatg gttttggacg tattggacgt
    40141 ctagcattcc gtcgtatcca agatgtagat ggaatcgaag tagtagcaat caacgacttg
    40201 acagatgcta aaatgttggc acacttgtta aaatacgaca caactcaagg acgtttcaac
    40261 ggaacagttg aagttcatga aggttcattc aacgtaaacg gaaaagaagt taaagtatta
    40321 gctaaccgca acccagaaga attaccatgg ggcgaactag gcgtagatat cgttctagaa
    40381 tgtactggtt tcttcacttc taaatcagca gctgaaaaac atttaacagc tggtgctaaa
    40441 cgcgttgtta tctctgctcc tggcggaaac gatgttccaa caatcgttta taacacaaac
    40501 cacgaaacat taactggtaa agaaacagtt atctcaggtg cttcatgtac tactaactgt
    40561 ttagctccta tggctaaaac attgaacgac aaatttggtg ttgttgaagg attaatgaca
    40621 actatccacg cttacacagg tgaccaaatg actctagacg gacctcatcc taaaggtgac
    40681 ttccgccgtg cacgcgctgc tgctgcaaac atcgttccta actcaacagg tgctgctaaa
    40741 gctatcggtt tagtaattcc cgaattgaac ggtaaattag acggagctgc tcaacgtgtt
    40801 cctgtaccaa caggttcatt aacagaatta gtaacagttc ttgaaaaaga agtaactgtt
    40861 gacgaaatca atgcagcaat gaaagaagct tcaaacgaat cttacggata caacacagac
    40921 gaaatcgttt cttcagatat cgttggtatg acttacggtt cattatttga tgctacacaa
    40981 actaaagtaa tgacagttgg cgacaaacaa ttagttaaaa ctgttgcttg gtacgacaac
    41041 gaaatgtcat acactgcaca attagtacgt actttagaat acttcgctaa cttataagat
    41101 agacaaacgt acaagtgtaa attatagaca tgtacattta aaagcgggga agcaatcgcg
    41161 cttcttccgc tttttttgat aataagttta tt
//
LOCUS       DACDIH010000020        39825 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDIH010000020 DACDIH010000000
VERSION     DACDIH010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.020"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..889
                     /locus_tag="IV281_001098"
                     /pseudo
     CDS             1..889
                     /locus_tag="IV281_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
     gene            complement(985..3156)
                     /gene="copB"
                     /locus_tag="IV281_001099"
     CDS             complement(985..3156)
                     /gene="copB"
                     /locus_tag="IV281_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP8780128.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(3176..5362)
                     /locus_tag="IV281_001100"
     CDS             complement(3176..5362)
                     /locus_tag="IV281_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP8780129.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(5362..5571)
                     /gene="copZ"
                     /locus_tag="IV281_001101"
     CDS             complement(5362..5571)
                     /gene="copZ"
                     /locus_tag="IV281_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP8780130.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(5584..6024)
                     /locus_tag="IV281_001102"
     CDS             complement(5584..6024)
                     /locus_tag="IV281_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP8780131.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(6098..6637)
                     /locus_tag="IV281_001103"
     CDS             complement(6098..6637)
                     /locus_tag="IV281_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAP8780132.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(7252..8718)
                     /locus_tag="IV281_001104"
     CDS             complement(7252..8718)
                     /locus_tag="IV281_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8780133.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(9029..11110)
                     /gene="cadA"
                     /locus_tag="IV281_001105"
     CDS             complement(9029..11110)
                     /gene="cadA"
                     /locus_tag="IV281_001105"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8780134.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(11252..11431)
                     /locus_tag="IV281_001106"
                     /pseudo
     CDS             complement(11252..11431)
                     /locus_tag="IV281_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(11634..11993)
                     /locus_tag="IV281_001107"
     CDS             complement(11634..11993)
                     /locus_tag="IV281_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP8780135.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(12023..12364)
                     /locus_tag="IV281_001108"
     CDS             complement(12023..12364)
                     /locus_tag="IV281_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780136.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            complement(12361..13035)
                     /locus_tag="IV281_001109"
     CDS             complement(12361..13035)
                     /locus_tag="IV281_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8780137.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(14212..15510)
                     /gene="asnS"
                     /locus_tag="IV281_001110"
     CDS             complement(14212..15510)
                     /gene="asnS"
                     /locus_tag="IV281_001110"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP8780138.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(15550..16740)
                     /locus_tag="IV281_001111"
     CDS             complement(15550..16740)
                     /locus_tag="IV281_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8780139.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFLKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(16761..17288)
                     /locus_tag="IV281_001112"
     CDS             complement(16761..17288)
                     /locus_tag="IV281_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8780140.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(17335..20124)
                     /locus_tag="IV281_001113"
     CDS             complement(17335..20124)
                     /locus_tag="IV281_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8780141.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFIYVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMTTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(20271..20471)
                     /locus_tag="IV281_001114"
     CDS             complement(20271..20471)
                     /locus_tag="IV281_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8780142.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(20923..21243)
                     /locus_tag="IV281_001115"
     CDS             complement(20923..21243)
                     /locus_tag="IV281_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780143.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(21627..22745)
                     /locus_tag="IV281_001116"
     CDS             complement(21627..22745)
                     /locus_tag="IV281_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780144.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            23216..24523
                     /locus_tag="IV281_001117"
     CDS             23216..24523
                     /locus_tag="IV281_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP8780145.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            24642..25841
                     /locus_tag="IV281_001118"
     CDS             24642..25841
                     /locus_tag="IV281_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP8780146.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYAWQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(25868..27136)
                     /locus_tag="IV281_001119"
     CDS             complement(25868..27136)
                     /locus_tag="IV281_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP8780147.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(27299..27946)
                     /locus_tag="IV281_001120"
     CDS             complement(27299..27946)
                     /locus_tag="IV281_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP8780148.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALATEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMIYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(28354..28857)
                     /locus_tag="IV281_001121"
     CDS             complement(28354..28857)
                     /locus_tag="IV281_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8780149.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(28980..29744)
                     /locus_tag="IV281_001122"
     CDS             complement(28980..29744)
                     /locus_tag="IV281_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP8780150.1"
                     /translation="MKEIQSAKNPKIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            29852..30127
                     /locus_tag="IV281_001123"
     CDS             29852..30127
                     /locus_tag="IV281_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP8780151.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            30565..31515
                     /gene="yidC"
                     /locus_tag="IV281_001124"
     CDS             30565..31515
                     /gene="yidC"
                     /locus_tag="IV281_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8780152.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(31694..32668)
                     /gene="lacD"
                     /locus_tag="IV281_001125"
     CDS             complement(31694..32668)
                     /gene="lacD"
                     /locus_tag="IV281_001125"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8780153.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPECGLPAAALREKECGLLLAYEKTGYDTKMPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECDAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLKETATPVYVDR"
     gene            complement(32665..33123)
                     /locus_tag="IV281_001126"
     CDS             complement(32665..33123)
                     /locus_tag="IV281_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8780154.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIAHDEKEVYRK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIVTLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            complement(33120..34583)
                     /locus_tag="IV281_001127"
     CDS             complement(33120..34583)
                     /locus_tag="IV281_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8780155.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTILADNEAVDTLESETISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMLKTVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     SGGTGFLVGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVVFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPINKAAYVTGTALLAQGN
                     TTFMAGVSVACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEEGRAGGLS"
     gene            complement(34565..35515)
                     /gene="pfkB"
                     /locus_tag="IV281_001128"
     CDS             complement(34565..35515)
                     /gene="pfkB"
                     /locus_tag="IV281_001128"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP8780156.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQKKQIQTEFIEVPGMTRINVFTQVNQTREE
                     YKLVNQGPEIPQAAVHNFLNQIRALQAEDYLCVSGSLPRGMSPKVLVEISRICQEKEI
                     HLIIDSSDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENV
                     LLSLGEKGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLVGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            35658..36406
                     /locus_tag="IV281_001129"
                     /pseudo
     CDS             35658..36406
                     /locus_tag="IV281_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
     gene            36672..38150
                     /locus_tag="IV281_001130"
     CDS             36672..38150
                     /locus_tag="IV281_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP8780157.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRVESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
     gene            complement(38218..39309)
                     /locus_tag="IV281_001131"
     CDS             complement(38218..39309)
                     /locus_tag="IV281_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP8780158.1"
                     /translation="MLKKNTSLQNKTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFLFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
     gene            complement(39347..>39825)
                     /locus_tag="IV281_001132"
     CDS             complement(39347..>39825)
                     /locus_tag="IV281_001132"
                     /inference="COORDINATES: protein motif:HMM:NF012744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8780159.1"
                     /translation="KELLENLIKAYQLQNVIFLLGGQSNPYKYMSRADLYVMPSITEG
                     FPNALVEAMSTETAVLCADCKSGPREILSNRALDTICENIEYAESGILVCPMTDSRNY
                     SPQYEECDKNLANAIEELIENDNLRNKYAKAAKKRAELYSYDAFEHNLLQIMKTFN"
BASE COUNT        11630 a         8431 c         6901 g        12863 t
ORIGIN      
        1 ttgtcaggta tctttgacca ccgttattcg tacgttgaaa gagtttaaaa gctacttacc
       61 aaagcaatct aagaggattc tccccagagt attgatggtt gatgaatttc gttcgcacgc
      121 ttccatagaa gataaaatga gctttatttg cgcagatggc gaaacaggac aattaattga
      181 tgtcttgcct acgcgtaaat tgcctcgatt aacaagctat ttcttaggct gcgctaatcc
      241 tgaagaagta aaatttttag tcacagacat gaacgccgcc tacttccagc ttaccaaacg
      301 tattctgcca aatgcgaaag tagtgattga tcggtttcat attatcaaac acatgaatca
      361 agcctttaat gagttgcgta tccgtgaaat gaatgaactt cgtaaagtag gacagaaaag
      421 tcaggcagaa aaactgaaaa agaactggcg ctttttgcta aaaaatcgtg caaacatcaa
      481 ccattatgaa tacaaaacat ggaaaagttt tcgagcacca aaatacccat ttcttacgga
      541 agcgatgatg attgatcgat tgcttggctt ttctacgtcc ctgaaggagg cgtatcccta
      601 ttttcatgaa ttagttgaag cctttcgaga caaagaccct gacttatttt tctccttatt
      661 ggcagaactt cccgaaacgt tagatgacgg ctttcgggaa aagctccaaa accttctgac
      721 ctatgaagaa ggcatcacca acgcgatgat ctatccttac accaatggaa aaatagaagc
      781 gaagaatacc catataaaga caatgaaatg agtatcctac ggatttaaat catttgagaa
      841 catgagaatt agaatctttt tgatcaatca attaatcaaa gtaagataac aaaaaatctg
      901 agccagaatg aggttcattc tgacccagat ttgattttgt acaacccatc agtccttatt
      961 gacaaagagc cccttttttc attattattt gattttcaat gtcaaagcat ttagagctac
     1021 aactaccgta cttaacgaca tcaagacagc accaactgct ggacttaata tgataccaat
     1081 tggcgcaaga attccggctg ctagcggaat tgcaagaata ttgtaacctg ccccccacca
     1141 gaggttctgg atcattttct ttcgcgtttg cttggctaga tcaagaaaat gcaaaatatc
     1201 ttttggatcg ctgtttgtca atacgacatc cgcagaatcg attgcgatat ctgtccctgc
     1261 accgattgcc atcccgattg aagcacgggc taaacttggc gcatcattga tcccatcacc
     1321 caccatgatt acttttttcc cttggtcaag gtatttctgg acaacagctt ctttatcatc
     1381 tggtaataaa ccgccataat attcttcgat ccctaaataa tctgctactg cttgggcagc
     1441 gtttttattg tcaccagtaa gcatgacagg agtgattttt ctttctttca atgttcggat
     1501 aaattctttt gcttctgttt ttactacgtc acctaaagca acgattgcag ctagatgatt
     1561 ttctaaaatc aggaagctga tggtatttcc ttgttcttgg taaggtttca ataatgcttg
     1621 ttctacgttt aatccgagac gctcaacttc tttttcatta actattttca cttgttgttt
     1681 tttgactgtt gcctctagcc cgactcctga aagatttttt agattttcag cttgataagg
     1741 cttgatttca tgttctttca aataattcat aatgccaaca gccaatggat gattggcatt
     1801 cgcttccaag gctcctagat attgaaggat ttcattttta ttgaacgcat catcaagtac
     1861 ttcgacaccc gttacagtaa attttccttc tgttagtgtt cctgttttgt ccagcatgat
     1921 cacatctaga tcattggctt gctccagtgc attgcggttt ttcaaaagta atccgttttt
     1981 agctgctatt gatgtggagc gagcaacaac taatgggata gctaaaccta gagcatgagg
     2041 acaggcgatg ataaagactg ttaccatacg ttctaatgca ttaggaagat ctgtcaagaa
     2101 caaccaggcg atgaatgcca ggatacccac aattaatgcg acataaaaca gccattttgc
     2161 cactttgtca gataatgact ctagcttgga tttttctcct tgtgcttttc gcaccatttc
     2221 cataaccttc gctagataac cattttcacc agttccagtt acttcgattt cgatcgttcc
     2281 atccccattg atagaaccgc caatcacttt gtcattggct tgttttttga ttcctcttga
     2341 ttcgccagtc acagctgatt cgtcaacaat tgtttcacct ttcaagatta caccatctgt
     2401 tggcatttta tcaccagacc ggacgatcaa acgatcacct tcgtttactt ctttcaatga
     2461 aactgtctct tcttttccat ctttcgtcaa gcgtttcact gattctggca gtaattctgc
     2521 tagtttttgt aaagcattgc tggcattcga cacagcattc atttcaatcc agtgacctag
     2581 tagcataata acgatcaaag tagccaattc ccagaagaaa tccatgacgt gtgtatgtgg
     2641 attgagcaga tttgcgataa acgaatagac actataaata tatgcaactg taatccccat
     2701 cgcgatcagt gtcatcatag caggactttt ttgtttcaat tccatcttag ctccacttaa
     2761 aaatggctga ccgccataga taaatagtat agttgctaag atcaatacga gccaatcaga
     2821 accaggaaac gttacttgaa atggaaattc cattcccatc attggtgaaa aaagaatgat
     2881 tgggatagct aggataagcg atagccaaaa tttttgtttg aaattcccca tgtgcatgga
     2941 atgatccatg ccgccgtgtt cgtgatccat gtgcatcgaa tggtccattt gtttgctgtt
     3001 tgctatatct ccatggttat gtcccatgtg catggaatga tccatctgac tatggtcatg
     3061 tttcatctcc atctgttttt catgagattc tgtactttct ttgtttacta cttctttttt
     3121 atgctcatgt tttttctcca tatcgttccc attcattttt actccatccc ttctcttact
     3181 ttttcatgtg tcgatttaag ctcagggagt tcaacaatac actgacggaa ctaaacgcca
     3241 tcgctcctcc tgcaatgatt ggattcaaaa agcctagtgc ggcaaaagga ataccaatcg
     3301 tattatagac aaaagcccag aaaagatttt gtttgatttt tctcaatgtc aacctagaca
     3361 actcgattgt ttgtgcaatg gatgccaaac tgctattcat cagcgtcaca tcggctgttt
     3421 ccatcgcaat gtccgttcca cttcccattg ccataccgac atcagctaat gctaaggccg
     3481 gtgcatcatt gattccatcg cctgccatac cgactttttt gccatctttc tgaagttttt
     3541 ctacgtaggc tgctttatcc tctggaagta cttcagcaaa gatatgcttt ggatcaatac
     3601 caacttgttt accaatcgtt tcggctgcca gtttgttgtc tcctgtcagc ataaaaacat
     3661 ctaatccttt attctggagc tgcttgattg cttgtttggc ttctagtttg atttgatcag
     3721 ccacggcaat ccacccgatc acttgctttt catcagctaa aaacatgact gtttttccct
     3781 gctgttctaa ttctaacgcg tgttcttggt attcatcaaa tgataggttc aattcaatta
     3841 atcgtttacg tgtacccgca aaatagcgta ctccattaat cgtaccgctg atcccagcac
     3901 ctgggtgggc tgtaaaatca atcatatcgt atgttgctgc ttcttgcagt tttccgtatt
     3961 ccacgattgc tttacctagt ggatgttcag aagcttgttc taaagtataa aaaatagata
     4021 ggacttcttt tgaaccgact agatctgtaa ctttgggctt tccttctgta atcgttccag
     4081 ttttatccaa aacaatgctg tctaagtgag cagctgcttc taatgcttct ccgcctttaa
     4141 ttaaaattcc atttcgtgca ccaacacccg ttcctaccat aatagctgtt ggggtcgcta
     4201 aacctaatgc acatggacac gcaatgacta atacagagac actatggatc aatgcgagtt
     4261 gccaatcacc agtgatcaaa cctgtagcta tgagcgtgat aaacgcgatg actaatacga
     4321 tcggaacaaa aatccctgag atccgatctg cgattttttg gattggggct ttgctccctt
     4381 gagcatcttc caccatttga atgatttgcg ctaatactgt ctgcttgccg acttgtgtga
     4441 cttccatgtg tagcagacca ttcgtattga ttgtcccgcc aaaaagcgtg tcatccggat
     4501 tcttttctac aggaagattt tcaccagtaa gcatactttc atctatcgca ctgcttcctg
     4561 aaataattcg accatctgct ggaatctgct caccaggacg gatgactagt tgatcaccaa
     4621 cgactacttc ttcaatcgct aacgtttctt ctttcccgtt tcttatcact tgggcggttt
     4681 ttgtttgaag agacatcaat tgtttgattg cattacctgt tttcgttttt gctgtatgtt
     4741 ccaaatattt gccgagcaaa atcagcgtga tgatcatact gctgctttca aaataaagat
     4801 cttgggggtg tccacggaaa aatccgttgt aaatgctcaa tgcaaaggca gccgatgttc
     4861 caattgctac caatacatcc atatttggtg ctttcgtctt gattgcatgg tacgcacctt
     4921 tataaaaacg agcaccgaca taaaattgga caggagcaga cagcactaac tggacgattg
     4981 gaaaatgaaa aaagtgaaca atcgccgcat ggcttcctaa catcatagca atcattgaca
     5041 acatcaacgg caaagtcaaa accgtactca atataagatc gaatagcatt tttttcaaat
     5101 acgcttgctt ttcttctgca attttttgtt tgtgggcttc atcgtacaaa attgcaccat
     5161 aaccgatgtt ttctacgctt tggattaatt tttcagtggt cgtcccttca tatttgacac
     5221 ttgctttttc agtggccaag ttgactgtgg cattgaccac accaggttgt ccgttcagct
     5281 ctttttcgat cctagcagaa caatttgcac aagtcatacc tgtaatgacg aacgtatcca
     5341 ttttggtttc tgctgttgcc atcaaatcac ctctgcttta taacccaatc cattgattgc
     5401 ttgacaaatc ttttcggatt gaacttttgt ttcatcaaac ttgaccgtac cgtttgcttt
     5461 tttcaaattt actttcactt tttgcacgcc gtcaagtgcg cttactgttt cttctactct
     5521 tgctgcacaa tgattgcagc tcataccttc gattgaaaat tgttgtttca ttttttcttc
     5581 ctcctattga tgttgtttac attcacattg acctgggata cagttacatt cgatcgtatc
     5641 tactggttgc ttttgttcca gttgtttcaa aatctcattg atatcttctt gagtcaaagt
     5701 ggcttcttct actagatccg caatcgtctc tccaatcctt tttgcacaga tatgggaaaa
     5761 aagattttcc gtcgcactgc gaactgtttc catctccgag acagctggat gataaatgaa
     5821 tttttttcct tcttgttctg tccagatcac ttcttttttt accaaacgtc ccagcaaagt
     5881 tttgatcgtt gctggtttcc aatccatcga atcagctaag atctgcgtga tttcttgggc
     5941 tgtcgtctgt ccaagtgtcc aaataacacg catgatctcc cactctgaat cactgatctt
     6001 tactggcgtt ttgacttttt ccatttattt cacacctttc gtttacaaat gtaatcatta
     6061 gcttatgaat acagattacg cttgtaaaca aaaactgtca attatttttg ctctctattt
     6121 tcaatttagt taaaaattct tgtttctctc gtcttgtttt aaggatgatc agtttatttc
     6181 ttgcattagc tacttggatc agctgctgta ttttcggttc attgttttct tcaaaatgcc
     6241 agataaaaga taaaaattct aagtattccc tgtttagttt ctctgaaaat gattcaggca
     6301 tatctggccg agattttttg tttatctttc tttttagaga acgccaaact actcggtaga
     6361 tggcgagata acgcggcact ttaagccaaa tgatcttatc tgcttcttta atcctttcct
     6421 ctaatgtaga agtataattt ccttcaacaa tccatgacgg attagatacc ataaacgatt
     6481 gctgttcttg caaaaaccac ttttttgcct gaatactgta gtctgttgta tgccataaaa
     6541 ggtctagcgg cagtagcgga aagtcggtga tttctttgat cttccttgcg agggtcgatt
     6601 ttcccgaccc actttgtcca atcacgatga tcttcattct tctccctcac ttctctttgg
     6661 aatttggttc gatcatacca cttataacgc cgcaaaaaaa caagggactg tgacaaaagt
     6721 cgtgtcaaag tcccttgttt cgaataaatg gtgttcaatc agctgacctt cagcattaac
     6781 tcaaaaaacg gcaaaatatg aggagctatt ttttcgtttg ttcctctaat actcaggtca
     6841 aatcgctgat tttacaacct cttccttaaa cggtgttctc ctgccaacac ctcggtcaca
     6901 aggcacaata gtcaacaaac atgagaagtt gttttttgaa attgctcctt gtgactcgga
     6961 gttaaacggc aggctcacta cctctttctt aaacggtgtt caaaagcttg catctgcggt
     7021 aataagccca gcaaaacgaa aaatatgagg ggctattttt gtttggctgt tcttattact
     7081 tgatgcagga cagctttttc acaacctctg attcacggtg ttccagaagt agcttctgcg
     7141 aaaaacaagc cgaaacttca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt
     7201 tcttgaagct gaccacttct gtcacaacct cttgtaacaa gtatttctat tttaaggctg
     7261 aaccactgtt tctggttcag tgatttcttt gtttttgtct cgacgagcaa ttgctggcaa
     7321 gatcatacct aataagatca agacaattgg tgtgatgata ttcgtgaata attggaacca
     7381 ccatgcttta ggatcagcag tataatctac ttttggcacc attcctagga tacatgcaaa
     7441 cgcagtgaag gcaaagcacc aagcaccaaa gataaaaccg atttttggat ttttaacaaa
     7501 tttatattca gaattgaatt tcttataagc tttattcaac atcatatatg ccaagaacac
     7561 ccataggtaa cgcattggca tgacaactga gttcaagttc gtcaaccatt ttactaattc
     7621 attcatatct ttgataccaa agatcggcaa caaaataatg attcctacta gtatacctgt
     7681 cagaagataa ccattcacca atgtaccttt acgattacgt ttacgtagcc agtttggtac
     7741 aaattctgga tccgcatctg ctaacaagat ttttagtggt gcatcaattg aaaaggctaa
     7801 tgcggagatt tgtccaatca tgtttgtcaa tgcgtaaatg atcatcaatg agttaccgat
     7861 accatagtaa ttacctaaaa tttgaaaagc actgtatgcc ccatttgcca tcaagtcttt
     7921 aggaatatta tcgctagcaa acagcatacc cattgcgact gagcctaata cagcacaaat
     7981 ccctaccatt gctgctaaga agatcatccc tttaggaaat tcctttgcag gatttcttgt
     8041 agagttgaca taaggggata ttttttccgc tcctccaact gcaaatacta gcatcgaaac
     8101 ggttgtgaaa taactgaaat caaatgttgg aatatatgtt ttcacttgat tcatatgtgg
     8161 tgtggcaaat tccatcgttg gattgatgaa cggtgccgtt acagctaaaa gaataaataa
     8221 gatcgacatg acgaacatcg ctgttccggc cagtccgcca atcactttca aagtagaaag
     8281 tccttttgtt gataaatata aaaaggcaag aaatataata agtgaaataa ctgctaccat
     8341 ttgtactgac atcgtattta cgatatttcc atttccttgg actgcccaac cgccagcaat
     8401 caggattgct tgaggctttt gtgccaaata aggaatatgg acgacccagt aagtccaagc
     8461 ggcatagtat gccagtcgtt tattagatgt cgtattttcg acccatgaac tgacgcctcc
     8521 tttactatcc ttgaaggctg accctaattg tccaacgatc aatgcgtatg gaataaaata
     8581 aatggccaaa atcaatagcc aagaggtgac aactgatact ccttgctgag catagttatt
     8641 aactacattc cccatgcccc aaaccatgtt aaatgcaatt aacgcaacag taaaccagcg
     8701 taattgtttg ttgttcattc gttctctcct ttatgataaa aatttgttat ccagatgtaa
     8761 tcatattcaa tttttaacaa acttacaaga cagtgttatt ttatgtgtgt attcttttat
     8821 ttaatacaca tcgttttttc atattttttc catcaaaaaa ataagttatt ttcttttatt
     8881 tcatttacct tattgaattt caagctattt tgaattttct gcaaaattaa aaacattagt
     8941 tattttataa tcctatctat tttctttaca tttttcatta agaaaaggat ataaaaaaaa
     9001 gaggacgttc attcgtcctc ttcattgatc aacgtttatt ttttacatgc aagcttcgca
     9061 tggcattcaa aacagctaat actgtcaccc caacatctgc aaatactgca tctcccattg
     9121 atgcaaaacc aaaagcacct aaggcaagta ccaaaatttt tatcccaata gcaaagacaa
     9181 tgttctgttt gacgattttc aatgttttct ttgctaaatg gattgcttct gcaatcttcg
     9241 atggttgatc attcatgatg actacatctg ctgcttcaat tgcagcatcg cttcccaatc
     9301 cacccattgc gattcctaca tctgctcttg ctagcaccgg tgcatcgttg attccatctc
     9361 cgacaaatgc ggtcttttta ttgtttcttt gtaagatttc ctctaaccgc tgcactttat
     9421 cttctggaag caattcgcta tatatcttgt ccacgcctac ttgcttcccg atatgatcag
     9481 caattttttt cgaatcgccc gtcaacatga ctgtgttctt cacaccagcc ttttttaatt
     9541 gaaccaatgc gtctacggca tcttctttca aggtatctgc aataacaaga taaccactgt
     9601 aactttgatc gatcgctaga taaagcaatg taccgatttc ttgacttgaa ggaaaagaga
     9661 tttcaaatct ttccatcaat ttgtgattac ctgctaatac ttgtttgcca tcaacttcag
     9721 ctaagatccc ttcacctgca aattcttgga tattagctgt tggtaaaagc ggacctgcgt
     9781 agccttctaa aacagattga gcaatcggat gtgtagaaaa ctgttctgca ctcgcaacat
     9841 attgcatgaa tatctttgga tcgatttttg tatcgatcgt ctggatcgaa aagtcacctt
     9901 tcgttaatgt gccggtttta tcgaatacca ctgtctcagt ttgtgcaagc aactccaagt
     9961 aatttgaacc ttttactaat acaccgattt tacttgctcc tccgataccg ccaaagaaac
    10021 taaggggtac ggagatcact aatgcacatg gacatgagat cactaaaaat gtcaatgctc
    10081 gatagatcca ttcagaaaac gccgctccag aaacaacaag tggaggtaaa acagctaata
    10141 aaacggccaa tcctacaacg actggtgtgt aatagcgcgc gaatttagta atgaaatttt
    10201 cagctggtgc ttttttactg ctggcatttt ctactaattc taataatttg ctgatagtag
    10261 aattttcata agatttttct acttgaacta aaaatggttt atttttattg ataaatccac
    10321 ttaatactgt ttcaccaaca tatacagatt tagggactga ttctcctgta agtgctgacg
    10381 tatcgactaa tgattctcct tcgatgatcg ttccatctaa cggtacacgt tcaccaggct
    10441 tcacaagtac aaactgaccg atctgcacgt cttcagggtt cacggtcatc aatccttccg
    10501 tcgtctgcac gtgagctgtt tctggacgga tcgccatcaa ttcgcgaatc gattttcttg
    10561 aatgactgac tgccaatcct tggaaatact cgccgatttg ataaaatagc atcactgcta
    10621 cggcttctgg atattcaccg atcaacatag cgccaatcgt agcaacagac ataagaaaat
    10681 tctcatcaaa gatttttcct ttgacgatat tcatcactgc cttcttcact acatcatatc
    10741 ctatcagcag ataaatcaat aaatacccta tcaagcgaat tggttcatta aaatcaaata
    10801 atagcaataa agcgatacct gcaagggaaa ataaaatccg gaacagcgta tttctctctt
    10861 tttcatttcc atgttcagat actttttcct tatctatttc tgttactttg acatctggtt
    10921 ctaattcctt cactactttt tttatttctt gttcaatcgt agccaaatga tcttcctctg
    10981 cgtcaatcgt tagttttcca gaagtgaagt ttaccgttgc ttccttgact ccattgatct
    11041 tctgaactcc tttttcgatt ttcattgcac agttcgcaca atctaaacct tctaatctgt
    11101 aacttttcat tttaatgacc tcctcgcctc aacatatgaa tatctcttca taactaatta
    11161 taaatgaacg gttattcata tgtcaaggta tccgaaagaa aaaatgaaaa acatctgcat
    11221 cggaaaattt cttccataca gatgttttgg cttattcttt ctttttcttt tcgatcattg
    11281 ccactgctct tatccaaccc gcacttgcct tcttgatctt gacaggatag caactaagtt
    11341 tgaagcctgt tgctggcaat ttgtctaaat tcattaattt ttcgatagga caatatcttt
    11401 tttcaatccc tgcaaaatgt ccttcccaga tattcgaagc tccactacgc tttcaaaaat
    11461 atagaaagag ttctttaatg aaaattattt caactttcca taaacagtaa ccccatataa
    11521 ataaaagacg taaaagtcac atggaagaac ttatacagga gaacgcatag agtcaggtga
    11581 aatacatgta tcccacctga ctctatgctt tatctaacct ttattattct cttttataca
    11641 atttctgcac cgaatggcat caacgccaat cgagcaagtt tgaaagattg cttggcaaaa
    11701 ggtatcccta tgatcgtgat acatagaagg atgccgctga cgacatgtgc gagagcaatt
    11761 ggtaggcctg aaacgatcag ccagatgata ttaacaatca atgacatgcc agactggtga
    11821 taaattactt ctttgccaaa aggcgcaaaa gaaagggagg cgatcttaaa acattgcata
    11881 ccgattggga tacctatgat cgtgatacac caaagtatcc ccgctgctaa ccagctaaga
    11941 ccaccgatta gtccgccgaa tataaaccag atgacatttc ctaaacaact cattgtattt
    12001 ctccttctat tactgttttt gattaaaact gggcaaaaaa tgattttatt cttctttttt
    12061 tagcttccag tatcaaatat tctgcaaatt ttttttggat ttcttccgta ttttcttttg
    12121 ttactaatgt gtgttggatt agttttcctg atggtcgctg atccaatcgt tcttctgact
    12181 gttgcacgtc cgcttgcgca aatccgataa tccgtttttc ttttgttaca gcaacgagca
    12241 atagtttatt cttatcattt aggtcagctg ccgtttttgc ttttacgaat gattctggat
    12301 gatcagtaat cagctgattg atttgaggta gatcttcttt tactgcccac cgtatttcca
    12361 tcatttctca cttttctttc gctcattttt atctccacta aaaaaatcga ctgcggcaat
    12421 tgcatctatc cagctttgtg caatgatctc atgtccgcca atcgttgggt ggaccccatc
    12481 ttctcttgta tatttcgaaa aaccataaac aatccctaaa gcattgaata taccatctaa
    12541 aggaaccagc aatgcgccat attccttagc aagtttacga acaatttgga ttttagggtc
    12601 taagtcttta cgccagccat gacggtcgtc ggttatagga agtacaaacg gctccatcaa
    12661 gatgatgttg cagattccca ctgtttgaag ttcatttagt aattttcgat aaacggattc
    12721 aaattgctga gcagatttct cagttccgaa tatagcctga tcgctagtat tatgccaggt
    12781 atcattgatt ccgatcaaaa tcgatacata gtcaggttgt aaagctaaac aatcttcttg
    12841 ccagcgtact tgcagatctg caattttgtc tcctcctatc ccacggttca tgcattcaaa
    12901 ttccatcgtt ggatattggg agattagttt tccggcaatc aaaagtggat aacctttacc
    12961 tagatcagtt gcttctgctc ggtctcttcc tgcgtctgta atgctatcac cgataaataa
    13021 tatacgtttt ttcatttgtc aaaaactcct ttttgaataa aacgatgatt ttcttcctta
    13081 tattatccca taagccctat caagacgaaa gagaaaatat atgtataaaa aatgacacca
    13141 gcctagtccc ttcaaacaat tgaatgaacg ctcatcaagt gtctagtttt ttatgctatt
    13201 tttttcttaa cgtgccggat tccccttgat tccttcgttg tttggtccag agagatcgat
    13261 cacacctaag cttccgtcaa tcagaccact gatgacgcca tcagaacata tatttatagg
    13321 ctcatctaag acttctagta aatgtccctt tactaatatc tcttctagat tgcttgggat
    13381 agattggtca taactttatc tggtccgatg atttcaaggg tgaattgaac ttacatatgg
    13441 attattgcat gctgaaaata cggacctact ccatcttcga atgttactat tagtttgttt
    13501 tcaggtgcta gatgctgctt tatttcaaca gcagaagcat catcaaatca gatttccatt
    13561 tgaattacct tctttgtaaa tgtttgattg attgcgattt cgttatagaa ccttgtgaat
    13621 caaatagcaa tttcttttgc tttcttctct tgtctgctgg aaacttcctt tatcccgtat
    13681 tttaaaaaaa gaactgcgag agttcgcagt tcttttttag aataaacggt gctctccggc
    13741 caactcctcg gtcacaagtc acaatagtca tcaaacatga aaaactgttt tttgaaatta
    13801 ctccttgtga ctcggagctg aacggcaggc gcacaacctc ttgatataaa cggtgttcaa
    13861 tcagctgacc ctcgatatta gctaaaaaaa cggtaaaata tgagaagcta tttttccgtt
    13921 ttgtttctct aatactcagg tcaaggcact gatttcacaa cctcttcctt aaacggtgtt
    13981 caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtggaaa gccatttcca
    14041 caaaattttt cttaatactc gaaactaaac agctttttca caacctctcc actaaacggt
    14101 attcaaaagc ttacatctgc ggtaataagt cccgccaatt caaaaacatg aacagctgtt
    14161 tttgcttggc tgttctaatt acttgatgca gaacagcttt ttcatcacct cttatggata
    14221 aattcggttc aacaaacgtg ggaatggaat agcttcacgc acatggtcga taccagcaat
    14281 ccaagtgact gttcgttcaa ggcctagacc aaaaccagag tgcggaacag aaccgtattt
    14341 acgtagatca agataccatg aatattcttt ttcatccaaa ccgtgattac ggatttgttc
    14401 tagcaagaaa tcatagtccg ttgcccgttc gcttccgcca ataatttcac cgtaaccttc
    14461 tggtgcgatc atatctgcac aaattaccac gtcttctctt gttggatgtg gtttcatgta
    14521 gaatggcttg atagcttttg gatagtttag gataaagaca ggctgttcga atgaattcgc
    14581 aatgaacgtt tcatgaggtg aaccaaagtc ttcgccccat tcgatgtcat caaatccatt
    14641 tttcttcagt aattcgatcg catcatcata agtaatacga gggaatggca attttgtata
    14701 agtttttaag acttctttgt ctcgtcctag tacatctaat gcgtattcgc aatgatctaa
    14761 aacattttgt actaagaatg ctacatattg ttcctgtact tctaaacttt cttcttgatg
    14821 agtgaaagcc atttctggtt cgatcatcca gaattcaatc aaatgacgtc gtgtttttga
    14881 tttttcagca cggaaagtcg gaccgaatga aaagactttt ccgaaagcca tagcagcagc
    14941 ttccatgtat aattggccac tttgagataa ataagctttt tgatcaaagt aatttgtttc
    15001 gaatagatcc gtagtgcctt ctggtgcgga accagtcaag atcggcggat cgattttgac
    15061 aaaatgatgt tgattaaaga attcatacgt tgcacgaatg atttcatttc tgatctgcat
    15121 aatcgcatgt tgtcttgaag aacgtagcca taaatgacga tgatccatca agaagtctgt
    15181 tccgtgttct tttggtgtga tcggataatc gtggctctcg cttaccactt ccacatttgt
    15241 aattccgatt tcataaccaa attttgaacg gctgtcttca cggatttctc cagttactaa
    15301 cacggatgtt tcttggtgca gatttttagc tgtttggaaa acttcttccg gcacttcgct
    15361 ttttacaact acaccttgga aaaaggctgt tccgtctctt aattgtaaaa atgcgatttt
    15421 tccgcttgat cgcttatttg cgacccaagc gccgattgtt actgtttctc ccacatggtt
    15481 tttcgaatcg atgatttgaa tttgttccac gtatgactct ccttttatat tccttgaatt
    15541 tcttcaggat taattttgac ttttactctc aataaattgc ttgattctgc gtaccgcttc
    15601 ttctaacgta gcgcgatcag tggcataact catgcggaca ttttctggtg ccccaaagcc
    15661 ttcgccagtc acaagcgcta cccctgtttc ttctaaaaga tcttctaccc attgtgtgac
    15721 attttcatat ttacacatgc gtagcgtttc ttttacatta ggaaaaagat aaaaagctcc
    15781 ttgtggcttt tcaagagaaa cacccggcag ttctgccaca agcggatata acgtattcag
    15841 gcgttcttca aaggcgttcc gcatttcttc cactgtatct tgctcgcctt tcaatgcttc
    15901 aacagctgca tattgactga ctgctgtcgg attgctagtt gattgggaag caacagcgat
    15961 cattccattg atgatcacag gatttcccac cgcataacca atgcgccagc ctgtcatcgc
    16021 atatgtttta gacacgccgt tgatgatgat ggtctgattt ttgattgctt ctgaaatcgt
    16081 tgcaattgga gtaaactcat tgccattata aactaaacgg ccataaatat catcagcaac
    16141 tatcaaaata tcatttttta ctgcccattc accgattgcc tgcagttcat tcttgctata
    16201 aatcattcca gtcggattcg atggagaatt gatgattacg gcttttgttt tttctgaacg
    16261 tgcttgttct agctgctcaa cagtaacttt ccagctattg gattcttctc ctttcaaaaa
    16321 gacaggaagt ccttctgcca gttttacttg ctcaccgtaa ctgacccagt aaggcacagg
    16381 aatgatgact tcatcttgcg ggtcaaggat cgtttggaaa agggtataca aagcaaattt
    16441 cgctccgtct gttacgactg tttgtgaagg ttcatagaca agtccatagt ttttcttcag
    16501 ataatcaacg attgcctgtc gaagctctgg gattcctgct gttggtgtat aatagctagc
    16561 tttcccattg cggattgctt cgattgctgc ttcttggatg ttttctggtg tcgcaaaatc
    16621 cggttctccc acagtcaaac tcagcacatc tctgcctttg gcttttaatg ccttggcctt
    16681 tgccgcagct gccaaagtga cagacggttc taatcgtttt gttctttttg ataactccat
    16741 cccattcatt cctttacctt ttaaattccc ttgattgtct tgacttcatc tccagattca
    16801 aagccaatca ggtaataatt cagtttccca tttgtttctt ttcctactac ttcccacaca
    16861 ggctgatcat catacatccc taaagcagct ttttcgattt ggatttcagg atgggccgca
    16921 gcaattgtct gttttgcttc attttcagtc agtccctttg tttgatccaa tacttttact
    16981 ttttcaccag atttaggaat gataacggcg atttcttttc ctttatcatt tgtacctgtc
    17041 agtgaaaagt atgtcttctt tcgagtaaac caataaaaat gatcggcttc ctcaagattt
    17101 gcatatttct ttgcgatttc tactgcttct ttcttcgcct gcaccgccgg acgactggaa
    17161 cgcaagtaaa aaatcgagct gatcacgatg accgctaata aaaatacgat caagccaagt
    17221 aacacgcgat taatcgtttt ttcctttgtc tgttttttct tcaagggtcg tcgctccttt
    17281 atcttcattc cttttcatta tagcagattt taattatgaa aaaagacagc tcctctaact
    17341 tccttcttca tttttcttta agaattcatg gatatcctga tagatttcct ccatagctag
    17401 ctctttgatc ggtacttttt tcggtaatgc atggatgatc cgtgcaccat attttgcagt
    17461 aactagtctt cgatccaaaa taagcaatac gccacgatct gtctccgtcc ttatcaagcg
    17521 tcctaatgct tggcgtaact tcaaagctgc tttaggtacg gattcctggt aaaaaggatt
    17581 gatcccctct gattctaaat aagcgttcct agctttgacc atcgggcgaa gcggattatc
    17641 gaagggtaaa cgtgtgacta caacgatctg caaagcatct ccaggcaaat ctacaccttc
    17701 ccaaaaactg tcagcgccaa acaatacgct tttttctgac aaaatgaagc gtttcaaaag
    17761 cttctcccgg cttccgccaa acccttgagc tagtatttct cgtccttttt ctagcataga
    17821 cagatgcatt ttctgataga ccttctgcaa aacatcatga gaagtgaata acacaagtat
    17881 tggccgatct tcttgttcga tcagacctgt aagtgctgtc tctaaataat cgatatatgc
    17941 ttgattgctg acttgatgga cggcaggtgc ctcttttggc acatagatcc ttgcctgttt
    18001 ctcataatca taaggagagg caatcacttt aagaggtgct gcttcaatgc caagccgctt
    18061 agagagatag tgtcgattag ggccgatctt cagcgttcct ccaatataaa gaatacgatc
    18121 atatcgagga taccaaacag tcgacggcaa catagccgct tgaaaatcat ggagatgaaa
    18181 aatcatctgc tgcgctgtgt tcccaggaac gaaccaatgg atcaaatgat ctcgccagtc
    18241 ttccagccat tgatccatca gcaagtactg cttgtctaat tgatcaaata agctatataa
    18301 acttgctaaa tcgatccgca tctggtttgt ccagctttct tggataatct caaaagtcag
    18361 acgcatttgt gactgtagct gctggatctc ctgataaaaa agctgcaggt gatcaatggc
    18421 tgtcccagat tccagtggca gctgatccaa catctctttt gtcgtcatga cttcttctgg
    18481 atactgtccg ttaggttgct tgccgctcac taaactcatt aatccttctg ccatacaaat
    18541 ctgtgcttcg catagcgcag aaagttcttc tcgataaatc gataaccagc gtaaggcttc
    18601 atgttctttc ggtaaatagc tttgtattcg gtcaaacagc tgatttggtt cttctaattg
    18661 ctggatttgt ttatggaaat agttaatact taatcgtttg tcatttactt tttcagcgat
    18721 ttccggcaaa tgatgtactt catcgatcaa caaaaatgga ctagctggaa gcaatggttc
    18781 ttttcgctgt gtttcttgta ttaaaaacgc atgattgaca atcaaaaaat tactttgcct
    18841 gattttcttt tgcaagaaac gaacaaagtc ttgttcgtaa aatgactgat ttcttgctaa
    18901 aaatccagtg cctctgtgcg cgatatctgc aaagaatgaa tggttcaaat ttgttaaatg
    18961 taattcatcc aaatcaccgg tcttcgtttg cgtcagccaa actaagaccg ccatttgata
    19021 caaagcatat tgtttttgtt ctggcggttc cacaagtgtg cctttaaaac gctgtaaatc
    19081 aatatagtgg cgatagcttt tgataattac aggttgtatg ggctgatcta gcaattgatt
    19141 cagcaatgga atgtcttttt ctatgatctg ctgctgcaaa actagcgaaa cggtactgat
    19201 gacagcgggt ttttcaggtg tagccaaata actcaatggc agcagataac cgattgtttt
    19261 acccattcct gtagctgctt cgatcatcaa atcttttgat tcatcttttg tgaaatggtc
    19321 gtagaccgca ttcatcagtc tgttctgttc ttttcgatac atcagtgttt taccaaacat
    19381 tttttctttt gcttgttttt ttcttggata tgtccgctcg ccataatgga cgtatatgaa
    19441 tagttccact tcttttttct gcaaagctag cccatcgacg atttctaatg agggatcaag
    19501 tggctctggt ttttctttca tttcttcaat cacatgataa atgaaacgac tcgtatccat
    19561 acctgtgtgt ctacttaatc gcgcaatctg ctctaacgtg atgattggca attttctcat
    19621 ccgttcttcg atcagcaaaa gaagttgacc cgtcacttcg gcatcactat ctgcctgatg
    19681 aggattctca tgaataaatc caaggctttc tgacaaatca gctaagcgaa agcttggttc
    19741 cgtcggcaag aaaatttgtg caagttctac tgtatcgatg cctggtatct tcaaactagg
    19801 aagtccgcat cgcatgagtt catggttcaa aaaattataa tcaaaataaa tattatgcgc
    19861 gacaaatatg gtgtctgcca aaagattgta aatcgtttgg gcgacatctt cgaaataagg
    19921 cgctttttgg acgcgttgat tcgtgatccc tgttaaatgc tggatttgtt ttgaaatctt
    19981 ccgccctgga tgaatatcga tcgaaaaacg tgaagtgatc cgacctgatt caatcatgac
    20041 gcagccaaat tggatgatcc gatcttcttt tggattcgtc ccagtcgttt cgatatcaac
    20101 gactgcataa gttcttggtt tcaaaatatc ttcctcttct cctgcctcta atgttctttt
    20161 gaaattatac tacaaagtgc agaaaaatac tctcatcgct ttttaaagaa aatagacaaa
    20221 aacgcccaga cacgcaaatg tctgggcgtt tattttttag tataggttaa ttaagcttta
    20281 gtaacgtttg ttgcttgagg tccgcgttgg ccttcttcaa catcgaaagt tactgcttgg
    20341 ccttcttcta aagttttgaa tccgtcgcct tggatagctg agaaatgaac gaatacatcg
    20401 tttccgtttt ctgcagtgat gaatccaaat cctttttctg agttaaacca ttttactgta
    20461 cctgtttcca taataaaaat cctccttgtg tatcaacaca tattttgcaa ttacttgaat
    20521 ggtgtatatc tggaagatgt ttatttgaaa caagctacca cagattaccg aacaaaaact
    20581 actcctatag tatatcatac cgtttacaaa aaagctagag tttttcgact ttttttatta
    20641 aaaaaagttt tctcagcgat tacataaagt gaaaaaccga acttttttta aaataaacga
    20701 catattgttc agttggtgtt tttttgctct gatgttcttc tttaaactaa aaaaattatt
    20761 ctttctgact acttatcggt tctgtttcaa cggtcgaggc tggggaagat tctttagtag
    20821 gtctgatgtt ctcatggtat ttctgatagc ctatgacagt taccagaaga acaaggcaaa
    20881 ccaatacgat caacgagatt agtttttctt ttttgctcat atctatacct cataaataac
    20941 aggatgatgt ccatcatatt tctgagtatg agtaaaagca aaagctgcag ctgtagaatg
    21001 taagaaatgc cagcaacttt cataatcaca ggtcggacag taccaaaagt aaccaaatct
    21061 tggctgcaaa gaccgttctt ctgtttcagg aatcgcagaa tcaagatctg ttgtctgtac
    21121 ataaacattt ttgtacagac gtgtgcctgt aataggagac cgctgtgttt cttcagcaaa
    21181 aactggctga cttgttaaac ttagggatag aaacagcaaa caccataaca gtcttttttt
    21241 cataaagacc tccttatcga taagtttttc ccaattaaaa tcatatctca agaacgcccg
    21301 ttattcacat tttgttcata aatgatgttt tttgtatcat aatcgtcaaa aagaggtcgt
    21361 gaccacgtca caacctcttt tttaaacggt gttccagaag tagcttcttc ggaaataagt
    21421 cgaaatttca caaaaatttg agaaataatt tttgtcaatc tcttcttatt tcttgaagct
    21481 gaccacttct gtcacaacct cttccttaaa cggtgttcaa aagcttgcat ctgcggtatt
    21541 agctcaaaaa acggaaaaat atgtgaaact atttttccgt ttgtttctct aatactcagg
    21601 tcaaaacact gatttcacaa cctcttttaa taacgaagaa tcgctgtcag actcttttca
    21661 tctggcgcat ctttttgatc caaaataaac actttcccgc cattaatgat cacatcatct
    21721 gctaatgcat tcaacacttg acgccgatca tattctgttt caggatcttc accgaaacca
    21781 tcagctaagt ttgacgtagc gataaataaa tgtgacactt ttccttcttt cgcagctgga
    21841 acgatatcaa ctaactgatc gatgaatttc ttatctaaga gtttattgta ttcttttgat
    21901 tcttgagctg cgagttcact tgacagcttc ttcgcccctt cttcgatatc tctaaaggat
    21961 agctgcgcag gagaagcaga aatcgcaacg tctgcacaat aatagggatt tttagcaatt
    22021 tttttgaaca tcgtctgatt ttctggtaat gcaaataaat aaatcggcca ttgttcagga
    22081 ttttcgaaag aatccttcaa gtacgtatca acagcttggt aatagttgac ccaatcgatc
    22141 tctacttctt catcttttgg atttacgcca tggaaagtta ctccatctcg ggctccgcct
    22201 cctccattag aagagaagtt caaaccgcca cctgtcagtt cagatcccaa tgcagtaacg
    22261 acatcaaccg gtgccccttc tggtagagat acttctttta cttgtttgtt gctgacctca
    22321 tacaatttca tggagtcgcg gttcaatccc aataaataat aagaatagtt gaactgccca
    22381 tttttgataa tagccagtaa ataaggaaga tcacttacat agtattgatc gtctaccggg
    22441 atactcaaac gatggatata ggtctggtca gcagttataa tgaccgctac actcgtagtt
    22501 gcactacgcc aaaacgaagg atctcctaac aaattttcaa ttttttcttg gaacggggac
    22561 cagtcatgtt cggaatattt tttctcaaaa cgtttctttg cttctttcgc aaaatttttg
    22621 aatttgatct gatctttttc cacatcttga tgggcgacat gcgtattcag catgatcgtg
    22681 acgaatggtc ctttagcgcc ttctgaagtc aattgtgtca ataattcttt atcctttttt
    22741 gccataaacg tacccctcct aaaaaaatca ttcctttgtt tctatctaaa gtctaccaca
    22801 acactatttt tccatacaat aaaacgtatc taaaaaaata aaacggtttc tacggtttta
    22861 aatttagcta tttttttaag ttatcttcat aatttcctca attatttagc cccagtcttc
    22921 aactattctt catgaaatgc tgataaacta agaaaaagaa taaaagaggt ggatcttatg
    22981 catttgatta tgattttcag tttattaagt attatcagtt tatttgcttt cagcgcttcc
    23041 cgcttattag atgcggaaac acagcctatt cctgtaaaag tacgtaaaaa cgaccgctaa
    23101 acttccataa cgcgcgttct tatcctaaag ttttttcaga aacattgaaa ctctctcccc
    23161 tctctgataa aattttaatt tgtagcgaaa caaattaatt acaaggagat cggatatgtc
    23221 cataaaaaaa acacgtctag tcctattatt ggctagtctc attctttgcc ttgcttcttt
    23281 tctaccagct gttgctgtgc aagctgaaga tacgttcaaa gtaaatgcaa aagcagcttt
    23341 tgctgtagat gcgcaaagcg gtaagattct ttatgatcaa gatggagaaa aaaccatggg
    23401 gatcgcttca atcacaaaaa tcatcggtct ttacatcgtc cttgatcaag taaaagaagg
    23461 aaaactgtca tgggatgata aagtttcgat ttcagattat gcagaaaatt tgagtatcac
    23521 cccagattta tcgaatgttc ctttgcataa agaaaacacc tacactgtca aagaattatt
    23581 cgactctgca atcatccagt cagccaatgc ttcaatggtg gccttggcag aaaaaatctc
    23641 tggcagcgaa gctaaattca ctgaacgaat gaaagaacaa ttgaaagatt ggggaatcaa
    23701 agatgcaaca atcgttaatg catcaggatt gaacaactct tatcttggcg aaaaccgacc
    23761 agaaggcact ggagaaaacg acgaaaatca aatgtctgct caagatgtgg ccatcgttgc
    23821 ccgccatttg atcttggatt tcccagaaat actggatgta tcaagtacta cgacccaaat
    23881 gtttggcgaa aatacgcaat caccagtgga aatggtcaac tggaactgga tgctcccagg
    23941 attcgttaat tacaaggaag gcgtggacgg attaaaaact ggtacgactg agcttgcagg
    24001 tgcttgtttc gttggtacga tcactaaaaa cgatcaacgg atcatcactg tcgtattgaa
    24061 tgcggacggc catgcggaaa acccgagtgc ccgcttcaat gaaacagacc gtttgatgga
    24121 ctactgctat gacaattggt cagaaaaaga gctaggaaaa gctaacgcaa gtattcctag
    24181 tctaaaaacc attgatgtaa aagacggaaa agaatcaaca gttcctgttg tgcttaaaag
    24241 ccctgtaaaa gtatgggtac gaaacgatat ggatactggc aaattaacga tcacgccaac
    24301 acttgacaag aagcaagtca ctgacaatga gatccaagca cctatggata aaggtacaaa
    24361 aatcggtacc gctacgatca ccttggcaga cgataaatta ggctatttag aagacaacaa
    24421 tgcaccttca acagagatca ttacggataa aacagtggaa aaagcgaata tattcgtgat
    24481 tggctggcga cgcgtcgcag aatttttcgg caacttgttt taattgaaca ataagaaaaa
    24541 catctttcca tcggaaagat gtttttttgc tttttctagt ttaaataaca gcttttttca
    24601 tctattttcg ttatactgta taagaaaaag gagttgaatt tatgaatcta accagagatc
    24661 tgatcttttt agttctctat cttattgggg tattctctat cgtttattgg cgaaaacaga
    24721 agaaggatcc ttccttgttc ttttacggca gtacgtggac atttacggta atcttaactt
    24781 attttgtcgt gttggaaatt ttttattcga ccttttattt tttgattcca ttgttgttct
    24841 ttagtatctt tgctttttct tatttcacag aaaaacgaag gctgctgaat ggtcttttat
    24901 tcaatgtttt cctcatcagt ttcggcatct acttgtttgt cttgctgtat gaaacgcaaa
    24961 atattttcct tggcggactg atcgccttga tcacgattcc attgctactt gtccttttat
    25021 ttggtattta tggattgatt gtttttcttt tttggaacgg ggtcacagtt ttacgtagag
    25081 aatcgcattc attagctaat cttttaacgt tgatactagc catttttctt acactgtttc
    25141 ttgtttttga ctttttcttg ttaaaatatt tgccgcaatg gataaatgct ttgtttttct
    25201 gcgttcccct gatcttgatc tatctattta tcgtattcta caactttttg actgtctcat
    25261 tcttgtatca gttcaaccgg cctcgataca atcaagactt catcgttgtg ttaggcgctg
    25321 ggttgatcaa tggagaaaca gtttctcctc ttctagccaa gcgaatcaat aaggcaattg
    25381 ctttttatcg ggctcaaagc cgtgctacat taaatccgcc catcttattg atgtctggcg
    25441 ggcaaggagc agacgaaaaa gtaccagagg ccattgcgat gaaacaatat gccatggaac
    25501 aaggaatccc tgaaagagac attcttgtcg aaaccaattc gacaaccact ttggagaaca
    25561 tgctctattc aaaggaaatc atggatcagc aaatgaaagg gccataccgc gcgatcttta
    25621 gttccaacaa ttaccacatt tttagagccg gtctttatgc gtggcaagcc aaattgaaag
    25681 caaatggtat tggtgcaaaa acagctttct actacttgcc taatgcgttt ttaagagaat
    25741 acatcgccat cttgatgatg aacaaaaaaa gacatatgat catttgcgga ttgcttttcg
    25801 caggctccgc attcttatcg ctgattactt tacttttcta aaaaaacaag gtgtgacaaa
    25861 acatatgtca caccttgttt ttttctttca ttctcttaac tattggcaaa aagagtatcc
    25921 cttttttgac cgctacttgc tgagcagaat aaataccgct gttgcctgaa cacataaagc
    25981 tgatcaagca gatcataaag aagtatactg ccgcttgtga accgaacaat tcgatcccca
    26041 tcacaaagca ggcaatcggt gtatttgtcg caccagaaaa tactccgata aagcccaatc
    26101 ctgcaagaaa aggaacagaa acatggagca aaggcgctag tgctgcacca agcgttgcac
    26161 caatttcaaa caatggtgtc acttctcccc cttgatatcc cgcgcctaaa gacaaaactg
    26221 tgaagaataa cttcccaata aaatcataga catgtgcctg tccattaaat gattcttgaa
    26281 gtaacggaag actcaatccg agataacgtt gtgtttgcaa aatcaatgct gctgccacaa
    26341 ctacagctcc acctatcgta tttcgccaaa caggatgagg aatccattgt gcatatcgcg
    26401 cttttaaaaa gacgatcgaa cggctgaata cccaaccaat caaaccaaaa gcaatcccag
    26461 caacaaaaac tttgaagaac agcaacatac tccattcagg aatcacaccc atttggtaat
    26521 gtagatgaga aacaccatag ctttctgtca tgaaatttgc aaacagtgca gcaaagaagc
    26581 tagggaagat cgcttctgtt cggactttcc cgattgccaa cacttctaat ccaaataatg
    26641 ttccagctaa aggtgtgcca aatacagaac taaaacccgc actgattcca ctgataatca
    26701 agatttcccg ttctgattta tccaatcgga acaaacgtgc tgtattatct gctaacgctc
    26761 cccccatttg gactgctgtt ccttcacggc caacagatcc cccaaataaa tgggtagtta
    26821 tggttccaaa cagtgtaagt ggaataagtc gcaacgggat tttttcttct ccaccatttc
    26881 cttgatcgat cactaagttg tttccacgac tagaaagacc gccgtgatac gcatagagat
    26941 aagcaaaaag cgcaccacta actggtagta gataaaggag ccaaggatgt gatagtcgga
    27001 tatctgtcac aactgtcagg ctatttaaga aaaaagcaga caagctcccc atgaatatac
    27061 cgagaatcgc tgtgatcacc agccacttca gcatgtacac taaaatgcgt actggactcg
    27121 gtaattgttt ttccataaaa aattcctcct caataaaaaa acgcctacta aagaaagggg
    27181 ctgtccccag actttagtag gcgtcattag ctatctttcg ttagcattta aaggcgaaca
    27241 ccatcaccta ttcacttcca ttaaaagata gcatattttt ctgtaaaaaa caataaaatt
    27301 atcgttcttt ctttacacgt tcagcaaatt catagaaagg agccaatttc gttaactgtt
    27361 gattacattg gtcactatca tgacaaatat aattcccctt tttcgtatac gtgccatcag
    27421 aacctgcttt cgttgtagaa agaaaaagcg aaacattcga tgttttctga cagattgcac
    27481 agattccctt atttatcgtt ggtgataaag taccactaat cccttttaat ttttcatctt
    27541 cgtaatagat catgaatttt ttctgcgtac ctggatcatt ccagccgatg tacgtatgtt
    27601 cttgcaagtc gatttcattc caagctggtt gtttgagttt tttcactttt cggaataatt
    27661 tctcgatttg tggtttgcta ggctgcataa acggctgaac gatttgcttc agctcctcca
    27721 atgctcgttc tgcttgcgct tttgtcagtt ttacgtctaa aactgtcgtt aaaaatgtat
    27781 cgatttctgg cagttcgtgt tctaatatgt ctcgaatctt ttcagttgct agtgcctgaa
    27841 ctgtttgtac cgtttttgga tcattgacag atgagtacgc atttaataac tgttctactt
    27901 ctctttttat aaaaaaatat tggtatggtt ttattgtttg ttccatgatt ttaaagtcct
    27961 ttcttctaac caaagtgcat actttgtaga aaaaagtgat cttctgcaaa gtatgtgtta
    28021 cctgaagatc ccggactctt ttaaataaga tacgttttgc atcttatcat cacccccttt
    28081 aatgcttatt cttctatgta catgattcat tcgataaata aaagtaaacc gtatcttgaa
    28141 cagatagtca actgcaaaca gaaaaaagga ctgtgacaaa agtcgtgtca aagtcccttg
    28201 tttcgaatga atggtgttca aaagcttgca tctgcggtaa taagccgaaa cttcacaaaa
    28261 atttgagaag taattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
    28321 aacctcaata tggtctatta ttggttttac ctgttaaatc gaggattctt tcccaaagta
    28381 acgttgccat ctcttacgca tacttgcaga catgggctga gccgcttctt tgactgcgca
    28441 gtatttgtcc acgaatgtca caatatatcc ttctttatac ttaggcggtg cgatcgttgc
    28501 accccacatg tgcttcaaaa tgatatctcg ctcaagatcc gacaaatcgg tgattttttc
    28561 tgcattcttt acagcaattc ttggatggat atacgcatgt gtcccctcgt caaatttcgt
    28621 cgtacgccaa tcatagtaga acagatcatg caaaagtcca gcacgcgctg ttgctttagc
    28681 attgccaccc catttttttg cgagcagata actatagtaa gaaacactga tggaatgctc
    28741 caaacgagtg gaatgaacat gctgtgtgta attcgctaat tttttcactg cttctgtttc
    28801 taaaagatct tgtacataag acatatattc ttcgtcttca cgccattttt ctgtcataat
    28861 tatcgatctg ccaccttttt gaatttcatt ttcttctgaa attgatctaa gtataccacc
    28921 agtgttcgga ttattctagt cgctctagat aaaatagcta aagcttaata gttccttaat
    28981 cataaagaaa aatgataaag aacgatccct gctgcgactc cgacattcaa tgattccgca
    29041 tttcctttca ttgggatata tacgttctga tcagtcaaag acaagatttc ctgacttact
    29101 ccctgacctt cgtttcccat gattatagcg aaatcctctg ttttttctac tttgtctaat
    29161 gcatgtgctt cttcatttaa ttctgtaccg tacaccgtaa accctttgcc tttcattaca
    29221 gggatcactt ctgccaaatc tttttgtaac acaggcaaat gataattgct tccctgcatg
    29281 cttcgcagca cctttgtact ataaatatct gccgttcctt tacctaataa aacaccagcc
    29341 ataccaaacg catctgccgt acggatcatc gttcccacat ttccaggatc ctgtacatta
    29401 tctagtaaaa gccagcctcc tgaaaaatct ggcgtatctt cagatgttaa cgtcatcacc
    29461 gctaagatcc cttgcggtgt aggtagatct gacatagcgg tcatgacctc atctgaaact
    29521 agaaaagtag aaatctcttt gtttacagcc agccagtctc cccattcttt catacctctc
    29581 tgtgtcacaa aaagttcttt tatttctgca tcagacgacg cagcttcttc gatcagatgg
    29641 aacccttcca aaagatattc tgccttttct tctctgtatt tctttttatg tagctttttt
    29701 aattctttga tttttggatt ttttgctgat tggatctctt tcacattcct tcacctcacc
    29761 tgattatatc atggaatagg tgcttgagct tgtttcatcc taaaaaaatc ggtatcattg
    29821 atttataaaa caggaaagaa aggtgacgaa catgcgcaaa gttaaaatga atgtccaagg
    29881 acgtgtccag ggcgtgggat ttcgctatat gaccaagatg gttgctgatc agttaggcgt
    29941 gaccggaacg gtaaaaaacg aagacgacgg ctcagtcaca atcgaagcaa tcggaaatga
    30001 cgacatcatc caaaaattta tcgaagaagt caagaagtct ccgagcccaa gtggacgagt
    30061 gcagtatgtg gatgtccaag aagatccgat gattgaggaa cgaaagaaat ttgatgtggt
    30121 gggataaggt tgtgaaaagg tcgcttagtt ttgagtatta aggaaaattt tgtgaaaatg
    30181 gctttccaca tttttgcata aatttgactt aataccgaga aactgacttt tgaacaccgt
    30241 cgatcaggtt gtttttgagg cgctttgcct caagtattaa agataggtag taaaaaagct
    30301 gatctataac ttgcaataaa tttgcttaac ccatattttc cagacagaac aaaaaagttt
    30361 ttagtttttt atataagaaa gaaacacttg gaatcactct tttctttcca agtatttctt
    30421 tcttgatttt ttgtcttttt tcaaaatttt tcgctacgaa aaagaaagaa tatttatatg
    30481 aaatttctat gaagttgatt gaattccatg cgctttttta gtatagttaa gtttgtgtaa
    30541 aaattataga agaggaagta ttctatgaat aaatggaaaa actggttatt gggatccgga
    30601 cttgtcagta tcctgctctt tttatctggc tgtgtgcgga tggattcaaa cggaaaccct
    30661 gacacttcag gaatcgttta tcgagtactc gtccatccta tgggccaagc gattacatat
    30721 ctagttgaga atttcaattg gtcttacggc tgggcagtta ttgcaatgac tgtgatcgtt
    30781 cgtatcatca tcttgccact aggtatcagt caatcgaaaa aaacaatgat ccaatctgaa
    30841 aaaatgcaag cgttaaaacc acaagttgaa gcagcacaac aaaagctaaa agctgcaaca
    30901 acacgtgaag aacaaatggc agcacaagca gaaatgcagc aagtatatcg cgaaaacgga
    30961 ttgagtatga ccggcggaat cggctgtctg cctctattga tccaaatgcc gatcttctct
    31021 gccttgtatt ttacggctcg ctacacagaa ggtatccgtg aatcatcttt ttacggcatc
    31081 gatctaggct caccaagtat ggtgcttgtg gccattgcag gggtcgctta tctgcttcaa
    31141 ggatatattt caacaatcgg tattcctgaa gaacagaaaa agacgatgcg tacgatgctg
    31201 atcgtctctc ctgctatgat cgtcttcatg tcgatcagtg cgccagccgg cgttacactt
    31261 tactgggtag tcggtggtgt gttcagctgt ctgcaaacat tcatcacaaa cgttatgatg
    31321 aaaccacgct tgaaagcaca agtggcagaa gagttgaaac aaaacccgcc aaaacaagtc
    31381 gtcactccaa gaaaagacgt gacagaatca gcaaaaccag ctgacaacaa acaagcaaca
    31441 aaacaaatca aacaaccaaa aaacagtaca aataacaaag gccgcaacgc aggaaagcag
    31501 cagaagcgga agtagatagt aaataagagt gtaataaaaa ttcattaaca gttttagcaa
    31561 ttaaaaatat cgaccaagat ttttgaaaaa gaaatctagt cgatattttt atttaagtga
    31621 atgtataatt tgaatcatcg attgatcgct atctaattgc agcagcatct ttttatgaag
    31681 aaacaacttt ttcttatcta tcaacgtaga caggtgttgc tgtttctttt aatagtgtac
    31741 ttaattcctt aatattttgt tttccttctt ctaacatcca actactcgct ttttcttcgc
    31801 cttcggtaac aaaaggtgcc actcccttcg cccatgttgc tcgcccacat aagacaccat
    31861 tgaatgtcga acctgattgt tttgcaaaac gtaatgtatc ttgaaataac gtagcattga
    31921 ctcccgcact taaaaaaata aacggttgtt cagtcacttt cgattgttca tagaaatacg
    31981 cggatgcctc tgcttgacta taacaagtct cttgcccata tccttccaca aatctcatat
    32041 tcactggcac ttctactttc aatacatcca cttgataacg ttcttttgaa aattctcgca
    32101 tggcttcaat aactttatgg ggttttactt ttgcataggc aagagaggac ggatcgccaa
    32161 tagttgcatc ataagaaagg atctctaaaa agaaaggtat gccttctgca tcgcattctg
    32221 aaccaatccg ttctataact atttgttttt gcacattaat ctctgctggt tcatccacgt
    32281 catagtacaa taaaaattta caagcatccg caccagcttc ttttaacctt ttcaccgacc
    32341 aaatattcaa taaatcaggt aatcttcccg gcattttcgt atcatagccg gttttttcat
    32401 aagctagcag caagccacat tctttctctc taagcgctgc tgcaggcagg ccgcattctg
    32461 gatcaagtaa aatagctgag gcatagggag tcaggtattg agaaaccatc tgtttaaatt
    32521 ggatgatgtc ttctgatgta gcttctttat attggttgat cattttcttc aaagcaccac
    32581 gctggtcgat tgctaacgcg ctaataattc cagcttcatc cgtcaatcgc ttcaaacgtt
    32641 cctgcttttc tatactcatc ttcttcatag atcttcacct atttctttag ttaatattgt
    32701 ggctatttct gtttttgatt ttgcttttct caactgttct gtaacctctt ttcgcatcaa
    32761 tgatcgtgca acgagagaca ataatttcaa atgagtcgtt cctgcttctg aatctggaat
    32821 aaataacgca aaaataaaat caactgactg tctatctaaa ctgttccatt caattccttt
    32881 gttcagtgtg acgataatca tactaggttc gttgattgtt tgagacttcg catgagggat
    32941 agcaaaacct gacattattc ctgtagtacc ttctttttct ctttctagaa gttttcgata
    33001 tacttctttt tcgtcatgag cgattcctaa acagaatgtt tgctttgcta aaaacttaaa
    33061 tgcttcttct tgactatcta atggctgttg taaaaaaatg ttctcttcat gaatgattgt
    33121 catgataagc ctcctgctct tccttcttcg ataactgtac gatttgctgc tttattatcc
    33181 atcttcttgt ctttatcagt tactcttttc ttttggatca aacctagtat cactccacca
    33241 acgatcgaac caataagaat cgctgtaacc cattggattt ttcccgtcac tacaggtaac
    33301 actaaaaaac cgccatgtgg ggctggtact tggacaccaa acagataggt caagacagca
    33361 gcaattgatg agcctaacat catcgttggt aatactcgga tcgggtcttt cgcagcaaac
    33421 ggaatagctc cctccgtaat atgagttgat cctaaaataa aattaaccaa acctgcatta
    33481 cgatcattag tatcaaaata tttaccaaac aataaagttg caaaaccagt aatcaacggt
    33541 ggtgcaatgc aagctactga cacgccagcc ataaaagtcg tgtttccttg tgccaaaaga
    33601 gctgtacctg ttacatatgc cgccttatta atcggtcctc ccatatcaaa cgcacacatg
    33661 caaccaacaa tcacgccaag aacaagtgga ttcgaatgtt caaatccagc taaaaagctc
    33721 atcattcctt catttatagc cttcattgga cccgccagca aagacatcac gatgccgata
    33781 aacactacac caataactgg atataagaag attgccttca agccatctag ttgtttaggt
    33841 aaaattgtca gtagcttttg caaaaataag ataaaataac ctgctaagaa cccagctaaa
    33901 atacctacta aaaatccagt accgccactc atggccacga ttccaccaac aaatccaacg
    33961 actaaccctg gacgtttcgc gatcccttct gcaatataag ctgctaaaac tggaaccatc
    34021 atccccattg ctgcatcgcc aaccgttttt aacatcgccg ctgtagcatt gtattgtgca
    34081 tggtctggat caaaagaata gattccccaa acggcaaatg aaatagcaat caatactccg
    34141 ccagctacca cgaaaggcaa catatgagag acgccattca ttaaattttt atagatgctg
    34201 cgaccaaaat tagcaccact tatcgtttct gattctaaag tatcaacagc ttcattatct
    34261 gctaagatgg tcccttttcc tgccaatgct tcgtcaatca gccgttgtgc atccttgatt
    34321 cctgtactga cagaaacatc aatgactcgt tttcccgcaa atctttctga atggacatct
    34381 ttgtctgcgg caataatgac agcatctgcc tgtttgattt cttcagccgt taattcattc
    34441 tcgatcccaa tttgtccatg ggtctccact ttgatcgtga tgcctttttt tctagctgct
    34501 tgctctaatg cctcttgcgc catgtaggtg tgtgcaattc ctgtcggaca accggttgct
    34561 gcaattagtt gatattttcc catctttcga acctctcttc cttgattttg atttgtttac
    34621 ttaattcctc cacatccaaa aaatcagtca gtcctttacg aaaagcagta gagctgcctg
    34681 ctgctatgct tttacgtaac gtttcatcaa gcggtcgtcg attcatgtat cctactaaaa
    34741 aagtgcctaa catggcatca cctgcacatg ctgtattgac aacttttcct tttggcgagt
    34801 ttcctcgaat caccctgtct ttcgtaaaaa gaatcgctcc tttctcacca agggataaca
    34861 aaacattttc tgcacctgac tctaccagtc gttgtccata aacaaagtaa tcttcatctg
    34921 taaccatttt tcgtccgaac caagaagaaa gttcttcctc attcggtttc agcaaaaatg
    34981 gtcgataggg caaacaatcc atgacctctt gatcactgct atcaataatc aaatgaatct
    35041 ccttttcctg acaaatccga ctgatttcca ctaaaacctt cggagacatt cctcttggta
    35101 aactcccaga aacacaaaga taatcctctg cttgtaatgc tcttatttga tttaaaaaat
    35161 tatggacagc tgcctgcggt atttcaggtc cttgattcac taatttgtat tcttctctcg
    35221 tttgattgac ttgtgtaaat acattgatac gcgtcatacc aggcacttca ataaactctg
    35281 tttggatttg tttcttctgt aaatagtctt ctatatattt tcctgtgaaa ccagcagaaa
    35341 aacctaatgc cgtacttggg aaatctagca tctttaatac tagtgacaca tttactcctt
    35401 ttccgtttgc ctgaatgtca tcctcctttg tacggtttac cataaaagga tgcatctcct
    35461 ctgtttcaat aaatagatca attgccaaat tcatcgtgca tgtgtagata gacataacca
    35521 atgacctcct tgaatctatt atgaaatacg cgaaagagaa aatgttttct taatcgttcc
    35581 aacacaaaga aaacctttac atttataaac acatcgtgct attttttaac taacttttag
    35641 tgaaagaagg aaagaatatg gatcacaaat taagtgaaac agaacagtac ctctggaact
    35701 ttatcgaaca ccacatccta gagatcccta actactccat cgtgaaacta agtgaacaag
    35761 caaatgtctc aacagccacc attgtgcgga caatgaagaa aaaaggctat gaaggattta
    35821 cttcgtttaa gcaccatcta aaagaaggag aaaacaagaa catcaatttt tctttttaga
    35881 taaagtagac aaaggtatcc gtcgagccat tctaaaaaat gaacaagaag tggtacgtac
    35941 gatcaatatg ttggaaatcg ggaacattga agatgcaata caaagaatca aatttactga
    36001 ccgggttttt gtctttgccc gaggattttc agaaatgatt ggtcaagaga tgctcgtaaa
    36061 acttcaatta acaggaaaaa attgtcaaat gcatactgat ccagagatta tcaaaagcat
    36121 cagtcggaaa ctaactaaga aagacagcgt tatttttgtt tctttgaacg gcgagacaaa
    36181 agaactcgtc accgccgcaa agaactgcta tgatgcagag attggttcga ttctagttac
    36241 agccaatcat tcctctacac tcaaagatta ttgcgagata aatctcgtag gtttcaaatc
    36301 agaaggttct tatttccctg attatgaagt acgttcacgc ttgcctctcc agatcatcgc
    36361 gagaatcctg cttgacgcct atgtattgcg tatgagggag aattgagaag cagtttatta
    36421 tggttggtta cagtttaaaa catcacattt tttattcttt atattgtatg gactcacgta
    36481 aactttttat gtataaagtt attcacaatc tataaagtta ttttaagtaa caaaaaataa
    36541 ttgaaaatac ttaaaatcaa aaaatatctt tttaatcaat aaaaaaattg acagttttat
    36601 taataataag ctattctgtt atagaagaaa aaattgtttg ttattattag caacaaaaaa
    36661 aggagtgcat catgaaaaag ttatctcaaa aacgtttagc tcaaatcatt attgattcta
    36721 gaaaatctct gaaaatgaca caacaacaac ttgcagtagc tactggtatc aaccgtgcct
    36781 taattagtcg agtagaatct ctcgatttta tcccgtcaat acaacaatta gaatcattag
    36841 cagaagttct aaattttgaa ccaacagatc tatttataaa tgaatcaaat aaatcaaaca
    36901 gaacaaaaca tcagtcgaag aatattgctg tagccggtac tggttacgta ggtctttcca
    36961 ttgctactct tcttgcccag cacaatcagg taacagcagt agacattctt cctgaaaaag
    37021 taaaacaaat taacaataag aaatctccaa tacaagacga ttacattgaa aaatatctag
    37081 ctgaaaaaaa tcttaattta attgcaacga ctgatggtgc agccgcctat agagaagccg
    37141 attttgttgt cattgctgct ccaacaaatt atgatagtaa acaaaattat tttgatactt
    37201 ctgctgtaga gtcagtaatc aaattggtgt tagaagtcaa tccaaaggca ataatggtca
    37261 tcaagtcgac ggtacccgta ggctataccg aaagtattcg taaaaaatac aatactaaaa
    37321 atattctatt cagcccagaa tttctcagag aatctaaggc attatatgac aatctatatc
    37381 cttcgcgaat tattgtcggc acggaccaaa ctgacaaaac tttacgagaa gcctctcaca
    37441 cttttgcaga tttactccaa gaaggagcaa tcaaaaaaga tatcgaaact cttttcatgg
    37501 gattcacaga agctgaagca gttaaattat tcgctaatac ttaccttgca cttcgagtat
    37561 cttacttcaa tgagctggat acttatgcag aaatgaaagg actaaataca caagaaatca
    37621 ttcacggggt ttgcttagat ccaagaattg gcacacatta taataatcca tcttttggat
    37681 atggaggcta ttgtttaccg aaagatacaa aacaacttct cactaactat aacgatgttc
    37741 ctcagaatat gataagtgca attgtcgata gtaatcgtac aagaaaagac tttatagcag
    37801 atcgagtgtt agaacttgct ggcacgtatg aggctaacag ttcgtggaat gcagaaaaag
    37861 aaaaagaggt agtcataggt gtataccgtc ttacaatgaa gagtaattca gacaacttcc
    37921 gtcagtcatc tattcaagga gttatgaagc gaattaaagc aaagggggca actattgtaa
    37981 tttatgaacc aacacttgaa aataacacta cattttttgg ttcaaaagta ataaataatt
    38041 tagatgattt taaaaatatc agtcacagta ttattgctaa tcgttacgat agtaatcttg
    38101 atgatgtaaa agaaaaagta tataccagag atttatttca acgagattaa aaaataacac
    38161 ctcagatttg tagtgtctga ggtgtttcta taaactttta taagtaaaaa aatacaatta
    38221 tacttcataa aaagacttat accactgcgc aaatttacga agcccgtcac gcaaacttgt
    38281 acttggttta aattcaaaat ctcgttctaa cgcactagta tctgcataag taactggcac
    38341 atctcctggt tgcattggaa ctagttcggt atgagattca aagtcgtaat cttctggtag
    38401 cacccctgct gcaattagct cttgttgtag aatatctaca aaatctagta ggttttccgg
    38461 cgtattgtta ccaatattat aaatatgata tggtgggatt ggtagcccat cttccccatc
    38521 aattttctct ggagcgcact gcataaccct ctttactcct tcaacaatat catctatata
    38581 ggtaaagtca cgcttgcaat tcccataatt gaatatttgg atattatcac cagcacgaag
    38641 tttatcagta aaaccaaaat aagccatatc tggacgacca gcaggaccat atactgtaaa
    38701 gaaacgtagc cctgtagacg gaatattgta aagtttagaa tatgcatgtg ccattgattc
    38761 attacttttc ttagttgctg catacaacga tacttgattg tctactttat cctctgtaga
    38821 ataaggaatt ttcttattag taccataaac ggaagacgat gaggcataca ctaaatgttc
    38881 cacaccagat tctccattat catatgaatg acgacaagct tcaagaatat tatagaatcc
    38941 tattatgtta gattcgatat acatatctgg attagtaatt gaataacgga ctcctgcctg
    39001 agctgctaaa ttcaccacga tacttggttt ataattatca aagaggaaat caattgttgc
    39061 tttatcagca atagaacctt tgataaaatt ccaagtagat cctggatggt tctttgcctc
    39121 cgtttctatc tctgataatc tatagttctt aatagagaca tcatagtaat cattcatatt
    39181 gtcaatgcca atgattttta ttgatgactg agtacgtaat aactcaagaa ccaaatttga
    39241 tccaataaaa ccggctgcgc cagttattaa tattgtttta ttctgtaaag atgtattttt
    39301 tttcagcatc tagttatact cccttatttt tatttcatca tatgaattaa ttaaatgtct
    39361 tcataatttg caacaagtta tgttcaaagg catcataact atatagttct gctctctttt
    39421 ttgctgcttt tgcatactta ttcctcaaat tatcgttttc aatcaattct tctatagcat
    39481 tcgctaaatt tttatcacat tcttcatatt ggggcgaata atttctactg tctgtcattg
    39541 gacatactaa tatgcctgat tcagcatatt ctatattttc acaaatcgtg tctagcgctc
    39601 tattcgataa tatttctctt ggcccagatt tgcaatctgc acataagaca gcagtttctg
    39661 tagacattgc ttcaactaat gcattaggaa aaccttcagt tatagatggc atgacatata
    39721 aatctgcacg tgacatgtat ttataaggat ttgattggcc acctagcaaa aatattacgt
    39781 tttgtagttg atatgctttt attaaatttt ctaataattc ttttt
//
LOCUS       DACDIH010000021        35652 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDIH010000021 DACDIH010000000
VERSION     DACDIH010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.021"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            337..2502
                     /gene="acm"
                     /locus_tag="IV281_001133"
     CDS             337..2502
                     /gene="acm"
                     /locus_tag="IV281_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP8780160.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2774..3259)
                     /locus_tag="IV281_001134"
     CDS             complement(2774..3259)
                     /locus_tag="IV281_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP8780161.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3739..4380
                     /locus_tag="IV281_001135"
     CDS             3739..4380
                     /locus_tag="IV281_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8780162.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4399..5034
                     /locus_tag="IV281_001136"
     CDS             4399..5034
                     /locus_tag="IV281_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8780163.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            5052..5867
                     /locus_tag="IV281_001137"
     CDS             5052..5867
                     /locus_tag="IV281_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8780164.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6186..7622
                     /locus_tag="IV281_001138"
     CDS             6186..7622
                     /locus_tag="IV281_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8780165.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7811..10258
                     /locus_tag="IV281_001139"
     CDS             7811..10258
                     /locus_tag="IV281_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP8780166.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10488..11555
                     /gene="rlmN"
                     /locus_tag="IV281_001140"
     CDS             10488..11555
                     /gene="rlmN"
                     /locus_tag="IV281_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP8780167.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11748..12851)
                     /locus_tag="IV281_001141"
     CDS             complement(11748..12851)
                     /locus_tag="IV281_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8780168.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13404..13952
                     /gene="aac(6')"
                     /locus_tag="IV281_001142"
                     /pseudo
     CDS             13404..13952
                     /gene="aac(6')"
                     /locus_tag="IV281_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
     gene            14498..16159
                     /locus_tag="IV281_001143"
     CDS             14498..16159
                     /locus_tag="IV281_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8780169.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(16229..16576)
                     /locus_tag="IV281_001144"
     CDS             complement(16229..16576)
                     /locus_tag="IV281_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP8780170.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            16660..17601
                     /locus_tag="IV281_001145"
     CDS             16660..17601
                     /locus_tag="IV281_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8780171.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            17602..18645
                     /locus_tag="IV281_001146"
     CDS             17602..18645
                     /locus_tag="IV281_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8780172.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            18651..19721
                     /locus_tag="IV281_001147"
     CDS             18651..19721
                     /locus_tag="IV281_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8780173.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            19711..20664
                     /locus_tag="IV281_001148"
     CDS             19711..20664
                     /locus_tag="IV281_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8780174.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            20676..21686
                     /locus_tag="IV281_001149"
     CDS             20676..21686
                     /locus_tag="IV281_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8780175.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(21971..22792)
                     /locus_tag="IV281_001150"
     CDS             complement(21971..22792)
                     /locus_tag="IV281_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8780176.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(22889..23167)
                     /locus_tag="IV281_001151"
     CDS             complement(22889..23167)
                     /locus_tag="IV281_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780177.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            23435..25444
                     /gene="metG"
                     /locus_tag="IV281_001152"
     CDS             23435..25444
                     /gene="metG"
                     /locus_tag="IV281_001152"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP8780178.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(25923..26510)
                     /locus_tag="IV281_001153"
     CDS             complement(25923..26510)
                     /locus_tag="IV281_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780179.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            26685..28007
                     /locus_tag="IV281_001154"
     CDS             26685..28007
                     /locus_tag="IV281_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8780180.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            28162..28938
                     /locus_tag="IV281_001155"
     CDS             28162..28938
                     /locus_tag="IV281_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8780181.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            28931..29500
                     /gene="rnmV"
                     /locus_tag="IV281_001156"
     CDS             28931..29500
                     /gene="rnmV"
                     /locus_tag="IV281_001156"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP8780182.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            29493..30377
                     /gene="rsmA"
                     /locus_tag="IV281_001157"
     CDS             29493..30377
                     /gene="rsmA"
                     /locus_tag="IV281_001157"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP8780183.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            30828..31457
                     /locus_tag="IV281_001158"
     CDS             30828..31457
                     /locus_tag="IV281_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780184.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(31581..32690)
                     /gene="ugpC"
                     /locus_tag="IV281_001159"
     CDS             complement(31581..32690)
                     /gene="ugpC"
                     /locus_tag="IV281_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP8780185.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            32899..33321
                     /gene="mgsA"
                     /locus_tag="IV281_001160"
     CDS             32899..33321
                     /gene="mgsA"
                     /locus_tag="IV281_001160"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP8780186.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            33644..34180
                     /locus_tag="IV281_001161"
     CDS             33644..34180
                     /locus_tag="IV281_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8780187.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(34271..35566)
                     /locus_tag="IV281_001162"
     CDS             complement(34271..35566)
                     /locus_tag="IV281_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8780188.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT        11834 a         6187 c         7358 g        10273 t
ORIGIN      
        1 tagtataaac taataatgtt aattaaaagt gtattttatg aaactatcgt tatttattaa
       61 tgttgcattt ttgtaagtta aaaattggaa atatagattg aaaaaatgaa agcaataagt
      121 aaaataaaat gaatatatgc gaaaaaataa tttacattag gaaaaacctt gtaaaaacag
      181 ttttttggca tatttatttt ttataaatat ttgtaaaata actatcaatt ttttagttaa
      241 aagattggaa atgagtgggg gaggaattga agaaaggatt tttgagagat gatatagtag
      301 gttttatttt tagttaaaat aggggagata gaaaaaatga aaaagtgtag aaatattatt
      361 ttatcaatgt tatttattat tactaatata acatcactga ttcctgttca tgtctatgcg
      421 gatgcaggca gagatatcag cagtaatgtc acttcgttga ccgtggaccc aacgaatatt
      481 acagatggag gaaacataaa agtaaaattc tcgtttgacg aaaaaaaaca aaatatccaa
      541 ccaggtgatt atctttggat caattggcca agtgaaggaa atattcgcgg tgaaggattc
      601 caaaaagaaa tcccattaat gattgaaaat aaaaatgttg gaactttaac ggtcagaaaa
      661 gattctgcgc aggttgtgtt taatgaaaat attaaaaacc tagactcagt agaaggttgg
      721 gggcaatttg agatacaggc cagaaacgta accgataccg gtgaagaaaa tacgggacca
      781 ttcaccgtga cgagcggtga taaaacagct actgtaaatg tgacaaagcc agcttctggt
      841 tcttcttcta gcgtttttta ttataaaaca ggagacatgc tacctgaaga tactaagcac
      901 attcgatggt ttttgaatat taacaataac ggcacttatg ttgaacaacc agttaaaatc
      961 agtgatgaaa tccaaagtgg acaaagatta gatccaagta cgtttgaaat taatcaaata
     1021 cacttaggtg aacagaaggt ttatagagga gaagagggaa tccaacaatt tttacaagat
     1081 ttccccagcg ctacatttaa tttttcagta actgataatt acattgaaat aactatacca
     1141 aaaaactttg tcaatcttag gaaaataatg gtgagttata agacgatcat agaaaaccca
     1201 gaacaaataa attttgagaa tcattcggaa gcttggttta aagaatttaa taaacctgct
     1261 gttgatggag aaagtttcaa tcatacagtt aaaaatattt cagcttctgg tggagtcaac
     1321 ggtacagtta gaggcgaatt gaagattttc aaatatatca atgacacaga gattggtatc
     1381 ccaaatgtga ctttcgaatt aagacgagca gatgaacagc ctattcaagg acagtctagt
     1441 atcctgttga cttcgaatga acagggagaa gctagtatca aaggcctgca agtaggagac
     1501 tatgttgtaa aagaaaaaga agcacccaat tggattgatt ttgatccact aagttcaaat
     1561 gaactcaaat tctccataaa cgaaaatgat acagagggag tcagtttacc tatttataac
     1621 aagaaaaagg ttacgaacat cacagcaact aaaatttgga acggtggaac cactccaaga
     1681 ccgagtattt attttaaatt attcagagca agtacaaaca attgggaacc agttcctgat
     1741 gcagaaacaa aacgattaga caatggaata acttcagtga cttgggaaga tatccaacag
     1801 tatgatgata gtggaaacga atatacgttt aaagtgcaag aagtagatca aaacggaaat
     1861 gattatgtcc cttcaggtta tcaaaaaatt gaaaacgggc tagtcgttac aaatgagaat
     1921 aaagaagtca tttcagtctc aggacaaaaa acatgggaag acaatgagaa ccaagacggc
     1981 aaacgaccga cgacgatcac agtaaatcta ttagccgacg gtcaaccgat ccaacacaaa
     2041 gaagtaagtg aaaaagatga ttggcgctat cgtttcacaa atctgccaaa atacagagac
     2101 gggcaagaaa tcatctatac tgtaacggaa gacaacgtgc ccgaatacag tacgacgatt
     2161 gacgggtata acatcaaaaa cagttataca ccggaaaaaa caaatatatc tataccaggt
     2221 agccatataa caccgtggaa aaattttcca gagaagacag agagagagaa aattagaaat
     2281 ctattctctc aaaaattgca aatatttgct catacaggag aatccagcga acaaagaagc
     2341 aattatatta gtaagagagc tgtacctaaa cagataacaa ataaatcaaa aagcattctt
     2401 cctaaaacaa gtagtaaaaa atctagtttt gctgtaatca ttggtttatt gatagtaact
     2461 atctgtgctg ggatattctt acaaaaatat aaaaaagttt agttagaata taatattttt
     2521 tgttaaatat gactgaaatc aaaagaataa aattccgatt tataatcgaa aaaagaagca
     2581 tttttatata tgcttctttt ttcgtagaat caataaatat ggatttggct aactatggaa
     2641 gaatactcgt attagttttc agcgttcata ctagttgtta cattgtttga ttggccaaaa
     2701 gaatttctga tatattttgt gctaaataaa gaaaaagacg cttgaattgg gaaataagaa
     2761 aactactcta tcattacaaa ttttcataaa ctggcagatg ttctaagtaa acaagtatct
     2821 tttcaaagtc ggaaaaatcc ataatcaacg tagtggtatt gatatttgga tgaaagccta
     2881 cggtatcttg acgatcaata gcagcatcga tcacgacttg gatttttttc tctttgtcgt
     2941 gcatcaatgc taatggagtc actgtacctg ctggaacgcc taggaggtca ggaagctgtt
     3001 cttcagaaac aaaagataag cgcttttctt ttaattgttc agctagttgt tttaaatcgg
     3061 cgtgcttttc atcaggcaag atgacaagat agatttgttt tccttttctt gatttcaata
     3121 atagattttt tacttgcgga ccaggtaggg aaaaagggac atttttgact gacgtaatcg
     3181 caggatgatc gactttttga aaagagatac ccagtttttt tagtagttca taagcttctt
     3241 gttcggttgc acgactcatc gcgcatcacc ctcctttttt tattagtgta tcataaaaaa
     3301 gagttgacaa atgaaaaata atcatgtaaa ttacacgtaa agttaaaaca aatgacatcg
     3361 aaaagaagag tagccgttag gtgtttttag agagtctctg gttggtggaa agaggcagca
     3421 gttaaccgtg aaacacatct tggagtcgat tttctgaagc gtatagtagg aaaatccggg
     3481 gagcccgtta cagctgcggt cgttgctaga tgaccagata aggctggctt ttccgtgagg
     3541 agtcggtaaa aaaaggtgga accacgtgat taacgtcctt tgatcttcgg atcaaaggac
     3601 tttttgtttt catctgaacg gcaacgactg tgtgaggttt tttctgcaag gaaaaaataa
     3661 acagaggtgg aaccacgtat aatacgtcct cttggcattt gccaagagga tttttttatt
     3721 ttaaaggagg aaaagaagat ggagtatttg attgaagtta tgccgcaatt attagtagga
     3781 gcactcacaa cattgaaatt atttttattt accttgattg gctcattacc attagggatt
     3841 gttttagcat taggattgtc tttgcatttt aagccgctac gctacttgct tcaaacatat
     3901 gtatgggtta tgcggggaac accgctgttg ttgcaactga tttttatttt ttatgggttg
     3961 ccgacgattg ggatcgtttt tgatcgtttc gaagcagcac tcattgcctt tatcttaaac
     4021 tatgcagctt attttgcaga gatctttcgt ggagggatac aatcgattcc tcaaggtcaa
     4081 tatgaagcag cgcaagtttt gcaattaagt ccctttcaaa cggtgacgaa aatcgtcatc
     4141 ccacaagtga caaagattgt tttaccttct gtagggaatg aagttatcaa cttggtcaaa
     4201 gattcttcct tgatctatat attaggttta ggtgacgtaa tgcgtgctgg gaaaatcgcg
     4261 atggaacgcg acgcgacttt actgcctttg ctaggcgtgg caatcatcta tcttacattg
     4321 actggtattc taacaatcgt aatgaaacaa gttgaaggaa aattaaatta ttaccgctaa
     4381 ctgataggag gaagaaatat gattgaattg aagaaagtga acaaagcatt tggtgaaaaa
     4441 aaaataatca aagatttgaa tctaatcatt ccagatggga aaacattagc tatcgtaggt
     4501 ccttccggtg gcggtaaaac cacgctttta cgtatcttgg ctggcttaga aagttctgac
     4561 agcggagaat tttatttaga cggtgtaccg ttcaacccat tcgagcttaa aatgaaagaa
     4621 caagttgtag gagtcgtatt ccaagacttc cagctatttc cacatctgaa tatttttgaa
     4681 aacattacct tagcaccaaa aatggtattg aaacagacaa aagaagaata caccaaaaaa
     4741 gcagaagaat tagcggcttc attagggatt agtgatttat tgaatatcta tccttatcag
     4801 ctttctggcg gacaaaaaca gcgggtagcg attgccagag cattggctat gaatccacgg
     4861 gtattggctt atgatgaacc aacaagtgct ttagatcctg aattacgcca gcaagtcgag
     4921 cgtttgatcc tatcattgaa agaagatggg gtaacccaaa ttattgtcac ccatgattta
     4981 gtatttgcag aaaatgtcgg tgatcaatta ctaaaagttt ccccaattca ttaagaaagg
     5041 agaagataga gatgaaaaaa accagttttc tattcgcttt gatcgcaggt cttcttcttt
     5101 ttgcaggctg ttcgaacaaa aaaacttcag cagataattg ggcgactatc aacgaagaga
     5161 aaaaaatcgt tataggttta gacgatacat ttgttcctat gggatttaga gataaagacg
     5221 ggaagctcac aggatttgat attgatttag ccaaagcagt ctttgaacaa tatgggatta
     5281 cggtagactt tcagcccatt gactggtcaa tgaaggaatt tgaactgaac aatggaacga
     5341 tcgatttgat atggaacggc tattccaaaa cgccggcaag agaaaagaaa gtgcaattta
     5401 caaaacctta tatggaaaac gatcaagtgc tgatcacacc taaagaatca ggaatcacaa
     5461 actttggaca aatgaataat aagagattag gtgcgcaaaa tgggtcatct ggttatgatg
     5521 tatttacgaa acaaccagaa gtattaaaag agatagtcaa aaaccaagat gctgttttgt
     5581 atgatagttt taatgaagca ttgatcgatc tgaaaagcag acgggtcgat ggtctgctga
     5641 tggataaagt atacgcagac tattatttga aacaaagaaa cgaactagaa gacttcaaca
     5701 taacaaaagg ttcttatgag agtgaggatt ttgcagttgg tgcacgcaag tcagatagta
     5761 cattagtgaa aaaaatcaat gaagccctag atcaactaga gaaaacaggt gaattccaaa
     5821 aaatatccga taaatggttt ggcgaagatg ttacgccgag aaattaacaa ataggggact
     5881 gtgacaaaag acgtgtcaca gtcccttttt ccgaataaac agtgttctcc tgccaactcc
     5941 ttgtgactcg gagctgaatg gcaggctcac aacctcttgg taaacggtgt tcaaaagcca
     6001 gtttttcggt attaagccaa atttatgcaa aaatgtagaa agccattttc acaaaatttt
     6061 tcttactact cgaaactaag cgactttttc acaacctcct ttacataatt ttattttgtt
     6121 gtggaagggg tttcgtgtta ttctaaaaga ggaacgattg aaggttatag gaaaggaaga
     6181 gatgaatgga acacaaagaa cttacctcat ttccaaaaga ttttctgtgg ggctctgctt
     6241 ctgcagcgta tcaagtagaa ggagcttggc aagaagatgg gaaaggagaa tcagtctggg
     6301 atcgatttgt acggatacct ggaaagacat ttaaaggaac gaacggtgat ttggcagtag
     6361 accactatca tcgttataaa gaagatattg ctttaatgaa agaacaaggt ctgaaagctt
     6421 accgcttctc tgtagcttgg acacggattt ttcctaatgg acgaggagaa gtcaatcaag
     6481 ctgggctcgc tttttatgag agattgatcg acgaattgat tgaaaacgat attgaaccta
     6541 tgttgacttt atatcattgg gatcttccac aagcattgca agatgaatat aatggttggg
     6601 aatcacgtca gatcattgaa gatttcactc attatgcaga aacattattt gaggcattcc
     6661 gcgggaaagt ccattactgg attagtttga atgaacaaaa tatcttcact tcattaggtt
     6721 atttgttagc agcacatccg ccaggtgtga cagacccgaa acgaatgtat gaagttaacc
     6781 atattgctaa tttagcaaat gcatctgtca tcaataaatt tcacgagtta gaaatgcctg
     6841 gaaaaatcgg accaagcttt gcgtatacgc caaattatcc aattgacagt aatcctgaaa
     6901 atgttctggc tgctgaaaat gcagaagacc tgatggccaa ttattggatg gatgtctata
     6961 tgtggggaaa atatccgatt gctgccatga ggttcttaga agaaaaagga tgggcaccaa
     7021 cgattgaagc tggcgatgca gaattgctag aatctgctaa accagatttt cttgggatca
     7081 attactatca aacggcgacg aatgcattca atccattaaa cggagttggt gctggaaaaa
     7141 tgaatacgac aggaaaaaaa ggttcatcag aagaaactgg agttcctgga atgtataaaa
     7201 aagttgagaa tccatttgtc gaacgaacaa attgggactg ggagattgac ccagaaggat
     7261 taaggatcgg actgcgtcgt atcacaagcc gttaccgcat gccggtactg attacggaaa
     7321 atggcttagg agagtatgac aagttgacag aagataaaca aatccatgac gactatcgaa
     7381 tcaattactt acaaagccac gtcaaagcca tcaaagaagc aatttcggat ggagcagaag
     7441 tcctaggcta ttgcacctgg tcttatacag atttgcttag ctggctgaac ggttatcaaa
     7501 agcgatatgg atttgtatat gttgatcaag acgaaacaca agaaggatct ttggaacgat
     7561 ataagaaaga cagttattac tggtaccaaa aactgattga agaaaatgga gagaaccttt
     7621 agttcacata cttaaaacaa gaaggtacgg ctggatatgg ccgtgccttt tcttgataaa
     7681 atcatttaga aattaagaaa aactgtaaaa taagctgaaa aatgaaagaa agcgtcagtt
     7741 ctttcaaaca aaactttagt tcccttactt ttttttgtat aatgaaactt gatgaaatga
     7801 gggataataa atggcacaaa agaaacggcc tactaaaaaa aggaaaaaga cgaaaaaaac
     7861 gaaaaagcaa aaacagcagc aggaacaaat gctgacgatt ggtgttgggt ttgccttgat
     7921 tttatttgct gtcttcggct ttttaaaatt aggtttttta ggaatattga ttgcaaacgg
     7981 ctttcggatc attgcaggaa atacttacca aatcctctgt cttttattag ccgtattagg
     8041 attttggatt gtcattaaga atacagagtt ttcaattggt aaaaaccgac gctggtttgg
     8101 cggcatactc ttttatttcg gtatcttatt attgttacat gcccatttat ttggcaaact
     8161 ccatactggg gaaccaaata tcatggggac gacatgggat ttgttagcca gtgatataaa
     8221 acaaagtcaa gtagacaata acgttggtgg tgggatgatt ggagcaatcc tttatcattt
     8281 tacttacttc ttgattgcac agcctggaag ttatttagca gcagtgctat tactggcagg
     8341 aggagcattc ctgttcagta atctggaagg ctatcagctg ttaaatggta tccaatcaat
     8401 tggagaacga gtacaggaat tattggaagg tgatcctgcc aagcaagctc gaaagcaagc
     8461 ggcaaaggaa gagagaatga aacagcgcgc agaagcgaaa gaagcaaggc gcttagctgc
     8521 aaaagaagct gcagaaaaag aagctgcaga atatgagaag aaaaaagcgg tgagccagca
     8581 aagggaaaat actgctgatg aatggcagga accagaaaat acagagccgg agcagctaag
     8641 ttttgttcca atcgacagtt ttcaagaaaa tatccatcct gcgaatctag agaaaccagt
     8701 accagataca ccaaaacaga ccaatacagc agaaggtttt tcagatgagt tgccagagga
     8761 tgacggtacc tcattagaat ttgagattga agcagaacag gaaaatcaag actatgaatt
     8821 accgacagtt gatttgcttg attctatacc aactgtggat caaagtgatg agtataaaaa
     8881 aattgaaaaa aatatcggcg tattagaaca aactttccaa agtttcggtg tcgatgccaa
     8941 ggtcgtcaaa gccagcttag gaccatccgt cacaaaattc gaagtccagc cggctgtagg
     9001 ggtaaaagta agtaaaattg tgaacttaac agatgatatc gcgcttgctt tagcggcgaa
     9061 agatgtccgg atggaagcac cgattccagg taaatcactg attggtattg aagtaccaaa
     9121 tggaaagatc agtatggttt cctttagaga aatcatcgaa gcacaaccaa accacccaga
     9181 taaattacta gaagtacctt taggacgtga tgtttctgga cgggtacaaa cggctgatct
     9241 ttcgaagatg cctcacttac tcgtggctgg ttcgactggt agtgggaagt cagtagcaat
     9301 taatgggatt atcacaagta tattaatgcg tgctaagccc catgaagtaa aactgatgat
     9361 gatcgatccg aaaatggttg aattgaatat gtacaacggc atacctcatc tgttgacgcc
     9421 ggtcgtgacg aaccctagaa aagcagcgca agccttacaa aaagtagtac aagaaatgga
     9481 agaacgatat gaaaaattcg cggcaaccgg agtaagaaat atttctggtt acaatgaatt
     9541 tgttcaacag aaaaacttgg aaaacggaac gaaacatcca accttaccat tcatcgtagt
     9601 gatcgtagat gaattagctg atttgatgat ggtagccagc aatgaagttg aagatgcgat
     9661 tattcgttta gcgcaaatgg ctcgggcagc ggggattcat atgatcctag ctactcaacg
     9721 tccaagtgtg gatgttatca caggaatcat caaagcaaat gtaccttcca gaatggcatt
     9781 tgcggtatca agcggtacag attctcggac gatcattgat tctaatgggg cagaaaagct
     9841 cttagggcga ggagatatgc tattcttgcc aatgggtgag aataaaccga tccgtgtgca
     9901 aggagcattc atttctgacc acgaagtaga acgggtcgtt caatttgtta cagatcagca
     9961 agaagctcat tatgaagaga aaatgatgcc gacagatgaa gttgaaacga caggtgctcc
    10021 agaacagcca caagatgagc tgtttgaaga agcaaaagca cttgtcgttg aaatgcaaac
    10081 ggcaagtatt tcccttctcc aaagaagatt tcggatcggt tacaaccgag cagcacgttt
    10141 agtagatgaa ctggaagctc atggagttgt tggcccgtca gaaggaagca agcctcggaa
    10201 agtattcatt gagcaggaag aaacatcaga aacgttgccg gacgataatg acatgtaaaa
    10261 cttgtgtgat gaagggttga aaaacgaata accaataaaa agacaaggca tgatgatacc
    10321 aaaagaaaaa atggtttttt tccatctttt ctaatacggt atgatgtctt gtctttttag
    10381 tttttgtgag attaaagttc tattcgattt atttgtgaag tattgcatta aggaaatgaa
    10441 tcatgtatag tatgtagcga aactttgaga aaaagaggga aaatactttg gaaaaacagt
    10501 ccatttatgg cttgacgaat gaagaattaa tcaattggtt tatagaaaac ggcgagaaaa
    10561 aattccgagc tgcccaagtt tgggaatggt tgtatcaaaa acgtgtaagt aatttcacag
    10621 aaatgacgaa tctatcgaaa caattgatcg aaaaattatc ggagcatttt atcatcaatc
    10681 ctctaaaaca gatggtcgtc caagaagctt cggatggtac agtaaaatat ttgtttgaac
    10741 tccctgataa aaacatgatt gagactgtac tgatgagaca agaatatggc ttgtcagttt
    10801 gtgtgacgac acaagtagga tgtaatatcg gctgtacgtt ttgtgcaagc gggctgctca
    10861 aaaagaaccg tgatctgact gctggagaaa ttgttgcaca gatcatgatg gtccaacatt
    10921 attttgatga gcgcaatctt ggcgaacgcg tttcacatgt cgttgtaatg gggatcggtg
    10981 agccatttga taattacgac aatgtaatgg actttttgca tatcatcaat gatgccaaag
    11041 gattggcaat tggtgctcgg cacattacag tttccactag tggattagca cacaaaatca
    11101 aggaattcgc taataacggc ttgcaagtca atctggctat ttccttgcac gcgccgaata
    11161 atgaggtgcg aacatcgatc atgcggatca accgaagttt cccaattgag aaattgatgg
    11221 aagctgtgga tgagtattta gagaaaacaa accgtcgaat cacgtttgaa tatatcatgt
    11281 tgaatcaagt aaatgataga ccagagcatg cgcaacaatt agcagatcta ttaaaagata
    11341 agaaaaaatt agcatatgtc aacttgattc catataatcc agtcagtgaa catgatcaat
    11401 attctcgcag tccaaaagct gatgtattga gattttatga tgtattgaag aaaaacggcg
    11461 taaactgtgt gatacgtaaa gaacatggta cagatattga tgcagcttgc ggacaattgc
    11521 gaagcaagca aatgaaaaaa acagttgcca aataatcgta tcctactcgt tttttcaatt
    11581 accataaaag gagcagtttt cttaattaag gaactgttca acttaaataa gagaaaagat
    11641 tctatagcga aagctataaa gctttttttg acaagaaaaa ccctcatcaa gcaaagaaat
    11701 gtcctcaatt tgatgacagc ctgcttgatg ggggttttag caattattta actgattttc
    11761 ttatatcctt gctggctttc ttgtgtagca tgataagttt ctacgtcaag aatggcctgt
    11821 ttaaattttt ctcgggtgat ttcataaggc acatgtttaa tatcatttgt tgccatggct
    11881 ttatctaaga ttgcttctaa atcggtcgtg acattgagat tcatgtcggt taacgatact
    11941 ggaagttggt tctttttcat aaacacatag attttatcaa acaaatcgaa ctgctgatcc
    12001 atcatcaata aggtcagtac accataactg acagatgctc cgtgaaggta tccctccatt
    12061 ttttctagaa cagtcgtacc gtaatagaat gagtgtgcca tagcagaatt atagttattg
    12121 ttaactaaaa cagaaaccaa tccggtagta acaatgatat ccgaaacgac ttgttcaatt
    12181 gcatatgagg gacgatcgtc ggcactatct ttcaatgctt gcgtaccata agtcaagaga
    12241 cgatcaatgc acatttttcc gatttggatt cctaatctat ccgtgtagcc tagatcatcg
    12301 ccacgagcag aaaaagtaga ttcgacttct ttggataaag catcgccaat acctgcccat
    12361 aagtatttat caggtgcttc ggcaataatt gctgtatcaa tgaaggtgtg tacaggtggt
    12421 tttgaaggaa agaagagtcc gtccattacg ccttcttcgt tatagatgac acataccgca
    12481 gtgacgggcg aacagttcga accaatagtg ggaaaggtga agactggttt agctagtttt
    12541 tcaccaatga cttttacagt atcgcaagca cggccaccac cgactgcaaa caggacgtct
    12601 gctttttgga cactttcatg ctggatcaac tgttcaatat ttccataagt tgaattaccc
    12661 ccgtaccaga gactggcgac aacctctatt tcctgtgtca aagctacttt tattttgttt
    12721 tctgcctttg aaagtgcagt cttccctcca atcagcacta cttttgtccc atattctttg
    12781 acgattccat ttgctttttc atacgcatct gttccaatgg tataatttgg taagaaaaca
    12841 ctttgactca tattcatttc cttctttctt cgttgaaacg ggttaaaatt ttaaagagtg
    12901 atcgatctga ctgcggctgt agatggagta gaggtgaacc taagacagga taagtgatga
    12961 ataaggtggc atgaacacct tgaaatcatc aataagtatc ctaaaaacga acaagtacaa
    13021 aaacattgaa aacgacaata atgaattttt gttcctctta gtatttctac cgattccctt
    13081 tgcttttttg taattatcta aaaattctaa aaattaagat tattatatga aaacaggttt
    13141 tatttgtcaa ttcataattg gtttttttta aagcaaaaga taatctatat catgatgaac
    13201 aaacagagtt ttttcctcca attgatggct ttcctgatct ttcctatttt gaataaagtg
    13261 tttctgtata atttttttat tttataagta agataaataa tgaaagaata gagatactga
    13321 ttggtagcta aagatttttg agcgagaaca tttcgttttt tcaaaaggac tgttagaata
    13381 agcaagaaca agggggaaga cgtatgataa tcagtgaatt tgaccgtaat aatccagtat
    13441 tgaaagatca gctttctgat ttactgagac tgacttggcc ggaagaatat ggagacagct
    13501 aggcagaaga agtagaagaa atgatgaatc cagaacgaat cgcggtagca gcggtagacc
    13561 aagatgagtt agtaggattt attggtgcaa tccctcaata cggtatcaca ggttgggaat
    13621 tgcatccatt agttgtagaa agctcccgac gaaagaacca aataggtact cgattagtca
    13681 attacttaga aaaagaagta gcttccagag gaggaatcac gatttattta ggtacggatg
    13741 atttagacca tggaacaacg ttaagtcaaa cggacctgta tgagcataca tttgataaag
    13801 tggcttccat ccagaacctt cgtgaacatc cgtatgaatt ctatgaaaaa ttaggttata
    13861 aaatcgtagg tgtcttacca aatgcaaatg gctgggacaa accggatatt tggatggcaa
    13921 aaacgattat tcctcgacca aattctcaat aataaaaaga ggttgtgaca gaagtaatca
    13981 gcttcaagaa ataagaagga atttacgaaa attacttctt gaatttttgt gaaattccaa
    14041 cttatttccg aagaagctac ttttggaaca ccgtgtacca gaggttgtga aaaagtcgct
    14101 tagtttcgag tagtaagaaa aattttgtga aaatggcttt ctacattttt gcataaattt
    14161 ggcttaatac cgagaaactg gcttttgaac accgtttatt cagataaggg actgtgacaa
    14221 gacttttgtc acagtttctt tttgtttatt cttttttatc tttgtatggc aataatagac
    14281 aatataacga aaattattta atggcttttg gaagaccatg gaaaaccttg tacaaagcta
    14341 ttttttctga aaaattcctt gaaacctcac gaaaaaatca ttattttatg aaaaagacaa
    14401 aaaaaagtgt tgatttttta ataattattc attaaactct taaacaactt cagaaaatat
    14461 taattattct gaaaattcca ataaaggggt acgtaaaatg aaaaagaaat ttacatttgg
    14521 tttggttgcg gtatgcggcc ttgtacttgc tggatgttat ggcggcagtt cagccacaag
    14581 cagtagttca gcaaaaagcg gttcagcaga tggaggaggc gtatttaact tagtcgtgcc
    14641 tcaagaaatg cctacagctg acttatcagt tgcaacagat acaatcagtt ttacagcatt
    14701 gaataacgtt tatgaaggaa tttaccgtct tgatgaagac agcaaaccac agccggcagg
    14761 agctagcgag ttggcagaag tcagtgaaga cggattaact tacaaaatca aattacgaga
    14821 agacgcaaaa tggtcaaatg gtgatcctgt tacagcagcg gattacgttt atggctggca
    14881 acgaacagta gacccagcta cagcttctga atatgcttac ctgtatgcgc cagttgaaaa
    14941 tgcagaagac attacagctg gaaagaaaga taaatcagaa ctagggatca aagcagttgg
    15001 tgactatgaa ttagaaatca aattgacgaa acaaacacca tatttccaat atctgcttgc
    15061 tttcccatca tttttcccac aaaaacaatc cgtagtggaa gaaaatgggg atgcctatgc
    15121 atctgcaagt gataaagctg tttataatgg accattcaca ttagaagatt ttgatggacc
    15181 aggaacagat acagaatgga cttataagaa aaatgatcat tactgggaca aagatacagt
    15241 aaaactttct gaaatcaaag tcagtgtagt aaaagaatct tctactgcat tgaacttatt
    15301 caaagatggc caagcagatg acgtgatctt gtctggtgag ttagcacagc aaaacgcaaa
    15361 cgatccagct tatacttctg ttaaagaagc gcggacaagc tatatcgaat tcaatcaacg
    15421 tgaaaagaat tcaccattca acaatgtgaa cctaagaaaa gctatttctt attctatcga
    15481 tcgtgaagct ttagtaaaac aagtactagg tgacggatca gttgtctcta caggattgat
    15541 cccatcagat atggtaaaaa gtccaacaac gaacgaagac tttgcaaaag aagctggaaa
    15601 attagtttct tatgacaaag ataaagcaaa agaatattgg gaaaaagcga aaaaagaatt
    15661 gggcgttgat tctttagaat ttgatttgat ggcttctgat gatgattcat cgaaaaaagt
    15721 aatcgaatat gttcaaaact caatccaaga aaacttggat ggcgtaacag tcaaaccaac
    15781 acctgttcca ttctctgtac gtcttgaccg ctcaacttcg ggtgattttg acgcagtact
    15841 aggcggatgg gctgctgact atgcagaccc aagcagcttt actgatttgt ttgtcacagg
    15901 taattcatac aaccgtggtc gctggagcaa tgcagaatat gacgaagcag ttaaagcagc
    15961 ttcatcaaca gacgctggcg atgaacaagc ccgttgggat gatttccaaa aagcaatcaa
    16021 gatcatctct gatgatatgg gtgtcgttcc ggtgtaccaa aaagcagaag gtcacctagt
    16081 gaatgaaaaa atcaaaggta tcgttcacca tgcggcaggc gcttcatggg attacaaatg
    16141 gacatacgtt gaagaataat cgttaatacc gtgagcatga gaaaaaacga ggctgcggca
    16201 aatgtcgcaa cctcgttctc atattcactt atcctaacaa agaaaaaagc atgaaaatag
    16261 cagaaagagc aattagtatt ccggcaatga tcagccaaaa gctgtacata ccgtagctgg
    16321 cagacgagag cgaggaaaat tctggttttt tctttcggtt ccttgttcgt gcaagatgga
    16381 ccgatcgctg aaaatgatcg aagaaacccg aagaaaagac ccataaaaaa ccgccgataa
    16441 tcagaaacgg caatgaccat aagaatagat cattagataa ttgaatgagt gtaaaggaat
    16501 ggttgatgag attttttatt aaaataacca gaatcagtag aaaagctact gcgtagggga
    16561 tagatttttt tttcatttga ttgctccttt atttttggtt cattatctaa taatgtagcg
    16621 aaacatctgt cagaagtcaa aagctagggg gaaacattag tgaatagtta tatcaaatat
    16681 gtattaaaac gtgtcttttt catgatcatc acgctttggt taattgcaac aatcaccttt
    16741 ttcttgatgc aattgctgcc gggcacgcca tatacgaatc aggaacgttt gagtcctgaa
    16801 acaatcgaga tgctaaataa acaagtcggt ttggataaac cagttatcgt acaatatggg
    16861 atttatcttt ctaatttgct tcaaggagat tttggaattt ccttccaatt taaaaaccag
    16921 ccagtcgcac atttgttagc tggaagaatc ggaccatcct tgcagttagg gttacaggca
    16981 attatctttg gaacagtcct agggacgatt ttaggaacga tctctgccat gaagcaaaat
    17041 acatgggcag atacatcttc tacactcgta gcgatattag gtcgttccat tccaaacttt
    17101 gtatttgctg ttttattaca atatatcttt gcaatcaaat tacaagtttt gccaatcgca
    17161 aaatgggatg gctttgtgta cacaatctta ccaacaattg cgttggccat gtcgccgctt
    17221 gctgactctg ctcggtttat ccgtacggaa atggtggaag tactgcatag tgattatgtg
    17281 gaattagcaa aagcaaaagg attgagccgt tgggaaatcg cctttaaaca tggtcttcga
    17341 aatagtttga ttccattgat gactttatta ggaccgttag ctgtagcatt gatgaccgga
    17401 tcactggtgg tagaaaatat ctttgctatt cctggtatcg gggaacaatt cgttaagtca
    17461 atcacaacaa atgattatcc tacgatcatg gctgtaacga tcctttactc atttatgctg
    17521 atttttgtta ttttgatcgt ggatctattg tacggtctag ttgatccaag aattcgtgtt
    17581 tcagaaggga gccgaggata aatggaaatg ataccaaagc gttatgaaac agttgcagat
    17641 atccctgcgg atgaatttct tccattacaa aaaaataccg aagaagaacg tgaacagatt
    17701 actgcacctt ctttgaattt tatccaagat tcatggcgcc gtttaaagaa aaacaaagct
    17761 gccgttattt caatggtttt actattggtc atcattttga tctctgtgat cacaatcttt
    17821 gtttctcctc atgatccaac tgcccaaaac gtggcttata tcaatttgcc tcctcgtatt
    17881 ccgggaatca atatcgatgg attgaatgga aaagcaatgg ttggtggtga acttgttgat
    17941 aaatatgctc aagcaaacgt cccttcaaac gtaaatttct ttttaggaac agacggtttg
    18001 ggacgtgatg tattaagccg cttatttatg ggaatgagaa tctcgttgct gatcgcattt
    18061 attgctgcat tactagatgt aacgattggt gtagcttacg ggttggtttc tggtttgtta
    18121 ggcggacgtg tcgataacgt gatgcaacgt tttttggaaa tcttatcagg aattccaaac
    18181 ctcgttgtca tgattttgat gttggtcgtt tttgaaccag gaattttttc aatcgtggct
    18241 gctatggcca tcacaaactg gataccaatg gcacgtatcg ttcgtgcaca aacaatgaaa
    18301 ttaaaagatc aagaatatgt tttagctgga ttgacattag gtgaatcaag aatgaaaatt
    18361 gcattcaaac atattttgcc gaatatctct agtgtcatta ttattcaaat gatgtttagt
    18421 attccatctg ctattttctt cgaagccttt ttaagcttta tcggtttagg tcttacgcct
    18481 ccgtcagctt cattaggaac gatgttgagc gaagggtaca agacattcct gtatctgcct
    18541 tatctattat ggattccggc agtaacttta tcggttatca tgattggctt taacttactt
    18601 gctgatggat tgcgtgatgc ctttgatcct aaaatgaaag agtgaagacg atgacgaaaa
    18661 ttttagaagt gaaagactta gaaatctctt ttgatacgta tgctggaaag gtacgagcga
    18721 ttcgcggtgt caattttcat ttgaataaag gagaaacatt ggcgatcgtt ggggaatctg
    18781 gaagtggaaa atcagtgacg accagaagca tcatgcgctt gctgtcaagc aacgccaata
    18841 tagatgctgg tcaaatcctc tttaaaggta acgatatcgt tggaaaatca gaaagagaga
    18901 tgcaaaaaat ccgtggtaaa gaaatagcga tgattttcca agatccaatg acctctttag
    18961 atccgacgat gcccattggg aagcaagtcg ctgagtccct tagaaagcat aataaagttt
    19021 caaaaaaaga gggacaaaaa gctgcattag aattgcttaa attagttgga atacccgaag
    19081 ctgagaaacg tatcaacagt tatccgcatc agttctcagg aggtcagcgt caacggatcg
    19141 tgatcgcgat tgcactggta tgttatccgg aaatcctgat tgctgatgaa cctacaacag
    19201 cgcttgatgt gactattcaa gcacagattt tggagctgct gaaagaaatt cagacaaaaa
    19261 tcgatacatc aattatcttt atcactcatg accttggtgt agtagcgaat gtcgctgatc
    19321 gtgtcgctgt catgtatgga ggaagaatcg ttgaagttgg aacatcggaa gaaattttct
    19381 acaacccgca acatccctat acttggggac tgcttggttc aatgccgaca ttagatagtg
    19441 cgaacgatcg attgtatgct attcctggtt ctccaccgga tttattgaac ccgccaaaag
    19501 gagacgcatt ctatccaaga aatgaattcg cgatgaaaat cgatgcagaa caagaaccac
    19561 cattttttga actatcaaaa acacacaagg cagcaacttg gctacttgct ccgcaggcac
    19621 ccaaagtgac accgccagaa gaaatccaac gtcgctgggc tatctttaaa gagaaacaaa
    19681 aacatactta cggtcaagga ggaattgctg atgccaaata aactacttga agttaaagga
    19741 ttgaaacagt atttcaatgt aggaaaaaag aatgaagtac atgctgtaaa tgacatcagc
    19801 tttcacattt atgaaggaga aacattcggt ttagttggag aatctggaag cggaaaatcc
    19861 actactggcc gtacgatcat tcgcttgaat gaacctactg atggtgagat attattcgac
    19921 ggtcaagata taacgaagat caaagataaa aaaggtttga cgaaattccg gcatgatgtt
    19981 cagatgatct ttcaagatcc atacgcttcg ttgaatcctc gtatgaaagt cagagatatc
    20041 attgccgaag gaattgatgt taatggacta gctaaatcat ctgaagagcg tgcaaaaaaa
    20101 gtcgatgatc tattgagaac tgttggattg aatccaagcc acggtactcg ttatcctcat
    20161 gaattttcag gtgggcaacg ccagcgtatt ggtattgcac gagcattggc agtaaaaccg
    20221 cgttttatca tttgtgatga accaatttct gcactagatg tgtcgatcca agcccaagtg
    20281 gtcaatttgt tgcaagatct ccaaaaagaa catcaattga cctatctatt tattgcacat
    20341 gatttgtcga tggttaaaca tattagtgac cgaatcggtg taatgcataa cggcttgtta
    20401 ttagagatgg gaacaagtga tgaaatctat aatcacggtg ttcatcctta tacagaaagt
    20461 ttgttatctg ctattcctct accagatcca gaccatgaga gaaaacgcag aagaatcaaa
    20521 tatcaacctg aaccagatga cggacaagta cgtcaacttc gtgaaatcgc accagaacat
    20581 tttgtctatg caactgagca ggaagtccca tattacgcaa aaaaactaaa acgacaaaag
    20641 gaatctcttc tggttgctaa ctaggaggga catagatgct aaaagctttt aaattccgta
    20701 tgtatccgac agaagaacaa aaacagcaat tgattcgtac ttttggatgt gctcgattta
    20761 cctataacca tttgctgaaa aagcggcaga aaagttggca acaaacagga gtagccaatt
    20821 tttcgctcac acccgctaca ttaaaaaaag aatacccatt cttaaaagaa gtagacagtc
    20881 ttgctttagc aaatgcacag cttaatttgg atcgggcttt tcgcaattat ttcaagggac
    20941 gtgccagctt cccaaagctt aaaacgaaaa aaagtatgtg gcaatcttat acgaccaaca
    21001 accaaacccg tacgatctat ctggaaaatg gctacttaaa gttaccaaaa caaaaagaac
    21061 taatcaagat aaatcagcat cggccagttg aaggatcgat tcgctccgct acgatttctg
    21121 ctcgatataa tgaagaattt tatgtcgcac tactttgtga tgtttcgcca gtaaaaaaag
    21181 aatctttagc taaatggatc gggatcgcct atcatccgaa gacattgatc caaacatctc
    21241 gacctctaga agtaacttta ccaaaatttc accagacaga agaaaagctg caacatgcac
    21301 agcggaaatt gaatgtgaaa gtccgctccg ctcaccatag aaagatcagg ttagatcagg
    21361 caagcaatta tcagaaacaa aagagaaaag taatggatct ttatctgaaa caaaagaacc
    21421 agcgagaaga ttatcttgaa caattatctg ggaaattggt caaacaatat gactacttat
    21481 ttgtagaatc ttttccaaaa gaagaggcac atgctgattt ttctattcac gactggcata
    21541 aattaataac aaaattgcgt tataagtccc aatggtataa taaaaaattt cttttgatca
    21601 ataccgacgg agcagaagaa agcaattccg taagaaagag ccaagtagta gaaaaaatgg
    21661 gcagacattc ggttatcaag ggataaaaaa tgaatgaaac tgataggagc gttagggtta
    21721 gccttgttaa taaaaagtct taatgacttt tgtttctagg aatttttctt ttgaagaaaa
    21781 gctcactaat tatgacaaac caataaaaca atagcagaaa aagttcgtac gagctttttc
    21841 tgctattgtt aattttttat tcagaaagtt tacgaataca tcaaaattct gaaacaaaaa
    21901 acgtaactca caaagagttg tttttgcgtt ttttcttgaa tgattaggtc tgaatctttt
    21961 gctcacaacc ttattcatgg tgccccggct tgagctgata gatttttgaa ggacggccga
    22021 ttcctacagg acgttcaccg atctcatcga aatattggag catagctttc ttaaaattgg
    22081 aatgatcgat cgttttataa tcgatcccta agaatttagc aaatactttt cgagcttccg
    22141 taatcgtgaa atctttacct aaaacttgta gaacttgcgg ctcgtgttcc attttattga
    22201 ctacacggtt gaatgctttc aagatgattt gactatggtc aaaagccaaa gtatctttgc
    22261 cagaagactc tccagtgact aaatcaagcg agatagccac ttctccgcta gataaatgga
    22321 tcttgttact atgacgttct agtgtgaacc agcgtacttc cttggcatca tctccggcga
    22381 ttagcggttc ttctccaata aaggctaagt agctgactgt gacgacccag cctcgcggat
    22441 ctcgattagg cgtactgaat gtatgcagtt gctcaatatt ttcttttgaa ataaccacac
    22501 cagtttcttc ctttgtttct cttaagacgc tttctccagt tgactcatcc ttttggacaa
    22561 agccaccagg caatgcccat gaatttcgat aaggatgccc ttttctttgg atcaacaaaa
    22621 ctttcagctg atcttcttct ttgttatagc acatcagtac gatatcgaca gtcaaagagg
    22681 gcttgtcata ttctggacgt tcctgttttt gataccagtt aagaaattct tcttcacttg
    22741 ctttatgttc ataatataat ttttcttctt ctttagaagc aaaacggttc atctgaccac
    22801 ctccattgac agtaaaagta ctttttacct tattgtagca gactctgaaa aaaaaccaag
    22861 gatttacttg aaaaatctag gttatcattt aaatatgaaa aataaatgga agtatgagat
    22921 tgaataaaaa gaaaagcaga taaaacggca atactttata ccgctcttca cgaatggcaa
    22981 agaagccaca aataacgata tatacgtcaa tgataaaacg caaaatccaa gctgctgtca
    23041 taaaactgtt caaatgccca tggaggttaa aataaccata catacgaata taaaaatagc
    23101 tgaatattaa atgtgctaat gaaagaaaag caatactaag tgtaaaaaaa agatgtttct
    23161 tctgcatgat tgttccagtc ccttactaag atagatagtc tttattatac cacgtgtgag
    23221 gggagaaata aaaagccgtt ttttagtaga tatgaaacaa aacaaaaacc cgcatttatt
    23281 caacctattc atccttttaa aggatagatt gaacgtagat agaagtcgtt tatccagtct
    23341 ttttcatttt gaaaaaaagc taacagcatg ctttttttat ggtatactag caaggaaacg
    23401 aattcgcatc atgcgataaa ggaggaagag ccccgtggca gaaaaggaaa ctttttatat
    23461 cactacgcca atctattatc caagcggcaa acttcatatc ggcaactcgt atactacgat
    23521 tgcatgtgac gcaattgctc gttataaacg gttgatgggc tttgatgtgt tttatctcac
    23581 tggagtggat gaacatggtc aaaaaatcga aacaaaagca gaagaactag gtgtaaaacc
    23641 gcaagaatat gtagataaaa tggcggctga tgtcaagaaa ttatggaaaa cgcttgatat
    23701 cagttatgat aaattcattc gcacgacaga tgattaccat aaagcagctg ttcaaaaaat
    23761 atttgatcgt ttattagaac agggagatat ttacttaggt gaatatgaag gctggtactc
    23821 tgtttctgac gaagaattct ttacagaaac acaacttgca gaagtgtaca aagatgacga
    23881 aggtaacgtg atcggtggta aagcgccaag tggacacgaa gtagaattgg taaaagaaga
    23941 atcatacttc ttccgcatga gcaaatacgc agaccgtcta ttggaatact atgaagaaca
    24001 tcccgaattc atccagccag aatctcgtaa aaacgaaatg atcaacaact ttatcaaacc
    24061 aggattagag gatcttgcag tttcacgaac aacttttact tggggagtac cagtcaagag
    24121 tgatccgaaa catgtggtgt atgtatggat cgatgcatta tccaattata ttactgcact
    24181 aggatatggc tccgaagatg aaagtctatt cgaaaaatac tggcctgcag atgttcagat
    24241 ggtcggtaaa gaaatcgttc gctttcatac gatctattgg ccaatcatgc tgatggccct
    24301 tgatttgcct ctacctaaaa aagtatttgg gcatggctgg ctattgatga aagacggaaa
    24361 aatgtctaaa tcaaaaggaa atgtcgttta tcctgaaatg cttgtggaac gttatggttt
    24421 agatgcactc cgttattatt tgcttcgtgc tgttccgttt gggtcagatg gtgtctttac
    24481 gccagaagat tttgtttccc gtgtcaatta tgatctggca aatgatctag ggaacttatt
    24541 gaatcgaacg attgctatga tcaacaaata ttgtgatgga ctcgttcctg actatgcctc
    24601 acttgtcact ccatttgata gtgaactgtc tacgacggct gctaacgttg tcggacgtta
    24661 ccatgatgca atggaaaaaa tggaattcaa tacagcattg gctgaaatct gggtgctgat
    24721 ttctcgtgca aacaaataca ttgatgaaac agaaccatgg gtattagcaa aagacgaaga
    24781 aaagaaagct gaattgaata gtgtcatgat ccatttagct gaatctttgc gtatcgcagc
    24841 gatattactt cagccaatca tgacagaaac gccagagaag atcttcaatc aactaggatt
    24901 ggactctgaa acaatgaatc ttgaaggatt acactttggc gaatttcctt caggaacaaa
    24961 agtagttgca aaaggtacac caatcttccc acgtttagat atggaagaag aagttgcttt
    25021 tattcaagaa aaaatgtctg aaggtactca aacaaacgaa gatacagtca aatgggatcc
    25081 agaagaaaca gaacttgttt caacgaaaga aaaacaaatc aaatttgatg tatttgaaaa
    25141 agtagaacta aaagtagcag aagtcatcaa ctgtcaaaaa gtagaagggg cagataagtt
    25201 gctgcaattc cgactagatg ctggagacag ccaagaccgt caaatcttgt caggaatcgc
    25261 tgaattttat cctgatccaa gcgaattgat cgggaaaaaa gtcgtcattg tagcaaatct
    25321 gaaaccacga aagatgcgtg gacaaatcag tcaaggaatg atcctttctg cagaagcacc
    25381 agatggctct ttacaagtaa tcgaagcacc aaaatccatg ccaaatggct cagaaatcgc
    25441 atagaaattc aaagggggta tgacactagt tgtgtcatac cccctttttc cgaaatttga
    25501 gaagcaattt tcgtaaattc cttcttatta cttgaagcca atcatataat acagaaacag
    25561 agaactatcc aaaataaatc ctagaaaaag aagtacgagc ttcgctgatt tttgttaatt
    25621 tagaataata ctaataggaa tcgtaaaaac agaaaaaaag cataatgata aagggtcggg
    25681 cagattttaa ttgattcatc tggtactctt ttttattgtc cttacattca taaagtttga
    25741 atgcaatgac tttattacat ctatatttat atctaacagt agcgttgatg tgttaattat
    25801 aagttctatc actttatgtt caattagttt gtgctcttat atgtgtgatt gaaaatatag
    25861 ataaaaaaac aatcatcggc tttgacttcg atgattgttt tctttatttt gtaaaaatcc
    25921 tatcacttga ttttatggta accttttatt tttgtcttca aacggcttgg taagagagca
    25981 gtaagcagtg caaatgggat cgaaaagaca taccagaaaa tcgtacgtcc gataccataa
    26041 tgttgataga gctttgtcaa catcacgtgt aacaagatca accctaaaag caaggagcca
    26101 gctataaacc aatgattatc ccaatgcatg acttcactaa gtgccatcac tccaaaagga
    26161 tagaaataaa aatagatccc gactaaggtc atccgccagt tctttgtaaa tgttccgata
    26221 ttcaataaaa agaaaatcgc tgaaagcaaa ataccgatag gcggaaaaag cccaaccaaa
    26281 aaggaataca acatccccca caagatcatc acaggacctt tgatcaatgc aacgattgcg
    26341 atgaacaaag tcagcaatat tagtttttgt gataacgttg caacagtcat taatccaaat
    26401 aaaattaatg tgaaccaatg ccaatgggac tgctgttgtt ctttacggga aaaatcgacc
    26461 cggttggata aataatctgc taatttcgga taagaacgct taaaatccat aaactgttcc
    26521 tccttcgtgg aaaaggtagg tttagtatac gaaaaataaa aaagaaatac atccgtctta
    26581 agagggagtt tttcactgaa agtggagaaa aaagaatggt aaattaaaat attctcagtt
    26641 atactgaaaa ttataccaaa tttcaaagaa gaaagaggat gggaatgaaa ggcaaactaa
    26701 aacgagaaat caatctgttt ggcgcattag ctaccgttat gggaaccgtg atcggtgcag
    26761 gcgtcttctt caaaactgct gctgttactg ctagtactca gtctacaagt ttgacattgc
    26821 tagcttggct tttgggtggt ttcttaacga tctgtgctgg attgacagtg gctgaattag
    26881 ctacagcaat cccggaaact ggtggagctg taaaatatat tgaagctgcg tatggaaaat
    26941 tgcccagctt tttattagga tgggcgcaaa gcttgattta ctttcctgct aatattgctg
    27001 cattatcgat tatctttgcg acacaattga cgaacttatt acaattatct accgattatc
    27061 ttttgttgat tgcagtgatc accgctgttt ctgtgacagg gttgaatttg ttagggacaa
    27121 aagtcggtgc ttctgtacaa tcagtgacgt tgattgttaa attaatacca attgctgtga
    27181 ttgtcatatg gggattattg accccaggac aaggaactgt ccagttattt ccaatcgaag
    27241 ctggaaaaga tgttactttt gtcgaaggat tgagcagtgc gttgttagca acgttgtttg
    27301 cttatgacgg ctggttaggt gtcggtgcaa tggcagggga aatgaaacgg ccggaaaaag
    27361 atttgcctaa agcaattatt cttggtttga gttttgtaac agtcgtttat ctattgatca
    27421 attttgtttt tttgaaaact ttgccgattg atcatcttgc cggtaatttg aatgcagctt
    27481 ctgaagcatc agacgtcatt tttggaggaa tcggtggaaa gctggtcact attgggatct
    27541 tgatctcggt atacggtgca ttgaacggtt atacactaac tgggatccgc gtgccttatg
    27601 cgatggcttt agaagacgag ctacctttca gtaaacagct gacaaagctt tcaaagaagt
    27661 ttacggttcc ttatgttcct gcggttttcc agttagcagt agcttgtatt atgatgagcc
    27721 ttggttcttt cgacttttta acagacatgt tgatttttgt gatgtggcta ttcagtttac
    27781 tgatttttat cggtgtcttt attttaagaa aaaaagcgcc ggaattgcca cgtccatata
    27841 aagtaccact ttatccaatc gttccgatta ttgcgattct cggtgctatt ttcattttag
    27901 gtatgacgat gatgacgcaa acgaaattag cattgatcgg tataggtgtt accttgatag
    27961 gtatcccagt ctattatcaa aaaaagaaaa aacgctcaga agagtaaaat atttcaacaa
    28021 cagacagaaa aagtacgaac taggtcctct agatcttcgt gctttttcta tttcaataaa
    28081 acaagttctt ctattttgat ttttgctact tctgcaagta gtgcttatgt gctaaagtaa
    28141 gaatgaaaag gagagataaa catgatattt gattctcata cccatttaaa tgctgaacag
    28201 ttcaatgaag atattccaga aacgattcag cgagcacaag aaatgggggt aacgaaaatg
    28261 gcggtcgtgg gcttcgatac gccaacgatt gaaaagtctt tgcaattaag ccatgactat
    28321 ccaaatatat acagtattat cggctggcat ccgacagagg ctggcagtta tacgaaagaa
    28381 atcgaaaata aattacaaga acaattgact ttaccaaagg ttgtagcatt aggggaaatt
    28441 ggcttggatt atcattggat ggaagacccg aaagaagtcc aagataaagt atttcgcaga
    28501 cagattgcta tcgcaaaaga aatgaaccta ccaatcagta ttcatactag agaagcactt
    28561 gaagacacgt atcaaatcct aaaagaagaa gatgttcgag atatcggtgg aatcatgcat
    28621 agtttcagtg gtgattatga atgggcaaaa cgatttcttg acttagggat gcatatttca
    28681 tttagcggtg tcgtcacttt caaaaaagca ttagacgtcc aagaggctgc agcaaatgta
    28741 ccaatcgaac gattacttgt ggaaacagat gccccttatc tggcaccagt tccttttaga
    28801 ggaaagcgaa atgaaccagg ctatactcgt tatacagtag aaaagattgc tgaacttcgg
    28861 aatcttccga cagaagaagt ggctgcccaa acatggacaa atgcgcaccg attatttggt
    28921 ctaagcgaac atggataaga tgaagatcca agagattata gtcgttgaag gaaaagacga
    28981 tacaagaagg ctgcgggaag tagttgatgc agatacgata gaaacaatag ggtctgcaat
    29041 caatgaagag atcctgatgc aaattgaaca tgcccaagag actcgtggag tgatcgtttt
    29101 taccgatcct gatttttcag gagaaaaaat aagaaaaatc attatggaag ctgttcctca
    29161 ggcaaaacat gcttttttgc cgcgcacaca agctaaacca aagaaaaagg gaagccttgg
    29221 tgtcgagcat gcaagtgatg ctgctatttt agaagcattg aaaaaggtcg tgacgccaga
    29281 catttcccaa acggctgttt ctgaaatcac tagacaagac cttttagact atggtttaat
    29341 agctggggca caagcaaaag aaaaacgaga aaaactaggt gacgagttgc gcatcggtta
    29401 cacgaacggc aaacaattgg aaaaacgttt gaagatgttt cgtattacgc cgaaagaact
    29461 cgcccaagca atgaaaatag tggaggaaca acatgagtga atatcgtgaa attgcgacac
    29521 cctctagaac aaaagagatt ttattgaaac atggtttttc atttaaaaaa agtttaggac
    29581 aaaattttct gactgaacca aatattttaa ggaaaatcgt agaaactgcg caaatagatg
    29641 aacatacgaa cgtgatcgaa gttggaccag gaatcggtgc attgacggaa caattagcca
    29701 agcatgcaaa acaagtcgta gcatttgaga tcgatgatcg tttgatccct gtattggctg
    29761 atacgatgca accctatgac aatgtgacta ttgtacatca agatatttta aagaccgatt
    29821 tgtcaacagc tgttagagaa acatttcatg aggagcttcc gttaaaagtc gtagcgaatt
    29881 taccttacta tattacgaca ccgatcatga tgcattttct tgaatcagat ttggttgtgg
    29941 atgaactagt cgtcatgatg caaaaagaag tagctgatcg tatctctgct gaaccaggaa
    30001 cgaaagctta tggttcatta tcgattgctg ttcaatatta tatggaagcc agtttagcat
    30061 tcatcgtacc aaaaacagta tttgtccctc agccaaatgt agattcagcg attttgaaac
    30121 tgacaagaag agatataccg gcagtcgaag tgacagacga aaaagcattt ttccgattga
    30181 caaaagcggc atttcagcaa agaagaaaaa cgctttggaa taacttgcag cattcgtatg
    30241 gcaaagatga ccagacaaaa gcatggttag caaaaagctt ggaaacagct ggaatcgatc
    30301 caaaacgtcg tggggagact ttgtcattac aagaattcgc cgcattaagc aatgcgatgt
    30361 cagaaaatat gcagtaaaaa aagaggttgt gacagaagtt gtgaaaaagc tgtcctgcat
    30421 caagtaataa gaacagccaa acaaaaatag ttcctcatgt ttttcgtttg tttgggctta
    30481 ttaccgcaga tgcaagcttt tgaacaccgt ttaagaaaga ggttgtgggc ctgccgttta
    30541 actctaagtc acaaggaaca atttcaaaaa acagcttctc atgtttttga gttagttggt
    30601 cttattactt gatgcaagct tttgaccact attatgccga acaaggagat gtgacacaaa
    30661 ttttgtcaca tctcctattt ttgtttcttg atcaaaaaat tcttatactt gatactcaca
    30721 ctcattttgt atggatttag aaaagaatat gtgtcttgac agttattagt aagtgagtta
    30781 tactaagtcg acatttatgt gtcaagacat attaggagag agaaaaaatg agaaaaaatt
    30841 cagttcgaaa actaacgatt tctgctttat tgattgggat gggggtcatt atcccaatgg
    30901 tcatgcctaa aatcatgatt ccaccagctt cctttacttt ggcgtctcat gtgccattat
    30961 ttattgcgat gttttttaca ccaggagttg cagtggctgt tgcgctaggt acgacatttg
    31021 gtttcttttt aacaacgccg gttatcatcg cattgcgagc attgtcacat cttgtgtttg
    31081 cgttgattgg ggcttggtac ttgcaaaagc atccgagtat cgttttaaaa aatggacagt
    31141 ttacttttgc taattggcgt ttccaagggt tcaattttgt gatcggtgtg attcattctt
    31201 tagcagaaat gctagtagtc agcatctttt actttattgg taatatgcct gaaacatatt
    31261 attctcaagg atatttttat acagtattca tcttgatggg gattggtggg cttatccaca
    31321 gtttagttga ttataatatt gcttatttta tagcaggaac actcagcaaa tattttgata
    31381 tccccgtatt tacggcagca aaaaaagaaa atcggaaaga gtcgattcaa gggtacgcga
    31441 agaaaacgac atattagaca tgatctctca aaaaaaaaac tctcatctag taatggataa
    31501 taaatcctct aagatgagag ttttttgtcc taaaaagagc tgcaccaaca tcttctggca
    31561 cagctctaac agggggcaat ttatggaagt ttgatgactt gttctgtttc tttatcaaag
    31621 aagtggcctt tgctgatgtt gaaggcaagg tcgactattt cgcctggttt atggaaatct
    31681 cttgcatcta cttttgagat gaactcagta gaaccagttt ttgtgtaaag catcgtttca
    31741 gctccaagta attcggaaac gacaacttct gatttcacta cggcttcagg tgaagcttca
    31801 agggctactt gttcactgtg gatgtcttct ggacgaatac cgaagataac ttcttttcct
    31861 tcataacctt tttcaactaa tactttgttt tttccttcag gaatacgaag cttaagccca
    31921 tgtccatctg tgataacacc atttttcaat gtcacattaa agaagttcat ggcaggagat
    31981 ccaataaagc cggcaacgaa catattgacg ggtgtatcat aaacttcttt cggtgtaccg
    32041 atttgttgga taaatccgtc tttcatgata acgattcggt cagccatagt cattgcttct
    32101 gtctgatcgt gagtaacata gatggtcgtt gtttcaagac gctggtgaag tttggcaatc
    32161 tcagcacgca tggctacacg taatttagca tctaaatttg ataatggctc atccattaag
    32221 aagacttttg catcccgaac gattgcccgt cccaatgcca cacgttggcg ttgtccacca
    32281 gataaagcag ctggttttcg tttcaagtat tctgtcagac ctaagatctc tgctgcattg
    32341 tccacacgtt ttttgatgtc tgctttatca tatttacgta atttcaagcc aaaagccata
    32401 ttatcaaaaa cagtcatatg aggatacaat gcgtagtttt ggaaaaccat ggcaatatca
    32461 cggtctttag gtgccacgtc attcatgact tttccgccga tatttagttc tccttcagag
    32521 atatcttcca gtcccgcgat cattcgcaaa gtagttgact ttccacaacc ggaaggacca
    32581 acaaaaacga taaattcacg gtctttgatt gatagattaa aatcagttac agaataattt
    32641 tctgcattgt catatttttt atatacgttt ttcagagcca tttctaccat aatacgttcc
    32701 acactttctt ttttagtttt attaactatt taaagtataa atgaaagcgt taactaactt
    32761 caatgtcatg ttgaacaaga attttttctt ttttttgtca atgtgctagt tttaagcttt
    32821 aataaatatc cataagaaat aaaaactgat acactggagt aaataagtat gttaacaata
    32881 tcaaaaggag tgtctcttat gaaaatcgct ttgatcgctc atgaccgaaa aaaacctatg
    32941 atggtgaaat tagcaacagc gtataaaaca atattggaaa aacatgaact ttttgctacc
    33001 gggacgactg ggcagaggat cacggaagca accggcttgc cgatccatcg gtttaaatcc
    33061 ggaccgttag gcggggatca gcaaattgga gccatgattt cggaggatca gttagatttg
    33121 gtgatttttc ttcgagaccc gctggctgca cagccccatg aaccagatgt aacagcactt
    33181 atccgtttat ctgatgtcta tgagattccg ttagctacga atatcggaac agctgaggtg
    33241 ctactaagag gactggaagc cggcttccta gattttcgaa gaatcgtcca tgaaatagac
    33301 aagcatccac tttcattttg atggataagc gtatggaaaa ataacgttta atgttcgcct
    33361 atatgtagct ataaaagcta aacaaactga taatatgcga tataatacga acgtattgga
    33421 taatataaac aataatatta gtttttcgta tttgcagtaa atgtgaaaat atgctatagt
    33481 ttctgtggga aaagatccca aaaataatac agagtagaaa ataaagcgtt aagtgctaag
    33541 aggatgggat gttgcctctt ggacgaagga cgtaatgttc gcggttttat tttcgcattc
    33601 gctgcatgat acgatgtagc agctcttaaa ggagatgcta aaaatgaaga aacaccggtt
    33661 agacgcacgt atgattgcta ttatgggctt attgattgca ttgatggtca ctttgtcccg
    33721 actggtagca atcgagaccc catttattaa aatcagtgtc acatttattc cacaagtgat
    33781 catgggcatt ctttttggtc ctttttggtc agggataggc gcagttttag ctgatttagt
    33841 aggaatggcc ctcttctcga aatctgcatt ctttattgga tttacattga atgcatttat
    33901 tgaaggagca atctacggat tctttttcta tcgaaaagag atcacttgga aaaatgccat
    33961 tctggctacg cttagtgtca cgctgatcat caacttattt ttgacaccat tatggcttgc
    34021 gttgatgtac catgtgccat tgttcagctg ggtcgtttgg gcgccccgcc tgttaaaaac
    34081 agtaatatgg ttaccgattc aatcaattgc catttattac gtaggacgta gtattcctta
    34141 caaaaaaatt ttaagaagtt tagcgatcca cgctaaataa aaaacggctc tttgtcaaat
    34201 gatgttggta aagagaaatc ttagaataat ttggacagaa cagaaagaag aaaacctctt
    34261 tctgttctgt ttatttggtt aaaaatgttt tgccatgacg aatcataatt cgtagtttga
    34321 aattgtaaaa gttgcgaaag ccgtacgcat tgcgttttaa tactttgatg tggttgttta
    34381 agcattccaa tggtccgttt gaatagctgt agtgaagcgc attttggatc tgtggcagaa
    34441 actttttaaa cgttcgtaaa gtggttgtat aggtttctgg cagaccgcta atgtctctag
    34501 ttaaacaatc ctcaaatagt gactgatctc ttttctttac cgcataaaga atgtcttggt
    34561 ataactgata gccttggcgt aagttgggac aagcagctaa cagtcgatcg gcaatctcac
    34621 tttcagtcaa ataatcttta aaactggcac gccaaacccg tttttcatag tttaactttg
    34681 cttcttcttt ttgtaggagt ttccaatagc gcttcaattt tttccctaac cctcgttgag
    34741 agtgggaatt tcctttaagt cgtaccgtgg tttctttcac tcgatgattc aagaaagttc
    34801 tgccaatatg ttggacgatg tggaaacgat cgatgatcaa ctgagcattc gggaaaagct
    34861 ttttcaccaa cgaaacataa ggcgcataca tatcaataac aatcaattga acggcttcac
    34921 gagtcgctaa tgaaaaccga gaaaaatagc gttctaaata aggcaagcgt cgattttcga
    34981 caatatccaa taatgcatgg ctttgtccgt ccatcatgat aaaactcatt gcactggcaa
    35041 cacttcgaac cgatttgaat tcatcaaagc ataagacctt tggcaaagtc tgtttgaaag
    35101 gattcaatgg gcggtagaat cgcttcatga cacgatagac cgaagaagtg gaaatatttt
    35161 tctgacgagc gatttccgtc atggaagtat tctcttttaa gcgagcaatg acagaccaac
    35221 gaactttttg actaatgaaa cagtgttttt cagcaacaga ggtatccgct acgaatgttc
    35281 tttggcaaga cttacattta aatcgttgtt tcttcagctc cagataagtg cgatactcag
    35341 acacatcatt cagtaacaac cgaacggttg tcgtccccca acgaatgatc ctagagggga
    35401 cgacacaaag acaatgggaa cactcttctg gcttgtacag aagagttcct ttgtaaatca
    35461 agcaaatttg ccctttgtat ttttctttag ttaaacaatt ttcctcgaaa tagatattta
    35521 ggtctagaat atttagggta tctttgataa gctgagtatg agacatcttg aatcctccta
    35581 tgaattggta gccaaattaa ttctaaagga tttcagggtg tcttttctat atttttttat
    35641 acaaaaaaga tg
//
LOCUS       DACDIH010000022        35264 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDIH010000022 DACDIH010000000
VERSION     DACDIH010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.022"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(224..1072)
                     /locus_tag="IV281_001163"
     CDS             complement(224..1072)
                     /locus_tag="IV281_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8780189.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1296..3236)
                     /gene="thrS"
                     /locus_tag="IV281_001164"
     CDS             complement(1296..3236)
                     /gene="thrS"
                     /locus_tag="IV281_001164"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP8780190.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3573..3752)
                     /locus_tag="IV281_001165"
     CDS             complement(3573..3752)
                     /locus_tag="IV281_001165"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP8780191.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3799..5622)
                     /gene="cadA"
                     /locus_tag="IV281_001166"
     CDS             complement(3799..5622)
                     /gene="cadA"
                     /locus_tag="IV281_001166"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8780192.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5900..8179
                     /locus_tag="IV281_001167"
     CDS             5900..8179
                     /locus_tag="IV281_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8780193.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8272..8919)
                     /locus_tag="IV281_001168"
     CDS             complement(8272..8919)
                     /locus_tag="IV281_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780194.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9098..9817)
                     /locus_tag="IV281_001169"
     CDS             complement(9098..9817)
                     /locus_tag="IV281_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8780195.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9948..11099)
                     /locus_tag="IV281_001170"
     CDS             complement(9948..11099)
                     /locus_tag="IV281_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP8780196.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11316..12056)
                     /locus_tag="IV281_001171"
     CDS             complement(11316..12056)
                     /locus_tag="IV281_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8780197.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(12060..12383)
                     /gene="rsfS"
                     /locus_tag="IV281_001172"
     CDS             complement(12060..12383)
                     /gene="rsfS"
                     /locus_tag="IV281_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP8780198.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12440..13033)
                     /locus_tag="IV281_001173"
     CDS             complement(12440..13033)
                     /locus_tag="IV281_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8780199.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(13017..13664)
                     /locus_tag="IV281_001174"
     CDS             complement(13017..13664)
                     /locus_tag="IV281_001174"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP8780200.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13677..13988)
                     /gene="yhbY"
                     /locus_tag="IV281_001175"
     CDS             complement(13677..13988)
                     /gene="yhbY"
                     /locus_tag="IV281_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP8780201.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(14004..15113)
                     /gene="yqeH"
                     /locus_tag="IV281_001176"
     CDS             complement(14004..15113)
                     /gene="yqeH"
                     /locus_tag="IV281_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP8780202.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15110..15637)
                     /locus_tag="IV281_001177"
     CDS             complement(15110..15637)
                     /locus_tag="IV281_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP8780203.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15817..16602)
                     /locus_tag="IV281_001178"
     CDS             complement(15817..16602)
                     /locus_tag="IV281_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP8780204.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16595..17464)
                     /locus_tag="IV281_001179"
     CDS             complement(16595..17464)
                     /locus_tag="IV281_001179"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP8780205.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17465..18844)
                     /locus_tag="IV281_001180"
     CDS             complement(17465..18844)
                     /locus_tag="IV281_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP8780206.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18852..19271)
                     /gene="fabZ"
                     /locus_tag="IV281_001181"
     CDS             complement(18852..19271)
                     /gene="fabZ"
                     /locus_tag="IV281_001181"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8780207.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19296..19772)
                     /gene="accB"
                     /locus_tag="IV281_001182"
     CDS             complement(19296..19772)
                     /gene="accB"
                     /locus_tag="IV281_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP8780208.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19775..21010)
                     /gene="fabF"
                     /locus_tag="IV281_001183"
     CDS             complement(19775..21010)
                     /gene="fabF"
                     /locus_tag="IV281_001183"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP8780209.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(21029..21766)
                     /gene="fabG"
                     /locus_tag="IV281_001184"
     CDS             complement(21029..21766)
                     /gene="fabG"
                     /locus_tag="IV281_001184"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP8780210.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21778..22695)
                     /gene="fabD"
                     /locus_tag="IV281_001185"
     CDS             complement(21778..22695)
                     /gene="fabD"
                     /locus_tag="IV281_001185"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8780211.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22802..23026)
                     /locus_tag="IV281_001186"
     CDS             complement(22802..23026)
                     /locus_tag="IV281_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8780212.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(23079..24041)
                     /locus_tag="IV281_001187"
     CDS             complement(23079..24041)
                     /locus_tag="IV281_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP8780213.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(24051..24491)
                     /locus_tag="IV281_001188"
     CDS             complement(24051..24491)
                     /locus_tag="IV281_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8780214.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24797..25762
                     /locus_tag="IV281_001189"
     CDS             24797..25762
                     /locus_tag="IV281_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8780215.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25819..26049)
                     /locus_tag="IV281_001190"
     CDS             complement(25819..26049)
                     /locus_tag="IV281_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP8780216.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26135..27022)
                     /locus_tag="IV281_001191"
     CDS             complement(26135..27022)
                     /locus_tag="IV281_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP8780217.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(27111..28334)
                     /locus_tag="IV281_001192"
     CDS             complement(27111..28334)
                     /locus_tag="IV281_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8780218.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28351..29430)
                     /locus_tag="IV281_001193"
     CDS             complement(28351..29430)
                     /locus_tag="IV281_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8780219.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(29561..31288)
                     /gene="ptsP"
                     /locus_tag="IV281_001194"
     CDS             complement(29561..31288)
                     /gene="ptsP"
                     /locus_tag="IV281_001194"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP8780220.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31288..31554)
                     /locus_tag="IV281_001195"
     CDS             complement(31288..31554)
                     /locus_tag="IV281_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP8780221.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            32033..34267
                     /locus_tag="IV281_001196"
     CDS             32033..34267
                     /locus_tag="IV281_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8780222.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34415..34735
                     /locus_tag="IV281_001197"
     CDS             34415..34735
                     /locus_tag="IV281_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8780223.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(34940..35164)
                     /locus_tag="IV281_001198"
     CDS             complement(34940..35164)
                     /locus_tag="IV281_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP8780224.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT         9946 a         7605 c         6120 g        11593 t
ORIGIN      
        1 tgggcggagc aagaaattct ttacctaatt tcaagataaa atcaactttt tgtttcacag
       61 caatcactta aagttacata tccaactatt tttttcatta aaaaataaac ttatttttga
      121 aaattatgtt ttcttaagtc gtgaaacact ttgatggatc atttatttgt ggaaaactac
      181 gttttgttat tctaaaaaac ttatataaaa aatagtaagg tttttatttc gaatccataa
      241 aatattcttg gattgcttct cgtgtttttt tcactttttc gctaagcttt tcgtttttag
      301 ccatatagaa ataaaaaatg atatcgatcg tagcaaattg ggccaaaagt gaattagttg
      361 ccgcacttcg attcgtgatt tcttttggtc tagatgtttg tacgagtaca tcggctatct
      421 tgctaagcgg gttatttcca aattgcgtca acgcaatgat ttcgatattc atgcttttcg
      481 ccagctcggc taatgcgact acatctgcac ttctacctga gtttgaaaca agccaaagca
      541 ctttcttttt ctcattgctg acaagttgcg ggagcaagac gtggatatct ttttcaaaaa
      601 tgattggctt accgacacga gaccattttt gcagaatatc ttctactact aaagaagagg
      661 cgccaacccc atataaaaaa acagtctctg ccgattccaa acactctacg actttttgga
      721 tcgtttcttc ttctagaata tctactgttt cttttatcgc tacttgtgca ttgttaccaa
      781 gtttgttttt gatagagtaa aaggactcat ttgcttctat atcactaaaa ccttcatcga
      841 ttccagtctg tagttctgtt gaaatcgtga ttttgaagtc cgtcagactt tgaaaaccga
      901 tttttttgga aaaacgtaca actgtcggcg cactcaaccc ggaagcttct gctaattcat
      961 tagctgtcat tgtagggacc ttctcaatat taccgataat gtagttggcc aacttttttt
     1021 ctgctgaaga ataatcttcc atcatacttt caatacgact aattacgctc ataagttcct
     1081 ccaccctttt aatgatagtc tatgtaagta taccatatca gacccttatt cacggtcgta
     1141 tactggataa aaaacaaaaa aacaatccgc ccaacagaat atcttatccc atcaatccca
     1201 cagattctat tgagcatctg tgtctttgag gaatacctgt tatctattgt tcggattgct
     1261 ttctatctac tactttttta caggaaattt acctattagt tttctctgct gtaatttttg
     1321 acttctgctt ccatagcatc aataaagata ttcaaatctt ctacagctgt ttctttactt
     1381 ccataacgac gtacgttcac tgttgcatct tccaattcct tgtctccaac aactaattgg
     1441 taagggatct tttgtgtttg agaggcacgt atcttatagc ccattttttc attacgatca
     1501 tcaacttcta cacgcaaccc cttcatttgc aaacgttctt tgatttcata agcataatcg
     1561 ccatgtgcat caacagaaac agggatgatt gttgcttgta taggtgccaa ccatgttgga
     1621 aaagcgcctt tgtatacttc tgttaaataa gcgacaaaac gttccattgt agaaacaatt
     1681 ccgcggtgga taactactgg acgatgcgta ttttccccgt cttctccaac atatgtcaaa
     1741 tcaaagcgct ctggcaataa gaagtctaat tgtatagttg ataatgtttc ttctgtacca
     1801 agagctgttt tgacttgaac atctagtttt ggtccgtaaa aggctgcctc gccttctgct
     1861 tcaaagtaat ctagttctaa ttcatccata gcggctttca gcattgcttg ggctttttcc
     1921 cacattgcgt catcatcaaa atatttatct gtattgtttg gatcgcgata gctcaagcgg
     1981 aaacgataat ctgtaatatt gaagtcagca tatactgcca ccatcaattc aagcgtacgt
     2041 ttaaattcat ctttgatttg atcaggacga acaaatgtat gaccatcatt caacgtcatt
     2101 tctcgtacac gttgtaaacc tgacaaagca cctgattttt catagcgatg catcattcct
     2161 aattcagcga tacgaattgg taattcacga taagaatgga tcgtgttttt gtagaccatc
     2221 atgtgatgag ggcagttcat tgggcgaaga acaagcattt cgccatctcc catatccatt
     2281 ggcgggaaca tgtcttcttg ataatgatcc cagtgtccag atgttttgta caactcaaca
     2341 tctcccatta ttggtgtata tacatgttgg taacctagac taacttcttt gtctacgata
     2401 taacgttcga tcgtgcgtcg gattgttgcc ccttttggaa gccagaatgg caaaccagaa
     2461 cctacttctg gggaaaccat gaacaagtca agttcttttc ctagtttacg atggtctctt
     2521 tcttttgctt cttcacggag acggatatat tctttcaaag ctttcttgtc aaaaaaggct
     2581 gtcccgtata cacgttgcat catttgattg tttgagtttc cacgccaata agcacctgcc
     2641 actgataata atttaaatac ttgtatacgg ccagtggatg gaacatgtgg tccacgacac
     2701 aaatcgatga attcgccttg ttgataagca gtaatcactt cttcttctgg caattcacta
     2761 atcagttcta ctttgtacgg atcacttgca aaaatttcta atgcttcctg ttttgaaaga
     2821 acacggcgtt cgatcggcag attttctttg acgattttca tcatttctgc ttcgattgcc
     2881 ggcaaatctt ctgcagttac ttggttttgt ccattatctg tatcgtagta aaaccctgaa
     2941 tcgatagctg gtccaacgcc aaagtggata tttggataga ggcgacgcat ggcttgggcc
     3001 atcaaatgag ctgctgaatg acggactaag ccaagtgcat cttcatggtc tggtgtgatg
     3061 atctcaatac ttgcatcttc ctcgattgga cgagtcaaat caacgagttt tccgttcact
     3121 ttaccagcta aagctttttt tgctaagctt tttgaaatgc tttcagcaat tgccaatgtt
     3181 gttgttccag actcaaattc tttgactgca ccatctggaa aagtaatttt tatcataata
     3241 gtttcctcct tgaaatgttt tgattgatca gatcatcaag ctgaaaaaca aaaaaatcct
     3301 agcatctctt gaaaagagat actaggacga aaagttcgtg gttccaccta atgttcaaac
     3361 gcaaatgcgt tcctcaagcg ttgtaacggt ccgaccgccc ttgcttcaac aagggtttcg
     3421 aaagtggtca tcctttggtt ttctcctgga aaagtttcag cctagctttt cctctcttat
     3481 cgaagactct caaattcagt tggctttctc atcaatcaat tatgtgttta tttaaccact
     3541 aataaataga aaaatcaagt gggaaactaa acttattttt tccattcccc atttacaccg
     3601 tatgttcctt ttttcatctc attgatgatg acatggatgt gttcttttgg tgcctgcgtg
     3661 tttttatgaa cagcttccgt gatatctttc atcatatttt ctaattgttc cggactgcga
     3721 ccttctacta attcgacatg tacgaatggc ataatgctct ctcctctttc tgatcgttct
     3781 tttttcaact cgattctatc atactatgtt tgtcttgggt actgaatcag gaaaaatggc
     3841 tggatctttt tttgctctca aggcactcaa aatagagacc gtatccacga cttcttgcaa
     3901 aacagctccg aataatgcgg ggattagtcc cgtacttgca attaacatca aaaaaataca
     3961 aataaagatg ccaatcagta cagactgttt cgctattctc atcgtatctc ttgaaatcac
     4021 tactgctttt gctacacgca agagatcatc tttcaagatg accgcatctg cactttcact
     4081 agcagctgta gaaccatgag cccccattgc aataccgata tctgcagcag caagtgctgg
     4141 tgcgtcattc acgccgtctc ctaccatcgc aacaggtctt tgatctttag ggatactttc
     4201 taaaatggtc agtttatctt gaggcaagct acgggcatgt acagaatcta attggacttt
     4261 ttctgcaatc gtttgagcaa tggcagatcc atccccggtg atcatcatca aatttttcac
     4321 atcaagtttt cttagctcct tgattgtctt tgctgcttct gggcgaatct gatccgaaaa
     4381 taggatcgtt cctgcaaaag atcgatcaat tgttacatag attgctgtat tggacgtttc
     4441 tatttcttca gctgaaacaa aagaggcacg tccaatcttt acttcatgat tattgactac
     4501 tgcttggatt ccttgaccgg tcacttcttt cagttgacta acaggataca gttggctgtt
     4561 tttcacgttg ttcactaacg aacgagctaa gatatggcta gattcctgtt ctgcactagc
     4621 tgcatacaga agtagctctt ctttttttat tccattgact ggctgaatcg cctctacttc
     4681 caatatccct ttggtaagcg ttcctgtttt atcaaaagca atcgtttttg tacgtgcgag
     4741 cttttcaatc gttgttccag tttttacaac gatcccgttt ttacttgaac ggctcattcc
     4801 tgcaactaaa gcaatgggcg cagctagaat cagtggacaa ggtgatgcca ctaccagtac
     4861 ctcagcaaat cgtaaagggt cttttgagaa ccaccaagaa ataccaccga tcaaataagc
     4921 aatcaacgtg aaagggactg cataacgatc agctaaacgg acaaatcggg caggcttttc
     4981 ttttgattct tcgactagtt taataatcat ttgatactgg ctgtctgcgg cacgtttttc
     5041 agcgatcatt tggatcgatc cgtctccatt taaagaccct gacataatct gatctccagg
     5101 tttcttggtt tccgggcggg actcacctgt caaagaagat tcatcgaccg aagattctcc
     5161 tttggtcact cgaccatctg cgggtacaag ttcgccaggc ttgactagta actggtcatc
     5221 aatatttatt tcatcgatta ctatatcctg caattttcct tctttgaatc gatgggcaat
     5281 tcttggggtg ttatctaaca aagactgcag ttctttaccc gctcgtttat tcgcgtaatc
     5341 ctctagacta tctccgcccg taagcatgat caatatcatc aaactagccc agtattcttt
     5401 gacggctaat gttgcaagga ttgcggtaat cgctaataca tctacgccat atcttcctga
     5461 acgtaacgtc ttgatcattt ccaaaaacat caagaaggcg agaatccctc cagtaaatgc
     5521 gacaatcgca aatgcaaggg ccggctggtg aaaaacaaat tcacttaata aagctatccc
     5581 accaataagc aaagtaagta aaaatttttt gaaatgtgtc attgtttttt ctcctctttt
     5641 atgtgactta gagaggttag taataaattt ccagctgcct ctttcatctt aagcatagtc
     5701 cttgactagc actttttaaa acaataggcc ttaattgaaa acaactatca ctatcagcgt
     5761 ttccaaaatt catcagttct acaaacaaaa atattcagca aggattactt tttcgattca
     5821 tcagttaaaa agtacctttt ttcgaaaaac tatgctatac ttttaattcg ttatttacgg
     5881 cggaaggagc aaacatgcga tgaatgagaa acaactagaa caaacgcacg tggatgaaac
     5941 gatcgaacta atccagatcg aacaaaatat gctggataca aaacaacaag aattgactca
     6001 ggaaatgaat cattcgacaa aagacacagc taatcacaag attcgagctg gttccaatga
     6061 agctttttat gaatctgctg ttgaatatcg ccaacatgag caggaattgc ttttgaaata
     6121 ccatactctt gaagcacagc aaaaacgcct gcaaacgttg tctgtcatga aagatagtcc
     6181 ttattttgcc cgtatcgact ttaaagaaga ccgtgaaaca gaaacattat atctaggtat
     6241 cgcttcttta agagatctgg cagaagaaac aatcgttatt gactggcgcg caccaattgc
     6301 caatctttat tatgaaggtg agattggtcc cgcattttat gaaactgaaa cagatagaat
     6361 agatgtcgat ctgcagttga aacgacaatt taaaatccaa gaaggacgta ttttatctat
     6421 ggtagatacg tcggaagtaa tcaatgatga ctttttgcta gagatattgg atgaatcatc
     6481 aagcaaccat atgaaaaaca tcgtctctac gatacaaaaa gcacaaaatg caatcattcg
     6541 ggacacaaca agtaaagtaa tgctgattga aggaatcgcc ggaagtggaa agacatccgc
     6601 tttgcttcaa cgtattgctt ttctgttgta tcataatcgc aagtggctcg aggctgaaaa
     6661 cgttcttcta ttttcaccca accatttgtt ttctgattac atctcgactg tattgccttc
     6721 attaggtgaa agcggcgtgc ctacacagac attcaaaaat tatttggtgc agctgctgcc
     6781 agagttttct ttactagatg aacagcaaca agaaagtggt tttttgctag gggaaaaaga
     6841 tccgatacaa gtgatgaaaa gcggattgac cttagtcgat cagattgatc gctatatcca
     6901 aggcatcaca aactacggtc ccttgttccg tgatatgaaa ataaatggcc gtacgatcct
     6961 atcaaaggaa tcgattcgcc aatggtataa agaaacaaat gaacaattac ctttacatca
     7021 tagactttct ctattacaaa cgaagctcct gaaaaaatta ggcggtctgc aaaaagacga
     7081 aacacgtcag caatgggtca aagatctggc ggaagaacag ctccaagaac tatacgcaac
     7141 agatccaaat cttgaatata cagaacaaaa ggagcgttca ttacgtaaaa aactggccaa
     7201 ccaaatcgta cgtaaaagat tccgtcgaat ccatcgaggg atcatgaatt atcaatttgt
     7261 caatataacg aaacaatatc tccactttct acaaactgtg ccactttctc ttctaacacg
     7321 acataaaatc agcgagacca ggtggcagga acatttgaca actgttcgta cacacttgcg
     7381 tcaaagagaa ttgcgtcaag aggatgctgt actattcttt ttactgatgc gccgaacata
     7441 tccagtgacg gttacacaca aagctcgcta tatctttatt gatgaaatgc aggatttcgc
     7501 accatcacaa gtggcattgc tgcaagcctt gttcccaaaa gcaaatttga ctttctgtgg
     7561 agatctaaat caaaatgtct ttggaaatga aacgatcgtt ggatcattgg aagacctttt
     7621 cgtggaccgt gaagtcactc gtttccaatt aacgacaagc tatcgctcca caaaagaaat
     7681 cactgatttt gctaatcatt ttattgtgaa agaacatcaa gtagaaacaa cagcaagaaa
     7741 aggagacctt cctttgctca tccaaggaac aacagatgat atcaaattga attggttagc
     7801 agataccatt cgaacgactc agggaaaagc ccggtattgg cgtacagcaa tcatagctaa
     7861 aacacaagaa gaatgcgaag ctctttatgc tcaactgcca gacgagttga aacaacaagt
     7921 ccagctgatt ttaaatgaga gtgactttat gaagcgttcc attattttac ttccagcctt
     7981 tttagcaaaa ggtctggagt ttgaccgagt tttcttgtgg gatgtagatg cagaacattt
     8041 ttcttctcag caagacaaat tgatccttta caccatgtgt acacgtgcga tgcatgaatt
     8101 gacccttttt acctccaata atgaaagtcc tttgttaaac aatatggata tttcatctta
     8161 tgagaaaaaa gtactgtaag taaaaagaca actgctatca ttaatgttag cagttgtctt
     8221 tttatttaca ataataattg cagaaaaagt aattcaatca tttaaggtca tttattctat
     8281 atataaatcc aaatctgttt caggatattt taatttgaca agcgaaataa tcattttcag
     8341 ttcttccatg ttttctggat attctgggtc aaataccttg ctatgacggc caatcccgcc
     8401 attgtcgatc aatcggtaaa ttcgattcca ttcaatgaat tggtcaattg attggaaatt
     8461 gctgattgga tagccgccac aatccaaaat ctctgataca cgaaatgcta ggtatgcact
     8521 atcgatcact ttattttttt tcgtgtctat cttctcattt ctttcgataa gaattttttc
     8581 aggacgatgt ttcaagctcc actctaatag tagaaagaaa gcctccatcc gatactcttc
     8641 ttcaaataac cgaaaggctg aatcagaacg acgaaaaagc agcggaatca gataattttg
     8701 tctataaatc agcattggtg cgcggtccac catacactct aaataacggt tcagataccc
     8761 tgtaccgaca tagtttccta atcgatagcc ttcaacgccg aatcttgcgt atttttgcgc
     8821 taattttgaa caatcagtta cgttcacagc taactcttct actttgcggt aaccgcttaa
     8881 gatacgttca aaaggttcta aagtgatttt tgcttccata ttcttccccg tccatcttcc
     8941 acatacttca tatcatttca catgatactt atgctctcat cataccacag gttgaattag
     9001 aaaagaaaat aaatcacaca aaaaagcaag acaatctgtt agtgagattg tcttgctttt
     9061 cgaaaaagac tgaatacttt tcgttaaaac tggttgctta gtcttctaat gccacattat
     9121 ggaacacatg ctgtacatct tctagatctt ccagtgcatc aatcattttt tcaaatgttt
     9181 cttgatcttc tggagatagt gttacctcgt tttgagggat catttggatc tcagcgattg
     9241 aaaaatcagt gattcctttt tcttttaatg cttcttgtat actatggaaa tccgctactt
     9301 caccgtaaac gatgatttgt ccttcttctt ccattacatc gcgaacatcg atgtcttttt
     9361 ccattaggta ttcaaggatc tcgtcagcgt cttctcctgc aaaagcaaag attgctgtat
     9421 tgtcaaacat gtaagaaacc gctccggaaa cacccatgtt cccgccattt ttcccaaatg
     9481 ctgcccgtac gtctgctgct gttcgattca cattgttcgt caatgtatca acaatcacca
     9541 ttgagccgct tggtccaaat ccttcatagc gcaattcaga gtatgtttca tctcctgcac
     9601 cttttgcttt ttcgattgct cggtcaataa tatgttttgg tacattataa gttttagcgc
     9661 gatcaatgac gaaacgtagt ttttggtttg catgcggatc aggatcacct gattttgctg
     9721 ccgcgtagat ttcaatccca aattttgcat aaatccggct attattagca tctttagcag
     9781 ttttctttgc tacgatattt gcccatttac gtcccatatt ttcacttcgc tttctgattt
     9841 gatagtttca taacgcaaaa acgtcctgtt ccattatact tgtgaatcac gattctgtga
     9901 agcttttttt ctttcttcct tcattcttct catttttagt caattatcta tactcggatt
     9961 gggatttgcc cgaaattctg ttccggaata ttgcctccca attgatagat ttgatcactt
    10021 ctaactaaca gttccgcttc ttgtgcctct ttttttccga ttcgggaata aatagaaatc
    10081 tctttttttt cttttaagaa tcttcttcca gcatcggtaa atccaagtag tcgaagataa
    10141 tttttatttt gttggacagg aatctcttgc tttttgatat tcagcaagac ataacaagat
    10201 aatcgttgga tccgtgtcca tgtatatctt tttgttttta cctttgacac aaaatcctca
    10261 aaactatcag cagttgcagc tgctgttttc atgcgcgatt ctattccttc ggtcatctga
    10321 tagatatctg tcagttccag caaagtagaa gataacaacc gataacgcaa atacggccaa
    10381 taagattccc aagtctgcag cgaacaagtt gaaagatctt cttctgtctg aggcggcaca
    10441 agtccagcta ttctttttcc ttcttctaac gcatggcgaa tggctgttgc actggctatc
    10501 ctctgatctg tgatgatttt ttcatgatag cctgcacttt tacgtcggat cggatgtaat
    10561 tccatagggt ggacatactt ggcattttcc tttgcataac ttaatcccaa gatatgattt
    10621 ggcgaagaaa aatcaagcgt aatatcggga tacagactgc gaaaaacctc agtcattttt
    10681 tgcggataac tatacgtttg atcagacagt tcttgaaatg cctcttctat cctatcttga
    10741 ttctcttgga caaagcgtcc gaaactgctg taatcaaacg aagtctcttc ttctgttcca
    10801 aaacacaaac tgtcacagtc cagtgtttgc aagattttta ctgcaccttt agcaaaatag
    10861 tcagctgact gaaccgccca ttcgacggga agctccacta ctaaatccac accattttgt
    10921 aaagcttcat tcgcgcgatg ccatttatcg atgatagccg gttcgcctct ttgcaaaaaa
    10981 ttcccgctca ttacagcaat gacgacctct gctcctgtta tttttcgtgc catttccaca
    11041 tgatagcgat gtccattgtg aaaaggattg tactctacga taatcccgca gcttttcatg
    11101 cgcatcctcc ttcatagtta tcgataaaaa agaaatagag acaagtaaaa aaaggcaaaa
    11161 atcggaaaaa atacccgaac tatataaact attctcgcaa acacaatact tctgcgacga
    11221 aaagctgcac tgcaagagca atgcctatat cgttcggata tataatttat gttgaagatt
    11281 tattcttttc ttgttaactc gagcaggcaa aaccgctagt ctttgtatgc tacaaagaac
    11341 cagcgctttg atttctctga tgctgcttcg tctgtaaaat cagaaaatgc ttcgactttc
    11401 gaaaagccgc tgttttccag cattcttaaa taactttcca atggataggt acgctcttga
    11461 tgtagttcat cttcacgaat aaatcgacca gacccttctt gttcttcaac gaaaaaggta
    11521 agaaaatgtt cgatgctgtg tttctcgctt cctggataac tatcccacaa aaaagcaaaa
    11581 ttatcccctt gataatgata gctatattct ggaaaaacct catctacttg ataggtggag
    11641 tgtacgtcaa ataaaaatcg gccattttct tctaacgctt gatagacttc atcaaacact
    11701 tgctgtacct cttgtctatt tttcatatag cacagcgaat cagaaaaaca ggtaattgcc
    11761 tgatattgtc cgatttctga taaatcgagc atattgcctt ccacaaattg gatcgatgcg
    11821 tcttcttcat atgctcgctg gctggcaatc atcaacatct cttctgaaag gtctaaccct
    11881 gtcactttat atccatcttt tgcaaaggca accgctaatg ctccagtacc gcatgccagt
    11941 tccaaaatat tttttgtgtc ttcttgtaaa tgacgtttcg aaaaagctaa ccattgctga
    12001 taaagtgttt cgtccatcac ttcatcgtaa atgaaagcaa atgtttcata agccatatgt
    12061 taatccaacc attcactaag atcgactagc ggagcatctg accatagttt ttccagatta
    12121 tagaaatttc gttctggcgc atggaaaaca tgtacgatca catctccaag atcgatcaat
    12181 acccattttc cgccttcttt tccttcaata cgttttactt catatttgtt ttcttcttct
    12241 ttatcaatga tttcttctgt aattgcgttg atttgacgtt cgctatttgc tgaacagatc
    12301 atgaaataat ctgccaacaa ggaaacattt cgaacgtcta aagcgataat gtcttctgcc
    12361 cgttttgaat ctgctgcttc aatcgcaatt tttaattgtt ctttactatc tatgatgatt
    12421 tcctcctaat atgagcagct tatttttttg ctacccagta attatatgtt tcgattgttt
    12481 ttggataaat ctgttgttct tgcgcaagca aatgggccaa tgtatgtttt gtttcaaatg
    12541 ctacagcttc gtctagatca gcccatgcaa tggctcgtgc atcttgtacc ccaggaaaat
    12601 ctcgacctgg ttcaatatag tctgctacat aaatgatttt gtcaaggaga ctcatcttag
    12661 cagccccagt cgtatgcacc cggattgcat gaagaatctc tgcatcggtg attcctaatt
    12721 cccgctctac aaacgaagcg ccaacaagac catgccagat tgcattatta taattcaata
    12781 actccggatc aaaaccgtct cttacgatta ccattttgaa ctcctcatct ggtcgttctt
    12841 ttgcataatc atgtgtcaat gcagcaatac tagctttttc aggagaagca ccgtattttt
    12901 tggcaagagc cacggctgtt tcttctactc caagcacatg cttgaagcgt tgttcgctca
    12961 ttctcatctg tattttttgc atcaggtttt ctctagaata agcagtatat tcattactat
    13021 agtccatatt catacaaccc ttcattttga atataatcaa ttactttatc tggaacaagg
    13081 tagcggatag aacagccttc tttgatcttt tgacgtattt tcgtggaact gatgtcgatc
    13141 tcaggtacat ctacccaaat tacaggatat ggagtatccg ttgtgtatcc tggtcgacgt
    13201 atacccacaa agttgaccat agaagtcagc tcatcgattt tataccattt tggcaaatat
    13261 tctaccatat ccccaccgat aataaagtaa taatctgtat ccggattgtt ttgcgtcaat
    13321 tccttcatag tatcataggt gtaacttttc ccaccacgag ctaattcaat cgtttcaatc
    13381 tgcaaaaaag gattgtcttc caccgctaat tccaacatgt tgagtcgatg ttttgcatca
    13441 atcgtctgtt tctcatcaac atgaggtggc tgataagaag gcatcaaaaa tacgcggtca
    13501 agacctaaat tccggccagc ttgttcagcc atcactaaat gtgcaaggtg aacgggattg
    13561 aatgttccgc ccaagatccc tacttgcttt ttcttctcta agaattgagg catttcttca
    13621 ggaaataatt gtgaacgaac aaatgtctta gcttgcgtat tcatcatggt cactccttat
    13681 attgctttta cttcttttga tagcctttga tacttttctt ttgtggaagg tttgtataat
    13741 acaataacac gtccaatgat ttggacgatt tcgcaatcta tttctttttc taatacttga
    13801 gctgcttctt ctgcaatctc atctgtattt tgaagtaacg atactttgat cagttctctt
    13861 ttttctaaag cttcttcaat ttgaacgatc attgctgaat tgatcccacc ttttccgatt
    13921 tgaaaaatcg gctgaagatg atgcgctttg ctacgtaaaa aacgcttttg ttttcctctt
    13981 aaactcataa ttttccttct tccttaaatc aatgctttgc gtttcaatac atctacaccc
    14041 tttggcgccc agcccgcaat tactactggc gctgttacag tgatccagcc tagaccggca
    14101 aaaacgatgt ctgttttttc tttcacagaa aactcaaaac gaacaagctc cggaaatgcc
    14161 tcgacttcat caggacgagg aggctgtaat aatccaccta catgtttttg ataaaattcg
    14221 tctgcttttt ccagtttcgt tcgatgaata tttacatcat ttgacacata agcaataaat
    14281 gaagcacgat tgccgctgat aaaatcaaat cttgcaagtc cgcccaagaa aagtgtttgc
    14341 ccttcattca gctgataaac tttaggtttg atttctttta acggtgcaat gatccgcaaa
    14401 tcttttttac ctaagtagtg cgccatttga tggcgatgga taattcccgg tgtatcaatc
    14461 aaaaaatgcc cgtcatctaa aggaatctcg attttatcca aggtcgttcc tggaaactgc
    14521 gaagttgtga ttacatcttg tactcccgct gtatttttga tgatttgatt gataagcgtc
    14581 gattttccga catttgttac tccgacgata tagacatccc gtccatcgcg gtattcttcg
    14641 attttttcta atagttccgt catctcatga gatttcttag cactagttaa taatacatct
    14701 actggacgaa ttcctgcttc gtaagcacgt tccttcatcc attgtgttaa tttagttttt
    14761 ttcaatgatt taggtaatat atccactttg ttcccaacta aaagtactgg attgtcaccg
    14821 acaaaacggt gtagtcctgg gattagtgaa ccattgaagt caaaaatatc caccacattg
    14881 acgatcaatg catctttttg ccctaattca ttcagtaagc gcaaaaagtc gtcatccgtc
    14941 aatgagacat cttggatctc attataatgg cgtaatctga aacaacgctg acaatagact
    15001 tccccggaag ctaaaccttt ttccaaagcc gtttgcggcg tgtatcctaa ttcaccgggt
    15061 ttgtttgttt gtataacggc accacagccg atacattgta attcttctgt cattcgattc
    15121 cacctcgcca gcccatatca gggtgttttg ctaatagata ggccataatt cttttttccc
    15181 aaaaacgatt aaattgtgtc ttccaactat ctgttgcgat aatgggtttt acaaggatgc
    15241 tacgtatccc tgcacgattg gctccacgta tatcagtcat aatctgatcg cctaccatta
    15301 ctatctcatc atctgctaaa tccaattgtt tctttgctat gttgatccct cttgctaaag
    15361 gtttcattgc tcgtgcaaca tacaaaagat caaatttttc aatcgctcgc gcgacacgtt
    15421 ttgaattatt gtttgaaaca acaacaacag ggattcctgc atttttcatt tctaaaatcc
    15481 aagtaagcaa ctcttctgtt ccatctggat tattccatgc aatcaacgta ttgtctaaat
    15541 cggtcaatac tgctttgatg cctagattct ttaattgggc aggtgtaatc tgatagatgg
    15601 cttctaccat ccatgttggt ttgtaatttg aaaacatgct gtctcctcat ctgcaaaaaa
    15661 tgaaggagca cataaagtac tcctcaaccc cgtattatac tgcatatcag caaaaaaaac
    15721 tacctctata ctcgttcgtc tttcagaaaa agcatttctt tacaaaatga ccaaaaataa
    15781 agattgatca cagaaaaatt tagaaacaaa aaacaactaa tattggcgga acctcttgta
    15841 tcgtttttcc agtaatttat gctgatctaa tcgaacaagt tccgtcaatc tttcaatcaa
    15901 cgcttttttc ataatgtgtg cgactttata tcgtggcaac tgctcgccgt tgaacgtttc
    15961 aggaataact cgatcaacga ttcctaactc gcgtagttct ggcgcagtaa ttttcatcaa
    16021 ctcagctgct tctgcggcgc gactaccgtc tttccacaaa atcgacgcaa atccttctgg
    16081 agaaagcaca gcataaatcg tttcttccag catccagact tcatccgcta aacctagagc
    16141 aagggcaccg ccacttccac cttcaccaat gatgatggcg atgatcggta cctttaaatc
    16201 agacatttcg agcagatttc tggcaatagc ctctccttcg ccgcgctctt ctgctcctac
    16261 accgcaatat gcacctgctg tattgatcaa ggtaatcacc ggtctattga acttttccgc
    16321 ttgtttcatc agtcgtaaag cttttcgata gccttcagga tggggagaac caaaatttct
    16381 gcggatattt tcctgcagat cccgtccttt ttgaataccg atcactgtta ccggtttgcc
    16441 atctaatcga gcgattccac caacaatcgc ttcgtcatct gcaaataaac gatcaccatg
    16501 gcattcctcg aaagaatcga agatctcttc aaaaaaatcc aagcttgtcc atcgttcttt
    16561 agctcttgct aactgaacga catcatgtgc tgtcttatcc acgatctatc catcctttct
    16621 gtgtatgcaa ttcaagaagc gtatggattt tctgtttcaa ttccatgcgg ggaacaatct
    16681 gatcgacaaa accatgagat aataagaatt cagccttttg gaaatcttct ggaagttcct
    16741 gcttgatcgt ctgttcgatg acgcgtcttc cagcaaaacc gatcaagctt tggggttctg
    16801 ctagaataat atcgccttcc attgcaaaac tggcagttac tcctccggtc gtcgggtccg
    16861 ttaaaacagt tagataaaaa agaccttcat tgctatgccg tttgacagca gcagagattt
    16921 ttgccatctg cattagagaa aagattcctt cttgcatccg tgcgccacct gaagctgtga
    16981 acaagacgac tggaagactt tcctttgttg ctcgttcgaa taatctggtg atcttttctc
    17041 cgacaatcgt tcccatactt cccatgataa aattaggatc catcactcca ataatgaagg
    17101 gaatcccctg aatggttgct ttaccagtga ggactgcttc atgaagtcct gttttatctt
    17161 gcattttttt aattttttcc gtatattcgg gaaatgctag cggatctttg gttacaagat
    17221 ccgtatccca ttcttcaaaa cttttttcat caataatcaa agctagacgc tgccatgcac
    17281 cgattcgaaa agcgtatccg caatgagggc atactttttc ttcaccgatg tctttcgtat
    17341 aaaggatatg tttgcaattt ggacactttg cccacatgtt atcaggaaca gatggcgagt
    17401 tagcaggttt gctctctcta ttaggattga tccgaatata tttctttttc ttaaacagag
    17461 ccatctatgc gcctcctatc tccgtttctg gtgtccaatt cggtaaaaat tcttcttgta
    17521 aaaatgctgt gctgtaatca cctgctatga ctgcaggatg actaatcaaa tccatctgaa
    17581 attctgcgtt cgtgatgatt ccttctgtga taaattcact taaggctcgc tgcatcttca
    17641 ttaatgcttc aaaacgcgta tttccatgga cgattatttt tgcaatcatt gaatcataat
    17701 atggcgggat cgtatatccc gggtaaactg cactgtctac tcgcagtccc atcccacctg
    17761 ctggaagcag taagttttgg atcttccctg gagaaggagc aaaatggaaa gcaggatttt
    17821 ctgcattaat ccggcattcg attgcgtgtc cttggaatac tacttcttct tgacacacat
    17881 tcaaaggctc accggctgct atctccacct gtttttttac taaatcgatt cctgtgacca
    17941 tttctgttac tggatgttcc acttggattc gagtattcat ttccatgaaa taaaaatcgc
    18001 cttcttcatc catcagaaac tcgatcgttc cggcattttc ataattgact gctttagctg
    18061 ctcgaacagc agcgtctcct agtgcttctc tttttgtttg tgagatcact acagaaggag
    18121 attcttccag tactttttga ttgttgcgtt gtaaagaaca atctcgctcg cctaaatgaa
    18181 tgacatgacc aaatcgatca cctaagatct gaacttctat atggcgagca ggataaatga
    18241 ttttttccaa atacatatca tcatttccaa atgccgctgc tgcttcttgc tgagctgaag
    18301 aaaaatgctg tgggagctct tcttttgatt gaactttccg aatgcctttc ccgccaccac
    18361 ctgcagctgc tttcagcatc actggataac caattctgtc tgctatttcc aatgcttctt
    18421 ctactgtaga taatacacca gtacttcctg gaatgactgg gacatttgct tcttgcatca
    18481 attgacgcgc attgatctta tttcccattg catcgatcgt tgcactttta ggaccaataa
    18541 atgtgatatt gcactcttca cacatggagg caaatcggct attttcagat aaaaaaccaa
    18601 atcctggatg aattgcttcg gcttttgtca cgattgcagc gctcagtact tcctgaacat
    18661 tcagatatga atcaacggct tttgccgggc caatgcagat tgcttcatct gctaactgtg
    18721 tatgaagtgc ttcggcatct gcttctgaat atatcgctac ggtttggatc cctagttcgc
    18781 ggcatgcacg aatgatacgt acagcgattt caccgcggtt agctatcaat actttagaaa
    18841 acatatttca cctatccaat cataaaggtt aattccgctt cggctacttt tttaccgtct
    18901 actttagcga ttcctttgcc tattccggct gaagctttca ctttcaatat ctcaacttca
    18961 aggtataagg tatctcccgg tgtaactttc ttacggaatt ttgctttgtc caatccgccg
    19021 aaataagccg tctttccctt gaattgttca agagaaagta atgcaactgc tcctgcttga
    19081 gccattgctt caacaatcaa tacccctggc ataacaggtt cttctggaaa atgtccttga
    19141 aaaaagggct cattgatcgt tacatttttc ttagcaacga tccgttctcc ttcaaccatt
    19201 tcttccactc gatcaatcaa aagcattgga taacgatgtg gaatgatttc ttttatttct
    19261 tgtatgttca ttgttttccc tctttcctgc tttgcttatt tttgctgaat cctaaagagt
    19321 ggctgtccat attcgacaac tgactcgttt tctacaagga tttctgtgat cactccgtcg
    19381 gcctcactag ttatctcatt catcagtttc atcgcttcaa taatgcagat aacttctcct
    19441 ttggcaactt gatcacctac ttttttgaaa gctggtttgt cgggcgctgg ttgcagataa
    19501 acgattccca cgattggcga aacaacctcg attccatcaa atactgccga ttctttttct
    19561 ttcattattt ctggagcagt tgttgcttgt tcagatatga cattgactgg ctccgaaact
    19621 ggcaacgttt cgtttgtttg aaccatattc atgcctcgac ttgttttatt tttattcata
    19681 tacaattcaa attgaccttc acgcaaatcg aattccgtta gcgaggattg atcaaattga
    19741 ctgactaatt ctctgatttc attgatatcc aaaattattc ctcccagcgt ttcatgcaca
    19801 gaaccgcatt atgtccgcca aaccctaatg aattactcat tgcataagta aaacttgcag
    19861 gtttgctttc gtttttgaca actgtcaagt caatctcagg atcttgtttt tctacattga
    19921 tcgtaggtgg gataaattga tgttctaatg ccagcaaagt tgccaaggct tcgatcccgc
    19981 cggctgctcc caataaatgt ccagtcatcg atttcgtgct gctgacgtaa gtatctctga
    20041 aattttcacc taatgcgatt tggattgctt ttgcttctgc ctggtcattg gctggtgtac
    20101 ttgttccgtg agcattgata tatccgactt gttccggctg tacaccagct tcttccatcg
    20161 caagcttcat tgctttagat gcaccagaac catctggagt aggagctgtc atatggtaag
    20221 cgtcacagtt tgcaccgtat ccaacgattt ctccatagat tttggcaccg cgagcttgtg
    20281 catgttctaa agattcaaga actagaacgc ctgctccttc acccatcaca aaaccattcc
    20341 gattttcgtc aaacggaatc gaaccacgat taggatcagt tgattctgag agtgctgtta
    20401 aagctgcaaa tccagcaatc cccatctcac aaatcgttgc ttctgttcct ccagcaagaa
    20461 tgacatcaga atagccatgt ttgatgttac ggaatgcttc cccaattgaa ttcgttccgc
    20521 tagcacaagc tgttacgata gctgtacaaa taccttttgc acctacacgc aaagcaatat
    20581 ttccagctgc catgttggca atcgccattg gtacgaacat tggagaaact cgttttgcac
    20641 ctttttcatg tagacgaata acttggtttt cgattgttgg aagaccaccg atcccagatc
    20701 cgatgatgac gccaaaccgg tcttgattca ttttttcttt atctaaaccg ctcatctcca
    20761 ttgcttctaa agcagcgtag attccataga tcgaaaacat atccattcgt ttgctatctt
    20821 ttttaataaa atatttatcg taagggaaat ctttgacttc acccgctaag gtaatccctg
    20881 tttctgaagc atcaaattta gtgatcggtg cgatgccatt cgtcccctct tttaagctat
    20941 tcaaaaagct gtctgcgtca tttccaatag gtgaagtgac tccatagcca gtaattacca
    21001 ctcgattcat ttgtttgctc tccttttctt atccgtgcat gaccaatccg ccatcgacat
    21061 tgatgacttg tccggtgata tatggatttt gtgctaagaa aactgctgtt tgtgcaatat
    21121 cttctacttg tccgaaacgc tgcatcggaa tttgcttttc cgcttgttcc ttgactttgt
    21181 ctgctaaaac ttccgtcata tcagttgtga tgaatcctgg cgcaatggca ttacatgtaa
    21241 tgcctctagt tgcagcttct cgtgccacag attttgttaa tcctactacg ccagctttac
    21301 tagctgcata atttgcttga ccaatatttc caatcaaccc agatacacta gagagattaa
    21361 tgatggcccc ttctcgctgc ttcatcattt ttttcaatac gtgctgcgtc atattgaagg
    21421 ttccaatcaa attgatatcc agacattttt tgaaatcttc tgcatccatt cgcatcacaa
    21481 gtttgtcatt tgtgattccg gcattgttta ctaacacatg gatcgatcca agcttttctt
    21541 ctgcttcttt gatcatttgt cccgctttct cataatcaga gatgtcaccg gaaactccta
    21601 cgcattttac accgaatgct tcgatttctt ctatcttttc ttttggaatc tctccgcgac
    21661 cattcaaaat gatatttgct ccagcttttg caaaagctaa ggccatcgct ttgccgattc
    21721 ctcttgtact acctgtgata aatacatttt ttccttttac atccataacg ttcctcctta
    21781 tagtattgct attgtttcat caaatgtctg cttattctca acacgcagaa ctggtattgt
    21841 tttatcgatt tttttcataa atccgcttaa tacttttccg gggccgactt cgatgacttg
    21901 ttcgatgccg attgttttta acttatggat actctcatag aaacgaacag gttgcatgac
    21961 ttgctgttca agtagtcccg cgattgtttc tttcttcatt atttccgttg ttgtgttgct
    22021 tatgatagga aaagaaggtt cagagaattg aatctgactt aggtccttcg ccagtttttt
    22081 tgctgccggt tctaaaatag ctgtatgaaa aggcccgctg acatttagag ggatcattct
    22141 tttgaaccct ttttctttta atagtaaaac agcttcgtct actgcttttt cttcgccacc
    22201 aatcacgatc tgctgaggtg tattataatt tgcaggagat acgatgccgt acttgctagc
    22261 ttcatgacag ctttcctcga tcgtttcaat cggcgcattc atcactgcaa ccattttccc
    22321 gcttccagct ggggtagctt ctgtcatata agcgcctctt tttgctacta acgcaactgc
    22381 ttcagtgaag gatagcgcat tactagctac taatgccgaa tattctccta aactcagtcc
    22441 taaagctgca tctgggatga ttccatgctc cttcaataag cgataatacg cgatgctgac
    22501 tgttagaata gctggctgtg tatattgcgt ctgatccaaa cgttcatttt ctgtaaagca
    22561 aagttcagcc atatcgtagc cgagaatctc acttgcttca tcaaatgtct gctttacaac
    22621 ggcttcttca tataaatctt ttcccatacc ttgatattgt gcaccttggc cactaaataa
    22681 gaatgctgtt ttcatgtttt tctccaatat atccagaata tatgtttgat tcttcctatt
    22741 caaaaaggcc gagacattcc tatttcagga aatgtcccag cccccaacct aattttaatg
    22801 attatgcagt ttgtttttct acgtaagtca ctaaatcttg aaccgttttg attccgtctt
    22861 ctgtttcgat tttcacgtca aattcatctt caatttcatt gatgatttgg aataaatcca
    22921 agctgtcagc ttctaagtct tcttggatgt cagtagtcaa tttgatttct tcagtttctt
    22981 ttcctaattc ttctgcgatg atttcttgaa ttttttcgaa taccataata atatattcct
    23041 ccgatattgt ttgcttttta tttttataat ttaacagtct acaactccat gagaatactt
    23101 ccccatgtta aacctccgcc atagcctgta aatattactt tctgctgttt tcctaaggtg
    23161 atctttccac tagaaactgc ttcatccaat aagataggga tgcttgcagc agatgtattc
    23221 ccatatttat ccatattggt caggaacttt tcttgcggca tctttacttt tcgggcaatt
    23281 ttctcaataa ttctgaggtt tgcctgatgc aaaagcagat aatcaactgg tttgtctttc
    23341 atgacttctt ggatattgtt tgcaacgtct cgtacggcaa aatcaaaaat atctctgcca
    23401 gtcatttgca aataatacga accttctgaa tcttctgaat agaacggatt tttattttgc
    23461 tggtagccag cggtcaatga gctacttctg gtaccgtcag ctgctaattt ttctttcagc
    23521 aattttttct gtggattcgc ttctaagaga atcccacctg ctccatcgcc aaacaacaca
    23581 gcagtcgtac gatcagacca atcgattagc ttagatagtg tttcaccacc aatcaccagt
    23641 cccttagttg agccgcagcg gatcattttt tcagccatgc ttaacgcata aacaaatcct
    23701 gagcacgcag cactgatatc aaaagcgata gcatgagttg ctcccaattt cccttgtact
    23761 tgtgctgcta cagatggtgt cgtgtaatcg ggcgacattg ttgctactaa aataaaatcc
    23821 aaatctttag ggtttagatt tttctgtttt atcaattttt ccgccactaa aatgcataaa
    23881 tcagaagtct cctgatttgt tacacatctt cgttcacgaa tacccgtcct tgacgaaatc
    23941 cattcatcag aggtatccat catcttggaa agatcattat tagtaataat cttttcagga
    24001 aggacgctgg ctgttgcagt aatcattcca aattgttcca ttaaatatcc tcacttatag
    24061 tcttgcaaga agtcgtgcaa gtttttcagt gcatccagca aggcatgctg ttcctccgta
    24121 gccattccgt ctaagatgtt cttgaccatt tcacgatgaa aatgctgatg gacccgaaat
    24181 agtaactttc cttttttcgt tagtcccaac tttacaacgc ggcggtcatc ttcactgcgg
    24241 atacgttcga catagccttt ttttactaac ttgttaatag cagtggtcaa ggtcccaaca
    24301 gtaattgata gttctttggc tacttctgaa gttgtttttt tcttgtacat cccgattgcc
    24361 tcgatcgtgt gcatctctgt gattgacaga tctttgaatt gagatttttt cagctcagac
    24421 tcttcgattg tcaaaatatc attgaagaca ctaacaagat aatcatttac tgtttttaaa
    24481 tctggttcca tgttccaccg tccactaatt agtttgatta tcaaatcttt tgatgttcaa
    24541 actatatctt aacttttttc aaaatgcaac cttaaaagat cattttttta ctatatgagc
    24601 tttttttaag attttactca tttaaaacaa ctgataaatc agcatttata atttctttta
    24661 ttagaaaaat catctatctc acatattttg attatcaaag tattttctgt gaacagaact
    24721 atagaattcc ctaaaacttg tccattattt caaaatttgt tatagtcttt ttattcaaag
    24781 cgtaaaagga gatcctatgt taaaagaaaa aatgaaaact ctccccatgc tgccgggtgt
    24841 ctatcttatg aaagaccgag atggtaaggt catctatgtt ggtaaagcaa aacggctcaa
    24901 aaatagagta agcagctatt ttcatcagaa taaacagcat tcaaaaaaag tattacgtat
    24961 gattcatcat ataacagatt ttgatttcgt cgtagtagac acggaattag atgctttatt
    25021 gctcgagtgt caattgatcc aacattaccg tcctttttat aaccgacaaa tgaattattt
    25081 cagtaattat aattatgtac atattaccaa taagggattt gtattaacag atactcctac
    25141 ggctcgaaca tacgggcctt ttcgtctcta taaaaaaatg ccgtctattt tgcgtataat
    25201 ggaagaaact tatcaaatgc cttggttgtc tgaaattagt ttgttagctt tgcgagtcca
    25261 gcttcctgac ctacaggaaa tgacttttga acagaaaaaa aaggaactcc aagggctgtt
    25321 tcaaggccga aataaaaagc tactgactta cttgaaaaaa agacaacaac atttcatcta
    25381 tcagttgaat ttcgaaaaag ccggtatgct tcaaaaagat atcgaattag ttacttattt
    25441 tattagacgg atccaggaac aaaagcagtt tttaagaaca ccatcgctca ccttcagcat
    25501 gccattagca gcagatgaga gtcagaaaaa gcactacctc gtttgttatg gtcaacttgc
    25561 agaaacaatg attacctctg gtgacgtttc tcctgacttc tattatgaaa agaaagaagc
    25621 tcatttatct ttgaaacgtc aattgtcaaa agaagaaatc gatcctgtac aaatcttgat
    25681 cagttaccgc aaaaaattag aaaaagaaca gtttgaaatt gaactgttaa acaaaaaaga
    25741 agctgaaaag caactaaatt agttgcttct cggcttcttt taggcatctt caacttcaaa
    25801 tgtcaattat cctaaaattt attggattaa gtcatagatt tccatcgcta cgatatcgat
    25861 attatcaaat tcgtattgct gatgagtatc tgtttcagtt aattcaaatg tatcggtcgc
    25921 tttatcataa gttactgtac acttctctac gccttctttt tcaaaacggc gaacttgcag
    25981 ttcattatct gctgactcga ccatagcctc cagtcgtttt atgattgcga ctaattgtga
    26041 atgtgtcatc ggaggtcctc cttttcttat ttctgttata ttgtatcaaa agcatagcct
    26101 acttgcattg attttctcta aaaaaactcg ttttttatga ttttttttct ggtatttttc
    26161 cttcaggcca ccaaagtttg atcaaagctt gggttccgtc attctcaaaa cctttatctg
    26221 ccaactcttc atatagtttc aatgcttggg tagttgctgg caatacaaga tccatttttt
    26281 ctgcttcttc taatgcaatc tttaaatctt tgataaaatg tttgacaaaa aaaccaggtg
    26341 tgtaatcttc ttttaaaata cgaggagaat aattgcttaa agaccagtta gctgcacttc
    26401 cacctgaaag cgtctcaatc actttcttta aatcaagtcc gtttttttgt gcatagacaa
    26461 gcatttccgt cattcctgtc atcgtacctg caatcatgat ctgattagcc atttttgtat
    26521 gctgaccttt cccagcagaa ccatgcaatg taaatgtcgt accaaattct ttgaataaag
    26581 gcagtacttg atcataaacg gcttcttctc caccgaccat gatcgtcagt gtaccattct
    26641 ttgctcctaa gtcaccaccg gataccggtg catctaacgc atctgcgcct ttttcttttg
    26701 ctgtttttgc aattttttct gccaggcttg gtgtacttgt agtcaaatca actaaaatat
    26761 gtcctgagat atctgcagaa aaaatccccg actgtccata gtagatttct tctacatctt
    26821 tggggtaacc taccattgta aagagaatat tactttgttc tgctaccgct tttggtgtat
    26881 ctgcccagat agctccttct gttactaaag gatcagcttt tgcttttgtc cgattataga
    26941 cagctacttc gtgtcctgct tctagtagat ggcgtgcgac agcacttccc atgacgcctg
    27001 ttccgataaa accaagtttc attttttcga cctcctaaaa atcaaaaatc cttgcttcta
    27061 tgctagtata cgctagaaac aaggattttt tcataaaaaa tgtttgaatt ttatctttta
    27121 aatgaattta attttatttt gattcgttca atcgagtctt cttcttcttt tcttcgcatc
    27181 tcttcttcat gataaacaac catgtcttga taaaattcga tcatagcttc gccgaaatgt
    27241 gtagatgaaa tatcataaag tttagcttct aaaacttttg gatctttagg aacttcactg
    27301 gaaatataat caataagtgc tggtgcaaag tcttcgtcgg tttcaaaagt tttccctaat
    27361 gaaggatgat cgaacaatcg gttcaaataa gcatttcctt ccacgacaca aggtgttccg
    27421 gctgccatcg cttctgtata agtaagccct tgtgtttctg aagtagaagc actgacaaaa
    27481 taatctgccg ctttataata aagtgctaca tcattgttgt ccacttctcc tgtgaactgc
    27541 acaaattctt ctaactgcag ttctgagatc aattcctcta actcttctac ataaggtcct
    27601 tttcctacga taactaatct ggcgttagga taagcggaga tgacctgagg aaacccatta
    27661 atcaaggctt ggatattttt ctcataagaa atcctgctta aagaaagaat catcaaatgg
    27721 ttttcctgaa gacctagtga ctcccttaat ttgctgatat cctgctgagt gatatcagaa
    27781 cgtttgaatt tttcaatgtc gataccagta ggaatcacgc gaagcggtgc tattacacca
    27841 tagctgcgta atgtatcaat aacacgttca cttggacaaa ctacacctgt cgtatgattt
    27901 gcaaataatc tagacaagta ttttacatgg gattgcctga tcacttttcc tttaccgata
    27961 taatgaaggt agtcttcata catcgtatgg taagtatgga tcagaggtat tttcaacttc
    28021 ttagctacca tcttcccaag tatccctgct ccgaattctg tatgggtatg aataagatct
    28081 agttctagtt ctttggcaac caaataagca taccacatgc ctctaacaac aactcttcga
    28141 tccttgaaag aaataaatgg tacacttggc attcgaatca catctttttc caacttatcc
    28201 gcattaggat cggtcgttgt aaaaatatag acttcatgcc catgtctttc taattcttgt
    28261 tttaatgttt tgatcgatgt agcaactccg ctgacttgcg gaaagtaggt atcggtaaaa
    28321 aagccgattt tcatgttttt cgcctccaaa ctagtccttg acctgattta ctttttttaa
    28381 tttgctagag actttttcat tcagtacact ttcgtataca tttttaagtt cttcaccgat
    28441 tttattgata cttcgctctt ttgctgtttg ataaccggct tgtgtcgtac tcggtagttc
    28501 ttgattggta atctgttgga tcagtcggat aaattcttcg tttgtcttcc ccatatagca
    28561 gttcttgcta tcttctaacc agccattata cactggaata tctcgaacaa gtacgttttg
    28621 tttgcttgct aaagcttcta aaacaacgat cccttctgtc tcttcataag aagggaaaaa
    28681 gaatagatct gcacctgaat acgcaccttc tatgatctct ccacgaatgt aaccaggaaa
    28741 caggacattt tcaggatgat cttcttttac aattttccgg atgttccgag gaatcgagta
    28801 catgggaaca tgaccaaacc aaataaatgt gtattcgggc atcttcttag caatttctac
    28861 gaaatcaatg attcctttac gttcgaaaaa caaaccgaca caaatgatta ctttttgatc
    28921 cggagacagt ttgaaatatt ctctgaactt ttgttctttc attggatcag ggtgatattt
    28981 ctctaagtcg atcccattgg aaatagcttg aatcggcaca cggataccat agctttccaa
    29041 caactgtttt gaataaggtg ttggcgtgat cagatagtcc gctttttggt acaatcctac
    29101 cagatatttt tttacgatag gagaaagctg gttcgatccg ataaaggaat ttcggaaatc
    29161 ttcctctgta gaatgcgcat gatagacaac tttttttcct tctcttctgg ctttattgac
    29221 catattatga ctattgattc cgtatgtatt gatgtgcagg atgtcataat cttctttttc
    29281 atctagtgta taagagatac cgacttccgt cagagcacgt ttttgatgat caagcgcacg
    29341 cccgattcca gattttgcaa gcatcttttc actttcaaaa tataataata ccttcacctt
    29401 acgtccccct tcacttatat agtataacat agcctacaaa ctgtcactca aacttaagcc
    29461 ctatttttgt ctattcttac tgacaaaaac gttttcttaa aaaagaacaa aaaaactggc
    29521 gtagctcttt tacaagccac accagctttt gatacgttgt ttattttaca tattcatgaa
    29581 caagttcaac gacttcttcc atcatatcgc attctttcaa tgcacgatca gcaagttcag
    29641 ccattttcgc tgtgtccaag cgtttcatta aactacgcgt tttaagaata gatgtcgcac
    29701 tcattgagaa ctcatctaga cccataccta ctagcaatgg aacagctgtt tgatctccag
    29761 ccatttctcc acacatacct gcccattttc cttcagcgtg tgctgcatca attacatttt
    29821 tgatcaaacg taaaattgat gggttatatg gttggtacaa gtaagaaacg cgttcgttca
    29881 tacggtctgc tgccattgtg tattgaatca aatcgttcgt acctacagag aagaaatcta
    29941 cttcttttgc aaacttgtct gctaagactg ctgctgctgg aatctcgatc atgatgccga
    30001 cttggatcgt atcagatact tccttgcctt cacttatcaa tttttgtttt tcttcttcaa
    30061 agatcgcttt tgctgcgcgg aattctttga gtgtagctac catagggaac atgatacgta
    30121 aattaccatg aacagatgca cgaagcaatg cacgcatttg tgtacggaac atttcgtcgc
    30181 cttgttcaga taaactgata cgcaatgcac gatatcctaa gaatggattc atttcatgag
    30241 gtaattgcag atatggtagt tctttatctc caccgatgtc cattgtacga acaactacag
    30301 gttttccttc cataccttct aatacagctt tgtaagcttc atattggtca tcttctgttg
    30361 ggaagtctgg agaatccata tacaggaatt cagtacggta cagaccaact gcttctccac
    30421 cattattatg gacaccaact agatcttttg gtgttccgat gtttgcagct aattcaaaat
    30481 gcttgccgtc agctgtgaca gtttctgcat tttttaattt ttcccattct gctttttgtg
    30541 ctgcgtaatc tgcacctgct ttttcaaatt cagctttttg ttcatctgta ggatcgatga
    30601 ttacatcgcc ttcgatcccg ttgacagcca aaatatcgcc ttctttgact ttagcagtaa
    30661 tttcttttgt cccaacgatt gctggaattt ctaaagaacg tgccatgata gcagaatgtg
    30721 aagtacgtcc accaatgtct gttacaaacg cttttacaaa attgcgatct aattgtgctg
    30781 tatcacttgg agtcaagtca tgtgcaacaa cgaccacttc ttcgttgatc attgatggat
    30841 ttggtaaagt aacgcctaat aagtgcgcta aaatacgttt agcaacgtca cgaatatctg
    30901 cagcacgctc ttgcatataa gcattatctt ccattgcttc gaacataccg atatacatat
    30961 ccgttacttc ttttagcgct gattcagcat ttacgctgtt gtctttaata ttttgcttga
    31021 tttgacctac catttctgga tctgaaagaa ccatcaaatg ggcatcaaat acttgcgctt
    31081 ctgcttcacc taagctttgc gctgcttttt cacgaatttg ctgcaattct tcagtagatt
    31141 tagctaatgc agcatccaaa cgagcttctt cagccgcagt atcttctact gttgccttac
    31201 tgaatgttaa atccggttga actagcaggt aagcttttgc aacggctaca ccgtcactag
    31261 cggcaatccc ttttagcatt tcaaccatta ttcagataag ccttctttct tcattgtttc
    31321 aacgattgct gccattgcat cagcttcgtc agcaccttct gcagtaatag taacatcaga
    31381 accttgacct acacctaaag acataacgcc catgattgat ttcaagttta ctgatttacc
    31441 tttatactct aagtttacat cagagttaaa tttgcttgct gtttgtacta atagtgtagc
    31501 tggacgagcg tggatcccag tttctgctac tacgtgaaat tctttctttt ccatattaat
    31561 cggtctcctt taagtgagtg ttgtttttat gtgagggttt tcttttttag taattcagag
    31621 aatcctaaca aatgccaccc tttcatctgt aaagattaac attttttttc gttagataca
    31681 acagttttca ttcaatttca gataaaagta ttcaaattat tccggattct ttttcatcct
    31741 tttcttcaca ttttataaca aacgtgttta tttccttttt cttgtcgctt tctcttctcc
    31801 tgacgtttat cattctttcc tttgaccctc tttgttttca tatgaaacga attacttgct
    31861 tcccttttta tttttagtat aataagagtc aaaggtcagg aatggtcaat agagatttca
    31921 ccttttctac ctcataaatc tcttaaaaaa ctaggggtta ttcttttacc tcatgaaaaa
    31981 cgagatgtaa aatatggatg tacccatttt tgtgaaagga cgtgaatgat tcatgctttg
    32041 tcaaaactgc ggcaagaatg aggcaacgat tcacttgtat gccactgtca atggtcagcg
    32101 gacacaactc gattactgtc agagctgtta tcaaaaaatc aaaaatcaac aaaatggagg
    32161 tctgactaat atggcccaaa atgatccatt tggatttggc agtttggacg atcttttccg
    32221 ttccatgtct cgccaaatgc aacagcaagg aaattatgag caaacaccac caacacaatt
    32281 tggcggaaac aacaacttca atggtggtca gccacctcaa ggcaatacag aaggtttatt
    32341 gggagaatac ggaatcaata tcacagaaca agcacgacaa ggtgatatcg atcctgtgat
    32401 cggacgcgat gacgaaatca aacgagtcat tgaaatttta aatcgccgaa ccaagaacaa
    32461 tcctgtcttg attggagaac caggtgttgg taaaacagca gttgttgaag gtttagcaca
    32521 aaaaatcgta gatggtgatg taccgcaaaa attaatggac aaagaagtca ttcgtttaga
    32581 tgtcgtatct ttagtccaag gtactggtat acgaggacaa tttgaagaac gtatgcaaaa
    32641 attgattgaa gagatccgtc aagcagaaaa tgtgatccta ttcatagatg aagttcacga
    32701 aatcgtcggt gctggttctg ctggtgatgg aaatatggat gctggtaata ttttaaaacc
    32761 agctttagct cgaggagaat tgcaaatggt tggtgctact accttaaatg agtaccgcat
    32821 catcgaaaaa gacgctgctt tagaacgtcg tatgcaacct gtacgggtag atgaaccaac
    32881 tgtcgaagaa acgatcagta ttttgaaagg tttgcaaaaa cgttatgaag actatcatca
    32941 tgtaaaatat actgatgagg ccatcgaagc agccgcaact ttatccaatc gttacgtcca
    33001 agatcgcttc ttgccggata aagccatcga tttgctagat gaaacaggtt ctaagaaaaa
    33061 cttgacgatc caaatcgttg atcctaaaac aatcgagaaa aaactgcaag aagcagaaga
    33121 acaaaaagtt ttagcttctc gagaagaaga tttcgaaaaa gcagcttatt atcgcgatca
    33181 gatcaataaa cttcaaaaaa tgaaagaacg tcagctcact gaagaggaaa cacctgtgat
    33241 taccgaaaaa gatatggaaa aaatcgttga acaacggaca ggtattccag tgggtgaatt
    33301 aaaagaaaaa gaacagacac aactgaaaaa cttagcagat gacttgaaag ctcatgtcat
    33361 cggacaagat aacgctgtag atcgagtagc gaaagcgatt cgccgaaacc gtgttggatt
    33421 aaacaaacag aatcgtccaa tcggctcctt cttatttgtt ggaccaactg gtgtaggtaa
    33481 aacagagctt gcgaaacaac ttgcctacga actattcggt tcacaagatt caatgattcg
    33541 tttcgatatg tctgaatata tggaaaaaca tagtgtctct aaattgattg gttctcctcc
    33601 aggttatgtc ggatacgaag aagctggtca attaacagaa aaagtacgga gaaatcctta
    33661 cagtcttgtc ttgttagatg aagtagaaaa agctcatcca gatgttttgc atatgttctt
    33721 gcaaatctta gatgatggtc gattgacaga tgcacaagga agaacggtca gctttaaaga
    33781 tacgatcatc attatgacaa gtaatgccgg aactggtaaa gtagaagcaa acgttggttt
    33841 tggtgcggca cgcgaaggtg ttactcgttc tgtattgaac caattaaaca actactttac
    33901 accagaattt ctgaaccgtt ttgacgggat catcgaattc agtgcattat caaaagaaaa
    33961 cttgatgaca atcgtcacac taatgctaga cgatgtcaac caaatgctgg cagcacagca
    34021 acttcacatc gaagttccta cgaatgtcaa agaaaaatta gttgacttag gctatgatcc
    34081 atctatgggt gctcgtcctc ttcgacgcac gatccaagaa caaatcgaag atggtatcgc
    34141 tgaattttat cttgatcacc cttctatcca tgagttgaaa gcaaaactgg ataaagatgg
    34201 aaaaatcatc gttacttcta aaccagaacg tttggctaaa gaatcagccg aagaaacggc
    34261 tgaataaatc cagttttata gcgtgagacg gtatttcttt gagaaatgcc gtctcattct
    34321 tttttcagaa aagtcaaaat actgttcatt ttataaaaaa gaaattataa taagagaaac
    34381 aagcaattaa aaatacataa aagaggtgag cgccatgaaa ttggtaaatg taacaaacag
    34441 ccattcccgt cttgtattaa accagcttga aaacactgat gcccatctag tcaaggtcta
    34501 cacagccgga aataccacta ttgtctacac tgaagcccct gaacataatg aaattctgct
    34561 gatcaatgac aaacgtaaaa tccagccgaa agaaatcgaa gatgctaaaa attatttctt
    34621 agataaaatc aaagacgccg tttaccacga ggatgaaatc aaagtaatcg aattggcagg
    34681 ccttgttgaa atctcgattc caaaacatga attaggcgct acattagccg tgtaaacaaa
    34741 ttttgaacat aataattaaa gagcgtggga caaaagtaaa gaacactttt gtctcacgct
    34801 ctaaacacgt ataaacggcg aaagcagaag caactccttc ggaaataagc tgaaattcac
    34861 gaaaattgct tttcaaattt ttgtgaattc cagcttattt cttggagtta aacgcttctg
    34921 tctcagcctc tttttctttc tagctttttg atttttctct tttcttttgt acttttctct
    34981 tttcattttc agttctacgc gtgtctttat ttttaacttt ttcctcttta tggatgattg
    35041 atcgtacttt ttgtgcaatt tgctcgtttt tgtcccagtc ttttacatct aataatcgac
    35101 ataatcgtaa catctcttct tttttctgtt tttttctttc acgtctttgc cattctgttt
    35161 ccatgtgtca cactctcctt tctttaatca tatgtacgca aaataatttt tatatttttt
    35221 tgattagaca tttctatgat tttaatatag taaaaagagc cgtc
//
LOCUS       DACDIH010000023        34413 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDIH010000023 DACDIH010000000
VERSION     DACDIH010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.023"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            212..604
                     /locus_tag="IV281_001199"
     CDS             212..604
                     /locus_tag="IV281_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8780225.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="IV281_001200"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="IV281_001200"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP8780226.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="IV281_001201"
     CDS             3165..4775
                     /locus_tag="IV281_001201"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP8780227.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="IV281_001202"
     CDS             5067..5936
                     /locus_tag="IV281_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP8780228.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="IV281_001203"
     CDS             6101..7372
                     /locus_tag="IV281_001203"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8780229.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /locus_tag="IV281_001204"
     CDS             7369..8664
                     /locus_tag="IV281_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP8780230.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="IV281_001205"
     CDS             8763..9026
                     /locus_tag="IV281_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP8780231.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="IV281_001206"
     CDS             9471..9920
                     /locus_tag="IV281_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8780232.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="IV281_001207"
     CDS             9977..11395
                     /locus_tag="IV281_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8780233.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="IV281_001208"
     CDS             11395..12255
                     /locus_tag="IV281_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP8780234.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="IV281_001209"
     CDS             12429..13202
                     /locus_tag="IV281_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8780235.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="IV281_001210"
     CDS             13349..13714
                     /locus_tag="IV281_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP8780236.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="IV281_001211"
     CDS             complement(13862..15070)
                     /locus_tag="IV281_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8780237.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="IV281_001212"
     CDS             complement(15089..15367)
                     /locus_tag="IV281_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780238.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="IV281_001213"
     CDS             15560..16216
                     /locus_tag="IV281_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8780239.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="IV281_001214"
     CDS             complement(16658..17455)
                     /locus_tag="IV281_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP8780240.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="IV281_001215"
     CDS             complement(17452..18636)
                     /locus_tag="IV281_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8780241.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="IV281_001216"
     CDS             complement(18661..19908)
                     /locus_tag="IV281_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP8780242.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="IV281_001217"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="IV281_001217"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP8780243.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="IV281_001218"
     CDS             21888..22202
                     /locus_tag="IV281_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP8780244.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="IV281_001219"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="IV281_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP8780245.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="IV281_001220"
     CDS             22887..23531
                     /locus_tag="IV281_001220"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP8780246.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="IV281_001221"
     CDS             23546..23875
                     /locus_tag="IV281_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780247.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="IV281_001222"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="IV281_001222"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP8780248.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="IV281_001223"
     CDS             24863..25687
                     /locus_tag="IV281_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP8780249.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="IV281_001224"
     CDS             25680..26027
                     /locus_tag="IV281_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP8780250.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="IV281_001225"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="IV281_001225"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP8780251.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="IV281_001226"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="IV281_001226"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP8780252.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IV281_001227"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IV281_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP8780253.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IV281_001228"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IV281_001228"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP8780254.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="IV281_001229"
     CDS             31684..32451
                     /locus_tag="IV281_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8780255.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..>34413
                     /locus_tag="IV281_001230"
     CDS             32441..>34413
                     /locus_tag="IV281_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8780256.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSG"
BASE COUNT        11062 a         6108 c         7231 g        10012 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg ctt
//
LOCUS       DACDIH010000024        32747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDIH010000024 DACDIH010000000
VERSION     DACDIH010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.024"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            188..418
                     /locus_tag="IV281_001231"
     CDS             188..418
                     /locus_tag="IV281_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP8780257.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            515..1816
                     /gene="murA"
                     /locus_tag="IV281_001232"
     CDS             515..1816
                     /gene="murA"
                     /locus_tag="IV281_001232"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8780258.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1829..2005
                     /locus_tag="IV281_001233"
     CDS             1829..2005
                     /locus_tag="IV281_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8780259.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2140..2436
                     /gene="yidD"
                     /locus_tag="IV281_001234"
     CDS             2140..2436
                     /gene="yidD"
                     /locus_tag="IV281_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP8780260.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2621..3088
                     /locus_tag="IV281_001235"
     CDS             2621..3088
                     /locus_tag="IV281_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8780261.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3173..4057
                     /locus_tag="IV281_001236"
     CDS             3173..4057
                     /locus_tag="IV281_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8780262.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4174..4407
                     /locus_tag="IV281_001237"
     CDS             4174..4407
                     /locus_tag="IV281_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP8780263.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4495..4575
                     /locus_tag="IV281_001238"
     tRNA            4495..4575
                     /locus_tag="IV281_001238"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4529..4531,aa:Tyr,seq:gta)
     gene            4582..4653
                     /locus_tag="IV281_001239"
     tRNA            4582..4653
                     /locus_tag="IV281_001239"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4614..4616,aa:Gln,seq:ttg)
     gene            5168..5875
                     /locus_tag="IV281_001240"
     CDS             5168..5875
                     /locus_tag="IV281_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8780264.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5934..6578)
                     /locus_tag="IV281_001241"
     CDS             complement(5934..6578)
                     /locus_tag="IV281_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP8780265.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6704..6991
                     /locus_tag="IV281_001242"
     CDS             6704..6991
                     /locus_tag="IV281_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780266.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7099..7419
                     /locus_tag="IV281_001243"
     CDS             7099..7419
                     /locus_tag="IV281_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8780267.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7500..8813
                     /locus_tag="IV281_001244"
     CDS             7500..8813
                     /locus_tag="IV281_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8780268.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8810..9529
                     /locus_tag="IV281_001245"
     CDS             8810..9529
                     /locus_tag="IV281_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP8780269.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9657..10208
                     /gene="raiA"
                     /locus_tag="IV281_001246"
     CDS             9657..10208
                     /gene="raiA"
                     /locus_tag="IV281_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP8780270.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10430..12964
                     /gene="secA"
                     /locus_tag="IV281_001247"
     CDS             10430..12964
                     /gene="secA"
                     /locus_tag="IV281_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP8780271.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13090..14191
                     /gene="prfB"
                     /locus_tag="IV281_001248"
     CDS             join(13090..13152,13154..14191)
                     /gene="prfB"
                     /locus_tag="IV281_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP8780272.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14296..14982
                     /gene="ftsE"
                     /locus_tag="IV281_001249"
     CDS             14296..14982
                     /gene="ftsE"
                     /locus_tag="IV281_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP8780273.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14966..15859
                     /locus_tag="IV281_001250"
     CDS             14966..15859
                     /locus_tag="IV281_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8780274.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            16011..17459
                     /gene="cls"
                     /locus_tag="IV281_001251"
     CDS             16011..17459
                     /gene="cls"
                     /locus_tag="IV281_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8780275.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17796..18653
                     /locus_tag="IV281_001252"
     CDS             17796..18653
                     /locus_tag="IV281_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP8780276.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18768..19688
                     /gene="pstC"
                     /locus_tag="IV281_001253"
     CDS             18768..19688
                     /gene="pstC"
                     /locus_tag="IV281_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP8780277.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19688..20572
                     /gene="pstA"
                     /locus_tag="IV281_001254"
     CDS             19688..20572
                     /gene="pstA"
                     /locus_tag="IV281_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP8780278.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVSEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20587..21393
                     /gene="pstB"
                     /locus_tag="IV281_001255"
     CDS             20587..21393
                     /gene="pstB"
                     /locus_tag="IV281_001255"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8780279.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21409..22167
                     /locus_tag="IV281_001256"
     CDS             21409..22167
                     /locus_tag="IV281_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8780280.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKNKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22180..22857
                     /gene="phoU"
                     /locus_tag="IV281_001257"
     CDS             22180..22857
                     /gene="phoU"
                     /locus_tag="IV281_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP8780281.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23094..24125
                     /locus_tag="IV281_001258"
     CDS             23094..24125
                     /locus_tag="IV281_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8780282.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTNQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24122..24889
                     /locus_tag="IV281_001259"
     CDS             24122..24889
                     /locus_tag="IV281_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8780283.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24989..26557
                     /gene="liaX"
                     /locus_tag="IV281_001260"
     CDS             24989..26557
                     /gene="liaX"
                     /locus_tag="IV281_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP8780284.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26857..27174
                     /locus_tag="IV281_001261"
     CDS             26857..27174
                     /locus_tag="IV281_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP8780285.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            27174..27524
                     /locus_tag="IV281_001262"
     CDS             27174..27524
                     /locus_tag="IV281_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP8780286.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27691..28629
                     /locus_tag="IV281_001263"
     CDS             27691..28629
                     /locus_tag="IV281_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP8780287.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28644..29474
                     /locus_tag="IV281_001264"
     CDS             28644..29474
                     /locus_tag="IV281_001264"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP8780288.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29518..30543
                     /locus_tag="IV281_001265"
     CDS             29518..30543
                     /locus_tag="IV281_001265"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8780289.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHCLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPVEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30558..31496
                     /gene="galU"
                     /locus_tag="IV281_001266"
     CDS             30558..31496
                     /gene="galU"
                     /locus_tag="IV281_001266"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8780290.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31621..32061
                     /locus_tag="IV281_001267"
     CDS             31621..32061
                     /locus_tag="IV281_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP8780291.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32064..32666
                     /locus_tag="IV281_001268"
     CDS             32064..32666
                     /locus_tag="IV281_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8780292.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
BASE COUNT        10867 a         5552 c         7097 g         9231 t
ORIGIN      
        1 tttggaataa gggactgcga caagactttt gtctcagtcc ctttttttgc atatttttag
       61 catttatttc ctaaaaaatg taaggggatt cctatatttt taagtatact tgtttttcct
      121 tttatacatt ttatggtatt atcattttat tagttattat aaaacaatcg ttagaaagga
      181 cgtcattatg caagtatacg gaattgatgc ggtgcttcga atcgtgagtc atttggcgtt
      241 tatctactta gccttctggt ctttacgttc gttacgcatt gaaacttttt tcagaccaat
      301 gcataatacg caaattagaa tggcaatcgt acttttttca atttttttag ggtacactgc
      361 aagtaatttt ttcttagaag ttatcgcttt atgccgaaat cttttcgtta gcctgtaaat
      421 tttaaaaaaa ggtactattt taaaagcaag gatgttataa tagaaaagat tttgagtaag
      481 tgtatgctca ttggaatttt ttggagggaa caacatggaa gagatcatcg taagaggtgg
      541 caatcaatta aatggaacag taagaattga aggagctaaa aatgctgttt tgcctatttt
      601 agctgctagt ttgttagcag aagaaggtat tacgacatta gacaacgttc cgattctttc
      661 tgatgtcttc actatgaatc aagtgattcg tcacttaaac gtagatgtag attttgatga
      721 acaaaagaat caagtaacaa tcgacgcttc aagacaatta gaaatcgaag cgccttatga
      781 gtatgttagt caaatgcgtg cttcaatcgt tgtgatgggt ccgctacttg ccagaaatgg
      841 acatgcaaaa gtagctatgc ctggtggatg tgcgattggt aaacgaccaa tcgatttgca
      901 tttaaaagga tttcaagctt taggagcaaa aattattcaa aaaaacggct atatcgaagc
      961 aattgcggat gaattgatcg gtaacacgat ttatttagat ttccctagtg taggtgctac
     1021 tcaaaatatc atgatggctg ccgtaaaagc aaaaggaaca accatcattg aaaacgtagc
     1081 tcgcgaacca gaaatcgttg atttagcaaa cattttgaat aaaatgggcg ctcaagtcta
     1141 tggagctggt actgaaacaa tgcgtattga aggcgtggat catttacatg cagtcaacca
     1201 ctcaatcgta caagatcgta tcgaagcagg tacctttatg gttgctgctg caatgacaca
     1261 aggcaatgtg ctgattgcag atgctatctc tgaacataac cgcccattga tttctaaatt
     1321 gatcgaaatg ggtgcagaaa tcattgaaga agaaggcgga gtccgtgtca tcggaccaaa
     1381 acatatctta cctacagatg taaaaacgat gcctcatcct ggtttcccta cagatatgca
     1441 agctcaaatg acagctatcc aattagttgc agaaggtaca agtgtagtga ctgaaacagt
     1501 gtttgaaaat cgtttccaac atttagaaga gatgcgccgt atgaacgctc atgtcaaaat
     1561 cgacggaaat gtagcaatca tggatggaaa ccatgaattg caaggtgcag aagtatatgc
     1621 aacagacttg cgggcagcag cagcattagt tttagctggc ttgaaagcaa atggaatcac
     1681 acgtgtgcgt aacttgaatt atctagaccg tggatattat aatttccata tcaaactaca
     1741 gcaattaggt gcagacgttg aacgtgtaga catggatcaa acttctgctg aaaaaacagc
     1801 acaaacgatt gcttaaggaa gtggctgaat gagtaaccaa cgatatatct tattaacttt
     1861 gataaagatt ttaatggtga tcttattgct tatccttctt tttgtggccg gaacgatgat
     1921 cggatatggt gtcatcggtg gcggcaatcc atttaaagta tttcagccaa gtttgtggat
     1981 tcatattcga gacttttttc attgataaat aagaatcaca gtggttctag tcgatatgaa
     2041 gtgagccatg aagcagaaca tgataaaaga aaacagaagg gaacgttgac gttagtcgcg
     2101 atcccttttt ttagtaaaag aaaagaagga atctaattga tgaagaagat attgatttat
     2161 ttagttagag gatatcagcg ctttatttcg cctgcatttc cacctagctg ccgttattac
     2221 ccaacctgtt ccaattatat gataaaagca attcaaaccc atggcgcgct aaaagggacg
     2281 gtaatgggga ctgcacggat cttacgctgt catccttttg tcaaaggcgg tatcgattat
     2341 gtaccattga cattccgatt aacgcgaaat aagaaagata aaaaagaacc agattataaa
     2401 aaggttgtga aaaatacggg tagcactgag cgataaagaa acaatttgga aaatagcttc
     2461 ttatattttg caaaattgtg agttattgcc gaagtgttgg attttgaaca ccgtcaaaaa
     2521 aaaggttgtg aaaaatacgg ctagcactga gcgataaaga aaaagaggaa atttattgtc
     2581 aaagtaacaa ctccgcaact acttatatgg gggacaaaaa atgatcgaag aactaaacaa
     2641 gaaagattta ccatggtcct tgttattaga tgcagatcct gataaagaaa aagttcaagt
     2701 atatgtttcc agagggagtg gctggatatg gaaagaaaaa gaaaaaacaa taggtgtact
     2761 gatctatgtg gcaagggaaa aagaatacga aatcgtcaat gtagcagtaa ccccaacgta
     2821 tcaaggaaag gggattggcg gaaaattact tgagacagct tttcagaagc tttcacaact
     2881 cgcttccagt caaactcaga tcatcatacg aaccggcagt actagcagtg cggcgttaca
     2941 tctctatcaa aaaatgggct atgtggagat tggtcgagtc aaagactatt ttatccatca
     3001 ttactctgag cccatttttg aaaatggaga tcaattaagg gatcaagtga ttttagcaat
     3061 tcacttacct aaaaatagct ttaactaata aaatttcaat tcaagaaaat gctttcaaat
     3121 gctaaataga tacattacaa tagctttagc aaacaaaaag gagaatattt ttatgggaat
     3181 attagttttt gattttggtg gatcggctgt caaatatggt tgttgggatg gcaaagagat
     3241 taaaggtaag ggacaatttg caacgcctga atcttgggaa gaaatgaaag cgcaattatt
     3301 tcaagtttat aaaaaaatac tcgtttcttt tgaagtcgaa ggaattggaa tcagctctcc
     3361 tggagtagtg aataatgaga aacaggtgat tgagggaatc agtgcgattc cttatattca
     3421 tggatttaat attttccgag atttggaaac tctgtttcaa cttccagtaa cgattgaaaa
     3481 tgacgccaac tgtgccggaa tggctgaatt ttatgaaggt gctgcccgcg attatcaaga
     3541 tgttgctttt gttgtggcag gaactgggat tggcggggcg ttgttcacga aaggacaaat
     3601 caataaaggg gcacatcttt acggtggaga atttgggttg atgttcttaa atggcgataa
     3661 gacattcagt aaacttggta cagcggtaca gatggcttgg cgttattgtg aacgcaaagg
     3721 tctcgataaa aatgcttata ccggaaaaga tgtctttgaa ttagcagaaa ctggagatgc
     3781 aatagcaaaa gaagaagttg agagttttta tacgtatctt acaaagggat tattcagtat
     3841 tcaattttcg tttgatccag aagtgatcgt attaggcggc ggggtttctg caaaagaagg
     3901 gctcattgat gagatcaaaa gccgaatgaa gaagctgaca gaacaattcg atctgcatga
     3961 tttcgaaccg cagattttat tatgtgaata cagaaacgat gctaatcttg taggtgcagc
     4021 agcaaactat ctcgctgtac gtcaagggga gaattaagag ctttagagaa ggtgcagctt
     4081 gcataaaaga agtgaatgaa atgaatagga gatttttacc taaggaatgt tttatttgct
     4141 gcagtatgat aaaatatctg agaggtgaaa atgatgtcaa aaaaaaataa agatattgaa
     4201 gtgcgtatag aagaagtgaa aaaagatatc aaaggtaaga attatgaagt cactcagtta
     4261 tttataggga aaaaaatgat tggtgagatc ttagcctatg gaccaaaaga atatgaaatt
     4321 ttctttggtg aagaagattt tggaaaagaa aaaagtcttg aaaatgcgat agaaacagtg
     4381 attcgacact ggaatctgca cgaataaaaa aatatcaaaa aaactttcaa taaagggttg
     4441 caaaaatctg gaaaattagg tattataact aagtcaggtt gatacaaacc tttcggagga
     4501 gtagcgaagt ggctaaacgc gacggactgt aaatccgttc cttagggttc agtggttcga
     4561 atccactctc ctccatttca ttggggtata gccaagcggt aaggcaaggg actttgactc
     4621 cctcatgcgt tggttcgaat ccagctaccc cagtatacca caatcgtaag ctctcgttag
     4681 ttcgagagct cttttttttg ctttgctacc ttgttccttg aagattctgc tgatttaaga
     4741 tagagttaag ttaactacaa gaattggagg gatccacatg aaatggaaag aaaaagtaga
     4801 cgaagcagct gaaaagttat atgatttggt caaatcagaa aaatacaata ttgaagtgaa
     4861 tattccgaaa aaaggcggaa aagcggtccg ggtaaaatca aaacgcccaa cgaatcatac
     4921 aaaaaaatgg atggctaaaa accgctaata ataacaaaag atccataagt cggcattttc
     4981 atcgatttta gggtcttttg ttattttcta ggttggacat atctaaaaaa ttgtttctga
     5041 caataggagt aggaagaacg atgtgtgtct aaatcctctg ttgatggata gaaaccttta
     5101 tctttttcag ttgtgaatca ggtatatacc agttataata gaagaaacga aaggacgggt
     5161 gtacgttatg tcgaatcaat tacctgtata tatacagatt catgaccaat taaaaaatga
     5221 aatcgaacaa ggtatatgga aagtcggaga ccgtcttcct tcagaaagag aattagcagt
     5281 gaaattcaat gtcagccgta tgacgttgag acaagcaatt caaaacttgg cagatgaagg
     5341 aatcttagaa agaaagatcg gttcgggtac atatgttgca cgagaaaaag tacaagaaaa
     5401 aatgtctggt gcaacaagtt ttacagaaat tatggaatca caaaatcgag taccgtctag
     5461 tcggacaatc tcttatttct tgacttcgcc aagctcaagt gaaatggaaa aactaaactt
     5521 gagtaaagat gacacgatct tgcgaatgga acgtattcgt ttcgcagatg atgtccctat
     5581 ttgttttgaa gttgccagtg tgccacaaaa gctgatcgat ggctacagca aagcagagat
     5641 tactgaatcg ttgtatcgca cgctagaaga aaaaggcggg aaaaagatcg gtccagcgaa
     5701 tcaaacgatt actgctgtgt tggcttctga gaaaattgct gactatttag aggtaaaacg
     5761 aggagatgca gttcttcgtt tgcgccagat ttcttatttt gaagacggta cgccgtttga
     5821 gtatgtgcgt acgcaatacg taggaaatcg ttttgagttt tatttggaaa aataaaaaac
     5881 aggtgatccc tgattccgta ttagaaataa aagagaatca gaatcacctg tttttatata
     5941 tgatcagtta ccagttgctc gtgataagaa acaggaccct cttcaaattg gacttgtccg
     6001 ttcagcagct caatgatgga ttctttaaag tggtcagcac ttttttcatc tacagcacag
     6061 atgacgctaa cttgatcagt atattggatt tcttttataa aaaaatgttg gagttcaaga
     6121 taattctgta attttcctag ttgcggatac gtgatcatca cagagatctc ctgttgcaag
     6181 gtaccttcta cgattccaat gtcagacaat gcttgagaga ccgcatgagt gtatgctcgt
     6241 attaatccgc ctgtgccgag tttcgttcct ccaaaatatc gagtgactac tgcagctaca
     6301 ttgatcagtt gatttttttt gaggacttcc aacatcggta tgcccgccgt tccacttggt
     6361 tctccatcat cgctgcttcg ctggatttca tttttttctc caatgatgaa ggcgctgcag
     6421 ttatggttcg ctttccaatg ttcttttttt atttcttgaa tgaaactttt tgcttcgtcc
     6481 tctgattcta ttcgtctaag agaacagata aagcgtgatt ttttgatctc gatctcactt
     6541 tgtccatttt cttttattgt acgatagcgc tcgatcatct tgtcacctct tttttcattt
     6601 tctttattca gtataaataa agttctaggt tgtttcaaca ctgtaacggt tgcgcaattg
     6661 ctgtagtatg cgataataaa gacaaaggca ggtggatctg gggatgcagg aacgaatgaa
     6721 aaagtacgat gcaattacgc attatttgaa gaacaatggc ggctctcaag taacgttgac
     6781 ctttacccaa tttgacgaac tgctgtttcc cagtaacgga cttcccaaaa cagcaaggga
     6841 aagtacagat tggtgggcaa atgattataa acaccctgaa aaaggtgcct atggctggat
     6901 aaatgcaggc tatgaagtag ttgtcatcaa tttggacaaa gaatatgtcg tgttcaataa
     6961 attagtgaaa tctagttggc tatttgatta aataaaccgc gtttagaaaa cgttgacaaa
     7021 tttctttctt tttatataat agagtaacag acgcgaagaa aacgtttgcg tagtttgttt
     7081 taaaaaggag agaaaagtat ggctaaaaaa acaatcatgc ttgtatgttc ggctggtatg
     7141 agcacaagtt tactagtaac aaaaatgcaa aaagctgctg aagcaaaagg aatcgactct
     7201 gatattttcg ctgtgtccgc ttctgacgca gataacaatc ttgcaaataa agacgtggat
     7261 gtgttgttat taggcccaca agttcgcttt atgaaagctg attttgagaa acgtctggaa
     7321 ccaaaaggca ttcctttaga tgtcatcaat atggctgatt atggtatgat gaatggcgaa
     7381 aaagtgttag atcaagcaat cacgctcata ggtgaataaa tagaacagta taactatcga
     7441 acaagcgatc caaaaaatag atcgcttgtt ttttcgtata tttttttagg gcgaataata
     7501 tgaatcattt ttggggtaga aggcttctga cgagagatat taaagcaata gactgtgagg
     7561 aggtaaattt accaaattat gagaagatcg cagcagtgga acgtttgagc aactggattc
     7621 tttgtcatcg ctgtggaatg agaacgtccg tcattgatgg acagcttgca gattacggtt
     7681 atttttgtac acattgtcta tatttagggc gatgcgacac tcaacaagat ttatatttgt
     7741 ttgatcagcc tgaagcaaaa cctcgagaag tcgtcttttt ttggacagga cagcttactg
     7801 aaaaacaaac ggaaatctca aagagaatac tttgccattc agaaaaaaga catcatctta
     7861 tttgggctgt aacaggtgca gggaaaacag agatgcttta tcctatttta gtggaaactt
     7921 taaaagctgg aggaagagta gcgatttgta caccgcgagt cgatgtttgt aatgaattgt
     7981 ttttacgcta tcgttatgtt tttccagaag aaaaaatcac gttgctccat gggaatacag
     8041 caacggctta tacctttact tcttttgtta tctgtacgat tcatcagcta taccgttttt
     8101 atcggtcctt tgatttaata gtgatcgatg agatcgatgc gtttccttat aacggggatg
     8161 caggattagc tcatgctgtg caaacagcga ttaaaccaga cggacgattc atctacttaa
     8221 gtgccacacc tgataaaaag ttactaaagg agatcaagca gacgtttgac atccacaaac
     8281 ttgctttacg ctttcatcgg cgtttgcttc ctgaacccgt cttggttttc atgaataatt
     8341 ggcgacaaaa atgcaagaac aagaagaaga taaaatcttt ggtgcggatc attcatcaat
     8401 taattaaaga aaaccatatc cttttatttt gtccaagtat cacgatgatg catcgtttga
     8461 aagccacact tcaagaggct cttccgagat atgagatcac agatgtttct tcaagagaca
     8521 atcagcgggc ggaaaaagtc cagaaaatgc gagaagcaaa atatgacata ctgttaacca
     8581 cgatgatcct tgaacgtgga gtcacatttg aaagaatatc cgttatcgtt ttaggagcgg
     8641 atcatccggt ttttactaaa tcttctttgg tacaaatcgc aggaagagcc gatcgtaaag
     8701 ggccttatac gaacagtcaa gtatattttt tctatgaaga aaaaactgca gctattcgaa
     8761 aagcttgtca ggaaatcaaa gaaatgaatg aagaagggaa gaagatactg tgaggtgcag
     8821 ttgctgcagt aaagatatcg tccaaaattt aacattcagc gagattattg gtgtttcgaa
     8881 atacagatta cctcagactt gttcagtttg tcgcgcacaa tttcatttat tggaaacaag
     8941 aggctgttct ggctgtcaaa aaccaatcca tttcaatcta gagagtacga aaaagaaagt
     9001 aaattctatc cccgtttact gcagtgactg tcaagaatgg aagaagaaat atccgaccta
     9061 tgattttcag caccgtgcct tttttgtgta cgatgaaaat atgaaggcct ggctcaatca
     9121 atacaagttt atgggagata tccgactagc aggcaccttt gcatataaat ggacgcagat
     9181 aaaaaaagaa tacccgacat acatctattg tccaatacct ttatctgaaa aaaggcgaac
     9241 agaaagagga ttcaatcaag taagcgagat gttgaaagca gcaagaattc cttttcagat
     9301 gctactcaaa agagagaagc atctgctggc tcaagcaaaa aacacgcgaa aggaaagatt
     9361 ggagatgcct caaccttttg aacttgcagt caccttttct aagatacaaa ataaaaaaat
     9421 attgattatt gatgatgtct atacaacagg acagactctt ttccacgcag cagattgttt
     9481 gcagatcgca cagccgaaaa tcatccgaac tatttctttg gcgagatgaa ttagatgtgg
     9541 aaatttgata gcgatttaat ttgcaaataa cctagagttc gatataatag aattaagaag
     9601 agggaaagag tggtccttct ccttcttgat tgtggtagac gaaaggggta atttttatgt
     9661 ttagatataa tgtacgcggc gaaaatatcg aagtaactga agcgattcgc gactacgttg
     9721 agaaaaaagt aggcaaacta gaacgctatt tcagtgacgc acccgatgca acggcgcatg
     9781 tgaacttaaa agtttataca gaaaaaacag caaaagtgga agtaacgatc ccattacctt
     9841 atcttgtatt acgtgccgaa gaaacttctc cagatttata cgcaagtatc gatttggttg
     9901 ttgacaaatt ggaacgccaa atccgcaaat tcaaaacaaa aattaatcgt aaatcccgtg
     9961 aaacgggcat gaatactgca aatgctgctg ctttattcaa tgaagaggac gaaaacgaaa
    10021 atgattctga attagatatc gtacgtacga aacgcctttc attgaaacca atggatagtg
    10081 aagaagcagt cttgcaaatg aacatgctgg gacataactt ctttattttt gaagatgcag
    10141 aaacaaatgg tacaagtatc gtttaccgcc gtaaagatgg aaaatacgga ctgattgaaa
    10201 cagactgatc agccttactt aaataacaac gaaaacttgc agcagctact gacagctgcc
    10261 gcaagttttt ttgagtttcc tcctctttta gatgaaatat gaataaatta tgaagttctt
    10321 actttaatac gcttaagata tttcaattat ctataactaa tgataaaatg aaaagtaaag
    10381 tctacatagt ctggataata cacagaagga aaaggagatt acacaacgca tggccaattt
    10441 gattcgttca cttatcgaaa acgataagaa agaactaaaa cgtttagata gcattgcaaa
    10501 aaaagttgaa gcatacgctg accaaatggc agcattaact gatgaacaat tacaggcaaa
    10561 aactgctgaa ttcaagggca gataccaaaa aggagaaaca ttagatcaat tacttccgga
    10621 agcttttgca gtcgtccgag aagcagcaaa acgtgtatta ggtttatatc cataccacgt
    10681 acaattgatg ggtggaatcg tgcttcacga tggaaatatc cctgaaatgc gtactggtga
    10741 aggtaaaacc ttgaccgcaa cgatgccagt atatctaaat gctctgactg gcgaaggggt
    10801 acacgtagtt accgtcaatg aatatttagc aacccgtgac tcgacggaaa tgggcgaact
    10861 ttataatttc ttaggattaa cagtggggtt gaatatcaac tcaaaatctt cggatgaaaa
    10921 acgcgaggct tataattgtg atatcactta tagtacgaat aatgaattag gatttgacta
    10981 cttgagagac aatatggtcg tttaccgcca tcaaatggta caacgtccgc tgaattatgc
    11041 cattgtcgat gaagttgact caatcttgat tgatgaagcg agaacgccgc tgatcatttc
    11101 agggcaagca gaaaaatcca cagctttata tacaagagca gacaacttcg tgaaacgttt
    11161 gaaagaagaa gaagattata aaatcgatgt acaatcaaaa acaatcagtt taaccgaagc
    11221 agggatcgaa aaagcagaag aaacattcgg tttggataac ttatacgaca ttgaaaatac
    11281 tgcattgacc catcacttgg atcaagcatt gagagccaac tatatcatgc ttttagatat
    11341 cgattatgtt gtccaagacg gaaaagtact gattgttgac cagttcactg gtcggatcat
    11401 ggatggacgc cgttattcag atggacttca ccaagcaatc gaagctaaag aaggcgtaga
    11461 gatcgaagac gaaacgaaaa ccatggcgac gatcactttc cagaactatt tccgtatgta
    11521 caagaaactt gcgggaatga ctggtacagc taaaacagaa gaagaagaat tcagagaaat
    11581 ttataacatc caagtatacc agattccgac aaatcgtccg gttatccgtg atgatcgtcc
    11641 agatttgctt tacccaacat tacaaagcaa gttccacgct gtcgtgcaag atatcaaaga
    11701 tcgctaccgc aaaggtcaac ctgtgttagt cggaactgtt gcggttgaaa catctgagtt
    11761 attatcagat atgctgaatc aagaacgtat tcctcatgaa gtgctgaatg cgaaaaatca
    11821 ctttaaagaa gcagagatca tcatgaatgc tggtcaaaaa ggtgctgtga cgattgctac
    11881 caacatggct ggtcggggaa cagatatcaa gttaggactt ggggtaagag aacttggtgg
    11941 tctagcagtt atcgggacag aacgacatga atctcgtcgt atcgataacc agcttcgagg
    12001 acgtgcagga cgtcaaggag accctggtat gtcacagttc tacttgtcat tggaagatga
    12061 tttgatgaaa cgattcggtt cagaacgaat caaagctttc ttagatcgga tgaagatcgg
    12121 tgaggaagat gctgttatcc aaagcaagat gctgacgaaa caagtagaat ctgcacaaaa
    12181 acgtgtagaa ggaaataact atgacacacg taaaaacgtc ttacaatacg atgatgtgat
    12241 gcgtgaacaa cgtgaagtaa tctataaaca gcgacaagaa gtaatcatgg aagaaaaaag
    12301 cttatccaag ccactaatga acatggtcaa acggacaatc agtcgtgtag tagatgcgca
    12361 tacacaatta gagaaagaaa attggaattt ggaaagtatc atcgattttg ccggcagtac
    12421 tcttgtccat gaagatagta ttactcttgc agatatcgaa ggaaaaacag ctcaggagat
    12481 caaagagtat ctaaacgaac gagcacaaga agtatttaat accaaagcgg ctcaactaaa
    12541 tggtccagag caactgttgg aatttgaaaa agtcgtgatc ttgcgtgttg tggactctaa
    12601 atggacagac catattgacg caatggatca attacgtcag tcaatcggct tacgtgctta
    12661 tggacagaac aatccattag tcgaatatca aacagaaggc tataagatgt ttgaagatat
    12721 ggtaggcgcc atcgagtatg aagtcactcg aatctttatg aaagctgaga tccgtcaaaa
    12781 cgtacagcgt gaacaggttt ctcaaggaac agcttctcac tcagaagacg gagatgccaa
    12841 agaaaatagt aatactagcg cgaaaaaaca gccaatccat gtgaacaagg tcggaagaaa
    12901 tgatccttgt ccttgtggaa gcgggaaaaa atataagaac tgtcatggaa aaaatgcaaa
    12961 ctaatttctg acagcaggat tggtgagaca aattcttttt ttaagaattc gtctcacctt
    13021 ctttctgtta tttgctgatt ctgttagact agatataatg aaaaaagtat agtgaaagaa
    13081 ggaaaaaaca tggaaatatc cgaaattcga aatctcctga cagaaatgca ggaaaaggtc
    13141 acaagtttca ggaggtctct ttgacttaga tcagctggaa gaagacatcg cggaagcaga
    13201 agcaaaaatg gctgaacctg gtttttggga tgacagcgaa gccgcacaaa aagtgatcag
    13261 tgaaaataac gctaataaag aaacctatga ccaattccat tctcttgccg aagaactaga
    13321 agaaatcgaa ttgatggtgg agatgctgca agaagaacct gatgcagcaa tgcaagaaga
    13381 agcagaaaca cgtatcaatg cattagatga aaaaatgaag acgtatgaat tatctatgct
    13441 gttagacgga ccttatgatc gaaacaatgc gatcgtcgaa ttgcatccag gggctggcgg
    13501 aacggagtcc caagactggg gcagcatgct tttacgtatg tatacacgtt gggcagagca
    13561 gcatggctat caagtagaaa cactagatta tcaagcaggt gatgaagcag ggatcaagag
    13621 tgtgaccctg ctgattaaag gacataacgc ttttggttat ctaaaatcag aaaaaggtgt
    13681 tcatcgcctt gtacggattt cgccttttga ctcagcgaaa cgacgtcata cttctttttg
    13741 ttcggtagat gtgatgccag agcttgatga aacgatcgat atagaaatca atccagatga
    13801 tttgaaaatc gatacgtacc gagcaagtgg tgcaggtggt cagcacatca ataaaacaga
    13861 atctgctgta cggatcactc acttgcctac aggaacagta gtagctagtc aggcacaacg
    13921 ctcacaattg aaaaaccgtg agcaagcgat ggggatgtta aaagctaagc tttatcaatt
    13981 agaagtggaa aagaaagaac aagaagcagc tgcacttcga ggagaacaaa aagaaatcgg
    14041 ctggggttca cagatccgtt cttatgtttt ccatccctat tctatggtaa aagaccatcg
    14101 gacaaacgta gagacaggaa atgtccaagc agttatggac ggtgaccttg atttatttat
    14161 tgacggctat ttaaaaatgc atctacaata agaacccatt gttaaattga aataaaattg
    14221 taagattttg caagcatgtt gaaacattgc catgctataa tagctaaaga ctgaaaaaga
    14281 tatggagaga acgccatgat tgaaatgaag gatgtaatga agaagtactc aaatggtacg
    14341 accgctatcc gcaatctctc gattgtcatc gaacaaggag agtttgtcta tgtggttggt
    14401 ccgtctggag ccggtaaatc tacattcatc aaattgatgt atcgagaaga aaaagcttca
    14461 aaaggaactt tgaacgtcgc aggtcatgat ctaatagcaa tcaaaaaccg cgaagttcct
    14521 tatctacgca gagaaatcgg cgtggtgttc caagactata aattattacc aagaaaaaca
    14581 gtatatgaaa atgttgcgta cgctatgcaa gttatcggca gaaaacctcg agatatcaaa
    14641 aaacgagtaa tggaagtgct ggaccttgtt ggtttgaaac acaaagttcg agtctttcct
    14701 agcgaattat ccggtgggga acagcaacgt gtctctattg ctcgggcaat cgtgaatacg
    14761 cctaaagtac ttatcgcgga tgaaccgaca ggaaatctgg atccagaaaa ctcatgggaa
    14821 atcatgaaat tattagatcg gatcaatgcc caaggaacta ctgtcgtgat ggctacacat
    14881 aacagtacga tcgtaaatac gattcggcac cgagtaattg ctatcgaaaa cggccgcatt
    14941 attcgagatc aagcggaagg ggaatatgga tacgatgatt agaacctttt tccgccattt
    15001 attagaaagt atcaagagtt tgaaacgtaa cggctggatg acagttgctt cagtcagtgc
    15061 ggtaacgatc acgttgactt tagtcggtgt attcatggca gttatcatga atgcaactaa
    15121 attagctcag gatatagaag gaaacgttga tgtatctgta ttcgtagata tcggtacaaa
    15181 agacgaagag atgaaaaagc tagaaaaaga attgaatgac ttagatcatg ttaaaaaagt
    15241 tgaattttca agtaaagaac aggaattgaa aaaaatccaa aacgaaatgg gtgattcctg
    15301 gaaattgttt gaaggtgaca ataacccgtt atatgatgtg tatgtagtaa gtgctactga
    15361 tccttcttat acaaaacaag tagcagaaga agcagcagat ttgaagaatg tttctcgttc
    15421 tgattacggt ggagcttcct cagatcggat tttccaaatc gctggtgcag ttcggacatg
    15481 gggattagtt gcagcggcat tgctgctgtt cgtagcgatg tttctcattt cgaacacgat
    15541 tcggatcacg attttgtcta gacagcgtga aattcaaatc atgcgtttag tcggtgcaaa
    15601 aaatggatat attcgctggc cgtttttcct tgaaggtggc tggatcggtc tgatcggtgc
    15661 gattcttccg attttgatca tcacatttgg ttatgcatgg tttttccaat tgatcaatcc
    15721 atcattatta cgttcccatt attcattgat tcatccacaa aatatcgttt ggaaaatcaa
    15781 tcttttaatg gtcggcattg gtgtgatcat tggttcactt ggctcgatca tttctatgag
    15841 acgtttcttg aaattctaat accacaatat aaataaaatg agaaagttgg acagtttcgt
    15901 tccagctttc tttttttatt ggctaggaga atagaaggaa gcggcttttt tccctttact
    15961 ttaaaaaaca acttgggtat tcttaagttg gtagaggaag aggtgaagcc gtgaaaatta
    16021 tcgttgacaa tttttttacg atccttttga ttatgaatat tttgctttcc tttatcatcg
    16081 tttttcgaga aagaaaggaa actgctcaaa catgggcatg gctgctcgtt ttgatgttca
    16141 ttccagttgt aggatttatt ttatatattt ttttaggaag agggatttcc aaagagaaaa
    16201 tctttgattt gaagatccaa gggaaaatcg gtaagaacct ggaaattgaa gaagacaaac
    16261 aggcattgat gcgagggctt tatcctcatc cgcctactgg acaagtagat gtgaaacaat
    16321 tgatctatat gctcacggta tttgaaagta cgctctatac gactaaaaat gaaatcactt
    16381 tatttacaga tggtcgtgaa aaattcgatg cgctgattga agatatcaag caagcagaag
    16441 accatatcca cttccagtat tatatttatc gcagtgatgc attaggtgaa gaagttcgag
    16501 atgcattgac agaggcggca agacgagggg taaaagtcag agtccttctt gatgcatggg
    16561 gttccacaca agtttcttcc agcttttttc agaatttgaa aaaagcaggc ggagaaatcg
    16621 catttttctt tcctctattt gttccgtata ttaatcctcg gatcaattat cgcaatcatc
    16681 gtaaaatcgt agttattgat gggaaaatcg gatatacagg tgggttcaat gttggaaatg
    16741 aatatctagg attggtaaag aaatttggtt attggcgaga caatcatttg cgtatttacg
    16801 gagaagcggt gtatatcttg caaaaccgtt ttttgatgga ctggaattct cagcatacaa
    16861 aagaagtagg ctattctccc aaattttttc cgtccattca ctctactggt gatatagctg
    16921 ttcaaatcgt gacaagcgga ccagataccg aacatgaaca aatcaaaatg acttatttga
    16981 aaatgatttc gttagccaaa cgggagatat tgatccaaac gccttattac ataccggatg
    17041 gatcgattca tgaagcactg aagttggctc ttctttcagg cgtaaaagtt catatccaaa
    17101 tacctaacaa accagatcat cttctcgttt actgggcaac gtattctttc gctgcagagc
    17161 tgatcgagta tggagcaagg atcgaaacat atgagaatgg atttattcat gcaaagacga
    17221 tgattatcga tggtggcatt gtttcagttg gctcagcaaa tatcgatgtt cgttcatttc
    17281 gcttggactt tgaggtcaat acactgattt atgatgaacg aatggcttca cgtgtaagaa
    17341 aagcattttt tgaagactcg aaaatttcta cgcatctcac aaaagaaatg tatgaaaacc
    17401 gaggaataat catcaagatg aaagaaggat tagcaagatt gatttcgccg ctcctttaaa
    17461 acacaaaaag gacatgagaa ccatcaacag atggagatct cgtgtccttt tctaacgggg
    17521 ccatttctga gagtacgtca gaaatggaga aagcgtctcg catgagcgaa attaactttg
    17581 gggtatctct tgttttttgg ggaaagcaag aaataagcta atatatgcta ttaaaagaga
    17641 cacatttcaa agccagtata gcatagaaac ttttaatgag atagtttttt ttacaattat
    17701 gaggatttat tttgttattt aatgattttg attcgttttc atcaagtaat gaaaatgata
    17761 aaataaacat actttaggat taaaggagat ttctaatgaa aaaacttaaa ttattcttac
    17821 caataatagg attagctatt cttttatcag gctgttcaaa gtggattgat cgaggagaat
    17881 ccattacggc tgttggctct tctgctttac agcctttagt agaaacagtt gctgaaactt
    17941 accagacaga aaatcctgga aaattcatca atgtacaagg gggcggaagc ggaactggtt
    18001 tgtctcaagt acagtcagga gcagtcgaca tcggaaactc tgatttgttt gcggaagaaa
    18061 aatcggggat cgatgcctca gcattagtcg atcatcgagt agcagttgtt gggatcactc
    18121 ctattgtgaa caaggatatt ggcgtaaaag acatcagtat ggaaaacctg aaaaaactat
    18181 ttacaggcga gatcaaaaac tggagtgaag tcggtggaaa agatcagcca gtagttatat
    18241 tgaaccgtgc ttcaggaagc ggaacccgta gcacgtttga aaaatgggta ttggacggag
    18301 aaacagctgt tcaggcacaa gaacaagatt caagcggaat ggtgcgccag atcgttgccg
    18361 acacacccgg tgcaatcagc tatgttgctt tttcttatgt ttccgatgat gtagatacgt
    18421 tgaatgttaa cggtgtagaa ccgacagatg aaaatgtgac aacgaacaaa tggacgattt
    18481 gggcatatga gcatatgtac acaagagatc atccaaaaaa actgacaaaa gaatttttag
    18541 actatatctt atctgatgaa gtacaagata acatcgttgg agaactaggt tatatccctg
    18601 tttctcatat gaaagtcgaa cgtgattggc aaggaaatat cgttaaagag taatctttac
    18661 acaaaattta caaacttagc taaactttta catgttatta aaaccaaatt gatgttcaat
    18721 ttgtacactg agtgagtatg ttaaacctga ttaaggggga gtcatctgtg gaagatgtga
    18781 aaaaagtatt attaacaaaa tcaaaacgtt caaaaatgga acagcgtgga aaattcatca
    18841 gttttttatg tatcgcgctg atcgtgttgg ttgtattatc tattttttat tttgtagcta
    18901 gtaaaggatt ggccaccttt tttactgata agatcaatgt atttgatttt ctgtttggta
    18961 cagattggaa tccaagccaa atcggagcaa atgggaaacc aatggtaggg gcattgccta
    19021 tgatcacagg ttcgtttatt gtgacctttt tatctgctat tgttgccaca ccatttgcca
    19081 ttggtgcagc agtattcatg acggagatct ctcctaaaaa cggaacgaag atcttacagc
    19141 cggtcatcga gctgcttgta ggtattcctt ctgtcgttta tggttttatc ggtttatcag
    19201 ttatcgtgcc ttttgtcaga agtatttttg gcgggacagg gtttggtatc ttagctggta
    19261 catttgtgtt gttcgttatg atcttgccga ctgtcacaac gatgactgtt gatgcattaa
    19321 aggcagtacc acggcactat cgggaagctt cattagcgct tggtgccaca agatggcaaa
    19381 caatctacaa agtcgtgcta cgagcagccg tgcccgggat tttaacagca gtcgtttttg
    19441 gtatggctcg tgcgtttggt gaagccttag ctatccaaat ggtcatcggt aatgcagcat
    19501 tgatgccaac aggacttact actcctgctt ctacattgac gtccatctta acaatgggaa
    19561 tcggaaatac aatcatggga acagttgaaa ataatgtgtt atggtcatta gcgctgatct
    19621 tgctattgat gtcattattc tttaatatcg tgatccgtat gattgggaag aaaggagagc
    19681 tgaaataatg aacgcaaaac gctcagataa aattgctaca ggtgttttat atgtagtttc
    19741 ggggatcatc gtattgattt tagcagcttt actactttat atcttagttc ggggaatacc
    19801 gcacatttca tggtccttct tgaccgaacc ttccaaagcc taccaagaag gcggtggaat
    19861 cgggatccag ctatttaact cgatttattt gcttttgatc acgatgatca tcagttttcc
    19921 tatttcttta ggtgcgggta tctatctttc agaatatgct ggaaaaaact ggctgacaga
    19981 tgtgattcgc acgtcgattg aaatcttgag ctcattgcct tctgtcgttg tcggtttatt
    20041 cggtttcttg gttttcgtta ttcaattcca gtatggtttc tcgattattt ctggtgcatt
    20101 ggctttgact gtattcaact tgcctctttt gactagaaca gttgaggagt ctttacaagc
    20161 ggtccattac acacaacgag aagcaggatt ggctttaggt ttgtcacgct gggaaacagt
    20221 gataaaagtg gtggtttcag aagcgacacc agggattttg actggtgtga tcctaagttc
    20281 tggaagaatt tttggtgaag cagctgcatt gatctatacg gctggacaaa gtgcacctgc
    20341 gttagatttt acgaactgga atccattaag tgtttcaagc ccgatcagca ttttccgtca
    20401 agctgaaaca ttagctgtcc atatttggaa gatcaataca gaaggcacga tgcctgatgg
    20461 tgtttctgtt tcggctggcg cttctgccgt gttgatcatt gctgttttac tgttcaactt
    20521 cggcgctcgt gcgattggca aacgattata ccgaaaaatg acttcagcct aaggagaaga
    20581 agcaaaatga aaaaatataa ctggaatgaa acaaatgtca tcaccatgcc aaaagagaaa
    20641 gaagtagcac tatatacgga tgatctacat gtctggtatg gcgacaatga agcaatcaaa
    20701 ggagtggatc tggaatttga aaaaaataag atcactgcac tgattggtcc ttccggctgc
    20761 ggaaaatcta cgtatcttcg ttcgttgaat cgaatgaatg atgggatcgc taatacgaaa
    20821 atcactggga aaatcatgta tcaaggcgta gatattaatg cgcctcaagt ggatgtttat
    20881 gagatgcgca aacggattgg catggttttt caacgtccta atccgttcag caagtcgatt
    20941 tatgacaata ttacatttgc tttgaaacag cacggcgaaa aaaacaaaaa gaaattagat
    21001 gaaatcgttg aaacaagctt gaaacaagct gccttgtggg accaagtaaa agacagcttg
    21061 gacaaaagtg ccttggcttt atctggcggg caacagcaac gtttatgtat tgctcgtgcg
    21121 atcgcgatga aacctgatat cttgctgcta gacgagccgg caagtgcttt ggaccctatt
    21181 tcgacaggaa cggttgaaga aacacttgta aacttgaaag aagactatac gatcatcatc
    21241 gtgacgcata acatgcaaca agcggctcgt atcagcgact atactgcctt tttctatcta
    21301 ggaaaagtaa tggaatatga caaaactaac aagatattta ccagaccaaa aattcaggca
    21361 accgaagatt atgtgtccgg tcattttggt taagacggag gcctcaacat gacaaaagaa
    21421 attatttcat ctaaagatct tcatctgtat tatggaaaga aagaagcact aaaagggatc
    21481 gatctaagtt tcaatcaagg ggaaatcact gcgatgatcg gtccttctgg ttgcgggaaa
    21541 tccacttatc tgcgttcttt gaaccggatg aatgacctga ttccgaatgt aacgatcacc
    21601 gggagtgttt tatataaaga caaagacatc tatggtccaa aaacagatgt tgttgaacta
    21661 agaaaagaaa tcggcatggt attccagcag ccaaacccat ttcctttttc tatctatgaa
    21721 aatgtcatct atggattgaa attaaaaggg gaaaaaaata aaaaagtcct tgatcaagta
    21781 gttgaagaaa gcctgaaggc agcgtctgtt tgggatgatg tgaaagacaa attgcataaa
    21841 agtgctttat ctttatccgg tgggcagcag cagcgtgtat gtatcgcacg tgttttggca
    21901 gttaatccgg aaatcatctt attggatgaa ccaacaagtg cgcttgatcc tgtttctact
    21961 gggaaaatcg aaagtatgct gttagaatta agagagcggt atacgatgat catcgtgacc
    22021 cataatatgt ctcaagcttc acgtatttca gataagacgg cattcttttt agatggtcac
    22081 ttgatcgaat acaatgatac gaagaagata ttcatgaatc cagaaaaaca agagacagaa
    22141 gattatattt caggtcgatt tggataagga ggaaacggaa tgctacgtac acaatttgaa
    22201 gaagaattgc tgaatctcca taatcagttc tacgaaatgg ggatgatggt gagcagcgcc
    22261 gtgcacaagt cagttcgtgc atacatcaaa cacgataaaa tcttagccca agaagttatc
    22321 gaaaacgata taaatatcaa taacatggaa acacgcttgg agaagaaaag ttttgaaatg
    22381 atcgcattgc aacagccagt tacaactgat ttgcgtatga tcattacggt catgaaagct
    22441 agctcggatt tagaacgtat ggccgatcat gcggtgtctg ttgctaaatc cacgattcgc
    22501 gtaaaagggc agacgagaat accggaaatc gaaaaagaga tctccgatat gtccgattat
    22561 gtgaaaaaaa tggtggacaa cgtcctagtt gcttatgtca aaacagacga aaaagacgca
    22621 cgtacgatcg ccaatatgga tcaaagagtc aatgactatt tcaatcgaat ctataatgca
    22681 acaatcaaaa atatgcagga aaatcctgag acagtcatca gtggaacaga ctacttgaat
    22741 gttgccagtt atctagaacg gatcggagac tatgtgacaa acatctgtga atggatcgtc
    22801 tacttggcta cagggaaaat cactgagtta aatacgaacc acaatgagat tttctaaact
    22861 aaagcggtga ttctgccgtt tgtttaggaa aagaggttgt gaaatcagcg gttgtgaaaa
    22921 agctgttctg catcaagtaa taagaacagt caaacaaaaa tagctcctca tatttttcat
    22981 ttggctggac ttattaccgc agatgcaagc ttttgaacac tgtttattcg ggaaagggtt
    23041 tgtgacaaaa tatgtcacaa acccttttca tcatttcata gaaaggataa agaatgcaaa
    23101 aaaaaattag catcatcact atatttttta tttgtgtgct tacttcatgg gttattcagc
    23161 atcaagctgc tttatatgaa gtacctgtca tcgagatcga aaaagtacga ataaaagagg
    23221 atcatcaatt gattacagga agactgatga acacgaatca aaaaggagaa tcgatttatt
    23281 tatcggttcc ttatcaaaaa aatcaaatag aaacagcaaa tataaaagcg agacaaatgt
    23341 atctagtcca aatgcatgga gatgaatggc agctagtagc aaaaaaaaat gatggctggc
    23401 ttttcttctt cacaagtatc ttcattggcg ccatgttgct tattggcgga aaacatggtc
    23461 tatttgcttt aggaagtctc atcatcaata tcgttttact tttacttttg ttgctcatct
    23521 ttactaaaat agaagggatt tctctattaa atatatctat cctgttcgtc gttataggga
    23581 tgattattag catattggct ttagatggat ggaatagaag cagtataatc aaaatttcag
    23641 ctgcagttac gagtgttttt ttagcatttt ttatttgtct tttgacaatg aatctttgga
    23701 aagaccaagg actgcgctat gaggaactga attatctgac acgaccgtat cgttctgttt
    23761 ttctttctag cgtactgatt ggaacagctg gaggaacact cgataccgtt atcacagtca
    23821 ttgcgacatt agaagaattg actaagcgac aaccagaaat aacagcgaaa cagttatggc
    23881 tttcgggaag gcaagtgggt cgagatgtga ttggctcgat gaccaatatc cttttgttcg
    23941 tctatatcag tgggtcagtt ccatcgcttc ttttatattt aggaaatcaa tggtcgttca
    24001 aagaaacgat agaacagcat ttatcattgg aatttttacg cgtcattgct ggcagcttag
    24061 gaatagtatt atcgatccct atctcggtcc tcttttttct gactgtaagg aggctgaaac
    24121 gatgaatgta cttattatac ttgttgggat tttagcaatc agcgtgctac ttattggcgg
    24181 tacgcaaggt atgtatattc ttttaggctt attcatcaat ttagggatct ttttcttgtt
    24241 gttattcggt tatcatcaga agtggccgat actggcattg tcggtcatag gatttttatt
    24301 gattgctgtg gttatcttat tttttatcaa cggttataat ctaaaaatgc gtgcagcttt
    24361 tgtgagtata ctgatctttt tattttgttt cctactattg cttcctatca ctgatttttt
    24421 agcaatccaa ggttttacaa cgatagaact agaagaatta tctggcttag ataagacttt
    24481 agcgattgat tttagattac ttacgagatc gttgcttctg attagtctta gtggtgctgt
    24541 tttagatgcg agtgtagcaa tttctagtgg gacattcgaa gtatatcagg caaatccgca
    24601 attatctttt taccaattgc gccattctag tttcgctatt gctaaaaaaa tattggcgtc
    24661 aacagtaatg acacttcttt ttgcattcat gggaagttct ttagcgttga tcctctggtt
    24721 tattgattta gacattcctt ttcagcaagt gatcaatgaa aaatcatttg ttctagaata
    24781 tacgatggct attcttacca cattgtccgc actacttgtt cttcctttaa cctgcgtgac
    24841 tacagcatat ctctttacaa aaaagaaaga acaactcaag atggagtaaa agttacaact
    24901 taagtcctat gacattttta ctgaatcagt gcataatatg gatgtaagga aaagcttgca
    24961 aacaattttg cttaaggagg caatttccat gaaagaaaga gaacgtattt tagatttagt
    25021 gaaaaaagga atcctttcga ctgaagaagg cttagattta ttagaaagca tggcaactga
    25081 aaaagacgaa aagcaaatca aaaaagaagc agacaaggta actgctagtc ataaagaaaa
    25141 agatcaggca agtcaattga tcgataaact tgaaaacggc gaagaagaaa tttctgagcc
    25201 agttgatcca aaggaaagag aaagacaaga tcaagaaaac ttagaaaaga tcttagatga
    25261 actagcaact gaagcgaata aaacttcggc gcgtctagat gaagtaaatg ctcaattcgc
    25321 tgacaataag gcagcacgca atgagaaaca ggaagcattg atgcagctga atacaaaaga
    25381 agaattaggc gagttgacag aagaagaatt agcacagcgt cagacgttag aagcagaaat
    25441 caaagagtta gaagctagtg gcgatacact gttagaagaa caattgaaat tagaagcaga
    25501 attaaaagat atccgtaaga atcaatggtc tgagaaaaaa gaaacgttta ctgataagtt
    25561 tgaattgcca gatgattgga aagatcaagc aacagataca ctgaatcaag taggcgaaaa
    25621 aatgtcagaa gctggatcac agttaggaaa attcttgaag aaaacattcc aaactgtttc
    25681 agaaacagtg aatgacaaca tggaatggaa agatgtcagc ttgcgtgttc ctgggattgc
    25741 gacaacgaaa tttgagcatg aattttacta tgaagctcct gcagcaagta tccttgatat
    25801 caaggcagca aacggaaacg tgacgttaaa aacgtgggac agcaacgatg tgaaagttga
    25861 agctaagatc aaactttacg gaaaaatggg tgctgaacca tttgaagcat tctctgaacg
    25921 gagccagatt gaggttaatg aagatcatat ctccttccaa attccaaata aacgtgtgcg
    25981 tgcagacctt gttttctact tacctaaacg agtatacgac cacgcagcta ttaaacttct
    26041 aaatggcaac atcatgattg aaacattgga agctaaagat atttatacaa aatctacgaa
    26101 tggtaacatc atcgtggacc aattaactgc aacgatgctg gaagtagaag gtgtaaacgg
    26161 caatatcgat attcgaaacg ggaacatctt agattctatt atcgaaacag taaatggtac
    26221 agtgacattt ggtgcgacag cagaaaacct ttctgtgtct ttggtaaatg gtgatgtacg
    26281 tcttacgatt aaagaagata atttgaaaaa agtagaagca agttcagtga acggaaatgt
    26341 caaagtcgct ttaccagatg gcatcggttt ggaaggacat gctaaaacaa gtcttggaag
    26401 tatcaacagc cgtctgtcca attacgaagt agttcgtgag aaaaaagaac ggacaaacca
    26461 aatgttgcaa ttccgtcgtg tttcagatgg tgaaatcgcg caagtgcaac tttcaacgac
    26521 aacaggaagt atctatttaa aagatacaga taaataaaga ggttgtgaaa gagatgttca
    26581 gccttgagca ataagaagcg attttgtaaa atagcttccc ctattttaca aaattgtgcc
    26641 ttattgccga taggctatct cttgaacacc gtttaccaag agattatgaa cttgccaatt
    26701 atcgccgagt cacaaggaat aatttcaaaa aaagctcgtc acgttttatg aatatggtaa
    26761 tttgtgaccg agaattttgc aaaaagacac cgattaagaa gaaggttgtc acaacgattt
    26821 tcttcattta aaatagtaga acagaggtga gacatgatga aaaaaaagtt gacaaaatcc
    26881 aataaaaatg ttgttttgac agggacttta gcaggaatag gagaatacat cgggattgat
    26941 ccgactgtta tccgtgtgct ttatgtattc ctgagcttag ttgcttttgg aagtccaatc
    27001 attttataca ttttattagc tttgattatc ccatctgcgc gttctgcacg aacaaactat
    27061 ggacataaca atgcatatta tgaaaaaaac aattatcgtg aacaaactcg tgaacaaaca
    27121 aaaaaacgca aagaagcgga aaaaatcgat gatgacgaat ggagtgactt ttaatgtcgt
    27181 actttcaacg tattttagtt aatacgttaa cattcatatc attggctgtt ctttttcctg
    27241 cgatggtcca tgtgagcagt atttggacgg ccgttcttgc cagcttcgta ttgtctgtgt
    27301 tgaatatgtt tgtcaaaccg attttgacga tactgtccct gccctttaca ttagtcacat
    27361 tgggattgtt tacgtttgtt atcaatgcag cgattcttaa aatgacctcc ttttttgtgg
    27421 gagcagaaca ttttagtttt tcttcattcg gtgcagcgtt gttagtagct atcttgatgt
    27481 cgcttgtcaa tgtaattgtc agcgaacata acagtagaaa ataaaagaaa gctgtgccga
    27541 actatcggcg cagttttttt atttagaatc aggaatgcgt tttctattac attggtaatt
    27601 aaataaaaac gtttttttta attggtagta cttttggcta gacaatgata aaatgataga
    27661 gaacactcta atagaaatga ggtagcatga atgtcagaag ttgtaaaagt tagccaatta
    27721 gtgaaaaaat tatctttgga aatcgtgaca ggcgacgaga aaagcttaga ccgtgtcatc
    27781 gtaactgggg acatctcacg cccaggacta gagttgactg gttattttaa ttattattcc
    27841 cataatcgtt tacagctatt tggcagtaaa gaaatcagtt ttgcggaacg gatgatccca
    27901 gaagagcgtt tgatcatcat gagaagatta tgtagtgaag atatgcccgc atttattatt
    27961 tcaagaggat taccagctcc agaagaaatg atccaggcag caaatgaaaa tgggatagct
    28021 gtactgcgtt ctcccatctc aacttctcgc ttattaggag aattatccag ttatttagat
    28081 ggtcgccttg cccctcgaac aagtgtccac ggtgttttag tggatgttta cggattaggt
    28141 gtactgattc aaggtgacag tgggatagga aaaagtgaaa cagcgttgga gttgatcaaa
    28201 cgcggtcatc gattgatcgc agacgatcga gtagatgttt atcagcaaga tgaattaacg
    28261 gtgatcggag aaccgcctaa aatcctggaa catctgattg aaatccgagg aatcgggatc
    28321 attgatgtaa tgaatctttt tggtgcgagt gcggtacgtg gctctatgca agtccagttg
    28381 gctgtttatc tggaagcatg ggcaaaagat aagaaatacg accgtctggg ctctgaagac
    28441 acgaccgttg aaatcgcgga agtaggcatt ccacaaataa aaatccctgt caaaacagga
    28501 cgaaacgttg caatcatcat tgaagtagcc gcgatgaatt tccgtgcacg tacaatgggc
    28561 tttgacgcaa cgaaaacatt tgaagaaaga ttaagtcgct tgattgaaga aaactcaggc
    28621 aacgactagc aaggagtgac aatatgttag cacaaatcaa tcgagttgct tttgagcttg
    28681 tagggattcc tatctactgg tatgcattga tcatcgtatc aggtatcatt atcgccatgt
    28741 ggcttagcag cagagaggct gttcgggtag gattaaaagc agatgatgtg acagatttta
    28801 tgttgtgggg attgcctatc tccattattg gcgctcgctt atattatgtt ttatttgatc
    28861 ttccccagta tatagctgat ccaatccaaa tattcaatat ccgttcagga ggcttagcta
    28921 tttatggcgg actgattgca ggcgcgatca cgctgtattt ttttactaaa tatcatttta
    28981 tttctttgtg gactttttta gatatcgctg caccaagtgt attattagca caagcaatcg
    29041 gtcgttgggg aaatttcatg aaccacgaag catatggacc agacacgacg cgaagcttct
    29101 tagaaagtct gcatttaccg caattcatca ttgataatat gtatatcgaa gggatctatc
    29161 gtcaaccaac atttctttat gaatcacttt ggagtttgat tggatttatt ttgcttttgt
    29221 ttttacgaaa gaaaccacac cttttgaaaa aaggtgaagt ccttttatgt tatctaatgt
    29281 ggtatagttt cgggcgtttc tttattgaag gattacgtac agacagtctt tactttttag
    29341 gtgtgatccg tatttctcaa ttattatctg ctgtgttatt tgtcggtaca ttgattctat
    29401 ttatttggcg cagaaaaaaa ggaaatttac cggattatga ccgctcatta ggaaaaaatc
    29461 aacaaattat ctgattttta gagcgttaaa aatgaaaaaa atttcaggag tggtaaaatg
    29521 aattatagag tagcggtttt aggaccaggc tcttggggta ctgctttggc acaagtcctc
    29581 gccgaaaacg gacatgacgt tcggatttgg ggacatcgtg cttcacaagt agaagaaatc
    29641 aacacccaac acacgaatca acgttattta ccagatcatt gtctacccga ttccatccaa
    29701 gcattcacca atatggaaga tgcagtaaaa gacgcagatg cggtgctatt cgtcattcca
    29761 acgaaagcaa tacgttctgt tgcaaaagaa ctagtgccta aattgaatac aaagccagtg
    29821 atcatccatg caagtaaagg attagaacag gggacacata aaaggatctc tgatatcttg
    29881 ttagaggaaa tcccagtaga aaaaagaaaa gctgttgttg ttctttctgg accaagccat
    29941 gcagaagaag tagcgaaaca tgacatcact acgatcacag ctgcaagtac agatgcaaaa
    30001 gcagcaaaat atgtacaaga gctgttcatg aataattatt ttcggatcta taccaatcct
    30061 gatgtgatcg gtgtagagat gggggcagct ttgaaaaata ttattgctat tggtgctggc
    30121 gctattcacg gcttagggtt tggcgataat gcaaaggcag caatcatgac gcgcggatta
    30181 gctgagatca gccgtttagg tgtagcaatg ggtgccaacc cgctgacctt tatcggatta
    30241 agcggagtag gcgatctagt cgttacttgc acaagtgttc attctaggaa ctggcgcgca
    30301 ggcaacttgt taggacaagg gcagccccta gaagaggtgc tagccaatat gggaatggtc
    30361 gttgaaggtg tgaatactac ccaagcagcg atggaactag cccaaataat ggaaatcgaa
    30421 atgcccatca ctcaaacaat ctatgaagtg ctgtatgaac aaaaagaaat caaaacagcc
    30481 gcaaaagaaa tcatgttacg tgatgggaaa aaagaaaatg aattctcatt aacaattttt
    30541 tgaaggagcg aatgtatatg aaagtcaaaa aagcagttat tccagcagca ggattaggaa
    30601 caagattttt accagcaaca aaagctatgg caaaggaaat gttgccaatt gttgataaac
    30661 caacaatcca gttcatcgtt gaagaagcgt tgaaatcagg tatcgaagac atcttgatcg
    30721 ttacaggaaa agctaaacga ccaatcgaag atcactttga ttcaaatatt gaattagaaa
    30781 caaacctaag tgaaaaagga aagaccgaat tattgaaatt agtagaagaa acgacagatg
    30841 tcaacttgca tttcatccgt caatctcatc caaagggttt aggacatgcc gttcttcaag
    30901 caagagcctt tattggaaat gaaccatttg tcgttatgct aggcgatgat ataatggaag
    30961 acgaagtacc attgacacag caattaatcg atgattacga acgtactcat gcttctacga
    31021 tcgccgttat gcaagttcct catgatgaaa cttcaaaata cggaattatc aatccaggag
    31081 aagtattgga agatgggtta tacaatgtca aaaactttgt tgaaaaacca gatccaagca
    31141 aagcaccaag tgatttagct attattggac gttacttatt gactccagaa atttttgatg
    31201 tattgtccga gcaacaacca ggagccggaa atgaaatcca actaactgac gcaatcgata
    31261 cattgaataa aacacaacgc gtatttgccc gtaaattcac aggtaaacgt tacgatgtcg
    31321 gtgacaaatt tggttttatg aaaacaagta ttgaatatgg tctaacacat ccagaagtag
    31381 gtgcaccttt acgtgagtac atcctgtctt taggtaaaac attagaagca gaaggaagca
    31441 agatcgatca taatccaaaa ggaataacag aaatcaaaga agaaaagaaa aaataaccct
    31501 tttttctaac gagcttcagc tgctaattga ataaaaagta gttgaagctc gtttttattt
    31561 gtttaaaaaa cacgaaaatg ctatccttat agaaaagaga gacaagaagg gagtttgatc
    31621 atgtcaggag gagaaattgc aggattgatc gcagcaatcg cctttgcgat acttgtcgtt
    31681 tttatcgttc gtgtgttgtt gcaagtttct aaaactttag aaaaagtaga tcagactgta
    31741 gcagaagcaa atacgacgat cgaaatcgtc acaaaagacg tagatctttt gtctagacaa
    31801 gtagaaggct tactggtcaa aagtaatgaa ctgctcttgg atatcaatgg aaaagtcgca
    31861 acgatcgatc cattgttcac agcagttgct gatttaagtg aaagtgtatc agaacttaat
    31921 cattctagta aaaacatcgc gacgaaagtc ggcggaatcg gtaaaacgac agctaaagcg
    31981 acggttgccg gaaaagtcgg tgggacagcg atgaaatttt ttaaaaacaa aaaacatact
    32041 gaaacgacag gaggaaaata aaaatggcta aaaaaggtgg atttttatta ggtgcagtag
    32101 tcggaggcac agccgcagca atcgccgcgt tattgttagc accaaaatct ggtaaagaac
    32161 ttcgggatga attagctgcc caagcggatg atttgatgga tatggcttca gattacacag
    32221 atatggcgaa agaaaaatcg actgagttaa caggtattgc aaaagaaaaa gcgcaagatc
    32281 tttctcaaca agcaggtgat ctagcaggta gtgtcaaaca aaaagtaagt gaatcagcag
    32341 atgaagcagg aagtgaatct gacgatatct tgttaaatct taaagaacaa tcagctgact
    32401 tatctaatcg tttcaaaaaa acggcagaag atatgaaaaa acctgcagat gaattaggaa
    32461 gtattgctaa agatacttca gaagatatca tgattgatgt aaaagacgct gctggtaaag
    32521 caggagatac tgtcaatgaa ggaatgtcag aagcaaaacc agttgcagat gactcaaaag
    32581 aaatgatgaa aatggcaaaa gaagatgcga aagaagctaa aaaagaaata aaagatacaa
    32641 tgacagattc atctacaaat cactaaaatc acaaatttat agaataaaaa ggtgggagcg
    32701 gactagctct cacctttttt actcttattt ttctaaaaca agtaatt
//
LOCUS       DACDIH010000025        32680 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDIH010000025 DACDIH010000000
VERSION     DACDIH010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.025"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            135..1160
                     /locus_tag="IV281_001269"
     CDS             135..1160
                     /locus_tag="IV281_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8780293.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(1755..5408)
                     /gene="rpoC"
                     /locus_tag="IV281_001270"
     CDS             complement(1755..5408)
                     /gene="rpoC"
                     /locus_tag="IV281_001270"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP8780294.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(5487..9104)
                     /gene="rpoB"
                     /locus_tag="IV281_001271"
     CDS             complement(5487..9104)
                     /gene="rpoB"
                     /locus_tag="IV281_001271"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8780295.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            9832..10800
                     /locus_tag="IV281_001272"
     CDS             9832..10800
                     /locus_tag="IV281_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8780296.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            10818..11723
                     /locus_tag="IV281_001273"
     CDS             10818..11723
                     /locus_tag="IV281_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8780297.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            11720..12526
                     /locus_tag="IV281_001274"
     CDS             11720..12526
                     /locus_tag="IV281_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8780298.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            12683..13651
                     /locus_tag="IV281_001275"
     CDS             12683..13651
                     /locus_tag="IV281_001275"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP8780299.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(13968..14492)
                     /locus_tag="IV281_001276"
     CDS             complement(13968..14492)
                     /locus_tag="IV281_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP8780300.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            14779..16590
                     /gene="scm"
                     /locus_tag="IV281_001277"
     CDS             14779..16590
                     /gene="scm"
                     /locus_tag="IV281_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP8780301.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(16745..17731)
                     /locus_tag="IV281_001278"
     CDS             complement(16745..17731)
                     /locus_tag="IV281_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8780302.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(17754..18320)
                     /locus_tag="IV281_001279"
     CDS             complement(17754..18320)
                     /locus_tag="IV281_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP8780303.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(18334..18750)
                     /locus_tag="IV281_001280"
     CDS             complement(18334..18750)
                     /locus_tag="IV281_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP8780304.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(19207..21141)
                     /locus_tag="IV281_001281"
     CDS             complement(19207..21141)
                     /locus_tag="IV281_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8780305.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            21443..22378
                     /locus_tag="IV281_001282"
     CDS             21443..22378
                     /locus_tag="IV281_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP8780306.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            22468..23571
                     /locus_tag="IV281_001283"
     CDS             22468..23571
                     /locus_tag="IV281_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP8780307.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            23584..24537
                     /gene="menA"
                     /locus_tag="IV281_001284"
     CDS             23584..24537
                     /gene="menA"
                     /locus_tag="IV281_001284"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP8780308.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(25472..26818)
                     /gene="gor"
                     /locus_tag="IV281_001285"
     CDS             complement(25472..26818)
                     /gene="gor"
                     /locus_tag="IV281_001285"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP8780309.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFCGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(26952..28193)
                     /locus_tag="IV281_001286"
     CDS             complement(26952..28193)
                     /locus_tag="IV281_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8780310.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(28211..29134)
                     /locus_tag="IV281_001287"
     CDS             complement(28211..29134)
                     /locus_tag="IV281_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8780311.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(29355..31847)
                     /locus_tag="IV281_001288"
     CDS             complement(29355..31847)
                     /locus_tag="IV281_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP8780312.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(31854..32321)
                     /locus_tag="IV281_001289"
     CDS             complement(31854..32321)
                     /locus_tag="IV281_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP8780313.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(32457..32530)
                     /locus_tag="IV281_001290"
     tRNA            complement(32457..32530)
                     /locus_tag="IV281_001290"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32494..32496),aa:Arg,seq:acg)
     gene            complement(32588..32661)
                     /locus_tag="IV281_001291"
     tRNA            complement(32588..32661)
                     /locus_tag="IV281_001291"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32625..32627),aa:Pro,seq:tgg)
BASE COUNT         9562 a         6919 c         6059 g        10140 t
ORIGIN      
        1 agcttcttaa aaaaatatgg tctctcatga tcatgcaagc tattcctaaa ttttcaaaat
       61 catagacaaa aaagaatgtc tattgtacta tggaattatt ctaaaacgct cccaaaaagg
      121 aggtgcatat aaaattgaaa cctactattt atgacattgc gaaaaaagct aatgtctcaa
      181 aatcaactgt ctccagagta ttaaataacc agccaaatat ctctgataaa gcaaaagcaa
      241 gtgtacttca agcaatcaaa gaattgaatt atgaaccgag caaaattgca agaggactgt
      301 caggaaatgg ttttgacgcg attctagcat tatctcatcg tacttctcat acgacctatg
      361 gcaatccttt tttttcagat gttattcaat ccatttctgc cgtttctgaa caaaataact
      421 tcgacttgat tctacaaacg gcaaaaacac cagaagaaga gctagaaaaa tgcgcgaaaa
      481 agatccaaga aaaaatgatc cgaggaatta ttattttaag ctctacgatg gatgaaacac
      541 ttctttttga attggatcaa tacaatattc ctattgtggt aatcggaaaa gtcggaaacg
      601 actacacgaa catctactca gtggacacgg ataatttcca ggatagttac gatttggtgg
      661 aaacactgat caagaatgga caccgtcata tcggctgtct ccatgctccg acagaagttt
      721 atgtatccaa tgatcgtctc aatggttacc gtcaatgctt atttgatcac cacctagata
      781 tccgtaatga atggattata gacggcggct ttcgcttaga agatagcatg gcagcaatga
      841 atgatttaat gaagctacga gaaagaccaa ctgcgatttt ttctaccgat gcattaaaat
      901 cattgagtat ttacaaagtg gcagaacaat ctggaaagca gatacctgat gatctttcga
      961 tcgcaggatt cagtgatccc ttattgtcct ccctattttc gccacagttg actcggatag
     1021 acattccgac aaatcagtta ggaatcaaag ccacagaact tttattccaa ctaatccacc
     1081 accaataccc tgaagaaaaa agaatcattc tttcaaccga gattactctc acagattcga
     1141 tccaacaaca agaaatgtag aacagaatta taaaagaaca gaaacactga ggataagtca
     1201 gttactctgt tcttttttca atcactcatc cctcgttacc ccctcccggt attttagcga
     1261 atttttctta ttttccatct gttttagata gctgtggata actataatgt ttgtgttaaa
     1321 ctattttttc cacagggaag aaaaaactat ttttaaccac gttgtgtata agtttttata
     1381 gttcgttttt ttaaacacat ttacccttat ttcaatccat gttatttggg gatacttatc
     1441 tctaaaacat aaatagaata acagacatgt ctactggttt tgttttattc gaggaattat
     1501 tcaataatag ctagcaaaat attaaaaata accgctctat cttcccatca atttattttg
     1561 cattcaaaaa atgatctatc tttacttttg ttgattgact tctcttggaa gatgatcgtt
     1621 ccgatacctt aattggatac gaaccgatgt tgttaagatc ctttcccact tttcttctag
     1681 ttagacaaat tattttagac aaaccaaaaa agaggttgtg acaataattt gtcacaacct
     1741 cttttttaaa atatctattt tgtttgattg ttcattgcgt cttctgcagc catttgcgct
     1801 tcaatatctg aaatactgta gacattttcg cttgctacac cgacttcttt tggttccatg
     1861 tcacgataac gttgcatacc agtaccagct gggatgatct tacctataat aacattttct
     1921 ttcaaaccaa gaagaggatc tttcttaccg cggatcgcag catccgtcaa gacacgagtt
     1981 gtttcttgga atgatgcagc agataagaaa ctatttgttt ctaatgatgc ttttgtgata
     2041 cctagtaaga caggtcgaga agttgcagga acgcctcctg agactagcgt attgtagttt
     2101 tgatctttaa agtctgcgat gtctaataat gtacctggta agatatctgt atcacctgga
     2161 tccattacac gaactttacg cagcatttga cgaaccatta cttcgatatg cttgtcgccg
     2221 atttctaccc cttgcatacg gtaaacacgt tgtacttcac gaagcaagta gttttcaaca
     2281 gataatacat cgcgaacttg caacaattgt tttggatcga ttgatccttc tgtcaatggt
     2341 gcaccacggt ggatcatatc gccttctgct actttcatac gtgctgtata aggaacagta
     2401 tacgtacgtg tatctgtttt tcctttgatt gttacttctt tggtacgtgt tgcaggatct
     2461 tctgagatat cgatgacatc ccctgttact tctgtgatca ctgcttgccc tttcggatta
     2521 cgtgcttcaa agatttcttg gacacgagga agaccttgag tgatatcgtc tccggcaacc
     2581 ccacctgtat ggaatgtacg catcgtcaac tgagtaccag gttcaccgat tgattgagcg
     2641 gcgatcgtac caactgcttc tccgacttca acgtcagagc cagttgcaag gttgcgtccg
     2701 taacaatgtt tgcagacacc atgttttgta ttacatgtga agacagaacg gattgtcact
     2761 gtttcgacac ctgcatcaac gatttgtttt gcgatgtctt cggaaatcaa ttggtttcct
     2821 gcaatgatca attcatttgt ttcaggatga cgtacttctt tacgggtgta acgtcccact
     2881 aaacgatctt ctaatgattc gatgatttca tttccctcgc ggatggcttg gatttcaaga
     2941 cctcggtcag taccgcaatc ttcttcacgg atgattacat cttgggccac gtcaaccaga
     3001 cgacgagtca agtaacctga atcggcagtc ttcaaggctg tatcggtcat ccctttacga
     3061 gccccgtgag ttgagataaa catttccaag acggatagtc cttcacggaa gttcgaaatg
     3121 attggcaatt ccatgattcg accattagga gcggccatca aaccacgcat tccggcaagc
     3181 tgtgtaaagt tggaaatgtt accacgagct ccagaatcag acatcatgaa gattggattt
     3241 ttcgcttcca aaccttccat cagtttttgt tggatgctgt cttttgcagc attccaaaca
     3301 gcaatgacac gttcataacg ttcgtcatct gtgatcagac cacgacggaa ttgttttgta
     3361 atagtttcaa cttgtttatg ggcattttca atgatttctt gtttttcatt cagtacactg
     3421 atgtcagcga tccctactgt gataccggca tgtgtcgaat gtttgtaccc taagtctttc
     3481 atacggtcaa gcatcttaga agtttctgtt actttgaatc gtttgaagac ttctgcgatg
     3541 atgtttccta agtttttctt cttgaacggt aatactaatt cttgctcttt gatatgtgca
     3601 ggaatatcag taccagcttc aacaaagtat ttgtccggtg tttggacggt caagttgaag
     3661 tctgttggtt cattcagata agggaattca ggaggcatga tctcgttgaa gatgatctta
     3721 ccaactgttg tgatcatcgt acgttctttt tgccattcag taaatggttt ttcacctaat
     3781 gagttagggt tcacaccgat acgtgaatgt aaatgaacat atccgttgcg ccatgcgata
     3841 acggcttcgt tttgatctct gaagaccatt ccttcgcctt cgcgtccttc ttcttccatt
     3901 gtcaagtagt agttacctag aaccatatct tgggaaggag taacaactgg tttaccatct
     3961 ttagggttca agatgttttg agcagctaac atcaacatac gtgcttctgc ttgtgcttct
     4021 tcattcaatg gaacgtggac agccatttgg tctccatcga aatcggcatt ataagcttca
     4081 catacaagtg gatgcaaacg gattgcgcgt ccttgtacta aaactggttc gaatgcttgg
     4141 ataccaagtc ggtgaagcgt aggtgcccgg ttcagcaata ctgggtgttc cttgattacg
     4201 tcttctaaaa catcccatac ttcgtcttct tgacgttcga ttttacgttt cgcatttttg
     4261 atgtttgaag ctagttcgcg ttgaacaagc tcacgcatca cgaatggttt gaataattca
     4321 atggccattt cttttggaag tccacattgg tacatcttca agaaaggtcc aacaacgata
     4381 acggaacgac ctgagtagtc gacacgttta cctagcaagt tttgacggaa acgaccttgt
     4441 ttccctttca acatatgaga aagagatttc aatgggcggt tacctggtcc agtaactgga
     4501 cgtccgcgac gaccgttatc gatcaatgca tctactgctt cttgcagcat acgtttttca
     4561 ttttgaacga tgattccagg tgcattcaag tctaacaaac gtttcaaacg gttattacgg
     4621 ttgattacac gacggtaaag atcattcaag tcacttgttg caaaacgtcc accttctaat
     4681 tgaaccattg ggcgtaaatc tggtggaata acagggataa cgtccataac catccagctt
     4741 ggttgatttc ctgaagcgcg gaatgcttct aaaatatcca aacggcggat tgcacgtgta
     4801 cgtttttgtc cttgtgcaga tttcaattct tctttcaatt cggctgcttc tgcatctaga
     4861 tcaacattat caagtaattg tttaatcgct tctgcaccca tagcagcttg gaattcttgt
     4921 ccatattgtt cacgtttttc gcgatattcg cgttctgtca acaattgttt tttctctaaa
     4981 ttcgtatttc ctggttcgat gactacatat gaagcaaaat aaatgatttc ttccaaagcg
     5041 cgtgggctca tgtctaatac aagacccata cgtgaaggaa tccctttaaa gtaccagata
     5101 tgtgaaacag gagctgctaa ttcaatatgt cccatgcgtt cacggcgaac tttcgaacga
     5161 gtaacttcca ctccgcatcg atcacaaacg attcctttat aacggatacg tttgtatttt
     5221 ccgcaggcac attcccagtc ttttgtagga ccaaaaatcc gctcacagaa caacccttcg
     5281 cgttcaggtt ttaaggtacg atagttaatc gtctcaggtt tttttacttc accgtaagac
     5341 cagcttctga ttttttcggg agaagccaaa cctatttgca tactttcgaa tttatttaca
     5401 tcgatcaaaa ggggttccct ccatttcatt taatggcaaa ccgcaagacg gctgatcttc
     5461 gaccgccttg cagctcatag accggattag tctaaattat cttctgcggt ttcaaagttt
     5521 tggatgacat cgtctttttc atcttccact tgttcagcag ctttcttttc taattctttg
     5581 gcagtttgct gttcggcaaa ttttgtcaaa gcatcaacag tgatcaaatc gtcgtcttca
     5641 tcgtccatgt cgcgaagttc gatctctgtt tcttcgatat ccaagacacg catgtccaga
     5701 cctagtgatt gcaattcttt caccaatacg cggaatgatt ccggtacacc tggttttgga
     5761 attggttcac ccttgacgat tgcttcgtat gttttcacac gtccgactac gtcatctgat
     5821 ttgtatgtca agatttcttg taaagtataa gccgcaccgt aagcttccag tgcccatact
     5881 tccatttcac caaaacgttg tccaccaaat tgagctttac ctcccaatgg ttgttgtgta
     5941 accaatgagt aaggtccgat tgaacgtgca tgcaatttgt catcgaccat gtgagcaagt
     6001 ttgatcatgt acatcacacc gacagacaca cgtccatcaa atggttcacc tgtacgtccg
     6061 tcgtacagaa cagttttcgc atcgctggcc ataccggctt cacgaactgt ttcccatacg
     6121 tcttcatcac ttgcaccatc aaatactggc gttgctacgt ggatacctaa ctgacgtgca
     6181 gccataccca agtgtaattc caatacttgc ccgatgttca tacgtgaagg cacccctaaa
     6241 ggattcagca tgatatcgat tggtgttcca tcaggtagga atggcatatc ttcttccggc
     6301 ataatacggg atacaacccc tttatttccg tgacgaccgg ccattttatc cccttcatgg
     6361 attttacgtt tttgaacgat ataaacacga actaacatgt ttacgcctgg tgacaattca
     6421 tctccagctt cacgagtaaa gattttcaca tcgtgaacga taccgccgcc gccatgtggt
     6481 acacgtaatg atgtatcgcg gacttcacga gctttttcgc caaagatcgc atgcaataga
     6541 cgttcttctg cagataattc tgttacccct tttggcgtga ctttccctac aagcaaatcg
     6601 ccatcttgga cttctgcacc aatacggatg atacccattt cgtctaaatc ttttaatgcg
     6661 tcttccccga cattagggat ttcgcgtgtg atttcttcag gtcctaattt tgtatcgcgg
     6721 gcttctgatt catattcttc aatatggata gaagtgtata cgtcatcttt taccaaacga
     6781 cggctcatga tgatcgcatc ttcatagttg tacccttccc aagtcatgaa accaactaaa
     6841 acgttttggc ctagagccat ttcgccttgt tccatagatg gtccgtctgc cagagtatcc
     6901 cctttttcga ctttctcacc caaatgaacg attggacgtt ggttgtagct tgttccggag
     6961 tttgaacgac ggaattttgt tacatcgtat ttatctaatg caccgttgtc gcggcgtacg
     7021 cggatttcag aagcatcaac gtattcaacg acaccgtcat gtttacataa caaagcagct
     7081 cctgagtcat gcgctgattt gtattccata ccagtaccaa cccatggtgc ttgaggattg
     7141 atcaacggca ctgcttgacg ctgcatgttg gcacccatca aggcacggtt ggagtcatcg
     7201 ttttccaaga aaggaataca tgctgtcgca acggcaacta cttgtttagg tgatacgtcc
     7261 atgtagtcta ctttatcaat agatacttct aagttttcac tttgagcacg agccataaca
     7321 acgtcttgag caaatgttcc atcttcattc aatgggctgt ttgcttgggc tacgatgtaa
     7381 tggtcttcga tatcagctgt caagtagtcg atttggtcag taacacgacc tgtttcacgg
     7441 tctacacgac gataaggtgt ttcgataaat ccgaatttat ttactttcgc ataactggac
     7501 aaactattga tcaacccgat gtttgggcct tcaggagttt cgatcggaca catacggcca
     7561 tagtgggagt agtgaacgtc acgaacttca taaccggcgc ggtcacgagt caatccgcca
     7621 ggtcctaagg ctgaaagacg acgtttgtgt gtcaattcgc caagagggtt tgtttgatcc
     7681 atgaactgtg acaactgaga agaaccaaag aattctttga tacttgccac aactggacgg
     7741 atattgatca attgttgagg tgtcaatgtt tcagtatctt ggatcgacat tctttcacga
     7801 accacacgtt ccatacgtgc tagcccgata cggaattggt tttgtaaaag ttcaccgact
     7861 gaacggatac gacgatttcc taagtggtcg atatcatcga cattgccgat accttccatc
     7921 aagttcaaga aatagctcat tgaagcaatg atgtcggctg gacgaaccgt ttttacagct
     7981 gcttctggat agccgtttcc gatcacgttt acttcacgtt cagggtctct tggtgaaaat
     8041 actttgatga cttggacagt cattggatct gtgaccacac cgtcttctga tggatagtaa
     8101 gtcactgaat ttaggccatt atcaatgaaa ggagctagtg tttccatgac ttggtgagtc
     8161 agaactgttc ctttttcaac gatgatctcg cctgtttctg gatcaactaa tgtttcagct
     8221 aatgtcagat tcaatagacg tgttttcaaa tctaattttt tgtttacttt gtaacgacca
     8281 acgtttgcta agtcgtaacg ttttggatcg aagaaacgag cattcagcaa gttacgtgag
     8341 ctgtctgctg tttttggttc gcctggacgc aaacgttcgt aaacgtcttt caaaccttct
     8401 tctgttcttg agtcgcttgc gtttttatgc aagtcttttt cgactgtatt gcgtaatgtt
     8461 tcgctgtcgc cgaagatttc aaagatcgta tcatcggaac caaatcctaa cgcacgaact
     8521 aatacggtta aaggaatctt acgtgtacgg tcgatacgta cataagagat gtcttttgca
     8581 tcagtttcca tttctagcca agctccacgg ttagggataa cagtagaacc gaatccttct
     8641 tttccgtttt tgtcgacttt tccatggaaa tagacacctg gtgaacggac taactgagaa
     8701 acgattactc gctctgcccc gttgatgatg aatgttcctt gttctgtcat taatgggaaa
     8761 tcaccgaaga atacttcttg tgctttgatt tcgccagtct cgcggtttgt caaacgcaat
     8821 gttacatgta atggtgcaga gtagtttgca tcatgcgcac gagcttctgc tactgtatac
     8881 tttggttctt tcaattcata atctacaaat tccaaagata gattgccatt gaagtcatcg
     8941 attggcaaaa tgtcttcaaa catttctcta agtccctcat ctaagaacca ttggtaagag
     9001 tctgtttgaa tttcgatcaa atttggtaat tccaacactt cactgattct tgcgaaactt
     9061 ctacgttcgc gatgttttcc gtattttact acgtgtccag ccaagctctt cacccctcta
     9121 aaatgtcatt caaaaagact acccgatcaa ttgttacaat aatatttcaa atattatttt
     9181 taggtaacaa cagacatttt ttgcggcttt taggcaaaaa aaaaccaaaa atagaacatt
     9241 ctcttgaaac cttacttcgt gagcttctac ttttgtttcc ttatattaaa gccgaaacgg
     9301 acaatatttc gtttctgcct tttcttcatc agatagtttt tgcaacttca tatactacac
     9361 ttattttcta taaaagtcaa cacttcctgg cgtttcttct gctttttcgc aacattttct
     9421 taatgttttc aattttatgt tttcaattat ctgacaattc ttaattgaca gaccttttta
     9481 tctcggttat actttagaaa attcattttg tggaggaact tctatgcgaa ccaacatcaa
     9541 ccaaatcgca ccaacacaac tgaataactt ttactttagc tactttagat aggtttgtat
     9601 tcatcatgat ggatacaaac gttaaacgac agtttgtatc tatgatggct aaagaacttt
     9661 gtattgcagc aaaaagccac gtagacaaaa tgctaagtgg ctgagcagtg aaaatacaaa
     9721 gtaaaagtat caagcattct actttttcaa tttattttcc gacttttcaa tccatttgga
     9781 cattttgtct gaatggattt tttgtttccc aaaattaagg aggaaaaaga aatgaaaaaa
     9841 aatataatca tcggattagg cgctgccctg ctactagctt taggaggttg cgcacaaaaa
     9901 gacaattccg cagaggcatc agatggacaa aaaacaattg gcgtattgca acttgtacaa
     9961 cataactcgt tagatgctgc ctataaagga ttcaaagaag gattagcaga agaagggtat
    10021 aaagaaggcg ataatctaac aatcgattat caaaatgccc aaaacaatca agacaacctg
    10081 aaaagtatga gtgagaaact cgtcaaagaa gatcctgatt tgctacttgg aatcgccact
    10141 ccagcagcac aatctttatt aaatgaaaca accactatcc cgatgactgt cacagcggtc
    10201 acagacttgg aacaagccca tttagtcaca tccaatgaaa aacctggcgg caatgtgact
    10261 ggcacgacag atatggtgcc aatcgaaaaa caaatcggct tactgttaaa tatcgtgcca
    10321 gacgccaaaa caatcggcat catgtataac aacggtgaag caaactctaa gatccaagct
    10381 gatttggcag aaaaagaatt gaaaaaagcg ggcgtcaaag tcaaagtact gacagcaaat
    10441 tccacaaatg atgtccaaca agtgacgaaa agtctagcta aagatgtaga tggaatctat
    10501 attcctactg ataacacgtt cgcaagtgcg gcaagtgttg tcggtgaagt cgcaaaagaa
    10561 acaaaaacac ctgtcgttgc tggcgcagtt gatcaagtca aagacggcgg tctagcaaca
    10621 gtaggaatcg actacgaaag cttgggtaaa caaacaggga tcatggcagc aaaaatctta
    10681 gacggcaaga atccaagtga acttcctgtc gaaacagcga atgatttgga actgtatgtc
    10741 aataaagaca tggccaaagc attaggcatc aatccagaca gcatcaaaga agctgaataa
    10801 aacacaggag gattctcatg acaaatttgc tctctctttt cttatcatct gcatcgcaag
    10861 gggtcttatg ggccctactt gcgattggtg tatacgtcac tttccgtatc ttggacattg
    10921 ccgacttgac tgctgaagga agctttcctt taggtgcagg gatcgcggca gtaagtatta
    10981 caaatggcta tcatccattg acagcttgtc ttctaggctt tttagggggt gcggcagcag
    11041 ggcttgtttc cggactgctt cacacaaaat tgaaaattcc cgcccttctt gcagggatca
    11101 tcaccatgac tggtttatat tctgtcacat cccgtgtgat gggtgcgccg aacatctctt
    11161 tattgggtca gccaacgatc tttcattggg tcgagtcatt cggcataaca aaagacaacg
    11221 ctgtactgat cgtcggtctg ctttttgctt tgatcgttgt atgtttactt gttttgttct
    11281 tacgtacaga aacaggactt gctattcgtg caactggcga taatctggca atgagcgaag
    11341 caaacgggat caacacagat aatatgaaga tcatcggtta tatgatttct aatggatgca
    11401 ttgctctttc tggctccttg cttgcgcaaa acaacggctt tgctgattta aattctggta
    11461 ttggaacgat cgtaattgga cttgcttcta ttatcatcgc agaagttttg ttacgtaacc
    11521 agccactatt tctccgtctc ttgactgtcg ttttaggtgc ggtcatttat cgttttattt
    11581 tagcgttagt cttcgaactg aatgttgaac caagtgattc gaaacttgct tctgctttcg
    11641 tgctagtgat ctgtctttct ttcccgcaga tccagcaaaa attccattta cctcaaaaaa
    11701 agaaaggcgg tagtgtcaaa tgaatgacgt cttaacattg gaaaatctcc atcatacctt
    11761 tgaaaaagga acgatcaacg aaaatcatgt cctgaaaggc attaatcttt ccttagatcc
    11821 cggcgacttc gtgacgatca tcggtggaaa tggggctggt aaatctactt tgctgaacag
    11881 catcgccggt acattttctg tcgatcaggg tcaaatttta ttgaatggaa aaaatatcac
    11941 gaaaaaatct gttgtcgaac gttcgaaatc catcagtcgc gtattccaag atcctaaact
    12001 agggacagcc gttcgtttaa cggtagaaga aaatcttgct cttgctatga aacgaggaaa
    12061 aaaacgcggc ttcttccgtg gtgtcaaacc acaagaccga acatttttca aagaccagct
    12121 ggttcggcta aatttggggt tggaaaatcg tttatcctca gaaatcggtt tgctgtctgg
    12181 aggacaacgt caagctatta cgttattgat ggccacatta cagcgtccag aactgatctt
    12241 attagatgaa cacactgcag cgcttgaccc tgctacttcc aaaacggtga tggaattgac
    12301 agaaaagctg atcactgagc agaatctcac tgcttttatg gtcactcatg acatggaaga
    12361 cgcgatccgc tatgggagtc gactgatcat gctgcatcaa ggaaggatcg tcgtagatat
    12421 ccagcaagag gaaaaacagc agctcacagt gccccaatta ttagcgttgt tcaaacaaaa
    12481 cagcggcacg gcattaaaag acgatcaagt acttttatct tcataatcag aaagaggatg
    12541 ggacaaaaaa tgaacgccca ccctcttctt ttttctcttt ccttctttcg tttttgcttt
    12601 atgtatcaac cgttgtttct tcgaaaacta ttttgttctc tccttcttcc tgatagaatg
    12661 gatgggaaag gagcttgatg ccatgacaac gaaagacaag gtacttcaat tattgaaaaa
    12721 gacaccagat tttctttccg gagaaaagtt ggcacagcaa ctggaagttt cccggacaag
    12781 tatttggaaa gccatcaaag aattggaaaa ggcaggctat cgttttgagc acgggcctac
    12841 agggtatcgt tacctgccct ctgacgtact agatgcaaaa gaaatccagc aagagctacg
    12901 gtcgtttatt cctgatttga aagtacaaac gatgttgact tctgaatcta cgatgaaaga
    12961 tgccaaactg gcagcagctc aaggagattc tgtttcgaag ttatttgttg ctgatacaca
    13021 agaacaagct cgtggacgtt ttggacggcc ttttttcgct gaacctggac gaggaatcta
    13081 catgagtctc ttgctccatc cagacaaacg gtttgatgaa ttgcctcaat atacgatcat
    13141 cgcagccagt gcctgtgtga aagccattga atctgtaacc ggtcgttctg cttcgatcaa
    13201 atgggtcaat gatatctaca tggatggaaa aaaagtctgt gggattttat ctgaagcagt
    13261 cagtgatatg gaaagcggca ggatcagtca tatcatcatt gggatcgggc ttaatttttc
    13321 tgttcctcaa aatcattttc cagctgatct ccaagaaaaa gccatctctg tctttcccga
    13381 cggacaagct agtgtcacaa ggaatcagct aatcagcaaa atctggcaag aattgttcac
    13441 ttatctttct cacccagaaa aagcgttgga cttctataaa gaaaaatcat tcgtattggg
    13501 aaaaaccgtg tcgttcaatc aaaaaggaac gacttatgtc ggtcttgcaa aagaaatcgg
    13561 tgatcatggc gaactgatcg tccaaacaaa tgaacagaag atgagtttgt cttctggcga
    13621 aatcagtctc acatctatcc gtacgcaata attatcagat ttcacaaaag gacacctgat
    13681 ctcatacagt gtcctttttt ctgaattctc ttcaaaagac tctccttatt ctatcagctg
    13741 acagtttctc tatcctctag aaaaaggtat tctaaaaata tctataaagc atggtacact
    13801 ttctttaaat gaatcaatgc aaaaaacaag agagtcatca gtttacacga tcaaaagaga
    13861 tagcaggccg cttccttttt caatcttata gatagaccat gggtaacgat aaaaacaaaa
    13921 aagaacttgc gacttatttt gccgtaagtt cttttttagc tagtgcctta atctcttaat
    13981 cccaacaaac gtctcgcata gatcacctga ccagagtgca tagcagcatc atcaataata
    14041 gaaacgagac gcattcctcg tgtgactggt ggtgtccagt tctcgtcaat tacttcatct
    14101 agctcggaag gttccagcct tcttaagtaa gaaatagcaa aatcagcagc tcctcgcaag
    14161 tatcctagca gatcttctat ttgattagct tggatctgct ttgcttgttc cagactatgc
    14221 ttccaatctt gttcgttatc tgctatcgtc atcggaaacc ggctttccca gtcttgacta
    14281 tgccaaattg gagaagttcc tgctaaatca gcaatctgga aatctaattc tcgagcggta
    14341 tgccaagcca gccaagtcat tgatttgatc tgcggggctc tatcttccac tggaaaacga
    14401 ttggcctcct caatagagac gccttcaaac aaacgttcaa accgatcgta tgcccgatcc
    14461 acactctcgc atagcatatc taaataatcc atttattttc ctccttttta tttatcctac
    14521 ttactttttc ctaattctag gatacctact tatatgttac tacacaccct tattttttac
    14581 actttaacag tcaccatcat caatagtttg gagattgaaa gaaaaagata gtgtcgaact
    14641 atcaattgaa caagaagcaa caatatgaac gtttcattaa tattctttaa ataaacaatg
    14701 atttttcaaa cgaacagttt aagactagga gaaaaagagt ataataggaa aatgtttgaa
    14761 aggaaaggaa ggggaaaaat gaaaaaaagg gcaagtttac tagtcctagt tgctttgttg
    14821 ttcaatatct tttcgtatgg tgtggccgtt tcagcggaaa catacattcc taaagaccct
    14881 gggaatattt ataatgatct aactggtaac tatggcttgt taggaattgc tagtcaattc
    14941 cacgtatttg ctaaaaataa gacaactgtg aatgcacata cagacggaaa tatagcaaca
    15001 catgaacttg atgcaaaaaa taattttggt acagagcttc atgaaggttt attgaaccaa
    15061 gaaataaatt atatacaaaa aactgattct ttcgctaatt cttcaggcat acctacaggt
    15121 agcacaagaa tgaataaatt tgtcgttggc agtaacgtgg atgtagcact tcaagatggt
    15181 catcctacag tcaatggatc caggcttgat cacttaactc cagatgattt ttataaagat
    15241 cgtgctgggc atgaatatat tgatttcgag caggaatttg ctaaattgaa tgtagcagca
    15301 aatgatcttg caacgatcac tccagctaaa acatatactg ctgcagattt tccagacatg
    15361 aacaatcgga caatcgattt aactgattta tcagataccg ggtttttatt ggtaaatatc
    15421 gatgcagaag tgttaacgat gaatacgcca cttcaaatca ttaatccaaa tgatcaagta
    15481 gttgtattta atgtcatcaa cagttcttct gcattgaacg tacagtcacc aatcaaatac
    15541 aatgatcgtt caaatcatga aacagaagat ttttcggatg cgaatatctc ttggaatttt
    15601 ggaaatgaga tgactgattt gacaatcagt gctccttttc aaggaacaat ccttgcgcct
    15661 aatgctacta tccgcattac tcaaaaccaa gacggaacga ttataggaac aaatgtgatt
    15721 ttagatgcag ctaccaatcg ttgggatcct aacgaaatat ttatcagcga caatggtaca
    15781 gatacgacag acacaagcga cagtacagat acatctacca caagtgatag ctcagattca
    15841 tcaacttcaa gcgacagtac agatactacc tctaccacaa gtgatagcac ggatacatca
    15901 gcttcaagcg acagtacaga tactacctct accacaagtg atagcacgga cacatcagct
    15961 tcaagtgaca gtacagatac tacctctacc acaagtgaca gcacggacac gtcagcttca
    16021 agcgacagta cagatactac ctctaccaca agtgatagca cggacacatc agcttcaagt
    16081 gacagtacag atactacctc taccacaagt gatagcacgg acacgtcagc ttcaagcgac
    16141 agtacggata catctaccac aacaagtgat agcacggaca cgtcagcttc aagtgacagt
    16201 acagatacta cctctaccac aagtgatagc tcagattcat ctaccactac aagtgatagc
    16261 acggacacgt cagcttcaag tgacagtaca gatacatcct ctaccacaag tgatagctca
    16321 gattcatcaa cttcaagcga tagtacagac acgtcgtcta ccacaagcga tacacgtcat
    16381 gcaagtgtct ccttctcaaa aacaagttcg gataacagga cgaactttaa cttaactaat
    16441 aaaacaagca atgacaatgg aaacaaatca aaaagagcac ttcctaaaac tggaagtcaa
    16501 tcgaataatt ggataacact cgcaggcgtt atattacttg taatcggttt aagaatcact
    16561 tttatgagtt ataagaaatc gaaaaggtaa aaaaatattc aagataaaaa agagcaacgt
    16621 cgctaacgga cgttgctctt tatttttgct ttttatcata aatacaagaa ctacaattca
    16681 atttgtcaaa aaacacgtag atttccttct gagaaattta tttacgtatc gaacctttcc
    16741 ctattcagtc tttgcgatcc aaaatcagtt gggtaaggga aaagagattc tcttttgttc
    16801 gaagagaggt ggcaggtaat ttctttattt ccttcaaagc cttttctgtg taagaggccg
    16861 ctaacttcct agcttcttcc acgcctcctg tatgttcaac gattttgcgt acggcgtcga
    16921 catcttcttc tgtcattgcc gctttcttct ctaaataagg tagtagcttt ggtttgtttt
    16981 tttgcaaaga gtagatcaac ggtaaagaat agaccccttg acgcatgtct tctagtacag
    17041 gtttgccgat ttcctggctc tcttttgtat aatctaaaat atcatcgata atctgaaagg
    17101 ccatcccgat agaaagtccg atatctcctg ctttttttgc gaagcgctcg cttgttccac
    17161 tttcaaaagc gccaacagaa caacttaatt ggaacagttc agctgtctta cctgaaatat
    17221 tttccaaata ttcttttaca gaaagttcat aattatagcg atcatccatc tgccctaatt
    17281 cgccattcaa tactttttcc atactgcgag aattcaattg gagacttttc aaggaagtgg
    17341 agtaatcaga caatagttta aaacagcaga caaataaata gtctcctgca tagacagaaa
    17401 cgacattacc aaactgacta cggatattag gctgaccacg tcgcagatcc gcttcatcaa
    17461 cgatatcatc atggatcagt gtggccgtat ggagcatttc gatagaagct gccaaagcga
    17521 tggctttgtc tcgatcttgc tctggtccaa attgtgagaa caatagctga taggcaggac
    17581 gcaacagctt tccgccggca tggatcgttt ctttgattgc ttgttcgaca ggtttgtttt
    17641 tcaggcggac agtttcttcc atccgtttta gtgtttctgt caattcttta cttaaagcag
    17701 gatagttttc ccacattgga tgtagcttca taaaaaaact ccttgtgtga cgattatttt
    17761 ttggtaaaca ttgggctcca tttttgtttg gttccctgct cataatcttt ttggaatcgt
    17821 tgaagtgtct gcacatgtcg taaaagataa ttcgctgcga tcccgattgc aatccctgat
    17881 aaaatcccta aaactgaaag gactggcaag tacaacatga ctgtccatga ttgtgcaatg
    17941 aaagaagcca tcaaaagctg tccgacatta tgcatgaaac cgccggccgc actaattccg
    18001 atgatactga cacgttttgg tcctaattgc ttgaccaata acattccaaa ataactaagc
    18061 aaagcaccac tcatactata aaggaatgtg gaaatcgttc cgcctagcaa agtagtcagg
    18121 aatagtcgta gccaaagtag taagaagctg tctcgttttt tcatggtaaa aatggcaacg
    18181 atcgtgatta gattggctaa gcctagtttt gcaccaggcg caaaagaaaa aggataaggg
    18241 atcatatttt ccaataaacc aatgatcact ccttgtgcaa caagtagcga aatataaatc
    18301 attttttgta atttactcat ggtgtgtgaa ggtctaataa atcagactgt cttcgtcacc
    18361 tccatctgtt gaacggactt cgatcactaa tttatgtggc aaacagacga tggtttcgcc
    18421 atttttcgtt gcccagcctc gacgtacgca gatctggtca tcgcaatctg cttcttttat
    18481 ccgtactcga tcgccatcta tctcgatcaa gttataatct ccgtgttcat ctttgtaagt
    18541 atatgtgttg ctctcttctc cagccatcaa agggaatgtt ttgatttctg tgccatccac
    18601 acgcagtacc gcttcttttt ctgctgttgc ggtgttttgc tgaagaccaa aaatcatgac
    18661 tggtaaaaaa gaaagaacag tcaaaagcaa aataataaaa acatcccaag gtttgagcag
    18721 acttttttca gcaaattctt taaggctcat ttttgttctc ctttttccag attaactcac
    18781 ccaattttag cataaaaaca agaggttgtg acacctgtgt cttgctttga gtaataagaa
    18841 cgaagaatag aaaacagctt tccatgtttt ctaattcttt gggcttatta ccgagaagca
    18901 gcaggtggaa caccgtttat aacaagaggt tgtgacagaa gtgggcaact tcaagaaata
    18961 agaaggaatt tacgaaaatt acttttcaag tttttgtgaa atttcggctt atttttcgaa
    19021 gaagctactt ctggaacacc gtttataaca agaggttgta acagaggttg tgacacctgt
    19081 gtcttgctca tggaggctta tctcgggcat cttattcctt taattttaca taaaaaggag
    19141 gctgtgacgg tttgtctcag cccctttcta ttgaaatctt gcattcaaat aacgttcttt
    19201 atctatttat ttcttgccgt actgggaact tggtctaccg taccaccaat tacctagtgt
    19261 acgttgtagc catggaatta cccagcgatc caacccaatc gcacgtccgg aaccgttcat
    19321 caatgcgaaa gcaacaaaaa tgaaccaaat gtttacccag taaaacatgc ctgataagca
    19381 gaacgtgatc accaatacaa tcgttgtcgc actgcatagc catgtgaata atccagcaat
    19441 caatgctaat gccaaggcaa tttcaaagaa taccatgaat ttttggaaga acattgctac
    19501 atcattgttt ggcatgatca cttgcatgat ctttgtgaac cagtctggtg acttctcaat
    19561 cacagccatt ggttcttcac cgtatgcata actcaaacca aaatgtgcag cttgagtcgc
    19621 tgcttctccg gcacttgccc ctgctccgct ggcagctgtg gttgcatcac cagcagcaga
    19681 agcaccactg gcaacatctg ttacattgta ttcccatgtc caagggaaca cactgtttcc
    19741 agtgaaccat gatccttcac caaaccaagt acttggtttg aacacttcac cattcccaat
    19801 gatttttttg gaagcttcaa cgaaccaaac taaaccatag aaaatacgaa gtggcacact
    19861 ccataagaca ttgccgtaac gagaagaatg tcctcgagtc acgttgcgat catctttgat
    19921 atggaaaaac tcatgcatta aatattggaa catataataa ccagaacgaa tatcaaagaa
    19981 gtatttcaaa ttcacgatat gtttcattac gatagctaaa aatccgctta aatggatctt
    20041 atcaaatagg ttagctaccc cccatttggc accaatagaa accataaatc cttgatagtt
    20101 tcctttaaat ttatgctttt ctccgccttt gatcgaagcc acgatattcg ctgcggctgt
    20161 gtgtcctgtt tgttcagcag cttgaacgat ctgcggtgtc ggtgttcctg ggaattcttc
    20221 ataaaagact aaatcaccaa tgatatagat gtttttatct tcgtagcctt ttgcctgcat
    20281 atattcattg gcgatcaacc gctgtccgcg tgctgattct aatccaaagt cagcagcgtc
    20341 agaagttcct ttgacaccag ctgtccaaat cagtgtgtga gtcggaatgt tgcgtccgtc
    20401 tttcaatttg atatgatcag cagccacttc tacgattggt gcattcaata ataattccac
    20461 gttgtgctta cgcaaatagc gttcagcttt tgcagcatca tttcgatcca acatattcaa
    20521 gatcgtcggc attgcctcta cgaccatcag tgtgatttcg tttggatcta atttataatc
    20581 tttggctagg cgatttttcc agtcgatcag ttccccaatc atttcaatac cggtaaatcc
    20641 agaaccacag acaacgaaag tcagcatggc tttacgcagt tcatcatctg gttccaatgc
    20701 agcttttgct actgtcttct caatatgttc ccgaatcctt acagaatctt caaatgacca
    20761 aagggtgaat ccgtgtgctt tgacaccagg tgtaccaaag tcattcggct ctccgcccat
    20821 tccaaggatc agctgatcaa aaggatagga acctaatttg gtcttgacga tttttcgttc
    20881 tttatcgata ccgattacag tgtctgtgac aagttgtaca ttcttttgac gagcaaacaa
    20941 atgctgcaga tcgtattgga ttgcttctgg ttctacacgt ccgccagcta cttcatgcaa
    21001 ctcggtcatc atagtatggt acgaatgacg atcgatcaga gtaattgcta catcttcttt
    21061 tttcagtttc tttgccaagt attttgtagc tgaaacaccg gcatatcctg ctcctacaac
    21121 gacaatattt tgctttgcca ttgatcatcc gctccttata gactatctat ataatgaata
    21181 tatgataatt cttttagttt gatacttatt acacaatctc catatattat atagatgttc
    21241 tgttcttttg tctaactcct tttggatttt tttcaaattt taaaataaag cgttttttca
    21301 cgaaatttgc tgaaagaaaa cgtttgaaat attacatggg ttatttatca aaaatagttg
    21361 acttataggc taaaaagatt agaatgtata atgaatcaca aagaaaaatt gtggactaga
    21421 aatggaatgg tggggtatta aaatgaagaa aaaacgtttt gtaactggat ttgccgtatt
    21481 agcattttca gctctagtgt taggcgcttg cggcgcagat gacaacggaa gctcaaactc
    21541 ttcttctgaa tcatctacag cacaatcttc gactgcaaaa agcacaacag aatcatctgc
    21601 aaaagttgta gctggcggag atttgcaaga cggcacatat aaattagaag aaaaaaatta
    21661 ttcaaatggt taccgtgcag tctttgaaat ggttgtaaaa gacggcaaaa tcactgaatc
    21721 aaaatatgat aacgtcaatg aaaacggcga atcaaaaaca aaagatgcag aatacaacaa
    21781 aaacatggaa gcaaaatctg gcacaaaccc agaaaaatac attcctgagt tgaatgacca
    21841 attcttaaaa gcacaaagcg caagcggtgt ggaagtcgta actggagcta ctcactcatc
    21901 tgaaagcttc caaaactacg cacaacaatt gatccaagct gctcaagctg gtaacacaga
    21961 tacaatcgaa atcgacaatg gtgctgattt gaaagacggc acttacaagc ttgaagaaaa
    22021 gaactactct aacggctacc gtgtgcaatt cgaaatgaca gttgctggcg gcaaagtaac
    22081 tgaatcaaac tttgattata tcgacaaaga cggtaaatca aaacaagatg atacagaata
    22141 taatgaaaat atgaaagcaa aaagcggtac agaaccaaaa acttacatcc ctactctgaa
    22201 cgacgaattc gtcaaagcaa tgggcgaaga agatggctca cctgctaatg tagaagttgt
    22261 tacaggtgct acacacagct cacattcatt catcatgtac gcacaacaat tagtgaatgc
    22321 agctgaaaaa ggcgacactc aaacaatcga agtagataat atcgtaacag aaaaataatc
    22381 acataatcat actaataaaa gttggagggt gggacgatag ccagattagg cagtcgtctc
    22441 acctttcaaa atgaagagag gaaaaagatg cggaaaaaac tactattttc cttaatcaca
    22501 ctgggcatct cttttatggt gctaactggc tgcagcaact cagcaaataa aacaaccact
    22561 gaaagttcag cagctaccaa gatattgaaa gatccatatt ctgatgaaca gttcttgtta
    22621 gggacttacg ttcgtatccg tgtgtatgat gaaggcaaag aatcagcatt gaaacctgcc
    22681 ttcgatcgag taaaagaact tggtgataaa atcacgatca atcaaaaagg ttctgagatc
    22741 gatgaagtga atgagcaagc tgggatcaaa ccagtaaaag tttctgatga cgtctacacg
    22801 ttagtcaagc gggcctacga atatagccaa gattctcaag gcggattcga tatggcgatt
    22861 ggtgcaatca cacaattatg gcgaatcggt tttgatgacg ccagaaaacc ttcccaagaa
    22921 gaaatcgatc aggcattgaa attagtggat tatcataaga tcgaactaaa tgacaaagaa
    22981 aaaactgttt atctaaaaga aaaaggaatg atcatcgatt taggtgcaat cgccaaaggt
    23041 tacattacgg acgaagtagt gaaagtcttg aaaaaacaag gagtgaccac tgccatcgtt
    23101 gacttgggcg gtaatgtcta tgtcttaggc cacagcccaa gaggcgaaaa ccaagactgg
    23161 acagtcggca tccaagatcc gaacatggct cgcggatctg ttttaggttc tattaaagaa
    23221 cggaacaaaa cattagttac ctctggaatt tacgagcgtt atttagaagt agatggcaaa
    23281 aaataccatc atttgtttga tccaaaaacg ggttatccgt tcgacaatga tatcgcaagt
    23341 gtcacgatca taacagataa atcaattgat ggtgacgggt tgtctacagc agtcttttca
    23401 atgggtgtga aaaaaggact ggaatacgtc gaaagcgaat tgaataacgg gacggaagcg
    23461 atcttcgtga caaaagacga taaagtatat gtgaccgatc caatcaaaga caccttcaaa
    23521 ttatcggagg actcccatta cacgatgggt gaccgttctg aactaaaata aggagtacga
    23581 accatgacac taaaaatctt tttagaagta gttgaaatac gaacaaaggt ggctagtgta
    23641 tttccttttg cggtaggggt attattctct atcgcttttt ttcatgagat acattggcta
    23701 aacacttttt tattttttat tggaatgcta gtttttgatt tagcgactac cgctatcaat
    23761 aattacatgg attatgaaaa ggctcattct gaagtatata agtatgaaga aaatgtcatt
    23821 ggccgcagaa atctatctcc tgtattgatt cgaaacatga ttttagctat gattgcattt
    23881 gtttttgtta tcggcgttat tctaacgatc aatacgggtt ggcttttact tttgatggga
    23941 tttatctgtt gttttattgg agtattctat acattcggac cgattccttt atctcgaatg
    24001 cctttagggg aaattttcag tggggtgact atgggccttg gaatttttgc catggtcgtt
    24061 tatgtcaata cattcggttc agatgtctat gatctgacag tcaacttttc atctggacaa
    24121 tttgctttgg taggaaatct ttgggctata gcagcaatcg tattagcttc cttacccttg
    24181 gtgtttacga ttgccaatat catgttggct aataatttgc gggatcttga ccgtgatatt
    24241 gaaaatcatc gctatacact agttttttat atcggacgtc ctgcgggaac agtcttattc
    24301 cagctattga tgtatgcttg ctatttcgtg attttaatgg ggttgttgag cggtatctat
    24361 gaatggccga tcttggtcac cttcttgacc ttgccaattg tgaagaagca tttggatcaa
    24421 ttcaagcaaa cgttacccca gcctatcagt ttcagctatt ccatcaaaaa tatggtctta
    24481 ttcaatgcca gttatgtatt ggggctatta ctaacaatca ttatgcaagc catttaacag
    24541 catgaagagg ttgtgaaaaa actgccctgc atcaagtaat aagaacagcc aaataaaaat
    24601 agctccttat atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
    24661 gtttatcagg aggttgtgaa aaagtcgctt agtttcgagt attaagaaaa attttgtgaa
    24721 aatggctttc cacatttttg cataaatttg gcttaatacc gagaaactgg cttttgaaca
    24781 ccgtttatca gaggttgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga
    24841 aaatggcttt ccacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaac
    24901 accgtttatc agaggttgta aaatcagtgt tttgtcctga gtattagaga aacaaatgga
    24961 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
    25021 actatttgtt cggaaaaagt gactaactat gacacaattt ttttcacagt cccttttttc
    25081 acttgatttg attcgtacca gcgaacttga cgagaacttt cttggtttgg taactatatg
    25141 gtattctttt aacatcctcg tttgcttctt ctaatttatt tatacaaagt cacacttttt
    25201 taaacatctg ttcttcgata catttctatt tcttttaaac atcgatacca atattttgaa
    25261 cttaagagtg cataccaaat ggattcattt actcttcgtc agttacgaag ataatcaaaa
    25321 tctccaactt ccctacttat ttcgttttga gaattatcga agcaatctta cataaataac
    25381 taaaaaacaa aaaaccagaa aatcagtata ctgattttct ggttttacat ggtttgttca
    25441 ataaaaagct gttggaactg ttaatattaa attatcgcat cgttacgaat tcttccgctc
    25501 ctgttggatg gatggctact gtattgtcaa agtcttcttt agttgcaccc atcttgatag
    25561 ccacggcaaa accttgcagc atctcatcga caccaacacc aatagcatgt aagccgacga
    25621 ttctttcttc ttttccgaca cagatcaatt tcatttcgca tttttggcga taatcattta
    25681 atgcaaagta cattggggta aagcgtgaac gataaacttt caattcattt tcgccatatt
    25741 tttccaatgc ttcttcttct gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga
    25801 ctgtcggaac aagtttatag tcaagataag aatctttctt tccattaaat aaacgttctg
    25861 ataaacgtct accagctgcg attgcaacag gagtcagatc taatttaccg atcacatcac
    25921 caacagcaaa aatatgttca tccgttgtat tttggtattt atctactttg ataaaccctt
    25981 ttttgtccaa ttccacattt gtattttcca gacctaattt ttcggtattc ggtacacgtc
    26041 caccggcaaa aagtacacaa tcaccagtta aggtttcacc attttcaaat agcaccgtat
    26101 attcgtcatt gtttttagta atttctttag caatatgatg tgcgtaagtt tttattccag
    26161 cttcttggta catttggatc aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc
    26221 gttcttttcg atatgcccac atgacttcac tacctaaacc acaaaataca cctgaaagtt
    26281 cagccgcaat gtatccagca cctaaaacga ttgctctctt aggtaactct gttaattcaa
    26341 aaaatccatt tgaatcaatt gcgtattctc caccaggaat ggtcatttta cttgcacggc
    26401 ctccagtagc gatcaagatt ttaggagcac gataggtttc accgtttact tcgatcgttt
    26461 gactatcgat aaaagtagcg taacctttta tagcctcgac atgattgcta tctaatcctc
    26521 tttgataagc accgtgtaga aaatcaatat atttttctcg tctttctact agaccggcga
    26581 aatcaacatt ttttaaatct gcttggatac cgtaacttga ggcatctcgt tcaattgttt
    26641 ccaaaatcgt acttgcttgc cacataactt ttttaggtac acaaccgacg ttgacacatg
    26701 ttccgcccaa ttcattcgct tcaatcagca aaacttttgc gccatgcatc cctgctctgt
    26761 ttgctgaagc aataccgcca gagccgccac caattacgat gtaatcatat atttgcattt
    26821 tgttcctcct tattgaagag atcaatcaaa acctttattt aacgatcctt tttctacaga
    26881 aaacaagtgt aacacttttc ttgaattttg tatgcgaaga tgcttaaaac agggatatat
    26941 cagctttttc cttatttacg ttttcggatc atatctccag gtttcaccgg ctgtgcgact
    27001 ggcatagtta aaatcatcat tggatttggt gctcggtcaa tggattcgcc ttcttcatta
    27061 tgcataactt caacggtttg actgaaatga ctgaatcctg gaccataaaa ttcgatttca
    27121 tctcctacag aaaacaagtt tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa
    27181 gataatactt caccgacaaa tttatatact gggattttac gacgttcacc gaataattgt
    27241 tcgttttcag ttggtgtatt gtagtaaaaa cctgtagcta attctcgttg tgctactttc
    27301 cataactcgt cgatccattc ttgcttgcag acatagtttt caggatcttc catataactg
    27361 tctacagccg ctttatagac atttgacacc gtagatacat aatggatcga tttcattctt
    27421 ccttcgatct tgaagctgtc tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt
    27481 gacatatcca ctgcactcat cgagaattct tcttccactt cgcctgtatc agttaaactg
    27541 tttcgttctt gaccaaaagg catatcgaac aatccatact tccagcgaca agattgagaa
    27601 catccgccac ggtttgcatc ccgcatcgac atatggttgg acaatgtgca tcgaccagaa
    27661 taagagatac acatggcacc atggataaat gcttcgattt cggtatccgt atttttacga
    27721 atctctgcca cttcctccat tgaaacttca cgagctaaaa cgacacgttc caacccttct
    27781 tctttccaga attccaatgt ctcaaaatta gttgctgaag cttgagtgga taaatggatc
    27841 ggcagacctg gggcttctgt cgcacaaatt tcgatcaaag ctggatcaga aacaatcact
    27901 gcagaaatac cgacatcacg caaactgcgg aaaaattccc ctgctccatc ttcatttcct
    27961 tcatgggtga ccatattagc agcaacgtat accttcgcat catgttcttt tgcaaaagca
    28021 actccttcag ccatttcttc ataagtaaaa ttacctgcac ggcttcttaa accatatgca
    28081 ttcccgccaa tatatactgc atctgctcca taatggatcg cggtttttaa cttttccagt
    28141 gttcctgctg gcgctaggat ttctggtcgt ttcaattttc tttttactgt catttctttc
    28201 atcatactcc ttatttgata gcagaaggat caatataaaa gaatcctgta tctaattcac
    28261 ggttttcagg atgcaattta ctgatttcgc tagtcatttt ttccgctttt tcttgatccc
    28321 agctaccttc taaaatttgt ttttttgctt cggcgaatag tcgagtgatg tttacgaatt
    28381 tttcgcctgg agtaaataat ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt
    28441 cattcaattc attcgagaga ttcagatcat tgctggcaaa aatatgtgtg ccatgactat
    28501 cttcataaat agaataatgt gtctcttctt ttttaggttc cgagataaaa agacctcttt
    28561 cacgatcttt ttgttcgtct tgtttcgtat agttatagta attctgtagc agtggacgtt
    28621 tggattgatg gatgcatgta gcaccataca caagggtttc tacaggaata tcgagttttt
    28681 cttccatcgc cttcatctct tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc
    28741 cttttttcgc ccagaaattg atttgacgtg cgctcgttac taatgtttca gcatcataaa
    28801 tatacggtat acgcaattca ggattttttt tcatcgtata aataatacct gtatcaccaa
    28861 ctgtgatcac atctacgttt atttctttta aaaaagccaa atattcaggt acatttttca
    28921 ttttttcagg atgaatgatc ccatttacag caatattcgc ttttttacca tacttgtgtg
    28981 ctaattctac caattgtcgc tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa
    29041 attgctcttc accgaaatat agtgtatctg cacctgcagc aagcaactgt tctgcttgtt
    29101 caatagtttc tactgtggtt atgagttcga tcataatttt ctcctcctac aacttcataa
    29161 tagtagcata gctctaaaaa ctttccaata cttcttctat ttaaagtcgg atttttgaaa
    29221 attatttcac aaaaaatcaa aataaagttc gatatattct gtccaaatat aaaaagagat
    29281 tgtgacaaaa atcttgtcac aacctcttaa tcaagtctta ctattataaa aattcagcta
    29341 cacaggccaa gtaactatag ggaacctact gtttcctttt taggtccgcg aacattcaat
    29401 gtgatcttac cttttgaagc acctactgtt acgtggtcac ctaaacgaat ttctccgcca
    29461 agcaaagcct cgctcaatcg atcttcaatt tctttttgaa gtgcgcgacg gattggacgt
    29521 gcaccatatt caggatcaaa tccagctttc ccgatcacat cgattgcaga tggtgtgatt
    29581 ttcacttgaa tatcttgttc tttcaatcgt ttgataatcg ctttgctcat gatttggacg
    29641 attgtatgga tttcatcttt actcaatgaa tggaagacga cagtctcatc gattcgattc
    29701 aagaattcag gtcggaaagc tttctttaac tcttctaaga tacgtttttg catggctttg
    29761 tgatctttag taacatcttg gacattgaat ccgacatttt tttcttctcg aatttgtgtc
    29821 gctccgatat ttgaagtcat aatcatgatt gtatttctaa agtccacttt tctaccttta
    29881 gaatctgtta aatgaccatc atccaatact tgcagaagta aattgaaaac gtctggatgt
    29941 gctttctcca cttcatctaa gagaatcact gaatatggtt tttgacgaat tttttctgtc
    30001 aactgtccac cttcatcata ccctacgtat cctggaggag acccgattaa tcggcttgta
    30061 ctgtattttt ccataaattc tgacatatca acacggatca gggcatcttc acttccaaac
    30121 atgacttctg aaagtgcttt ggccaattct gtttttccga caccagttgg tccaaggaac
    30181 atgaatgaac caattggacg atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca
    30241 cgtgaaactg ctttgacagc ctcatcttga ccaacgacac gttcatgcag aagtccttcc
    30301 agttctaaca agcgttcgct ttcttttttc tccaactgct gtagagggac accagtccac
    30361 tccgatacaa ctgtagcaac atcttcttct gttacacgat cagaataacc ttgctcctgt
    30421 tttgcttcaa ttgcagaaac tttttgcagc tttttacgta gtgctttttc ttgtctgcgt
    30481 aattgagcag cattttcaaa gtcttgtttt tggatcgctg cttccttttc ttgtactagt
    30541 tcatcgattt ctagtttgat cacagttgat tttgttaagt gatcggtttg atccaaacgg
    30601 actttcgctg ctgattcatc gatcaaatca attgctttat cgggtagttg acgagaagtg
    30661 atataacgaa cagaaagatt gactgctgct cgtacagctt catctgtgat ctccactcca
    30721 tggtgctctt catatcttgt acgtaatcct tgaagaatca cttctgcttc ttcaggagtt
    30781 ggctcatcta cttgaatgcg agcaaaacgt cgctctaatg cagaatcttt ttcgatatat
    30841 ttttgatatt catctagggt cgttgctccg atcgtttgta attctcctcg agctaacgcc
    30901 ggtttcaaga tattcgaagc atcaattgct ccttctgctc cacctgcacc aattaaggta
    30961 tgcaattcat caatgaataa gattacttga ccatcattat agatttcgtc gatgaccttt
    31021 ttcattcgat cttcaaattc accgcgatat ttcgtaccag caaccaaaga tcccatatca
    31081 agcatcatca ggcgcttttc ttgcatgtct tctggcactt cacctaaaat gattttttga
    31141 gctaaccctt cagcaatggc tgttttgcct acaccaggtt cacccaccag tacagggtta
    31201 ttctttgtcc gtcggctaag aatctggata agccgtttca cttctctcga acgaccaaca
    31261 actgggtcca gcttgttttc ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc
    31321 gtaggtgttc cttgcggtgt ttgccgttta tcttgtacat ttacatttct acgacgattc
    31381 attccttttc cagcagaatc accagctagc cccatttttt gtttcagaac ttgacgggct
    31441 tttgaaaggc tgatacctaa gttgatcatg attcgagtag ccatgatact ttcatctttt
    31501 aataagctca tcaaaatatg ttctgttcca actttttgag aaccagtttt cttcacttcg
    31561 ctgctagcta aagcaaaaac ttgtttcatt cttggtgaat atggaagaaa gactccttta
    31621 gggtaagaac ggataccacc ataaccaatc agatgttcaa tttcttcata aatttccgtt
    31681 tcattcaaat ccatttcacg taatactttt ccagcaattc cattttgctc gaccactaag
    31741 gctagtaaaa catgttctga actaactgat tgatgtttca accgttttgc ttcttcttgt
    31801 gcaatttcta atactgcttg ggctctttca gtaaaaagtt cttccatcta atctcacttc
    31861 ccatcttcat aacttaaacg ttccagaaat gctcggagaa tccgagcacg tgtatgatct
    31921 tcatattcat tgaaattcaa tgtgtttttt gcaattgcac tcaacatcaa gtttccttct
    31981 ttttttgtaa ttatttggtc ttcataaagt ttttgaataa tagcaaaagc atttttctca
    32041 ctgattgtat catcaaataa ctgatttatc tgctcaagta attgtttttt atctgaaatc
    32101 cttacttttg caattcgtat ataaccacca ccaccacgtt tgctttctac tagataacca
    32161 cgttggatgg taaaacgggt attaatcaca tagttgattt gtgaaggaac acagttaaag
    32221 aggtctgcca tttcaatcct tcgaatctcg atcatttcac tattttccag aattttcttt
    32281 atatatgatt caattaaatc cgaggtattc tgatgtgcca tcttattcat tccctttgac
    32341 taatattgac cttaattata acgaatttga taatagaaag aaagaagaac gctttttgtt
    32401 aaataaatcc tttagaatca acaaaaaaaa atacggaata tactattccg tatcgacgcg
    32461 cccaagagga gtcgaacccc taaccttttg atccgtagtc aaacactcta tccagttgag
    32521 ctatgggcgc atattcaatt taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata
    32581 aaaaaaatcg ggaagacagg attcgaacct gcgacccctt ggtcccaaac caagtgctct
    32641 accaagctga gctacttccc gtttaaaaaa taaaaaagac
//
LOCUS       DACDIH010000026        32535 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDIH010000026 DACDIH010000000
VERSION     DACDIH010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.026"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            133..507
                     /locus_tag="IV281_001292"
     CDS             133..507
                     /locus_tag="IV281_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8780314.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(621..1250)
                     /locus_tag="IV281_001293"
     CDS             complement(621..1250)
                     /locus_tag="IV281_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8780315.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1384..1456)
                     /locus_tag="IV281_001294"
     tRNA            complement(1384..1456)
                     /locus_tag="IV281_001294"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1421..1423),aa:Thr,seq:ggt)
     gene            1670..1743
                     /locus_tag="IV281_001295"
     tRNA            1670..1743
                     /locus_tag="IV281_001295"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1704..1706,aa:Asn,seq:gtt)
     gene            1698..1847
                     /locus_tag="IV281_001296"
     CDS             1698..1847
                     /locus_tag="IV281_001296"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780316.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2340..3668
                     /locus_tag="IV281_001297"
     CDS             2340..3668
                     /locus_tag="IV281_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8780317.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3870..4703
                     /locus_tag="IV281_001298"
     CDS             3870..4703
                     /locus_tag="IV281_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP8780318.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4719..5456
                     /locus_tag="IV281_001299"
     CDS             4719..5456
                     /locus_tag="IV281_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP8780319.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5611..6579)
                     /locus_tag="IV281_001300"
     CDS             complement(5611..6579)
                     /locus_tag="IV281_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP8780320.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6811..7644
                     /locus_tag="IV281_001301"
     CDS             6811..7644
                     /locus_tag="IV281_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP8780321.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7678..8517
                     /locus_tag="IV281_001302"
     CDS             7678..8517
                     /locus_tag="IV281_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8780322.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8543..9103
                     /locus_tag="IV281_001303"
     CDS             8543..9103
                     /locus_tag="IV281_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8780323.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9302..11023
                     /locus_tag="IV281_001304"
     CDS             9302..11023
                     /locus_tag="IV281_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP8780324.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11188..12330
                     /locus_tag="IV281_001305"
     CDS             11188..12330
                     /locus_tag="IV281_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8780325.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12346..13557
                     /gene="thiI"
                     /locus_tag="IV281_001306"
     CDS             12346..13557
                     /gene="thiI"
                     /locus_tag="IV281_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP8780326.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14130..14777
                     /locus_tag="IV281_001307"
     CDS             14130..14777
                     /locus_tag="IV281_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8780327.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            15170..17815
                     /locus_tag="IV281_001308"
     CDS             15170..17815
                     /locus_tag="IV281_001308"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP8780328.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18125..19438
                     /locus_tag="IV281_001309"
     CDS             18125..19438
                     /locus_tag="IV281_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP8780329.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19428..20081
                     /locus_tag="IV281_001310"
     CDS             19428..20081
                     /locus_tag="IV281_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8780330.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20136..20828
                     /locus_tag="IV281_001311"
     CDS             20136..20828
                     /locus_tag="IV281_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP8780331.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            20978..21178
                     /locus_tag="IV281_001312"
     CDS             20978..21178
                     /locus_tag="IV281_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8780332.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(21432..23111)
                     /locus_tag="IV281_001313"
     CDS             complement(21432..23111)
                     /locus_tag="IV281_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8780333.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(23113..23325)
                     /locus_tag="IV281_001314"
     CDS             complement(23113..23325)
                     /locus_tag="IV281_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP8780334.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            23718..25448
                     /gene="efrA"
                     /locus_tag="IV281_001315"
     CDS             23718..25448
                     /gene="efrA"
                     /locus_tag="IV281_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP8780335.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            25445..27205
                     /gene="efrB"
                     /locus_tag="IV281_001316"
     CDS             25445..27205
                     /gene="efrB"
                     /locus_tag="IV281_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP8780336.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            27300..27497
                     /locus_tag="IV281_001317"
     CDS             27300..27497
                     /locus_tag="IV281_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780337.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            27650..28135
                     /locus_tag="IV281_001318"
     CDS             27650..28135
                     /locus_tag="IV281_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP8780338.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(28239..28832)
                     /gene="clpP"
                     /locus_tag="IV281_001319"
     CDS             complement(28239..28832)
                     /gene="clpP"
                     /locus_tag="IV281_001319"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP8780339.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            29012..29539
                     /locus_tag="IV281_001320"
     CDS             29012..29539
                     /locus_tag="IV281_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP8780340.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            29630..30367
                     /locus_tag="IV281_001321"
     CDS             29630..30367
                     /locus_tag="IV281_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8780341.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            30371..31288
                     /gene="miaA"
                     /locus_tag="IV281_001322"
     CDS             30371..31288
                     /gene="miaA"
                     /locus_tag="IV281_001322"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP8780342.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            31290..32519
                     /gene="hflX"
                     /locus_tag="IV281_001323"
     CDS             31290..32519
                     /gene="hflX"
                     /locus_tag="IV281_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP8780343.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10771 a         5522 c         6876 g         9366 t
ORIGIN      
        1 gtctacactc tgagatgtaa gaaatatcat aagatatttc ttacatcttt ttgttttgag
       61 caggacttta gataatttta ttaatatatg ctagcatgct tttgtaagaa aaagaaagag
      121 ggcgtattag ttatgtatct taaaattacg gaacaagcac aagaaagatt acaaaaatat
      181 attgatgaag gcgcaacagt tatattggat ttagatgatg gcgttgggga atattctaaa
      241 atgggtgttt gctctttaga tactagtttt cgtttacttc ttcttgataa agaacagaga
      301 aaaaatgatt ataaactcca attagatagt gatattgggg atgtttatat aaaagattat
      361 tcaaaaatgt acatggatga acatatgacc ctttctttag atccacgtct aggtgtattt
      421 actcttgaaa gcccaagtgg ttctttagac agtcatgttc aattagtaga tctacgacca
      481 gaaaaattct ctggaataaa acaataactt tctctctatt gtccataaaa ctagtctcaa
      541 tcaagctgta ttgaaaaaac aagacaacat tcttaccatg gtaaataaaa agacgacaca
      601 ttttgtcgtc tttttttaaa ttaactattt tcgataaaag atcgcatagc ttgtgccatt
      661 atttctggct tttctctatt caattcatgt tttccgtttt taataaagag tagccggcca
      721 ttcgttgaaa gtttatataa acgttttgct gctggtttat tcgcatgatc tttggtaccg
      781 cataaaatca atttaggaag tcgtgatgta cgaagctgtt ttttgcagtc taatgtttca
      841 agtgaacgaa gtagggtaag ggtttgtttt ttagatagac ctaatttttc aaatatctta
      901 gaaggtacgc agtaaaaata aatattctgg aaagtaagca ataagcgatt aggagactca
      961 aattgtggag cgactaaaac aacctttttt aaatatggag aaggagttgc aagatagttt
     1021 aaagcaataa cagcgcctaa agataatcca cataaaataa aaggttcctt tatctgattc
     1081 aattgtactt cgatgtattt ggttaagtcg tctaaagtag cagtgtctgt gctgtttaaa
     1141 tgattgaaca atgaaagact tttgcaagaa taactattgg gtagataaga gattacatct
     1201 ttccatgatt gttcgtcttg gcctagacca tgcaaaaaaa caattttcat attgctctcc
     1261 tttttatagt tatcatatag actttctttg tactattaaa atataaacat tcatttctta
     1321 cgtcataaaa taaaaaaaca gcgcagacac attgttaaac caacatgttg cactgtttaa
     1381 ggaagccact tgccgggctt gaaccggcga cctcttcctt accatggaag cgctctacca
     1441 gctgagctaa agcggcatta tcattaatac aaataatagg ataacgaata ttaatgtatt
     1501 tgtcaacaga attttttcga aaaaatgtgt ttctctaata ggaaaacaga ttttagaaaa
     1561 aataaaaata acaaaaaatg cgaacggttt ctttgataat ataataaaaa aattcaaaaa
     1621 aagtattgcg ttatttagaa gaaattgata ttatattcat gtacttgatt ccggcatagc
     1681 tcagttggta gtagcgcatg actgttaatc atgatgtcgt aggttcgagt cctactgccg
     1741 gagtaaataa agcttatgca aagaaaaaga aatccaatca cttgggtttc tttttctttt
     1801 attattatga tgaatcaagc tttctgtttt tgtcacgtat gttgtaatct ttacgaaaga
     1861 gtagagttgg ctactcataa tcaaccatac ttcccatcat cgctaaatac gaaataaatc
     1921 agcattccta caccaatcaa agcacaagct gcaccaagaa aagggatgac attcccaaag
     1981 ttatctgtat tcataagtat gccaccaccc acaaacaaaa agccgccagc aatcaggttt
     2041 ttcattttat tcatctcctt tcttacgtta tagtatttcc ttctttcaat gagtattttg
     2101 aaagctatct tattgtatga tgttcttcta ggcctcttct ttattataca ggccattttc
     2161 gttatctcat agaaatattc ctttttatct gaaaactatt gtataccgta tgacattttt
     2221 cttcatgaag ttgtaagtat attctcattc aaatgaaaat ttcatcatag aagagattgt
     2281 atttctaaat tcttaagagt aaaatacgaa gtataacttg gatttggaag gatgattgga
     2341 tgaaagctat tacgcctgat atgacaacaa aatttaacaa acagtttcaa gcaaatatca
     2401 aacagcaggc attgcagcgg gcagttgtga agaacggaat tactgcttct gctgaaaatg
     2461 tttcagcaca tgtggagaat acaccggtat tttctataga tttggctacg gggaaagtgg
     2521 ctaatcaaat gcaatcgggg cgttgctgga tgtttgctgc actgaacaca ttccgtcata
     2581 agatgctgaa tcaattccat ttaaaagagt ttgaattatc acaaaattac acttttttct
     2641 gggataaata tgaaaaggca aattatttct atgaaaacat cattgcaaca ggaaaagagg
     2701 caatcgatag ccggaaagta gctttcttac tcgctactcc tcaacaagat ggcggccaat
     2761 gggatatgat cgtttcatta ttccaaaaat atggcgttgt gcctaaatcg gtgatgccgg
     2821 aaagtgcgaa tagttcaaat tctcgagatt tgaataacta cttgaacaaa ttactgcgta
     2881 aagatgcagt agtgttacgg aaaatggttg ctcaaggaga aacattagac gctattgaag
     2941 aaaagaaaga agaaatgcta gaaagcattt ataacttctt agctatctca ttaggaacac
     3001 ctccaaaaga attcgatttt gagtaccgcg atgaagaaaa gaactatcat cttgaccgag
     3061 gactgacacc acaaattttt tatgataaat acatcggtgt aaaattagat gattatgtta
     3121 gcgtgatcaa tgcaccaaca aaagacaaac cattcaatcg ttcttataca gtagagatgc
     3181 taggaaatgt cgttggcggg aaagaagtca aatatctgaa tgttgatatg gaaactttca
     3241 aaaaattagc gattgctcag cttgaagaag gagaatctgt ttggtttggt tgtgatgtag
     3301 gtcaatcttc tactagaact agtggcatca tggccttaga tgcttattca atggatgatc
     3361 tatttgatac ggattttaca atgactaaag ctgaacgttt agattttgga gaaagtctga
     3421 tgacccatgc gatggtattg accggtgttg acttagtaga cgggcaatca acaaaatgga
     3481 aagtcgaaaa tagctggggc gaaaaagttg gaaagaatgg cttctttgtt atgagtgatg
     3541 cctggatgga cgaatatacg taccaaatcg ttgtaagaaa agatttattg acgaaagagc
     3601 aattaaatgc gttcaatgaa gaaccaattg ttctatctcc gtgggatcca atgggtgctt
     3661 tagcatagac gagtatttca atgaagagta tgtagtaaag ataaaaagag ctggcttaag
     3721 ccagctcttt ttatctttaa agtccattcc ctgtttttta tggtccctgt gctataattc
     3781 taaataaagg ataaggattg gaaataatcc ttttatcatt aaaaatatga tgacgaagta
     3841 gaataaaatt gggagtaggg gggaaagtca tggatatcca aactgtttta tctgatctga
     3901 aattaaatgg taaagtgatt ccagttgttg gtggcgatgt taatcagacc tatcgtataa
     3961 aaaccgaaca tcgtgcttat tttttaaaaa tccatccgaa tgtaaaaaaa ggattttttg
     4021 aagcagaagt agacggatta aaagaactgt cagcattcgt acgtgtaccg gatacgtata
     4081 tgctaggtga aacaagtgaa ggcgcttatt tgttaatgga atggatcgag ccgggaaaag
     4141 gggatcaacg agatcttgct gcagctttag ctaatctcca tcagcaaaca gcgccacaat
     4201 ttggttttcg taaagataac tatttgggta cacttgttca gaaaaattct tttgaagaag
     4261 attggtggac ttttttcttt aaagatcggc ttgaatccca gatttctttg gcagaagaaa
     4321 cgaatcgatg gaatgtacag cgtcaagaaa agtatctgcg atttaaagaa cgtgtattga
     4381 aatcggttga accaaaaaag atcactccac gcctcttgca cggagacctg tggagtggta
     4441 atgtgttctt tgaccagcaa gggcatccag tttttgtcga cccagctgtt tcttatggga
     4501 atcgggaaca agatattgca atgagccagt tattcggtgg tttccgaccg gaatttttag
     4561 atgcttatca aactatattt ccattagaaa agggatggaa agaccgactg ccgatttacc
     4621 agttatatta tcttttggca catttgaata tgtttggaga aagttacggt tcccaagtag
     4681 atcaattact agaaaatttt taagaggagg gcgtgttttt ggcaaaaaaa tataccaaaa
     4741 agcatgaagt ttcatattat gagtgtgaca tcaaccaaac gatgacattt ccttcgatgt
     4801 taggagtagt tatcaaaacg tctgaagatc aaagcgacca gttgggaaga ggcagtgaat
     4861 tcgtcaatag ctttggactt acttgggtca tcacgaatta ttccatggag attaatcgtc
     4921 tccctagagt cggtgaagta atctcagtaa cgactcaagc catggagtac aataagtttt
     4981 tctgttatcg gaacttctgg atccatgatg aagatgggaa agaattagtc aaaatagaat
     5041 ctgttttcgt attgatggat tttgtgaatc gaaaaatgag cagtgttaat gaagagatca
     5101 ttgctccttt tgagagtgag aagatcaaga aaatcaagcg tcaggaaaaa attgaaaaga
     5161 ttgattcggg cttcatgttg ccatatcgtg ttcgttttta tgatatcgat agtaatcagc
     5221 atgttaataa tgcgatgtat tttaattgga tcattgacgt attaggatat gactttttga
     5281 ctacgcatgt tccagaaaaa gtaattattc gatttgataa agaagtagag tacgggaatg
     5341 agatcgaaag ccattttgaa caaatcccta tcgaaaatgg tgtcaaaaca aaacatgaaa
     5401 tccgaatgca agaacagctg tattgtgaag caaatattat atggaaaaat aaataaaaaa
     5461 gaggttgtga cagaaggggg caacttcaag aaataagaag aaatttacga aaattgtttc
     5521 tcaaattttt gtaaattttc gacttagttt tcgaagaagc tatttttgtc acagcctctc
     5581 tttttaagtt tagctattag ataatgatgt ttagttttgg ataaacgaaa tcaattcttc
     5641 acccgacaaa ccatagttta cagcaatcaa tagttttaaa cgtgcttttt gactattaat
     5701 actgttacag aaaataatcc ccattttttc tagttctttt cccccgcctt cataatcata
     5761 aacaggctct gcgattccat tgaaacaacg agaaacaagt actacaggga ttttttggac
     5821 aagaagcttt tccaaagcag ctaaggcttg tggagggaga ttccccgcac ctaaagcttc
     5881 gattaccaaa ccgtctactt tcgtatgggc gatggcttcc aataagtccc cttgcatccc
     5941 agcatatgct ttaacgatcg ggatagtccc atctacagcg gaaatgtcca gtcgctttgt
     6001 ttctaaaagt tcctgcaaga ataaaattcg attcttagtt actaagccga tgggacccaa
     6061 tgtaggtgtt cggaaagtag caacatttgt ggtatgggtc tttgtcacgt agcgtgcaga
     6121 atggatctca tcgttcatga ccaccaaaac ccccttatct agcgcttctt cacaacttgc
     6181 gacacgaatc gcactttcaa aattataaag gccgtcactt ccaagttcat tacttgaacg
     6241 cattgcccca gtcaaaacaa ttggtaattt gttgccaatc gttgtatcaa gaaaaaaagc
     6301 agtttcttct aatgtatctg taccgtgtgt gatcaccact ccggatatac ctgatgcata
     6361 agcggacaag atacgttctt ttaataccaa catgtgagaa ggcatgatgt gtggtgaggg
     6421 aagattgaag atatcttcaa ctaataattc tacatgcttg gggattgtga tttctgcatc
     6481 taataatggg tttgccacat caggactaac accgcccgtg agcatgtttt ctttcatggc
     6541 gatcgttccg ccggtgtgta gtacaagtat ttttttcatt ttacagactt ccttacgaat
     6601 aattttgcat agccgttaaa tcttattgta tactatataa aagagttttg ctatcagtga
     6661 agaataacga atagcctaac ttatcataaa aaagctgacc ttattaaaat ggaaaagact
     6721 caaccttgac ctcctaatac aatggagtat ctggttgagt ttcttttaca agaaagcagc
     6781 caaggaaatc aatcacgaag gtggccggaa atgatcagaa tcggattaac atcgtttaaa
     6841 gaacatgaca aattaactgg taaaaagaat aacactcttt atgaatatgc agggtttcta
     6901 cccttagtgg aattagatac tgcctattat ggaattcccc gaaagtcctc agttgagaat
     6961 tggataagtg aagtacctga atcatttcgc tttgtagtca aagtctatca aggaatcagc
     7021 ggtcaaggag actggaagcc ttattatcag accgaagaag agatgattgc gcatttttta
     7081 gatgttatgt cgccaatggt agatgctgga aagatgtttt gctttttagt ccaattcccc
     7141 tcatcattca aatgtacgaa agaaaatgtc cagtacttgc gcaaacttag aaagtggttt
     7201 gagggttatc ctgtttctat agaattgcgg gattattctt ggtacagcga tgaatttctt
     7261 gaacgtacaa gaaactttat gtctagagcc gctttttctt tagccatcat cgatgagcca
     7321 cagttgctga atacaacgat tccttttgat ccatttgtca ccaatccgaa ctttgctctt
     7381 tttcgattcc atggtaggaa tgcgcaagga tggcaagcaa atgataaaga gtggcgtaaa
     7441 aaaagaacgt tatatcgtta ttcagaaaaa gaactgcaag aactagcaca tgaagtcaaa
     7501 gtagttgatt ctaagacaaa agaggtaggt gtgattttta ataataattc tggtggtgat
     7561 gcggctgaaa atgcattagc actgcaaaaa atattaaata tcaagccaga tggactcaat
     7621 ccaacccaaa tggatctgtt ttaagggctt attttattca agaaaggggc gaagtctatg
     7681 atcaaagcta ttttttttga tattgacgga acattggtca ccaatcgttc aaaagcgttg
     7741 gaatcaacaa aacaagcaat tgaatatgct cggaaaaacg gcattttatg cggtgtagca
     7801 acggggcgca gcccagtaaa gatcaaagaa atcattgatg aattggaatt agatatgtat
     7861 gtcgtgtata atggtcaact ggtttttact gcagatcgca cgattatcga ccaccctttt
     7921 gaacaaaaag tattggaaca tattgttgaa tttgcagatg agaaccatcg acaaatcgtt
     7981 tttggtgcga gaaatcggtt agatggatca acgacgatgc tattaggaca atcgattttt
     8041 atcaagcgac tagtcagctt tttaccgcgt aaattcccgg tacgtctgat gaaaaaaatc
     8101 ctccaggttt ttagccctca ccgacaaaag gatcgttatg aaacgttaga gatattaaaa
     8161 gaaccaattt atcaatgtat attgctcagt ccggagtcag aacagaaaaa gttagaacat
     8221 cgctttcccg agtgtacgtt tcagcgttca aatacgtata ctgtagatat catcccaaaa
     8281 ggtggttcaa aattactagg catccaagct ttcgcaaatg ctgtcgaaat tgaaatggaa
     8341 gaaattatgg cattcggcga tcattacaat gatatcgaga tgctgaaagg tgtaggaatt
     8401 ggagtggcca tggggaatgc tcagatcgaa gtaaaacatg ctgcagactt tgtgactcaa
     8461 tcaaatgaag aagatgggat atacgaagga ctgaaacact tcggtctgat ttattagtca
     8521 atgaaagaag gaaaaaatca gtatggaagc agagacacct tttcaaaaag tagaaacatt
     8581 ccacaaaact tttgatccaa gaagaccaga gatacccacg gcttttacag aaaatgaagc
     8641 tcatacacgt gctgcgttca aaatagaaga acttgttgaa tttgttgcag ctacttcacc
     8701 taataaagaa caactgcatg aagctgttat gctgctgcac gaagcattag ataaagcaga
     8761 agaaaaaata cttggtaaaa cacagtgggg aagtgatccg ctggttgaac aagtagatgc
     8821 actgacagat atcctttact ttacttatgg ttctttttca ttactaggaa tagtgcctga
     8881 tcgaatattc gaaatcgtcc atcaggcaaa tatggggaaa ttatttccag atggaaaacc
     8941 tcattatgac cctgtaacaa ataaagtgtt gaaacctgaa aactggcaga tggaatatgc
     9001 tccagaacct aaaatcgcgg cagaaatcca acgtcagatt caagaagcga ttgctaagaa
     9061 gaaatccgct gatataggca tgtgcacaaa tgaaaaagag tgatcagagt ttcactattt
     9121 ttgttatctt tatacgatgg aaaaacaaaa gattttcgtt taagttagtg aatgcatgaa
     9181 aatagtatgc tataatctag aatagatgtc ttttgcacga gagacgtatt atttggattt
     9241 atgccaagca gaacgccatc tgttgcataa aagatgcata ctgaaaacgg aggaaccata
     9301 aatgggaaat aatgttatca tcggaatcgt cattgcaatc attatagtcg ccattgtcgt
     9361 ctttttggtt agttacttga tgaagaaaaa aaaccaagat cgattggatg ccttagaaaa
     9421 acgcaaggaa gaattatttg acttgccagt gattgaagaa gtcgatgatg ttaaaaaaat
     9481 gcatctggtt gggcaaagcc aaaatacctt tcgtgaatgg agtcaaaaat ggaatgacat
     9541 atctacttcc tcgttcgctg aactagaaag tcaaattttc gaagtggaaa acctcaatga
     9601 aagttttcgg ttcttaaaag gaaaaaaagc agttgttgaa gcagaagcta caatggacca
     9661 aatggagaaa caagtgacgg aaatccgtga aggtctaaag gaattgcggg aaacggaaga
     9721 acgcaactca ctagctgttc agcaggcact tgacgtctat gaagaactga aagaaaaagt
     9781 cagagaagac gagcatgaat acggaccagc tctttttgaa ttgcaaaaac agatcaaagt
     9841 gatcgaaaaa gaatttacgc agtttgtgac tttaaacact tctggagacc ctgttgaagc
     9901 ccgcgaggtt ttggataaag cagaaaaaca tacgtatgaa gtggaagctc tgatgaaaaa
     9961 aattccaccg ctgtatgagg agctccatac cactttccct gaacaacttg aagaaatcag
    10021 tgatacttac gagaaattga tggaagaaca atatgtattc ccagaagaag atcttgctga
    10081 agatatcgca aaagtaagtc gaagaattga aaattcatta gccaatcttg aaaaaacaga
    10141 agttgaaacg gtcgaatttg aaaaccgtga aacagctgat ttgattgatt cgttatatga
    10201 tatcttagaa cgggaaatgg aagctcagag atatgtaaaa actaaccaat caacgattgc
    10261 agagtatatc aaacatacaa caaagaataa ccgtcaatta ttgattgagc tggatcacac
    10321 atctcaaagc tatacattga accacaatga aattggccgt gtacgaggtt tccaaaccga
    10381 agtggaagaa atggaacgtc aaaatgaaca gatgattcca cagatccgtc aacacgaaat
    10441 tccttattca gaagtgcgca cgttctataa aacagtcttc aaagtcttag aagatatcga
    10501 aaagcaacaa gtagagatcg acgactcatt acatgaattg agaaaaggcg aaaaagaagc
    10561 gcaagaaaag atcgatacat tcgaattcaa attgcgcagt ttgaaacgtt ttgtagaaaa
    10621 acaacgtttg cctggcttgc cgaatgatta cctagaattc ttttttgttg ctactgaccg
    10681 tattgaagaa ctaagtgttg tcttgaataa aattcgagta aacatggaag aggtcaatcg
    10741 tttagttgct ctatgtgaag aagatctaga attattagat aagaagacac atgatttagt
    10801 agatgcagca gcattaacgg agcaaatgct ccaatatgca aatcgttatc gtcatacaca
    10861 cgaagaagtg agagaagcaa ttgataaaag ctactattta ttcaataaag aatatcacta
    10921 tcaagaagca ttagacgaaa tcggtacagc attagagcga gtagaacctg gtgctttcaa
    10981 acgcatcgaa gatttttatt ttaatcatcc agacttagta taaagcaaaa aagttagtaa
    11041 accagcaaga gcaagacgga ttattgtctt gttcttgtct ttttttgtat atttgaaaat
    11101 tgaaacaagt gaaaatttca cctgctcttg aaagcacctt ggacaaccgt tataatagaa
    11161 aagaatttca aaaaagaggg gtttcttatg atatattttg ataacagtgc aacaacccca
    11221 atctttccgc aaagcttaga tgcatatgta aaaaccagtc agcggattat tgggaatcca
    11281 tcaagcttgc atgatttagg caatcaagct aaccggttgt tgcagcaagc aagaaagcag
    11341 atcgctgatt tgatccatgt agaaccagaa gaaatctatt ttacttcagg cggaacagaa
    11401 ggagataact gggtattaaa aggaacgatg atcgaaaaaa gagaatatgg gaatcatatg
    11461 atcatttcag gagtcgaaca tccggccgtc agcgagacag cagaacaatt aaaagaatta
    11521 ggaatcgaag taagtatcgc cccagtggat aaacgcggat ttgtacgtgt cgacgaattg
    11581 aaagaattaa taagaaaaga gacagtatta gtctctgtta tggctgtgaa taatgaagta
    11641 ggatcgattc agcccatcca ggaaatcagt gatcttttgg atgattttcc taaaattcat
    11701 ttccatgtag atgctgttca ggcgattgga aaagtaccat tctcgcaatg gttgacagaa
    11761 agagttgatt ttgcaacatt ttcagctcat aaattccatg gcccacgggg aactggtttc
    11821 atttattgga aaaagggtcg tcgtttagca ccattactga caggtggagg gcaagaaaga
    11881 aatcagcgaa gtgggacaga aaatgttcca ggtattgtgg ctatggttaa agctttacgt
    11941 ttatcattag agataaagga acaaaaacct gagcagacac aacgcttaaa agtagctcta
    12001 atcaatgcgt tgaagacgta tgagaaagtg acgatttttt cagaaggaac tgactatgca
    12061 ccgcatattt tatgttttgc tctaaaagga attcgcggcg aagttctcgt ccatgcctta
    12121 gaagaaaaac aaatatttgt ttctactact agtgcctgct cctcacgtaa acatatggca
    12181 agcagtacat tgcacgcaat gcatgtacca aatgatctgg ctacatctgc tgttcgaatc
    12241 agcttagatg aaaacaattc gcttgcagaa gtcgagcaat ttatgattat tttcaagcag
    12301 ctttataaga aattttctgc aatcaattaa aagaggagtg ttcttgtgaa atacagtgaa
    12361 attatggtcc gttatgggga actttcgaca aaaggaaaga atcgaaaaag ctttatcatg
    12421 caactagcac aaaatgtcag acaagctttg aaagactttc cagaaatcaa aatccatgct
    12481 gatcgggacc ggatgcatct tttattgaat ggcgcagacg ggcagctagt tattccaaaa
    12541 ctagcaaaaa tatttgggat ccaaaatttc tctccaagta ttcgtgtaga aaaagatgtc
    12601 gaagttttga aacaagctgt tcaagacatc atgaaagaaa tttatactgg gaaagaaaca
    12661 tttaaaatta tcgctaaaag aagcgatcac caatttgaat tgacaagtaa tgaattgaat
    12721 caaacattag gtaatgctgt ttttgatatt ttcccgcata tccaagttca aatgcgtcaa
    12781 ccagatattc cattacgtgt agagattcgg cgtgatggcg cttatctatc gtatgaaacg
    12841 atcaagggag ctggaggatt gcccgtagga acaagcggaa aaggaatgct tatgctttct
    12901 ggggggattg attctcctgt agcagggtac tttgccatga agcggggagt ggaaattgaa
    12961 gctgttcatt ttgctagtcc gccgtataca agtgaacaag cactgcaaaa agcaaaagat
    13021 ctaacagcca aactagcacc ttatgtggga acgatccatt ttatcgaagt gccatttacc
    13081 gagattcaag aagaaatcaa aaagtcagtg ccacaaggat attggatgac aattactcgt
    13141 cgaatgatgt tgcgtttgac cgatgaaatt cgccgtattc gtcatggttt agtcattttg
    13201 aatggtgaat cattaggtca agtggcttca caaactttgc aaagtatggt ggctatcaat
    13261 gaagtaacga atacgccaat cattcggcct gtagcgacaa tggataaact tgaaatcatt
    13321 gaagtagcag aacagatcga tacatttaat ctttctatcc agccttttga agattgctgt
    13381 acgatttttg ctcctccaca gccaaaaaca cgccctcaac tggaaaaggt ccatcaatat
    13441 gaagagcgtt tagcaataga tgaaatgatc caacgagcac tagctggctt gaagatggaa
    13501 gagatacaag cagaatcgtc taatgaaaaa actgaagaat tttcagaatt gctataagag
    13561 gttatgaaaa tagtgttttg accagagtat tagagaaaca aaacgaaaaa atagctccat
    13621 gtattttacc gtttttgaac taatgtcgaa gattagctga ttgaatacca tttatcaaga
    13681 agttatggaa aagccggttc ttttttacta actgaaaaat aaaattttat ttagagatca
    13741 agctacagtt gataaatgat ataaaaaaga gaatttccca tgaaatagcg aggttgtgac
    13801 agaagtgggc agcttcaaga aataagaagg aatttacgaa aattgctttt caaatttttg
    13861 tgaaatttcg gcttattttt cgaagaagtt acttctggaa caccgtgtat cagaggttgc
    13921 gaaaaagctg ccctgcatca agtaataaga acagccaaac aaaaatagtt cctcatattt
    13981 tacgtttggt tgagcttatt accgcagatg caagcttttg aacaccgttt tatttgtctc
    14041 acaccctttt ccttcctact tgtaaccttg ctcccgtgtt agaaacatgt tacactaaat
    14101 atgtgaaatt attaacaagg agcgtcgtta tgcgagaaaa aacagataca ctgccaaaag
    14161 caactgcgaa aagattacct ttatatttac gctacttgaa gatgctggat gactcaggta
    14221 taagtagaat caaatcaaat gagttcagcg aaatcaccca aattccttct gcaacgatcc
    14281 gcagagactt ctcgcaatta ggcgagctag gcagaagcgg atatggctat gatgtccctt
    14341 ttcttatcga tgtattcaat aatatcctta atactaaaga agaaaaacga atcgcattag
    14401 taggatatgg aaatcttggc aaagcattga aacacaacaa ttttagaaga aatgaaaatc
    14461 taaatatcgt gtgcgtcttt gataatgatc cagcactgat caatcgagta attgatggag
    14521 aaatgatcta tccgattgat cgtttcgcgg agatagccaa agcaaaaaat gtgacagtag
    14581 caatctctac ggtaccaagt aaatattcac aatctgctat tgatgaaatt gtcaaaggaa
    14641 atgtaaccgc tatacttaat tttgcgcctg acagagtaac tgtcccagct tatgtcaatg
    14701 ttcaatacat tgatttgaca accgagttgc agacgcttat ttactttgat gagaattact
    14761 cagaagtttt ttcttaggaa aatttgagta ggcttattga tcttgcgaga actcgtcgta
    14821 attctttcaa aaatttttta gtactaaatg aagtggtttt ttcacgacca cttgttgact
    14881 aaaaaaaaaa accattgtag aatgaagcat aacgctgaac aagaggagta actggcacat
    14941 tcattcttag agagagaact atctgctgaa aggttctcaa tgacaaccag tgaaggtagc
    15001 ttgggagttt ctgtcttgaa tggagtagag atagacgagt cgtgatcgtt aactcacttg
    15061 agaggcaata atgattgatt attgcaattt aggtggtacc gcgtaaatca tacgtcctag
    15121 aatacgaaag tattttagga cgttttttag tttaaaggag gaaaacagca tgacggaaga
    15181 aaataaccta tcaacaaaat ataatccgca agaagttgaa gctggacgtt atcagcattg
    15241 gttagaacaa gatttgttta aaccgagcgg agataaaaaa gcaaaacctt attcaatcgt
    15301 aatcccgccc ccaaatgtta ctggaaaact tcatttaggg catgcttggg atacgacctt
    15361 gcaagatatg ctgattcgtc aaaagagaat gcaaggatat gatactttat ggctgcctgg
    15421 tatggaccat gcaggaatcg cgacacaagc aaaagtagaa gcgaagctta gagagcaagg
    15481 gatttctcgt tatgatctag gtcgtgaaaa gttcatcgat caagtgtggg aatggaaaga
    15541 agagtatgcg agccacatcc gcgaacagtg ggctaaaatg ggcttatctt tagattacag
    15601 tagggaacgt tttacattag atgatggatt atctgaagca gtacgtaaag tttttgttac
    15661 cttatacgaa aaaggtttga tttatcgtgg tgaatatatc atcaactggg atcctcaagc
    15721 acgaacagct ctatctgata tcgaagttat tcataaagat atcgaaggcg ccttttatca
    15781 tatgagctat gaactagcag atggatcagg cgtagtagaa attgcgacaa cccgtccaga
    15841 aacgatgttg ggagatacgg cgattgctgt tcatccagaa gacgagcgtt atcaagcgct
    15901 catcggcaaa aaagtgattc tacctttagt caataaacaa atcccaatca ttgcagatga
    15961 atatgtagat atggaattcg ggacaggtgt agtaaaaatc actccagcac atgatccaaa
    16021 cgactttgag gtaggtaatc gtcatgatct tccacgcgta aatgttatgg atgatgatgg
    16081 tacgatgaat gaactagcag gaaaatatgc tggaatggaa cgttttgctg ctcgtaaagc
    16141 aatcgtgcaa gatctgaaag aaatcggtcg attgattaaa atcgatacga tgatccacag
    16201 tgtgggtcat tccgagcgga caggtgtcgt tgttgaacct cggctgtcta ctcaatggtt
    16261 tgtaaaaatg gcgccgttag cgaaaaaagc cattgataac caagaaacag atcaagcagt
    16321 ggaattcttc cctccaagat tcaatcaaac gtttttacgt tggatggaaa atgtccatga
    16381 ttgggtaatc tcacgacaat tatggtgggg acatcaaatt cctgcatggt atcacaaaga
    16441 aacaggtgaa atgtatgtag ggatggaagc accagcggat agtgaaaact gggtacaaga
    16501 tcctgatgtt ttagacactt ggtttagttc agctttgtgg cctttctcaa caatgggctg
    16561 gccagatacg gaaagcgaag attataagcg atacttccct actagcactt tagtaaccgg
    16621 ttatgacatc attttcttct gggtaagcag aatgattttc caaagcttgg aatttactgg
    16681 cgaacgtcca tttgagaatg tcttgattca tggattgatt cgtgacgaac aagggcggaa
    16741 gatgagtaaa tcattaggta atggaatcga tccgatggaa gtaatcgaaa agtatggagc
    16801 agatgctctc cgctggttct tatcgaatgg ttctgcacca ggacaagatg tccgcttcag
    16861 ctatgaaaaa atggatgctt cttggaactt tatcaacaaa atctggaatg cttcacgttt
    16921 cgttatcatg aatatcgaag gaatgaccgt agaagagatc gatttatctg gagaaaaatc
    16981 agtggctgac cggtggatcc ttactcgttt gaatgaaacg atcgaacgag taactgagct
    17041 atttgatcgt tttgaatttg gtgaagcagg tcgacaactg tataacttta tctgggatga
    17101 tttttgtgac tggtacatcg aaatgagtaa agaagtactt tatggagatg atgctgtttc
    17161 aaaacaaacc acccgtagca tcttagtcca tacacttgat cagatcctac gtttattgca
    17221 tccaatcatg ccattcgtta cagaagaaat atgggaacat attcctcatc agggaaattc
    17281 tctagtagtg gcagaatatc ctgttgtacg tccagagttt gatgatgaaa cagcttctaa
    17341 agggatggaa gtattgaaag aattgattcg agccgttcga aatattcgtt cagaagtgaa
    17401 tacaccattg tctaaaccaa ttacgttgct aattaaaaca aacgatgcta aaatcgatca
    17461 atttttagtt gaaaatacaa gctatatcga acgcttctgt aatcccgaag agttgacgat
    17521 atcaagtgag atcactgcgc cagatctagc gatgtcagct gttctgacag gtgcagaaat
    17581 cttcttgccg ttagcaggat tgatcaatat tgaagaagaa atcaaacgat tagaaaaaga
    17641 actggaaaag tggacaagcg aagtaaaacg tgtccaagga aaactttcta atgaacgttt
    17701 tgtttctaac gcgccggaag aagtagtaga ggcagagcgt gcaaaagaaa aagattattt
    17761 agagaagcaa acggctgtca aagaacgaat cgaaagtttg cgtactattc aataagaata
    17821 gaaaaagaga gtgtgaaatc agctagctat tactaaaagc tagctgattg aacatcgtta
    17881 caggaaagag tttgagcctc actcttagct tcgattcata aggagatgtg acataaattt
    17941 tgtcgcatct cttttttagt ctactcttat tatgtaggag aaggagggag gtggatcatg
    18001 tatcaagagt caataaagta gttgataaaa tcgttcctct ctctgtttct tttctctttg
    18061 tcatactttt atttttctta tgtgttctat ttgttataat aaaagagaaa tgggggaatt
    18121 gttgatgacg attgaagaag caatagaatg gatccatagt cgattaccat ttggttcacg
    18181 tcctggcctt gaccgagtcg aagctttatt agaagtggta gggcatccag agagaaaagt
    18241 aaaaacgatc catatcgctg gaacaaacgg aaaaggttct actgtagctt atttgagaag
    18301 cctattagaa gaggctgggc tgacagtagg tacatttact tcaccttata tcgaaacgtt
    18361 caatgaacgg atcgcaatca atggcaaaag cattccagac aatgatttga tccagcttgt
    18421 aaaaaaatat cagccactga tcaaacaact tgatcaattt gaagcagtag ctggtattac
    18481 tgaattcgag acattaacag caatggcatt tgattatttt ttgatgaaga aagtggacgt
    18541 agcgattatc gaagttggct taggcggctt attggattct accaatgtag ctgtaccaat
    18601 gctgacggga ataacaacga ttggacttga tcataccgaa atacttggag atacaattga
    18661 agaaatcgct gcacaaaaag ctggaatcat caaacaaggc gttccagtag taacaggaaa
    18721 tattgcccca gaagctttta ctgtcatcca acaaacagct aaagcaaaaa atgcaccgct
    18781 ttatgtatac ggggaaaatt ataaaataaa ctatctgcat cctgatgaga tgtggggaga
    18841 agtatttgaa ttttttaatg aaataggaaa gatccctcaa ttaaccactc cgcttttagg
    18901 gcaacatcaa gctgaaaatg caggagtagc catcgaattg tattttgttt actgtcaatt
    18961 ggaaaagctg cctttccgag aaaaagatgt tttgaaggga ttgaagaaat ctcgatggcc
    19021 aggaagaatg gagcgtttga gtaacgagcc attagttgtt ttagatggtg cccataatac
    19081 gcatgcaatg aaccggcttg ttgagaattt gaaaaaagaa ttcaaaggat atcaaatcaa
    19141 cattttgttt tccgccttac gaactaaaaa tgttagagaa atgctagaac gattgcagca
    19201 agtaccaaat gtccatattt atttgacgac ctttgaacat ccaaaagcca tcgaattaag
    19261 tgaagtatca gatttagcaa gtgagagagt atcaatcgtt tcattgtggc aattcggatt
    19321 agcagatata ttggaaaaga tgacaaatga agacgtatta gttgtgaccg gttcacttta
    19381 tttcatttca gaagttcgcc atttattaat acaattagga ggagatcatg aagtataatg
    19441 cagtgatttt tgacatggat ggtttgctgt tcgacacaga gttagtgtat tacgaagcca
    19501 cacaagtagt agcagacaag atgggttttc cttatgataa agacctgtat ttaaaattct
    19561 taggtgtttc agatgaagaa gtatgggcga attatcatat gattttctcg gaatttggca
    19621 aaacaagtgt acaaagattc atagatgatt cataccagga aacgcttgaa cgtttttcgc
    19681 aaggagcggt acaaatgaag cccggtgtgt tggaactcct ctctttttta gaagaacatc
    19741 agattcccaa agttgtcgct tccagtaacc aacgagcagt cattgaactt ttattgaaga
    19801 agaacaatct gtttgaacga tttgagacga ttgtttctgc agaaaatgtt caacgagcaa
    19861 aaccagaccc agaaattttc ttgttagcta atcagaagct aggtacagcg aagaaagaaa
    19921 cattgatttt agaagattca caaaatggta ttttagcagc ttctgcggct gagatcccgg
    19981 tagtcatggt accagatctg cttccaccat cccaagaatt gatcaaaaaa acagctgggg
    20041 ttgtttcttc tttacatgaa atccctgcat tgttaaaata aaaatccatc tcttttcgta
    20101 tcttatttta gataagatag aaaggagatg tttttttggg aaaacgattg atcaaagaag
    20161 tacctactag ctctttacca agggaacgta tggaaattta tggcgcggaa gctttatcgg
    20221 atcaggagct tttggcaatc ttactgcgta ccggtcaaca cccttatagt gtgatgtcga
    20281 ttgctggaaa tctgttaaaa acgtttggag gactggcctc tttacgacag gctactttgc
    20341 atgagttaga agagattcga gggataggac gcgtgaaagc aatcgaaatc aaagccttga
    20401 tcgaattagg tcgacgaatt cagacggatc atgaaagagc ggctccaccg gttcgtacca
    20461 gctacgaatt ggcccagcaa ttgattctgg aaatgaaaga tcacaaacaa gaacatttgg
    20521 tttgtattta tttggacacg aagaatcaag tgatcttaaa aaaaacagtg ttcatcggtt
    20581 cgctgaatca gtcagtcgct catcctagag aaattttcca ttatgctgta cgttattgtg
    20641 cagcaaggat cgttttagcg cataatcatc cttctggtaa tgttattcca tcgcagcagg
    20701 atatgaattt taccaaaagg atacaaaagt gtggagaaat gatggggatc actgtactgg
    20761 accacttgat catcggacgc aaacgatatt ttagtttgag agaagaagga atgatggaag
    20821 agaagtaagt tgctttcttg caaaaaaaat gaagcaagtg tacaattata ttgtaatttt
    20881 gtttgggaat agaagttgaa acatctgttg cacctctatc gtacacgagc aatgttacaa
    20941 tttaaattag tgcttttggc acgaggagga tattattatg gaaaacggaa cagtaaaatg
    21001 gttcaacgct gaaaaaggat ttggatttat ctcacgtgaa gatggaagcg acgtattcgt
    21061 tcacttctca gctatccaag gtgacggatt caaaacatta gaagaaggtc aagcagtgac
    21121 tttcgacgtt gaagaatcag accgcggccc acaagctgca aacgttgtaa aagcttaatt
    21181 ttatctgtaa atcaaattga tggtagacat caatttttac gagagaacaa agcgagtaac
    21241 aaaaatacct gccacaagag acaatttctc ttgtggcagg tatttttttg tatgaaaaag
    21301 taaataaatt taagaggcta tgaaagagcc accttgtatc aaggaataag aacaaacaaa
    21361 aatggcttct catgtttttc atttgttttc tcttatattc aggacaaggt gctgatttca
    21421 taacctctta tttattcttc atctggtgtc attaccattg gcaaaatcat tggatgtctt
    21481 tctgtacgtt cgaataagaa aggctgaagc gcgccagtca tcgcttcttt caatttatat
    21541 tctgaacagt caggtccatt caaacattct cttaaagcgt ggaataagat tcgctgagct
    21601 tcattgatca tttctcctga ttctcgcatg tagacaaatc cacgagagag aatatctgga
    21661 ccagccaaga tttctttttt cttgatatcc actgtcgcga cagctaatac aagaccttct
    21721 tctgaaagca ggtgacggtc acgtaaaacg acattaccaa tatcaccgat cccgctacca
    21781 tcgatataca catctccagc cacaaaatgc ccagcaggac gcgcagaatc gctagttaac
    21841 gctaaaacat cgccattttc catgatgaag cagttttctg caggaacacc tacatcttgt
    21901 gctagagaag catgtacttt caacatgcgg aattctccgt ggacaggaat aaaatatttt
    21961 ggcttcatca aacgaagcat taatttttgt tcttcttgtc caccgtgacc ggaagtatgg
    22021 atgttattga tttttccgtg aatcacttct gcaccagctt cagacagcaa attgatcaaa
    22081 tgattgacac ttgttgtatt accaggaata ggtgaacttg aaaagatcac tgtatcgtct
    22141 ggctggattt ggatttgacg gtgagtacca ttcgcgatgc ggctcaacgc ggccatcgct
    22201 tctccttgag aacctgtaca taggatcatc gtttcattgg caggcaagcg attgatttcg
    22261 tgtgcatcta caaatgtgcc gtctggaact ttgatatatc ctaaacgttg accgttgacg
    22321 atggcatttt ccatgcttcg tccgaaaaca gcgattttac gaccagttgc cacagcggca
    22381 tctgctgctt gctgcaaacg gaagatgtta gaagcaaagc tggcaaaaat gatacgtcct
    22441 tcgatttttg aaaagatctt caaaatagaa tgcccaatcg ttttttctga tttagtaaat
    22501 gttggaatct cagcatttgt actatcagat aaaaggcaca agactccttc ttccccgata
    22561 cgagccattt tatgcaagtt tgctggttcg ccaacaggag tgaaatcgaa tttgaagtct
    22621 ccagtcgcta cgatatttcc agaaggtgtt ttcaccacga tccctaatgc atctgggata
    22681 ctatgggtcg ttctgaaaaa gctgacagaa gtctttctaa aacggatgat cgtatcttca
    22741 ttgatctcat ggagctctgc atctcgaagc aagccatgct catctaattt gtttgtgatc
    22801 aacgctaatg cgagaggacc agcatagata ggaatatttg cttgtttcag caagtagggt
    22861 attccgccga tgtggtcttc gtgcccgtga gtaatcacca aggcttttac tttgtgtaag
    22921 ttttgtatga tgtagctgta gtcgggaatg acatagtcaa ttccaagtaa atcgtcttcc
    22981 gggaatttga tcccggcatc aatcaaaatg atttcatctt ggaattgtac cccatacgta
    23041 tttttcccga tttcacctaa tccaccaata gcaaagacgc ctgtctcgtt atttttgatg
    23101 gaaagtttca tattaaaact ccactaatga gaaatcagca ttttcttttt cgtactctag
    23161 atgacttcct tctagaagtt gaacgaattc aatattgtat ggggtatttt cttccacacg
    23221 ttggcgagcg tctacatcgc tttttgcctc aatgtaaaga gattttgttt cttcccgttt
    23281 agggttgcgt acttttgttt cttgataata aactttatag atcattgttt tgtctccttt
    23341 atctgcgtaa agcagtgatt tagctagttg atttttttag acaacacaaa acgaggggac
    23401 aagccacctg gtttttcata tgctttgact agaatactgg attcgagtca aagagacaag
    23461 cctcgttttt gcttatctgt tcggatattt tttcaaccgt accttctgta taaaaaatat
    23521 acaacaatta attgttattc tagcataaaa cagcaaataa gtatagaaat caactagatt
    23581 gaaagaaaaa atcaatgttt atcatcccgc tataatcata atgacttttt tatgagagaa
    23641 gtgttctatt aataggctcg ttgtgctatc atttttagta atgaaaatgt aacaaaagat
    23701 taaaaggaga tgagaaagtg aaactaatgt ggcattatac catgcgttat aagaaattct
    23761 tgtttttcaa tttcatatgc gtatttggtt ttattttgat tgaattagga cttcctacga
    23821 ttttagcacg tatgatcgat gtaggaatcc gcaatgatga ttttgattat gtgaagcaac
    23881 aagggatatt tatgatcgtt attactgtaa tcggtattat tttgaatatc ttgttaggtt
    23941 attttggttc gaggattacg actaatatcg tagcagatat tcgagatgat ttgttcgaaa
    24001 agatccaaag ttattcgcat caggaatacg aaaatctagg cgtatcttca ttgatcactc
    24061 gtacaacaaa cgatgcttat cagatcatgt tgttcctgca aaatatttta cgtatcggtt
    24121 ttatggcacc aatgatgttc attgtcagtt tgtacatggt catgcgtaca agtgtgacac
    24181 tcagtttgta tgtagtaggt gcattacctt tactattatt ggcagtcgta gcgattgcta
    24241 aggtgtctga accgttatct aaaaagcaac aaaaaaactt agataaaatc aacagtatct
    24301 tgcgggaaaa tctttcagga ttgcgtgtga ttcgtgcatt cgtcaatgaa aagtttgaag
    24361 aaaaacgttt tgctgtagtc aatgagaatt atgcttctag ttcaaaaagc ttattccgtt
    24421 tgatggctgt tgcacaaccg ggtttttatt tcttatttaa tatcgtaatg gtattgatca
    24481 tctggagcgg ggcagtgcaa atcgatgcag gcgagcttcg tgtaggagat ttgattgcgt
    24541 ttatcgagta tatattccat gccttgttct cattcatgtt atttgctagt gtgttcatga
    24601 tgtatccaag agcggctgtt tcagctggac gtatccaaga agccctagat gccaaacctt
    24661 cgattgtaga agatccaaat ggtatatctg atacaaaaac taaagggtat atcgaatttc
    24721 aaaatgtaac atttgcttat ccaggccatt cgcaagagcc agtgatacga aatgtcagtt
    24781 ttacagcctc tccaggagag accgttgctt ttattggaag taccggtagc ggaaaatcta
    24841 cgttgatcca attgatccct cgcttttatg atgtgagtga aggaaagatc ttgatcgatg
    24901 gagtagacgt gagagattat cggctaagca agttgcgaga taagattggg tttattccgc
    24961 aaaaagcgct gctatttact ggaacgattg cagataattt gcgatacggg aaagaagatg
    25021 ctacgcaaga agaaatggaa cgtgcagctg agattgctca agcttcagaa ttcatttctc
    25081 gaaaaccaga tggatttaat gaacatttat cagaaggtgg ttccaatttt tcaggtggac
    25141 aaaaacaacg attggcaata gccagagcca tcatacgccg tccggaaatc tatatttttg
    25201 atgacagctt ttctgcatta gattatcaaa cggatgcgaa gttacgtgct cgtctgaaaa
    25261 aagaaacgac agaatctact gtattgatcg tagcacaacg tgtgggaacg atcatgcatg
    25321 cagataagat catcgtatta aatgaaggag aagtcgtcgg tatggggacg catcgggagt
    25381 tgttggaaaa atgcccgatt tattatgata ttgccgcttc tcaattatca aaggaggaat
    25441 tagcatgaaa aaaacctttt cttccttaaa acgtttagct cgatatatca gaccgtatcg
    25501 tgagactttt cttttagtga tcctatttac agttctgaca gtggctttca acgtggcttt
    25561 gccatatgtt acaggacttc ctactacaga aatcagccgt aatattgcca atggcgaatc
    25621 aatcaatttt tcttatatat tcaaatgtct catttggatc acagttgttg gaatagggta
    25681 ttgtatttcg caattacttt ctggtgtttt gatgacaaat gtcgttcaaa gtgccatgca
    25741 tgatttgaga agagacatag aagaaaaaat caatcgtctt ccggtttcat atttcgataa
    25801 aaaccagcaa ggaaatattt tgtctcgtgt gacgaatgat gtagatgcag tcagcggtgc
    25861 tttacaacaa gcttttattg gtatcgtaaa tgcgatattg ggaattacgt tggctgcagc
    25921 aatgatgttt tatatccagc cgatgatggc gctgatctct atgatcatga ttccaacatc
    25981 gatctggatc tcaaaacgag tgatcaatgc ttcgcagaaa tattttcaaa gcatgcagaa
    26041 ttctcttggt gagctaaacg gctacgtcca agaaaatatg acaggtttta gtgtattgaa
    26101 agtatatgga cgcgaaaaag aaacattgga aggattcaaa aaagttaatc acagcttgaa
    26161 atactttggg tttcgtgcag cattcatttc tggcttgatg atgcctctag ttcaattaac
    26221 tgcctatgct acttatattg gtatggctgt tctaggaagc ttttatgtta ttactggggc
    26281 gatcgttgtc ggtcaactac aagcattcat ccaatatatt tggcaaatca gtcagccaat
    26341 ggggaacgtg acacaattgt cttcagtttt gcaaagtgca tcagcggcaa cgaaacgtgt
    26401 attcgagatt ttagatgagc cagaagaaaa agaaaacaaa gtagatgctt cactgccaga
    26461 aacgatttta ggagatgtga cattcgatca tgtcgatttt gcatatgatc cgaagaaacc
    26521 attaatccaa gatttgaatt ttgaagtgaa agctggtcaa accgtagctg tggtcggtcc
    26581 gaccggtgcc ggcaagacga cattgatcaa tctattgatg cgtttttatg acgtagacaa
    26641 aggagcaata aggattgatg gaatcgacac caaatcaatg agtcgcagtg atgtgcgttc
    26701 gctatttggt atggtcttgc aagatgcatg gctttatcaa ggaacgattg cagataatat
    26761 ccgcttcgga aagcttgatg cgacagatta tgaagtagtt gatgctgcga aaacagcaaa
    26821 cgtagatcat tttatccgaa cgatgccaga tggatatgat atggccatca attctgaggg
    26881 agacaatgtg tctttaggac aaaaacagtt gctgacgatt gcccgagcag tggtctccga
    26941 tcctaaaatc ttgattttag atgaagcaac aagttcagta gacacccgtt tagaagcgtt
    27001 gattcaaaaa gcaatggatc gcgtgatgga gggacggaca agtttcgtca tcgctcaccg
    27061 tttatcgaca atccgtgatg cagatctgat tctagtgatg gatcaaggtc agatcattga
    27121 aaaaggaacg catgagagtt tattggcaca aggtggattt tatgaaaaac tgtataacag
    27181 ccaatttgct gaggaagcag aataagtcat ataagaataa aacagtttaa aacttatttt
    27241 ttgttctcta ttcttgataa tggtaaagta aagttatcaa gaaaagtgag gacacggaga
    27301 tggaacagtt tttaaatgaa aaaattactg aagccaaaag agaagttgat cgtttaacgc
    27361 acgtctgtca gacgaagcct tttgattcag ctgatttttt tgaaaatgag tttcaaatcg
    27421 aacgcttggc agcacaaata gaagtgtacg aattaatttt aaaaaaagta atagaagaaa
    27481 atatagatgt acaataaaaa caggtaaaac gtcagttacc agatactgta aatagacatc
    27541 tggcctgagc aggttttacc tgtttttttt cttatgaaat aaatatatgc tatgatacct
    27601 ttattcaata ggattagttt ctacaaaaaa tgaaaggaga aaaataaata tggaacggtt
    27661 tatagcatca aatgccacag tgattggtga tgtgacattg tcagaagatg tgacgatctg
    27721 gtatcaagct gttttacgcg gggacagtaa ttggataaaa ataggacaaa gaacgaatat
    27781 ccaagatggt acaatcatcc atgtcgatca tgatgcacct gtagatattg cggagaatgt
    27841 gacggtagga catcagtgca tgctacatgg ctgtacaatc gaaaaagggg cgctgatcgg
    27901 gatggggacc acgatattga atcatgcggt tattggtgag aatagtttga ttggtgctgg
    27961 atcattagta acagaaggaa aagttattcc tcctaatgtc ttggcttttg gtcgcccagc
    28021 taaagtgatc cgtcctttga caaaagaaga aatacaaaaa aacaaagaaa acatccagca
    28081 ttatgtcgag ataggtcgga tgcatgcgca aggaaaattc aaggagcaaa gataggcagt
    28141 agcatccata aaataaaaaa ggatggtcag tttgagttga ccatcctttt ttatttggtt
    28201 tagataagag agcttaaaaa agcttccttt gaaacagatt aatttaatga agaactgttt
    28261 tccatgactt catcgatcaa tccatatgct tttgcttgtt cagctgtcat atagttgtca
    28321 cgatctgtat ctttttcaat cacttcaagc ggttgtccag tgcgttcagc taaaattttg
    28381 tttaagcgtt cgcgtgtttg caaaatatgg cgtgccgcaa tttcgatttc agttgcttgc
    28441 ccttgagcgc cgccaagagg ttgatggatc atgatctcag catttggtaa agcaaatcgt
    28501 ttaccttttg taccagctgt cagcaagaag cttcccattg aagcagccat acccataaca
    28561 attgtttgga catctgcttt gacaaagttc attgtgtcgt aaatagccat acctgctgta
    28621 actgaaccac caggagaatt gatataaaga tagatatctt tttcagaatc ttgagcgtct
    28681 aagaaaagca attgggcgat gatcgaatta gcaagatcat ctgttacttg gccgcttaac
    28741 atgatgatac ggtctttcaa taaacgtgag tagatatcgt aagctctttc acctcgtgaa
    28801 gattgttcaa tgactgtagg aattaaattc ataatagagt tcctccttga tttcgtttag
    28861 aaatagtgta ccataattta gtttctaata cctaaaatgt agtatacatt tttggtcaaa
    28921 aaaggtcaaa taaaaaactt aggcaaatta gtcgcataaa cccgctttat ggtatgataa
    28981 gcagagaaac aaaacaagga ggaacaacag attgatagtt ggtattactg gccttatcgg
    29041 agtgctgaca ggtgctgtag ttgtctatat cggcatgata ctgcaaatgc gtcacttgga
    29101 aaataaagaa cagcgtagac gtgaattgga attgaaagtc aaagaaatcg agacgctgaa
    29161 tcaattaaat aaaaagatca acgagatctt gcaaaaacgg acaacgctga tggaagaata
    29221 tgtcagcttt gatgcatttg atgattgtta tatcacgatt gacgattttg cgtatctcca
    29281 atcattttca gcgcaaaata gtttttatct gccaaattat attttagacg agctatttaa
    29341 gaatatctcc catcgaaaag tgattttatc tccagatgaa acaatgaaga ttggtggata
    29401 tgcttataaa ggcggacgcg tgattttaga gaattttacg gatagtctga ctgaaatgat
    29461 caatgaaaaa aaagctcagt tgaagactat gacgcgccag ccgattggtt tcttctcgaa
    29521 agatgatgtt tggagctgat attttgaagc attgccgttg cacatgagag cgtggtaatg
    29581 ctttcttttt ttcttaatga tacaatagaa ctgaacggag tgaagaaaga tgttacaaat
    29641 ctatgcccat cgcggaagca gcggcaccca cccagaaaat acgcttcctg catttgcaga
    29701 agctgtacga gtaggtgcag atggtatcga actagatgtc catctaagca aagatggtta
    29761 tttgatcgtt atgcacgacg aagaagtgga ccggacgact aacggtaaag ggctgattcg
    29821 agaaaaaaca ttggaagaac tcaaaaaatt gaatgcaggg ggcaaattca gcaaagaatt
    29881 tccagcagct aaagttcctg aattgagaga agtgttagga ctgctcctcc aaaaaaatta
    29941 ccgagggatg ctaatgattg aaattaaaac tgatcagtat gaatatcccg gtatcgaaga
    30001 aaaattagcc gaaacattaa aaagtagaga ttggcctttt caacatgcat attgcagctt
    30061 tcatttggat tctttggaaa aaatcgcatt actggaaccg gagatacagc tggacttatt
    30121 gatgagtact tctgataaaa agatccaact agcagcagaa accccttata tagaaggagt
    30181 acatccaaaa aatacttggt tgttcgaaca tccagtccag gcagctgatt ttccaaaagc
    30241 aattcgtgca tggacgatta acgatgaaga agaaatggcc aaatgtcaag aactcaaaat
    30301 agatggaata ataactgatt ttccggagaa agctcttcgg tggaaacaat caacgaaaag
    30361 taggtaaaat atggaaaaag tattagtgat cgtcggtccg acagcagtcg gtaaaacagc
    30421 gctaagtgtg gaactcgcac aactatttgg aggagagatc atcagtggtg attcattgca
    30481 aatctataaa aatctggata tcggtacagc aaaaatcaaa gaagaagaga tgaataatgt
    30541 tccgcatcat ttgattgatg tgatcgaacc tactcaacgt tactcagccg ctgattttca
    30601 gcattcaggt cgacaattga ttaccgagat tgcgaaccga gggcacctac ctatcatagc
    30661 tggtggtacg ggtctctata tccaatccct tctatatgat tatcagttag gagcgacaga
    30721 agaagcgaat cctgagatta gaaaaaagta tgaaacgtta gctgaaacaa ttggttccca
    30781 agcattatgg aatcagttga aagaaaaaga cccggcagct gctgagaaaa tccactggaa
    30841 caatcaacgc aaagtgataa gagctctgga agttttagag acgacaggac atagtatcac
    30901 ggcacctaaa gaagagccaa agcaattgta tgattacctc atgattggat tagatacgaa
    30961 gcgtagtatt ctttatgaac gaatcaatca aagagtcgat ttgatgctag cagaaggttt
    31021 agtagcagaa gcgcgtaagg tgtatgagct tggagaagtc caggccagtc aaggtattgg
    31081 atataaagaa tttttccctt attttgaagg gaaagaaaca ttggaagaag caacagaaca
    31141 agtcaaacta cattcgagaa gatacgcgaa acgtcagttg acgtggtttc gtaaccgttt
    31201 ggatgcacag tggtttgatc ttataagtga gccaaatcaa aaaagtcaga ttgagcaaac
    31261 aatcaaaaaa tggctggagg cggaataaaa tggaagaaaa agtgatcatc gtaggggtag
    31321 aaaccgaagc aaaccagcgt tacttttcag agtcaatgga agagctagtt cagctgacga
    31381 ataccgcatc aggagaagtc gtattcacaa ttacacaaaa gagaccacaa gtggatcggc
    31441 aaacgattat cggtaaaggg aaattacaag aattagttca acaagcggat gcgcatgaag
    31501 cggatttgat catctttaat catgagctaa ctccgagaca gagccaactg attaccgatg
    31561 ctgttggtct tccggtaatt gatcgcgtgc agcttatctt agatattttt gcgatgcgtg
    31621 ctcgttcgaa agaagggaag ctgcaggttg aacttgctca gttagaatac ctgctgcctc
    31681 gtttagtagg gcaagggaaa acattatctc gtcttggcgg tgggatcgga acaagagggc
    31741 caggtgaaac aaagttagaa acagaccgcc gacatatacg gaataaaatt tttgctgtta
    31801 agaaagaatt gaaagaagta gaagctcatc gagagcgcaa tcgccaaaaa agaaaaaata
    31861 gcgagatttt tcagattgga cttattggct acacgaatgc cgggaagtca actatcttga
    31921 atctattgac acaagcggat acttattcga aagatcaatt gtttgcaacg ttagatccat
    31981 tgactaagag atggcgtttt gctgaaggat ttgagattac tgtaacggat accgtcgggt
    32041 tcatccaaga cctgcctacg caactgatcg atgctttcca ttctacatta gaagaaagtc
    32101 aaaatatgga tcttttgcta catgtagtcg atgctagttc tccagatcgt attttacagg
    32161 agcagactgt cttaaaattg atggatgaac tgaatatgaa agaaatgccg atactgactg
    32221 tttacaataa ggcagaccag atcgacccag cgatgttcac gcctacgcta ttcccaaatg
    32281 tattgatttc tactcagacg aaagaaggta agatggcgtt gatcgaagca atcaaacgtc
    32341 agctgatgga actaatgacc ccttatacac tttctgtgcc aagcaacgaa ggacagaaat
    32401 taagcgagtt gagaagacaa acaatggttt tggacgaagc gtacatggaa gaaacaaacg
    32461 aatatgaagt taaagggttt gcaaaaacca attcaaaatg gttaagaaaa aaccaataaa
    32521 attttatttt tttaa
//
LOCUS       DACDIH010000027        30407 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDIH010000027 DACDIH010000000
VERSION     DACDIH010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.027"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..794
                     /locus_tag="IV281_001324"
                     /pseudo
     CDS             1..794
                     /locus_tag="IV281_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
     gene            934..1860
                     /gene="cysK"
                     /locus_tag="IV281_001325"
     CDS             934..1860
                     /gene="cysK"
                     /locus_tag="IV281_001325"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP8780344.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2096..2542
                     /locus_tag="IV281_001326"
     CDS             2096..2542
                     /locus_tag="IV281_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8780345.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2814..4166
                     /locus_tag="IV281_001327"
     CDS             2814..4166
                     /locus_tag="IV281_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8780346.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSDPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQEALGEIETAQ
                     "
     gene            4337..4624
                     /locus_tag="IV281_001328"
     CDS             4337..4624
                     /locus_tag="IV281_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780347.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            4850..5293
                     /locus_tag="IV281_001329"
     CDS             4850..5293
                     /locus_tag="IV281_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8780348.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5299..6483)
                     /locus_tag="IV281_001330"
     CDS             complement(5299..6483)
                     /locus_tag="IV281_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP8780349.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6751..7449
                     /locus_tag="IV281_001331"
     CDS             6751..7449
                     /locus_tag="IV281_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP8780350.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7456..8133
                     /gene="nth"
                     /locus_tag="IV281_001332"
     CDS             7456..8133
                     /gene="nth"
                     /locus_tag="IV281_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP8780351.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8259..8792)
                     /locus_tag="IV281_001333"
     CDS             complement(8259..8792)
                     /locus_tag="IV281_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780352.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9089..11470)
                     /locus_tag="IV281_001334"
     CDS             complement(9089..11470)
                     /locus_tag="IV281_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8780353.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11460..12107)
                     /gene="recU"
                     /locus_tag="IV281_001335"
     CDS             complement(11460..12107)
                     /gene="recU"
                     /locus_tag="IV281_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP8780354.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12171..12710
                     /locus_tag="IV281_001336"
     CDS             12171..12710
                     /locus_tag="IV281_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP8780355.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12800..13219
                     /gene="gpsB"
                     /locus_tag="IV281_001337"
     CDS             12800..13219
                     /gene="gpsB"
                     /locus_tag="IV281_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP8780356.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13241..13608
                     /gene="rnpB"
                     /locus_tag="IV281_001338"
     ncRNA           13241..13608
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IV281_001338"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13817..14983
                     /locus_tag="IV281_001339"
     CDS             13817..14983
                     /locus_tag="IV281_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP8780357.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15029..16525
                     /locus_tag="IV281_001340"
     CDS             15029..16525
                     /locus_tag="IV281_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP8780358.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     LLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16606..17289
                     /locus_tag="IV281_001341"
     CDS             16606..17289
                     /locus_tag="IV281_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8780359.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            17934..19124
                     /locus_tag="IV281_001342"
     CDS             17934..19124
                     /locus_tag="IV281_001342"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP8780360.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19226..20179
                     /locus_tag="IV281_001343"
     CDS             19226..20179
                     /locus_tag="IV281_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP8780361.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20248..21597
                     /locus_tag="IV281_001344"
     CDS             20248..21597
                     /locus_tag="IV281_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8780362.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21780..22103)
                     /locus_tag="IV281_001345"
     CDS             complement(21780..22103)
                     /locus_tag="IV281_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780363.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22232..23395
                     /locus_tag="IV281_001346"
     CDS             22232..23395
                     /locus_tag="IV281_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780364.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23465..24814
                     /locus_tag="IV281_001347"
     CDS             23465..24814
                     /locus_tag="IV281_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8780365.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24859..25290)
                     /locus_tag="IV281_001348"
     CDS             complement(24859..25290)
                     /locus_tag="IV281_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8780366.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25678..28320
                     /gene="alaS"
                     /locus_tag="IV281_001349"
     CDS             25678..28320
                     /gene="alaS"
                     /locus_tag="IV281_001349"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP8780367.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28520..29116
                     /locus_tag="IV281_001350"
     CDS             28520..29116
                     /locus_tag="IV281_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8780368.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29238..30386
                     /locus_tag="IV281_001351"
     CDS             29238..30386
                     /locus_tag="IV281_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8780369.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
BASE COUNT        10158 a         5249 c         6344 g         8656 t
ORIGIN      
        1 ggaacgagtc ttttagttgg gccgatgatg gttacagcgg taccgcaaca tgttcatgca
       61 gatgaacaag gatctcaaac agttgctgat cctcaagcgt ttatcgatca aatcggctgg
      121 tctgcgtcag aagttgctgc aagtaatgac ttgtatgctt cagtcatgat cgctcaagcg
      181 ttgttggaaa gtgcatatgg cacttccgga ttagctagtg cgccaaatta taatctattc
      241 ggtgtgaaag gaagctataa cggacaggtc gtttatatgg caaccaaaga atatgtaacg
      301 gacaatgggt aacgatgaat gaaccattcc gaaaatattc ttcttattgg gaatccttcc
      361 aagaccatgc gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg
      421 gcgtgtggaa aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt
      481 atgctaccga tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa
      541 cccgttttga tacgccatca acaggttcgg ctaccacaag tacaacaaca acgactacta
      601 cgacgacttc aacgtcatca tcttcaacaa cagatgtaaa tacaaataat ggtcagacat
      661 atacagtcgt gtcaggtgat acgctatggg atattgcaca aaaatttggc atgtctgttg
      721 atgatctaat ggcgaaaaat ggcatgtcta tgtccagtta ttctttatta gttggtcaag
      781 aactaaacgt ttgatttaga taaaaaggca aagaaactac catatatggt atatctttgt
      841 cttttttttg ctagtcaaag acgaatcact cgccataaat gaacagattg tattatgata
      901 agtaagaaga gcaaagacaa ggaggaattg aagatgacag ctatctataa ctcagtcacc
      961 gaattgattg gcaaaacgcc aatcgtcaaa ttaaataaaa tcgtaccaga agattcagca
     1021 gatgtttttg taaagcttga gttttttaat ccaggaggaa gtgtaaaaga tcgtattgct
     1081 ttaagtatga tagaaaaagc agaacacgat ggtctactga aaccaggtga tactatcatt
     1141 gaacctactt ctggaaatac aggaatcgga ttgtcgatgg taggagttgc aaaaggatat
     1201 aaagtaatca tcgtgatgcc tgagacgatg agtatcgaaa gacgtttatt gatgaaaggg
     1261 tacggcgcag agttgatttt gactcctggt gcggatggta tttctggatc aatcagagaa
     1321 gcagaacgtt tggcaaaaga aaacggctac tttttaccat tacaatttga aaatgaagca
     1381 aatcctcttg tgcatgagaa gacgactggt cctgagatac atcaagcatt tggagtaaat
     1441 ggcttggacg cgtttgttgc aggtatcggt acaggaggaa cgatcactgg cgctggccga
     1501 gaattaaaac gtgtgtatcc gaaaatcgaa ttaatcgggg tagaaccagc agaatctgct
     1561 attttagaag ggaaagaagc gggacctcac aaaatccaag gaatcggtac aggttttgtt
     1621 cctaaaacac tggatacttc tgtgtatgat aaagtccttt ctattagtgg agacgaagcg
     1681 atggaaacag ctagagaagt tgggcgaaaa gaagggattt tagtaggcat ctcatctggc
     1741 gctgcaattg ctgccgcgct aaaagtcgca aaagaattag gaaaaggcaa gaaggtatta
     1801 gctgtggttc ccgacaatgg tgaacgttat ttatctacag ccctttacca agagatataa
     1861 ggcacttggt ttttctccag ataggtttat gtaaaaaact tttaaagatg aaaagaacac
     1921 acaaaaattt atgatgagaa aaattatttg aatagagacg taaaatagtg aacgcgactg
     1981 ttttacgtct tttttcatta aaatacaata aaattgaatt ttcttttcat gttaagtgga
     2041 ttttaaaatg taaaaatagt atgataagac gagaataaca acgtggggtg tttacatgga
     2101 aaataagtta gtcgaagagg ttatcgaaaa gttaaaaaaa gagaacattc gaatcacacc
     2161 tcaacggtac gcggttttag aatatttgat tgaacatcat tcccatccaa cggctgatga
     2221 gatttataaa gcacttgaac atcggtttcc aaatatgagt gttgcgactg tttacaataa
     2281 ccttcgctta tttacttcga ttggatttgt tcaagaaatg aagtacggcg atgcttctag
     2341 ccgatttgat ttcagttcaa agcgtcatta tcatgtaatt tgtgaaaact gcggtaagat
     2401 tgttgatttt cattacccag ggcttgaaga tatagaaatg gctgcgagca gactgactga
     2461 ttttgagatt aatgaacatc ggttggaatt gtatggactc tgtccggatt gtaaagagtc
     2521 agcaaaaaag gcgcaaatgt aaaaacagtt tctataagtg ttatacctaa aaaaagctag
     2581 ctgtgtaaaa aaacgctagc ttttttattt ttttgtcttt tattcacaat cttagatatc
     2641 aaagtgaata ttatcacaat aagaatcttg tagatattgg gttcttatgc ttgaaaagga
     2701 atgaaactac aaacaaacct ttgtgaattc acaagcatta aactgttgta tccgttttat
     2761 ttctgttata gtagttttgt cgaaggacaa aatgttaatc ggagggacca taaatgaaag
     2821 tagtagtaat tggctgtact catgcaggta ctgcagctgt aaaaagtatt ttaaaaaatc
     2881 atccagaagc agaagtgaca gtatatgaaa gaaatgacaa tatttctttc ttatcttgcg
     2941 gtatcgcact ttatgtcggc ggagtagtaa aggacccagc aggcttgttc tactcaaatc
     3001 ctgaagaatt atcttcatta ggagcaacag ttaaaatgga acacgatgta acagacgtcg
     3061 atacagaaaa taaaacagta acggcaaaag atctgaaaac aggagaaaca gaaacggttt
     3121 cttatgataa attagtgatg acaactggtt catggcctat tatcccaccg attagtggaa
     3181 tcgatgcgaa aaatgtactc ctatgcaaaa actacaatca agcaaatgaa attattgcac
     3241 aagcgaaaga agcaaaacgt gtagtaattg ttggtggtgg atatatcggt attgaattag
     3301 tagaagcttt tgtcgaatct ggaaaacaag tgacattgat tgatggatta gatcgcatct
     3361 tgaataaata tctagataaa ccattcacag atatcttaga aaaagaattg acagaccgcg
     3421 gtgttactct tgcgctaggc gaaaatgtta ctgaatttat cacagatgaa gaaggaaaag
     3481 tgaaacaagt agctacacca acagatacat ttgatacaga tatggtcatt ttatgtgtgg
     3541 gcttccgtcc aaatacaaaa ctgatcgaag gaaaagtaga tacactgcca aacggggcaa
     3601 tcaaagtgaa tgaatacatg cagacaagtg atccagacat ttttgcagct ggagactctg
     3661 cagtagtcaa ctacaatcca agtggcacgc aaaactatat tcctttggca acaaatgccg
     3721 ttcgtcaagg tttacttgtg ggaaataact tgacagatca taaaatggcg tatcgtggta
     3781 ctcaaggaac ttcaggactt tacctatttg gctggacgat cggttcgaca ggtgtgacca
     3841 aagaaagcgc aactttgaac gatctagaag tccaagcgac tgtatttgaa gataattatc
     3901 gtccagagtt catgccaact actgaaaaag taatgatgga gctagtctat gaaaaaggaa
     3961 cgaatcgtat cgtaggtgct cagttcatgt ccaaatacga cattacacaa tcagcaaata
     4021 ccatgtcttt agctgttcaa aataaaatga ctgtagaaga tctcgcactt tcagatttct
     4081 tcttccaacc acattttgac cgcccatgga attacttgaa cctacttgca caagaagcgc
     4141 taggcgaaat cgaaactgca caataaagaa aaagggttgt gacacaattt tgtcacgacc
     4201 ctctgtcttt ttttgtgaaa agtacaatta gttgtctttc ttcgaatttt ttgtcacaat
     4261 aagcagcaaa gataaagctg ttacataaaa gaacaagcag ctttatttga ttttgaaaag
     4321 aaaggtgaga aagaagatga ataaagaata ttttgtccaa caagatgcat ttttagaaga
     4381 ttataaaagt gcttcgaaga aattaacgat caatattgat gagttggcag atcaagtact
     4441 ctctcagttg aatgaaacgc aagaggaata ttacacgatg ccagcaaatg acgccaaaga
     4501 taatgtttct catcgttttc ctttcagtag acgcatctac tcagatgaaa atgacggcac
     4561 attgaataca gagtacacat atgaaggtca gccatatgtg ctagaaactg atgatgatct
     4621 ttaaagacag tttttgaaaa ggccgctcat ccttaagtga tgacaaaaaa tcatcacaat
     4681 taccgatgac tgtctcttta ctatctttta atcagaaaaa ggggttatga caagctttgt
     4741 caaccccttt ttttcttggt ttcagattgg gttttcctac aaaatatttt tatctttgtt
     4801 gaataggaaa caaaaaaaca atataatgaa agaaaaagaa ggtgtagaaa tggataaaag
     4861 acaactgcta atagaagaat atctggattt agctaatgag attattcatc gtaataaacc
     4921 agaaatggaa aaagaatttg caggatttac tctaaatgaa ctagaagtga ttgaacatat
     4981 cgggaaaatc cctgatccta acgtgacaaa attagcgaat gctagttaca tgactagagg
     5041 agccatcagt aagctaacaa aaaaactgat agctagaggg atcataaaca cttatcaaag
     5101 cgaagagaat aaaaaagaaa tctattttca attgacgcca atcggacaaa aaataaggaa
     5161 tagacacgag caactccatc aaaaatgggt acagcgagat gctactgtat ttgaaaatat
     5221 gtcaaaagaa gaatttgaca cagtatttcg ttttatcggt cgatacagag aatttatacg
     5281 aagtagcaaa tgaagctact attttttatt gtagtgataa taggcaaaaa tcataagcaa
     5341 aaatccgaat accgtcggaa gaaatggtgc tgcagcataa agctgtcctg cgattagcgg
     5401 gccgatgacg cgcgatagag attgtatact ttgggtaccg cccattacaa gaccttgctg
     5461 gttatctgag acagaattgg atagttggcc attgaataca ggagtaaaaa tagaatcacc
     5521 gaaaccataa cagataatcc caaataaaaa caaaatcggc agtagaagaa ttccagataa
     5581 tgtgatgaat agataggcga ggatttcact gaacatccct attcttgtga tctgctgttc
     5641 tgaaaatttt ttgagcaatc gaggcataat gaacaattgc gagacagtat ccaagattcc
     5701 aatcaaagag aagcttaaac caaccaaccc agccttccac tgaaaggcgt caatcgaaaa
     5761 ttgagaaaag atagattgaa gagagccagc tgccaaccaa acagcaaaac ctgctgtcaa
     5821 taaaggaata acggatgaaa tcttgaataa ttgttgtaat tggtgaaaag gtcttacatg
     5881 ggagaaactt aaatctactg aacgtttttt tattggaagg ctttctagca taaatgtgta
     5941 gccataaacg gcatttaaga aagtaaagag tgctccaata aatataggaa cagaattccc
     6001 caactcagct aatagacccc ctataatagg acccagcgtt gttcctatac cgactaaagc
     6061 acccatccat ccaaaaactt tggttctttc attaggttcc gtgatgtcgg caaagtaagc
     6121 atataaggtg gagatttctc ctgccgtcaa tccttcaatg atacgaccaa ggaacaacat
     6181 ccatatcgaa tttcctaaac caaaaagcaa atatccgata cttgatccta ataaactgat
     6241 aataaggatc ggatgacgcc caaaatgatc acttaagcta ccgagaatgg gggcagataa
     6301 gaaggcagct agagcataaa cagacatgag caaagtgata tagaaagctt gctgatgtat
     6361 ggagtgagaa taaggcagcg ttaaaaacgg aagtacagga ctgacgatag taaatcctaa
     6421 tccagtgaat aagacagaga cgtatccaaa aactttagaa tgtttatttg tgaaactgta
     6481 catgtaaaaa ctcctttatg caataatgtt aacttggaaa caaaaatagt gtatagtaat
     6541 tttgtttcct tgtcaacatt ctaaggaaca gcgaaatcta cttaatttat ttattccgaa
     6601 aagtgaagat acgcatcaaa aacatgaaca aacaaaaatc aagtagacga cattgtaaat
     6661 ctgaccgatc agtgaagtaa atagaaaaga aacctgttct tgcttcccat gtttttttta
     6721 cgtatactgt gattgagcgg aggagaaatc atgttgaatt tagaaaatta cttaagcgct
     6781 ggacaaacag tcatatctaa tcttttactt aaaaattatc ataggatcgg tctacaatct
     6841 gaagaatttt tatttgttct acagttgcat atggcacagc ttgaaggaga tactttccct
     6901 gatttgcaga tgattgctat ggatatggga atcaaacaag atcagatttt tcaaatactg
     6961 gatcgtctcg taacaaatgg atttatcaag atagaaacga cgattgtcaa cggtaaaaaa
     7021 gcagatcgat ataatcttta ccccatctat gatctattag gtgagtattt aaaaacacaa
     7081 gaaaaaaaat acgaagaaaa aacacaagaa aaagaaatcc agtcagtgta tcaattattt
     7141 gaacaagaat ttggacgccc gctttcttcg atcgagttcc agcggatcgg acaatggcta
     7201 gaagaagacc attatcagcc ggaaattttg aagctagcac tgcgggaagc cgtattaaat
     7261 caggcttata gttttaatta cgttgaccgc attttgctat cgtgggaaag aaaaaatcta
     7321 cgtacaaaac aacaagtgga agaagaccaa aaacgaagaa aacagcagct tcttcaaaga
     7381 gaagtagaac agtcagctca acaagaagaa ttgccgaaag tctcactgaa aaattggttg
     7441 gaggagtagc ggaatatgtt aagcaaacaa aaaacaatgg aagctctaga aacgatgtat
     7501 ggaatgtttc ccgaagcaca tggagaactc aagcacaata atccatttga gttattgatt
     7561 gcagtgatct taagtgccca ggcgacagat gtatcagtga ataaagcaac gccagatttg
     7621 tttgcctctt ttccgacacc cgatgcgtta gcagaagctt caatcgatga aatcattctt
     7681 aaaataaaaa cgattggttt gtatcgaaat aaagcaaaaa acatcaaagc gtgtgcacag
     7741 cagctgattg aacgtttcga tgggcaagtg ccaacgtcaa gagaagaatt gatgtctctt
     7801 cctggtgtag gcagaaagac agcaaatgtc gtattggggg atgcattcgg gataccagca
     7861 atcgcagtag atacccacgt ggaacgtgtc tcaaaacgcc tgaggatctg caaactagat
     7921 gctacggtca tggaagtaga agaaacactg atgcggaaag taccacaaga attatgggta
     7981 aagacacatc atacactgat tttttttggt agatatcatt gcacggcaag aaatcccaag
     8041 tgcgaggtat gtcctttgct atcaatatgt caagatggga aaaatcggat gcgtctaaaa
     8101 gaaaaggcgc taaaaaagaa accacgcctg tagacgtggt ttctttaatt gcaaataaat
     8161 gtatgaatta tcttgttatt atggagaaaa gctgtttcgt ttatcttttt tgcatgaaaa
     8221 agtaaatgta catactcagt agctaggatg gtaacttatt acaatttatt gcgataaaaa
     8281 taaaagttgt tatttttatt gggaaaatac ccgatgtaga aaataatgcc ttccaatata
     8341 cctataaaat aagtccagta ttccaaagga ttatataaat aagtgacgta tctatctaaa
     8401 ggtgtaataa aaatgataag tgataataaa aaaataccat ttataactaa acataaacta
     8461 atggatttgg gcggtttcag gctcatcaga aaaatataga ttctggtgaa caacaaccat
     8521 aaaaaagtga gaatcattgc atgagagctt ctttccataa attttccttt aagagtagtg
     8581 cttaaaagta cagaaaaaat aataaatgca aggataggag caattaaagt aaatttattt
     8641 aataataaac aaaatttttc agctttttta ttcttggaat aacgaggtat tgttttcgat
     8701 aaaaaaagat ggtgcagaca aattatgata attaagcata ctatcagata actcaaaatg
     8761 aagccttctt tcaaaaaatt attcaaaacc atatattgta ttttttattt gataataaca
     8821 atgggattat taagaagtgg cactaaataa aagactagtg tgtatttagc cgatttaatt
     8881 ataaacaagt aaatggataa agtatatgta atgataaaag aaaaatggat tttctaattt
     8941 ttaagctaaa tgttagatca cacatgtata tgctggatag tcttataaaa aatcatgtaa
     9001 tacagcttgg tacatgattt ttatttgtag ctgtttttta catagacaca aaaaagaaac
     9061 cacgcttgta gacgtggttt cttttttact atctgtttcc agaagctgag gtgctgctgg
     9121 atggtgtatg gtcatttgca gcatccgctc cgctagttgc tggctcactg gaagaagcag
     9181 gaggagcaga tgaagacggt gtcgagcttt cttcggatga agaagaagga accgttgttg
     9241 aagacgctgg ctcgcttgtt tctgctgtcg tgctttcttt tgaggactca gcagttgatt
     9301 cgctttgact agtagagcta gagctttgag tcgttgtctc tgttgttgag cttccaggag
     9361 tctggacata gctggaagat ggaatagttg ttgatggtgt aattgcattc gatctagctg
     9421 tatattgatc tttgtagtac agttcaccgc ccacacgaat taatccgctt ggcatctccc
     9481 agtctgtatt tgtcacgtta gctgaaacat actgcataag ctcgcgataa acatcagagg
     9541 ctacgtgtga cgattctgaa gtgacaggtg tcattttttt attgtaacct gtccacacgg
     9601 aaatcgaata atttggcgta tagcccgcaa ataggatatc tggatacacc ccagaagaaa
     9661 taccaagttt tgcatactcg tcatctgtgt aattggatgt tccggtctta ccggcttgat
     9721 ataacccagc gatttgcgca ttcgtaccag ttccttcagt gatcgtatct tttaaaatgt
     9781 ctgtgatcat ataagctgtt tctggagaca tggctgtttt tccatctggc tcataagttt
     9841 cttctgttcc gtcttggaag acgattttat taacatattg aggtttgtaa taagtccctc
     9901 cattggcgaa agccgcatag gctgctgcca tttttaagga agaagctccg tattttgtcc
     9961 catcttgttc ttccgtatta ctagagatcg cattcgattg atggatcgta ctgtattcga
    10021 tcccaagatt ttttaagaaa gaagccactt tatctgaacc gacttcattg aataatttga
    10081 ctgctggaac attacgggat aagtaaagtg cttggcgtaa agtaatggtg cccatatatt
    10141 gattatccca gtttcctaca ggagtcgaag tcccttcata gttatacggt gcgtcagtaa
    10201 tagtttttcc agtagaatat ttcaaatatt caaaagctgg tccgtaatct gtgacaggtt
    10261 tcatcgtaga accaaaatca cgggaagtat tcactgctaa gttgttgcct aaagttacgt
    10321 cttctgcgat gtgacgtccg ccaatctggg cttttacttt cccggtattg gtgtcgataa
    10381 gggtggaggc aacttgcatc tcgtcgtctg ggtaagagac atactgatcc gtgttgacga
    10441 tatcatatag tttcttttgg gcgtccagat ccaaattcgt ataaatctct aaaccgtctg
    10501 tgtagacatt cttatctgtt ttttcttgta cttcattgat gacttctttg acataattat
    10561 cgacgatctt agtattgtca tcggattgag ttagttcttg cagaccatcc gttaccggta
    10621 cattaacagc ttgatcatat tctgtttgag agattttttc gttttgaagc atcgtataca
    10681 aaacagtatc tcgtcttttc ttcgcctgat ctggataaac ataaggatca taggcggaag
    10741 gggcttgagg catcccagca agcaatgctg tttgcggtaa agaaagttct gataatttct
    10801 taccaaagta catctcagaa gctgtctcca tcccgtacaa gccgtttgac atatacactt
    10861 tatttacgta gtaggtcaag atctcttgtt tcgatttttt ttgttccaat cttacagcca
    10921 tccacgcttc ttgggccttg cgtttcagag tctgatcctc ggcactggtg gagaagaagg
    10981 aaagtttgat caattgctgt gtcaaagtac ttccgccttg cagtccgcca gaggtaaagt
    11041 tagagagggc agaaccgatg atccgaatag ggtcaacccc gatgtgttta taaaaacggc
    11101 gatcttctac cgacacgatc gcatcttcta atgttttcgg taattcattc gcagaaattt
    11161 tttcacgctt ttctgcgcct aaatcttcga atagttcgcc atcttgtgta tatagtttgg
    11221 aagatacggt agcgtctaat tttttatccg tcaattcggg cgcatctttt gcgtaatacc
    11281 agaaaagtcc tacacctgct aaaaagagga tacagaaaaa tgagagtatt cctaaaagga
    11341 tcttgataaa taatcctttt ttatgtgttc cagaggactt tccagaacct ttgccagaat
    11401 tcttcttcac actcttttta ggagtaggct gtttctgtcg tctagagctt ctagtttgtt
    11461 catttgccat tcttgctttc tccttcaata gataatgttg ataaatagaa gtcaaccgcg
    11521 tccaaataag gaatccgcgg agaaatacca gttttgatct ctattcctag ttcttcgatc
    11581 aaagataaag gtagtgattt gcgtcctttt tccttttggg cgtcccagta agcaatcaaa
    11641 tgaacactat tcaaataaaa acagcgcttc aaagacgaaa accataacaa gacaaagcag
    11701 accccatttt gttcgaggca tgctttcata tgttcgattt gatgggcatg gaagttttta
    11761 aagggaaaag atgttttgtt cttggtttct tttgcttcaa agtccaagta aaagcctcga
    11821 tagacgccat tatagtctgt agttgaggct tgggtaaagt aggcttcctt gatcacagca
    11881 gcacttctct taggatagtc tactttcacg atttggatag gggtaggctt tttatggata
    11941 accgccaacc cgcgggaaag ataatattta ttactttcat tgattgcttc ttcaaagcgc
    12001 atcccccgat tgccgaattc agtgatctga tttctttttt cttgaatttt ttcgacggga
    12061 gaggcattct tataataagg ttggccattt ggatagcgca ataccatctt taatcacact
    12121 cctaggccac attatagcaa aaaagcggat agaaagaaaa ggaatctgag atggataagt
    12181 caaaagtaat ctatgtgtct gggtatcgaa gtttcgaact cggtgttttt aaagaaaatg
    12241 atcctaaaat ccaagttgtt aaaaatgttt taaaaaaaga actccatcag ttagcagaag
    12301 aagggctgga atgggtactt gtcagtggga atttgggcgt cgaactatgg gctgtgcaaa
    12361 tagtagcgga attgaaagaa gaatatccgg aactatctct tggtattttg tatccttttt
    12421 ctgattttgg taataactgg aatgaaggga accaagaaaa aaaacaatta gcagaacaat
    12481 tagcagatta tgtcgaagct gttagccatc aaccctacca atcacctagt cagctaaaga
    12541 atcacactcg ttttatactt gagcatacag tgggctgtct gctgatctat gatgaagaat
    12601 tccctggaaa aacgaagtat ttttttgaag atgcacagaa ttaccaagca gatcatccct
    12661 acgaaattcg gctgatcagt atggacgatc ttcaaaattt tagtgaataa tcgatattgt
    12721 gtttgattta tcaacttttt tcctatacaa tgaaaaaggt agaaatgtta ttacgtaaat
    12781 gaaaactaga ggtgtaaata tggcgaattt ggtttatagt cctaaagaca ttttacaaca
    12841 agaattcaaa acaaaaatgc gcggctatga tcccgttgaa gttgatgaat tcttagacaa
    12901 tatcatcaaa gattatgaaa cttacagcaa agaactcctt gctttacaag aagaaaacga
    12961 tcgattgagt gcaaaagtag ctcaattatc taaaactcaa ggagcagctc aaacacgcgt
    13021 gcaacaaaca gaggcaccaa agagcgcagc agtgaccaat tttgatatct taaaacgctt
    13081 gtcaaatctt gaacgtgaag tatttggtaa aaagcttgat caacaagcaa gtgcagtcaa
    13141 accagcacaa cctaatccaa ataactacac aaatgcagac acaagcttag atgataatga
    13201 aaaaacacgt caattttaat ttttgattaa tgaatttgcg gttttcgggt aatcgcggtc
    13261 tgacttgatc aggctgagga aagtccatgc tcgcacaggc tgtgatgcct gtagtgttcg
    13321 tgcttagcga aaccataagc taaggtactc tttggagtaa cggcaggaaa acaagctaag
    13381 gcttcggcta tgcttaagta tccttgaaag tgccacagtg acgaagcggc tggggaaacc
    13441 catccggtgg aacgcggtaa acccctcgag cgagcaaccc aaacaatggt aggggcgctt
    13501 tttgtctgga agaagaacag gacaaaaagg cagtataact gcagatagat ggttaccgct
    13561 ttgttttttc tccctggaaa agcaaagtac agaacatggc ttatagaaaa ccgcaaacat
    13621 cagggaatta ggtcgtgaca aaagtaatgc gttataagaa acaagaaaaa catttgaaag
    13681 ctatttttca atacttcaga tgaatcgttc ttattacaaa acaaactttt gtatcaccgg
    13741 ttattcggaa tttgagtaaa agtgtggggt aggactgatg tcccacctcc tttttttata
    13801 aataaggaga ttgtgtatgg aaaaaaacag gaagttcaac ctcgtcgcaa ctgcggcaag
    13861 cgggctagaa gcactggttg gtaaagaatt aagagatctt ggaatcgaat gccaagttga
    13921 gaacggacgt gcacgttttg aaggaacaat ggaaacgatc gctacagcta atttatggtt
    13981 acgtacagca gatcgagtaa aaatcgtggt tggggaattc gatgcgtata cgtttgatga
    14041 attgtttgaa aacgtaaaag cattgccttg ggaagatttt cttccactag atgcagcttt
    14101 tcctgtagct ggaaaatcga tcaagtcgaa actatatagc acacctgatt gccaagcgat
    14161 cacaaaaaaa gcaatcgttg aacgtctccg tacctattat caccgtccag cttctgtacc
    14221 actgacggaa actggcgcac attttcagtt ggaagtagct ttgctgaaag accatgtgat
    14281 ggtaactctg gatacgacag gaccaagttt attcaaacgt ggttatcgct tagaaaaagg
    14341 tggagcacca ttgaaagaaa acatggcagc agcacttatt atgttgacaa attggcgaaa
    14401 agaccgtcca ttttatgatc ctgtatgtgg ttcaggtacg ctttgtattg aagcagcctt
    14461 aataggccat aatatcgcac caggattcaa tcgtgagttt gcttgcgaat cttgggattg
    14521 gttcagtcaa gaaatcatgg aaaatgttcg atcagcagcc gaagaaaaag cagattatga
    14581 tattgaatta gatattactg gttcagatat caatggacgg atgattgaaa ttgccaaagc
    14641 caatgcagaa gaaatcggat taggctcttc gattaccttt aagcaacaag cagtcaaaga
    14701 cttcaaaacg gataaagaat acggggtcat cgtggctaat ccgccttacg gagaacgtct
    14761 aggagaagaa gaaactgttc ggaaattata taaagaaatg ggcgatgtat tccgtccatt
    14821 aaaaacatgg agtaaatata tcttgactag cgacttagcg tttgaggaat tttacggaca
    14881 aaaagcaacc aaaaaaagaa aattgtacaa tggtgcattg cgtacggatc tattccagta
    14941 ttggggaact cgtccgccta gaaaaaaaga gaaagacgaa taatcttact aattgattca
    15001 ttcaattgga tgtttggagg aatagggaat gaaagaaaaa gagtttttac aagaagtaaa
    15061 agaaatcaat cttctacaac aagctttagg aatcctcgat tgggatacac aaacaggtat
    15121 gcctgaaaaa gctagtgcat accgaagtga agtagacagc tatctttaca gcatttattt
    15181 ttcaaaaaaa atcggtccaa aaatcaaaga agccattcgc tatttttctg aacatccgga
    15241 agaattgtcg gaagttggaa ctgccgtttt tgccaaagta aaagaagagt atgatttgga
    15301 acatcgcgta ccggaagatc ttatgagaaa atatacagct gccgtttctt cagcacacgg
    15361 acaatggcaa aaagctagag aaacacaaca attttctgat tttcaacacg cattgaatca
    15421 aaatattgaa ttaacaaaac agttgatacc ttactggaga aaagatgaaa agacgaatta
    15481 tgatgttttg ctgaatcaat acgaacccgg gatgactgtc gaaatccttg ataaggtatt
    15541 tgatcagctt aaagaaggaa ttctttcgat ccgccgtacg ctccaagaaa aagggactga
    15601 acctgaaaca gactttttaa atcggagaat gacgaaaaaa caacagaaga gatttgtagt
    15661 gaaagtaatc gaacaacttg gttatgattt tagtcgtggt cgtttagatg acactgtcca
    15721 cccatttatg actggaatta atccgaatga tgcacgaatc acgacacgat ggaacgaaac
    15781 agatttcaaa atggctgttt ttggagtgat ccacgaggct ggtcatggta tgtatgaaca
    15841 agacatcgat gataaatatg cgtatacacc ggtttatgaa ggtacatcaa tgggtatcca
    15901 tgaatcacaa tcgttgttta acgagatcat tattggaagt agtcgcagct tctggaaaaa
    15961 gcaatatcct ttctttcaag aatgtgcaga aggaacattc gatgatattc cttttgacaa
    16021 attttatcgt ttattgaacc atacaaaatc aagtttggtt cgtatcgagg cagacagttt
    16081 gacttaccct ctccatatca tcattcgtta tgaaatcgaa aaaatgattt tcaatgaagg
    16141 ggtagatgtt gctgaactac ctgaaatatg gaatcaaaaa tatgaagagt atctgggaat
    16201 acgaccaaca aatgatgtgg aaggcatcct acaagacgtt cattggtcag gtgcaagctt
    16261 tggttatttt ccctcttatg cattagggta catgtatgcg gcacaattac ttcattcttt
    16321 gcagcaagaa atagatgtgg aaaatgttct agcgtcagac gattattctc caatcaaaaa
    16381 ctggatgtca gagcatatcc atcaatatgg tgcatctaga aaaccaaatc aattaatcat
    16441 ggatgcaaca catgaaccgc ttaatccgca atacctgatc gactatatga aagacctgta
    16501 ttttaatgta tatcaggtaa aataaaatac aatttacaaa gatagctgaa attcatctaa
    16561 tctatagatg ttttcagcta tttacttaag aaagaagtgg aacgaatgga taaattacaa
    16621 gtgatcgctg attatagtat ggaccaatta gcgcatgatc aaacaggaca tgggagcgat
    16681 cataccaaaa gagttgtcaa gttagctgaa aaaattttgg agacggaacc gcaggcagac
    16741 cgctttgtga cgcttgcagc agcttaccta catgatacga ttgatgataa agtcgttaaa
    16801 aatgagaacg aggcaaagca acagcttcgt gtattccttc gctctttgcc aatagtagag
    16861 gaacagatct cgatgatttt ttccattatc gaaaatatgt ccttttctaa aaatctctct
    16921 gaagcggccg aactatctct ggaaggtaag atcgttcaag acgctgatcg tatcgaagca
    16981 ttaggagcaa tcggcatttt gcgtactgct tatttcggcg gtggacatgg gcacccgatt
    17041 tttgatcctg aactgtatcc gcaaacattc aaggacaaag aaaattaccg aaaaggcacg
    17101 acagtgatca atcattttta tgaaaaactg ttcttgctgt ctgataaaat gaatactgat
    17161 tacggatatg aagaagccaa acgaagagaa accttcatgc gtgaattctt agaagaattt
    17221 tttctggaat ggtctattga cgatgaaaat tttactccag acaattggat caaaaataac
    17281 aaaaaatgat ggaggttgtg acagaagtgg tcagctccaa gatatttcgg atcattttcc
    17341 gtaaaagtta cttctggaac actgtttacc aagaggtcgt gagcctgccg ttcagctccg
    17401 agtcacaagg aacaatttca aaaaatagct tctcgtgttt gatgaatatt gtaacttgtg
    17461 attcggaatt ggcaggagaa caccgtttat tcggaagaat aggactgtga caagactttt
    17521 gtcacagtcc ttttttcgaa ttcaacaagt aaaactttta agaaatattc agagagtaaa
    17581 attaaaaatt gctgataaac gaagatggaa attttatgta tttttattgg tctatgcaat
    17641 aaataaatta aatattcaga atacaattga ttttaacgaa agggcttcta tcaatggttg
    17701 tgttgtattt atctgcccag gtagagtaaa atagatcatt atctaaaagg taattgttca
    17761 tactatataa gtcatttgaa agtaaaaaga tactgtaaat aaaaaatgtg gttaaataat
    17821 tctcataatc agtgtaagcg ttatcaaaaa tatagcatac ttgctttgta gatgaatgac
    17881 acgatatatg tttggtatgt tctgcaagac agatgaatat ggaggtggag acaatgtctt
    17941 ccaaagaact aacaatgtat ttagagaaag aattggatca actgaaagaa aaggggttat
    18001 acaatacgat cgacatacta gaaagtgaaa atggtgcttg tatcatcgta gacggaaaaa
    18061 agatgatcaa tctagcatcc aacaattatc ttggatttgc caatcgcgaa gaattgaaaa
    18121 aagcatgtat tgaagcaacc gaaacttatg gtgtaggagc aggagcagtc cgaacgatca
    18181 atggttcgct gaaaatccat cagcaattag aagaaaaaat cgcagaattc aaagggacag
    18241 aagcagcaat cgcctttcaa tctggattca actgcaatat gggcgctatc tctgcagtaa
    18301 tgacaaaaga agatgctatc ttatcggatg aattaaacca tgcatctatt attgacggtt
    18361 gccgcttatc cggagcaaaa atcatccgaa ttaaacatca agatatgaaa gaccttgaga
    18421 aaaaagcaaa ggaagcgata gaaagtaaga aatataaaaa aatcatgtat atcacagatg
    18481 gtgttttctc catggatgga gacattgcac gattgccgga aattattccg attgttgaaa
    18541 aatatggact gatcacctac gtcgatgatg cgcacggctc aggcgtaact ggaaaaggtg
    18601 caggaaccgt caagcatttt ggattaagcg acaaaatcga tatgcaaatg ggcacgttat
    18661 cgaaagctat aggtgttgtt gggggttatg tagctggatc gaaaacacta atcgactggt
    18721 tgaaagctag aagtcggcca ttcctatttt ctacttcgct aactccaggt gcagctgcag
    18781 ctgcattggc ttcgattact ttgatgcaag aacatccgga acttgtcgaa aaagtttggg
    18841 aaaatgctaa ttattttaaa gaagaattga aaaaagtagg ctataacatc ggtatgtctg
    18901 aaacacctat tactccagtc attttgggag acgaaaaagt gactcagact ttctctaaga
    18961 agctgatcga acatgggatt tatgctaaac caatcgttta tccaactgtg cctcttggga
    19021 caggcaggat cagaaatatg ccgactgcgg aacatacgag ggaaatgctt gatgaagcag
    19081 tagcagttta tcaaaaaata ggaaaagaaa tggaaattat ttaactaaat ttagtttttt
    19141 cgtctattta aaattagaga acatgttcac gcatatattg ttaatcatct gaagatagta
    19201 gtaaaaaaga tcagggagga tcatcatgaa aaaagtattg gtgacaggat gtttaggaca
    19261 aattggttca gagttgatca cacggctacg tcaagaacta ggcagtgaaa atgtcattgc
    19321 gacagatata cgcatgcctg aagtcattga gaaaaacgat atctttgaga tcctagatgt
    19381 aatggattac gaaaaaatgc gcagcatagc agaacaatat caagtggaca cattgattca
    19441 tttggctgct cttttgtcag ctgttgcaga agcaaaacca caattagcct ggaatttgaa
    19501 cgtgactggg ctggtcaatg cattggaagt cgctagagaa ttccagctga aattttttac
    19561 gccaagttca atcggcgcgt ttggtccaaa tactccaaaa gatcataccc cacaagatac
    19621 tcttcagcgt cctacaacca tgtatggggt aacgaaggta gctggagaac tactatgtga
    19681 ttattattat gtgaaatatg gcgtagatac tcgtggtgtc cgtttccctg gattgatttc
    19741 ttatgcaacc ttgccaggag gcggtacgac agactacgca gtcgatattt attattcagc
    19801 aatcaaagaa ggaaagtaca ctagtttcat tgatcaaggt acatcaatgg atatgatgta
    19861 tatgccagat gcgatcgatg ccattataaa actcatgaat gcagatcctg atcgattggt
    19921 tcatcgaaat gccttcaata tatcggcgat gtcttttgac ccggaagaga tcaaagcatc
    19981 tattcaaaaa atcataccag attttgaaat ggattatgat gtggatcctg tacgtcagtc
    20041 aatagcagaa tcttggccaa attcactaga tgtttcttgt gcgcaagagg aatggggctt
    20101 ttcgccgaaa tacgacttag atgtgatgac aaaggatatg ttggaaaaac tgaaagaaaa
    20161 attggtagag acggtataaa acaagaacca catccaaaaa gttttttata cttttgggat
    20221 tggctatggt atgaaagggg acaagggatg gaagaatatc aagccacgcc agtaaaagaa
    20281 gtaaaaatag aaaatgaatt aaaacgtacg atgggatttt ttactgcttt atcgactgtt
    20341 atgggcacag tgatcggtgc aggggtattt tttaaagccg caagcgtagc agaagttaca
    20401 ggatccgcca gtttgcatat gttttcttgg tttctcggtg gtatgatttc tgtctgtgca
    20461 ggactaacag gagctgaact ggcagctgct attccagaaa caggagggat gattaaatat
    20521 atcgaacgga tttacggaaa tacagcagct tttttattgg gctgggcgca agtcgtgatt
    20581 tattttccag caaatgtagc agccttgtcg atcattttcg gtacacagtt cgtcaatcta
    20641 tttggtcttt cccagtcaat gattgttccc gtagctgtta cagctgctgt atcgatcctg
    20701 ttgatcaatt ttttaggttc taaagctgga ggggcttttc agtcgattac gcttgtctgc
    20761 aagcttattc ctttgtttgt gatcgtcatt ttcggtttgt tccgacaaga aggagtagac
    20821 ttccaactgt ttccgatcca agcaggagaa aatctttcat ttttctcagc cttgggagcg
    20881 ggcttgctag caacgatgtt cgcatacgac ggttggatac acgtaggaaa tatttcggga
    20941 gaattgaaaa agccggcgaa agatttgcca aaagcaattt ctttaggaat catcggaatc
    21001 atgatcgttt atttattagt aaatgcggtg ttcttaagaa ccgcatctat cgacggagtc
    21061 gcaggaaaca gcaatgcggc aagtgacgtt gcaaaaatga tttttggcgg cttcggtgga
    21121 agactggtca cagttggtat cttgatttct gtttatggaa ccatcaatgg ctataccctt
    21181 actggaatgc gtctccctta tgttatggca aaagaaaaca acctgccttt tagcaagctc
    21241 tttgctaaac ttcatgataa aactaaagtt cctgtagccg caggtatctt agagctagtc
    21301 atcgctattg gcatgatgat gatcggtggt tttgatacac taacggatat gctgatcttt
    21361 gtcatttgga ttttttatac gatggtcttt gttggtgtca ttcttcttcg taaaaaagaa
    21421 cctgatttgt ttcgaccata caaagttcca atgtatccgt ttattccact agtagcaatc
    21481 attggaggga cttttatcgt atcaagcacg cttatcactc agaccttttt agcttcgatg
    21541 gggatagtat tgacacttgc tggtatcccg atttatctct atttaaaacg tagatagcta
    21601 aaacattcga attttattag aactgcactt ctctgccaaa caaaatattt tatttaaatg
    21661 aggagtacgt ggaagaaatg tcaaaagtac tcgttaattt tgatcacgta aatgtcgttt
    21721 tgtctacgat gaaatagcaa agtaaaagac agaaatcggg ctcaaccgat ttctgtcttt
    21781 tactttgatt tgcgtaactg aactattcgg cgtacgccgc gataaatatt taataaaacc
    21841 aagcaaccag aaatgaaaat gggaagccaa atcagataga cagggtagcc aagcacttta
    21901 gacaaagcat gtggagtcat tttatccatt tcaagaagtg cttgctgcca agtctgaaga
    21961 ttgaagacta gcatgaaacc cgaaataatc aggagaataa atcctaaata atgaattgta
    22021 gttctttttc ttacataagc gatcgtagca taaaggaaca gtaaaaaaat aggtacgcca
    22081 aaaacaaagt acatattcag catctcgaca ccccctttgt gtttatctta ctttactttg
    22141 aaagaaatga gaaataatag cctcgttact tgcttgaaac agcaaaggac tgctaagata
    22201 agagagatac aaagaatcaa ggaggagtca tatggcaagt tatatcgaac cgattcgttt
    22261 tgcgctgatt gtttttccat ttttagcctt agcgatctca tctgtttttt ttatttatga
    22321 atatcgaaag tatggaacat ttctagtaac aagggcagtt attttgtatt ctttcgtttt
    22381 ttatttattg tgtgcgtatt ttttggtgat cttaccactt ccggcaaagg agacggttgc
    22441 ccaaatgaca gggccaaaga tggagctgca tttatttgct tcatggcaac atttcagaga
    22501 caacacggta ttggtcttgt ctgaacctag tacctacctg ccggcaatga agcagagtgt
    22561 atttttggaa ccagctttta atgttttatt actcatacca tttggcattt atttaagata
    22621 ttatttccgg ctttctttta tgaaaacgat gattgctagt ttctgccttt ctctattttt
    22681 tgaattgacg cagctaagtg gactttattt tatctaccca agaccatacc gcttatttga
    22741 tgtgaatgat ttagtgacaa atacattggg tggaatcatt ggttttgtcg ttacgccgtt
    22801 atttacattt atgcttccta caagaagacg aatggatgaa ctttcttatg aaaaaggaaa
    22861 gactgtctca ttgactcgac gcttggtcgc ttatttgatc gactggacat ttttatctct
    22921 tattaatttg gtagcggcga ttttctttaa aattgcgttc gattcagggg cagttacttc
    22981 tcatatctgg tggcttttat tagaagtagg ggtatacttt atgattgtac cgtttataac
    23041 caatggatgg acacttggca aaaaaattgt gcgtatccgt ttggtggaag aagaccgcga
    23101 acgaatttct ttaaaagcat tgatgatccg ctacgggtat ctgtactttc ttttttatgg
    23161 tttaagctgg ttggggcagt tgcttcagca aatcatgaat tcgacaacga atacggtgtt
    23221 gcagatgagt gcagtattca tttctctcgg tgtcttgtta ctacaaggta tttttatttt
    23281 aaatatcttg ctatcatttt tgagaaaaaa acggattctg ttttatgaaa aagctagtca
    23341 cacatatact gttagcacga ttggccaaac aaaggatgat gattctcgaa attagctgag
    23401 caaaaacgct ttgtctgtgt tataatgcca aagtcgtgat aatttaaaga gaagaggtgc
    23461 acgaatgaat aattttgata tattccgctt caaaccgttt atcaatgaag cgttaaccgc
    23521 aaagggcttc aaacagccca cagaggtcca ggaacgtttg attcctttaa ttgcaaaagg
    23581 aaaaaatatc attgggcaat ctcaaactgg tacaggaaaa acacatacgt ttttgttgcc
    23641 tttgtttgaa aaaatcaatg cagaaaaaga ggaagtacaa gttgttatta cagctccaag
    23701 tagggaacta gctgcacaga tctatgaagc agctacacaa atcgctaaat tttctacacc
    23761 ggagatccgt gtctcgaatt ttgtcggcgg tacagacaaa cagcgtcaat taaatcgtct
    23821 gcatcaccaa cagccacagg ttgtagtagg cacgcctggt agaatattag acatgatgaa
    23881 tgaacaagcg ctgaagattc acaccgcatt tgcatttgtt gtcgatgaag cggatatgac
    23941 attggatatg ggttttttaa atgaagtaga ccaaattgca agtcgattac cagaaaagct
    24001 gcagttttta gttttttctg ccactattcc agaaaaactt cgtccattct tacgaaaata
    24061 catggaaaat ccaatcattg aagaaatcaa accaaaagca gttatttctg aagacattga
    24121 aaattggctg atttcaacaa aaggtaaaga tgagaatcaa atgatttatc aactgctgac
    24181 aattggtcat ccttatttag caatcgtatt tgccaataca aaacagcatg tggatgaaat
    24241 tgccgattac ttgaaaaacc aaggcttgaa agtagcaaag attcatggag acatcacccc
    24301 aagagagaga aaacgagtga tgcgacaagt acagaatctg gagtatcaat atgttgtagc
    24361 gactgattta gctgctagag ggatcgatat cgaaggtgtc tctcatgtta ttaatgcaga
    24421 aattccagaa gatctagact tctttatcca tcgtgtcgga cgtactggac gtaatggctt
    24481 aaaaggaacg gccattacgt tatattcgcc aaatgatgag caagccattg atgcgataga
    24541 aaaattgggc gtctcatttg aaccaaaaga aatcaaaaac ggggaaatcg tcaaaacata
    24601 cgatcgtaat agacgtgcca aacgagaaaa atcaaaagat gtattagatc ccacgctgat
    24661 tggtcttgtg aagaagaaaa agaaaaagat caagcctggt tataagaaaa aaatcgattg
    24721 ggcaattgca gaaaaaaata aaaaagaacg caaaatcgaa cgcagacagc aaagccgtac
    24781 tgctcgtaaa aataagaaaa attcacataa ataaaaaaca agaggttgtg acaaaaggcg
    24841 tgtcacaatc tcttgttttt aagctagact ttttttcatt ccgatacaaa gtacttcatc
    24901 ttcaataaaa ggttctgtgc aagtatgata cccttgtttt tcataaaaat ctacagccgt
    24961 catttcagct actacataac tagtctgaca accttctgaa accgcccgtt tttcagcagc
    25021 ttcaagcaac tgaccgccta acccttttcc tcggtattct tttgctacgc aaaatcgttc
    25081 cggcttgaag caagtaggtg ttttcttttc ataacgcaaa gtagctactg gtcttttttg
    25141 atcaagaaat agataatagg tttgttgcgg gatatctttt ccgtcgaatt ctagttctgc
    25201 aggaatccct tgttctaata caaaaacttg atatctcaaa aagcagctag ctgcgcgtat
    25261 ccattcatcc gctccaacta tttttttcat catattcctc cttgtcatga atagcagaga
    25321 tgtattgatt cctttattct tatcgtacca aaaatcgtgg agtatgacca aaaggaaatg
    25381 aaaataaaaa ttgagatatt gacagtgatc aatagaatgc gtatactaat taagtacgaa
    25441 aaggatatga tttgtttttt aagactcaca gaaaattctt tcttggctga aaaaagaata
    25501 gtcactaatc aaattgctcc ctttttctat tttggtagaa ataccgacgc agctcagcgt
    25561 tatcgagttt aagaggtaat gattacacat cattgcaatc aaggtggtac cgcgagtttt
    25621 cgtccttggc tgtgatggtg tttttttgta tttaatttat agaaagcagg taatgacatg
    25681 aaacaattaa gtagtgcaca agttcgacaa atgtttcttg atttcttcca atctaaagga
    25741 catacgattg aacctagtgc gtctttgatc cccgtgaacg atccaacctt attgtggatc
    25801 aactctggtg ttgcaacatt gaaaaaatat tttgatggtt cagttgtgcc agaaaatcca
    25861 agaatcacaa atgcccaaaa atctattcga acaaatgata tcgaaaatgt cggtaaaaca
    25921 gcacggcacc atacgatgtt tgagatgctg ggtaattttt cgattggtga ctatttcaaa
    25981 aatgaagcca tccactgggc atgggaattt ttgactgatc caaaatggat ggcattcgat
    26041 ccagaaaaac tttatgtaac agtttatcca aaagatacag aagcaaaacg catctggcat
    26101 gaagaagttg gcctgccact agatcacatt attgatgtag aagacaactt ctgggatatc
    26161 ggtgcaggcc catgtggtcc ggatacggaa attttctatg accgtggaga agcttataac
    26221 gatttagcag aagacgatcc tgaaaactat ccaggaggag aaaatgaacg ttatttagaa
    26281 atctggaact tagtattttc agaattcaac cataagcctg ataacacatt tgaaccactt
    26341 cctcataaaa acattgatac ggggatggga ttggaacgca tggtctcaat cgttcaagat
    26401 gcgtcaacga actttgagac agacttgttt atgcctatta tccacgccgt agaaaagcta
    26461 agcaaacaag taaaatatgg tgaaaaccca gtaacagata tttcttacaa agtaatcgca
    26521 gatcatattc gcgccctttc atttgctatt ggcgacggtg cattgccatc gaatgaaggg
    26581 agagggtatg tcttacgccg tttgcttcgt cgtgctgtca tgcatgggaa aaaattaggt
    26641 attgaacaag cattcttata tcgtttagtc cctgttgttg gggaaatcat ggtaagttat
    26701 tatcctgaag tacttcaaca aaaagaattt attgaaaaag ttatccgtac agaagaagaa
    26761 cgtttcaatg aaacaatcaa tgaaggtttg gaaatcttaa atcaagtgat ccaagaagta
    26821 aaaaacgaaa acgggacgac tctagcagga aaagacatct tcaaactata cgacacatat
    26881 ggcttcccgg tagaattaac ggaagaggtg gcagaagatg aaggactgaa agttgatcat
    26941 gctggttttg aaaaagaaat ggatgcacaa cgcgaacgtg ctcgctctgc ccgcagtact
    27001 gaaaaatcaa tgggggtaca atctgcttta ttgacagata tcaaagtaga aagtaaattc
    27061 attggataca cagaacaaga atcagatagt caattattgg tcatcgttaa aggtgagtct
    27121 cttttgaatg aagtcgcaga aggtgaagct caaatgatct ttgatcaaac acctttctat
    27181 gcagaaatgg gtgggcaagt agcggatcaa ggatggatca tgaatgaagt aggcgaaatc
    27241 gtagcagttg ttacagatgt tcaaaaagcg cctaatggtc aatttatgca caccgtagaa
    27301 gtaaaagcac caatgagtga aggacaaacc tatcatctat tgatcgatca atcgatgcgc
    27361 aatcggatca tcaaaaacca tacagcaacc catctactgc acaaggcatt gaaagaagta
    27421 cttggcgagc atgcaaatca agctggttct ttagtcgctc ctggacattt acgattcgac
    27481 tttacccact ttggtcaaat cacttcagat gaattagctg aaatggaaag aatcgtcaac
    27541 gaaaaaatct gggaagctat cccagttgaa acaattgaaa cagatatcga tacagccaaa
    27601 aacatgggtg caatggcttt gtttggtgaa aaatacggac atgacgttcg agtagtaaat
    27661 attggcggct ggtcaatcga actgtgcgga ggcacacatg tagcaaatac agaaaacatc
    27721 ggaatcttca aaatcgtttc tgaatcagga attggtgcag gcgtgcgacg aatcgaggct
    27781 gtaacaagta aagaagcgta cgaactactt gaagaagaag aaaaacaact aaaacaagta
    27841 gcaacaattg ttaaatcacc tcaattgaaa gaagttgttt caaaagcgga acaattgcaa
    27901 cagcaagtaa gagagctaca aaaggaaaat gaacaattag ctagcaagtt agcgaatcaa
    27961 caagctggtg atgtattcaa agatccagaa acgatcaatg gaaaaacaat cattgcagca
    28021 caagtaaaag tgaaagatat gaatcagctg cgtcaattag ctgaccaatg gaaacaaaaa
    28081 aatatttcag atattcttgt tttagcaaac gaacaagaag gaaaagtcag tctgctagcg
    28141 gcaatgacga aagaatcaaa tgaagctgga ctaaaagctg gcgacttgat taaagcaatc
    28201 gcaccgaaag taggcggtgg cggtggtgga cgtccagaca tggcacaagc tggcgggaaa
    28261 aatcctgctg gtattccaga tgcacttaaa gctgcgaaag aatgggttgc gcaacaatag
    28321 ggcgtttacg tttgacagaa aaacaaaagt tcaaacattg aacgaaacat gattatgggg
    28381 gcgaagacaa acaagtcttg ccctcatact tttttcaatt ggatggcgca tctaatcaaa
    28441 tggtcggatt cggatggtct ttagttgacg aaagtgacag aaatttttac gattagtata
    28501 aataaaaagg ggtgaaaaaa tgaatagtag aaataagacc tttcgactag tcttacgtgc
    28561 gatcttgtta gcaatcatca tcgtacaagc gatggttcct tggctggggt ttattccatt
    28621 aggattcatt agtttaacaa ttatccatat cacagtaatt attgctgcag tagtattagg
    28681 cccaaaagat ggcatggtga ttggcttgtt ttggggaatc gctacaattg ttcgtgccta
    28741 tgccatgcca acaacgccat ttgatacatt agtgttcacg aatccgatta tctcggtagt
    28801 tcccagagta cttgttggct tagtagcagg cttggttttc cactggattt atcaaaaaaa
    28861 taaatcaata acgatcagct cagcctttgc aggggtactg ggttcattgg tcaatacagt
    28921 cttagtgtta ggattcatgg gattatttta tacaggtgca acggcagatg cctatggtgt
    28981 agactctgct cttttattta aaaccttagc aggggttgca gcaatcaatg ggataccaga
    29041 agcaataggt gcgggaatca taacaccatt actagcaaaa gcgttatttg cagcaacacc
    29101 gttgaaacca gaataatgac catcttttaa tagtaatagg acgcacgata gatagaaaaa
    29161 ggaagagagg agaggaaaac aatcgtcctc tccatctttt tacctgagca acaagcaaac
    29221 tagaaggggt aaaaaacttg gctaagaaac aagaagaaaa aaagaaacaa ataccgcaac
    29281 agcggacaaa aaaaggagta ctagcgggta tcttaggatt ggttacgaat ctgatcttgt
    29341 ttgtctctaa gctaatgatc gggctttttt caggaagtgt ctccattatg gctgatgcag
    29401 tgaacagtct atcagatact gcttcatcca tattgacttt gatcggattt aaaattgctg
    29461 caaagccagc agatcaggaa catccttatg gacacgaacg cttcgaatat atcagtgggt
    29521 tatttgtttc gatcatcatt acttatgtag gatttcaatt tttagagtct tctgctgaaa
    29581 aaatcatcca tccagaaaat gtctctttaa cacccatcgt cttttttgtc ctaatcattt
    29641 ccatcttttt gaaatttgca caaggcaaaa tgtatcaggt tattgcgcaa tccattgatt
    29701 ctgaaacatt gagagctaca gcaacagata gttataatga tgtctttact acactggctg
    29761 tattgctttc agcagcaatc gaatgggtaa ctggatggcg tattgatgga tatataggtt
    29821 ttgctttagc attatatatc ctttatagtg gattgacgat gcttcgaaat tttgtatacg
    29881 aactattagg aagtcgccca acgccagcag agattgaaga aatggaagag catttaaatg
    29941 cttgcgatga tatacttggc tatcatgatt tattggtgca taactatgga cccaataaac
    30001 gctttgcatc agtccacatc gaagtagatg aatcaatgaa tttgaatgtg gctcataaga
    30061 ttattgatct gatagaaaaa gactttaaaa agtctttgga tgtggaactt gtctgtcact
    30121 tggatccaat gcctgttgat aacgaaaaat actataatat cttgcggcag ttaaagcaaa
    30181 ttcttcttga aacaggtaca gggcttaata ttcatgattt tagagtaatt catcaaggaa
    30241 aaacgctgca atttgatgta gtggtccctg agaaattcca gtttgttgat acagaactag
    30301 aaagaatctt acgtagtcaa atagaagaaa aaatcggaga ttaccaattg gaaattacgt
    30361 ttgatcacaa ttatctttta tattaaatga gaaactataa aaagact
//
LOCUS       DACDIH010000028        28306 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDIH010000028 DACDIH010000000
VERSION     DACDIH010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.028"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            8..81
                     /locus_tag="IV281_001352"
     tRNA            8..81
                     /locus_tag="IV281_001352"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="IV281_001353"
     tRNA            101..173
                     /locus_tag="IV281_001353"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="IV281_001354"
     tRNA            201..274
                     /locus_tag="IV281_001354"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="IV281_001355"
     tRNA            288..361
                     /locus_tag="IV281_001355"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="IV281_001356"
     tRNA            385..474
                     /locus_tag="IV281_001356"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="IV281_001357"
     tRNA            487..560
                     /locus_tag="IV281_001357"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="IV281_001358"
     tRNA            579..654
                     /locus_tag="IV281_001358"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="IV281_001359"
     tRNA            656..726
                     /locus_tag="IV281_001359"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="IV281_001360"
     tRNA            740..813
                     /locus_tag="IV281_001360"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="IV281_001361"
     tRNA            838..925
                     /locus_tag="IV281_001361"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="IV281_001362"
     tRNA            947..1018
                     /locus_tag="IV281_001362"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="IV281_001363"
     tRNA            1041..1130
                     /locus_tag="IV281_001363"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="IV281_001364"
     tRNA            1143..1216
                     /locus_tag="IV281_001364"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="IV281_001365"
     tRNA            1235..1307
                     /locus_tag="IV281_001365"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="IV281_001366"
     tRNA            1311..1383
                     /locus_tag="IV281_001366"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="IV281_001367"
     tRNA            1388..1458
                     /locus_tag="IV281_001367"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="IV281_001368"
     tRNA            1471..1544
                     /locus_tag="IV281_001368"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="IV281_001369"
     tRNA            1556..1627
                     /locus_tag="IV281_001369"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     gene            1893..3086
                     /locus_tag="IV281_001370"
     CDS             1893..3086
                     /locus_tag="IV281_001370"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP8780370.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="IV281_001371"
     CDS             3216..4688
                     /locus_tag="IV281_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8780371.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="IV281_001372"
     CDS             4771..5559
                     /locus_tag="IV281_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP8780372.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="IV281_001373"
     CDS             complement(5626..6294)
                     /locus_tag="IV281_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8780373.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGIKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="IV281_001374"
     CDS             6409..8121
                     /locus_tag="IV281_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8780374.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9893
                     /locus_tag="IV281_001375"
     CDS             8124..9893
                     /locus_tag="IV281_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8780375.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10098..10664)
                     /locus_tag="IV281_001376"
     CDS             complement(10098..10664)
                     /locus_tag="IV281_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8780376.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(10664..11620)
                     /locus_tag="IV281_001377"
     CDS             complement(10664..11620)
                     /locus_tag="IV281_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP8780377.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(11620..12276)
                     /locus_tag="IV281_001378"
     CDS             complement(11620..12276)
                     /locus_tag="IV281_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP8780378.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            12646..14664
                     /locus_tag="IV281_001379"
     CDS             12646..14664
                     /locus_tag="IV281_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8780379.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFSARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            15091..17505
                     /locus_tag="IV281_001380"
     CDS             15091..17505
                     /locus_tag="IV281_001380"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP8780380.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            17853..19406
                     /locus_tag="IV281_001381"
     CDS             17853..19406
                     /locus_tag="IV281_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8780381.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(19787..21571)
                     /locus_tag="IV281_001382"
     CDS             complement(19787..21571)
                     /locus_tag="IV281_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8780382.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(21586..23307)
                     /locus_tag="IV281_001383"
     CDS             complement(21586..23307)
                     /locus_tag="IV281_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8780383.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            23407..24168
                     /locus_tag="IV281_001384"
     CDS             23407..24168
                     /locus_tag="IV281_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8780384.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            25135..25403
                     /locus_tag="IV281_001385"
                     /pseudo
     CDS             25135..25403
                     /locus_tag="IV281_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            26075..>28306
                     /locus_tag="IV281_001386"
     CDS             26075..>28306
                     /locus_tag="IV281_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780385.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         8925 a         5058 c         5989 g         8334 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaattccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa taaagaggtt
     9901 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag cttctcatgt
     9961 ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt gaataaagag
    10021 gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac atatcattta
    10081 tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg cggctggttc
    10141 ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag tttggaacaa
    10201 aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta gacgacaagg
    10261 atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt gcgtgtagtc
    10321 tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt gaaaatccct
    10381 gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc ttgtagaaac
    10441 aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc atcgattgct
    10501 tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc tgctagaaaa
    10561 accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg ttgcaagatt
    10621 tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg ctcacctttt
    10681 tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca tcaattgcat
    10741 tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc cacggcgcat
    10801 cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg tcgcaaatga
    10861 cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag tcccgcaaca
    10921 ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata tctgaatcta
    10981 aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt aatccgttga
    11041 tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca tctaaataag
    11101 tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt gtttgcaatc
    11161 caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca actacttcat
    11221 ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt aaattgacta
    11281 cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta aaattttgga
    11341 aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg atttctttct
    11401 ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa ccagatacac
    11461 tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag tcaaatcctc
    11521 catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg taattccaag
    11581 aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct tctttctgta
    11641 agagttatct atttgttttc ttttttctct atacactgga taagtcaggc acagccaaga
    11701 aagactgagt aagtagccgc cgatgatatc agtaggaaaa tgaacgccca gataaatgcg
    11761 gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg tgcgtaaggt
    11821 tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa tcatactgcc
    11881 tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg ccaatgaagg
    11941 acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa ttacaataaa
    12001 tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc ataaagccgc
    12061 acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa agagttggta
    12121 gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat cgaaaccaga
    12181 aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga caagaaagaa
    12241 gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt gaagcaatga
    12301 cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac caatgtatga
    12361 ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga aagacatttt
    12421 ttggtaatgc aaatgaaatg ttagtgaaat aaaatcagtg tttttcgaaa tccttttaaa
    12481 caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt acggtaaatt
    12541 acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat attaagagaa
    12601 gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga aaacattgca
    12661 agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa acgaggggca
    12721 gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt acagccggtt
    12781 cctgttcaag cggatgaaac acctgttcag ttcagcgcaa gaatcaatac agcggcattt
    12841 atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct atatgcatca
    12901 gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt atctcaagca
    12961 ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt ctacatggat
    13021 acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg acaatatcct
    13081 tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc atttggcaac
    13141 ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga tgcgacagct
    13201 tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa taatatcatc
    13261 gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg cggtggagcg
    13321 actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag caattctggt
    13381 aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg tgattctgtt
    13441 tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg gaacaacatc
    13501 caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca ggaaaatggc
    13561 tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg tacatcaaac
    13621 ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt ttggaagatc
    13681 tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat caagaacaat
    13741 ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa tgctggagca
    13801 aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc aagttcttct
    13861 aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg gagcgtctca
    13921 aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca aaataacttt
    13981 atctacccag gacaacaact gactgtttca aacagtggat caacaaacag cacgtcaggt
    14041 aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg cagcagttcg
    14101 tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc aaataaaaac
    14161 ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt catttatcca
    14221 ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg ttcaactact
    14281 tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc atccactggt
    14341 aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa caaacatcat
    14401 atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat ctatccagga
    14461 cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc agttactggt
    14521 tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga cagtcatgga
    14581 attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat ttatccaggt
    14641 caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt gaattccaca
    14701 aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg aagtagtaaa
    14761 aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc ttttgaactc
    14821 gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg gccgttacaa
    14881 cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta cgtcctataa
    14941 aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag aaaacaaagc
    15001 aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat aatagaaaca
    15061 agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga aattgagaaa
    15121 aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga tcctgaaaag
    15181 ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct acatgtaggg
    15241 catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag tcaaggatac
    15301 aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca atatgcgcta
    15361 gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt ccgacgccaa
    15421 atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac tgatccggag
    15481 tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct agcttatgaa
    15541 gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa tgaagaagtg
    15601 atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat gcgccaatgg
    15661 atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt agttgattgg
    15721 ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg agcaaatatt
    15781 gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg accagatact
    15841 ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag agaaattacg
    15901 acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa aaaatcagat
    15961 ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc ttatgcaatc
    16021 aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt atcttcatac
    16081 ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga attcgctcaa
    16141 actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa agctgcttac
    16201 acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa acaagaagca
    16261 atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga agtcagctat
    16321 cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat cccaatcatc
    16381 cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt acggttgcct
    16441 aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa tatcgaggag
    16501 tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac gaatacaatg
    16561 ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca taacaaaaaa
    16621 gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta cattggtgga
    16681 gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt cttatacgat
    16741 atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg aatgatctta
    16801 ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc tgatgatgtg
    16861 gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg gccattagat
    16921 gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt ggatcgtgta
    16981 tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac attgaatgat
    17041 ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga ctatgaaaat
    17101 cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc ctataaagtg
    17161 gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc gattgctcct
    17221 cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc ttacgcacca
    17281 tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt tttccaagta
    17341 aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga tgagctagaa
    17401 aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac agtcagaaaa
    17461 gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc tctttgtcaa
    17521 taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca ttctgtctca
    17581 gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga ttttcatgtt
    17641 ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct ctgtaatcat
    17701 caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga aattgagagt
    17761 acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga ttttgacgca
    17821 taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt attatcgaag
    17881 tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa agaataatgt
    17941 tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct gtatcgttcc
    18001 tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa ttaaataatt
    18061 tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt gtttttcttc
    18121 tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg cttagtcaaa
    18181 gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat ctttattttg
    18241 agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag ttattgctga
    18301 gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc ggaatgaaca
    18361 cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag tcttcgcctg
    18421 gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc attcttttag
    18481 ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat ataaatgctg
    18541 cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca aatgaagggt
    18601 tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat gatctcaaac
    18661 ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat tgggtatttg
    18721 tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg gggctgattc
    18781 ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca tggtttatct
    18841 attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa acatggcaaa
    18901 cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta ttttatggca
    18961 acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact ttgtacattt
    19021 acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg atcaatcagc
    19081 cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca cgtgaaagtt
    19141 tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg attccggaaa
    19201 actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact caaataagcg
    19261 cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat gcagggaaaa
    19321 tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc gttcagcaac
    19381 tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg ttcagcaccg
    19441 agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt gtaactcgtg
    19501 attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc gtattttaca
    19561 agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt aaaaagggat
    19621 tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc caaaaagtat
    19681 cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa aagctatcga
    19741 tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac gctatctgaa
    19801 attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca gaatgtgttc
    19861 ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt tcaatcgttg
    19921 acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc attgcttgct
    19981 gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa ataacgacag
    20041 gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct tgagataacg
    20101 tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga ataaagtgat
    20161 cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc attttcccgt
    20221 aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa accatcccaa
    20281 aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg aattcgatct
    20341 ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt tttcctgcac
    20401 cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg acttgattca
    20461 tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt tttcctttcg
    20521 gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca tctaatatct
    20581 caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca taagaagcgg
    20641 tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag aagccgatcg
    20701 tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg aaggcgagtt
    20761 gattaatgaa acgaatggcc ggatagatag caaagttcat aaattgagca cgtttaaaag
    20821 cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct tgctgattga
    20881 atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt aaaacaccca
    20941 gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta gttgtaacaa
    21001 ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg atataaaaca
    21061 gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg atacctgtca
    21121 ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg tcacctactt
    21181 gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt tcttttcgca
    21241 ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata aatgaagtga
    21301 tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat gcttgtttga
    21361 tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag agatcatcga
    21421 tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat aaactgcata
    21481 gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa cgtatcaaag
    21541 cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata ttccttgtga
    21601 ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag taccttgtcc
    21661 aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg tactgaggcg
    21721 ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg ctcttaatgc
    21781 tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga tctctgctgg
    21841 tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat aattagaacc
    21901 gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt cttctaattg
    21961 agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc ccatctgcaa
    22021 gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac tgatgtggga
    22081 gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta cttttcctat
    22141 cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag atccggtccc
    22201 gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt ctagaactgg
    22261 atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat ttgtctgatc
    22321 aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct ctgcttgcag
    22381 tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca ataccatagc
    22441 tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag tcacgacacc
    22501 gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag aagcataaac
    22561 gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt tcactttgat
    22621 catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat atgattgatt
    22681 atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga tggtgatccg
    22741 atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca gtcgataaac
    22801 aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata gagaataacg
    22861 aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca tttggcaaaa
    22921 taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca aaagagatcc
    22981 agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga aatatttctc
    23041 ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag aggcaactgc
    23101 agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt ttataactga
    23161 ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg gtacacctaa
    23221 tgtaccggcg atttgtaaac agatcatcac aaaggtaagg agagcgagtc ctttgttttt
    23281 ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat acttttcgat
    23341 tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa aaagaggtga
    23401 gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga tttgccaaaa
    23461 tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca agaagagaat
    23521 gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt tttttataga
    23581 aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt gacggagtta
    23641 tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt gatgaaacaa
    23701 aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga tacaaacgag
    23761 gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga gtttgaagat
    23821 attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat gagtatgcca
    23881 gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt acatttattt
    23941 actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt cttattgaaa
    24001 tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag aatgtatgaa
    24061 aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac ggatgaaaac
    24121 aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga aatatgtgtt
    24181 taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct ggttcgatta
    24241 cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt tttagaggaa
    24301 atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg taattgtcct
    24361 cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat ggtgaactga
    24421 gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat gagaatgata
    24481 aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac attattacaa
    24541 tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt aatcaaatag
    24601 tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt tttgcttata
    24661 aaataattat aagtatttag agatttttct tctaacattc ttcaaacaag aaaaatcaca
    24721 aaggttattg ttatttttat atattctttt taagaatgat tgttttttta tagaaatttt
    24781 aaaaaataaa acacgttatt gatggatagt tagtttgtta tttggtaata ttatagaagt
    24841 tgactggtag tgatttattt ttcgtcgttt tataaaaatg ttattttttg gaaaaattca
    24901 caaatatgta acgaatgttt ttctggaatt tgctgtgtta aaaagcagta tgactaatat
    24961 aaatactatt gtcatttatg caatttttta tggatagtaa ttttagaggg gaatcaatga
    25021 aggaagaata gaagggaaaa atagttgcaa atacgccagt tagcaaaaat agcaatacat
    25081 cttcaagctt taaaattcga gagaatcatc ttggtagtgg aatcaactac aacaaaagga
    25141 ttaaaccaat taatagatag aattaccaat ggaaaaatag aaaaggtagt tattctatac
    25201 aaaagttgtt taattcgctt cggttatgaa cttagtgaaa ccctttgcaa tatatggtac
    25261 agaatataga aattattgac attacagaaa atgatgaaga tttaatacag attgtcacta
    25321 tttgtagata ccttttacaa ggaaaccgag ccaataaagc taagaagatg attaaggagt
    25381 tgatggaaga cgatacaagt taagaaagtg aaactacgcc caaataaaga gcaaaaagag
    25441 taattggacg tggaaaatgc aagaaatgaa ccatcgattc ggtggacagt ttattagtaa
    25501 tgacggtttg agaacatcca tgacgagaat gaaggaacat cctaaatgcg tttagttgaa
    25561 ccaagtgcca aataatatgg ccaaataagc gatcagttat cgatgaatcc caagtattct
    25621 aaactatata ttcattttga tgctaagcat tgatatattt ttttgggatt gatgaaggtt
    25681 aaccaataca gggactcact gatgaaattc tagagtctga ccttggtatc aacgatttag
    25741 tgattgtttc taatggaaaa gtataaaagt atataagtac attaacaaaa taactattag
    25801 ggttacgctc cagcttgtgc aaggcactgg caggaggaat cgtgaaatca taattatggt
    25861 taagccacac ggacaactaa aagtcataca aagctatcga atcaatcaac agaagacaat
    25921 cggcaatcat tagctttgtt tacagcttac attgcaacgc ttgagttgaa ccgaaaatac
    25981 gagggaggta acgcagccat tttgagttgg ggaatctttt tagaaaatta tcgggtacct
    26041 tgttgtttag tcaaaattat aagggggagt aatagtgagg aacaaatttc ataaactgtt
    26101 aagtatgttt ctagttatgc ttattgtgat caatcaattt gggaacataa cggttctagc
    26161 tgagtcttta gcagaacagc cagagcgttc gctgacgatc gaagttgatc aagaggaggc
    26221 tgctttgggt gagcagttcc agttgcgagt cagaggaaca ccagacctgc taaatgcatt
    26281 ggtcattgat aagagtgagg atttgattga aggggaagtg aagcaactca atgattacga
    26341 acgtctgatt acttattctt cccagcattc aggtaaatac gaattgaccg caagaagcgg
    26401 tgatattcaa tcaaatacgg tctcaataga aatcagtgta ccagaagaaa gcacaagtga
    26461 gtcttttaat gtaccagaag aaagcacaag tgaatctttt aatataccag aagaaagctt
    26521 aagtgaatct tttaatgaag ccagatcatc tgagccatct ataacgcagg aatctcagat
    26581 tgaaagacaa gaggagctct tgaattatga catcggcatc tctgctgagc gccccacttt
    26641 gccgagcagg catacacaaa cgttaaaggg atacattcaa ggaggcgctg gcgatattct
    26701 tgaagaatat ggggagatgg tcgtacgagt tcctagagca tcgattggct taattgagtt
    26761 cattagagtg gaggtcgctg atgattcacc tttaaagcca ctggcatttt accaagaagg
    26821 cacagacgtt gtttttccat ttacctttga ttatgaagga ttaaatgaag ccgctcagtc
    26881 gtatacattc agtatcacgt ttgaagttgc tttctttcaa tcaggcggta ctgagccaaa
    26941 taatgacttt caagtgagtc ttacttatgc tgagcgagca gcaaacacgg aattttcaat
    27001 tacgcctatt ccttcatcaa gacccatttt ttcgaaatta tactatggct ctagagaaat
    27061 cgacagccaa ggaaatcttg ggaatctgct gattgacggg caagacgtga ccaacagtaa
    27121 taaatttatt ttgcaggtaa attatgaagg atcacgaaaa gacggtgtag tggttaccga
    27181 tgtactaccg caaggaacaa ctttggcacc gaatccgact acatggagtt ccactggaaa
    27241 tagagaacct atttcaggtg gggttcggat cgcagaagta acgaatccag gtgaagcaaa
    27301 cgttagaact cattatgttt ccgaggaatt tgtggacaaa gtatctttta ataaggaaac
    27361 gaatacactt gttgtggact ttggtaatct agcagagggt caatcgtatt ttattgaata
    27421 tgctcttgaa gtgaccgatg tcgacttgtt caatgaagga agacaagagg atgggttttc
    27481 tcggaatcga gcgagtatga cctacaataa tcaggtaatg agccgagact accgcttgag
    27541 aatccctgaa agtaatcttc cggcaatctc tttaagaaaa agtgtggaca aaaatgttat
    27601 caatctcaat gaaaatgaac taacgtatac tctgcatgtc tcgatgtttt ctggaacaag
    27661 aaatacgctt gttattgaag atgttttgga agaaggcatg gaatttgtgt catttctatc
    27721 agatattccc tctttcattg atgccgaagt caataatgga atagttcgtt tcagtgcgaa
    27781 taatacgatt ggtggtccga caagttttac cctctctttt aaggtcaatg tagaggcgta
    27841 tgcggtcggc gatcaaattt taaactttgc aacgcttgtg aatagtgaca atcgattgaa
    27901 tacgagtact gtaagaacaa ggaaaatcga tgggcgtatt aaagtaatta aaactgatga
    27961 gaatggccaa aggctggcag gagcaaaatt tgaaatttta tcaagcgatt cacaagtgat
    28021 acaagaaggt ataacggcag aaaacggaga atttttaagt gaaccgcttc ctgtaggtag
    28081 ctatcaagtc cgagaaacag aagcaccgga agggtatctt ttagatgcga cggtacatga
    28141 tgtttttata aatgaatact ccatagaaga agtgacgtta accattgaaa acatccttga
    28201 tacgggtggt gttgaactaa ttaaacatgc tgaaggcagt ggtgaagtcc ttcaaggagc
    28261 tgtttttgag ttacagaacc gagaaggaga aacgctacaa accggt
//
LOCUS       DACDIH010000029        27747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDIH010000029 DACDIH010000000
VERSION     DACDIH010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.029"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..107)
                     /locus_tag="IV281_001387"
     rRNA            complement(<1..107)
                     /locus_tag="IV281_001387"
                     /product="16S ribosomal RNA"
     gene            26..169
                     /locus_tag="IV281_001388"
     CDS             26..169
                     /locus_tag="IV281_001388"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780386.1"
                     /translation="MEKEAFDLHVLGTPPAFVLSQDQTLIKSSNNLAIVKLNLFASIL
                     LAC"
     gene            complement(787..2283)
                     /gene="lysS"
                     /locus_tag="IV281_001389"
     CDS             complement(787..2283)
                     /gene="lysS"
                     /locus_tag="IV281_001389"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP8780387.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2394..3398)
                     /gene="dusB"
                     /locus_tag="IV281_001390"
     CDS             complement(2394..3398)
                     /gene="dusB"
                     /locus_tag="IV281_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP8780388.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3399..4286)
                     /gene="hslO"
                     /locus_tag="IV281_001391"
     CDS             complement(3399..4286)
                     /gene="hslO"
                     /locus_tag="IV281_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP8780389.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4503..6614)
                     /gene="hflB"
                     /locus_tag="IV281_001392"
     CDS             complement(4503..6614)
                     /gene="hflB"
                     /locus_tag="IV281_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP8780390.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6704..7249)
                     /gene="hpt"
                     /locus_tag="IV281_001393"
     CDS             complement(6704..7249)
                     /gene="hpt"
                     /locus_tag="IV281_001393"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP8780391.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(7249..8694)
                     /gene="tilS"
                     /locus_tag="IV281_001394"
     CDS             complement(7249..8694)
                     /gene="tilS"
                     /locus_tag="IV281_001394"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP8780392.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8814..9284)
                     /locus_tag="IV281_001395"
     CDS             complement(8814..9284)
                     /locus_tag="IV281_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP8780393.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9330..9755)
                     /locus_tag="IV281_001396"
     CDS             complement(9330..9755)
                     /locus_tag="IV281_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP8780394.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9822..10091)
                     /locus_tag="IV281_001397"
     CDS             complement(9822..10091)
                     /locus_tag="IV281_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP8780395.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(10102..11697)
                     /locus_tag="IV281_001398"
     CDS             complement(10102..11697)
                     /locus_tag="IV281_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8780396.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11712..15233)
                     /gene="mfd"
                     /locus_tag="IV281_001399"
     CDS             complement(11712..15233)
                     /gene="mfd"
                     /locus_tag="IV281_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP8780397.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(15250..15810)
                     /locus_tag="IV281_001400"
     CDS             complement(15250..15810)
                     /locus_tag="IV281_001400"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP8780398.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            16163..17137
                     /locus_tag="IV281_001401"
     CDS             16163..17137
                     /locus_tag="IV281_001401"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8780399.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17307..18707
                     /locus_tag="IV281_001402"
     CDS             17307..18707
                     /locus_tag="IV281_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP8780400.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18785..19507)
                     /locus_tag="IV281_001403"
     CDS             complement(18785..19507)
                     /locus_tag="IV281_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8780401.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19586..20092)
                     /locus_tag="IV281_001404"
     CDS             complement(19586..20092)
                     /locus_tag="IV281_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8780402.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            20215..22014
                     /locus_tag="IV281_001405"
     CDS             20215..22014
                     /locus_tag="IV281_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP8780403.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(22082..23656)
                     /locus_tag="IV281_001406"
     CDS             complement(22082..23656)
                     /locus_tag="IV281_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780404.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23911..24420)
                     /gene="mreD"
                     /locus_tag="IV281_001407"
     CDS             complement(23911..24420)
                     /gene="mreD"
                     /locus_tag="IV281_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP8780405.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(24424..25278)
                     /gene="mreC"
                     /locus_tag="IV281_001408"
     CDS             complement(24424..25278)
                     /gene="mreC"
                     /locus_tag="IV281_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP8780406.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVASVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(25433..26071)
                     /locus_tag="IV281_001409"
     CDS             complement(25433..26071)
                     /locus_tag="IV281_001409"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP8780407.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(26072..26722)
                     /locus_tag="IV281_001410"
     CDS             complement(26072..26722)
                     /locus_tag="IV281_001410"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP8780408.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(26735..27634)
                     /gene="rsgA"
                     /locus_tag="IV281_001411"
     CDS             complement(26735..27634)
                     /gene="rsgA"
                     /locus_tag="IV281_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP8780409.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
BASE COUNT         7618 a         5985 c         5049 g         9095 t
ORIGIN      
        1 tctttttccg gtggagcaag ctccggtgga aaaagaagcg ttcgacttgc atgtattagg
       61 cacgccgcca gcgttcgtcc tgagccagga tcaaactctc ataaaaagtt cgaacaatct
      121 tgcgattgtt aagctcaatt tgtttgctag catattgctt gcttgttaaa aatgtttgtt
      181 gtcttgaatt gaatcaagac gccctacaca tttggtttgt cttactttgt tcagttttca
      241 aaggtctact tcatcgagag tgtttcccga ttcatttttg gctgccgtag caacttttat
      301 atcatatcag agagacaagc gattgtcaac tcttttttaa aagttttttt cgatttcgtt
      361 tttcgaaatc tttcggcaac tcatttatca taacttatcg tttgttattt gtcaagaact
      421 tttttgaagt ttttttcgat agaatcaagc ttcgcaagtg gttctttttt aacgactcag
      481 ttatcttatc atgtcgttag taacatgtca ataacttttt tgttatgttt ttgaataaat
      541 attttcttct gtcttttgga caagtaataa tataccaact aaatttagct tggtcaacga
      601 attttttgta ttttttcaag tttttttcaa taaacgatta ttcataactt ttctttcttt
      661 atcgttcgtt ttcgtcttct ttaatgtcta gaatagtcgg cattagcttt tgaactttcg
      721 ttattattta ttcctttcta cacagcaaaa gctgcctaag gaattcctta gacagctttt
      781 attcgattat ctcattgttg ggaatagcaa aacatcccga attgattgtg catcagttag
      841 caacattact aatctgtcga tcccgatgcc tagaccacca gttggcggca taccgtattc
      901 taaggcttcc aagaagtctt cgtctacacc gtgagcttcg tcatttccta gttcacgttc
      961 tttttcttgc gcttcgaatc tttctctttg atcaattgga tcgttcagtt ctgtaaaggc
     1021 attagcaaat tcgcgcccca ctatgaatag ttcgaatcga tctgtgaatc gaggatcttc
     1081 tggatttttc ttcgctaaag gcgaaacttc gactggatgt ccgtatacaa acgtcggttg
     1141 tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg ataacatgtc cgacacccat
     1201 agcttcggtt acttctacat tgtgtttttc ggctaaagct aatgcttctt ccaatgtcat
     1261 ttcttgccag aagtctacac cagtttgttc tttgattgca tctaccatat ggatgcgttt
     1321 aaagtcactt tctaagtcga cagcttgtcc atcatatgtg atttttgctg ttcctagtac
     1381 tttttcagct gcgttacgga taataccttc tgtcagattc attacatcat tgaaatcagt
     1441 atatgctgtg tatgcttcta acatcgtaaa ttctggatta tgtgtagtat caattccttc
     1501 attacggaaa acgcggccga tttcataaac tttttccatt ccgccaacaa ttagtcgttt
     1561 taaatgaagt tctagcgcaa tacgcaaata taaatcaatg tccagtgcat tatgatgcgt
     1621 gataaatggg cgtgcggcag ctccacctgc ctcattgtgc aatactggtg tttctacttc
     1681 aatatagcca ttgccatcta aatagcgacg gatttcgctg ataatttggc ttcgtttcat
     1741 aaatcggtca aagctttcgc gattactgat taaatccaag tagcgttgtc tgtagcgttg
     1801 ttcaatattc gttaagccat ggtatttatc tggcagtgga cgaagtgctt ttgacaaaat
     1861 cgtgatttct gaagctttga tagaaacttc gcctgtgtct gttttcatga tttgtccagt
     1921 tacaccaaag aaatctccaa ggtctgcatg tttgaatact tcataggcct catcacctac
     1981 ttggtctttt cttacataaa tttgaatttg gccttcacga tcttgtagat gcgcaaatcc
     2041 agctttccct tttcctcggc gagtcatcat acgaccagct acactagcgg tcaatcccat
     2101 ttctgctagt tcttcttttg ttcttggatc aaatagttca tgcaattctt ctgagttgtg
     2161 cgtgcgttca aatcgttttc caaatggatc gattccatct tcacgcaatt gttccatttt
     2221 ttgacgacgt acaagcattt gatcatttaa atcttctact tgatgttgtt gttcctgtgt
     2281 cacaaaagtt cctccattct catgttaatc tctttcctat aatgccaatg aaacacaaaa
     2341 aaagcaagcc gaacttggaa tttcggcttg aaaattcacg aaaaacttag aaatcacaac
     2401 gtttgtttca atagtacttt ttccgctttt tctacaaatt gatccagcag gtcattgatt
     2461 tcttgctgtt tttctgtctg attgattgct actttcactt tcgctgctcg aggaatccct
     2521 tttaaataat aagccgcatg tgttctgaat tctctacaag ctactttttc tcctttgaga
     2581 tctgctaagc gttgtaaatg aagtttagct gtagcaattt tttcggctgg tgttggttca
     2641 ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt gaatcatcca tggattgccc
     2701 agcgctgcac gaccaatcat cacaccgtct gcaccgacat agtcaagcat ccattttgca
     2761 tcttctggcg ttttgacatc cccgttcccc ataaatggga tcgtcaaatg ttttttcact
     2821 tctttcaata cttcccagtc cgcttttcct tcatacatct gaacccgtgt acgtccatgc
     2881 atagcgatgg cacttgctcc agctgattgt gctgctaatg cattttctac agcaaagacg
     2941 tgctcttcat cccaaccaat acgcatcttg attgttactg ggatatttac tgctgaagaa
     3001 acagcttgga ccatttcgta tactttctca ggatctaaaa gccattttgc tcctgcttct
     3061 gctttgatta ctttatttac tggacagccc atattgatat ctattatatc ggcagctgtg
     3121 ttttcttcga caaattgagc agcttcaact agactatctt tatttccgcc aaatatctgt
     3181 aaacttaatg gatgttctgt ttcttcaatg tggagcatct ctaaagtttt tttgtttctg
     3241 aaatgaatac cttggtcact gatcatttca catacgacta agcccgcacc aaattctttg
     3301 acagtgactc gaaatgctga atttgttatt ccagccatcg gtgcaacaac gacacgatta
     3361 gggatttcca cattgccaat tttccatgtg tgttccactt aaatccctcc taaggcttct
     3421 ttcttcaatc catctaattc ttcttcgcta tacttgtatt tgttattaca gaaagaacaa
     3481 accgcttctg caccgtgatc ttcgtctatc attgcttgga tttcttctgc acctaacgcg
     3541 ataattgctt ctgcaaattt ttcttttgaa cagtcacatt tgaattgaac aggcattttt
     3601 tccaaaatgt tcaattcttc tgtaccaagc aatcctttca atatttcttc tggtgtctgt
     3661 ccttcatcta agagggtaga gattcttggt gtcgttttta attgttcttc gatcttgctg
     3721 attgtttctt catcggcacc tggcatgatt tggatcatga atcctcccgc cgtcttgatg
     3781 ctgtcgtcag tattgaccaa tacactcagt ccaacagctg aagggatttg ttctgagata
     3841 gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa caatcggcgt ttgcccagaa
     3901 aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca ttccttgtgt tccaactgct
     3961 ccccgcacat cgattttacc ttcactattt agaggaagac tgagatgcgg atttttgatg
     4021 taacctttca catctccctt tccatttgca tctactacaa ttcctgttgc tggaccatcg
     4081 ccttggattc gaacagttag tttgtcttct cctttgagcg tcgcaccaag tagcagacta
     4141 ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc aagtatcatg acgtctttgt
     4201 gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc ggacgaatcc gtcgtgagca
     4261 agtgccttaa ccaaataatc tttcatttgt tatgtactcc tttttccgtt tttaaactca
     4321 tagtgaatca tactagcaac tactgctttt ttcaactttg gaatgcttct tttaaaaata
     4381 attcagaatc atctagaaaa caggacatca ccctatttac aacttaaata aagaaatctc
     4441 ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc agatcaattg acctatattt
     4501 ttttatttgt attgatcgtc tgaatgatta tcttctgatt gttgagaatc tgaatcatgt
     4561 tctgctttat tttcctcatt cagttcatgt tttgcttctt caaaatcacg tttttcttcc
     4621 gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg cttcttcaaa agttttcgct
     4681 tctttttcgc ttggataatc atttgactca gtaccttcag gcatcacacc gtgttcaaac
     4741 aatgacttga ttgctttggc atccaatgtt tcgaattcca aaagtttttc agcaatcagt
     4801 ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt tatcatgagc ttccatcaag
     4861 atttttcgta cttcttgatc aatctcgaaa gcgacttgtt ccgagtaagc ttttgtttga
     4921 ccatagtcgc ggccaacaaa cacttgatgg tttccttcat attgaacagg acctaaacga
     4981 tcactcattc catattctgt taccatgctt cgtgctagag ctgtcgcttg ctcaaagtca
     5041 ttggaagcac cagttgattg gacattgaag atgatttctt cagctgtacg tccaccaagt
     5101 aagcctacga tttgttcaaa catatcttct ttcgtcatca agaactgatc ttcttttggt
     5161 aaagcgatca tatatccgcc ggcgcgtccg cgagggatga tcgttacttt gtggacgacc
     5221 cgagcgcgac tcaatactaa tccgacaatc gtatgaccag cttcgtgata agcaaccatt
     5281 tcgcgttctt ttttgctgat tacgcgatct tttttagctg gacctgcaat cacgcgatct
     5341 tccgcttcat cgatatcaga agcatcgatt ttctttttat ttcttctagc agctactaac
     5401 gctgcttcat tcaagacatt ttctaaatct gctccggcaa aacctggtgt ctgttgagcc
     5461 acaactttca agtcaacgtc atctgccatc ggtttattcc gtgcatggac tcgcaagatt
     5521 gcttcacgac cttttacatc tggacgtcca actaaaatct ggcggtcaaa acggcctgga
     5581 cgtagtaacg ctgggtctag tacatcagaa cggttggttg cagcaatgac gattactcct
     5641 tcatttccgt cgaagccatc catttctaca agcaattggt tcaatgtttg ttcgcgttca
     5701 tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta ccgcatcaat ttcatcaatg
     5761 aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca aatcacgaac acgacttgct
     5821 ccaacaccga caaacatttc tacgaaatca gaacccgaga tcgagtaaaa cggtactcca
     5881 gcttctccgg caacagcttt tgctaataag gttttacctg ttcctggagg tccttctaaa
     5941 agtacacctg ccgggatacg ggcacctaat tcaacaaatc ggcgaggatc tttcagaaat
     6001 tcaacaactt caactagttc ttgtttttct tcttccgcac ccgctacatc agaaaaacgt
     6061 acgcgattag cttttttgtc tgcttctttg gcttttgatt taccaaagtt catgacacgg
     6121 ccgccaccgc caccgccgcc gccttgttgt cccatcatca tgtaaaagaa aaagatgatg
     6181 attacgattg gcaagaagct aagcaatagg gaaacccata taccgctgct tgattcttct
     6241 tttactgttg tagcaacttt gttttctttt gccagttctg ttacttggct caatgttgta
     6301 tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg tatctgttga acctaagata
     6361 gctaacccac cgctgttagc gacggtctgc tgttctttgt attcaccagt gatcttatat
     6421 acaccatttg caggctgcac ttcaaattct ttgatcttgc ctgcttgtag ttgttctgtg
     6481 aattttgagt actcgatatc tgacgatcgc tggccgttat taccaaaaat gaaataaacg
     6541 accattacca tcgctaagat tacgaggaca taatacaagg catttttcat catgccgttg
     6601 ttctttttgt tcatagttgc cctccctaag cgtcaattta atttaacagt tttttatcaa
     6661 tcctgaccat tctattgtac cataatttaa ttgagataca gcattaattt gattgataaa
     6721 cactaggttt caatacaccg atgtacggaa ggttacgata tgcttctgca taatccaagc
     6781 cataaccgac cacaaattca tttggtacgt tgaagccaac ataatctgct tcgatattta
     6841 ctactcttcc ttcaggtttg tcaagtagtg taacaatttt gactgatttt gctttgcggt
     6901 atttgaaaag atccactaaa taagcaagtg ttcttccgct gtcaatgata tcttcaacga
     6961 tcaacagatc gcgaccttct acattcgtat ctaagtcttt gacgattttt acttcccctg
     7021 acgagactgt tgcattcccg tagcttgaaa catccatgaa atccaattcc aaatacgtat
     7081 cgatagaacg aacgatatct gccatgaatg gtaccgctcc ttttaagaca cctactacta
     7141 ggggattggt gttttggtaa atttctgtta actctttgcc tagttctttg caacgaactt
     7201 gtatctcttc ttgtgagatc aaaattctct caatatcctt ttccaacatc aggttctcct
     7261 tccaattgct tctttttgat atttgtaaag aagtatgtag tgtattttat cagtttcttc
     7321 acgaatactc aaatatgaaa aggcaaaagg tatcaagcta tatatatttc ttttggcatc
     7381 tgtaatgatc caagcatgct gcctttgtga attaggtact ttctgatcga taaaatatct
     7441 agatactttt tttgtgagat tctctgttaa tttaatgcgg tctcctgctt ttcttttttc
     7501 gacatacagt tgcacatcag caggaatcca aatgtcatga gaaaatagag accatgaatc
     7561 tgttgctttg atgtcccctg cattttcagg tttgaccaga ccaatccatt ctgtttcatt
     7621 caaatatatt ccttgttctg gaatcagcaa caagcgctgg tcttctgtct ggctagttgc
     7681 ttgtttttct tttttagaga gtacaacaaa atgatactct cttttcagta tccaattatt
     7741 gccaatactg agttgccatt ggcttttttt ctgttgccac tggtctaaaa tcgactccat
     7801 ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct ttgtcgaaaa aggagtaaaa
     7861 aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt tctgcagaaa atcgaaattg
     7921 ttcctcccac tgttcgactt ccttttcgat caactgtccc atatattttt gaatgacttg
     7981 gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt ctcaacgcct gtgtattttc
     8041 ttgtttcaat tgaggaacta ccaaatgacg aagccgattt ctctggatat ctaacaattg
     8101 gtttgtctga tcctcaaagt aaacaagctg cgattccgct gcataagtat ataggtcttc
     8161 tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta gcaaatggtt gtacttcttt
     8221 gattccagaa aaagtattta actgtccccc acgaacgatt ttcattaaca ccgtctcgat
     8281 ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat tgtccttctt ccatcacgcg
     8341 agtgaattct tgataacgaa agttacgagc ttctgtttct atggcggatt tggctggatc
     8401 ttcccaaata cttacatata aagggagatt cttctcttca caatatgagc gaagataggc
     8461 agcttcttct gctgattctt ctcttaattg gtgattgatg tggacgatac ctaaagaaaa
     8521 accgatttgg cgttgcactt gtaaaagtaa atggagcaaa accatcgaat caacaccacc
     8581 tgataccgct gctaaaattc tcgatccttg ttgccagaat ccttgttcat tcccgtgccg
     8641 aataaatgac tgctgtaata gcggtgcttt tttcccgcct ttttcaacgt tcatcctttc
     8701 cacacccttt cttcctaaaa aaaagagagc gggaaaacgt ctaaaacgac aattaacctg
     8761 ccctctgttg atactatgac ccaaatagtc gcactatcat tatttgcggc tttttagctg
     8821 cgacgtccgc cacggccacc gcgctttcct tctgtgtttc gcttcaatga agataagcgg
     8881 tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga agtcttcttt ttgagctgaa
     8941 ggttgactgc gagaaaaaga ttttttctgt gctggacgat ttcggttgtc acggggatct
     9001 ttttgttgat attttttagg acggctttct tctccagatt cagcaggttg ttcctgcgct
     9061 ttccggatag atagtccgat ttttccatca tcatttactg aaataacttt aactgttact
     9121 tcatctccta cacttaaaac atcatggatg tcttttacat agccatttga cacttcgcta
     9181 atatgaacta gacccgtttt tccagcacct agatcaataa acgcaccaaa atttgtgatt
     9241 cctgatactt ttccttgcag ttttgctcct acctcgattg acataaaaaa aatgttcctc
     9301 ctaattattt ccctttttta tattcctgct tattttgcag ttgtagaact agttgattga
     9361 tctgtttcat ttgtcgagct gtctgtattt gattctaata taggataaac tttttcaccg
     9421 tcttttgagt aaaagaattt gcttctggca agtttagcta catactcatc atctttcagc
     9481 aatgaaacat cttggtgcag ctgatcaacg tctgcgttga cttctttcat ttcagtattt
     9541 gcttcgactc tcaattcgtt caattgatgc atcctttgca tgtcatggaa aatctgcaca
     9601 cctattatcg cgaaaatgac caaagcacca acaaaaattg cagccagtct gcgtcttcga
     9661 aaaatcagtt gacgttgttg ttgctgaaat tcagcatact ttttctttgc atattccgta
     9721 tctagcgcag cgattttatt gctttttttc gtcacttgga ttcgctccaa tctttttgat
     9781 tgtctttttc attataccaa tatcgtgaaa gcttgtctaa tctatttctc tagtgtttct
     9841 gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat cttctttttt ggttgattca
     9901 tgtaattccg taattttcac ttccaacgtt ttgttgccaa actggacttt caaaatatcg
     9961 cctactttga catcactgga agatttcgcc aatttaccgt tgatttgaat tcttccttta
    10021 tcagctactt cttttgctac tgatcgtctt ttgatgattc gggaaatttt taaaaattta
    10081 tctaatcgca tatcttattc ctcacttttt aattttttta tttaatcgca ggattttctt
    10141 accaaaaggc aacattagcc attctcgtac tgtaaacaga cgaagacgaa tcactgcata
    10201 aatgaacaca gaagccccga tcccaactcc tatcacgctg acaaaaaaag cagtggaacg
    10261 atgagttaca ggaccaaata ctatattcaa tacaccataa tacagcagca gtactactgt
    10321 catacaaaga aggctgaaca tcaacttccg tccaaaatgc cgttctttcc aaaaagcatt
    10381 tagctctttt gtttcaattc gaacaaaata ccaaagcgtc acacctaatc ctaaaagggt
    10441 agacaggctt gcacctgctg ttcctaataa accggtcaga aaagaagtag tcaatccttt
    10501 taccaatagt ccccatcctg cgccttttaa agaagggcga aacgagttct tgctttgagc
    10561 aatactttga taagcttgga tgactgcagt gaaagcaatg gaaaaaacaa ataatacaag
    10621 cgctgcattt cccgcataat ctttaaacaa cgcataattg atataaggga gcagcaaggc
    10681 taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat ctaagataga ttttggctgt
    10741 ctgtaaaaac tgacgataga tccggttggt caaatgacga gtcaacgcag gcaggaatgt
    10801 cgcacttaat gcagtggcaa tcactaaacc taattgtact aaaggctgac ctcgatcata
    10861 gacccctttt gctatcttcg caccgtattc tgacagtccg taaacttcca aagcattttt
    10921 tacaaagaaa gagtcgatca gctggaaaaa gataagcaat ccactataaa tcgaaaccag
    10981 tccgccttct accagaaaac gccggatcaa gggctttgac ggctgtttga acaatggaaa
    11041 ccgtcggaag cttaacgctc ctccatgaat cttttgttca tagtagtgaa ggatcccata
    11101 tgcgcataaa ccgccaaaaa cagctccact cattgctgcc gtccctgttt ggtacaccgt
    11161 ccatccaagc tttctaaaag caaacgcact acagacaatc acaaaaacac gaacgaactg
    11221 ctcaactact tgagaaacag cagttggttc catcaagaat cttccttgga aattccctcg
    11281 ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa actacttgga tcagcggttt
    11341 caattgtatg tctcccatca tccaagcaat ccagctgcta aatgcaaaga caaacaacca
    11401 caaaaagaca cctgtccaaa atactaaggg ataaagattt tgcaaagctt gctttcgctc
    11461 gaaaggattc tttctttctg caacgtactt tgatatgaac tgcggaagac cggataaagc
    11521 taaagtcatt gctatcccat aaattggata aacttgctga taaacataaa aaccttcgtc
    11581 gccgactaag ttttggaggg gaattcggta aagggcactt aatacttttg ctatgaaaga
    11641 agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc atttctttat gatccaaatc
    11701 attgctcctt cctacgctga cagatatttt tgctcacgca acgctttgac aaacttttgg
    11761 atttcctgaa gccatactgc ttcttccatt tggttgggga tcgtcaagcg tatcaccatc
    11821 tgttcttttt caacgcctag actggctttt agttttgtcg ctgtcagtgc ttcgaataac
    11881 tgttccacac tatagcgttt tgttccaact ttacttagag taaagactat atgcgcctcc
    11941 tgtttcctta tcgtttctag gagcgctcga tcgccatcca ttttgatctg cccaattgtc
    12001 aagagatgtg ctacttcttc tggatattct ccaaaacggt cgagcagatc atcttctagt
    12061 tcatctagca tttcttgcga atcaagttca cgaattcgtt tatagatctc gattttctgc
    12121 cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga caccgaggtc gatctcaaca
    12181 gtggtcttct ccacttgact attctttcct tgtttacgag aaacagcttc gcttagcatt
    12241 tgagaataca tatcgaaacc gactgcatca ataaatccat gctgctgagc acctaagaga
    12301 ttacctgcac cgcgaatgga aagatcccgc atcgcaatct tgaatccaga tcccagttct
    12361 gtaaaatctt tgattgcctg aagacgtttt tcactgactt cgttaaggat cttctgctgc
    12421 tcatacataa agtaagcata tgcgacacgg ctgcttcttc ctacccgccc tcgcaattgg
    12481 tataacgtgg ataatcccat ataatcagcg ttctcaacga acaacgtatt tgcgttcggg
    12541 atatctactc ctgtttcaat gatggttgta gtgacaagaa catcatattg accttcaata
    12601 aattcaaaaa gcgtattttc taattgcgcc tctgtcattt gcccatgagc ataaccgatc
    12661 cgtgcttccg gaacgagttc ttggatttct tctacttttt gttcgatcgt ttctacccga
    12721 ttatataaat agaatacttg tccacctcgt cccatttctc gatggattgc ttcgcgaata
    12781 gccccaggat ttttttccat aacatacgtt tggatcggat aacgattcgc aggcggtgtt
    12841 tcgattactg aaagatcccg cacgcctaac atcgacatat gcaaagttct cggaatcggt
    12901 gttgcagtca gagtcaaaac atccacttgt gcacgtaact gtttcaatcg ttccttatgt
    12961 tttacaccaa aacgttgttc ttcgtcaatg atcagtagac ccaaatcgct aaattcaata
    13021 tcttttgaca aaattcggtg ggttcctaca acaatatcaa cttgacctgt acgcagttgt
    13081 tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac ttaatagccc gatatttacg
    13141 gggaaacctt caaatctttc cagcatcgtt tcatagtgct gctgtgccaa gatcgttgtt
    13201 ggaactaaaa aggctacttg tttgctttcc tttatcgctt taaaggccgc tcgaagcgct
    13261 acttctgttt tcccataacc aacatcacct acgagtagtc gatccattgg tttttctttt
    13321 tccatatccc gtttgatctc cgctgcactt ctcaactggt catcagtttc agagtagggg
    13381 aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc caaaagcata tcctttttct
    13441 gactctcttg cggcatataa tttgattaga tcgtcagcga tatcctcgat ttttgaagag
    13501 acttttcgct tggttttcgt ccattcactt ccgcctagct tattgatgcg aggcgctttg
    13561 gcttcagagg cgacatattt ttggatcaga ttcagttgag taacaggaat aaacagtttg
    13621 tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa cgccatctac ttctagcgtt
    13681 tccatcccaa tgtatttccc gataccatga ttggcatgga caacgtaatc tcctgctttt
    13741 aactcattat agcttttcaa tcgctctgcg tttgttactg tttgacgtct cgtttgtttt
    13801 ttcgtcgttt tttggaaaat ttctttttct gtgatagcta ctattttttc ttgaggaagt
    13861 tcaaaaccag cctgcaaagc tccttcaact aattgtacct gtcctctgat caatgcattt
    13921 ttttctgttt ctactactaa tatgtcttca tctcgaagca tttgttctgc tttgcgtatt
    13981 ctgtcctttg tcggaataaa gatcacaaca gtctgttttt gcttgcgcca acggtccatt
    14041 tctgttttca gcaatggcat ttgtccgaag aactgttgca tcgtccgata ttgaaaatta
    14101 tggattgctt ggaaacgaag atttcccatc cctttttgga agagtgagaa aaaggttgtg
    14161 gtgaactttt ccttctgcac gattttttgt atatctgctg aaaatgtctg ctcagaaaac
    14221 acccgcaatt cgctgatttt ttgagtctgc cattctgccg cttccctttc gatttcccga
    14281 tttgtttcca tgattcgctg ataatcatca acgactagta aactatttga agggaaataa
    14341 tccaagatcg tcacagattc ttggtaaaga agatctgagt agaattttgc ttcttctgtc
    14401 gggacacctg cttgccagct tgaagtcagc tgaccgaagt aatcttgcaa aaactcgcgg
    14461 tctgcttctt ctgtggtcgc agcaagccgt ttttctagca ttttttgcat ttgctctatt
    14521 ccatgttcta gatcctctgg tccgtagact tgttcactcg ttggggaaac ccagatttct
    14581 tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat accgcataga atctatctct
    14641 acatcaaata gttctacacg aacaggatag gttgtcgtta acgggtaaat atcgataatg
    14701 cttcctcgaa tactgaattc tcccggtttt gcgaccatct cttctcgtac atagcccatc
    14761 aaagtcaatt ggcgaggaag attttccacg tctatctcat caccaatatg ccaattcaat
    14821 tgcgcctgta gccaagtttt cttattcggc agtcgtttgt gcaaagatgc gactggcaac
    14881 aaatagatac ctgctttttt ttcttgaagt gcgttaagcg ttgcaactct ctcgcctcgc
    14941 gcttctggag aagaaaaggc catttcagca gaaagcactt catccactgg aaataaatgg
    15001 atctgatctt gcggaatcac atgttgcaga tcttctgcta actgattcgc ataatacaaa
    15061 tttggcatca gaataatcat gtttttattt gttttcttaa acgctcgagc aaatagaagt
    15121 gttttcgctg agcccgctaa tcctgtgatt agttgtctag gcattttttc ttctaaattg
    15181 ctgacccatt cttttacttg cggatctttt ccgactaatt gaataatatc cattcctttt
    15241 cctccttcct caattgaatc gattcatcgc atccacgaac gaatgccctt ctatgagata
    15301 ttcgactgct ttaacagatt gacacatgct ttcttctatc aaaggctgat tttctttgct
    15361 gaaaggacta agcacatgct ggactactgt ttttttccct tctggcctgc cgattcccac
    15421 cttgattcga tcaaagacat tcgtattcaa atgggaaata atacttttga taccgttatg
    15481 cccgccggca cttccctttt gacggagccg gatttttccc actgccagat ccaaatcatc
    15541 gtagattacg actaattcct ctggataaat cccaaaataa gtcattagtg ggccgactgc
    15601 acgaccagat tcattcatga atgtttgtgg tttgactaaa aggatctttt ctccattatg
    15661 aaaaaattcg gcaacttctg cttcgaacgg attttttttg aaagtagctt gatgctcgcg
    15721 tgcaatttta tccacgacca taaagccgat attgtgtttt gtatattggt atttggtacc
    15781 tgggtttcct aacccgacga tcattttcat ttttgttttc cacctaattc taaaaagcag
    15841 cacaaaaggc tggactgcta attagcggtc cagtccatcg agcttttttc ttttttatac
    15901 ttgtcaatgc atagtataac acaaaacaag gcgtttcggc taaaagagaa tcgttaataa
    15961 atgataaata aattaaaaat ttcttcaaag tttaatgaaa taattgatat ataaaaatag
    16021 tgaaataacg cacatgtaag cttttacata taatacaacc ttgttatctg ttatatttta
    16081 tagccaaata gcgtgacatg gggcttgaga aatgttagca taagcatggg aaaaaacaaa
    16141 tcaatggaag gattgatact aaatgactgc agctgcagaa aaaaaagatc atcaaaaagt
    16201 tattcttgtt ggagacggcg ccgtaggttc tagctatgcc tttgctctag ttactcaaaa
    16261 tatcgctcaa gaagtaggta ttgtggatat caatactgct aaaactgaag gagacgcaat
    16321 tgacttgtct cacgctttgg catttacttc acctaaaaaa atctattctg ctacttacgc
    16381 agacgcacat gatgctgact tagttgttat tacagctggc gcaccacaaa aaccaggtga
    16441 aactcgttta gacttagtaa acaaaaacct aaaaatcaac cgcgatgttg tcacacaaat
    16501 cgttgaatca ggatttaacg gtatcttttt agttgccgct aacccagtag atattttgac
    16561 ttactcaact tggaaattct caggtttccc taaagaacgc gtaatcggtt caggtacctc
    16621 tcttgactca gctcgtttcc gtcaagcact tgcggaatta gttgacgttg atgcacgtaa
    16681 cgtccatgct tacatcttag gtgaacacgg agattcagaa ttcccagttt ggtcacatgc
    16741 aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac aacccagaaa tcgatgaaga
    16801 agctatggtt aacctattct ttggcgtacg tgacgctgct tacacaatta tcgagaaaaa
    16861 aggcgctact ttctatggta tcgcagtagc attagcacgt atcactcgtg ccatcttaga
    16921 tgatgaaaat gctgttcttc ctttatcagt attcatgaat ggcgaatatg gattgaacga
    16981 catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt atccaaaaag taatcgaaat
    17041 tcctttaagt gacagcgagc aagaccgtat ggctgcttct gctaagcaat taaaagaaat
    17101 tctagatgaa gcttttgcta aactagacgc tgaataatct ttgaacatga cggaaaacat
    17161 ctattcggtg ttttccgttt ttttatttta tcacccttat ggtttctaaa ttttttcata
    17221 aagtagagat aaccttcgtc ttttcctcta aattatgcta tcatgagacc gttccaccct
    17281 aaacttttac ttaaggagtt gtcgatatga caagatcttc gcatcgaaaa aaatcgaaac
    17341 atgttgcttt gctttcagtt cttggtgttc tcttagtgct gatcagcttt tatacctatc
    17401 gcagtacata ctacacaaag cattttttgc cgaatacaga aataaatgga ataaatgtca
    17461 gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga agcttattct gataaaaaac
    17521 tggctattaa agaagatgga aaagtctggc aggaagtacc aaaatccgag ctcggctaca
    17581 aagatgattt cacttcggag ctttctccga tcctaaatga acaaaacgga tggacttggg
    17641 gaatgactta tgtctcagcg gcagaaaaac aagaaataga tcctgtcagt caaaaccacc
    17701 aaaaactaga cactaccgtt cagacattga caactaagct gactgatttg aacaaagacc
    17761 ggacacctac tactgatgca acgatcgaaa aagcaggtga ctctttcgtt atcaagcctg
    17821 aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa acaattaaaa agtgcggtaa
    17881 actctggtaa agatacaatt gaattgactg aattcaaaga aaaaccaaaa gtaacatctg
    17941 aagacagctc actgaaagaa caactcgcat ccatgaatgc tatcgcaaat gtaaaagcta
    18001 cttacagtat aaacggtgag actttccaga tcccatcaag tgatattatg agttggctga
    18061 cctacaatga tggaaaagtc gatttagata cagaacaagt acgtcaatat gtaacagatt
    18121 taggcacaaa gtataacaca agtacgaatg acacaaaatt taaaagtaca aaaagaggag
    18181 aagttacggt cccagttggc acttacagtt ggacgatcca aacagacagt gagacagaag
    18241 cattgaaaaa agcgattctg gcaggccaag attttactcg ttcacccatt gtgcaaggtg
    18301 gcacaaccgc tgatcatcca ttgatcgaag atacgtatat cgaagttgat ctggagaacc
    18361 agcatatgtg gtattacaaa gacggaaaag tggcccttga aacagatatc gtttccggaa
    18421 agccaactac acctacacct gctggagtct tctatgtttg gaataaagaa gaagatgcaa
    18481 ctttaaaagg aacaaatgac gatggaacac cttacgagag tccggtaaac tactggatgc
    18541 cgattgactg gacaggtgtt ggtatccacg attcagactg gcaacctgaa tacggtggag
    18601 acttatggaa aacccgcggt tcacacggct gcatcaacac cccaccaagt gtaatgaagg
    18661 aattattcgg gatggtcgaa aaaggaacgc ctgtattggt cttctaacaa aaataatata
    18721 taaaaagaaa gcacccgtac gattggtaca ggtgctttct ttttattatc taacaatgtg
    18781 ctacttacat ttcagcagca gtaaagactt ctgaaacatc atcatcgtct tctagcttat
    18841 ctactagacg ttccaattgc tctttttgtt cctcatttaa atcaatcgtc gtttgtggaa
    18901 tcatcgtcaa ttctgcttgc gccaaagtga actctttttc caactcatct cgtacagcag
    18961 caaaatcttc tggtgcagta taaatctcga atacttcagg agaagtttgc agatcttctg
    19021 cccccgcctc taatacatcc tcaaacatcg tctcttcatc aacagtcaac ccatcgcgtt
    19081 caatagcgat atatcctttg cggtcaaaca agtaactgac tgaacccgtt tctcccagtg
    19141 atccaccgtt tcgagtaaaa gctactcgga catttgtcgc tgtccgatta cggttatctg
    19201 tcaatgcatg gactaagatt cctactccac ccggaccata tccttcatac gtgatttcat
    19261 cgtaattcgt ttcatctacg gcactcgtag cctttttaat cgcccgttcc acattgtcgt
    19321 taggcatatt tgcagctttt gctttgtcca caacaagccg caaagctgga ttcatagatg
    19381 gatcaggacc gcctgcttta gctgccatat aaatttcacg agaaagtttc tgaaagactt
    19441 tccctcgttt tgcatcttgg gcattttttc ttccttggat attatgccat ttactatgtc
    19501 ctgacatgat ttttcctccc ttgttctttt gagaatacac ttctattcta gtactatttc
    19561 acaaaaaaac aaagccacag ccaatctata aacgcttctt gaatgccgca aaaagcacac
    19621 ataatatcac agcagtcaga gcaccgatcg aatccaacat gacatcttgg aacataggtg
    19681 ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc tgcgtaccct gttgctgaaa
    19741 gccaagcaag gatcgctgtc agccaccaaa tttttagctt ggggaaaaca cctaaaaaca
    19801 agctgcctcc caaaataaaa taggtaccga aatgtgcgcc cttccttaaa aagaactcta
    19861 aaaatttggc gtagcccaga tggtcgatac tcaccatact tccaccataa ttaaaacgta
    19921 tacccgctaa tgtgtcttta aatggctgat taggcagcca gctttctaat cgcgaaactt
    19981 gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa gattataacg actatcagtg
    20041 cgattactaa ataaatattt ccgtttttca tttgtttttt tgaagaattc ataagtcacc
    20101 tctgtatttt atcttagact ttttttgaag aaacaacaat cattttcata gagacttctt
    20161 ccataaaaaa gtgctatact taattttgta ttacaatatg aagggagttt tttcatgagt
    20221 tattcattaa attggaatct agattctatt ttttccggag gcagtcattc tgatgctttg
    20281 aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt attatcaccg cgtaacaaaa
    20341 tggtcaccta gttctgacaa tgcagagcag ttaaacgcta ttcttcaatt gcaagaaacg
    20401 attacgaatg gttttaccca atgcagcagc tatattactg cattgctttc tgccaatgtc
    20461 aatgattcag atgcaaaggt cttatcaggc aagctttacg caatgctgcc tcgtctgcaa
    20521 tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat ctgacaatga ttggaatcag
    20581 atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt taaacgagat tcgtcgtgat
    20641 ggctcacaat tattatcaga agctgaagaa aacattataa atacgctttc tttagatggt
    20701 ctaaatgctt ggagcagtca ttatgatacg attgtagcca gcatttcgat tcccttcgaa
    20761 caagatggac aagtagtcga attatctgct ggacaagcct tcaataagat gatgggcgat
    20821 ccagatccaa aagtcagaga aacacttttc gcagcatggg aagaagcctg gaaagaaaag
    20881 gcacccctct tcacggatac tttgaatcat ttagatggtt ttagactttc tgactatgaa
    20941 cttcatggtg tcacagattt tctacaaaaa ccattagagt acaatcggtt gaagaaagaa
    21001 acattgtctg tcatgtggga tacaatccag aaaaacaaac aaccccttgt taattacttg
    21061 actagaaaag ctaatctttt cggtaaagaa aaaatggaat ggcaagatca agatgcacct
    21121 attattttag gcgatttgaa agaaaaaacc tttagttttg acgaagcagc agcatttatt
    21181 atcgaaaact ttaacaaatt cagtccaaaa atggctaaat ttgcccaatc tgcttttgaa
    21241 aagagctgga tcgaagcaga agatcgtcct gggaaacgtc ctggtggtta ttgtactgag
    21301 ttaccagaaa caaaagaatc aaggatcttt atgacctaca gtaattctgt taatgaggtt
    21361 gcgacacttg ctcatgaact aggacatgct ttccatagca gcacgatgtg ggatttacct
    21421 tctttaaatc gcgaatatgc catgaatgtc gcagaaacag caagtacatt cgcagaactg
    21481 atcgtagccg atgctacatt aaaagtcgca aaaacaaaag aagaaaaaat caatttgcta
    21541 gatacaaaac tgcaaaatgc tttagctatg ttcatgaata tccattcccg attcattttt
    21601 gaaaatcgat tctatgaagc acggcaagaa ggattagtct cagaagataa gatcacagag
    21661 atgatggttg aggcgcaaaa agaaggctat caaggagctt tagctactta ccatccatat
    21721 ttctgggctg caaaattgca tttctttatt gatgacgtac cgttttataa cttcccatac
    21781 acatttggct acctctttag tttgggcatc tatgcttacg ccaaccaaaa gggagcaaat
    21841 tttgaagaag aatatatcca tttactacgc gatactgcct caatgacgac ggaagatctt
    21901 gcgaagaaac atttgaatgt cgatcttacc caaccagatt tttggcaagc aggtatcgac
    21961 atggtgactg aagatatcca aacgttcatg gaattgacag aagaatttat ctgatttcta
    22021 ttttatcaca ataaaaaaag agttagtcca gaatgggcta actctttttg attaatcatt
    22081 cttacatgct gacagcaaag tcaggggcaa accattgaac tgaaccaact tttacgcttt
    22141 cgcctggttg aggagcatgg atatattgtc ctccgcctaa agcaatcgct acgtggtaag
    22201 ttccgccttg tgacccccag aataacaagt ctccagcttt tgcttgagaa actgaaattt
    22261 ttgttccagc tgattcttga ggaactgtcc aaccaccgat gtcacgacca gttacttgca
    22321 tgtaaacata gcgtgtgaac cctgagcagt caaagccact tggatcttta ccgccccaaa
    22381 catatggagt accaatatat ttgtaagctt ctgctacgat tgctgcacca ttcacacttc
    22441 cagaaggagc tggagcaggt gttacaggtt ttggttgttc aggtgttggt gttggcgttg
    22501 gagccggagt actacttcct gtgccgtttc ctgtatcaac actttgatct gttgatggtg
    22561 ttgtaggcgc aggtgttgtt gattcttctg ttgttgaaga ttcaggcact gttgttgatt
    22621 cttctgttgc tgaagattca ggcactgttg ttgattcttc tgttgctgaa gattcaggcg
    22681 ctgttgttga ttcttctgtt gctgaagatt caggcgctgt tgattcttct gttgaagatt
    22741 caggtgttgt tgattcttct gttgctgaag attgtgtagc tgatgatgat tcttcagtca
    22801 ttgaagattg tgtagctgat gattcttctg ttgctgagct ttgtgcagta gatgaagctt
    22861 gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc tttttcagct ttttcttgtg
    22921 cagcttgttg acgagcttgt tctgctagac gttgttgttc acgaatacga gcttgctctg
    22981 cttcagcttc tgctttttga cggttcaagt cagctttctt gtcttcagca gttgcttgtt
    23041 cagcagctaa tgaagttttc agtacgttca aatcagcttg tttagataaa agatcgcctt
    23101 tttgagattc caaagcagct tggttttcag ctaattcttt tagtttagca tcgttttcag
    23161 cttttttgtc ttctacagct tttttatctt gtttttgttg ttccatcaag tcgttgtttg
    23221 cttttaccat cgttgtcatt gcttgaacgc gtccaatagc atctgctaat gaatcagcat
    23281 tcaatacagc atcgatgtag tttgagcttg tgttgcttac ttgtgcttcg cgtgcttgtt
    23341 tttggattgt atcttcacgt ttttcaatac gttcttgtaa atctgcaatg tcttttacta
    23401 attgtgcaga ttcttgacgc aatgtatctt gttttgctaa taggtcttgt gcttgtgtgt
    23461 tgatttcaga aacttggctt tctaaggcat cgatttgtga ttgtgcatct gcttgttgat
    23521 ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg agaatcaaaa tcgtccgccg
    23581 cggcagcgat cggtgatgct acggcagtca aggtcattga acataccatt actgctgata
    23641 ttaaactctt tttcactctt cattcctccg actggcttaa tttaataaat atctaaatat
    23701 atttaagtct ttcttaacga tataaaaatt gtaccatagc agtgtgtcaa cgatataata
    23761 gttatgttac aaaaatgttt caaaacaact tttaaaaatg acaaaactgt tcgtttcaga
    23821 aaatgatcat gaaaaagccg cgttaagtct agcatttctt aagataacaa cgattctttt
    23881 tcgctttcga cctaaaaata aaaaaagaaa ttattcatca ctgaataatt tcttaaaggg
    23941 aaaaatgaat aatacaaata taatcatgtt caacagcagt gtaggtccta aaaaccttgt
    24001 aataaaatac gtgttattta ctactgctaa tttaaaaatc aactggacca ccatcgtaaa
    24061 caattcataa cccgtgacaa agatgatcat actgaaaaat tcagtaaaga tgttgacatg
    24121 gataacgtct ttcattacat acatcaacca tacaattaaa gggagagcta ctgcatagat
    24181 accgataacg ccaatatagt aagcgtcata gatagccccg agaacaatcg tggtaatcag
    24241 taaataacgt ttttcaaacg ccatactgca acataataat gccaatatca gaaagtgggc
    24301 atttgacata taggtgcctt tcgaccactc ccctagcatt cttgttaaat gaccatctat
    24361 caacatcaaa aagaacaaaa cgattggcag atagtacttc attgtttctt tcttcaacat
    24421 ggactaatcc tccgctagcc gttggacaat cgtcacaact gacacgtcat acatttctgc
    24481 ataaggtttc acataaactt cccgatccag cccataacta tctggtttcg ttttaataac
    24541 cgtaccgatc ggcaagttag ctggagaatt ccctccaaga ccggatgtct ggacaacatc
    24601 cccttctttg atatccgtat caccagttaa ttgggtcacc actaaggcat ggagcttttc
    24661 atcatagttt ttaagcaaac caaacgcttc gccattagcc gaagataccc gtactggaaa
    24721 atgattggag ctttcattag aggatgacag taattcgatt ttagacgaag ccgtattgac
    24781 ctcgatcact cggccaatca atcctttttg cgacatgact gccatatttg cttcgatacc
    24841 gtctttagaa cccttgtcta cgatcagcat gtcctgccaa ctgtcaggcg aacgagtaat
    24901 gacatttgcc gttacttttt cgtaactagt cagcgtctga ttcaactcaa gttctttttt
    24961 caacgcttcg atttctctct cgtaattctt gttttgctgt gaaacgtcat catagctatc
    25021 gattttttct tttagccgct cattttcggc ataagttgaa aaaagattat ggacagaagc
    25081 tactccattt cgtacccacc gtactggggc agagattgtt ttatcaacca ttgcaacact
    25141 gtcattaacg atggattgga agaaattggg ctttccttca ttcgctcgct gcgcagcggt
    25201 cacacttagt atcgtcacca cgatgataac aataaccaag gtaataataa tatttttatt
    25261 aggattgaac ttcttcacaa agacacctcg aattaattag actcttgtgt tcaattttat
    25321 cactaaatgc cgtgaaacaa aaggtaagtt gctcctctta agcttttttt tagcaaataa
    25381 tagattctgt catttttagc ctgaaagaca aattttttta tttgtttatg gatcaatctt
    25441 tgctttgaat aactgcaatc ataccatctg aaaaagaaaa ttctgcggta tctgaaagaa
    25501 attcgttact tgcataagat gtcggatatt ctacttcttt attttccaat acatacttgc
    25561 tgccttttaa tgtaagattc gagacgggag tcaagcagca ataagctaaa tacttcatat
    25621 ctggtatctg tcttactata tgctctccag gcaaataata agacaaataa ttttgacaat
    25681 ctctgataat aagctgatgg gcaaagggtt tgaagcgagg ttccatacct aaccagagat
    25741 tggctaaaag atgatccaat cttccgcccg tagctccaat caaagtgact tccgcttgcg
    25801 ggtaacgttc aaatgttttt aagataccta actgtgtatc tgtatcgtct ttttctgcag
    25861 gggaacgggt atactctttg gcttttgcaa atacttcctt ctgctcttcg ttcgacaatg
    25921 aatcaaaatc acccactgcc agatctactg ggtagcctgc ttgaagcagg tacagcgcac
    25981 cacggtctat cccgacatag gcatcgtatt tggacagcag agactgcggc cagttttgcg
    26041 gattacctcc agcagctaat aaaattttca tttagtctaa tgcctctttt aatcgttcga
    26101 tttgtccgac tggattttcg gctccataga tatatgatcc tgcaacgaag acatccgccc
    26161 ctgcatcttt acagattttt gctgtttctg gaacgattcc gccatctact tcgattgcat
    26221 aagtccaatt gttctctcta cgtagatcag cgagttccgt aattttttct actgtttctt
    26281 caataaagct ttgtccgccg aacccaggat tgacagtcat gactagtact tgatcagcca
    26341 aaggcaaaac atgtttgatc atggagactg gtgttcctgg attgataact actccagctt
    26401 taactcctaa gctttttgtc atttgaagtg cccgatgaat atgaggagtt gcttcgacgt
    26461 ggacagtaat gatatctgct ccagcttttg caaatgctgg aatgtagttt tctggatcaa
    26521 cgatcatcag atggacgtct aacggtaatt tagtaacagg acggattgcc tgaacaatat
    26581 ttggccctaa tgtgatattt gggacaaatt ggccatccat tacatctacg tggatgtaat
    26641 ctgcgcctcc tttttccact aactcaatgt ctctttgtaa attggcaaaa tctgcactca
    26701 aaatggatgg tgctatcttc attcttgttt cctcctattt tcgtttacct tctttttttg
    26761 catacattgg ttttcttttt tcgatttctt gtaagaattg caaataattg tcgtaacgtg
    26821 tctgtgcgat accacctgtt tctactctat gttttacttc gcagccaggt tctttagcat
    26881 gcatgcactc tctaaatttg caatggacag aagcttctac aaattcagga aattgtctcg
    26941 gcagttctcc tgcttcaatt gtcaaaaagt caatcgagct aaatcctggt gtatctgcta
    27001 ctaacccgcc atagatagga atcagttcca catgacgtgt tgtatgccgt cctcttccaa
    27061 gtgcatcaga aatctctcct gttgctaaat tcagttccgg tgatatttga ttcagtagcg
    27121 tcgattttcc tgctccagac tgacccataa agaccgatag ccgttctggg aagtaacgga
    27181 tcagttctgt cgtatcaccc gtatgattgg gtacaatcac cggatagcct attttagtat
    27241 aggtttgctt gatctcttcg attttttctg ggtcgtctga caagtcggtc tttgtcaaaa
    27301 aaataatcgg ttcgatcgac tcgtattcca aggtaactag aaagcggtca agtagattat
    27361 aagaaaatgc aggctctacg atacttgtga caatgattcc aagatcaaca tttgtgactg
    27421 gtgggcgaac taattgattt tttctcggca agatttccag aagatagccg tctgtttgat
    27481 tttcactctc aaagatcacc tgatctccga caagcggcgt gattttccga ttccggaaat
    27541 ttcctcttcc tcttgtttga taggtctctc cgtttgcgta tacatagtaa aaaccgctga
    27601 ttgcttttct gatttgtcct ttgataagtg ccacctccgt ttttgtttat tttaccatac
    27661 gaagaggaaa gtaggtgtta ttccatgcaa acttctcaaa gtatatggct tgaaaattga
    27721 tcaaaagaga tagaaacaca aaaaaga
//
LOCUS       DACDIH010000030        26874 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDIH010000030 DACDIH010000000
VERSION     DACDIH010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.030"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1088)
                     /locus_tag="IV281_001412"
     CDS             complement(<1..1088)
                     /locus_tag="IV281_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8780410.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKE"
     gene            1298..2299
                     /gene="ccpA"
                     /locus_tag="IV281_001413"
     CDS             1298..2299
                     /gene="ccpA"
                     /locus_tag="IV281_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP8780411.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(2636..4981)
                     /locus_tag="IV281_001414"
     CDS             complement(2636..4981)
                     /locus_tag="IV281_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8780412.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            5260..6162
                     /locus_tag="IV281_001415"
     CDS             5260..6162
                     /locus_tag="IV281_001415"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP8780413.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            6375..8150
                     /locus_tag="IV281_001416"
     CDS             6375..8150
                     /locus_tag="IV281_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP8780414.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(8216..9802)
                     /locus_tag="IV281_001417"
     CDS             complement(8216..9802)
                     /locus_tag="IV281_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP8780415.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            10053..10277
                     /locus_tag="IV281_001418"
     CDS             10053..10277
                     /locus_tag="IV281_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP8780416.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            10419..12170
                     /locus_tag="IV281_001419"
     CDS             10419..12170
                     /locus_tag="IV281_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8780417.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            12170..13954
                     /locus_tag="IV281_001420"
     CDS             12170..13954
                     /locus_tag="IV281_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8780418.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            14280..15131
                     /locus_tag="IV281_001421"
     CDS             14280..15131
                     /locus_tag="IV281_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8780419.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            15185..16936
                     /locus_tag="IV281_001422"
     CDS             15185..16936
                     /locus_tag="IV281_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8780420.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            16940..17110
                     /locus_tag="IV281_001423"
     CDS             16940..17110
                     /locus_tag="IV281_001423"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780421.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(17150..18052)
                     /locus_tag="IV281_001424"
     CDS             complement(17150..18052)
                     /locus_tag="IV281_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8780422.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            18283..19686
                     /locus_tag="IV281_001425"
     CDS             18283..19686
                     /locus_tag="IV281_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8780423.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            19686..21119
                     /gene="ascB"
                     /locus_tag="IV281_001426"
     CDS             19686..21119
                     /gene="ascB"
                     /locus_tag="IV281_001426"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8780424.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            21264..22190
                     /gene="trxB"
                     /locus_tag="IV281_001427"
     CDS             21264..22190
                     /gene="trxB"
                     /locus_tag="IV281_001427"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP8780425.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            22404..24131
                     /locus_tag="IV281_001428"
     CDS             22404..24131
                     /locus_tag="IV281_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP8780426.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            24203..24496
                     /locus_tag="IV281_001429"
     CDS             24203..24496
                     /locus_tag="IV281_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8780427.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(24588..25448)
                     /locus_tag="IV281_001430"
     CDS             complement(24588..25448)
                     /locus_tag="IV281_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP8780428.1"
                     /translation="MTSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            25540..25968
                     /locus_tag="IV281_001431"
     CDS             25540..25968
                     /locus_tag="IV281_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8780429.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            26130..26306
                     /locus_tag="IV281_001432"
     CDS             26130..26306
                     /locus_tag="IV281_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP8780430.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            26333..26779
                     /locus_tag="IV281_001433"
     CDS             26333..26779
                     /locus_tag="IV281_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP8780431.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         8554 a         4787 c         5681 g         7852 t
ORIGIN      
        1 aattcttttg gtgtagtcgt gaatggcaca cagccatcgc ttgtcacata tacacagtct
       61 tcgatacgta cccctacttt ttcaggaata tagattcctg gttcaagaga gaagcacatc
      121 ccttcttcga ttactaagtc atttccttct acaagggaag ggaattcgtg gacggttgtt
      181 ccgatcccgt gacctaaacg gtgattgaaa tattcgccgt agccatagct gttgatcacg
      241 tttcgtgcga tttgatcaag ttcacctgct gtcacacctg gacggacggc ttcttgtgcg
      301 gcaagctgtg cttcaagagt gatgttgtag attttttctt ggaagtcggt tggtttttga
      361 taagcaattg tacgagtagc atcactgcaa tagccattcc aaacaacgcc aagatcgaat
      421 aacacaagtt cattcggtga aatcttcgta tttcctggtg taccatgagg gcttgcagca
      481 tttgctcctg caaggactaa tgtgtcgaaa gacatttgtg aaacaccttg ttttttcaat
      541 tggtattcaa tttctgcaat gatcgcttgt tcagtcacgc cttctttcac tgctttaaag
      601 ccaatctcaa atgcaacatc ggcccattta ccagcttcga tcagtttgtc tttttcctct
      661 agagttttga tcagttgtag acgttggatg atggaagtta gatcagtgta agaatccgtt
      721 cccgtcaaga tatcagagat agcatcgaag cgctcgacag tcaaggcgtt cttttcaatc
      781 cctacattgc caggctgacc atagcgagca cgaagttctt ttccgatgat ctcccaaggg
      841 ttttcgctgt ctaaatagcc acggacatca tacgcccaac tactgttttg agcatcttca
      901 acttccaaag caggagtgaa aaggaaagga tcttgttcaa gagggataaa aagagctagt
      961 acacgctcat gaggatcact ttcgtatccg gaaaagtaag cgatgtggcc agggtcgctg
     1021 atataagcca attcgacttg gtgttgtgcc atccattgac gtagttcatt gatttttacg
     1081 ttattcattt ggcaacctac tttctaaatt aattttgtat tcaataattg taccactgat
     1141 ttattaaaag accaacgttc taaaaactga aaaaagtaaa aattttcatg aaaacagaga
     1201 taaaacggtt gcaattcttg agattatttg atattctatt ttagaaatgt aagacgtatt
     1261 ttcagaaaac atataaaata tgaataggag aacacaaatg gaaaagcaaa caatcactat
     1321 ctatgatgta gcgagagagg caaatgtttc gatggctact gtttcacgtg tcgtaaatgg
     1381 caatccaaac gttaaaccag caacaagaaa aaaagtcttg gaagttatcg atcgcttgga
     1441 ttatcgtcca aacgcagttg cgcgtggctt ggcaagtaag aaaacaacaa cagttggtgt
     1501 aatcatccct gatgtcagca atatgttctt tgcttcttta gcacgtggta tcgatgatgt
     1561 ggcaacaatg tacaaataca atattatctt agcaaattca gatggcaacg accaaaaaga
     1621 agtcaatgta ttgaataact tactggctaa gcaagtggac ggcgtgatct tcatgggtca
     1681 ccatatcaca gacgaaatcc gtggagaatt ttcacgttca aaaactcctg ttgtattagc
     1741 tggctctatt gatccagatg aacaagtagg tagcgtaaac attgattatg tatccgcaac
     1801 aaaagatgca gtaaacaaat tggcaaaaag cggcaataaa aaaatcgctt ttgtcagtgg
     1861 tgcgttgatc gatcccatca atggacaaaa tcgtctgaaa ggctataaag aagcattaag
     1921 cgaaaataac ctaccatata gcgaaggatt gatctttgaa gcacaataca acttcaaaga
     1981 tggtctatct ctagtcgatc ggatccataa tagcggtgca acagctgtct atgtttcaga
     2041 tgatgaatta gccatcggta ttttggatgg tttattagac cgtggcgtga aagtaccgga
     2101 agaattcgaa atcatcacaa gtaacaattc actactgaca gaagtagctc gtcctcgttt
     2161 aacaagtatc acacaaccat tatacgattt aggggctgta tctatgcgcc tattgacgaa
     2221 aatgatgaat aaagaagaag tcgaagaaaa aacgattatc ttgccttatg gaatcgaaga
     2281 aaaaggatct acaaaataat aaaaggacaa ccattcagag gttgtgtgcc tgccgtttaa
     2341 ctctgcgtta caaggagcaa tttcaaaaaa cagcatctca tgtttgatga atattgtgac
     2401 tcgtgattcg gagttggcag gagaacaccg tttatttggg aaaaggggtt atgacaaaat
     2461 tttgttataa tccttttttt gtgtcttaaa aaagagagaa agtggttgaa attacattta
     2521 tcgtatataa aacaatccgt gtatagatgc tgcattttgt tcaccaccat caaaaaaata
     2581 agggactgtg acaatactat tgtcacaatc ccttattact gtgtattcta ttaaatcaat
     2641 catctttctc atctgattct ttagttgctt gattcgctct tgcataagct gaagcggtgt
     2701 tccagtaatc tgtatagttt tcatcggtac cgccaacgcc aaatttaaag gcgcttttct
     2761 tcggtccatt tttcgcccat agcgattcaa ctgtttttcc tggtgtttgg atggattttt
     2821 tattgacagt aacttttcca ggtgtttgac cagtaaatcc agatactttt ttcttcatca
     2881 catcagatga taattcgaat ttctcatcag tgccaaaaat gtcaggtttt gcttggtaag
     2941 cttgattgat cagattggca agataattag ccgaacgtat ccttgctttg ctgtccattc
     3001 cagtattgtc atcgtgccct gcccagctac tgatggtaat agacggagta gagacaatta
     3061 gccatgaatc tcggtattca ttggtagaac cagtttttcc gacccaatct gcttttccta
     3121 gatttccgtt taagcttgag atggcatctt tgaatggtgt agtgattttt gcattgatca
     3181 cactacgcat catgtcattc atgatcgatg ccgttgcttc cgaatagatc cggacagagt
     3241 tgctttcgtg tttataaatt acatttcctg cgttatctgt gatggaatcg atgatatagc
     3301 cttcttcata gacgccgccg tttgctaaag cctgaaatcc gttggtctgt tgaagcgtcg
     3361 tgacatctac tgtacctaaa ggagcagatt caacgcccca attgtcattt gccgggtagt
     3421 tcatctttgc caagtagtgc tcgtaagcat attgtttcga tccaccggaa ttcaacacat
     3481 cttgatataa gtgataagcc gggatattgt tggaccattc tagtgactca cggacggttt
     3541 ggaaggtgtt cgatccttcg ttagtagcat tgacgatttt ctcgccagca ttttctcctt
     3601 cttgccaagt tgttgcataa tctgatacac gggattctga tccgataagc cccatgtcga
     3661 ttgcaggtcc ataaacaaga actggtttga ttgatgagcc agcttgacgt tctgtatcga
     3721 aggcatggtt attttgattc tgactgtaat tccgtccgcc aacgaatccg tagatgcggc
     3781 cagttctgtt atccatcaat acatttccgg tttctacttg actgccagca ccatcatcta
     3841 acatgtatcc ataattttca acgccatttt gcaatgcagc atagacatct ttatcgattg
     3901 tcgaatggat tgtatatcct ttattttgga tcgtttgctg tgccttttga taataagcgt
     3961 cactgaccga tgaatcagac atatcagcgt tgtctttttc agccaattgt tgtgcaatga
     4021 ttcttgttgc ttcattcatc acagtataat acaagaattc tctgtcagtc tgttctgcga
     4081 tttgttgtgg tagaaagtct tttgtaaggt cataggcttt tgcttcttcg tattgttctt
     4141 tcgtaatttg gccttcacga tacatactga ataaaacaaa atcttttctg gcaaggccgg
     4201 cagaaagatc ttcttttagc gcgcctgtgt tcgtgtatgg actatacgtg atcgggcttt
     4261 ggggtaatcc agcgatataa gcagcttgcg gcagcgtcac atcttttgca tctacaccaa
     4321 agattccttt tgcagcttcc tggactccag caatgttttg tcccttatta ttacgaccaa
     4381 atggtgagac attcaaatac gtagcaatga tctcatcttt tgaaaagtat ttttctactt
     4441 gtgcagaaag aatgatctcg tttgctttac gtttgaatgt tgtttcatct gtaaggatct
     4501 gctgtttgac taattgctgt gtcaaagttg atccaccaga agagccaata ccagtagctt
     4561 cagatactaa tgctcgtaaa acagctttag gaacgtaacc ttggtgttta tcgaagtatt
     4621 catcctctgt gctgataatt gcagttttca ataaaggtga aatcttatca gatgaaatag
     4681 tcgtgcgcat cagatctgtc tggatttttg atatctctgt attatctgca taaacaatct
     4741 ttgacgtttc tgtaatgttt cctagttctg tctgcagtgc ttttttagtc ggcaattgtg
     4801 tgtcttcgac gagataggca aaatagccag cgcctattcc aatacctaga gctgcgaaca
     4861 tcaaaagaag aacagtaata aaaacagtca atgattgaaa aactctaaaa ataatcttag
     4921 gaacaaacca tttttctttt tttcttctat tctgtttttt tcgagtattt ttgcgtggca
     4981 aaagggtcac ctcaactaat ggttttattt cgctaattat agcaaataat agaacaaaaa
     5041 aacagacaag gctgtttttg gcaggaattt cttttttaaa agagttaaaa cgtgagaaaa
     5101 tgatctttat tataaaagag ttcttaagct tttctttaga gaaaatttca gttactatgg
     5161 agaacagggc aaagaaaaat caatcatttt ctttgtatat catcttacca atatatagga
     5221 attgttagta acaattgcct ttcaaggagg aatccatcca tgttattagg ctcacacgtc
     5281 agcatgagcg gaaagaaaat gctgttagga gcagcagaag aagcagccag ttatggatct
     5341 tcgactttta tgatctatac aggcgcccct caaaacacta gaagaaaagc catcgaagag
     5401 atgaatattg atgccggaaa agcgtacatg gcagaaaaag gtctaacaaa tatcgtcgtt
     5461 catgcccctt atatcattaa tttggggaat acgatcaaac cagaaatgtt tccttttgcc
     5521 gtccaatttc tgagagaaga gattgttcgt gcggaagctt taggcgctac tcaaattaca
     5581 cttcacccag gtgctcatgt aggggcagga acagaagccg gaattgctag aattattgaa
     5641 ggcttgaatg aagttttgac aaaagaacag acggcacaaa tcgctttgga gacaatggca
     5701 ggtaaaggta cggaaatcgg tcgaacattt gaggaattag cgcaaatcat cgatggagta
     5761 aaattgaatg aaaaattatc cgttactttt gacacctgtc atacgaatga tgccggttat
     5821 aatattaaag aagacttttc aggtgtaatg gaagagtttg ataagatcat cgggttagat
     5881 cggcttaaag tactccatat caatgattct aaaaatccgc aaggttcgca taaagaccgg
     5941 cacgccaata ttggttttgg aacaatcgga ttcgacacgc tgaataagat cgtccatgat
     6001 ccaatgtttg acaatatttc gaaaatcttg gaaactccct atgtaggcaa agataaaaag
     6061 aaagcttacg caccatatgg aaaagaaatc gccatgttca aagcgcaaac attcgatcca
     6121 gaagtgtttc ctgagttatt agcagaagaa gtgatactct aagattttct atctgacagc
     6181 agcaaaaaag gaccaggcac attgccttgt ccttttttgt ttaagaaaca aaggagtact
     6241 ttttgcagtc tttttcatca tgcatcttct cccccctctt tttaaattta gacaaacaag
     6301 gtatactggt agaaaagtaa aaaaacgaaa agtttcacaa gcaagtggca aaacgtgatt
     6361 ggaaggacga aaagatgctg aacactttaa aacccttgat caaccaacta aaaaagaaac
     6421 atacatactt tattcttggc atagccctat tatttacaat tattcgtttg tatttatata
     6481 gtaaagcaat ctatggaaca gacatgtcgg caacctatga tgaccagctg ctgattcgct
     6541 atgctcaaaa tattttggac ggtaaatggc taggggagta tacgactaca acactttcca
     6601 aaggaatctc ctacagctta tttttagtac ttgcacataa actgcatctt tcttatgcag
     6661 ttttgttcgg catgttcagt atttttgcca gtatattggt tgcactcgcg tttaaaccga
     6721 ttgtgaagaa caagtatctt cttttaagta tctattttta ttttttgttt tctccgatta
     6781 gttttacaca tgaatattcc actcgaacat atcgtaatgc cctcgtgatt ccggctgttg
     6841 agatcattat cgcttgttta ttagctattt attaccgcaa atacggttct agcagaaaat
     6901 tgctgccatg gtttttaggg ctttctgtcg cttttccttt cttttggttc attcgcgaag
     6961 attctatctg gcttcttcct tttactgtag tagctttggg tatcagtttc gtccagatct
     7021 tgctagggca agacttttct ttcaaagaag taaagaagtt aaaaaaacga ctctttcttc
     7081 tctcgaaacg ggaatggctg aaactttcat tgtttttact tcctatagtt ggcttttcca
     7141 tcgtctatca atggatcgaa tataaaaatg agacaacata cggtattgca accatcaatg
     7201 accgttccag tggagaattt ggacgtttga tgaaacaact gatccgaata gatgatgaaa
     7261 cgaacttgaa taaagaaaac agtgagatat gggtctcaca tgctgcacta aataaagcat
     7321 tagccgcatc tcctacattc catactgttt ctacccgtat tgacgaatta tatacgactc
     7381 acgcgtggac tcaaggtgga aaagtaaaag aattacccgg agatatcatc ttttgggcat
     7441 tgcgagatgt ggtaagcgaa tctggttatt accaaaacaa tgcaaaagaa accaatgatt
     7501 tttggaaaaa agtcaaccaa gaattagaag aagcctatca caagggaaca atcaaaaaga
     7561 aaaaagagtt ctatctgatg ggatcaggag atggaaagac gatgagtgat ctgccaatcg
     7621 tattcgattt ccttcaatca ggatacgctt atgcatggcg atataaagat ttccatcaag
     7681 gaggaaatta tagtttcgga acgcaagaac aagtcaaaca ggctcaagaa ttattaaacc
     7741 ttccgctaat ggagaattgg atcgatagta atgaaccgag aacacctaat ttccaattaa
     7801 caaaatcagc aaaagtcgcg aatgtcatca taaaaattta ccaagttctg gctattcccg
     7861 tgctgattgc ttcaatagca gggatattac ttatgatact aggaaccgta tttgcaaaag
     7921 ctgatcgtgc ctattatagt tcctctttga ttgtggtcac cggattaata ctgacacaat
     7981 tggtttttct tttcggtgtc tcatggtttt gttcttatgc gccagaaaaa aaggactatt
     8041 ttttaagtgt ctacactggt gcaggcgtgc cattaacgca attcatcgta gtaacaagca
     8101 tgctagtttt cagtaaatta aatctgaaaa aagctttatc ttctaaataa aaagaccctt
     8161 ggtagctaga tttttaacct aactatcaag ggtctttgtg tggaaaaatc agtttttatt
     8221 cgatttcttc taccgtttcc tgcaaaagct gttcatattt tttgtttcct ttatataaat
     8281 caccaatcgt ccatgccaac aggagaagga caagaacaat gattacataa gcgatgtgat
     8341 cagccaatgc tagttgagcg gaagtggctt gctcgccgaa gaaactttcg atctgtcctg
     8401 gtaatcctcg taagttcaag taagttagac taatcacaga gaaccagcca agaagtttta
     8461 cgatgaatga atttttaaat cggctaccca tctccgcttg gctgttggtc atcatcagta
     8521 aaggaatcat cgaaaacggc aacgcaaaag ccaagaacac ctgggattca ttcattaagt
     8581 tattcaaagc ggcatgttct tcgatcgttc cttttgtact tgtaaacaaa acacaaatca
     8641 atacaggaat cacagaaagc aatcgtgtga ccagtcttct tagccaaatc gggatacgca
     8701 tatggatgaa tccttccatg atgacttgac cggtcaacgt accagtaata gtagagttct
     8761 ggccagaagc aagaagtgct acagcaaata aagtggacaa gataccagat tttgctacag
     8821 caatcaaaat accattgctc atagctgatg gatcagataa agcttcatac agaccaaaaa
     8881 aggaaggatc ttggacagaa ccggttttga agacagcgac acccatgatc aatagcagtg
     8941 cattgactag aaaagccatt gacaactgga tattagaatc ccaagtagaa aaacgtacag
     9001 cttgtgctac atcttcttcg ttttggtgat cgatcttccg agtttgtgaa actgctgaat
     9061 gaagatataa gttatgcggc atgactgttg caccaataat ccctaaagct ccagtcagcg
     9121 gagtttcgcc gccgatgacc ggtgagtctg agaatgtatg actgttaggg ataaagccag
     9181 caaataaact gtgccaatct gggttagaca agccaacttg atagacaaaa acgaataaaa
     9241 tgacgaaaat taagcagaca accagtgctt cgatcttacg aaacccgact tttgtcagta
     9301 gtaagagaac gaaaacatct aatacagtaa taaagacggc aatcaccaat ggaatatcaa
     9361 aaagtaagta gagggcgatt gctgcaccaa tgacttctgc aatatctgtt gccataatgg
     9421 ctaattcagt caagatccat aaaatgacac ctaatgtctt acttgtgcgt gctcggattg
     9481 cctgtgccaa atccatttgg ctcacgattc ctaattttgc tgccatatac tgtaaaagca
     9541 tagcaatcag actagacatc aaaataactg acatcaataa atattggaaa ttttgtcctc
     9601 cggtgatcga agtagaccag ttacccgggt ccatataccc aactgctact aacgcaccag
     9661 gtccagagta agcaagaagt gttttaaaaa aacttttccc ctttggtaca tcaactgttc
     9721 catttacttc agctaaagat aaaccatttg tatgttcaat aagtcgatgc ttatgaaatt
     9781 gtttattgga ttgattattc aagaaaaaac cacctttcct atttatctac gattacattc
     9841 tacacctcaa aaagaaaaaa ttaaagaaaa agattcggta cgcctaattt tttttcatgg
     9901 cgcgtcttgc tagatgacga aagaaatttt gttctcctct aaaaaaacca ctattttttg
     9961 catgaaacta aagaatacgc ctttcttttt tcaataaact ttagtaaaat aagtcattga
    10021 gagcaattcg gaaatagaaa ggagagattt atatgttatc aacaggtatt gtcgtactta
    10081 ttgctattat tgctttgcta atcggcgcag taggtggctt tttcctagcg cgtaaatata
    10141 tgaaagacta tttagaaaaa aatcctcccg ttaatgagga catgttgcga tcaatgatga
    10201 tgtcaatggg gcaaaaacca tctgagaaga aaattcgcca gatgatgcag caaatgaaga
    10261 accaagggaa aaaataggtt ctgacccaac gactttttac gggctgattt tttcagtccg
    10321 ttttttattt cgtaatttgt tagactattc agattattca gcaaaaaaga caagcacaga
    10381 caatcctagg ctcttgataa agacaaagga ggagtaaaat gtcgattttt agaaaattag
    10441 gctggttttt tcgtcaggaa aagaaacatt atttaattgg ggttttctcg ctattcgcag
    10501 tggccttggt acagttagtt cctccaaaag tgattgggat catcattgat gagatcgccg
    10561 ataaaaattt atcaataaag atcattttcg ggtggatctt cctattgatt atcgctgctt
    10621 ttttacaata tctttttcgt tatatctggc ggacgaatat ttggggaagt gcagctcgat
    10681 tggagaaaga acttcggcag cagttattca aacattttac agaaatggac aatctctttt
    10741 accaaaaata tcggactggg gatttgatgg ctcacgcaac gaatgatttg aatgcgatcc
    10801 agaatgtagc aggagctggt atcttgacct ttgcagattc agtgattacg ggcggaacaa
    10861 cgatcattgc gatgattttg tttattgatt ggcgcttaac actgattgcg ctgattcctc
    10921 tgcctttgct ggccgtcact tcacgagtgc tggggtcaaa actccatgat gcttttcgtg
    10981 attcgcaaga agctttttct agtatcaatg ataagacaca agaaagtatc actgggatca
    11041 aagtcattaa aacatttgga caagaaaaag aagatctagc ggattttgaa gaaaagattg
    11101 aagatgcaat tattaaaaac aaacgagtca attttttaga ctccttattt gatccattca
    11161 ttaccttgat tatcggtctg tcttatgttg caacaattat tttaggtgga aagtatgtct
    11221 tggaaggcac gatctcgatc gggcagatgg tctcatttat tagttatatc ggtatgcttg
    11281 tttggccgat gtttgcgatt ggtcgattgt tcaacgtatt ggaaagagga aatgcttctt
    11341 atgatcgcgt caatgaactt ttgcatgaaa agacgcatat cgtggaacaa ctgggtgcag
    11401 tgcagacccc ggcaaaaggc gagctgaatg tagctatttc ctcttttaat tatccgaaag
    11461 aagaatcacc agcattagaa caaatcaagt ttgcgatcca agaaggagaa acattaggta
    11521 ttgttgggaa aactggtgcg ggaaaaacga cgatcatcaa attactgatg cgtgaatacg
    11581 atgattatca agggaaaatc caatttggcg gacgttcgat caaagaatat tcattgaatg
    11641 ctttattagg atcaatcggc tatgtgcctc aggatcactt cttgttttcg atgaccattc
    11701 gagataatat ccgtttttct aatccaaaac tttcagaaga agaagtagag catgcggctt
    11761 ctttagcatt tatcaacaat gaaatcaagg aattaccaaa aggttatgat accttagttg
    11821 gagaacgtgg agtctcttta tctggcggac agaaacaaag gatttcgatt gctcgagcat
    11881 tgatcaaaaa tcctgaatta ttaatattag atgatgcact ctctgcagta gatgccaaaa
    11941 cagaagaagc tatcttgcat aatttaaaag aaataagaaa agagaaaaca actatcattg
    12001 cagctcaccg tttaagtagc gtgatgcatg caaaagaaat cattgttgtc gatcaaggga
    12061 aaatcattga acgaggaacc catcatgagc tgcttgcact aggcggctgg tatgaacaaa
    12121 tgtgggagaa acagcaatta gaagcgaaga tagaaggagg cgaggcctga tggatgaaaa
    12181 ttatcaatcg gaatggacaa aatcgattcc acttaaagaa cagaaaaaga tcatcaaacg
    12241 actgatgaag ttcgccaaac cttttagaag aacgttcatc gttgctatct tatttgcgtt
    12301 tgctttgtct gtgatcaatg tcctattgcc aaggatcatc caaactttta tggatgatca
    12361 tctggctaaa cagtcggcta cgacacaagt tattttgttt tttgctggta tgtacttatt
    12421 tggagtcata gtaaaaagta tcatttggtt tttccaatgg tatttgtatt ccatggcctc
    12481 attgaagacc tatcagtata ttagggtaaa attatttgaa aagctgcata cactaggtat
    12541 gcgttatttt gatcagacac cggcaggatc aaccgtttct cgagtgacga atgacacaga
    12601 aacgctattt gaattttggt acgtcttttt gatggtattg actgggatct ttgcagtcgt
    12661 ttcttcattc cttgccatgt ttcaaatcaa tggagaaatt gccttgtgga ctttggtgtt
    12721 tttgccgatt ttaggaatcg tcatctggta ttaccagaaa ttcagttctc gtatttaccg
    12781 aaatatgcgg gaaaaattaa gtcagctgaa cacgaaattg aatgaatcga tttccggcat
    12841 gcgtattatc cagcagtttc gccaagaagc tcgtttagct aaagaatttg aacaagtgaa
    12901 caatgagtat ttagacacgc gttatgcgat gatcaaaacg aattcgattc tgttaagtcc
    12961 gatcatcaat tttctatatg cattggcgat tgcgttggtg ttgactttgt ttgggatcga
    13021 tgcgttgcat tctcccgtag aagttggact gatctatgca tttacgacct atgtccaggg
    13081 attttttaat ccaatgtctc aaatgatgga ctttttgagc atcttcacag atgggatggt
    13141 tgctggtagt cgtatcttga agattatgga tacggaagaa tatgcaccac aacaaaaccc
    13201 tggagcagat caaatgatta cttcaggtaa gattgagttt cgcaatgtaa gcttttctta
    13261 tgatggagag cacaatgtat taaacaatat ctcttttgtt gctaatccgg gagagaccgt
    13321 tgcattagtg ggacacaccg gaagcggaaa aagctcgatc atcaacgtat tgatgcggtt
    13381 ttacgaattt tatgaaggtc agatattgat tgatgatcat gacattcgag aatatccaat
    13441 ggaagagtta cgtaagaaaa tggggctggt cttgcaagat gctttcttgt tttatgggga
    13501 tatcgcagga aatattcgga tgatgaatcc agctatcaca gacgaacaga tacgccatgc
    13561 ggctgagttt gttcaagcaa acaaattcat cgatgaatta ccaaaaggat atcactcaaa
    13621 agtgatcgaa catggtgcca gctattctag tggacaacga cagctgatca cttttgcgcg
    13681 aacaatcgtg actgatccta aaattctagt attggatgaa gcaacagcaa atatcgatac
    13741 tgaaacagaa ggattgatcc aagaaggatt ggcaaaaatg cgtcaaggac ggacaacgat
    13801 tgcgattgcc catcggctgt caacgattcg agatgcagaa ttgattttag ttttagacaa
    13861 aggtcatata gtagaacgcg ggaatcatga aacacttcta gcgcaaaatg gtttgtatgc
    13921 agatatgtac aaactgcaaa gcagtggtat ataaaagatg ttgtgacagt agaattttga
    13981 cctaagcact agagtgaaaa taaaacatag cttctcatat ttttcatctg tgagagttag
    14041 tgtcgtagtc accagattta aaaaaagatt cgacagctag tcagaagtgc ctaatagcca
    14101 ctaagaaagc gttgacaaaa gcatttgctt tcgataatat aaagaaaacg atacaacttg
    14161 gatagtgatt gcctgatgca agctaaacct gatgagtctc ccatggtttc atggacggct
    14221 ttttgggttt ttctttttgt tttggataca gccctataca tcatggaaag gagcaaaaca
    14281 tgctgattga gaaagtactg aataacaacg tcgtaataac cagggatgaa agaaatcagg
    14341 aaatgatcgt gatgggaaaa ggtttggcat tcaaaaagaa agtgggtgac ccgatttttg
    14401 actcatgcat cgacaaaaaa tttcagttga caaatgacgg gctttcacaa cagttccaag
    14461 aattggcaca agcgattcct cttgagtact tagaaatttc ttgtgaaatc attgaatata
    14521 caaaagaggc tctacaagtc cagttgaatg attctgtgta tattgcattg acggatcatt
    14581 tgtatacggc tatcgaacgt cacaaaatgg gaatatttgt accaaatgta ttgctgtatg
    14641 atatcaaaca actatttcct aaagagtatg ccatcgggaa aaaaacagtg aagaaaatct
    14701 cggataaata ccacatcaag ttatcagaag atgaagccgg atttatcgca ttgcatttag
    14761 tcaatgcaca aggcattcaa agccagcaag acaataccta cctgtttact ataacaatac
    14821 aggatattct aaatatcgtc cgctattact ataagattac atttgatgaa gattcagtcc
    14881 atttttttcg attcgtcaca cacttacgtt tttttctgac acgcgtccta gatggagaaa
    14941 aatcagaagt gggagaagtt tctgaggaat tgtttcaggt tattcaaaaa aaatataagg
    15001 atacgtttgg ttgcgttgag aaaatagcag actttttagc cgagacgttc aactaccaga
    15061 tgtccacgga cgaaaaaatg tatttaacga tccatactgc gcgtttagtg catgagaatc
    15121 gttgtaaata aaaaaaggag gataaattaa caagaacgta tgaaataagg gtagtgacat
    15181 tcagttggct aaaccttcca ctaataaatt ggagggaata gaaatgggaa aataccaaga
    15241 attagcgaga aaaatcgtag aaaatgtcgg cgggaaggaa aatatcaatg gtttgacaca
    15301 ttgtatcaca aggcttcgtt tcaaattaaa aaacgaagaa aaagcaaatg atgagatctt
    15361 gaaaaatatg gacggtattg taacggtaat gcgagcggga gggcagtatc aagttgtcat
    15421 tgggaatcat gtgccagtcg tgtttgaaga ggttataaaa gcaggaaacc ttacttttga
    15481 tgaggctgtt tcaacagaaa aaatgagacc gtttgatatg cttattgatg gaatatcggg
    15541 ctgtttccag ccattccttg ctatcctagc tgctggcggg atgattcgtg gactgacagc
    15601 attccttgtc ttcttaggcg cgtttgaccg cggttctggc acatttgtta tgtttgataa
    15661 tatcggtgat tctgtattcc aatttatgcc tgttattatc ggtttaacag ctgcccgtaa
    15721 atttaaagta aatgaattcg tagggatgtt gcttggcgca gcattaatga atcctagctt
    15781 gtcgctggaa gctttgagtg gtgcagcaga agcaccacta acgaccatat tttctggcac
    15841 tatttttgaa gcacctattt atcaaacagt attcggcatt ccctggattg cgcgtaacta
    15901 cgcttcttct gtcattccca ttatctttat tgtgttgtta gctagccaaa ttcagaaacc
    15961 aattaaaaag ttagtacctg agatgatcgc gaatttcttc gttccattct tcaccgttct
    16021 tattacaatg cctttaggtt ttttactcgt tggaccagta ttcacattcg caactgatat
    16081 tttaatggct ggatttgaaa cacttttagc actttcgcca gttatttatg gagcaatcgt
    16141 aggcttcttc tggcaaattc tggtgatgtt tggtctccat tgggcaatcg taccaatggg
    16201 attgatgcaa ttttctgtga atggttggca aaatattatg acacctgttg cagtagtgtc
    16261 ttttggacaa actgcagcac ttaccgcact ctactttaag cttagaaatc caaaagacaa
    16321 agcaattgca ataccagcca ttgtttctgg tattgtgggg attacagagc cagctattta
    16381 tgggtttaca ttaccacgta aaaaaatatt tatttttacc tgtgtcggtg gtgcgttagg
    16441 gggcgcgtat agtgggttaa tgaatttaac ttcttggaat caaggtggac taggcatctt
    16501 tacaattccc aactatatta gaccagatgg tgatttaaca gatgtgatta atgtattgat
    16561 tgggatcgcc attgcgatgg tggtatcctt tacgctgact tttttctttt ggaaggatga
    16621 agcaggtgaa acagatgata tacagaaaaa atcaggtaaa gaaattgtaa aaacaccgat
    16681 acaaggacaa atcgctccac taaatgcagc aaaggatgca gcttttgcac aaggaacact
    16741 aggtcgagga atcttgatct accctgaaaa aggagaagta cgtgcccctt ttgacggaac
    16801 agtcatgacc ctgttcccga cgaaacaggc aattggaatg gtttctgaaa cagggctgga
    16861 attgctgatt catgtcggat tggatactgt gcagttggaa gggaaatatt ttgaaagctt
    16921 agtgcagcaa ggataaagag tgaaacaagg cgatctgttg ctacgcttcg acaaaaagaa
    16981 aatcgaaaag gaaggataca gtttagaaac acctgtgatc gtcaccaatt acacggatta
    17041 tttggatatt ttggaaaacc aaggggagac ggtcgggcca gacgatgcac ttatcaccgc
    17101 actgacctga acggttaggt gaagaatgga ctaggcttaa ctattctttt tattccactt
    17161 ctttattttc aaaattgaaa ggctctttca tgatggctga gctgataggg cgtttatcga
    17221 atttttgacc gacagaaatt agatggacta aattctgttg ataattttct gcaaaaacac
    17281 aactatataa cacgtctaga agataacaaa ttgatgtatt gatcgtaaaa ttaccaattt
    17341 ttgaatacag tcgctcgcgt gtcgtaagtc gcaaagcggc tgtactcatt gcagatagcg
    17401 ttgaatcacc gatactggtc aaagctaaaa ttggaacatg attttgtttc aatatttctg
    17461 cggtacgaag gatcacattg ttttcacccg tataagaaat cagaatcccg cacatatcac
    17521 tcgtgcaatt gtacgcttca tagaatgatt caccaaaagt ggtgacaatc gtgacatcca
    17581 ttttgatgcg cttcattttc agcatgaaat cttgagaaat caaagtattc gagttactgg
    17641 cgaaaatcac gatttttttt gcttgaagca ttagttgttt ggctttttgc agttggtcat
    17701 gatgaatgag agaaagtgta tcatgaatcg tcatctgttc taaagaagcc attttatttg
    17761 cgatcgtcat aataccgtct gtggtggaaa aaggaagatt cgcatctacg tcttgaaaat
    17821 tcccttcaag ataggctgtt tcagctaaaa aagcattacg aaaactgacc cagccatcaa
    17881 aacccatttt ttttgctaca cggatgagtg tagaggggtg gacataacat cgctcagcaa
    17941 tcgcttggat ggtcatttcg ccaagtgctg gagaatcgct taagagaaac tggatcactt
    18001 cattttctga aggagaaaag gagatttctt tgattttttc acgtaataac atgtagatac
    18061 cttcttctta tttttttacg taaattataa cttttttgaa aatgatttgt aaacggattt
    18121 atactatata acttaaaata gacatttttt gacaagatat ttacaaaaat ccactagaaa
    18181 acagtaaatt ttgtaaatgt tactggaaag aaacagattt ctattgtttg ttttgtttac
    18241 aaaaaagata atgaaagtgt aaaaaagtaa aggagtgaga acatggcaaa aggaatcaaa
    18301 atcgctacga ttggtggagg cagcagttac acccccgagt tgatggaagg atttatcaaa
    18361 agatatgagg aattaccgat acgagaaatc tggctcgtag atgtagaaga aggaaaagaa
    18421 aagcttgaga tcgttagtca gatgtctcag cgtatgtggg atgcttctcc gtatgaagtc
    18481 aagatctatg caactctgga tcgggaagaa gcattgaaag atgcggattt tgttacgact
    18541 cagtttcgtg tcggtcaatt agatgcacga atcaaagacg agcggatccc tagttattat
    18601 gggatgattg ggcaagaaac aaatggtgct ggcgggatat tcaaagcttt tcgtaccgtt
    18661 ccagtcgttt tggcaatcgt tgaggacatg aagcgtttat gtccaaatgc ttggctgatc
    18721 aatttcgcga acccaagtgg gatggtgaca gaagcagtta tcagatatgg caaatgggat
    18781 aaagtgattg gattatgcaa tgtgccagtg atggcgatga tgacagaacc agcaatgctt
    18841 ggcaagaaaa aagaagagct gatctacaag tttgccggtc taaatcattt ccattggcac
    18901 aaagtagcag ataataaagg aaatgaccgc acggcagaat tgattgatcg tttatacgat
    18961 gaagagaatg gattaccgaa aaatatcttt gatatcccgt ttttcaaaga acaattaaaa
    19021 caaatgaaca tgatcccttg tggctaccac cgctattact atcgtgaaga agaaatgctg
    19081 gaacatgctt tagaagaata tcaaacgatt ggtacaagag cgcagcaagt gaaagaaaca
    19141 gaagcagagc tatttgaatt atacaaagat ccagaattag accataaacc tgaacaattg
    19201 caaaaacgag gtggcgctta ttattcagac gctgcctgtg aaacgattgc ttcaatctat
    19261 ggaaataaag aaacacagat cgtggtatct acaaagaatg atggagctgt tcctgacctg
    19321 ccggcagatt gtgtagtaga agtcactgcc tatgttggcg cacaaggagc gagaaatgta
    19381 gcgtttggtt ctcttcctgc tgctgaaaga ggctggttgc aagtaatgaa gaatatggaa
    19441 ttgctgacga tcgaagcagc tgtgactggt gattatggga cagctttaca ggcgtttacg
    19501 atcaatccac ttatacgatc aggaaagaca gcaaaacgga tcatggatga gttgtttatt
    19561 gcacaccagc agtatttacc gcaattcaag gagacaatca gccgattaaa agaagaagga
    19621 atcgaagttc aagatgaact ggcaagagaa ttatcaggta atacacatat aatggaggaa
    19681 gtataatggg attcagaaaa gattttttat ggggcggtgc tacagcagcc aatcaatgtg
    19741 agggcggtta tgatgaaggt ggacgcggac tagcaaacgt agatgttgtg ccaatcggaa
    19801 aagaccgttt tcctgttatc accggaaaga aaaaaatgtt tgaatttgat gaggatcatt
    19861 tttatccagc aaaaattgca atcgatatgt atcatcgtta taaagaagat attgctttgt
    19921 ttgcagaaat ggggttcaag acttatcgaa tgtccatcgc ctggagtcgt atttttccaa
    19981 tgggggatga gaaagagccg aatgaagctg gcttgaaatt ttatgaggat gcctttaaag
    20041 aatgccaaaa gcacggaatt gagccacttg tgacgattac gcattttgat tgtccaatgc
    20101 atttgatcaa agaatatggt gcgtggcgtt ccagagaact cgttggtttt tatgaaaatc
    20161 tgtgtcgagt catcttcaat cgctataaag gacttgtcaa atattggctg acattcaatg
    20221 agatcaatat gatccttcat gcaccattca tgggcgcagg gctgtatttt gaaaaaggag
    20281 aaaatgaaga acaaatcaaa tatcaagcgg cacatcatga gttggttgca agtgccatcg
    20341 caacgaagat tgctcatgaa gtagatccgg agaacaaagt aggctgtatg ctggcagcag
    20401 gagcatatta tccatacact tgcaatcctg aagatgtttg ggcttctaga aaatcagatc
    20461 gagaaaatta tttcttcatt gatgtccagt cacgaggaga atatcccgct tatgcattga
    20521 aagaaatgga gcgaaatggc attcagatcg aaatggaacc aggggatgcc gcattgctga
    20581 aagaacatac tgttgatttt atctcttttt cttattatgc ttcccgagtt tcgactaccg
    20641 acaacgaact cttggaacag actgcaggaa atatctttgc ttctgtcaaa aatccatatc
    20701 tgcaagcaag tgaatggggg tggcaaattg atccattagg cttgagaata acgatgaatg
    20761 atatttatga tcgttatcaa aaaccattgt ttattgtaga aaacggctta ggtgcagtcg
    20821 atacgccaga tgaaaatggt tatgtggaag atgattaccg aatcgattac ctggcagcgc
    20881 atatccaggc gatgaaggat gcagtagaac tagacggagt cgatctatta ggctatacga
    20941 cttggggatg tattgattta gtatcagctg gtaccggtga gatgaagaaa aggtacggat
    21001 tcatttatgt cgatcgtgat aaccaaggaa atggcacatt agcccgttca aagaaaaaat
    21061 cattcaactg gtataaaaaa gtaattgatt caaatggaga aaatctttcg aacgattgat
    21121 caatgattta taaaagtcta ttgtttttca gcggattttt cgagtataat aaagctattc
    21181 gagaaacgca agcagaacaa tagacttttt ttcgcattgg ctgtaaagta tgcttacttg
    21241 attaagtaaa ggagaatcta catatgtatg atgtaatcgt tattggcgca ggtcctgctg
    21301 ggatgactgc tgctttatat gcttcacgtt caaacttatc cgtattgatg atcgaacaag
    21361 gtgcccctgg cggccaaatg aacaatacgg cagaaatcga aaattatcct ggattcgatt
    21421 cgatcatggg ccccgatcta gcttataaga tgtatgaaag tgtctcaaga tttgggacag
    21481 aaaatgctta tggaatcgtt caagatatca aggatcacgg cagccataaa gaagtcatct
    21541 gtgaagataa aagttacgaa ggaaaaacag tcattatcgc aacaggatgt atccaccgta
    21601 aattaggcgt acctggcgag gaagaatttg ctggacgagg cgtttcttac tgtgcagtct
    21661 gtgatggtgc gtttttccgt aacaaacgtc tagtagtcat cgggggtggg gactcagcag
    21721 tagaagaagc catctatttg acacaatttg cttctgaagt agtgatcgtc caccgtagag
    21781 atgaattacg tgcacaaaag atcatccaag accgtgcatt tgcaaatgaa aaaatttcct
    21841 ttatgtggga tacagtcgtt gacgaaatcg ttggaaacga aatggtagtg acaggtgtga
    21901 aagcacgtaa tgtcaaaacc ggcaatgaat ttgatttagc agcagacgga gtattcatct
    21961 atgttggact ggatccatta actgcacctt ttagagaagc aggtttgacg aacgaagctg
    22021 gctggattcc aacagacaac gaaatgaaaa cgaatatgcc aggcgttttt gccatcggag
    22081 atgttcgtga aaaagatttg cgacaaatca ctacagctgt tggtgacggc ggtattgctg
    22141 gacaacaagt gtataaatac ttggagtctt tagaagaaac aacagcataa gtttaaaaag
    22201 agtagaagaa cgggttcaat tgttctttta ctcttttttt tcgcgtgaaa tttactgaat
    22261 ctgatttatg cgaacacgtc caacaccttt tgataaaaga atcctttctt gatttaagcg
    22321 ttttttggtc ctcatacttt catatctaat aagtgaatgc tatacttatt aagaaccata
    22381 tttcagaaag aggtgttatt ttcatgtctt gggaacaagt gtatgaacaa tgggtacatg
    22441 aagaaaattt agaagaaaac ttaaaaaaac aattaaatga tctgggtgaa gatcctgaaa
    22501 aacttgaaga tgctttttat gcaccgttgg aattcggtac tgcaggtatg cggggaatct
    22561 taggacctgg gatcaatcga atgaacatct atacggtaag acaagcaaca gaaggactag
    22621 cacgtttcat ggatacacaa ggtgaagaaa caaaacgccg aggtgtcgct atcgcttatg
    22681 actcacgcca tcagtcacca gaatttgcaa tggaagcagc caaaacacta gcacatcaca
    22741 atatcccgtc ttatgtattc gaaagtctgc gtccaacacc agagctttct tttgcagttc
    22801 gtcatttgaa gacgtttgcg ggaatcatgg taaccgcatc tcacaaccca gcggcttaca
    22861 atggatataa agtctacgga gaagatggcg gacaaatgcc tcctgcagac gcagatgcat
    22921 tgacgaaata tgtccgtgaa gtcagcaatc cgctaaaagt cgaagtatta tctgacgaag
    22981 aagctaaaca tagcgggttg atcacgatca tcggagaaga agtggacgct gcttatctag
    23041 aagaaatcaa agcagtgacg attgatcgtg aattggttga aacaatgggg aaagatttga
    23101 aacttgtgta cacgccactt catggaacag ggaaaatgtt gggcgaacgt gcgttgaaac
    23161 aagctggatt cgaacaattc gtactagttc ccgaacaagc tgtggcagat ccagatttca
    23221 ctacggtcaa atcgccaaat ccagaagaac attcagcttt tgaatatgct atacgacttg
    23281 gagaaaaaga aggagctgac ttgctgattg caacagatcc tgatgcagac cgtttaggcg
    23341 cagcggttcg tttgccagat ggttcttatg aagtattgac tgggaaccaa atcggagcat
    23401 tgtttgctca atatattttg gaagcacatc aatcaagagg cactttacca gaaaacgcag
    23461 cggtattgaa atcaatcgtt tccagcgaat tgcctacagc aattgcggaa cactacggtg
    23521 ctaccatgtt caatgtgttg acagggttta aattcattgc tgaaaaaatc caacaatttg
    23581 aagaagacca ttcacatact tttatgtttg gattcgaaga aagttatggt tatttagtta
    23641 aaccttttgt tcgcgataaa gacgcgatcc aagcattgct tttattagca gaagtagcag
    23701 cttactataa aaaacaaggc aaaacgttgt atgacggctt gcaaacgatt tttgaaacct
    23761 atggctattt cgcagaaaaa acgatttctg tgacaatgag cggacaagaa ggttctgcga
    23821 aaatctctgc tttgatggaa aaattccgta aagaagcacc gacagaattt gccggagcaa
    23881 aagttattca aacagaagac tttaaagaat tgacgagaac agaccgtgaa ggaaacgtag
    23941 aaaaactaac aacaccgcca tccgacgtat tgaagtatac attggaagaa aatggctgga
    24001 ttgctatccg cccttcagga actgagccga aaatcaagtt ttatattggt gtaaaaggaa
    24061 gttctacaga agacgcagcc gaaaaagtag cctcttatga agcgattatc aaaaagatga
    24121 cagaagaata aaaaacttga gactgatgga aaaagtggta aactttagaa gtcagtcttt
    24181 tttcaagttg gaggtgaatg gattgccaga cacgcaaaaa tcaaaagtag agaaagtcag
    24241 tcaattattc aaaatgttga gtgatcccac tcgtcttaaa attctgctat atctgaaaga
    24301 cggggaacaa aatgtgacag caatcaccca agcagtggaa atggagcagt ctgctgtatc
    24361 gcatcaacta cgtttattaa gagaaaatca tgtcgtcaag tctcatcgcg agggaaaagc
    24421 gattttgtat agtcttgatg attctcatgt actggatatt ttgaaccaga cactcaaaca
    24481 tgtggaacat caataacgat aggtgcaaat agataaacac tggagtgtga caacgaagaa
    24541 atttcgttgt cacactccag tgttttttaa gaaaaagaca aaaatgatca atcgcttttt
    24601 tctgttgttt cagttccata ataactatga tcttctaggt ctaaagcatt caaaataagc
    24661 gaagccgttt cttcaataga taaagatgct acattgatca ctacacatcc tagtttttca
    24721 taaagatcat tcgcaaaatc gagttcagct ctaatttttt cgatatcgga ataagcagta
    24781 tcagggttta atccgtaaga gcgcatccgt tctttacgga tattatttaa aatatctggg
    24841 ttatttgtta gtcccacgat ttttttagga tcgatctccc aaagctgttt gggaatatgt
    24901 gcttgtggga tcaaaggtaa attggcaact tttaaatttt tgttagctaa aaacaagctc
    24961 aaaggcgttt tggaagttcg ggatacgcct agcaaaacga catctgcttc taaaaaacca
    25021 cgaggatctt tcccatcatc gtattttaca gcaaattcca ttgctttgat ccgtttgaaa
    25081 tagttctcat tcaataaatg gagggcacca ggttgccgaa ttggcttgat accagttaaa
    25141 cgttcaattt ctgatatcgg tggcgtcatc acatcaaaac taaataattg atgttcttca
    25201 caaaaagaac gtgttatctc gatcagttct tgattgatta atgtatgcag gacaagtgct
    25261 ttttcttcta atgcatcttg caatgcctgc cttaatgttt tttcatctgt cacgaacgtt
    25321 ctgcggaata ggttgaactc aacggtagga tactgagcca tagtagcttg cgctagtttt
    25381 gtcgcagttt ctccagcaga gtcggagatg acaaaaaaag taacgatttc tgtagaatcg
    25441 gaagtcatgg ataaaacctc acttgcattt tttttatgtt tattatacca tactctagaa
    25501 tgcgtacttc gtaatcattt tgatttggag ggataagtca tgaaaaaaca gacattgatc
    25561 gaacaatcat tagaaacatt gaaaaataac ggttttaaat atactaaaaa aagagaagca
    25621 ttgttaactt atttagtgaa gcgtaaccgc tacgtatctg ctaaagaagt ttttgactat
    25681 atgaatcagg aattcaaagg ggtaagttat gatacgattt accggaatct ccatgatttt
    25741 gcagagatcg atttactgga agaaaccgat ttaaatggag aaatgaagtt tcgttttcat
    25801 tgctgtcagg ggaaaatcgg ccatcaccat cattttatct gtacagtgtg cggaacaacg
    25861 aaagaaatcc atatgtgtcc gatgaattat ttcgaagaac agctaggtgg atgcacgatc
    25921 gaagggcata gattcgagat tttcggaaga tgtgaaaatt gtcaataagg atttaaataa
    25981 aataacattt gataatttta aaaaaaagca agatttcctt gacggtcaaa acagcattag
    26041 atataatatt tacaggacag tcttgttttt tgagatatat ttcaataaca attacggtta
    26101 ctttttaagc tcggagggag ggaattaaca tgtcaaaaac agtcgttcgc aaaaacgaat
    26161 cacttgacga tgctcttcgt cgcttcaaac gttccgtgtc aaaagcgggt actttacaag
    26221 aatctcgtaa acgtgaattc tacgaaaaac caagtgtaag acgcaagaaa aaatctgaag
    26281 cagctagaaa acgcaaaaaa ttctagttct gtgatgaatt ggagttggta gtgtgtcact
    26341 actaagtaga ttaaacgatg acatgaaagc agcgatgaaa gcgaaagata aggaatcttt
    26401 acaagtcatc cgaatgatca agtcatctat acaaaacgag cagattaaag aaggtcacga
    26461 cttgacagaa gaagaagaac taacggtgtt gtctcgtgaa atgaaacagc gacgtgattc
    26521 tttgcatgag tttgaagaag caggacgtga cgatttagct gaaaaagtaa aaagtgaaat
    26581 tgtaatagtc gaaaaatata tgccggaaca actttccgac gaagaaattc gacaacttgt
    26641 tcaagaagca atcactcaaa ctggtgcttc atcaatgaaa gaatttggca aagttatggg
    26701 tgcaattatg cctaaagtca aaggaaaagc agatggaaac caagtgaatg cgattgtgaa
    26761 agaactatta caatcttaat tttatcattt aaactgcgga cagtgattat ccgcagtttt
    26821 ttgtgttcgt aaaaaaagaa ctaaaaaaca gctagaagaa agaatcttat tctt
//
LOCUS       DACDIH010000031        26203 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDIH010000031 DACDIH010000000
VERSION     DACDIH010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.031"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1330)
                     /locus_tag="IV281_001434"
     CDS             complement(<1..1330)
                     /locus_tag="IV281_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8780432.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNE"
     gene            1478..2646
                     /locus_tag="IV281_001435"
                     /pseudo
     CDS             1478..2646
                     /locus_tag="IV281_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(2772..3851)
                     /locus_tag="IV281_001436"
     CDS             complement(2772..3851)
                     /locus_tag="IV281_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8780433.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(3954..4394)
                     /locus_tag="IV281_001437"
     CDS             complement(3954..4394)
                     /locus_tag="IV281_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8780434.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(4451..4861)
                     /locus_tag="IV281_001438"
     CDS             complement(4451..4861)
                     /locus_tag="IV281_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8780435.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(5041..5475)
                     /locus_tag="IV281_001439"
     CDS             complement(5041..5475)
                     /locus_tag="IV281_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8780436.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(5575..6423)
                     /locus_tag="IV281_001440"
     CDS             complement(5575..6423)
                     /locus_tag="IV281_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8780437.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            6812..8578
                     /locus_tag="IV281_001441"
     CDS             6812..8578
                     /locus_tag="IV281_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP8780438.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            8680..9939
                     /locus_tag="IV281_001442"
     CDS             8680..9939
                     /locus_tag="IV281_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8780439.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            10086..11387
                     /locus_tag="IV281_001443"
     CDS             10086..11387
                     /locus_tag="IV281_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8780440.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            11390..12241
                     /locus_tag="IV281_001444"
     CDS             11390..12241
                     /locus_tag="IV281_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8780441.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            12254..13069
                     /locus_tag="IV281_001445"
     CDS             12254..13069
                     /locus_tag="IV281_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP8780442.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            13220..14386
                     /locus_tag="IV281_001446"
     CDS             13220..14386
                     /locus_tag="IV281_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8780443.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            14616..15263
                     /locus_tag="IV281_001447"
     CDS             14616..15263
                     /locus_tag="IV281_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP8780444.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            15347..16291
                     /locus_tag="IV281_001448"
     CDS             15347..16291
                     /locus_tag="IV281_001448"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP8780445.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            16426..17877
                     /locus_tag="IV281_001449"
     CDS             16426..17877
                     /locus_tag="IV281_001449"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP8780446.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            17898..18734
                     /locus_tag="IV281_001450"
     CDS             17898..18734
                     /locus_tag="IV281_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8780447.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            18734..19393
                     /locus_tag="IV281_001451"
     CDS             18734..19393
                     /locus_tag="IV281_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8780448.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            19519..20208
                     /locus_tag="IV281_001452"
     CDS             19519..20208
                     /locus_tag="IV281_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780449.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            20214..21170
                     /locus_tag="IV281_001453"
     CDS             20214..21170
                     /locus_tag="IV281_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP8780450.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            21214..21726
                     /locus_tag="IV281_001454"
     CDS             21214..21726
                     /locus_tag="IV281_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8780451.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(21982..22530)
                     /locus_tag="IV281_001455"
     CDS             complement(21982..22530)
                     /locus_tag="IV281_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8780452.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(22619..23800)
                     /locus_tag="IV281_001456"
     CDS             complement(22619..23800)
                     /locus_tag="IV281_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP8780453.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(24036..25544)
                     /locus_tag="IV281_001457"
     CDS             complement(24036..25544)
                     /locus_tag="IV281_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP8780454.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            25703..25894
                     /locus_tag="IV281_001458"
     CDS             25703..25894
                     /locus_tag="IV281_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780455.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            26097..>26203
                     /locus_tag="IV281_001459"
     CDS             26097..>26203
                     /locus_tag="IV281_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP8780456.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIK"
BASE COUNT         8773 a         4715 c         4978 g         7737 t
ORIGIN      
        1 attcatttga aaaaatttgt tctatcttaa cgatcagatc tttactgata taaaaatcac
       61 gcgtatagtt cacaccaatc acaattcgcg gctgtttttt gtagcgatca tacaaatgta
      121 ctaggtcttc aaccatccat tgcacatttt ccttttctaa aataactggt aacaagtgtt
      181 gattttgttc tctgaaatcc atataaaatt gttcaatggc ggtttggact tctttcaaca
      241 cgctatgttc ttgataaagc atgaaagtag gaaggggcgt atttggttga ttgaagttaa
      301 gaaatccatt ataaagttta tttcgtgcga atgccaatga gtaaagaatc ttttcttttt
      361 caggtataaa caaatcaaat acagtacaaa aggactccac catttctctt gaataaagaa
      421 catgaggaac gtttgctttt tttaaaagtt tgtattgctc caagcttgtt tcttccgatt
      481 cgattttcgt gatagaaagt cgagctaaaa tattcactaa gatgcgttgt tcgcgacaaa
      541 ttggcagatt aggtcgcaaa atatctacag agggatcaat aaaaaagtca atatattcat
      601 actctgtgaa ttctttttgt acgtctttca cagaagttgc aggaaaattc tctttacata
      661 tttttaatag aaaatataat ttatctatca taccgctatt tagtcggtca agtacttctt
      721 ttttaaaaaa agtattcatc ataaaagcca ctacttgcgg atcttcatca taaaattctg
      781 tagcagtact tgaaaagatg ttccaataaa tatctaatgc tgcatattga atttgtacct
      841 cttttccaga aaaagacttt ctgccattat tagaaaagga gatagacaaa ccgtacaatg
      901 ctaaaaatgc attaatcttt ttcattttcg tatacaaata agaggcacta tagtttgttt
      961 gctgacttaa actgataatc gaatttgtct gacagctgaa caaagaagtc aagatctgat
     1021 aactgactga ttcgttgcag tatcttccaa atagacgata atagcagtaa gccggatcaa
     1081 ttccttcaaa attcaacttt aaaaaaggtt tttgcttatc aatatgcagt tctgttagat
     1141 tgagacgctc aaagtcttgt tctaattctt tgatgtatag taaaaaattt gattcagaca
     1201 tatggaaatg atctgctaaa tattgtgtag taataaagaa acgatgttca gataggaaaa
     1261 gaaaaatatc taattttgta ttgctttttt tcttcaagag aaacgtttta atcgcttctt
     1321 gagtttgcat gaaagaggct cctttttact taaattgtta aaacttgaaa atattactgt
     1381 ttttttcata aatttagaca aaaaaacagt aaacatatag gtagaatctc tactatctta
     1441 tttcttgcat acaaaaaaat caaggagtga gagagttatg agatattcaa ccatcttaat
     1501 atcaacattt gcagccttaa gcttatcgct tactataaat cagacagtcc acacagagga
     1561 gatagaaaca gccaaatgga cagaaaccaa gtgggccaac gtcaatcgaa ttacctttca
     1621 ggatgaattt tcatccttca attcaggagt cgcaaaatta agaaaaggac aaacagcgac
     1681 catcaaagca aaaattgact attcaggaga cttagcacct atactacaat cagctgttac
     1741 atttgagaat gttgatcccg ctttgtcgat tggctatgac gagaacactt tgactatcca
     1801 acacaagcaa gcgatattta ccttcaccgt tacattaaat caggacttgt taaaaccagc
     1861 gctttttact gtcaaggtat cagatggact accgaacgat catcatcatt taacgccgta
     1921 tagtcagcgg cagacaattg agcaagattc agctatcgac tcaggaggag atcttgttga
     1981 agaaccaaca gataaaccag aaaatgaaaa taagccagaa gtaccgccta cagaaaatcc
     2041 agacggagaa cagaaaccgg aaatagaacc tggagaagaa cccgatacag aaactcaacc
     2101 agagccagac aatgaatcaa aacctgaaat aacccctgga gaaaaaccag atgtagaccc
     2161 agaagaaaaa ccagatgtga ctccagaacc agatacggat tctggaaacc aaacagtacc
     2221 ggaaacaaat ccagacacag ataatgaaac tgaaaatcca gaaaaaccag aggtagatcc
     2281 agaagaaaaa ccagatgtga ctccagaacc agatacggac gcaagagacc aaggaatacc
     2341 agaaaaaata aacaaaaaaa caattcaaga ggatggaaaa aaagagagta aaaaatcaaa
     2401 tctagcaatt ctaaaaataa atgaagagca gctgaataaa aaatcacgaa tatttgatag
     2461 cgcccaatca gctgaaacct taaaaagtag caaggataca acctttgctg tcctgaaacg
     2521 aaaaataaac agctaccaaa atccggagag tcacaaaaca aagttatact gtggagcgga
     2581 atcattctat taagtatagc aacaatgctt tctgcaaaaa gatttaaaca aaacagatct
     2641 ctctaacatt tgaataatgt ctgtgaacgc atttttcaaa taaaaagaaa tatccagaaa
     2701 atgaccccaa aataccagat tgaaatctga tatttggggt cgttcatact ttatctttct
     2761 tcatatttat tttaagggat atctgtttct tttggaagat ggttcgggat ctttttcact
     2821 ttcaatacat ctaaaaagaa ggtaaagatc ggaatgccga caatcaagcc ccaaacgcca
     2881 aagaaccgtt cgctaacaag taagaccaca aatgtataaa aaatcggcag ttctgttttg
     2941 ctggacatca acttcggatt cagcacataa gactcaatta gatggacaat caaaatcgta
     3001 atcaggatat aaacaacatc tcggatgcct ccttgggaat aagctatgaa agatagagga
     3061 atacaagaaa tgataacacc tgctactggg atcagactta aaatgaaaat catgatcgct
     3121 aaactcagca gctgatgaaa gccaaataca gccaaggcga ttgtagtgag caacgtattt
     3181 aaaagagcga caacaaattg agcttctaat accaaaccaa atgtattgac aaacttatcg
     3241 gcaaaatagt aaatatcttg aaagaaccaa gaaaattcac tttttaaaaa caatcttgag
     3301 aatacaattg tttttttctt ttcaatcata aaaaagaaac ttaagatgaa tgacatagca
     3361 aacgccactg ccatttttcc tatatcctgt aaatagccaa gcgcaatcga agcgccattt
     3421 tgaagctgtt caaaaaaatc gtttcgctca atataagtat ggatatattg taaaacagga
     3481 tctgcatctt ttggctgatg ctcgtaaaaa ttaataacgg actggatcat gtcaaatgtt
     3541 tgatgaataa gcatcgggat atactttgtg aatgccagat agataaggta aacgatcagt
     3601 gtataaatca tgatagctaa aacacctgta gggattcgga caaatcgctg aatgatttga
     3661 acaagatgaa tcatcaaata agtaaagata aatgtgagca aaatggttgt aatcatactt
     3721 ctaactaaga aaagaacaaa aacgacaaat aataaaacag tgagacggcg tattttttca
     3781 ttcgccaaaa aacgctgata aacggtcaaa tcgacaacct ccaaataaca aaaaataaac
     3841 acgttattca ttatatgatg gaatatgaaa ataagaaaga gcaaaaagaa aaaccgctaa
     3901 atgacgttat cttatgagtt ggttttcctc ataaaatcgt tcatctagca gtttcagccc
     3961 tttttctggt cttgatgaaa attatctagc atttttcggg tcagatgttg cagtgtatcc
     4021 aattcttcct gttcaagtgg aagataagac ttgatctttt gtggaatacc ttctgcttgt
     4081 ttttgcaacg cagcaccttg agcagtcagc gtaacattta cgattctttc atcacccgtt
     4141 tcacgctgac gattaatata atttttttct gctaactttt ttagtaaggg agtcagcgta
     4201 ccagaatcca gacacaaatc atttcctagc tctttcactg aagtcttttt cttttcccat
     4261 aaaactaaca tcacaagata ctgggtatag gtaagattca gcggcttgag gaggggagtg
     4321 tagtgtctaa tgatttcttt agaggcggca tataaaggaa aacatacttg attaactaaa
     4381 tatagatctt ccatttttcc acctccgctg ttttactata gtgtatcata gcggatttac
     4441 acaaaaaagt ctattcacta actgctttca gagtaacttc tatatttcca gctgtagctt
     4501 ttgaatacgg acacacttta tgcgctgcct ctaataattc ttgcgtttct tccagagaaa
     4561 gaccttcgat agatccttca atctctacac ctaagacaaa gctcgtttca ctttctgaat
     4621 aaagggaaac ttgtgcactg acagtagaag ccccagagat atttttttgc tgtttgatca
     4681 attctaatgc actgttaaaa catgcactga aaccagctgc aaacaattgt tcagggttgg
     4741 ttgcattttc gacgcgttta ccaggagctg caatattcaa ttcgaatgta cgatcgggat
     4801 tatgcacttc tccagagcgt ccgcctgtgt tgatcatttt cgtagaatac atttttttca
     4861 taaaattacc acctttaatt atattttata caattgaatt ttacacaata taattgtgga
     4921 aagcaaagaa atggattggc ataagataaa taagagaaag cacatgaaac agacgcgctt
     4981 tctcgaatag caaacatata caaaaaatat cagagacaaa aaataatctc atcataaaca
     5041 ttacatatat ttctcaaaaa gttgttttct ttcttcaaaa attacttcac gatggacctg
     5101 atgaagcatc cataaataac caaaaggatc tctgaacatt gagttagtca caccaaagtc
     5161 catcatttct gtcatttctt gaatctctac acagccattt cgtaatgctt tattatgtgt
     5221 gtcctcgata tcagagacga tgatattgaa ccagatcgta tttggatgtt cagcagtagg
     5281 agcagtcaaa ccaagagacg tgttttcatc cagcatatgg aatctcgtac catacaaggt
     5341 aaaaatgact tcattattgc ctctagggta atctgttgcc tcaatcagtt gtatttcatc
     5401 aaagattgtt tgatacagtt ctagtgcttt caaactatca tttaatacaa aatcgatttc
     5461 cgttccttta tgcataaaga tctcctctcc ttttcagtcg aacagttact agattctttt
     5521 attataccag ctaaagaaaa cgcattcaaa aaaatagcgg atttacttac caactcattc
     5581 gattggatga taagtaaatc cgcgtcccat tacttctgct acgctgctga caatgacaaa
     5641 ggcacttgga tcgatttctt ctatcaatgg tttcattcgg gaaaattcat gatcagtcac
     5701 aacgatcaat aaaacctctt ttgactttcg attatacccg ccttttgcct ctagcagggt
     5761 aaggccacgt ccaatctcag cacttaagcg aaggcggatg tcttccaaat aatgttcact
     5821 catgaccata acagatttct ttcgattgat acctgtttcg atataagtca tgacaattga
     5881 agtgattgca atagataaaa tagcataaag aaactcttct attccaaaaa taaacagatt
     5941 aagagaaaca acgaatgcgt ctgtaaaaaa taagccaatc gaagtattca agtggaaata
     6001 ttttttaaaa atgaggggag ggatcgtcgt accgccgcta gaagagttgt ttttatataa
     6061 aatcgcaact cctaaagcaa aaatcgcact tccaaaaatg acagacaaca accgatctga
     6121 agtaatcatt gtttctggaa ctattgcaat gattaaagga aaaaccaagc ttccgaaaag
     6181 tactttaaaa aagggttttt tacctaaaaa tagaagagct agtaccaaca taacgatatt
     6241 caacactaaa ataactatag ctcggtcaaa gcctaaagca gcttccagta atattcctag
     6301 accgctgaca cctccagcag caatatgatg aggccctaaa aacatattga tagcgactcc
     6361 taatatgaag agggcaaaaa taatcttaat gtatgtccat ataaattgtc tcgtttcttt
     6421 catttattca cttccttaat tcattaataa tactcttatc ttattataac ttacttttca
     6481 ccttaagtta actttaatta ataaaaaaat taggctagtc taaattatta tgtttcattc
     6541 ttctagaata tacttctacg ccaaaaactg ttaaaaaact tttactttag aaaattggag
     6601 aaattgatcg cttaagataa attcaggaca ctaaatagta caggaagtag actaaaactt
     6661 ttaaaagttt taagtctatc tatttggcaa gtttaaaaaa taaaaattcg gtatcaaaaa
     6721 actgttaccg ataacatttt tgaaagcgtt gtcttttgct tctctatcac ttatagtgat
     6781 gacgtcatat taaaacacaa ggaggaaatc aatggataca gctgcaattt atcatcgacc
     6841 ggaaagtgaa tatgcatttc tatatacgaa tacaagattt cgtattcggc ttcgtactag
     6901 gaaaaatgat atcaaaaaag tatatgtgct atgtggagat ccatatacca taacaactga
     6961 aaaatggtat caaaaacaac gaccaatgaa aaaatgtcta agtaccaatg ttcatgatta
     7021 ttgggaaatt gaagtgacga gcgaaacaag tcgcttacag tatgcgttcc atgttgttgg
     7081 ggaagataac acggactgtt tttacggtga tcaaggaatt tttccttatc gagaaaatgt
     7141 gattactgaa ccaaattttt attttcgaat accttatttt catcaaatcg atcgattcac
     7201 tattccagaa tgggtaaaag aaacagtatg gtatcaaatt tttcccgaaa gatttgcaaa
     7261 cggggacaaa gcaaatgacc ccgaacacac tttaccttgg ggaagtaaag atccagaccg
     7321 agaagacttt ttcgggggag atttgcaagg tgtgatggat catttggatt acttgacgga
     7381 ccttggcatc aatggcatct atttttgtcc catatttaaa gccacatcca atcataaata
     7441 cgatacgatt gattattatg aggtggatcc cgcttttggc gataaaaaac ttttaaaaaa
     7501 cttgattgat gaagcacata aaagagggat acgtatcatg ttcgatgctg tattcaatca
     7561 tatgggtgtc cattcaccac agtggcaaga cgttctcaaa aacggagaaa aatcgatcta
     7621 taaagactgg ttccacatcc gttttttccc agtagattct tatcagatga ctgacttacc
     7681 agaaactgct gaaaatatcc catatgatac ttttgctttt actccattca tgccgaaact
     7741 aaatacagca aatccagaag tgcaaaaata tttgctggat atcgcaactt attggataaa
     7801 agaattcgat attgatggat ggcgtcttga tgttgctaat gaagtcgatc atcatttttg
     7861 gaaaaaattc agagaagcag ttacagaaat caaaccagac atctatatcc ttggagagat
     7921 ttggcattcc tctcaagctt ggctgcaagg ggatgaattc catgcagtga tgaattatgc
     7981 atttacagat tcgattaaag attattttgc aaaaaagaaa atcacagctt cacaaatggt
     8041 cagtggaatg aatcatcagc aaatgcttta cagagatcag gtgaacgaag gaacattcaa
     8101 cttattagat tctcatgata ctgccaggat cttgaccctt tgccaaggta acaaagaatt
     8161 gatgaagtct gtgatggcct ttatgtttct tcaaaaaggg tctccttgta tctattacgg
     8221 gacagaaata gggatgactg gtgaagatga cccggattgc cgcaaatgta tgatatggga
     8281 gaaggaggaa caagatctag agctattcgg ttttataaaa gaacttgttt ctctaaggaa
     8341 acagttgtct aagattattt ctgaaggttc tactcagtgg ctaatagtaa atgatcgtga
     8401 agataagctc tatttcacac ggaaactaga gggacagatc atctatgttt actttaatca
     8461 gtcaaaagaa ccttgggtag tagaacagga aaatgaggtg attcttagtc aaaattgtca
     8521 gttgttagaa gacgggaaag cagagatacg acaatacgga tttttgatca tgtgttgaaa
     8581 actgttaccg gtaacaacaa gaaagcgttt gcaaatgaag atttgtgccc attataataa
     8641 gggtgtaaca gcacgttgaa tacaaaggag gaaatgaaga tgaaaaaaag aagtgtgaaa
     8701 cagttgggtt tagggttaat ctctttagga atcttaggcg gattagcggc ttgtggcaat
     8761 ggtaactctt cagcagaagg ggaagccgat gaaggaaaaa cgttaacagt ttccgttgat
     8821 tctggatata aagattatat aaacgaaata aaaaacactt tcgaaaaaga aaacgatgtg
     8881 aaaatcaagc tagtagaaaa ggacatgttc gatcagttgg aatcattagc gttagatggg
     8941 cctgctggaa agggacctga cgttatgatg gcagcatatg accggatcgg tgcgttagga
     9001 cagcaaggac atctggcgga agtaaaactg ggaaatgaag cagcttacga tgaaacagac
     9061 aaagcccaag tgacttatga tggaaaaatc tacggagaac ctgcagtaat cgagaccctt
     9121 gttttgtact ataacaaaga tttagtcgat acagcaccat caacgtttaa agatttggag
     9181 aatctatcca aagacagtcg ttttgctttt gagtcagaag caggaaaaaa tacaggattt
     9241 ttagctaaat ggactgactt ttattattcc tatggtttaa ttgcaggtta cggtggctat
     9301 gtatttggtg atgatggcac aaatccttca gatatcggtt tgaataatgc aggtgctgta
     9361 gaaggaatct cttatgcaac agattggttc aaaaatgttt ggccaaaggg aatgcaagac
     9421 ataaaaagtg caggagactt cgccagccag caattcatgt ccaataaaac tgcagcaatc
     9481 attgacggtc catggcaagc gcaaacatac aaagaaaaca atatcaatta tggtgtagcc
     9541 aaaattccta ctctaaataa tggacagcct tatcaaccgt ttggcggagg aaaaggatgg
     9601 gtagtcagca attatgctaa aaacaaagac ctctctcaaa aatggcttga ttatgtaacg
     9661 aatcaggaaa atcaagaaaa attctttgaa atggtaaatg aaatccctgc aaaccaacaa
     9721 gctagggaaa cagcaaaggc taaaaacgat gaattaacaa cagctgtgat tgaacaatat
     9781 gaaacagctc aagcgatgcc aaacatccca gaaatgggcg aagtttggac aggtgcagaa
     9841 aacttgatgt tcgatgcagc atcaggttca aaaacaccaa aagaatccgc agatgaagca
     9901 gtaaaaacaa tcagtgaagc catagaacaa aaatactgat taaaatgata accgtccatt
     9961 tttataaaaa atggaacagg tgcttcagat aaaagtggca agagcgagat ggaaaattat
    10021 cttgctcttg ttgccattat cttaaaagca gtttgcggtt tagcaagttt agagggggca
    10081 ttcagatgca gcaagataaa acaagcaata tcaacgaaaa aaaaatcacg ctttgttcac
    10141 ttattccagg gatggggcag tttgtaaatg gccaagcttt taaaggtatc gtttttttcg
    10201 ccatattcgt cctattcatt ggtcagatgc ttctgggcgg atttcatgca ttggtaggat
    10261 tggttacttt gggaagtata ccaatggaag atcattcatt atttattttg attgaaggca
    10321 ctttgcaatt catcattaca atcattttct tgatttttta cggtttgaat cttttagacg
    10381 cttatcgagt agcgaagatg cgtcaagagg gcaaaaaaat caatcggacg atgaaggaag
    10441 tcatacataa tataggtgat gaaggttttc cttatctatt gacacttccc gcttatcttc
    10501 tcatgatttt tacgatcatt ttcccagtcc tagttacatt gttcactgca tttactaatt
    10561 atgacttcaa tcatattcca ccagccagtt taattgattg ggtaggggct aaaaactttt
    10621 ttagtatctt ctttttaagc tcatatcgta atactttttt tgcagtcttt tcgtggaccg
    10681 tgatttggac tttatgtgca acaactttgc aaatcaccct tggtattctc acagcggttg
    10741 ttgctcacca gaaatttatt aaattcaaac gtattttcgg tatcattttc ttattaccat
    10801 gggcggtccc agcttttatt actatcatga gtttttcgaa tatattcaac gatagcgtag
    10861 gtgccatcaa tactcaagtg attcctttca taaaccagct tccttttgta gatatcagtg
    10921 caatctcttg gaaaacggac ccgaactgga cgaaagtagc aattatctta gtgcagggct
    10981 ggttaggatt tccctatatc tatgtgatgg ttacaggaat cttacaatcg atttcagaag
    11041 acttatatga agcagcaaaa atcgacggag caaatgcatg gcagcgcttt tcaaacatca
    11101 cgcttcctgc tgtcttccta gtggcagcgc caacatttat tacacagtat acggggaatt
    11161 tcaataattt ctccatgatc tatctgttta acaatggcgg tcccggaagc gtaggcggag
    11221 gagctggttc tactgatatt cttatttcct ggatctataa acttacgact ggaacttctc
    11281 cgcagtattc tatggcttca gcaatcacgt tgattatctc cgtcatcgtt atctgtgtgt
    11341 cattgctggt atttaaaaag acaaatgcgt tcaatatggg ggattaacaa tgaaaaaatt
    11401 tcattcgagt gctcgattcc aaaaacgact ttctcacttt tttacttact tttttctttt
    11461 aatactgtca gtcattatca tctatccgct tttgatcaca gcaagtactg ccttcaagtc
    11521 aggaaatatt gcagctttta gtttagatct ttcaagtgac tggactttag gaaactttac
    11581 tcggctattc agtgaaacac tttacgggac ttggtataaa aatacattga tcatttctgt
    11641 cctaacaatg atcgtacaag tgactgtagt aactttggcg ggctatactt atagtcgcta
    11701 tcgttttatc ggccgcaaat caagtttgat gttcttttta gtgattcaga tggtaccgac
    11761 tatggcagca ttgactgcct tttatgtcat ggctttctta ttagaagcgc tcgataagta
    11821 ctggttcttg acaatgatct atataggcgg tggaattcca atgaatactt ggctgatgaa
    11881 aggatacttt gacactgtac caatagattt agatgaatct gctaaacttg atggggcagg
    11941 gcatttcagg atttttttcc agattgtatt accgcttgtc aaaccgatgc tcgccgttca
    12001 agcattatgg gcttttatgg gaccttttgg ggatttcatg ttagctaagt tcctgcttcg
    12061 aacacctgaa aatcagactg ttgctgtcgg cttacaaaca tttatttctg atgtgacaaa
    12121 ccaaagagtc tctctttttg ctgctggggc aattttgatt gcactaccga tttgtctatt
    12181 attcttcttt ttacaaaaga actttgttac cggcctgaca tctggcggaa caaaaggata
    12241 ggagcagagt atcatgcaaa catttccgtt aaactatttt tccgcattac gtacgccaac
    12301 tcaagtgttt gcgggacgca aactattatc atggcctaaa tttttcttga tatttgtatt
    12361 tttagtttcc ttgatggtca tgcctgtgac tcttttttat gccaatcaga tccaagcgat
    12421 tccattagaa caatttctat ccgttcattc gctgatcgat gaacaaggaa cccaaaaatt
    12481 ttctgaactt gaattatctg aaactgggtt acaagcatcg caacaaacga tagctgtgac
    12541 ccctgaaatt ttggtaggtg tgtctttatc tgagaaacaa caatccgatc atggaacttt
    12601 tattgatttt gagaaggaac aatgggtgat ccaacaaaag gataaatctg gtatacgccg
    12661 ctatacgatg aattatagcc catctttcca accagattct gtacgtacgc ccgaagattt
    12721 ccaacgattt ttagaaagag aattttatgc cagtaatcgt ccaacaatca ttttatctta
    12781 tagtttaagt cttggtcttg tcctatttgt catgacgagt ctcattttat tcggggcttc
    12841 ctttttcttg tggatgacaa gaaaaagcca actttcatcc attcatacat tcaaagagag
    12901 tgcaaatttg atgctgaatg ttatgggaat aggttccatg gcagcagcag ttgtagggtt
    12961 tatccacttt gactttatct tgatgctaag tgtccaaaca acgataactg tcttgctgct
    13021 tttatgggtt tttgtcaaaa caagattcaa agataagcgt gtcgaatgac aagtttaagc
    13081 gatttatgcg acaatgaatg tgaaaaaaat gaaaaataaa gtagtttttg aaaaatttct
    13141 gaaaaaatta aacttcttag ttgattcttt tcttttttag tattaacgta atagggtagt
    13201 ttttttaagg aggaaaatca tgttagagaa aactttatac aatcaattat tttcacgttc
    13261 tttctcgctc cctgtcgaag taaattattg ggacggtaca acgaaacaat acggagatac
    13321 ggacactcct ccccaaatca aaatcaatat ccatgaagaa atcccaatga aagagattac
    13381 aagtaacgct tctttggcac taggagaagc ctatatggat catcgaatcg agatcgaggg
    13441 aagtatccaa gcattgatca acgatgctta tcagcaagca aacagctttt tacgaggaaa
    13501 agattatcta aaatggctgc caaagaaaga aaaacatacg aaacaaaaaa acaaagaaga
    13561 tatccatgcg cattatgact taggtaacga cttttataaa ctttggctag atccaacttt
    13621 gacttattct tgtgcttatt tcaatacacc tgaagatacg ttggaacaag cacaaatcaa
    13681 taaagtccat catatcttag acaaactctt tatcaaagaa ggcgaaacgt tgcttgatat
    13741 cggttgtggc tggggaacat tgatgttcac tgctaccaaa gagtataatg tcaaggcaac
    13801 tgggatcaca ctaagtgaag aacaatacga tttgattacc aaaagaataa aagaagaaca
    13861 tttagaagat aaatgccgtg tattattaat ggattaccgc gaactaaaag gcgaaacgtt
    13921 tgatcatatc acaagtgtag gtatgttcga acatgtaggt tctgaaaatc ttgaaggtta
    13981 tttcaaagtg gtaaaagatt tattgaaacc aaaaggaaca gctttgatcc atggaatcag
    14041 ccgacaacaa ggcggtgcga caaatgcttg gatcaataaa tatattttcc ctggcggtta
    14101 tgtccctggt gttactgaat tagttgataa tattacaaaa aacgatttgc aactgattga
    14161 tttggaaagc ttacgtcgtg actaccaatt gacattagaa cattggacaa agaattttca
    14221 tcaagtccaa gatcaagtca cgaaagaaat gggtgattcc ttctatcgaa tgtgggatct
    14281 atatttacaa gcttgtgcag cttcatttga atcaagtaat atcgatgtga ttcaatattt
    14341 gcttgttcaa cctggaaaca atgacatccc aatgcatcgt gcataattga ggcaaaagta
    14401 ctgtttaaag actatttaaa aagaagctaa gttttatgat gctccagaag tgaacttttt
    14461 tgtctaactt ctaggattgt ctctaaagct tagtttcttt ttctttatat tttcataata
    14521 aaagtttggt tctttaattt ttaaacatga cagaaaattt tttcttcgtt atacttgtaa
    14581 tagaatacta ttttataaca aaggggatcg aatcaatgaa aaaaactgtt atttatacta
    14641 gtttatttgc agctttgttt ttagctgctt gtcaatcaaa taccaatcaa ggcacatctg
    14701 ggaatacgtc ttccagtagc gaacagcgct cttcacaggt atcggactcc tcaacaaaag
    14761 caagcacatc ttcgtctagc cagacaaaaa gttctagtga agcaagtact cagtcagata
    14821 tcaaagtgtc tgcagctcgt gcgatcgaat tgttccaaga taaataccca gatactgcaa
    14881 ttactagttt ggaattagat agtgattggg gatcttactt ctacaagata gaaggtgtcg
    14941 atgatcaaaa agaatacgaa gcaagaattg atgctcaaaa tgaaaaaatg gagacagaga
    15001 atccagaaac tctagatcga gatgaacaaa atggtcaaaa gaaaaaagaa gatggtctgg
    15061 atgttgataa attgatcacg attgaggaag cagcaaaaat cgccgtcgac aaagtaggaa
    15121 gcggtacggc aacagattgg gatttggata aagatttagg tactacttat tgggaagtca
    15181 aagtaaaaga cggcaatcaa agtacatcag tcaagatcaa tagccaaaca ggcgaagtac
    15241 tgtcaactga acaagatgat taagaatttc ttcggaaaac aagagttgat ttttctttta
    15301 gtcatgatac actagacaaa ataacttgtt tgaaggagcg atttacttga gtttagaaac
    15361 gacctttgcc aatcatatct ttgccaatcc tttgatgaat gcatctggtg tccactgcat
    15421 gactacccag gaattagatg aactggctca ttcagaagca ggagcattta tcactaagag
    15481 ctgtacgatc aatgaacgaa aaggaaatcc cgaaccacgc tattttgatg ttcctttagg
    15541 gagtatcaat tctatgggac ttccaaactt aggtttttct tattatctag agtatgctct
    15601 tgcgtatgaa aaagtgcagg aaaaccaaaa tcaaccgtta tttttttcca ttgccgggat
    15661 gagtgtccag gaaaacttgg aaatgcttga gaaaatagaa aaaagtgggt ttaatgggat
    15721 cactgaatta aatttatctt gtcccaatgt gcctggaaaa ccacaactag cttacgattt
    15781 tgaggccact tatgaaacac tgaaagaagt attcagcatc ttttctaagc cgctaggaat
    15841 caaattgcct ccttattttg attttgctca ttttgatcag atggctgata tcttgaatca
    15901 gtttccattg acctatgtca atgctatcaa cagtgtgggc aatggtttgt atatcgatac
    15961 agaacaagaa gcagtagtca tcaaaccaaa agaagggttc ggcggcattg gcggagagta
    16021 tatcaagccg actgctttgg cgaatgttcg cgctttttat acacgtttga aaccagaaat
    16081 ccaaatcatt ggtacaggtg ggattcggac aggacaagat gcatttgaac atctgctatg
    16141 tggcgcaagc atgttacaga ttggtaccga gcttcataaa gaagggccgg aaattttttc
    16201 tagaatcata aaagaattaa cgcagattat gtcagaaaaa ggatatacgt caattgacga
    16261 atttaaaggc aaattacgca ccatttcata gcaaagagac gaaggtcatg attgatcttc
    16321 gtcttttttt ataaaaaaca ctcgtctgtt cattcctgtg caagcgtact cattgtatgt
    16381 tagacgaact tgtgggaaaa taacagaggc aaaaaggagg ataatatgaa tgatgcaaca
    16441 gaattagcaa aagagcttaa agaaaaagaa tttagttttc aagaactagt tacggatata
    16501 cagaaaaaaa taaagaagca aaatcatgaa ttgaatgcgt ttgtcgcgct tgaaccattc
    16561 gagagtaatt cccacgagct attgattccc cagtcagaaa aagggccatt ggctggtatc
    16621 ccatttcctt taaaaatgct cggtcaagaa aaaaagggat ggctagctac ttcaggatct
    16681 cgtttatttg aaacacaccg agcttctcat acatctaatt atgtgcggca agcagaagcg
    16741 ataggtctag ttccttttgg acaaaccaat gcgccagagt ttggtttcaa aaatattaca
    16801 gacccagtca tctatggccc agcaagaaac ccttggaacc ttgaatgtac accgggcgga
    16861 tcaagtggag gagctgctgc tgcagtttca tcaggaatcg ttcctttagc tggtgctagt
    16921 gatggcggtg gttctattag aatacccgct tcgttttgcg gattgattgg gctgaaacct
    16981 tcgcgaggga cgatgccagt tggtccatat gcttggagag gatggcaagg agctgctatt
    17041 gatttcggct tgacagtttc tatgagagac acggaggctt tattttatgg gatgagaagt
    17101 atccattccg gtgcgccata tcaagtatcg ccagttgagt ggcaaacaca tccaagaaaa
    17161 aaacggttga agatagcttt ttgtgttgat tcaccgatcc actcacagat tagtgaagag
    17221 gcagtacttg ctgtaaaaaa agctgtagat ttccttggaa aagaagggca tgatttgaca
    17281 gagattcctt acccactaga tggtcgtaaa ctgatcacgt cttattatca gatgaatggc
    17341 gcagaaacag cagcaatgat ccgctcgatt gaaacaggca tccagcgagc tgtcagaaaa
    17401 gaagaacttg aacccatttc ttggactttg ttccaatatg gtcagaagct ctctgccgct
    17461 acttacatcg attctctgca tgtatgggat gaagcagcaa ttgtcatgga aaacctacat
    17521 caagaatacg atctattcct ttcaccaacg actgcattta ccgcccctaa aatcagcgaa
    17581 gatttgcaaa gtgatgcgat aagagcacag atggaacaca tcagtgattg ttctgaagta
    17641 gaaggtttag acgttgtttc ttccatgttt gaggagagct tgaagatcac accttataca
    17701 cagttggcta atctgactgg acaaccagca atcagcttgc ctactcattt gacaaaagaa
    17761 aacatgcctc taggaatcca atttatggct gccagaggaa gagaagattt gctatttcaa
    17821 atcggctatc tatttgaagc aaatcatcag ttccatttac caaatccatg gaaatagcag
    17881 aaacaaggag aagaagaatg aagaaaaaaa gattgtttgc tctaataact agtgcctttt
    17941 ttattttaag cgcttgcggt acagctgccg ctaatcagga agataacagc tggagtaatg
    18001 tagaaaaaaa gaaagaatta gtagtagcga cttctggcac actgtttccc gcatcttatt
    18061 ataatgatca aaatgaatta gttggttatg atgtagattt ggcaaaagag gtggctaaac
    18121 ggttaaatgt caaaatctcc tttaaagaat acaacgtgga tggacaaatc acttctgttt
    18181 caaaaagaga atcagatttt gctgcgaacg attttggtat cacaaaagaa agagaaaaaa
    18241 aattctccct ttcagagact ttcaaatatt catttgacag tatgatcgta cgaaaaaaag
    18301 atgattcagg aatccactca cttgaagatc ttaaagggaa aaaagcagct ggcgaaccca
    18361 atactagcta tatgcgttta gcggaaaaac acggagctaa attagtcaca tatgacaatg
    18421 ccacgaatga tcagtattta agcgatgtag caaatggtcg aacagatgtt attctaaatg
    18481 attattatct gcagaaaatg gccgtatctg ctttaccaga tatccctgta aaaatactgg
    18541 agaacgttta tttcaatgaa aacgaaagtg gttttttatt gaaagaagga aatgatgctc
    18601 tgaaaaagaa aatcgatggc gtattaaaag agatgaaaga agatgggacg acaaagaaaa
    18661 tatctgaaaa atattttcaa actgatgttt cagtcaaacc gaaagaaacg atggctgagt
    18721 cggtgagtga gtgatgtact ttcctctaat tgatttttcc ctcatgtgga cttcacttcc
    18781 tttcgtactg caaggcttgg tatacacatt agggattggc ttcgtgtcgt ttcttttagg
    18841 aaatctggta gggttgcttt tgacagtttt aggtcttttg gattggctgc ctctgaatgt
    18901 atttattcgt ttttatttat cgttctttag aggcattccc gcactagtcc tgctgtttct
    18961 gttatatttt gggttacctt atcaattaag tgccttaacg gcttcagtca tctgctttac
    19021 gattaccagc agtgcattta ttggtgaaat ctatcgagga tcactagcag gcgtttcttc
    19081 aggtcagtgg gacgctggtt tagcttctgg tttttctttc tttggggtat tgaaatatat
    19141 cgttttgccg caagcttttc gaatctcagt accggcactc agtaacgtag ctatggatct
    19201 agtgaaaggt acttcgttag cagcaacaat cacagtgcca gaaatatttc aaaaagcaaa
    19261 gatcataggc ggtcggacat tcgattattt ttctctctat attttagttg cactgattta
    19321 ttgggtcatt tgtatcggga tcgaaaaagt gcaacagtat ttagaaaaac gttctatgca
    19381 tcagtcaatc tgaaaaatag aaatggtgct agcgtggctg gcaccatttc tattttttgt
    19441 ttcttgtata ttctgcctag tattttaaaa ccatttcatg taaaatgtaa atcgaaaata
    19501 tgttcggagg tagaaaacat gaagaccccg ctaaccatcg aaatagaatc ctcgttatac
    19561 caatattgca tcgagcaagg cggcttggtt gtagaagaag tcacgatgcc tgatgaccaa
    19621 ggaattgtag atacgcttag ctgcttgaca aaatttgatg gaagccgaga atggcgatgc
    19681 tatgagctga aagtgacaaa agcagatttt cgctctacag ccaaactgtc atttgttgga
    19741 cattataatt attttgtttt accaagtaaa ctatatgaag aagtaaaaac tgaaatccct
    19801 acaggaatag gtgttttagt gtatcgtccg tacacggttg aagaggagat gcctgcctct
    19861 ggtacatttc tgattgcgaa aaaagcagca aagaaagagc ttgctgtacc ggaagaagct
    19921 ttgacaaacc gcttcatggc ttctttattc agggaagtaa gaaaagcaaa gcaaatggag
    19981 cgaggcgtaa aatattttcc aacagatcaa ttgtacaaag aattgaaaag acgttcagaa
    20041 aatcaagatc cattttctaa cgaacgatat tatgaacggt ttttagagga aatagctagt
    20101 caggcaatca atgagttgca agaagaagtt atcgcgcttc agcatgatta tgaatatttg
    20161 aggcaacaac agcagatccg tcggttgcct acagagccat tggagtgaaa aatatgtact
    20221 atccttattt aagaggaaaa caatttgacc tattagcatt aaaagaagca ctgtcgcgag
    20281 gattgctctc caataaaatc caaccagtga tcgaaccagt acgtgattcc gctacgctta
    20341 aaaatgtgat cgaattattt caaaaaaagc accatccaat cgctgtgatc caaaatcccc
    20401 aagtaggaca attcaaatta ttcgatcagc atgtgcacag ttggacagtc aaagaaaata
    20461 gctctgtcgt ccctgcacag atcttaacgc cagaaaatct cagtgaagta ctggattcac
    20521 ctccagcgtt tcttgttttc gatggacagc actacccaaa agatacagag gtgtggaaac
    20581 agttggcagg agttgattcg aaatttttga taccagatac tagtcgcttt cgcatctggc
    20641 tgcccgaaaa caaaattgtg atccgtgact cttttcaaac gagaaagcat gttgaaagtt
    20701 atgcagataa aacagatgac ttttttagtg atgactatct ttttttccac gcagacggat
    20761 atattggatt ttctgatttc acgatcgaag gtagccgtta tttcgataaa gggttcccta
    20821 gccgagcgct tgctatccat ctaacgtata ttgatgctta cggcaatata cgcgtgaaac
    20881 attttgtctc tgacagtaac gactctgcta aagaccaagc attgaagttt cttgaagcag
    20941 gagataaaat gaaaaaatgg atcatgcgtc accatcatca actcttgatt acttcaggaa
    21001 tgattgaatt attggctctt taccagcaac aaaaattccc tggactggga gtgttgaaaa
    21061 agtggtcact tatgcatcat ttggaactta ttagtcacct gctagatcat ccaaaagatt
    21121 ggcttaaata ttattcaaaa gttcaggaac tatatgaagt gatccaatga ttagataatg
    21181 taaatgaaaa aggaatagaa aagaggaaac tggatgtata ttcggcaaac aaggaaagaa
    21241 gaagtagaag aagtggtaga aatcattaat gccgcagcgg atttcattgc ttcgcaaggc
    21301 tctcctcaat ggcaaaacgg gcagaaacca acaaaagaaa cgattttgaa cgatattgaa
    21361 agaaaagaaa gctatgtatt aatagtggaa gatcaaattg ctggaacagc tgcattagtc
    21421 tcaagcgtgg atcccgttta cacaaaaata gatggtagtt ggaaagaatc aactggtcct
    21481 tatttatcga ttcatcgtgt cgctttagca gaaagtactc gaggaaaaaa actagcaaaa
    21541 ccgttcttag aacatttgtt gacgattgca gccagcaaaa atatccaaga tatccgtatc
    21601 gatacttatc cagctaatga accgatggaa aagaccatct attctgctgg attcacttat
    21661 caagggatga ttgaatttcc ttttgaacac ggcgaaagaa aagcatatca aataatcatc
    21721 aattgactat aaaatcatct gagcaaagat aaagacatta acactactga aaaaaacaat
    21781 catatgatag ccaagaggtt gtaacagaag tggccagctt caaaaaacaa gaaagaattt
    21841 ccgaaaatgc ttctcaaatt tttgtggaat tttagcgtat tttacgaaat agctgctttc
    21901 tgaaactccc ttcatcgaga ggtcgtgaca tagcttttgt cacgacctct cggcttatcc
    21961 tacgagtatt attcgataca tttatatcaa taatcctttt tttattttat ctgcagcttc
    22021 ttggaacgct ccatcgtttg acacgactaa aagtgagggg atagctttca aacgtttcaa
    22081 ctgtgctaaa tcttctacat aaaccgccag tctggctgaa atctgttcag atgaatctcc
    22141 acgttttttc aggcgcgatt gcaatgtttc tttagagatc gtaataaaga taggacggac
    22201 taccgtcccg aaatactcat ataaatgcca aaaaccattt gctgtcaaaa cggcgtaaca
    22261 atttcctact gtcagtttct ttttaatttc tgctttactg gaaccatatt tatgttgagc
    22321 atattggtcc cactctgcaa actcatttcc cttgatcatc tgattgaatg tattgtcaga
    22381 aacaaaataa taatcctctt gatctttttc cccaggtctt ggtgatcgat tcgtgtaagt
    22441 aatgatcttt tgttccggct gaaaaatagc ttctgcgagt gaagtttttc cagaaccaga
    22501 aggaccgata aatacatagc aataattcat tgtcatttct cctttgatga ttgaacaagt
    22561 gaaaaagctt tggaaaatat ttccaaagct tttaaaagta actttgcttt tttgtatatt
    22621 ataccagcaa gccagtcaca atagcactta aaaagctgac taaagtcgct gttagcaaaa
    22681 ttttcaatcc gtgttttgcg acaagatttc ctttttcttc atttaatccc ttcatcgcac
    22741 cagaaatgat cccgatagaa gagaagttgg caaatgaaac gaggaagaca gatataattg
    22801 ctgttgtacg ctcagtaaaa tggaaattac catttgctaa ttctgtcata gcaacaaatt
    22861 catttgtaac cagtttagtc gccatgatac taccggcatc cactgcttct ttccatggga
    22921 ttccgctgat gaaggcaaga ggggcaaaga tgtaacctag gatctcttgg aaagtgatgc
    22981 cgaatatccc gtggaagata gcattgatca tggcaatcaa agcaacaaaa ccgataagca
    23041 tagcagcaac cgtgatcgcc acatggaaac catcaagaat gtattctccc aacatttgga
    23101 aaaatgtctg ttctttgttt tcttcaatga tcagctcatc ttctttttct tctaatacat
    23161 agggatttac aattgaagcc agaatataac cgccaaataa gttcaaaaca agggcagtga
    23221 tcacatattt agaatcgatc agtgccatat aagatccgac gatcgacata gaaactgtag
    23281 acatagccga tacactcatg gtgaacaaac gtttctcaga taggaagggt aattcctttt
    23341 ttatggaaat aaaaacttcc gattgtccta aaatagcaga agctactcca ttataagatt
    23401 ccaatttgcc catcccgttg attttactta atcctagacc aacagctttc ataaataatg
    23461 gaagaatctt gatatagcgt agaataccga taatagcaga aataaataca attggcatca
    23521 atacggaaag aaagaaaggt gcacttcctt tatttgctac accggcaaaa acaaagtcaa
    23581 caccttgccc agcaaaagca agaagatgat caaacatttt tgccaataca gaaacaaccg
    23641 ctgtaccaaa agttgtacgt aataatacga aaccaagaac aagctgtaaa acgaacatca
    23701 caattagtgg tttcaatttg attgcttttt tgttactgct gatcaaaaaa gcaagcccta
    23761 atgtgacaaa taaaccaata atacctaata aataactcaa ataatcactc ctattccaaa
    23821 ttccgtttta ttttacctgt tttgcaaata aaatactaat ctttagtgaa attttcatta
    23881 cttttttaga gtgaaacgca ttcattttcc attagaaaat aaagcgaaca aaataaaagg
    23941 tttaaaaagt gtaacgtctt agtaaagtct ataaaaaaac gaaactatcg attaagctaa
    24001 tagtttcgtt ttcaacattt ataagtaaac tttttctatt ggtaatatag attttcggaa
    24061 gggtagacag gtgcgcaggt aatatcgatt cctagttttt tcaatgtttg ttcatcattt
    24121 ttattgacca tcacagtaga gtgggcttgt gctccctcaa gttcagctag tttatcataa
    24181 gcaacttgag cgattggatt cgttactgca ctgattgata aagcgatcaa gacttcattg
    24241 atgcttagtg catccaaaga ggaatgaagt gtctttgatt tcaatccttg tattgtttca
    24301 agaactaatg gtgacaataa aggaatgtca tctgcgatat gggctaaatg ttttactgca
    24361 tttaagataa ctgccgctga cgcatccatc aatgaacttg tacgcccggt aatgatgact
    24421 tcatctggta attcgattgc aatcacagca gaaggatctt ttgtaggact ttgcgcttga
    24481 atcttatctg catagtcatg ggcagggact accacgtttc ggtcttcagg acgtaaaccg
    24541 acttcttcca taatcagttt gattcgatta actgcttctt cgtcgatcaa tccttttttg
    24601 aagtcgcatt ctgtttggaa ataacgacgg atgatttctt gtttagaggc ttcttggaca
    24661 acggtatcat ccacgatccc aaatcctaca cgattcacac ccatgtcagt aggagattga
    24721 taaacagatt ctttaccagt aattttttca ataatacgtt tgattacagg aaatgtctca
    24781 acatcccgat tatagttcac agctacttta ttgtaggcat caaagtggaa ggaatcgatc
    24841 atattgacat cttttagatc gactgtggct gcttcataag caatgttcag cggatgtttc
    24901 aacggaacat tccatactgg gaaagtttcg aacttggagt aaccagctgc atgtcctttt
    24961 tggctttcat gatacagttg gttcaaggaa gtagcgagct ttccacttcc tgggccagga
    25021 gctgtcacta caacgattgg tttagttgtt tcaatatact cattttttcc aaaaccatct
    25081 tctgagacga ttttttctac gttgactgga tagtcttcga tttctgagtg tttatagacc
    25141 ttgatgtttc gtctttctag tttattgata aagactttcg tggcaggctg tccactatag
    25201 cgagtgataa cgacactgtt gattaataag ccataaccac gtaattcatc gattaaccgc
    25261 aagatatcca tatcataagt aatcccataa tcaccacgga tcttattacg ttcaatatct
    25321 ccagcataga cgcaaatcag tatttcagct tgatctttca atttttgcag caatttgatt
    25381 ttcgcatctt cttcaaatcc tggaagtaca cgtttcgcat gcttgtcgtc aactagcttc
    25441 ccaccaaatt ctaaatataa tttgtcatag tggttcactc gttccaaaat atacgctgac
    25501 tgctcttcaa tatatttttc tgaatcaaaa ccaatttttt tcactaggtt ccctccttaa
    25561 gtactaaagc attttttcat ttttttgagg attcgtcaac tggaagagcg aaattcctca
    25621 aaaaaacaaa gaaaatggtt taaaccaact tgtctagata gttattcatt ggtatgatag
    25681 tttttgataa ggaaggtgct acatgaaaaa aagacagaaa aagaaaaacg catataaaca
    25741 ctatatccgt tcgattttta ccggttatga aagaatgctt gaagatcccg aattggaaca
    25801 attgacgttt acttatctaa acgaagaaac gcagctaact cgtgatgagc atcagcgcat
    25861 tcatttcaca acaagagatc tgcctagtaa ataaaaaata agataaaggt catcggattc
    25921 gattctgtcc tatcagatgg aaagactagt tgatcgaaaa aaaggctgta ccaaattttg
    25981 gtgcagcctg ttttatggag cattgcccgc tcttttttat aacaattttt tacagaaaat
    26041 ggtactattg aagtattaat aagtgaaaaa cagatgagta agaggaggca agtagtatga
    26101 aagagccaga tacaaatgca aattatgagc cagacgaact agaggagacg aatgcggatc
    26161 aggatacagg acaagaatcg tcttcttttt acgatatcaa aga
//
LOCUS       DACDIH010000032        26122 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDIH010000032 DACDIH010000000
VERSION     DACDIH010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.032"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            54..812
                     /locus_tag="IV281_001460"
     CDS             54..812
                     /locus_tag="IV281_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780457.1"
                     /translation="MSFADSSDSSTTQTIDNSITAPSETDSSENTTEETADSNISFTE
                     STNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIISSNQINNQGSQVAQQEGINGAKF
                     IVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKKLSYDQLKKVAEGETKTIDDQEG
                     VIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLLTPVSNEKGEFLTDVWLYPKSEK
                     SEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            1101..3212
                     /locus_tag="IV281_001461"
     CDS             1101..3212
                     /locus_tag="IV281_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8780458.1"
                     /translation="MEGIMENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQD
                     PVEDRLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(3250..5307)
                     /locus_tag="IV281_001462"
     CDS             complement(3250..5307)
                     /locus_tag="IV281_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP8780459.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(5421..5633)
                     /locus_tag="IV281_001463"
     CDS             complement(5421..5633)
                     /locus_tag="IV281_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780460.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            5820..6287
                     /locus_tag="IV281_001464"
     CDS             5820..6287
                     /locus_tag="IV281_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780461.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            6284..6526
                     /locus_tag="IV281_001465"
     CDS             6284..6526
                     /locus_tag="IV281_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780462.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            6544..6846
                     /locus_tag="IV281_001466"
     CDS             6544..6846
                     /locus_tag="IV281_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780463.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            6918..8972
                     /locus_tag="IV281_001467"
     CDS             6918..8972
                     /locus_tag="IV281_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780464.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            8972..11584
                     /locus_tag="IV281_001468"
     CDS             8972..11584
                     /locus_tag="IV281_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP8780465.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            11581..14112
                     /locus_tag="IV281_001469"
     CDS             11581..14112
                     /locus_tag="IV281_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780466.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            14164..14496
                     /locus_tag="IV281_001470"
     CDS             14164..14496
                     /locus_tag="IV281_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780467.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            14497..15090
                     /locus_tag="IV281_001471"
     CDS             14497..15090
                     /locus_tag="IV281_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780468.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            15253..16947
                     /locus_tag="IV281_001472"
     CDS             15253..16947
                     /locus_tag="IV281_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP8780469.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            17635..19512
                     /gene="ltrA"
                     /locus_tag="IV281_001473"
     CDS             17635..19512
                     /gene="ltrA"
                     /locus_tag="IV281_001473"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8780470.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            19643..20020
                     /locus_tag="IV281_001474"
                     /pseudo
     CDS             19643..20020
                     /locus_tag="IV281_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            20020..21225
                     /locus_tag="IV281_001475"
     CDS             20020..21225
                     /locus_tag="IV281_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8780471.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            21241..21885
                     /locus_tag="IV281_001476"
     CDS             21241..21885
                     /locus_tag="IV281_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780472.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            21896..22702
                     /locus_tag="IV281_001477"
     CDS             21896..22702
                     /locus_tag="IV281_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780473.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            22724..22954
                     /locus_tag="IV281_001478"
     CDS             22724..22954
                     /locus_tag="IV281_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780474.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            23467..23763
                     /locus_tag="IV281_001479"
     CDS             23467..23763
                     /locus_tag="IV281_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780475.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            23765..24187
                     /locus_tag="IV281_001480"
     CDS             23765..24187
                     /locus_tag="IV281_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780476.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDETISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            24206..25798
                     /locus_tag="IV281_001481"
     CDS             24206..25798
                     /locus_tag="IV281_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780477.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            25819..>26122
                     /locus_tag="IV281_001482"
     CDS             25819..>26122
                     /locus_tag="IV281_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780478.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVNGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKS"
BASE COUNT         9552 a         4097 c         5275 g         7198 t
ORIGIN      
        1 aatggctgtt tgtttgtgta gcagctctct tttctactgt agcttattcg caactgtctt
       61 tcgctgacag cagtgatagt agtaccactc aaacaataga taattcaatt actgcaccaa
      121 gcgaaacgga cagttcggag aatactacag aagaaacggc agattcaaat ataagcttta
      181 ctgaatcaac gaatccaacg tcagaaagtt cttcagagac ttcagaaaag ccgcaagacg
      241 tagaagatac gcaagtgatc aagcaagcca ttcatattca aaaaattatt tcttcgaatc
      301 aaatcaacaa tcaaggcagt caagtagccc agcaagaagg tatcaatggc gcgaagttta
      361 ttgtctacga tatcacgaat atcttagaag aaatgtcaca agatgataat gagaaaaatc
      421 aatctgtaga aataattgag tcaaaattga aagatcgagc aaaaaaactt tcatacgacc
      481 agttgaaaaa agtggctgaa ggagaaacca aaacgattga tgatcaggaa ggcgtgattg
      541 aattttccat tgaagtacct gcaaacaaaa aacaagcata ctatatcgtt aatgaatcaa
      601 gccctgaaaa catttcaaac agtgaagata ttgttttatt gacacctgtt tcaaatgaaa
      661 aaggtgaatt tttgacggat gtttggttat accctaaatc agagaaatca gagccgaaag
      721 aagaaattaa aaaagttgtg tcaactggtg taaagaagaa cttttttgaa aattgttggg
      781 aattttttgc tcaattattt tttgataatt aggatgaaat gatgaaaata ataagaatat
      841 tagtttttaa tctatccttt ttagccgcaa caatattgtg ttgcggttta attttttctc
      901 aattatcaat ttatccaaga tatcaacgat tgttcattca ttttctaaac attcaatgtg
      961 cttccatgcc attagaggtg attaaaaagc tcttgatcaa tccaattttt atggttagtt
     1021 tggttcttct tacaggattg gtaacgtggg tattaggaaa aaaagaatga aaagcttacg
     1081 atcgtttaaa aaaatattag atggagggca ttatggaaaa taaaattcat tgcactacgg
     1141 gaagagtaat tactttagtg ttgtttttgt tccttcttct ttttgcgagg ggaactgcag
     1201 tccaggcctt agaaaaagtt gatccgagtc aaatcaaaga aaataataat ctggtgcttt
     1261 atcatgatag tgattcattg agaaatatcg aagtaactgg gatcaatgga tttaagatac
     1321 tagatgagta ttcaactaaa aaacaaaaag tacttgcttg gccaaaagtc aaagatttga
     1381 aaagtacgtc aaaaatcgtt gcccattatc aaaaagtagg tacttatcta ggaaaagaaa
     1441 ttgattgtga cgtgactttt tctaattttg tagcgcacgc tggggttaac caagacggat
     1501 tagctaaaac aattaatggc aaaaagtatt ataattattt gctctttgat aaaaatattt
     1561 atcgaggatt tttcatgcag tcaatcaatg tcatgaatgc aaaactagaa tttaagtatg
     1621 caggaacaaa aacaaaagta gctttgagtg aaccacagcc agaaattcaa tcaaaaatga
     1681 gatcgatgag agcagctggt attgatttaa acgatacatt tctatccatt tacagtttaa
     1741 atggacagta tcagcaacgt gtgtcttggt ataatgacaa taatgaatct gtcggtgaac
     1801 gtaatattga tatgagagaa tttgttgggt atgaaaaaat gggtagctta ccttattttg
     1861 tcacaacaga tacagcatgt gcagaataca aagctcctgc gttatcaaca aacaatttaa
     1921 cttcaaaagt agtgggagga cgtgcagaaa aggcttatag ctcgaatgat catttcaccg
     1981 atgttgtagg agctgatact tatcacagaa gtggtgtaac gtatacgctt caaggcgctt
     2041 ccccaacatt catgattggc gcaaatacga atagtatgat gtttagcttt gatactgcat
     2101 tgctatggac accacaacca tcgaagccta caaaagaagt gtttaacaaa gctaatactg
     2161 aagaggcagc acacaatatt gacaaaaaag tgattccaca aggatcagat gtttactatc
     2221 atattcacca aaagtttgat gcattaacag tcaacacaat gaacaaatac aaatcattta
     2281 aaatcactga tacctttgac agcaaaaatt ttgatatggt atcggatggg aaaaactatg
     2341 atggcgcatt gtggtatgtt gaagcgaatg gtacttggaa aaaagctgat acaggaggaa
     2401 agttaactgt atcaggtaat acagtcactt atactgcttc tgacagcttt ttagctaacg
     2461 ctgcaaatta cggtagggat tatattttgc agctccatat gagagcaaaa aatgatgcaa
     2521 aaggaattgc accaaatgtg gctactgtaa cgtttaacaa tgatccgaac aaaggaacac
     2581 aaaaaacaaa tacagtagaa aactatttct ctcttaaacc aaataaaaaa gtggaacaag
     2641 atgggaaaga tgttgatggt cgaaatgatg gtaaaaaagg aacgcctaca gctcctttaa
     2701 atgcaggatc tgaagtacaa tatctagtca cacaaaaatg gcatacaaaa ggtgtagaca
     2761 ttgcctcaga tcattacaag caatttagta ttcaagatcc agtggaagat cgtttgacgt
     2821 ataaagaagg ttctgcgcga gtgatagata tgagtacagg aaaagatatt acttcagaag
     2881 gtacactaac ctatgatagc aattcaagaa cgctgaagtg ggaagcttcc gctgatttct
     2941 taagtaaaaa tcttttagat ggacgagaaa ttcagctgat atttacagct aaaactcctt
     3001 tgaatgaaga attggatatc gacaaccaag caaaagtttc agtagaaagt attgagaaag
     3061 caactaatgt agtaacgatt ggggtaaaac ctaacttacc tcagatcatt gtgccaaaaa
     3121 ctggatcgaa tcatttagtg ctaactgtta ttggaagtat attatttctt actttagcat
     3181 cattgggcta tgtagtttta aaactcaaat aaatataaaa acaaggctga gaatcagcct
     3241 tgttttcttt tatttgaaga ttggattcgc actaataaat acattatcta cgccaattct
     3301 accagcagag tcaactacaa aattatgacc taatccgaga atactttggt tattataaag
     3361 taaatttgca ttatgggcag ggctattttt ccatcctacc atggcatcga tagcaccatc
     3421 gtctgataaa aggtcatcaa tatacagccc agtttgactt atgttttctc ccttagcacc
     3481 gtagcagcct aagctttcca ataaatcttg gaaagtacca ccaccagggc gatagtgact
     3541 gaagctgact tgtaattctt tagctcgaat atctgcagcc tgttgaacaa tatcattgaa
     3601 ttgcattggg gcaacaccat tttgttggcg ataatcattt aaccaagcaa gttctttttg
     3661 acgcataaag ttacgtaatt cgtctaatga aatcttttta tatttgaatg tgattgtttt
     3721 attttgatcg acgttattaa tggatcgttg gccgttttta tccacggtaa cggtaggatc
     3781 gtcatttctg cacaagaaat aattttgatc agtcatgttt ttccaaggag tagcaaagct
     3841 atccccttcg tatactgttt ttgattcact agagagaaca gcgttggtat cagcatttac
     3901 atgattaaca gtcacattaa atttacgagt gtagttgaaa atataatttt gactttcagt
     3961 cacattgttt aaggttactg aatttgttgg ttctacccga tcgtctaaaa catatccttg
     4021 aatagaagga gcagttaaag tgacactgtc accgtgagtg acattgacta tctgttcttt
     4081 caaaatatta actcgtcctt cgtttttgta gttgttattg cacaacaaat tgatttgatg
     4141 cacttgatta taattgaatg tgattgttgt attacctgtg attgttttgc tttgagtatc
     4201 attaccaatc actttgtagc taaaattatc tttagcgcct tcgctatatt taaatgcttt
     4261 tgccttagca gtgtaagtag aaccagaagt tacttttgct tgatctgtag caatcacttg
     4321 acctgtttga ttgtcaatat ggttaatagt aaccgtatat tcttttggtg tttcaggttt
     4381 gtgttgcaac cctttaaatg cagcatcaag actattttta gcattgttta cttgtgtttg
     4441 agaaacctta gaatcgtcta gtactttttt agcattggat aaagcagtct gaaaattatt
     4501 ccaagattct tctgtaaaat ctcctttagc agttgatttt actcggttgt ataaagcttg
     4561 taattcagct ttattcacag taggaactgg ttttttcttt aatcctttgt aagcagaatc
     4621 taatttattt ttggcactat ccacttgctt ttgagtcgct tttgagtcat ttaacacttt
     4681 cttagcattg tttaaagcgg tttggaatgc gttccaggta ttgtcggtat agtcaccttt
     4741 tttcgttccc ttaacgctcg tataaagagc ttctagagcc gatttattca ctttgggttt
     4801 agaggtgtca gcgccatacc aaccgatacc ttcgtatttc cagccatagt ctttggttaa
     4861 ccattgtact tctttccaac ttgcagtgta gtgatgtgag ccagatttta caccaggaag
     4921 gaaaaggcgg tagatcggtt tattattttt ttcggcagaa cgccaggcag gcccttcata
     4981 agtccagccg taatttttag tcaaccattt cacttcatcc caactagctg tgtaatggtg
     5041 atcacgaaga cctttattgt acaaacgata gacaagatgg ccaccgctag tcggtgcttc
     5101 ccaggcgggg ccttcataat ttccccattt tatctttact agatggtcac gttctttttt
     5161 gctagaggtg taaaaatgtt cattattatt tggattgtaa aggcgataca tgacgttagt
     5221 tgaagcagcg tcaacagatc cagcaagact aagtgaggca gcaaatgttc caatagctaa
     5281 aacagttttt ggattaaata atttcatttt ttcaactcct aaatttaatt aaaaatgttt
     5341 acatgattat tatatatcgg ctaaccgata tagtcaataa aaaataagaa aagccttata
     5401 taattttggc ttttcttatt ttatttgtta gttgaagtat taatatcaac gatttcttca
     5461 gccaatttat ttaatttcat agcatttatg aatctcatgt tgtctatact agagcgtttg
     5521 ttggcaaggt cacttactgt agttaagggt acgccagatt ctatggatat agaacgaact
     5581 gatttaccac tatcaaacag caaccattcg attttctttt tatcagcgta catttcaatg
     5641 ctccttcttg ttttttcttt attatagctc ttttttgtaa tgaatcacaa tatcaagaga
     5701 aaaattttct atagaaaggt ggatttttat gagtattcgt tctccgtgta tccaaatgag
     5761 gtttgttgct actttcgtta tttgatatac taaaaataaa caacgaaaga aggaataaaa
     5821 tgaagaaaaa gcaactatgg tttgtctcta tgtttgcaat attttttatt attttaagtg
     5881 gatgttcaag taataagaaa tcggaagcta aagaagataa tagtttattg caaccaggaa
     5941 ctgtttggaa agaagaagta ggtggtctta ttcataattt aaaaattata gatgatacta
     6001 cctgggaata ttctgaaagt gtatggcatc cagatcctgt acaaataaca gttaagaggc
     6061 aaaaagacta tgaaggttta gaaagataca aaatagtaga ttctgcaggc attagggaat
     6121 ttataaacaa acctgattca ttatttattg ttgttcccta tgagaaggat ggcgtaaaaa
     6181 aaataatttt tcttggatct agcaaggatg agaagcgaac gaaagagaaa ttaattcatg
     6241 atggatcaga gttaaataaa tacaaattac aaaagactag tgagtgattg ctagtctttt
     6301 tatttatcct gaaggaggaa tcacagtggc aaaagttaaa acaccattcg aaaaagccgc
     6361 gtctaaaatc ttagaacgcc gtggtgtttc tgaaatacag tggaaaaaag aagttcttga
     6421 aaaagcacaa tttgacctat tacgaggaaa agataaggac ttagaagaat atatctatca
     6481 acgtgaacgt gaaaaacttg tattcgatga aattattaaa cattaaaagg agcgaataat
     6541 cttatgtttg aatcaattac aatgaatttt ttaactcaac cgaaagtagt agctgcagtt
     6601 gatattttta gagcattaca aactgccgca actagtttga aaaataatct gatgattttt
     6661 gctgctgttg cggcagtggc ttccattgct cttggaggta ttttcttctt ctttgggcga
     6721 agaggagctg aaaacgggaa gacacacgta ggttatgtat tgttgggggt aggagttatt
     6781 ttagctgccg cttctattgt agtttcaatg ttctcaatgt ttggacaaac cccacaagga
     6841 ctataaaaaa aacaagtagg aggagtaaaa agtacgtggc tactttttac tccttttttt
     6901 atgaaaggag aatattcatg ttttttacga tttcaggaaa tgcaaaattg gataaagagt
     6961 taataagaaa agcaacgaag aaagtgggaa agcgtacttc ctttttaccg aaaacaatta
     7021 aatattctgt cattgaaacg gaagaacagc tacatgtgtt aggacaagtg ttagaacaag
     7081 agaatcaatc ttctgttaaa ttgactctca cgttacatga taaagaagga aaaatagaaa
     7141 aggctggcac agaatacgtt tttggcccac tggattatca aatttcagaa ggtttttatc
     7201 ctacattgat agatttaatc cgccttgatg tgtttaaaga tacggatgaa actatgagtg
     7261 tggaagagtc ttatcgaaac gaaaaacttt tacaagtttt agaagaagag ttagagagcg
     7321 accaaagaaa agtgaatcaa gtgagtgaag tcgaagtaaa tgaagaagta ccatgggaac
     7381 gaccaatctt tccaaagaag gagtcaaaag aaaaagagac agtctatgtt gacgaacaag
     7441 ctgatattcc gactcagaaa tcgatatttg aggaagagaa cgatgtgatg ggtcaattgg
     7501 aggtagatcc ctttgaagaa acaatggagg atgaagagac ggacgaagta gaagttgatc
     7561 cagttgaagc taatgaagag tcaacatgga ctgaagggga ttttcaggaa tatattgaac
     7621 cacttatcag tgaagaagta gaaaaaaacg aaacagagat acaaaaggca actgtatttg
     7681 attatatttt ggatcaatat ccgccagagc atgaatggat gaaagaaaaa aacaaattat
     7741 ttttaaatga gctttattcg aaatatcaat taccccaaac ctatcaagca tttttagaga
     7801 gtaaagaaga attaatctca cagggtaaag gcacactttt agaacagtta gagtctgtcc
     7861 aatcaacaaa ttggcatgag cttgcacaag aagatttatt agccaccttt gaagaacgtc
     7921 aacaagaaac agatcaagaa attaatgagt atgcgacgga acagtcgtct aattgggaga
     7981 aagtaaaaga ggaactaaat aaagaagaac agcgagtgat tgaagaagaa gtacaaaaag
     8041 tcaaagagcg gtacgaaaag aaacgcgaca atgcttattc cttgtgtgta gaaaagatca
     8101 acaattttac taagacgaat caagaagcgt tatcgaaaga aaaagaaaac ttgttaaaag
     8161 aacgtattaa agaacgaaaa gaagagttct accgcggttt acgtacagat aagctaaaga
     8221 ttagcaaaga attaaatgaa caactgaata agctatatca aacaacgact gaagttctta
     8281 tggaaaaaca cgaacagatc cagcaagaat tacaagtaca aattccaaaa tggaaaaaag
     8341 agtatgaaat tcaacaacaa gaattgaaag aacaagaaga aaaacaacgg caaaaagaaa
     8401 aagagcgtga acaattagaa ataagaaaac gggaacagga tcttgcggaa caaaaaatgg
     8461 cgatagaaaa acaacggtta gagcaagaaa aggaaaaaga agcagctgct cgtaaggaac
     8521 gtcaagaaca aatggataca ttgcgtcaag cacagttgaa ttatctagca caacctcaac
     8581 cagtagctgc tgttcaaacg ccagtgaaca accaagcaaa tttaactcct tcaccccagc
     8641 gttcctttaa cggatggatg gtgggatgta ttgcaagttt aagcttgctt ctaggtggag
     8701 gaacagtatt tgcttttaat catatcaatc aatcttcaca ggctgattct gtagccacaa
     8761 cggcgtcaat caaggaagaa gcccaggaac aagcaagaaa agagtatgaa caacaattag
     8821 aagaattatt gaatcgtacc aatcagtcaa caacagagtc aagcatggaa aatagcaaag
     8881 agagtgattc tagtaaggct aaagaaggat caagcgattc aactgtacaa tcaaacactt
     8941 ccgaaagttc agaatcaaat gaggtgaaat aatgcgtgtt agagaaacaa cgaagcgaaa
     9001 agacaaacga ttcaatttag aaaagaatag gtatctttct catttctatc gctatactcc
     9061 ctcaggagaa gagctaccag aaaaacatga caaacgcatt tcgaaaaaga ttttttatag
     9121 tggacttgta tacctccctt tagagtattt agtaacaaga gtaccagaag ttttcaaccg
     9181 attggaaatt gttaagggaa gcttacaagg aataaatctg tttcaattac aggaaatgcc
     9241 acaggtcttt cattttgatt cagctggttt ttggaatcca tttactccta caactagttt
     9301 gggtacacgt ttagcaattt cgacagtttg tgtggttcca gctgtactta tgggattgaa
     9361 aatggacatg tggtttcgtc ctctagcaga tggacaaaaa ggggataatc gattaatgac
     9421 cttaaaagaa atccaacaga tttatccgca aatacctgaa acaaaaacta cttttccagg
     9481 ttatggaggt attccaatca cacattacaa aaaatattgg tacattcaaa cagatactgt
     9541 taatacgtgt attgttggta cctctcgatc agggaaaggt cagattgaag tgttagccac
     9601 cattgacaat ttatctcgtg cagaaaaaca aagttcaatg gtcgttaatg atccaaaaac
     9661 agaattatat gttggtagta aagatgaact agaagctaga gggtatgatg tatatgcctt
     9721 taatatcacc gatccactgc aatcaatgag tgataatcca ttggcactca tcgtgaaata
     9781 ctggaaacga ggagacatag atactgcaac acagttaacc aatactttca caaatacgat
     9841 ttattatgat gccaacgctt cagataacaa atactttaac gaaaacgcgc agaaagctgt
     9901 caatgcgctt atttttgcgt tgttagaaca agctgatcaa acaggagatt actccaaatt
     9961 gacaccgaat aatctcgttg agcttctttc tgagattggc ggttttaatt attatccaga
    10021 tccagacaat gaattcaaac agttgaatgc cttagatgaa tttatgaatc agttgccacc
    10081 tggaaacatt gcaaaaaaac aatatggggc aacgaaaatt ggatctgata aagcgaaggg
    10141 aaatatttta tcaacggcaa tcacgggatt gaatccattt actctaacga aaattgcaaa
    10201 gatgacttct caatcaacga ttgattataa atcggttggg tttccaaaat acttagattt
    10261 gaaattacat gaaagtcttt taaatcaacg agtcgtagta gaattttatc ataagggaaa
    10321 gaaatttcat gaagaacgtg tcaaagtagg ctataaagga tttagtgaaa tcaattttga
    10381 ttgtcattta gttgcaggtg atcaagtttg tttaaaatac gatcatcaag gaaaagaata
    10441 tgtggcttgg tatgaatttt ctcaaagagt attgaaagac gaagagggta atatcgttta
    10501 tcggaaaaaa cgaggagaaa cccatttgcc agaattagaa aaagaagccg ttctaacatt
    10561 agatgaggaa aaaagtaact tattagtaga gtctattcgg atgcactata ccgataaacc
    10621 aatggctatt tttatgctta cacctgatta tgatccatct aaccatatca ttgttagtat
    10681 ttttcttagt cagttatata aagaattatc tacgcaatgt gttaagacta aaggagataa
    10741 atgtcaccgc aggattcatt ttattttaga tgaatttggg aatatgccac caatggataa
    10801 tatggaagga atcatgacgg tcacagctgg ccgaaacatg ttgtgggatc tgtttattca
    10861 atcttatcag caactttatg cgaagtatgg agaacaaaac gcagcgatta tcaaagaaaa
    10921 ttgtcagaca catatttata ttatgtcgac agatgataat accatcgaag aattttctaa
    10981 aaaagtgggg aataagacgg ttgaacaaga gaacagtacc atcaattcat taggtatgaa
    11041 tactcatatc aatcgcaatg ttgattcaga tcgtatctta actcctgaaa gaatttctac
    11101 ttttttagaa ggagaaacga ttgtactcgc tcctttaaaa cgacaagatt tgaaaggtca
    11161 taaaatacgg ccgtatccta tttttaatca tggggaaacg aatcttcctt atgcttatca
    11221 atatattgaa gatttcaatc catcgtggga tatcaatgat atagacatac attgtacaca
    11281 tgcggacttg gatcttaagg ctttacaaat tgattatgat tatttcctac cagatgaata
    11341 tgctagaaat ctctatcatg gactgcatga tcaattagaa gaaaaaaacg aagaaaatat
    11401 gttttataaa atcgttcaat ctcttgagaa tgagacggct aaaaaattgt taattcaatt
    11461 ttacgaagaa caagcagcag atcgacttca agaatatgtt gaaaagttga tcaaaaataa
    11521 agaaatttct gaaatgattg ggaaacggtt gttattggaa tctgaaaagg tggtgaaaga
    11581 atgatcattg gaggaatttt ttctggcata gccgactggt ggtcaggctt attcggagaa
    11641 ggggatgcaa accccaaaac cgtaacgaaa gtcttacaag attatgggaa ttatctgcag
    11701 tatcaagatg tatttagttg gatttggaac acttttctgt ggggattgat taaaggatta
    11761 tataatctca atactttact agaaaaaacc atttatcaaa gctttgattt aaaagactta
    11821 ttgaacgccg ctggggtaaa tgcactattt caaagtttga ttagtaaggt attggctctt
    11881 ttttgtgtcg tcacattaat ttatttaggc ttgaaatttt ttatgagtaa acacccacca
    11941 aaagtcaaaa atatcttggt taatttattt atggccatga tcttgatttt tggtggtagt
    12001 tctttaatcg atgaagggtt aaatatcagt aaaagctttt acggagacgt cactgcagca
    12061 aataaagcag ataaaacggg atcacctgct tttcaaatta ttcaaaataa tgtgatcgat
    12121 gtcagtacgt tactagcaac tgacccgaat aaagtagaat ccctaccaac agataaaaga
    12181 aatgctttaa cagcgaaaaa ttttaaaaca gcgaatatca atgggattat tactcctgaa
    12241 caagctaaag gtatggcagg taatgataaa agtgggttat cagatataca aaaggatcgg
    12301 ttaaaggcat tacaataccg attggaacta gatgataatg ggaaagaaat tcctgttcaa
    12361 attagtgacg aaggcttagc aaaatatgtc tatacttcag ggtatcgtcg atacagtagt
    12421 aaaccaggta ttattttagc tggtgaatct tctttggcag tagcctattt atttatccta
    12481 tttacgatcg ttacttgcat tattgagctg gtatttaaaa aattctattt agtgattgcc
    12541 gcgtcaacgg actttgagac aggtcaacgg atgaaaagtg cgatagaaga tatttctcaa
    12601 tccttcttgt tattggcgtt tacgatttta gaattacgaa tctatacctt aatgctttca
    12661 ggtatcggtg atttgcatgc ggcaggaaag atcaatggat tcttgtatgt cgttgctttg
    12721 attgtactaa ccattgcgct attcaaaggc tctcaatcag ttacgaaaat tttcggcgtt
    12781 gatacaagcc tgaaaaacgg cagtaattct cttcttagca tgtttgcctt aggaaatatt
    12841 gcgaaaaatg tgggtagcgg agctaaaaac cttgcaagtg ctggtaagag tggtttaagc
    12901 catttaaatg atattcgtaa aaatggattt ggccatcgaa aagatgttac ccctgataac
    12961 ggggaagaac aatcagacga taaagctaca actgtaccta atagtaagaa aacagtttcc
    13021 gaaatgttcg ggaaagcgaa aaatggtgtt tctaaagccg cagaaggtct tggttacgtc
    13081 aatgaacgtg ggttaaaagg aacagctgaa gatcttacag aaaaagccaa agacacctta
    13141 gaagataaat tagacggcac gagagctaaa gaattggctg aagctgtgaa gaatccatcc
    13201 gaagcactag aaaaacaaaa agaaaaagct tcagaagtca aagaaggact aaaagatgcg
    13261 tatggtgaag gacaagtcaa tgcagcagtg aaagctaata caacaaccga aaaagaaaat
    13321 caagaaggta tagtgattcc ttcagtcaaa gatcaccaag aagcgttgca ctctaatgaa
    13381 aattcgattg aagagaaaaa ttccatagaa gaaagaaatt cagaagaagg aaaaaatgga
    13441 cagttaccaa aaactgatcc agaacaatat cgctataaac ctaacagtga ccttgaaaat
    13501 gagaagcgaa tttcagattt atcaaaagta aatcaagaag acaaagcaga aaatgaggga
    13561 cagttaccaa aaactgatcc agaacaatat cggtatagat ctgttaactc cgaaaatgaa
    13621 aaacgcattt cagatttacc aaaacgaaat caagaagtac aaacggatga ggattccatc
    13681 gttctgccaa atgataatgg aatcgaagtt ggaactgaaa aatcaacgaa aattggttta
    13741 cctaaggcac aaaatttacc aatgaaagat agtcgtttga atcaattcaa ggataatgat
    13801 caaagttttg ttcatcaatc acctgcaggt actgttcaaa tgaaaccaag tcgtttgcct
    13861 gaaaatggaa atctgacaac tgtttcttac aatttatctg aaagtggtga gacagggaat
    13921 gcgtccgttc aaaatgttgt gacaagagaa gctgcaccaa aacctattca aaacgttcat
    13981 acaggttaca agccagcaaa tgtggcattt actgcaccaa caaaaataaa tgtgacaaga
    14041 gaaacaaaaa acaggaacaa tgcattgaaa gaacaactat tgagggatat caaacaggat
    14101 atcaaaccat aaaaggccta caagttaggc cttttttcta tgtatgaaaa ggagaaatca
    14161 tttatggata aatatagtaa catgaaaaac cctccaagat ttagtggaga atggaagttt
    14221 ttcgcttttt tctttttaga tctattaacg cttggtggct gctttttttg ggcgaaaatg
    14281 gtcaataatc ttgtccactt accactcttt tttgcactct tgaattatgg cttatggatt
    14341 gcgatagcgt tagtgattgt ttggcgaccc tataataacc caggtcaacg acaaattttt
    14401 gcgattatcg agtctatttt ttttgcggac aagaaccatt atcatgcgtt agatcctaac
    14461 cgaaattatc atggtacgta taaggaggat gaataaatgc gagtcaaagg acaaaattca
    14521 gttgaaagga aatccatttt gcagtttgga aaaccagaag agcgctctat cttggactta
    14581 tttccatata aatcctgtac agaagaggga gtcttaatta ctagagaaga tcattttcaa
    14641 cgttattatc gtgtagtttc tacagacgtg gaaggattaa atgaacaaga gaaactagaa
    14701 cgaatgaatc agttgaccac cgtcatgcgt acgtatgtgc caacaattaa gttagttagt
    14761 ttgaccacag agacggatct aacggaacaa atcgtgcaaa agcggcaatt attagataaa
    14821 aatcgtatgg aacagtcctt aggaagaaat ctacaacgat taaaaaaata tgaaaagaaa
    14881 ttagtcgaag aaatccagga attgaaacga gcagaacaag aacgtcctga cttaagcttc
    14941 tttttcgtca ttgaaggaaa gacaatcaag gatttacaaa ccagaaaccg tcagttaatg
    15001 cgatctagtg gcattttagg gttaaaacca ctctacaaaa aagaattgat caagattctg
    15061 tatcgaatga acaatatgaa tgatgagtag gaatggagaa gagagaatga aatatccgtt
    15121 gatatggtta gaaaaagatg ttccgaagga agagaccact agaaaatttc ctcacacttt
    15181 aagcctgggt aagttgggaa aagggcaatc ttttaaccaa gtaaagaatc aaacaagaag
    15241 aggtgactgt acatgttaga agcaattaaa acagcttttt caccacaaaa tgatcaaaaa
    15301 gtacttgatc cattaaagca actaggaaat cgggaaatag aaattgtaaa atccaaaggg
    15361 tatgatttag attttttaaa acaagtgatg ccacaaggtg ggttaaattt tgatgaagaa
    15421 tatgtgacgt atggtaatgg ttattcacga tgtgtgacag tttatcacta cacaaaaaat
    15481 cctacgcttt tttggttggc caatctcatg aacaatcctc atacagtggc cacactcgat
    15541 gttagcacag atgaaaagca aaaagtgatt gatcaaatca atcgtgcctt agatgaatta
    15601 aatgatcgat cagataatga acgaaaagcg actgaccgaa ataaaagcgc ggaagaatac
    15661 ctggaactag cggaatatgc tagaaaattg acttctaatg gggaaatttc taagcaagtc
    15721 aaagtgagag tacatgttta cggaaacacg attcaagaag tagatgaacg agcgcaggaa
    15781 ctgatcgaaa atctcaaagg gatggattat cgtgcggtga cttatctgca taaaaccaaa
    15841 cgagattggc aatcgatgtt taccatgttc gatgaacaag ctgaatggtt cggaaatatc
    15901 aaagggcaat cactaccatc gttaaacatt ggcggaggtt tcccgttcca ccaccaaacg
    15961 ctaaaagatc caggagggac acaatatggg gaaaccatga caggcggccc atttgtctgg
    16021 aatgcgacgt ataaaaatag tgtacggttg tctactaata ccttgcttct aggaatgatg
    16081 ggagctggta aatcgaccgt tcttaaaatg atcgcggaag cccatttagc tgcaggagac
    16141 tttatttggg gctttgaaaa gggaaaagac tttattcctt tcttaaagga atataacggt
    16201 attatggtgc gtttagacgg atctgacgga atgatcaatc ctttagagat atttgccaca
    16261 cggacgtatg atgaagcatc cagtttatat gacgatggat cagtcaaaga tttgaagatc
    16321 aatgaagcag cgtcctatca aacacactta gacaaagtgg tctatcaggt acagttagtg
    16381 agtcctcagc taaaaggaac gatgaaagca gaatttaaga cctacttaaa tcgtttttat
    16441 gaagagtatg gaacagtacc acgcggcttt acttcctcaa attcgcgttc aaatactgat
    16501 acgcaagtca cagggaaaga tccagaagct tatccgacgt tcaaagaatt tttagacttt
    16561 ttaggacaac tggaattacc aggtgcttcc caagagaaaa agaatcgaaa agaagaaatg
    16621 gaatccatcg tagaatcctt atgtgagacg tatggcatga tttttgatgg tcatagtacg
    16681 attcgacacc ttgaccaaca acagttggtc ttttttgaca ttgactctat tacaggctta
    16741 gatcgagggg tacaacaatg tcaaatgtac atggctatga cgttgatttg gaatcaggca
    16801 ttgattcaag gaagaagaca acgacgttta attaaagaaa aacaaatgga agaacaagac
    16861 ttcaaacgct tcttggcgat gattgatgag tgccataatg gtgcgacctg ttctgcagtg
    16921 aaaagctgtg ggttgcaagt agtctaaaaa ctattgtaat aactcttata acgagaagtc
    16981 aaatgagtgg gtaacgccac aaagggcttc cactgatact ccgacatgca acaagtagtt
    17041 tatgcagacc aacgttgtgt gaagctcggt gaagtcggct gaaggatacc gtaaggtaaa
    17101 agaaatagtg aataccacga aagttctcta gaggtaaagg gtgtatccga taagtcgggt
    17161 gtccaaaaaa acttaatatg gtgagtatgt acaaacgtac tgacgaacta gcgaatgtac
    17221 tgtccgaaag gcatagctcc tagaaatggg agagacagtg agcaaaaaat cactcattgt
    17281 gctaacttgt ggttgagtaa aagtcgttgt tatgaaagac catgctgcgt tacaggcgta
    17341 ggagaagtta gggggcaaaa gctagcatct aagttaaggc atgtggatag atataaggga
    17401 acaggggaag ccatcaacat ggagagtgca tactcgatga agtggtagta agaaatatgt
    17461 gaatgacaaa taacttactt tagtgatggt gaaagtagag tcacgactga tgaaggttct
    17521 gtaatggaac tggaggaaca ggctcgattc gtctgtaaaa gtcatgaaac ccgttcaacc
    17581 atgggattct cgtatgacta aaaaggtcac gagccaattg gagcgtgata aaccatgaca
    17641 aaagaaacaa gtccaaaaga taggaagttg cggcatgcag aatattatgg catgacggaa
    17701 atcttcgata agatatactc caaaagtttg aagggagata atttcaagaa tctaatgcag
    17761 attatcattt ctgataacaa cattgtccta gcatatcgta atatcaagag aaatcacggt
    17821 agtgtgacaa gaggagaaga tggagtaacg attaaagata ttgaaaatct caaccgtaat
    17881 gaattcgtag aaattgttag gaagcgattc gcacattata accctcggaa agtaaggcgt
    17941 gtagaaattc ctaaatataa tggtaaatta agaccgttgg gtattccatc aatatgggat
    18001 agaatcgcgc agcaatgcat cttacaagta ttagaaccaa tatgtgaagc aaaattcaat
    18061 aatcacagct atggtttccg cccaaataga tcagcagaac acgcaatagc agattgtatg
    18121 gcccgaatga atcaaagtca catggaatat gtggtagatg tagatatcaa aggtttcttc
    18181 gacgaagtca atcatactaa actcatgaga caaatctggt ctatgggtat acgagacaaa
    18241 cagcttctct taatcattag gaaaatgtta agagcacccg tagtacttcc aaatgggaag
    18301 ttgatatacc caacaaaagg aacaccacaa ggcggaatac ttagcccatt acttgcgaac
    18361 atcaatctca acgaatttga ctggtggata gcaaatcagt gggaggaaaa agatgccaaa
    18421 gagctaaaac atcattttag gaaaaatgga gcttgggata aaggtaatat ctatcacaag
    18481 ctcagaaaga gtacaactct aaaagaaata tacctagtga gatacgcaga tgattttaaa
    18541 attttctgta gaaattataa tgaagcgaaa aaagttttct atgcaactaa aatgtggtta
    18601 caggagcgtc taaagttacc aatatctgaa gaaaagtcaa aaatcacaaa tctttcaaaa
    18661 gaagaaagtg aatttttagg ctttacgatg aaattagtga ccaaaggtaa caaaaaggtt
    18721 gttaatacgc atataggtac aaaagcacaa agcatgatta aagtgaaact aaaaaaacaa
    18781 atcaaacgaa ttcaaaaaag tcctaatagc aaagatacct ttatctatat tggagtctat
    18841 aacagtatgg ttattgggat tcataattac ttcaaaattg cgactcattg tagtgtagca
    18901 atgcgaaaaa ttgcaagaga actcagacca agttttgaaa tacgactacg agaacaagga
    18961 atctcaaaag aaggacaata cggaaagtac gaaggataca agccttacct acggtcagga
    19021 caaatacgat atataagagg gtttccaata ttaccagtag ggtatattca acacagacat
    19081 ccgatgtgta aaaaaagaaa tattaataaa tacaccgaag aaggtaggga agccattcat
    19141 aagaagcaac aaaccgtaga agaatggaag atatcgtggt tgtgtagcaa tccagtgctt
    19201 agtaagagag caacgattga atttgcggac aatcgaattt caaaattcat cgctcaaaag
    19261 ggaagatgtg cagtaacagg tgaggagctt atcttatctg aaatgcattg tcatcatatt
    19321 atcccttatc atgaaagcaa aagtgatagc tatgaaaacc tagtgattgt tactgaggag
    19381 gttcatcggg taatacacgc tacacaaagt gaaacgattg aggaacttct aaaatatcta
    19441 aaattaaatc caaaacaaaa agaaaaactc aatgagttga gattaaaagt tggtaacgaa
    19501 gaaatatctt aataaatatt tgtaggacgg gaaaacagaa tgtcttttat agacgaggga
    19561 acgccgtatg cggtgaaagt cgcacgtacg gtgtggactg ggggaaaaga cggagataat
    19621 atcaaactct tacctatcag tatcgattaa tccagatttt attgaaacgg taaattatgt
    19681 cacaaacttt caacgagaaa tgcgtaaagt agaagcaatg actgttttag ctacacaatc
    19741 cccacaagaa atggtgccag aaaatatgtc ttctgataac ttctcgaaac tgaagaaagt
    19801 ctttgaattt tcttctacga agatctttat gcgtatggat gaatctattt tacgtcatat
    19861 agaaggatta gtgggtaaat caatgacgaa ctcagaatta gaatccattc ctcaacaaaa
    19921 tcaaggagac gctgtgatca attttggctc aaaagaaacc attcgtgttc attttagtcc
    19981 gaacaaacgt caattaaaat tgtttgatgg aggaaaataa tggaaaacca aaatgaaagt
    20041 ttaatcaagc agtatgttaa gcgtcgtgca aagcggcgct tatttttgtg gttgtttggt
    20101 acttctgcag gtttgattac tattttgatc actgtgttcg tgaccctatt tctaatactt
    20161 gcggcgggct cgattgataa ttcggattca gattcaagca gtggtggcga agcttttaca
    20221 ggagaatatt cggaaggctt accgatttat aaagaaatca aaggaagagg ccctttttct
    20281 gatgagattg cgcaatatgc cgtaggtgca gcagtgaaat ataaattatt accaagtgtg
    20341 atcctttcgc aatacggtta cgaatcagcg tttggtacat cggcgtccgc aagaaatgat
    20401 ctcaactatt ttgggattac ttggtttgat ggttgtctgt ttcctaaagg aactgcacga
    20461 ggaattggtg gcattgaagg tggctggtac atgaagtttc ccaactcaaa agctgcattt
    20521 tcctattacg gctttatggt agccacacaa tccaatttta atgcttgtgt aggaaataaa
    20581 agccctggcg caagcttatt gatcttagga cgaggcggtt atgccgctgc aggaattacg
    20641 gaagatagtc cgtattatac aggttgtatg tcaattatca cttctaataa attaaccgaa
    20701 tatgatgaat tcgccatcaa acattggggt gaaggtggca ataacaacgg cacgattact
    20761 ggtgaatgga cgaatccatt ccctggcagc agtttagata aaagttcctt cagcggtggt
    20821 caattatttg gaacgaatcc aggcggtgaa ttccgtccga acggcttcca tgatggatta
    20881 gattttggtt cagttgatca tccaggaagt gagattcatg cggttcatgg cggaaaagtc
    20941 gtgtacgttg gcaatcctgg tatttcaggt ttgggcgcgt gtgtgatcgt gattaattac
    21001 gatggcttga atatggtgta tcaagaattt gcaaatagta caggaaattc tcgagtgaaa
    21061 gtaggcgatc aagtgaaagt tggtcaagta attgggatac gagatacagc acacttgcat
    21121 ttaggattta ctcgaatgga ttggcgtcaa gcacaaggac atgcattcat tgatgatggt
    21181 acatggatcg atccgttacc atttttgaat agcagtaaaa aatagatcag gagaaaaaat
    21241 atggaaaata aaacgttaaa aatcgtctta acagctgcaa ttagcgttag tttaatcggc
    21301 aatattgcgt tgttttggca gctgaaagag gaacaagcag caaataccta taaaattgaa
    21361 gaagtggcta aaaacgcgtc tgagaagctc aaaaaaaatt cctctgaggc acaggcgaag
    21421 atcgacaagt taaatcaaac catcaatgag aaaaaccaag aaatcgatca gctgaagcaa
    21481 caagatggca caaaagcaaa agaggaagta actgaagcac aaagaaagac ggcagaagag
    21541 ttcggtcaat tggccattga taaatcatta agcagaaatg aattaacgga aaaactaaaa
    21601 gatgtcgcaa cccaagaagt gatcgacaaa ttaagtccta cagacgatga tcaccaacat
    21661 gatgattacg gaagttactc ctttacatta ggtgacgtgc aaacttacgc tcaaacaagc
    21721 agtgtaaaag aggaacaaaa ttttgtcgta tttgttgatt atacaatcgg caatccgcaa
    21781 ttcaaagatg tcaaaccaca aaaaataaaa ggtggattaa ctgtcactga aaaacaagta
    21841 gatggtcaat ggaaagtcat agactttagt tatttcactc gttaggagga agaaaatgag
    21901 taagaaaaaa ggagaaagac aacgtttcaa aaaacgaaaa agttatctta gatggctggg
    21961 aatctttatg attctcggct ttttgattct tgctagtccg atttatacga atcagaaggt
    22021 agatatacca gatgaggtaa gcacgaacgc ctttttaaac acgaaagagc tgacaatggt
    22081 ttcaaaagaa aaaggagcga accagcaatt agtttttgaa tttgcgatcg atgatgaaga
    22141 tcaaagcttg ttaaaagaac tggctaatct taattatcgc gtagaagtaa agacagctaa
    22201 aggggattat caagcaatac gagccaaagt gattaaggtg tctgatgatt attttgcagt
    22261 gaagctaacg catgtaccag aagaatatat cgccatgcgt ttaaccatta ttcctgaaaa
    22321 aattgatcca aaagtggata tgcaagaacc acaagatctg atttattacg tgcacgaaga
    22381 taaggttaaa gatcaagtga aagatgaaga ttatgaacaa cacgcgatca attacaaagt
    22441 gaaaggatac aagaaagaac aaaaagcagc tcaaaaacaa atggaatctt tccaagcaac
    22501 cattgatttg aatgaagaat tagtgaagaa gctaaaagat caattgcctt atcaagtcgc
    22561 agatgatcag gaaaatactg aaaacaagat taatagctac cagcaagaaa ttgaaacatc
    22621 gaaaacgcaa ctgaaagatc aagaagagat cattcaaaaa cttcaagaaa aaattgatcg
    22681 tatgacaaaa gaaaagattt agaaggacag gtggagtgaa aaaatgaagc gaacaaactt
    22741 ggctgacaaa gatactttcg ctgagcagaa agtattgatt aaagcctatc tcaaagttgt
    22801 aaaagctaaa gaaatatgtc gagaagtgaa tttatctaac acaacaatat ctgagttgaa
    22861 gaatggtcga aagaatttag atactttaaa attcagatca tttgaagctc tatatgtagc
    22921 tgctaaaaaa tattttacag ctgataacaa ataaaaaaca ggagtatttt atgtgaaaaa
    22981 agtatataaa aaatgcttat gaatcagcta tatcacctta accatttgtt aatgagatgg
    23041 gagaaaacat taaccacgat tctaacgcaa acatcaagca cataaaacat ttaacactcc
    23101 tcctttctgg ctgaagattt caaccaaaca ggaagacagc gtatcaatta tagcatacac
    23161 tttttcttaa attcattttt catatgacaa aatatttcct tctaatcgac aaagttagaa
    23221 ggatttttta ttgtctaaat acacaaataa ccttaatttt taaagagcat aaggaaacgt
    23281 gaccccactt ttaaaaaaag tggttggata ggctgtgcct atcacgtttc tgaaaaaaat
    23341 aaatgacccc aaaataccaa tatataggat agtacaagga tagcaaagga tagtaaaagg
    23401 atagaggtag gatagcgaaa aggataggat ccttttcact atcctatatt atattggagg
    23461 atatttatga atgttaaaat tagagattta gatccaaaat tcatttctga aattgatcga
    23521 agatgcgttg aactaagtaa tcgtacagga aataaatgga gtcgtaatga ttatttaaag
    23581 ctactcgttg aaaatgattt tgatcgtcct ttgatggaat acaaacaaga aaagtttgac
    23641 cagttattag aaaagttttc agaaattcaa agttataaca caaagatatt agaagaatat
    23701 gttagccaaa ataatcgtat tatagaaatc ttgattgagc aaaatagagg aagtgaactt
    23761 tgatatggca aaggttatag acaaacacat ttactttcac caaacacata tcgatgtatt
    23821 ggatgaaact atttctactc atgcagaggt taaaaactat agtgaagctg ttcgcttttt
    23881 atgtatgaac tatgatccag aagtggagaa aaaagtaaat tccaatcaag caaagttgaa
    23941 tggaatgagt aaagaaattt ctattttaac cgaaatggtt tcagaatttg cggatcttca
    24001 tttgaaagat acaggaatat taattggtaa aaattcaact gtttatcaag aagcaaaaga
    24061 acgagtggaa ggaaaaatta aatccaatca aacaaaaaaa tattcccctc agttgaagaa
    24121 acctgtgaat gaatctgttc atgccagagt agatccattt gatccaacga agattctaaa
    24181 aagataaaaa tgaaggagga taataatgac tcaatcgcct tcagttattc tcacttgtca
    24241 atttactttg ccgaataaaa agtcattttc tacttatatt gactatatta caagggaaca
    24301 ggctcttgaa gaaaaggaac gacgtacgcc cgaagaagaa caggaacttc aacgaatcaa
    24361 gaatgcttta gaggagtttt atattcctga aggagaaact ttttcggaga gtaagacggg
    24421 taaagaattc agtgaaacgt cgaatgaagc tcaatcgttg atggaacatg gaatcaattt
    24481 tgacaagtta gaggaacaag attttaccaa ttatctttct tatatgacac gtcactatgc
    24541 gttagaacaa aagaaagaac tgacgcaaag tgaacaaaaa gaataccaac gcttgaacca
    24601 agcaatgaaa aaatatcaaa cagaacccca taaaaaagct tcaggaaatc ttcctggagt
    24661 tttttctata gcttcagacg aagtgaaagg aagtgatctg aaggagatta agcaaatctt
    24721 ccaaagagga caacaaaaag gctcaatcat ttatcaagat gttgtttctc atgataattc
    24781 gtatcttgaa aaaataggat tgtacgatcc caaaacagat actttagatg aagaagggtt
    24841 aaaagcagct ggaagaaaaa tgatggagac attattcgag aaagaacaat tgaatgaaac
    24901 aggttactgg atggcaacga ttcaccgtaa cactaaacat atccatattc attttgcagt
    24961 tgttgaaaaa gaaaatacgc gtaaacctta cacggaggtt gaaaatggtg tgagttatat
    25021 cgtaccgaaa ggtaagagaa gacaagcaac attagacgca atgaagacca attatgtaca
    25081 tgagttagaa cgtttttctt atgaaaagga tcgaatgatt ggtatggaga aacgaaattt
    25141 attgacgcgg aaaagtgatt tgcgtaacac cttaacaaaa gtggtaaaag agccagcgcg
    25201 ttacgatcga cacgcgatga acttattaaa tgaagtatat aaaaccctac ctgacaaacg
    25261 ttcggaatgg aattacgggg atgaaaaacg aaccaaactt tcaccataca caagagagaa
    25321 actcgatgag ttaaccaaat atgttttaac aaaagaacct gaatatcaag aatatataca
    25381 gatttcaaaa gcattgaaag aagaagcaaa aagtctttat ggggaaagca agagagaatc
    25441 aaaggatgca gagaaaaatg ctctctttga tttaaaaaag cgcacaggaa atgccatcct
    25501 aacagagctg aagagacagg atagtcaaat aaaaccatta atagaaagtg ttgatcagat
    25561 gcaatctatt tacaatgaac gatcctttcg aacgcttaaa caattgccaa cgaatgaata
    25621 caaatatagt tacgaagaat tcatgaagcg caggaaacaa ttccaacaaa gagttgtcac
    25681 gcagcggagt ttgcgtcaat taaatcgaac gattaatgcc catgaagaaa aataccacgc
    25741 aagtaaagca tacgaagagc aacaacgaag aattcaaatg gaacaagaaa gacagtaaag
    25801 acagtaggag gaatagcaat gaaacaatat ttagatcaat ggaaagttat agaaggatca
    25861 ttaagagaag aacgaatcga acaacttcct gattgtttag aaaaagagca tttgttccag
    25921 ataagagaaa tgttgagaaa cgaacagttt gatccaaatc aatttttagt cgttgagtac
    25981 ccagctactg gtgtgtattg ttgtaatcat gtgaatggtg aaaaatattt tatcattcaa
    26041 gaatatgagg gcaagttagc accttattat actacttggg aaatgaatga agagggaatc
    26101 aataactttc catgtaaaag ca
//
LOCUS       DACDIH010000033        25750 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDIH010000033 DACDIH010000000
VERSION     DACDIH010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.033"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(451..1761)
                     /locus_tag="IV281_001483"
     CDS             complement(451..1761)
                     /locus_tag="IV281_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8780479.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="IV281_001484"
     CDS             complement(1773..3191)
                     /locus_tag="IV281_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8780480.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3395..4963)
                     /locus_tag="IV281_001485"
     CDS             complement(3395..4963)
                     /locus_tag="IV281_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP8780481.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4967..5377)
                     /locus_tag="IV281_001486"
     CDS             complement(4967..5377)
                     /locus_tag="IV281_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP8780482.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5374..6297)
                     /locus_tag="IV281_001487"
     CDS             complement(5374..6297)
                     /locus_tag="IV281_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP8780483.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6294..7172)
                     /locus_tag="IV281_001488"
     CDS             complement(6294..7172)
                     /locus_tag="IV281_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8780484.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7159..8496)
                     /locus_tag="IV281_001489"
     CDS             complement(7159..8496)
                     /locus_tag="IV281_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8780485.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8499..9602)
                     /locus_tag="IV281_001490"
     CDS             complement(8499..9602)
                     /locus_tag="IV281_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8780486.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9676..11184)
                     /locus_tag="IV281_001491"
     CDS             complement(9676..11184)
                     /locus_tag="IV281_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8780487.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11150..11842)
                     /locus_tag="IV281_001492"
     CDS             complement(11150..11842)
                     /locus_tag="IV281_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8780488.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11864..12838)
                     /locus_tag="IV281_001493"
     CDS             complement(11864..12838)
                     /locus_tag="IV281_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8780489.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13138..14010
                     /locus_tag="IV281_001494"
     CDS             13138..14010
                     /locus_tag="IV281_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8780490.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14468..16666)
                     /locus_tag="IV281_001495"
     CDS             complement(14468..16666)
                     /locus_tag="IV281_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8780491.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16898..17890
                     /locus_tag="IV281_001496"
     CDS             16898..17890
                     /locus_tag="IV281_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8780492.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17950..19371
                     /locus_tag="IV281_001497"
     CDS             17950..19371
                     /locus_tag="IV281_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP8780493.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19364..20863
                     /locus_tag="IV281_001498"
     CDS             19364..20863
                     /locus_tag="IV281_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP8780494.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20902..22296
                     /gene="uxaC"
                     /locus_tag="IV281_001499"
     CDS             20902..22296
                     /gene="uxaC"
                     /locus_tag="IV281_001499"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8780495.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22455..23138
                     /locus_tag="IV281_001500"
     CDS             22455..23138
                     /locus_tag="IV281_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP8780496.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23140..23976
                     /locus_tag="IV281_001501"
     CDS             23140..23976
                     /locus_tag="IV281_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8780497.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23987..25741
                     /locus_tag="IV281_001502"
     CDS             23987..25741
                     /locus_tag="IV281_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8780498.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
BASE COUNT         7835 a         5236 c         4510 g         8169 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactat taaattacag atgatctttt ccatcatact
     3421 tgaaaaactc aaaatcagca taacttcctc tttttttatt tagatcatgc actgctatcc
     3481 cgataaaatt tccagtaaac cctcctgaga gtatacacat gtttttttgc ggacctactt
     3541 tcttccattc ttcagttgat acgtctttca aatgaaactg tgctgtctct ttctttacag
     3601 tgactttcaa aaaagtcttc ttttctgtga gtggaatcgt ccaatcttct atttgtgcta
     3661 ctccgttttc tgattctatc atacgtaggc attttccctt gtcctcattc caagtaacat
     3721 aacaataaat ataattggat tcatttaagt acagtaccag tccagccatc tgattaaaat
     3781 tattaggcat gtattcaaat gctgtctccg catcaaaagt aaaatccgtt tgccgaatag
     3841 ctaacaaatg atgttcgaag gtagattgaa gagattcacc ggaaatcaga cgtaaaaaac
     3901 ctggtctaca ttttgtatca caccaatcag catttggcaa aatgcgtctg gcattccagt
     3961 tcttctttag ctctccttca aatgtatcca taaactgatg atctttttct tgttgtacct
     4021 cttcatttgt ctcaatcatt atttcttcca tcggagttgt ctggctatct ttaagtctta
     4081 accaatcgtc ggacgtccaa tacacttctt gaatagcggt ttctcttccc aaaatagccg
     4141 caccgtttaa tggtctggta caaagatatg tcatgtacca tcttcctgac ggggtttgta
     4201 ctaagctccc atgtcctgta cattgcaatg tagaagcagg tttgtcacta gctgtcagca
     4261 taggaaaacc aggatcaagc tcgtaaggac cagtaatatt ctttgctcgg caaactgtaa
     4321 ctgaatgtcc tcttcctgta ccgccttctg ccgtcatcaa ataataatac ccgttacgaa
     4381 aatacaaatg tggtgcctcc gttttcgcta attctgtacc tgcaaatatt ttatagatgg
     4441 gaccatcaag cttcttcttc tctgcatcat attcttgtaa tacgataccg ctggatttat
     4501 tcggtgtgtc cagacgataa tcccatagtt cattgacaag ccattttttt cccgtttcaa
     4561 gatcatggaa caaagaagga tcaaagccgt tactatttaa ataaattggt tcggaccaag
     4621 gacctgtgat ctcagatgca gtgatcaaat aattgtggac atctttaaac ggacggcctg
     4681 cacttttgac atctgtgtaa acaagataaa acaatccatc agcataactg atctgtggcg
     4741 cccatatact tccatttgta ggatttcctt gcaaagatac ttgagtggtc aaaatatccg
     4801 ttgtatgtga ccagttgact aaatttttcg actcatatac tcggattcct ggaagccatt
     4861 caaaagagga gacaataatg taataccgct ctttcactct taatatatta ggatctggat
     4921 tgaatcctct taaaatagga tttttcactt tgatagtctg catgattcag gctccttttc
     4981 tcattcttac tcgatataaa ctgaatgccc cagctgcgaa ataaaaaaat gcaaaaggaa
     5041 acgcaataaa ctgagtgcca atcaacaata tgaatcctgt tataaaatac aaaccagtgg
     5101 cagttgttaa gtattttcct ttagcagtca aaaacaatgc tactgctaaa aaagcaagtg
     5161 aaataaacaa cacaacggca aacagaccag tcatgtcctg taataatcca aatgtatttt
     5221 ctaaagacaa agttgaatga tctacagcaa gcgactgata acttttttta aactctgacg
     5281 cagacatatt acttaaagta actgctaacc cgccaaagaa aatcaaagat agactgccac
     5341 cgaccattcc tagcaatagt tcccattttc ttttcatttc tgaaccccct gcttttctaa
     5401 atgtatccat tcaggtcttt tgatcgatgt ttcataattg ttattacgtt cagagaattt
     5461 gacagtctgt tcatttttct tgtcatccca attatcccaa ccagttgggt gaacatgccc
     5521 acctatctta cacgcttcaa ataagacatt tgcatacgga cgccacggtc ttcctaaata
     5581 gacgtttcta gtatcaggtt ctgcagtcaa ggtacagctt tcaaaacgat accctgtttt
     5641 ttgatttttc ggcgtagatg ctgctgtgat gtatccagga tttttactgg ctaagctatg
     5701 gatatgacac tgatgaaaaa cggcatctgc cccaccaaat atgaaatcca ccgttccttc
     5761 aatccagcaa tattcatagg tctgacgaca atactcagga ttggcagaag ccggtgtaca
     5821 aaaaggagta ccatcttttt gcaaatcagg caacggaccc gtgcataagg tatcctgata
     5881 accagatagt cgacagtttt ttaaagatac gcgatctcct gaattgaata aagcaacagc
     5941 ctgtcctacc ttttctcctg gacctgctgt attttcgatg tgaagatttt ctaatacgac
     6001 attcgatcct tctaagaaaa aagtagcact tcggaaagtc cctaactggc taccgtcaag
     6061 atactcctgt tttgcaaaac gattaccgga gataactact gttcctgctc ccacaagttt
     6121 aaaattggat aatctgcttt ctatatattc atcatatctt ccttctttta ctatcaagac
     6181 ctttggatgg ctatccggca ggcctgccaa ataatcgatt ccttcttgga tagtttcaaa
     6241 atccgtcggg gtatctatag cattctttcc aattatcagt ttgtctttca tcctcataaa
     6301 tgctccctta tgaattgttt ccaaatattt ctcatttctt gtgtttccat atgaccgcct
     6361 gttaaagaaa aagaagaaaa gttttctgga ctacccattg attgatactt tttgtccagc
     6421 ccttttctca gcttcagtac gccttcgtcc aagcacatcg tatcgttttt tccaactaag
     6481 ctaagacgag ggcgcggagc gattttttct tggattgcta acgttgtata agctgtcaat
     6541 agatttggca catagtaata aaacccatga tgatctaggc ggcgatgtag caaaagtatt
     6601 tcgatgtcca cctgtgccgc tagatcaata caaactttga cacgagaatc caatgctgtc
     6661 acccaccagc ttaataagcc tcccatagaa agaccaatcg ttgcgagccg ttgagtatct
     6721 gtgtcttccc ttgtctccaa ataatcgatc aagctgatca catcataaat acgcattccc
     6781 cataacgttt ttcctgttaa taaaaattgt ttgaacaact cgctttcaga aatacctccg
     6841 cgttcttcaa atccccaagc atcaatcgac caaacagcat atcccattcc tgtcagtact
     6901 tctgcaaaag atggagaagc taagtaagga gcaccatgga tcaattcact tttcccgact
     6961 gaaaaatctc cgccgtgaga atgcaggtaa aggaccggcg gaaacggtcc ttctttttct
     7021 ggataagcat agagggctgg tactgtttcc aattcattta agtacaattg atagtattca
     7081 agcataaaac cgttttgttt ttctcggtat aatcgttttc cactaggggt atgtctgcta
     7141 gatggagcac ctaaaaaatc attcaactgc ataagcaaag tttttacgaa caaccagttc
     7201 atccatattt tctgattgat ttgattggaa tttctcaata ctgatttctt tagtattttc
     7261 taatagaaat gctggtccct cgtgattttc aatcgacatc tggttaaagg aaaccttacc
     7321 cgcaaagcca atataaaatc cttgtctgct catttcctct attcctgtca tcatagcagg
     7381 aacaccggga actgcatctt gggccatcga gacatcgata ttgtcaaatg tgatttcttg
     7441 tatgaattgt tccgctaatc cataaataaa acctgcagaa gcatggacat tccttgccgt
     7501 gatattcgaa aaatggatcc ttctgaatgc tggtgtccgt tcatctatcg gataagcctt
     7561 tttttcccat acatacggtt ctttccctcg cggaccacag aaataataaa gattcagtat
     7621 aaatggacac atcacattat ccatgactat attggatact cgaatatctt caacgatccc
     7681 gcctcgacct cgtctagatt tcaatcgtat tcctcgatca gtttcttgga agatacaatt
     7741 tgaaatggta atattacgaa tactaccact catttcactt cctaatacga caccgccatg
     7801 tccatggacc atcgtgcaat ttgtgatcgt gatattttcg caagcgatcc tttcgtacgt
     7861 atcttcagtc cctgccttga ttgcaataca gtcatctccc acgtcaatat ggcaattact
     7921 aatacgcaca tttttgcatg attctggatc aattccgtct gtatttggtg aatccgctgg
     7981 gttcaagatc gtcaagttat caaacgttgc attactgcaa agaatcgggt tgatcgtcca
     8041 acttggtgac tggatcaatt taatatcttt cacagtaatt cgatgacagt tatggaaact
     8101 catcaattta ggacgaggat aagcgagatt atctggttca tttctaaaag tatgccacca
     8161 tttttttcca tttccatcta atgtaccgaa accagtgaca gagatgttct ctacattttg
     8221 agcatagata caggaagcat aaacttttcg atggactcct tcccaacgtg aatgaacaac
     8281 aggataatct tttggatcgt cagagaattt cagaaccgct cctgcgctta aatgaagttc
     8341 gacattgctt tttaaaaata aagctcctgt tagaaattca cctgctggaa caactacggt
     8401 cccgcctccg tcacttgctg cagcatcaat ggcttgttgt attgcttcag tattcaattc
     8461 atcaattgat gcgccaaatg tcaaaatatc atacatattt ataatgcctc cacttcaatc
     8521 aatgcttgca agaatgcacc aacgcctttt tgatcattac aaataattgg ttcactgatg
     8581 tagtaagcat atgaaccatc tcgctgatct gctccgccta accctgctac ttgacagttt
     8641 ttattcaagt tcagccagcc ttcttttgtt tccagaacaa attcatcaag taatccttga
     8701 tgtgatttct tcacttgtgg aagccattct tctttgttca gcagtcccaa acgtattcct
     8761 ttagccattg cgtaaacaaa catactactg ccggaagctt ccagataatt tcctttccgt
     8821 tttgtttctg ttgttacttg ataccatgtc ccgctgtttt cttcttgata tggaagtaat
     8881 gcagccagtg tcttttctag aatttctttc agcaaagaac aatctgtttc ttcatgaagt
     8941 aattcatagg tatctgctgc agccatcaaa taccagccaa tcgatcggct ccaaaaatga
     9001 tgggatagac ctgtttttgg atcagcccat ggttggacct tcttttcatc ccatgcatga
     9061 atcagcaaat ttgtttttgg atcgaataga tgtttataac tgatttcaaa ctgtctcaaa
     9121 acatcttggt aatctttttc ttcactaaaa ttctggataa attcagcata aaaaggagaa
     9181 cccatatata agccatccaa ccaaatttga taaggataaa tttctttatg ccaaaaagct
     9241 ccttcggatg tgcgtggatg gtttgccaac tgctgggaaa ggagttctgc tgctttttta
     9301 tatttttctt cgcctgtttc tttgtacaat aaaaagaaaa gctttcctgt atttacatgg
     9361 tcgatattgt attcttcaac gctgtagcca cggatcgttc catcttcttg cacgaaatag
     9421 tcgatattgt cttttatgaa atcaaaataa attttttctt gggttcgttc ccaaagtaat
     9481 tggaaccctt taagtacgac gccatagtca taagaccatt ttcctttata ccacttttct
     9541 tcatataagc gaggtgttct ttccatgata ctttttgcta atttctttga ccagttttcc
     9601 attatttgct ctcctttaaa aattaaaata ggatttggga caaataccaa gaagtatttg
     9661 tcccaatcgt ctatattact gtgccgctgc tttgtatgct tcctcgtact cttgtgtgat
     9721 tcgatcgcct ccttgtgctt tccagtcttc tacagctttt ttgaatccat cttcgtcaag
     9781 ctgtcccatg atgtattgca ccgtagcatc aatgacgatt ttttcaattt ctgatcctac
     9841 ttcattgttc gtcggtgaat ccaatggcac agaaggatcc attacagcaa attcttcatt
     9901 ttctttgatc aattgattag agaattcttt ttcagggtct gtatctttga aagagaacgt
     9961 cacttcacta ggacgagagc ttgataacgg ttgcacctct tgttgccaaa gatccatatc
    10021 gataaattcg atagaaccat catcattcaa ttcataatgc gttccttcga taccgccagt
    10081 catcaattta tacacatctt cttctaaaag atcatcaaca aattgaagta atttcttcaa
    10141 ttgtgcttcg tcttttactt gagatttggg aaatgctagc aagccgccaa taccatttcc
    10201 ttctgaccaa acatgataac cgccgccttc accatctgag atcttgttta caggcactaa
    10261 ttccatatca tcttgaatac cttgacccat cgtcttcaga ttcttgatat ccaccattcc
    10321 agtgtagatt ccagttcttc cttgtgcaaa ttgttcttgc tgatcagtct tttgggtaac
    10381 tgcaaagtct tgtgccaaat agccattctc aaatagatct cttgaaaaat tcaaagcttt
    10441 catatactct tctgtatcaa attctggagt gaatttgcca ttatcgtcta cagcccagcc
    10501 atttggtgct ccaaaataag aagtgaaaag tttaaagctg gtataacgaa tatcactagg
    10561 cgcacgatcg ccaaaaccaa ctgtgtcgtc cacaccattt ccatcaggat cgttttctgt
    10621 aaattgttta gctacttcat aaagctcatc catggtagtt ggaacatcta gtcctagatt
    10681 atccaaccag tcttttcgaa tgactaagcc agcacgagcg tagtcttttt gtatcggtac
    10741 gccgtacaag gcaccgtcga tactcgcagc ttcgattcta tcttcagaaa tttgacttaa
    10801 atttttatag tcttttaaat attctgatac atcccagaat acgcctgatt ttaacgcatt
    10861 gcggacagaa gagtttttca acatcgcaat ttgaagtgta acgatatctc ctagttcatc
    10921 agaagccaat gcagttgtta atctttcttc ttttgaagca tcaggaatcc agttgaatgt
    10981 cagttccgta tcggtttttt cttcgatcaa gttcacaatc gtgtctgtag gcggtgaagc
    11041 atgatgcaaa atatttaacc aagtgatttt agcttttcca tcttctgtct ctgaagaact
    11101 gttacttgaa ctgctgccgc atgcggcaag tgctagaatt cctgcaaaac taagacctaa
    11161 ttttacgagc gttgcttttt tcataaattt tctccttcac tgttttaata atatttttgt
    11221 tttcttctgt tttttctgta tatgagttct tccattccaa atggcaaaat atagaatttg
    11281 ctgtccacgt gactgcggaa aagaagagac tgccaccgat aaaggttaaa acacctggca
    11341 ttgtttggat gatcatcaga taaatcccta ccatacttaa tatcaacact aatgagtaat
    11401 gaagatgtga gaatccgaat aaaaatgcca ttttcatttt gaaaaaatat cctttccaat
    11461 cataatgagc catgattggg aaaatataga gtgctgagag cacatatatg aacgcagcaa
    11521 tatacaaagc gatcctaaga taccaattgt gtatgaaaga aagatccaca agtaaaacaa
    11581 tcccgatggc gccatatatc cagctcatca gcaaggtttc acgatagttt tctttaaaat
    11641 atgtgaagta cgttttcgtc aaaggaaacg tttcttttga gcgcagccat tttctaatca
    11701 ctgcaaccat cgcatacgat gcaggaccga aagtaaaaac tcccaaacct gcgatgacta
    11761 aaccgcccca cagaaaattc aaataaacca actgtaaaat acgatttagc cttccgttta
    11821 ttttatctaa tgactgcagc atatctttcc ctctctttct atcttagtaa acgccgtctt
    11881 ctcctagctt tttggcaatc ttattagcta aaattaccat aaacaatcct acgacccctt
    11941 taaacaatcc cattgctgta ctgaaactta actgtccatt cttcaatcca gctgtataaa
    12001 tataggtgtc gaaaatttct gcaacgcttc gattcaatga attcaataac aagtacatat
    12061 gctcgaaccc taagtctaat gtctgaccga tttttagaat cagcaaggta atgattactg
    12121 gacgaattgc cggcaatgta acatgccatg ttttgcgtaa gcgtcctgct ccatccattt
    12181 ctgcagcttc atacaactgt gtatcgactg cagttatcgc tgaaaggtag atgatcgtag
    12241 accagcccaa ctctttccag atcacttgtc cgatatatac tgtgcgtgtc cattcagggg
    12301 atgttagaaa actgattttt tcaaatccca ttgttgcaag gaattcattc aatacccctc
    12361 cgtcaacatt caaaaataca taagtaatcg aaacaatgat tacccaagac atgaagtgag
    12421 ggatgtaaat gatcgtttga atcccgtttt tcaatcgttt gttcgttact tcatttaata
    12481 gtaaagacaa aatgattggc atcggaaaaa acacgaagat atttaatccg aacaagatca
    12541 acgtatttct taataatgta aagaatgtcg gttctgtaaa caaacgaatg aaatgtttga
    12601 aaccgaccca aggacttccc attattccta aaaatggttg ataatcttga aaagcaataa
    12661 cgagtccccc cataggaaga tacctaaaaa tcaagaaata aaggactccc ggcagaatca
    12721 tcacatataa aaatttattc gttttgattc ctgcaattat tttttttcgt cttctgagct
    12781 tttcttcttt tttcgatagc gtcagttgaa atggttcttg tttctttaca ctttccataa
    12841 gcccctcctt agttttgtga atgtttgctg cttatgactt catgatagat aactatctga
    12901 attacgtata taatcatttt taccgtaagt aaaaacgctt acatagcaat gatttttgtg
    12961 ctatacaaaa cgataaagtg tattttttca caaataaact acgcatagca aaccttctat
    13021 ttttttctat ctgacactta gcataggaaa tgattatata cgtaatcttt caccgatgat
    13081 aaattgaaca tatagaaaca gaaacattta atttattttg aggaggagcc tatctatatg
    13141 aaaaagaaag tctctatagg agaaagaagt tttacacttt tcaacaatac gtttctaatt
    13201 ttgctagctt tgatctgtat cgttcctttt ttaaatatca tcgctacatc ttttgcttct
    13261 acacaagaag tcgtcgcgaa aaagtttatc ctttttccta ctaccttttc tttagacgcg
    13321 tatcgctaca ttctttctac gccgaccatt tttagagcac ttgctgtttc gattggtgtt
    13381 acaggtgtcg gaacgatcgt cagtatgtgt gcaacttcac tgatggccta cggtctttcc
    13441 agaaagtatt tgtttggtcg aggtttcgtc aactttcttg ttgtattttc tatgttgttc
    13501 agcgggggga tgattccaac gttcttagtt gttcgttctc tagggctggt caattcttac
    13561 tgggccatga tcctgcctgt ggctgttaat gcaatgaata tgatcattat gagaaacttt
    13621 ttccaagcct tgccggatag tttggaagaa tcagcaaaaa tggatggttg cacagatttt
    13681 ggtgtatttt tcaaaattat gctgccatta gcgttaccgt ctattgctac tatctcgctt
    13741 ttttatgctg ttacttactg gaacacttat atgactgcta ttttgtatat caacgattca
    13801 tctaaatggc cgatacagat tcttttacgc caaatcgtca ttgtatcaag tgggatgcaa
    13861 gcagaaagta gtgccgttga tgtgattcca ccagcacaaa cagttaaaat ggctgttatc
    13921 gttattgcaa cagtgcctat gctgattgtt tatccttttg ttcagaaata ttttgtcaaa
    13981 ggagcactcg taggttctgt caaagggtga ttatgtactg aacgcaaaat ggtagttgtg
    14041 tcagaagtgg tcagcttcaa gaaataagaa ggcatttacg aaaattgctt cttaaatttt
    14101 tgtgaaattt cggcttattt ttcgaagaat ctactcctgg aacaccgtgt atcagaggtt
    14161 gtgaaagaga cgttcagcct tgagcgatga taaaaactct cacaaacatt gcgacgagta
    14221 acccacagga gcaataagaa gcgattttga aaaatagctt cccatatttc acaaaattat
    14281 gccttattgt cgatagcctg tctcttgaac gccatttatt cgtaaaaaag aactgtgaca
    14341 agacttttgt cacagttctt tttgcgtgct tagccttgaa atttaactat ccataaatac
    14401 aaatactagg gttattctaa cttcttttat tgcttgaatc agtccacaag aaaaacagcc
    14461 tcttgattca caaatatttt tttcgatatt ctcccggcgt aatcgattcc ttctttttaa
    14521 agaaacgaat aaaattttgc ggatttcgat attgcaatct ttctgcaatc tcttttacgg
    14581 agaggttagt ctctttcaac catttttttg ctacctctaa acgatgattt tgaacaaaat
    14641 caccaaattt ttctcctgtt tcctttttaa atacattact taaatagtta gggttataat
    14701 gcaacctatc agcgatcaca tctaaagaca gatcacgatc atattctgtg tagacgatat
    14761 gcataatatt ctccgacaaa tttttaaatt catgatcagt gattgcttga gagcattctg
    14821 tgattggcag aactaattgt tctaaaacaa gtttttctag ttctttggga tgataattgt
    14881 tcagtgcttt tacataaatt tgtttcatgt tatcaaagtt ttttgaatct actcccaata
    14941 actgtcctaa ttgaacgagt tcattgatca agcggaccga aactacttcc cgactcaaag
    15001 gatttttatt ttgtgcaaac aatgcatcaa tcaattcatg tactaaattc ttcacttcct
    15061 gatttccaga acgaatggct tcaaacaatc gattttgttc ttctatcgga tatctaataa
    15121 gtgttttttc atgagcagca ggaacgattt cctgataaaa aataattgaa cttggaccag
    15181 tatttacacg ataatacagg gcttcttttg cccgatcaac cgattgcttg ctttcattca
    15241 gcgtttcgaa ccggttactg atgcctatac tgatagttaa attcaagtat tcttttacac
    15301 gtttttgaat gagtcggcaa tactccatga cttttttgct tgcttctgca tcatctttat
    15361 ccattcgata aatagtggct tgcatttctt cattcaaaac gatcggcagc attcgatttt
    15421 cctttggcac aatttccgtg atcatgttgt tgatcgctaa aagtagcaaa tcacgctccc
    15481 caccgtgact atcttctaac agatcgattt gaaccaaagc tgtataatag cagtcatttt
    15541 gagaactata accaaattgc tgcaaccgct gatctagttc tcgcctttct actcgatttc
    15601 taaataaact aagtacaaat agcgtttcca gctgtggctt ttgggtaact aagtgtgcac
    15661 ttaacgcttc tttttctcct atgatccgac tgactgattg agcgaccaaa tctagttctt
    15721 tcccactgaa tccttttgat ttaagattca gacgttcctg tatttgagaa atcggacggg
    15781 caaatctgtc cgaaaataag taggaaagaa gaccaatgag tcctaccagt gttaaagaca
    15841 ctgcaactag cccgattctc agattgcgga tcattgcgcc aattgcacta gagtcaactt
    15901 ctaatctgta aatccaatta ttataatctg atttcagtac tatatacgtt ttatcttttt
    15961 ctcttatcac tttcagctga ttgtaatcta gatccacgtc cgttaataca gttggttgct
    16021 ctttattttt tactgtctga gtagtaaaaa ggttgccaac ttcgttttca atagaaagta
    16081 tgtcttctcc actttcacca acaacttgtt ctattgtatg tttctctatc gtagcaattc
    16141 ccaatgcata tttttctttt tcaaacattg gcaaagaaat caccatttcg attcctttgt
    16201 ttttcttttg ccaaaataaa ttatttttgt tatttatgta cttctgttga tacttttcaa
    16261 cttcctcttc ggtcagttga ctcaacgatc cattgattac tccccattta ccagccaaac
    16321 tgactaaatc ataattatta tcatccataa caatcgtttc tataaaattt agttctttcc
    16381 tgatttcaga ataagtggag taatctgtat atgtcagcgg atgcttgaat atgtccgaga
    16441 aatttttggt cgaaccatac gtagaaaacg aatattcaat cgttctaatt ttccattcaa
    16501 cgttagtttt aacttgcctg agcaaattct ttttgtctgt taaataactc gattcgatcg
    16561 attcataggt attctggtaa atgaataaac taaatcctat gacaagtacg gctcctaata
    16621 aaaacatcgg taaaaatatt ttataaaata ctgtactttt cttcattttc ttcctctcct
    16681 ccttttaaaa taaaaggctt tcatttttct gtaaaattga tactcatcta attaaatgag
    16741 cattccccgc tttcaagaat agcataatta aaaataaaaa tccaagtaat ttttaacgtg
    16801 tgcaatcttt tcatatcaaa gatctattga cttatcgtag ttatcgtaat atgatgaatg
    16861 cgttaacaaa aagtgaaaaa gtaaggaaaa tgatggaatg gtaacgatca aagatatcgc
    16921 taaagcagct ggcgtttctc atacgacagt ttctcgtgct ttgaataaca gcagtttaat
    16981 aaaaaatgaa acaaaacaaa aaatacgaca actagctaag gaaatgaatt attcgccaaa
    17041 ctatagtgca aaaggtttag tgaatcaaaa aaaatatcta atcggactat ttttctcaag
    17101 tttgcataaa ggaacttcat caaatttcct agcagattcc attgcaggca tccattcagt
    17161 attagacaca aattacagtt tatctgttga aagtatcgat gttgttgatt taacagaaat
    17221 caacctgcaa cgctacgatg gactgatcgt tatgagtcaa agtgatgatg atcagccttt
    17281 catcgactat ctcaaagaaa aagaatttcc tttcgttgtt gtcaatcgtc acatcaatga
    17341 tccagcggtt atcaacgtca ctgccgatga tgcggccggt gttatgcagg cagtagatta
    17401 cggtattgga tgcggacata cgaagatcgc ttatattggc ggaaagaaaa attttcgttc
    17461 gacaaaagaa cgggaaaaag gggtattgaa aagtctaaaa aaagcgagca tccctgtcaa
    17521 tccagcctat tttttaacag gagattacag cttagaaagc ggcctccaaa acatggaata
    17581 cctattatca ctttctgacc tgcctactct ggttttttgc ggcaacgacg acatagcgat
    17641 tggtgcattg agagctgtac atttagctgg cctccgtgtt ccccagaaca tttctctgat
    17701 cgggttcgat gattctccag tcgtctccta tctcaatccg ccgcttacca ccgtacgtaa
    17761 gcctttgaaa gaaatgtgtc aagaaggaac aaagctttta ctagatttga tcgaaggcag
    17821 gacgatcgaa caatctaaaa ttgagctctc caccttgttg atccttcgtg agtcgatcaa
    17881 ttacttgtaa actacaagta attttatata aaaatgttaa cgtgtgcata acacagaaag
    17941 gaagtttata tgaaaaaact gacaaaagaa ttagtgaaaa aaaacgaagc acctgtaaaa
    18001 gttcttcagt ttggtgaagg caattttatg cgtggattta ttgattggca gattcagcag
    18061 ctgaataacc aacaattgtt taaaggtaat gttgcaatca ttcaaccttt agaacaaggt
    18121 cttggaaata tgatgaacca gcaagataat ttatatacgg tcattttaca aggactcagc
    18181 aatggcgaaa tcgttgatac ttccgagatt atcaccgtag tagaacaaat gatcaatcct
    18241 tatgatgaat gggatgctta tctatctcta gcagaaaatg acgacttggc ctatatcgtc
    18301 tccaatacaa cagaggcagg aatccaattc gtggagactg ataatttaac agatactccc
    18361 ccacagagct tcccgggcaa actgacagcc tttttatacc gacgtttcaa attaaacaaa
    18421 gctggcttta ccatcattcc ttgcgaactg attgacagaa acggcgaacg tctaaaagaa
    18481 atcattttac gctacgcaga aaaatgggat ttagaacctg cttttgttga gtggttagaa
    18541 aaagaaaatg ttttctgttg tagtttagtt gatcgcatcg tcccaggtta tcctcgcgaa
    18601 gatgcaggtg ttttcaatca acggcataac tatgaagatc aattgatggt caaagcagaa
    18661 ccattcatgc tttgggtgat cgaagggcct aaagaattgg agaatcaact accattcaaa
    18721 caggctggct tgaatgtcat catcacagat gatatgactc cttatcgtga acgtaaggtg
    18781 catttgctta atggaccgca tacagcgatg gtccctcttg ctttgttggc ggaattggaa
    18841 actgtagaag aagtaatgaa agatccttta ttctccgcat atgttgatca gttattcaat
    18901 ctagaattga ttcctatgct ttctttacct aaagatgaat tagccattta tgctgaccaa
    18961 atcaaagaac gtttcttgaa tccttttgcc catcacaaat tagaagcgat ttcattaaat
    19021 agtgtttcaa agttcagcac ccgtctgctt ccagtgttca agaagtatat cgaagaacaa
    19081 aatcaggtac caccgttaat cactgtttca ctagctgcgc tacttcttat gtatcgaggc
    19141 gatcaagtaa agccacatga tgatgaaaag acgataagcg aatttactga tgcttggtct
    19201 gataatggaa cagctattcc tcgattattg caaaacgctg cgctttgggg agaagatttg
    19261 tctcaaatac cgaatgtgac agatacagtt caagaaatag ctgcaaagat cgatcaaaaa
    19321 ggtgtccggt cagtattaca agaaatgaaa ggaacgatgg tccatgcttg aaaccctgca
    19381 accaacaatg aaattaaatc gacgagacag tgtggtcgtc gcattaaccc ctattccaaa
    19441 acaaacacaa ctgacgattg atcagcaaac gatcaccact cttgaagata tcccgcaagg
    19501 tcataagatt gcattgggtg atttgaaagc tggagacaac gtgatcaaat atggctatcc
    19561 aatcggtcat gtgacgacag acgtaactgc cggtcaatgg ctgcacactc ataatgtaaa
    19621 aaccaatctt tctggtgaat tggattatcg atatgaaaaa gatgtccatc caagtcatta
    19681 cctttttgag aaccgaacat ttcaaggtta cttgcgaaaa aatggaaaag tagggattcg
    19741 taatgatttg tttttagtac ctaccgttgg gtgtgtgaat ggcattgctg aactgatcgt
    19801 caaacagttc aaggaaaaac atccagatct gggacagttt gaccatatta cgattttaaa
    19861 gcatccttat ggctgttcgc agcttggaaa agatcatcaa aatactcgag aaattttagc
    19921 ggacgcagta aatcatccca atgccggagg agttttagta tttggattag gttgtgagaa
    19981 caatacagtt cctgaattca aaaaaatttt aggtgaatat gatgaagaac gagtgaaatt
    20041 tctagtggct caagatgttt acgatgagat cgaacaaggt gtggctctat tagaagagct
    20101 tttaacagcg gcggaaaatg accagcgcat atcagtcccg ctctctaagt tgaatgtcgg
    20161 tttgaaatgc ggcggttctg atggtctatc gggaataact gctaatcctt tacttggtgc
    20221 attttctgac tacttgattg ctcaaggagg cagcacgatc ttaacagaag tgccagaaat
    20281 gtttggcgca gaacaagtat tgatggcacg agcggaaaat gaagaagttt ttgactcaat
    20341 cgttgacttg atcaatgact tcaaacagta tttcttgtct tacggggaac cagtttacga
    20401 aaacccatct cctggtaata aagcaggtgg gatcactaca ttagaagata agtctttagg
    20461 ctgcacgcaa aaatcaggaa gctctcctgt cgttgatgtg ctacaatatg gcgaaaaaat
    20521 cagaaaacca gggcttagtt tattgcaagc tcccgggaat gatcttgtag ctgcctctgc
    20581 tcttgcttca tccgattgcc agttggtctt gtttaccaca ggaagaggga ctccgtttgg
    20641 ctcatatgtc ccaacggtca aggtatctac gaatacaact ctttttgaac gcaaaggtca
    20701 ttggatggat tttaatgcag gcgtcttatt agaacaacca atggaagtaa tcttagaatc
    20761 gtttgttcaa aaaatcatcg ctgtagctag tggagaagaa acgaaaaatg aacaaaatga
    20821 cgttcgagaa attgccattt ttaaaaacgg agtaacactt tgattttcat aaaacagtat
    20881 aaaaagaaag aaggaagatt tatgttttta tctgatgatt ttttattaaa agacgattgg
    20941 gcgaaaaagc tttaccattc ctacgcaaaa aacatgccca ttattgatta tcactgtcac
    21001 ttagatccaa aagaaattta cgaaaacaac aatttcagca atctgacaga agcttggtta
    21061 ggaggcgatc attacaaatg gcgcttgatg cgagcttgcg gcgtgcctga agaaaaaatc
    21121 acaggaaatg cttctgattt tgaaaaattt ttagcttggt gtcaaaccgt accaaaaatt
    21181 atcggaaatc cgctttacag ttggtcacat cttgaattga agcgtttctt cggtattgac
    21241 ttgttgataa atgaagaaaa tgcagagaaa atttggacac aagcaaatga aattttggca
    21301 gctcctgatt ttcgccgtcg ggaaatcgca aaaaaatcga atgtagaagt gatttgtaca
    21361 acagatgatc ccgcagacga cttgcactat catcagttgc ttgctcatga agaaccagat
    21421 ttgaaggttc taccttcatt ccgtccagat aaagcactca atattgaaaa agaaggattt
    21481 aatgcatggg tcacactact ggaagaagca gctgaccaaa cgatcaccac ctatcaagaa
    21541 ctgatagaag tcttagggca gcggatagaa tacttccatc aacatggatg tcgtgtttcc
    21601 gaccacgctt tagacatctt acgctataaa gaagcggatg aaacagtatt agaagagatt
    21661 tttcaaaaag cacgagctaa caaactatta tcttcagatg aaatcgatgc ttatcgtaca
    21721 gaaacactga tacatttgac ccacttctat cattctcata attggacgat gcagctgcac
    21781 atccatgctt atcgtaactg taatacacaa atgttggaaa aattagggcc cgataccggc
    21841 tatgatggaa tgaatgatct ttctttgact attcctctgc aaaaattatt gaaccgtgca
    21901 gaagaaacgg atcagttgcc aaagacgatc ctttattcat tgaatccgaa tgactatcct
    21961 gcgattttag ctttgatggg ctgtttccaa aaagaacaga atggaaaact gcagttaggt
    22021 tctggatggt ggtacaacga tactcgagct ggtatgcgcg atcaattgac acaatttgct
    22081 gatggtagtg ctttggggaa ttttgtcggc atgctgacag attcaagaag tttcctgtcc
    22141 tacactagac atgaatattt ccgtcgtgtt ctttgtgaat ttttaggcga aatggtcatg
    22201 cgaggagaag cacctgaaga tgaacaactt ttaggaagtc tagttcaagc gattagttat
    22261 acaaatgcta aaaattactt tggctttttt gcctaattga ttaatagtaa cagtaacgaa
    22321 tcaaaaacag gatagtgaaa taacggaatc cgtttatcca cgttttagag aagaagacgg
    22381 accaaatttg ctccgtcttc ttctcttgct gttctgcttc attgcctata aacctttatg
    22441 aaagaagaga taacatgcct actatccata ttgccggtga ttctactgcg gcagaaaaag
    22501 atgaaaacaa gcgtcccgag acaggttggg gagaaaaaat ctctgcttat ttttcacccg
    22561 agatccagat caataaccaa gcaaagaatg gacgcagcac caaatcattt atccaggaag
    22621 gaaggttagc ccgtctagct tcacagtttc agccgaatga tttcttattt gtacaatttg
    22681 gacataacga ccagaaaatc caagaagaaa gaggtacgct tcccttcggt agctatttaa
    22741 aaaatctgca gatatttttt gaatgcgcaa aaaaagcgaa cgtccaaatg gtcttactga
    22801 catctgtgac gcgacgagat tacttagaaa acggaacatt gaatccagat atacttggag
    22861 actatccaaa agcgatgcgt gcatttgctg agaaacacca tattcctctt ttagatgtgt
    22921 tctcaagatc acaagaattg ttccagacct tctcaaaaga agagacaaaa aaattctacc
    22981 tgcatcttat acctgatact tgtaaaaatt acccagaagg actaaaagac aatacccact
    23041 ttagtccaga aggcgctgat aaagttgccc gaataattgt tgaattgata aaagaatccc
    23101 gtctgcctct tgctaattat ttacaaaaag gagtttgaga tgtttccatt aaatttaggc
    23161 atccgtgccc acgatttaga tgttcgaaat cgtgaagaaa tcgttcaaaa agtatcacag
    23221 cttcacttgt cccatatcca atttgcacca caaaaagcat ttcctgaaca acttaaatta
    23281 actgacatga cacttgggag tgctgcctat tttggtgaat acttccaaaa aaacaatatc
    23341 gaactttcca ttttaggatg ctatatcaac ctatccagca aaaatcaaga agttcgtgca
    23401 caagcattgg caactttcaa acagcaaatt gctctgtgct ctgcctatca agccaagatg
    23461 attgctacgg aaaccggtag tgtctctgtc ggttacacaa aagaaaattt tactgaagaa
    23521 gcttatcaga tcgccagaaa ttctatcatt gaattagtcg ctcatgcaga aaattttggc
    23581 gtaactgttg ctgttgaagc agggatcaat catccaatct ataactatca gttagctaaa
    23641 cgtttaatcg atgaagtagc ttcaccaaat ttaaaaatca ttttagattg tgcgaatttg
    23701 atgcataaag aaaatcatgc tgatcaggaa aaagtgatcc gaggtgcctt ggacaattta
    23761 catggttata ttacagcgct tcatctcaaa gactatatca tggaaaatgg aaagatccgc
    23821 atggtacctg ttggcaaggg ctgtttagat ttccgcccgg tactgcatta tatcaaatat
    23881 gagcgtcctc taatgtacgc cactcttgaa gctactcctg agatttatgt tcctgaagca
    23941 atagaacact tgcagtttat ttaccaagat tgttaaggag aagattatgg aggaacgaat
    24001 acagaaaagt tatcaaaaat atcatgcaaa tgcttttcag acattgctgc atctttatca
    24061 gggaaattat tggcgttttg ccatgtcagg actctttttt attatcaaac atcttccatc
    24121 ttggttaatg ccgatcgcta ttgccaatgt aatcaatgca gcttcatcga aagattcttc
    24181 tcagttgcag gccatttatt tcaacgcttt tttgatggct tgtctgattg gtcaaaatat
    24241 attgacgaat tattttcatg taaaatacca tagcacttcg atccgtcaag tagaagctgg
    24301 actaagagcg acccttatca agaaaatcca agaactatct attccttatc aaaaagaatt
    24361 gcaatctgga cgaatccagt caaagatcat ccgtgatgta gaagctatcc aaacgttgtc
    24421 ctctcaagta tttgtcagcg gattaaatat cgctgtcaat ttattcgtag ctttaggtat
    24481 cacgctttat aaaagtcctg ttgttttcat gtttttctta ttgactgttc ctctcgccgc
    24541 attcgttact ctctttttcc gtaaaaggat cagacagtcc aactcagact tcaggcaaga
    24601 aatggaagag acttctgcaa aagtgatcga agtgatcgaa ttagcaccga ttgctcgagc
    24661 tcatgggtta gaaaaacacg aaacaactcg cttaaataag cattttactc aaatctatga
    24721 aaaaggattc cacttagata tcctgcaatc tgttttcgga tcagtcagct gggctagttt
    24781 tcagatattt cagctgattt gtttgatttt cactgggata ttagctttac aagggcgcat
    24841 ccaacccggc gatgtcgtga tgtatcagac gtatttcacg acgatcgtca attccgtttc
    24901 tggaatgatc acgctgattc ctacgatttc aaaaggcatg gaatctatca cttctattgg
    24961 cgaactgttg attgctggcg atgtagaaac ttatcacggt aagaaaaaaa tccaacattt
    25021 ggatggaaat tttcgttttg aggatgtttc ttttctttat ccggactcac tcaaaccgat
    25081 tatcgatcat ttttctctag aggtgaaagc tggagaaaca attgcttttg ttgggccatc
    25141 tggctctgga aaatctacac tccttaactt attgattgga tttatccagc cgacttcagg
    25201 aaaaattttt ctagatgatc agcctttgga tgaactggat atgcgaagtg tgcgaaatta
    25261 tttagccgtt gttcctcaaa ccactttgtt gttctccgct tcgatcaaag agaacatcac
    25321 ttatggcttg aaaaacgtat caaaagaacg tttagatgaa gtcatcgaag ctgcgcaact
    25381 ttcttcactg attgaccagc ttcctgatgg tctggatacc ttagtaggcg aacatggcaa
    25441 caagctgtct ggtggccaaa agcaacgtat ctccattgca cgagcgttga tacgtcagcc
    25501 aaaaatcatt ttgctagatg aagcaacctc agctttagat aaccagtcag agaaaaaaat
    25561 ccagcaagcc ctgaattacc tgacagaaac acccactact tttattgtgg ctcatcggct
    25621 ttcaacgatc aaagaggcag ataaaatcgt tgtgatcgat gaaggaaaaa tcgttgaaat
    25681 aggtacttac gaaaaactgt tagctcaaaa aggctatttc taccgcttga aaaatgaatg
    25741 acaaaaaata
//
LOCUS       DACDIH010000034        25508 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDIH010000034 DACDIH010000000
VERSION     DACDIH010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.034"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..484
                     /locus_tag="IV281_001503"
     CDS             <1..484
                     /locus_tag="IV281_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8780499.1"
                     /translation="LADTAVWISQEEKQRYGSWIFIKKFFKLLLKKKKYHLDLETDEE
                     HWKGKSQLLTVTMSNSVGGFTNFDDSATLDDGKFHVTIVPSLDIFRFIFYLPRIMRGK
                     FYTIPGITYFTAKRMKIQAQEKSVGTRTDGDTTDDLPVELSVVSKGLTVYSPKKEEKA
                     "
     gene            519..791
                     /locus_tag="IV281_001504"
     CDS             519..791
                     /locus_tag="IV281_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP8780500.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            802..1848
                     /locus_tag="IV281_001505"
     CDS             802..1848
                     /locus_tag="IV281_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP8780501.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            2099..4708
                     /gene="clpB"
                     /locus_tag="IV281_001506"
     CDS             2099..4708
                     /gene="clpB"
                     /locus_tag="IV281_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP8780502.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(4859..5530)
                     /locus_tag="IV281_001507"
     CDS             complement(4859..5530)
                     /locus_tag="IV281_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780503.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(5523..6017)
                     /locus_tag="IV281_001508"
     CDS             complement(5523..6017)
                     /locus_tag="IV281_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP8780504.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(6037..7257)
                     /locus_tag="IV281_001509"
     CDS             complement(6037..7257)
                     /locus_tag="IV281_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8780505.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            7404..9239
                     /gene="lepA"
                     /locus_tag="IV281_001510"
     CDS             7404..9239
                     /gene="lepA"
                     /locus_tag="IV281_001510"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP8780506.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            9435..10346
                     /locus_tag="IV281_001511"
     CDS             9435..10346
                     /locus_tag="IV281_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8780507.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            11342..11593
                     /locus_tag="IV281_001512"
     CDS             11342..11593
                     /locus_tag="IV281_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780508.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            11676..11948
                     /locus_tag="IV281_001513"
     CDS             11676..11948
                     /locus_tag="IV281_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780509.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            12038..12277
                     /locus_tag="IV281_001514"
     CDS             12038..12277
                     /locus_tag="IV281_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780510.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            12424..16707
                     /locus_tag="IV281_001515"
     CDS             12424..16707
                     /locus_tag="IV281_001515"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAP8780511.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            16806..17171
                     /locus_tag="IV281_001516"
     CDS             16806..17171
                     /locus_tag="IV281_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780512.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            17173..17625
                     /locus_tag="IV281_001517"
     CDS             17173..17625
                     /locus_tag="IV281_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780513.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            18239..19549
                     /locus_tag="IV281_001518"
     CDS             18239..19549
                     /locus_tag="IV281_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP8780514.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            19572..19883
                     /locus_tag="IV281_001519"
     CDS             19572..19883
                     /locus_tag="IV281_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780515.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            20244..21500
                     /locus_tag="IV281_001520"
     CDS             20244..21500
                     /locus_tag="IV281_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8780516.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            21497..22042
                     /locus_tag="IV281_001521"
     CDS             21497..22042
                     /locus_tag="IV281_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780517.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            22043..22543
                     /locus_tag="IV281_001522"
     CDS             22043..22543
                     /locus_tag="IV281_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780518.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            22624..23180
                     /locus_tag="IV281_001523"
                     /pseudo
     CDS             22624..23180
                     /locus_tag="IV281_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23200..23544
                     /locus_tag="IV281_001524"
     CDS             23200..23544
                     /locus_tag="IV281_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780519.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            23563..24474
                     /locus_tag="IV281_001525"
     CDS             23563..24474
                     /locus_tag="IV281_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8780520.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            24553..>25508
                     /locus_tag="IV281_001526"
     CDS             24553..>25508
                     /locus_tag="IV281_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8780521.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLT"
BASE COUNT         8717 a         4073 c         5349 g         7369 t
ORIGIN      
        1 cttagcagat acagcagttt ggatcagtca agaagaaaaa cagcgttacg gctcgtggat
       61 ctttatcaag aagtttttta agttattgct gaagaagaaa aaatatcatt tggatttaga
      121 aacagacgag gagcattgga aaggaaaatc ccaactgttg acagtcacga tgtctaactc
      181 cgtgggcggt tttacaaatt ttgacgactc agcgacccta gatgatggaa aatttcatgt
      241 aacgatcgta ccttccttag atattttccg atttattttt tatttgccta gaatcatgag
      301 aggcaaattc tatacgatcc ccggcattac ttatttcaca gccaaacgca tgaaaatcca
      361 agcgcaggaa aaatctgtgg gcactcgaac ggatggagac acgacagatg atcttccagt
      421 tgaattatct gttgtatcaa aaggattaac agtttattca ccaaagaaag aagaaaaagc
      481 ttagaaatag tagttaacag aaagttggaa gaagagtaat ggatgtattg ttacacaatc
      541 ctttgtggtt ttaccttttc attgtgaatt tttacttatt ttgtctgatg gggtatgata
      601 aacatcaggc tagaactagc ggctggcgtg ttcctgagaa taatttgata tttgtcagcc
      661 tgttaggcgg tggtttggga ggattgatct ctcgaaaagt tttccatcat aaaactcgga
      721 aaaagaagtt tactatagga ttcgctgcag gtgtacttgt tgatttgatt ctcatttatt
      781 ttttccatta aggagcagga gatgtcgtct aaaggaacga aaactaaaat cattctgaat
      841 ctgcttttgc tgattgtgat ttttgccatt atttattatt taatcaatca atcatttgca
      901 gatatctttg ccgaattgct gtctaccagt gttgttgttt tggcagcggc tgttatatta
      961 gggacagttt accaaatcgc tgaagggcgc tcaatcaaag aaattgctga acctttcgcg
     1021 ccgaatttta caacgaaaga tgggttcttg acctctagct atattgcgtt ttaccgaatc
     1081 gtttcgtttg gtacaggaac gctgatctcg gagatttatt tttataacaa aaaaggaatg
     1141 aagctatccc agggagcagg ggtgacagca ctccatatga ttatgtacaa actggcagtg
     1201 atcacttatg cattagctgg cttggtcatc caattctctc ttttttattc taatggtcca
     1261 tcaatgatct ggtttatcat cgctggtatc cttttgacga tcgtgattgt tgccttttta
     1321 ttggcgatgt ccgtcagctt gaatgttcaa atattttttg tcagcttatc gaataagtta
     1381 tttaaaagtg agcgtttgcg gaaatgggtg gatacatgca atcagcagat ctactcttta
     1441 agggaaacgg tccaaaccat tatccaagac aggactgcgc tattgcggat ttatctatgg
     1501 aatatgttaa aacttgtatt ttggtatgta cttccttatt tgattctgat tgaaaaccat
     1561 cctaaaatag attttctact gaccatttcg tttatcagct ttgcagtagt gctttcaggg
     1621 gttattccta cgcctgctgg tattggttcc tttgaatttg tctatttgtt attattcaga
     1681 ccagttgttg gaacagttga cgcagtttct tcgcttttac tgtatcgttt cagttccttt
     1741 atcttgccat ttctctatgg atttgtgtat gtgattgctg aacggcgaag agtgatcaaa
     1801 gaagaaataa tagaagtaaa aaaagagaat aaagaaaatc aagggtaggt cttttataag
     1861 atctaccctt tttatcaaat caaagaaaaa cgttatggat aagataattt tacgtttgtt
     1921 tggctctttt tttatataca tacaatcaat tttgtttttt taagatttat ataagtcgag
     1981 aaaaaaagtc aaaaaactat gtgtatattt tgaccaaaat tgaccaaagc gatataatag
     2041 tttttgtaaa gatcaaagaa ataagtgatc ttacctttaa ttaggagggt atacctgtat
     2101 gaatattgag aaaatgacta cgacgcttca agaagcgatt gctgaagctc aacagattgc
     2161 ggtcactcga aaacatcaag atatcgacat tgcccatgta tggaaaatct ttttacaatc
     2221 taatcatttt ggtcgaaatt tctatacgga tgccggattg gatgtcgaat cttttgaaca
     2281 tgaaattgat cgtctactgg atgagtatcc agttgtttct ggtggaaacg tccaatatgg
     2341 acaaaacctc agccagaatt tctttcgatt attgaatgaa gcagaccaaa tacgtgaaag
     2401 cttcggcgac gagtttcttt caacggaagt cgttatttta gcgttgatga aattaaaaaa
     2461 ttatccgtta actgtttatt tgaataaaaa cggattatct gaaaaagaat tgcgtaaaaa
     2521 catcgaagag atgagaggag gagaaagagt gacctcacaa aaccaagaag aacaatacaa
     2581 agcattagaa aaatacggcg ttgatttagt acagcaagtc aaaagcggaa aaatggatcc
     2641 aatcatcggt cgtgatgaag aaatccgtga tgtcatccgt attctttcac gaaaaacaaa
     2701 aaataatcct gttctgatag gagaacccgg tgtagggaaa acggcaatcg ttgaagggct
     2761 tgctcaacgt atcgtacgca aagatgtgcc tgaaaattta aaagataaaa cgattttttc
     2821 attagatatg ggtgcattga tcgctggagc aaaattccgt ggtgaatttg aagaaagact
     2881 aaaagcagtc ttgaaagaag tcaaaaaaag tgatggacgt atcctcttgt tcatcgatga
     2941 aatccacaat atcgtagggg caggaaaaac agaaggaagt atggatgccg gaaatctgtt
     3001 gaaaccaatg cttgctcgcg gtgaattaca tttgatcggt gccacaactt tagatgagta
     3061 ccgtcaatat atggaaaaag acaaagcgtt ggaacgtcgc tttcaaaagg tattagtcaa
     3121 agaaccaaca gtggaagata cgatcagtat tctgcgtgga ttaaaagaac gttttgagat
     3181 ccaccatggt gttaacatcc atgacaacgc attagtagca gcagctactt tatctgaccg
     3241 ttatattact gatcggttct taccggacaa agcaatcgat ttgatcgatg aagccagtgc
     3301 aacgattcgg gtagaaatga actctatgcc aacggaatta gatcaagtta ctagacgttt
     3361 gatgcagttg gaaatcgaag aagctgcact gaaaaaagaa tcagatgatg caagtaaaaa
     3421 acgcttgaaa aatttgcaag aagaattagc tgaacttcgt gaagaagcaa atgcgatgaa
     3481 gatgcagtgg gaaacagaaa aagaagaagt caattctgtt tcagcaaaac gtgctgaaat
     3541 cgataaagca aaacacgaat tagaagatgc agaaaataac tatgatttag aacgcgctgc
     3601 tgtattgcgt catggtacga tacctcaatt agaaaaagaa ttgaaggaat tagaagctaa
     3661 agcaaaagac agcgagatta agatggttca agagtccgtt accgaaaatg agattgctca
     3721 agtcgttggc agattaacgg gtatccccgt aacgaaactt gtagaaggtg aacgagaaaa
     3781 actaattaaa ttgaatgaaa cacttcataa acgagtgatt ggtcaagatg aagcagttga
     3841 tgcggtcagt gacgcggtaa ttcgttcacg ggctggattg caagacccaa atcgtcctct
     3901 aggttctttc cttttcctag gaccaactgg tgtcggaaaa accgaattag cgaaagcgtt
     3961 agctgagaac ttgtttgact cagaagatca tatggtacgg atcgatatga gtgaatatat
     4021 ggaaaaacat gctgtgtctc gtctagtcgg agctcctcca ggctatgtag gttacgaaga
     4081 aggtggtcaa ttaacggaag ctgtcagacg aaatccttat acaatcgtct tgttggatga
     4141 aatcgaaaaa gcgcatcccg atgtcttcaa tatcttgtta caagtcctag atgacggacg
     4201 cttgactgat tcaaaagggc gagtagtaga tttcaaaaat acggttctga tcatgaccag
     4261 caatatcggt tcacagttac tgctggaagg cgtgactgct gatggaacga ttccagaagc
     4321 agtggctgaa caagtcaaca cgctattacg tggaaacttc aaaccagaat tcttgaaccg
     4381 aatcgacgac acgattttat ttacaccatt gagcctagac aacgtgaaag gaatcgttga
     4441 caaaatggtg gcgcaattgg cacaacgtct ggaacatcaa gaaatcctgc tgacaatcag
     4501 tgatgaagcc aaaacgtgga ttgctgaaaa tgcttatgaa ccagcatatg gtgcaagacc
     4561 attgaaacga tttatcacta aggaagtaga aacaccacta gctaaagaaa tcgttgcagg
     4621 acatgttatg cctaaatcaa aagtaacgat tactttactt gatggacaac ttcatttcaa
     4681 aacagaagag ctagaagaaa tcgtctaatc ttccattgag actataaaag aaaaaagggg
     4741 tctgacacaa ctagtgtcag acctcctttt ttcaaataaa cagtgttcca gaagtagttt
     4801 cttcgaaaaa taagccgaaa tttcttgaag ctgcccgctt ctgtcacaac ctcttttatt
     4861 aggtattttt cactttttct aatttataac tttcaaagta acgctcatct tttttacttt
     4921 gagaccatgc aaacaatccc attaaggcag cacctatcgt atttgtcagc aagtcgccca
     4981 tcgtatccat caatgctgct cgtccgacaa acagtgtgcc atcagaagca gcaaaacgtt
     5041 gaagattcat gcctaagaac tggtcgcata agaattccca gaactcccaa aacacgccgc
     5101 aaagtccagc aaacgcaaag ccgaataaga ggaacaacca aggactggtt tttgatatct
     5161 cagcatcttt catcaagtaa ccaatcaatc catagccaag agctgtcagg accattggac
     5221 tcaccgcatg taagattttg tcccaaaaag agatgatact gatcaaatgc atccctgtgc
     5281 ctaaaaagac agagatgaac aagaaaaacc agtaaaacaa aacgattgct gctggtattt
     5341 taagtttgaa aactttcaaa atgacttgtg gcatgaaaat caatgcaatc cctaataaag
     5401 attccacgat aaatgtgatt ccttgagaag tcgaatattt ttgtgtgata aattcataaa
     5461 aattatagca gacaacgaat acaccaaaag caagtaaagc tatttgcacc tttttgattt
     5521 ctttaatcac tgaaagtccc tccatctaaa aatcttttta cttctgaaag caactcaatt
     5581 tctgaagaaa ccacttttcc gttcaatttg atcaaaccta ttgtatataa gttcaggtag
     5641 tgaaattgat tttcagctgt ttcttgtagt gcagcgattt ttttcgggtg ggttccgcct
     5701 tgctgtcgag tatcagtata taagccgatc actgggatgc ctttcgcata agccacaccg
     5761 atttccgatg ctaccccggc atcgatcgtc agcccatcta gtaaagcaat cattaaatca
     5821 ctagccaaca ctttttctgt gtcagcaaga gcaatcattc gactgtctgc ataagcagtt
     5881 ttgtcattga ttgcttcatt ttcttgagga agatagatcg aaagctcaga agagagctct
     5941 ctgatttctt taactaactg ttggttatat aacaaatcac ttttagcaaa cattggtgcg
     6001 gcaaaataaa ttttcatcta aacatcatcc tttttatcaa aaataatcgc taaagtaagc
     6061 ttccatgttt ggaatgatac gctctagtga cttcagtgtt tcttcatctg tttctaaccg
     6121 ttcgattctt gccaaataag aagcacggcg ataattcatc atcaagcaag tcaatgtttg
     6181 gatatccagt cgaacggatt ttcctatagg ttcatttaag acacggactt gtccctgttc
     6241 atcccatgtt aaagcgaaga caccattatt ccaaggagcg acaggatctt caatgatgaa
     6301 atgaaaagca tcagctgttc caacaaacgg aaaccgttga aggaattctt ttacatcaac
     6361 gatccgagcc ataaaatatg gttcgatctg ctctttgatc tgactatctt caatcaaaaa
     6421 tgctaaaggc tcatttttat aaatatctcc atgtacccag tagatcatgg aaaaatgtgc
     6481 ggaaataaag ttccataagc catttcgagc ttcttgatca agatagaaca tttcttttac
     6541 gtgaaatatt tcttcagcta cccagtagaa cagaacacct cttggttcat ggttgctatc
     6601 gtagtagaca gcggctgtgc gctcatcttc attttcgaaa cgccaatact cttcccaatg
     6661 aaaatcactg cggaataaag caccatggtt ttgttgggca aatttgtcat aaacggaaaa
     6721 tacgtctgga tgatcaactg gtaatcgctc aaccatacca cttactggga tttgtttagg
     6781 tagctgtgta tctctgattt taaaggaaag tttatcagac atgatttccc atcctttacg
     6841 ccgatagtag gggatactgt aaggatacaa ataagagatc cattgcttat tcttgcgcat
     6901 attatccaat gcaacgataa ttagatcttg catcaatcca tggttagcat attctggata
     6961 agttccaact ccagtcacac cacccatttt ataacgtgca ccgtgaatat tgacttcaca
     7021 aggatagata gcgatttgag agatcagttt attttcgtgg aaccatccga acactttcga
     7081 tacttccaaa atcggttttt tggaacggat aaaagctcgt ttattctcaa acccgctttc
     7141 ctcgatgtct gcttcagtca cttggaacac ataggaaagc aattcattga attgatcgat
     7201 atgttcttga gtgaccggtt ttaattcaag attttctcta aaatcttttt gatccatttt
     7261 ttcatctcca tttattagct tgataacagt atagcaaaat tctacaaaaa aaacttctat
     7321 agctgactaa ttaaaaactc tttaaattac tgcttttcgt tggtataatg gaaagtgctg
     7381 attatatgaa aagaggagac gctatggata taaagaaaat gaaagaacgc caagagcaga
     7441 ttcgtaattt ttcgatcatc gctcatatcg accatgggaa atctacattg gccgatcgta
     7501 ttttagaaat gacgcacact gtgacgtctc gcgaaatgca agatcagctg ttagactcga
     7561 tggatttgga acgtgaacgt ggaatcacga ttaaattaaa tgctgtagaa ctcaattaca
     7621 cagcgaaaaa tggagaaaca tatatctttc acttgattga caccccaggg cacgtcgact
     7681 tcacctatga agtctctcga agccttgctg cctgtgaggg tgccgtttta gtggtcgatg
     7741 cagcacaagg aatcgaagca caaacattgg ccaatgttta tttagcgtta gacaatgatt
     7801 tggagatcct gccagtcatc aataaaatcg acctacccgc agcagatccc gaacgtgtcc
     7861 gtcaagaaat cgaagacgta atcggaatcg atgctagtga agctgtatta gccagtgcca
     7921 aatcagggat tgggattgaa gatattctcg agcagatcgt ggaatatgtt cctgcaccaa
     7981 gtggagactt agatgcaccg ctaaaagctt tgatctttga ctctatttat gatagttacc
     8041 gcggtgttgt tctaaatgtc cggatcacgg acggtatggt caaacccgga gacaaaatca
     8101 aactgatgag taatggcaaa acatttgatg ttacagaggt cggggtcttc tcgccaaaag
     8161 cagttgctag agactttttg atggtcggtg atgtcggtta tatcacagca agtatcaaaa
     8221 ctgtccaaga tacacgagta ggggatacag taacgttagc agacaatcct gccgctgaag
     8281 ctttaccagg ttaccgaaaa atgaatccga tggtttattg tggtctttat ccaattgata
     8341 cttctcgtta caacgatttg cgggaagctt tagaaaaact gcaattgaat gatgctgctt
     8401 tacaattcga accagaaacc tctcaagcat taggttttgg gttccgctgt ggattcctcg
     8461 gacttcttca tatggatgtc gtccaagaac gcttggaacg ggaattcaat ctagaattga
     8521 tcacgactgc accatccgtt atttaccatg ttaataaaac ggatggtacg actgctacag
     8581 tagataaccc ggcagatttt cctgaacctg taacgattca ggatgtagaa gaaccattcg
     8641 tcaaagcaca gatcatggtc ccaaatgatt ttgtcggtgc agtaatggaa ctttctcaac
     8701 gaaaacgggg agaattcatt acgatggatt atttagatga ttaccgagta aatgtcgtct
     8761 ataatattcc gctttctgaa attgtctttg actttttcga caaattgaaa tcaagcacga
     8821 aaggctatgc ctcattggat tatgaaatgt ctggttatca aaaaagcaag ttagtgaaga
     8881 tggacatctt gctgaatgga gaaaaagtcg atgcattaag ctttatcgtt caccgagact
     8941 ttgcctatga acgtggaaaa gcaatcgttg aaaaactgaa aaaactaatc ccacgccaac
     9001 aatttgaggt gccaatccaa gcagctattg gacaaaaaat cgttgcccgt tcagacatca
     9061 aagccttgcg taaaaacgtc ttggctaaat gttatggtgg agatgtttct cgtaaacgta
     9121 aactcttaga gaaacaaaaa gaagggaaga agcggatgaa acaaatcggc tccgtcgaag
     9181 ttccgcaaga agccttcatg gccgttctga aaatggatga gcaagatagt gtgaaataac
     9241 ttataatcaa tcgaaaaacc tattaaataa acctttttga gtagatgcaa cactgaggat
     9301 tatctgtata aaagatttta aactgtcatt tggttatcac ggcgatacta aaattaacaa
     9361 aattggaata tttttcttga agatttttag ctgtcagtat atgatgaaag catctcatag
     9421 gaggtgcttt tttcatggtt gataattcaa gtggaagggt tcttaaatct ctaagaaaag
     9481 agaaaaaact atctcaaaaa aagttagctg atttagcagg tatttcacaa agtactttag
     9541 ttaaatatga aaaagggtca agaaaaatcc ctaaagacgt agataatact ctaagcaaga
     9601 ttctgaatat agaaactcta ataaaagatg aggaagataa gattgagatt ctcatcggta
     9661 aactaattgc atacagagat atgaataagt tacttaacaa agagttagct gataatatag
     9721 gaattagtga agtattactc agttatgtgt taaatagaaa gagaaatccg tcaaaagaaa
     9781 tgcaaaaaaa aatcgatatt tttctattaa gtaatgaaaa agaaattttg aaggagatta
     9841 atcgtgatag tgagatattt agtttatcaa aagacgataa aattgttatg ggaaaaagaa
     9901 tacgagaagt ccgaaagaac cgggaagaga cgcttgaaaa atttggtaaa aactttacca
     9961 tctatacagg caaaaatgtt ataagtagat gggaaaaagg tattaatatt ccagatattg
    10021 aaaaactaat gaatattgcc tatcttggta aggtcactgt tccttacctt atgtacggag
    10081 aagattataa gaatatactt ccaaaagatg aaagagttag cgattttaaa aagataaatt
    10141 cttttagtat gggattaagg atgaggaaaa ttaggaaaga ttattatcta gaaagggagg
    10201 agtttggaaa gcttttttct ccttcaattt caaaatggtc catagatagg tatgagaatg
    10261 gtagagatat acccaatact aatcgaatta tccaatatgc ttatatagga aatctttctt
    10321 tagaattttt gatttacggg atataaaaat agtttacgtc gatacttatt aagttatcga
    10381 cgttttttat tatagaaaaa atttataaaa ggaggtttct tgatatgata agaaacaaaa
    10441 agggtttgtt gaaagttgct ttactggcag cttttttcta agtaactttt tgacaagctc
    10501 aatggcaaca caagcggaag aagtaataca agaactaact gttatgaata acagctagat
    10561 aaaatgggct atgtatgtta tccaacaatt tagtaatcac tagaagccta gcgaaagttc
    10621 gcttgataag ttatagatga gtattgattt gtagtgtttc caaatcaaaa gggtacagag
    10681 ccacatgaaa tatgttgtcc tctgcagcgt caggatggga atccatacca tggagattcg
    10741 gagatctgtg agggaatact tacgtggtta atatgtcagt tgtgttggac gtagtaatgg
    10801 tcatagtggg tagattgact gaattcttat atgagaaaat gaaaccaaca agaagatagg
    10861 gttatttata cagaattggg gatcttttct ggaaaagtaa tcggataatg cgagattcgt
    10921 acggtctttt ttaaatttct ctaaaaaaaa cggcatcgaa tgggttgggt gattgataag
    10981 caatgaatca tagactgata tagaataaaa aatgattcta tttatctttt tacatctatc
    11041 gttttttcaa gtagccgcaa attatatcag cattcaacaa tttttgtata caagttctag
    11101 taaaaggatc tgaagaaaaa gttggttgtt gtgaagataa aaaaagaatc aggttttact
    11161 gagtttttct tcaaaatttg tgttattagg ttaggacaaa gataagaaga agtatgaaga
    11221 tttaaaaaag gaatgaggaa aagatagtaa tttccgatta ttggagtttt tttatttata
    11281 aagaagaagc gaataagttg tgattcctag atttcgcgcg tgattaatta atgagcaaaa
    11341 aatgcggaaa gttttagtgt ttaatacacg aggacaaagt ttcttaggta ttgaaggcac
    11401 acgtgtaacg gttgatgggt ggtgtgattg tttcgaggtt ggaaaacatg ttgaacttat
    11461 ggcatttact gagctaaaag ataagaacaa cgtggaattt ttcgagagtg atattggttg
    11521 ggacgatcat gaagaagttt atggaaaatt tattttgcgg aagggaaatt cttatacgaa
    11581 tggggaagca taagtagcga tttgttagag atcattgata ggattgaaat tgtcgggaat
    11641 atttatgaga atctagagtt atttgaggtg atagaatgag tgatggtttt gaacaagtat
    11701 caataattaa aaatcaagtt cgggagattc ttagaaaaaa aggctatttg gtagatagtt
    11761 attttgaagg agattacgaa acatgggtag gtgtctatgc tcgtccagag aataaaccta
    11821 cctatttaga tcctacgacg tcagaagatg cctatttaca aaataggtat cgagtagatg
    11881 gttttaagca agactttgcg gagtggtttg aatgggaaat tgaaaacgga gaagtaaaag
    11941 aagaataaga gtacttgttg tatcaaaata agccagtaga acaaccaaac cagtagctag
    12001 taatgacaat aatactgagg aatcggatca ttatggtatg attgatattt ctcttccaga
    12061 agatcttaag ccagatcctg taacaattgg caatagtgtc atgatttttg attcattaga
    12121 tgaagctatg tcatatggtt ggaaagaaat taaggatgaa ggtagtaatt ggtatggcat
    12181 gagatttggt gatggtgaag ttgccaaaaa agctggtatt ccaattggga aatggacagt
    12241 tgatttccac gaagcaggtt ctttttttgt tctgtaatta tagtgggagg gtaaaatcat
    12301 tgatgaaatg tgttcagata agagcgccag ataaaaatag aagagacagt tcacgtcgtt
    12361 atcacttttg gtaacggcgt tttgttttat tatataaaat tctcaggaaa aggaggaatt
    12421 cttgtgtatg caaaaacgaa aatgaattgg ttgaaagcac tcgcattggt ggctttttct
    12481 caattctgtt tcttggtggg aactgctcaa gcacatgcag aagaaacgac ggctatacaa
    12541 cctacgatgg aagccattgc ccaagtcgct tctgaagtga cagaagtcca accagcagaa
    12601 gccggggtcc cagctggtga agccactgtt gaacaggccc ctgtgacaga agcgccaacg
    12661 gtcaattcca aaacgactct cgatcagacg attggggaaa cacaacaccc agggaatagt
    12721 cctggtactt cacaaccagt tgaagaaact gcctcaatta caacccctgc ttcagaaacg
    12781 tccactctag aaggagaaaa agctgccgaa gcaacgcctt ctgtgcagcc agagtcttct
    12841 gagaaacaag cggaagaaaa gaaacgaata gaagaaacag tggaacaacc agcggctact
    12901 gctgacaaag aaggaaaaaa tgaggaaaac gttgaaaaaa caatctcttc aaccgcagct
    12961 agtcaaaatc aaccaacgaa accaaacaaa gaatctacgg ctagtattga aaatcaaaag
    13021 aaaaaagagc agaacattcc aaaaacaggt acacctacac tgttagtaac agtaccaacc
    13081 aatcgttcgg ttcgtgttcg ccgtgcaacc aatgcagctg ttttaggtgg tcggaaaatt
    13141 gattggggcg aaaatattgg tgttacttct agtccgaaca atctaaactt cactttaaat
    13201 tatatggttc gttttgagat taatggacaa gtggcgtttt gtattgagcc tggtgtctca
    13261 gccgcagaag gtcaagttta tacagctact gctttgaaaa actatttgaa aaattcaaat
    13321 gtacggaaaa aaacaagctt gatctcttat ttaggatatg tcacaaataa agacaaatcc
    13381 gatgaacaat acattgcggc acaatttatg atttgggaag ctttgggcac aaaaatccca
    13441 atcaaaaata tccgtgttga ttatgatcgt cgaaaagcag aaattcaacg tttgattaat
    13501 aactttggtg aaaaagcaac ttttaataat caaacacata cggtaaaaat tggcgaaacg
    13561 ttgaaagtaa agaataaaaa tgattttttg aaatatgtga aagacattca aaaaccaaaa
    13621 ggtgtcaatg tatctattca cggcaatgaa gtatggatta cagtcacaaa agaggcaccg
    13681 gataaagcaa aaattcaatt gaataaaatt aaagttcaag gtgcgccatt agcctatcgt
    13741 cgtccaggag ctcaaacaat tggtgtgtta catccaacag aacctaatgc gtctgttatt
    13801 aatttacaaa ttctaaaaca aggaaacatt cgtgtgttta aagaagatgc ggaaactggt
    13861 gtgaaagcac aaggtgcagc ttctttagaa aatgcggtct atggtttgta tcaagcagat
    13921 gggaaaaaga ttaaagaaat taccttgaaa aaaattaacg gcaaagtaca ggcagaaatt
    13981 aaagatcttg atcctggcaa atatgtttta aaagaaatca aagcgccaaa aggttatgtg
    14041 ctttctgatg aagcaattga aattgttgtt gaacctggaa aaactgtcaa tgcgaccgca
    14101 aaagatcaag tgatcaaagg aaccattgat ttagtgaaag ttgctaataa agatttagtc
    14161 gattcaacga atccagataa caaaccaaaa ttagctggca ttgaaatcac tttgacgtca
    14221 aaaacaactg gcaaagtggt gaaaaccgtt gtgacagaca atgatggtta tgctactttt
    14281 ggtaaaaata ccgttgtctt tgatacgtac attctttctg aaaccaaagg aaaagaaggg
    14341 tataaactat ttgaaccact tgaagtcaca atttctgaaa atgggcaaac tttccattat
    14401 gttttagaag ataaagtaat tgaacaacgt cttaaagtgg tgaaagtgga tcaagaaaca
    14461 ggaaaagtga ttccacttgc taatacagaa ttcaagattt ttgatacttt agccaatcgt
    14521 tatgtcacaa tggagattcc aaatgataca gaaacaacgg atatctttaa aaccaatgaa
    14581 aaaggctttt tcctaactaa cggtacactt acttatggaa aagatcgcta tcgtttggaa
    14641 gaaatcaaag cccccgtaaa ttacgttttg aatcaaaacc cattagtgtt ctcgattacc
    14701 aatgatacag aaccgatcaa agtgatccat tttgcgaacc gtcaagcacg gaaaagtgta
    14761 aaattattca agtatgaaga atggaacaag aagaaaacgc cgctcgcagg tgtgaacttt
    14821 accttgtata acggtaaaaa tcaagcaatt ggtgaatata ccactgatca aaacggggaa
    14881 attttagtga aaggtctagt tgccgatgac tattacttct tggaaaatgc cccattagaa
    14941 agtttccaac caaatacaga taaacaagca ttcacggttg cattcaaaca aaatgcgcca
    15001 gatgatgggg aaacgttagt ggtttctgta cgtaattact tgattccacc aaaactagaa
    15061 acacaagcgt caaataaagc cgatggtgaa aaagtggttg atccattaga aacgatcgtc
    15121 attaaagaca gtgtttctta taccaacttg tttgtcggaa aagaatacac catttcagga
    15181 gtattgatga acaaggcaac gaatgcgcca attttacaag atggcaaaga aatcacaagt
    15241 accttggcct ttattgcaga caaagtcaat ggcacaaaag accttgaatt tgtggttaat
    15301 gctagtgttc tgaaaggtca aacgatcgtt gtttttgaaa aacttttccg tgatggcatt
    15361 gaagttgcag cacatgcgga tatcaacgat gaagcccaaa gtgtgaaagt gaataatcca
    15421 accgttcata ccacggctgt ctttgaagac ggattgacag tcgcagaccc aacaggattg
    15481 tttaccttaa acgataccgt tgaaattacc gagattcttc caggaaaagc atacttggtc
    15541 aaagggatct taatggataa agcaaccggt gaacccttgc ttgtgcatgg aaatacggta
    15601 gaaacgagtg ttggctttat tgccaaagca gaaaatgaaa cggtgatcgt accatttacc
    15661 tttgatttga caggtataaa tggtcgtgaa atcgtcgtgt ttgaaagtct ttttagaaat
    15721 gatgaagaac tagcaaacca taaagacatc aacgacaaag ggcaaacggt acgagtaact
    15781 aacccaagtg tacgtacaac ggctacacat aaacccactg gcttgaaaga ggttaatcca
    15841 ttaacgaaag tcaccattac agacgctgtg aaataccacg atttggtcgt tggaaaaaca
    15901 tacacagtca acggtgttct aatggacaaa gtaacggaaa aaccagtgct agtagatgga
    15961 aaagaagtca caagtacctt aacgtttgtg gcagaaacaa cggatggtac agtcaatcta
    16021 gactttattc tagacgcacg tgcgttgcga ggcaaagaag tcgtggtctt tgaagatctg
    16081 tttagagaag gcgtattatt ggcttctcac gcagatatta ccgacaatga ccaaaccatc
    16141 aaaatcagta atccaaccat tacgacgaaa gcgacgaata aaaatggcgg aaaagagatc
    16201 ttggccttgc caaatcaaac tgtgcttgaa tgggtcaaag tagacggtct tgcgattggt
    16261 caaatctatc gcttaattgt tcaaggctac ttaacaccag acggcacacc atttgaaggc
    16321 actcatgcgg aaaaagtctt tgcggccgat aaagaaatga tggaattctt atttgaattc
    16381 ttagtggacg gtcgcaatct tgcaggaaaa ggcttgagtt ttgcggaatg gctccaagcg
    16441 aaaacggaag atgagaaaga aacgtttgaa gaagtggcga aacacaatgt agatctaaca
    16501 aacaaagatc aaacggttca aattgtggaa gcgccaaaac caccgcaacc agtgaaacca
    16561 gaacaacggg ttaaaccagc tactgttgta ccaatgttgc caaaaacagg tgaacaaaca
    16621 agtagtatac ttatggttct tggattagta gtattagtca ttgctttagg tataacactt
    16681 tataaagtac gtaaagccaa agattagtca tgaggaacaa gcggttagga tttttaaccg
    16741 cttgtttgat tgtaatgtag aaatatttag aatttatttt ggtttttaag aggaggaaaa
    16801 actttatggc agaaaaagca ttgcgtatga agaaagagtt tttaggtcaa gtatttttat
    16861 tgggttcacg ggaacctaat ccaccaaagg aaattaatgg tgtgtctggg cgatatgttc
    16921 aattacaagc agagtgttcg cgagaagaac agcctgcttt ttttgtttat ctcacagctg
    16981 aagaggataa aagattacga ttaccagaaa aagaggtgcg tatttcattt gaagaagctt
    17041 attttgtttt tcgtaaagaa cgagtgaata gtcgtgaaac aaatgatatg ggacaaacag
    17101 agcgtcgaac aactggctat caagatgttg gtgtcttttt aactggtgta aaaattgggg
    17161 aggttttgta agatgggatt aaaatttcca aataattatg acattacaga agcattagat
    17221 gaaacttgtt taggagccgt acgctttggc gcattagaag agtctgatcg tgtatttgat
    17281 aatgaaacag gcgattatac gggagaaatt cgatcacagc gagcacaatt agtttttgga
    17341 aatccagatg aaccaaagat tgcacgacga gttaaatttc cagcagatgt agatttgact
    17401 ggttttaaac gacgtgatcc tgtgaaatta attgatccag aatatcatgt ggtatatatt
    17461 ccagcacaag atggcaatcg tgaatattca gatttagtga tttatgcgaa aggattagag
    17521 aaagtagaag ctccaacaaa taatcctaat atgaaacaat cacaagaaaa aggtttagat
    17581 caaaaacaac caagtgttga ttctaaggaa aaagagaaaa aataaggttt ttttgaaaga
    17641 ataggaagga gtcttcctat tctttttatc taagatagaa ggaaggggag attttgaatt
    17701 cagataaaac caatcaaata ctttgagaga ttgtgcttgt tatcaaaata tccaacaatt
    17761 gattgagtat tttggagaca atccaacgat ttttgtacaa acaaatgcat atatttgtgc
    17821 ggttattggg cagtgtatgt ttttacgtga tcttgttcgt ttttcattac cgtaccagtg
    17881 gaaacaagat catcaggaag tgatttggat tcgtatagta cgaatatgta atatgtgtgc
    17941 acacccttat aaaaatcgat taaatctttt tcgattatgt ttttttacta aatgtgatat
    18001 tcctgtatta gttgcttttt gtcaaaagtt tttagcacag gaaaagtaaa aacagaaagg
    18061 aggaatgtga caattgaaag ctcatttttt caagtatcgt ggtcggcgaa ttcgctattt
    18121 caatcgttat ttatggtttt gctattatgg tttctttttg tttccatttt tgattctgtt
    18181 aggttggttt agttggaaag tattttatcc acgtttaagt acgttttcga attggcaaat
    18241 gtatcttatt cctggaataa gtattgtgtt gtatttgttg ctggttagtg ggttaatttt
    18301 agggttgatg cattggtcgc gactaaaaga aggatatttt gcctctgttt attggcgtca
    18361 gttacttgtt cgaatgttaa ttgacaatcg gtttgtttat acaaagaaac aagttagtga
    18421 gaaacaaaca agggaaaaaa tgagattacc aaaagtttat ttttatcaaa agaaacaaga
    18481 attagtggtt tcttttcctt tagatggtgg gcgttttcac gaacgatttt taaaaatggg
    18541 tcatttgtta tctgaagtat ttttgcgtga tttaattcgt gaagaacgag aaaaagcccg
    18601 aatcaattat gtattccttt cagattctgg tcggattcct attgatcaat gtatagcaga
    18661 aaatggacgt attcatttaa tgaaaacttt ggattgggta tatgatcaat caccaaatat
    18721 gttgattgct ggtgcaatcg gtggtgggaa aacctattta ttgtattctt taattcaagc
    18781 ttgtttaagt gttgggacag tagatatttg cgatggaaaa gcggcagatt tagcagcatt
    18841 aggtgacgtc gatgtattca aaggacatgt gttttacaaa ataaatgaag aaatgattat
    18901 ttgtttacga aatgcattaa aagaaatgaa tcaacggtat gcctatatga aaaccatgaa
    18961 agatcctaaa tatgaaccag ggaaaaatta tgcttattat gggttaccac cgcattttat
    19021 cttttttgat gaatgggcag ctttttatgg ttcgttggat atggaagcga gaaatagagt
    19081 cgataaattg attcagccat tagtattaaa gggtcgtcaa gcagggatgt acattatttt
    19141 agcaatgcaa cgaccagatg cagaatattt tccaagtggt gttcgggata atttaacatt
    19201 ccgtatttct gttggtcgat tgtctcccat tggttattta atgacttttg gagaagattt
    19261 taaaaataag ccattttaca acaaagatca acctggtcgt ggatatgctt ttccaggatt
    19321 tgatgtacct gtagagtttt ttgcgccatt tgtttacaaa gattatcact ttatcaaaga
    19381 atttgagaaa tttactgaaa tgattgtcta tgattttaca gtaaaagagg atagtcaaga
    19441 tcaaacgatg ggagatgata atcagttgtt acaagaacaa ctagagcgcc aaatgtattt
    19501 agcaaaagat ccaaaaggaa caagtttttc tgatgcgcat attttgtaat caactaagga
    19561 ggtgaagtta aatggcagag agttgttatt atagagtagc tacagcaata cgaatgatta
    19621 atccttctct ttcttccaga acgttttatg attggttgaa tcgtattgaa caagtaactg
    19681 attaccgctt tttgcgcaaa gaacgtgttt ttacaggaaa agttattaat caagtattgc
    19741 taacgaaaaa agatatagaa cgattgactc gattgtatca ttatcgagta gatttagaag
    19801 aggatttaac tttgtcaatt tatcgcgttt tttctcctga aaaatatagt gaaataacaa
    19861 aattagatca tttaattttg tagttattat ctatcgggag ggctctagac gtagtctggt
    19921 agccgcactc tgcctggcgg tgcgcattaa ggctgcgaag catgggccta taatgcgcac
    19981 tcgctagcag cgtgtgcagc tcacaaagag gtcgtgcgct tgcgcacggc ctctttgtgt
    20041 aaagcccccc gatatataat atggggggca gaaataacat aacacataga aaaaagtttt
    20101 ataaaccctt ttgtatcaag tgattatcta aggtcagcag ttgtactact tttcccaaaa
    20161 aacgaggggg actagttgat atatcggtgt ttctaaaggg ttaaaaaaaa tcgattttgt
    20221 tattttataa aaggagcgag aaaatgacac cgattgagct aaaaagctac agaaagaagt
    20281 taagtgcaat tcataatata agtggagata aaaagccgct atcacaagca agatttgcgc
    20341 aattgatggg ctgctcaaaa gattacatta gaaagatgga acaagggaaa agaaaaatcc
    20401 cagaggattt tgaacaacga atcaaggaac gctttcgatt gtatatgaaa agcggaatag
    20461 atcttcgagc aatgattgat tatttgcgtc tttccttttt tgggacagac tacaaaacag
    20521 tgattcaacg tgtattacga atggatctga cagaatttga aacacgtcca acgggcttgt
    20581 atctttttga ctgtctttct gttcgtggga atatctgggt gttctggcat tcaaaacgaa
    20641 ctgtaacaga aaatgtactt gtgcaatttt caggacaagg ttgccgggaa tatgaggaag
    20701 tcatgcgtga acaacaaaca gattggcgaa catggctata ttccatttgg aaaaatcaag
    20761 gaaagttgga agctggttat acacgtgtgc aatgtagccg attcgatatt gcgttagatg
    20821 aactttgggg attggaaacg gaagaacatt tcgatctctt tgaattacta gaaaaacaaa
    20881 aagcaggttt attagaaatg gactttaaga gattccagaa tattggcggt tgttttttag
    20941 atgatggcta ttttcgaagt gaaggattat ctttgtattt tggatcgcga aagagtcctc
    21001 tcttttttaa tttttatgag aaacgttttg aaattgcgaa ccgagaaaaa atttcagaat
    21061 tagaagcatt aacgaaatat ggtatttata atcgatatga gttacggtta gccaatgaga
    21121 aagcaacaca agcagtagaa gcatttattt taggtgattc accgaaacgt ttaggagaga
    21181 ttggcgttgg tttaattaat gcaaaactta ttgtttggga tagactggaa agtgatgcac
    21241 ggattgttga taaacgatgg gcaaaattat ttcgttcgat ggaagaattg gattttacag
    21301 taaagccaga aaaattctca ttagatcgtt cagaacgctg gtttttgaaa caagtagctc
    21361 cgacattaaa gttgttacaa gaagcagatc gtattttagg aagagacttt gtacaagaag
    21421 ggattgataa tgccgaacta aatgacgagc aacaaaacta tctagaatgg atgggaacgt
    21481 caggagggaa aattgcatga atgcatatac gacaacagaa tggctgcgcc tgttggattt
    21541 aaaggctgca atcgaagcat taaatgaaaa gatggttgat ttgtcttatt ttcgttttcg
    21601 agttccttat attgaacaag cggtaaaagc tggacgttat caggaaaaag agaactggca
    21661 agaaattgca cgcttattag aagttcgtaa gggatacgaa caagaactcg aagagttgga
    21721 atttagccga aggaaaggaa ggctagaatt cattcgcttt tatcgatttt cattgccaat
    21781 acctgcgatt ttagcggtta aaaaaggctg cgataaaatg aagatatatg aaaactgtgt
    21841 ggcagcactt agcagcgaaa aacctcttat ggaagaaatt tcattggtga ctgtcacttg
    21901 ggaaatgagt cgccagccaa cagaagaaca gacatatctt tctttagaag agattgagat
    21961 agaattagag gaaattggtc gctatgccac ttgttctacc tattgtggat cggtcattag
    22021 tattgctggt gtgattgtct aaatgaaaaa aattatgatt tatgttggtg cgtattggtc
    22081 acgtgatcca acagtattag aaaatccaga ggcggtacac tattgcttgc gccaattatt
    22141 ctatctgtat aaagaacgat tagaatcgtt gattcgtcag ttaccatata ctgacagacg
    22201 tttagatgaa cttcttctaa ggtatccagc aatgtataaa cgacgaaaaa atagattgtt
    22261 accagaagag tatcccattg aaaaacatga acttgaaggg cgatttgtgg cttatttcta
    22321 tgatgatgtt cgtatgcgct tagtagaaca acgaatggaa atagaaaatg atcgctacta
    22381 ttttatcaat tattgtaaga agcgtaagta tcaagtaact gatgattttt atgattgtat
    22441 tttacaagat ggggaagtga ttctatccaa aattgctcca agttttcgtg aacttgtgcc
    22501 gattgatttt ttgaaaaaat gtcatttgcg gattcttccg taaaaaagag ctataaaagg
    22561 aaagaaaagt agaagccaaa tcggtttctg cttttttatt ttttttagga aggagaagat
    22621 ggtatgtcac tggaattttt aggagagcca attgaaaagg aagagttcgt ttcgcattat
    22681 atggatttgt ttattcaggt gataaaaaac tttgtgcgat tgatgaattt tatccacgcg
    22741 aacaagacta tctagccgct gaacgaataa aaatcagttt attatgggaa aaattcacag
    22801 atgaaaataa ccgaccgcca gattatcgct atttatcaga ttgtttaacg aataatgtgc
    22861 tttctaaaga atacctattt cacaaacgtg agaaaaatca aatgtcggtt gaacattttt
    22921 gtgattgtta tattgcatta ttgcgtagtc aacagttatt aacagtagaa cgatatggtg
    22981 cagagtttgg ttatgtcttg caagctgagc gtcatagagc tacaagatgc tatgaaaaag
    23041 aagatcaagt gattgatggc tatgaagaat tatgtattcg aaacaatcgc tatttgcaac
    23101 aacgtttata tgaacaattg atgatggatt tttcaaatct ctatagaggg tatgaatgtt
    23161 cgatgattca agaaaggtag aagaaaaggg ggaattttta tgtggcgatt attatttttg
    23221 gtggtatgtg gtatggcatt gttctatgtg tatcgtgtct atctctatcc acgaatggaa
    23281 cgatggacaa gttggtatag agatccttta aaacaacgaa aaggattgaa gcggtatcgt
    23341 tatgaattac ttgttgaatt ttcggatggg cgacaagcac agtattattt ttttgcaaac
    23401 tatccaaaat acaacaccag tgaactattg attcaattat tcaaaaataa tccatatgtt
    23461 gggttagaag aaaacggacg ggagttcttt tatcgttcag ataatattag tgcggcgtct
    23521 atgaacaaaa cacgaattgc ataaaaaatt ggaggaataa tcatgaaaaa aacaacatta
    23581 gcagtcgtaa caacactagg aatttcatta ttttcgtttc aattagggag ttcattggtt
    23641 tatgcagatg aaactgtaga aacacctaag tcagaaattg tagaaacatt gccaggggaa
    23701 ggattaccag atgttgagac agagctaaat ggaggaacaa cggaaaaacc agcagaacct
    23761 gaacaattgc agtcagagaa acctgaaaaa gagccagtga caccagaacc agagtttcct
    23821 gaatcaactt taccgactga ttcgatcgaa gaagaaggtg tgcatagttc aacaacaacg
    23881 gattcaagta tagtcacaac tccggaacaa tcaggaacaa ccagttcttc tgaaaagaat
    23941 actgattcgt caagtacgga acagccaacg atgtcgtcta ccagtgaaga acaaaaagaa
    24001 gaaccgaaac aacctgttga aaaatcagaa gcaattccaa aagaaaataa acctattgtt
    24061 ccagcaaaag aagtcacagt atcggtaacc ccaagcggtg aaattacgac gaatacttct
    24121 caaggaatga gtgtaccgat tgtcactagt aatgtagagg aattgaccca tattccaaca
    24181 ccaaccaccc cattaaaagt agcaggtgat caaacaattg ttggtgtaaa agatggtatt
    24241 ccattaatcc aagatagtga agggaatttg gtaaaggatt tgtcgattcc tgtaaagaaa
    24301 cttccaagtg gcaatattga aatcaaaaca gcagatggaa aaacaaaagt cttaccaaag
    24361 actggtgaag aaatacatgt tgcactttct gttctaggag gagtgttgac aagtattgct
    24421 ggatttgttg gatataaaag aaagagaaaa ggaatagaaa atgagggtaa ttgaatggac
    24481 tatttttcag aaaaacaagc ttatcgttgt caatcgtacg tactagtaaa aacatactag
    24541 aaggagtagc ttatgaaaaa acgacaacta cggcggaaag aaaaagttta tacagagaaa
    24601 gtaccgaagc aaaagaaaat ccattcaaaa aaagcagtga aatggatttg gtgtttggtt
    24661 ttatttcttt gtttttcagg gggcttggca ttttttcaat ccatgaaaac gaaggttcaa
    24721 gtgtcgtttt tgaaagatga aatccaacaa ttacaaaaga aagaagtaca aagtcggtca
    24781 gaattaacgt acacacctga aattgaatcc tttatgaatc gttttgttgc tttgtatatg
    24841 aatacattcg ataatcatga acaagaacaa caacgtcaag aaacattaaa aaaagagttt
    24901 tatgcacaaa actttattca agaaccagga agtgtttcag gtgttcgaaa attaactgct
    24961 gcttcgttta ttggattaaa agaaattgaa accattccaa ctgctagtta taaagtaact
    25021 tatgatattc gctcgatgaa tccagagagt aagaaagaaa caagtgtgac aatcacacaa
    25081 gtactcaatc ttcccttttc tatttcaaac aagggctgtc gtattattgc ttatccttat
    25141 tttacggcac taccaaagaa tacagaaaaa caacgaatga ttacttatga tgacacatta
    25201 tacgaaacgg ttgatgcgtc ggaagcgaaa gatgttgaag gatttgtgga agatttctta
    25261 gataagtatg cgaaaaacaa tgcaaaagat atgtcataca tgatgaaaga acctgaagga
    25321 ctcaatcaaa cagctaaagt tgatcaagta gagatgaaag tattgcgtta taaagaaacg
    25381 aaaaaacctt ttattgtaaa agcaacggtc gtctttcgag atggtgatac gtcactttta
    25441 caaaaagaac aaatgacgat tgtcattacg aaaaaaacaa atcagtatta tgttgaaaaa
    25501 ttaacaca
//
LOCUS       DACDIH010000035        23856 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDIH010000035 DACDIH010000000
VERSION     DACDIH010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.035"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(210..962)
                     /gene="xth"
                     /locus_tag="IV281_001527"
     CDS             complement(210..962)
                     /gene="xth"
                     /locus_tag="IV281_001527"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP8780522.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1075..1995
                     /locus_tag="IV281_001528"
     CDS             1075..1995
                     /locus_tag="IV281_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8780523.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2068..3003
                     /gene="murB"
                     /locus_tag="IV281_001529"
     CDS             2068..3003
                     /gene="murB"
                     /locus_tag="IV281_001529"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP8780524.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3301..4779
                     /locus_tag="IV281_001530"
     CDS             3301..4779
                     /locus_tag="IV281_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8780525.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            5056..7116
                     /locus_tag="IV281_001531"
     CDS             5056..7116
                     /locus_tag="IV281_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780526.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7199..7768
                     /locus_tag="IV281_001532"
     CDS             7199..7768
                     /locus_tag="IV281_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8780527.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7943..8749)
                     /locus_tag="IV281_001533"
     CDS             complement(7943..8749)
                     /locus_tag="IV281_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780528.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8887..10401
                     /locus_tag="IV281_001534"
     CDS             8887..10401
                     /locus_tag="IV281_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP8780529.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(10804..11787)
                     /locus_tag="IV281_001535"
     CDS             complement(10804..11787)
                     /locus_tag="IV281_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP8780530.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(11792..12439)
                     /locus_tag="IV281_001536"
     CDS             complement(11792..12439)
                     /locus_tag="IV281_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8780531.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(12524..13156)
                     /locus_tag="IV281_001537"
     CDS             complement(12524..13156)
                     /locus_tag="IV281_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8780532.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(13172..13723)
                     /gene="coaC"
                     /locus_tag="IV281_001538"
     CDS             complement(13172..13723)
                     /gene="coaC"
                     /locus_tag="IV281_001538"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP8780533.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(13720..14457)
                     /locus_tag="IV281_001539"
     CDS             complement(13720..14457)
                     /locus_tag="IV281_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP8780534.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(14454..15443)
                     /locus_tag="IV281_001540"
     CDS             complement(14454..15443)
                     /locus_tag="IV281_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8780535.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            15701..16243
                     /locus_tag="IV281_001541"
     CDS             15701..16243
                     /locus_tag="IV281_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8780536.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            16260..17345
                     /locus_tag="IV281_001542"
     CDS             16260..17345
                     /locus_tag="IV281_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8780537.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            17357..18163
                     /locus_tag="IV281_001543"
     CDS             17357..18163
                     /locus_tag="IV281_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8780538.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            18164..18991
                     /locus_tag="IV281_001544"
     CDS             18164..18991
                     /locus_tag="IV281_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8780539.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            18988..20061
                     /locus_tag="IV281_001545"
     CDS             18988..20061
                     /locus_tag="IV281_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8780540.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            20489..23185
                     /locus_tag="IV281_001546"
     CDS             20489..23185
                     /locus_tag="IV281_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8780541.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            23262..23750
                     /locus_tag="IV281_001547"
     CDS             23262..23750
                     /locus_tag="IV281_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8780542.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            23762..>23856
                     /locus_tag="IV281_001548"
     CDS             23762..>23856
                     /locus_tag="IV281_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8780543.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVA"
BASE COUNT         7869 a         4101 c         4819 g         7067 t
ORIGIN      
        1 ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt cttattactt
       61 gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc agtttctcgg
      121 tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt tcttaatact
      181 cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac tggacaatga
      241 tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc taaatctttg
      301 ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt gaatcggtag
      361 ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt atcgataaac
      421 ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc agcatttttt
      481 tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag atccccacac
      541 acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc ttcttcccat
      601 tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta acaggttaca
      661 agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg gatatcgata
      721 cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt gccggaatag
      781 ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa atcaatttgt
      841 cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc atgaaaaact
      901 tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga gataaatttc
      961 aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt gtacctcatt
     1021 ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa gtagatgaag
     1081 attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac ttatgcgaaa
     1141 aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa agaaagaatt
     1201 caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt agaagaaaaa
     1261 atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc caaaaaatgt
     1321 ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct aaatgaagtt
     1381 cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg tttcaaccgg
     1441 cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt gatccacctg
     1501 caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga tttgttttta
     1561 catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac aaaataccag
     1621 ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac acaaaatcaa
     1681 acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata tcaagtaaca
     1741 agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca agatgctgat
     1801 ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg cgggtttgag
     1861 ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac agaattgctt
     1921 aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa agatcactac
     1981 cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa taatgaggac
     2041 gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga aagcttagcc
     2101 acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa aacaggtgga
     2161 ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat catcgattat
     2221 tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct gatcgttcaa
     2281 gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat tcatgtcaaa
     2341 ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta tgcagctttg
     2401 gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt cggtggagcg
     2461 gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc tgaagtggat
     2521 cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt attttcttat
     2581 cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt cagtttgcaa
     2641 acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt gcgtcaatca
     2701 aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt aggacactat
     2761 acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg tgcacaagtc
     2821 tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac agattatatt
     2881 gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc tttagaaaca
     2941 gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag tttagccaat
     3001 taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc ctgctctttt
     3061 ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata aaacaaaaaa
     3121 tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa aaaagataaa
     3181 aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt ataggtgaaa
     3241 agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga ggtgtaggat
     3301 atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct agctagatat
     3361 ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac attaggtttg
     3421 tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac tgaattcaaa
     3481 acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg tctgaatttg
     3541 acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc tcgactgtta
     3601 acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca ttttttaagc
     3661 atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca gcatcaaata
     3721 aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt attatttggg
     3781 gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt catgaatgaa
     3841 gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc tgcaacaaaa
     3901 cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg gaatcaacca
     3961 atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat gaagatcatc
     4021 aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat tgatgagatt
     4081 tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg cgcatttgga
     4141 cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc agtggaattg
     4201 attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata tatacgaaaa
     4261 gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga ctttgagatg
     4321 actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc aatattcagt
     4381 acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa agatgaacgt
     4441 ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg tccggtatca
     4501 tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc ttataccgat
     4561 tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga gagtcgaatc
     4621 acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa taaagaacag
     4681 ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa taccatcatc
     4741 agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat ggcagctttg
     4801 tctcacatat ttattagctg atttatgttt ttttcaaaga aaaggtaact agatataact
     4861 agatataact agatatatta cttaacaaaa ttaaaaagta cataagttat tattccgatt
     4921 ttcttgaaac aataaaaaat caaaaaaaat actctcctaa tagtccatct tcttttgatt
     4981 ttatttatat aatgaaatca tcttatgaaa attactactt catgtttaga caagagtagg
     5041 gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac aactaaataa acacgcttgt
     5101 cgtttagata aggaacggtc gtgttctgaa aaacactacg ttaagtttat gatgcgcacg
     5161 atgctgatca gtttatctgt aggtgtctat atgcatgtac ccgttcaggc aacgacacaa
     5221 accatcaagg tcgtacgtac ctctgtttct gagaccactg attcccctat cactattact
     5281 catactttca ctgatgcaaa tggaaaacag ctacttcctg cattgacttc ctataataaa
     5341 gtcagttatc ctccaacgat aaaccactac tggttaattc ctaacaaaac attatggaca
     5401 tcggaaaccg gaagcagtgt gccgctttct atcttttccg atatgactga tcctacaaaa
     5461 ggtgatcagt taaaacagat cactgatatt ttgaatcatg ctttgacaga tataaaggac
     5521 ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt attcacagtt tgaaagaaag
     5581 aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg atctggtaga aaggctgaag
     5641 aacgcagatg gaacacaagg agatatgtcc aaagtaattt caaacaatgg gaatatcgtg
     5701 gatacgacta atacaggaac atctactatt tccttgtcct attttgatcc aattgcttta
     5761 aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg atcaaacagc tatcaaagga
     5821 aaagatcttc acttaagaac aggagagagt tacgccccta aagatcttgt tgaatatgta
     5881 acaaaaagcg atggaacttc aggaaataaa gaagaaatca aaatgaacgg gacagtagat
     5941 actacaactc cgggagaata tcccgttgta ttaacttata tagactcgaa aacatcaaat
     6001 gtggcagaaa caacagctgt agtttatgta gaagcatctg ctgcttcatc gactgtcatc
     6061 actcatttta tagatagtca aacaaataaa gaacttcaag cagaaacaca tatgagtagt
     6121 gtaaatcgta ttcctgattt gcgcctacct gctgaatttt ctgatcgagt gccaaatata
     6181 gatttatcga cttatgaaca aaatgggaaa gtcacttcta tttctgattg gatgcaagca
     6241 aacggattag acagaaaaag tcactggtcg gagatccttg aagtacttca ctcagaactg
     6301 atgaataata ggaagcaatc aggaggggaa attaccttta cttatgttta tataccagac
     6361 aatcgttcat tagaaggtca tgatcttatt gttcatccaa acgaaaaaat aaacaaagaa
     6421 gagttgatcc aatcagcaac aaattcctta ggtaaacccg taccgttaaa ggatgtatcg
     6481 gttacaatca atgaagtacc attggaggat ggttatttga ccaaacaaac aggtgatttt
     6541 acgattatgt atagttatca tgacccgtat tcactccaag aactccatgc tcagtcacta
     6601 ttacatgttt taccattaaa caaacagata gatcaaaaag aacaaaaaag acaagaagaa
     6661 agcttgtcag aaaaaaaaca acaaatggct catgcagttt ctccaacagg gaaagtaaaa
     6721 caaacaagaa gcctagagtt agcaagaata tctagaaaaa tctcagaatc taataaaacg
     6781 ataaggcaag aagctcattt ggccccacaa gtttctggat tggaaaatga aattttagag
     6841 cctaaaataa aacaacaatc agaaaaagaa tttgttgcta aacagtcgga agatcagaag
     6901 gaatcagatg accagaagga aacgaagaaa aaacgcttgt caactattga ttccaccgat
     6961 gagcatctgc agccgcaagt accacttcct agtccgggaa cacccgcacc tggtggaggt
     7021 attccttctt ctgctttagg agatttcatg cgaggtatgg caggaacatg ggtatacgct
     7081 aatcgaaatg aatttgacgc agatgactgg ttctgattta tatctctatc tgttatagtc
     7141 aaataagtag ctgagtcata tgttatttgg cataatggag acgaggaggg atagaaaaat
     7201 gttagataaa acgttacctt acgcagaaat ctggatgaca agaccacttc aacaaaatgt
     7261 tccaatcacg cctttagcgg aaggttttga atttcgattt tatcaaaaag aagatgaatc
     7321 tgcttgggcg gagattgaga cttccgttgg ggaatttcaa acgacagatg aagcacttga
     7381 gtattttcaa caaaactttg ctccttatcc tgaagaactt gaaagaagga tgttttttgt
     7441 agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct tggcgaaaaa aatcaagaga
     7501 gcaagtgtat ccggtttttc attggttagc agtgaaacca gattaccaag gaaaagggct
     7561 cgcaaaagca ttgactgcca gagtcttgca agaatttcct accctccata cggacggacc
     7621 aatctatctt cacacacaga catggagcca tcaggcgatt gctctttata aaaaaatggg
     7681 ttttactttt attgctgaaa atctagacgg cacacctaat ccagattatg aaaaagttat
     7741 gcaggtttta agtaaattag ataaataacg aaatggtgaa ataagtcctc agtattggga
     7801 taaaaaacga aatagtcatt cctcatatct tacttttttg agttaatact atcggataac
     7861 taatggcatg ccatacattc aaaaaagaag gggcagtgac aaaagtattg tcactgcccc
     7921 ttctcttgtt ttttagcatg ttttatcgtt tgattttttt aggaataaaa ttcttgatga
     7981 acgtaaattt ttggtaatcg tttcgactat agaggtaacc aaatacactg aaaacaactg
     8041 ctccaataaa attaacaaat aaatccttca tagtatccat gattcccacg tctaagtaac
     8101 cattttggat gattgtttta tctgttttac catctgcatt ttgactttca atgattgtcc
     8161 gagtgatatc tggaacatga tagacagtgt ttccgtcttt acttaactcc acactattga
     8221 tttgctggat gatcgtatct ttttgcatat cgaattgcag taatttatct gctgaaaatt
     8281 ccacaaactc ccacataaca ccaactgtca tcgaaaaaca gaaggaaaca actgctagta
     8341 ataaaggagt caagttcatg gtttttgtat ttttattgag caaataaaca agggaaaagc
     8401 cgacaccccc agctaaaaat ccgctgaatg catgtagaaa agtatcccac attgaaaaat
     8461 agccataaaa atcacttaac tctcctaaaa ctgttgaact aaaaataaaa gataacaaaa
     8521 tgatttctag catatttgga atctgtatct ttaatatctt ttccagaaaa taagggattt
     8581 caaataaaat taatgtcagt aataaaagca ttgtatttcc ccagtgttgg ttccagacct
     8641 gatccactag cataataacg acgatgatac gtaataaaat atagattccg tttgtgattc
     8701 tacctgcagt ttttgtactg ttcgtaaaaa aggttttcaa atgattcata aaataactcc
     8761 ttcatgtata tccttaatta tactaagaag cgtgtgttta ccaaagctat atgacttaaa
     8821 gaaaaacaga gttgatattg tgaaaaaaat cacttaatat agtataagga gaaaaggggg
     8881 atacaaatga aaaaattagt tgctggttta ttaacagccg ttgcacttct tgctatgagc
     8941 gcatgcggcg cagatcagaa aaatacagcg cagacaagtt ctagtacgaa ggaatctgcc
     9001 gaagtatcat caggcgcttc tgaacaagta tatacagatc caaaagaaat gaaagaatct
     9061 tatgatgtcg tgattattgg agctggtggt gcagggatgg cagcagctat cgaagcaaaa
     9121 gatgctgggt tgaatccagt aattttggaa aagatgcctg ttgccggagg aaacacatct
     9181 aaatcatcaa gcggtatgaa tgcttcagaa acaaaatttc aaaaagaaga aggaatcaaa
     9241 gactcgaatg atgccttcta tgaagaaacg ctaaaaggtg gtggaggaac gaatgataaa
     9301 gaaatgcttc gatactttgt cgatcactct gctgatgcgg tcgattggct agatcaaaac
     9361 ggtatcaaac tggacaactt aacgatcact ggagggatga gtgaaaagag gacacatcgt
     9421 ccgtctgatg gctcggctat tggtggatac ttagtggagg ggttattgaa taacgtccat
     9481 gagcgggaaa tccctatttt tgtcaacgca gatgtgacag acattaagaa aaatggggaa
     9541 acggtcaatg aagtgactgt acatgttcaa ggagaagaac caaaagaggt caccggaaaa
     9601 gcaatcgtag ttaccacagg cggttttggt gccagcaaag aatttattga aaaatatcgt
     9661 cccgatttag ctgattatat tacgacaaat caagaaggat caactggaga tggtatcacc
     9721 atgattgaaa aattaggtgg acaaaccgtt gatatggata aaatacaaat tcatccaact
     9781 gtgcagcagg atgagggggt cttgattggt gaggttgttc gcggagaagg agcaatcttg
     9841 gtcgatcaag aggggaaaag atttgtcaat gaaatggata caagggataa agtatctgca
     9901 gcaattacag cattgcctga aaaatcagct tatttgattt ttgaccaagg cgtgcgtgat
     9961 cgtgccacag ctattgaatt ttatgaccaa aaaggatatg tcacaactgg aaagacaatg
    10021 gaagaattag cagaaaaaat cgatttacca aaagaaacac tagcaaaaac agttgatgga
    10081 tggaatacgg atgttgcgga gaaaaaagat gctcaattca accgtgccac agccatggaa
    10141 catgacttat cgtctccgaa ttattatgcg atcaaaatcg caccaggaat ccattacacg
    10201 atgggcggtg tgaaaatcaa tacgaacact gaagtattag ataaagaaaa ccagccaatt
    10261 agggggttat atgctgctgg tgaagtcaca ggtggcttac atggaaacaa ccggatcggt
    10321 ggaaattcag ttgcggagat cattattttt gggcgacaag cgggcataca agcagctcga
    10381 tttgcttcgg aaaatgaatg aaatagaacg taaataaaaa gaggttgtga aatcagcgtt
    10441 ttgagctaat accgagggtc agctgattga acactgttta gtgaagaggt tgtgaaaaag
    10501 ctgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg tttttaactt
    10561 ggttggactt attaccgcag atgcaagctt ttgaacaccg tttattcgga aaaagagcgt
    10621 gggacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac ggcggaagca
    10681 gaagaaattc cttcggaaat aagctgaaat tcacaaaaat ttgaaaagca attttcgtga
    10741 atttcttctt atttcttgga gttaaacgct tctgtcccag cctctttttt gatcagcatg
    10801 attttattca gttatcttca aaatgatttt tccgatgttt ttgtttgctt ccatatattc
    10861 atgcgcttgt ttgactgcgc tgaattggaa tgtacggtca atgattggcc gaatcttgcc
    10921 attttctaga aaaggcccag ctttttgcat aaaagtttgt gcgagctctg ctttatactg
    10981 atcacttcta ggtgtcaata atgtaccaag cagttggaca cgtttcccaa tcaatacacc
    11041 aaggtctatc tcaggcacga tggtcccacc tagcatacca atcaagaccc aacgtccatc
    11101 aacggctata gaagctaaat tcttttccca gtaagaagcg ccgatgaaat ccaaaataac
    11161 atcgactccc cgaccttcag tgatttttcg tacctcttct tgaaagtctt gttttttata
    11221 gttgatcaat tcatctgcac caagttcttt acaaaaggcg agtttttctt ctgatcctgc
    11281 agtaacgatg attttcgcgt gagaaagctg tctagctaat tggatcgctg ctgttcccac
    11341 accacttgct cctgcatgga tcaagacagt ttcgccttct tgtaattttc caagccagta
    11401 aagtgtttga taagcagtca aaaaaacttc tgagattcca gcagcagaaa catagtctag
    11461 cgtatcaggg agaaggattg cacgatcggc aggcatcaca gcatattcgg cataaccacc
    11521 tagattgact aatccgtata cttttgtccc aggagtaaaa gtggagatat cacttttatt
    11581 ctcaactacc tctccagcta cttcgactcc caaaataggg tagggaggtt tcaaagagac
    11641 tgattctctt ttcataatat ctgttcgatt gacagctgct gtatggactt tgatcagtaa
    11701 ttcgccttgt tttggtttag gaacaggcac ttcaatttgt tctaatacat ctacagaacc
    11761 aggatgcttg attgaaatgg cacgcatgga tctattcctc ctttgcccat tcttggaatg
    11821 ctgtattcaa ctctttgcta ccttcagaaa gtactagagt cgtacgagaa atatccgttt
    11881 cttccactag agcggcaagt acagcagcaa ctgtagagcg agtgatcgtt tttgatgcct
    11941 tctcattagg cttagttaat tgaatggaag taacttcttc atcgttagtc aatgtgactg
    12001 gttgaagaat gacaaaatct aagttagttc gtttcagcca ttcatctgca taatgtttag
    12061 taatataata atctgtcatg gactcaggcc attttgctgg ttcatctgca taaacagcac
    12121 tgaccatcag atagcgagag acatgcgcat tttcagctgc gatcactgtt ttgattgcgc
    12181 catccaagtc tacttgcaga agattctttc cttgagagcc ggcagcaaaa atcaatacat
    12241 cgatgccttt gaatgcttcg gtcatttttt ccacggacca agttaaatca aaagagacat
    12301 acgaaacatt ctcttttttt actaatgttt gggaagcaac atctctcacc cctgcaaaaa
    12361 tttcatggga acttggagct aaatctttta tcaggtgtcg gccaatttgt ccgtttgcac
    12421 ctacaacaaa aattttcatt tggatcatcc tttcgacgtc aaatctttcc attcaatagt
    12481 aacaccaaaa gagaaaaaaa tcagagatcg gctacgatga ctatcagtta gtaagagaag
    12541 ggtgcttgaa gtatcttaac aagacattgc atactgctgc agtcgcaaga cctgcgacga
    12601 ttccttcagg tataccactt gtaaaaataa ccgctagaat agctgaataa acagcattta
    12661 aatttgttcc gatgactttg gcataatctt gctggaataa aaagtagatc atattcatca
    12721 ctaaaatcgt attggtcaat gaaccggaaa gtccagctaa gaataaagaa acggaatctg
    12781 ctttttgttt cattagtttg cgcagacctt tataaacgaa ataaggaacc accccaatca
    12841 agatgcgagg aataaacgcg atcagcaagg ctttccagct tccatgttct gttccgatga
    12901 ctgggataaa cggagaaaag acaaaagaca aaggtgtttg gataactgtg ctacggatca
    12961 tgctgatcag tccaaatgta ccgccaagaa aagcacctag tcttggtcct aaaacgatcg
    13021 aagcaatgat tacaggaata tgcatggttg ttgcattgat tggaccgata gggataaaac
    13081 ctaaaggcgt aacagctaat aaaatcaaaa tagctaaaaa catcgcagtt aatgtaaagt
    13141 tttttgtatt tttcataagt actccttttt tctaagcatt tctttcatta agagtcagca
    13201 tgacctgttc gatgatcgtc tgaatatcag ctaaggcacc ttttccaaaa tctccacaag
    13261 ctaaaagaga ttctcttggt tcaatttcct gatagccgac agatctcaat gtttccagat
    13321 tacgctgagt gatcgggttt tgatacatat tcgtgttcat ggcaggggca atcaattttg
    13381 gtgttcccac tggaagcgcc attgcgactg ttgaaagaag atcgtctgca atgccgtttg
    13441 ctagcttgcc gataatgttt gctgtagcag gcgcgatgag aaacaagtca gcttgttttg
    13501 ctagttcgat atgattgatt acctgcggat ttgattcttg catgacgtct gtatggactg
    13561 cgcgcttcga cagagattgc aaggttaatg gcgtgataaa atgtgtgctg cttttagaca
    13621 taatcacatc tacctgggca cctaatttga cgaattgact tgtaagatca gcgcttttgt
    13681 aagcagaaat acttccagta acgcctagta agatcttttt cattttgatt cctccagtct
    13741 aattttctca gtaatcaatg cagcaatctc agatttactt tgagcttctt ccactgttcc
    13801 atctttagat aagagatagc cgtggtgtgt ctcgccatgg atttccgtca aatcatttgc
    13861 gaaaataaaa tcagttttgt tatttatcag actatttttt gcgactgcca gaagagattc
    13921 ctttggtaca tctactaata gtttgaaacc aatgattttt gcttttggct gccattcacg
    13981 tagataagaa atgatttttg gtgttttctt caaaacgagc acaagatgct cggtgttaga
    14041 agagatcttt gaaacagtgg tatctgtctg ttcattttcc gcaaaccaat tgtccagttc
    14101 ttcttgcgtt gaagaagtgg gtaggttttt tgccagctgt tcttctgaaa aactaaaagc
    14161 aggagtaaaa tcactgactg ccatactgtg gataatcgca tcgtagtgtt cttttttcgt
    14221 tagttcttct aattgtaaaa ataagtccag tgcagattcg atcgtataaa tcgttatatt
    14281 ggagtgtctt ttcggtttca gtgcttgttt cgtagtcacg taatcgattg tggtatccgg
    14341 attggcagct aaataatcag ctaatgcttg ccctaatcgt cctgtcgaat gatttgtaat
    14401 agcacgtact tgatcgattt tttcagtcgt tcctccagct gtgattagaa tcttcattta
    14461 tggtcaccta catttactag tgttcttcct tggtgtgttc cagcaagtag tttagatgct
    14521 gtttcaggca attgatccag agtaatctct tggacgacca gttcatctgc aatctgcaaa
    14581 tccgccatct gttgccataa agaaagacgt tgatctattg gtacattgac agaatcaatt
    14641 ccaatcaact ggatgtttcg cagaataaaa ggcaagacag tcgcgtttag tttgattccc
    14701 ccagcattgc cacataaagc tactgcaccg ttataagaga tcaatggaag aagactgctt
    14761 agctgctcgc cacctactgt gtcgatgaca tagtcgattt gctgttttcc taaagctttt
    14821 gttgtctctg gcagaaattc atcaggtcgt acgacttgca aagcacctag actttttagc
    14881 catgtgacag actcttcttt tctggaaaag gcaatgattg actggaatcc tagtttatgt
    14941 aacagggcta tcgctgtact gcctacaccg ccagaagcac cagtaacgac aactttttta
    15001 tcggaggtca taccttgacg gattaaagcg ttcacacaca acatagcagt aaaccctgct
    15061 gtaccaagga tcatcgcttg acgcagcgtc atattttttg gtagaggaac taaccagtct
    15121 cctggtactt tttgatattg gctataaccg cctggatggg tgacgccaag cccaaaacca
    15181 gtagcaagta caagatcttc ttttgaaaaa cgtggattgt tactttggac gacagttccg
    15241 gataaatcaa tccctggagt catcggatat gttcgtatca cctgaccaga ctcgctcatt
    15301 gccaatgcat ctttataatt gacatcagaa taggcaattt ttaccaagac ttcattttca
    15361 gccaactcag taaatggcag atcactgatt gctgtatgga aaacaggttc ctttattacc
    15421 tgaaaagatc gaaaagcgtt cattcgtgag acactccttt taaaattttc ttttatcata
    15481 ccataaagcc tactctttta ttcttgttct tccgtaaatt tagacaaaaa ttaggtgaaa
    15541 aataaagaat caaacaacta gaatttattc caaaagataa agaaatattc agcaaaaaaa
    15601 attttacaaa gggttgcctt tttcaataaa tttcgtatac taatacgagt ggtagtaaac
    15661 aggaagttta ctattagaaa acttcgattc ggaggaaatt atggaaattg gtgagaagtt
    15721 aagaaatttg cgcatccaaa agaatttgac acaagaagaa ctaggcgaac gcacagattt
    15781 gtcaaaaggg tatatttccc agttggaacg agatttaagt tcaccatcaa tggaaacatt
    15841 cttttctatt cttgaagtct taggtgtgac tccagaagaa ttttttcatc aagaagcagc
    15901 gaatctgcaa gtggtttatt caaaagaaga tcatacggtt tatttcgatg aagaaaacgg
    15961 gtatgaattg gaatggctcg tggctgattc gaatgaaaaa gaaatggagc ctgtcttgct
    16021 gacttttgaa ggtgcaggag aatataagac gtttgaacct tcattatctg aaacgtttat
    16081 ttttgtatta gaaggggaat tagagttaag cttaggagag acggtctatg tggcaaaagc
    16141 aggacagtcg atctattatc aagcgacaaa acaacatcaa ttaaggaatc atctcaaaaa
    16201 acgaacaaaa gtattgattg ttgcaacgca atcatatttg taatataggg ggtaaaaacg
    16261 tggggaaacc aatcatttca tttgatcaag tagtgaaacg ctatgatgat gaaacagtat
    16321 taaaaaaagt aagttttgag atcgaacaag gaaaatttta tacactgctt ggtccttccg
    16381 gctgtggtaa aacaacgatc ctacgtatta tagctggatt caccgaagct acagaagggg
    16441 acatctattt tgaagggaaa cggttgaatg atttgccagc taataagcga caagtaaata
    16501 cagtcttcca agattatgca ctttttcctc atatgaatgt ttttgataac gttgcttttg
    16561 gattgaagat caagaaaatg tccaaatcag atatcgaaaa gaaggtaaaa gatgctttgc
    16621 gtatggtcca gcttcctgga tacgaaaaac gggagattag tgaaatgtct ggtggtcaaa
    16681 gacagcgtgt agcgattgca cgagcaattg taaatgaacc gaaagtattg ctattagatg
    16741 aaccgttatc ggcgcttgat ttgaaactac gtacagatat gcagtatgaa ctgcgggatc
    16801 tgcaacaacg attaggaatc acgtttattt ttgttactca tgaccaagaa gaagcgttgg
    16861 caatgagtga tgagattttt gtcatgaata aagggaaaat cgtccaaagt ggtacacccg
    16921 tagatattta tgatgagcca atcaatcatt tcgttgctga ttttgttggg gagagcaata
    16981 tcgtggacgg tgtaatgatc gaagataatc tagttgagtt tgttggtaag caatttgaat
    17041 gtgtcgatgg cgggatgcgt ctaaatgaac cagttgaagt tgttttacgc ccagaggatt
    17101 tgacgatcac gacaccagat aaaggaaaac tagtcgtaac ggtcgatact cagctgtttc
    17161 gtggtgtcca ttatgaaatc atctgttatg atgaacagca aaatgaatgg atggtccatt
    17221 caacgaaaaa agctaaagaa ggaagcaaag taggattggc ctttgagcca gaagatatcc
    17281 atgtcatgcg tttcaacgag tctgaagaag aattcgatgc acgtcttgac agctacgaag
    17341 aatagggggg acaagaatga aaacaatgcg caggatctat tccgtccctt atattttgtg
    17401 gctcggttta ttcgtgatcg cgcctgtatt aatgatcatt tatcaatctt tttttgatat
    17461 gaatgggcaa ttcacgctga ataattatct atcttatttt acgtcgggga catcgctttc
    17521 tatgacgatc aattctgtat ggtatgcatt tttgatcacg tttttcacgc tgatcatcag
    17581 ttacccgact gcttattttt taacaaaact gaagcacaag caattatggc tgatgctgat
    17641 tattttgcct acatgggtga atctgctgct taaagcttac gcatttattg ggattttcag
    17701 tgtgcacggc agcgtgaatc atttctttga ttttatcggg atcgggccga aacagatatt
    17761 attcacagac ttcagttttc tgttcgtggc aacgtatatc gagattccat ttatgattat
    17821 gccaattttc aatgcattgg aagagatgaa tccttctttg atcagtgcta gtagagattt
    17881 aggcgcgaat agctttgaga ccttccgacg agtgattttt ccgttatcat taaatggtgt
    17941 gaaaagtggt gtccaagcag tatttattcc ttctctttct ttattcatgc tgactcgtct
    18001 gattggcgga aatcgggtga tcactttagg aacggctatc gaacagcatt tcttggttac
    18061 acaaaactgg gggatgggtt ctacaatcgg tgtgatttta atcattgcta tgttcatcgt
    18121 gatgctcctg acaggcgaga agaaaaaagg aggtcgctca taaatgaaaa aatttaaatg
    18181 gtcgtatctt tatttaggga tcgtcttttt attcttatat gcaccgatct tctatctgat
    18241 tttttattcc ttcaatgaag gaggaacgat gaacagcttt acaggattta cttgggaaaa
    18301 ttatgaagct gtttttgaag atacacgttt gatcattatc gtattgaata cgttcatgct
    18361 ggctttctta tctgcattga tcgccacgat catcgggaca tttggggcta tgggaatcta
    18421 ttacacgaaa aaacgacgtg cacggacagc gttgttgagc atgaataaca tcttattagt
    18481 ctctcctgat gttattatcg gtgctagttt cttgattttc tttacactac taggattcgg
    18541 attaggtttt cagagtgtat tgctttccca tattgctttc agcattccaa tcgtcgtctt
    18601 gatggtcttg ccaaaattgc aggaaatgaa cgattcaatg gtcgatgcgg ctagagacct
    18661 tggtgctaat aatttccaag tagtcaaaaa tatcattttg ccttatcttt ctccaggaat
    18721 catagctgga tattttatgg catttaccta ttctcttgat gattttgcgg ttacattttt
    18781 cgtcacagga aatggcttta cgacgctctc agtagagatc tattctcgtg caagacaagg
    18841 aatcagttta gaaatcaatg cgctaagtgc gttagttttc ttgttctcca tgatattggt
    18901 cattggatac tactttatta gtaaagaaaa ccgtccaaaa cggctgaaga aaaacagaag
    18961 agtacgcgag ggggtggcac ggctgcgatg aaaaaattac aatctctgtt tttaggtatt
    19021 ctcatcatta ttgcggtgct gctgcttggt gttcgtcaac ttgaaaaaac aagtggcatg
    19081 agtggagcaa aggtacttac catctataac tggggagatt atatcgatcc tagtttgatc
    19141 acgaaatttg aaaaagaata tggatacaaa gtgaattatg aaacatttga ttctaatgaa
    19201 gcgatgttca caaaaatcca gcaaggtggg acaaactacg acatcgctat accaagtgaa
    19261 tatatgatcc aaaaaatgat caaagaaaaa ctagtacttc cattagatca ttcgaagatc
    19321 aaaggattaa ataatattga tccacgattt ttagacttgg attttgatcc agataatcag
    19381 tattcgattc catatttttg gggaacttta gggatcgtat ataacgataa attctttgat
    19441 gcaaatcaaa ttaagcattg ggatgatttg tggaagccgg aattaaaaga cagtttgatg
    19501 ctgattgacg gtgcacgaga agttatgggg ctttctttga atagtttagg ctattctttg
    19561 aatagtaaga atatggcaca gctgacagaa gcagcaaata aattgaatca tttaacacca
    19621 aatgtcaaag caatcgtggc agatgagatc aaaatgtata tgatcaatga agaagcctcc
    19681 gtggcagtga cattttctgg cgaagctgcc gatatgatgt gggaaaatga acatttacat
    19741 tatgtgatcc caccagaagg atccaatctt tggtttgata atatcgttat gccaaaaacc
    19801 gtgaaaaata aagaaggtgc ctatgatttc atcaacttta tgctagaacc ggaaaatgcg
    19861 gcacaaaatg cagagtatat tggttattct acaccaaatg aagaagcaaa agcgttattg
    19921 ccaaaagaca tttcgtcgga cgaacaattc tatccatctg atgacacgat ctcccatctt
    19981 gaagtctaca aagatctagg atcgaaatac ctaggcatct ataatgacct attcttagaa
    20041 ttcaagatgt ataggagata atggcaaaac tcagtaagaa tggggactta tcaacgttct
    20101 aaagagctaa aaatcgtcct ttttctatct gagttttcca atcttctacc aaaagcggat
    20161 gaaacgccgc tttttttgtg ttattttatt ttttctacca cgtttttcta ccagagaaaa
    20221 gtggacaaaa ccattgtaaa atagggcccg agacgggcta gtaaatctaa gattgaaaaa
    20281 gttacagaag gataaagtaa ttggaaacta tgcaaatata cttaaccctt ctgtaatcta
    20341 ttaataaaac taatatatga atccagttca atatttgtag ctatgaaaaa acataaaaat
    20401 catattagat aaaaactaaa ttggtagata aattattttg tcagatatct gaggcaaata
    20461 ttcgactatt tgtgtttcaa agtaaaaatt ggaatacaaa tatagaatag acatattgca
    20521 gccgatagtt tattatgctt ttagacacat taaactggag gaaaaaatga aaaagagcgc
    20581 taaagatcga gtttttgatg agctgatacg actaacaggg aatatgtctt ctgtcactgc
    20641 cgagaaaatt tcgaagaatc taggtatctc tcgtcagaat gccagccatt atttgacgag
    20701 attagtggaa gataaaaagg tcgagaaatt accgggaaag cctgttttgt ggaagccttt
    20761 agatgaatat gcggttgtag ataatacgga gcaaataaac gaagcctttc attctgtagt
    20821 tggtcatgac ggaagtctga gagaggtcat tcaaaaatgt atagccgctg tcaaatatcc
    20881 gcctaacgga ctcagtgtat taataaatgg agccacgggt gttggaaaaa gttttttagc
    20941 cacaaaaatt tttgaatacg ctatccatga gcaaattatt gaaaaagatg caccatttgc
    21001 tatcttaaac tgtgccgatt atgcggacaa tcccgaactg ctttcggcaa cactgtttgg
    21061 atacaagaag ggggccttta cgggggctga aaaggatacc gatggtttat tggcaacggc
    21121 caacaacggc tatctttttc tcgatgaggt ccatcgccta tcgaaagaaa atcaagaaaa
    21181 gctctttcta tttattgata ctggaaacta tcgtccagtc ggtgaaaatg taaattggca
    21241 ttctgctaag gttcgtttta ttttcgccac gacggaaaaa ggtgaaaatt atttattaga
    21301 cacctttgat cggcgtattc aaattagtgt tatgttgcca acttttgaag atcgccccat
    21361 tcgagaacga ttagagctga ttcaattatt ttttcaaaat gaagcggatg ttttacaaaa
    21421 agatattatt gtttcaagag aagccttgtc tttaatgtta tctcatccct ttagtgggaa
    21481 tattggtaag ctgaagaata tcattaaaat cagttgtgcg gatgtgtata gtcgaacaca
    21541 agaagaacca ttaacgattg ggaaaaatga gatcttgatt cagttaaata tgctggaatc
    21601 cgaagtagaa agcttgccga ttgctagttt attgataaag aaaaatcaaa agataaaaac
    21661 ggatcgaatc aaaccgtttg attatgattc gattttggaa aaaattactc agctgataaa
    21721 tgaaggtaat ttcaatgaaa atttttcttc gataaagaat gaagtacagc atttggtgca
    21781 tcacattgat aaggattctt ttttatcaat gagtagaaaa ttgatgattc agatgtttag
    21841 aaaggtctgg gtatccgttt taggaaaaac gtacggattg acgacgagtg gacctgttgc
    21901 agaaattatt agcagatcct atgtcatttc cccaagctat aagacagact taaccgggat
    21961 aaatcgtaca ttagctgcaa atctacctcg gacgacttac ttatgtaatc agttcttgga
    22021 gcatttacca ccgctctcag aaaaggacga gaactttcta agactgctta ttgtcgtgac
    22081 tttatctgat tatgtagatg aatccatcga gctgaagggc ctgttgcttg ctcacggaga
    22141 tagtacggct agcagcattc aagcagtagt gaatcaatta tgcggcaatt atgtttttga
    22201 agcgttggat atgccagtag agagtaatgt gtcagagatt gtcgaatcga caaggaattt
    22261 cgtgaaacgg caagtgaata cagatagtct agtgctgatc gtagatatgg gctctttaaa
    22321 tcaaatttac acacagatca agaacgattt aaaaggggag ttacttcttc tgaataattt
    22381 gacgacttcc attgcacttg atattggctt gaagatgacg atgaatgcgc catttaaaca
    22441 gattgcggag gatgcagcag acaagtattc aatcaatgtt caatactttg aaggcttttc
    22501 tcaaagtagc aacattatca tttcctgtat gtcgggtttg gggatttcgg acaaattgaa
    22561 agatgttttt cgtcatcatt tatcggatga atccatcgaa gttttcacga aggactacag
    22621 agaactacgt gaattaatcg accaaaatga tgaaacttat tttgacaaga caaaattggt
    22681 catcacgact tcggatcttc cacgttcctt ttcaattcct aatattaatg tttatgacat
    22741 cttagacggt gatggagcag ccaatttgga gcatattttg agtcctgaaa tgaagccggc
    22801 agaatttaac cgcttgattc aggagctagt aaaattcttt tcgttggaag gaattgcgga
    22861 ccgtttgaac tttctgaacc caacgatcgt gatgagtgaa gtggaaacag tcatttctaa
    22921 atatgaaaat tactatcatt tcactttaaa tggccgtgta aaactaaatc tctatatgca
    22981 tacggcattg atgatggaac gtttattctt ggctcggaca gaaaaatctg aagaaccgaa
    23041 ttatgatttg aaaccagatg aagatgaatt ttattatgtt tctagaagta tttttcaatc
    23101 aatggagatg aaatataatt ttaaagtcaa tgcttatgag ctttcgctgc tctatgaatt
    23161 attaggaccg tatattcaaa aataaagtgt ctaaaaaagt gtctaatctg ttttttttag
    23221 gttagacact ttttttaatg tgaattaacg tgattaaacc aatgttttta aaagttggca
    23281 caggaattgc ttatataaaa gtgaacagaa agaaaggaat taaccatatg aagaaaattc
    23341 taatagcgac tcacggtcac ttagcttcag gctttcttag cagtattcaa ttactgacgg
    23401 ggaaggttca agagattacg gtgattaatg cttatgtgga tgagtgtgat tttgaacagg
    23461 agttgaatgt atttgtaaac caggtgaacg agcaggatca gatttttgtt tttaccgatt
    23521 tatttggcgg aagtgtcaat cagaagatta ccaaaacctt tttggagaaa gagattgctg
    23581 taactatttt gtctggcttt aatctgccaa ttctgctcga aatagtattg gcaaccaagg
    23641 aattaacaaa ggaggatatt caggaactgg taaataaatg ccaacaggaa ttaaagatga
    23701 ccattcttga tgaggtacat gtagtaaatg aagaggaaga ttttttttag gaggaagaaa
    23761 aatgattaaa gcagtgcgaa ttgatgaacg attaattcat ggacaagtag cgatgacttg
    23821 gacaaagagt ctaaatttaa ccgggttagt agtagc
//
LOCUS       DACDIH010000036        23608 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDIH010000036 DACDIH010000000
VERSION     DACDIH010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.036"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..508
                     /locus_tag="IV281_001549"
     CDS             <1..508
                     /locus_tag="IV281_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8780544.1"
                     /translation="FQFWLRRTSNPERDSFLFLFGINNGLRMSDIIGLKVGDIRGKSK
                     PIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQGRHITRDRVYQIYADI
                     AEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQAITKRYIGITEDEIGAS
                     LRGFKLGV"
     gene            complement(681..1154)
                     /locus_tag="IV281_001550"
     CDS             complement(681..1154)
                     /locus_tag="IV281_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8780545.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1166..1702
                     /locus_tag="IV281_001551"
     CDS             1166..1702
                     /locus_tag="IV281_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8780546.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1742..2113
                     /locus_tag="IV281_001552"
     CDS             1742..2113
                     /locus_tag="IV281_001552"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8780547.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2397..3668
                     /locus_tag="IV281_001553"
     CDS             2397..3668
                     /locus_tag="IV281_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP8780548.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            3913..4938
                     /locus_tag="IV281_001554"
     CDS             3913..4938
                     /locus_tag="IV281_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8780549.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5232..6341
                     /locus_tag="IV281_001555"
     CDS             5232..6341
                     /locus_tag="IV281_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8780550.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6424..7320
                     /locus_tag="IV281_001556"
                     /pseudo
     CDS             6424..7320
                     /locus_tag="IV281_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7336..8160
                     /locus_tag="IV281_001557"
     CDS             7336..8160
                     /locus_tag="IV281_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8780551.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8192..9502
                     /locus_tag="IV281_001558"
     CDS             8192..9502
                     /locus_tag="IV281_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP8780552.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9650..10909
                     /locus_tag="IV281_001559"
     CDS             9650..10909
                     /locus_tag="IV281_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8780553.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            10919..11791
                     /locus_tag="IV281_001560"
     CDS             10919..11791
                     /locus_tag="IV281_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8780554.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11803..12633
                     /locus_tag="IV281_001561"
     CDS             11803..12633
                     /locus_tag="IV281_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8780555.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12673..14856
                     /locus_tag="IV281_001562"
     CDS             12673..14856
                     /locus_tag="IV281_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8780556.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            14917..16137
                     /locus_tag="IV281_001563"
     CDS             14917..16137
                     /locus_tag="IV281_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP8780557.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16134..17594
                     /gene="gtfA"
                     /locus_tag="IV281_001564"
     CDS             16134..17594
                     /gene="gtfA"
                     /locus_tag="IV281_001564"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP8780558.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17671..18777
                     /locus_tag="IV281_001565"
                     /pseudo
     CDS             17671..18777
                     /locus_tag="IV281_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19099..19308
                     /locus_tag="IV281_001566"
     CDS             19099..19308
                     /locus_tag="IV281_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8780559.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19301..19450
                     /locus_tag="IV281_001567"
     CDS             19301..19450
                     /locus_tag="IV281_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP8780560.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19485..20269
                     /locus_tag="IV281_001568"
                     /pseudo
     CDS             19485..20269
                     /locus_tag="IV281_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(20553..21638)
                     /gene="xerS"
                     /locus_tag="IV281_001569"
     CDS             complement(20553..21638)
                     /gene="xerS"
                     /locus_tag="IV281_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP8780561.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            21972..22205
                     /locus_tag="IV281_001570"
     CDS             21972..22205
                     /locus_tag="IV281_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP8780562.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22202..22609
                     /locus_tag="IV281_001571"
     CDS             22202..22609
                     /locus_tag="IV281_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP8780563.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(23060..23569)
                     /locus_tag="IV281_001572"
     CDS             complement(23060..23569)
                     /locus_tag="IV281_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780564.1"
                     /translation="MKKVLFAVSLVVNIALAGGLFYTITEMNKLDDQRESLAISNEQY
                     KRKLDEASSTNVDTQEAVESSTIVYSNESSETLTNSERSTAPATETVSQKATITIGSK
                     VFTFIVNGLTIEHDSSRTGKMTELSHEFPVIVTTPSATLNGILVQDNNDNNCYIMVDG
                     QTLYRGTYQ"
BASE COUNT         8011 a         3566 c         4494 g         7537 t
ORIGIN      
        1 ttttcagttt tggctaagaa gaacaagtaa tccggaaaga gatagttttt tatttttgtt
       61 tggaatcaat aatgggttac gtatgtcaga tattatagga ttaaaagttg gagatatacg
      121 aggcaaaagt aagcctatta ttgttgaaca taaaacaggt aagcgaaaac cgatttttat
      181 agataatttg agagaagaaa ttttattata tactgaaggt aaagaagaaa atgattggtt
      241 atttccgagt cggcaacaag gtcgacatat aacaagggat cgtgtctatc agatatatgc
      301 agatatagca gaaaaattag gacgagatga tataggaaca catacattaa gaaaaacttt
      361 tgggtatcac tactacaaga aaactagaga cattgctacc ttaatgttta tttttaatca
      421 tagctcgcaa gcaattacaa aaaggtacat tggtataact gaagatgaaa taggagcatc
      481 acttcgtgga tttaaactag gtgtataatt taaaaatttt tataaacagt aaagacaaag
      541 attaaaagct atttttatct agaacgattt tatttcataa agttcgcgga ataaaagctg
      601 gggttactgg tacttggtat aggttcgtgt tatataaact aagtaattaa ataacatttt
      661 taggttggaa aaggatgaaa ttaaaaaaag agaatcaata cggtctcaaa aacgcaaacg
      721 ctgcggaata agaaaaatac tgataactca acgtttagca gactatctaa tagggatagt
      781 ctgctttttt cgtttttgac catacttttg accttctgca aacttccttg cttttcctct
      841 gtagaactca tgaaatctgc aaaacgaatt gccgtttcct ctgtctgctt tttcgcgata
      901 tgggtgtata tatcatccat agtggtttta atatctgtat gtccaagtcg aacatacact
      961 tctttgacag acgcaccaga ttcaaagagc agactacaat gggtatgacg aaaaccatga
     1021 agtgtgatat gttttaagtt attctcttta atgatgattt tcaaattgtg atttacatag
     1081 tctaaataaa ggggcttatt ttcttctgat gaaaacaaaa gttgttcacc atcttttata
     1141 ggtttgattc tcatttttag atattatgcc cgcacgtatt catcacattt ctattgtgaa
     1201 tcgctttatt cgaccgacat ttgactttta tcataatata ctcggtttaa aattactaat
     1261 gaaaacaatt aatcaagacg atcataccat gtatcacctg tttttttcag ataatcacca
     1321 ccgtgtggga actgaactga ctttttttga agtacaagaa gggaacaacc agttttttgg
     1381 aaccaatacg attgaacgaa cgatactaaa agttccttcc gaagcatcat tgcatttttg
     1441 ggaaatatat tttgaaacac aaggcgtttg ccattatgga atcgaatcat tttctggacg
     1501 acctatctta cgttttgaag ggccagatgc aacacgatta gctttagttc ccttacgcga
     1561 atttgaagat attgatgatt atttcccgta tgtcacagac cagatcccta cagagcatgc
     1621 cattttagga attgatgcca ttcaactacg cgtacaatat tccgatgcga ctgaacgaga
     1681 gttattgtcg gtaggctggt agcacaaaga atacgtcagt ttcttttcgg gtcatcaacg
     1741 agtgacaatc ttagaaaatc aagacactca gttctatcaa gaagttcaga ttattcaaga
     1801 tcgtgagaat ccagttgcca ttgaagggat tggtagcgtc catcatgttg cgtttggcgt
     1861 agaaaataaa tctgatttac aacgcatcga caaacaatta caagagcgca attttataaa
     1921 ttcaggaata aaagatcgtg aattttttgt atcactttat tatcgtgacc caaatcaatt
     1981 attgattgaa attgctacag gggaaggaaa cctagatgca aaagcgtatg aaaatcaaag
     2041 tccacgcttt gaagaaattc ctttattttt accgcagtac ttaaatttca ttcgtgaaca
     2101 agtggagcaa taattagcta atcaaatcca tgacgaagag taagaggttg actcataagt
     2161 caaaaaataa agaaaaacgc atcaaatcca ttctaaagaa tgttgatttg atgcggttcc
     2221 tatttttata aactatcatt ttcgactaat aaaactactt atgagtcagc ctctcttttt
     2281 ttcttgattt ttatacctct ttctaggtaa actaaacatc tgccaaaaat accgtgtctc
     2341 attgaaacat tctcaactat ttcaaaataa aggggagtac caaatgaacg agtatattga
     2401 aattatccac gcaagagaaa caacttaaaa atgtcagtct caaaattccc aaaaataaaa
     2461 ttagtatttt taccagcgta tctggttcag gaaaatcatc gattgttttt gatactatta
     2521 cccaagaagc tggtcgtcaa ctgaatgaca cctatgatag ttttacccgt ttatttttac
     2581 ctaaatacag tcgtccagag gttgatgaga ttgataatct ttctacggcg attgtggtca
     2641 atcaaaagaa gattggtggc agtgcgcgct caacgttagg gacgattact aacatcaatg
     2701 cgttatttcg tgtgttgttt tcatgttttg caacacctag tttaggatat gcaaatgctt
     2761 attcatttaa tgatcctagt ggaatgtgtt ctgtctgtca gggaatcggt aagacgattg
     2821 catttttaat aggattgctt tatttttcgg ctattgataa tgctgatttt gtattttctg
     2881 atccatttac cgcagaagtt ttgtctggaa ttgaggaaca ggcaaagaat catggttatt
     2941 ttgtattggt gcataatgtt aattcagcag aagatgttaa aactattcag agaaattggc
     3001 gttttgaggg gttcattgtt gttggtgtac gtgtaaaaga atttttggaa ttaaataacg
     3061 tttttgatgg tcctgttgtt tatattgata cgcatctttc aaaagagaca ttaagagaac
     3121 ttgaacaaca aatgaatcgt ttatttgtaa atacaaatga ttaccaagct agtacattgg
     3181 cagtggaaca tttgattcat tatggtcatg agaaaatagc gtttctttct tttgactaca
     3241 ttgtaggtga accaagtgtt attcaagaac gacatcaagc ttatatcgat acattaaaga
     3301 aatatcattt aacctttaaa tcgcattgga tatataacaa ctctgaattt gagcgaattt
     3361 atcaagaatt agctgaattt actgcaattg ttgttactgg agattattta gcggctaaat
     3421 tagtaaaatt tttaaaaaat aagaataaac gagtgatgga acaattatcg attattggat
     3481 ttgatgatat tacttttgca gaattaatgg atcctgcgtt gacaacaata cgactaaatc
     3541 caggtaacaa gggggcgatt gcttttcacc aactgattgg actggccaaa caagaagaaa
     3601 taaaaaatca agtactacta ttagagggag aattgatttc tcgagactct gttcacaaaa
     3661 tcaaataaaa aagctctgtg aaataagtat agttaacaat aaaatatccg aacgatagag
     3721 agaatgaact gtgatctagc agttcctcgt cgcaaattca ctagaattag cattatttac
     3781 tctaaattgt tcggatattt tttcttgttt ttaattatga ttacagtgcc tatttttaac
     3841 gaaatttgct tattttcaaa atgtatgatt atatgttagt aaacactttc aaatatgaaa
     3901 agaggagcta ttatggtagg aattagggat gttgcaaaac gggcgggtgt gtccattgca
     3961 acagtatcgc gcgtgctaaa tgaggatccg acactttcag taaccgatga aaccaaacga
     4021 aagattgcag aatcggttaa tttttatcat tatgtcaaaa aaagtcccgt tgcaaaaaat
     4081 ttaacaaata ttgcattaat aacgacagtt tctgaagtag atgagttgga agatccctat
     4141 tttcgaacga ttcgcagagg gattcaagtc gaagcagaac aaagcaaagt caatttgaag
     4201 aaaattattc gtttgtcaga aacaccgttg gagcaagacg atttgctaaa atgtcaaggt
     4261 gtgttgatta ttgggcaggt gctatcttct gtgattgaag aagtatcagc aattaattcg
     4321 aatgtcgtag tgattgatga ccctagtatt gaagctgaag tagatgcagt ttatactgat
     4381 ttaacaaaag caacgaatca gcatttagat cgattgtata aaaaaggaca ccgaaacatt
     4441 gcgtttatcg gaggaggacg tgttcgttta gatagccaag gaaataaaca cgtgtcagat
     4501 gatgattatc gaaaaatagc ctatgagacg tggatgaaac aaaaaggatt agaaaaatat
     4561 agtcaaattt atttagaagg ctggaccaca cttgacggaa tgcaggcagc agatttactt
     4621 ttgaaaaaac atgctaaaga taagttgccg actgctatca tggtaggaaa tgatcctatt
     4681 gctgtggggg tttatcgttc acttcaaaaa aatggtctac atattccaga agatatttcg
     4741 attgtgagtt ttgataatat tgaagttgct gaatttttaa cacctgcatt aaatacggtg
     4801 aatgtgaata cagaagaaat tggaaaacta gcagttcgca tggttaatga acgaataaaa
     4861 gaagttagaa atgtttcgat caaagtgatt gtttctaata atattattgt tagagaaagt
     4921 gaaaaaaata tcgattaaaa aagtaaacat ccaaaaaagg gtgtttactt ttttgtttac
     4981 taatattttt agtaaaaaat ctcgacaaaa cattaagtaa acagttatgg tgttgttaac
     5041 aaaaagaaaa cgcattcaaa acaggaggaa ttaagatgaa acatttcaaa aaaatagctt
     5101 taacagggat agctgtttta actgggacat tactcgcagc atgtggtggg ggagatagta
     5161 attctaaaga cgggcaagta actttaagct atgcgagttg agatactgac caagcaccag
     5221 gactaagaaa aatgttagat gaatttgaaa aacaaaatcc tgatatcaaa gtagaaatgg
     5281 agacaacgcc ttgggatcaa tattgggtta aattagaagc tgcaacaacg ggaggtaata
     5341 tgccagatgt agtaaccatg cactctagcg aatcctataa atatatgtca cagaatgctt
     5401 tgatgggact tgatgatgtt atcaaagaaa atgatattga catgggaaac tttactgaag
     5461 gaattgctga tttctatacg cttgaagacg aactatacgc aatccctaaa gatgcttctg
     5521 tagtggggct atggtacaac aaagaattat ttgataatgc aggtgtcgat tatccagatg
     5581 aaacatggac atgggaaaca ctcagacaag ctgcaattga tttaacagat gaatcaaaag
     5641 gcatctatgg ttttgcatca catattggaa cagagactgg ctactggcca tttatttatc
     5701 aaaatggggg agaagtattt gccgatgaca atacaaaatc aggtttgaat accccagaag
     5761 cacaagaagc tttagctttt tatcgagata ttgcacttgt tgatcatgct tcaccaacaa
     5821 ttacagaatt acaagatcct gataagttaa gtcgttttca atcaggtaga ttagcaatga
     5881 tgattgaagg aaactggttt gcaccgggat ttttgaaaaa tgactatcta gttgaaaatg
     5941 ttgcttctgc cgtgttacct aagggaaaag ttaatgcaac aattacaaat ggacttggtt
     6001 gggctgccag tgcgagtaca aaatacccag aagaagtgaa aaaactaatg gcatttttag
     6061 ctagtgagga cgccaataaa atccaagcgg aaacgggggc atcaatccca gcactaaaag
     6121 gctttgaaga cgagtgggct gcttcttcag atacaattga tttgagtgct tttggaaaaa
     6181 tgttggagta tggagtccct cgtccataca ataaagatgg attacgagct gaacaagcag
     6241 aagttcaaat tattaaccgt attctgtctg gtgaagtaac tgttgaagat ggaaccgctc
     6301 aaattgaaaa agaagtcaat gcgatattga gcggaaatta aattagttaa ttatacaata
     6361 tacaggcgaa aggattctat ttcgtctgta tattaatgga tgaataccaa aaagggggat
     6421 attatgaaaa atcgaatttt tggaaaatat cgtaaagatc aagtgatatg gggctatatt
     6481 tttatttttc caacggcatt aggattaatt ttattaaata tatggccagc aattcaaaca
     6541 gcaattttaa gttttcaaaa aacggtaggg ttcggtgata ctgaatgggt cggattaaga
     6601 aattaccaaa aaatgtttgc agacggtgaa gtttttcagt ctttattaaa cacattaatt
     6661 tatgcagtgg tttcgatacc tagtattgtt gtattatcct tgttggtggc agttttaatg
     6721 aatagaaaag ttaaaggatt gtcgatttat cggacaattt attttctacc agtcgttgct
     6781 gcaccatccg cagtcgcaat gatttggcgc tggatgttca ataatgatta tggactgatt
     6841 aataacatgt tggctaaaat aggtctcgat ggaccagcat ggttaacgga tccatcgatt
     6901 gcgatttatt ctattatcat tgtaggagtt tggagtgcta ttggttataa catggtgtta
     6961 ttactagccg gcttataaga aattcctaaa gattattatg aagcagcgga aattgatggt
     7021 gctagtccaa tccagcagtt tttcaacatt acgttaccat tagttacacc aacattattt
     7081 tttgtagttg taacaacagt tatcaatgct ttccaagtat ttgatgttat ttttatgatg
     7141 attactccta atagtaccgc catgtttaaa tcacaatcat tagcctattt attctataaa
     7201 cattcattta ttttaaatga caagggatat ggttcggcaa tcgttatgtt ccttttagtg
     7261 attatattaa ttattactgc aattcaaatg aagttgcaga aaaagtgggt aaactattaa
     7321 ggaggctatc aaattatgaa aaataaattt ttttcaaatg cgccaatcca tatcatgctt
     7381 attttaggtg gtattgcaat ggcattacct tttatttgga tgattttaac ttcagggaaa
     7441 acgttggttg aatctaccca aattccacct acaattttcc ctgagaaatt tcagctaagt
     7501 aactatcaac aagtacttga tttattacca ttcatggctt tttatatcaa tacattttta
     7561 atggttgttg gacgagtgat tggctcggtt ttattttcag caatggctgc atatgcttgt
     7621 gcgcgtctaa gttttccagg gaaaaatatc cttttcagct tagtacttat tcaaatgatg
     7681 attcccggtt ccatttttat tactccacag tatttattag cacaaaaatt gggtatttta
     7741 aatacaattc cagcattaat attaccaggg gtagttagtg cgtttggtac gtttttatta
     7801 agacaatttt tcttaggttt acctgatgaa ttggaggaag cggcctattt agaaggtgcc
     7861 aatatttgga cgatcttttt caaaattatg ttgccgcttg ctaaatcagg attggttgca
     7921 cttggaatat tcacagcgtt gtttgcattt aaagatttaa tgtggccatt gattgtcaat
     7981 acttcaattg ataagatgcc tttagctccg ggattagcaa acttacaagg acaatatgcc
     8041 acgaacttcc cacaattaat ggcaggttcg ttattagcaa tttggccaat agttgtgata
     8101 ttcatcgttt tccaaagaaa attcattgaa ggaattgcta caacaggtag taaattataa
     8161 aactattaat tagtgattgg agagtaagta catgacaaaa attacattta ttggtgcagg
     8221 aagtactatt tttgcaaaaa acgtattagg cgatgcgatg ttaactccaa gtctacaaga
     8281 tgcacatatt gcattgtacg atattgatga aaaacgacta aaagaatcaa atttaatgct
     8341 taaaaatatt aataaaaatt cgaatgaaaa tcgtgctgat attgtttctt attatgatcg
     8401 caaagaagcg ttaagagagg ctgattttgt tatcaatgct attcaagtgg gtggttataa
     8461 accttcaaca gtgattgatt ttgatattcc taaaaaatat gggttgcgac aaactattgg
     8521 tgacaccatg gggattggtg ggatttttag aaccctacgg acactaccag taatgttaga
     8581 ctttgctaaa gaaatggaag aagtttgtcc aaatgcttgg ttattgaact atacaaatcc
     8641 gatggcgatg ttaaccatgg gtgttttaaa agcgacgagt attaagactg ttggtttatg
     8701 tcatagcgtt caagtatgcg taccagaatt gtttgagcat ttaggaatag ccgatcaata
     8761 tgacttaaat gactttcaat ggaaaattgc aggcattaat catatggcat tcttgttgga
     8821 aattacaaaa aatggggaag atttctatcc aacaattcgt cagttagctg cagaaaaacc
     8881 aaatccacat ccagattctg tacgttttga attgttgaaa cgttttggtt actatattac
     8941 agaatcaagt gaacacaatg cagaatacca tccatacttt attaaatcaa aatacccaga
     9001 attgattgac gaattaggga ttccgttgga tgaatactta cgtcgttgtg aacaacaaat
     9061 taatgattgg gaaagcatgc gagaggacat tgtaaataat ggggagttaa cacatacgag
     9121 aagccgtgaa tatgcttcat atatcatgga ttctattacg acaggaactc cgtcagtgat
     9181 tgcaggaaat gtcttaaata aaggcttaat cacaaactta ccgacagatg tttgtgtgga
     9241 agtaccttgt ttagtcgata aaaatggcgt gcaaccaacc tatgtcggtg aattgccaac
     9301 acaattagct gctttaaatc ggacgaatat caatgttcaa gaactgacag tagaagccgc
     9361 tttaacacta gataaatcaa aaatttatca agcagcgtat ttagatccac atctatcatc
     9421 tgaattgtct ttaacagata tcaaaaattt agtggatgat ttaatcgaag cacatgggga
     9481 ctatttacct gaatataatt aaaaagaagc tgaaagagta aggaattttc aattccttac
     9541 tcttttctta gttaaattta gtaaaaatat tgacaaaaca cttagtaaac agttacatta
     9601 gtgttaacaa aaagaaaacg cattcaaatt gtataaggag gagcataaaa tgaagtggtc
     9661 taaaaaaatt attttagctt gtatagcact aggagcaacg atctctttga ctgcttgtgg
     9721 aaaaaatcag gaagatgcaa gcggtaaggt agaaatagag ttttttaatc aaaaaaaaga
     9781 aatgactcaa acgattcagg agattgctaa agattttgaa gcaaaaaacc ctgatattca
     9841 tgtaaaagtt gtggatgtac cgaatgctgg tgaagtaatt aaaacacgta tgcttgcagg
     9901 cgatgtacca gatgtcatta atctttatcc tcaaagtata gaattgaaag aatgggcgaa
     9961 agctggttat ttagaggact taaccgaaga accgtattta gagaatatca aaaatggata
    10021 tgcacaacgt tttgctattg aggatcgtgt atatagcata ccattaaccg ctaatgtcta
    10081 tggtttttac tataacaaaa ctgcttttga ggaaatgggg attcaagcac cagaaacttg
    10141 ggcagaattt gaaaaaatag tagctgagat taaagatcaa aacaaagtac cgtttgcgat
    10201 tgcaggagca gaaggctgga ctttaaatgg ttatcaccaa ctagctatag cgactgctgc
    10261 tggtggtgaa gaggaagcga acaatgtttg gcgtttctca gaagttaatg gaattaatgc
    10321 tgaaagcaaa gaaatgcaaa aagattttaa tcgattagat ttgttaagag aaccaaaagc
    10381 tttgcaaaac aactggcaag gtgccggtta taacgataca gtagttactt ttacaaaagg
    10441 tgatgcgttg attatgccga atggttcatg ggcaatgccg atgattcatt cacaaaatcc
    10501 agattttgaa gtaggaactt ttccttttcc agcagatgaa gcaggtcaaa gtctaacgat
    10561 tggcgcagga gacctagcct tatcagtttc tgcaacttct caacataaag aagaagccaa
    10621 acgttttgta gaatatatga caacacctga agcgatgcag aaatattatg atgtagatgg
    10681 tgcaccgtgt gctgttgagg gcgttatcga gaatactcag gattcaccat taagtggttt
    10741 aactgaactt gcttttactg atcgtcattt agtttggtta gccaaagatt ggaatagtga
    10801 aaatgatttt tataccttaa caacaaatta tttacacaat ggcaatcaaa aagccatgat
    10861 taacgcatta aatgctttct ttaatccaat gaaagctgac ggaaggtagg aaaataaaat
    10921 gaaaataaaa gaaattttaa atcgttactg ggcaatcatt tttgtagcaa ttccaattat
    10981 tttgcagatg atttttttct actttccaat ggttcaaggt gctttttaca gtttaactaa
    11041 ttggactggt ctgacttata attttgattt tgtgggtttg aacaattata agattctttt
    11101 ttctgatccg aagtttatca aatcgattgg cttcacttta attgtgacgc tttgcatgat
    11161 tgctggacaa attggttttg gtattttgat tgcgcgggcg ttgaacgcaa atattaaagg
    11221 gaaaacattc tttagagcat ggttcttttt tccagcggtc ttatcgggcg tggttgtttc
    11281 tttagtattt aaacaaattt ttaactatgg tttaccaaaa attggtagta tgttaggcat
    11341 tgaattttta caaaccagtc ttttaggaac aacatgggga gcgatttttg cagtaatttt
    11401 tgttttactt tggcaaggaa ttgccatgcc agtcatcatt ttcttagcgg gttttcaaag
    11461 tattccacaa gaaattattg aagctgctag aatggatggg gcgaatgata agcaaatttt
    11521 ttggaatatt gaaatcacct acttattacc aagtatttca atggtattta ttatggcatt
    11581 aaaaggtggc ttaacagcat ttgatcaaat ttttgcttta actggtggtg gaccaagcga
    11641 tgccacaaca tctttggggt tacttgttta taattatgct tttaaaaaca atcagtttgg
    11701 ttatgcgaat gctattgctt tagtcttatt tatcttaatt gggattgttt cactggcaca
    11761 attaaaatta tcgaaaaaat ttgaaatcta ggaggtttcc caatgaaaac atcaaaagaa
    11821 aaaaatattt ctaaatactt gcttttatta ttagggggct tcatgatttt agttccctta
    11881 gtttcaacgc tattcggttc atttaaaaca acacccgata ttatgaataa tttctttcgt
    11941 tttcctaatc cagcgacttt ggaaaatttc cgaagattgt tagcggatgg aattggtcat
    12001 tacttttgga attcggcaat catcacaatc attgcagtag ttgctgtgac agcaattatt
    12061 cctatggctg cttattcact agcaagaaaa atgactcgtg ggaaagcata cggattgatg
    12121 tatttatttt taattttagg aatttttgtt cctttccaag taattatgat tccgattacc
    12181 gttatgatga gtaaaattgg tttagcaaat atgtggggat taatcttact ctatctcact
    12241 tatgccgtac cacagacact atttttatat gttggtttta ttaaactaaa tgtgcctatc
    12301 agtctggatg aggctgctga aattgatggg gctagtcact ttacaactta tttcaaaatt
    12361 gttttcccaa tgttaaaacc aatgcatgcg acgactatta ttcttaatgc tctatggttt
    12421 tggaatgatt tcatgctacc attattaatt ttaaatcgtg attctaatat gtggacacta
    12481 ccattgtttc aatataatta tacgggacaa tattttaacg actatggtcc aagttttgct
    12541 tcctatacag taggaattgt cacgattaca ctagtttatc ttgctttcca aagaagtatt
    12601 atagctggaa tgagcaatgg agcagtgaaa taaccaataa attattctca ttataaagga
    12661 gttcataaac ttatgattat atttgacaat cacacacaaa catttcattt acaaaataaa
    12721 aaaattagct atctattagg aatcgaagaa aataattatt taacgcatct ttattttgga
    12781 aaaaagatta cgcactattc tggtgggtat cgctaccccc gaacagatcg ttccttctca
    12841 cccaacaatg caaatgcgca agatcgttta ttatctttag acacattacc attagaattt
    12901 ccaggatatg gacatggtga ttttagagaa ccggcgcata atattaaatt aggaaacggt
    12961 agtcgtgtaa atgattttcg ttatgatagc tacgaaattc aaaaaggaaa gcccagctta
    13021 gcgggattgc cagccttata tacggaagat gatgatgaag ctgaaacgct gattattcaa
    13081 ttgaaagacg cgattagtgg attgacttta cagttgttct acacaattta tcaaaatcac
    13141 agtgtaatta ttcgttctac gcaattagta aataatagtg atgaaacagt ggagattaac
    13201 cgcttagcta gtcaatcaat tgattttcca aatcgtccac tcgagttaat tcatttaaat
    13261 ggtacctggg caagagaacg tcaaatgact agagaaaaaa ttcatacagg gataaaagta
    13321 ttagatagta aacgcggatc aagcagccat caacaaaatc cgtttgttgc tttagtagat
    13381 gccaaaacag atgaatttca aggagaggca tatggattta atttagttta tagcggtaat
    13441 cacgaaacgg tgattcaaaa agatccattt gaccaaacac gggtcattat gggaattaat
    13501 tcatttaatt ttggttggca actaaaaact ggcgactcat ttcaaacgcc agaagtcgtg
    13561 atggtttata gtaatgaagg tctgaatacg ttatcacaga cgtatcatga attgtataat
    13621 caccgtttaa ttcgtggaaa ctatcgttta aaagaacgtc ccacgttaat caacaactgg
    13681 gaagcaacgt attttgattt taacgaagaa aaaatcatga atattgtcga tgatgcgaag
    13741 aacttgggca ttgaaatgtt tgttttagat gatggttggt ttggcggtcg tgaaaatgac
    13801 ttcacttcat taggtgattg ggttgaaaca gaaggcaaac ttgcttgtgg tttggaaaat
    13861 ttagccaagc aagtgcatga taacgggatg aaatttggga tttggtttga accggaaatg
    13921 atttctgaag atagtgaact atttcgagca catccagatt gggcattggg tattccaggt
    13981 cgtgaccgct caattagtcg aaatcaatat gttcttgatt tttcaagaca agatgttcgt
    14041 gataatatct atcaacaaat gacggcgatt tttgatcgtg taccgattga ttatgtgaag
    14101 tgggatatga atcgaaatat gacagaagtc tattctgtct tgttggactc aacgatgcaa
    14161 ggtgaagtgg ctcatcgtta tatgttaggg ttatatgaat ttttagaaaa gttaacaacg
    14221 aattatccaa ctattttatt tgaaagttgt tcgggtggag gcggtcgttt tgatgcaggt
    14281 ttcttgtatt acatgccaca aacttggacg agcgacaata ccgatgccgt ggctcgtctg
    14341 aaaattcaat atggaaccag cttagtgtat ccaatttcaa gtatgggtgc gcatgtttca
    14401 gcagtcccaa atcatcaaac aggaagagaa acgagtttaa ctattcgtgg caatgctgcg
    14461 atggctggtg tttttggtta tgagctagat ctaacgcaat taacttctga agaaaaagca
    14521 gaaattaaag aacaagttgc cttttataaa aagcatcgcc aacttttgca atatggaact
    14581 ttttatcgct tggaaagtcc gtttgaaggc aatgatactg catggatgtt tgtctcaaaa
    14641 gaccaaacag aagccatggt cttttatttc cgagtattag cagaagctgc gcatccatta
    14701 gtcacattga aattagcagg attagcagaa gaagcaactt atcaaatgga agatgtaaca
    14761 tttgctggcg atgaattgat gaatctcggt ttttatattg actcagaact acatggtgat
    14821 tatgcgacac aacgctttta tttgaaacag atatagtagc ttgaatttac aatataagtg
    14881 caacaccaaa agagactcat tccaaacgaa gaaaatatgt tttatttaca cgcatttact
    14941 aaaaagcagc ctgatttgaa ttgggaaaat gaagaagtac gtgaagaaat ctatgcaatg
    15001 attaactatt ggttagataa aggactaggc ggatttagaa tcgatgcgat attaaacatc
    15061 aaaaaaaaac tagagtatgg catctttgaa tctgatggag aagatgggtt agccttcatt
    15121 gggccctgga ttcttaatca gtcgggcatt gaagtgtggt taaaagaaat gagagagcgg
    15181 acatttaaac cacataatag tatgacggta gcagaagccg atgttcctaa tgaacgttta
    15241 gatgaatata ttggtgaaga tggctattat tcgatggttt ttgattttag ttatacggat
    15301 attgatgtac ctgaaactgg tgaatggttt aaagatagcc aatggacatg gacggatatg
    15361 cgtaccaata tttttacgaa tcaattagtt acgcaagata aaggttgggg cgccttatat
    15421 ttagaaaacc acgatcaacc acgttcaatt aataaatata ttccagaaga atggatcaat
    15481 gattatagta aaaaaatgtt ggcgacgctt tttatgttac taagaggtac cccttttatt
    15541 tatcaaggac aagaactagg aatgaccaac atagaaatgg catcattaga agattttgat
    15601 gatgtagcta cgcattctca gtataaacgg gcattagaat atggattggc accagagcaa
    15661 gcattgaaag ctgtcaataa acgaagtcgg gataattctc gcacacctat gcaatggaat
    15721 acgcaaaaaa atgccagatt ctcaacttct gataatgtat ggttaaaagt aaatcctaat
    15781 tatccagaac tcaatgcaga agctcagtta accaatcaac agtcggtatt caattattat
    15841 catcaattaa ttaaattaag aaaaaatagt gtatacaaag acgtgctaat ctacggacaa
    15901 tttctaccag tgaaagataa aaatgaacat attttgcttt atgaacgcca attagaagat
    15961 aaaatcgtat tagttatctt aaacgttaca gcaacagaac aagaatatta tgtagatgaa
    16021 cagtaccaac aagtattaat caataattat gaagatgttt tattagatca aagtcgaaaa
    16081 ttgcgtttac gaccgtttga aagtattgta ttagctaatt atggaggaca attatgaaaa
    16141 ttaaaaacga agcaatgctc atcacctatg cagatagttt aggaaaaaac atgggcgaat
    16201 taaaacaagt cttagacaaa catttacaag gtgttattgg tggtgtacat ctgttgcctt
    16261 tttttccatc gacaggtgat cgaggctttg cgccgagtga ttatacgaca gttgatccag
    16321 ctcttggaac atgggaagat gtggaagcat taggtgaaga ttattatttg atgtttgatt
    16381 ttatgattaa tcacatttct cgtgaatcaa aatttttcca agatttcaaa aaaaaccatg
    16441 agcagtcacc atacaaagaa atgtttatcc gtattcatga atttttccca gaaaatcgac
    16501 caacacaaga agatattgat ttaatctaca agcgtaaaga caaagcgccg tttcaaacgg
    16561 tagaatttgc ggatggtacg acagaagagg tttggaatac ctttggggaa gaacaaattg
    16621 atttagatgt gaccaaagaa gttgtgaaag aatttattcg agaaaccatc aaagatatgg
    16681 cgaaccatgg ctgttcttta attcgattag atgcgtttgc ttatgcgatt aaaaaattag
    16741 ataccaatga tttcttcgtg gaaccagata tttgggaact tttagacgaa gtgcgtcaag
    16801 aagcagcgaa atatgacgtc gaattattac cagaaatcca tgagcattat tccattcaaa
    16861 tgaaaattgc caatcatgat tattatatct atgacttcgc cttgccaatg gtcactctgt
    16921 attcacttta tagtggaaaa tcagaacgtt tagccaattg gttgaaaatg agtccgatga
    16981 agcagtttac aacattggat acgcatgatg gaattggtgt tgtggacgct cgtgatttat
    17041 taacagatga agaattggat tacacctctg aggaattgta taaagtcggg gcgaatgtga
    17101 agaaggtcta ttcaagtgcc aattacaata atttagatat ttatcaaatt aacagcacct
    17161 attatagtgc attaggcgat aatgaccgta gctatctgtt agcacgtgcg attcaagtat
    17221 ttgcgccagg gattccacaa atttattacg ttgggttatt agcaggaaaa aatgatattg
    17281 aactgttgga agcaacgaaa gaagggcgta atattaatcg tcattactat gatttagccg
    17341 aaattgaaga agaagtgcaa cggccagtga ttcaacaatt atttgcactt ttaaaattta
    17401 gaaatgaatc tgcggcgttt gatttggatg gaacgattga agtagcaacg ccatcagaat
    17461 cagaaatagt gattacacgt aaaaataaaa acggtagtga tagtgcaaca ttaacggcta
    17521 acttggatac gaaagaattt atcattgagt ctaaagatat aaaaattttc actagtgttg
    17581 cattaaaatt ttaacctatt ccatagaaca aaaaatcctt tatactagca cttggatgac
    17641 ttatccaaga ccaataaaaa ggactcatac atggataagt ataaaacaaa tacagcattt
    17701 aataaatggt tttctgccat aaaattagga aaactttctg tcaactccca aaaatgtatt
    17761 tttgattaca attcgtatcg taaaaaactg tcttttgaag ctgtgctcaa gctctttctc
    17821 tacgcagtta atgaagaaaa agaaagtttg cgtgatttga gtacagctct tatcgaccga
    17881 tcgctccaac tagaaaccga tgtgtcatcg attagtcata cccaattgtc tcgagcattt
    17941 aatgcacttg aacctaaagt gttagaagaa atttttcaac aactgttaga aaaagttcaa
    18001 catcaaatca aaccaaccaa gcgaaatcat ctgtatctga ttgattccag tacgttttcg
    18061 cttagtttaa aacgtcataa gtgggcgact ttccgtcaaa caaaatcagg tgtcaagtta
    18121 cacttaaact attgttatat ggacgatact acgatgtatc caactgattt taccttaaca
    18181 aatgcccatg aacatgatct caatcaacta ccagtattgg tgaatcaacc agaggcaact
    18241 tacgtgtttg atcgtgggta ttaggacttt gaaaagatgg atcagatgca ttgggatggc
    18301 tatttcttcg tcactcggat taaaaaaaac accaaagttc acgtgataga tactctggaa
    18361 acatcgccag aaacagaaat tctacgagac gagctggttc gtttaggatc taaaacctat
    18421 ctaaccgcca atttccgact agtcactgtt caagataaga acggacgtgt gtttcaattt
    18481 atcaccaatc gaatggacgt ctcttcaaaa gaaatctctg atatgtatca cgctcgttgg
    18541 caaatcgaat tgtttttcaa gcacatcaaa caacacatga ccattaaaac cttcttttct
    18601 caaagtgaaa aaggcgtgca gaatcaattg attttaacga tgatttccgc tctcctcacg
    18661 tttctcatca aattggagac gaaaacggaa aaatcagtgt tccaaatcaa acgattcttt
    18721 cgttatttac tgtttcagcc ctttgaatgt tgtctagaaa aattaattcc cacatgagaa
    18781 aaaagaaatt tggctcgtta cttttggttt tcagttgaat aacagataaa aaattttctg
    18841 gaaaaagtca tcctctgcgt cagcacagtt ttttttgact tattttcaga ggaaatagtt
    18901 ttcagaatat tctaaaatgt tttatgcaac actagtgata ttttttagaa ttagatttct
    18961 aatgtgcatt ttttgagtat taaggagcaa gtatacaaag atttataaag ctgattaaat
    19021 ttgaataaaa atatgcagga acgctactat ttttagtagt gttctttttt atatttgaat
    19081 aaaaaatgtg gtggttaaat ggtggttata acaaaaaatg aattaatgaa attaggttat
    19141 gggaagtatc agtctgagag aatcatacga caggcaaaag caattatggt ttcaaaaagc
    19201 tatacctatt acgaaaatag aagattagga ctagtacctg ttgaatctgt agaagaagtt
    19261 ttaggtatga agatagaggt agaggaggat tttcagaatc atggttaatt ttaaaaaaga
    19321 taaaaatggg aaatatcatt ttactgctag tttgggattc gacaaacgaa cagggaaaag
    19381 agtacaaaaa aacgtagagg ggttaaaaca gttaaagaag aaaaaaagcg tataccgaac
    19441 ttgtttctaa ttatggaaaa gaagcagaaa cttctcaaag tactatgttg tttgaggagt
    19501 ttttttataa attattccta ccttattatc aagggaaagt agaagaaaga acatataaaa
    19561 atcggttatc tacaatcaaa aaaacttttc ttatttcttt aaaatggagt taagagaaat
    19621 acgtccaatt gacattcaaa agtggcaaaa taatttattg agcaaatata aaaattctta
    19681 tgtaagaaac atgtatggga tttttcagat gtcattagat cgagcagttg ttttgggact
    19741 acttgaaaaa aatcctgcaa aagttattgg aaatgtgaag aaaaggaaaa ctacagttga
    19801 tttttggaca aaagaagaat ttacaaaagt tatttcaact ttttatattg atgatttgta
    19861 ccaacatttt ggttttattt gtatgtggtt actttttatg accgttatgc gtttgggaga
    19921 agccactgca ttaacttgga aagatattga ttttgaaaat aaaaacttgc gtgtgaataa
    19981 aacactctat tataagagag ccaaaaccta tgagtttgta gaacccgaaa cggcttctag
    20041 tattcgtaca atcgaactag acgacaatac aattaccttt ttaaaagctt ggaaggagcg
    20101 tcaagtttca ataggaggca tgtcgtttgt actttcttat aatggaacac ctactcagaa
    20161 atatacaatc cgtcatatta tgagaagaca tgcaacgatc gttggtgttc atataatccg
    20221 catacacgga ttaaggcata gtcatgcttc tttgcttata tcacaataaa aagatgcact
    20281 tcgaattaaa gaaaggttaa aacatgctga tgtacaaacg actttaggaa cttatggtca
    20341 tttgtatcta aattcattga gagagataac aaatgatccc acagattata tccaaactaa
    20401 tttttctaaa atcaaaccga cacctattta cgataataaa aaataaatga taaacaaaag
    20461 taggatcaca ccaaaagaaa cacactagaa aaactataat acaggctttt ctagcgtgtt
    20521 gtttcttatt tccgcctatt tatacattat actcataaag catttaatgc atcgatcgta
    20581 gccgcattgt ctatatgggt atataaagtt gttgtattgg tacttgaatg tcccaattgt
    20641 tgggcagtta ataccaatga atttgtctgt tggtagagtc tagtagctaa tgtatgacgc
    20701 agtttgtgag ggctgacttg aattctaaag ccttttgaat attttgctac catccgttcg
    20761 attgcaatac cggatatacg cttggcttct ccttggtaaa ttgctagaaa taaagctttt
    20821 tcgttttctg ttactttata tcgttctttc cgaatagcta gataattagc taaatattcc
    20881 attgcgaatg ctgctatatt gacagcatct cttttatttc cttttctagt tactaccaca
    20941 ttattcttat ctaaagatag gtcatctaaa ttaatattta taacctcgga gagtctcaat
    21001 ccagatccta aaatcagtgc attgatcgcc aggtctcttt ctacatctcg ttgataatag
    21061 atcaaagctc gtttactcaa agtattttta tattcattgg caatataatc tagataattt
    21121 tgtgtatcct tatctagaaa taattttttc tcaatggcat tggcacgtgc agctaacgta
    21181 gttttatctg tgatggttgc tacttgatcc ataggattat tgtctaagta tggttttcct
    21241 agaggattat tggaactttt cgaaagatat ttaaatagaa cctttaatgc cgtgatattt
    21301 ctattgatag tagaagtaga tagacctaag ttattattat ctttgagctt agtttttgtt
    21361 gtctcttttt ttacattttc ttttgtgcga cgtttcaaat gagatttgta caagtttagg
    21421 tcataggcag ttaactgtct tatcgaggtg atagaagtat caggaacgat agactcactc
    21481 agaagccatt cataaaagag cttataattt tttagatatt ctaaagtggt tcgaggtgat
    21541 tgatctttat cctcacgatc ttgaacaaaa tcttttatat aatcgggcat atgaagtatg
    21601 attgtatcta ttttttgata tatttctgta tttttcatgt cattcatcct ttcaataagt
    21661 aggggagaag agtatctcta taattggact ataccaatta tagaatgttt tgtcaattgg
    21721 tatgctctaa aatttattaa tttgtttcaa aaaaagcata taaacaggta tacgtttaat
    21781 aatttaaaat aaatggtata cataaatagg gaaggaaata gaagggagca atctaaattt
    21841 tttgaatttt cttaattaat tatatagaaa gaacaatctt tctatagagt tagacatgag
    21901 agggactatg cttgatttaa aatcaacaag cgtatatact gtatatacaa agaaaaagga
    21961 gggagaagac tatggcaatt aaagaaagaa aatttaggga agtggggaac tcagtagtat
    22021 taactatttc aaaggagttt cttaaagaaa atgggttaaa tcccggagat acggtttttt
    22081 tagatgaaga aaaacttcga gcggcaatta caaaaaaaca agccttatct gatgaaattg
    22141 atttagcaat atctcaagca ttaactgaat atgatactac ttttagagag cttgtcgata
    22201 aatgagtatt cattacctag aagccaaaga tattataaaa atgaatgtaa tccaaataaa
    22261 aaaatactct cctaatgaac cccttggtgt aaaggatgcg aatgctttag aaatgtgtgt
    22321 agggcaactt cgagcctcag cttttggtga agaggtttat ccttcgattt atgaaaaagc
    22381 agctatccta cttatacaat tgataaagaa acatcctttt cacaatgcaa ataaacgtac
    22441 tgctttctta gctacttttg tctttttgaa aatcaatgga cagcttttag ctattgatca
    22501 aaaagatgct gtaaatttag cggtatatat tgctacgtat gataaggatt tcgatcaact
    22561 aaaatatgaa gtgatcaaca ctataaagac acatacgcag cccatttaag gagttactgt
    22621 atgtgtcttt atagggttat tagctgctat agcgaaataa ttgaagtaaa ttacaaatag
    22681 ctataattca aatccaacta tatagaaaac gtaaattttc tatatacttg gatatgctgg
    22741 aatgtttaag agaataatta catttctaga aaatacaata aaaattacag gataattagc
    22801 catatcctac ctcctctagc agttgctcaa aagttagcac taagtaactt cttttttgtt
    22861 gctggtaaca atttatcaat actaaaatag cgtttaagta tctaataaat actaatttta
    22921 aagctcttaa ttattatgaa acacgatata acaatattgc tatatcgtgc tattgtaata
    22981 ttatacattt gaagacagcg tattcttgtt ctaatatcag gaatttttta taaatatcag
    23041 tcatattaag actcaaaatt tattggtatg ttcctctgta tagagtttga ccgtctacca
    23101 ttatatagca attattatca ttgttgtctt gcaccaatat accgttaagg gtagcactag
    23161 gagtagtaac aatcactgga aactcatgag aaagctcggt catttttcct gttctgctag
    23221 aatcgtgttc tattgtcaag ccatttacta taaacgtgaa tactttactt cctattgtta
    23281 tagtagcttt ttgagaaaca gtttctgtag ctggagctgt gcttctttcc gaatttgtta
    23341 gggtttcact ggattcattg ctataaacaa ttgtagagga ttctacggct tcttgagtgt
    23401 ccacgttggt gctgctagct tcatctagct tccttttgta ttgttcgtta ctgattgcta
    23461 agctttcccg ttggtcatca agcttattca tttctgtaat ggtataaaat aatcctcctg
    23521 ctagtgctat attaacaact aaactaaccg caaataatac ctttttcatt cttccttctc
    23581 ttctttctct ttttgtaatt ccttactt
//
LOCUS       DACDIH010000037        23327 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDIH010000037 DACDIH010000000
VERSION     DACDIH010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.037"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..336)
                     /locus_tag="IV281_001573"
     CDS             complement(<1..336)
                     /locus_tag="IV281_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8780565.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESV"
     gene            complement(326..637)
                     /locus_tag="IV281_001574"
     CDS             complement(326..637)
                     /locus_tag="IV281_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780566.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            complement(634..1509)
                     /locus_tag="IV281_001575"
     CDS             complement(634..1509)
                     /locus_tag="IV281_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780567.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            complement(1519..2679)
                     /locus_tag="IV281_001576"
     CDS             complement(1519..2679)
                     /locus_tag="IV281_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8780568.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            complement(2679..3365)
                     /locus_tag="IV281_001577"
     CDS             complement(2679..3365)
                     /locus_tag="IV281_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP8780569.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            complement(3328..4506)
                     /locus_tag="IV281_001578"
     CDS             complement(3328..4506)
                     /locus_tag="IV281_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8780570.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            complement(4526..6220)
                     /locus_tag="IV281_001579"
     CDS             complement(4526..6220)
                     /locus_tag="IV281_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8780571.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            complement(6198..6512)
                     /locus_tag="IV281_001580"
     CDS             complement(6198..6512)
                     /locus_tag="IV281_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP8780572.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            complement(6615..6896)
                     /locus_tag="IV281_001581"
     CDS             complement(6615..6896)
                     /locus_tag="IV281_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780573.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            complement(6901..7245)
                     /locus_tag="IV281_001582"
     CDS             complement(6901..7245)
                     /locus_tag="IV281_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8780574.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            complement(7271..7438)
                     /locus_tag="IV281_001583"
     CDS             complement(7271..7438)
                     /locus_tag="IV281_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8780575.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            complement(7634..7840)
                     /locus_tag="IV281_001584"
     CDS             complement(7634..7840)
                     /locus_tag="IV281_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780576.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            8294..8569
                     /locus_tag="IV281_001585"
     CDS             8294..8569
                     /locus_tag="IV281_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780577.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            complement(9027..9440)
                     /locus_tag="IV281_001586"
     CDS             complement(9027..9440)
                     /locus_tag="IV281_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP8780578.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(9517..9813)
                     /locus_tag="IV281_001587"
     CDS             complement(9517..9813)
                     /locus_tag="IV281_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780579.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            complement(9810..10367)
                     /locus_tag="IV281_001588"
     CDS             complement(9810..10367)
                     /locus_tag="IV281_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP8780580.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            complement(10364..10783)
                     /locus_tag="IV281_001589"
     CDS             complement(10364..10783)
                     /locus_tag="IV281_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP8780581.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            complement(10780..11025)
                     /locus_tag="IV281_001590"
     CDS             complement(10780..11025)
                     /locus_tag="IV281_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780582.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            complement(10985..11341)
                     /locus_tag="IV281_001591"
     CDS             complement(10985..11341)
                     /locus_tag="IV281_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP8780583.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            complement(11341..11646)
                     /locus_tag="IV281_001592"
     CDS             complement(11341..11646)
                     /locus_tag="IV281_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780584.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            complement(11643..11804)
                     /locus_tag="IV281_001593"
     CDS             complement(11643..11804)
                     /locus_tag="IV281_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8780585.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            complement(11801..12103)
                     /locus_tag="IV281_001594"
     CDS             complement(11801..12103)
                     /locus_tag="IV281_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780586.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            complement(12265..12534)
                     /locus_tag="IV281_001595"
     CDS             complement(12265..12534)
                     /locus_tag="IV281_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780587.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            complement(12546..13295)
                     /locus_tag="IV281_001596"
     CDS             complement(12546..13295)
                     /locus_tag="IV281_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8780588.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(13298..13984)
                     /locus_tag="IV281_001597"
     CDS             complement(13298..13984)
                     /locus_tag="IV281_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780589.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            complement(13990..14661)
                     /locus_tag="IV281_001598"
     CDS             complement(13990..14661)
                     /locus_tag="IV281_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP8780590.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(14654..14995)
                     /locus_tag="IV281_001599"
     CDS             complement(14654..14995)
                     /locus_tag="IV281_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780591.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(15173..15871)
                     /locus_tag="IV281_001600"
     CDS             complement(15173..15871)
                     /locus_tag="IV281_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP8780592.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            15958..16428
                     /locus_tag="IV281_001601"
     CDS             15958..16428
                     /locus_tag="IV281_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780593.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(16471..16650)
                     /locus_tag="IV281_001602"
     CDS             complement(16471..16650)
                     /locus_tag="IV281_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780594.1"
                     /translation="MGKFNRALVFSAPLIIYALELWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(16637..16975)
                     /locus_tag="IV281_001603"
     CDS             complement(16637..16975)
                     /locus_tag="IV281_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP8780595.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(16991..17194)
                     /locus_tag="IV281_001604"
     CDS             complement(16991..17194)
                     /locus_tag="IV281_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8780596.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(17209..17985)
                     /locus_tag="IV281_001605"
     CDS             complement(17209..17985)
                     /locus_tag="IV281_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP8780597.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            18284..18604
                     /locus_tag="IV281_001606"
     CDS             18284..18604
                     /locus_tag="IV281_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8780598.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            18622..19053
                     /locus_tag="IV281_001607"
     CDS             18622..19053
                     /locus_tag="IV281_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8780599.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            19183..19644
                     /locus_tag="IV281_001608"
     CDS             19183..19644
                     /locus_tag="IV281_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAP8780600.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            19678..20313
                     /locus_tag="IV281_001609"
     CDS             19678..20313
                     /locus_tag="IV281_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAP8780601.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            20426..21061
                     /locus_tag="IV281_001610"
     CDS             20426..21061
                     /locus_tag="IV281_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780602.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            21360..22499
                     /locus_tag="IV281_001611"
     CDS             21360..22499
                     /locus_tag="IV281_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8780603.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(22568..22641)
                     /locus_tag="IV281_001612"
     tRNA            complement(22568..22641)
                     /locus_tag="IV281_001612"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22605..22607),aa:Arg,seq:cct)
     gene            complement(23030..>23327)
                     /locus_tag="IV281_001613"
     CDS             complement(23030..>23327)
                     /locus_tag="IV281_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8780604.1"
                     /translation="FKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKVEQTKL
                     PIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDYF"
BASE COUNT         7029 a         4579 c         3570 g         8149 t
ORIGIN      
        1 cacactttca agataaaaat agatataaaa attatcgtaa tcggcataga taacgttgta
       61 acgcatacta tcgattacga taaaatattg atctttatta aatttcttgg cgattggatg
      121 aaatggagtc tttactttct tagttaattt cgatcccatc gcatccatag ctgttatatc
      181 actatctctc atggaaagat ttctaaattt taaagaagtg atttctgtat cttctacacc
      241 aatctttttt cctagttcat ttcttttggt agtttgcgtc aaaatcttta accaaccatc
      301 gttgaatgtt tcttcgagtc tacgattatt cgccattcac atcacctgca atatattctt
      361 gtagcgcata atgttgaatg aaacctaata actcactagc gaaattttgt tcaaactcat
      421 ctaaagcacg attccagtcg tatctacatc tttcgattag caatccgtat tctaagcttt
      481 caggagaaaa agaaagtgtt gtactcactt tactttgaag ataaacagca tttttagcta
      541 tcatcttttt aattgactca tcttcttcgt tccaggtaac gtaaatatta tccttcacag
      601 ctattagcaa ttcttcagtc acttgttcag gcgtcatcta accaccgcct taattgcttt
      661 aacatatgcg taagagcatt ttttcttgtt tacaaatgat aaatcttcat caaaaggcgt
      721 agaagtcacg tatctccctt tgaaaaataa atcttcatcg tttgttgtta ctccagcatt
      781 gtgtaagatt tttacttctt taactttttc tattggatca gtagcaaaac aaaagtctaa
      841 ttcctcgtga actttaggac caatattgaa atacatcatg ttccaaagct gtgcccacat
      901 ctcggctgtc cagatttgta tatttgtttt ttgccctcta aggtagcgat atagccgatt
      961 agaatccaga taaacctttt tccaataatt cgctttagga cggttaataa cccactgtgc
     1021 gcctcctgaa ttagtgttta tagtttccaa agattctact gtaacattta caatgtttgc
     1081 catatctttt agaatatttt ctccgttttc acagcttcta atataatcaa gacttagata
     1141 actacagcag tcgctacaat accaaacatc atctttagaa ggcaatttgc gcaaattaat
     1201 tcttttattg aaaatgacat ccgaatcgat atagaaatat cggtcgtcct cacgcgaatg
     1261 atcttcttct aaatatttcc accataaata tggtttaatc gaaggaatat actctttgtc
     1321 gtcccgcaga tcatcgtaca catgaacttc aacaccatat tccttctcaa aaaaaatagg
     1381 aatctgatca tcgtgtctgc tgaaaagcaa tacgatatct ttgattccta gtttcttcag
     1441 attagttaaa caaacttcaa gctcccattt aaaccgattg attgccggct gacaaagaat
     1501 atacttcatt ctgatcacct acgcttgtgt tgtagttgtt gtggttgttg gttttgtagt
     1561 tgtagtagta gttcccaaag cgctaatatc taatacaatg aaactatcgt tacgtttagg
     1621 ttgaccgttt gcatattgtt tagctagata aatgcgttcg tcttcaacaa aatggtattc
     1681 atctgaagct tcaattttta gtgtagatcc tacacccatg aagtaatctg aggctacccc
     1741 aataactgct tttccttctg gcacagccgt tgactgcaaa tctgaaactg gtactggcaa
     1801 tacttgtacg tattctccat tagcagttag tacagtctta gctgggaata ctttagacca
     1861 gtaatcagtt ggattcacaa ttaggaccac atcagaagga ttcacattac gataaatcgg
     1921 atcattcaca ccttcgatat tgaattttga tagtcgcgcc atcaaaccgc ccatagttac
     1981 agcatctaaa gctgtaatag gttctgcttt tttttcagca tattctccgc tagtttgttt
     2041 gctcatgtca cgcatcattc cgactggcat atctttacca gtaccatcaa caattgcttg
     2101 ttctaatgca attctcaatg attctactaa aacagtacgg acataacgat ctaaccatac
     2161 tggacctaaa tcaagcattg ccttacatac aggaatataa cctgatagct tgaactgctt
     2221 catgttaatt acatcaaagc cattatctaa aactttttta acagcttcgc aaagtttacc
     2281 ccaccatgct ggattgactc cacgtgacac aatccattct gttacaccag ttgtgttaac
     2341 aaaagtaatt ttttgcaata gtggatgaga ttgttctaaa tcttcaaata cacgttcaaa
     2401 tacagtagct ggcactaatt cttcgacccc tgcaaaacct tcgtttttca ctacttcgtt
     2461 atagaatttt gtttcttgtg tagttaatac acgctgacca cggttcatta atactaattg
     2521 atcttgattt tttgctgttg cttcttctaa aattttatcc tgaatttcct tagataagct
     2581 taccatagct gcgctaaaag attcttcgtt accatcttta aaagctttca tcaattggtc
     2641 gcttgcagct gttacacctt ttaaattttt aactgtcatt attttacatc tccttgtcca
     2701 aatgttttat ttaatgctgc tgtaaatgca gcaatttttt ctgctctttt ttctttaacg
     2761 tcattcaaaa tttcttcaac gctttgttct tttttagctt cagtacctga gctattttct
     2821 gcatcgataa tttcatcgac caatccataa ctcaaagctg tttctgcatc cataaacgat
     2881 tctttttcaa gaagttcttg caatgcttca tctgtgccat tgaatcgtgt tttatatgaa
     2941 gcctttaccg atttatcaat tgattccagt tggtcagcaa tcgtacggaa gtcatcgaca
     3001 tttccttctc cgtatgtgga agcgcggtga atcatcaatt gtgcattgtt gtagattttt
     3061 atagtatcgc cagccattgc gataattgaa gcagcactag cggctaagcc gttaatcaca
     3121 acgttaactt ttgctttatt tgacttaagt aagttcccaa tagcaatccc ttgaaatacg
     3181 tctccaccgt ttgaattaat tactacttca atttcttctt gatcacctag actatccaaa
     3241 atatttttga ttcccttgtc agtattccct tcaaagaacc aactagaacc aataaatccc
     3301 tgaataaaaa tttgcggtac tgcgccttca ttctttactg ctagaaatgt tttcattgtc
     3361 gtcattcgcc tcacctcctt tcgatacttg ttgattgttt ttagttataa atatttcatc
     3421 tgccatcgcc ttatcagagc gatcatttcc aacgcgttct cttccttcgt tgattgtaaa
     3481 tactccattt ctaatgccta catcaatagc gtcaaccaaa tctttgaagc tagtaatctt
     3541 gatcatagtt gtatccacac gtacaaaatt ccctgacaag tattcttcta cttcatagag
     3601 actagcgtta aacgcatcct gaataagttc agcaatcggt atgatttcga acattaaaaa
     3661 agcgtccact tgatccgata acccactcat gtctcccttt agtaggtttt tcggaacgtg
     3721 aaacgctgct gctgtcatct caaagatgtc gtctattaag ttttttatat ctcttgaatt
     3781 gctttggaag tttccgctga aatcttctaa tgtgtactca ttttgtaatt gaaataccgc
     3841 acctgcatta tcagcttcca taaaagcctt aaattgtgat gtcatcattt tattgatttg
     3901 atcttgtgtt gtattgtctt gcggtcggaa taaattccct ttcagtacgt atctacgagc
     3961 gttagagcgc ttgtaaacat tcatggcact agaaatgagt ttcccatacg cttgataata
     4021 cgcatcgact agttgcctaa tttgttgatc tgcgtatttt atatagataa catcactttc
     4081 tagaaattct ctatcaagga ctatgttgtt aatttgcact tgagaaaaca catcatcttt
     4141 caatgcatat tctgtgacat cccaactatc cgcaataaat atttcgctag aattattaga
     4201 cggagaaacg atcaatactt cattgtagaa tattaatctc ctgatcagtt tttttctaaa
     4261 ttctgttgca ttatttttct tattaggagc tacattcagc ctatagtaaa gatcattctt
     4321 tttatttttt ccatcttcat atgacttgaa ttccgcttta ctcatcgcat ttgcaatcaa
     4381 atcaatacaa gtttcaatcg caaattttcg atacacaaaa tcaacttgca atttacaaaa
     4441 gtattcttct aaaggaaccg ttgctttttt tgtgaagtat cctaccgcct tttgaaaaat
     4501 ccccactttc tcacctcctt tcaagttaga atactagagg agtaaatcca gttcctgtat
     4561 tttctactga gctatttgtg actgttacag gagcagaatc ataaatatca tctaaaaaat
     4621 tcaaaccatg aaggaatgaa aaaaagccat ccgtttttct agtttcaggt tctatttttt
     4681 catagcgtat atttccatta gaaatatgct cttcatatac attcatgcaa taccaacgca
     4741 taatcgcatc gtcaccaaaa aataaacgtt gattaataaa aaggtcatca accagatctt
     4801 ttaacatacc atgtgtaaca gatccgcttc gaacaatttc cacagtaaaa cctgcttctt
     4861 ctaaagcggg cttcaatatt tttgcacggt acatatccat agcgattttt ttaatataat
     4921 atttattact catttcaaga aaccaaccta caatataatc agcttctata ttttttccat
     4981 gaacgatctg tgattttcct tgatctatag aaatatctat aacctctcgt ttgatgtttt
     5041 gtaatcgaag ggctgattcg tggataaaag tatgttgtgt aaaataaaca tctttatcgt
     5101 attttcctag caacccaacg ctggcaaaat ctcgtctatc agcaaaatcg actgttccta
     5161 tcacttcatc cattttttca ggaaattctt tttctttcgt atgcagaaca tcatcatatg
     5221 aagcaacagc aaatcgtgta tcttccatag gtctgttcat tcgtttggtc atgaacgtaa
     5281 gtcttaaacc agcattacgt tgcatttgag agtattcttg aaacattttc cgttttaaat
     5341 ctgcattgta attaatagtt ggacaagctt tttcccacat gtcgggatca tcaacttcat
     5401 tatcgttatc caagcgacaa ataaatggaa acaaactaga aaattctgct ccatccttgt
     5461 caattccaag ttctccagaa agaatcattt ttgattcttc tataatgtca tcaagcggac
     5521 caccacgaac atgaccatta gttgtatcat aaaattctct ataatctcga attttaccac
     5581 caccagaagt agccacattt atcattgaat aatcttcatt ttcgtgaatt tcatcaaagc
     5641 ggtttgcacc tggtcgcttc ccatcttttg ttctagcatt tgccgtgtta taacgaagtt
     5701 tgctgtttgt agcgatattt tgaataactt ccttcgtagc tttaaatact tttttatcta
     5761 aatcaggatg atctttaatt actttaaata catcatcaaa actagtcttt gcttggcttt
     5821 cattattggc atagatatca atatcataat ttttaatacc gtgtttagcg gttagtagaa
     5881 agaagttgtt ccaagaagca aaaccagttt taccattacc acgtcccatt aatgaaagat
     5941 atctattgaa cactagtgtt ttatcttttt tccatcgaac accataaata aaacattgta
     6001 gaaatttttc ccacggaatt aattcgaatg gaaagtattg tgctggtata ttgattgaat
     6061 cctctaccat ctgcttatcg aagtaaatat cttctctagt aaagactctt tcttctagat
     6121 aattttttag caataattgc tctttgcata ccttgatagt gccttcttct atagctttga
     6181 accaattttc aatatgctta taactcagga attgattcat ttgcttcacc taccaattca
     6241 ggagtaatgg caagtttatc caacatcaat cccatttgtt tgttgacaga aacaagcaac
     6301 gctactgatt cattcttttt accattctcc agtctaatgc cgttctcgga tatatcttct
     6361 tccagtgata tcgccgtttc ccataaactg atataacgat caacattatc taagaatggc
     6421 tcaatatttg ttttctgact ttccaattgg cttattaaag agcggcgtaa tttttctctg
     6481 tagcgatttt gagacaattc gtttttaaac attttagccc tcctttcatg ataaagttcg
     6541 aaaaaatctc ttttcctgac agccccctcc gtttcatcac ccccaaaaaa tttgcgattt
     6601 attttaaggg ggggttatct caccatcgga atgaaagctt cagcgaagtc aatgtaataa
     6661 ttaatctctt caatactata tccaaaaaca ttttttattc tttcgacgtt attatcttta
     6721 ttcaacgctt ctcttacttg attcactttg tatttactac aacagttatc tgataacaga
     6781 tccctaatac ctacatagcg aacgtatatc aaacgtttaa ttaatccctg agtataagat
     6841 gaatactctt caatcttttc tgtatcatac tctctgccat tatcattgat gatcatgcac
     6901 ttaccacctt tcacttgcat cgaagttagc aaagctttct atcttcttct cttgtttatc
     6961 taatgctgta agatatctgc catgaacttc attatgatgt tcaacacata aacaaataag
     7021 attatctaaa tctaaagcta agtcaggtct atccttgact tcctttatat gatgaacgtt
     7081 ctctactcta tgatacttac ctagtcttct acactcttgg cattcatagt gatctcgttt
     7141 catcgctttc tctctaagcc tgcgccattt aggagactga tagaacttaa ccaaacgatc
     7201 ttctcttgtc aactgtaata gccatctata gaattcctcg gtcatgttcc gtctcctttc
     7261 gcaatcttat ttaatgctta gctattcttt tgccatacaa tggaataact tcattgtttt
     7321 cctttcgttt atatgtatcg ctctttattg gtcttctata ctcatatact ttttcgccat
     7381 tacctttatg aacagtgatt acttcatact tctgttctaa gtattgtggt ctatacattg
     7441 ttgtttcctc ctttgtgcaa aaataaaaag accactcaaa gagtgatcta atatgtaata
     7501 gtaacttgca cgatgcacaa aacgcgtacg aaattgcgca cccctatatt tttaaaccgc
     7561 cgatgcctcg gttgcttaaa gtcgctggag tgggattgca ccactcacga gaacttacca
     7621 ggctctcacg aggctactcg ccatttaccg ctgcgtcttc tacttccgcc acagtgaccg
     7681 aagcttggtg gtgtacagat agcacactta ctacattgcc gtacgtagca ccgtatagct
     7741 tcttaacgat cttttttcgg tagtcgtaac cgtcataggc atttaatgtc gctggcaagg
     7801 aatcgaacct tgcatggttg ccgaagcatt gacctagcac acatgcttag cgtctaccct
     7861 ttccgccaca gtgacactat aaaattattc ttggctgcta ctatttttta ttttgcccat
     7921 ttttaaatcc aatcatatag acattaagac agagcgcaaa aattgaaatt attaatggaa
     7981 tcatttctct tcacccacct ttagttatcg tgtgaataat taaaaaacaa tagacagcaa
     8041 caaaataaca ttgctttgat aatttggtat aaaccactat aaatttcttt tcttgcaatt
     8101 atttttaata tatgctagat tatcaaccga tatagtcact gcctgtacta gcggaaacta
     8161 gtgcaggttt tttgttctat ttactcaaaa gttattacga taaattaata ttgtgaaaat
     8221 aaatactaag cgtataattt tatttatcag cgagtggtcc gctgaaatat aaaacaagga
     8281 tgtgcaagac aacatgtata aaccctacat gattagttat gatcttaata acccagggca
     8341 aaagtatgat aaagtatttg aaataataaa agaatttggg gcatatataa agctacaaaa
     8401 atctttttgg ttagttaaaa ctaatttgaa tccaaatcaa atgtgtgaaa aattaaatac
     8461 agtacttgat aataacgatt ccttattcat ctgtgaactg caaaaaaatt atcaaggtag
     8521 agctacagag gaaaattgga aattcattaa cgaacatatt ttctcttagt aaggattaga
     8581 tattttttct ctgtttaaac aattgcaaat accgtcaata tctgaattag aaacacttac
     8641 cttttgctcc ttgctactac caatagcttg gagcaattct tgtatttctt ctggagtgcc
     8701 ttctactgat aatttcattt ttcttccctc caatacataa attaatagac agcagcagtt
     8761 gaaagacgac aagaacatgt ttagttttgt gagtgctgct gtctaatcaa aataaacagc
     8821 aaccgatgaa gaatttagga ggagttgaat tcacatcctt ttcttcataa ttagttgctg
     8881 tctatctaag aagaagttta aaacgatgag ggagattgcc tcccttcgtt tattttgtcg
     8941 atcctgtttc ctaatctttc gacactacca taataacact ggtaaatgtc taaaaaccgc
     9001 catcattccg ccaaaaaacc gccaaattat ttataagcaa ttatttttcc gtgtttatat
     9061 gcttctgcaa actctattag agcttccgac ttcatccgtt gtatacttct ttctgaataa
     9121 cccacttcac ggctaattct gtagtttgag aagctatctg gcacacagaa gctgtagtag
     9181 agtatctgac gactaatcag actaagagcc atcaaagccg ctagaatcgc atctctctcc
     9241 gtttctatat ccatcatctg aataatcgcg tcttctgcct tattgccatg cttcggtgcc
     9301 ttcggcatat ccgttataat cggcgactta atatctatca aagagcgacc tgccatccgc
     9361 tccaaacgcc gaaagttctt cagcacatct ctcgcattac atcttgtctg tttgaaatct
     9421 acctctcgta acaattgcat caagtcaaac cgctccttta tgtgatataa taaatgtgtt
     9481 ggatttattg aatcagtcgg agcgatccgg ctttttttat ttgctcttga ttacttccat
     9541 gtcaactaat ctcgctacag ctaaataatg cttgtgtttt gcagtcgaac gatcacactt
     9601 cattgtattt tcaatacgaa tgattgctga gtttgacagt atctttttaa cgtatcctct
     9661 gaacggataa acgaactctt ctgcttcaca gcggaccatg tcaccgactt tgaattttga
     9721 tttcttgcgt gttttagggt tctttgtcgg catatctagc attaaaccgc cgataccgtg
     9781 actgctagcg taaaatccgt cttttagttt catctttcta ccacctcttc cactgttcca
     9841 ttcaccaaca atgctgcagc atccgctgtt gctttatcag tgaaaagcaa tcgatgagat
     9901 ggattgcatt caaaactata atttactcca tcttttcccc ataaagcaca tttagtaaga
     9961 tagtttccat cggcattctt tattgcccat aacggctctt tctcaacctc gtagccgttg
    10021 tataggctca atagtgtttc atatgattgt cttgttgccc aagcgagtaa ttcttctcct
    10081 tgctcttgcg tcactatgcc atctcgtaca aaccaatcac agaaataata tgaatcacca
    10141 tctttagatt tgattaaata cgctattttt tcagcttttg taaaagggtc cgtagatttc
    10201 tcaagccaac cggccacgag ttgtggaata actggtttct gcggttcgtc tatttttttc
    10261 attaacgtaa tacctgaatt tatactctcg ttatagcaag cagctattgc atcatttcct
    10321 atcgctttaa tactttctaa ctttttgatt gcttcctgtt tattcatcgc tgttcctcca
    10381 ataactcgct attctcgtat atatttccgg tgacttcgta cgtgtattct tcaaacagct
    10441 ctgtgttaaa aatttgatac tctaagtctt catcaacagt ggcacaaact aatccggaat
    10501 aatctattga atttttgaca atacagactt tattatctaa gtaatcgaag ccatttcgca
    10561 cgctgactaa tactatatcc ccttcaaata tatccacacc attcttatct ttcattcctg
    10621 tggattgcat gaggtcgatg tttcgcacgt gatatgattt caattcggta ggatttatcc
    10681 aatattcaat aaagtttgtt ttgccgtttt tagtaaaatg caagacggct acatctctca
    10741 ttacgttctt tcgtttatcc cacgctctaa acttcggtat cattcgctgt cctcctcgta
    10801 tttttcaatc aattccatta cttttttcac tatttcaaac tcaaccgctt ttgattcttc
    10861 gaaatcatga acaatttctg gaaacagtac atcatcaact acccacaaaa taatctggcg
    10921 ctttccacca aaatttatga ttagatggtc cgattccaca gatagtgttg ctcctgattc
    10981 gatatcatat aaatccatgc tgaattgaac gagttttttt atcatttgct gtcctcctca
    11041 aatactcttc taatatttct ttatacttct ctacaaattt gaaacgatct tgatgaagtt
    11101 tcttgctcca atttgtttgc cgatccagct cacgcatctg atcgaaccct ttttgaattt
    11161 cgttgtaata aaattcaatg tttgctgctg ctttccaatg cctgctactt cgcactcctg
    11221 ctcctgtttc agccatttct aacttaacta attcagctcg ttcttttgat tttttatctt
    11281 tctgaatctt catcatgatt ttcttgagga tgatgtcact gtattgtgta atgagatcca
    11341 ttatctctcc tccacatacc taaactgtcg tccttttgaa tcaatccata agctcctagc
    11401 tctatcccag ataatgtttt tgcttaatcc agtaatttca gataactgtt cagcagtacc
    11461 tgttactaaa attcggtcat catgccagat tgcaattttt ctcggcgttc tccgtttggg
    11521 cttttcagtc cacattgatt taccgagctt ttggacttct gcaactattt ctttgtcttc
    11581 ttgccaagat tctgacttgg ttaattcagc aattcttttc attgctgctt tcttatccac
    11641 gctcattcct ccaatcgatg gatttccctt cttaaattct ctatgtgcaa atcgattgcc
    11701 tttctcgccg tttcattgac catcactgcc tttgttcgtt ccagatcgtc aatctcacgc
    11761 tgaaggcttc gaatacgcat ttgaatcact tcttctgttg tcatgatgat tcctccacgt
    11821 atctaaacgt tctcttctta gcgtctgtgt agccacacct tgctcttttc ctcacgattt
    11881 tctcgtgcaa gcctgtgaga cttgctaact gttcagctgt ccctatcact agaagtttgt
    11941 caccgtgcca aattgcgatt tttcgtggtc ttggcttgtt gctcttgtct gcccacatcg
    12001 ctcttccaag cctcatcact tctgaagcag cttctttgtc attttgccaa tcttctgaat
    12061 aagtcaattc gataattcgc tgcattgccg ctttcttatc catcccgaca ttctcctttc
    12121 agtaatttga gtacttgatc aagtgcgctc tcacgtccgc catggaacgt gttgagccac
    12181 ttgtcttcgt acgatacact ttgtcttaaa gcttcttgat gcattagttc aatctgtgct
    12241 gtaaatgtct ttaggtccat ctgattacac ctgctcaagt tcattaagat gtttttgcaa
    12301 gcctttaaca caatcaacaa atagtaattt ggtatatgct aaatttctta attgtgttgt
    12361 atcgatataa agtgcgaaat agtatctgag cttactccaa ctcgaacgat cattcttaat
    12421 tccttcaatt ccagcttctt cgagttgatc atatacgtct ctcaggacgt ctaattcctc
    12481 gcccttttta taattcgcta tttcattaat tagttctaga taatcgatct tcatttttcc
    12541 acctcttaga atggtgcttt tgattgtcta ttagctcgtt ctagcgcttt tttcttgaga
    12601 taggcttctt ggtcgattgc ccattcagga agcttctctc gtcttcctgt gcgcttgtat
    12661 ccactgcttg cgttcttagg ttcacttttt tctttccttg cccaacttcg aatagttgcc
    12721 aaatagtttt tataagtctt accagatgat tcacaatact ctgacagtcg ttcgattcgc
    12781 tcttggtaat cattagggaa ttctgttttg agtttctcca tctgctcatc tgacaaaaga
    12841 acatttttat actctccgta tttatgacgg acgggcttag ccttcgattt tttcgaaggc
    12901 ggtaactctc ttatatattc ttttgtatta ttaaatgtat tattaataga tgtattatta
    12961 tctttgactt tttcgtcaat aggggtattg cgtttttcgt caatagggta ttgattaatt
    13021 cgtaggtacc tattgattat ttgattggta ccctctttgt aaatgatttc ccgattcaag
    13081 tatccaaact taatcaaatc acttacccat cgcgatatgg tctctttatt cacaccatat
    13141 aaatctgcaa agtactcatt gcctgcccaa caaaagcctc tttcattaca caaggccgtt
    13201 atctctccgt ataacaactt agtatttggt ttaagtcttt tgtcgtacct tacgttggct
    13261 ggtataatcg cataataact tcgatgttct gtcattttta ccctccaata tttaactttt
    13321 tgattgtttt ctggtttaat ttgatccctt tgatttgata tttatttttg aaattaatca
    13381 cacctatttt gtgcttctct gtgtgatgga ttctgcagag tgctgcaaat gtgtactctg
    13441 catgatcaac ttctttgcgc tttcgtcttc ctagcgcttt gtcaaagtga tcgatgtcag
    13501 ctcctgtttt gccacagatg cagcagactc tttttgtgat gcatttgtag aagtaatatt
    13561 cttgattcgc aggtaaaatc tcatagcctt ctttgaaagg aatatgatgt tcaaagatga
    13621 aatctaagat gatatttgct aagacattgg catcactcac agttgtattc gattcgtctt
    13681 tcaggcttat tttgcgccct gtgacacctt caaaacgaaa gtagaagaat tccttccaga
    13741 agtccgttgg catgcctgta tcgataaaaa tatcgcctat cagcgcatag atgaagtttc
    13801 gttgctgtac ggtgaaacgt ctaggatcaa taaaacgaat ttcaatgact cgatcaccat
    13861 cgtagccgtc atacatcgtc tttagtcgat caatgttcac ttcctcattg atggttgcgc
    13921 ttatgtcttt tcctttgaac tttttcagaa ccgctgaata tgaatcgatt aatggtttaa
    13981 acactcatat cacttcttat ctaattcttt tctcttagct gctattgctc gctccatcaa
    14041 ggcacattgc tcatagctta actgttcaat agtttcaacg ttatcagcta agagccctaa
    14101 tttatctgtc tgctcattaa catattcgat taaggttttg gtcatatctt tacccatctg
    14161 ctcattgaaa gcttctagaa tcgtctctag catgtttaat ttctttgtat caattctagg
    14221 tggtgttgga atatcttccc cttgaaatac atataatccc agtccgtgta gagccaatgc
    14281 tttcacaaag catcgcttca atgagttatt gatttgcatt gcatttggtt taacaactgg
    14341 ttggtttcga taatctaaaa caggaaataa ttcggtttcc gtgtgtcctt taaccgttac
    14401 tgagacagat acataagtcc cagtttcatc cataagaaaa ggtttatatt cctcaacaag
    14461 aaagtcttga tgagttccag aaacaaccct gtagtgtttg tactcattaa tagttaccgt
    14521 tgcctgtgga tcattctttt tcataatctc ccacgcgtga gcccaagata aataatcaaa
    14581 atttcctttt ttcttgagaa ttttatttaa cttacgacta aaaagttttt caaagttcgt
    14641 tgtccctttg atttcactca tcaaattctg cctccatttc agcaatgtat ttcttacctg
    14701 gtccgtaata agagatatca atcaagttat ctctgtcgta ctcttctagc gcatcaatca
    14761 agccatcttc gatgacgtaa atatattcag gtttattcga atgcttcgat agatggataa
    14821 gataaacatg atcccaaata gttacatggt tgcctaaatc atcttgatcc caagctagtt
    14881 cttcattcgt caaaagattt cgtctgattt ttcgaccact tgtttcctca attttcggct
    14941 tgccccaatc aggatcaatc aaatattgat ctagagtgga aagttctttt tccatatgct
    15001 aaaatctcct tatgatgtgt tttctttgtg actctttgct tgccggcgga gtcacttttt
    15061 tatttgttgc catgcttttt gcttgtcaat atgttgttgg cttaggatga ttggtttata
    15121 gtatttccac cagcaattag caattgccgt ccctattctt agcgcttcag ctctattcat
    15181 tgtcatcacc aaaaagtctc tgttgtctgt tcagttgatc aatttccata cggatcgcag
    15241 tttctggcaa ccacatttca ataaatgaaa cagcatcatc gaatctctta cgaggtaact
    15301 cgccatatct tgggattgaa aaggtacgtt taaattcaga ccaaaatttt gagaatactt
    15361 ttttgctgat ttcttcataa gctcggcttt cttttccccc tagaactccc ataactttca
    15421 tatttccttt ttgcttaatt tcaaactctt gttgtccgct aattcgcata gtatctttaa
    15481 gcatggaaac atctttttta acatctttca tttcttctaa ttgatagatc atcatatctt
    15541 caattgtttg aggaacagta ttcttgcgaa taacatcttc catttcgttg aatgcttcaa
    15601 tgtatttttg tttgaagtag atagctttct ttcctgtaaa ccccatagcc agcaagaaaa
    15661 atccatctct actaatgaag aaaactcgtc gatttctgcc atatgaatct ggttcattac
    15721 cttctacaaa catctgtcca aaattggaca catctttttt tagtgcatca atatctctta
    15781 aaacatgttg atgttttttc tcgaagcttt ctgccacttg taagctcgta gtcacagctt
    15841 ctttattttt caaaattact aattcttgca ttatttcttc tctccttttt ggtataattt
    15901 agttaaaaac tggtggtgtt tattttgaat ttttttcact taacaatttt tcaatggatg
    15961 tcaataataa gttttatatt gagtacgttt ttaacaggga taaaaataaa aggaatgcgc
    16021 cctcaactag aagttgaact taatgcttct tacttcgctg cagatatgat ttatacaaaa
    16081 gtaatcattt ctaactattc aacagaacca gctatgttag ttaatttaga actttcatcc
    16141 gctaacttaa accgcagatg gtcagctact ccatttaaga aattaattgc taaaggagga
    16201 tcagtcgatg ataatagaat atactctgaa gctgtaccgc taaatattcc tcctaaaagt
    16261 gctatatctt gttaccttgc atttgaagta gggaaaatta attttaataa actccttaat
    16321 caccaaacaa aaatgatatt tactctaaat agaacccaaa tccacaaaat tgttgatatc
    16381 aaaaatacaa acttcccaat agagaaatta gtgaaagaat taaactgagt aatagtaata
    16441 tacattctag tttctctttt ctattcattt ttagtcagcc ccctcggttg gctttttcgc
    16501 tctgtactca gcttcagctt catcaagccc cataaaaatc caaaccatgt aaacaatcgt
    16561 tcctatcaac gcttgcctgc ttccccaaag ttctaaagcg tagatgatta gcggtgcgct
    16621 gaacactagc gctctattaa attttcccat ccgcttgcct ccttaagatt tccgaaaaat
    16681 ttgtttctaa aaattcaagt gttttacttc ttaaaaatag atatgtgtct cttccttcaa
    16741 ctggataata gacaaatcca tttttgtttt tctcgatatc gataatattt ctatatcttg
    16801 ggttctttaa aactctagaa gtaaaccaat catattttct gttaatccgt tctagcactt
    16861 ctggcaacgt catccatcta ccagtatcat cagctttttt taactcctca taatccactt
    16921 gggagataat tacatagcct tctggaattg ggatttttgc ttctagatat tgcattagct
    16981 gttcctccct ttacaattcg tacatagtga taatcgaatc tataattctg tttgcttctg
    17041 cagaagtctt tttaccgttt aaaattaaag ataagtagct tttgctaatt ccaaatcttt
    17101 cagcaagcat ggtgtaagtt aagaactttg aactttcgac atattctttg attttttctc
    17161 tatctcgttg agtgatttct gcaatgtcag tcatactaaa actcctttct aaccaatttc
    17221 ctctaaatcc atttgagggt aatatccttc tttttttagt aattcgtaga taaatagacg
    17281 ccctttctgt gtccatttgg tattcattac aattttagtg ccaccatcgg ctttcgggat
    17341 ctcagttgta tgagattttg tgtatccttg gttcatgtgt tttttgcaca ataaccattg
    17401 gttaccgact tttttctgaa tacctagttt atgaagtaat ttattcatct gttgtggaga
    17461 catcccataa tctgctgcaa tctgactaat tgttactgaa tctgtagaag ataatatgct
    17521 atctaaatag gagattttag gttcgtactc tgcaatcttt tgttctgcga ttaatcttcc
    17581 agttctttct tcttttagtt gagttgctaa ttgaatgatt gtatctggat taagcaaagc
    17641 ttcttctact ttttctggag ttagataacc tccatgtttt ctaattgctg gcaacacttc
    17701 acttgttacc caacgtttaa attttttggc agagggaagt tttgatttta agattaaact
    17761 gtacaaacca gattcgttga tgattgtcat ctctcttgat tgacctgagg tcgtgattcg
    17821 cgaccccatc ttatcttcca aatctacatg ccgcgacaaa gcatctttag tgtttgaata
    17881 acccaaaaca ctcgcaacgt ctttgcctac aaaatatggt tcatcattta ctaaaattgt
    17941 ccgaacttcg ttttgttcga aattaaaaat ttgcggtgtg ttcatatttc tcattccttt
    18001 cttttatatt cgtaaacaaa tttaacaact tttttctaaa ttcaattgac agtatctgag
    18061 taatactcta tactaagagc ataattaaat aagacataaa aacattgatt ttaaagcttt
    18121 cttggcggtc tgcatttaca tatcaatagt gttttttgtt gtctttttag ttgttaaatc
    18181 tgtttacaag atatagtata gctcattact cagaattagt caaattattt ctgcgtattt
    18241 ttctaaactt tttttgtaaa catttagaaa ggttgtttta tcgatgaata cctatgaaat
    18301 aataaaagac ttggcgaaga ggaaaaagat atctatacgg cagttagaaa ttaattttgg
    18361 atattctaac ggttatctag caaagtggaa aaataatact cctaattcag atgaattaag
    18421 tcggattgct gactatttcg atgtctctgt agactacctt ctaggtagag aagaaagaga
    18481 aacccctaaa catgtggatt tatcagaaga cgatactgtt ttttcttttg atggaaaaga
    18541 aatatctaag gagacaatgc gtaaagcgat tgcaattgct aaagctttag aggaaaatga
    18601 atagttggag tgatggtttg tatgtatttg aaattgaaag aaatgttgag cgaatacaat
    18661 ttgaaagtag tttatatgga gatgaaagag cctggtttct actatccaaa acccagaatc
    18721 atttttttga atgaaaatct atacggtgaa actgcagaag cctttcattt atctcatgaa
    18781 cttgcacatt tcagcgcctc tcattttgaa ttttcagttt tgtacgatac ttctacaact
    18841 tttcactcaa aatttgaaac agaagcagat aaaatagcta ttctaatttt attaaatatc
    18901 tatattgaga acgaactgac tgatgaatct cagtttaatc tcgaaaaatt tatggagtat
    18961 tattctattc agaataagct cagatacact tgttatgctg tctgccaatg ctattttaag
    19021 aaaaaatatt cttacgcaag gcaatatgta tgaatacatc aagaattatt aaaaaaaccg
    19081 atttcagcaa aacattaaaa aagcccgtgc tgcaacacgg gctctttcct catatatgag
    19141 cttctacaaa aaaaatcata tcacagaaat gaggaagaaa aaatggaaaa agagatttta
    19201 ataaaatctc caggaaaaac cttgattaaa gtaactgatg attctatttc aattattaga
    19261 aaaggattta taaatttagt taatcaaggt attaaaggag aaaaaactat cccctttaaa
    19321 aatatatcag ccgtacaatt aaaaaaacca ggtatgagta atggttatat acaatttacc
    19381 ttgctaggcg gaaacgaaag tcgcggaggt atattagcgg ccacgaaaga tgaaaacacc
    19441 gttatgttta caaaaaaata ttggaatgaa atggaaaatt taaaaaaata tatagagaag
    19501 caacaatcta tattagataa taattccaaa gaaacacaaa tttcaagtgc agatgaaatt
    19561 aaaaagtata aagagttgtt agatgaaggg attattaatc aagaagaatt cgattttaag
    19621 aaaaaagaat tgcttggttt ataatattca atatcaaaag agaaagagga tctattaatg
    19681 gacggtatat ttggttttac tggtattgtt ttatttatta taggtttaat catgttaatt
    19741 gtaagattta tcaaaaaaac aaataagaaa acacctatga ttctccttat tatcgggatt
    19801 atttttacta ccgtaggttt ctcgctttcc tcatcaaacg aggcaaatga caataacaaa
    19861 caggaaagct catcatctga tactcaaact actacatcta acagcagcaa taaaagccta
    19921 actcaatttg aagaattttt agagacaaat aatcatgact ggggtgcttt cttagatagt
    19981 tattattcta ttactccagc tactgagcaa aatacagctt ttactaaata tattagtggt
    20041 aaaacatata cttttgaagg aactgttatc gaatcaatga ctactagaat agccattata
    20101 gcagacaaag agtacgatga taaaagttgg aatgatattt ctgcaacacc taaagtttca
    20161 tatgtaattt ttgcgaagga cttaaacaat gctgatacct ttacaaaggg cgataaagta
    20221 acttttacag gagaaattag ttcaagagga tcgaacatag aaaaatccta tgctcaatgg
    20281 gatatgatta atagtaaagt aagtaaaaaa taaatgaacc acgccccact aagagttaga
    20341 ttgtgcacaa taataaaacc tatacaaaag agtgccatca ggagtcacaa tgcttgtaaa
    20401 ggaaatgaca aaataggagg taactatgga taatagcaat gaaaaactaa ccaaaaaaga
    20461 atatcttgaa ttaatagttg aaggcggtat tggagcaatt ccttatattg gtggcgttgt
    20521 gcagtcactt tattttgggt ctaaaaacga gaagcgtttc aaacgcatcg agaaattcta
    20581 caaaaattta aacgaatcat tagaaaacat tcgcgatcaa attccagaag atgttttcaa
    20641 cttagagaac aaggaccaac tcattggaat attcgaaggg ataaatgacg aggtagaaaa
    20701 atcaaaggca caaaataaaa tagatatgta taaaaatctt tataagaatt gtttacttcg
    20761 aattaacaac gcttcatggg ataacgagga gtattatctt cttgtactga atcaattaac
    20821 atcgattgag attcaattgt tagcatgctt aatgagtcgt ggaaacgaaa atttcactgg
    20881 aaatatcagc atgtctggct attcacaaga gctaatagat ggtagcttaa atagattatc
    20941 tgattttggt ctgttagaaa aaacgattag ctccatagta ttaggtgatg tcggcaaaca
    21001 aaatatgggc tacaaaatat ctcatttagg aattcaattt attaactcaa ctcttactta
    21061 agttttcttt ctaatatgac atctagacct atcagcaatg caacaataaa aaaccagaca
    21121 taccaataat caacaaataa tctaaactct ggaagttcag ttattctttt aaaaatcact
    21181 ttaatcataa taaacacctc tgattggatg atctaataca catccaatta taccattaaa
    21241 acgacaaata gcacatcctt gcgccggcaa gcaaaaagga tgtgcaaaaa ataataaaac
    21301 ccaaacaatg ggcttcttta tagttcctat tgtatcagag aaagagagct gattcaatta
    21361 tggcaagatt agtcaaacgt ggaaatagtt ggcaatacga aatttcatac aaaaaagatg
    21421 acggaaaata cacgaagata agaaaatcag gatttaagac aaaaggagaa gcaaaagatg
    21481 ccgccaacga attagaatat aacttgaaca aaggccttaa aggggatcgc aaaaatctat
    21541 tattatcaga ttactttgag gattggatgc aactttataa agaaggaaca gtatctccta
    21601 tcacttatag aaaatacgaa gatacgttaa tgaacataaa gaaatatatg ccagcggtat
    21661 tgatttctga tttagataga gtgggatatc aacgcttttt aaataagtat gctaaagacc
    21721 atgtaaaatc taccgttatt aagtttaata accatattag agcgtcgttg aaagatgccg
    21781 tagaagaagg attaattccg tttgatccaa ctagaaaagc agtaatcaaa ggaaaagatt
    21841 cattgaagcc aaaagaagat aaatatttag attatgatca atttaaatct ttaatgaaac
    21901 tcgtagaaga aaacctttct gcgcagtact cttctcctat gctcgtgtta gttgctggtg
    21961 ccactggaat gcgatttgct gaacttctag gattaacatg ggaagatatc gatttcgaag
    22021 atcaaatcat cactattaat aaaacatgga attataaatt aaatgaatgg ggaaaaacaa
    22081 aaaacgaaac ttcaaatagg aaaatttcca ttgataaaca tacgattgat ctcttaaaaa
    22141 agtttaaaat caatcaaaaa gaattattcg agaattttga aatcaaaaac cctcataatt
    22201 ttgttttttt caacttaaaa aatggattag tttcatcaaa cgccgtcagc aaatatttgc
    22261 gcaaaaaatt aaaagaatta gggattgaaa agcaatttac tttgcatgga ctaaggcata
    22321 cacatgcatc tattttactt tatcaaggag taaatatact tagcgtatcg aaacgtttag
    22381 gacatagcag tttagaaact acaatgtcta cttatcttca tattgttcga gagcttgaag
    22441 atcaggataa agaaaaaatc aatactgtgt tcgatagttt atataaaaat gataactagt
    22501 ttggcacatt tttggcacaa atacaaaaca aacccctgta ccgcaaagga tacaggggtt
    22561 atttatacgt tcctgactgg aattgaacca gcgacctatc gcttaggagg cgattgctct
    22621 atcctactga gctacaggaa caatttcaat aaatcaattg tagcttgttt gttttttact
    22681 tgcaagctta tctgctaaaa aaatcagctt ataagttagg caaaacgttt ccactagtcc
    22741 ttaacagatg ataaactaga ggtagaatga aataatgtag ggaggtcctt tccaatgaat
    22801 gataagccaa aagaaaaagt atacgcagaa cgcttagatt catctaaaaa aaatcgggaa
    22861 aagattacga aagaagcaat ccaagaccac aaaaaagaaa accctaaaac aaaagaaaac
    22921 aaaaaattca aatttacgaa aaaagaggat ttactttaac attttccttc aaatgccaaa
    22981 tgaaaaacaa aagggtggtg acaaaaatat tgtcatcacc cgttttttat caaaaatagt
    23041 cattaagaat tggctgttgc tgtggcaaga ttgagcttaa cgtttcttca tattcttttg
    23101 tcacctgtat tctttcttgg aataccaact cttccacttt acgataccct aataatagct
    23161 gcgtgatatg ttggatactt cctcgaacga ttgggagttt tgtttgttct acttttgaga
    23221 gatgattttt tccattttcg attgttactt ccacgattcc ttcattccat ttcgcataat
    23281 gatcttcttc tatttgtagg gcaaatgcag cttgtctctt tttaaat
//
LOCUS       DACDIH010000038        22033 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDIH010000038 DACDIH010000000
VERSION     DACDIH010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.038"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            156..1862
                     /locus_tag="IV281_001614"
     CDS             156..1862
                     /locus_tag="IV281_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP8780605.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="IV281_001615"
     CDS             complement(2020..2922)
                     /locus_tag="IV281_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8780606.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3623)
                     /locus_tag="IV281_001616"
     CDS             complement(2919..3623)
                     /locus_tag="IV281_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP8780607.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="IV281_001617"
     CDS             complement(3654..4295)
                     /locus_tag="IV281_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8780608.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="IV281_001618"
     CDS             complement(4333..4647)
                     /locus_tag="IV281_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780609.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /locus_tag="IV281_001619"
     CDS             complement(4635..5264)
                     /locus_tag="IV281_001619"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP8780610.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="IV281_001620"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="IV281_001620"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP8780611.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="IV281_001621"
     CDS             complement(5984..6916)
                     /locus_tag="IV281_001621"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP8780612.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="IV281_001622"
     CDS             complement(6913..7689)
                     /locus_tag="IV281_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP8780613.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="IV281_001623"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="IV281_001623"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP8780614.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="IV281_001624"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="IV281_001624"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP8780615.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="IV281_001625"
     CDS             complement(12014..13294)
                     /locus_tag="IV281_001625"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP8780616.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="IV281_001626"
     CDS             complement(13313..14239)
                     /locus_tag="IV281_001626"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP8780617.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="IV281_001627"
     CDS             complement(14252..15535)
                     /locus_tag="IV281_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP8780618.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="IV281_001628"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="IV281_001628"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP8780619.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="IV281_001629"
     CDS             complement(16323..17234)
                     /locus_tag="IV281_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8780620.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /locus_tag="IV281_001630"
     CDS             complement(17231..17695)
                     /locus_tag="IV281_001630"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP8780621.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="IV281_001631"
     CDS             complement(17700..18398)
                     /locus_tag="IV281_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8780622.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="IV281_001632"
     CDS             complement(18385..18771)
                     /locus_tag="IV281_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780623.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="IV281_001633"
     CDS             complement(18918..20588)
                     /locus_tag="IV281_001633"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP8780624.1"
                     /translation="MKNDLQISQETELEPIVEMASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="IV281_001634"
     CDS             20771..21004
                     /locus_tag="IV281_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP8780625.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="IV281_001635"
     CDS             complement(21103..21534)
                     /locus_tag="IV281_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP8780626.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="IV281_001636"
     CDS             21613..22026
                     /locus_tag="IV281_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP8780627.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
BASE COUNT         6235 a         4923 c         3889 g         6986 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccatttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaa
//
LOCUS       DACDIH010000039        22029 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDIH010000039 DACDIH010000000
VERSION     DACDIH010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.039"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..275
                     /locus_tag="IV281_001637"
     CDS             <1..275
                     /locus_tag="IV281_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780628.1"
                     /translation="KVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIHRFIRMQQ
                     LPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            334..774
                     /locus_tag="IV281_001638"
     CDS             334..774
                     /locus_tag="IV281_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780629.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            786..1454
                     /locus_tag="IV281_001639"
     CDS             786..1454
                     /locus_tag="IV281_001639"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8780630.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1518..2141
                     /locus_tag="IV281_001640"
     CDS             1518..2141
                     /locus_tag="IV281_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP8780631.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2197..2583
                     /locus_tag="IV281_001641"
     CDS             2197..2583
                     /locus_tag="IV281_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP8780632.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            2796..4187
                     /locus_tag="IV281_001642"
     CDS             2796..4187
                     /locus_tag="IV281_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP8780633.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            4798..5166
                     /locus_tag="IV281_001643"
     CDS             4798..5166
                     /locus_tag="IV281_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8780634.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5346..6287
                     /locus_tag="IV281_001644"
     CDS             5346..6287
                     /locus_tag="IV281_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP8780635.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6356..7087
                     /locus_tag="IV281_001645"
     CDS             6356..7087
                     /locus_tag="IV281_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP8780636.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7231..9411
                     /locus_tag="IV281_001646"
     CDS             7231..9411
                     /locus_tag="IV281_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP8780637.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9431..9913
                     /locus_tag="IV281_001647"
     CDS             9431..9913
                     /locus_tag="IV281_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP8780638.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            9929..10690
                     /locus_tag="IV281_001648"
     CDS             9929..10690
                     /locus_tag="IV281_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP8780639.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            10821..12155
                     /locus_tag="IV281_001649"
     CDS             10821..12155
                     /locus_tag="IV281_001649"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP8780640.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12462..12947
                     /locus_tag="IV281_001650"
     CDS             12462..12947
                     /locus_tag="IV281_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP8780641.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            12940..13623
                     /locus_tag="IV281_001651"
     CDS             12940..13623
                     /locus_tag="IV281_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP8780642.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            13749..16394
                     /gene="polA"
                     /locus_tag="IV281_001652"
     CDS             13749..16394
                     /gene="polA"
                     /locus_tag="IV281_001652"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP8780643.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16439..17275
                     /gene="mutM"
                     /locus_tag="IV281_001653"
     CDS             16439..17275
                     /gene="mutM"
                     /locus_tag="IV281_001653"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP8780644.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRQKI
                     AK"
     gene            17239..17868
                     /locus_tag="IV281_001654"
     CDS             17239..17868
                     /locus_tag="IV281_001654"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP8780645.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            17920..18252
                     /locus_tag="IV281_001655"
     CDS             17920..18252
                     /locus_tag="IV281_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8780646.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18564..19055
                     /gene="nrdR"
                     /locus_tag="IV281_001656"
     CDS             18564..19055
                     /gene="nrdR"
                     /locus_tag="IV281_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP8780647.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19078..20451
                     /locus_tag="IV281_001657"
     CDS             19078..20451
                     /locus_tag="IV281_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP8780648.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20451..21377
                     /gene="dnaI"
                     /locus_tag="IV281_001658"
     CDS             20451..21377
                     /gene="dnaI"
                     /locus_tag="IV281_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP8780649.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21506..21946
                     /locus_tag="IV281_001659"
     CDS             21506..21946
                     /locus_tag="IV281_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP8780650.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         7469 a         3811 c         4809 g         5940 t
ORIGIN      
        1 taaaagtgat cagtaaaaaa cggaatctga tcaaaacgac acagctgttg aaaaaatatc
       61 caggtctgct tcacacgtct attcaatttc ctcaattgat tacgatgatc catcgtttta
      121 tacgtatgca acagctacca tctaagaagc tgtttcttcc attgacggac acatttcgag
      181 tagtcaacag agaagataaa aagaaagtga ttgtgattga ctttaccaga aatcggaatg
      241 tccttcacca agttgcaatg gaaaaggcca attaaggctt tttctttttg gatttttttg
      301 tcataataaa tattagatga tggaggagat aatatgatgc aaacatggtt agaaaggcta
      361 atacgatttc agcagaacta tcacaaactt atgcagggaa gatatgcgaa atttgatcag
      421 ttgaatcgcg gtctattgat cgctgcgctt gtctttagtt tgctaagtcg ttggttgccc
      481 tatagaatcg gacaattgtt gttcatcgtc tttttcggat ggatgatatt tcgtttccta
      541 tcaaaaaaaa tctaccccag actaaatgag aatcaacgtt atttgaaata tcttgataga
      601 gtcaaagaaa aatatcatca gataaaaaac aaaaagcaag tcaaaatgga tcgaaaaagt
      661 tatacttttt ttgagtgccc gaattgtcat caaaagcagc gtgcacctaa aggaaaagga
      721 cgaatacgtg tgacatgcaa aacatgtggt ataaaattcg aaacgaatgt ttaagggaga
      781 aaagaatgaa attagtatta ttacgtcatg gcgaaagcga agcaaatttt gaaaattact
      841 ggacaggatg gctagatgtc gccttaacag aaaaaggaaa agaacaagca gcagttgccg
      901 gtcaaaaaat gcttgaagcc ggacttcaat tcgatgccgt ttatacctct gttttgaaac
      961 gtgcgattct taccggacaa gtcgcactag aagaaatggg tcagttatgg cttcctataa
     1021 taaaaacatg gcgtctaaat gaacggcatt atggggatct tgtgggtaaa aacaaagata
     1081 agatgaaaga aatatacgga aaagatcaag taaaaacatg gcgcagagga tatacggaag
     1141 ttccgccgtt aaccgcagaa aatagatttg accgcagata tgatcacttg gatccgcatt
     1201 tgatcccata cggagaaagt ctggaaatga cagtgaatcg gatcattccc ttatggcaag
     1261 atcatctagc accaaaacta aaagatcggc aagatttact agttgtgggt catggtaata
     1321 gcatccgagc actgacaaaa tatttagaag atgttcctga agatcaaatg gatacgatcg
     1381 atatcccaaa tgcacaacca atccagtata cattagcaga tgatttgaca attctggaca
     1441 aaaaaatact ttaaagaaca cctttatcta aaagtgagag atagaaaaaa gaagcaaagg
     1501 aatggaacaa aaggaacatg aaaaattata tatgggactt tgatggtaca ttgtttgata
     1561 cgtatcccgc aatggtagat ggtgcatggc aagcactaaa agattttggt atcagtatgg
     1621 acaaaaaaga aatctacttc aaaatgaaaa agtattctac tagttatttg atcaatgaat
     1681 caaacttgaa tgccagagag tttaatgaat tatttcaccg atatgaaaaa gaaagtacgg
     1741 aagtttcccg tccatttcca gaaacgaaac aagtactgga aatgctaaaa gacaacggcg
     1801 gtcgtcattt catcctgacc caccgtttga cagaatcaac atggggcctg ttaaaagaac
     1861 atcgattggc acatctgatt gaggaagtag tagggatcga tcaggacttt cctcgtaaac
     1921 ctgatccagc atctttaaat tatttgatcg atacttttca tttagaaaga acagacacga
     1981 tgatgatcgg cgatcgtcgt ttagatatcg aagcagggaa aaatgcagga gtcgctactt
     2041 gtctttacga catcgatcat tttctgggtg agatcccggc agattatgtg gtaggaaatc
     2101 tgaatgaaat ccttaccttg cagagcatca ttaaaaagta gtatcccctt tcaaaggaga
     2161 tatgttataa ttttcataaa ggagtgataa aattatatgg aaatcagaag acaaaaagaa
     2221 gttgtagaag catttcatta tgatatgaga ttgccgaacc aagaagtaga aacagatttg
     2281 aaagtaggct tctcgccttt aaaagaaacg atcgaaaact atccgaaaga acatagcatc
     2341 gtaggagcac gcttagagtt ccgtttagtg tttgatacat atgttttatc tggaagtgtg
     2401 agtcagatca atcatatcct caatcggaaa attgaaggtc aaaatgatgt cacacaagaa
     2461 gaagtggacg agctagtagc accgttattc gatatcgtac gccgaatgtc ttatgaggtg
     2521 acagagatcg ccttagatca gcctggggta aaattgaatt ttcaatcaga aaaacaagaa
     2581 tagacagtaa aaacatacct gcgatagatt aaagctatca caggtatgtt tttttattac
     2641 taattgtaag caaaaagaat gtcatttcga taaaaatcgt ctaaaatata aaagtcactg
     2701 aaaaaaattt tttcaggagg atggttgaag aatcattctc gggaaccttt acttccttcg
     2761 atggacttgt cattaataat atgaggagga gaaaaatgac aacactcatc acagttttag
     2821 tggttctagt ttttgcggca ttattatatg tgttctatcg tatgcaaaag cgccatacaa
     2881 aattttcaac acgcgtcttt acagcattag gagttggtat cgtctttggc ggtctgcttc
     2941 aattgatctt tggtgtagac gacaaagtcg ttactcaatc aatggaatgg atcgggattg
     3001 taggtcaagg atatgtatca ttcttgcaaa tgctagttat gcctttagtg tttatttcga
     3061 tcattggtgc attcacaaaa atgaaagttt ctgaaaagct agggaaaatt agtgcgactg
     3121 tattgacttc tttacttgga acaacagcta ttgcagcatt gatcggaatt ggttcaacaa
     3181 tgctgttcgg actccaagga gctaaattca cacaaggagc tgcagaaaca gcacgaatct
     3241 ctgaactggc aacacgtcag gaaacagttg aaaacttatc gattccacag cagattttat
     3301 cctttatccc taaaaacgtt tttgctgatt tagcaggtag tcgtgcaaca agtacgattg
     3361 gagtcgttat tttcgcggca tttgtcggaa tcgcttatct aggcgtgaaa agaaaagcgc
     3421 caaaagaagc agcattcttt tcagatttag tcgacagtct gtataaaatc attatgcgga
     3481 tcgtgacgct tgttttgcgt ttgacacctt atggagtatt tgctttgatg acaaacgttt
     3541 tggcaacaag tgattttgca gctatcgtca atttaggtaa atttgtcgtt gcttcttacg
     3601 tagcattgct tgctgttttg attgtccact cactgatttt gattggtgca aaagtcaatc
     3661 caatcaacta tttcaaaaaa gcctttccgg tattgagctt tgcgtttact tcacgttcaa
     3721 gtgctggagc tttgccattg aatatcgaga ctcagacaaa agctttagga gtagatgatg
     3781 caacagctaa tttctcagca agttttggtt tgtctattgg acaaaacggc tgtgctggcg
     3841 tttatccagc tatgttggca acgatcgtcg caccagcagc tggagtgaat gtctttagtt
     3901 tggaatttat tttgatgctt gtggcaattg taacaatcag ttcttttggt gtagctggag
     3961 tcggtggggg cgcaacattt gcttcactga tcgttttagg ggcaatgaac ttgccagtag
     4021 caatcgttgg gttagttatc tctgttgaac cattaatcga tatggcacgt acagcagtaa
     4081 acgtcagcgg tagcatggta gctggtatcg tgacaagtgc acgcatcaaa gatttagatc
     4141 gcgatgtcat caatgatgat tctaaagtga tcgatgtgaa tgcatagtca aaaagaggtt
     4201 gtgatagaag tggtcagctt taagaaataa gaagaagttt cctaaaaata gtttctcaaa
     4261 tttttgtgaa atttcggctt atttccgaga agctacttct gaaacaccgt gaataagagg
     4321 ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat
     4381 attttacgtt tggttgagct tattaccgca gatgcaagct tttgaacacc gtttaagaaa
     4441 gaggtcgtga gcctgccgtt tcactctgag tcacaaagag caatttcaaa aaatagcttc
     4501 tcatgtttgt tgaatattgt gtcttgtgac cgaggagttg tcaggagaac accgtttacc
     4561 agaggttgtg acttgttttt agtcacgacc tcttttttta tttattccat ataaatacac
     4621 attatttatt ccgaaaagtg aaagcgcaaa taacaaaaac aaccaactta tttattatta
     4681 aagaaaagcg tatttaagta ggacgattct gttgagagct ttcgtagatt ttcccatttt
     4741 cctatggtaa actgacaaag ttgagaatgt tagaacagat cgtttaggag gaaacagatg
     4801 tttacgaatg aaataaaaat catgctttat gtggacgatg tagaaaaaaa tgcaacgttt
     4861 tggagagcag ttggctttgc ggaaaaagat cgtcaagaaa tggatgggac gcttataatt
     4921 gaagtaggag ttgaggacag tcaaacctca tttctgttat atgatcgagc atttgttgag
     4981 tcccattcac ctggcacagc tactagtgcc ccatctttga tgttttacag taaagatatc
     5041 tttggactat ataaaaaaat gcagacgcaa tcagtcacat taggtgatat ggtacagctt
     5101 ggagaagaat atgtctttaa ctttgctgat cccgatggca actactttgc ggttacaggt
     5161 aaataataga aataaagact ttgtttttct gtgcgaaaca ggaaagcgaa gtctttttaa
     5221 ttcaaataat aatattatgt aaacaaaaaa ctttccatgc gtaattatac acaagcaaat
     5281 aaatcgtcaa atgcgaacgc cttctttata ctgtttagga acgaaaagaa ggaaggaaga
     5341 aatacatgga ttatcaagta ctgctttatt acaattatac aaaaatcgaa gaaccagagg
     5401 aatttgccaa aaatcattta gccttttgca aatctttgga tcttaaaggt cgtattctag
     5461 tagctgaaga aggaataaat ggtactgtgt ctggaaccgt tgaaaacacg actgcctata
     5521 tgaaagcaat gaaagctgat cctcgatttg ctgatacgat gttcaagatc gatccaagcg
     5581 aaggacatgc ctttaagaag atgtttgtaa gataccgtcc agagcttgta agtttaaagc
     5641 tagaagagga cattgatcca aaccaagtga caggtgccta tctttcaccg caagaattta
     5701 aagaagctat tttagatgaa cagacagtgg tgattgatgc acggaatgat tatgaatatg
     5761 acttgggaca ttttcgaggg gctgtacgcc cagatatacg cagttttcga gagcttccag
     5821 actggatccg cgagcacaaa gaagaattta tggaaaaacg ggtagtgacc tactgtactg
     5881 gtggcattcg ttgcgagaag ttttctggtt ggcttgtccg tgaaggattc aaagatgtcg
     5941 gacagctgca tggcggtatt gctacttatg gtaaggatcc tgaagtccaa ggagaattat
     6001 gggacggcaa gatgtatgtg tttgatgaac ggatcgctgt cgatatcaac caagtagata
     6061 aacaaatcgt cggtcgcgac tggtttgacg gaacaccatg tgaacgatat atcaattgta
     6121 gcaatcctga gtgcaatcgt cagattcttg cttccattga aaatgaagaa aaacatttag
     6181 gagcatgtag catagagtgt gcgaaacatc cgcgcaatcg ctatgttcaa gaccatggaa
     6241 tctctccaga agaagcagaa ctgcggttta cagctttaat taaatagaaa acaatagttt
     6301 tgattgtcga ccatttccaa tatgcgctat actagcgttg aggatggtga tccacatgaa
     6361 atcattgatt tatgctattg gggatgtcca tggcaaacat gagctttttg aaaaacttat
     6421 tcactattat gatccaaaga tccatcagct tgttttatta ggggatctca atgatcgagg
     6481 tcctcgtaca aaagaatgtt ttttattagg aaagcaatta gtcgaagaaa caggagccat
     6541 ttacctccgg ggaaaccatg aagaatattt tttgcaattt ctgcaatcac cggaggactg
     6601 gtatgcgcct tatatgagaa atggtggaaa agaaacgttt gaaagcctga ttcacccagg
     6661 ggcatcggag gaatactccc caactgaaat ctcgatgatg atccaatcga gatattcaga
     6721 actgattaaa tttttagtcc agcgcccctt gtattatgaa tgggaaaatt atctttttgt
     6781 ccatgccggt gtggatttga caaagaaaga ctggcgtaag acaagtctaa gagactttat
     6841 ttggataagg gaaccatttc atgaagggaa aaacaataca ggtaagacaa ttgtatttgg
     6901 acatacgatt acaccaatgc tccatggaga tatgcagaca acagacctct ggattcagga
     6961 tcataaaatc ggaatggacg gtggggctgt attcggaggg tcattgcatg gggttgtttt
     7021 tgacaaggaa ggtattcttc aagatattga atatcagaac aacgaaggac cttggcatgg
     7081 aatctgacta atggaaagaa tgaaggagct gtgactttgt tgcggctctt tttattttta
     7141 tagagaatat tgtttctttc agtagtttaa cccaggataa aaagtatgat agaataaaga
     7201 agcaattttt agcagaaaag aggcatttta atgacggaaa atttgaacca gacgatcatt
     7261 cagcttgtac aaaaagaact ttcagactat cgtcctaagc agttgactac tgtacttaat
     7321 ttgttaaatg aaggaaacac tgttcctttt atcgcgcgtt accgaaaaga aatgactgga
     7381 agtcttgatg aagtccagat tcgtgaaatc gaagagcggt atgcctatct tgaaaactta
     7441 gaaaaacgta aaactgaagt gattcggcta atcgatgaac aagggaaact gactcccgag
     7501 ttagaagcag aaatcaccca agccgtgaaa atgcaacaag tggaagattt gtatcgtcca
     7561 tacaaacaaa aacgacggac aaaagcaacg attgctaaag aaaaaggact tgaaccactc
     7621 gcgaagtggc tgatgcagct tacagatgga gaggtgcaat ctgaagcaga aaaatatatt
     7681 gacaaagaaa aagaagtctc ttctgccgaa gaagcgttgc acggtgctca tgagattatt
     7741 gctgaacaag tcagcgataa tgcaaaattt cggacttgga ttcggtctta tacgtataac
     7801 aaaggtatgt atgtcagcaa tgtcaaggat gagcaagcag atgaaaaagg cgtatatgaa
     7861 atgtattatg actttgcaga acccgtccat aaaatggttt ctcatcgaat attagctact
     7921 aatcgtggag aaaaagaaga tattctgaaa gtattctttc aagtagatga agcagcaatt
     7981 ttagcttact tggatcgcca aatagtcaaa aatcctgcct ctccttcttc ttcattcgtc
     8041 agagaagcct atcaggatag ttataaacgc tttattcagc cagcaatcga gcgagaactg
     8101 cgcaatgaat tgactgaaaa ggcagatgag caagcaatcg ctattttcgg cgaaaacctc
     8161 cgtaatcttt tgttgcaacc gccactaaaa ggtaaagttg tcctgggatt tgatccggct
     8221 tatcgtactg gatgtaaact agcagtcgta gatgcgacag ggaaagtttt agcaatcgaa
     8281 gttatttatc ctcataaacc tgcagcgcaa gcaaaaagag aagctgctgg accagctttt
     8341 atccaattaa ttaataaata tcaagtagat atggtagcta tcggaaatgg tacagccagt
     8401 cgggaatctg aactttttgt ggctgagcag ttgaaatccg ccgaccataa aacctattac
     8461 gctatcgtca acgaagcagg cgcttctgtt tactccgcta gcgaaattgc acgaaaagaa
     8521 tttccgcatc tacaagttga agaaagaagt gctgtttcta ttgcccgtcg cctgcaagat
     8581 cctttagctg aattagtcaa aatcgatcct aaagctgtcg gagtaggcca gtaccaacat
     8641 gacgtttctc aaaaacgttt ggctgaacaa ttggattttg ttgtggaaac agcggttaac
     8701 caagtaggtg tggatgtgaa tacagcaagc ccgcagctct tgcagcatat atcaggattg
     8761 aataaaacaa ctgcccaaaa cattgtcatt tatcgtgaag aaaacggaga attcaccgca
     8821 cgaactcaat tgaaaaaagt gccacgctta ggaccaaaag catatgaaca agcaatcgga
     8881 ttcttgcgag tccctggtgg gaaaaacatt ttggacaaca caggtatcca tcccgaaagt
     8941 tattctattg caaaagaaat attaacttca gtccaacttt cagaaaaaga gcttggtaca
     9001 gaagaagcag tagaaaaatt gacacgatta tccgtagaga aactggctga aagcctatca
     9061 gtcggagaag aaacgttagc cgatatttta gctgggctga cacaacctgg acgagatatg
     9121 cgtgatgaga tgccagcgcc cttattacgt acagatgtat tgagtatgga agacttgaaa
     9181 ccagggatgg aattgactgg aacggtccgt aatgtcattg acttcggcgc atttgttgat
     9241 atcggtgtga agcaagacgg acttgtccat atatcgaaac tcagcaaaaa attcgtcaag
     9301 catcctacag atgtcgtgtc agtaggagat atcgtaacag tttggataga acaagtcgat
     9361 acgaaaaaag gcagaatcag tttgacgatg ctttcccctt atgaagagta acaatgagga
     9421 gtaacaaaaa ttgacacaag aagaactgca gcagttagta gaagagatat cattaaaaag
     9481 cttccagctt cctttcaagc accaagcaat gttcaatcca cgcttacgta caactggcgg
     9541 gcgctatcat ttaaacgatc atcatttaga ttttaatgcg aagctatttg aagagtcaga
     9601 cgaagagacg attgcaggaa tcatcaaaca tgaactttgc cattatcatc ttcatctagc
     9661 tggaagaggg tatcagcata aagatatgga tttcaagtat cttttgaaaa aaacaggagg
     9721 gttgcgctat gcaccagcct tgacgaagaa acagataaag atggagtatt atcgatgcca
     9781 agattgttct gccacgatct gccgcaagag aagaatcgat gtacgcaaat atcgttgtgg
     9841 aagatgtcat ggagaaatca aatggattga gacaaaaaga gaagacttta atgaagcaca
     9901 aaaaatcaga tgaaaggata gaaggccttt ggaaaaaagt gagcagagga atctaaaaga
     9961 ctggaatcag ctattaaatt ttttagccgc acctgaagag ggaaattttg aggaactgac
    10021 ggcacctaca cttattttag ctggaaactg tcttcctatc ttagctgata aagcagcaca
    10081 aatgtattta aatggccaag tagatcaatt gtttttggtc ggaggggtgg ggcatgcgac
    10141 tagaatcctt tatgagaatt ttgaaaagca aggatttcat tttgaagatg gcatgagtga
    10201 aagtgaaatc tgtcgtcagt atctaaaaga agtctatgat ttgcccgata aggcttttct
    10261 gatagaatcg aagtctacaa attctggaga gaacgccact ttttcactgg agattcttca
    10321 ttccttagga gctgtaccag aaaaaatctt attgatgaat gatccgacac tgcaacgcag
    10381 gactcgagca acttttgaga aagtatggca aaatgaacaa actattttcg cgaatgctgt
    10441 gccatttgtg ccggaaatcc ttcacttttc tgaagagatc atctttacag caaaagaact
    10501 gaatcatcaa tggcctaagg aatatttcca tgcgctagta ttaggagaaa tggaacgtct
    10561 ccatgacgat gagaacggct atggtccgaa aggaaaagat tttattcctc acattgatat
    10621 atcagaggaa gtttggtctt cttacaccag aattaaagag tcaataaaaa cggatttttc
    10681 tcgtacttga ccattttagc aagcgaatcc tcctttccaa ataaatttgg ttgaaatttt
    10741 caatcgttta taagataatg aggagcgtgt tgaatgtaat agctaaaaga atggacgcta
    10801 gataagagaa gaggattcaa atggaaaatc aaaataaatt gtatcatttt gttggtatca
    10861 aaggttcagg aatgagctcc ttggctcttg ttttacatga acaaggtcta aatgtccaag
    10921 gatcagacat tgaaaaatat ttctttacac aaagagattt agaaaaagca aatatcacga
    10981 ttctgccatt taacgcagat aatgtaaaac cagggatgac gatcattgca ggaaatgcat
    11041 ttcctgattc acatgaagaa atccagcgag caaaagaatt aggtttggaa gtgattcgct
    11101 atcacgactt tattggtcat tttatccaaa attatacgag tatcgctgta acaggctctc
    11161 acggaaaaac aagtacgact ggactgctct ctcatgtatt gagtggtgtc cgtccaacaa
    11221 gttatctaat tggagatgga acaggacatg gtgatccgca agcggaattc ttttcatttg
    11281 aggcctgtga atatcgccgt catttccttg cctattcacc agactatgca atcatgacca
    11341 atatcgattt tgatcatccg gattattata caagtatcga cgatgtattt acagctttcc
    11401 aaacaatggc tggacaagtg aaaaaagcta tttttgcgta tggtgacgat gcttatttac
    11461 gcaaattaaa agcaaatgtg ccgatttatt attatggtgt cacagaaaac gatgatatcc
    11521 aagcacgaaa catcgaacgc acaacttctg gttctgcttt tgatgtctat catggggatg
    11581 aatttgttgg gcacttcact gtaccagcat tcggtaaaca caatatctta aatgctttag
    11641 gtgttattgc agtagcttat ttcgaaaaac tggatctgaa agaagttgca gaagaaatgc
    11701 tgactttccc aggagtaaaa cgtcgattca gtgaaaagat cgtagcagac atgaccgtag
    11761 tcgatgatta tgcgcatcac cctgcagaga tcaaagcaac gattgatggc gcacgccaaa
    11821 aatatccaga taaagaaata attgcagttt tccaacccca tacgtttacg cgtacgattg
    11881 cattgatgga tgaatttgca gaagcacttg atttagctga taaagtttac ttatgcgata
    11941 tttttggttc ggctcgggaa gaacaaggaa acgttaagat tgaagacctt ggagcgaaaa
    12001 tcaaaaaagg tggagaagtc atcaaagaaa ataatgtatc tccattgtta gattatcatg
    12061 atgcagtagt aatcttcatg ggtgctggag atgttcaaaa atttgaacaa gcgtatgaaa
    12121 aacttttaag cagcacgacg aaaaatgtac tgtaatacaa ttactgcagt gtccttataa
    12181 aaatagaaag agggggagtg gcaaaaatct tgccaagccc ccttttttgc aacttatgat
    12241 aaacggtgtt caaaagcttg catctgcggt aataagccca atcaaatcaa aaacatgaga
    12301 agctgttttt tcttggctgt tcttattatt tgatgcagga cagctttttc acgacctctg
    12361 taaactatag gaatctgtta aatcaaggga gcagaacttg ccttttacgc tacttttggt
    12421 acaataatgt atgattaaaa ataaactgga gggtttctat cttgaacata ggtaagccga
    12481 gaaaactagg aaaaacaata aatgaaatcg aggaaggcga ctcgttatcc ttaacagaat
    12541 ctattgaaga taaccagctt ttgctgtatt tgggattgac gaatgatgca aatcctttgt
    12601 atatccaaca tgactttgca caaaaaacag aatatcaaca gccaatcgtt ccatcagtga
    12661 tgctgatggg gatcatcaca agtgcaattt caaagcatct tcctggacca ggatcacatg
    12721 ttgtcaattt ttctgtcaat tttattgaac cggttttcca ttatgaaaca ctgacttttc
    12781 agtttgaggt cataaaagta gataagatga aggatgtggt gacgatttct gtagaagcgg
    12841 agaatacgaa cgaagatcgt gtgttggatg ctgtcgttat ggtccaaccg ccacaagttc
    12901 ctgaattgga aatagcaatc gaaagtgaag gtgaattgaa tgaataatcc aagtacatca
    12961 atgtcaggta tcaatcgttt ttatgcaaaa gtttatggat ttttaggtat gggacttgcc
    13021 cttagtgcag tcacttctta cttggctctt tgggtatttc ctttgcaatt agcaactttt
    13081 atcaataatt ttccactagg ctttgtgggt ctgtggatcg ttgaactgat tttagtaatg
    13141 gttctaggcg taaaagcaca aaagaatccg agcttagcaa tcggtggatt tattgcctac
    13201 tctcttttga atggcataat attagcggtt actcttgcat actacgatat tggaacagta
    13261 acgcaagctt tcttgacagc tactttgatg ttcatgggga tgtctgttgt cggagtaaca
    13321 acaaaaagag atcttagcgg ggttggaaga gcaggacagg cagctttaat cggagtgatc
    13381 attgcactag tcttgaacat cttcattttg agaagtagcg tagttgagct gttcatctcc
    13441 ctactgttag tcgtcatctt tgcagggatt acagcttatg ataaccaaag catccgtaat
    13501 atctatgtcc agacaaacgg aacagcacat agtggaattg ctgtattcat ggcattgaag
    13561 ctgtatttgg actttatcaa cttgttcttg gcgtttctga gaatctttgg acggaacaac
    13621 taaaaaagaa aaaaattcat cagttggggc tataccgaat tatttggtat ggtcccgctt
    13681 tttcttttcg cattccgcag gattctttgt tagaatgaat aagaacttta cagtaaggag
    13741 ttaagaagat gacaaaaaat aaattattac tcgttgatgg gaacagtgtg gcttttcgcg
    13801 cattctttgc gcttcacaat tctcttgaac gtttcaaaaa tagaaacggt cttcatacaa
    13861 atgcaatcta tgcattcaat acgatgtttg aaaatgttat gcaaaaagag caaccgactc
    13921 atgttctggt tgcgttcgat gcaggaaaaa cgacttttcg gacagaaatg tatgcggagt
    13981 acaaaggtgg aagatccaaa acacctggcg aattcaaaga acaaatgcca tatatacgtg
    14041 atttgttgac aggactaggg gtacagtatt acgaattgcc caattatgaa gcagatgata
    14101 ttatcggtac tttggcagaa aaagtagaca aagatcaatt tgatgtcgtc gtgctttcag
    14161 gtgatcggga tttgactcaa ttagctagta aagaagtaaa agtggatatc actgtaaaag
    14221 gtgtcagcga tatcgaaagc tatacaccag aacatgtagc ggaaaaatat gatgggttga
    14281 cgccaaaaca gattattgac atgaagggac tagccggaga tgcctctgac aatatccccg
    14341 gtgtgactaa aatcggtgaa aaaacagcta tcaagctatt gaaacaatat ggctcagtcg
    14401 aaggaattta tgaacatatc gatgaaatga agcaaagcaa aatgaaagaa aatctgataa
    14461 atgacaaaga gcaggctttc ttatcgaaaa aattggcgac catcaatact tcagcgcctg
    14521 tggatgtctc tatcgactca ttgaagtatg aagggaaaaa tctcgataaa ctcgttccgt
    14581 tttataaaga aatggatttc aatcagttcc tttctaaatt gaatatcgta gaagaagaag
    14641 taaaaatgga tgatattttg tttgaagtcg ttcatgaatt caaagaggaa atgtttacta
    14701 cggatatggc gttgtacgtg gaaatgatgg gcgacaatta tcatacagag gaaatcgtcg
    14761 gagtagcgtg ggggacagaa aagaaaattt atgtcacgaa cgatctatct gtattcgaaa
    14821 accgagcgtt ccattcttgg atcactgatc cgactcggct gaaaaaagta tatgatgcga
    14881 aacgaactta tgttgcttta aaccgctata ctggaaaaac gaaaggaatc gattttgatg
    14941 tcttgctagg tgcatacctt ctcgatacga atgaaaaaag cacagatatc gaaggaatag
    15001 ctgctcatta cggatacaac gatatccaat cagatgaagc tgtatacggc aaaggggcga
    15061 aaaaaggttt accagaagaa gaggaactct tttttgctca tttagcgcga aaagtagctg
    15121 caatcaatgc gttgacacca aaattgagcc aagaactggc agaaaaaaac caagaagatc
    15181 ttttccggaa aatggaactg ccattgtcta tcattttggc agagatggaa ataagcggga
    15241 tcaaagtaga tgcgactcgc ttacaagaaa tgaagggcga gttctctgct cgcttaagag
    15301 agatcgaaca aaaaatctac gaagaagccg gcgaagaatt caatttgaat tcaccaaagc
    15361 aattaggagt gatcttattt gaaaaaatgg gattacccgt tatcaaaaaa acaaaaactg
    15421 gatattccac agccgtagat gtactggaac agcttcgtga acaagcacca atcgtagagg
    15481 atatcctcac ctatcgtcaa attgcaaaaa ttcaatctac ctatgtcgag ggactgctga
    15541 aagtgatcca atcagatgga aaagtgcata cccgttatgt ccagacattg acccaaacag
    15601 gacgattaag ctctgttgat cctaatttgc agaacatccc gattcgatta gatgaaggga
    15661 gaaagatacg tcaggcattt gttcctagag aaaaagactg gctgatttat tcttctgact
    15721 attcacaaat cgagttacgc gtacttgcgc atatctcaaa tgatgaacat ttgaaagctg
    15781 ccttcctaga aggtcaagat atccatgcca gcacagcgat gcgtgtattt ggaatcgaaa
    15841 aggcagagga tgtcacacca aatatgcgta ggcaggcaaa agcagtgaat tttggtatcg
    15901 tgtatggcat ctctgattac ggactttcac aaaatctagg aatcagccga aaagcagcac
    15961 agcaatacat cgatacgtac tttgaaaaat acccaggtgt caaagaatat atggaaagaa
    16021 tcgtacgcga agcaaaagac caaggctacg tcgaaacgct gtatcaccgc cgtcgatatc
    16081 tgccagacat caattcaaga aattacaata tccgttcttt tgcggaacga acagctatca
    16141 atacaccaat ccaaggaagt gctgctgata ttttgaaaat cgcgatgatc gaattggata
    16201 aacgattaaa agaaacaggt cttcaagcta cgatgctgtt acaagttcat gatgaacttg
    16261 tcttcgaggt accggaaaaa gaacttgaat cattagataa attggtgaag gaagttatgg
    16321 aacaagctgt ttctcttcat gtaccactga tcactgacag cagttgggga aaaacgtggt
    16381 acgaagcaaa ataaacattg tttaagacag agcaatataa tgaaggtgaa aagaaaaaat
    16441 gccagaatta ccagaagtag aaaccgttcg taaaggattg gaacggttag tagtaggaaa
    16501 aacgattcaa aaagttcagg tcttgtggcc caagatcatc gagcaaccag agacacccat
    16561 ctttgaggct tctcttgttg gcgaaaccat ccaatccatt ggacgtagag ggaagttttt
    16621 aatttttcat ctcgatcatt gtgaactgat ctcacacttg cgaatggaag gaaaatatca
    16681 atttacgaaa gaaaatacac caatcgataa acatacgcat gtacttttct tttttgagga
    16741 tggaagccaa cttcgctaca acgatgtacg aaaatttggt cgtatgacaa tcgtcgaaaa
    16801 aggagcaagt gcaacttacc gaggaatcat gaaattggga cctgagcctt tgccagatag
    16861 cttcttatta gcagatttcg caaatggact gaaaaagtcc cacaaagcaa tcaagccatt
    16921 gctgctagac caacgtctag tgactggtct aggtaatatt tatgtagatg aagcattatg
    16981 ggaagcgaag atccatccag aacaaccggc gaacacactg cgccccaaag aggtagaaca
    17041 attacgtcta tcgatcattg atgttttgga tcgtgcaata gaagccggag gtaccacgat
    17101 tcgcagttat ttgaatgcat taggagaatc aggcggattt caagtagcac tgcatgtcta
    17161 tcagcaaact ggcaaaccat gtatacgttg tggaacgccg atcgtgaaaa caaaagtggc
    17221 acaaaggggg acgcactatt gtccgaattg tcaaaggcaa aaaatcgcaa agtaggattt
    17281 gttttaggtt tgacgggtgg aatagctacc ggtaaaagta cggcagccaa agtctttcag
    17341 tctcatggat ttcctttgat cgatggcgat gtgattgcca gagaaactgt tgagcctgga
    17401 acgcttgctc tgaggaaaat tgtatcagtg tttggccaag aggtattgca gcctgatggg
    17461 caactagatc gagggaaact tgggatgatt gtttttccat caaaggaaaa acggcaaaaa
    17521 cttgatcagc tattagatcc gtttatccga aaagcgataa aagatcaaat cgcctctcta
    17581 agttccaatc atcctttagt aattgtagat atcccattgc tctacgaagg tcattatgac
    17641 cactatatgg atgcagtagc tgtcgtttat actacgccag aaactcagct tcagcgtttg
    17701 atgaaacgta atcaactgac gctagaacaa gcgcagcaga gaattagtag tcaacttcca
    17761 atcgaagaaa aaaagcagcg tgcggatatt ttgttcgaca acaatggaac aaaagaaata
    17821 cttgtgggac aaatcgaaaa ttggcttttt gctaatcgct ttctttgagt atctgtgtca
    17881 attttgctat cctgagtata tagaaagtga gggatagcga tgggatcatt aaaaaatcaa
    17941 aaggataaag tcgtaggtaa cgcaaaagaa aaaatcggcg agatgctcaa taaagaaaat
    18001 ctaatcgatg aaggtcgtac gcaatcagaa acagcagaag aaaaagaaaa gcagtaccaa
    18061 gcaagaatcg atgaaataga agaccagcag gcagaagaag aagcgaagcg gcaagatttg
    18121 actcaagaac gaaaaaatcc gacacctgat cgtagtaaaa caccgctccc agaaagtgac
    18181 cacattcccg atgagcaaaa aatcgaggaa cagcgaatga aacactatac aggaatcgaa
    18241 gagaagttat aaaatagtaa aaaatcgaga tgtgacttga agcagatgag aaggtgttca
    18301 tctgcttcaa gttttttgtg ctagatgact aaatcagctt ttgtgtaagt tatttctcag
    18361 taatcaaggt gatcagtatt ggaaaattga aatttagtaa ggacagggga cacaattacg
    18421 catgaaagta gaaaaaattg ttcttatatc cgtgtttttt gggtaggaca gtcgcgtttg
    18481 tgatttgttt cgtgttataa tacaactagt tgtaaagaaa gacacatcct ttatataaaa
    18541 tgcgatgcaa gaggtgagac aaaatgagat gtcccagatg tcaacataat aattcacgag
    18601 taatcgacag ccgtcaagca gatgatggac gtgcgatccg tcgccgtcgc gaatgtgaga
    18661 attgttcgtt ccgattcact acatttgaac gaattgaagc tgctccgtta ttagtaatca
    18721 agaaaaacgg ggaacgcgaa gagttcaatc gcgataaaat tttaagagga ctcatccgtt
    18781 cagcagaaaa aagacctgta gctatggaac aaatggaaca aatcgtcaac catgtcgaaa
    18841 accgcgtacg cagtctcgga gaaaacgaag tatcgacgaa tctaattggt gaatatgtga
    18901 tggaagattt agttgattta gacgaaatcg cttatattcg ttttgcaagt gtttatcgcc
    18961 agttcaaaga tatgtctgtt ttcttaaaag agttgcaaga tatcgtggat aaagcaaaag
    19021 aaaataataa agagaataat aaagaaaaca attaagaaac aaaaaggagg catttccttg
    19081 gaaaatgctt ggaaagagct tcaaccaaaa aatatttacc aaggaagaaa agcggctccg
    19141 ctgagagaag acagcaagaa cgccttgctg tatctttatc agccactaat cggcggagaa
    19201 gccttgagta tctatttcac cctgttatca gaaatctctc aagaatctgg caaaggtcct
    19261 gaagggatgc atgcagatct gcttagtagt ctaggctgcg gtatcccgca attttatgat
    19321 gcaagaaaaa agctagaagg aatcggctta ctggatgttt attttaaaga agatagtgat
    19381 ctgggacctc gttttattta tgagctgtta gaaccactca ttcctaaagc atttttcaaa
    19441 gactctgttc tggcttttct tcttctggaa aaagttggtg aacgacgatt ccagaagctt
    19501 acccaattct ttgaacctaa aaggcataca gcagagggat atcaaaaaat cacaaaaaaa
    19561 tttttagatg tttatcgctt caatgaagca gtgtacgctg ctaatcaaga aaacatcgaa
    19621 tcgctgaata aatcttttga agaatctgaa tcagtagcat tacttgaagc agattcgaca
    19681 ttcgattggc agtttttgaa tgattggctg gcaaaaaaac atattgcaag tcttgatgat
    19741 ccaatcatca aacaagtcga catgtatcat cagctttacg gtttcgatga attgacactt
    19801 ggcgaactga tcagtcaatc ttatgacttt agtgaacaaa aagtatctga aaaagaactg
    19861 caaaaagcca ttttggcaat gcagagacgt ccggtgaagc aaacaaaaga aacagttgcg
    19921 gtttctgaaa acagtaatga accctcgtca aacgaacatt tgacaaacgc agcgcttcag
    19981 ctgataaaag aagcacagag cattccaccg atgaaatatc tggaagcaat caagtcagaa
    20041 aaaggcggat atgtttctaa acaagaaaac tggttactgc aagatcttgt ttctcaatca
    20101 ggtctttcca gtagcgtgat caatgttttg ctgaattatg tactagtgat caagaataat
    20161 gctagcttaa atgccagtta tgtaaataca gtggcaaatg aatgggcaca aaagaaaatc
    20221 gccacagcag aagaagcgat ccagcacatt cggcaaataa gtaatcaagc aaaacaatca
    20281 aaacaaaaga agcaaacaaa ttatcaaact ggcaagcgaa atgttcgacg ggaaaagctt
    20341 cctgattggg tcaaccagcc aaaagatgaa aaacagatca gcagcgagaa aaaagcagag
    20401 attgaccgcc gttttaaaga atacctggca aaaaaggagg gtgatatcta gtggaagacg
    20461 taggcaaaga attgaagagg atcatcagca aacgcaatta tcaagaaaag tatgctgaaa
    20521 tgatcgctga agtcttaaat gatgcggatg ttcaagcttt tctaagcgca caccaagaag
    20581 aattgacaca agcggatatt gaaaaaagtt acgctaaatt gtatgaattc gtgcaagaaa
    20641 agcgtaaata tcaaaaaaac gatccaacga tgatcgctcc cggctatgag ccgaagctga
    20701 cgttgaattt tcattatgtg gatgtcactt atgtaccaac ggaagcattg ttagcacggc
    20761 aaagacaaga agaaatcaga agccgagtaa aagcaatgga tatgccaaaa gatattcaat
    20821 cggcttcttt tgaaaacttt gaacgtacag atggacgagg atatgcgagc ttagaagccc
    20881 tagattttgt agagaaatat actgctgatc caaaagggta tcataaagga ctgtatctag
    20941 taggaagttt tggtattgga aaaacatatt tattaggagc aacagcgaga gaattagcga
    21001 ttgctggatt caccacgacg ctagtccatt ttccgacatt tgccgttgag atgaaacagg
    21061 caatcggaaa agatcaagtg gcagaaaagt tagatgcagt gaaacgttca cctattttga
    21121 tgatcgatga cattggtgca gatgcgatga gtagttggat acgagacgaa gtattcggtg
    21181 ttatccttca atatcggatg caggaacagt taccaacctt cttctcatcg aattttacaa
    21241 tggatgaatt ggaacagcat ttgtccgtca ctcaacgtgg agatgaagag ccgctaaaag
    21301 cgaaacggat aatggaacgc atacgttatt tgacaaaaga aatcgaaatg actggtcgca
    21361 accgacgcaa tggttgaaca gttgacaagt ccaactgaca tttgacctag tagtaaaagc
    21421 aggtcagcga atcattatat ggacagaaat gaccggagac aatcaccgca acaagtaatg
    21481 cagaaactta aacggaggat tttttatgag ggctattttt tctagtatat ctgtagttgg
    21541 tctatttttt cttttagcag gctgcggtag cgcagatagc aacacaggtt ctgttgattc
    21601 gattgatgcg ggtcaaacag taaaaacaag cagtacgaaa acggagagac aaagcatatt
    21661 tgtcggaacg attgaaagta tcactcaagt agaagcagaa gaagaaacgc ttcagttgaa
    21721 actcgtcgat gtcatggcca aagaagatcc agcgaatatg ggaacgtcat tcagcaatga
    21781 cggagtgatc ttaaatgcta ctgctgaaaa aataagcgtg gacataaatg aattgaaaat
    21841 cggtgacaaa gtccaattta cattagttga gatgccgatc atgacgatgt ctatcccacc
    21901 gcaagttcct ggagattcga ttattgatgt ttcattgaac aaataaccaa taacattttt
    21961 tcaaaaataa gaaaatgttg ttttatagaa cgaaaaggta tattttttaa ggagatatgg
    22021 gaatttttt
//
LOCUS       DACDIH010000040        21784 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDIH010000040 DACDIH010000000
VERSION     DACDIH010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.040"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            132..3659
                     /locus_tag="IV281_001660"
     CDS             132..3659
                     /locus_tag="IV281_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8780651.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3656..7378
                     /gene="addA"
                     /locus_tag="IV281_001661"
     CDS             3656..7378
                     /gene="addA"
                     /locus_tag="IV281_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP8780652.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7447..7788
                     /locus_tag="IV281_001662"
     CDS             7447..7788
                     /locus_tag="IV281_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8780653.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8097..9140
                     /gene="pheS"
                     /locus_tag="IV281_001663"
     CDS             8097..9140
                     /gene="pheS"
                     /locus_tag="IV281_001663"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP8780654.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9145..11565
                     /locus_tag="IV281_001664"
     CDS             9145..11565
                     /locus_tag="IV281_001664"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP8780655.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11735..12745)
                     /gene="trpS"
                     /locus_tag="IV281_001665"
     CDS             complement(11735..12745)
                     /gene="trpS"
                     /locus_tag="IV281_001665"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP8780656.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12999..13736
                     /locus_tag="IV281_001666"
     CDS             12999..13736
                     /locus_tag="IV281_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8780657.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13750..14565
                     /locus_tag="IV281_001667"
     CDS             13750..14565
                     /locus_tag="IV281_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8780658.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14578..15225
                     /locus_tag="IV281_001668"
     CDS             14578..15225
                     /locus_tag="IV281_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8780659.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15241..15897
                     /locus_tag="IV281_001669"
     CDS             15241..15897
                     /locus_tag="IV281_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8780660.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16059..16880
                     /gene="racE"
                     /locus_tag="IV281_001670"
     CDS             16059..16880
                     /gene="racE"
                     /locus_tag="IV281_001670"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP8780661.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16883..18238
                     /gene="rph"
                     /locus_tag="IV281_001671"
     CDS             16883..18238
                     /gene="rph"
                     /locus_tag="IV281_001671"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP8780662.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18239..18757
                     /locus_tag="IV281_001672"
     CDS             18239..18757
                     /locus_tag="IV281_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP8780663.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18864..19364
                     /locus_tag="IV281_001673"
     CDS             18864..19364
                     /locus_tag="IV281_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8780664.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19644..20564)
                     /locus_tag="IV281_001674"
     CDS             complement(19644..20564)
                     /locus_tag="IV281_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP8780665.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20729..21742
                     /locus_tag="IV281_001675"
     CDS             20729..21742
                     /locus_tag="IV281_001675"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP8780666.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
BASE COUNT         7300 a         3770 c         4658 g         6056 t
ORIGIN      
        1 ttttgcgtat acttaaaaaa gtagaaatat cagaacctta aaaggatata atttgaagaa
       61 tatagaacaa atgttcagta ttttttgata taataaagga gcaccgtatc atgtgttgaa
      121 ggaggaaaag catgtcttta caatttattc ttggaaacgg gaaaaaagat caccgtcaag
      181 tcttgatcga cgaagcatcc gcttggctcg accaggatgc aagtaaccaa gtatttttcc
      241 ttgttcccaa ctacagcaaa ttcgaacagg aacaagaaat tttagcggag atgcgaagaa
      301 gaagcggaaa aaaaactttc agcacaacaa acgttcaagt atttagtttt tatcgtctgg
      361 cttggtattt gctgcaacag acaactcttc ttacaggaaa tgaattatca gaatcaggat
      421 ctgcaatgat cttgcgacag attttagaaa aaaatgagga agaattaaca atctttcgcg
      481 gagaaatcaa taaaagaggg tttattcgtc aattacaaga actctacagt gaattgcgta
      541 caggaaatat tcaaccagaa gacctgatgc ttttatttgg tgattcacct aatgcagaag
      601 accagcaact taaaatgaaa gacttgaagc tgattttttc agcttatgac agcgaattga
      661 ctcaacgtgc actaacaaat gaagatgcac tatctatact agcaaattat atgcagacac
      721 aagatttcaa caacgtcaaa tttattgtca gtggtttttc tcgaatcaat ggtcaagagt
      781 atcaattact gcaggcaatg atggaaaagg ggcaattggt tgtcgattta ttgctggatc
      841 gtccttatac agcggatctt ccagaagtac ttacgctctt tcatgagact ggaaagcttt
      901 atttccgtct ttttcaaagt gcaagagaac ggaaagtccc tatattagtg gaccgatttg
      961 caccagagca gactcgtccg acagagcttc aactgctgca aacaatgtgg gaagagacat
     1021 ctagccaaaa gaaagcggca gtacacaaaa tagggggaca agagcatctc catttgtggg
     1081 aagctgaaaa tccaactgaa gaagtccgac aattagctgt tgaaatcaga cgactagtca
     1141 gtgaagaaaa ctaccgttat cgtgatatcc aagtgctgac aaaaaatatg tctttatatg
     1201 gaaatatcct tcggccgatc tttcaagaaa tggcaattcc tttttatatc gatgaagaac
     1261 aaatgatgga aaatcatccg ctgatcgaat ggatcaacag tctgttcgct cttgaccgct
     1321 attcttatcg gctaagtgat attatgcgtt ttttcaaaac tgaattattt ctgtcgacgg
     1381 aagcagaaac cgaacttgat gaatggaaac aagagcgaga tgaattcagg aaaaaactgg
     1441 acatcactga aaatgtagca cttgcttaca attatcaagg aacttattgg actcgtgaaa
     1501 aagactggca gtttgtggcg tatgattttg aagcagaaca gattgaagac gtacagtatt
     1561 tggaagaaca gtcaaatacg attagacgat tatttcagaa agctgtacca gtatttttga
     1621 aaaaaataaa gaaaagcaaa acaggacaag aggccgcaaa tgttttttat caatttttga
     1681 tccaaagtgg cgtacagcaa caattattgt tttggcgtaa tcaggaagta gacagaggaa
     1741 acttagaagc ggcaagaaac catgagcaga catggggagc gctgattgat ctgttggatg
     1801 aatatgtagt aatttatggt gaatcggctt ttgactggtc actgttccaa gaaatcatta
     1861 caagtggtct agaaaatcta agttatggta aaatccctac cgcaatcgac caagtgcgaa
     1921 tcaatcgttt ggaattagtt cgaccaaatc aagctgccgt cacttttgca ttagggttga
     1981 atgatcaagt gtttccagac cgaaaagaga caaaaggatt gttgtccagt gaagaacgag
     2041 aagtattaaa tcaggccttg ccagatggac agtttttatt cgacccatca aaggaaagta
     2101 tctcttttga gcctttccaa gcctatcttg tttttctttc cggaacagat cgattatatc
     2161 ttagttatgc acaaagctat gacacagatg gttcgctgaa gatgtctcct tatttgcgta
     2221 gaatcacaga atatttatct gttcccgtcg agagaaaaga gcatttgacg atcgaaagcg
     2281 accctaattg ttatgtaggg acttacagaa gcggaatcaa tacagtcaat aggatttatc
     2341 gattagcttt agatgaaaaa aaacctgtac caaagcactg gcaaaagctc aaagaattag
     2401 tactgaattc gtcatggcga aattttgctt tacagatttt tgaaagtcag gaacatttaa
     2461 atgttcctgt ttcgatgcca gaaaaaatgg cggaagagct ttatggaaaa gagatttata
     2521 cttcaatttc acgtatggag aatttttata attgtgaata caaatacttc atgcaatttg
     2581 gtttgaaatt gaaagaacga actgtttatg gtctaactcc agcagcaact ggtgattttt
     2641 atcatgaatc attggatcgt ttcttcaagc tactgttttc aaaccaatta tccttagtga
     2701 atatgtccga tgcgcagcgt agagacttta ctgaaaaagt gctgcaggaa gtattcggag
     2761 aacttcggtt tgagatattg gatagttctg caagaatgaa ctatatccgc taccagttag
     2821 gacagacgat tcaaaaagtg acatgggctt tgcaaaaaca gagcaaaaat acaggcatgc
     2881 atcctcttca gacagagatt ctatttggtc agattgctgg agcaaaaggt atcacaggtc
     2941 tggaattacc gttgagtaat ggtggaaaac tccatgttcg agggaaaatc gatcgaatcg
     3001 atgtagcttc tgaacaagaa gatacttggc taagcgtcgt tgattacaaa tctagcggac
     3061 gttcattcga tgtcacagaa gcttattatg gcatggctat gcaattattg acgtatctgg
     3121 acgtagcatt gatggatgcg gtacgtttga ctggaaaggc ggctgttaaa ccagcaggat
     3181 catattattt acatgttcat aatcctgtat tgacggagac agatgatgta gagaagaata
     3241 cattgaaaaa atttagttat gatggcgttt ttgtcgatga tcccgctctt ttagaagtac
     3301 tagatcattc actggaagca aaagaacaat cactgatcta tccagtgaaa aaaaatgcga
     3361 aagatgaata ccaaaaaggt agtttcagta aagacaaatt tttcacggaa gaagaactgc
     3421 aactattcat ggcgcataat cgggaaaata tgcggttagc tggagatgcg attacttccg
     3481 gagaaatcaa actaaatcca gcttataaag ataaacagcg gattgcctgc gaattttgtc
     3541 cattcagaag catctgtacg tttgatgtga tgctgaaaga aaataattac caccgtctgg
     3601 agaaaataga taaggaagaa gcactgcgta gaatattgaa aaggagtgaa gaagagtgaa
     3661 tcaaattcct ttaaaaccgc aaaatgaacg attcacagat gatcagtggc aagcgatttt
     3721 tgatcaagga gataatctat tagtctctgc gtctgccgga tcaggtaaaa ctacagtgct
     3781 tgttcgaaga gtgattgaaa aactaaaaat gggttttgat atcgatgaat tattgattgt
     3841 gacatttact gaagctgccg caagagaaat gaaagaaaga attcaagagg cattacaaga
     3901 atccgtcaat agcgaaagtg accctgtacg tagacagcat tttactaagc agttagtttt
     3961 attgcccaca gcgaatatct caaccttaca tgctttttgc ctgacagtta ttcgacgtta
     4021 ttattactta attgatattg atccagtctt tcgcatgtta acagatgaaa cagaaacgat
     4081 cttgatgaag gaggatgtgt gggacgagct gagagaagca ctttatgcag aaaatgatga
     4141 acgatttttc caattaacaa tgaatttttc taatgatcgt tcagatgacg gcttgacgaa
     4201 tctggtgttt tctctttatg agtttgcccg tgccaaccct gacccccaaa aatggctaga
     4261 acagttaagc gataattatc gtttgcctga ggggctagcg aaatctcgat tatatcaaaa
     4321 acaaatccgt cctttagttt tggcagatat ataccaatgt gtacagttat atgaacagat
     4381 gactcagtta gcacaaggtg aaggattaga aaaaatgcat gagcaagtag ctggagaaca
     4441 acagcagatc aagaatatct atgaagcgtt ctcgcaagat cggttagaag aagcttatgc
     4501 tggtttggaa caactaactt tttcaacttt taagagcagt cggaaagcag aattgaaaga
     4561 aatttccaat gaagtcaagg gcatgaggga taaagcaaaa aaactgattc agcagataag
     4621 taaaagttat tttcctgttt ccccaagcca aatggaagaa ttaacggaca aagcactacc
     4681 gttagtcgaa gaaatgacaa aagtcacgca aagcttcatg gacggtttca gtatgcgtaa
     4741 acgggaaaaa ggtgtgctgg atttcaatga tctggaacat ttagcgttgc agattctcac
     4801 agaaaaaacg aaagatgtat ggctgccaag tgaagcttca aaacattatc gaaaaaaatt
     4861 caaagaagta atggtagatg aatatcaaga cgtgaaccaa ttacaagaag ccattttata
     4921 ctggctaaga gaacccgatg atacaaaagg aaatatgttc atggttgggg atgtcaaaca
     4981 atccatctat tctttccgtt tagcagatcc tagtttattt atcgggaaat acgagaattt
     5041 ctcaaagaaa gaaggcggac gtcgaatcgt tttggctgag aacttccgct ctagaaaaga
     5101 agtcctttct tttacgaatc ttatttttga acaactaatg gatccagcag tgggtcaaat
     5161 caattatgac gaagcagcca aactgatcca aggattttca gactttccag agaatgaaca
     5221 gtttgagccg gaaatcatga tctatgaaaa agaacaggaa gaatcagaga ttgagattcc
     5281 gacggatgat atactggaag acaaaacaga aggcgaactt tttatgacag gactaaaaat
     5341 tcgtcaatta attgattcct cctttatgat ttatgataaa aaatcaaaaa aaagtcgtcc
     5401 gatagagtac aaagacattg ttctcttgac acctacaaag aagaacaact taacgatttt
     5461 agagattttc aaaacattgg atattccatt agagatgaat gatgctcaaa actattttca
     5521 agcgacagaa attcgcacga tgatctcgct gctacaatta atcgataatc cctatcaaga
     5581 tattccgctt gcggctgtat tgcgctcacc gatcgtgggt ctgattgaac cagaattagc
     5641 cagcattcga ttagctaacc gtgcacatac ttactatgat gctgtgcttg cttatcaagc
     5701 atcaaacgaa gatgaattag cagctaagct ggaacatttt ggcaagcaac tagagcattg
     5761 gcgagaactt gctcgccgtt cttcgatcac tgacttgtta tgggatatct attatgagac
     5821 gggatatcta gagtatgtgg tcggtctgcc agcaggggca cagagacaag ccaatcttta
     5881 tgcattggtt gatcgtgcca aagcttacga gcaaagttct ttcagaggat tataccagtt
     5941 cgtgcgtttc attgaaaaaa tgcaagaaaa agataaagac ttagcagaac cagtcatttc
     6001 aatagaagat aatgctgttc gcgttatgac gatccatgca agtaaaggct tggaattccc
     6061 agttgtattt ttgttagata tgacaaagga gttcaactta caagacttgc gcaatcggta
     6121 tgcttttgaa gaaaaacttg gagcaggaat tcgctatatg gatcctgaaa cacgagtgtt
     6181 atatgatacg ttaccgtttc aagccatcaa attggcaaag caaaacaaat tgttgtcgga
     6241 agaaatgcgt aaattgtatg taggattgac tcgggcagaa caaaagcttt tcattgtcgg
     6301 ttcctataaa aataaagagc aaatgatcca aacgtggtct gaagcagctg atcatgagga
     6361 gttagttttt gatcctgcgc tacgtttgaa gggcagaagc agcttgatga attggattgg
     6421 ttacggattg atccgtcatc ctgaaatgca gaaatactta gaggaagaaa tttcgacttc
     6481 tcttcttcaa cattcaaatg cacagttttc tatttcatgg atgaatcagc aatcgatcat
     6541 tgaacaaaga cagttgcttg ctgaaaaaga actggttaac ttagatcagc agatgaaaga
     6601 agatgaaact ttattagcag attcgttaca aaaacgtctg gcatatgaat atccttatca
     6661 agcttcttca caaaccacaa gttatcaatc tgtatctgaa atcaaacgat tattcgaaga
     6721 tccagatgat acacaagaat cacgtttgac tctggaaagc agtcagaaca aagcagcatc
     6781 ccgtcaattt cgttatacgc aagaacaact agcagaacca aaatttttgc aaaaagatcg
     6841 gcaagtgtca gcagctgctg ttggaactgc tacgcatgct ttgttacaat tactgccatt
     6901 agagatgcca actacagaga gtatccatca aaaattacag gaattggtaa ggaaacggtt
     6961 agtagacgaa aaagtcgcga aaaaagtaga tgtttcttct attatatggt tctttcaaac
     7021 agaacttggt caacagctga ttgccaacaa agaaaacgta aaacgagaac aacctttttc
     7081 catgctgctt cctgctgacg aagtatttca agactatcca aatcaagaag atgaactatt
     7141 aatccacgga atcgtcgatg gatacctaga agaaaaagat catctcaata tctatgattt
     7201 taaaacagat tttattttac atccggatga tccagcagaa atagatgcaa tcgtccaaaa
     7261 atatcaagga cagctaagac tttaccaaca agcaatgtca gaagctttga ataagccagt
     7321 agaaaatgtt ttcctaattt tgcttcgagt gaaacaaatt ataaacatta acaaataatt
     7381 ataattgagc aaacttgact tatgttttgt aaggattatg ctatactctg ggaagaggtg
     7441 agtatgatgg agctaacaca gacagaattc gttttatgcc ctaaatttga aaaaacattc
     7501 tctattttag gaaaaaaatg gaacggattg attatcgatg tactactcga aaacggtccg
     7561 caacgctttg ttgagcttgc ggcaaaaatc ccgatggtca gtgatcgagt attagtcgaa
     7621 cgcttgaagg aattagaaaa agaaggaata gtcactcgaa actttgcctg tagcgaaaag
     7681 agccgttcag aatatctctt gactgaaaaa ggagcagact tgcaaaaagc gatgcgtcaa
     7741 atacaagttt ggagcgaaaa atggatcaca gaggaagaat gcggttgatt ttgtaaaaaa
     7801 aatcttgtaa actatagtcc atacgaataa gtaaagaacg atgacgaaaa agagtagttt
     7861 ggctaaacca gttcagggag atctgtcgca gactgaaagc agatcctgtg tttgaaaaat
     7921 gaagttcact ttccgagttc gtctgataaa atcagaccgg caattgtcgt taacgaagta
     7981 agagtgcggt gtaagtcatt tatccgcaaa ttaggatggt aacgcgaaga cctcgttcct
     8041 taacaggaat gaggtctttt tgttttgtat ttggaattta aaggaggaac aagcacatgt
     8101 ctctacaagc acaattagag agcttgaaag aagcaacgct cgtcaaaatc acagcggcac
     8161 aagatttaaa tgaattaaat caaatacgtg ttgaaacgtt aggaaaaaaa ggaccgatca
     8221 cagaagtatt acgtgggatg aaagatctaa gtccagaaga gcgaccaaaa gtcggaagtt
     8281 tcgcaaatga gatccgagat cttttgacag aagagatcgt gcgaaaaaaa caagtattag
     8341 aagaagaagc gttaaatgca gctttggcaa aagaaacaat agacgtgaca ctacctggta
     8401 gaaaagtaaa acatggtaca cgtcatatcg tgacacaaat catggaagaa attgaagata
     8461 tttttgtggg tatgggctat cagatcgtag aaggatatga agtagaatct gatcattaca
     8521 atttcgaacg gatgaatctg ccaaaagatc atccagcaag ggacatgcaa gatactttct
     8581 atatttcaga cgagatattg attcggacac atacttcacc agtccaagca cggacaatgg
     8641 aaaaacatga tttctctaag ggtgctctac gaatgatctc gccagggaaa gtcttccgca
     8701 gagatacaga tgatgcgacc cacagtcatc agttccatca aattgaaggg ctagttgttg
     8761 ataaaaacat cacaatgggc gaccttaaag gaacattaga agtcgtaatg aaaaaaatgt
     8821 ttggggaaga tcgtgaaatt cgtttgcgtc caagttattt cccatttacg gaaccatcag
     8881 tagaagtaga tgtcagctgt ttcaaatgtg gtggtgccgg ttgtaacgta tgtaaataca
     8941 ctggatggat cgaaatttta ggagctggca tggtacaccc gaatgtgttg aagatgtcag
     9001 gaatcgatcc agaagaatat tcaggctttg ctttcggcct tggaccagac cgcgtagcta
     9061 tgttgcggta tggagtgaat gatatccgta atttctatca aaatgactta cgtttcttaa
     9121 gtcagttcaa gggggagtaa gaaaatgtta gtttcatata aatggctcaa tcgttatgta
     9181 gatttatcga atgtaacacc aaaagaatta gcggataaga tgtctgtgac aggaatcgaa
     9241 gtagagggag taacggttcc agaagaaggc ttgaaaaaaa tcgttgtcgg tgaagtcaag
     9301 gaatgcgtac cacatccaga ttctgatcac ctttctattt gtcaagtcga tattggcgaa
     9361 gacgaactgt ctcaaatcgt ttgtggagcg cctaatgtga aggttggaat caaagtgatt
     9421 gttgcacttc ctaactcacg tatcgctggt aatgtaaaaa tcaaaaaagg aaaaatgcgt
     9481 ggccaagtat caaatggcat gatttgctca ttacaagaaa ttggctattc agatagtgtt
     9541 ataccgaaag aatacgctga aggtatctat tatatgccac aagaagctgt aaatggcgat
     9601 ccagtttttc cttatttgga tatggatgat tcgattattg aattatctat cacaccgaat
     9661 cgtgcggatg cattgtcaat gcgtggcgta gcttatgaag ttggggcaat ttatcgtcag
     9721 actcctacat tcgatgacga aaagctggta gaatcctcac gtcctgctag cgataaaatt
     9781 caagtggaag ttgaagataa agaagcagtt cctgcttatc aaatcagaat cattgaagga
     9841 gtaagaatcc agccaagtcc tcaatggctg caaaatcttt tgatgaacga aggcattcgt
     9901 ccaatcaaca atgtagtaga tgtgacgaac tatattttgc ttttattcgg acaaccacta
     9961 catgcatttg actacgacaa attgaaatca aaagagatct ttgtccgccg tgcaaaagaa
    10021 aatgaaacat tagtaacact agatggagaa tctcgggaat tgacaactga caatctcgtc
    10081 attactaacg gcaaagaacc agttgcttta gctggtgtca tgggagggtt agactcagag
    10141 atcacgaatg aaacaacaac agttgcacta gaatctgctt tatttgatcc aatttcgatt
    10201 cgtcgtacag caaaacaatt taatttgcgt agtgaatcct ctgcacgttt tgaaaaagga
    10261 atcaaccatg cgactgttgg agaagccagt gaggttgctg cagcaatgat cgtttctctt
    10321 gcgggtgggg aagttctaca aggcgcggtc aaaggctcag agatcaaagc agaagacgta
    10381 tcagtgaaga tttcacttga acgcatcaat caatatttag gaacatctct tgtcgttaaa
    10441 gaagtaaatg agatttttga agcacttgga tttagctatg aagaaaagga gtatgtttat
    10501 tccgtagtag taccaccaag acgctgggat atccaaatcg aagcagatat tatcgaagaa
    10561 gttgctcgta tttatggata cgaccagttg ccatctacac ttccaagtgg agaaacggtt
    10621 gctggaagct tgtcaaaaga acaagaaact actcgtaaaa ttcgtacaat ctttgaagga
    10681 agcggattga gcgaagcaat cagttatgca ttgacaacag aagaaaaatc aagacagttt
    10741 gttataaatg aaagtaatat tacacgtttg gactggccaa tgagcgaaga acgttctgta
    10801 ttacgaatga atttgatcag cggactatta gatgatatca gttataacac tgcccgaaaa
    10861 aatacagaga ttgctttata tgaaattggt cgggtgttct tccaagaaaa tgatccacgt
    10921 acacatctgc cgcaagaagt aaaacatgca gcaatcgcta tttctggtat ttgggaagaa
    10981 aaagattggc agacgaaagc tcaaaatgta gactttttca ccatcaaggg tcttttagaa
    11041 aacttatttg aacaactagg catcactgaa gatgtccatt atcaaacagt gacagaaatg
    11101 aaggaaatgc atcctggaag aactgctgcg atttaccttg gcgaaaaatt tattggtttt
    11161 gtcggtcaag tgcatccaac gattacaaaa agttatgata ttccagaaac atatgttgct
    11221 gagtttgatt tatctgctat agtggaagct gctcaaaaag ggatcgtttt cgaagcagtg
    11281 acaaaattcc cagcagtcag ccgggacatc gctttgcttg taaaagaaac tgtgacaaat
    11341 caggaattaa cagaagttat ccgttctgct gccggaagat ttttaacaga tatccaatta
    11401 tttgatgtct atcaaggaga aaacattgaa aaaggacata aatccatggc gtatagcctg
    11461 acattcgtca acccagaagc aacattgaca gatgaagaaa tcaataaagc aatggaaaaa
    11521 gtcgaaaaag cgttggtaga aaacctagag gcaagtattc gctaagaagg aaaatatagt
    11581 aagtaacgat caagtagctt ttgaagacca tacatttgaa aaaaagatgt gacacaactt
    11641 cgtcacatct ctttttttga ttttaaaaaa ggaatagcac agagacgaga taaaaaaaca
    11701 ggcctttaca gcctgctttt ttagagtaaa tgaattaatg gaagtaattg attcccattg
    11761 ctgattttac ttcagaaagt gtttgtgcag ctactttagc agcttcttca ctacctttac
    11821 gtaagatttc cataacagct gaaggatctt tttccaattc ttctcggcgc gcacggatcg
    11881 gtgcaaattc agcttccaat acatcaatga ggtatcgttt gatctttaca tcaccaagtc
    11941 cgccgcggcg gtaatgagct ttcatttctt caattgcttc tttatctgtt ccgaatacat
    12001 ccaagtaggt gaagaccata ttcccttcta cttgtccagg atcttgtaca tggatgtggt
    12061 ttggatcagt atacatactc atgacttttt tctgtaagac atcggcagga tcggagatat
    12121 aaataccgtt tcctaacgat ttactcattt tgccatttcc atcgattcca ggtaatctac
    12181 ccattccttt tggtgggaat acgccttttg gttccaccaa gacttcacca tatgtatgat
    12241 tgaagctttg tacgatttcc tgtgtctgct ctagcattgg tttttgatcc tcaccaacag
    12301 gtacaagatt cgctttgaat gcagtgatat ctgctgcttg agaaacagga tagataaaga
    12361 atcctgtagg cacgctttca ccgaattttt tttgttcgat ttctgttttg acagtcggat
    12421 tgcgccgaac gcgtcctacg ctgactaagt tcaaataata catggttaat tcagctaatt
    12481 ccggaatttg tgattggata aataaagtag attttgcagg atctaatccc acggctaaat
    12541 agtctaaagc aacttgcaag acatttgatg aaaccttctc aggattttta gcattatctg
    12601 tcagtgcttg catgtcagca atcatgacaa acagttggtt gtcttcatct gcctgcatag
    12661 ccactcgggt ttttagagaa ccgacgtaat gccctaaatg cagcttccca gttggtcgat
    12721 ctcccgttaa aataatattt ttcaaaaaag acacctcttt acgatttgta agtctgagca
    12781 cttaatatct taccgttttt cctgaaaaga aacaacagtt cctaaggaaa aaacaaggaa
    12841 agaggataaa ataactaata gattagaaat ggaagaaata tttatgggag aataaacgtt
    12901 ctaagaaact ttacaaatta ctaaactatc agatataatg tattttatta tacatcttta
    12961 aaagaaatct tactaaataa taatgggagt gtaattctat ggcaatgatt aaatttgatc
    13021 acgtggataa atattatggc aagttccacg cattaaaaaa tatcaatctc gaattcgaga
    13081 aaggagaagt tgtcgttgtc attggtcctt ctggttcagg aaagagcacg atgcttcgct
    13141 gtatcaatgg attagagacg atctcttcag gtaagctgct gattaatgat actgacttgc
    13201 atgataaaaa gacaaaactg acagaagtcc gaaaaaacat cggcatggtt ttccaacatt
    13261 tcaatttgta cccaaataaa actgttctgg aaaacatcaa attagcacca atcaaagtgt
    13321 tgaagcaaga ccaggcaact gcagtcaaaa acgcggagaa atttttgaaa actgtcaata
    13381 tgttagataa gaaagattct tatccttcca tgttatccgg gggacaacag caacgggtgg
    13441 caatcgccag aggtttagcg atgaatcctg agatgctgct gtttgatgaa cctacttctg
    13501 ctttggatcc ggaaatgatt ggtgatgtat tagacgttat gaaaaaattg gctcatgacg
    13561 gtatgtcaat gattgtcgtg acacatgaaa tgggctttgc caaagaagtg gcggatcgtg
    13621 ttatcttcat ggctgatgga gaagtattgg aagacagcag aaatgtgaga gactttttcg
    13681 aaaatccaaa tgaagtccga gcacagcaat ttatcagtaa agttatcaat cattgatagg
    13741 aggaaaaaga tgcgcaggtt aaaaaaaata cgcctgattt tccttttagg cattgctttt
    13801 cttgcactag ccggatgtaa aggaaatggg ttggcaaatg aagatatttt agagcgaagc
    13861 aaatctacca atgaaatcat ctggggtgtc aagtatgata ctcgattatt tggaatgatg
    13921 gatattgaga gcagaactgt acaaggattt gatgtagata ttgcaaaagc aatcactaaa
    13981 aagatccttg gtgacaatgg aaaaacagaa tttgttgaag tcacttctaa aactcggatt
    14041 ccattattga aaaacggaaa tatcgatgca attattgcaa cgatgacgat cacagatgaa
    14101 cgaaaaaaac aggttgactt ttcagatgtc tatttcgatg ctggccaagc tttgttggta
    14161 aagaaaggaa gccaaatcaa aagtgtagat gatttgaatg cttctacaac ggttctagca
    14221 gtcaaaggtt ctacatcggc tgcgaatatc cgacagcatg caccagatgc aaaaatttta
    14281 gaattagaaa actatgcaga agcatttact gctttacaat ctggtcaggg agacgcgatg
    14341 actacggata atgcgatttt gttagggatt gcagatgaaa atccagagta tgagttagtt
    14401 ggtggaacgt ttactaatga gccttacggg attgcgatca acaaagggca agaaaacttc
    14461 ttgaaagctg ttaaccaagc gctagaggaa atgcatgcag atggcacata cgataaaatc
    14521 tatcaaaaat ggtttccaaa tgaaactgaa ggaaaagtag agtaaggggg agaggcaatg
    14581 agcgaattga tacaaactta tggaccaatc tttgtcgacg ggttcaagat cacattgttt
    14641 tcgagtgtct tagcacttgt gtttagtttg attatcggaa cgttaatggc aatcttccaa
    14701 ttatcaaaga ataaagtagt gagcgcctta gcaaaggcat atgtagaatt ttttagaaac
    14761 attccattat taatcatcgt gatgtttttt tatgttgtcg taccgattta ttggtttaat
    14821 ctagatggat tccaagcagg tacgattgga ttaacgatct atacatctgc ttttattgct
    14881 gaaacagtac gttcagggat tcaaaccgta ccaaaaggtc aaacagaagc aggactatct
    14941 tctggtttta cctattcaga aatcatgcgt tatattgttt taccgcaagc attcaaaatc
    15001 gttatcccgc cattaggcaa ccaatttatc aacctcgtaa aaaactcatc tattttagca
    15061 atcgtagctg gtttagattt gatgtatcaa ggagacttga tcgctagtga aacattcaat
    15121 accttcgata cgtatatcat cgttgggtta ttttacttag ttatcacgct accgttatct
    15181 tacttaatgg tttatcttga gaaaaaatgg gcggttcgtt catagaagga ggggaaatac
    15241 atggatttta gcggcgcttt ttcatggatg aatatccgtt ttttattgga aggattaaag
    15301 gttactgttg aagtttcatt gttttcgatc atttttagtt tcttgatcgg tggtctgaca
    15361 ggtgtgattc gttttgcgaa tatccctttc ttttccaaaa tccttggtgt agtgattgat
    15421 attatacgga atcttccatt attattgatc attttcttca cttactttgc cttacctcaa
    15481 atcggcattc agatgaacat cttctggtcc gctgtcgcag cattaacgat ttttgagtca
    15541 gctatgttgt cggaaatttt ccgagctgga ctcaatgctg ttccaaaagg acagatggaa
    15601 gcaggattat caacgggact gacttatgta gaatcgatgc gaacgatcgt gttaccgcaa
    15661 gcatttaaat caatgatccc ggcaattgtg agccaattga tttcactgat caaagatact
    15721 tcattagcag taatcatttc tttacctgaa ctttctcatc aggcacgtat tatttatgga
    15781 cagaacacca attatgtgct tccgatgttc atcatcatga cacttatgta cttcgtggta
    15841 tgttacgcgc tttctctgct ttccggttac ttggaaaaga gacggtacag ttattaatag
    15901 atgattcttt tagcggttgt ggcaaactaa agtcacaacc gcttttcctc tttccattcg
    15961 aaaaaaagag atttcgattg ttttaagcaa gtaagagcac ttgaaaattc acgctaaaaa
    16021 gtacacaata gggaggatag aatttattga aaaaaaggat gatacgattg acagataatc
    16081 gccctatcgg atttattgat tcaggtgtcg gcggcttgac tgtagtaaaa gaagccctga
    16141 aacaattacc gaatgaaaat attttatttg taggagacac agcacgctgc ccatatggcc
    16201 ctagacccgc ggaacaggta atccagtata cttgggaaat gacggattat ctggtggagc
    16261 aaggaatcaa gatgctggtg atcgcctgca ataccgcaac tgcggtggct ttagaagaaa
    16321 tcaaagctgc tctttctatt ccagtcatcg gtgtgatcct tcccggtact agagcggcag
    16381 taaaaaaaac acaaaataaa caagttggca ttatcggtac gattggtacg gtaaaaagtc
    16441 aagcttatga aaaagcactg aaagagaaag taccagaatt gactgtgaca agtcttgctt
    16501 gtcccaaatt tgtttcagtt gtcgaaagta atgaatacca ttcatcggtg gcgaaaaaaa
    16561 ttgtggcaga aacattagct cctttaacca ctaaaaaaat cgatacattg attttgggat
    16621 gcacccatta tccattatta cgccccatca ttcaaaatgt aatgggagaa aatgttcaac
    16681 tgatcgattc tggagcagaa acagtaggtg aagtatctat gctgttagat tatttcaatt
    16741 tgagcaattc accgcaaaat ggtcggacat tatgccagtt ttatacaact ggctctgcca
    16801 aacttttcga ggaaatagct gaagactggc ttggaatcgg acacttaaat gtagaacata
    16861 tcgaattggg aggaaaataa ggatgcgtca tgacgggaga aaagcccaag agcttcgcaa
    16921 actggagctt caaacaaatg ttttcaaata tccagaagga tcggtagtga tccgttttgg
    16981 tgatacaaca gttatttgtt ctgcaacaat agaaaattct gttccccctt ttttgagaga
    17041 aacaggaaca ggatgggtga cagcagaata tagtatgctg cctcgagcaa cgagtacgag
    17101 aaatcgtaga gaaagcagca aagggaaact ttctggtaga acaatggaaa tccagcgtct
    17161 tattggtcgt tccttgcgtg cagtagtaga cttggaaaaa ttaggagaac gtagtatcat
    17221 cgtagactgc gatgtgatcc aagcggatgg cggtactaga acagcaagta tcactggggc
    17281 atttgtggca ttgaaattag ccattgaaaa acttttaaga gaaaaagaat tgtcagaaga
    17341 tccaataaaa gaacatcttg ctgcagttag cgtaggaatc ttaccagatg ggacttgcgt
    17401 cacagatttg gattaccaag aagattccgc agcacttgta gacatgaatc ttgtaatgac
    17461 agaatctgga aaattcgtgg agattcaagg aactggagaa gaagcaacgt ttgatggtga
    17521 acagttgaat gaaatgctct tttttggaaa aaacgcgatt gaagatttga tcaaagagca
    17581 aaaacatgct ttgctaacag agtttgctca aaatgatgag cggatagaag aaacaaaaac
    17641 aatcatcatt gcgacaagaa atccaggaaa agcagaagag tttcgaaata tgttcaaaga
    17701 agcgggatat catgtaaaaa cattacttga ctatcctgaa ttgccagatg tggaagaaac
    17761 tggcagcaca tttgaagaaa atgcccgatt aaaagcagaa acgattgcac aattgcttga
    17821 tcagccagtc ttagcagatg attcaggttt gaaagtggat gctttaggcg gtatgccagg
    17881 aatctattct gcacgttttg caggggaaca aaaaagtgat gcggggaata acgccaaatt
    17941 attatatgaa ttgacagatg tgccggatga aaaaagaaca gcacaattcc attgtacgtt
    18001 agtattcgcg gcacccaaaa aagacagcct tgtagtagaa gcggaatggc ccggtcgagt
    18061 agcccgtata ccatctggcg aaaatggctt tgggtatgat cctttattta tcccagaagg
    18121 taagaaacaa acagcagcag aattatcgag cgaagaaaaa aacaagatca gtcatcgggc
    18181 tcaagcaatg aaaaaattga gtgccgagtg gaaacaatgg ctggaaggag agagatagat
    18241 gcggtattta gtagtaagtg ataatcatgg agacagagat atcctcgttg acttagtaga
    18301 tcgttatcgt tctgaagtgg atatcttttt ccattgtggt gattcggaat tagaagcttc
    18361 tgacccatta tgggatacat tccaagtggt tcaaggtaat tgtgattatg gaccaggatt
    18421 cgaacagaaa aaagtgatcc aaactggtca ggatacagta ttcatgactc acggtcattt
    18481 atcgaatgtc cgatttggtt tgacacagtt agcaatcgaa gctcgagctg caactgccga
    18541 tatggtgttt tttggtcata cccatcaaat aggctgtgag atggatcaga aaatcctgtt
    18601 tttgaatcct ggtagcattt ctcagcctag aggcccgatc caaatcccat catatgctct
    18661 tatcgaaagt ttagatgacc aaatttctgt tcaatactat aaccgtgccc atcaggcagt
    18721 tgaagagttg gccttttctt tttcgagaga gcgctgaata aaaaaactct aatttttaat
    18781 aaaaataagc tttattttat gaattttgca tccggtttcg gtaaaatagt agtgttgaat
    18841 gaaatagaaa tggaggccat cagatgattg gacctatagt aagagaaatg ctgctggaaa
    18901 atcaggaaac gtttttgatt cctgcagaaa atgtggcaaa tgttatgtat catcatccat
    18961 tgtcccatgg tttacttgtg ttatcgaagg tcggttattc caaaattcct gtattaggaa
    19021 aagatgatca ctttgtagga ctagtaagtt tatcagatgt ggtcaataaa atgattgact
    19081 tgcaaacaat cagcatggac ccgttagaag gactaacggt tgctgatgtg acagaaacag
    19141 atgttccgac aattgatgaa aattgggaac tagaagaagt actccatttg ttagtcgatt
    19201 cgcctttttt accggttgtc acagaagatc ggattttcaa aggaatcatt acaagaaaag
    19261 agctgcttaa agccgtaaac ttcatggtgc atgaattaga acgtcgtaat atattgtctc
    19321 cgaaaatcga agaattaaaa gaacaaatcg atatcgtgag ctaaagaacg agaaactaaa
    19381 agataatcaa aaaataagta gaaaaaacag cgtaccagtt ttgactttat cttgaaaaag
    19441 aggctgggac aaaagcgttt aactccaaga aataagaagg aattcacgaa aattgctttt
    19501 caaatttttg tgaatttcag cttatttccg aaggagttgc ttctgcttcc gccgtttata
    19561 cgtgtttaga gcgtgggaca aaagtgttct ttacttttgt cccacgctct ttttagtgag
    19621 attcgttgca tcgtttgtcc aaattatttt ccgacagcaa ttggactgtt cggaatcgtg
    19681 aaaccagaag ctgtcaattc tttgacatag gagctcaaga actcttcttt taaggcatat
    19741 tgttttccgt tcaatgcgta gcagaccgta cgaatcgcaa aattgctgtt tccaagatcc
    19801 actaatccaa agatagaagg ttcagtttgg agctcttctt ggtatttttc agctaatgta
    19861 cgatttactc gatcaatgat ctcatagatt ttgtcatatc cttcttcagg aacaatccga
    19921 atatctaaaa gtacttgcat atttgcacga gagagattgc tgatcgtggt gatgtttcga
    19981 ttcgggatga aatggacagt accatccgtt gctttcagct gaagggtacg gataccaaca
    20041 gatacgacgg ttccttcaat cgttaagttt gctagcttga tgtagtcccc aacatccatc
    20101 tgttgttcca tgataataaa aaatccggta ataacatcac tcatgaaccc ttgggcacca
    20161 agaccgattg ccacacctgc aattccggca ccagccaaaa gtgatccgat cggcacaccg
    20221 atagcagata aaattgcata gataaaaaag aaaccaaggg tataatgaaa aatagtattt
    20281 aaaagactat gtaaagtctt taagcggctc tcgtttagaa tggctttttt agaatactga
    20341 ttaaaagact tatcaatcag atattttccc acacggtgaa gaatccaaaa aaggacgatc
    20401 aaaaaaatca gatataaact tttttggata aaggcagcca aaatggcgtc ccaatcaatc
    20461 ccgtcccaaa accgctgaaa cgcatttact ttgctgacag cttgatctgt caatgtctcg
    20521 ccagaagagt ctgtcgtacc ggcagttgct aataatgaaa acataaaatc ctcttttcta
    20581 aaatttgatt ctttctgtat tgtaccaaga agttttcaaa aaaggaaata gcaaaaataa
    20641 ctataatgaa ggaaaaaagc tgaaaataaa atgacaaata atatggataa tgataaaatg
    20701 tggagaaaat aacgaaggtg gtaagtatat gcaaatcgat tggaacaatc taggtttttc
    20761 ttatatcaaa actccttggc gttttattgc caaatggaaa gacggacaat gggaagaagg
    20821 agaattgaca gaagataatt acctgacgat tcatgaaggc tctccagcgc ttcattatgg
    20881 tcagcaatgt tttgaaggtc taaaagctta tcgtcgaaaa gacggcaagg tcaatctctt
    20941 tcgacctgaa caaaatagtc gacggatgaa tcaaagtgct agacgattgc tgatgccaat
    21001 ggtccctgaa aagatgttcg ttgatgctgt caagcaagtg gtaaaagcaa atgaagattt
    21061 tgtgccgccc tatggaacag gcgctacatt atacttgcgc cctttactga ttggggtcgg
    21121 tgaaaacatt ggtgtccatc cagctccaga atatttattt gtcgtcttct gtacgccagt
    21181 aggagcgtat ttcaaaggtg gactaaaacc aacgaatttt attgtatctg attacgatcg
    21241 tgctgctcct aaggggactg gtgcagcaaa agtcggcgga aattacgcag cgagtttact
    21301 tccaggaaca gaagcaaaag aactggatta ctcagattgt gtttatcttg atccagccac
    21361 acatacgaaa atcgaagaag taggtgcagc aaatttcttt ggaattacaa aagacggaaa
    21421 attcatcact ccgcaatcgc cctctatctt accaagtatc acgaaatatt ccttgttgca
    21481 gttagctgaa gaacgactag gactgcaagc aattgaagga gatattttca ttgatgatct
    21541 agatcaattt gcagaagctg gtgcatgtgg tacagcggct gttatttctc caatcggtgg
    21601 gatttatcac aataacaatc ttcatgtatt ttatagtgaa acggaagtag gaccagtaac
    21661 aaaacggttg tatgatgaat tgacaggtat tcaatttgga gacatagaag caccagaagg
    21721 ctggattgaa gtcgtggaat aatttcaaag taatgagttg aaaaaaacgt aaaaagaaaa
    21781 aatc
//
LOCUS       DACDIH010000041        21225 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDIH010000041 DACDIH010000000
VERSION     DACDIH010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.041"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1154..1552)
                     /locus_tag="IV281_001676"
     CDS             complement(1154..1552)
                     /locus_tag="IV281_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780667.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIKFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1768..2823
                     /locus_tag="IV281_001677"
     CDS             1768..2823
                     /locus_tag="IV281_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAP8780668.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQDI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDESLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2843..3196
                     /locus_tag="IV281_001678"
     CDS             2843..3196
                     /locus_tag="IV281_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780669.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQAHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(3378..3674)
                     /locus_tag="IV281_001679"
     CDS             complement(3378..3674)
                     /locus_tag="IV281_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780670.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3678..4496)
                     /locus_tag="IV281_001680"
     CDS             complement(3678..4496)
                     /locus_tag="IV281_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8780671.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4722..4943
                     /locus_tag="IV281_001681"
     CDS             4722..4943
                     /locus_tag="IV281_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAP8780672.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            4945..5211
                     /locus_tag="IV281_001682"
     CDS             4945..5211
                     /locus_tag="IV281_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8780673.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            5324..5863
                     /locus_tag="IV281_001683"
     CDS             5324..5863
                     /locus_tag="IV281_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780674.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGDYSSANEVIEEKKGYAKNGLE
                     IVGTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKLQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVT"
     gene            6072..6254
                     /locus_tag="IV281_001684"
     CDS             6072..6254
                     /locus_tag="IV281_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HAP8780675.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            6357..6641
                     /locus_tag="IV281_001685"
     CDS             6357..6641
                     /locus_tag="IV281_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780676.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            6657..6893
                     /locus_tag="IV281_001686"
     CDS             6657..6893
                     /locus_tag="IV281_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780677.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            6915..7169
                     /locus_tag="IV281_001687"
     CDS             6915..7169
                     /locus_tag="IV281_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780678.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            7251..7436
                     /locus_tag="IV281_001688"
     CDS             7251..7436
                     /locus_tag="IV281_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAP8780679.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            7503..7898
                     /locus_tag="IV281_001689"
     CDS             7503..7898
                     /locus_tag="IV281_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP8780680.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            7888..8265
                     /locus_tag="IV281_001690"
     CDS             7888..8265
                     /locus_tag="IV281_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780681.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            8266..8661
                     /locus_tag="IV281_001691"
     CDS             8266..8661
                     /locus_tag="IV281_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780682.1"
                     /translation="MKQEKRKMKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLW
                     KYKILFLFWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
     gene            complement(8968..9366)
                     /locus_tag="IV281_001692"
     CDS             complement(8968..9366)
                     /locus_tag="IV281_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP8780683.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(9558..10325)
                     /locus_tag="IV281_001693"
     CDS             complement(9558..10325)
                     /locus_tag="IV281_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8780684.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            complement(10360..11583)
                     /locus_tag="IV281_001694"
     CDS             complement(10360..11583)
                     /locus_tag="IV281_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP8780685.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            12563..13366
                     /locus_tag="IV281_001695"
     CDS             12563..13366
                     /locus_tag="IV281_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8780686.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            13951..14406
                     /locus_tag="IV281_001696"
     CDS             13951..14406
                     /locus_tag="IV281_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780687.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            14525..15583
                     /locus_tag="IV281_001697"
     CDS             14525..15583
                     /locus_tag="IV281_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAP8780688.1"
                     /translation="MKKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            15625..17574
                     /locus_tag="IV281_001698"
     CDS             15625..17574
                     /locus_tag="IV281_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP8780689.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            17577..21218
                     /locus_tag="IV281_001699"
     CDS             17577..21218
                     /locus_tag="IV281_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780690.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
BASE COUNT         7428 a         3338 c         3732 g         6727 t
ORIGIN      
        1 acttttgttt cgtgttaagt ttctttacat tcctacgcac cttgcgtgta aaatttcttt
       61 attcctccca cgctccatgt tattttatat ttcctttttc atgttatttt tatccagaaa
      121 ccgaacaagt ttttacctat tcccaaaatt aatttattcg ttattttttc attgattaag
      181 aataattatt tgatgcttaa attagctaaa aaaacgattt tttactgata taatcatgat
      241 attaaagctg aattaggcgg aaagataaaa tgttcgcaaa atcaaagtat caattatctt
      301 gtttttttat aaatttgtaa gtaaaaatct aaaaagtttt cttttaattg gtatatgtat
      361 taaaatcaca ttcaatcact cataaaagta tgcgctttca tttttttgga tgagattaat
      421 atcaataaat aacgatgtaa aaaccattaa ttatgctctt ttataaggat attaattaat
      481 ctaatttgaa aaaataattc aaaatttaaa tgttttaata ttacaaataa aatcatgtta
      541 tcgtaaaatg gtataaaaaa gctaaatatt ctatttttgt tattttatta gcagggaaat
      601 ttaaatagtt tacaagaatc cgtagaaata tattggtttt gccttcagat taatactgga
      661 tcatattaat ctgaatataa tatatgaact aataaatagc ttaacctcgc tattttgttt
      721 tttctgatca ctatctatac agcattagta aatagaattt taaaaataaa aaaattaaga
      781 caaaaaaagt atcaacataa acatatacat atacacgttt atgttgatac acttaatttg
      841 tcctttaaaa agcacttatt ttaatattat gttattttat aatcttatta caagctgatt
      901 gttagacgtt gtcctttcat tacatttatt aacggttatt ttatcgttta atttattaat
      961 tatttttgat ctaaaactta gcttaacaaa taaataatta accaaccgcc agataaaaat
     1021 gcgatcattg ctaatacata agcaaagata aaaatatgtt cgatcagctt aaaccctgat
     1081 gcgattaagc agaccaagta aaatagaaat tctcttacag ataatggtaa ttttctaact
     1141 agcatactca taattaacac cccaaagtaa tattgtattt tgaacatttt acatccaata
     1201 cagcatcaat caaccattgc caatcaaaaa tacaaggctt cattgaagta tgtttttcag
     1261 atgaaaagat ataatataat ttcttcggaa taatattttc agttgttggg atcaagctaa
     1321 atttaatacc atctaactca tagctaccag ctttctctcc attaaaaatt tccttagcac
     1381 tttcttctaa agaatcaaaa tatttttcga cagcaacctt tgaagctttt actattgaag
     1441 catttccctt ttcgataaaa tctgcttttg ttttaccgac aataagtaat tcttgatcag
     1501 taaaatcttc taaaataata gtagtgtaat tcttatttct tctcaatcgc atcaggatca
     1561 ctccttaata taatttatgt ttttataata tcataataag ataaatttgc aagtgtttat
     1621 ttacaccttt ttacttacac tttattgtaa ttgattatac ttttttgatt gcattttttt
     1681 cttataaaca gttgcaacta ttagtaaata ggtgtaaact attttcgtac cgaaatacca
     1741 taaacacaaa cagaaagagg tcataccatg aaagaaccat tattgttttc cgttgactta
     1801 gggaataaat cagtaaaaga aatgagtaca cacgtttctc cactctcgct tccctctcgt
     1861 attttgaaca aaaatatcat taattcagga cgtttttcta atataggttc ctcattaaaa
     1921 cgatcactaa acgtttctga atatagccta gtaaacacta ctgaaagcta ttattttggg
     1981 aaggatatac acgagctagg aaaagatgac tacgtgatag aatcactagg ttttgggatc
     2041 agcagataca agcaggaaac ctaccaagat atcctatctt tctctttagc taacctagca
     2101 cgtttagaag atcaatctga tgacgtttta accgttaatc tagttttagg tctgccttca
     2161 tccgactata ccgaaacaat cgtcaaatac gtgatgcaac aagccacacg acaacattca
     2221 gttgaagttg acggtaaagc ttataatata aacgtagata ccgtttgggt actgccacag
     2281 ccgattggaa cactatataa cctcctcctt gctgatgatg gtagtgtaca agatgaatca
     2341 ttgatcagtc agaagatcgg ttttgttgat attggtggtg gcacgaaact ttttgatgtt
     2401 gtccaaaatt ttgaaattga cgaaaacaac cgtgaacaaa aggataccgg tatttttact
     2461 ttatataatc gtatcgtaaa tatgcttgac cttccgatca gtcctaatgc tttccaactt
     2521 gaaaagatta tccgagaagg caaagaaaaa ggcgtatatc tatttaaacc aagcggaaat
     2581 gaaagttttg atatttcaga agtagttgaa agagaaataa aaacatacac caatatggtt
     2641 gttgcagaag ttttagctac attcaaaaac ttcacaacct tagataaagt tgtctttact
     2701 ggtggtgggg cttctattat cgacaaacaa cttgtaaaag ataaaattcc gcataccttg
     2761 tttgttgaag acagtgaatt ttctaatgta aaaggctttt acaagtttgg tttaactcaa
     2821 taaaacagcg aaggagatca acatgaataa gggaaaatca gagcgttatg tagttataat
     2881 caatagagaa aaggttagcg agttaccttt agcagagttt cttgaatctg aatatgaaac
     2941 actaggcgtt aaatactctc agacgatcaa aaaagctttg aatctatatc ggcgtgtaaa
     3001 gaacaatgaa gttacaatta ttgaagcagg caacgataga aacgatagca aagtagctga
     3061 agtaaagaat aaaccaactg atcataaccc tattcaacaa gcgcatcctt caacggttac
     3121 atccgatcat tcagcaaaga aagaaaagcc aatgctaaaa gggttcaatg caccaagtaa
     3181 caaagactta ctttaaaaat aaaaaggcga ggttacaata cctagccttt ttattatgta
     3241 caaatacaaa cataaacata tacgtataca tgtatataaa catgtatacg tatatatcca
     3301 taaacacgta tacgaaattt agttaagcta tacctaaccg cttattccaa taaaaaaagt
     3361 gatcagcttt aaactgatca ctcaccaaaa acttcatcat acaagttatc tatcatttca
     3421 ccaagtaaag ctgaatctga tcgaattcct aacttttcgc tgattttaga tagtttcttt
     3481 ctttgtgatg gttgaagcgt gaattgataa tttttcttct tttcagaact atcacttttt
     3541 agtttagcta ccaaagaact tgttgtttct ttaggtgttt gtttaggttc ttcaactgct
     3601 tcaaccactt caggaacttc ttctacttca gcttcagcga attttttaaa tctattttct
     3661 ttcacttcaa tcactcctta ttcttgtgcc gtatcaatta ttttgttgta tagcgtaaac
     3721 gtgctttcaa agaaatcctt attcttacga tagatattct tatccttagc gaactcagca
     3781 atattcttac cttctaagct agttgcatta agcaattcct tctcttgtac atatccaaga
     3841 taattagata acgttggtac caattctagg aactcacgac ttgaattagt attatgtttg
     3901 atcctgctac cgatcgtagc gtatttagtt tccatcagtg tttcaccagt cttaatatca
     3961 atcaactgtt ttgctagtgc atcaatatct ttaccaagct taagcaaagc ttcaaaacca
     4021 tctttagatg gatcagaaat gcctacgaca agatcagata cagcccaagc attttgagct
     4081 agtagacctg catcattatg agtatcaatc agaattagat catacttctc tttaagctgt
     4141 tcatagttaa caacgatcca tctaaacagt agggttctgt tgttaggcat atcacgcatt
     4201 tgcatctcaa cttgttttaa atcttcacct gctactaaaa tatccatgtt ctcatttata
     4261 gctaaaggtt cagctgatcc atgaagatat aaatgattga ttgtattctc tcttttcgct
     4321 ttactgaaag catcaggaaa tcgttttgat aggttttgcg acttatcttc atcaatatac
     4381 agcaccttca tacctaaatt ttcgacagca tatccaccta gattgtagat tgttgttgtt
     4441 ttacctgttc cacctttttc aatgtttcca gtaatgattg acaacttctt ttccatgatt
     4501 aaacctccct tattaatctt ttgtaactcc attatacact ataaacacgt ttatgtatat
     4561 atttatatac ataaacgtgt ttatatttat atttgctttt atattctaca acataaacat
     4621 atatatgtat atgtttatgt ttatgtatat attcaaatat ataactattg catttgtcat
     4681 acaatcgtag tacaatgtaa ttgaaaagga gttgataaac tatgccagta ataagtattc
     4741 gtgtatctaa agaagaaaaa gattttcttg aaaaagttgc tgaattcaac ggagataaca
     4801 tttctgaatt tatgcgtagt aattccctgc aaacagcaga aacgttagta gactttgcca
     4861 cctacaagca actaatgaaa gagcatgagg aaaacgatca aagtatttcc cacgaagata
     4921 tgctaaaaga gttaggctta taagatgact taccaagtaa gatatgaacg tgaagctcaa
     4981 aaagcgctaa aaaaaatgga taagtttcag gcaaaggtta tcctaaactg gatcgagaaa
     5041 aatcttgtag gcactgatga tccaagaaag catggtaaag gtctaacagc taataaatca
     5101 ggctactggc gatatagggt aggtgcatac cgtataatag ctgatattag cgagcatgaa
     5161 gtgacaattc ttatattgtc tattggtcac agaagtgaag tttataagta acagacaagc
     5221 tgataaggct tgtctgtttt tttgttaacc caacaaaatc actgtttacg atcccacttt
     5281 acctaaaaaa gaacaatatc ctcttgatat tatacacacc agtgtgtata atataagtgt
     5341 aaacaagcta attaactttc aaaaggagcg attttttatg ggatggtatg gtgattattc
     5401 atcagcaaat gaagtgatcg aagaaaagaa agggtacgcc aaaaatggtt tggaaatcgt
     5461 aggtaccaaa gtaacgaaga cctatggaat tatccttttc aaagatgcag aaggaaccta
     5521 cgatattgac agcttcatct ttcgagatga aatgtacaaa ccaatctact tcttaaatga
     5581 cagtcctgct tactttaaga aaattccgaa aaaatggttg aaattctttg tcggtgatca
     5641 agaaaaaatg gttaagctac aactagatag aattaaagaa gaaagcaata aaccaaaact
     5701 tgatgactta ttagttgcag gtaaaactta ctcaatctgg gatggatcgt atgtcgctgt
     5761 ctatcagtat aaactcaaac gtagctttat ttttcgacta caagatggat cactcactcg
     5821 gttcactcga ctatcagcag gagatgttaa ggaagtaacc taaaacttaa atacataaac
     5881 ataaacgtgt atacgtttat gtttatgtat atgtatatga atataaacgt attaggtacg
     5941 aacactttta aattcttttc tgggctttgt ttttagggta gggctaaaaa aaacatcacc
     6001 atctgatcca aaacagctca gaaacgcatt taaacgtatc tgataccaat attacagaag
     6061 gagtgtatcg tatgattaac ctatttcttt tagtgacagc tagtacatta ttctttactg
     6121 gaatatcact gataatttcc tatttgatct ataagaaagc cgaaaatcac acaagcaaag
     6181 caatcggact agccgtttca tcctttttaa ctttgctgac tttatttgtt actttatcgt
     6241 tttcttttgt ttagaacagc tgatccatta gcaccttttt ctctttacat tatacacact
     6301 gttgtgtata atgtatttgt aaacaagcta attaactttt aggaaggagt gacaagatgc
     6361 taacagctac tttacagcaa cctactcacg ttgctattgc agaaagttat acaattaatg
     6421 cagggatcgt taaacagctg atcaaggatc gagaattcaa aaagtttaac agtcggcaag
     6481 cgtataacag acagctaaag aaagctattc taaagcacaa agattccttc atcgggaact
     6541 tggaaagtgt agatgttaaa actgtttcaa cggcattttc agttgttgaa gtgactattg
     6601 acggtcgctt gatttctcta tttgcttttg gaattaattg atagggagtg tgaactttgg
     6661 aaggtaagac ttttgcagaa atttggactg aatttattga ggtaccaact cgcattgatc
     6721 cgataacttc tgaaaaggta ttggatgcag acttctacaa tctcaaagca ggtactagcc
     6781 ttgaatgcgt ttggatgtat ttagaagagc atttcaaagg cgtaacgctc ggtcacgtct
     6841 tataccacaa tggtgccgag tgggatgcaa ttggaaatat ccatttttct taatataaga
     6901 aaggagaaca caatatgaat tataccgatg aagaacaaat cgctttggaa tacgttttca
     6961 caaatgaaga aggggaggaa attgaaaaag tcttgatcat ttcatcaaaa gacttggaga
     7021 aggctttggc ttatgaacca tacgaatcaa ccgtaactgt cattgaattc ttagatacct
     7081 atacttctaa tgatacttgg gaaattatga atatttgcaa cgctaataac attcaatttg
     7141 ctgaaaaaac caataaatca ttttattagt tttgcaataa aacatcctct ttttctattg
     7201 attccataca caacaatgtg tataatagat tttgtaaagg gagggtagct atgcctaaga
     7261 cagcaagaga aattgtaaaa attctcaaat ctaacggctt caaaaagact ggacaaactg
     7321 gcagtcatgc aaaattttat aattccaaga ctaaaagaac ggtcgttgtt ccgatccaca
     7381 atggtgatgt accactagga acagaagcta atatctggaa gcaagcaggg ctttaacagc
     7441 cttgccttcc tatttggtat taggcatact atcctctctt acactagaaa aggagcgagg
     7501 aaatgaagac aaaaaatatt ttttatccgg caatcttcga gaaagaagga gaagcttata
     7561 acatcgtttt ccctgactta ccggaaatat ctacctttgg aaatagtctt gaagaagcat
     7621 atttaaacgc taaggatgct ttaggattag cgctatatag tgtaccagat gcagaatttc
     7681 ctaaaccatc agcagtggaa gaattaactt tgaaagccaa ccaagtggta gtaatcatcc
     7741 aactaaacat taaacttttc cgaagaagct taaatacaaa aacaattaga aaaaatgtta
     7801 gtgttccgga atggttagtt cttttaggga aggagaaaaa cataaacttc tctcaactac
     7861 ttcaaaaagc actggaagaa gagttgcttg aaaagtaaaa aggagaccga acctatgaaa
     7921 gctacacact taaactattt ctctcgaacg ttaatcaata atctattaaa agatacaact
     7981 ggcattagcg ttagtaccaa acactttgcc aatgatctaa ttatttttga ggataataac
     8041 ttgcgaattg agatgtttat tgaacaggca acccctaact caatacctga attctgctat
     8101 acgcacactg aaaaaatgaa acatgcaaaa tcagttaaag atgttgaagc attaagtgaa
     8161 tcactaacga acgctttaaa cctcgttaag aaggcaaaat tgttatttga atttcaaacc
     8221 gactaccaca atgagttgct tgctcaatct gaaattttag attaattgaa acaggagaag
     8281 agaaagatga aacaaacaca aacagaaacc gttaacgata cgcttgctga tcaattagca
     8341 ggttattata aattatttga gatgggtaaa gcacagcgct tgatccacta tctttggaaa
     8401 tacaaaattt tgtttctttt ttgggaatca acactaacat tctcagttct tttttataag
     8461 tcagattttt ataatcaaca cttagtttta gggagttttt tgcttatcta caccattttg
     8521 atgcttaatt tatataccta catcacactt gaagaacact tagtaattag cgatgcaggg
     8581 atcgagagaa acatcaaaag ggtatccttc aagatcacgt taaacaagcc taccgataaa
     8641 aagctctcag aagcgttctg agagcttttg acattttctt gtataatttt atcctaacac
     8701 attacaaagc acctaattcg agcttatatc aagcaaattt gctatttatt actcaataca
     8761 tatctaaaaa ttacttgatg gaaaattaga tacatggtaa gcatgaaaag aaaatcaatt
     8821 tgagcagatg cagtacaata catagcaatt taaaagctct cagaaggctt ctgagagctt
     8881 ttaatatatt ctttgataat ttgtctttct taaatccata cgtcctaaaa caagctgttt
     8941 ttgcttaata tgatcatctg agagacatca aatcaacttt tctctcaaca aatcattcaa
     9001 agctttcgac agattaatat tctcttcagc cgctaaaaca tttaaatagt gttccacagt
     9061 cactgatttt cgatcataat tttgcttgtt tcgagcttta gcaactttcg tattagcttt
     9121 tacgacaaat aaattaacat tctcagatcg cttgattctt gcatttaact cttgaacagg
     9181 cgtaggcgat ggaatttctt tttcttgcaa ctctaaaagc accaagtatc cttccaatac
     9241 ttcttgcgcc atctcaaaag catcttctaa agtttcacca tgtgtatata agtctggaaa
     9301 atctgggaat ttaactgcat attcatttcc tttccattcg aatactgcac aaaaaaagta
     9361 tttcatcgca ttgcacctct ctaattaaaa aataattttt gcttttttgt ttcaaaaaaa
     9421 agaatcgcct accaaaaaaa ttgataggcg atcacaagct aattaacctc taaaggagat
     9481 taataccttg ctcccaaggc actatatatc ttagcatacg tctgaagcat cttcaaacga
     9541 gtaagatata ttttgcatta acctaatttt ttttctctat aagttttttc atcaaactca
     9601 ctagaaatat taaactggac agcaaaattg taatttctgc cttttctaac tttagcaaaa
     9661 tcaatattca gcattgattt ttcattaatt tcctcaactg attcccttaa aacacgtcta
     9721 tcaaaattcg atattgtttt atactcattt tctgttttcg taatctcacg aattttaact
     9781 atttcaatca ggggattctt atatatttca gctttctcaa aatctccttt atttttggcg
     9841 ataagatatt ttttataata catcgccaaa tatttgtata acacgatgct atattttttt
     9901 tgaaaagatg ctaagtcttc aaaaacaaag aacggatact tctttttgat tgagacaatt
     9961 aagggaatta catcttctga aaaactgaaa gatacactat gctcttctaa atcccaatga
    10021 atccgatcaa tagcagatac agtagtatac gtcttatatt ttttgtctag attcgaaatt
    10081 tcataaactg cttgtctttg caaagtgtta actatggttt ttagttccgt ttcaagattt
    10141 tgatcaacat ccatgtaatt aataatcgct ttaacaggta aatctactac gcctgttttc
    10201 tttagctttg tgggtgagat ataactgaac agcaattgat aaagttcata cgactttaaa
    10261 tccattttca tgtcaattgt tgctaagaac tcctcattgt actttgtcag ttcatctttt
    10321 ttcaaaagtt tttccttcct tcacgcattt tttcagttgt taatcataaa ggttcacata
    10381 aggcaaataa ctcgtaattg atttttcgag atatttaaca gtatttttta ttgtgtaatc
    10441 ctccttatga gtagcaacgt aagataggtg attgacaact gattcaaatc ctctatcttt
    10501 ggataaacga tcataaagcg gataaacttt attctgaagt cggctcataa gtttaagatc
    10561 gcataagtcg gcagaactga tcaaaaagtt tttgattagt aatttagtgt atttgctatt
    10621 catagctttc aagtacgatt gattcatttt ctctgtttta tcttctgcgc cttttaaata
    10681 agcagggtct ctttgttctt gcttataagg taccggaact gacatggtac gtgtaagcaa
    10741 gaactttatg tttgtataag tatgaccctt cttgataagc tcatagtcaa ttttaaaatc
    10801 agtgtattta ttcagttggg caatcggttc tttaattaca cgtctttcaa agtctcgaaa
    10861 agctttatat tttttctcaa tactaaattt ctttcgtaaa tctgcaatgg agaaaacagg
    10921 attttttaat ctatcaagtg attttgtact ccatgttaag ttatctctat ttgcttcata
    10981 cagattaaaa tctttagtga ataattgaaa aaaactgatc gcatacttgc ttgtcaagtt
    11041 aactacttga tcaaatgtat attgcgtaaa atgatcttct aagttcgtta ataaaggcat
    11101 aattgaacta ctaaatttaa tagatactgc atcagaaaaa tcattgaatt cagtaatttc
    11161 tagcggtgag atgactttgt attcagtctt accgttaggt ttagttcttt gaattttaat
    11221 gacagattgc tgatgcaatt tttcaataac ttgttttaat cttgaatgtt ttgaagaaga
    11281 tgaactagaa gacatcaacg caaataaagc ctttttagat actttgacgg tattattaaa
    11341 aggcggattt ttagggtcaa gatgtcctat agcgtaaaga taaatttttt gtggcatagc
    11401 atccatttca acaatgctat ctacaagttg attcgataaa gtaacagtta aatcaaactt
    11461 ttttagattg ctttcggtag tttcaaattt cgggtttaca ttcttttctg ttattttcat
    11521 acgattgcct cttctttcta agcttggtac gagcttaaaa acttggaact ggtacgattc
    11581 cattgaataa gtatagacct gttttcgata cgttacaata agaaatgtat cgtaaattta
    11641 taacataact caatgtcagt aaaacacatt ttattttttc caatcaaatt tcattgaaaa
    11701 tacagcagaa atcgctattt tagaacattt tcaaaataaa aatccttttc aataaaatac
    11761 gaaattataa aaattaccaa aattaaaaaa caaatatttt tctagttttt agaaataaat
    11821 tcacatactt tttctttttt tggatatatt cggaatacca catcgctttt tgtccacgtt
    11881 tgcatcgctt tttgtccaca ttcacatcgc tttttgtcca tgttcgcatc gctttatgtc
    11941 caccctaaaa tctatttaag attgatataa agcctttttg gaggttgcta aagttttaaa
    12001 gattaaaata tatattataa atataaataa aaggggaagc tgaagcttcc tccttttcct
    12061 ttttttcaat tattcaaagt tacacaagca aaataaactc ctagatagtg ttagtttttt
    12121 taagattttt tatttttaag attttgaaat aagagaggag taatcctcct ttattaaata
    12181 tctgttaata tatctgttaa atatctgtta atatatctgt taggggaggc ttaatctctt
    12241 gggagagtaa gtattaaagg ggtataaagg taacactttt ggacagtaaa ggtaacaaaa
    12301 gtggacagta agggtaacag ttttggacag caaaggtaac aaaagtggac atttgaggta
    12361 tcacactagg ataagacgtt accttcggtt ttcttgattt ggaaggcgaa aaaatagaaa
    12421 attagattag taacaaatct ttgttgactg ttacctttag ttgtgattta atttaggtaa
    12481 caagcagaaa aaattgtttc ctttaattag taccagatta acatgattaa aggagcgcaa
    12541 aaagaagatt ggagagtaat caatgtctca agaagttacc gaaaagatta ttgatagtga
    12601 tgtagattta gtatcagaac aaacaaatgc aattgtgcgt tataacaatg gtctgaatac
    12661 aacagctttg cgaggattta agcctgttga aatggatgtg ttttgggcta ttttatcaaa
    12721 aatgaaacgt caaggaacag cagaagtaac ttttgatttc aacctctttc gttatttggt
    12781 caaatacgac agaagagaaa aggacagttt ttattctgtg ttaaaagatt tgagtgacaa
    12841 actaggtaat ttaacctacc gttttgaaga ttcagacgtc tatgaacagc tgtggctttt
    12901 tcaaaaattc cggatagata aacagaagga aacagttaca attaaggcaa atgaggaatt
    12961 tgaatttatc ttaaactcaa ttggcaaaaa ctttactcgt tttgaacttg ataattttac
    13021 aaaactaaga agtagctaca caaaagaatt gtatcgtcat ctcatgcaat acagaaataa
    13081 agagtggcga aatggttatt gggtagtcaa agttgaagat tttagaagag cattaagtat
    13141 accttcaagt tatagaatga caaatattga taaaagaata ctagagcagg cgaaagaaga
    13201 atttttagct cctgatgaaa acggaataca ggtatttgaa acttttgatt atgaaaaaat
    13261 ctatgcaaag aaaggaagaa gagttgatcg gctcagattc acttttagtg agccagaatc
    13321 aaacttgcca accatttcaa tgcataattg gttagaagaa gattgagaag aagcaggttg
    13381 taattttaat acaacctgct tctttaaaaa agtatgggta acacttttgg acaataaatg
    13441 caagaagttt ttttgtaagc aagtttcagt ctaaataata tttgaaagaa tggctaatct
    13501 atacaaattt taacaactat gggtaacact tttggacaag tcaagaaaaa aaacaatttt
    13561 tcttaatttg ttgatcgact aaaggtaaca cttttggaca acacagaata aaaagtccgg
    13621 tgatatcagt tttggtagga aacatcttaa tatctttgtg agattgaagg taacattttt
    13681 ggacaagtca actatttgtt aaaagttaat atagagtaat tagataagtt ttttcttttc
    13741 gtctgataaa tggataatat tagttatttt attgctaatt agtctttcat gatgactgaa
    13801 atagctaaaa taaatatctc gttggataat tgctgatact ttaagggata tctaaatttg
    13861 ctgattgatt tatttatgtt attatgatat tataataaca taaacgtgtt acgcccgaac
    13921 ctcattttaa aaaaatgaag gggtgagaca atgaatgcta ttaattggca tgaatttgtt
    13981 aactatatgg agagcttact agatgatcta aaaagtttta gtattgctga tgcagtaaag
    14041 catgggttac cagttagtac aacgatgaaa ttacgaggat tgaaaattcc agatgaaatt
    14101 attaagctga gaactaaaac aattattgaa accgcaaaag tcgttgacaa tatgaaccat
    14161 gaactaaaag gcaagaaaac aacttacgag aaagattttg aacgtttttt aaaatctgtt
    14221 attggaaaat atctaggtaa aaaatctttc agtgaattga gagatgaaga gattgtacct
    14281 actccaatgg ttagcaaaat ccgaaaaaac aaagccgagg ggaattacta tcttggtaca
    14341 atagcaaaaa tcattgatta tttggaaaat aaagagcaaa agaaaggaac gaagaaagga
    14401 agataatttt tgtgttaaat attaatgggg aagaatatac cgaaatgatt caaaatcccg
    14461 aacctagaat taccgaaaaa acgtctgatt gtttgctctt ttatgaaagg gggcagacaa
    14521 actaatgaaa aaaaacgtca attcaacatc taccaaagat caatacagta ttattggagg
    14581 gttgcttggt gataaggata agagaacacg tcacggatta cgcattatgc ttgcaacttt
    14641 tctaggagtg ctttttttat taacaggctt gggtagctac atttatcaat taggaaacta
    14701 taattattat ctcgtgaaca atacaacacc tataggctct tcattggagt ttgctcgatc
    14761 aggagcagag atcactattt cagatgtgtg gacagatcaa aacagagatg taacagtagt
    14821 aaaactcggt tactcgcaaa acgctagaaa gttgttatct acgcaaggaa aaaactacaa
    14881 cttatatatg gcaacgaaag aaaaagatcg tcctaaaaag cttgaaatga gctatgggat
    14941 gttaagtact gatggggatg ctttcttatt cctaaaaggt aaattagatg aaagagctta
    15001 tcaagttttt attgcaaacc aagtatcact aatttctggt gatgatacag ctgacgatga
    15061 taccaaagtc aatgtgaatg aagcagattc agttacaaaa gcactttctc aatattctat
    15121 gaatgatgtt aataatgaag gagtaatttc ttttgggaaa aaagcaaaac ctgttaatac
    15181 tcctgataat atcaatttca gaataaatcc atattcagat tcaactaatg tctatcaagg
    15241 aagctttctt accaaagatg gcgatattga ttattcaaaa gttgtagcag ttacaagtaa
    15301 gaataatgtt gtaagtaagt tagaagatca gttaaatgct tcaaatacta acatcaagaa
    15361 aattgaagct acaaaagctg agtatctgga acgtttacaa caaaatcctg acgataaaga
    15421 ttcgcaagaa agtattcgtc aactagacag tcgaatggag accgaaatta aagcaatcaa
    15481 tgatacacaa aaattgattg atcaatacaa aaatgctgac tttaacaaag attcttttgg
    15541 agacatgaag acgacattta agtacctaac tctgactgac tagaagtaaa ttattcttat
    15601 tttagaggaa gtgaacacaa acagatggaa aatagtaccg agttttttaa gaatattcaa
    15661 actgaattta atcatgctct cacaccaatg aaaccaccga aacataagtt agtgggacgt
    15721 gatgacgaac tccatgagtt gtcactgatc ttgaatagac cggaaacacc gcaagctatc
    15781 ttgttaggtg aagccggaac aggtaaaaca gcacttgcaa gtgaatgggc tgaaagacag
    15841 cgacaagatg gaaagaaagt acaagtatac aaattatcaa tcggacaatt gcgtgttggg
    15901 ggagatgatg tcctccaaac acgtttagaa acattaattc caagattgaa agaatatgag
    15961 cgaattttaa aatctgagga caaagaaaat gaatttgtcc tcttcattga cgagtttcat
    16021 aaagttgtta gctcttttgg tagtggcaat aaaattgggg gtgattcgct aaaagaaccg
    16081 ttagcaaatg aatcacttca attgattacc gctactacaa gaaaagaata tgaaaactat
    16141 atcaaaagag atgatgcctt agggagacgg ttacaaccgt tagaattgaa tgaactaaaa
    16201 agcgatgttg tcgtcgatat tttgagacaa tggctaaata catcctctgt taaagatgat
    16261 cctcaggctt cttttaaatt atcgcaaagg attcctgatg aattacttca agaaatcgta
    16321 caagcaaaca agctttatcg tgaggatttg tccgaaccgg ctaaatcaat tgccgtaatg
    16381 gaggatttga ttgcaaaaga tcgagtagac ggaaatgctt ataatcgaga aagtctattg
    16441 caaatatttg gtaagtataa tattcagcta gattctgatt ttgatatgga aaaacttcaa
    16501 gaggaattaa agtccataaa agggcaaccg ttagcagttt atactataac aatgttgttt
    16561 aaacgtatga agcttggatt tgatcgtttt aaaggtcgaa agcaaccaaa agcaactgct
    16621 ctgtttacag gatcaacagg ggtcggtaaa actgcaatgg ctaaagccgt ggcaagagga
    16681 ctttatggcg atgaaaataa agttctctta gttaatatgc cggactacaa aacaaaagat
    16741 gatgcaatgc gttttagacg actcataggt actcatgtaa ggcatgatcc ttctgcgatt
    16801 attgtgtgtg atgaatatga gaaagctggt gtagaaacac tcgatgcctt tttatcaatt
    16861 ttagatgaag gtaggataag ttttactgaa agaggatggg atgatcgaga agttcacgac
    16921 actgtttcat tgtcaaatac aattttattt gcgacaacaa atgcaggtgc ggagatgttc
    16981 gattcgttaa acagatatag ctcactatct cgtgttacaa acaaggaaaa ttctaatgaa
    17041 tatagcactg aactaaaaag agaataccga aaagtcgcat cagggatcaa acaagccttg
    17101 attggtgaag gtctaaagcc tgaagttatt aaccgatttg gacgaatagt tccattttgg
    17161 gctttatccg aacaaactct tctggaaatt gcttataatg aaatgttgaa gagtgctgaa
    17221 ttagtacgaa aacatttaaa tatagattta cgtcttccac ctaggaaaga aagagtatat
    17281 ggtcaaaatt tttatgcaga cgatctctcc atgcacttag tttttgacat ggcttcacaa
    17341 gaggattcta aatcaggggg tgcaaggaat atcttgcatt tagttgaatc tgagtttgaa
    17401 gaagcgatta ctgaagcaat aattacttat ccaaacgcaa aaatattcga tgttaagttg
    17461 agtgaagccg gagcgtttga aggaaaaggt gattctaatg ctgaaattat tgcaaagcct
    17521 tccgttgctg aaagtaaaga aaacaagatt gtattttcga ttggaggggc gtagctatga
    17581 aattcaagaa atatttacta caaaacctta gaataaaacg atgggtcgta agaggtctag
    17641 ttattggagg agtattagca gttactttag gcgctccttt tggaatgaat gatccaactc
    17701 ctgacaatca agatagcaaa caagaaacag ttataaataa gaacgttgca tatgcggaaa
    17761 gtaaaccggc agaacaagga atgtggacac tatatgctcg tatcctaatg ggagataaag
    17821 aagttaatga tagtgccgct aaaactggac aaatgaattt gttaggacaa ggtggtatca
    17881 gtgccgattt cccttattct gacatttcta aacaaggtaa ttcgattgca gggggtaatg
    17941 caggtactca actagcatct actttagcaa cttatagtta ctacggatat attgaaacag
    18001 tctcaggaaa tgctattgcc agtcaagcat catccattct aggaagtatt ggtaggacga
    18061 ttggtagttt tatcggatca attagtttaa tcctttctaa tattgctgaa tccataaatg
    18121 aactccttgg aaaatttttc atgagtatga atatattttc tcttatgggg tggggagaag
    18181 gtggatcgga tgtatctaat ccggtatcta aagcattaac tagcttgctt aacaatttgg
    18241 gattaaataa tgaattattt aaagcgatag caagcttatc tatgctcttt ttcacagcaa
    18301 tactaggtta tcaaggaatg aaattattgt cgaaagatgg tgttaactcg aagggattat
    18361 caccagtact tgaaaaatgg attgttcgta tttttgtatt gtttatattc ttaccagtgg
    18421 caggatcgct attgggagtt tatatcaacg gcatccagtc agcaaaaaaa gatggagata
    18481 taccaacaaa tgttgtttct gactacatca ttaacacaag actttgggct tctactcaaa
    18541 atcttgctcc atcatctgat actggtggtg atgtacctga cggaaagcct acatccgaaa
    18601 tcaagttcgt tgatacagat tatgaaccat ctaaaaaatc tgcaatgata agagcgatca
    18661 atactagcag ttatgccaga caaaataatg atctagattc taattcagtc ggtatggctc
    18721 tattaaaaga atggtcatcg aatgatactt tcaatattaa tacttatgcc ggtgatgtta
    18781 atgtcgccga aaatgatgct tatatgggaa gagcgcctgc tgtttggaca ggctataatt
    18841 taggggatgg aaaagaagga agtgcaatta agaaaccaac caatttaaat ggatatattt
    18901 ggtcagcaaa tcaaaatgtg acagacgaca atcgcaagcc tgatggaaaa ggaacagcgc
    18961 ataactttga tgctactcaa ccttggggcg ttgctaacaa tgcaactttt agcacacagt
    19021 cggtagcttt attgctccaa tcggcacttg atacatcggg agctaagttt tatgcgtata
    19081 atatcgctcc ttcaggaatt caaggcagtg ctaagaatat gtcaacagta aaaacagaat
    19141 ggcgtacagc atccttagtt ggtcatggag ctatgggtaa gtttggctcg tggctagcga
    19201 tgaacgctga atcaatcgtt ttagcagttc tttacttagc atgtgcttgg gcgatgttca
    19261 atgtgaatat cgtgaaagca tttaaacgtg aatttattcg gatttgggaa gtagttctaa
    19321 caggatcact tgcatcagga gcaggtgtga tggcaatttc attaggtgtt gttacaacat
    19381 ctctgatcgg actacaatta cctatctttc tgattacttt tattcgtgca tttggtgata
    19441 ctgctgtggg attcattcct aatacattag ttggcgatgg cgtagcaaat attgttcgat
    19501 ctgctatgat tttaggtgta ggatatcttt tagtctttaa aaaaggcgaa aaagtaaata
    19561 atttgattaa tatgcttatt ggcttaccac ttgaagtagc gtttgggttt gatgaacgtg
    19621 ctaagttatt atttggtaat agaaatgatg ctgtgcgagt tgtggacggc gtttctaaag
    19681 caatcaagaa aagccaagga gctaaaaatc cctattatga tggtggcaac agctcagtaa
    19741 aagatatggt tgcaggaggt cttggagcag ttggagcgca aaaaatggct gaacgtttga
    19801 acaatatggg agatagcaat gctgatgcta agtccattgc tgataaagta attcctgaaa
    19861 caccagaagg aatgtcattc aacggtggcg gagttggttc cacaatcaat aatacctctt
    19921 tcaaagattt agttcgtcaa gatcaactac gtaataactc ccaacaatat attgatcatt
    19981 taggacgtat ggttgatgaa aacggaaact ttgtcgatga aaatggtgaa atattaaaag
    20041 atagtaaata cggtaaagat gctaaaggca actttattga cgaaaaaggg cgtttggtcg
    20101 atgaagatgg aaaccttata gatactaatg gtaaaccatt ggaagatagt gaagatggta
    20161 atattggtat ggatagcgaa ggacgtttaa ttgatgaaaa aggtcgctta gtcgatgaag
    20221 atggaaatct tgttgacgaa aatggacgca ggttgctaaa tggtgatttt ggtcatgata
    20281 gtaacggacg tttagttgac gaaaaaggtc gtttagttga tgaagatgga aatcttgttg
    20341 atgagtttgg taataaactt ggtaaacaca gcgatggata taatgtgaat ggagatttag
    20401 tcgatgagga tgggaatatc attgagacga gagatgcgct tgtggcagat cgaaatagag
    20461 aactcgaaca acaacggcat acgccaattg ctgttgcaat tcctgagaat gaagaagacg
    20521 agattacttt agatcaaact catcaagaac tgaggaatga aatgagtggt gaggacgtgt
    20581 cagacagagt gcatcgggaa ttaggagatg gagcatctgg tgaaagtcag tcaagagagc
    20641 ttaacagcaa ctttgataat agtgctattc ggaatgacac agtacttaaa gacagagaat
    20701 cgctaacaaa agaaacggat aaagaacaat cagatgtgaa gaaaaagttc attcctactc
    20761 ctaatgttaa tcaaacagat aaatatgaaa caaatattgg atcaggaatg tcgaaagaca
    20821 atcaatcaat tagagaacct gaactgacaa ataaaaataa tagaacaaat aattctaaat
    20881 ctacttcaga taattcaggg aaatctaatc aatcaaccaa gaaaacatca acagctaaat
    20941 cagccgttga atatctatca aaaactggta aaaatgttta cacctcagca gataaagctt
    21001 tagcaaaagc tccaaaaggt agtctagcag ggataaccta tgaagctaaa aacgaagcta
    21061 ttaaaatagg taaaggaacg gctaaggttg ccaaggctac tgctacagga gcatccaaaa
    21121 ctggtaaagt agtgaagaaa tctaccatgt cagtcaagga ctatattgta aaatacggta
    21181 atcaaaaatc aactgaaaat aagtctgaag acgagtaaaa agaag
//
LOCUS       DACDIH010000042        20904 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDIH010000042 DACDIH010000000
VERSION     DACDIH010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.042"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..306
                     /locus_tag="IV281_001700"
     CDS             <1..306
                     /locus_tag="IV281_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8780691.1"
                     /translation="QSLDIQSLKNSYPKDLSGGERQRVAIARALYNEPSLILADEPTA
                     SLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDGYLTEEEKKKE"
     gene            314..961
                     /locus_tag="IV281_001701"
     CDS             314..961
                     /locus_tag="IV281_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8780692.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(1034..2425)
                     /locus_tag="IV281_001702"
     CDS             complement(1034..2425)
                     /locus_tag="IV281_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8780693.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            2682..6071
                     /locus_tag="IV281_001703"
     CDS             2682..6071
                     /locus_tag="IV281_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP8780694.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            6074..7495
                     /locus_tag="IV281_001704"
     CDS             6074..7495
                     /locus_tag="IV281_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8780695.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            7492..9369
                     /locus_tag="IV281_001705"
     CDS             7492..9369
                     /locus_tag="IV281_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8780696.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            9461..10303
                     /locus_tag="IV281_001706"
     CDS             9461..10303
                     /locus_tag="IV281_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8780697.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(10455..11330)
                     /locus_tag="IV281_001707"
     CDS             complement(10455..11330)
                     /locus_tag="IV281_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8780698.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            11537..12568
                     /locus_tag="IV281_001708"
     CDS             11537..12568
                     /locus_tag="IV281_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP8780699.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            12657..12962
                     /locus_tag="IV281_001709"
     CDS             12657..12962
                     /locus_tag="IV281_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP8780700.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            13141..15156
                     /locus_tag="IV281_001710"
     CDS             13141..15156
                     /locus_tag="IV281_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8780701.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            15158..15442
                     /locus_tag="IV281_001711"
     CDS             15158..15442
                     /locus_tag="IV281_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8780702.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            15468..16841
                     /locus_tag="IV281_001712"
     CDS             15468..16841
                     /locus_tag="IV281_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8780703.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(16924..18939)
                     /locus_tag="IV281_001713"
     CDS             complement(16924..18939)
                     /locus_tag="IV281_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP8780704.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(18961..20175)
                     /locus_tag="IV281_001714"
     CDS             complement(18961..20175)
                     /locus_tag="IV281_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8780705.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            20396..>20904
                     /locus_tag="IV281_001715"
     CDS             20396..>20904
                     /locus_tag="IV281_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8780706.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENML"
BASE COUNT         7103 a         3873 c         3953 g         5975 t
ORIGIN      
        1 caatctttag atatccagtc acttaaaaat agttatccta aagacctttc tggtggagaa
       61 agacagagag tggcaattgc tcgcgcttta tataatgagc caagcttgat cttagcagac
      121 gaaccaacag ctagtttgga tacagatcat gcatatgaag tcgtcaaatt actagttaaa
      181 gaagctcatg agaaacaaaa agcgactatt atggttactc atgatgtccg tatgattcaa
      241 tggagtgatc gcgtttatcg aatggaagat gggtacttga cagaagaaga gaaaaagaag
      301 gaatgaatcc ttcatgacaa tgaagggcta tccacgtata atcagagata aaggagagat
      361 gtggatgcca aaagaaactt ttttcaattt atcgaacgca aagaaacaga gaatcgatga
      421 cctattactg gagatatttt gctcacagca catcagccaa gtcaaagtct cagaaatcgt
      481 tgacaagatg gggatgtcgc gcggtgcttt ttataaatat tttcaagatt tggaagatgc
      541 ttatttgtat acgatcaaaa gcagttctca catagtccat caagatatct taaaattcat
      601 tagcgaaaac aaacaacatt tctttttggg gatcgaaaaa tatttagagt ggtgctgcga
      661 cttagatcac gaaagtatct attggcaaag gttatacttg ttaacccagc gaaatgatca
      721 gcagacaacc aaacgtagag aactatcggc tgattctcca atgaacgaaa aatggctgga
      781 attattaaga atcaatcatt tctccattca atcgtcggaa gaagcagtta gtttcctata
      841 cttttccatg accctcgtaa tgaacacttt gacggatctc attgcaaata attggacaaa
      901 agaagagttg ttaaaagact ttcgctataa atctcgttgg attgctgaag gaatacaata
      961 aaaagaaaca gctatgtgta tttgtcctga aatttttatc agggtggttt atacatagct
     1021 gttttttgat tattcataac tcgaataagc ggatgagttc ttcttcattt gcgaatgaca
     1081 cactatcatc aatcaatata tgaggaacat gggcattcaa aatgggttga ttcgtaacga
     1141 tcagatcata ctcgagaaga ttgtatttcg acagctgatc ttcagtaata gcagttgtct
     1201 cagagtgaat tttatgcaca gcaagaatat cactgatata ttcttcaata tattgactat
     1261 gtcgaagact taagtcgctg ataatcagta tttttttatc agaagtataa tggtgaattt
     1321 caggattacc gataagcatc caaaagatat agctagataa ataataaatc agattctgat
     1381 tcagtagagt ggaataaaat tgaagttctg cacgaaatcc agcatataga cgtatctggc
     1441 ggtgttgcat ctgctgtgca aagtaagttg cacggatcgt caaatgagtc gtatcgtatg
     1501 ggaaaatacg ggagaaaaaa taaatttgcg ttaataattg aacaacatat tcttcgtttt
     1561 tttctttgca atcagcggat gaaaacgaac agtaggacat attttttcgt atctgctcac
     1621 tgatttcatt tttttctttc tccgaaaatt ctgaaaaaat cgtattgaca aaatagttca
     1681 ctacttttat atagctgctg tacttcaatt ttcctaagag atttttcaag attaagaagt
     1741 cttttttatc gatatttatc aactgtacat aagagtctcc agctttccat tcaaatggag
     1801 ttccttggga ttctctcatg agatgcacaa caaaatacat aataaagaaa gattgctcat
     1861 atgggctttc ttcctgaaac aaattcatct gacaattatt cttgaccata atttctttga
     1921 tcttcttttt ttcgattgga aatggcaacg tgcgtcttcc gtagtaactt aaaaagaaaa
     1981 aggtaacaaa gatacgcaac tggagctcac ttttaaggct agttaactga aaaccttggt
     2041 caacgatcac ttttaataaa tagctttcta aaatttgatt gcattgtttc aacttacgtg
     2101 taaaagaaga ccggctgatg gaaatttttt cacaataata ctcaatcgtt ttacccggat
     2161 tgacaataat ctcatttaat aaacgactgt ttgtagaact ttgcgacatt tccatcagaa
     2221 catttaaata agctggtaca ttcaaatgtt caagacgata ccccaatttt tgtgaagatt
     2281 gaatcgttac atccgttccc cagcggattt tgatctcttc gatatacctt acaaatgtag
     2341 attctgaact accaattcgt ttggcaagtt cttttttagt catccacttg tcagaaatgg
     2401 acagaattcg aatgattttt tccatctttc tatagtttca ttccaacaac ctgatcatat
     2461 gtttttctcc ctcaaccctt ttctttcaat gaaacgctca ttaatcgaag gaatcaactt
     2521 cataaatgaa accaattggt atgaaaattg gtgtgattcc ctttagcgcc cttcgtataa
     2581 taattatcat attaaaataa ataaaattat gtaacgcctg tttattttaa taattttcac
     2641 tattcacgtc aaaacagaaa aaaagaaaaa ggaggggaaa aatgacgacc acagggaaga
     2701 aactgaaagt tattttcatg ctgataatat tgagtttatc aaactttgtg ccattatctg
     2761 caatagcaga cactacagat gatccaacag ttttagaaac aatttcagct gaagtcattt
     2821 cggatcagtc tggaaaaaaa gcactgaaca tcaagctaaa tgcgaataac accagtgctg
     2881 aaaagataga aaaagaaatt ggtctagtcg aaaattactt aagtgatgtg gaaagaaaag
     2941 aaggagatgg ctatgcttat caggtaaata gcgggaaaat tacgttggaa atctcatcaa
     3001 acactaaaca aactatcgat ctgagttttc caatcgatcc agcactttac cacagccagg
     3061 caaacaagct gatcgtcgat aataaagaat atgacattat tgatgagaca gaaaataaga
     3121 aagatacaga tgtgtcagta ccaaagccag acgaaataga agaagaatca tcaaaagaaa
     3181 acgaaaattc tgtcagccca tttacattgc ctacattatc cttgccagct gtgagtgtgc
     3241 catctaatca aacgattcct acagaatata caacagatga tcagggcact tatcctaaag
     3301 ccagttggca acctacagga aatacaaatg ttcttgatca tcaaggcaat aaaaacggaa
     3361 caaatcaatg ggatggtata aattcttgga atggagatcc taatgatcgg acccattcgt
     3421 atatcgaata tggaggaacc ggtaatcaag cagactatgc gatacgaaag tatgcaaagg
     3481 aaacaagtac acccggattg tttgatgttt atttgaatgc tcgtggaaat gtacaaaaag
     3541 atatcacgcc tcttgatctc gtattggtcg tagactggtc aggaagtatg aacgacaata
     3601 atcggatcgg tgaagtaaag attggtgtcg atcgttttgt cgatacttta gcagatagcg
     3661 gtatcacaga caaaatcaat atgggatatg tcggctactc aagcgaagga tatagctaca
     3721 gtaacggtgc agtacagatg ggttcatttg attcagtgaa aaatcaagta aaatccatta
     3781 caccttcacg gacaaatggt ggtactttta cacaaaaagc actaagagat gcaggaagca
     3841 tgctatccgt tccaaatgga cataaaaaag tgatcgtttt gctgacggat ggtgtaccaa
     3901 cattttccta taaagtacag cgggtacacg cacaatcaag cagcaattat tacggaactc
     3961 agttttctaa tacgcaagat cggccgggaa atacttctct aatctcaaga atctatgatg
     4021 cacctgacca aaacaatcta tccagaagaa tcgacagtac gtttatcgca accatcggag
     4081 aagcgatggc actcaaagaa cgaggaatcg aaatacatgg tcttggcatc caacttcaaa
     4141 gcgatccggc agctggtctc tcaaaagcag aagtagagtc tcgtatgcga caaatggttt
     4201 catcagatga aaaaggcgat ctttactatg aatcagctga tcatgcaaca gatatctctg
     4261 aatacctagc caaaaaagct gtacagatct cagcaactgt aagcaatgga caaataaatg
     4321 atccaatcgc agaaccattc atttatcagc ctggtacact ttcagtcaag agtgtgggga
     4381 caagtcctac aacggtcact ccatctattt ccatagaagg aaataccatc aagagcaatc
     4441 agatctattt aggaaaagac caagaaatcc aaatccatta ccaagtgaga atccaaacag
     4501 aaaatgagga cttccatcca aatttctggt atcaaatgaa cggcaggaca actttccagc
     4561 caaacattga taccaatgaa ttagctgaat tcggtatacc atctgctaaa gctcccggag
     4621 tcagtcttca catcaaaaag ttatgggaag aatttgacaa caatctagct gatcgtccag
     4681 atcaagttac ttttgagatt caacgggaac atacgacaaa tgctgcagct tggaaaaacg
     4741 gatatattcg aatcattaaa ccagctaaag atacaacaaa tacgtgggaa cgtgcagaca
     4801 ttgacaaatt atctgcaaat agcggagaaa gttatcaaga gatattatca ctacctcaat
     4861 acaataatca aggtcaagca ttcagttacc aaacaatcaa agaattacct gtaccaggat
     4921 acgattctca acaaatagat gcaatgacat ggaaaaatac taaacaattc acaccgttaa
     4981 acttgaaaat aacgaaaaat tcctctacag gtgaaaagga tcttattggc gctgttttca
     5041 aattaacagg agattctatt gatactttac taacagatca tggcgacgga acctattctc
     5101 ttccagaaaa tgtcaaattg caaaaagaaa tgacctatac gctgacagaa acaaaagctc
     5161 cagaagggca tggattaagc aaaaagacta cttgggaaat caagatcgct tctgatggta
     5221 cggtaaccat tgatggaaaa acagtcacta cttccgatga tacgatccag ttgactattg
     5281 aaaatccttt tgttgaagtt cctgtagcag tacgtaagta tgcgatgcaa gggacggaca
     5341 aagagataaa tcttaaagga gcagcatttt ccctacagaa aaaagaagca aatggtactt
     5401 atcagccaat tgacagccaa acaacgaatg aaaaaggtct tgccagtttt gattcactca
     5461 cacctggtaa atatcgagtc gttgaaacag ctggtcctgc cggatatgat acttcgccgg
     5521 gaaattatga attccaaatc gataaatatg gaaaaatcat ttacacggga aaaaataccg
     5581 agatgacaaa taatgtatgg acgctcactc atcaaaatcg actaaaagcg tttgatctaa
     5641 cggtacacaa aaaagaagac aacggacaga cattaaaagg agcaaaattc agactgcagg
     5701 gaccagaaat ggacttagaa tcgccaaaag atggacaaga aacagatacc tttctattcg
     5761 aaaatttaaa acctggaact tatacgctga ccgaaacttt tacaccagaa ggataccaag
     5821 gtctaaaaga gccagttact atagttatac acgaagatgg gtcaattcaa gtggatggac
     5881 aagatcatga atctgttctg tcaccaggag ccaaaaacaa ccagatttct ttagacatca
     5941 cgaatcaggc aaaagtacca ttacctgaaa cgggaggaat tggccgttta ggaatctatc
     6001 tagtagggat gattggttgt gcgttttcta tttggtatct ttttttgaaa aaagaaagag
     6061 ggggcagcta aatatgaaaa aacttggttg gcttagtatg tgtctcttct tgttactatt
     6121 taaaccagct tttactcagg tagcaacaga aacagaaaca gaaatggttc agattacttt
     6181 acacaaattg cttttcccaa acgggcaact gccgaaaaat catccaaatg acggacaaga
     6241 aaaagcttta ttacaaacgt atcgaggatt aaatggtgtc acattccaag tttatgatgt
     6301 cacagattct ttttaccatc tacgggaaaa gggcaaaacg gtagaagaag cacaagcaga
     6361 gatcgcaaaa aacggtgcgt cttccggtat gtttaccgca gaagcaacaa ctacaactct
     6421 taacaacgaa gatggtatcg cttctttttc tctggccgct aaagatcaag aaaaaagaga
     6481 taaagcgtat cttttcattg aatccaaagt accagaagtc gtcaaagaaa aggcagagaa
     6541 tatggtagtt gttcttcctg tacatggaca aaacaatcaa aaactttcaa ctatccattt
     6601 gtatcctaaa aatgaagaaa acgactaccc tgatccacct tttgagaagg tattagaaga
     6661 gcctagaaat gattttacga ttggtgaaaa aatcacttat tccttgcata cgacaattcc
     6721 tgtaaatatc cttgactatc aaaagttcga attgtcagat agtgcggatg aagcattaac
     6781 gtttttacct aatagtttaa cgatttcatc gaatggagaa aagctgacag aaggctttgt
     6841 catacacaag aaacctcacg gatttgatgt tttattttcg atcccttcgt tggaaaaata
     6901 tgctggaaaa aaactgacca tttcttatca gatgcagcta agcagtacag cacaggcgaa
     6961 caaggaaatc aacaacaacg gaacactgga ttttggtttt ggtgtcagta caaagaaagt
     7021 ctctgtatat acagggagta agcaatttgt caaaatcgag acaaataaac cagataaacg
     7081 attagctggc gcagtattcc ttattaaaaa caaagcagga aattacctcc agcaaacagc
     7141 caacggatac aagtggacaa agaacgaatc agatgcgctt cacctgattt ccgataaaaa
     7201 tggcgctttt tcaatttccg ggttgaaaac aggaagttat cgattaaaag agatcgaagc
     7261 accttctggt tatattttaa gtgaaacaga aattccgttt accatttcaa cttttctttc
     7321 tgaggataaa gaggcggaca gtatattgaa agtagtcaat aaaaaagaaa atagccgtcc
     7381 atttcttcca aaaacaaacg aaacgaaaaa tacactttta ggcgttgttg gtatggtatt
     7441 cgcaagcttt gcaatctggt tgtttatcaa aaaaagaaca ggagtgaaaa aatgaaaaat
     7501 cataaaaaaa taaacgttat gttaggagtc cttttcctta ttttaccatt actcacaaac
     7561 agcttcggcg caaaaaaagt gtttgcagag gagacagcag ctcaagtcat ccttcataaa
     7621 aagaaaatga ctgatttacc cgatccttta atccaaaaca gcgggaaaga aatgagcgaa
     7681 ttcgatcaat accaaggatt agccgatatt tcattttcag tttataacgt cactcaagaa
     7741 ttttatgcgc aacgagataa aggagcgtcc gtggatgcag caaaacaagc agtccagtct
     7801 ttgactcctg gtacaccagt tgcttcagga acgacagatg ctgatggaaa tgtcacttta
     7861 tctttaccta aaaaacaaaa tgggaaagat gcagtctaca cgatcaaaga agaaccaaaa
     7921 gacggagtgt cagctgccgc aaacatggtt ttagctttcc ctgtatatga gatgatcaaa
     7981 caagcagatg gctcttataa atacgggaca gaagaactag atactatcca tctctaccct
     8041 aaaaatacag tcggtaatga tggaacgttg aaagttacaa aaatcggtac tgccgaaaac
     8101 gaagcactaa atggagcaga atttattatt tctaaagaag aaggaacacc aagcgtcaaa
     8161 aaatacatcc aaagtgtcac agatggattg tacacttgga caactgatca aaccaaagcc
     8221 aaacatttca ttactggtca ttcttatgac atcggcaaca atgactttgc cgaggcatct
     8281 attgaaaaag gccagttgat cgttaatcat ttagaagttg gaaaatataa tttagaagaa
     8341 gtaaaagctc ctgataatgc ggaaatgatt gaaaagcaaa caatcacgcc ttttgagatc
     8401 ctggcaaata gccaaacacc agtagaaaag accatcaaaa atgatacgtc taaagttgat
     8461 aaaacaacac ctcaattgaa tggaaaagat gtcgcaatcg gtgaaaaaat tcaatatgag
     8521 atttctgtca atatcccatt aggtatcgct gataaagaag gaacgcaaaa caagtacaca
     8581 acattcaaac ttatcgatac tcatgacgct gctttaacat ttgataatga ttcttcagga
     8641 acgtatgctt atgccttata tgatggaaat aaagaaatcg acccagtaaa ttattctgtc
     8701 actgagcaaa cagacggatt cacggtttca gttgatccga attatattcc ttcattaact
     8761 cctggcggta cattgaaatt cgtttactat atgcatttga acgaaaaagc agatccaacc
     8821 aaaggatttt ctaaccaagc aaatgtcgat aacgggcata caaatgatca aacaccaccg
     8881 tcagtcgatg tcgttactgg gggcaaacga tttgttaaag tagatggtga cgttacatca
     8941 gaccaaacac ttgctggagc agaattcgtc gttcgtgatc aagatagtga cacagcgaaa
     9001 tatttatcga tcgacccatc cacaaaagcc gtcagctggg tatcggcgaa agaatcagca
     9061 acggttttta caaccacaag taacggttta atcgatgtga caggtctaaa atatggcacg
     9121 tactatctgg aagaaacgaa agcgccagaa aaatatgttc cattaacaaa ccgtgtagca
     9181 tttactatcg atgaacaatc ttatgtaaca gcaggacagt tgatttctcc tgaaaaaata
     9241 ccaaataaac acaaaggtac acttccttca acaggcggta agggaatcta tgtgtatatc
     9301 ggtgcaggag tagtccttct actgattgct ggactgtact ttgctagacg caagcacagt
     9361 cagatttagt ctttttgcaa gaattggaaa aaggagcagc ggattacgca aatgctcctt
     9421 tttcttttat tatcaaacag tcatcacaag gagaacacaa atgaaaccaa agaaaaggaa
     9481 acttctagat ctgctgatgc ttttcttgct tctctcagga atagggatac tagcttatcc
     9541 atttgttagc gacgcgctga ataattatct ggatcagcag atcatctctc attatcagca
     9601 acaagcggtc aaagaaaatg aagaagtcat ggctaaaatc caagaaaaca tgacaaagaa
     9661 aaaccagcaa ttagcaaaag aaggtggaaa tccaggtgca gatcccttta ctaagaaaaa
     9721 agaacctgcg aaaaaggata gaacctattt tgagaaacat acgatcggta ttttgacgat
     9781 ccctgagatc aatgtccatt tgccaatttt tgatgaaacg aatactgtgc tgctagaaaa
     9841 aggtgcctca ttattggaag gaacgtctta tcctacgggg ggagaaaata cacatgccgt
     9901 tatttccagc catcgaggac tgcctcaagc aaagttgttc acagacttac cgaaactgac
     9961 tttaggtgat tctttctata tcgaaatcaa tggacggaca ctcgcttatc aagtcgatca
    10021 gatcaagacg gtagaaccga ctgatacaaa agatctgaga atcgaaaaag ggcaggattt
    10081 cgtgacactc ctaacttgta caccttatat ggtcaacagt catcgcttac ttgtcagagg
    10141 gcaccgggtt ccttatcatg caaaagaagt tcaaaaagaa gtacagaaag tatccgagaa
    10201 caaacgattt ttcctgtatg gattgttagc tgcatgtata ctgattttcc tccttcttat
    10261 atatcttttt cgtcaatcgt tcaaaataag aaacagaagt tagctattcc tcatttctat
    10321 aatatatcta ttcgaatcgt tccgtaaaca aacaggctga ggtttgaaaa agaaaacttc
    10381 agcctgtttt tatgatgata tcaaagagag cctatcaaac actatctttc ttattactaa
    10441 atgaggatct tttcttaagt tttccgatat tctttaagaa ggcctttttg catcaattct
    10501 tgtttgtttt tcaagttgcg gatattctct tcaaaatttt gagaatatat cactgagaat
    10561 aacgtattta atacgtaatc gaaggcaatt tgagaggaga aagtcgctac tttagcaaaa
    10621 tcatattcct cctgcgatat aacaagacac atatcagcct gtttggccat ttctgaatgc
    10681 tgatttccgg taatcagcaa ggtaggggcc tctacatgct tcaaataggt cattattcgt
    10741 tcataatgat ggatgcgacc gctgtagcta ataaaaatgg cgcaatcctc gctagtcaaa
    10801 ttggctgcat tccaagaaga atcactgtat tcttcagcca tgatcaaaaa cttattcaac
    10861 ttgaccattt tattctggaa tttccttgcc gtgatttgag aatcaccttt tgcaaaaaga
    10921 aagattcgct gtgccttgtt caatacttta ccgatttgat ctaaatcttc atgttcaatc
    10981 tgcaattgtg ctttttttat tgactggatc gataaatccg ccattttctt tgcaatttgc
    11041 tgagtgctat cttcacttga aaaaggaaaa ttaggatcaa tcgtttgatt gaactgagca
    11101 atcaattgat gaacttcccg ccccagctcg aaacggaatt ctttaaatcc gctgaatccc
    11161 atttttttgc ataaacgaat gatagctgaa tgggaagtat acgtagcttt tgctaattct
    11221 tcaatcgtca tgtagactgc agcttcgata ttcctacgga tgtaatcgac aatccgtttc
    11281 tctgtcgtgg taaacaactc ttgttttgtt agtttttctt caataagcat gatctgacct
    11341 ccaaattcta tcttactgat aaacacttgg ttccgctact atctttttga tttttatcct
    11401 tactttgaac attaatttca ccaaaccgaa tgataacaag tgtttttctg aactttttta
    11461 ccagaatagc tattataaat actttctgcg cttttctagg atatactgaa aacatcaata
    11521 aaaggaggca ccactcatgc ttacaatcgc ttatcttggc aatggaaaga gtacaaatcg
    11581 ctatcatttg cctttttcaa gaaaacttac gaacaaaatc aatatcaaaa aaattttttc
    11641 cccatctgaa ccagtgtgga aaacattcga agacatcgaa tatactgatc agctgtctga
    11701 tctttgggat gatcctgaga tccaattagt tgtcatcact acacctagtc aatttcatta
    11761 tgaatatgga aaaatggcac tcgaacatgg taagaatgta ctagtagaga aaccttttgc
    11821 agaaacgtca aaggaagcag aagagttatt tgcattagca aaagaaaaag gtctatttat
    11881 ccaatgttac caaaacagac ggttcgattc tgacttcttg acagtccaaa aagtaatcga
    11941 aagcggaaaa ttaggggata ttttagaagt agaaatgcat tatgattatt tcagaccgga
    12001 agtaccagaa aatacgcatg aattttctac tgcgaacagc tatttatatg gacatgcttg
    12061 tcatacagta gatcaagttc tttcttattt cggcgaacca gatgatatca gctatgatgt
    12121 acgtcaactt ttagggaaag gacgaatgaa tgattacttc gatctggatt ttttctatgg
    12181 aaatatgaaa atttcgataa aatctagtta tttccgcatc aaagaacgtc caagtttcgt
    12241 cgtttacgga aaaaaaggga tgttcgtgaa acaaacaaaa gatcgacaag aagaacatct
    12301 gaaaatgttt tacatgcctg atcatgaaga ctttggtatc gatttgcccg aacattatgg
    12361 tgtattgacc tatatagatg aacaagggag ttatcatgaa gaaaaagtaa tttccgaggt
    12421 tggtgattac agccgtgtct acgaagggat ttacgagaca ttagttaatg gcgcagaaaa
    12481 agtcatcaaa gatgaagaaa caatcttaca aatgaaaatt ttggaagaag gaatcaaaag
    12541 cattcaagaa tcagaaggaa aaatatgaca tatttgtaag aatcgggttt gttttttgac
    12601 gcataaactg tcttattgta cgatgaaaaa gtatagaact actaggagga accaacatga
    12661 caaattatga cggtgaaaaa cacaaacgat taagaggttc aatggcccct aaaacagaag
    12721 aagaagtggc tttagcagga actaattctg gtgaaacaga agacaaagaa gaatcattag
    12781 ctggcacaaa ctctggtgaa acagaaaata aagaaacagc tttatctggt tcaaattctg
    12841 tagaagagga aacaaagcct aatgagccca atcactttga aaaagatatc gaaaaagcgg
    12901 ttgaagatgt caaagaaaaa tttgaagaac tgacctcaaa aaataaacat gatcatcatt
    12961 gatcggacga aatcaattca taaaagcagg agacatttgg tcacctgctt tttttctgta
    13021 tccattcgat agaaatcgtt cggtacagtc gtaaaaaaaa tccaactgta agttgtacca
    13081 aacacccctc gatttaaaag cgctaccata ctagaatata agtaggaggt gaacagcgct
    13141 gtgaactaca aaattagtta tctcctgaaa acaccaagag aaatcagtct agaaaaagca
    13201 gcagagatca tcgaagtaaa tacaagcgag gcgatcaaac aattaactcc ttttgccgaa
    13261 tatgtggatg atacccgtat ccgtataaag aaattccgag caatcgactg ggcagaaaaa
    13321 tttttggata ttactcgatt tgaagctgtg tataccgaac aagagcgaag agatcttctt
    13381 tatttgatgg tttattcgac tgcaacgtct gtttctgttt ttcattttca agattttctt
    13441 caggtttcta aagggacagt cctgtcagat atcaaaaaga taagaaatga attagaagtg
    13501 gaagattgtc agctgtcgta tactaggaaa actggtttta ggctgctagg gtcagaagca
    13561 atattaagaa gagaagcaaa aaatcgcact gctcgtttga tgcagtcact tgctggaaga
    13621 tttgggctca cgtatttaac cgcaaccctt tccattgatt attacgccaa agttagagat
    13681 ttgctagata aaacgatttc actatcagat cttcgattcg caccaaatcg aatggatgaa
    13741 ttagcttatt atttgagttt gacaaccatt cgaattggaa aaacatccat ggaactggta
    13801 aaagatgctg aactgttggc ttctcttgca gtcactgaca ttagtcgaca agtattgact
    13861 ggccttggct attccgaaca tcaaaattcg gagattatat ttttctctct ttgcttagca
    13921 tcgatcgttg aaggagatgt ccaggagcct gcacttgatt ttttgctgga atgttcttca
    13981 gaagtcatcc atcgcatgga attcctgatg gccgtagaat ttgcttcgtt tcgtgaatta
    14041 ttgatgaatg tcttttatca cttggtccct gcctattttc gtatttccta ttctttttat
    14101 ttacctaatg tgatgatcga tcaaattaag catcaatatg cttccatcta cgaaatgaca
    14161 agaaaagcat tacttccgtt agaaagacga atagggaaga gcatccctga agaagagatt
    14221 ggttttttca ctatactatt tggcggagag atcagaaaag cggacgcaga agaaagaaat
    14281 agaaaaataa gagccgtgat cgtctgtcca agtggcatta gctcttcatt gatcttaaaa
    14341 agtgaacttc agcaattatt cccgatgatc ttttttacag agactaattc agctgaccgc
    14401 ttacacgagg ttgacgaaac ttcttacgat attatttttt ctactgtacc tctcacactt
    14461 actgataaga aaaagtctct ctatgttctg catccaatca tgactgactt ggaaaaaaat
    14521 cagctgatca atcaagtaca aagagattgg ctgataccag gattttcaat gccttctgcc
    14581 caagaattgt tgtcggcgct actgccttat atcgaattaa aagaaggcgt agacgaagaa
    14641 aagctctatc aagtattaaa tcgtaaaatg aacaaacttt tagaaaagaa ggaggatcaa
    14701 cgtcctatgc tatctgaatt attaacagaa gaaatgatcc aattatcgga tcaaccaaaa
    14761 aactggcaag aagcaatcac atttgccgca cagccgctat tatcagaaaa taaaatagaa
    14821 gaaagttata tcaaagctat gatcgagcgc gtggagcagt atggtgcgtt tatccatatt
    14881 ggcgatcaca tcgcattacc tcatgctcgt ccagaagatg gcgtaaacga agtagggatg
    14941 tctttgctga aacttcaaca acctgtggat ctgcttgata atccagccca tcccattagt
    15001 ttgttcatct gtttagctgc tatcgataat gaggcacatc ttcgggcttt ggcaaattta
    15061 acaaagctac tttctaataa acaaaatctt caagatttat tacacgcatc aacaaaagaa
    15121 gaaatattag cgatcataag aaaaggagac gaatgatatg aaaatttttg cagtatgtca
    15181 aagtggatta ggaacaagtt ttatggtaga gatgaacatc aaacaagcac ttgcagatct
    15241 tggagcagat gtggacgagt ttgaagtaag tcatacggat gttggaagtg tatctagcga
    15301 tatggctgat tatttcttca ttgagcattc tttgaaagat acgctaactt ctatacctga
    15361 tgaaaagctc gtacctttgc aatcgatcat tgacagcgaa gaagtaaaag aaaaagtaac
    15421 gaagatcttg tcaaaagaat aaaaatgatt caagggagga actatcaatg gataaattgt
    15481 tgaatttgtt tgtgtcaatc gtcagtcagc cctccatcct cgtttctttg attgcactgc
    15541 ttggtctcgt tcttcagaag aagaaatttt cagaagtgat ccaaggaact atcaaaacat
    15601 ttgtcggatt cctagtactg atcggcggtg ctggtttaat cagtaattca ctaacccctt
    15661 ttgcttcgat gttccaaaca gctctgcata ctcaaggagt agttcctagc aatgaagcag
    15721 tagtggcgat tgctttgcaa caatacggta cacctactgc tttgatcatg ctagtaggga
    15781 tgatcgtgaa tattttactt gctagattca caagatataa attcattttt cttacaggtc
    15841 aagcaatgct ttatgtttct tgtctaacag cagtcatttt agtaagcgcg gggtttactg
    15901 caaatttgtg gatgatctta ttaggcgggg tcttcgaagg aacactatta actattacgc
    15961 ctgctctttg tcaaccgttt atgaagaaca tcactggaaa caatcaagtt gcgttagggc
    16021 ataccggaaa tatcggttat gcttctagtg gatggatcgg aaagtttttt ggaaacaaag
    16081 accgttctac agaggacata aagattccta aaagtttcag ctttttgcga gattctactg
    16141 tatcgatcat gctcttgatg tcgatcgtat atgtcgcact tgctttgtta gctggtcctg
    16201 gatttgttga aagagaacta agcaatggag acaacgctgt aatctttgca ctgatccaag
    16261 ctggaacatt cacagctggg tttgttatcg tccttcaagg ggttcgaatg gtgttggcag
    16321 aaatcgttcc agcattccaa gggattgcta aaaaactcgt acccgattct aaaccagcat
    16381 tagatgttcc tatcgttttt ccatatgcac ctaatgctgt attaattggc ttttttgtta
    16441 gcttctttgt aggaacacta tccatgtttg ctatgatagg cttgcaaact acagttatca
    16501 ttcctggggt agtaggacat ttcttctgcg gagctgcttc tggtgtcttc ggcaactcga
    16561 ctggcggtag aagaggggcg gtattaggtt ctgctttcaa tagcttattg attagttggc
    16621 tgccattatt tatccttcca gtattaggag atcttaaaat cgcttcttct acatttgccg
    16681 atactgatta tctagtacca ggacttgtac ttgggaaact aggagaatac ggtcaaatag
    16741 ccttgacagt tggaatcatc gctttctttt tacttgtcgt attatctagc tttctttttg
    16801 ccggaaaaag aacaaacatt gtttctaaag aacagaatta agcaaacttc aaaataaaaa
    16861 ttgcttggac aatatcagaa gaagatttaa agtcttctga tatgtcgcaa gcagtttttt
    16921 gttttatgca tttatttttg aatagcttgt taagacaagt ggctgttttg ctagaagagg
    16981 ataactttta ccattcattt tcactgttcc gcctgttcta tcagaagtaa tcacgatctt
    17041 atcttttgtt ttttcaagcg aaatctttcc atctttgata ggtagcgtac aattcagtgt
    17101 ttcaaatgaa tctagattag gttgtatcat aaattcttca taacctggct ttgttggata
    17161 caaaccgata aaatagcgtc ctaaaagata gattgggctt gctccccatg catggcataa
    17221 gctttttcca taaggatctc catacatact gtacttttca tttcctgttt gagtagggtc
    17281 gtattcttcc cagaaagtta ccgcatcatg agataacatc cctccccaat aatcaagaat
    17341 cgtttggtag acgatattcg tctgtccgag tttgcataaa gcatcttgtt catagaattt
    17401 gaaatatgga gtagtaatct gtgtgatagt gtcattcaat aagacattct ttaagatcga
    17461 ttgctgttgg tctttgttag caaaatcgaa taaaacagcg aaaatattcg catgacgcgt
    17521 cacatttctt ctcccagatt catagcaatc gataaaagcg ccaagttctt catcccaaaa
    17581 gtagtcaaat aggttttgtt tcaatagttg atatttttct tgatagagat gagtatgata
    17641 attttcgttc tctccatatg tttcagctaa gatatctccg caagtgatca tggaatgata
    17701 ggctttcagt aataagactt gttctgcagc aagcgttcct tctttatcca tttcagacca
    17761 atcaataaat atccaatctt ttgctctccc ataaataaac cctaattcat tcgtttgctc
    17821 catcaagtat ctcattaatg attgcatctt agggtagacg atgcgtaaaa attcatagtc
    17881 tttcgtcatc atatagtggt tttcgatacc aatcaaccac aagatcgaat agtcaacgat
    17941 agtattcata tgctgcttga tttcattttg tcctcttaat gctaatatcg ttcgtttatt
    18001 gatttcctcg tcaaaaaaaa gatattgatt gatcatgaat gattgatacg catctccaga
    18061 ccaaatccag cgatctcgtt tgataccgtc gataaaaaac aaatcactac ataacttgaa
    18121 ggcttcttct gatactgacc agatctgatt cagtaaagaa gaatcgcttg aaaaagcagc
    18181 ttgtttcgaa agagataaaa attcatgaaa aacttgaagt tgaaccattc cagtagaaac
    18241 ctctggtata aacaaatagc gaaaagcgcg tttacgagct ttcatttttc ttgtcattcc
    18301 tatttgctta tagtagcaat aatcgatatc catagcttct acatcggact ctccataaca
    18361 tagagtgata gattcgatat ggtcatccag agatattagt tctacacagc cattcactgc
    18421 tcgcccaaaa tcgaataaga ttcctccgcc aatctccgtt tcagtgactg gatggacgca
    18481 ttctttttga taatagattt gatttggatc ttttgcgata tcgacataaa gcggactcca
    18541 gccagctgga tattcttcaa taaaattact tgctgtccag ccaatatctg attttacttc
    18601 gtctccctcg ataaacatcg ctggtagccc tgatgtatgt cctacaaaga tacgaatttt
    18661 tgctttacca gctggacaag tcagccattt tccacatgga tgtttttgtc cattaatttc
    18721 tacataccca actccttgga tcgtcacttt aaacatggaa ggttgatcaa gaaagtaata
    18781 tctcttaaac ttgacgttcc gatggcaatc atccatgtac cagtacgctg gccagtcaaa
    18841 acctcgttct tcccgttgga aattttgtag cagtccatgg cggatctcaa agtctccagg
    18901 ataccaaagc catctatctt tatgtttttc atttgtcatt ttacacgacc tccatcaaat
    18961 ttattttgtt gtcaattgtt cttttatttc ttcatctact cttatcactg tctttctatt
    19021 cgtcctgtgt actactagaa ggctaataag cgcgctaatc aacagcaaga tactgccatt
    19081 gacgaaaaca gcaagtgttt tactcccaag agaagcaaaa gagagaaaga gcaaaccata
    19141 tggtgcaatg aaagtaccta aattagcgcc aaccaataag attgaagtat tgaaactctc
    19201 cgcgtacttg ggcgcttgga tacttacttg gttgaataag taaggaatga ataacgaaaa
    19261 agaaattcct gttaggatcg caccaaaccc aatcacatac aggtttatca cagtggcaat
    19321 caatacaaag ccgattccca tcagtagtaa agaaagtgta agcatatagc gatgtgcttt
    19381 tgtctgaagc cagccaaaac aaactccggc agccatactt gccaattgca tgattgttaa
    19441 gatattcgct gcattagttt cgtttcctag agaataagtc gtcaggactg tggctaattt
    19501 aatcgataca gcattatata ccacgatgac cagcaagaga aaaataccat aactaatcat
    19561 tttaggattc gttttgatta cttgtcttgt tggggctgtg ccatgcgatt gaagttcagt
    19621 tgtttcaggt gtaggaacaa acaaaacaaa caagacaaga ataggtaagc tgatggcgta
    19681 gactaaaaag gtatattgcc aaccgaactg tactagctgt cctgcaacaa atgtcattaa
    19741 agctgcacca aatccttgaa atgcactttg aaaaccaatc atagaggctc gttcatctcc
    19801 gtgataaaag gcagcaatca tgctgactgc caaagaattg atcaatccaa agccaatacc
    19861 tagtccagct cttgaagcta agacaatact gtaactttgt gagaagaggg ggaggatacc
    19921 acaaatcgat gcaatcacta atccgatgat cacagtttgt ttttgtccga ttttcctagc
    19981 aataaaacta ctaatcaaaa caaaaatagt gaccataaaa gagggagcag tcgttaataa
    20041 ttcaacagca gataaagaac gcgtggcata agtagctgac atactgggaa ttgcagacgt
    20101 gactgcacca gcagaagtca aaataagaga gactgacagc agagaaaatt tcatcatctt
    20161 tttattcata agcatgttcc tttcattttt gtaagcgctt atcaaaaata tagtatgaag
    20221 tggttattct ctcaatagcg gaatcacagg aagttcatgt cccaaccata gaaagtcttt
    20281 atgaaaccaa aaatagcttt gcatataata gcagtacagc gtgaactaat aagcgaaaga
    20341 aaccgcttac aaaaaggagg gagtcaattg gaactagctg aattagacat tttttttgcg
    20401 taacgaagaa tcaatcgaaa ctctgcagcg tgaacaagga atgaatatca atgatttaaa
    20461 cttagcttac catcttcaga caccgccaaa aatccaccga ggggatctat ttcaacaggg
    20521 aaatattcat atcagtaaac accgtcgata tgcggaagtt cccgagcata cacatgattt
    20581 tgttgaaatc aattatatgt atagcggtac atgtttacaa attattaatg gagaaaggat
    20641 agagatggaa gaaagtgacg tggtgatcat tgataaagaa gctactcaaa gaatcgaata
    20701 cacagggcaa gaggatattt taatcaacat cttattaaaa gaagaaacct tatctacaga
    20761 gatattgaat catttggcaa gcacaaataa tctactttct tcttttttga taaatgcatc
    20821 aaaagaaaaa ggggaacata cacaattcat tcattttcgc ggcggaaaaa caagtcgttt
    20881 gacgcatttg attgaaaata tgct
//
LOCUS       DACDIH010000043        20749 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDIH010000043 DACDIH010000000
VERSION     DACDIH010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.043"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(80..1216)
                     /locus_tag="IV281_001716"
     CDS             complement(80..1216)
                     /locus_tag="IV281_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8780707.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPNLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKMPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVDFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPKGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(1333..2025)
                     /locus_tag="IV281_001717"
     CDS             complement(1333..2025)
                     /locus_tag="IV281_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780708.1"
                     /translation="MKKVILGLVFGAFFLTGCSQSNVVDNTGKTNEKDSSVTLSESTT
                     QSSKNSQEVDGPLLKVGQWTKEKRDTSGKIELIGTASPQSDIPLGDIGIYISNIKLLK
                     YSNYADPSAAGYYDANNHKDSEGNFYGLQIAFKVKNNSEVDYGYNGLAHAILDNGQQI
                     DFSSDDLMHTMTTNSFFKKTESKEFYSLAYLDPSKVNDITKVTIKTGVLYNPSDYSTV
                     AESAESTFNINR"
     gene            complement(2163..2816)
                     /locus_tag="IV281_001718"
     CDS             complement(2163..2816)
                     /locus_tag="IV281_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780709.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKVSKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQDTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     GQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(2866..3294)
                     /locus_tag="IV281_001719"
     CDS             complement(2866..3294)
                     /locus_tag="IV281_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8780710.1"
                     /translation="MEMDTINLVEELKRKYRSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIDELKNLYGLPESAYRFLI"
     gene            complement(3299..3673)
                     /locus_tag="IV281_001720"
     CDS             complement(3299..3673)
                     /locus_tag="IV281_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8780711.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            3958..4143
                     /locus_tag="IV281_001721"
     CDS             3958..4143
                     /locus_tag="IV281_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8780712.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALVGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            4156..4404
                     /locus_tag="IV281_001722"
     CDS             4156..4404
                     /locus_tag="IV281_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780713.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKITPKEFIEKHLVDL"
     gene            complement(4703..5368)
                     /locus_tag="IV281_001723"
     CDS             complement(4703..5368)
                     /locus_tag="IV281_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780714.1"
                     /translation="MSLIDFIKQFDWNNIVKTIKDFILPITSLVFSTFAFYISYTDRK
                     NKKFNLKLDFFAECEEWLIDRESDSKPDVYHQNKFRIIDSVLLTNNSSLPVTIIEFSI
                     SGIPDHLNAFTMIGDNYSVTVKSMYDELPHGIRAYSGKSLKKGADLSKFPPLSLPITI
                     PPYESKITTLVFRYDESLVEKNITINVLTSRGTAKFNRFVSSSQISQLDTGYAPPQLD
                     EFD"
     gene            5423..6121
                     /locus_tag="IV281_001724"
     CDS             5423..6121
                     /locus_tag="IV281_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP8780715.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFSEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLKEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNIKVMEVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDENE"
     gene            6299..6640
                     /locus_tag="IV281_001725"
     CDS             6299..6640
                     /locus_tag="IV281_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780716.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            6633..7304
                     /locus_tag="IV281_001726"
     CDS             6633..7304
                     /locus_tag="IV281_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP8780717.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            7310..7996
                     /locus_tag="IV281_001727"
     CDS             7310..7996
                     /locus_tag="IV281_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780718.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVTNFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            7999..8226
                     /locus_tag="IV281_001728"
     CDS             7999..8226
                     /locus_tag="IV281_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780719.1"
                     /translation="MINLNAVAFEQTWRTKYKKMSPRDKLFLEIMTFAFIGTQAEQSD
                     ISVEKIKTNRLVNGITETCYQYTIIVVDEEE"
     gene            8229..9056
                     /locus_tag="IV281_001729"
     CDS             8229..9056
                     /locus_tag="IV281_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replisome organizer"
                     /protein_id="HAP8780720.1"
                     /translation="MAERRMFAKTIIDSDAFLDMPLSTQALYFHLSMRADDDGFINNP
                     KKIQRMVGCGDDDLKLLMAKRFILVFESGVIVIKHWKIHNYIRNDRYKPTLYQDEKAL
                     LADKDNKAYTFAEELSKHDEKLGIPDDNQAVYQMDTQVRLGKDRLGKDSKEIKDLTPS
                     KKSKAKPIRHKYGEYKNVLLSDEQMEKLKTEFPNDYQERIERLSEYCESSGKTYKNYL
                     ATIRSWARKEKSESKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            9068..9337
                     /locus_tag="IV281_001730"
     CDS             9068..9337
                     /locus_tag="IV281_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780721.1"
                     /translation="MKIDYLELINEIANYKTGEEIDILRDVYDQLEEAGIEGINNDRS
                     SWSKLRYYFALYIDATQLRNLAYTKLLFVDCIKGLQKHLSELEQV"
     gene            9499..9801
                     /locus_tag="IV281_001731"
     CDS             9499..9801
                     /locus_tag="IV281_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780722.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVTEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEKK"
     gene            9798..10109
                     /locus_tag="IV281_001732"
     CDS             9798..10109
                     /locus_tag="IV281_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780723.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
     gene            10106..10324
                     /locus_tag="IV281_001733"
     CDS             10106..10324
                     /locus_tag="IV281_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HAP8780724.1"
                     /translation="MIGLTIFWCAIGGLLAFLGYYLICESRKGRKYTVTGIVLLIIGI
                     LVAVGAELNLYVNGTQEDLVRFMFWMKN"
     gene            10335..11150
                     /locus_tag="IV281_001734"
     CDS             10335..11150
                     /locus_tag="IV281_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAP8780725.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            11289..11645
                     /locus_tag="IV281_001735"
     CDS             11289..11645
                     /locus_tag="IV281_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP8780726.1"
                     /translation="MDLITQYSDIILKKIMAKIQKDKKAKERAELVKLEMAETGVGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKAHSIQHGFI"
     gene            11605..11850
                     /locus_tag="IV281_001736"
     CDS             11605..11850
                     /locus_tag="IV281_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780727.1"
                     /translation="MIKKLIQFSMDLYDIESGATVSVESDHLIISFADERQIIIWVVD
                     DMLYPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSK"
     gene            11847..12245
                     /locus_tag="IV281_001737"
     CDS             11847..12245
                     /locus_tag="IV281_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780728.1"
                     /translation="MIPKFRAWDKRENTMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVEIFEGDVVITSKGAIGYVAYLQQEAGFVVVLKKSDYRLG
                     HRNTGESYDVATAHEVIGNIYENSELLEVE"
     gene            12242..12580
                     /locus_tag="IV281_001738"
     CDS             12242..12580
                     /locus_tag="IV281_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780729.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            12577..12807
                     /locus_tag="IV281_001739"
     CDS             12577..12807
                     /locus_tag="IV281_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780730.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            12814..13017
                     /locus_tag="IV281_001740"
     CDS             12814..13017
                     /locus_tag="IV281_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAP8780731.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASETIRKFGISILNDDRKWEAER"
     gene            13014..13307
                     /locus_tag="IV281_001741"
     CDS             13014..13307
                     /locus_tag="IV281_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780732.1"
                     /translation="MKLKDGFYTDAYGIGGLMMDLPTKNPIKQEESEIKVGDMVRCEA
                     EGFIYPFRGYVEHLYNHSAIIRIENTMECDKRLAKSKENLAVVRLVDMEVIKS"
     gene            13337..13777
                     /locus_tag="IV281_001742"
     CDS             13337..13777
                     /locus_tag="IV281_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8780733.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            13852..14319
                     /locus_tag="IV281_001743"
     CDS             13852..14319
                     /locus_tag="IV281_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAP8780734.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            14596..14832
                     /locus_tag="IV281_001744"
     CDS             14596..14832
                     /locus_tag="IV281_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780735.1"
                     /translation="MYWRVYKMNNASEKIDQLTILVNQRIEDIIKEFEGEKIKSIKLT
                     LMDHKFELNSTKRKIVELIEKEIAVQINEQQLTN"
     gene            14856..15068
                     /locus_tag="IV281_001745"
     CDS             14856..15068
                     /locus_tag="IV281_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780736.1"
                     /translation="MDKESARELYLSKFKRNNKPEEVFGSNVKEVGEKLTQLLKEEDL
                     TYDEAYASLQYCYNLLKYESNFLKLQ"
     gene            complement(15046..15519)
                     /locus_tag="IV281_001746"
     CDS             complement(15046..15519)
                     /locus_tag="IV281_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780737.1"
                     /translation="MNLSEVQAAYFILNCLDPDNYTVKLYVKNGCKIFSADFLKLSET
                     GWSVIQESLDEDFDFSNPKISIASGAEKGFLWAKAAKTWNDSFSYPRQSVKLLDHPQL
                     LLEKIKAFIGNLQIEDYREFSTLAYNTEYIDGKKFNFCLLPESNYELFTIEVSEN"
     gene            15652..15867
                     /locus_tag="IV281_001747"
     CDS             15652..15867
                     /locus_tag="IV281_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780738.1"
                     /translation="MKTRETRAKRNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYG
                     NVVSVLSVHHQASVTVAEVEDAAVNGE"
     gene            16059..16271
                     /locus_tag="IV281_001748"
     CDS             16059..16271
                     /locus_tag="IV281_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780739.1"
                     /translation="MNENQLRELFKTNEANKTMEATFYETQKSLALIAKQSKYFYDQL
                     ILQGFNEGQAMEFMMRTFSASNQQKE"
     gene            16278..16505
                     /locus_tag="IV281_001749"
     CDS             16278..16505
                     /locus_tag="IV281_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780740.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIIEMTREAT
                     PNEIDKYNLRYCGHGYWKDEYIQQNIERYIK"
     gene            16502..16786
                     /locus_tag="IV281_001750"
     CDS             16502..16786
                     /locus_tag="IV281_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780741.1"
                     /translation="MNDNYDYIKLIEKIRAEKDMDELANLFMNIISLVGLKMDEVAAL
                     NYFIAEQTIRAEHNAKFLKDRLDLDVKGLGVEGIFKVQEALVNVYVEKMQ"
     gene            16827..17399
                     /locus_tag="IV281_001751"
     CDS             16827..17399
                     /locus_tag="IV281_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780742.1"
                     /translation="MKLKDYIREGYNIVTTPNLAYKIQEDYPNVLVFTDRALDAIPIG
                     KLLVDRYYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWHTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            17400..17711
                     /locus_tag="IV281_001752"
     CDS             17400..17711
                     /locus_tag="IV281_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8780743.1"
                     /translation="MIDVSTKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            17702..17962
                     /locus_tag="IV281_001753"
     CDS             17702..17962
                     /locus_tag="IV281_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780744.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEETTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            18130..18684
                     /locus_tag="IV281_001754"
     CDS             18130..18684
                     /locus_tag="IV281_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016630802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8780745.1"
                     /translation="MIGLNSKLVGTRMKQKRIEKGLTQADVATKSGISSRYYGSIENG
                     KNSPSIETLNSISIVIGCSLHFLLNDRMDDMEKRVELEKKRLQKIFDKIPHDKLNLVE
                     GLIIQAARLRVLLDDNWKDIIENGEYEKFKQSENQIAYDRKRPIVENYDNRDKTYQAI
                     VKQLTDLLPQGTRADKKSKLLGRK"
     gene            18687..20378
                     /locus_tag="IV281_001755"
     CDS             18687..20378
                     /locus_tag="IV281_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8780746.1"
                     /translation="MLFNQHVDQYIRKWKSGELILNEKRIQLLKLIEKEILPRDDIYY
                     FDEEQINNYIEFSQAWYFELDEWEKFISAFIFLFYKEDDEVVFDEFVINMGRGGGKNG
                     FISTLANYFVSSLHGIDYYDVSIVANSEKQAKRSFQECYRVINKKGNEDLKEEFEAYK
                     SSLTGLETQSVFEYKTSNASSQDGGREGAVIYDEYHEMENTDIVDVFSGGLGKVDHGR
                     QFFIGTKGFVREGYFDIKYRECEDILNGLSEFKGVFPYICELDAIEEMDDPKNWPKAN
                     PALQPPLNKRGKRLFNEVMKKYKKLAKEPSGRSAFVTKRMNFLEDNMENSVASREEIM
                     ATNRPFFELDTVPIGSLDFGSVRDFATCGLLFKNRKKSEYAFKSFTFAMKNFCDVHYG
                     YSLKEQLVGSEKRAPIKTWEKMGLMKVIDEPSLNPKYIVDWFIQAREEYGVRVIVVDN
                     YKADILGPMLEKEGFEVVRLKRPSSLHPLLAPRIEDGFANHKFIFGDNPLMRWFTNNV
                     YVKETQAGKQFLKKEEVKRKTDGFQAFVHALYKATELDEQIDYDDAFDMLDEIEF"
     gene            20410..>20749
                     /locus_tag="IV281_001756"
     CDS             20410..>20749
                     /locus_tag="IV281_001756"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780747.1"
                     /translation="MRIKGKNISWVFYMLGILAFTFVAIKLSEIIIPNDSVVSKNLVL
                     ERAKLIISILGPALTLIVFVRTIQTQLESSKNLIKDNVSKDFYNLLNLFKSIQDKMNS
                     ELFSTIEKRIK"
BASE COUNT         7283 a         3179 c         4156 g         6131 t
ORIGIN      
        1 attatctgtt tttttatatt aacgatttct atttggtaca tttttgatta ataaaaaggg
       61 gcaaaaaagg ggcaacaagt tacagttttt ctatatcttc tttcaatttt tcttttactc
      121 cttttgtcac atgcaaataa atcttttcag tgatatcact attttcgtgc ccaacgcgat
      181 cctgaatagc atacagagga gttcctagtt ccgctaattt tgaaatatgg gtatgtctaa
      241 aaatatgtga actaagtttt ttatcaatct tcatgtcagc tttatgattt ctcaaatagg
      301 tgttaattgc tgttagttgg aaaggagttc ctttcgttgt ttgaaataga aattgaccct
      361 tcggatttaa ttctagaagc tcattgtaaa tagctatcgc tttttttggt aaatcaattt
      421 ctcgcattcc agcagcagtt tttgtagaat cagatttttt catatcagct attgaacgtt
      481 ctctatacat catcgtccca tttataacta ctgatgcatt attattggta atgtgtacat
      541 cgtctttact taatgcgatt gcttctccag gtctcattcc agtcaaatat agccactgga
      601 aaaggagaga atatctttta ttgtgtgaag ttgtgaaatc tactagtctt ttatattcat
      661 catcttctaa aaacttatct ttaattttta tcgtttttga ctctcgttta taatcaataa
      721 ctacttcgtt gataggattt ttttctacat agcctttttt catagcgtat gaaaaaagaa
      781 gattcaattt ggatttaatt acactgacgt acttatttga caagtcatct ttatatatca
      841 tatcttcaaa aatattattt aaatctactg tattaatacc agaaacgatg taagtctcag
      901 gcatcttctt ctttatagtg tttaagatat tattcgtagg ataatatgta gactccttaa
      961 cttgcctttt gtaaataact aaccattctt cgacaagctc atgaaatgtt agattaggct
     1021 tctggagtgt tttttcattt agttttatat caattttttt atttaactcc agtaatgcca
     1081 ctttctgagt ttctcgagac ttatttttat aagtaatgct cacttttttt ctttttctag
     1141 tttttggatc aatatatcgt tcattatatt tatatacttt ttttccgttt ttatcagttt
     1201 tggtttcaat ccacattgtt ttcatctcct atttgttgct ataataggaa tggataagta
     1261 agccgattat agcaggttta tttttcatca cgtccacaaa ctttggcgag ggagggggcg
     1321 tgtttttgta ttttaacgat ttatattgaa tgttgattca gcactttcag caactgtact
     1381 ataatcagac ggattatata acactccggt ttttatcgtt acttttgtaa tatcgttaac
     1441 tttagatgga tctaagtaag ctaaactata aaattcttta ctttcagttt ttttgaaaaa
     1501 cgagtttgtt gtcattgtat gcataagatc gtctgaactg aaatcaattt gttggccgtt
     1561 atcaagtatg gcgtgtgcaa gcccattata accataatct acttcagaat tgtttttaac
     1621 tttgaatgca atttgtaaac cataaaaatt tccttcacta tctttgtgat tatttgcatc
     1681 gtagtaacca gcagcgctag ggtcagcata attggaatac ttgagtagtt tgatattaga
     1741 tatataaatg ccgatatcac caagaggaat atctgattgt gggcttgccg ttccaattaa
     1801 ttcaattttg ccacttgtat ctcttttttc tttggtccat tgaccaactt tgaggagggg
     1861 gccatcaacc tcctgtgaat ttttagaaga ctgtgttgta ctttctgata acgtaactga
     1921 agagtctttt tcgtttgttt tgccagtatt atctacaaca ttagattggc tacatccggt
     1981 aagaaaaaaa gcaccaaata ctaaccctaa aatcactttt ttcattcttt attctccgtt
     2041 tctatgatat tatttttatg taggatctta gaaacgagat cttagtccgt gttgcagcac
     2101 gggctttttt ctttataact tttttagaga ttataggcaa aatagtagga cacaaaaata
     2161 tattattgta ttccgtattt agaaaatcct aattgaactt caccagaagt tttttgctgt
     2221 gttgtacgca atgcttcttc ttcagacatt ccattctgta ctttccatgc aacaggcgac
     2281 atcccgtatt tgttaacaaa atcagtaagt gataaagtgt cagcgtcttg ctgaccgctt
     2341 gtttgttggt cttctggatt ttgttgagat gctgcttgtt gttctttctg atcttgactg
     2401 ataatattgc cagcatcatc tgtagtcaat ccattttcat aaagggccac gccgaaagct
     2461 tcccactctt tgttggacca atttgcacga tcagctggag ttgactgtaa agtgcgttgt
     2521 ttcatctgtt catatgtttc ttcttgaggt gcggtttgga ttgtatcctg actggagctt
     2581 ataactgttg ggctaggttc cgccgtagct tggttagagc ttgtaactgt tgaactagaa
     2641 tctgtcttag atgtagattt gctagtagag gaactggttt cagttgtttc ttttgtttta
     2701 cttacttttg tttcttggtt agaagtggca tctgttgatt cagctttttt attatttgaa
     2761 caagctgaaa gtagcagagc agtacttaac aacaacataa cgctaacttt tttcatttta
     2821 taattcctct ttctcgttgt aatatgtgtg ctaacacggg cttttttata taagaaaacg
     2881 ataagcgctt tctggaagtc cgtaaagatt ctttaattca tcgatttttt tgggatattg
     2941 atcattatct tctttataaa gagaaacaat gagattagca gcaaagcaat tagcttcgct
     3001 ttcagatttg cttctagatg ttcttgttga tacatagtaa ctggataagc cgcgatgaaa
     3061 aatagcgtga cctaattcgt gagcgcaaat gtagaatctt tcctcagagt ctcgcagttc
     3121 atcatttaag aagattattg cacgacctct aatttcttga aactgtcctt tgggattttc
     3181 gataaaagga acgtattgaa ttttaatgcc catcttttca caaatataaa aaggattagc
     3241 agaccggtat ttccgcttca actcctcgac taaattaatc gtatccatct ccataagctc
     3301 acatcttttt gcctttttct ttgtcttctt tcacaatatc ccagaaggtt ccaataagga
     3361 tatcttttac gcgttggacc tgttcaggcg tcagtgtttc tccgccataa gacatattga
     3421 catttgaatc tagtaattta tcaagttcaa ctacttcatc ttttgttgcc cagtcaggaa
     3481 cttggtttct accgagtaaa taatcagtag ttacattaaa ataatctgct acaagttgta
     3541 gtcttttagt actaggagtt ctatttttcc attgataaat cgtgttttta ggtatattta
     3601 attcttcttc caattgagtt atacttatgc ctcttttatg agctaattcc tttattctat
     3661 ctagtaaatt cattttcgca cctcacaagc tacgaaaaca ctaataaaaa aattagttaa
     3721 aggtgttgac aactaataaa attgttagta tactgttttc gtaagctaaa atattagcta
     3781 aaaagacttg ataactaata aacacttcac ggtcggcaaa ctctgaaatg taaattacta
     3841 ggcatttctg tgtcttattt agctatatct atatactaat aaaaatatta gttattgtca
     3901 acgaatatta gctaattttt tagcttacag actatttttt agaaaggagc tatttttatg
     3961 tctgagaatt tagacttaaa aattcgagcg gagatgagaa aaagaagaat gactttcaaa
     4021 gaactagctg cgcttgttgg tatttcagga gcttatttat cagatattct aaacggcaat
     4081 cgtgatggaa agaaagcaca acagcatatc gaaacagtga aaaaaatatt ggacatccga
     4141 tagggggggt aggcgatgac aaaactaaaa aaacaagatt ttgtaaaaaa atacaattat
     4201 tctccatcta cttatcaacg tcgaatgtcg gaactaaaaa atacagcaat tttctcagcg
     4261 gcgtatgaac gggtcacagg acaagaagtt tggatcaata cagaattata cgataaattt
     4321 ttgtctttca aatcctataa caggttacgc acaagaaaga taacacctaa agaatttatc
     4381 gagaagcatt tagttgattt ataaaaaaca gaacattttg agaggtgaag gttaatgggc
     4441 aaattcaaca gagcactagt attcagcaca ccattcatca tctacgcttt aggcctctgg
     4501 ggaagcagac aagcgttgat aggaacgatt gtttacatgg tttggatttt tatggggctg
     4561 gatgaagctg agtacaaaac aaaaaagcca gtcgggaggg actgactaat gaaaaaaagt
     4621 ttaatgacta taaacgaaaa acaattgaaa gaaaatttta atgatctcat caaagaattt
     4681 gtaaaagaaa ctggagaatt tcctaatcaa attcatctag ttgcggaggg gcatagccag
     4741 tatcaagctg tgaaatttga gatgaagaaa caaatctatt gaatttagca gtacctctac
     4801 ttgtaagaac atttattgta atgttttttt cgactaaaga ttcatcgtat ctaaaaacca
     4861 acgtagtaat ttttgactca tacggtggaa tggtaatagg taatgacaac ggaggaaatt
     4921 ttgatagatc agcacctttt tttaaagatt taccagaata cgcacgtata ccatgtggaa
     4981 gttcgtcata cattgatttt accgttacag aataattatc accaatcatt gtaaaagcat
     5041 ttaaatgatc aggaattcca gaaatcgaaa actcaataat tgtaacaggt aaagaactgt
     5101 tatttgttaa caatacagaa tctataatcc tgaatttatt ttgatggtaa acatctggtt
     5161 tggaatcact ttctctatct atcaaccatt cttcgcattc agcgaagaaa tccaatttta
     5221 aattaaattt tttgtttttt ctatctgtat aggagatata aaaagcaaat gttgaaaata
     5281 ctagagacgt aataggcaaa ataaaatctt taatggtttt aacaatgtta ttccagtcaa
     5341 attgtttaat aaagtcaata agactcaatt tataccacca ccatttttta tctacattat
     5401 atcaaagagg agagaagaaa taatgcaaga attagtaatt ttgaaaaata aagaagctgt
     5461 gactacgagt ttgcaagttg cagaaagttt tgagaaaaag catcaacatg ttttaagaga
     5521 tattgatgca ctgaaaaaag atgtgtccaa ttttggacag atgttttctg aaggtaatga
     5581 accagattca tacggcagaa atcgacgagt tttcttcatt agtagagatg gttttttctt
     5641 gttggctatg ggttttacag gaaagaaagc tatttacttc aaacaaaaat acattgaagc
     5701 attcaacgaa atggaagatg ttattcgcaa gaatactgtt cctcaaacaa ttgaagatat
     5761 gatgatctac caactaaaag aaatgaaaga tgttaaaaaa gatgtttcca tgcttaaaga
     5821 tactatgcga attagtgggc aacaagagtt tgaaattaaa caaaaaggaa atataaaagt
     5881 tatggaagtt ctagggggta aagaaagccg agcttatgaa gaaatcagca aaaaagtatt
     5941 ctcaaaattt tggtctgaat ttaaacgtac cttttcaatc ccaagatatg gcgagttacc
     6001 tcgtaagaga ttcgatgatg ctgtttcatt tattgaaatg tggttaccag aaactgcgat
     6061 ccgcatggaa atcgatcaac tgaacagaca acaaagactt ttcggtgatg aaaatgaata
     6121 gagctgaagc gctaagaatc gggacggtaa ttgctaatcg ctggtggaga cacaataaac
     6181 caatcatcct aagccaacaa catattgaca agcaaaaagc atggcaacaa ataaaaaagt
     6241 gactccgccg gcaagcaaag agtcacaaag aaaacacatc ataaggagat tttagcatat
     6301 ggaaaaagaa ctttccactc tagatcaata tttgactgat cctagttggg gcaaatcgaa
     6361 tatcaaggaa acaaataatc gaaaaatcag acgaaatctt ttgacggatg aagaactagc
     6421 atgtgatcaa gacgatttgg gaaattttgt gagtatttgg gatcatgtct atcttatcca
     6481 tttatcgagg aagtccaaaa aacctgaata tatctatgtc atcgaagatg gcttgattga
     6541 tgcgctagaa gagtatgaca gagataactt gattgatatc tcttattacg gatcaggtaa
     6601 gaaatacatt gctgaaatgg aggcagaatt tgatgagtga aaacaaaggg acaacgactt
     6661 ttgaaaaact ttttagtcgc aagttaaata aaatactcaa gaaaaaagga aattttgatt
     6721 atttatcttg ggctcacgcg tgggagatta tgaaaaagaa tgatccacag gcaacggtaa
     6781 ctattaatga gtataaacac tacagggttg tttctggaac tcatcaagac tttcttgttg
     6841 aggaatataa accttttctt atggatgaaa cagggactta tgtatctgtc tcagtaacgg
     6901 ttaaaggaca cacggaaacc gagttatttc ctgttttaga ttatcgaaac caaccagttg
     6961 ttaaaccaaa tgcaatgcaa atcaataact cattgaagcg atgctttgtg aaagcattgg
     7021 ctctacacgg actgggatta tatgtatttc aaggggaaga tattccaaca ccacctagaa
     7081 tcgatacgaa gaaattaaac atgctagaga cgattctaga agctttcaat gagcagatgg
     7141 gtaaagatat gaccaaaacc ttaatcgaat atgttaatga gcagacagat aaattagggc
     7201 tcttagctga taacgttgaa actattgaac agttaagcta tgagcaatgt gccttgatgg
     7261 agcgagcaat agcagctaag agaaaagaat tagataagaa gtgatatgag tgtttaaacc
     7321 attaatcgat tcatattcag cggttctgaa aaagttcaaa ggaaaagaca taggtgcaac
     7381 catcaatgag gaagtgaaca ttgatcgact aaagacgatg tatgacggct acgatggtga
     7441 tcgagttatt gaaattcgtt ttattgatcc tagacgtttc acagtacagc aacgaaactt
     7501 catctatgca ctcataggcg atattttcat cgatacaggc atgccaacgg acttctggaa
     7561 ggaattcttt tacttccgtt tcgaaggtgt cacagggcgc gaaataagcc tcaaagatga
     7621 atcgaatacg actgtgagtg atgccaatat cttagcaaat atcatcttag attttatctt
     7681 tgaacatcat attcctttca aagaaggcta tgagatttta ccagcgaatc aagaatatta
     7741 cttctacaaa tgcatcacaa aaagagtctg ctgtatctgt ggcaaaacag gagctgatat
     7801 cgatcacttt gacaaagcgc taggaagacg aaagcgcaaa gaagttgatc attcagagta
     7861 cacatttgca gcactctgta gaattcatca cacggagaaa cacaaaatag gtgtgaccaa
     7921 tttcaaaaat aagtatcaaa tcaaagggat taagttaaac caggagacaa tcaagaaatt
     7981 aagaatcggg ggctaatgat gattaattta aacgcagtag cgtttgaaca aacatggcgc
     8041 actaagtata aaaaaatgag tccaagagac aaattgttct tagaaatcat gacatttgct
     8101 ttcattggta cacaagctga acaaagcgat attagtgttg aaaaaattaa gacgaataga
     8161 ttagttaatg gaattacaga gacttgttac cagtacacga ttatcgtcgt ggatgaggag
     8221 gaataatttt ggcagaaaga agaatgttcg caaagacaat tattgattct gatgcattcc
     8281 tagatatgcc tttatcgaca caggcattat atttccatct atcaatgcgc gcagatgatg
     8341 atggatttat taataatccc aaaaaaatac aacgtatggt tggttgtggt gatgatgatt
     8401 taaaactgct tatggcaaaa agattcatct tagttttcga gagcggtgtg atcgtcatta
     8461 agcattggaa aatacacaac tatattcgta atgatcgtta caaacccacg ttatatcagg
     8521 atgaaaaagc tttactagca gacaaagata acaaagctta tacatttgca gaagagcttt
     8581 ctaaacatga tgaaaaactt ggtataccag atgacaacca agcggtatac caaatggata
     8641 cacaggttag gttaggtaag gataggttag gtaaggatag taaagagata aaagatttaa
     8701 cgccttcgaa aaaatcgaag gctaagccca tccgtcataa atacggagag tataaaaatg
     8761 ttcttttgtc agatgagcag atggagaaac tcaaaacaga attccctaat gattaccaag
     8821 agcgaataga acggctatct gagtattgtg aatcatctgg taagacttat aaaaactatt
     8881 tggcaactat tcgaagctgg gcaagaaaag aaaaaagtga atctaagaac gcaagcagtg
     8941 gatacaagcg cacaggaaga cgagagaagc ttcctgaatg ggcaatcgac caagaagcct
     9001 atcttaagaa aaaagcgcta gaacgagcta atagacaatc aaaagcacca ttttaagagg
     9061 tggaaaattg aaaatcgatt atctagaact aattaatgaa atagcgaatt ataaaactgg
     9121 cgaggaaata gacatcctga gagacgtata tgatcaactc gaagaagctg gaatcgaagg
     9181 aattaataat gatcgttcga gttggagtaa actcagatac tatttcgcac tttatatcga
     9241 tgcaacacaa ttaagaaatt tagcttatac aaaattacta tttgttgatt gcattaaagg
     9301 attgcaaaaa catcttagtg aacttgagca ggtgtaatca gatggatcta aaaacattta
     9361 cagcacagat cgaactaatg catcaagaag ctttaagaca aagcgcctcg tacgaagaca
     9421 agtggctcaa cacgttccat ggtggacgtg agagcgcact tgatcaagta ctcaaattat
     9481 tgaaagggga acgtcgggat ggataagaaa gcggcaatga aacgaatcat cgaactgaca
     9541 cattctgaga attggcaaga agacaaagaa atagttacag aagtccaaaa gctcggtaaa
     9601 tcaatgtgga ctgaaaagcc caaacggaga acgccgagaa agattgcaat ttggcatggt
     9661 gatcgaattc tagtaacagg tactgctgaa cagttatctg aaattactgg tctgagcaaa
     9721 aacatcatct gggatagagc aaggagctta tggattgatt caaaaggacg acaatttagg
     9781 tatgtggagg agaaaaaatg aacgaactaa tcacaaaagt agagcagtgg gctaaagaca
     9841 agggactgga tcaagcagat ccaaaagcac agtttttgaa agtagctgag gaattcgggg
     9901 aaatcgcttc agcaatggca agaagtaatg atgagctatt taaagatagc gtaggagacg
     9961 taatcgtcac gttgattatc ctttccatgc aaaaagggac aaacgtacaa gagtgtttag
    10021 aaatggcata caacgaaatc aaagggcgca caggaaaaat ggtagatggt gtattcgtga
    10081 agtcgagtga tttggaggac agcaaatgat aggtttaaca atattttggt gtgcgattgg
    10141 tggtttacta gcatttctag gatactacct catctgtgaa agtagaaaag gaagaaaata
    10201 tacagtgaca ggtattgtcc tgttaattat cggaattcta gttgcagttg gagcagaact
    10261 gaatctttat gtaaatggta cacaagaaga tttggtgcga tttatgtttt ggatgaagaa
    10321 ttaggaggaa aaaaatgaac gaaaacaaat taatcaaatt aggtgtagca ggagcagtaa
    10381 tcgtaggtat tggagttatc ggaggattta agttcttcga aaaaatcgat aatggatatg
    10441 tgggtgtgcg ctattcaatg aacggcggta tcaaagatga agcactgacg caaggtgtga
    10501 aatttgtagg gattgacaaa gtgatccaat atccaattcg cttgcaaact atccaatcaa
    10561 aaaatatttc agtatctaca agcgacggca aaaagacaac gattgatatc aaatatgact
    10621 acaaagttga ttcaactaaa gcagcaaaaa tgtacaagga atttgggaat atcacttcgg
    10681 aagatatcga aagtggatgg ttaaaatcta agcttcaaaa ggtcgctcgt gaagtttatg
    10741 cgaaatatag tctgcttgat gtcctttcag gagattcctc taaagttgaa gctgaggtat
    10801 taacgaactt tgctaaatca gttgaatcta aagggtttga agtcgaagac gtaacacttg
    10861 gtgttccaga tgtcgataaa gaaacacaaa aatcaatcga tgcgatcatt cgagctggtc
    10921 aagaaaatga aaaagcgaag ctagatgcag aaactgcaaa aactcaagct gatagtgaag
    10981 cttacaagaa aacaaaagct gcagaagcag aggcagaatc taatcgcaaa gtcgccgaat
    11041 cagtaacaga caatttgatt cgttatgaag aagctcaagc tcgcaaaaag catggatggg
    11101 taacagtaaa tggagcagat actgtagtta cggatgaagc aggcaaataa tatgggattc
    11161 tttatggcta aaattctctt gttcttaggt ttagtaggag cagcatatct cgtgtatgcg
    11221 ctcctttccc aaactgatga caaagaagat gacaacaacg atgaaatgaa attttaggag
    11281 gagaaataat ggatctcatt acacaataca gtgatatcat cctcaagaaa atcatggcaa
    11341 agattcagaa agataaaaaa gcaaaagaac gagcggaatt agttaagttg gaaatggctg
    11401 aaacaggagt aggagtgcga agtagcaggc attggaaagc agcagcaaac attgaatttt
    11461 attacaacga aattcaaaaa gggttcgatc agatgcgtga gctggatcgg caaacaaatt
    11521 ggagcaagaa acttcatcaa gatcgtttca aatttgtaga gaagtataaa gaaatattag
    11581 aagagtattt gaggaggaca gcgaatgata aaaaagctca ttcaattcag catggattta
    11641 tatgatatcg aatcaggagc aacagtatct gtggagtctg atcatttaat cataagtttt
    11701 gctgacgaac gtcaaattat catatgggta gttgacgata tgctatatcc agaaattgta
    11761 catgattttg aagaatcaaa agcggttgag tttgaaatag tgaaaaaagt aatggaattg
    11821 attgaaaaat acgaggagga cagcaaatga taccgaagtt tagagcgtgg gataagcgag
    11881 aaaacacaat gagagatgta gctgtcttac attttactaa aaacggcaaa acaaacttta
    11941 ttgaatattg gataaatcct accgaattga aatcatatca cgtgcgaaac atcgacctca
    12001 tgcaatccac aggaatgaaa gataagaatg gtgtggagat atttgaaggg gatgtagtaa
    12061 ttacatcaaa aggagcgatt ggatatgttg cgtatcttca acaagaagcc ggctttgtag
    12121 ttgtactaaa gaaaagtgat tatagattag gccataggaa tactggcgaa agttatgatg
    12181 tagcaactgc tcatgaagtc atcggaaata tatacgagaa tagcgagtta ttggaggtag
    12241 agtgattatc ttagctactg actacatcga ttctttaaaa gatgaaaatg ggaatgtccc
    12301 agcattgcta aattgtggaa ttaaaagcaa gaatagaatc attttgttac ggatatcggc
    12361 tgatttagca aaatatcttt atctgagcaa gacagcagta tattacactg cggctacacg
    12421 ttatcagtac aagggaaaaa caatcagtca ggattattat gatcgtttaa tcagtcctga
    12481 catgcatgga aaatcaaaat cagcgatcaa gttatttggc gcaatagaga tatatgccga
    12541 tgattttcca aatttatggt tgaaggagga agcggaatga aatacgaaat accactaagt
    12601 gaagcgggca ttcaagcaat tatcaatggt cgggaggtta ataaaaaact tcctgatggt
    12661 actgaattag tcatcagaca aagttatttg aaagatatgg cagctccagt attaattgat
    12721 cgttttaacg tgactgattc tgtggtagag aaccacttga aagaatttcg atcaagtata
    12781 aacgacactt tcagattagg gagttgattg acaatgaaca ccagacaccg cagagtagca
    12841 aaactaagaa aacaggaact gaatgtacta aagacaaagt ttgaaaaaga atatggaatt
    12901 tcagcagaag aaacatataa agtggcaagt cagtttgttg ctgatgcgag tgagactatt
    12961 cgtaagtttg ggatttcgat attaaatgat gatcgtaaat gggaggcaga aagatgaaac
    13021 taaaagacgg attttacact gatgcttatg gaattggtgg tttgatgatg gatctaccaa
    13081 ctaagaatcc tataaagcaa gaggaatcag aaattaaagt cggtgacatg gttcgctgcg
    13141 aagcagaagg gttcatctat ccatttcgtg gatatgtaga gcatctctat aatcactcag
    13201 cgatcattcg cattgaaaac acgatggaat gtgacaagcg gttagcgaaa agcaaagaga
    13261 atttagcagt ggtgagattg gtggatatgg aagttataaa atcttaattt tgctttttta
    13321 caactatcct acaacaatga gcgttattcc acaactggaa ggagaagagg caattgtgaa
    13381 cattttagag aatatagata ttaagcaaac aaggaaaaat gctagacgat tactaaaaag
    13441 atacagaaat ttagaacgtt tggtcggacc agtgaaaata gacttttccg tgatgactgt
    13501 taccaaaaat ttgaaattca caattgacag tcaaaacgaa gaaatcattg aagcgataag
    13561 tactcgagat tcggttatcg aagcattaac acggctaagt agaatccatt tccaggtact
    13621 ttattatagt tattgttttc ctaataagat gtcaatgtat caaataggag aaaaattagg
    13681 atattccgat agaactatcg agagaatgaa ggcagtagct ttggttgaat ttgctgaggc
    13741 gtataaatca ggagaactca tttcacgtac aaaataaaaa aagtcggatt cctccgacta
    13801 tgaataatat ttccgacata agtattatat cataattggg ggaatcagag gatggtactt
    13861 ttcgatgtaa agaaatatga gacaccaagc gcgaaagatg ttgatatgga acgcacaaaa
    13921 cataatgtcg ctgtgtttct ttcagcatat ctatcagcta gatgtagagt agggcaacct
    13981 cgagaaccaa aagtgacagc atcttattcc ttggttccac cttctacagc tgatcatata
    14041 tttgaagcag aaagaatgct gatcgataaa gaagaagcac aggaagaatt tgagtatctg
    14101 cataaattgt ttgttcgagg ctattcagcg atacagcatc cgcataagcc tgatgtgact
    14161 gaaaggcgca agaagatatt ctatgatcgt tatatcaatg gtctgcccat ttatgtaact
    14221 gctcaaagga ataatactag cgaagaatcg gttaaagtag aatcaaacag aattatcatc
    14281 caatttgctt catcgttaga actggttgct ttcaagtagc cagtttttac actttttata
    14341 cccttttatt accaatttgg tttccatttt ataccttttt tataccaatc acttacctat
    14401 tcaacgttgt attatgatag tgtcgaaaga ttagtgatag gtcttagaca aaataaaatg
    14461 taagggagga aatctccctc atcgttttaa attaagcctc gatagacagc agcggaaata
    14521 ttaagaataa ggatgtgaac tcaactcctt ctaaattgtt cttattatct atcatccgtt
    14581 gctgtctatt aatttatgta ttggagggta tataaaatga ataacgcttc cgaaaaaatt
    14641 gatcaactaa ctattttagt aaaccaaaga atcgaagata ttatcaagga attcgaaggt
    14701 gaaaagatta agagcataaa gcttacttta atggatcata agttcgaact aaattcgaca
    14761 aaaaggaaga tcgttgagct tatagaaaaa gaaattgctg tccagatcaa tgagcaacaa
    14821 ttaactaatt aatttatgca tcggaggaaa aacgaatgga taaagaaagt gcacgagaac
    14881 tttatttgag taaattcaaa cgtaacaaca aaccagaaga agtttttgga tcaaacgtta
    14941 aagaggttgg agaaaaacta actcaattat taaaagaaga agacttaaca tatgacgaag
    15001 catacgcaag tcttcagtac tgttacaatc tattgaagta tgagtctaat tttctgaaac
    15061 ttcaatagta aataattcgt aattggattc tggcaacaaa caaaaattga atttttttcc
    15121 atcaatatat tccgtattgt aagccaatgt agaaaattct ctatagtctt ctatctgcag
    15181 attgcctata aaagctttaa ttttttctaa taatagttgg ggatgatcca aaagttttac
    15241 tgattgtcta ggatatgaaa aactatcatt ccatgtctta gcagcttttg cccaaaggaa
    15301 tcctttttcc gcaccacttg ctatggatat ctttgggttt gaaaagtcaa aatcttcatc
    15361 caaactttct tgaatcacag accaacctgt ttcagagagt tttaaaaagt ctgcactgaa
    15421 aattttgcaa ccattcttaa catacagttt aacagtataa ttatcagggt ctaaacaatt
    15481 taaaataaaa tacgcagctt gaacctcgct taagttcata tttttcacca cctataattt
    15541 atttcagcgg accactcgct gataactaaa attatacgct tagtatttat tttcacaata
    15601 ttaatttgtc actgtggcgg aaagggtaga cgctatatct ggtaggtatg cttgaagacc
    15661 agagagacaa gagctaagcg caaccatgca aggttcgatt ccttgccagc gacattaaat
    15721 gcctatgacg gttacgacta ccgaaaaaag atcgttaaga agctatacgg tgctacgtac
    15781 ggcaatgtag taagtgtgct atctgtacac caccaagctt cggtcactgt ggcggaagta
    15841 gaagacgcag cggtaaatgg cgagtagcct cgtgagagcc tggtaagttc tcgtgagtgg
    15901 tgcaatccca ctccagcgac tttaagcaac cgaggcatcg gcggtttaaa aatatagggg
    15961 tgcgcaattt cgtacgcgtt ttgtgcatcg tgtaggttgc tattacatat tagatcactc
    16021 atcgagtgat ctttttattt tgaaagggga gtaaacaaat gaacgaaaac caattaagag
    16081 agttgtttaa aacgaatgaa gcaaacaaaa caatggaggc gacattctac gaaactcaaa
    16141 aaagcttagc gttaatcgca aaacaatcaa agtatttcta cgatcagctt attctgcaag
    16201 gatttaatga aggacaggct atggaattta tgatgcgaac cttttctgcc agtaaccaac
    16261 agaaagagtg atacataatg agaaactact ggtatatatc gctaactaat gaatatcctc
    16321 gaaccattga tgattgttca gtgcgtgttg tgcgttctgt acaaatcaaa aagaagtact
    16381 ccatcattga aatgaccaga gaagctacac caaatgagat cgataagtac aatcttcgtt
    16441 actgtggcca tggatattgg aaagacgaat atattcaaca aaacattgag aggtacatca
    16501 aatgaatgat aactatgatt acatcaagtt gattgaaaag ataagagcag aaaaagatat
    16561 ggacgaacta gctaacttat tcatgaacat cattagtttg gtggggttaa agatggatga
    16621 agtagcggca ctaaattatt tcatcgctga gcaaacaatt agagcagagc acaatgctaa
    16681 gttcttgaaa gataggttag atctagatgt aaaaggatta ggcgttgaag ggatattcaa
    16741 agtgcaagaa gccttagtga atgtttatgt cgaaaagatg caatagcatt aacaagtaat
    16801 tattggatgt tttcaggagg gaaactatga aattaaaaga ttatatcagg gaagggtata
    16861 acattgtaac tacaccaaat ctagcttaca aaattcaaga agattatccc aatgttttag
    16921 tgtttactga tagagcttta gatgccattc ctatagggaa gttattagta gatcgttatt
    16981 atagcaacaa tccagctgtt cttagtgcta agccacttca aaacgcttat gctattgaac
    17041 gctttacttg cgagtttact ggatatgaga cggtagtacc aatgacaggc gagacaggaa
    17101 gaaaagtaat tcaacagatc aaaaagagga ttgctgaatt gacgttagat gatggaagta
    17161 atacgaacct tctagaaatc aaactaagag atactgactc agtaccagaa gtttattata
    17221 aaggtcaacg aatggatgag atgccgatag gtcttgtcga tattacttat cattggcata
    17281 cggaagaata tgaatgccaa gtaccaaaag gagctaacga catcactatt gaatattatg
    17341 attcacataa taacaagtac ttagatagaa aaatcattgg gcacaagaga gatatgtaaa
    17401 tgattgatgt ttcaactaaa caagcaagag ctaggttcta tggatcaact gagtggagga
    17461 aacttagaag tcaatgctta gagcgagatc attatgagtg ccaatggtgt aaagcagagg
    17521 gtagactaac aactcaacat gattcaattc ttgaagtgga tcatataaag gagctggagt
    17581 atcatccgga tttagctcta gatataaaca acctaagaac cttatgcaaa gattgccata
    17641 acaaaaggca caaccgtatg aaatatcgtg gccaacaaaa gaaaagaaag tgggatgatg
    17701 aatggtggta agaaaaacag caaacagaga tgatgtcaaa cgagcagaag caatcgctat
    17761 ggttgataag gctattgata agttggttct atttaagagt cagctcattg aagaaacaac
    17821 aactgtagag gatatatcta tcaacaatag actaataggt aacgctaatg aagtaacagg
    17881 gacatttaat attaatgtag acattgatta taccttgagt ccatttgcac atattgtaga
    17941 aagcaacgag caagacggct gataaatcag tataaagcga agatatatag agtgaaatac
    18001 ccccggtcaa aagattttga gaagaaataa aatttttggg aaccggtgga tggggttaat
    18061 tctgcaaatg caacgtattt attttacccc tcccccctcc gccgcccctt tagtaaatat
    18121 agaaggaagg tgattggttt gaattctaag ttggttggca cgagaatgaa acaaaaaaga
    18181 attgaaaaag gattgacaca ggctgatgta gcaacgaaaa gtggtatttc tagtaggtat
    18241 tatggaagta ttgaaaatgg gaaaaattcc cctagtatcg aaacgttaaa ctcaatttcc
    18301 atcgtcattg gatgcagttt acatttctta ctcaatgatc gcatggacga catggaaaaa
    18361 cgcgtagagc tagaaaagaa acgtctgcag aagatattcg ataaaattcc tcatgacaaa
    18421 cttaatttag ttgaagggct gatcattcag gcagcaaggt tacgtgtctt gttggatgat
    18481 aactggaagg acatcattga aaacggggag tacgagaaat ttaaacaaag cgagaaccaa
    18541 attgcttatg atagaaagcg tcctatagtt gaaaattatg ataatcgaga taaaacttat
    18601 caagccatcg tcaaacaact caccgattta ttaccgcaag gcacaagagc ggacaagaaa
    18661 agtaagttac tcggtaggaa ataaaaatgc tttttaacca gcacgttgat caatatattc
    18721 gtaaatggaa atctggtgag ttaatcctta acgaaaaaag gatacaactt ttaaaactaa
    18781 tagaaaaaga aatattacct cgcgacgaca tttattactt cgacgaggag caaataaata
    18841 attatatcga gtttagtcaa gcatggtatt ttgagttaga tgaatgggag aaatttattt
    18901 ctgcattcat ttttttgttt tataaagaag atgacgaagt tgtttttgat gaatttgtta
    18961 ttaatatggg gcgtggcggg ggtaaaaatg gctttatttc aacattggca aattattttg
    19021 tcagttcgct tcacgggatt gattactatg atgtttcaat tgtagctaat agtgagaaac
    19081 aagcaaaaag aagttttcaa gaatgctatc gagtaatcaa taaaaaagga aatgaagatt
    19141 taaaagaaga gtttgaagcg tataaaagca gtctaaccgg tttagagaca cagtctgttt
    19201 ttgagtacaa aactagtaat gcaagctctc aagatggcgg tcgagaaggt gcagtaatat
    19261 acgacgaata ccacgaaatg gaaaacacag atattgtaga tgtgttttct ggtggactgg
    19321 ggaaagttga tcacggacgg caatttttca ttggtactaa ggggtttgtt cgtgaaggtt
    19381 actttgatat taaatataga gagtgtgaag atattttaaa tggtttgtca gagttcaagg
    19441 gtgtatttcc gtacatatgc gaactggatg ccattgaaga aatggatgac cccaaaaatt
    19501 ggccgaaagc aaacccggca ctgcaaccac cgttgaataa acgtgggaaa cgtctgttta
    19561 acgaagtcat gaaaaagtac aaaaagttag ctaaagaacc ttctggacgc tcggcatttg
    19621 ttactaaacg aatgaatttt cttgaagaca atatggaaaa ctctgttgct tcaagagaag
    19681 aaataatggc aacaaatcgt ccattttttg aactagatac ggtaccgatt ggatctctcg
    19741 atttcggtag tgttcgagat tttgctactt gcggtttgct atttaaaaat cgaaagaaat
    19801 cggaatacgc gtttaaatca tttacgtttg caatgaaaaa tttttgcgac gttcattatg
    19861 gatactcgtt gaaagagcaa ttggttggca gtgaaaaacg ggcaccaata aaaacttggg
    19921 aaaaaatggg gctgatgaaa gttattgacg agccatcttt aaatcctaag tatattgttg
    19981 attggtttat tcaagctaga gaagaatatg gagtgagagt aattgtcgtg gataactata
    20041 aggcagatat tttagggcct atgcttgaaa aagaaggatt tgaagtagtt cgactaaaaa
    20101 gaccatctag tttacatcca ttattagctc ctcgtataga agatggattt gcaaatcaca
    20161 aatttatttt tggagataat ccgttaatgc gttggtttac taataatgtg tacgtaaagg
    20221 aaacgcaagc aggcaaacaa ttcttaaaaa aagaggaagt caagcgtaaa actgatgggt
    20281 tccaagcatt tgtacacgca ttgtacaaag ctacagaatt agatgaacag attgattacg
    20341 atgatgcttt tgatatgttg gatgagattg aattttaagg tatacttttc tttacaaagg
    20401 ggtgctttca tgaggataaa aggaaaaaac atatcttggg tattttacat gttggggatt
    20461 ttagctttta catttgtggc aataaaacta tcagaaatta ttattccaaa tgacagtgta
    20521 gtttcaaaga atttggtttt agaaagagct aaactaatta tttcaatttt aggaccagca
    20581 ttaacactaa ttgtttttgt tcgaacaatt caaactcaat tagaatcttc aaaaaactta
    20641 attaaagata acgtcagtaa agatttttat aatttgctaa atttattcaa aagtatacag
    20701 gataaaatga attctgaatt atttagtaca attgaaaaaa gaattaaag
//
LOCUS       DACDIH010000044        20326 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDIH010000044 DACDIH010000000
VERSION     DACDIH010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.044"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..556
                     /locus_tag="IV281_001757"
     CDS             <1..556
                     /locus_tag="IV281_001757"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780748.1"
                     /translation="TKEVQEDLKEYSASLNKVLSESVKKINEIFEDNYKDIGSYLKVF
                     HRILKILNNRYESKDISHEEYKMYVGILRSQIEDYEFYIILINSLFTLRGTGMGIELI
                     ETSFFGDELDIDLKQHFEHPEFLTEIIKDNFVEKKSYRESVKTRKKNRKLFENRKIND
                     NLLSLLRIRYTLQDGNKIIISNGV"
     gene            605..1777
                     /locus_tag="IV281_001758"
     CDS             605..1777
                     /locus_tag="IV281_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8780749.1"
                     /translation="MSLFDIFKQSIKNEDPSDWIPDFVFGDEESARAYLKIMAKSTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWAMSDVIYMEYNNEELERFTR
                     GLFEDYAELFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDKIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIADVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            1764..2468
                     /locus_tag="IV281_001759"
     CDS             1764..2468
                     /locus_tag="IV281_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP8780750.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            2473..3633
                     /locus_tag="IV281_001760"
     CDS             2473..3633
                     /locus_tag="IV281_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8780751.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTVEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVEYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            3709..3987
                     /locus_tag="IV281_001761"
     CDS             3709..3987
                     /locus_tag="IV281_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP8780752.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            3968..4339
                     /locus_tag="IV281_001762"
     CDS             3968..4339
                     /locus_tag="IV281_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP8780753.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            4355..4759
                     /locus_tag="IV281_001763"
     CDS             4355..4759
                     /locus_tag="IV281_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780754.1"
                     /translation="MSNVKFEGVQETIQALEKKFGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIDEVVRKNATYRNYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSEPLYFDAVGEEVKKRL"
     gene            4756..5166
                     /locus_tag="IV281_001764"
     CDS             4756..5166
                     /locus_tag="IV281_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780755.1"
                     /translation="MKDMLNIIYEALCLNEYIRSMTYNENTEQYRIKFYEQPETADKT
                     GPMITLRPVDTPNEAYHGSNKELSVEHMVQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFSETKRFVDARRYEGNTKIYDTEY"
     gene            5227..5814
                     /locus_tag="IV281_001765"
     CDS             5227..5814
                     /locus_tag="IV281_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8780756.1"
                     /translation="MTLVGFKKMTIGIFDSTGKIPAANLYVIEGEQDKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITALKEMVFPAAGE"
     gene            5887..6354
                     /locus_tag="IV281_001766"
     CDS             5887..6354
                     /locus_tag="IV281_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HAP8780757.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            6400..6708
                     /locus_tag="IV281_001767"
     CDS             6400..6708
                     /locus_tag="IV281_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780758.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            6903..13667
                     /locus_tag="IV281_001768"
     CDS             6903..13667
                     /locus_tag="IV281_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8780759.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGITGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRDLATKLPASHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKMLKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKALDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDNTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDIVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPNDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPNAKGTNFHPG
                     GAAMVNDQKGPTYEELITLPSGEAFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYADG
                     IGNITTVSSTLNLDALILAINELTTVLRTQLMAETTNAKDTKLSDETKVADPIIPGSL
                     SEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGVAW
                     NKNLLNGWNSLTGTFISTINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYGS
                     FIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMVG
                     GMGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHEE
                     YVQMPDGRGFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDLL
                     DDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDGA
                     VNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRYT
                     AAANYAFEKFPSKIAYVITNGRVRDRMGLSGTGSSGQWTNWPDGDHFDHIHLNGSMGS
                     GDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGND
                     GLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIGK
                     GHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGDDKKRSNTT
                     NTADNSAELVTLIKQQQKQHNELMMILRAILGKDLSLKSSDIGQAANHYMGSDLNKLR
                     YTNGGV"
     gene            13671..14492
                     /locus_tag="IV281_001769"
     CDS             13671..14492
                     /locus_tag="IV281_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HAP8780760.1"
                     /translation="MFYKLQFNQNGKLFDPQRKEKIVCKEIKRQAPVYEVNYEDFEGT
                     NGSREANASFRPFELVLTFDIFYKNKHDKELLLTEFYELIFVGYQYYISYDLSPGKRF
                     KVNPTNFEITEEENDYSTIEITFSIPSGSAESSATTLTDFNLEEEWQFSQGLVAEDYK
                     YTHQTSHFIIYNAGSFEIDPREHYLRIALEGESEGNVTIFNKTTGDRFIYYPSLSTNL
                     GQTLVLDGVIPKLNGVSCGINTNHGLINLVEGVNEIEIQNITRVKSSWDFRFLYK"
     gene            14501..15544
                     /locus_tag="IV281_001770"
     CDS             14501..15544
                     /locus_tag="IV281_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780761.1"
                     /translation="MTELIIRNYEQTKEEILVGYDKGSFYENWQQNETWEIGFTVTSN
                     SLNQEVFDLVEYESSVFYNGQEFVIKEMTRKALGQLLTKQVVATHIYYTVQDGYQYNT
                     VTGAKSISQLLTHVFSAGSRGFTWEVIDPNKKFLTVEQENFGNANYLKLINEILSDYN
                     AVVIPNNKHLTFYPASEYGQQTEEQIRYKYNTDEVSFDIDTYSLKTQIKGYGKLKDGA
                     NTENPKDSDYIFTPITYTSPESQKWGIRIQDPVKDERYTVSGNMLERLKTDLQDYPNI
                     SGSVTLKWKISPNKGDHVPFIYEPLNINTYIQVVGIKTYPAIPNKPPEITLSNTKKTM
                     TSILAEMAKKGVI"
     gene            15544..16575
                     /locus_tag="IV281_001771"
     CDS             15544..16575
                     /locus_tag="IV281_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780762.1"
                     /translation="MGLLKLISNRISTEWKEKFNKNIDYLNDLEKKLSDQDKSMNSRI
                     DNLVLHSGGESPNEVVDARVNNKGETFPTLHGRLVEHETLTDEQISELNTNMDSQKEQ
                     VQQLNKSVQQIIGGYNEPINIYVSKDGSDILGDGSEEKPFLTIQTAVNNVPLITTSQI
                     TIWVGSGAYLEDIMIRNLNFTSFLIRPIDNFDTIDPLKSDLPVKIRSICFTACKGYCQ
                     VAGMQIVDTANGADYGIRNEQSGYMAINRCKFAENTKTLARYNAVYVGGTSKINMYGD
                     TTLINQKVAIYAVLMGEIFVSAFGSGNDVGILCENGTVRGTVSTSFATTPTKTTGYGL
                     IITKGTVLQ"
     gene            16575..18677
                     /locus_tag="IV281_001772"
     CDS             16575..18677
                     /locus_tag="IV281_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAP8780763.1"
                     /translation="MVYKTNESIIMIQAEATSPNRTNVVFWSHDRGTAKLRMKLVRKN
                     GIPQSLPEGTTVPIRLMLKSATAEGGYGKHDYLATIEDRVNGIVSIVLEDNILGYVGI
                     VEGSVYIDFPNDRSLDTAGRFTFDIKRSPIDDSTPELEDYYFNGFSQTIDKIEKILAD
                     GKQEIEQKITESETQIDGKLKETSNKITKANQDVATINTNIDKANDRIDQTNQQISDL
                     GKLKKMYSNSIDFGNYDYSGNPNLMANINADSFSQGSGALSVVDDGDEVVITLDPNHK
                     LEIFKAKSQPALVVGKTYTMSVEIMLEDDFTGDPSNISLRYIKMPSWLTVLYTPYTLT
                     NTKGVWQKLTVTAKMTTAIDNAESWFIMLQNKDANNSLSGKLRLRHAKLEEGSTATPF
                     QPNLLAEPYNMCREYPNENIADPKVKFPIESGDHQIYQGYTEEELMIGQTYTITLKGT
                     KPASQTFVAYNHWTARLGELKPVDGLTDVWSLTFTPTNVVAMPKLFRVYQYPRSTVGA
                     CQIDWLKIEKGNTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKGLNNAVNVYD
                     PQRVEGLKNFADGIQIAGDKVISENDCTVYTLTKDNSQSFIDGYATFIKHGKEVVVNG
                     TVKFKKAYAFGVPLDDEVPDEFIARIVHGMLTGQSGTNSVSKAMYVQKDLGKIMTNSE
                     FAANEWFTFHGNYWVGVK"
     gene            18678..19124
                     /locus_tag="IV281_001773"
     CDS             18678..19124
                     /locus_tag="IV281_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780764.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGLRADGEPL
                     TIADQMFDPKLNQWIVLANTLDHNDLNNLKAMYEALEHENGDLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPTLKGGE"
     gene            19126..19263
                     /locus_tag="IV281_001774"
     CDS             19126..19263
                     /locus_tag="IV281_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8780765.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            19298..19540
                     /locus_tag="IV281_001775"
     CDS             19298..19540
                     /locus_tag="IV281_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8780766.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKNNNKWAWGYMIGLGITIIGYFLTKL"
     gene            19556..19753
                     /locus_tag="IV281_001776"
     CDS             19556..19753
                     /locus_tag="IV281_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8780767.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASALVAMLGKAYGWNGTDMIVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            19764..>20326
                     /locus_tag="IV281_001777"
     CDS             19764..>20326
                     /locus_tag="IV281_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8780768.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQIA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLA"
BASE COUNT         7224 a         3251 c         4360 g         5491 t
ORIGIN      
        1 tactaaagaa gtacaggagg atttaaaaga atatagtgca tcattgaata aagttttaag
       61 cgaaagcgtt aaaaaaatta atgaaatatt tgaggataac tacaaagaca taggtagtta
      121 tctaaaagta ttccatagga ttcttaaaat actaaataac aggtatgaat caaaagatat
      181 ttcgcatgaa gaatacaaaa tgtatgttgg catattacgt agtcagattg aagattacga
      241 attttatata attcttataa attctttgtt tactcttcgc ggaacgggta tggggattga
      301 gttaattgaa acatcattct ttggtgatga actagatatt gatttaaaac agcatttcga
      361 acatccagag tttttaactg aaatcattaa ggataatttt gttgaaaaga aatcgtatcg
      421 tgaatcagta aaaacaagaa aaaaaaatag aaaactattt gaaaatagaa agataaatga
      481 taacttatta tctcttttga gaataagata cacgttacaa gatggaaata aaattattat
      541 aagtaatgga gtatagtcct agaaataggg ctttttattt taccttgaaa ggtggtgaaa
      601 atatgtgagt ttatttgata tctttaagca atcaataaag aacgaagatc catcggattg
      661 gattcctgac tttgtgtttg gtgatgaaga atctgctaga gcatatttga aaatcatggc
      721 taaaagtaca gttcttgatt ttgttgcaag aactatgtca acgttagaag ttaagtttaa
      781 aaataaagat gggacatcag attgggatta cattctaaat gtccgaccta attctgatat
      841 gtcagcagct acattttggc aaaaattttt ttatcggctc ttagatgaca acgaagtgtt
      901 agtgatattt acagatgata atcaattgct gattgctgat gattttagtc gaacagagta
      961 tgccgtgtat gatgatgttt ttacaaatgt gatggtgaaa aattttgtct ttgaacgttc
     1021 atgggccatg tctgacgtca tttatatgga atataataac gaagaattag aaaggtttac
     1081 acgaggttta tttgaagatt acgccgagtt gttcggacgg attctagaag ttgcaatgcg
     1141 caataaccaa attcgtgggt ctgtctccat tgattcaaca ggaacggcaa atgatgaaaa
     1201 aggaaaagat ggtaagacta gatctcaaag attacaagaa tatattgata agatttggaa
     1261 ctcttttaaa actaactcag ttgcaattgt tcccaaagtg aaaggctttg agtatgagga
     1321 atacaccaat aaacaaggcg tatcgaatca atcactagat gaactgaata agatgaagtc
     1381 gtctttaatt gccgatgttg ccaacgccat aggagtacct acggcgctta tttatggcga
     1441 aaaagctgaa ctagattcaa atattaaagc ttttcgtaaa ttgtgtattg ctccactagt
     1501 taaaaaactt caggacgaat tggtggcaaa aataattagt cgacaagagt acaaaaacgg
     1561 ggaacgtata gaagttaaaa aagttctcca aaaagatcta ttagagtatg ctactcaaat
     1621 tgacaaaata acatctggtg gtgcgtttta tattgacgaa gttcgtgatg aagttgatta
     1681 tgatgagttg ccaaataatg aaggaaaaat tcgtattcgt acgaaaaact atgaagaaac
     1741 tgtgaaggga ggtgagaacg acaatgccga aagctaaaaa agtaccattt cagtttacta
     1801 acgaaattca gaatggtaag cacattctca ccttgagtgg aaatgtacag aaaaaatatt
     1861 ggcgtgatga tgatgtcata aacgctaaag atattcgtga ctctctagat actgtaaccg
     1921 atgatattgt aatcaagttg aacagcccag gcggtgatgt gtttgaaggg attgagattt
     1981 acaattattt aaaagatcac ccatcaaata ttactgtaga agttacaggg cttgccgcaa
     2041 gcgcagcaac gtttattgtc gctggtgcca atgatgtgat tatgaatgtt gggacttctt
     2101 tgatgattca tgaagcttct acatttgctt gggggaataa acaggacatc caaaaaacgt
     2161 taaacgcatt ggaaacgatc gatgattcta tcttagctat ttattcagat aagactggcc
     2221 aatctgttga taagttgcgt gaatggatga atgaagaaaa atggtttaca gcagatgaag
     2281 ctgtcaaata tggatttgcc aattctgtga agcgtgaaga accacaaaac gaacctcaaa
     2341 atattgcagc gatgattcaa gatgctgttg ccgttgcaat agcgaattta aaggaacctg
     2401 ttactaataa aatcgaacaa gaatcaaaac aaaaatcatt aatcgcacga ttgcgaaaag
     2461 gagaataaag atatgttaaa aattacagat aaaactgcag atgcaaaaaa ggcatttaat
     2521 gctatttctt cgaaagaaga tgcaacaccc gaacaaatta atgctgctct agaagaatat
     2581 gtaactgcca ttgcagaaga tgccggcaaa caggtgcgtg ctgaatatga agaattgaaa
     2641 aatgtgacgg acaaccgtgt attagaggct cgtggaattc ctacactaac agtagaagaa
     2701 acaaagtttt ataatgaagc cgtaaaatct ggaggtttcg attctgacgt agtttggcca
     2761 gaaacgattt tggaacgtgt ttttgaaaat cttcaaaacg atcatcctat tttaggcctc
     2821 attaacttta caccaactgt aggtcgtgtc aaagtaatta gagcacgtcg taaaggtgtt
     2881 gctgtttttg gaccattgca taaagacctt gaaggccaac tagatgctga atttggtgca
     2941 actgaatttg ttcaacttgc gttgaccgca tttttcttaa tttctaatga taccttagat
     3001 ttaggaccac gatggattga ccgttttatc aatctttctt taagtgaagc agtacgtgat
     3061 gtttgggcta aaaaaattat tacaggttct ggtaaagatg agccaattgg attgcttaaa
     3121 gatctagatg gtgcggtcac aagtggtgta tatccagata aagctagcgc agggacatta
     3181 acttttgcga aagagaagat tgttcttgaa cttgcagggg tgatgaaaaa attatctaaa
     3241 tacacataca aagtagatgg tgacgatgaa ggtgaagtgg aataccgttc tgttgctgga
     3301 cgagtgtatc taattgttaa tcctgttgat tactgggata tcgtagctag agttacattt
     3361 gcaaatttaa acaatgtgta tgggactacg atgccattta tcgctgttga tcatatcatc
     3421 gaatctgtag acgtaccaga aagtaaatgc attgcatacg ttgataaaga gtatgaagct
     3481 actcaatcaa gagctgaaaa agtctatgta tataaagaaa cttttgcaat gaaacgcgca
     3541 actttgtatg ctgtcgacat gcttggtaat ggctatccaa caaataatga tgcagcacaa
     3601 gtatatgact tggactttac agcgggggag taacgtcccc tgcaattggt gagataacac
     3661 cgacgactga tggggcagtc attaatcttt cgtaaatggg gttagagtat gaacactgaa
     3721 gaatttataa aagaatataa ggcccgattt cgtattttcc atacttccga agatgaggat
     3781 atcgggaaac agttagagag cgggtttgcc gatataaaat ctctaatcgg cagttttgat
     3841 ccttccactt atgagaaagg aaaagaatta gtttatgaac gtactcggta tctacggaac
     3901 gaatcactag agtactttta cgacaatttt caaacaatga ttatggatgc atcaattgat
     3961 ctggcaggtg ctggatatgc caattaaatc aaattataag aagcctaaaa ttgtagctgg
     4021 tgatctaaat acgccagtta ctttttttga gtttcaacct aataagggtc cagaacctgg
     4081 tgatgaagaa aaaaaggaac tgtatttttg tacagcacta gtttacaacc catcaatgaa
     4141 agatcgtgac attcttggca caaaagaagg cgttacgata aaaatacggg atccccatca
     4201 atcttatatt cctactaaca aacataaagt aaggatatta gattatcgat atggagataa
     4261 agtttgggaa ataatcgatg tctcaccgga tttggaagaa aattcaattg taaagatagt
     4321 attagaatta attacttaaa tttggtgata aacgatgagt aacgtaaagt ttgaaggcgt
     4381 tcaagaaaca atccaagctt tagaaaaaaa gtttggagaa aagaaaacta agacattaac
     4441 aaaaaaagca atcaatgttg gcgctgaaaa agtagaaaaa cagttgcaaa cggacatgac
     4501 cgtttttaaa gacaaaggtt atacgattga tgaggttgta cgtaagaacg ctacgtatag
     4561 aaattacaag gcagaagctg aaatcggttg gaatggtcct catcagcgtt atagaatcat
     4621 tcacttaaat gaatggggat atacacgaaa aggtaagcaa attaggccaa gaggttttgg
     4681 cgtaatcact aaatcgctta aaaattccga gccactatat tttgatgctg ttggggagga
     4741 ggttaaaaag agattatgaa agatatgctc aatatcattt atgaggctct ttgtttgaat
     4801 gagtatattc gttcaatgac atataacgag aataccgagc aatatcgtat taaattttat
     4861 gagcaacctg aaaccgccga taaaacaggg ccaatgatca cacttcgtcc tgtagataca
     4921 cctaacgaag catatcacgg aagtaacaaa gagctttctg tagagcatat ggtacagatt
     4981 gatgttgaat cgaaatatag agcaacatgc aagcagattc aatatgagat aaaaaaagag
     5041 atgaaaaaac taggctttgg ccaaatttct ggtcaagggt tagatgaata cttttctgaa
     5101 acaaaacgct ttgtagacgc gcgtcgttac gaaggaaata caaaaattta cgataccgaa
     5161 tattagaaat taagacacga aaataacgtg tcttttttta ttacccaaaa ttaggaggaa
     5221 aattttatga ctttagtagg attcaaaaaa atgacaatcg ggatctttga cagcacaggg
     5281 aagattccag cagcaaattt atatgtaatt gaaggtgaac aagacaaagg ggcgactgta
     5341 tctgctgaaa tcagtgggct atcaaaagaa ccatcaaaag tttacggatc gaacatcgct
     5401 tactatgtct ctcaaaaagg aacaggcgat gtttcagcta cttttggatt gttagattta
     5461 ccaacagaag taaatgacaa aattcttggt tacaaaaccg atacaaacaa aatcagcttc
     5521 ttgggagaag atactgagcc accatattgt gcaattttga tggaatcgga ggatttgaac
     5581 ggcgatacag caatgcttgc gatgttcaaa ggaaaattca gtcgtgaatc tattaatctt
     5641 aacactacga caaatgaagc tttcgaacca gaagcagaag aatatgtatt ttcagcgatt
     5701 gccaatgatg ttgaaggtga tgctaaaggc caaacagtcg caaaatatgt tggagacgaa
     5761 gaagcttcta ttactgcttt gaaagagatg gtatttcctg cagcggggga gtaactagct
     5821 ccgttgttgg agcaatcact cccactatta atggggtaac agttgaatta agttaggagg
     5881 ataactatgc cagatacatt taaaatctat aaaaaaaatg gtactgaatt tacaaaagtc
     5941 gcagagggtg aaagtccact ttctattact ggaattgcag caaatactca agttgctaaa
     6001 ggagactacc aagctacgcg tgtggttgga ggtcaagaat cagtaaaagt agatattcct
     6061 gcatttacca ctcttccaat ttcagttact ggtgtgacgt tggataaaac gacagctgaa
     6121 gtcgaacaag gcggaacctt aaaattaact cctactgtaa caccagcaaa tgcaacagac
     6181 aaaacaggaa gctggagcag ttcgaataca gctgtagcta ctgttactgg aggcaacgtt
     6241 acagtaaaaa cagacgctga agttggcgga actacagaag tttcatttac tactacggat
     6301 ggcggtaaag tagcaaaatg tactattaca gttattgaaa aagcagcggg ctagtcttcg
     6361 gactagcttt tttatttgga aaataatgga ggaatctaaa tggcaaaagt aagaattgaa
     6421 ttgaaagaag aatcaggaaa gaaagtctat gaaaacatag acacaacagg taaagattat
     6481 cgaaaagctt tagaaacaat caaaaagcta aatgaagatg gagtaatgat ttggaatcgt
     6541 ttagacattt atttggattt tgctgtaggt atcttcaggg atagcaaatt gacagctgaa
     6601 caaattcttg atggcttacc atctgagaag gtaatgagca cattggacga catcctagga
     6661 gaagtaatgg ggattgaaag taatccagac ccagacgcaa aaaagtaaca cccgaagaag
     6721 cagaagaaat gtacttaaac ctatgcagag agttagtgaa agcgggatgg tcactatctg
     6781 acatagaaga taattccttt gaaatgatga tgaaaattgc gtgcacttcg cctaagaaag
     6841 agaaacaaaa agaagttgat cttaaagact tcttgaaatc catgtagaga ggaggtaaac
     6901 atatggcaaa tggaaaacct ttaggcaata tgattataaa actagactta gacagttcag
     6961 ctttttcaaa aggtctagcc ggagctaaaa acgctgttaa ccatcaaatg aaagcaatga
     7021 aatcacagat gcaagtaatg aactcttcag ggaattctct cggtgcttta caagcaaaat
     7081 ataatggttt aggcggagtt ctacaagcaa atgaaaagca agtagaattg ctcactaagg
     7141 cttataagga tagttttgac tctaatggaa acgctactgc ttcgactgct aaatacgcta
     7201 atcaattgaa tcaagcaaca gctagatcag caagttacga atctcagatg aaaacaaccg
     7261 ttggacagat tgctcggatg aaagtggaaa cggaagggat tacggggaaa ctaaaagcta
     7321 attctgatgt attgattaat tctgggaaaa aaattgaagc ctttggtaaa ggagtttctg
     7381 ggataggatc tgccctaact gttggagtaa cagctccaat agtggcaggt gcaactgctg
     7441 ttactagtgc agctatttct tgggaaagtg cttttgcagg agtaaaaaag acaaatgatg
     7501 aagttgtaga ttcgaatggg aacgtagttt actcctacag tgatttagaa aaaggccttc
     7561 gtgatttagc gactaaattg ccagcaagcc accaagaaat agcgaacgtt gctgaagcag
     7621 caggtcaatt agggattaag acagaaaatg tagttagttt caccaagaca atgattgata
     7681 tgggcgaatc tactaatatg tcagcagaaa cagctgcaac ttctttagca cgatttgcta
     7741 atataaccca aatgtctcaa aaagactttg ataaattagg atcagtcatt gttgatctag
     7801 gtaacaattt tgctacaacc gaatcggaaa tcactgaaat gggactgcgg ttagcgggtg
     7861 ctggtaaaca gattggaatg agccaaggcg aaattctagg atttgctact gctctatcct
     7921 ctgttggtgt cgaagctgaa gcaggtgggt ctgccttttc gaaggtcatg atccagatgc
     7981 aactagctgt agaaaaagga tttggcgctt ttgatcagct gaaacagatg gcagaagaac
     8041 aaggtgttcc gtgggttaat ttagtaaatg ctgtgagaga cggtggaaaa tcgttaaaag
     8101 cagtatcaga gcaaatggga ttcacttcat ctgatttaaa gaaaatgtat aaagaagcgg
     8161 acaattcgaa aagttcgctt gaaaattttg cgaatgtggc aggtgttaca agtgaacaat
     8221 tccagaagtt gttcaagagt gatccttcag aagcaatcat caagttcatt caaggactga
     8281 aagattcaga aaaacatggg acttcagcaa tcaaagttct ggatgacatg gatataaaag
     8341 aagttcgact acgcgacagt ttattgagag ctgcaaatgc tagtgacata ttcaaaggag
     8401 caattgaaaa aggaaacggc gcgtggaaac aaaatacagc tttaacaaat gaagctaata
     8461 aaagatacga aacaactgaa tcaaagctaa agatgttgaa aaacgaagtg attgatacag
     8521 caatcgatct tggaggtcca tttgtagacg cgttgcgtga tggtctaaat gtatcaaaac
     8581 cgttgataaa aaacttaggg aatatggcga aagctttctc agccatgagt gatgatcaac
     8641 aaagaaacat tatcaaatgg gttggattag cagcagcagc tggtccagcg ctgaagcttt
     8701 taggaactgg tacttcagta ataggtaaag ttacttcaac tactggaaga ttgacgaaaa
     8761 gccttgttga tttagcagcg aaagcagcag aaaagaaggc tctggatgcc tttgcatcgt
     8821 cggtaacaac agctggtgct gcagctgcaa agacagcagg agctggaggt attggcgggt
     8881 tagtccctat gatgggaaaa acagctagtg ctgcaagtac tgctgcgggc gctagcggaa
     8941 taggcgcaat gacaacatca ctaggtttgt taggtcctgc tctacttggt attgttggcg
     9001 ttggtggcgc actagcagtt ggctatggag catggaaatt atttggtgaa gaagcttgga
     9061 attcctcgca aagagtaaag caatggggaa cagatgtcgg tgaggaaata gataatactc
     9121 ttgacggagt acagaagaat attgaaggtg caaatggtca attcggtctt ctcaaagagg
     9181 gttttactgt tgatgatgcg aataatatgg cggctaattt cgcaaccgct ggaacatctt
     9241 tggaaacatc tttaacaggt agaataaagg cattagacga tgcactaaaa gggctacctc
     9301 aatcagttca ggatgccatg aaagaagtag ctgaatcaga agaagaaacc ttagggcaat
     9361 ctctttctaa aataaaagac aacaatgaag aaataaaaaa aatcagagaa agagccgcaa
     9421 atcagaacag agacttgact gtttcagaac tacaacagat acaggatctg atgaaaggaa
     9481 cttctcaagc gtatgtggat acgctcgatg tttctgctaa agacagaaaa aagatactat
     9541 cagccatgaa tggtgatgtt gcacaggcta cagaagaaga agcgaagacg tggttacaat
     9601 ctttagcgaa acaaaaagct gcaactaccg aaaattatag acaaatgctt gaagaaaaaa
     9661 aggcggcttt aaaggaaaag ggagtaagca aagaattcat tgatgcatta gaagatgaaa
     9721 tgaatgatta catcaaaact acaaatcaag gattcgatag ccaaatagct gctataacaa
     9781 ataaatatcc acaattgatt aacgaagtga gtttatcaaa tggtcaatta tttagcaaaa
     9841 cggcagctgc tatggacaat actggtcagt atacaaaagt ttttctcgaa agtaatgaaa
     9901 agattttagc ccaagcgaag aaaacatcgg atgaaatagc agataatgcc gaaaaaaatg
     9961 cagaacaact tggtatggta gctgatgagg ctacgcatgc tggatcaact tggaatggat
    10021 tagttttaga cgaaaaaaca ggaaaagtaa aaactaacgt acaagaagta gtgaatgaag
    10081 caacgaagga tttcactaaa tggaatgaac ttaaacctgt tgttcatgat gcgaaactaa
    10141 caacgaacgc aaaagatgtc atttccatag cggcagtaca aaatggttat tgggatgata
    10201 tggaatggaa agaaaaagaa gcattattag aagatgagtg ttctaaaaat gtatacaagg
    10261 ctttagaaaa tgctggtaaa tggaatgagt tagatattcc ttctaaagaa gcaatcctaa
    10321 cttcaaatac tcctgaagtt atgggtgaaa ctctcatgaa tttgggacta tgggatactt
    10381 acaaacttca agtgaaagat ttagatttga atgcgtataa agtatacgat gctatcgatg
    10441 gatcagaaga aaaattaaat agttgggctc aattagataa ccccacaaaa gaattgttat
    10501 tagataactc agatttttct acaaaacttt ttaattccga agaaatgttt aaacgttgga
    10561 atcagttatc atctaatgca aagtttcttt cagctaataa tactaaattt acagaggttc
    10621 ttttatcatc tgacagaatg tggaatcaat gggtaaacct tcctgataat gagaaaagat
    10681 tacttgcaga taacgaagat ttagctacca ctgttttatc ttcagaagaa atatacggaa
    10741 gatggactca attaccagat aatcaaaaat atatgttagc tgacaatact gacttagcaa
    10801 acaaaatatt tgcttcagaa gaatcatgga atgcatggaa agcaattcca gacatagtta
    10861 aaaacttgct tggtaataat attgatttac tggctaaagt gaaagatggg actgtaagtg
    10921 tagaagaata taacaaagtt atgccacttc ttaaaactct atatggagat aacgttaatt
    10981 taaaagaggc aatcagtggg gctaaaagct ctatagagaa ttacaacaaa aatatttttc
    11041 ctaatgataa aactgcagta ggacacgatg aggcttcaca agctgctaaa gatgcatttg
    11101 aagcgttcaa catcttccaa acaaaaatac cagataaaat tactaaaact gtatcagctg
    11161 actttatagg tcctatgcct aatgctaaag gaacgaactt ccatcctggc ggagcagcta
    11221 tggtaaacga tcaaaaagga cctacttatg aagagcttat aaccttgcca agcggagaag
    11281 cttttatacc taaaggacgt aatgtcgttt tagacttacc aagaggttcg aaagtactga
    11341 acgccactaa aactaaacgt ctagtaccta aatatgctga tggtatagga aacataacga
    11401 cggtttcatc tacgctaaac ttagatgctt taattttagc tattaacgag ctaacgacgg
    11461 tattgagaac tcaactgatg gcagaaacga ctaatgctaa agatactaaa ctatcagatg
    11521 aaacaaaagt tgccgatccg attattccgg gcagtctttc agaaaaatca gatcagtatc
    11581 taggaattgg agctcaatgg ttaaccaatc taatgaatgg ttggaactca gcggttcctc
    11641 aatatatgaa tagtgaaatg gtttttatta caaactatct caatgcgcta aaacttcaaa
    11701 acaatcctaa ttatacgcaa ggagtagcat ggaataagaa cttactgaat ggatggaata
    11761 gtttaactgg gacatttatt tctacgatca attcattttg taatcaagca atggtcacgc
    11821 ttagaaacta caacactcct atgtacaaca atggccgaac gtggcagcaa aataatctta
    11881 atggttggaa ttcattgtat ggatcattca tagcccgtgt aaatcagctt ggtaacgatt
    11941 cgattaacaa ccttcgttct aaaagtggtg gtttttacaa tgcagggaca ttcttgttgc
    12001 aatctttaat aaacggaatg aattcgttag gtaattctct ttctacaaca atgaacaatg
    12061 tcgctaatac aatggttggt ggtatgggca aaggggttaa tggcgtcata tcgggagtta
    12121 actatgtgct taaggaagtc gagtcaagta agaatatcgg aaactggcca attccacaat
    12181 acgctaaggg aactgatgga catcctggtg gacttgctat gattaatgat caaaaaggcc
    12241 tagttcacga agaatatgta cagatgccag atggacgtgg ttttattgcc aaaggtagag
    12301 accttcttgt taatttgcct aaaggtgctc aggtcttaaa tgcttcttta acaaagaaat
    12361 taaaggaacg tttaaatatt ccacactacg aaaaaggtat tggcaattta gatattgttg
    12421 atttgttaga tgatgaaaaa agaatgttag aattcttgac tagcaaagtt gacttttcag
    12481 gcattaatga accatggctt gatatgacta aatcaggaac gtcattaatg tctaaagctg
    12541 caaatacaat gcttcaatca aaattaagtg aattcttcac tcatggcaat tttgatggtg
    12601 cagtaaatgc caatggtgtt tatcaatatt tagttgatgt tgcacagaaa gtaatgggta
    12661 agttccccgg acttacagta acttctggat atcgagcagg agatgcttac tatcacggaa
    12721 aacggcaagc tatagacttg gcttatccag gtatatctgg agatccgaga tatacagcag
    12781 cagctaatta cgcttttgaa aaattccctt caaaaattgc gtatgtcatt acgaatggac
    12841 gtgtacgtga ccgcatggga ttatctggaa cagggtcaag cggacaatgg acgaattggc
    12901 cagatggcga tcacttcgat catattcatt taaacggttc gatggggtct ggcgatatat
    12961 tcaatggagg agttggagga agtggtgttg cacgctggcg ttcttatgtg tctaaagcat
    13021 tgaaaatgaa tggattacca gctacagcag cttatataaa tgcctggatg tcccaaatcc
    13081 aaacggaatc tggtggtaat gagaaagcaa tcggcggcaa tgatgggctc gctgaaggca
    13141 atgcaacagg attacttcaa acgaaacccg gaacttttgc agctaacgct tttcctggtc
    13201 acggaaatat tatgaacgga tttgataaca tgttagcagc aatcaattat gctaagaaac
    13261 gttacggagt tgcaggtatg ttgcaagtta tcgggaaagg acatggatat gccaatggtg
    13321 gattgattac caaagacgga ctatatcgag cgggagaagg aaataaacca gaaatggtta
    13381 ttccattgac aagaaaaaca agagctattg aattgatggg tcaagcatta gcttttcttt
    13441 ctggcgatga caagaaacgc tccaacacta caaatacagc tgataattca gcagagcttg
    13501 taactttgat taaacagcaa caaaaacagc ataatgagtt aatgatgatt cttagagcga
    13561 ttcttggaaa agatttaagt ctcaagtctt ctgacattgg gcaagctgct aatcattata
    13621 tgggctctga tttgaataaa cttcgatata cgaatggagg tgtttgataa ttgttttaca
    13681 aattacagtt caatcaaaat gggaagttgt ttgatccgca aagaaaagaa aaaattgtat
    13741 gtaaagaaat caaacgtcaa gcaccagtgt atgaagtgaa ttacgaagat tttgaaggga
    13801 cgaacggtag cagagaagct aatgctagtt ttcgtccttt tgaattagtg ctgacttttg
    13861 atatctttta caaaaataag catgataaag aactgctatt aacagagttc tatgagctaa
    13921 tttttgtcgg ttatcaatat tacatttcct atgatttaag cccaggaaaa agatttaaag
    13981 taaatccaac aaatttcgaa attactgagg aagagaacga ttattctacg atagaaataa
    14041 cctttagtat tccatcaggg agtgctgaat ccagcgcaac cactttaacg gatttcaatc
    14101 tagaggaaga atggcaattc tcgcaaggtc tagtggcgga agattacaag tatacgcatc
    14161 agactagtca cttcattatt tacaatgctg gaagctttga aattgatcca cgtgaacatt
    14221 atctacggat cgcattagaa ggagaatcag aaggaaatgt gacgattttc aataaaacaa
    14281 ctggcgatcg atttatttac tatccatcac tttctacgaa tctcggacag acattagtct
    14341 tggatggcgt gattccaaaa ttgaacggtg taagttgtgg aatcaatacg aatcacggtc
    14401 tgatcaattt ggttgaggga gtcaatgaaa tcgagatcca aaatattact cgcgtgaaat
    14461 cttcatggga tttccgtttc ttatataagt aggtgattga gtgactgagt taattattcg
    14521 aaattatgaa caaaccaaag aagaaatcct tgtcggttat gacaagggtt ctttttatga
    14581 aaactggcaa caaaatgaaa cgtgggagat tggctttacc gttaccagca attcgttgaa
    14641 tcaagaagta tttgatttag tcgaatacga gtcttctgtt ttctacaacg gacaggaatt
    14701 tgtgatcaaa gaaatgactc gcaaagcact tggacaattg ctgacgaaac aagtagttgc
    14761 gacacatatc tattacaccg ttcaagatgg ttatcagtac aacacagtga caggtgcgaa
    14821 atctattagc cagttactca cgcatgtatt tagtgcaggg agtcgcggct ttacatggga
    14881 agtcattgat ccaaacaaaa aattccttac cgttgaacaa gaaaatttcg gtaacgcgaa
    14941 ttacttgaag ctgatcaatg agattctatc tgactataat gcagtcgtga ttccgaataa
    15001 taaacatcta actttctatc ctgccagtga gtacggccag cagacggaag aacagattcg
    15061 ctataaatac aatacagatg aagtgtcgtt tgatattgat acgtattcct tgaagacgca
    15121 gatcaaaggc tatggaaaat tgaaggatgg tgcaaatact gagaatccta aagatagtga
    15181 ctatatattt actcccatca cttatacaag ccctgaatca cagaagtggg gaatcaggat
    15241 acaagatcct gttaaagacg aacggtatac cgtatcaggg aacatgctcg agcggttaaa
    15301 gacagacttg caagactatc caaatatttc gggatccgta accttgaaat ggaaaatcag
    15361 tcccaacaaa ggcgatcacg tcccattcat ttatgaacct ttgaatatta atacgtacat
    15421 tcaagtggta ggaatcaaga cgtatccagc gataccaaat aagccaccag aaatcacatt
    15481 gagcaacaca aagaaaacaa tgacgtcgat actcgctgaa atggcgaaga aaggagtgat
    15541 ttgatggggt tattaaaatt aatcagtaac cgtatctcta cggaatggaa agagaaattt
    15601 aataaaaaca ttgactacct taatgatctt gaaaagaaac tgtctgatca agacaaatca
    15661 atgaacagtc gtattgataa tctcgtgctt cattcaggtg gcgaatcgcc gaatgaagtg
    15721 gttgatgctc gtgtaaataa taagggagaa acctttccta ctttacacgg cagattggta
    15781 gaacacgaaa ccctgacaga tgaacaaatt agcgaactga atacaaacat ggatagtcaa
    15841 aaagagcaag ttcaacaatt gaacaagtct gttcaacaaa ttattggtgg atacaatgag
    15901 ccaattaaca tctatgtttc taaagatggt agtgatattt tgggtgatgg atcagaagaa
    15961 aagcccttct taaccattca aacagccgta aataatgttc ctctaattac aacgtctcaa
    16021 attactattt gggtaggtag tggggcttac ttagaagata ttatgattag aaatttaaat
    16081 tttacatcat ttttaattcg acctatcgat aattttgaca ctattgatcc attaaaatca
    16141 gatttacctg ttaaaatacg atccatttgt ttcactgctt gcaaaggtta ttgtcaagtt
    16201 gctggaatgc aaattgtgga tacagcaaac ggagcagact atggaatcag aaatgagcaa
    16261 agtggctata tggcaatcaa tagatgtaaa ttcgcagaga acacaaaaac acttgcaaga
    16321 tataatgcag tttatgtcgg cggaacatca aagattaata tgtacggaga cacgacgttg
    16381 attaaccaga aagtggcaat ttacgctgta ttgatgggtg aaatttttgt cagtgcgttt
    16441 ggttcaggaa acgacgtagg aattttgtgc gagaatggca ctgtacgagg aactgtatca
    16501 acttcttttg ccacaacccc aacaaaaact actgggtacg gtctcatcat cacgaaaggc
    16561 acggtgttgc aataatggta tacaaaacaa atgaatcgat catcatgatt caagcagaag
    16621 ccactagtcc aaacaggacg aatgttgttt tttggtcgca tgatcgagga acagctaagc
    16681 ttcgaatgaa attagttcgg aaaaacggca ttcctcaaag cttacccgaa ggcacaactg
    16741 ttccgattcg cttgatgctc aaatctgcaa cggcagaagg tggttatgga aaacatgact
    16801 atctcgccac cattgaagat cgtgtaaatg gtattgtgtc cattgtgctg gaagataata
    16861 ttttaggcta tgttggaatt gtcgaaggta gtgtttatat tgatttccca aacgaccgct
    16921 cgttagatac agctggccgt tttacttttg acatcaaacg cagtccaatc gatgatagta
    16981 caccagaatt ggaagattat tatttcaatg gtttcagtca gactattgat aaaatcgaaa
    17041 aaattctagc tgatggaaag caagagattg aacagaaaat tacggaatct gaaacgcaga
    17101 ttgatgggaa actgaaagaa acaagcaaca aaatcacgaa agccaatcaa gatgtcgcaa
    17161 ctatcaatac gaatattgat aaagcaaatg atcgtattga tcaaacgaac cagcaaataa
    17221 gtgatctcgg aaagctgaaa aagatgtact ccaacagcat cgatttcggg aactatgatt
    17281 atagtggaaa tccgaatttg atggctaata taaacgctga tagcttctcg caaggtagtg
    17341 gtgccttatc tgttgtagat gatggtgacg aggtagtgat tacgcttgat ccaaaccata
    17401 aattagaaat attcaaagcg aaaagtcaac cagctctggt agtggggaaa acatatacca
    17461 tgagtgtaga aattatgtta gaagatgatt tcactggaga ccctagtaat atatctctaa
    17521 gatatattaa aatgcccagc tggttaacag tactatatac gccttataca ttaactaata
    17581 ctaagggtgt atggcaaaaa ctaactgtta ccgctaaaat gactactgcg attgataacg
    17641 ctgaaagctg gtttataatg ctacaaaata aagacgctaa taacagccta tccggcaaac
    17701 tacgtttgag acacgctaaa ctcgaagaag gctcaacagc tacaccattc caacctaact
    17761 tattagcaga accttacaac atgtgtcgcg aatatcctaa cgaaaatatt gccgatccta
    17821 aagttaagtt cccaatcgaa tctggcgacc accaaatata tcaaggctac acagaagaag
    17881 agcttatgat aggtcaaacg tatactatca cgcttaaagg aacaaaaccc gcaagtcaaa
    17941 cctttgtagc gtataatcat tggactgctc gtttaggaga actaaagccg gttgatgggt
    18001 tgacagacgt atggtctcta acattcacac caacgaatgt tgtggcgatg cctaaacttt
    18061 tccgtgttta tcagtatcca cgatcaacag taggcgcatg ccaaattgac tggctcaaga
    18121 ttgaaaaagg caacacccga acgcccaata ttagtcagtt taaatacttc ggtgaaggat
    18181 tgaaagacag caacaatccg aatgattaca gttgggatgt cacacctgaa tatactgaaa
    18241 aaggcttgaa taatgctgtt aatgtgtatg atcctcagag agttgaaggt ttgaaaaact
    18301 ttgccgatgg tattcaaata gcaggagata aagtgatcag tgaaaatgat tgcactgttt
    18361 atacattaac taaagacaac agtcaatcgt ttatcgatgg gtatgcaaca tttattaaac
    18421 acggaaaaga agttgttgta aatggtacag taaagttcaa aaaagcttat gcttttggtg
    18481 taccacttga tgatgaagtc ccagatgaat ttattgcaag aattgttcat ggcatgctta
    18541 caggtcagtc aggaacaaat agtgtatcaa aagcgatgta cgtgcaaaaa gacttaggaa
    18601 aaattatgac gaatagcgaa tttgccgcaa atgaatggtt tacattccac ggtaattatt
    18661 gggtaggagt gaaataaatg aaaaacattt ggaaatacgg acgtactggt ggagagtacg
    18721 caggaaaagt attggacgac atgcttgtat ccgttcctta cacggatcag ccaccgctcg
    18781 aaggattacg tgctgatggc gaaccgctaa cgattgctga tcagatgttt gatcctaaac
    18841 tgaaccaatg gattgtttta gcgaacacac tggatcacaa cgatttaaac aatctcaaag
    18901 cgatgtacga ggcactggaa cacgagaacg gcgatttaaa acagctcaac gccaaactca
    18961 tgctaagcga tgtagcaatt aaacaggaaa atactgcatt gaaagaaaaa gcggatagtt
    19021 tagcacaaat caattcaaaa atgatgcttg cttcgttaca aaatagcaaa gacatttcag
    19081 aaattaaaga gcaactaaat ccaactttaa agggaggtga gtagtatgtt tagttttagc
    19141 gatgtgaaaa tgatgtatga ttggggctgt tttactgacg atcaagttcg actattcgtt
    19201 ccactatgca ttacagacga agaagcagat aaaatcatta gcaaagaaga gagcgcatct
    19261 taagtgatgc gtttttttgt tggaaagttg gtggaacatg aaagaagaag cgctccaaga
    19321 cgttgtggag agattagtaa gaattgaaac aaaattagac aactacgaat cacttagaga
    19381 aaaggctgat agtgcaaaag atttagcaga taaagcctat tcagtagcac taaacaatgc
    19441 agaagacatc aaggaaatga agaacaataa taaatgggct tggggctata tgattggctt
    19501 aggcattacg atcattggct atttcttgac taaattgtaa aggaggtgag aagaaatgat
    19561 tttaccagat aagtattatc aagtcattaa atggacagtt ttaacagtat tgccagctgc
    19621 atctgcttta gtagccatgt tagggaaagc gtatggatgg aatggaacag atatgatagt
    19681 gttgactatc aatgcagtag caacattttt aggcgttatc actggtgtgt cggcttataa
    19741 tttgaaaaaa taggaggaaa caaatgaaaa agaaaattac tattactgcg atgagcctat
    19801 taacggctct ttttttattg ccaattaatg ggtttgccta tactatcaac aatgaattta
    19861 atttgggcgc aaatgaaggt agctcacaaa tagcaaataa tcagtacatt ttactgcatg
    19921 aaacggctaa tgaaacagca acaggacgca atgaagcgca gtatatgcaa cgttcatgga
    19981 ctagcgctta tactgcttat attgtgggag acggcggaat tgtttatcaa gtcggtcaac
    20041 ctggttatgt acagtacggt gctggttcgt atgctaatgc caacagtcct gtgcagattg
    20101 agttacaaca cacacatgat aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat
    20161 tggctagaga ttcagcaatg aaatatggta ttccattaac attagacacg ccttataacc
    20221 aaccaggaat caaatcacat ttatgggtaa cacaaaatat ctggggagat catactgatc
    20281 catacggtta tctttctgaa atgggcgtaa gtaaagaaaa attagc
//
LOCUS       DACDIH010000045        20039 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDIH010000045 DACDIH010000000
VERSION     DACDIH010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.045"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            160..1107
                     /locus_tag="IV281_001778"
     CDS             160..1107
                     /locus_tag="IV281_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HAP8780769.1"
                     /translation="MQGAVFELQNREGETLQTGLTTGEDGKLAIDGLAPGAYQLVETQ
                     APTGYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQ
                     DANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAV
                     VELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDG
                     LAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTS
                     SGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1531..1701)
                     /locus_tag="IV281_001779"
     CDS             complement(1531..1701)
                     /locus_tag="IV281_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8780770.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1895..2074)
                     /locus_tag="IV281_001780"
     CDS             complement(1895..2074)
                     /locus_tag="IV281_001780"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780771.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2461..3573
                     /locus_tag="IV281_001781"
     CDS             2461..3573
                     /locus_tag="IV281_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8780772.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3651..3944)
                     /locus_tag="IV281_001782"
     CDS             complement(3651..3944)
                     /locus_tag="IV281_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP8780773.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            4112..4426
                     /locus_tag="IV281_001783"
     CDS             4112..4426
                     /locus_tag="IV281_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP8780774.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4447..6426
                     /locus_tag="IV281_001784"
     CDS             4447..6426
                     /locus_tag="IV281_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8780775.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6439..6765
                     /locus_tag="IV281_001785"
     CDS             6439..6765
                     /locus_tag="IV281_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP8780776.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6781..7299
                     /locus_tag="IV281_001786"
     CDS             6781..7299
                     /locus_tag="IV281_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780777.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7299..8651
                     /gene="celB"
                     /locus_tag="IV281_001787"
     CDS             7299..8651
                     /gene="celB"
                     /locus_tag="IV281_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8780778.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8735..10240)
                     /locus_tag="IV281_001788"
     CDS             complement(8735..10240)
                     /locus_tag="IV281_001788"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP8780779.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10332..10922)
                     /locus_tag="IV281_001789"
     CDS             complement(10332..10922)
                     /locus_tag="IV281_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8780780.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            11101..12749
                     /locus_tag="IV281_001790"
                     /pseudo
     CDS             11101..12749
                     /locus_tag="IV281_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
     gene            12798..13517
                     /locus_tag="IV281_001791"
     CDS             12798..13517
                     /locus_tag="IV281_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8780781.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            14116..15063
                     /locus_tag="IV281_001792"
     CDS             14116..15063
                     /locus_tag="IV281_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8780782.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            15075..15995
                     /locus_tag="IV281_001793"
     CDS             15075..15995
                     /locus_tag="IV281_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8780783.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            16036..17493
                     /locus_tag="IV281_001794"
     CDS             16036..17493
                     /locus_tag="IV281_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8780784.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17838..18452
                     /locus_tag="IV281_001795"
     CDS             17838..18452
                     /locus_tag="IV281_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8780785.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18445..>20039
                     /locus_tag="IV281_001796"
     CDS             18445..>20039
                     /locus_tag="IV281_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8780786.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVEL"
BASE COUNT         6388 a         3430 c         4065 g         6156 t
ORIGIN      
        1 gtagaaactc aagcgccaat agggtatgaa ttagatgcga caccaatcga gtttgagatc
       61 gaaagaagtc agacagcggt ggtagaattg acgaaagaaa accgacttac accaggcggt
      121 gtcgtgttga caaagatcga tgaccaaagc ggggaaatcc tgcaaggagc tgtttttgag
      181 ttacagaacc gagaaggaga aacgctacaa accggtctaa ctacaggaga agatggaaaa
      241 ctggcaattg acggtttagc accaggagca tatcagctgg tagaaactca agcgccaacg
      301 gggtatgaat tagatgcgac accgatcgag tttgagatcg aaagaagtca gacagcggtg
      361 gtagaattga cgaaagcaaa ccgacttaca ccaggcggtg tcgtgttgac aaagatcgat
      421 gaccaaagcg gggaagtcct gcaaggagct gtttttgagt tacaagatgc caatggaacc
      481 gttctgcagt caggtctaac cacaggagca gatggaaaac tggcgattga cggcttagca
      541 ccaggagtgt atcagctggt agaaacccaa gcgccaacgg ggtatgaatt ggatgcgaca
      601 ccaatcgagt ttgagatcga aagaagtcag acagcggtgg tagaattgac gaaagaaaat
      661 cgacttacac caggcggtgt cgtgttgaca aagatcgatg accaaagcgg agaagtcctg
      721 caaggagctg tttttgagtt acaagatgcc aatggaaccg ttctgcagtc aggtctaacc
      781 acaggagcag atggaaaact ggcgatagat ggcttagcac caggagcata tcagctggta
      841 gaaactcaag cgccaacggg gtatgaattg gatgcgacac cagtaacttt caagatcgaa
      901 aaagaacaag aagtggcgat ctttttaaca aaagagaata gaaaagttgc tgatagctca
      961 gatataagag gaaacaatcc aacaagcagt ggaaataagc atcttccaaa aactggagaa
     1021 accatcatta gccagtttat attgtcaata ttgggtgtgc tacttgtttt catcagtata
     1081 aaatttagaa agaaacgaaa tatatgagct aaaataaaaa attgaataag catagttttt
     1141 aaaatcaact agtacccatc aattgaatgg aaactaccga ttttagaagg ggtatacaaa
     1201 caaatattag tatcggagta gagaaggttg aacccaacca gtaaataagt aaagacaatt
     1261 ttgtgctgcg ttgagaagtg aaaaataaac ctttgtagta atactagtta ataaaaaata
     1321 tacaaaaaca ctgcgaaaat cattcttgag tttcgcagtg ttttttgcct aatgactcaa
     1381 gtttattggg taatactttg attttctttc tacaaataat cagcgatcag ttgtaatttt
     1441 ttctctagtt ttaatggctc tttgtcaata aggagaaaaa caagtcgggt cttttgtctc
     1501 ggaaagcttc aaccaactcg tgaataaagg ttacgcctcc ttgaaaggcg tggaaaaacc
     1561 aagtaatcga tcaatcacca atttcgcatt tggtagaaca tgtttggtga gctgaaagta
     1621 ggtggcgttc atgtctgtaa ctaagaattc tatttcttta ggattagtgc agctaaggaa
     1681 atagtttgtt aatcgaggaa ttttttgtgt aggtaaattt tctcgattaa tttttctgtc
     1741 tcgccatccg cggaaataaa actcagttta tcctcaacgg aaacgtgaga acgaaattca
     1801 caaccctcaa cactcaagac agaattcttt tggaagactt tggtaaatag cttctcaact
     1861 ctttcaatgt gcgaataacg gttgtcaaag atacctaaca acttttcgaa ataaaaaata
     1921 acgacacttt ttcagtcagt aaagaagtga ttttatgtct gacatgattt gaaatcgagt
     1981 gccttggacg gataaaataa ctttgagcgt tccaatggct tcgacagttt ttacaggtat
     2041 agcgctgctt ttttaggcgt ataaccattg gcatatgatt atattattca aagtggacaa
     2101 tggtttcctt cttcttgttt ttcaccacca ttaatttcct atttccatct accacagcag
     2161 aaccacaatt tctacagaca catggagcag ggaagagaac tgcagcaacg atcaatgttt
     2221 ttttcttctg taaggtttcg taagagacct ctgtaatcat caaatttttg tctattgatc
     2281 tcagaatttt tttgatagaa ccattcataa agcatatcgt tctctcagtt gtttattttg
     2341 tggtgattta atcatacaag aaaacgatat gtttttttat aaaaacatga ataggaaaaa
     2401 tagttgacaa gaaaatgaca acgccatatg atgttccata gtatttacaa cctgtcatag
     2461 atgactgtaa aaaataaaag taaaaagaaa ggacgacaac aggtggattt ttatcagtct
     2521 ctacaactag atccgtttat tttaaaacaa aaaatacgag aagcagcttc taaaaaagaa
     2581 aagtgcatgt atatttgttc attgttttta cgatcattat tcatcgtatt gtttgccatt
     2641 tgttttatca ttataattac aacattattt gaatcgaagc ataaaccata cgctgtggtt
     2701 ttattctgta tgttgatgag tattcgtttt gttgactttg gttataagat ttcccattcg
     2761 atcataggtt tagcaatcgt catgttctca ctattagtag caccgtatgt ccagctgatc
     2821 aaatggtcag ttatgggcat gttgattcat tttattttac taagcagcat attaatggca
     2881 accgctagtg atcccaaaat ggggaatgct tctttgtacg gcttttcata tctctttatt
     2941 gtttattcgt tacctaaaga ttcgttgaac aagaatttct ttacacaaac aagtagctta
     3001 ttatttttgt ttttttgttg gttttcagtt ctcctttaca gaaaacatcg agaaaagaac
     3061 aaagacaaaa gcttgtttaa agagattttt ttgaaaggca tgtattcgca ggaaaaaatt
     3121 tggatgctca tctatgcatt tggcattagt cttttgatcg ttgcaggaga gtatgtccct
     3181 tttcagcgat tgatgtgggc agggtttgca ttttcttcta ttgtatctag ctatggattg
     3241 atgtcgattg gtttcaaaga acgagcagta gaccgaatca ttggctcatt gattggatgt
     3301 gttttattta ttggtatcag ccaatttatt ccttttaact gggtcggtat attaggtggt
     3361 ctagctttag gtgtttgttc tacctataga tataaaacag tttttaattc ttttggtgct
     3421 ttggcaatca ctgccagttt attcggggtt ccaggagcag taacgatccg catttttgag
     3481 aatatccttg gggtatgttt aggaatcatg tatattgggg tgactgaaat acttatccga
     3541 aaaataagag aaaaacacgg tttgaatcat tagatgagat cgaataaaaa ataaggaaaa
     3601 agtgaagata cacatggatg ataaattgct atcattccat gtgtatcttt ttacggtaaa
     3661 tcagtgaaac gttcgacttt catttttaat ttatactttt tacggagttc gtttatttga
     3721 gccatcattt cggatttgtg atgcttttcg attgcttctt catctttcca acggtcgatc
     3781 agtaaaagag attctggatc ttctagtgga aagtaatagt catatttctc gtttccttct
     3841 tctgcacgaa tcgctttgac aatgtttctt tcgaccattt cttctgcaaa ttttctggct
     3901 tgtccatttg tacttgtata caaaatattc actgtgatac tcactaaatc ccctcctgaa
     3961 aaaattctat cactagtata tcacggtgcc agaaattatt attcaagata tgatacacat
     4021 tttttccgca atgctacgga aatatatatt ttgaatagaa aacgcttaca agatatgctt
     4081 ggtttataaa aataaaagga gtgattgatt tatgaaaaaa gcattgatta tttgtgcggc
     4141 aggaatgtca tcctcgatga tggcctcaaa gacaacggat ttctttaagc aaaaaagaga
     4201 agaggtactc gtagatgcag tttcagctac agaaggcgat aatatgatta agaatagtga
     4261 ttttgattta tttctgatca gcccgcaaac gacgatgttt ttggataaat tcaaaaaatt
     4321 aggagaatct gttggaaagc ctgttgttag tatccctttc caagcgtatg tcccaatccc
     4381 tacaggcatc cagaagttag cagaggtgat cgaagagaac atatgatgga ataggagtgg
     4441 atgataatga aacagaagga aaaattgctg ttgagatatt tgattgatca caagaaggag
     4501 tatgtcacca gtcaacgttt agcttctgaa ttgttattat ctgatcggac aatccgtaat
     4561 tacttgcagc ggatcaaaga gttagtagaa aaaaacggag gtaagatcat tgctaaacca
     4621 ggttatggct accaactgca tattttacaa agactgacat ttgacttgtt tttgagtcgg
     4681 caagaaatag tacctggata tgctagagaa ccgcaaggat tttatgagtc cgaagatcga
     4741 caaaagtata tattgaacca attgcttttg gaagatgcca ttctttttat ggatgatttg
     4801 gcagaagagc tttatatcag tcgttccagt ttgacaaagg acatgcaaga aatcaaggag
     4861 cgattgattc catattcact aaaaatcgtt tctaagcatg gtcaagggac atggatcgaa
     4921 ggggaagaaa gaaatcgacg gcattttatc atggatactt tttttggaaa ccattatgga
     4981 aattcattga aagaatattt agggcaaagc cgttttttcc cagacatcag ttttgaagag
     5041 ctagtcatta tcattctcga tgaaacaaga gaagctaagt taaaagtttc ggattttatc
     5101 atacagaatc tgactttaca cttggctttg gggatcaaac ggctccgaga agggtttgaa
     5161 atcaaggaat taggcatcga acaagaagtt tctaagcgag tagagtatca agtggcgcag
     5221 cggatcgttc aacgtattga ggtaatcgca ggtgtatcgt ttcccgaaga agaaattgct
     5281 tatttgacat tacatctcat ggctaagtcg aatcacaaag aaaatcaaaa tgatcaggaa
     5341 ctatcaaagg aattagcgaa agttctgaaa agattgtctc aggaatttca tttttcatta
     5401 gtagaggatc tccagctttt caatggacta ctcgatcatt tgtcaccgat gatgattcga
     5461 ttgagccgag gtattcgcat tgagaaccca ttgactgagg agataaagaa agaaaatccc
     5521 aaagtctttg acgcagtaaa aaggcatttc agcaacatgc cggctttgaa aaactatacc
     5581 atcaatgaag atgaatgggc ctatttggct ttgcatctca tggcggcatt ggaaaaagaa
     5641 agagctgcgc ataaactaca tgcattgatt atctgtgcaa cgggttacgg cagtgcccaa
     5701 ctattaaaga atcgcgtagt cagtgaattc ggtaaaaata ttacagtggt aagtgtaaaa
     5761 ggctattatg agatcaatga aacaacttta aaaggaatcg acctgattat ttcttctatt
     5821 gatctttcga caatgttttt caaaattcca gttttacatg tcagtatttt tttgaatgaa
     5881 gatgatgttc gtaaaatacg aaaggtagtt ggagaatcta taccctatca ttcctcagat
     5941 agaccgatac cagcattttc tctacagcaa aaaaagcaga tttacaccga acagttgtct
     6001 gaaaaatttt tcaagactta tacgtacgaa ccgaaaaagg aagaaattct tcatgatttg
     6061 ctgcaaaagc tgagcataaa tgaagaagaa ggatatataa gagaaatgcg aaagcagatc
     6121 cagcaacgtg aaaatatggg acaaatcata ttcagtgata cagtggcagt cccacatccc
     6181 gctgttccag ttggaatcga aacaaaggta gccgttgcat tgattccttc tgggatgcag
     6241 tgggatcaat atcaagaaat tcattttgtt tttttagttt cgccttctta tgtcgaaaat
     6301 gaaggaatta cggtcgtgac taaagcaatc gttaggttag ttgaccagct aaaagtacag
     6361 caggaaatct tagctgaacc tatttttgag aatttcagca gtaaatttat caaactgatc
     6421 gaataaggag gaaaacagat ggctgaaaaa ataaacagtg aaacactaca agtgacttct
     6481 tttgatatca ttttccatag cggaaatgca cggacactga tacatgaagc atttcgctcg
     6541 atgcgtaaag agatgtttga agaagtagaa gagaagttgc gacaagcaaa tgaagagatt
     6601 cttcatgcgc atcagtcaca aactcaactt ctgaaagaat atgccagcgg gacaaaaata
     6661 gaaatggaaa ttatcatggt ccatgcacaa gatcatctga tgacgacgat gacattattg
     6721 gaagttgcac aggaaatgaa ttatttgtat caaaaaaata aatagaaagg aaggatcatt
     6781 gtgcaggata cagtaaaaag gaaaatcgaa ttagaaaaaa agcaacttag tttgaaaaac
     6841 atgtatttca atcgttatct aatcgtacgt tacctgactg cctttttctt tttcatgaac
     6901 atgcaatgga tgattctgct tctatcagct aaaagtttag gaagtagttt accgatgatt
     6961 ctgattttag ctattttgcc tgcagtagga gaacaagtaa agctttatcg aaaacaccaa
     7021 acgaacgttc catggacaaa gtggtatttt ttgttccaga gtgtctttaa tttattagtg
     7081 attccagtgt tgtttacctc aggatttaca ttcttgtatc cttttatggc aaataacaat
     7141 ggaggacagt tatttgtttt cattcttgtt gtaagcggta tttttgccag tgcatttata
     7201 cagtatcgtt taaagaaaat ttcactcaac caagaccaac agtatatccg tattaaacaa
     7261 tatgaaaaag cgttatattt aggaaaggag aataactaat gtttgatttt ttgcagaaat
     7321 acctgatggg acctatgggg aaaattgcgc aacttaaaat cgttcgggct gtcatggcag
     7381 cggggatggc ttctattcct ttcacgattg taggctcgat gtttttggtt ttaaatattt
     7441 tgccattacc gttcccattt ttagaaggat tttttaatgc aacttttttc aaagttagtg
     7501 acttgtacat gatcgtcaat acaatgacga tggggatttt atctatttac tttgccattg
     7561 tgtttgctta tgaattaacg tcaattgaac gagatgaaca aaaattaaat gttaatccgc
     7621 tcacgggggc attactatct gtttttgcat tctttatgtg tatccctgaa ttgatcatga
     7681 ctgatggtaa aatcagcttg attgcttcta tcaatgagac agaaactgta gtaaatggta
     7741 ttaggatggg ctcttttgtc gaacgtttag gaacttcagg aatctttaca gctattatta
     7801 tgtcgattat tgcggtcgaa ttgtattgta tgtgtgtcaa gagaaattgg gtcataaaaa
     7861 tgccagatgt tgttccgcca ggagtttctc gttctttcac tgctctgatc ccaacatttg
     7921 tcattgcgtt tgtggtgatg atcatcaatg gattattagt tgcactagga acggatatct
     7981 tcaaaatgat tgctatccca tttgggtttg ttacgcagtt gacgaataca tggcttggtg
     8041 tcatggtgat ttatttcttg atccatgcat tgtggatcgt agggatccat ggagcgaata
     8101 tcatcacttc tttcttgacc ccgatcgtat tagctaatat ggcagctaac gcacaaggcg
     8161 caaattatcc gttggcagga gaattcaaca attcttatgt aaccgttggt ggatcaggtg
     8221 cgactttagg attgatcatc tttattgcat tcctcgcgaa atctgatcag ctgaaagtgt
     8281 taggaaaagc ctcacttgta ccaggattat tcaatatcaa tgaaccaatc attttcggga
     8341 tgccaatcgt ctacaatccc tatttagcgg ttccgttttt cttagctccg atggcttctg
     8401 ctacactcgc ttattttgcc attcggctgg aaatcgtcaa accgatgctt gcacagatgc
     8461 cgtggccttc accagtcggg atcggagcat ttgttggtag tggtggtgat tggaaagctg
     8521 ctgtgttagc agtcatttgt gcaatcatcg cattcattat ctggttccca tttatcaaat
     8581 tctatgataa taaattatta aatgaagaaa gagaaaaggc agcagaatta gctagtgaaa
     8641 agttaaaatg atttctaaga aaaaaggaac tgtgacaaaa aatcacagtc cctttttttc
     8701 ttagaaatgg gaaaccacta gtatataaat accattattc ttcaatcaac cgcttcacga
     8761 ttgcataatc cccttcataa ggttcatcta caccattgat tttttgatag ggatccacgc
     8821 cttttcccgc aataacgact gaatcttgcg gagcgctttc tgctaaagcg cgtcgaatcg
     8881 cttcaggacg atccatttca taatgaacga tcaaatctgg attagtaatt gcttcattga
     8941 tttcttccgc gatttttttc ggatcttcaa atgctgggtc atctgccgtc aaaaaggcga
     9001 tgtcagctgt ttctgatagc acttttccaa aatcatgtcg acgggagatt gctttgtttc
     9061 ctgggctgcc taatacaaca attacacggc catctggatg ttcatttttg gcaaatttca
     9121 gcaatgtttt caagcttaga taattatgtg catagtccac atagacatgc gctccattgg
     9181 attggatcaa ttgatccatt cggcctggta cacgagcttc ttttaatccg tcttgtgcat
     9241 cttgactgga ggcacctgtt agtgctgcgg caatgatcgc actggctgca ttttcttggt
     9301 tgaatcctcc agccagcaaa atatcataag cagtatcgca gatagctaag cgatcttcat
     9361 gagaagataa agtaaaaccg tgagtacctt tatctgaacg gatcacttga taattgcttt
     9421 cttccgaacg accataagtg atcgttggaa tctggaatag atcggctgtt tccctaagta
     9481 aatcaaaata gtcactttca tgatttaaaa caactgtttt tgaattcaag atcaattgtc
     9541 gcttgcaata gaaatagtca tcaaatgtcg gatgttcgat cggactgata tggtcaggcg
     9601 agatattcaa gaagattccg acatctaaag tcaagccgta tacgcgttgt gttttgtaag
     9661 cttgtgaaga aacttccatc actagatgag tcataccatt ttcaactgct tctgacatca
     9721 tacgatataa atctaatgac tcaggcgttg tcaaatggga tttgaaatat gttttgccat
     9781 ctaacgttgt attcatagta gaaagcaatg ctgttttttt atttgttgtg tgatccaaga
     9841 ttgcttttgt aaagtacgca gcagttgttt taccttttgt tccagtgaat ccgatcactt
     9901 tcaatttatt ttgcggatag tcgtaaaaag ccatagcaag aagcgccatt gctttacgga
     9961 tatccgttac gatgattcct actgctgaat tttcgtatgg ttgttctgat acataatatt
    10021 gaataccctg ttcgattgct gaatccaaat attcttcttt gaaattcatt cctttacaga
    10081 aaaacaaagt agaagcatca gcttttcttg aatcataact taactgaaga aattcagctt
    10141 tttctggtac atcaagatgc cagcctcttg ttgaaacaaa ttcttttaat agattttctt
    10201 ttaatagtaa atgacggatg tcttctaaag agaagctcat tgattgatcc ctcgctttat
    10261 tcaatttctt ggtttattat agcacactct ttttaatata tcgtttgttt ccgctatgac
    10321 aaaatccagc tttattcaaa ttttcttgac acaggggaca agcggatgag tattttcata
    10381 aacaatgaac tgactgtaat agataggaga acgaatagtg tctccaacaa tccaaaaaca
    10441 taataattca aaaagacgat aagactattg gtaagtaaaa gtccttgcgg cattggaagc
    10501 cgaaaaaatt tgtggaaaat aatcggcggt atcgttatac cgccatttga ggccccgact
    10561 gtgtacaatg tgtaaacccc aagactgaac aaaagacttc ctgaaatcag tgaaaagaaa
    10621 tgtgaggaga tcatttccac ttttggaaca atctctaaaa caacaggaaa aagcagactt
    10681 cccgctacag tcttaaaaaa tactgatttt cctaatagga cagcggctag aagcagcatc
    10741 ataagattga tgctccaaac agtaactgag cgttggatag aaaaggccgt ttcaactaaa
    10801 tgtccgatgg cgcccactcc ggcggaagct atatcgtgag ggagcaagaa ccaattcaat
    10861 ccaactgtga taaagaaaag acctaaaaat ataacgggta gtttttttat ttcttgtgtc
    10921 attgatatct cctataaatg ttagccataa aaggctgtat atttctttac tttaccaaaa
    10981 aaacgaaacc gaattcaaaa aaacaattgt tcttataaaa gttatataaa atcgattgcc
    11041 agtttctgtt ttaccaatta aaatgatagt aatgttttgt agaaagttgg aatctgaccg
    11101 atggataatc aaagaaaacc agcattagat caattaacac cgcaagaaaa aatggcacga
    11161 ggctctgctt ggatgaccgc cagtaatatc atttcaagat tgctaggcgc tgtctatatc
    11221 attccctggt atgcttggat gggcgaaaat gctaaggctg ccaatggatt attcaatatg
    11281 ggatacaata tatatgcatt gtttctactg atctctacag caggaatacc tgctgcgatt
    11341 gcaaaacaaa ctgcacgcta taattcattg aatgaatatg gcacttcacg acgattattt
    11401 atccgtgcat tacagatgat gggcgggtta ggcctgttgt ttgctttgtt catgtacatc
    11461 gcctctcctt ggctagctca tgcttctggt ggcggagaag aactgattcc aacgatgcgt
    11521 tctttgagta tcgctgtatt ggtttttcct tgtatgagtg tgatacgagg gtattttcaa
    11581 ggcaatcaag aaatgatgcc ttatgcgctg tcgcagattg tagaacaggt ggcacgtatt
    11641 ttttatatgc tattggctac attcatcatc atgaaagtca tggatggtga ctatgtgacg
    11701 gcggtcacgc aatcaacttt cgcggcattt atcggtatgc tagcaagtat tcttgtcctt
    11761 ttgtattttt tgaagaaaca tcaagcgtat acatcggcgt ttattcacta cagcgaaaac
    11821 aaaataaata ttgcaacgaa ggaattgttg ttagataccg ttaaagaagc gattccgttt
    11881 attattgtgg gttcaggggt aacgatcttc aaattagtcg atcagttcac ttttatgaag
    11941 atcatgtctg catccaccga gtattccaat gcacagttac tggatctttt ttcgattttt
    12001 agtgccaatc cagataagtt gacgatggta gttattgcat tggcaacatc gatcgcgtct
    12061 actgggttac cgttgattac agaagctgtt acgttgaaag atcgagtagg tctggcaaaa
    12121 ttgacaagcg gaaatttaca gttgttttca ttttttatgt ttccggcgac atttggtgtt
    12181 atgttacttg cctatccgct aaatacatta ttttatacac cggattctct aggaagtaat
    12241 gtactgatcc aagcaagttt tgtaggactc ttcctaggac tatatatgct agtttccaac
    12301 atgctgcaag gaatgtttga aaacaaggcg gcaattaatt atttggttat tgggtttcta
    12361 gtgaaactcc ttcttcaata cccagccatt cgtttatttg aagtatacgg ccctttattg
    12421 gcgacaatga tcggatttgc tgtgtcttgt accttgatct taaagagaat ccatgaagta
    12481 gcccgcttta aaccaagttt tgtctggcgc aggacacatt gctgatcttt attcttacct
    12541 tgattatgct tttggcttcg tggatcacaa agatgatttt tggtgctttt ttaaacgaag
    12601 atagcaaatt ccagtctcta ttgttgattc ttgtagtagc aggtataggg gctttagtgt
    12661 atggttattt agctttgaaa atccgcttgg cagaaagact actaggacca ggaatagcgc
    12721 gtttacgaca taaattaaaa ataaaataag tgaatcagaa gagctagatt gatgctcttt
    12781 tgaaatgggg gaaacgcatg cgtttagata agtttttagc agatatggga cttggcagcc
    12841 gcaaagaagt caaaggatta atcaaaaaag gaacgatcga agtcaatgga caagtggtga
    12901 aaagcgataa agcgcaagta aatgaattcg aagatgcggt tacttattta ggagaaatca
    12961 ttacgtatca aaaagatttt tactacattt tgcataaacc tgcaggagtc atttctgcta
    13021 ctcaggataa tcatgatcaa accgtaatcg atttattgga agaccaggat tatcgggaag
    13081 atctttttcc agtcggccga ttagataaag acacagaggg cctcttgatt ttaacgaatg
    13141 acggggtgtt tgcccatcag ttactatcac ctaaaaaaca tgtggagaaa gaatatctag
    13201 ctgaggttag cggtataatg acagaagaag atatccgttt atttgcagaa ggactgatta
    13261 ttgatggcga tgagcagaca ttgccggcaa aactccaaat tgatcaagta gatcaagaga
    13321 cagaaacttc aattatccgt ctgattttgc atgaaggaaa gtttcaccaa gttaagcgaa
    13381 tggtcaaagc agtaggaaaa gaagtcttat atttaaaaag aatccgtatg ggaaaatttt
    13441 ggctgcctaa agatttgaat aaagggcaat atcgacccat gaccaaagaa gagctagaac
    13501 aagtgagaga aaaataattg aatggatagc taaaagaata agcagagtag ttgtagcgcc
    13561 atctgaaaag cagacttgtc cgtctgactt tcggatggct tttttgtgaa atttcggctt
    13621 atttccgtag aagctacttc tggaacaccg tttgttcgga ataagggact gtgacaagac
    13681 ttttgttaca gtccccattg ttccttgtga ctcagagtta gatagtaagg acacgatctc
    13741 cctaatactc aggccaaaac gctgaattca caacctctga taaacggtgt tcaatcagct
    13801 gaccctcggt attagctcaa aaaacggcaa aatatgagga actattttcc cgtttttttc
    13861 tctaatactc aggccaaaac gctgatttca caacctctga taaacggtgt tcaatcagct
    13921 gaccctcggt attagctcaa aaaacggcaa aatatgagga actattttcc cgtttttttc
    13981 tctaatactc aggccaaaac gctgatttca caacctctcc tatactttgt ttcattttcc
    14041 ctatactttg tttatttatt ctcattattg aaagcgctat aattacgttg cgaatgaatc
    14101 gaaaggggtt tttagatgaa aaaacgaaag gggttcaatc atttttttat gaatctatgg
    14161 cgttataaag cacttgtcct gatggctgta ccgggtatgc tttggatgat ttttttcttt
    14221 tacatacctg taatggcgaa tgtcgtagca ttcaaaaatt ttcatatttc agcagatggg
    14281 ttcttggcta gcttgaggga aagtccttgg gtgggttggg aaaatttcaa atttttattt
    14341 tcttcggatc aagcatttct catcactaaa aatacaattt tatataatgt agcatttatc
    14401 cttttgaatc tgctgatctc agtcgtattt gcaatcatta tgagcgagtt gagaaataaa
    14461 cgactcgtta aagtttatca aacgatgtcc ttactacctt attttctatc ttgggtcatt
    14521 atcagttact ttgtttacgc ttttttaagt cctgacaaag gaatattcaa tcagtggatc
    14581 actggacatg gaggagaagc tgtcaattgg tataatgaac cgaaatattg gccgtttatc
    14641 ttggtgttca ttggtacatg gaaagggatt ggttataaca gcattattta ctttgcatct
    14701 gttatgggaa tcgatccgac ttattatgaa gctgctatgg ttgatggagc tagcaaatgg
    14761 cagcagatca aacatgtgac gataccacaa cttattccac taatgacgat tttaacgatt
    14821 ttagccgtag gaaacatttt ccgagctgat tttggcctgt tttataacat tcctaggaat
    14881 tccggtgcat tatatgatgt gactcaagtt ttggatacgt atatctacaa tggcctgaca
    14941 tccacaggag attttggtat gacggctgct gcaggacttt accaatcagt tgtcggcttt
    15001 gtcttgttga tgatcacaaa tacgatcgcc cgacgttttg atagcgattc tgcattattc
    15061 taaggaggaa aaaaatgaaa aaaaagaaag tggagagagt ccaagtccgc tctttcaata
    15121 agacggctaa ttttttctct aatataatga ttgcactctt tgctatttcc tgtattttgc
    15181 catttatatt tgttatcgcc atctctttta ccgatgagtc catattgtcg actcacgggt
    15241 acagtttttg gccagaaaag ttcagtatat ttggttatac gtacttgttc gaccagatgc
    15301 aggataaaat tttccaagcg cttttcgtca ctgtacttgt tactgtttta ggtacactga
    15361 tcaatagcac ggctacttct ttatatgctt atgcgatttc acggtcaaac tttccattcc
    15421 gtcgtttttt taccgttttt tgtttgatta cgatgttgtt ctcaccaggg atggtggcca
    15481 attatcttgt tatgacaaat cttcttcagt taaaagatac gatttgggca ttgatcttgc
    15541 caatggctgt cagtcccttc aatattattg tgatgcgcac attctttaaa cgttcggtct
    15601 ctgattcgat tattgaatcg gcacggatcg atggagctag cgaactgcga atatttgttc
    15661 agatcgtttt accgttagct gttccaggta ttgcgacaat cagtttattt gccgcattgg
    15721 gttactggaa tgactggttc aatgctttac tatacatcca agatgataag ctagttccac
    15781 tacagtatct tttgatgaaa atccaaagta atattcaata tctgacacaa aattcaggag
    15841 caggaagcca attgacaggc ggattagcct cagttccagg agaatctgcc agaatggcga
    15901 tcgtagtcat ctcgacgttg cctattgcga tcagctatcc atttttccag aagcattttg
    15961 tcaaaggtct aacaatcggc ggagtaaaag aataaagaaa caaaaatata aatcataaaa
    16021 ttaggaggaa gaaaaatgaa gttatggaag aaactagcct ttacaggtgt ttcagcgttg
    16081 ttgttgggaa cattagcagc atgcggaggt tctggttcta aagaccaggc agaagctagc
    16141 aattcggaca ctttgcagat gtatcaaatc ggagataaac cagataattt cgatcaattg
    16201 atggaagtag caaacaaacg aatcgaaaaa gagatcggtg taaaagtcaa tatcaattac
    16261 attggctggg gcgattacga aaagaaaatg aacgtcatca tttcttccgg ggaaaattat
    16321 gatatcgcct ttgcgaacaa ctatgtttcc aatgcccaaa aaggagcctt tgctgatttg
    16381 actgaattag cgcctaagta cgctgaaaaa gcttataatg atttagatga agcatatatt
    16441 aaaggaaatc tggtcaatgg aaaattatat gctttcccag tcaatggaaa tgtctttgct
    16501 caacaagtac ttacttttaa caaagcttta ctagataaat acgatctttc aattgatgga
    16561 atccaatctt acgctgatgc agaaaaagtc ttgaaggaat tccatgaaaa agaaccaaac
    16621 acggcagctt ttgccattgg acaaggcttc aaagtccaag ggaactttga ttatccttta
    16681 gggaatacgt taccatttgc tattgatctg gatggagatg attcgaaaat catcaaccaa
    16741 tacgataatg aaacgtatat cggactgtta cgtacaatgc atagctggta caaacaagga
    16801 ctcattccaa gtgacgctgc aacgagcaat aaggattatc cactagaagg aaatacttgg
    16861 ctaatgcgtg gagaaacaca aggaccgtat gattatggcg atacaatttt gacaaatgca
    16921 gcaaaacaag aacttgtctc aaaagcaatt actgttccac taaaatctac agctcaagca
    16981 caaatggcaa attttgtcgt atcaaatact tcaaaaaata aagaaaaatc agtagaactt
    17041 ctagggctgc ttaacagtga tccagaatta ttgaatgggc ttgtttgggg gatcgaggga
    17101 gaagcatggg aaaaagtcga tggttcagat gataaaatca aattgctaga tggttatcag
    17161 ccaaatacac atatgtctgc ttggaataca ggaaacaaca agattctata tacacaagag
    17221 tctattacgg atgacatgat tgccaaacgt gatcaatcga ttgctgatgc agaaacttca
    17281 ccgattttag gattcagctt caacactgac agtgtcaaaa cagaattaag taatatttct
    17341 aatgtcatga atcaatattt agatggattg aatacaggga ctgttgatcc agatgaaaca
    17401 ttaccgaaat tgaaagacgc tttggacaag gctggctatg ataaggtatt gaaagaaatg
    17461 caaaaacaat atgatgagtt ccgacaagaa taataatgaa taaagacaaa ggggttgtga
    17521 caaaagacgt gtcacaacct cttgttccga ataaacggtg ttcaaaagcc agtttctcgg
    17581 tattaagcta aatatatgta aaaatgtgga aagccatatt cacaaaattt ttcttactac
    17641 tcgaaactaa gcggctattt cacaaactct tggtaaacgg tgttcaatca gctaaccctc
    17701 ggtattcgct cacaaaacga caaaatatga ggaactattt ttccgtttgt ttctctaata
    17761 atcaggtcaa ggtgctgatt gcccaacctt tttttgtata atgagaaagt ataaacttaa
    17821 tgtttggagg agaagatatg ttagggaagg ttttggaaga gttatttatc cggatctggg
    17881 tcattataaa actaaccttg tatttctgga tatatacatt tgccggcggg atcatctttg
    17941 gattaggtgc ggcatggaaa acagtcaatg aactatttta tttgtacgga ttcgagtata
    18001 aagaaatcac aataaaaaga ggctggaata tatataaaag aaatttttta agaggcaatc
    18061 tgctatttag tttattttta tctggtactg ctttattgag ttacaattta tatttatctg
    18121 ttcaaatcaa agggctcatt tttttagcta ttgattttat tttacttttt gccttgctct
    18181 gcctatttgc tacttatcaa tacagtatga ttttagatag tgaatatacg atctctgttc
    18241 ctaatctgct aaagctaagt tttatttcgg ttttttccag cttttcgtct tttctaaaaa
    18301 taattattgg tagcggtata attttaggcg tgacttggca attcaaagga ttgatcctgt
    18361 ttggtgtcat tggtcttttg acggtttgga atggtacgat gacaacacat tggcgtgaag
    18421 aattagataa acagttggaa agttatgaat aagtttcgga ttctattttc aaaaggaacc
    18481 ttactaaatc gtctgttgcg gaaatacgcg gttatcatga tcggtgtaac gatgactgct
    18541 accgttattt ttagtatcca tacttgggaa caaaatcaaa aacaggcaga aaacatgaca
    18601 agtgatgccg ttcaaagcac aagcagaatg ctgaatgata aaacaacctt gagtaggatc
    18661 atcaaaaacc agcttgtagg gaattccgaa aagatcgaaa atgtgacgac ctacttaaca
    18721 aaacccatag atcagtatct gatgtatgta tacgaacagc aaaacagtac cgatgaatta
    18781 gtttctttcc caaatcaaat caaagatctc tacgcaaatt atgaagaact aagtgcgata
    18841 tatatcgtgt tgaatcaact accggagtat tatgaatctt ctagagaaaa caaaggtggg
    18901 gaaataaaaa aggggacacc aaaattacta gacgcctttt atatcaagtt gcctatcaca
    18961 caaggaggag ctgagtcagg aagtattttt attggttttc aaaagaaaga actagatatg
    19021 attttagaaa atctaacgtc attcaatgga ctctctttat acatgatttc tggaacgaca
    19081 aatcgactat acacattcca tgataataaa atagctaaag aaacgtttga aagacaagaa
    19141 atgatcatca cagagagtct gaagaaaaat tcacaattgc cgattgacga tctggaaaaa
    19201 aacaattttg ttcaacacca agaactgtct gatgattatc gtatattagc tgtactagat
    19261 aaaaaaagtg taaaaatcga aacgggaaaa aatcttgccc cgctgatcat tggtatcatt
    19321 attttagaca atatactgct tatctttctt tataaaacat tcaaacgata ttcacagcaa
    19381 gtatctatcg tcatgcaggc catggatcaa acagcagcag ggaatttaga gacgcggatc
    19441 gacataacaa acacggaata tgaactgaag gaactttcaa tcggtatcaa cgaaatgttg
    19501 gatagcatca atcagtttgt agaagacatc tacaaattag aaatcaaaca gcaagacgca
    19561 catatgcggg cgcttcaagc acagatcaat ccacactttt tatataatac attagaatat
    19621 atccgaatgt atgcaatcag cgaaggaagt gaagaattag cagatgttgt gtatgcgttt
    19681 tccgctttgc ttcgaaacaa tacgaatcaa gagaaaacga tcactttaaa agaagaactg
    19741 gacttttgcg aaaaatatgt ctatttgtat caaatgcgtt atcctaatcg agtagcctat
    19801 cattttatga tcgatcctga tttggaaaag atcgaagtac ctaaatttgt gatccagcca
    19861 ttagttgaaa attattttaa acacggtatt gattttactc gattcgataa tgcgctaagt
    19921 gtcaaagtct tgcaggaagg caaacgggta aggatcatca tcaaagataa tggaaaaggg
    19981 atgactgaaa aacggctaaa acagattgaa gaaaaactgt ctcatcctaa agtggaact
//
LOCUS       DACDIH010000046        19558 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDIH010000046 DACDIH010000000
VERSION     DACDIH010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.046"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(34..234)
                     /locus_tag="IV281_001797"
     CDS             complement(34..234)
                     /locus_tag="IV281_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8780787.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(539..1771)
                     /locus_tag="IV281_001798"
     CDS             complement(539..1771)
                     /locus_tag="IV281_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8780788.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2025..2594
                     /locus_tag="IV281_001799"
     CDS             2025..2594
                     /locus_tag="IV281_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8780789.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2772..3212)
                     /locus_tag="IV281_001800"
     CDS             complement(2772..3212)
                     /locus_tag="IV281_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP8780790.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3370..4134
                     /gene="map"
                     /locus_tag="IV281_001801"
     CDS             3370..4134
                     /gene="map"
                     /locus_tag="IV281_001801"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP8780791.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4166..5089
                     /locus_tag="IV281_001802"
     CDS             4166..5089
                     /locus_tag="IV281_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP8780792.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5165..6304
                     /locus_tag="IV281_001803"
     CDS             5165..6304
                     /locus_tag="IV281_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP8780793.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6297..7097
                     /locus_tag="IV281_001804"
     CDS             6297..7097
                     /locus_tag="IV281_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8780794.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7097..7924
                     /locus_tag="IV281_001805"
     CDS             7097..7924
                     /locus_tag="IV281_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8780795.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPK
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7902..8636
                     /locus_tag="IV281_001806"
     CDS             7902..8636
                     /locus_tag="IV281_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8780796.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8736..9602
                     /gene="rfbA"
                     /locus_tag="IV281_001807"
     CDS             8736..9602
                     /gene="rfbA"
                     /locus_tag="IV281_001807"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP8780797.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9616..10188
                     /gene="rfbC"
                     /locus_tag="IV281_001808"
     CDS             9616..10188
                     /gene="rfbC"
                     /locus_tag="IV281_001808"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP8780798.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10210..11238
                     /gene="rfbB"
                     /locus_tag="IV281_001809"
     CDS             10210..11238
                     /gene="rfbB"
                     /locus_tag="IV281_001809"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP8780799.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11336..12187
                     /gene="rfbD"
                     /locus_tag="IV281_001810"
     CDS             11336..12187
                     /gene="rfbD"
                     /locus_tag="IV281_001810"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP8780800.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12222..14255
                     /locus_tag="IV281_001811"
     CDS             12222..14255
                     /locus_tag="IV281_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780801.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14299..15579
                     /locus_tag="IV281_001812"
     CDS             14299..15579
                     /locus_tag="IV281_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP8780802.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYRWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15789..16595
                     /locus_tag="IV281_001813"
     CDS             15789..16595
                     /locus_tag="IV281_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8780803.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16607..17827
                     /locus_tag="IV281_001814"
     CDS             16607..17827
                     /locus_tag="IV281_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8780804.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17817..19403
                     /locus_tag="IV281_001815"
     CDS             17817..19403
                     /locus_tag="IV281_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8780805.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
BASE COUNT         6090 a         3316 c         4073 g         6079 t
ORIGIN      
        1 aaataacccc tgtatccttt gcggtacagg gggttatttt gtataagtag acattgtagt
       61 ttctaaactg ctatgtccta aacgtttcga tacgctaagt atatttactc cttgataaag
      121 taaaatagat gcatgcgtat gtcttagtcc atcaataaac agtcgaagag aaaataggga
      181 aaaattgatt actaaagaaa aacttactag gtcgacggtg tagttgattt tcatacttca
      241 atagattcga attaaacgat ttcgattggc ttataccagt cctatccaac gaaagatata
      301 tcgttcttaa ctctagcggt ataacttgaa gctttacgta aagccaatgg gaatgaactg
      361 gagatgactt tttattattt ttagtaatag ctgatgggtt aatacttaca actttccttt
      421 tcaaagtacc atataaaaaa acggtggggt tgtttagcga aataaaggat ctaagccaat
      481 agcttagatc ctttattcta tgctttatat tattttttgc atgtgtgcga aagtatactc
      541 aagcccaatc gccatttcgg aaaatcggta ctgtagagcc atcttcacgg attccgtcga
      601 tatccatctt gtcagatcct accatgaaat ctacgtgggt ttgacttcta ttcaatccag
      661 cttctgctaa ttcttcatcg gacatttctg taccgccttt cacactgaag gcataagcag
      721 aacctagtgc taagtgattc gatgcgtttt catcaaataa tgtattgaaa aagatgatgc
      781 cagattgaga aataggtgaa gggtcaggta caagtgcgac ttctcctaaa cgacgcgcac
      841 cttcatctgt atcaagcagt tttgctaaga catcttctcc ttgttcggca gaaaaatcaa
      901 caacttttcc atctttaaaa gtaaatttca tacccgagat gatggtgcca gcataactca
      961 gtggctttgt acttgaaatg taaccatcta ctcgatgact gtcaggtgct gtgaacactt
     1021 cttccgtcgg catatttgcc atgaattttt ctccacgagc attatagctt ccagcgcctt
     1081 cccaaagatg gtttttaggc aatccgataa tgatatctgt accgggagca gtatagtgaa
     1141 gggcagaaaa ttgttctcga ttcaattctt ctgctttttt tgctaatttt tcatcatgct
     1201 tcttccatgc taagacaggg tcttcttcat agacacgagt cgttttgaaa atctgatccc
     1261 ataatgcctc aacttgtttt tcttctggca gttctgggaa gacttttgct gcccattgtt
     1321 taccagccgc agccacgaca gtccaactga ctttatttgc ttgagttgcc ttgcgtaaat
     1381 tcattagtgc tttgccattc gctgattgat aagacgctac tcgatggcta tctacaccag
     1441 caaatgcatc gggatcggca gaaacaacgc tgatcctgct tgctcctttt tcaagccatt
     1501 catccgcttg atcgatttta gattgtggta cattctcaat acgatccgtt gcagcatgta
     1561 aaagaaattc tctttggatc tggtcgtcag tccattgaac gataacctca gcggctccta
     1621 gtttataagc ttcttgtgtg atcaatcgag caaggggtgc ctgttccacg ctgatctgta
     1681 aaacaactgt gtgtcctttt tctgtggcta cgccagtttc tgcaattaag cgtgcatatt
     1741 tttttagaag ttcattaaaa tcagataaca taataaaaat tcctcctcac cattttttct
     1801 ggtaactatt agcatgatac catttaatta tgattcaaac aatgaggctt taaataaaaa
     1861 aaggaggtta tttttttaag gttaagtttc atgatatatg ttactacttg ctatttcagc
     1921 ttgtgaagaa aatcaaaaac taattttgca tatcaaaaaa acatgttaca atgaaattaa
     1981 tcaatggtgg aagcgttata aaaaaattaa ggagattaaa aaatatggca agaaaaaaga
     2041 cgattactag agaacagatt ttggcagctg cttatgaagt agctgccacc gatggattta
     2101 cacgatttac tgcgagaaac atcgcgaaca aaatgaattg ttccacgcaa ccgatttatc
     2161 ttgagtttaa aaatatggat gacttgagac aagctctttt tgagcagatc tatgatcatt
     2221 tacaatatga agtgttcact gttgagcata cagggaatac gattatcgat cttgctttta
     2281 attatatcca ttttgcaaga agagagaaga agttgtatcg ttcactgtac ttagaagaat
     2341 atggtggagg aaaagagatg cagaatttct cttatactta ctttgtgaat acagtgaaac
     2401 gcgatccagc ttatgtgaat ctttcagaag agcagatcga atctttgtat aatgggacat
     2461 ggatcatagc tactgggatc gcagctttga tgtcttcaag catcatccat ccaactgatg
     2521 aacagattga aaagatgatc caagacagta tagatgctat cttgaaattc aaagaaccaa
     2581 tcgaaatcga ttgaatatac tcaaaagttc cacaaatcag taggtaacaa gagatgtgtc
     2641 aaaataagga aaagttcgtt tttcttacag cgtatgcatt tttaactccc gaagaactct
     2701 tccatgaatg caatgaataa aagagccata cagattgaat aaatctggat ggctctttta
     2761 ttgcaagtat attaaatttt tgcgactagt tctttagcga aagcttctaa attttcgata
     2821 tcttcttctt cagcattcag atccacttta acattttcgg ctccttttga tgcgcctgtt
     2881 tttgcaaaag cagcatcgaa atcatccacc gatttgcaga attcatcgta aaaagtatca
     2941 ccagatccgc agacaccgta aatttttccc aatagatcca attcttgcaa gtcttcataa
     3001 aagtctacga tctcatcagg cagatcgcca tcatcgtatg tataagtagc tactacgcaa
     3061 atatctgcct cctcgaattc atctgcatct acttgggtac attcgttgat ttcgacttcg
     3121 atatccagat tttcaaatgc ttctgcaaca atatcggcga tttcttcagt gtttcctgtc
     3181 atgctagcat atacgatttt tgctaaagcc atgatgtgcc tcctcgttta taattattta
     3241 acaattatga attataccaa aacatcattg aaaagaaaat aaaacctaaa gacacctggg
     3301 gaatgtcatt tggctaaatg tatgccgcta tgttaaaatg agagggattt gtaaaaagga
     3361 gacgaaaata tgattacttt aaaatcagca cgtgaaattg aaatgatggc agaatctgga
     3421 gctttattag cagatgtaca taaaaatctg cgtgatttta taaaaccagg aatcacaagt
     3481 tgggatattg aggtatttgt cagaaactat atcgagagtc atggcggaat tgctgcgcaa
     3541 attggctttg aagggtatga atatgcgact tgtatcagta taaatgatga aatttgtcat
     3601 ggattccctc gtaaaaagcc tttgaaaaac ggtgatttgg taaaagtcga tatgtgtatc
     3661 gatttgaaag gcggcatttc tgattcttgt tgggcctatg tagttggaga atctacacct
     3721 gagatcgacc gtttgatgga agtaacgaag aaagctttgt acatcggtat tgagcaagca
     3781 caagtaggga atcggattgg tgacatcggt catgctatcc aaacttatgt agaaggcgaa
     3841 aatctagcga tcgttcgtga ttttattggt catggcgttg gtcctacgat tcatgaagaa
     3901 ccagcagttc ctcattacgg agaagctgga aaagggttgc ggttgaaaga aggcatggtt
     3961 atcacgatcg aacctatggt taataccgga acttggaaaa tgaaaatgga tcctaacggc
     4021 tggactgcgt atacaagaga cggcggactt tcttgccaat atgaacatac attggctatt
     4081 actaaagatg gtccaagaat tttgacatca caaggagaag aaggcactta ttgatctatc
     4141 cagtcaatag gtacgggaga ggtacatgaa agtttggaac aaaataaaac aaaatgaaaa
     4201 tctgatgcgc tttattgaga cgactcaaaa gcgcatcact gattcagaaa tgagtacgac
     4261 ctctgtcgtc gttgcttatt atttactctt gtctttattt ccattgatta ttgcttttgg
     4321 taatatttta ccgtttctcc acatcgatca agagactgta ctgacctatt tacgagaagt
     4381 gattcctgag accgtttacc agtttatcgg tcctgcaatc aaagaccttt tgacacaaag
     4441 ttcaggaggc ttgttatccg tttctgcatt agcggcttta tggtctgcaa gtcaaagtat
     4501 caatgctttg caaattgcga tgaataaagc gtatggcgta gaaaatagaa aaaatttttt
     4561 gatcgtccgt ttcttctcac taattgtcat tgtcttgttc atgattgcta ttagtggggt
     4621 aactatcgtt cttgggttgg gacagctgat tttggatgct attcagccaa tcgtacaaat
     4681 acccgtgaac tttatcgatc agttccaaac tttgaagtgg ccaatcacat tggtagcttt
     4741 atttgtcatc atgttcttga tttatttaat tgtccctaat gctcagttga agttgaagac
     4801 gatcattccg ggcacgatct tcgctactgt cggctggatg ctgttatcac aagtgtttag
     4861 tatctatgct aggtattttg caacgaggat cagcggttat cagatcatag gaagttttat
     4921 tgtactcatg ctttggctta attttgctgc tacgatcatc attttgggcg gaatcatcaa
     4981 tgcagttgtt caagaatacg tgacgggtca tcaaatcaaa aaacgtcgag atccaagccg
     5041 aaaatggttc aataaattga aaactaaatt ttctcataaa aaaaagtaga aacaagagtt
     5101 cttattgtaa atttatatca gtaatttata ataattatga ctcttaatta aagtaggtgc
     5161 ccatatgctt gtttctttta gtatggttat aagatttttt ctgacaattt tattatcact
     5221 gatattaaca cctgtattca aaatcatttc agtacaaaca ggtatggtcg acaaaccaaa
     5281 tgaaagaaga ataaataaag ttccaatgcc ttcagcgggc ggactgccta tctttatttc
     5341 atttgttata tcgactttat tcttattccg taaaattatc cctcaatcgt atattgtacc
     5401 gatcttgatt gcttctttag taatcatagt cacaggagtg ctcgatgata aatatgagct
     5461 gacacctaaa caaaaaagta tagggatctt gattgcttct cttattattt attttgtagc
     5521 agatatacgt attgattcct tcacgcttcc ttttattgga tatatccaac taggctggct
     5581 tagttttccc gtaacgattt tttggatctt cggtatcacg aatgcggtca atctgattga
     5641 cggtttggat gggctggcgg cagggatttc attgatcggt ttaattacga taggaatcat
     5701 tggatatttt ttcctccatg cttctacagt gtatattcca gttgttattt tctgtcttgt
     5761 agcaagtatt atcggttttt ttccttacaa tttttatccg gcaaagattt atttaggcga
     5821 tacaggagct ctattcctag ggtttatgat ggccgtcctt tcactacaag ggctgaagaa
     5881 cgtgactttt gtttcatcga tttctttgct gatcgtcatg ggagtgccgg taacagacac
     5941 gttttttgct atcattagga gaaaagcaaa ccgtgtttct ttttctacag cagataaaaa
     6001 acatcttcat caccgactaa tcagtttagg ctttactcat aaaggtgctg tcctgacgat
     6061 ttatgcgatg gctttgatgt tttctttcac agctatggtc atgaattata ctggaagaat
     6121 cggaacgatc atattgatta ttgccatgtt gtttgctgca attttattat ttgaattgat
     6181 tggtctgatc aatgagaaac atcagtttat tttaaaaaca ttgcgatttt tagggaataa
     6241 agagtaccgt acgcaagtaa aacagaaata tttgaagaaa tggaaccgtc atatagatga
     6301 ataacaaatt aaaccagccg atccttatta gtattgtgac ggcaaacagt gataaaattt
     6361 ttcggacact agataatttt atttcaactg taaaagatga aacagcagta aaaatccgaa
     6421 tttttgacaa caattcactt cccgagtaca aaaaaagact aacagagtat aggaaatatc
     6481 cctacatcga tatctatttt catgatgaaa attccggatt tggttatgga cataatcaca
     6541 atctgatagc tagcgattct cgctatggca ttatttgtaa cccagatatt ttagtggatg
     6601 aagagacagt tacagcattg gtcttgttat tgaaagaaca tccggaatgt gcgatgttag
     6661 cacctaagat tcttaatgaa gatggaacaa ctcagcacct catccgtgag cagctggctg
     6721 tttttgatta cgttttgcgc tttgtgcctt tcaaatgggt caaaaaaaag tttgacaaac
     6781 gactggctgt gtttgaatgc cgaggattgc ctactgatgc taatagttat gtacggatga
     6841 tctcaggtag ttttatggta gctgatattg ataaatttaa agagataaag gggtttgatg
     6901 agcgtttctt catgtacttt gaagacaacg acttgtgttt gagttttgaa aaagcaggtt
     6961 acaagttatt gtacacaccc ttttatgcag ttatccattt ttacgggaag ggtgcgcatc
     7021 gaaatgcaaa actgtttcgc gtgtttttga cttctatgat gaaatttttc aataagtggg
     7081 ggtggcgttt tttctgatga aaccagaaat attagtagtc gttgtgcttt atcagcaact
     7141 attcagtcaa tctccagctt atggaccatt aagcaaagca cttagcgaaa agcaaattca
     7201 gcttattatt tacgataata gtcctaaaag gcagcaaaat gagctgtttg aaaaagaaaa
     7261 tatcgtttat catcatgatc cgcaaaatcc tggtttagcg gtggcttata attttgcgtt
     7321 gtctcaagca gatgaaggaa cacgttgtct cgtcactctg gatcaggata cgaggctagt
     7381 cgacgattat tttgaactat tgcgtaaagt aacctttaca gatgaatgtg ttgctgcagt
     7441 tccgatgatt ttttctggga gccgacaagt ttctccccta tattcagatc gttatatcaa
     7501 tcgtacagct caagcagtag aagttgaagg cccaacagct gaacgaatca tggcaatcaa
     7561 ttcaggtgtt gcctggtctg tcaaatttct aaaaaagatt ggcgggttta atcctgcttt
     7621 ttctttagat tttttggatc attggctgtt ttggaaagtg gatcagttgg aaaaaactgt
     7681 ggagatcctt cctgtacgaa tggaacatga tctttcggtt ttagactatg aaaaagtttc
     7741 aagtaatcga taccgttcta ttttacaagc agaaaatcga ttttatcatg aatatgatca
     7801 aaggcattta tccagtcacc gacgacaatt attgttaaga acagctaaac agttcttgac
     7861 cgtcaaaaac cggcatatct ggcggatgac tttgcgctca tatgttaaga attggaaggt
     7921 gtgaattcgt gcgttcggtt tgtatagcta cctttaatgg aggcagatat ttaagagagc
     7981 agttagatag tattttatct cagctttcag cagaagatga agtagtcatt tctgatgatg
     8041 ggtcagtaga tgatacatgg gatatattaa ctgaatacgc agaaaaagat aagcgaatcc
     8101 agcttttttc ggggcctcgt caaggattga tcgctaattt tggttatgcg atccagcaga
     8161 cgaaaggaga tctaatcttt ttggcagacc aagatgatgt ttggttggag acaaaagtga
     8221 aaaagatcac tgactatttt gagcagaatc cggatcagct agtagtagtt tctgatttaa
     8281 tcattgtgga cagcgagctg caagagatcc atgattcgta tttttcgttt cgaaattcca
     8341 aagaaggatg gcttaataat gtgataagaa gtcactatat tggtgctggt atggctttcc
     8401 ggtcttcttt aaaacccgat attttgccga tcccgtcaga agtgccgatg catgatatgt
     8461 ggattggttt gctgggagga aagaagactg ggttcctaag agaaccgtta actctgtacc
     8521 gaagacatga tttaaacgcg agtgagattg aaacacattc taaattacgg caaaaaattt
     8581 cttggcgtgt tcatctgatt acggcgttga tcaaacgaaa atggtttaaa cgttgataaa
     8641 agatgtttaa atccttaata tttccatgac aaatataaca attgatgaga taataacaaa
     8701 gtgaaatttg aaatcaaaaa ggagagcaga tatccatgaa gggaatcatt cttgctgggg
     8761 gcagcggaac acgtctgtac cctttaacaa aggcaacatc gaaacaattg atgccgattt
     8821 atgacaaacc aatgatttat tatccaatgt ctactctgat gttggcggga atcaaagaaa
     8881 ttctgattat ttcaacgcca caagatacac cacgtttcaa ggaattattt ggaaacggaa
     8941 atgatttagg actgcagatc gaatatgcag tacaagaaag tccagatgga ttggcacaag
     9001 cgtttatcat tggagaagaa tttattggag atgatagtgt ctgcttggta ttaggggaca
     9061 atatttacta tggtggcggt ctgtctaaaa tgttgcaacg agcagcttca aaagatacag
     9121 gcgcgacagt atttggctat catgtcaatg atccagaacg ctttggtgta gtagaatttg
     9181 atgagaatat gcaagctttg tcgattgaag aaaagcctgc tcagccaaaa tcaaattacg
     9241 ctgtaactgg cctttatttt tatgacaatg aggtaatttc tatcgctaaa ggtatcaaac
     9301 cttcagaacg tggagaacta gagattacgg atgtcaataa agcatatttg gataaaggaa
     9361 aactatccgt agaacttatg ggacgcggat ttgcttggct tgatacagga acacatgaat
     9421 cattgcttga agcatctacc tttatcgaaa caattgaaaa acgtcagaac ttaaaagtgg
     9481 cttgtttgga agaaattgct taccgtatgg gctacatcga taaggaacaa ttgctggccc
     9541 ttgcacagcc attgaaaaaa aatcaatacg gtcaatattt attgaatttg gccgctgaat
     9601 aaggagtcct tacagatgaa agtaacagat acaaaattaa cagatgtcaa aatcgtagaa
     9661 atggacgtct ttggcgatca tcgcggtttc ttcacagaaa gttattcaaa agcaaaattt
     9721 gcagaacatg gattgaaata tgactttgtt caagacaacc attcactatc agctgaagct
     9781 ggtgtccttc gggggcttca tttccaaaaa ggagaagctg cacagacaaa attgattcgg
     9841 gtagtcactg gagcagtttt agatgtagta gtggatatcc gcaaaggtag cccgacttat
     9901 ggtcaatggg acggatttat cttatctgaa cataatcatc gacagttgct tgtacctaaa
     9961 ggatatgccc atggattcgt tactttgaca ccaaatgtga acttccttta taagtgtgat
    10021 aattactata atgcagaagc agatgggggc atcgcatttg atgatccaga tttagcgatt
    10081 gattggccaa ttgatccatc aagagcgatt atgtcggaaa aagatcaccg tcatccgaca
    10141 ttaaaagaat ttgaagcaga aaatccattt gtttacggag aaatctaaga attaaggaga
    10201 aattttctta tgaaaaacat cattgttacc ggaggtgccg gattcatcgg ctctaatttc
    10261 gttcattacg tggtaaacaa tcatccagaa gtgcatgtca ctgttttgga taaactgact
    10321 tatgcaggaa acaaagagaa cttggccgga cttcctagcg atcgcgtaga attggtcgta
    10381 ggagatgttg cagatgcaga attagtggac cgtctagtaa aagaagcgga cgcagttgtt
    10441 cattacgcgg cagaatccca taacgataat tcattaaaag atcctttccc atttgtacaa
    10501 acgaatatca ttggtacgta tacactttta gaagcttgcc gcaagtatga tgtacgctat
    10561 catcacgtat ctactgacga ggtttacggc gatcttccat tacgtgaaga cctgccagga
    10621 catggggaag gcgaaggaga aaaattcact gcggaaacac catataatcc ttcaagtcca
    10681 tattcttcta caaaagctgg ttcagatctg ttagtaaaag catgggtgcg ttcttttggc
    10741 ttacgtgcaa ccatttcaaa ctgttcaaac aactatggtc cttatcagca tattgaaaaa
    10801 tttattcctc gccaagtaac gagtatctta agcggtattc gtccaaaatt atatggcgaa
    10861 ggaaagaatg ttcgtgactg gatccatacg aatgatcatt cttcagcagt gtggctgatt
    10921 ttgacgaaag gcagaattgg cgaaacctat ctgatcggtg cagacggtga agaagataat
    10981 aagaccgtga tggaattgat cttagaaatg atgggccaac caaaagacgc atacgaccat
    11041 gtaaacgacc gcgcagggca cgatttgcgt tatgcaatcg attcgacaaa attgcgagaa
    11101 gagctaggtt ggcagcctga gtttacgaac ttccgtgatg ggttggctga cacgatcaaa
    11161 tggtacgaag aacatgaaga ctggtggaaa aaagaaaaag aggccgttga agcagcatat
    11221 gccaaaaatg gacaataata cagaggaaat ttcactgtat taatggaaaa acacatgtga
    11281 ggcaggcgct cgcctgtctc ttttttaaaa taaaaatcaa ggagcggata accatatgtt
    11341 tttattaact ggaggaaatg gacaattagg tacagaattg cgccatttat tagatgaaaa
    11401 aaatctgcca tatgtttcaa cagatgcaaa agacttggat atcacagatg ctgagaaaac
    11461 aatggcttat attacagaat taaaacccga tgttattttc cattgcgcag cttacacagc
    11521 agtagataaa gcagaagaag aagcaaaaga attagatgag aaaatcaatg tcgatggcac
    11581 aagaaatgta gcacttgcag caaaagctgt gaatgcacaa ttcgtctata tcagtacaga
    11641 ctatgttttc gatggaaaaa acaaaaatgt agaatacgaa gtagacgacc aaacgaatcc
    11701 actaaatgaa tatggtcgta cgaaattatt aggcgaaaaa gcagtagaag agattttaga
    11761 agactattat atcatacgaa cttcttgggt tttcgggatc tatggacaca atttcgtctt
    11821 cacgatgcaa cgcctagcgg aaacccgcga caaactgact gtagtaaatg accagtttgg
    11881 gcgaccaact tggacacgta ctttggcaga atttatggta tatgtcatcg aagaaaaagc
    11941 accatttggt atttatcatt tgtcgaacga aaatagctgt tcttggtatg agttcgcaaa
    12001 agaaatattg aaagatacag atgtagaagt tgcacctgtt acgtcagaag aatttccgca
    12061 aaaagcaaca cgtccgcagt attcagtcat gagcctgaaa aagacagaag cgttaggttt
    12121 tgtcatacca acttggcaag aagcacttgc gcaaatgctg gaagctgcca aaaaaatgaa
    12181 taagtaattt cctttttatc aaatagaatt tggagcatga tatggaaaaa caaacaagaa
    12241 gtgcacgacg aaaagaaaag ccaaaagcga atcaggcaga ttcgaatttg gtgcttgacc
    12301 tgtttctaaa actccgctat cttattggcg ttttagtcat cgtggtcatc gttactttta
    12361 atttaaacgg aagttcactt ggtgtttggg ataaatatgt gtcgcaaaga gacgatggga
    12421 aaaaaactga tgtgattttt ggacagaatc gggaagtgcg gtcagacgaa tggctggtgc
    12481 agacaccttt ttacctctca caagctgaag aggacttttc gttggtgaac gaggattatt
    12541 cattaaacgg acaaaacatg atcattgcgt ataattctcc tgtaaaggat attacggtta
    12601 tcggaaaacc attcaactgg ggattctttt tcctcggaag agatcgagga ctttcctttt
    12661 attgggcgat gaaattagtc gtcatggtct tgcttggatt cgaagtactg atgatattaa
    12721 cgaagaggaa aaaagctcta tcgctaatcg gtgcatttgc gctgacattt tctccagcag
    12781 tccagtggtg gttcatgcaa catgtgggcg atttgatttt ctttactttg ggactgatga
    12841 ttgcttttta tcattatttc tacaatcatg ataaaaaatg gctacgaacg ttgatgatgc
    12901 ttttggtggt tattttcggt attgggttta ttttagtgat ttacccagcc catcaagtga
    12961 tgctggcgta cttgctgatt ttttattttg ttggcctgct tatttactat agcaagaaag
    13021 tttcatggga tctgttggat gtagtgttga ttggaggggc agttttattt attggcggag
    13081 tcatggttca tttctggcta acgtcaaaag acgcattgtt agcttctttg aataccttat
    13141 atccaggaaa acgggtaagt actggaggaa agtggacgat tggcgaattt ttttctttcc
    13201 ttacaaattg gaaaattcct tttgaagata tcaactattc gaataactct gaagtggccc
    13261 tgttctatca cttttttcca actgtattcc tagcaagtcc atttgtgtta tttggcaaaa
    13321 aagatagcga acaaaagtta ctgggacgta ttttgatgat tttttgtctg ttttctatct
    13381 tttggattac cgttgggttg ccaaaatggc tagcagaaat cactctgctg tcttatgttc
    13441 ctccattaag agccatcctt acttttagtt ttgcggcttg tttattatcg atctggttta
    13501 tcgcttatat ttggagagaa gatgttattc ctatttggta taaattcatt ttgttggcag
    13561 taaatgcatg gtcttatttc tatgcgatta cgcattcgaa aatggataat tatttttcgg
    13621 attttgaaac catcgcagtt gctgtgttgg gaacagtgat tttagctttt gcattatttg
    13681 gctggagacg cttgttttac ctcgcttttg tcgccttagt tatcgtatct ggctttttcg
    13741 tcaatccagt cgtagaaggt acaggagcga tttttgaaaa aacactggca aaagaaatcc
    13801 aaaaaatcga tcaacaagat cctggtaaag tttggttgtc tgaagatgaa ttatataact
    13861 tcaccccaac attgggagtg aaatccttca atacagtacg cttttatcca gatatagctg
    13921 catggaagaa aatcgatccg aaagaagaat acgagaagta ttataatcgg tatgctcata
    13981 caagagcttt tatcgctaag gaagatacaa agttcatttt agatcgtcca gatatgtttt
    14041 cggtcacgtt gaattttgaa gacgcagaaa aattagggat cgactatgtg atcagtaaac
    14101 gaccattaac tgattacaac caattatatg aagcgcagtt cacgcagcta tatggaccgg
    14161 ataaagacgg ttatatgatt tttaaagtag aatatccgga ttatccagat gtaccacaaa
    14221 cacaaactca ggaaaatcag ctagtcgtac agtaataggc tagctgagaa taatcctgaa
    14281 atattagtag aaaggagagt gaagataatt caagcaatcg tacaaaaatt tgtcaacaaa
    14341 atgagtgtcg gtttcttgct tttgttgctg acgctagtct tttttgtttg gacagcaggg
    14401 gtaggttttc cagcaacttt gtttatatat aacaatagtt cattacttat cgcttttagt
    14461 gtggctattt tgctcatttt aaatgtttat aaagcaaaag gcgtcgattt tctttatcta
    14521 ggtgttgcgc ttgccctttt cattttcttt tcttactttc gggaattaag aaatgagtct
    14581 ttatttggag acttactgtt tcctttgatt attttatttt gtttagtaat taaatggatc
    14641 gagtggacaa aatgggatcg cggtatttat ctgattgtct ttacggtctt cctcggaatc
    14701 acgttatatc gtgtatttac agagatccaa gtaccagaag gacaaagcat ctgggctcca
    14761 ggcaattcgc tttctgatat ttggatcaat acgaatacaa tcggaagttc tttgatgact
    14821 cttgctttat tgatcagtgg atttgccagt tcttttgaac gatggtatgt tcgaatcatc
    14881 ggtgggatag cagtcgttgc agccttttta ggtatttggg tctgccaatc tagaggtgca
    14941 ttagtcgcgt tgattgtttt tgcgatattg gatcttttac ctaaatcgtt gatgcgtgtc
    15001 attcgtgcgc catttattgc ctacacggtt acgattcttt tagctttgcc gatttcttat
    15061 ctggcggctg tctctgaaaa agtcaatttg tttaccggtc gagaagatat ttggcataaa
    15121 ttctatcaga cgctaggaga aaaatctgaa caagttttac taggaatgaa aacatttatc
    15181 ttccagcgag gaaaccaatt tttaggaaac cacaatagtt ataattctat tttgaatatc
    15241 tatggattga taggatttgg gattgctgct ttgctgctta tcctttttat tggtcgctta
    15301 acgttgaaag cagatttatc caacgggcag atgactttta tctgggcctt ttttgcggtg
    15361 atgatgcagt catttatgga ggatacattg acttcatacc gatgggttcc gattatttat
    15421 atcctgttgg ctatggccgt tcatcgatat gatgaaccag aagttacgga ggaattctat
    15481 gttatggaag aggcatatga cgatatgact tatgatgata catcttatga ggaacaacca
    15541 gatagcgctc attcttccag gatccaacgc tatcattgat aggcacaaaa tacgagagag
    15601 gctgtgacaa ccgtcgcagc cttttttggt ttattgcaat tgtctagcat cttctgtaaa
    15661 atagaaacgt tagagaaaat cttgtatcat agaaaattaa gaaagaaata agaacgcagc
    15721 tcggtaccaa aaatatggta aaatggcact tgactgtaat tcactgaaaa tcgcttggag
    15781 gaacacaaat gtttaaagat ttgtatttat ttatgaagga catttatcag aacagaaaat
    15841 tactgctaca gttttcgatc aatgacttca aatcaagata tgcaggatcg tttttaggaa
    15901 tcttatgggc gtttatcaat ccgctattta ctgtattgat ttattggctg gtatttggtg
    15961 ttggactgaa agcacaaatg atcgatgcaa aatacccatt tatcgtatat ttagttaccg
    16021 ggatgattcc ctggttcttc ttctcagatg catttgcatc aactacggtt gtttttcggg
    16081 aatatactta tttagtaaag aaagtcgtat tcaatattcg tatcttgccc acgacgaaaa
    16141 tcctatccaa tctgtacacc catttattct tcattctgat tggcatagct gtcgcgctta
    16201 taaatggtat atatccttca gcaatgtctt tacagctgat ttattattta ttttgcatgt
    16261 gtgctttctt gacaggattg acttggctga cggcatcgat ccaaccattt ttacctgata
    16321 tcatgcaatt aattacgatc gtgctgcagt tgatcatgtg gacattaccg atattatgga
    16381 gccctaatca gtttgattca ggtcttgtaa gcatattgaa attaaatccg ctgtactatg
    16441 ttgttcaagg ttatcgtgag tcatttttaa gtgaagcttg gttctgggag catggaacat
    16501 atactttgta tttctgggtt ttcacgttag taatgttcgt aatcggctct gcagtgttcc
    16561 gtagattgaa accacatttt tcagatgtgc tgtaaggaga atagctatga cagaatatgc
    16621 aattagacta aaagatatca caaaatctta taatatgtac gcaaaacctt cggatcgatt
    16681 tcgtgaagcc ctcaatcctt ttaagaaatc ctatcatgat gtattttatg cgttgaaaaa
    16741 cgtgaatgta gaaataaaaa aaggtgagat gatcggtttc gtgggagaaa acggatcggg
    16801 aaaatctaca atgttgaaaa tcattacggg agtactgaca ccaacatccg gcacgatgga
    16861 aatcgaagga aaaatctcag ctttgctaga actaggatca ggattcaatc ctgagtattc
    16921 tggctatgaa aatatctatt taaatggaat ggttctagga ttttcaagag aagaagtcga
    16981 tgaaatggtc gatgatatca ttgaatttgc tgacatcggc gaccatattt atcaacctgt
    17041 aaaaacctat tcaagtggga tgttcgttcg tctagctttt gctgtagcga tcaatgtgaa
    17101 tcctgatatt ttgattgtgg atgaagcatt ggcagtagga gatcttgaat ttcaactaaa
    17161 atgtatggaa aaatttacag aaatcaagaa ctcgggaaag acgattttat tcgtttctca
    17221 tgatattagt tcgattcgcc gtttttgtga tcgttcctat tggttgaaaa atggaaaagt
    17281 tgtcgaatat ggtgatacga tggaagtaac gacgaattac gaaaactttt tgaagaaaaa
    17341 atcagttaaa acagtagatc gaaataaaaa ccaagtagaa cgcttcgcag ctccagatat
    17401 cgtagacgtg acgaaagccg aattgttgga taaagataaa aatccagtgg atatgctgga
    17461 acaaggagaa aaactatacg ttaaagtaac ctatgaagta aaagacgata caatcaaaaa
    17521 tccagtttta ggaattgctc tgcgtacagt cgacaatttt tatgtgtgcg gactcaatac
    17581 tctactagat ggtatcaaga tcccttggaa aaaaggtgag aatatctttt atctggagta
    17641 tgaaaagtta ggtttgttag ctggtgaata ttattttgac gttgcggtct ttgaagaaaa
    17701 tgcaacggtt cctttggtat acaagacaaa atacatgaac ttgtttgtca gcggttctta
    17761 tatcggagaa ggcatcgtgg tcttagacca taaatgggaa gaagggactc atagcaatga
    17821 aatatgattt tgaaatggac ctagatgaac aaagcagtgt gggaaaaatt gccgcacaga
    17881 tcaaaccagg aagtaaagtt ttagagtttg gtcctggaaa cggccgatta acgaaacatt
    17941 tgatcggagc aaaacaatgt gaagtgagca ttgtcgagct agataaggaa ttgttcgatt
    18001 ttgtcagcga gtttgcccaa gatggatttt atggagatat cgaaagtttt gaatgggcaa
    18061 actattatgc tggacaaaca tttgattacg ttttgtttgc cgatgtttta gaacatttgg
    18121 tagatcctgg aaaaacgttg aagaaagtta gagaattttt gaatgaagaa ggagaaattc
    18181 taattacatt ccctaacctt gcgcataatt ctgtcatgat tgatttgttc aataatcaat
    18241 tgccatgggc ttcttatggt ttgctagatg aaacccataa ttctttttat actcatgatg
    18301 gcttccaaaa agtttttgaa aaagcaggat tatacatcaa tattgaagat tatttgtact
    18361 tagctgtagg tgatacagaa ttaaaatcaa cttatgaaga attgccggaa gctgtacgtt
    18421 atgatttcaa aatgcgacca tttggcgaag tctaccaata cttcttctca ttgatgaaac
    18481 atccagtagc acaaagttcc attgcgcagc cgcaaaattc taattatgtg aaagtattag
    18541 aagtaatcca gcaaacgaaa caagatgaaa taattcaaca aataccattc aataatttca
    18601 caggggaaaa tgagacacta agtttccatg tatcagaatt agcagaacga atggtgtttc
    18661 gcttcgcgca gcaacctagc tttatcgaat tcagcgcaga agctgctgga gaaaaactca
    18721 catttatcga ttcaaatgct gtagtcaaaa cagtaaatga ttgttatttg tttgatggta
    18781 aagatttacc agagttcttt ttaacggata ttgcagggaa agaagtgact atccattgtc
    18841 actatcgttt tatcggagaa ttgacaccaa caatgaaaga attgctggaa acaatccgtc
    18901 caatggctga agttacaaag cagctttctg agaaaaatga tgaattagaa agagaaaatc
    18961 attatcttat tgagaataat acaagattag atcagacgtt gaaaacaacc ataaatcgct
    19021 attgcacgtt gttggaaagt gatgaatgga caatcaaaca tagattaggg cgacgtaaga
    19081 aagaaacatc gaaaaaaatc caagaaaaag agctttctct atgtattgac gaaaaaatct
    19141 gggatgcaga aacaaagata ttgaaaatca tcgggtgggg aatcgctaat tcggatcgcc
    19201 aaccattgtc ctataagtta tcagccgacc aatcaccatt ttttgaagca attcctgttt
    19261 tacgagaaga agtcaatcaa gcaaaacagc tagctgaagg agcaaaagca ggatttgaac
    19321 tgcggatcct ttgcgagaag gaacgttcat tcttagttga agcagtggct caaaatggtc
    19381 aatcatggat cattgaaatg taaataatga ttaacttata aagataagga gtaaacgaat
    19441 cgtgcaaaaa aaagaaattg ccgtatttat cgatcaaatc actcgtgaac gggccactgg
    19501 cgatttgctt attgtgggct gggcaattga tgaagtgacc aaagaaatcc ctacgata
//
LOCUS       DACDIH010000047        19215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDIH010000047 DACDIH010000000
VERSION     DACDIH010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.047"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(138..452)
                     /locus_tag="IV281_001816"
     CDS             complement(138..452)
                     /locus_tag="IV281_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780806.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            738..977
                     /locus_tag="IV281_001817"
     CDS             738..977
                     /locus_tag="IV281_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780807.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(1339..1599)
                     /locus_tag="IV281_001818"
     CDS             complement(1339..1599)
                     /locus_tag="IV281_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780808.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1784..2281)
                     /locus_tag="IV281_001819"
     CDS             complement(1784..2281)
                     /locus_tag="IV281_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP8780809.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(2411..3121)
                     /gene="budA"
                     /locus_tag="IV281_001820"
     CDS             complement(2411..3121)
                     /gene="budA"
                     /locus_tag="IV281_001820"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP8780810.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(3134..4798)
                     /gene="alsS"
                     /locus_tag="IV281_001821"
     CDS             complement(3134..4798)
                     /gene="alsS"
                     /locus_tag="IV281_001821"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP8780811.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(5003..5665)
                     /locus_tag="IV281_001822"
     CDS             complement(5003..5665)
                     /locus_tag="IV281_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8780812.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5675..6490)
                     /locus_tag="IV281_001823"
     CDS             complement(5675..6490)
                     /locus_tag="IV281_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8780813.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6752..7195)
                     /locus_tag="IV281_001824"
     CDS             complement(6752..7195)
                     /locus_tag="IV281_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP8780814.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(7329..7667)
                     /locus_tag="IV281_001825"
     CDS             complement(7329..7667)
                     /locus_tag="IV281_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8780815.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7655..8032)
                     /locus_tag="IV281_001826"
     CDS             complement(7655..8032)
                     /locus_tag="IV281_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8780816.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            8256..9449
                     /locus_tag="IV281_001827"
     CDS             8256..9449
                     /locus_tag="IV281_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8780817.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9615..10037)
                     /locus_tag="IV281_001828"
     CDS             complement(9615..10037)
                     /locus_tag="IV281_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8780818.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            10627..11082
                     /locus_tag="IV281_001829"
     CDS             10627..11082
                     /locus_tag="IV281_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780819.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            complement(11244..12416)
                     /locus_tag="IV281_001830"
     CDS             complement(11244..12416)
                     /locus_tag="IV281_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8780820.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(12622..14051)
                     /locus_tag="IV281_001831"
                     /pseudo
     CDS             complement(12622..14051)
                     /locus_tag="IV281_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            complement(14048..15550)
                     /locus_tag="IV281_001832"
                     /pseudo
     CDS             complement(14048..15550)
                     /locus_tag="IV281_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345213.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            complement(15547..17874)
                     /locus_tag="IV281_001833"
     CDS             complement(15547..17874)
                     /locus_tag="IV281_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8780821.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(18717..19010)
                     /locus_tag="IV281_001834"
     CDS             complement(18717..19010)
                     /locus_tag="IV281_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP8780822.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         5625 a         3739 c         3280 g         6571 t
ORIGIN      
        1 tctagagaaa ttaattcatc ctaacataaa ataattgttc actcaaaagt ttttctagaa
       61 caattaaaac agcaggcttc acattcgcta tacatttgta agaagtaata attcctgtac
      121 taaaaggcat aattgcttta attatttttt gacttctctt caaacacaag aagtagttgt
      181 gtaataattg cagaaataag gattccaata atagatataa aatttgacat ggaaacgtct
      241 ataacattaa aagaataaag tgttgaagaa agtaaactga ttgaaataac ccctccgtaa
      301 aaaatagtct gtgaatatac ccactttttt tcagaagacg tacttcgttt ggttctaaaa
      361 cctatccctg catttctact cttatcaaaa aaccaaatat ttagaagtaa taatattgct
      421 attattaaga taaaattact gtaaaaatcc aaaagctatc ctcctagcaa ctatttaaat
      481 acccaaaaaa attacctcta gataatatca tacctacgtt ttcacttata aattttttca
      541 taaaaaaagc gtttagaggc ataatcgtta atacagatgg taaccaatgt atctggtttg
      601 aactgggata tcaccctctc catatatgta ttactaagac taatagatac tataaaagac
      661 ttgatagttt tgcattgatt ttgcctaata ctgagcatag gtgtatgcta aacgatacta
      721 taaggagtgt gtacataatg aaaagaattc tgtttatcag tttatcaaat ttattgttac
      781 ttactgcttg tgggacttct gcatcaaaga acataccttc agatacgcaa atttcgaata
      841 gtatcgattc aacaatttct acaaatgaga aaaattctcc ggtagaaact tccgatataa
      901 aagtttctac ctctgcaaaa aacagggtaa agaaactgtc agcacgtctc ctttatttca
      961 aacaaactct gaattagctt cttcatcttc ttcagcaaat aatgatgata gtttcgcgtt
     1021 tactcctaac taaatgcaac ctttacaaat gctttacgat aacaagcctg agctaaatga
     1081 tcctgatatc agttttgcat tttttgaaat gattggatcg gatgatcttt ttaaaactac
     1141 tagtttgtct atccgaaaat aggacagttc tggcagtata ggtttttatc gagtttctcc
     1201 accaggcctc gttacagaga cagattctct aggcgaacct tttgaataaa tcaaaaatca
     1261 tgaacaatca ctgccaacta aataaagtgc cttcaaaagc ttggtcttct cgcttttgaa
     1321 ggcactttat ttaaatcttt atgaaactaa agtatgttca tatttgttgc tatagttata
     1381 tagatattct gttgtttcca actcataaac gtgagtatat tttgccacta cttttccgca
     1441 tttttctaat atttcattgc gatcgacgtc atgatattcg tctacgcaga tcacaacaca
     1501 attttctaat ttactgatta cttctccaat cactttttgt ttcaagccaa taggttggca
     1561 aatatatttt tttcctaaaa tgatagtttc gttattcata tttcatccat ccttaatgtt
     1621 ttttcattat ttaaaatgat aataacatta aaatatgatt atgaaaatca ataacacttg
     1681 tgattttata aaaaaataaa aaaaccgaaa aacagaaatg cccactgtct ttccggctct
     1741 ttttattctt gatttctctt cttttttgtt tcaatagacg attttagtta ttaataaatg
     1801 aaaaagcatt tatattaaaa tttgctaata aaagatctac atatttttct agaaatgctt
     1861 gatcgatttc atcataccct ttcgttactc cgcaatcgat atctaaaaca ccgattaaat
     1921 taccttcttt taccattggc actacgattt ctgattgtgc ggctgaatcg caggaaatgt
     1981 aatttgcatg ggttttgaca tcatcaacga tcaatgtttc ttgtttttca gctgattctc
     2041 cacaaactcc ttttcccatc ttgatacgtg tgcaagaaac tcgcccttgg aacggtccta
     2101 aaatcaattc attaccatcg aataaataat atcctgcaaa gacagtctca ggcaaagtct
     2161 cattcaacaa tgcagatgaa tttgctaaat ttgctaacca gttatcttct gcttctaata
     2221 atccttgttg ctgtaataat aaaatttcgt atgcatcttt tttctgtttt tctgtccaca
     2281 tgataaaaac cgctcctttt tgatctatgg agaagattat agctcagttt ttttgtaaac
     2341 gctaggtttt ctcctcaaaa aaacgagata cgtcaggtta atcaccttga acgcatctcg
     2401 ctgtcttttt ctattctgct tgttggattt ctgctgctaa atcagcataa tcaatcgttt
     2461 cgtgaacaaa agtcggatca tcaactggaa aatgctgtac aagggacgag atgcggctaa
     2521 tctcaacttt tccttcagcc acttcaaaat ccattacatg tccgccaaat gtatgtgtat
     2581 catcgataaa atgcaggtga aatcccgctg cagcaactcc ttggaataat tcaggcgtaa
     2641 agaacccgac taatgttcct tggacattgc tttgttggaa ttctggttga gactctgaaa
     2701 tttttgctag tcttctgtaa ggtttttctt gcttcggcat gatacggatg tgcatatttt
     2761 tgaataatcc agttatcttc actgccaaaa ataggttttc cccggcttct cttactaata
     2821 tgtctttttt caattgttct ccatctgcag tttttgagac agaaaaacta gtatctggcg
     2881 taaaatctgt aattgctgca tagggggtaa gttcacttcc atctaattgg gcaactgagc
     2941 catctgatct tccttgataa gctacgccat ctaaaaagat gacttcacca tcaagcccat
     3001 gcaatgttcc caatcccaga gttccttttt ctaatatatg agcaatcgtt tctgttccat
     3061 ccatcaagcc agccatcaaa gctcctaatg tcccgtgctg gtataagatt ttttcagtca
     3121 tgtcgacact tccttaatag aattgatctg gtaagattgt ttctcccaat ttctcattat
     3181 cacggtaatc aatcggaata tcaataatta cgggtccttc tgtcgcaaat ccttcttcta
     3241 aagctttttc taattcctct acacttgtag ctcggattcc tttagcgcca aaagcttctg
     3301 catatttcac aaaatcaact ggaccaaagt ctacacctga cgcccgctga tatttcattt
     3361 tttcttggaa ctctaccata ttgtagtgtc catcattcca aatcaaatga acgatattca
     3421 attttttacg taccgccgtt tctaaatcct gggcagagaa taaaaatccc ccatcgccgg
     3481 aaactgatac gatttgtgta tttggacgca ccaatgcagc agaaatcgcc catggcaatg
     3541 caacaccaag cgtttgcata ccattactga ataacaaatg acgaggttca taacttctaa
     3601 agtggcgtgc catccaaata taatgactac ctacatctac ggtaacagtc atatcatctg
     3661 ttacttttga ctgaagcgtg ttaataactt ctaaaggatg caagatacct gcttctcctt
     3721 ttgattgaac aatgtcccgc tccgttaatt ctgcttgaag agatgctaaa tattcttttg
     3781 catcttctga cacttgttga ggctctaatg aacctgtcaa taaatcaaga gtggctgaga
     3841 tatcaccgat caattctctt tcaggctgca taaaaggatc gatttcagct ggcgcttcat
     3901 cgatcacaat gatccgcgca tctttttccg cattccagtt tcttgcttca tattcgattg
     3961 gatcatagcc aatagcaata actagatcac ttcgtttcaa aagcatatct cctggttggt
     4021 ttctaaataa acccacacga ccaaagaaat gcgcttctaa ttcacgtgag atcactccag
     4081 ctgcttggaa tgtctctaca acaggcaatg ctgtctttcc aactaattgc ctgatggcta
     4141 acgtttcttt ttcacttgat cctcgcatac ctactaatag taccggtagt tttgcttctc
     4201 taatgcgttc agctaggtaa cggatatcat ctgcagaagc agaacctaat ttaggatcgc
     4261 tcaatggctt gatgacattc ccttggacag gtgcatccac tacatcttgc gggatactga
     4321 taaagctcgc gccttttttt gaactcttag ccatacgata agcattggca atcacttctg
     4381 aaattgtttc tggatcttgg atttctgcac tgtattttgt gattggctgg aacagtgctg
     4441 cattatccat gctttgatgg gtcaacttca atagatcgct tcgttttact tgtccggcaa
     4501 ttgctaaaac tgggtcacct tctgctgtcg ctgttaccaa acctgtagct aagttactag
     4561 caccaggacc acttgttgcg ataactactc ctggctctcc tgtaatacgt ccgattgctt
     4621 gtgccataaa tgcagcattt tgttcgtgac gagtcacgat taattcagga ccttgatcct
     4681 ctaattcatt aaatacacca tcgatttttg cacctggtat accgaataca taatcaacat
     4741 gatgattaat caaactttct actacagatt tcgaaccttg tttcattttt tcgtccattg
     4801 aacatcgtcc tttttatgct ttatttttaa ttattagtta gaaataatca caaataattg
     4861 atacagtttc taaacctgtt tcattcaaaa gtatagcacg ttttatttca tttgtttagc
     4921 catatgtttc aaaaaatgaa aaagaatgac agtttttaca taaaaactgt cattcttaat
     4981 tgaaagtttc aatttataaa gtctacagat ccatagttct catcaattta cgattgttga
     5041 agtaaatcaa accaaatacg atagaaggac ctagtagaat acaccattgg ggctttacga
     5101 aaaagaagag aaacaaactt gtcaatagta aaaaatctag taaactcaca taccattttt
     5161 tcaacgtata gtagaacgct atccgtagaa cttgatttgg tttcgacgct ggatttctca
     5221 cctgaaaata ccagtagttg atgctaaagg aaactgataa acaagcaagt aaaacaaaca
     5281 acggaatcaa ccattttcca taagaaaaac gtataaaaaa gaaacaatca aaaatcatta
     5341 tcacactaac tatccaagca aatagccaat aaaaaaatcc ctttagccaa aattggcgaa
     5401 acgtctttat gaacaaacgt actggtgaaa aacttttttc ttttgctaat tcttctgtca
     5461 tacctatcat cacgatgcaa cctggaccaa taaaaagtaa agaaaaaata aaggaaaaga
     5521 cattccttcc atctatcgac aaaaggaacg cagctgctat atacaagata ttgacacacc
     5581 aaaaacaaag gctacttgcc aatgattgat acaaccaata aaagaattcc atataaacat
     5641 ttttgtcaaa acgctgctct ttcataatca cctcctattc cttcattcct gatgaagcga
     5701 tcccttctac aaaatatttt tgcatcgcta aaaatataaa agcaacagga agaacagaaa
     5761 caactgaggc agccatgatt aatgcgtact ctgcatcaaa tagcccaata aacattttca
     5821 accctaactg gattgtttta ttttccgtag aagacaaata aatgaatggc cccatataat
     5881 cgttccatgt attaacgaat gtgatgatcg tcaaactggc aatcgccggt tttgtaagag
     5941 gtaaaatgat cttgcgatag attccccact ctgataagcc atcgatccgc gcactctcac
     6001 ataaagaatc tgggatacta ctgtagtatt gcttcaacaa gaacacacca aaagcattga
     6061 acgcttgtaa tagcaccaac gaccacaacg tatatgttaa acccactttt ctcatcataa
     6121 tgaactgcgg gatcatataa gactgccaag ggacagcaat cgtgccgata tatgctaaaa
     6181 ataaaatatc gcgcccttta aaatttagtt tagaaaaccc gtaagcagca aaactagaag
     6241 taaacaactg aatcaacgtg atcacaacgc ttaaaaaagc tgtattctta aaaaatgtca
     6301 ctaaaggaat acgagaccaa ataaccagat aattctctaa atgccaagtt ttagggatcc
     6361 attgaatcgg aatcgtgaac acttcattat ttgctttgaa tgaagcagaa accatccata
     6421 aaaaaggaat gatcgtcaat aaagctaata caatcagcag aatcataccc aaccattgaa
     6481 taaagggcat gttttttgaa cgaatttttg ctttttttct ttcatttcga acacctccaa
     6541 agttataatc ttcatttctt tgtaatgatt acaatccaga acaacggtta ttatagctga
     6601 ttcatttccg ttcttcttat gtttgtaagc gcttatttaa caagttatgt aaatctcttt
     6661 attttttttg atttaaaaat gaaaaaatac aaaagcaaga ctgtcatacg tctcgctttt
     6721 gtattgaaat ttcttcgatt agagatactt attactgttc aaaagcttct ttgaaacggt
     6781 gaatcaattc attgactaca ggtgataaaa tccgattctt tttccagact agcatgcagt
     6841 tcgtttcaat cattgggtca agcggaacga atgtcactcg ctttgaaaga tgacttacag
     6901 caccttctat gactaaagca gcacctacct gattctcaat tagcgcaaat acattgaaaa
     6961 tcaaattgta cgttcctgtg atcagcaact gctccttttt tatgcctaac cagtcagcaa
     7021 acattcgctg tatttctgga cgctttgaga tcaaaagagg tacattcgca aagtcttctt
     7081 ttgttatcac gtcttttttt gctaaaacag aattttctaa aatgagcaat ccccattttt
     7141 cctttcttgg taacaaaatc gtttcgtatt tctctgtatc tactggatct agcaaaatcg
     7201 ctaaatctaa caggcttttt tctagtcact ccgtgatatc atcacttgtt ccgctgtgga
     7261 tatgaaattg aacgctggga taatcacgga tcaacttttc tagtaatagt gccaatgtat
     7321 tcgaattatc agcttccaca cagccgatcg taaatcggcc gctgaataat tgtttttttc
     7381 tgttctcgaa ttcttgagtg gtttgattcg ttaaatctaa aatttcttct gctcggcttt
     7441 tttagaaaat aacctgcttc tgtcaagatc attttgcggt tttcgcgtac aaacagttct
     7501 gttcctagtt cctcttctaa ctccttcagt tgtcggctta aggttggttg tgtaagatgt
     7561 agaacttctg cggcttgaga gattgttcct tcttctgcaa ttgtccaaaa atattttagt
     7621 aagcgtaact ccatagcgat gctccttgat ttttttactt gatacattcc attttatttc
     7681 tagataacac acgatcatat ttcttgattt ttccttgtaa acgttccaaa cattcttgca
     7741 tttcagcaat ttttttcgtc aaaagttcac tttcttcaat caaaagctta cgccgctcaa
     7801 atacagttgc ttctccttcg ctagataggc gaatatattc aatcaaagac tcgatcgata
     7861 atccggcatt tcgcatacag atcgtaaagt cgacccattt taggtcttct tctgtataat
     7921 cacggattcc gccttttgtc cgattaactg gtggaatcaa cccgattcgt tcataatagc
     7981 gcaatgtatc ggccgaaatg cccttttctt cactgacttg tttgatattc atctgatcac
     8041 tctttctctt aatttcttgc cactttactt atgtatttta tttattctac aactttaagt
     8101 gtactccaag tcaatactca tttctattaa aactaagctg gattatctat atcggttgtt
     8161 tgtgtattat ttcaaataaa aaagtcttgt ttttttattc ttttttggat atacttttaa
     8221 caatttaggg aaattttagg aaaaggggta taaaaatgac aacgtatcag cgaaagttat
     8281 tattatcgac atcggcggga attgctttag agaatatgga tattatgttt ttagcttttt
     8341 cattatcttc aatgattact tcttttcata ttagtggtac gcaagcgggt ttcatttcaa
     8401 cgatcactaa tttggggatg ctcattggag gtattttttt tggcatcata gctgataaat
     8461 atggacgtgt gaaagtattt tctcaaacgg tccttctgtt ttcgattgct tctttattga
     8521 tgtattttgc ttcaaacatc tacttggttt atctctttcg ctttatcgca ggtgtaggag
     8581 cgggaggaga atacggggcg tgtatgtcct tgatttccga aagtttttca aaaaaacaaa
     8641 ttggacgagc ttcttcggtt gctggtattg gtgcgcaggt cggtgcggct ttggctgcga
     8701 ttttagctgc tgtgatcatt ccaacgtttg gttggaagat gttgtatgtc gtaggtgtac
     8761 tgccggtgtt gatggtcttg ttcattcgtc gcggcttgac tgaaccaaag gaattcaaat
     8821 ctgtacaatc tgccgaaaag aaaaccaaac ttagcagact gtttgaatca aaacatcttg
     8881 cttggcaaac atgtggcctt agtatcatgg taactgtcca aattgccggc tattttgggt
     8941 taatgaattg gctgccttca attatgcaaa atcgattagg gctttcggtt tctgggtctt
     9001 cattgtggat ggtcagtact attttaggca tgtctttggg gatgatgact tttggcataa
     9061 tcatggataa attggggcct aaactagcct tttctttgtt tttaatttgt tcatccttat
     9121 ctgtctatct acttgtgtta gcccatagcc agtggagttt ggtagcagcc gctgtgatag
     9181 tcggctactt tatcaatggc atgtatggtg gatatggcgc agtgattagt agtttgtatc
     9241 caacagaaat ccgcgcgacg gcgaataatt tcattatgaa ccttggacga gctgttggtg
     9301 gtttttcttc tatcgtcatc ggtttcttga tggatcatta tagtttaact gccgtaatct
     9361 tgtttttgag cggtatttac ctagtcagtc tgctggtctt gacaactctt actggtgtta
     9421 cagcattaag ggaaagcttg cagcattgat agttgagtct gtaaatgaca agaaagaggt
     9481 gtggcaaaag cttgcatctg cggtaataag accagccaaa tcaaaaacat gagacactgt
     9541 ttccgtttgg ctggtcgtat tacttgatgc agggcagctt tttcaactgc taatttcctt
     9601 ctatctggcg gctactattc tttgacgtcc gtcatttcaa agtactttac taataactta
     9661 gcataacgaa aaactgtgga tacactcgta tattgagcgt ctgcataatc ttccaaattt
     9721 ttctcaggat gttgatacat gaattcaatc aaatgataat tatatgattt tctgtagaaa
     9781 tgaagataaa taatgtagat tgggaagttc tcctgctttt ccatagaaaa gcgccgatta
     9841 ttctcattct tcaaaatgac cttatcagga tatttttgga acaattcttc taaatctgtt
     9901 agataattca agatagactt acttgatacc ttcaattcgt tttgtagttc ttctattgaa
     9961 caccaacgtt ctgtttcaag cagtaaatga atcattttaa ttttcttttg agaaactttt
    10021 gttaaaaaag tctgcatatt ttctcctcct gtatctactt tactatacta catacaagaa
    10081 aaaaaacgga tacaaatatc atattgtcac accgtcaata gtgcataaac ctgttattat
    10141 ttctcttttt ttcctaagta ttctattgat atgaatcatt taatcgttta ttatataaat
    10201 gtcaataatc taataataaa ataacgtaca tattttatta ttgcatactt aataacagaa
    10261 ctatagcaaa aaatcccgtg tatcaaaacg ggattagatt aagtttcctc aatcactatt
    10321 cataacacaa ttattataac aagtaagtaa acaattgaaa aggacattta ttgaaaacgg
    10381 ttgtttttta cttaaggagg aaatttagtg aaaaaattag ttgcattgtt tttggtcgca
    10441 ggaagtttgt tgagtgggtg tggtgctact acgtctacta ctcaaagcac aagttcagct
    10501 caggcttcca gcagcagtac cacgatctcc gaaagtacgg cagattcatc gaaagaacaa
    10561 gaacaaattg aaaagaaaaa agaagaagag aaacaaaaag cggaaaaagc tgcactagaa
    10621 aaaaaagtga cagaagcgga atctgctttg tcacaagctg aagcaaatcc tacaaaagaa
    10681 aactatgcaa ctgcttcagc agctatccaa gcgctacctg aaaacaaaca agagttgaca
    10741 agccgtcttg ctgcaattga ttccacaatc aaagcaaaag aagcagaaga agaacggaaa
    10801 aagcaagaag aaattgcacg acaacaagca gaacaagcgc aacaagaagc aatcgcacag
    10861 caacaagcag aacaggcgcg acagcaagca gcagctgaac aggcctctca gcaggcacaa
    10921 gccgcagctc ctgagcaaaa taatgaacaa actgtctatg tcacaccgac cggttcaaaa
    10981 taccatacac ataaatgtgg aaatggtacg tatacccctg ccactttaag cgaagctcaa
    11041 gctcgtggtt taaccccttg ctccaaatgt tatggaaact aattcaaaag ttatactata
    11101 ataataaaat tcttctgaaa cataaaaaaa tagaagctgg cgttccagat agtaaaccaa
    11161 aaatagcagt ttacacgtcc atttcgtcag cttctctctt ttttcattta gtctgttccc
    11221 tctgcttttt tggacatctt cattcattct ttctcaatca aatagtacac ctctttttct
    11281 tttcgcagtt tatatcttcc ttttttcaat ttcattgaaa actgtttcga atgtttatgt
    11341 tttacatgga ttttaagcag gagctcctca tgttgcaagc gatagttcct gccttttaaa
    11401 tcagaaaatc tcactagtcc gtattcatca gaagctttca caaatggaac tttttctcta
    11461 gtaatatagt gtttgccgtt gagcgttttt acagaaaaga ccattccttc gattggtcgt
    11521 ccgtttgtgt tctttacata aaaagataaa ggatagtttc gcttagcgat ggccaaagaa
    11581 tctaaataag cgatgagtag ccaaactaag ccgattgatc caagtagtat accgataaac
    11641 caaataaaaa atttccattt tttccattga tcaattcctt ggatcgcttt ttttgcctct
    11701 tttgcttgat aaggaatacg atgccccgtc acaagcagtc gatgtgtatt gaccatataa
    11761 ggagtacagg taagcaaagt cacgagatct ctgccttttt ggatacccaa agaatctact
    11821 tcatcaggta agaccacttg gattttttct acttgatacg ccaatgtctt tccattgatc
    11881 tggatgaaaa actggtctcc ttttttcaac ttaggcaaat ctgtaaataa ttttgcttct
    11941 gggagtccac gatgcccaga aagaacggca tgggtgtttt tcccgccagt cggataagaa
    12001 ctttcttcca ataatgacgt tcctttttgc aaaaatattt ccgttgtctg atcgaatata
    12061 ggtaagctga cattgatttt tgggatttcg atgactccaa gcatatgttc cataaaaaag
    12121 gcattactag gtaaatcttt taatgctttt gcatcaactg cttggttgaa actgcctatc
    12181 cctggtgata cgttttgctc tgccaactgt tggtttttct ttgtcatttt ttcctgctgt
    12241 ttttttattt cctctgaatt cttattcgat gcttgctttt gataatggat gatcatttgc
    12301 tgggcaagaa aatcattcag tgaatcttct acaaaaggat aagaaaagat cccgatccca
    12361 acgactaaaa tgattgccat gaagatgtcc agtattctta ttctgctttt attcatcatg
    12421 tccacctatt ttctaatatg tatatgaaga ggttgtgaaa tcagcgcctt gacctgagta
    12481 ttcgctcaac aaacggcaaa atagttcctc ctattttgcc gtttgttgag cgaataccaa
    12541 gagaccgacc acttgtcaca acctttctcc ccatttgaat cactatcgtt tatttatcgt
    12601 atgatttttg tttccaatag tttaagcttc tgtttcttga tggcgttttt tgaagtagat
    12661 cactgctcct ccaactaaag cagtacctac caagatgaat acgatgatcc ccataccacc
    12721 agttgaaggc aagattcctt taggttggtt atcgatcgtt gtttcatcag aagttgttgg
    12781 atcatcatct actgtaaatt cagtgtacgc agcttcattc ttaggtaatg cgtagccttc
    12841 tggtgctttt gtttcttgaa ggcagtatgc cccttgtttc agcccttcga ttgaaaattt
    12901 cccttctgaa tcagaaatca atttcacttc ttcccagtca gaacctgtga cttcatctga
    12961 ccaagcatat gggtattctc ctgaggcaag atttgctatt tttttcatta atttaacagt
    13021 tggatctttt gctcttagaa tagaaaattc tgcatcggct aatttttctt gcgtgttcac
    13081 atcttgtttg atgaagtgga aatcaccgta gttttggaca ttttctacag taatcgtgtt
    13141 atccgtagtt gtattttccc gattgactac accgtttgga ttcccaagat tggcttcatc
    13201 tgcgccatgg atgataccgg ttgtttgttg caattttccg ttttttacta taaaagtagt
    13261 ttctttttgt ggattggtta attgataacc atctggtgct tttgtttcaa ctaaagtata
    13321 attgccgtct attaatccat tgacactgat ggatccttca gtatcagttg tgaatgtcaa
    13381 cgctgactca gcaggatcat agagagcgac accagtgttt tcatcaaaac cggtcacgaa
    13441 ttgattctct gaatttttga tctggaattg cgcccctgct aacttatcta cccctgaaaa
    13501 gttcccagct ttctggattt tgatattgct ttctttgatg acatttttag catataggtg
    13561 aatcgtgtct aaaggtttgt cagggtctga tgaattcggg ttataaaagg gtaagcttaa
    13621 cacgatattt ggcgacgttt cgacaccggg ctttggtgtt tctacaaaga cgtatacagc
    13681 atctttccca tcagatttac ctggaagagt caggttagct aatccttcac cagaagtcgt
    13741 tacacttgcg aagggtgtag cgtctgctgg aactgctgtg tctttacttg gatagtacgt
    13801 agcagtgatg tcatatacgt tgaattcaac gccgttcaac ggttcgctat tcggaaatgt
    13861 caattccgtt ccgttgttta tttgattttc tggtgctgaa gtgaatttca atttatgcaa
    13921 attaaccgta acattttctg gtgtaggtga tacggcctag aaacctctgc agttcctagg
    13981 ccgagaacga aaggaagcag catgatagca gttcctaaaa acgtccatat ttttgttttg
    14041 atgtttttca ttttatttcc ctcctattgt ttcctttttg ttgtttttta taaaaccaag
    14101 ctgtccctgt tgaaaggact agcaacaacc ctaaaattga gaaacctcca agcatctctt
    14161 cgcttgtttt tggaaagctt cctccatttt tttcatttga tggaccattt gtctgcatct
    14221 gaactggcgt ttttccatta ttcgtcggat ttgcttttgg ttcgttgatg atttccaatg
    14281 gtataggatg aagcggatca taactgcctt tttcaacttc aaagagaatg gttgattgac
    14341 tcaatatata tcctttaggc ggtttaatct ccactagttc gtaacttcca aaagacaatc
    14401 catggacaga aaatgttcca tctttactgg aagtaaaaat cgttgctctt gaatcatctt
    14461 tggctaccca atgatttatc ccgttttctt gcgctaagta ttggtcattt tttttgacta
    14521 gaaattgagc atctgctagt ttttgtttcg tttgccgatc cactttgaca aattgtttgc
    14581 ctcctgtttg gatggcaaga tgtgtttcta ctgtttgatt gtcagtcgtc aaacgtacat
    14641 cgttatcaaa tgtcgttttt tcactgtttt ctttcaatgt catttggtaa cggatcgtta
    14701 attttttccc agcaaacgaa ctaagatctt ttccagaatc tgataagaca aaaccatgtt
    14761 ttgttttttg tgtgtgaaat gtatgaatct tgtctccacc aatcaaaaaa tcgatcgttt
    14821 ctggttctag ccataactga ggatcagctg tatctttgat cgtataggtt cctatctcgt
    14881 tgatgttctc aggaattgtt acggtgatga gataaggaac tttatcccca tctgcaaaat
    14941 ctgtctgctt attctctact gtttttgtta atgtgagtgc agcaggtatt ttttgttcgc
    15001 tctttggata caagtggatg gtcgtcaatt tatgattaga tgaatctgtc acaggaagca
    15061 caacgacaaa tggtgctgat ttttctttta cttaatctga agtctttatt tccgcaaatc
    15121 ggtaaacagc gtcacgacct tgctcatctt tgtccgatgc actaaaagaa gcaatgccct
    15181 cttcctcgtc tactgttttt gtcacatttt ctgccagtat tttttcacta tcactttttt
    15241 gtgctagttt tctttgtgcg tcttccacac tacttccttc tgaccgcagc ttgtaaaatt
    15301 catccgagac atcatatata gcgaaagtaa cgccgttcaa cccttttact ccttgaaagt
    15361 tatccgggat atttattccg tcgttttgac tgatacttgg catcttatcg ctttcgaaca
    15421 ttcgattatg caaagtaaat gtaataggtg attctctagc tgacacagtc ggagaaccta
    15481 taaaaaagac cagtaggata aaaaagagaa cagcaaatat atccacacgt acattttttg
    15541 tttttttcat cgctgttcac ctgctttttt cttacgatgg ataaaataaa agaagccaga
    15601 aaaaataaag atcagtccta aaaagacaaa gggcagtaga cctatcccac ctgtcgaagg
    15661 aagcaagaga gtcggattga tcgtcaactt agcggtatct gttgcgataa tcgtataatt
    15721 attgtttaca cttccttcgc ctaaactaat cgttactatt tgtgatccaa tatctgtccg
    15781 gctgttaact gttagggtat aatccaatga ttcgcctaag acaacaccgg taacgtggag
    15841 atttccaacc gaaatcgagt gactttgggc atcataatta aaggcagcgt catcgacagt
    15901 aatcactgcg ttagctttat agatggtata agttcctgat gtgttggcta tatcagtaaa
    15961 tgacaacttg ggatttgctg ctttgacttt gtctattccg ctattcgtta gtttaattgt
    16021 atagctgcca gcattaacca tattttttgc tgattgtgca tctgctgtgt aatccatact
    16081 gctaagtgcc acttgtactg gagtaagcga actagtgaca ccctctgcta gcttgacatg
    16141 gattgacggg acacctgctt ttccatcata tgtcttactg ccactgagct ctaccgcacc
    16201 gctgatttgc ttaatcgtat agacatcaga tgtctgcacg atagtaaata aatagtttgt
    16261 tctggcagct agtttagcta gtccgctttt tgtcaattgc caagacgtat aggttccaat
    16321 ttcagtaggc gcagttactt caactccctt ttcattcatc gctttgaaat cagttggatt
    16381 ccatgtgatt tgatcaatac tgagatcatt gtttaaaagg gatgttgatt ctgttttatc
    16441 attaaaggca tatttaacga tcggtacaac agaaagacca tcatagtact tactgccatt
    16501 tgcattgatt gatactgttg catttgtctt ataatatagg atgacttcag aaatctgttt
    16561 tccatttgcg tcctttacga ttacatttcc atttgtatct aaactagcta aaacactcat
    16621 tgctgattgg ttctttgttt ctgtcgctga aacagctgta aaggtatatc cgttgatagt
    16681 aggcgtatta gtcgtcaggt cttgtgcagc acttccgtaa gtaagattca ttattttaga
    16741 aggagcaatc atctgtccaa aatagtcttg atacttaata gtgattggtg tttgtttgcc
    16801 tgtataggtc accgtatcga cgttactttc tagggctgtg aaagaaccgt ttgtttgtgc
    16861 aaaagaatag cccgttactg ctgttggagt gataaccact ttgtcaccca catggattcc
    16921 agataatgta gtcccgtacg ttccgatttt tgctgctcca ccattgactg tataagaaaa
    16981 agttcctgtc cgattcgcat tgacctgaac cgttgctgct tgataggttg gaaaatttga
    17041 aacatttccc ttaccatcaa cgatctgccc atttttgatc gagtaccccg tgaatggaat
    17101 atcatttgct aactgtatac cgattgaaaa agtactcata cctcctccgg caggaagccc
    17161 tccccatgtt gagggtgtaa aagaataact tttatccgca ttgggtgtaa ctgaatcgat
    17221 cgtatttcca gcggtagcca cccccatata gatacttttt gggaatgtga tagtagtacc
    17281 atttactgtg gagccaataa acagattatg gatatctgga cggttatcaa taaaagcgat
    17341 tgctttggtt acgccttgta acgcagtttt atccgcactc atcgatccgt taaccgcaac
    17401 aatgactcca tctttggcat atatcactgt atatccatct ggtatggtat gtccgttgaa
    17461 attcgcagtg gccgcaacag tactaaatgt ctggcttcta cctgtttctg aataagcttt
    17521 ctcccctgct gcttttgctg ctgtgatttc tttttctgtc ggatcaatca gttgagtcga
    17581 ggatgtggtt gaggttgtct cgttgttgct agactgattg gtgctttcta tggactgtat
    17641 ttcagaatag ggtggactat ttgtctcctc tgtcgtttgg ttagcaaaat ttgacgtttc
    17701 tgtataaacg tttacataaa tacttgataa gctccctaag aaagtaaaaa tcaagatgat
    17761 cgaaatcata atgggatgta agtattttct tttagataca gctttgagtc tagaaacaaa
    17821 atgtttttga catatacgtt ggattttatt ttgagttttc ttaatcattt tcatccctcc
    17881 tctcaataaa aaatcatcaa ataatccttt acacgttatt ctaaatttaa tttttattat
    17941 tttattgatg tatagaaagt ttggggcttt ttttctcttt ttattctaag gttattttag
    18001 tcttgtcctt taatacaaag attttatttt taaataaaaa atactctttt tttacgatta
    18061 ttgaaaaaaa aagataaaac ttgattaatt gaagtattgc ttaaatttgg aagctatgag
    18121 ggaagaataa aagagttagg acgggattat ctactatttt tctttgtttt aaatatttgt
    18181 tatctttgaa cataaaagag aaatgtgtcg actgcagtgt tgtgtccctc atcacgaata
    18241 aacggtgttc aattagctga tcttgtattc acttaaaaaa tggtaaagta cgagttgcta
    18301 ttccccattt tcaattttct aacgctcagg acaaaatact gttttcacaa cctcattgga
    18361 ataaaagcat atatattgtt tcaagaacga aatcttgtta tctttttaaa gggcatctgt
    18421 ccaaaatcta attttaggag gtacgttttg gtatttattc gtacaaataa aagagaatta
    18481 tctttattca tgtaacgcat ccaattgatt ttttcatgta cgctcctgga acgattttac
    18541 tgacgattgc ttatttaact gcgaatcgct cattagcttt tatcatagct atccatattt
    18601 tgaataacgt tcttggcttt gtattataga tacagatgac aaaaaaagtt caagtatgcg
    18661 gtgtagaacc aatatcgcat acttgaactt ttttcaatga gtataaatta aaacttctaa
    18721 ttttcaattc ccatcatctg atatgcccga ccgtctgaac ttcttttgaa aaagccaaat
    18781 gtgattagat aacgtctttg ttcgacataa tcgccactag aaaatacgtt cagcagtatc
    18841 gaattaacct ctgtttctgt ataagtcact ccatggtcaa agcgtttcaa gacttctttc
    18901 aataggatgt atttcttttt tgttgaactt ggtacttgtt ttacttttcc atcattcatg
    18961 aatgtgtcta aaactttcat cttttcttgc tccgtataag tataaaccat cttctttctc
    19021 cttttgattc taagaatact ccatttatgt tagaatattc tggatttttt attaaagcgg
    19081 ttactcttaa gaaagatatt atcatagtgc tctttttttt actactactt ataggtagat
    19141 ttattctaat ccattattct cccctaccaa taattaaaga aaaaactgga tcagggtgtt
    19201 caaattacat gctaa
//
LOCUS       DACDIH010000048        19050 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDIH010000048 DACDIH010000000
VERSION     DACDIH010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.048"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..598)
                     /locus_tag="IV281_001835"
     CDS             complement(<1..598)
                     /locus_tag="IV281_001835"
                     /inference="COORDINATES: protein motif:HMM:NF013539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8780823.1"
                     /translation="MFNFDQERFLKTQAGAVATNEQISNILESIASDLEEIYFIGSGG
                     VGILFDSTIEFARTRSTFPFHRVIAAEFMIHPNKTFSNKSLVVIPSLSGTTKETLELV
                     EYCNEMNVKTLALVGSPKTPLEKAATYSIYNDVLDDTSSENYYLQGLLIALFLMKQRN
                     EIDINQYNTYIEDLKKIPEALVHAKEQAEPMAKSFAKKH"
     gene            788..3511
                     /locus_tag="IV281_001836"
     CDS             788..3511
                     /locus_tag="IV281_001836"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012385.1,HMM:NF013070.1,HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8780824.1"
                     /translation="MKTKILKELNDLLNSYDFPQYFPEECNAQYIAQKFQIKRNTASK
                     HLNELMEQKKVVKINGRPVLFFPVHTIERYISHSLDSFEFSSIDSFREKISKKKADHF
                     FDDLIGAKQSLKEAVQQGISAMKYPGGLPIMYFGSSGVGKSYLVEKLAEYCRQTGIIH
                     ENAPFIELNCAQYFNNPELLTSQLFGHMKGSFTGADSDHIGIIEAADGGIVFLDEIHR
                     LPPEGQEKLFIHMDKGVFRRVGDSGPWRLSHVRYIFATTEQEENFFLETFRRRIPIVC
                     TLPNYQDREISERKEIVFHLLEKESTLINKEILVSEAALSFLIRLDLYGNIGELDGLI
                     KQTIANKLFSGSNKKYIEIHISDLPSRYLLTENIELKNQIASQRFYIFSNGNYVHDCD
                     ETSTVTTNLRGLIHRVSKYYSECHDVNRNSEEFRRFVKDQMAEFNDRFIYQKNSEVLV
                     KYYLPEIQNIVQKISPSLFVHLSGNTVQAIATYLAIRDQWGYGKENASANEIEKLYEN
                     LKCNYLTDLNVFLLIFENALDTTLTIWDKLYISLCVISETGNEAKDINAIILAHGYAT
                     ASSIANVANRLVNQFVIDSIDMPLDISFSEVIDRLLHYIEYRKPKDGLVIFVDMGSLA
                     QIKTEIEQVIEVPTMIINNVTTEMAIETAQLIQSTSDIQKVVKKLPYSQFEKQVLYPI
                     KIRKRTIVVSCNTGLGTSIKIKEMMENNLSKDLGIEFLPYENETLRDTQQLEFLIKTK
                     DIIAIIGTDDPRVSNIPYISLEELFDGEIGKLYKVLLRVYGKEIAEAINQNIVKNSSL
                     NRLIDSLTILDAEKVVNQIEESLKDYQMRVGKTLSSLSKINLYVHISCMIERLIRNQG
                     VSSYPAIDQFLKRYETEVASIQKALSKIEKLYNIKIPMEEIAYVCNIIVSY"
     gene            3639..4061
                     /locus_tag="IV281_001837"
     CDS             3639..4061
                     /locus_tag="IV281_001837"
                     /inference="COORDINATES: protein motif:HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose IIA subunit"
                     /protein_id="HAP8780825.1"
                     /translation="MRQILCMSHGPLAKGMIETLSIIVGDLDNIDFRCAYVDGNNDVE
                     MIIDDYLKESMGNETIVVTDIFGGSINNEWLRRLPDLSKVYLISGMNLSLLVDLYLKF
                     QLVSSDQIEEIIDHAIQASSGSIKFCNQLDYEQTVDDF"
     gene            4079..4549
                     /locus_tag="IV281_001838"
     CDS             4079..4549
                     /locus_tag="IV281_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8780826.1"
                     /translation="MIKLVRIDHRLIHGQVALTWTNAISADCILVANDEILNDPIQKT
                     TLKLAAPHNVKVVIKSIQDSIDAINSGKTDKYKLFVVTKTISDAKRLANGVEQIKSVN
                     IGSVKETSGSSALTHTIFVTEEERAELKGMVSEGIEVFAQQVPTDKAIDLKKSL"
     gene            4573..5355
                     /locus_tag="IV281_001839"
     CDS             4573..5355
                     /locus_tag="IV281_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8780827.1"
                     /translation="MDSLLVQAILLGLLTAFIVIDWMTGTNNLSRPIVSSVFVGLIMG
                     DLKMGIMMGATLELAFIGAITIGAARPPDTVSGGILGTAFAIATGKGAEFALTLAFPI
                     AALFLLVDNLLTLLVLPMIARKADRYAEKGELKNVSKMHVLGFILVKSLPRALFVGFS
                     FYLGSPVMERILNAIPTFVQDGINIAAGIMPALGFAILLQMLLKKDVFVYFVIGFALF
                     SYLHVPILGIAIFGACLAYIIVNMETKAAMGSRGEEMVEDDF"
     gene            5345..6169
                     /locus_tag="IV281_001840"
     CDS             5345..6169
                     /locus_tag="IV281_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8780828.1"
                     /translation="MTSSGKALTKDDLTKICIRSCALEASWNFERQQHMGFAFAMMPA
                     LEKIYSGNEEEKQKAAQRHLEFFNTAPPLSTFIMGIVASMEEENAKNPDDFDTSTITS
                     IKTALMGPLAGIGDSMFWGTLLVIGIGVGASLAKEGNILGPFLFLLIFNVPNFLLRYF
                     GVMQGYNFGTKLLENIESSGVMKTLTHAASILGLMVIGGMSGSMVSIKVAGSIGQGDE
                     ATKIQSIIDGILPNLLSVLCVFLIYWMLKKGFKTTYVLFGIFIFGIIAAFFGFLTT"
     gene            6204..7202
                     /locus_tag="IV281_001841"
     CDS             6204..7202
                     /locus_tag="IV281_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8780829.1"
                     /translation="MLNYNAKEMKKKGKRIYGIREDVEKIAKQAYDSGISNIFFTASG
                     GSVAVMQPFEYVLMTESSIPVYSMTAAELVLMGHKQLNEKSLVILYSKSGDTKETIKA
                     AEYLKERNVPTVGIVSKEDTPLEALSTYAINYLDGRPQEMPLYFLIMSIMNLNGEFPE
                     YTRFADELKNLPTALNSVREQADEKGKKYAEEFKEDPYQIWIGSGNLWGITYSYSMCV
                     LEESQWLRTKSVSSPEFFHGTLELVEKGVCVTLLKTEGETRLLDARVEKFAVQYTDKF
                     NVFDTKDYELPGISDEFRKYFCAPVMWAALGRVSVHLEDIRNHSLDKRRYYRVVEY"
     gene            7219..8073
                     /locus_tag="IV281_001842"
     CDS             7219..8073
                     /locus_tag="IV281_001842"
                     /inference="COORDINATES: protein motif:HMM:NF012515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780830.1"
                     /translation="MTAYVIGFGDNVVDYYENLNQKFPGGNAVNFAVNAKKSGVKAYY
                     VGSLSSDPDGVLLQHAISSEGVDISFSEVIDEPTEQAHVTIKDGDREFIGGVRGSRLT
                     PALSAELIDLMKNSSLVHTSCHSRTETKIKELKKYDVKVSFDFSDLDKYRTHEYLSDV
                     CPSIYIAQFSVATDDQEEINRLISECKDFEVPYLLFTRGSKSPVFVDTRENKTYEGFI
                     RKVESPHDTMGAGDAYFAAFASNFVLSKREKSMIEHVIDCFHKGANLAYETLMIDGSF
                     GHATPINP"
     gene            complement(8647..9897)
                     /locus_tag="IV281_001843"
                     /pseudo
     CDS             complement(8647..9897)
                     /locus_tag="IV281_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            10414..10497
                     /locus_tag="IV281_001844"
                     /pseudo
     CDS             10414..10497
                     /locus_tag="IV281_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            10586..11997
                     /locus_tag="IV281_001845"
     CDS             join(10586..11141,11141..11997)
                     /locus_tag="IV281_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8780831.1"
                     /translation="MAKYPFELKLKIVKEYLSGVGEYRHLANKYGISSKSQVSDWVNA
                     YREYGEEGLLKKHQNQKYSVQFKLNALELYQTSEMSYREVASTLEMNNPALIANWMRN
                     FREAGIEGLSKEKGRPLTLTRKKENKKENTTTEERERIKALEKQVRSLQIENAFLKEL
                     RKLRKQEAQQRRKNQSHESSPASEDPFKLVELLETLKFPKATYMYWQKRFDRPNPDQE
                     IEEKMLEIRKEHKDYGCLRLKKELENQGIHVNKKKIQRLIKKLGIEVKSYTRKSRRYN
                     SYHGKVGTVAKNRIHRRFYTNICHQKITTDTTEFKYYEVDTTGVVRQKKLYLDPFMDF
                     YNSEILSYRISEKPNALAIMEGLEEAIQMTNDCPFRRTFHSDQGWAYQMNAYKNKLKQ
                     HKIFQSMSRKGNCLDNSPMENFFGLLKQEIFHGEVYRSLDELKTKIDQYIYYYNHKRI
                     KKKLNWRSPVQFREAVKTAV"
     gene            12072..13355
                     /locus_tag="IV281_001846"
                     /pseudo
     CDS             12072..13355
                     /locus_tag="IV281_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(13571..14383)
                     /locus_tag="IV281_001847"
     CDS             complement(13571..14383)
                     /locus_tag="IV281_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8780832.1"
                     /translation="MGDSKLTKKEINKVFWRSFALQGSESYTDMQGMGYSFSILPALN
                     KIYQGNDEKYYEAVYRNTEFFNTTPHISTFIMGMSLALEEENAKTDQFETETISSIKA
                     ALMGPVAGIGDSFYWGTFRVIAAGIGLSLAAQGNILGPILYFSIYTLLHFLGRYGCLR
                     IGYRMGANAIEKAFENNYLESFSLAASIVGLMSIGAMTSSLVRFTIPAVFKSKGGEIN
                     VQQMLDDIFPGILSLGLTFFVYFLLKKKVKVLWIILAIFVFSIVGVYIGLLG"
     gene            complement(14400..15131)
                     /locus_tag="IV281_001848"
     CDS             complement(14400..15131)
                     /locus_tag="IV281_001848"
                     /inference="COORDINATES: protein motif:HMM:NF015566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8780833.1"
                     /translation="MIECILIGIVGYLTTIDERYFGASMMNRPIIVGPVVGLILGDLH
                     QGILIGAALEAMFIGIVTIGAALPPDVGVAGTIATALAIKSGAGADVAVTLAMPFAVL
                     AQGLNMLAFTINSFTVSQGRKAIENRKLSSFQKWHFFPLIVRLPSAVLTFLVVYFGAN
                     ATESFVNIMPEKLMTGFTVASGLLPAVGFAMLIQGMIDKKSVPYFFIGYLLAAYLGVP
                     VVGVAVAAIMVALLVIQTKRNPVNI"
     gene            complement(15148..15603)
                     /locus_tag="IV281_001849"
     CDS             complement(15148..15603)
                     /locus_tag="IV281_001849"
                     /inference="COORDINATES: protein motif:HMM:NF015769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8780834.1"
                     /translation="MIKLLRIDDRLIHGQVAMAWTKSVSADYIIVVDDASANDKMKQM
                     ILSLAKPVSTGLDIITSADFLSTFEKRKNQNLMIVTSSPKSAFEIVNAIPGEVDSINI
                     GGLRYAEGKEKVNEYVAITSEDKNYLAKLKESGFKLIMQATPTGKAKIY"
     gene            complement(15596..16006)
                     /locus_tag="IV281_001850"
     CDS             complement(15596..16006)
                     /locus_tag="IV281_001850"
                     /inference="COORDINATES: protein motif:HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8780835.1"
                     /translation="MYTIILASHKNLAKGMKETVEYILGPAPFIKVMTAYTDDNYDIE
                     KDTNILLDGTDKVLVITDLLGGSVNNHWMNYVYEKKLTKKITVIAGMTLSLIMELSMN
                     IEDYKLREKISMIIAESQKSIINCSELMEVEDND"
     gene            complement(16537..17496)
                     /locus_tag="IV281_001851"
                     /pseudo
     CDS             complement(16537..17496)
                     /locus_tag="IV281_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            17930..18116
                     /locus_tag="IV281_001852"
                     /pseudo
     CDS             17930..18116
                     /locus_tag="IV281_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252699.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(18357..18572)
                     /locus_tag="IV281_001853"
     CDS             complement(18357..18572)
                     /locus_tag="IV281_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP8780836.1"
                     /translation="MKKFIIPCIFLFLSILLFSTSILVGSYLDSSGMLIEPAFFCVPL
                     GYLTLGLAFFTALYSFVKQRLLKKYSA"
BASE COUNT         6287 a         3062 c         3669 g         6032 t
ORIGIN      
        1 tgtgtttttt tgcaaaagat tttgccattg gttccgcttg ctctttagcg tgcactaagg
       61 cttcaggtat tttttttaaa tcctcaatat aggtattgta ttgattgata tcgatttcat
      121 ttctttgttt cattaagaag agagcaatca gcagtccttg aaggtaataa ttttctgaag
      181 atgtatcatc taaaacgtcg ttatagatag aatatgttgc agctttttct aggggcgttt
      241 taggacttcc taccaaagct aatgttttga cgttcatttc attacagtat tcgactaact
      301 caagtgtttc ttttgttgta ccagataaag atgggataac aaccaaagat ttgttagaaa
      361 atgttttatt gggatgaatc ataaattcgg cagcgataac acgatgaaaa gggaaagtgc
      421 tacgagttct cgcgaattcg attgttgaat caaaaagaat accaactcct ccagacccaa
      481 taaagtaaat ttcttctaag tcagatgcga tggattctaa aatattggaa atttgttcat
      541 ttgttgcaac ggcaccagct tgagtcttta aaaatctttc ttgatcaaaa ttaaacataa
      601 aaatctctcc tttgattacc aatggtaata ttttgtttgc ataaataaaa taccacaggt
      661 aataaaaagt gtcaaatatt ttttaggaaa aataccatag gtattgttaa agtggaaaga
      721 tgtttataaa gaatattgaa ttttatagtg aaaatcattt attattaagt aaaggaggtg
      781 ggttcatttg aaaacgaaaa ttttaaaaga attaaatgat ctacttaaca gctatgattt
      841 tccacaatat tttccagaag aatgtaacgc gcaatatatt gcccaaaaat ttcaaataaa
      901 aagaaatacg gctagtaagc atctaaacga actaatggaa cagaaaaaag ttgtgaaaat
      961 caatggtcgt cctgttcttt tttttccagt ccatacaatt gaacggtata tatcacattc
     1021 attagatagt tttgagtttt cttcaattga ttcgtttaga gaaaaaattt caaagaaaaa
     1081 agcagatcat ttttttgatg atttaattgg tgcaaaacaa agtttaaaag aagcggttca
     1141 acaagggata tcagcaatga aatatccagg tggattacca ataatgtatt ttggatcgtc
     1201 cggagttggg aagagttact tagtggaaaa actagctgag tattgccgac aaactggtat
     1261 tattcatgag aatgctccgt tcatcgagtt aaactgcgct cagtatttta ataatccaga
     1321 gttattaaca agtcagttgt ttggccatat gaagggatcg tttaccggcg ccgattctga
     1381 ccatattggt ataatagaag cagcagacgg tggaatcgtc tttcttgacg aaattcatcg
     1441 tttaccacca gaaggacaag agaaactttt tattcatatg gataaaggcg tttttcgaag
     1501 agttggagat agtggtcctt ggcgtttatc acatgttaga tatatttttg cgacaacgga
     1561 acaggaagaa aatttttttc tagaaacttt tcgacgaaga attccgattg tttgtacttt
     1621 gcctaattac caagatagag aaattagcga acgaaaagag attgtcttcc atttattaga
     1681 gaaagagtct actctgatca ataaagaaat attagtttct gaagccgccc ttagtttcct
     1741 gattcgctta gatttgtatg gaaacattgg tgagctggat ggactaataa aacaaactat
     1801 tgcgaataaa ctatttagtg gaagcaataa aaaatatatc gaaattcata tttctgattt
     1861 acctagcagg tacttgctaa cagaaaatat tgagttaaaa aaccaaattg cgagccaacg
     1921 gttctatatc tttagtaatg gtaactatgt tcatgattgt gacgagactt caactgtaac
     1981 tacaaattta cgaggtttga ttcatcgtgt ttctaaatat tattcagagt gtcatgatgt
     2041 gaatagaaat agtgaagagt ttcgacgttt tgtgaaggac caaatggcag aatttaatga
     2101 tcgttttatt taccaaaaaa attcagaagt cttagttaag tattatttac cggaaatcca
     2161 aaatattgtt caaaaaatct ctcccagtct ttttgttcac ctttctggca atacggtcca
     2221 agctatagct acctatctcg ctattaggga tcaatggggg tatgggaaag agaatgcttc
     2281 agcgaatgaa attgaaaagt tatatgaaaa cctaaagtgt aactatttga cggatttaaa
     2341 tgtattctta ctgatcttcg agaacgccct ggatacaaca ttgactattt gggataaact
     2401 gtatatcagt ttgtgtgtta ttagtgagac cggaaatgaa gcgaaagaca ttaacgcaat
     2461 tattttagcc catggatatg cgacagctag tagtattgct aatgttgcca atcgtttggt
     2521 taatcagttt gtcatagatt ctattgatat gcctctagat atcagcttta gtgaagtaat
     2581 tgacagatta cttcactata tagagtatcg aaagcctaaa gatggactag tgattttcgt
     2641 tgatatggga agtttagctc aaattaagac tgaaattgaa caagtaatcg aagttccaac
     2701 aatgattatt aataatgtta caaccgaaat ggctattgaa accgcacaac tgattcaaag
     2761 tacgagtgat atccaaaagg ttgtaaaaaa attgccgtat tctcagtttg aaaagcaagt
     2821 tctatatccc atcaaaatta gaaaacgtac aatcgttgtt tcctgcaata ctggtttggg
     2881 tacctccata aaaattaaag aaatgatgga aaataatttg tcgaaagact tgggcattga
     2941 atttctgcca tacgaaaatg aaactcttag agatactcag caattagagt ttctcattaa
     3001 aacaaaggat atcattgcca ttatcggaac agatgatccg cgtgtgtcga acattcccta
     3061 tatttctctt gaagaattat ttgatggtga aattggcaaa ctctataaag ttcttttgag
     3121 agtctatgga aaagagattg ctgaggcaat taatcaaaat attgttaaaa atagctcatt
     3181 aaaccgcttg attgacagtc ttactatttt agatgccgaa aaagttgtca atcaaattga
     3241 ggaaagttta aaagattatc aaatgagggt ggggaaaacg ttatcatcat tgtccaaaat
     3301 taatctttat gttcatatca gctgtatgat tgagcgacta attcgtaatc agggagtaag
     3361 tagttatcca gcaatagatc agtttcttaa gcgatacgaa actgaggttg cttctattca
     3421 aaaggcactt agtaagattg agaaacttta taatattaag attcctatgg aagagattgc
     3481 atatgtgtgc aacatcattg taagttattg ataaaataaa gtgacactat tcacagcgat
     3541 ggaaatagtg tcactttatt ttttgtacgc cctttcattt aagtggtgac agtaattggc
     3601 acagtatttg ctaataacaa agcgaaagga gttcgggtat gagacaaata ttatgtatga
     3661 gccacggtcc tctagcaaaa ggaatgattg aaactttgag tattatcgta ggagatttgg
     3721 ataatattga ttttcgatgt gcctatgtcg atggaaataa tgacgtagaa atgatcatcg
     3781 atgattatct aaaggaatct atgggaaatg agacgattgt tgtaaccgac atttttggtg
     3841 gaagcattaa taatgagtgg ctaagacggt tgccagatct ttctaaagtc tatttgattt
     3901 ctggaatgaa tttaagtctt ttagtggatt tatatttaaa attccaatta gtttcatctg
     3961 atcagattga agaaattatt gatcatgcaa ttcaagcatc ttctggttcg attaagtttt
     4021 gtaatcagct agattatgag caaacggttg atgactttta aataaaggag ggaagagaat
     4081 gattaaatta gtgaggatcg atcatcgttt gatacacggt caagttgctt taacttggac
     4141 gaacgcaatc agtgcggatt gcattttagt agcgaacgat gaaattttga atgatccaat
     4201 tcaaaagaca actttgaaat tagccgcacc acataacgtg aaggttgtta tcaaatcaat
     4261 tcaagattcg attgatgcaa ttaatagtgg gaagacagat aaatacaaac tatttgtagt
     4321 taccaaaacc atttccgatg ctaaacggtt ggcaaatggg gttgaacaaa ttaaatctgt
     4381 aaatatcggt agtgttaagg aaactagtgg cagtagcgct ttgacgcata caatttttgt
     4441 aacagaagag gaacgagcag aacttaaagg aatggtctca gagggcattg aagtgttcgc
     4501 tcaacaagtt ccaacagata aggcaataga tttaaaaaaa tctttgtaat aaaaaaataa
     4561 gggggtaatt tcatggatag tctcttagta caagcaattt tgcttggtct cttgactgcg
     4621 ttcatcgtaa ttgactggat gacaggtacg aataacttat caagaccaat cgtatcttcc
     4681 gtctttgttg gtttaattat gggcgatctt aagatgggga ttatgatggg ggcaacactg
     4741 gagttagcat ttatcggagc aataacaatc ggagcggcac gtccgcctga tacggtatct
     4801 ggtgggattt tagggactgc gtttgctatt gcaactggca agggagcaga atttgcgttg
     4861 accttggcat tcccaatcgc tgcgctattc ttattagttg ataatctact aacattgctt
     4921 gttctaccaa tgattgctag aaaggcagat agatatgctg aaaagggcga actaaaaaat
     4981 gtgtcgaaaa tgcatgtttt aggttttatc ttagtaaaaa gtttaccacg ggctttattt
     5041 gtcggattct cattttactt aggaagtcct gttatggaac gaatcttaaa tgctattcca
     5101 acttttgtcc aagatgggat taacattgct gcaggcatta tgccagcact aggttttgct
     5161 attttattgc aaatgcttct taagaaagat gtctttgtat atttcgtaat tggttttgca
     5221 ctctttagct acttacatgt gccgattctg gggattgcaa tcttcggcgc atgtctagct
     5281 tatattatag taaacatgga aaccaaagct gcaatgggtt caagagggga ggagatggtt
     5341 gaagatgact tctagtggga aagctttaac taaagatgat ttaacaaaaa tttgtattcg
     5401 gtcatgtgca ttagaagcat catggaattt tgagcgccaa caacatatgg gctttgcttt
     5461 tgctatgatg ccagcgttag aaaaaattta tagtggcaat gaagaagaaa agcaaaaagc
     5521 agctcaaaga catctagagt tttttaacac agctccgcca ttatcaacat tcataatggg
     5581 aattgtggct tctatggaag aagaaaatgc caaaaatcct gatgactttg atacttctac
     5641 aatcacttct attaaaactg cattgatggg accattagct ggtattggtg attcaatgtt
     5701 ttggggtacc ttattagtta tcggtatcgg tgtaggtgct tcattagcaa aagaaggcaa
     5761 tattttgggt ccatttttat ttttactaat cttcaatgtc cctaattttc ttctccgtta
     5821 ctttggagtt atgcagggat ataactttgg tacaaagcta ttagagaaca ttgaatcctc
     5881 tggcgtcatg aaaacgttga ctcatgctgc ctcaattttg ggtcttatgg taattggggg
     5941 catgtctgga agtatggtat caatcaaagt agctggttct attggacaag gtgatgaagc
     6001 aacaaaaatt caaagtatca ttgatggtat tttaccaaat ctgctgtcag tattatgtgt
     6061 atttctgatt tactggatgc ttaaaaaagg ctttaaaaca acttacgtgt tattcggaat
     6121 ctttatcttt ggtatcatag cagccttctt cggatttttg acaacttaat tgaataatct
     6181 aaataattgt aaggagagaa ctaatgttaa actataatgc aaaagaaatg aaaaaaaaag
     6241 gcaaacggat ttacggtatt cgtgaagatg tagaaaaaat tgcaaagcaa gcttatgatt
     6301 ctggaattag taatattttc tttactgctt ctggtggatc ggtagccgta atgcaaccat
     6361 ttgagtatgt attgatgact gaatcaagta ttcctgttta ctctatgact gcagctgagc
     6421 ttgtcttaat gggacataaa cagttgaatg aaaaatcttt agtaatctta tactcaaaat
     6481 ctggagatac gaaagaaact attaaggcag ctgaatattt aaaagaacga aacgtgccaa
     6541 ccgttggtat tgtaagtaaa gaagatacac cgttagaggc tctatcaact tatgcaatca
     6601 attacctaga cggccgccct caagaaatgc cactttattt cttaattatg tctatcatga
     6661 atttgaatgg ggaatttcct gaatacactc gttttgctga tgaactgaaa aacttaccaa
     6721 cggctctgaa ttcagttaga gaacaggctg atgaaaaagg gaagaagtat gccgaagaat
     6781 ttaaagaaga tccttatcaa atttggattg gtagcgggaa tttatgggga atcacctact
     6841 cttattcaat gtgtgtgctt gaggaatctc aatggttgcg tacaaagtct gtctcatcac
     6901 cagagttttt ccatggtacc ctcgagttag tcgaaaaagg agtttgtgta acacttttga
     6961 aaactgaggg ggaaacacgg cttttagatg ctcgagttga aaaatttgcg gtccaatata
     7021 cagataaatt taatgttttt gatactaagg attatgagct accaggtatt agtgacgaat
     7081 ttcgaaagta cttttgtgct ccagttatgt gggcagccct aggtcgcgtc agtgttcatc
     7141 ttgaagatat tcggaaccac tctttggata aacgtcgtta ttaccgagta gttgaatact
     7201 aaaagtaggt gattaattat gacagcgtat gtgattggat ttggcgataa tgttgttgat
     7261 tactatgaga atttgaatca aaaatttcca gggggaaatg cagttaattt tgctgtgaat
     7321 gcaaaaaaga gtggtgttaa agcatactat gtcggtagtc tatcatctga tcctgatggc
     7381 gtgttattac aacacgccat cagttctgaa ggtgttgata tctctttctc ggaagttatt
     7441 gacgagccta ctgagcaagc acatgtaact attaaagatg gagataggga gtttataggt
     7501 ggcgtgcgtg gctcccgatt gacacctgct ctctcagcag aacttattga tttgatgaag
     7561 aacagttcat tagttcatac tagttgccat agtcgaacag aaacaaaaat taaagaatta
     7621 aaaaaatatg atgttaaagt ttcttttgat ttttctgact tagataaata tagaacgcat
     7681 gaatacttgt cagatgtttg tccgtcaatt tatattgcac agttttcagt cgctacagat
     7741 gatcaagagg aaattaatcg cttaattagt gaatgcaagg actttgaggt tccgtatctt
     7801 ctttttacgc gtggtagtaa gagccctgtt tttgttgaca ctcgagagaa taagacttat
     7861 gaaggattca ttcgcaaagt tgagtcacct catgatacaa tgggcgcagg tgatgcatac
     7921 tttgcagctt ttgcaagtaa ttttgtctta tctaaacgtg aaaaatcaat gatagaacat
     7981 gttattgatt gttttcacaa aggtgcaaac cttgcatatg aaacattgat gattgatggc
     8041 tcctttggtc atgctacacc tattaaccct tagctttgtg ctttgggctt ttattgctgg
     8101 aatttgtata gtagactcat cataactata tttttgtatg tgaaaaggca agaggctatg
     8161 atgaaaataa cactggctaa ttgcatgagg gagcatatcg ttgttaaagc gtcattgata
     8221 gtatgaataa atatctcgat aatattatat tgttatttac ttaatttatt gattagtcaa
     8281 gcaaagataa tcttctgtta cttaacgaga ctagtaggta caagcacttc atgttatgta
     8341 cgacccaaaa atattgctgt tgattatttg ctgaaattat tgcgagcttc aatttatttt
     8401 taaaggactt ggtaggctgc gtgatgaaat aagacttgga aggctgtcta aaccaatata
     8461 tagtagacta aatagtaaaa aagagcatgg taattgtctc tttgtcaata agtctaatgg
     8521 taataagaca agttggagat tgagaatatt tttattgtgt aacctatttt gaagcgtact
     8581 taactagccc cggccaaaat caaatttttt cggggtatat caagggtttg cgtcaataac
     8641 agaatgttat ttatctatca cgttccttgg tggcgtgtag agttgatggt tacgtagtag
     8701 cgtatctacc agacgcgtaa attttcttgc ggttaagacg tgtgctcgtt tgtgtttatt
     8761 tttgggacct ctttacattt gctttgataa aaagctttat attgaggaag atattttttt
     8821 acagagttgg aagcttcaac taagtagtat ctaaaatagc gactcctttg ttttgtcaga
     8881 ggtgtatttt gtgaccgata gttaacagat tgtttgcctt tccaacataa gcctgcgtat
     8941 tttgccaatt ttgtttgatc ttcaaatcgt tcaatttgac cgatttcagc gattaatcca
     9001 gcggcgtgta cctttccaat acctggaaca ctagtcaaat attgatattc tggaataacg
     9061 acaacaaatt gctcggttgc tttatccaag tctttgatgt tcttttcaag cgcacgaatt
     9121 tcacatacaa aaacatttag aacgacattg atggattctt atgcaagact atctaaccga
     9181 taactcacgg caattgcacg ttgaatcgat ttggcatttt tttctggatc tttaaagcga
     9241 catttccttt ttcttttagt aagtcacaga aagcttccaa aggaagcaca gccaattcat
     9301 ctagcgtgaa gtcttctgtc acaagagtaa tgacagtggc actatagaga cttgtcgatt
     9361 catcgcgcat ttatcgagta agcgtattgc atttgtacgt tagattttcc agaaagtgtt
     9421 gttttgtacg agtgaagttg cttaatgagt cgatagcgag tgcgtgttaa acgctgtaaa
     9481 gccacgtact tctcttcttt gataggagaa gttgtgaacc gttgaatacg caataaatca
     9541 gcaattctta acgcgttgtt tcgatcagtc gtatcttcct caaacatgcg actgaattgt
     9601 ttcatccgaa aaggattttc aatcctcaca agtgcattga gtttttttag ctctgcatct
     9661 tcatggaaaa acatcgaagg atgaaagctg tacatggacg tttaattcca tctcaataac
     9721 gatttgaccg aagtgattgt tttcattgaa cacaagaatc agttctcgtg tcaatggagc
     9781 cctttcaatg tcattagcaa cagaattttc agtcaatata gatagctgat cgtcgtctgt
     9841 taggaagcaa acgtctaatt tttcagaact aacgtcatta ccaacaaata atttcatgga
     9901 ataaaaacct attttcgata taaattctag aaaatgcttc gatactgtgg gattccacag
     9961 aaactcactg tgtggtcacg acctcgccta atagaatacg gtaataaaag agctaatgag
    10021 ttgatagtaa acacagcgct ttggataaca gacttatgaa ggtagacaaa gctacaggac
    10081 tgattagcaa attctcgttg aatcctttcc atacatccat tgtactctga gaaatcacac
    10141 aataacgaaa taaattccgg aaggaatatg ttattcaaaa aaatttgtgt tttatagata
    10201 ctttaaaaga aacttattag gcaaaatata ttttacgaag actgatgagt tgtgcaaaat
    10261 caaatctgag tcagaatgaa cttcattcta gctcagattt ttgtttaatg acttcagtca
    10321 gtttcttgca ttacgtgaat gaaaatggga ctggtgatgt ccaaacccta aaaagttaga
    10381 ccaaaatcta acttcagggg ggatggtatt tttatgggaa aatatagctt tgaattcaag
    10441 tcaacaatcg ttcaagagta tcttgaagga gaaggtggat taggatattt agcaaaaata
    10501 caaggagtga gatcaaaaaa aggtacacgg atggatcaat caaaagttag accaaaaaat
    10561 ctaacttttg ggggtcgtta tttgtatggc aaaatatcct tttgaattaa aactaaaaat
    10621 cgttaaagaa tatcttagtg gcgtgggcga atatcggcat cttgccaaca aatacggtat
    10681 ttcaagcaag tctcaagtaa gtgattgggt gaatgcctat cgtgaatacg gagaggaagg
    10741 tctattgaaa aagcatcaaa atcagaaata ttctgttcaa tttaagttaa atgcgctaga
    10801 gttatatcaa acaagtgaga tgtcctatcg agaagttgcc agcactttag aaatgaacaa
    10861 ccctgcgctt attgccaatt ggatgcgtaa tttcagagaa gcaggtattg aggggctctc
    10921 aaaagagaaa gggcgaccac ttactttgac tagaaaaaaa gaaaacaaaa aagaaaatac
    10981 aacaactgaa gaacgcgagc gtatcaaggc attagaaaag caagtccgat cccttcaaat
    11041 tgagaatgcc tttttaaaag agttgaggaa gctgcgaaaa caggaagccc agcaaagacg
    11101 caagaatcaa tcgcacgaat catcgccagc ctccgaggat cattcaaact agttgagctt
    11161 cttgaaacat tgaaatttcc taaagcaacc tatatgtatt ggcagaaacg gtttgacaga
    11221 ccaaacccag accaagaaat tgaagaaaaa atgcttgaaa ttcgcaagga gcataaagac
    11281 tatggctgtc tgcgactaaa aaaagaatta gagaatcaag ggattcatgt caataaaaag
    11341 aagattcaac gtctgataaa aaaactaggt atcgaagtaa agtcttacac tcgaaaatct
    11401 cgacggtaca attcttatca tgggaaggtt ggcactgtcg ccaaaaatcg tattcatcgt
    11461 cggttttata caaatatctg tcaccaaaaa attacaactg ataccacaga gtttaagtat
    11521 tatgaagtag acaccacagg tgtcgttcgt cagaaaaagc tgtatttaga tcctttcatg
    11581 gatttctaca atagtgaaat tctatcttat cggatttctg aaaaaccaaa cgcactagcc
    11641 ataatggaag gattagaaga agcaatccag atgacaaatg attgtccatt tcgtagaacc
    11701 ttccattcag atcaaggatg ggcctatcaa atgaatgctt ataagaataa attgaagcag
    11761 cataaaatct tccagagtat gtctcgtaaa gggaattgtt tagataactc acctatggaa
    11821 aacttctttg gtttattaaa gcaagagatt tttcatggag aagtctatcg gagtttagat
    11881 gaattgaaaa caaaaattga ccaatatatc tattattaca accataaacg catcaaaaag
    11941 aaattaaatt ggcgaagtcc ggttcagttt cgagaagctg taaaaacagc tgtctaacag
    12001 ttaattttat cgtacaaaaa aggagtggat ttctccactc cataaaagtc taactttttg
    12061 gggtcgctac aaatgcttat cgagaatttg gagaagaagg cctcttgcgt aagcgaaata
    12121 agcgaagtta ttctgttcaa ttcaagatag atgcgataga gttataaaaa acgggtgagc
    12181 tatcctatcg tgaagtggcg aatcatttag ggataaacca acctgcgtta gttgtcaatt
    12241 ggatgaaaat ttttgttcaa aaggattgca cgggctctca aaaatgaaag ggtcctcctc
    12301 cattttgtct aagaaagaat aaaaggaaga aacaagcaaa ctgacgccta aagagcataa
    12361 tcgtatcaaa gaattggaaa aataagtgcg tttccttcag attgagaata cctttttaaa
    12421 agaattgagg aagcttagaa aacaggaagt ctaacaaaga cgaacgaatc aatcgcacga
    12481 atcatcgaca acctccgagg atcattcaaa ttagttgagc ttcttgaaac cttgaaattt
    12541 cctgaattaa cctttatgta ttggcaaaga cgatttgatc gaagaaattc agatcaagaa
    12601 atcgaagagg aaatgcttaa aataagaagt caatataaag attatggcta tttacgtatg
    12661 acaaaggaat tgtgggaccg tgggttccat gttaacaaga aaaaagtaca acgtttgatt
    12721 cgaaagctga gtattgaagt acgatcattt acgagaaaat atcgccgcta taatacttat
    12781 cgtggaagaa tcggcatgat tacaaaaaat cgaattcatc gacgctttta cacaagcaac
    12841 tgtcatcaaa aggttacaac agatacgaca gaactaaaat attttgaaat ggataaatac
    12901 ggtgccaatc gtgaaaagaa gctgtatctt gatccatttt tagatatgta caacagtgaa
    12961 attttatttt atcgcatttc tgaaaagcca agtgcgttgg ctatcatgga agctttagag
    13021 gaagctatct gcgcaacgga atgtatctat cgtcgaactt ttcactccaa aaaggatgga
    13081 cctaccaaat gaaagcttat agtcataagt tgaaagaaca taagatcttc caaagtatgt
    13141 cccgtaagga gacctgctta gataattcga tcattgagaa cttccttagt ctattaaagc
    13201 aggaaattta ttatggaaga gactatcgta tctttgaaga attgaagcaa gccattgatg
    13261 gctatattca ttactacaac catgagcgta tgaaaaggaa actaaactgg cagagtctgg
    13321 tgcaatttag agtaatagca ttcaaagctg tctaaaatca tcaagaaaag caaagaaaaa
    13381 cggagtgaat tttctccact ccgaaaacat ctatcttttt taggtcacta cagaatcaat
    13441 tctgattcat cagacccata aattatagat cctaataatt tataccatgc tctttttact
    13501 catatttaca tttatctttg ggtattttat ggtttaagtt gatgtctgat ctagttttat
    13561 tctggattaa ttaacctaat aatccaatat aaactccaac aatactaaag acgaagatag
    13621 caagtataat ccaaagaacc tttacttttt tctttaatag aaagtaaaca aagaatgtta
    13681 gccccaatga gagaattcct ggaaaaatat catcgagcat ttgctgaaca tttatttctc
    13741 caccttttga tttaaatacg gcgggaatag taaagcgtac cagagatgag gtcatggctc
    13801 caattgacat taaacctacg atagatgctg ctaacgagaa actttcaagg taattgttct
    13861 caaatgcttt ttcaatggcg tttgctccca tccgatatcc aatacgtaag cagccatatc
    13921 tgccaagaaa atgtaacagc gtataaattg aaaaatacaa tattggacct aaaatgtttc
    13981 cttgggcagc taaagataag ccgatgccag cagcaataac gcgaaatgtt ccccagtaaa
    14041 aactatcacc aataccagct acaggcccca tcaatgcagc ttttatcgat gaaattgttt
    14101 cagtttcaaa ttgatctgtt tttgcatttt cttcttcaag tgctaatgac atacccataa
    14161 taaaggttga aatatgaggc gttgtattaa aaaattccgt atttcggtaa acagcttcat
    14221 aatatttttc atcattccct tgatatattt tgtttaaagc aggtaatata gaaaaactat
    14281 atcccattcc ttgcatatca gtgtatgact cggatccctg taaagcaaat gaacgccaga
    14341 aaaccttgtt tatttctttt ttagttaatt tagaatctcc cataattatc actctccctt
    14401 taaatattta caggattgcg tttagtttgg ataactaata aagcaaccat aattgcagca
    14461 acagctacac caactacggg gactcctaaa tatgccgcta aaaggtatcc aatgaaaaaa
    14521 taaggaactg attttttgtc aatcattcct tgaattagca tggcaaatcc aacagcgggg
    14581 agtaaaccag aagctacggt gaaaccggtc attaattttt ctggcataat atttacaaaa
    14641 ctttctgtag cattggcacc aaaatatact actaaaaatg taagtactgc ggaaggtaag
    14701 cgaacgatta atggaaaaaa atgccacttc tgaaaagatg ataatttgcg gttttcaatt
    14761 gcttttctac cttgtgaaac ggtaaaggag ttaatcgtaa aagccaacat gtttaatcct
    14821 tgtgccaata cagcgaaagg catcgccaat gtaacagcta catcagcacc tgctccactt
    14881 tttattgcta gagccgttgc aatagtacca gctacaccta catctggtgg tagtgcagca
    14941 ccaatcgtta caatgccaat aaacattgct tctaatgctg ctcctatcaa gatgccttga
    15001 tggagatccc ctaagataag tccaacaaca ggtccaacta ttatcggtcg attcatcata
    15061 gatgccccaa aatatctttc atctattgtc gttaaatacc caactatgcc aattaaaatg
    15121 cattcaatca ttcttattcc ccaccttcta ataaatcttc gcttttccag ttggagtagc
    15181 ctgcataatt aatttgaagc cggactcctt taattttgct aagtagtttt tgtcttctga
    15241 tgtgatagcc acatactcgt taactttctc ttttccctcg gcgtaccgta aaccaccgat
    15301 attaattgaa tcgacttccc ctgggatagc gtttacaatt tcaaatgcgc ttttagggga
    15361 actagtgact atcatcaaat tttgattttt ccttttttca aaggtactta aaaaatctgc
    15421 tgaagtaata atatctagtc ccgttgatac aggttttgct aatgataaaa tcatttgttt
    15481 cattttgtca ttagcagagg cgtcatcaac tacaataatg tagtcagcag atacggactt
    15541 agtccaagcc attgcaactt gaccgtggat caggcgatcg tcaattctta atagtttaat
    15601 cattatcttc tacctccatt aattctgaac agtttataat gctcttttgg ctttcagcaa
    15661 taatcatgga aattttttct ctcaatttat aatcttcaat gttcatactt aattccataa
    15721 ttaaggacaa agtcatacct gcaattactg taattttttt tgttaatttt ttttcgtaaa
    15781 cataattcat ccaatgatta tttacgctac caccaagtaa atcagtgatg acaaggactt
    15841 tatcagttcc atcgagaaga atattcgtat ctttttcaat atcatagtta tcgtctgtgt
    15901 atgcagtcat tacttttatg aatggtgcag ggccaaggat atattcgact gtctctttca
    15961 ttcctttcgc taaatttttg tggcttgcaa gaataattgt gtacataatc cacctccatt
    16021 tatttataaa taatatacca ttggtaatct aaatgtcaat aaattatttc gtattaagaa
    16081 taaataaaat acctttggta attagtgggg cgaatctaaa aactttaact aaaattctct
    16141 tttgtaaatt tttttgaacg cggctatcgt ggtgattttt tatttctgtt tgtttaatga
    16201 atttgtaaat taggaggtga tttttgtgat ggtgtaaaca aagcctataa aaatgaagta
    16261 aagcattgtg ttttgtccag atgtcaaatc ttttatgtat cggttgtatg tatagtatgt
    16321 tacacccaaa atagataacc caagtccaaa tggagagaca gatgttcttt atattactgg
    16381 cgaagacatg aagtattctt ataaaatatc taaaaatgag tataagttta aagaaaataa
    16441 tttatctata taaactattc tgctttgatt agcaagtctt tcaatcggta aaatattatt
    16501 tgaatagatg ggatatggtt tattattttc taaatgtcaa attaagttag acaattgctc
    16561 ctcacttatg taactcaaaa atacttctaa cgatgttcga taatttaaag atttccttgg
    16621 tatatgattt cttttggagg caaccgccgc cacaaatttt tggtcaacct gattgaaatc
    16681 cattgtatta ggaagtccgt ctttacgtaa caatccatta gaatgttcat ttaatccccg
    16741 ttgtgacagc gttccaggat ctgcaaaata aatagaaata tcgttagcat tactcatgtt
    16801 tttccaattc gaaaattctt ttccgcaatc aaaggtaatg gatttgaaca agtttcgggg
    16861 aagcttttgt agccagtcag ttgtcacttt ttcaatatct agtgctttcc gtccttcggg
    16921 ttttaaagta ataatcactt ttgacaagcg ctcaaccagc gtgatgacag cacttttatg
    16981 atggacaccc acaatagtgt cgccttccaa atgtccaaat tcctgttgaa attcaggata
    17041 gtccgtgaga cgttctgaaa tattccgttt gaaggcttgt ttccctcgac gttctttatg
    17101 cccgttgggt ttccgttttc ctttcatggg tagtttcgag caatcaaatt agtttgtttt
    17161 aaacttgcga taaagggttc gaacagaaca tgaaattgaa aactcagcac gaccgataat
    17221 cacatcgggt gtccatcctt gcgccacctt gtttcggata tacgcatctt gttctgctgg
    17281 caaacaaatg ggtcgtcggc cgcaccgtgt tttatttttt tgatattgtt ggtaatagtc
    17341 atggatagat tggccttgtt tcaggaagtt gtacacattg tagatggtct gtctggaacg
    17401 atttgaggtc ttagcgacta cggtaacaga ttgatgcata ttgaaatagg cttctatcat
    17461 cacgagttca tttgttgtaa gatgggtata ggtcatttgt cttcactcct tataattctt
    17521 tggtcggaac tattttgagt gtagcacaaa tgactttttt agttgtctag cttaatttta
    17581 caatctgcgt ttaataaact ctcagtggga tagtttgact ccgtatatga ctccggttga
    17641 tttgattgaa ttcagatgat tatacatact ataaatttct ctattatgtt gccatttgat
    17701 ttgaataatg ctgaaagaag gattttagtc tataacgaac aagggaataa aaccaataaa
    17761 aaaagatata gaatatcaat ataacaaaaa gagattgtga cataattctc tgaaaaaagc
    17821 tctagaaatg acgagtcatt cgtcatttct ggagcttttt aggtaaaata aaagagtacg
    17881 agaggatact cgcactctaa atcattcaac ccttgaaagg atcaaaatca tgcatacaaa
    17941 ttataacatg aatcaactca ctttagatat cacaacttct tattccaaaa gaagatagca
    18001 ccgctgggtt tgtcaatgaa ctcgttgaaa cccttgaaat caacgagccg tatctttttg
    18061 gccgccctag gaattgtgat ttgagtgcca tgttaaaatt ggttcttttc gcatattgaa
    18121 aatgattgaa gaggactatc ggtattacaa aagacaatat aaccagcagg tgaaaaaatg
    18181 gagaaggtaa ttccgaaaaa agcttctttt ggcgattggg aataattgtt ggtaatggag
    18241 gaataaaatg aatgtttatg cagacgtttg atgtctacat caacatccat tcttaatact
    18301 cgaaggttcg ttgttgaaaa aactaacttt tcttcaacag aggatttacg tgcaaatcaa
    18361 gccgagtatt tcttcaataa tctttgtttg acgaaactgt ataaggcagt aaagaaagca
    18421 agtcctaatg ttaagtagcc caacggaaca caaaagaacg ctggttcaat cagcattccg
    18481 cttgaatcga ggtaagaacc gactaggatg gaagttgaaa ataacaaaat actcaagaaa
    18541 agaaatatac aaggaataat gaattttttc atggtgtctc cttctttgtt tttctagatt
    18601 atatagaata gaacctagga aaagataagt gtgacaaaat tgataggtgt gaaaggcatt
    18661 gagatatcaa ccagctttat cctttggagt atgctattgt gattaatgag acagaggatg
    18721 tgggaccaga cgatactgaa tcaacggaaa ttgttctaaa ccaccatcaa atagtagttt
    18781 ttctagtatt tgaaggtgat ttttttatgt tcgaaagtaa ctgtaaaaaa gataaataat
    18841 cggcagttat tctttcacat aagtaggttg gatgcaggtg agagaagttt attgtatgag
    18901 aggaagattg gtaatctaac tatagtttta taactaatat cagtttaagc tcgattattt
    18961 ttgtttcaaa gtaaaattgg aacagaaata tagaatagac atattgtagc cgatagttta
    19021 ttatgcttct agacacgttg aactggaggg
//
LOCUS       DACDIH010000049        18860 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDIH010000049 DACDIH010000000
VERSION     DACDIH010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.049"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            95..1780
                     /locus_tag="IV281_001854"
     CDS             95..1780
                     /locus_tag="IV281_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP8780837.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            1794..3077
                     /locus_tag="IV281_001855"
     CDS             1794..3077
                     /locus_tag="IV281_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8780838.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            3147..4016
                     /locus_tag="IV281_001856"
     CDS             3147..4016
                     /locus_tag="IV281_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8780839.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            4013..4849
                     /locus_tag="IV281_001857"
     CDS             4013..4849
                     /locus_tag="IV281_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8780840.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            4875..5942
                     /locus_tag="IV281_001858"
     CDS             4875..5942
                     /locus_tag="IV281_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP8780841.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            5939..6766
                     /locus_tag="IV281_001859"
     CDS             5939..6766
                     /locus_tag="IV281_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8780842.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            6811..7836
                     /locus_tag="IV281_001860"
     CDS             6811..7836
                     /locus_tag="IV281_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8780843.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            7833..10016
                     /locus_tag="IV281_001861"
     CDS             7833..10016
                     /locus_tag="IV281_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8780844.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            10016..11146
                     /locus_tag="IV281_001862"
     CDS             10016..11146
                     /locus_tag="IV281_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8780845.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            11143..11820
                     /gene="pgmB"
                     /locus_tag="IV281_001863"
     CDS             11143..11820
                     /gene="pgmB"
                     /locus_tag="IV281_001863"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8780846.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            11904..12431
                     /gene="msrA"
                     /locus_tag="IV281_001864"
     CDS             11904..12431
                     /gene="msrA"
                     /locus_tag="IV281_001864"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8780847.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(12632..13318)
                     /locus_tag="IV281_001865"
     CDS             complement(12632..13318)
                     /locus_tag="IV281_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP8780848.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            13398..14936
                     /locus_tag="IV281_001866"
     CDS             13398..14936
                     /locus_tag="IV281_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP8780849.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLSGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            15056..15523
                     /locus_tag="IV281_001867"
     CDS             15056..15523
                     /locus_tag="IV281_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP8780850.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            16042..16485
                     /gene="rplM"
                     /locus_tag="IV281_001868"
     CDS             16042..16485
                     /gene="rplM"
                     /locus_tag="IV281_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP8780851.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            16501..16893
                     /gene="rpsI"
                     /locus_tag="IV281_001869"
     CDS             16501..16893
                     /gene="rpsI"
                     /locus_tag="IV281_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8780852.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(16982..18208)
                     /locus_tag="IV281_001870"
     CDS             complement(16982..18208)
                     /locus_tag="IV281_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8780853.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(18280..18438)
                     /locus_tag="IV281_001871"
     CDS             complement(18280..18438)
                     /locus_tag="IV281_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8780854.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
BASE COUNT         6114 a         3443 c         3981 g         5322 t
ORIGIN      
        1 tcaaaattat ttaaaaaaat gctattgaca gtaagcgttt tccataatat gatcaaatcg
       61 ttgggaacgc tcccaaaaaa gaaaggggaa tccgatgtat acaaaacttt tacaagcgat
      121 ttatcgcgac tcagatttgt ctgagaaaac cgcctctatg attgaacaaa aagtcagtgt
      181 ttatcagcag aagaaaggcc aggcaggcaa actgcattgg gacgaagaag atattttctt
      241 gattacttat ggggatcaat tttttgaaga gcagcaacca actttgcgta catttaagaa
      301 attttatcaa aagtatttgg cagatacatt tagagttgta catttcttgc catttttccc
      361 atatacatct gatgatggtt tctccgttgt aaattatcag gcaatcaacg accgtttggg
      421 cacatgggaa gatatagaag aaatgagaag cgaaacacgg ttaatgttcg actttgtctg
      481 caatcatatg tctgccgaaa gtacttggtt tcaggaatat ttaaacttga atgatacgta
      541 caaagatttt tttattgatt tgccactgga tacagataca agcagcgtga ctagaccaag
      601 gaccagcccg ttacttacaa cgttcattga caaaaacggg aaagaaaaac acgtatggac
      661 gacttttagc gaagatcaag tagacttgaa ttttaaaaat ccagaagttt tattgaaaat
      721 gatcgatatt cttttgtttt acttagaaaa aggcgcttca tttattcgct tggacgctgt
      781 gggattttta tggaaagaac taggaacttc ttgtatacac ctgccagaaa cgcataaaat
      841 catccaatta ttccgtgcaa tcgctgatga agcagctcca ggaacggtat tgatcactga
      901 gacaaatgtg ccacacaaag ataatatcag ttattttggg aatggcgaga atgaagcaca
      961 gatggtctat cagttttcgc taccaccttt gacccttcaa gcgattcgtt cgggtaatac
     1021 aagtaagttg aaagaatggt taaaaaccat cgatttcgca tcgaaaaaaa caactttttt
     1081 taatttttta gcctcccatg acggtatcgg attgaatccg gtacgaggta ttctgccgga
     1141 aaaaacgatc cttgaattag tggaacagct gaagaaagag ggtacccgag taaactacaa
     1201 gaaaaaccca gatggaacga ccagtccata tgagataaac gctacgtatc tagatgcgtt
     1261 aagttctcca acagatacga cggaatggag aagcaaacgc tttttagctg ctcattcatt
     1321 tttggtaacg ttaccaggcg tgccggcaat ttatattcaa agtgtgctgg gaagtcgaaa
     1381 tgattacaaa ggcgtagaaa aaacaggaga gaatcgaaaa atcaatcggc aaaaattttt
     1441 gttagcagat attgagaaag agttgcagac agatccatta agaacagcag tatatggtga
     1501 attgacccag atgctatcta ttcgaaaaaa agaaaaagcc ttccatccga atagtttgat
     1561 ttacttactg gactcagcag attcttttat cagttttatt cgcgaagcga aggaagagaa
     1621 agtcttagtt atccataatt tttccaatca atcagcaggt tatcttttac caaaaggaga
     1681 ttacctagac ctgttttctg gacaaattat ccattgcagt caagaacgaa agttgatatt
     1741 ccagccgtat gaatatatgt ggctgaaaat aataaaataa gaggtggaaa aaaatgaaaa
     1801 aaattattgt tactacctgt ctgctagcta ctggatttct tcttactgct tgtggtgaaa
     1861 gtcaaagctc taacgaatca caacagacaa cgattcaatt catgcactct tctgtcgaac
     1921 aggaacgatt aagtgtgatc aatgaactgg ttgctgattt tgaaaaagaa aatccagaca
     1981 tcaaaatcaa gcaagtgccg gtagaagaat cagcgttcaa tacaaaaatc gttacgttag
     2041 ctcgttccgg taaattacca gaagtaatgg aagtcagtca ggattttgct aaagtgatgg
     2101 ataaagatga actgattgat cagacggcag tccaaaacgt tatcgaggaa gctggagaag
     2161 ataattatta tgacggagcc aaaaaccttg ttcgttcaga agatggaaaa agctatattg
     2221 ctgcaccaat tagcggctgg gttcaaggta tttggtatga caaagaaaaa ttatcagaag
     2281 ctggtttttc agaaccagaa aattgggatg atattttaaa aattgctcaa catttcacag
     2341 ataaagagaa taaacaatat ggaattgcta tgcctactgc tgaaggaaca atgagtgagc
     2401 aagcgttctc tcaatttgct ttgtcaaatg gagcaaatgt tttggatgat gaaggcgagg
     2461 taacgatcga tacggaaaag atgagagaag cattgtcgtt ttatcaaaac ttatcccaat
     2521 acacgatgcc aggctctaat gatgtgacag aaatcaaaga tgctttcatg aatggaacag
     2581 caccaatggc aatctattca acttacattt tgccttctgt ttatgaagcg gggaactcag
     2641 aaaatatcgg ttttgctatc ccaacacaaa aagatcaagc tgtctatggc acagtatctg
     2701 gattgacgat ttcttctggt ctagacgaga aacaaaaaga ggctgctgaa gcattcacag
     2761 ctttcttatc tcaaccgaaa aacatggaaa agtgggtctt gatgtctcct ggtggtgctc
     2821 aaccagtgaa taaacaagta gtcgaactgg atggttacaa agaaaatgaa gtcatcaagt
     2881 cttttggcga gctgccatct gagattgcta gtgcttttga tgaagtacaa gtctttggac
     2941 tagtcgggga aaagaatttc acgaaaatgg gagatatcac aagctcaggt gtgatcggaa
     3001 aagcagtgaa ccaagtaact gtcggaaacg aagatgtcga tcaagcttta gctgacgcac
     3061 aaaaaaatac gaaataacaa gaagttctgc caatggtgtt cgaaaaaagt agatagctat
     3121 tggcagatta tcagggggag gtttcaatga atcgtccaag gaaacattct gacttaaaat
     3181 tagccatggg acttttagca ccaagcatgc ttttattagc tgttttagtg ctgattccaa
     3241 tgctttccaa tattcagatc agttttttcg atatgccgct gaatcctaac tcagacgccg
     3301 tttttatcgg attaagcaat tatcttgaaa ttttaagtga tagtgaattt tatcgttctt
     3361 taggtttaac agttgcttac acattcttag ttgtagcagg cagtacagct ttaggattag
     3421 ccgtagctat tttgcttaac cgcaagttca gatttaggaa aacggtgcgt tcactgattt
     3481 tattaagtta tgttacgcca tctatttctt tgatttttgc ttggaaatat atgttcaata
     3541 atagttatgg aatcgtcaat gatctgttag tagataggtt gcagcttttc aacgaagcac
     3601 cgctgtggtt cgaccagcca gttgctagct tctttatggt cgttttgttt gctatctgga
     3661 gatactatcc gtatgcgttt atttcatttt tggctatttt gcaaacagtc gacaaaacgc
     3721 tttatgaagc agctgaaatg gacggtgcaa atgcttggca gaagttcaag atcgtgacac
     3781 tgccggcaat caaaccagta ctagcaactg tgattgtttt acgatctatc tggatgttct
     3841 atatgtttac agatgtctat ctactgacga ataaagtcaa tatcttaggc gtttatttgt
     3901 atcagacagc ctttgcgttc aatgatcttg gaaaagcagc agctatatcg attctattgt
     3961 tcatcattat tgcagcggtc gtcttgctct cacgaaagaa ggtggacttg aaatgaaaaa
     4021 taaaaagagc aggaaaatct tattttacgc gacgattttg ttgctattag ctgtcaattt
     4081 gtttccattt ttgatcatgc tgatgacatc attcaaggat tcacaagaag caattgcaac
     4141 gacacaaact atttttccga aagaatggac ttttgaacat tatcaggata ttttcaatgc
     4201 tgagattttt ccattcatca tgtatttcaa aaatagttta gtgatctcct tgatagcctc
     4261 cgtaactgca gtagtgattg ggatcagcgg ggcatacgct ttgtcaaaac tacgcttcaa
     4321 agctcgctcg aaaatcaatg ctagctttta caccgtttat atgttttccg gtattttgat
     4381 cgttgttcca ttatttaaaa tcatttccgg tctaggtttg tatgatacca atacagcact
     4441 gattatcacg atggtcgttc aaacacttcc tactgcgata ttcatgctaa aaagttattt
     4501 tgacacgatt ccgactgata ttgaagaagc agcaatgatc gatggtctta gccggatcca
     4561 gatcattatt cggatcatta taccattggc tatttctgga atcatttctg tcttcgttta
     4621 ttgctttatg gtttcgtgga atgattattt atttgcttcg attttcctgt cgacctccga
     4681 aaaatttact ttaccaatcg gtttgaacac attattcagt acaccagact acatttgggg
     4741 acgaatgatg gccgcttcat tggtgaccgc tttaccagtc atcattatgt atgcgttatc
     4801 tgaacgattt attaaaggtg atttagtcga aggcggcgta aaaggataaa aagaaaagaa
     4861 agaggaaaga aaagatgaaa aaattagttg cattagaacc aagaatagcc ggattcaaag
     4921 attacgaaga ccgtgagatc gaaaagaacg aagtacgtat ccgagtaatt ttcgctactc
     4981 caaaaccagg aacagaaatt gtcgacttta gaggattaag tccatttatc gatgaagaat
     5041 tttcagaaga atggcgccta ttcttgccaa gagaagaagg aagccaacgc ggtgtggtat
     5101 ttggcgaatt tcaattagga aatatgattg tcggtacgat cgaagaaacg ggcagtttgg
     5161 tgacagagta tcaaaaagga gagttagtct gcacttatgg acccattcgt gaaacgctga
     5221 ttacaaacgg tgttgataat tataaactac gtaaacttga tcggttggat caatggaaaa
     5281 atgcagtatg ttacgatcct gctcaattcg ctttaggtgg tatccgggaa gcacatgttc
     5341 gagcaggtga tgctgtagca atcttcggtt taggcgctat cgggcaaatt gcgattcaat
     5401 tagctaaaaa agcaggtgct actaccatcg ttgggatcga tcccatagcg cagcgtagag
     5461 aattagcaga aaaatacggt gccgattaca cgctagatcc tatgaaagca gatgtcggtt
     5521 ttcgtctaaa ggaattgaca gataaacgtg gagtagattc agttattgaa acgagtggca
     5581 gtgctgccgc attacaagct tctttaagag ggatcggcta tggcgggatc atttcttatg
     5641 ttgcctttgc taaacctttt ccagctgggt ttaatcttgg tcgcgaagca catttcaata
     5701 atgcaaaaat cgtctttgct cgagcatcta gtgaaccaaa cccagattac ccaagatgga
     5761 accgtaaacg aatcgaggac acatgttggg aattattgat gaacggttac ttggattgtg
     5821 aagaaatcat tgatccagtt gtgccatttg cagaaagcgc agaagcttac atgcgttatg
     5881 tagaccagca cccagaagaa agtatcaaaa tgggcgttca agtccgagga ggagaacaat
     5941 gaaattcggt acgcaagatc aagcattttt cccaagagac attttagaaa aatttagatt
     6001 tatcaaagac tgtggatttg aagaatacga aatcgacggt aagttgttaa tagaaaatat
     6061 cgacacagtc aaacaggcaa tcaaagaaac agggatccca gtagtgacag cttgtaacgg
     6121 ctacgatggg tggatcggtg actttatcga agaacggcgt cttaatggtg tacgtcaaat
     6181 tgctgagatt ttgcgtgcac taagcgaggt tggaggcaaa ggaatcgtta tcccagcagc
     6241 ttggggaatg ttcacttatc gattaccgcc gatggtttca ccccgaagca aagaaggaga
     6301 ctataaagca gtcagcgaat cacttcgtta tttagataaa gtagccgaag aaacaggtac
     6361 ggttatttat ttggagcctt tgaaccgata ccaagaccat atgatcaatt tgttagcgga
     6421 tgcgcgtcat tacattgact ctaatcaatt gaaacatgtg cagatcatcg ctgattttta
     6481 tcatatgaat attgaagaag atgatatttc aaaagcattg actacgcaaa aagagtatgt
     6541 gggtcatatc catttagcag ataatcatcg ctaccaacca ggatctggcg caattgattt
     6601 tactcgccat tttcaaacgc tacttcaaaa tgagtatgaa ggttatatgg tatatgaatg
     6661 tcgtttgcga ggagaaaatg aagtgaaaat ttatcaggaa tcattgcaat acttgaaaaa
     6721 ctgtctggaa acagcgcgtc atttaaatga tttagagaaa gaatagagaa agaataacaa
     6781 taagtaaata agcaaaggag ggagaaataa atgaagaaag tagcaatcat aggcgctgga
     6841 caagtagcag aaaaagtaca tgctgcttat tatcaaaaaa gatcagatgt cttagaattg
     6901 gccgctgtgg ttgatccagt cagccagcga gcagaagact tttgccgacg tacaggattt
     6961 tctcatgcgt atacatcagt tacacaaatg ctgaatgaga tttctcccga tatcgtgagt
     7021 gtctgtacac ccaatcgctt tcacttcgaa catgtattgg ctgccctcga agctaatgct
     7081 tcagtgatgt gcgagaaacc accagctatg acagctgatc aagcaagaaa gatggcagag
     7141 acagccgata aaaacgggca gattttggct tatggattcc agcatcgttt ttctgacgaa
     7201 gcaacaatga tcaagaatca tcttgatgtt ctcggggata tttattacgt agaagcgaat
     7261 gcgcttcgtc ggtctggtgt tccaggctgg ggaacgttta tcgacaaaga acttcaagga
     7321 ggcgggcctt tgatcgatta tggcattcac atgcttgata cagcgatgta tattactgga
     7381 tttcctaaag taaaaagcgt tcgggcgtac agcttccaaa aaattggtac gaaaaaaaat
     7441 tcagggaaat tcgggaaatg ggatccaagc aaattcacag tagaagatgc aatttttggc
     7501 acactggaat tagaaaacgg caagatcatc caactgaata cttcgtttgc tttgaatatc
     7561 agagaagaca aactgaatgt ggctttttgt ggcgatcagg caggtgctaa tctgtatcca
     7621 ggcgaactat tcgaagatgt agatggaaag ctggtttcct tacaaaagac cacgccagtt
     7681 aatgacccac atgaactaag tatggctcat tttgtgaatc gggcgctcgg tgaccagact
     7741 cagatgattg cagatggctg gcaaggatat gtcatccaga atctggtgga aaatttatat
     7801 cgttcggcag aaacaggaga agttattacc ttatgaaagt agaagaaaaa cagtttgatt
     7861 tagagtatat caataagtat gcgagtcaga tgaccattgg aaatggattt tttggtatca
     7921 gaggaagcca ggaggaagac tatcggaatc aagtaagggg aatgtttgca gctggtgtat
     7981 ataaccgacc tttaggagca gaatcagccg agttagtgaa tctcccagat gtcatcagat
     8041 gggaaataaa aatcgacggt gaacattttt ctcttgagac gatgaaagtt cttgcatacg
     8101 agcgggcact agatttagaa atgggagagc ttaaacgcta tgcgctcatt gaaactgctc
     8161 aaggaaaaaa agtggaagtc attacttgtc gtgttgctga tcaaaataat ctccatcagg
     8221 cagccctgaa agtggtcatc aagccgttga ccgaagactg ccaagtagat gtgaaaacgg
     8281 gtatcgatgg acagcaaacc aattttggta cacagcagtt ggttgaaaaa gaactgcgag
     8341 tatttggtga cgatttgatc agtgcatcct accagacaac agaaagtaaa attgatattg
     8401 ggattgcagc taaattcaat aaagcaggtg tattccaagc aaaaaatcgc cggatcaccg
     8461 cttccttttc agaaaagatc agaaaaaata cgtcctttac gctggagaag atcatcgtgc
     8521 tctctacctc tcttcagcaa acagacccgt ttaagaaaag tattgcccaa gcccagaaaa
     8581 gcgttagtta tacagaaatt ttagagcagt cgatgtctta ttggacagat ttttggcatc
     8641 agcatcggat aacgatccaa ggcgattctt ctttcgatca gttagcgatg gacttcgctg
     8701 cttaccattt acagattatg actccccgca atgatccaag tgttagtgta ggggcgaagg
     8761 gactaactgg cgaaggttat aaagggcatg ttttctggga tacggaaatt tttatcctgc
     8821 ctttcttttt atatcagttt cctgaagtag caaagaattt gctgctgtac cgttatgatc
     8881 gattagaagg cgcacggcaa aaagcaagag attatcacta tcaaggtgca cttttcccat
     8941 gggaaagcgc acacactggt caggaagaga cacccaaatt tgctgcattg aatatcaaaa
     9001 ccggaacaag acaagaagtt gcttcagctg ccgcagaaca tcatatcagt gcagaccttg
     9061 cttatgccgt tcaagagtat gtccaagcaa cagaggatca atcgttttta gaaacaaagg
     9121 gaaaaacgct cattcatgaa acagcagctt tttggctgag cagagctgta aaagtcgatg
     9181 atcatttaga gatacacaga gtcattggtc cagatgaata cacagaatat atcgacaata
     9241 atgcatatac gaactatctg gcgcattata atgtggcctt agctgttaaa tatcaagctg
     9301 gcgatccgct ttttttgaaa gaatgtcaaa attttttaga taagttatat ttgccagtgc
     9361 ctaacaaaga acagctgatt ccgcaagatg acacgtatct cagtaagcca gttattgatt
     9421 tgaccactta caaagaacgt cctggcagcc agtctatttt acttgattat gcccgtagcg
     9481 aagtaaatga actccaagtc agcaagcaag cagatcttgt catgctgtta tacttattac
     9541 ctgagtattt ttctcaacca atcgtttcta agaatttgca ttactatgaa aacaaaacaa
     9601 tccacgactc atcgttgagt aaagcgattc acgccataga ggcttgccgt atccaagaaa
     9661 tggaatgggc gtatcattta ttccaagaag cgtgcctgat tgatttagga ccagatccgc
     9721 attcatcaga tgacggaatt catgctgctt cattaggagc actatggcta gcagttatat
     9781 ttggtttttc aggtattaca gtaacgaatg gaactctttc tatttctcca agacttcctt
     9841 cagcatggga aaaaatttct tttcctttta tttggaaaaa tcacaggttg ctatttgttt
     9901 tgacaaaaaa acaaatagta ataaaaaaag agacgaaaga aacattacct atttttgtca
     9961 atggagacca ataccagctg gaaaaagaac tgatcttgga acggagggga aaataatgcg
    10021 tattctgatt gcgccagatt ctttcaaaga aagtctgaca gccttagagg cagcacaagc
    10081 cattcaatca ggttttcaag aagtttttgt tcaggctgaa tatgatctgc tacctatagg
    10141 cgatggagga gaaggaacgg tttcatcatt agcagacagt ttaaggttga agcggcaaac
    10201 agcagtcgtt actaatgcat ttggcaaaaa caaagaaatc cagtacgctt cagacggtca
    10261 aactgcgatt tttgaaatgg cagaaatcgt cggattgaaa gatgtcccac taaaccaaca
    10321 acagccactt aggttatcca cgcgaggagt aggagaactg atacgacaat tgttgcaaca
    10381 aggtgttcga cacatcatca tcggcgttgg cggaagtagt accaacgatg gcggcatcgg
    10441 catggcagaa ggtctaggtt atgcttttta cgatcaagat aatcaaaaag tagaagcact
    10501 gggagaaaat ctaggtaaaa ttgctacagt tgataaaacg aacgtgccta ttgagctgat
    10561 taaagccaaa atcactgtca ttactgatgt tactaatctt ttatgcggaa aagaaggcgc
    10621 ttcttttgtt ttcggaccac aaaaaggctt gaaagaaaat gaattagcag tggctgacca
    10681 agccatgcat gatttttata tgagatttgc ccctcaagta atcgaggcat cgggcagtag
    10741 tgctggtggt ggaatgggtg caggactgtt agcatttgct tcagggaaaa tagtctctgg
    10801 catcgactac gtattggatt tactggagtt cgataaacgg gtacagcaag cagacctcgt
    10861 aatcgtaggc gaaggacgtc tagatggaca aagtctaaaa ggaaaagcgc cagttgggat
    10921 tgctagaaga gtaccagagg gcgttcctgt gatagctatt tgcggaagtg ttggtaaagg
    10981 aagcgaaaaa gcagcagatg ttggcatttc ggccgttttt ccgatccttc ctgcagctgt
    11041 gcctttacaa gaagctttac agcaagccac tgataatctt catcgcacag ctaaaaacat
    11101 cgcatcttta cttcaagtcg ttcattttca aggaggtaat cgatgaaaat caaagcagca
    11161 ctattcgatt tagatggcgt tcttgtagac acagctcgtt atcattacga agcgtggctg
    11221 gttttagcta accagctgtc cattccattc actgagaaag aaaacgagca actgaaagga
    11281 atatccagaa ccgagtcact agagcgattg ctgtcatttg gaaaaatgga acagaaattc
    11341 tcagaaaaag agaaatcagc ctttgctgag caaaaaaaca atctatatct tcaagcgatt
    11401 caaaaaatgg atgaaacatc cgttttgcct ggtgcaatag ctgttcttga gtacttgaaa
    11461 aaaacaaaca tcaagatcgg attgggatca gcaagcaaaa atgcacgcct cattttagaa
    11521 aaaacgaatt tgacaagtta ttttgatgtc cttattgacg gcacgcaagt atcaaaagca
    11581 aaacctgatc cagaagtttt tctcaaagga gcacagcaat tgaatgtacc gccaaatgca
    11641 tgcttggtca ttgaagattc agaagctggt tgtcaagcag ctttggctgg taacatgcat
    11701 gtattgggaa taggtgaaaa cataaatctt ccttctgctg aatatgtgat tcctgattta
    11761 accgtttttg atcaagtacg ttcgttttgg catttatcag aagccgtatc acgtatataa
    11821 tagtttccgt tttttctgtc acaagcaaaa cgttatcctc cttttggaaa tcaaggtatg
    11881 atgagaaaaa agggggattt tatatgacaa aaaaagcagt tttcgcaggc ggatgctttt
    11941 ggtgtatggt caaaccattt gaagagcagc cagggatcat ttctgtgaca tccggttata
    12001 ctggcgggca tgtaccaaat ccaacttatg aacaagtaac aacaggcaca acagggcata
    12061 cagaagcagt agagatcgaa tatgatccag aagttatttc ttatgatcag ttggtggaaa
    12121 tctattggca gcaaaccgat ccgactgacg cattaggaca gttcgctgat cgcggtgatt
    12181 cttatcgtcc agtgatctac tataatgatg aagaagaacg aaatattgca gagcgttcaa
    12241 aacaagcatt gcaagacagc gggcgattcg ataagccaat cgtgacaaaa atcgaaccaa
    12301 gaagcgaatt ttatcctgca gaagattacc atcaggatta ttacaagaaa aacaagctcc
    12361 attacaatct ttatcgagaa ggttctggac gtgcaggttt ccttcgtcga cattggggcg
    12421 tcaagaaata agaagccgaa aaagctactt atggaacacc gtatatcaga ggttgtaaaa
    12481 ccagcatttt gagctaatac tgagggtcag ctgattgaac accatttagt ggagaggttg
    12541 tgaacctgtc gcctagctcc aagtcacaag gatttggtaa gagaacacta tatatcagag
    12601 gttgtgacaa gtcgtttgtc acaacctttt ttcaatacct aagatgaagg acaatcagca
    12661 acgcactgct caaaaaagga atgaaaggca gcttttccaa ctttttttta tgacagataa
    12721 agtaaacaga aaaaacggat attccaaaca gactagcaag aaacaagatc tgaagcaact
    12781 cttctagaga caagccacca ctccaagaaa gcatcaacag agtatcgcca gctccgagat
    12841 aggattgacc atagcgaagg cacacaccgc ctacgataaa cgttaacagc acactttgcc
    12901 attggaagac gccggcctgc aaccaaaaaa tccataaaat tgcccaagaa cagaatagcg
    12961 tgtgagaatg gaccatgaag taaaataaat ccatcaacga aaatagcaaa gccgaaagca
    13021 tccatatgaa cgtcgaaaaa tttcttgcgg aagaaaaagt aaagaaccag gcaaaaagac
    13081 caccacataa ggcttctgcc aacaaatagt agaagggcaa ccgacacctg caatagcggc
    13141 agcgaaatcg ttggacgatg atcgagagaa tcggaatcaa ttcataccaa gaaagcgaac
    13201 gacagcattt gacacaatga gatctaggat aaataataga gtgacctaac ggaatccgct
    13261 gggcggttaa acataaaaat gatccgatac agcaaccaat gataaaataa agtaacacgc
    13321 catctccccc ttttcaaaac caagatacgc aagcttaaac taaatttttg tacatacata
    13381 aaatttgagg tgaaaaaatg aacgtttcaa tcggattaga tatcggaacc acacaaacca
    13441 aagcagttgc tttcacagat gaagcccaag aagcagcatc cgcttatttt cgctaccctt
    13501 tgatccaaga aacacaaggg atggcagaac aagatcagga attgatcttt caaggtgtct
    13561 gcaccgttat aaaagaagta attaaacagt tgccttcaga cacaatcatt caatttgtct
    13621 ccttctcctc tgcgatgcat ggcctgatat tgttggatga agagaaaaca ccgttatctc
    13681 gaatgatcac ttgggcagat aaccgagcag cggacgaagc agaaaaactg aaatataccg
    13741 tgcttggcaa agaattttat cggcagactg ggacgccgat tcatccaatg acaccattga
    13801 acaaaatcaa gtggctgcaa aacagccaac ctgaattatt caaaaaaact gcttattttg
    13861 tcggtatcaa agactatatc ttttatagat tgtctggcga actgatcagc gactacggaa
    13921 cagcttcagg aactggatat tttgatatcc atgaattcca atgggcagaa gaagtgcttg
    13981 actacttgaa cattcgaata gagcaattgc cgcaagtctt tccttccacc tatcaagtaa
    14041 caggtctacc atcaaaaaca gcagcagaac taggtttgcc aaatgatata tcatttgttt
    14101 tgggaggggc agacgggccg ctttctaatc tagggttagg tgcttttggt gatacgatcg
    14161 ccgctttgac cgtaggaacg agcggagcac ttcgttttat tactactcag cctcagttcc
    14221 atccagaaat ggagacgttt tgttatgttc tggatcagaa acattgggta gtgggaggag
    14281 cgactagcaa tggtgctggc atctttgatt gggcagggca tacattttta caggaagtcg
    14341 ttcaatctgc taaagaaaaa ggagaaaatc catatgatgc actgctttcg gctatcgctt
    14401 ttgtgccagc gggtgccaat ggtctgattt ttcatcccta tttacttggt gagcgagcac
    14461 cgctttggga cgcagatgcg tttggcagtt ttatcggttt acagcggcaa catacagaaa
    14521 aagaaatgat gaaggcgatt ctagaaggga tctgtctcaa cctttatcgg attttaaaag
    14581 gagtttgcca ggaaaatcag ctcacagaga tccgtgctac aggcgggttt gctcaatctc
    14641 cgatttttcg tcagatgatg gctgatgtac tgggttaccc gcttgttttc cctactgtca
    14701 cagaagcatc cgcattagga gctgtgattt taggctggag aagcatcgga aagatccaaa
    14761 gtatcagtga agcacaggaa ttgatagaat tgaaagagca agttggagta caagaaactg
    14821 atcataaaaa atatcagaaa atctatccat tatttgtttc gacacaaaag catttagcac
    14881 agacttatca gcaatttgct caactgcgag aagaattagc tgattctgaa aaatgattgg
    14941 ctttacggat aaaaaagcga actgaaaatc gttctcaggt aaatgatttt gttgtacttt
    15001 tcattgaaag tctactatta ttttaagtgt aagaaaatgt taggaggcaa gaaagatgaa
    15061 attccaacaa accaaagaag tattaaatca attagttgca gatctaagcc aaatgtctgt
    15121 tgttgttcac caaacacatt ggtacatgag aggaccaggt ttccttacat tgcacccaat
    15181 gatggatgag ttcatggatg atttgaacgc tcagttagat gaagtatccg aacgcttgat
    15241 cacattagac ggttcacctt attctacatt gcgtgaattt gcagataaca caaaaatccc
    15301 tgatgaaatt ggccgctggg atcgtactat tccagaacgt ttggaaacac ttgttgcagg
    15361 ctatcgttat ctagcagatc tttaccaaaa aggaatcgaa gtttctggag aagaaggcga
    15421 cgatccaaca caagatatct ttatcgaatt caaaacagcc acagaaaaac gtatctggat
    15481 gatccaagct catttgggca aagcgccaga gatcgacgcg taaaaactgt tgttgaactt
    15541 gaccgaaaaa gaggttgtga aatcagcgtt ttgacctgag tattagagaa acaaacgtca
    15601 aaatagttcc tcatattttg ccgttttttg agctaatacc gagggtcagc tgattgaaca
    15661 ccgtttacca agaggttgta agcctgccgt tcagctccga gtcacaagaa acaattttaa
    15721 aaaacagctt cttatgtttt atggatattg tgacttgtga ccgaggagtt ggcaggagaa
    15781 caccgtttat tcaaaataag gggttgtgac aagacttttg tcacagcctc tttttgtttt
    15841 tcattttgac aggaaacgac ccttttatat aaaagaaaga aaagaagtaa agtttcatat
    15901 aaatttttca atcaagcaga aaaacgttga cattgactag attagcgggt aatatgttaa
    15961 atggtattgt ttgccccaca aagagccccg gaaactcgaa gtgtatgtca aacatcagaa
    16021 cttaaattgg aggaaaataa cgtgcgtaca acatatatgg ccaaaccagg cgaagtagaa
    16081 cgtaaatggt atgtagtaga cgcaactgat gttccattgg gacgtctttc aacagttgtt
    16141 gcatctgtac tacgtggtaa aaataaacca actttcacac ctcatttgga tactggcgat
    16201 ttcgtcatcg tcatcaatgc agacaaagtg aaattaacag gtaaaaaagc gactgacaaa
    16261 atttattacc gtcacagcaa ctacccagga ggattgaaat caatcactgc tggcgaattg
    16321 cgtgctaaaa attctcgtcg tttgatcgaa acttctgtaa aaggtatgct tcctaaaaac
    16381 actttaggcc gcaaacaatt tacaaaattg aacgtatacg gtggagcaga acatccacat
    16441 gcagcacaac aaccagaagt tttagacatc acaaacttaa tctaatagga gggaatttca
    16501 ttggcacaag ttcaatatat cggcacaggc cgtcgtaaaa acgcagttgc tcgcgtacgt
    16561 ctagtaccag gaactggcaa aattactgta aataaaaaag atgttgaaga atacattcca
    16621 catgctgact tgcgtgaagt aatcaatcaa cctttcgctg ttactgaaac aaaaggcgca
    16681 tatgacgtat ttgttaacgt aaacggcgga ggatacgctg gacaatcagg agctatccgt
    16741 catggtatcg ctcgtgcatt gctagaagtt gatcctgact tccgtttagc tcttaaacgt
    16801 gctggcttac ttactcgtga cgcacgtatg gtcgaacgta aaaaaccagg tcttaaaaaa
    16861 gctcgtaaag cttcacaatt ctcaaaacgt taatattctt tttatatcaa tatttcagga
    16921 cacttttcaa ttatttggaa agtgtctttt tttataataa gaacacattt gagaacacat
    16981 gttaatcaca cttattcaat cctttttgaa atgcttcaaa aacagattta tcaactacgt
    17041 caggtgtgtt cacgtatatt tttgtagtag aaatatccga gtgcgttaaa gcattagaga
    17101 tttgattcat atttgctcca ccttccctag ctatagttgc ataggtatga cgaagtttgt
    17161 gtggagataa ttgagccaat tcaggatgcc ttttttttat tgcattgatt cgatagttta
    17221 agtagtcagc gtgaacagga caatttactt ctccagaagg tttggaatag gtaaagaggt
    17281 actggtcagg tgtttgtata atttttagtt tcaacaattg ttcagcctga gctgatttcc
    17341 attctcttaa taatgtcatc acaacttctg gtgcctgaat aacggtgtct ttccaacctt
    17401 ttgtatgtgt ctttctttga tatttatcta aagcatattt gagacgaacc gtatttttat
    17461 taaaatcaat atatttccat tgcagagcat aagtctcact ttttctatca cctagatata
    17521 aggtaagcat gaaaagtgtg taatcgtgaa gtgtaagcaa gtgattattt aagtcattgt
    17581 ttactgcttc tatccaatca gtcagctctt ctgatgataa tgcttgattc ccttccctta
    17641 tccttttttc ttctaaagcc attttcttag gcgcggtaat acattgaata attttggtag
    17701 ttcggttgaa ttcgatataa catagtattt cggcaatatc aaacatagag cgaacatagc
    17761 ctttgataat ttttatattt gcgtattctt tcgatttctt tgttaattca tctgatatta
    17821 tctcagtatt tgaattcaga taattcattg catattcacc aaacattgga aggatgtgca
    17881 atcggaatat atccttagta ttactaattg ttacattact tggagccctg tttgacctag
    17941 tagtttgacc tagttcatat ctaggaagcc attttgattc ataaaaatct ttaaatttta
    18001 tttttccttt caattctagt gagtttgcat tctcctctct taaaactgtt tcaatcttct
    18061 ttttcatttc cctttcggcc ttaatagcta attgcctagt tggaaatact ttttgatact
    18121 tctctgatgc aattccagtg atttctctga atttttttgg atacctcaga ttagttttat
    18181 atttcccatt tgaaagcttt gtaggcaaac ttcctcactc ctttaaaaag taaggcagta
    18241 catttcgcta tttatagtat atgtgacgag aagaaaattt caattttttt tgaatgtatt
    18301 catgaaatct ttgatatcct ctacgtcaaa gcgaactacg ccatcaattt ttattactgg
    18361 aaaattatat acacgaatcc acttatctaa agtattatta tcaattccta gaaattcaca
    18421 ggcttgtttt ttattcaagt aggctttcga ttttataaaa tcatctccta aaataatagt
    18481 ttaaaatttt tgaaccgctt tgggtaagca cataggaatt tcacctctcc ccagtttacg
    18541 gcgagcctta cggaagtatc attatttact gctattgtca tggcattttg ttgtcccaga
    18601 acaaaagaaa tctctaagta gttgtcgcta actaggtcat ggcgtacttg tatttctagc
    18661 tcgaatagca gggaacgtta ttcaaaaatt atatctaatt atcaaagatc aatataggaa
    18721 tatcatccta atcactatta ataacaagaa gaagattaaa aaagaaacaa aaacggaaaa
    18781 aaatttatct tgattccgag aattcaatat agataatacg gtgctgaata aaattatctg
    18841 gttgttaaga taaattatca
//
LOCUS       DACDIH010000050        18759 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDIH010000050 DACDIH010000000
VERSION     DACDIH010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.050"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(325..2322)
                     /gene="tkt"
                     /locus_tag="IV281_001872"
     CDS             complement(325..2322)
                     /gene="tkt"
                     /locus_tag="IV281_001872"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP8780855.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPIGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2424..2675)
                     /locus_tag="IV281_001873"
     CDS             complement(2424..2675)
                     /locus_tag="IV281_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP8780856.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2822..3445
                     /gene="lexA"
                     /locus_tag="IV281_001874"
     CDS             2822..3445
                     /gene="lexA"
                     /locus_tag="IV281_001874"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP8780857.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3624..3842
                     /locus_tag="IV281_001875"
     CDS             3624..3842
                     /locus_tag="IV281_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780858.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4130..4642)
                     /locus_tag="IV281_001876"
     CDS             complement(4130..4642)
                     /locus_tag="IV281_001876"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP8780859.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4656..6953)
                     /gene="recJ"
                     /locus_tag="IV281_001877"
     CDS             complement(4656..6953)
                     /gene="recJ"
                     /locus_tag="IV281_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP8780860.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLMHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPVE
                     FLDLVAIGTIADLVSLTDENRILVSLGINAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSN
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     IMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7095..7529)
                     /locus_tag="IV281_001878"
     CDS             complement(7095..7529)
                     /locus_tag="IV281_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP8780861.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7539..8327)
                     /locus_tag="IV281_001879"
     CDS             complement(7539..8327)
                     /locus_tag="IV281_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8780862.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETDFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8324..9265)
                     /locus_tag="IV281_001880"
     CDS             complement(8324..9265)
                     /locus_tag="IV281_001880"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP8780863.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9442..10029)
                     /locus_tag="IV281_001881"
     CDS             complement(9442..10029)
                     /locus_tag="IV281_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP8780864.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10250..11563)
                     /gene="obgE"
                     /locus_tag="IV281_001882"
     CDS             complement(10250..11563)
                     /gene="obgE"
                     /locus_tag="IV281_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP8780865.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11851..12861
                     /gene="gap"
                     /locus_tag="IV281_001883"
     CDS             11851..12861
                     /gene="gap"
                     /locus_tag="IV281_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8780866.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12946..13881)
                     /locus_tag="IV281_001884"
     CDS             complement(12946..13881)
                     /locus_tag="IV281_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8780867.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            14067..15026
                     /locus_tag="IV281_001885"
     CDS             14067..15026
                     /locus_tag="IV281_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8780868.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYLHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(15112..16206)
                     /locus_tag="IV281_001886"
     CDS             complement(15112..16206)
                     /locus_tag="IV281_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8780869.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            16395..17156
                     /locus_tag="IV281_001887"
     CDS             16395..17156
                     /locus_tag="IV281_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8780870.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(17271..17711)
                     /gene="fabZ"
                     /locus_tag="IV281_001888"
     CDS             complement(17271..17711)
                     /gene="fabZ"
                     /locus_tag="IV281_001888"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8780871.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            17931..18686
                     /gene="fabI"
                     /locus_tag="IV281_001889"
     CDS             17931..18686
                     /gene="fabI"
                     /locus_tag="IV281_001889"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP8780872.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         5434 a         4047 c         3296 g         5982 t
ORIGIN      
        1 gagcaatcgc ttttttattg atcaccattt ttcttttagg tctatggtgt cgggcactgc
       61 gggtcttcag ctcttgcatt caaattcctc ctcaaaataa aaaaacttaa taaaaagttc
      121 tcttcttaaa acaaacttaa ttaattaaat tatactttta attctcaaga aacacaaact
      181 aatccgaaga attctttgtt ttgtcattta aatgtaacat agccgaaaac gaaaagtaat
      241 attgatttat tcttctaaca cataaagaac agctgtctcc aacttgcaga cagctgttct
      301 tatcagtcaa acaattgctt ggatctatga tagttgattg tacgtattca cgacattttc
      361 tactgtaaat ccaaattctt ctaaaatttt agtgccaggg gcagatgcac cgaaatgatc
      421 gattgtaatc gttgcgccct ctgttcctac gtagcgttcc caaccaaatg atgcagcagc
      481 ttcgatagcc acacgtttct tcactgattt aggaagtaca ctttctttat attcagaaga
      541 ttgtttttca aagaggtcaa agcttggcat agaaacaaca gaaacatctt tgcctttttc
      601 agccaattct ttttgagctt tcacagccaa atcaacttca gaacctgttg caatcaaaat
      661 accttctggt gtttctcctt gtgatggaga aagaacatac gcgccttttt tcaccatttc
      721 atctgctact tcttttgtag aaggcaatac tggaaggttt tgacgactca atacaaggat
      781 agtaggcgca tctgttgtct ccatcgctac tttccaagca gcacgtgttt catttccgtc
      841 tgccggacga atcacttgaa caccaggcat gcagcgaaca gatgctagtt gttcaatcgg
      901 ttcgtgcgtt ggtccgtctt ctccgacagc aaccgagtca tgtgttaaga cgtaaacaac
      961 aggagtatgt tggattgctg ctaaacgaac tgctggtctt agataatcga caaagacaaa
     1021 gaatgttcca ccgtagatac gagttccgcc atgaagttgg ataccattca tagccgaagc
     1081 cattgcaaat tcgcggacac cgaaccagat gttgcgtcct tcataatgtt caggagtgaa
     1141 atctttatct gcagccacca ttgtattatt ggaacctgac aagtccgctg aaccacccca
     1201 gaagcttggt atagctttcg ataattcttg gatgacttct ttacttgaaa cacggcttgc
     1261 ttgactgctt ccaacttcat aagttggcaa ttctgcatcc cagttttctg gtagtttgcc
     1321 atcaaaagca tcttcaaatt gttgtgctaa ttcaggataa gcttttttat aattggcaaa
     1381 catttcattc catgcatctt cagctttttg tccttcttct atcatcgttt gatggaagcg
     1441 ggcagctact tcttctggaa cagtgaaatc tggatactcc caaccataaa cttctttagc
     1501 catcttgatg ccatcttcac caataggagc accatgaaca gcagatgttc cttctttagg
     1561 agcaccatag ccgatgactg tttttacttc gatcaatgtt ggtttgtctg tttcagcttt
     1621 tgcttcttca atcgctttag agattgcttc taggtcattt ccgtctttca ctaagatatg
     1681 ttgccagcca tatgcttcat agcgagcacc aacgttttct gtgaaagctt ttgaagttgg
     1741 accgtctagt gaaatatcgt ttgaatcgta taaaacgatc aatttcccta gtttcatatg
     1801 acctgccatc gaacttgctt cttgagatac cccttccatc aagtctccgt caccgcatat
     1861 tgcatacgta tagtgatcca tcacattaaa gttttctttg ttatacgttg ctgcaaggtg
     1921 cgcttctgcc attgccatac cgacagccat tgcaatccct tgtcctaatg gccctgtagt
     1981 ggcttctaca ccatctgtgt gatgtacttc aggatgtcct ggtgttttag aatcccattg
     2041 acggaattgt ttcaaatcat cgattgtcac ttgatagcca gctaaatgaa gcaagctata
     2101 caacattgct gacccatgtc ctgccgaaag aacgaaacgg tcgcggtcag cccagttttt
     2161 tgatgtcgtt ggatttactt tcaggtgttt tgtccataac gcatatgcca taggtgctgc
     2221 gcccatcggc aatccaggat gtcctgagtt tgctttttgg actgcttcaa tacttaatgt
     2281 acgaattgtg ttaacgccaa gttggtcaat tttgtcaaac aaaataaccc ctcctattaa
     2341 tttaggtttt ttttaccttt atttcttaca ctttcattct ataagatgtc acacgaaaaa
     2401 gcaatctttt tcagtatttt ttactaaaag ttgtcatttc ggttgtgtaa gccttttttc
     2461 ttttggattt cttttaattt ttccggggtc acgtcattgc cctcaggatc gacgactttc
     2521 attccttcga tatgatgacg cataccggaa cgaaaagctt ccaaatactc tttgcgtaag
     2581 ttgatttgtt cttgttcttc ggcttttgag agaccatttt ccttcttttt acgtgcaagt
     2641 tcattgatcc tctctatttt ttcaggtgac agcatgctca gccctccttt tactctcctt
     2701 catattatat aaaaactgaa aaaaaaacaa ctagataaac taaataaaat acgaacattc
     2761 gtttgattta gcgcttttta tatggtacta tttatttatc aagtgaaaga aggtgtacct
     2821 tgtggcccga caaactgaat caagacaaat cgaagtctta cgatatatcc atgaacaagt
     2881 aacccaaaaa ggatacccgc cgactgtccg tgaaatcggg gaagctgttc agctttcttc
     2941 tacttctact gttcacggtc atcttgcacg gcttgaaaaa aaagggttta tccaaagaga
     3001 tccgacgaaa cctcgtgcaa tcgagttgac aaaacaaggt ttagaaaaga ttgggattcg
     3061 tccaacaact attccagtac ttggcgtcgt aacagctgga gaacccattt tagcagtaga
     3121 ggatgcttca gacttctttc caatccctcc taatctttct tccgaagaag gcagtctctt
     3181 tatgctgaca attcgtggag atagtatgat caatgctggg atcttggatg gtgacaacgt
     3241 gatcgttcgg aagcaagaaa ctgctcaaaa tggcgatatt gtcattgcaa tgactgccga
     3301 ggacgaagcg acttgcaaac gattctataa agaaaaaaac tttttccgtc tgcagcctga
     3361 aaacgacttg ctcgaaccaa tcatcttaga tcaagtatct atattaggac gagtagtagg
     3421 actttatcgt agtcatatct attaattgct actaataaaa gaaaactttt taaaaagagt
     3481 ttgagacgat ggtctcaaac tcttttttcg cccgggttct tcttctttca aatttttatc
     3541 acttccctct ttacaaaccg aacaaatatt cgtatactaa atatacgaac aattgttcgc
     3601 ataaattaac gaggtgagaa ttcatgaaat caatgagacg tcttggctta gtattaactg
     3661 ttttgttttt aggtattatt attggtaatt tcggcatacg tccagcttta cttgtgtttg
     3721 ttccgttatt tactttgtgg ttcatgttgt gggatgaaaa gaagtaccgt caatctgagc
     3781 ggcgcagaat tcatgaagat catttctatc atgagcccta ctacagatcc aggcaatcat
     3841 aataaggatt gactgctaat tattcaaaag acaagaagtt aaacttttac gggggtgtga
     3901 caaaaatcgg tcacatcctt ttttcgaata aacggtgttc taaagccagt ttttcggtat
     3961 taagtcaaat tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat
     4021 ttataaaaaa atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt
     4081 taaaatcgct tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca
     4141 ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga
     4201 tcaaaaatgc gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt
     4261 tgattgttcc tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa
     4321 tggcatcctt atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga
     4381 tggtttctcg aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat
     4441 aagcaacagg acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc
     4501 gtttttcttt tgcataattt acgatttgct tcgtagcttc ccgataagcg tcaccatctg
     4561 ccatcagtgg ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg
     4621 caatataatc ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg
     4681 gtaggaatat cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgccgctgg
     4741 tataatctgc tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg
     4801 ttctctattg taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt
     4861 tcagggatac gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct
     4921 tgagaatgga tcagtttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa
     4981 taggcctcat caggcgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa
     5041 acttctttca gatccgctaa atcatctgga caatccataa taactagaga gttcggtttt
     5101 cttttttcaa ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg
     5161 ataatctcag gcacgaattt caacgtagaa gaatcaaatg ctaaataaag gctaccttcc
     5221 gacgttcggc tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc
     5281 acagaaaaat cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa
     5341 agctttccta ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca
     5401 aaagcgaccg catcaagctg actcgatgca ttggaaagga caaatttcag atgctgttgg
     5461 tttgctccga ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa
     5521 ggattatccg taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact
     5581 tcatttggca gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga
     5641 tgatcgacta tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc
     5701 gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca
     5761 agcatttcaa aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct
     5821 tcttttaaag taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca
     5881 atgcccaaaa cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta
     5941 ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg
     6001 atctcattca atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac
     6061 gagacggcag gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa
     6121 aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc
     6181 tgaagtccta tccgttctga atgatgaatg gcattgattc ctaatgaaac aaggattcga
     6241 ttctcatcag tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat
     6301 tctacaggcg gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc
     6361 aaatctggaa atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct
     6421 ggtaaattct caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga
     6481 gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt
     6541 ccttcttcga tcttttcttg gtatacagat tggtttggtc catacccatg ctcaaatcga
     6601 tttggcagga atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt
     6661 gcactggtga tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt
     6721 acagcttctt ggattcggct gattgcttta tccatatcgt gcatcagata cggatcatgt
     6781 aattcttgtt cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt
     6841 tgccacagta acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag
     6901 gattcagaag gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca
     6961 ctcctaactt agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa
     7021 aaacagatca gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt
     7081 atgaaatatc cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt
     7141 tcttccggcg ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa
     7201 gcagcttcca actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct
     7261 tcaattttct tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt
     7321 tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc
     7381 agcgcagaac cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt
     7441 acaggaacat ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat
     7501 ccaagaatca cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg
     7561 ctggctaaat aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa
     7621 ataaacggtt gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc
     7681 aacttggcag gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca
     7741 gccttatcga agaaatctgt ctcgattgga cctggattca ctgtcgtaac agctacgcca
     7801 agtggtttca attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca
     7861 gaataaaccg ttgattttgc agttgccatt tttccagcca tagacgcgat attaatgata
     7921 tgaccttgtc ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct
     7981 aagatgttga cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg
     8041 aaaatcccaa aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc
     8101 ttttcatata attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc
     8161 tctttgccgc tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt
     8221 gcgcaactta taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga
     8281 ccgcctgaac tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct
     8341 cgcgaaatgg aatctcaatg ctatcaaaat ctttcatgat cgtcgtgttt tcaaacactt
     8401 cttgtgcttc tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag
     8461 taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg
     8521 aatggaaata gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca
     8581 gaatatcgct attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa
     8641 tagtcacgat tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc
     8701 catcttctag ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa
     8761 tacctaattc ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac
     8821 gataaccaaa actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat
     8881 cagtaaaaat cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg
     8941 tttgcgaaat acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct
     9001 ctaatggcgt atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa
     9061 tatgatcgcc gtgtaaatgt gtaataaaaa ttttaccgat ttttctcgga cgaatagtcg
     9121 ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct
     9181 cgtctaaaag tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac
     9241 ctgttcctaa aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt
     9301 ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa
     9361 cgttctaaag cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt
     9421 gagatcctct gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt
     9481 ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc
     9541 tttcgcatca tgcacatctt tttcgacatc ttcataatcc aagcgggtaa tttctccgcc
     9601 taattccatc attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat
     9661 cagtcctgta gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc
     9721 ttttatttct ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa
     9781 aatccctagc ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga
     9841 tttgttatta ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg
     9901 atcatggctg acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt
     9961 gatttctgaa aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg
    10021 tttgctcatt aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgta
    10081 aaataagaaa agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta
    10141 tcacagctcc tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct
    10201 cccttaaatc tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa
    10261 attcaaattc aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa
    10321 gagcttcatc cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat
    10381 gatcgaaatt cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg
    10441 catctgaatc acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag
    10501 tttcttcttc cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg
    10561 ctgtagcatt caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg
    10621 ggatgtcatc agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta
    10681 gattttccgg tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta
    10741 gcaagcgtaa gttataagta gataattctt tgttgatggc aagatagtct tcataaggat
    10801 ctcgtccttc catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat
    10861 gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca
    10921 agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttagggacga
    10981 gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag
    11041 tcgatttccc gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta
    11101 gttcgatttt tcgctcttgt cctggttcgc cgttttcagc aatttccggt gcaggattcc
    11161 gaggagaggc aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg
    11221 tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg
    11281 ttccttgtgg tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca
    11341 tcccattttc acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac
    11401 gtaacccttc atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg
    11461 gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc
    11521 cagctttcac atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt
    11581 attcatttat ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta
    11641 tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt
    11701 ttatcatttg gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa
    11761 tattctttgc gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac
    11821 agcaccccta ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt
    11881 gggcgtattg gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta
    11941 gtagcaatca acgacttaac aagtccaact atgttagcac aattattgca atttgactct
    12001 actcatggta cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc
    12061 gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac
    12121 ggtgtagata tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg
    12181 catttagatg ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc
    12241 atcgtctata acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt
    12301 tcatgtacaa caaactgttt agcacctatg gcttatttct taaataatga atttgggatc
    12361 gaagttggta caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga
    12421 cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat
    12481 tctactggtg ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa
    12541 ggacacgcac aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg
    12601 aaaacaaaag taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat
    12661 ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa
    12721 ggttcgatct ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt
    12781 aaaactgttg catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta
    12841 gaaaaattcg ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat
    12901 ccagtcaaag gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg
    12961 gcttaaaaat tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc
    13021 atgctcggct ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt
    13081 atctaacatt tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg
    13141 ctggtttttc ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac
    13201 ttttgtcact agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc
    13261 tagctgataa gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt
    13321 cacattatct ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac
    13381 accgtataga acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg
    13441 tacatagtct ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg
    13501 tcctcttgca tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt
    13561 ggtcatcgtt tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt
    13621 cgtttcagca ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca
    13681 atcgcttcgg ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg
    13741 tgttgtacca ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt
    13801 ataaaaataa ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag
    13861 gattttcatc caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg
    13921 aattagtata atcaaagaat ctctactatg caatgttcat ttttataacc cgcgaattgt
    13981 aaaattaagt tagaaaaata aaaaggcatt tatggtacac tcaaattgta tttccgacga
    14041 aagaaaacaa aggagtgtaa ccataaatga cctacaaaca tcttaccata gacgaactga
    14101 caatgataga atcatattat cttcaacata ataagccggt tgaaatcgct aaccgaatgg
    14161 gacgtgctat acaaactatt tataatgtag tcaataagtt caagcaaggc aagactgctc
    14221 ttgattattt gcaccagtat aaagaaaata agaaaaaatg tggtagaaaa gtcattcaat
    14281 tacctgctca tgaagtagat tacattaaag agaaagtcac tcttggttgg acgcctgacg
    14341 tcattatcgg gcgaaaagaa aggtctgttt catgcggtat gagaacactt tatcgtttat
    14401 tttctaaagg aatatttgat attgacacac taccgatgaa aggtaaaaga aaacccaatg
    14461 gccatcagga aaaacgggga aaacaacagt atcagcgctc aatccatgat cgacctgata
    14521 attatcctga tttcaattct gagtttggtc accttgaagg tgatacgatc gttggcattc
    14581 atcataaaag tgccgtcatt actttagttg aaagattatc taaagtcatt atcacgatta
    14641 aacccaacgg ccgtaaggca ttagatattg aaactgccct taatcaatgg ttttctcgct
    14701 tccctaaaaa cttctttaaa tctattacgt ttgactgtgg aaaagaattt tctaactgga
    14761 aagccattag taaccaacat gatattgata tatattttgc ggaccctgga acaccttctc
    14821 aacgcccatt aaacgagaat tctaacggga ttctgcgtcg taatggactg ccgaaatcaa
    14881 tggattttag aaaagtgaat cagacattta tttccagtgt cagcaatcaa cgtaatcata
    14941 ttccaagaaa atcattgaat tacagaacac caatcgaaat atttttgagc tatgtacaag
    15001 aagcatttta ttctagctta atttgacaaa tcataaccta taaaaaaaca aaataaaaaa
    15061 gcagaaataa ctggctattt ctagcggatt atttctgcaa taactaatta tctatttttt
    15121 attttcaatt aatttaaagc ttgaccatgg tttcaaaaag tcaaggagga ataattcgtc
    15181 atcggagatt cttcctacaa cattgacacg tccgtcgttt ttcatatcct tcaaggcgat
    15241 ttgtgtttct cctttgtatt gaccgtaccc ttcattgtca atcagtacat ccccatgatg
    15301 catatctcgc gtattatgag gcgggaaatc tttatctttg tagtatacac gagtcatcgt
    15361 tgaacgaagg atatattctg agcgatcccc tcgataacta tgaaggttat caaacaagca
    15421 gattcgttcg ttttctgtaa taccttcttc cgtatctact ttcaatgttg gataatccgc
    15481 gttaaatgct tctgccattt ctttcaattc ttcttcactt gcgtacgcat ttccaataga
    15541 aatatcatca atcaatcctg taaggattaa atgcttcact tgtgtcgcaa tttcaagatc
    15601 tcggtggtct tccagcgtgc acaagccatc ttgcgtaggc caagggccaa actcagcaga
    15661 ttgcgaattg acgaatgcca tcgtattcaa attatatttt ctgaattttt ctgaacaaaa
    15721 aacaaagtgt tcgtatccta aaccagagta tcgatgagga taaaaattat gagaacctaa
    15781 taaattatcc gtgtttggcg aataactcat gatgttgtca acgtaattcg tgcccgaact
    15841 catattgatc tcgattttga taccaaaagg attgcgcgtc atttttgctt cttctgctcc
    15901 tgtgaatccg atatctaagc ggaccccgta ggcacccatc ttatggaaaa aagataagtc
    15961 atcgtaagaa atctccaact gttcaaataa cgctggattg atatcaacca ttacttccat
    16021 gcctaggcta ttcgcgtaat caactacttc tttaaattct gaaagaactt tttctttatc
    16081 atcattgatc tgcagcaaac tggtaaaaac ccgtttatac ccatacttat gagctaaatc
    16141 caagtacgct ttgtcctttt caaatgttga ccgttctgga taaatcgaga ttcctaattt
    16201 tcccatatta ccatttcctc ctgcatcttt tcgtttttgt atccacttac attatagcaa
    16261 acacaaagta aatggtctac accttttctt tttcttttaa aataaatgat aaattccaat
    16321 aatttaagta ggtattttcc ctctattttg ttatcattaa agataacaaa atagtaaatt
    16381 ggaaaggaac agttatgaag aaaaataaag ttattttaag tattagttta gcagcagcat
    16441 tgctgtttgc tgctggttgt tctgcaaaca acacgacttc ttctagttcg tctacagaaa
    16501 gttcaaaaag tacatctact gcggaaagca cgactgccag tagcgtcgat ttgaacgcat
    16561 tggaattacc acaattgacg aaagatgtcg ccgaaaatga agatctggtc gaaatggtga
    16621 cgaataaagg cacaatcaaa atcaaattat ttcccgaaca cgcaccaaaa gcggttgaaa
    16681 actttatgac acatgcaaaa gacggttact acaacggact gactttccat cgcgtcatca
    16741 aagatttcat gatccaaggt ggcgatccta aaggcgatgg cactggaggg gaaagcattt
    16801 ggggagaagg atttgaaaca gagatcaaca accaccttta taatataaga ggtgcgttat
    16861 ctatggctcg ctcacaagat cctaacagta acggcagcca gttctttatc gttcaaaata
    16921 cagatgatat gcatgatggt cttttaaaag acgactatcc tcaagcaatc atcgacgctt
    16981 ataaaaacgg cggttatcct tcactagatg gccattatac tgtcttcggc caagtgattg
    17041 aaggaatgga tgtcgtggat gctattgcaa atatcgaaac agatagttca gacaaaccaa
    17101 aagaagaagt aaaaatcgat aaaatcaata tactgcaaga agcaaaacaa aaataaaaaa
    17161 ataacgtctg aatcctgcat agatgacaac gtatgctagg attcaggcgt cttcttttta
    17221 cataaaaagc aagtaagctt ccttttgttt tgctatatca ggttttatag tcaagcattt
    17281 tggttgacga tgaacgtgaa ctcgcactca cagacttttt tatcatctac atatgctgca
    17341 gataacgctg taccgactga tcccttcatt ttggtgattt caaaatgaca tttcagcaca
    17401 tctcctggaa caactttttg acggaatttg gccttattga tcccaccgat ataagctctt
    17461 tttcctagaa actcttctga ttttaggata agtatcgaac ctgcttgtgc cagtgtttct
    17521 agaatcaacg cacccggcat cactggattg tcaggaaagt gaccttggaa aaaatcttca
    17581 ttgattgtta cattttttgt cgcaacgatc ttttttcctg gttctaaagt atctacataa
    17641 tcaatataac aaataggata acgattagga atcaattcca ttacttcagt agcagataat
    17701 acgtaagaca taattaaact ccttctttca ggcaattgcc attttcatta ctttgactat
    17761 caaagtatca tattcctaaa tatttagcaa tcttttttta atttaagaaa aaatagtttg
    17821 acaatcaaaa cattttgact aataattaag aaatcaagaa gttattttag ctattattct
    17881 ctattttttt acgactatca aatttttatc agaaacaaag gagagtcatt atgtcatttc
    17941 tagaaggaaa aaaaatcgtc attatgggcg tcgctaacaa aaaaagcatt gcttggggtt
    18001 gtgctgaagc aatgttgaaa caaggggcag aaattatcta tacttaccaa aatgaacgaa
    18061 tgaaaaaatc attagtcaaa ttggtcggcg aagaagcatt cactgtagaa tgtgatgttt
    18121 ctagcgacga aagcatcgaa aaagcaatga atgccatcgg tgaatatgct ggggagatcc
    18181 acggccttgt ccatgcagtt gcttacgcca acaaagaaga attgtccgga aatgtgtctg
    18241 atatttccag agacggctat caattagctc aagatatcag cagctactct ttaattgctg
    18301 tatcaaaata cgcacaaccg tttcttgcac caaacagcgg gatcgttagc atgacttatc
    18361 taggttcaga acgcgcaatc ccgaattata acatgatggg tattgcgaaa gcttctcttg
    18421 aagctgctat ccgttatttg gcagctgagt tctcgcctaa aggaattcgg gtcaatggaa
    18481 tctcagctgg ggcaatcaaa acattagctg tcacaggtgt taaagactat caaaaactga
    18541 tccagttatc tgaatctcgt acgcctgatc agcaaggtgt gacgatcgaa gaagtcggta
    18601 atgcttgtgc tttcttaatc agcccacttt ccagcggaat catcggtgat attatctacg
    18661 ttgacaaagg tgtccatctt tcataaactt ttgttgcatt ctttaaagta aaacatataa
    18721 aaatcaggaa aaaacttttt aaagtctctt cctgatttt
//
LOCUS       DACDIH010000051        17968 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDIH010000051 DACDIH010000000
VERSION     DACDIH010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.051"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..518
                     /locus_tag="IV281_001890"
     CDS             <1..518
                     /locus_tag="IV281_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8780873.1"
                     /translation="AINRFYALLNALADEHVGNEELSLLKIALPSLLADAEYFEDFSI
                     LVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYEKEINLQLLSEKYHFSYSYLSAI
                     FTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEICEKVGYTELGYFSRVFKEETGMT
                     PSQYRKGTFVK"
     gene            515..2251
                     /locus_tag="IV281_001891"
     CDS             515..2251
                     /locus_tag="IV281_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8780874.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(2314..3150)
                     /gene="pheA"
                     /locus_tag="IV281_001892"
     CDS             complement(2314..3150)
                     /gene="pheA"
                     /locus_tag="IV281_001892"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP8780875.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(3147..3656)
                     /locus_tag="IV281_001893"
     CDS             complement(3147..3656)
                     /locus_tag="IV281_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP8780876.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(3649..4944)
                     /gene="aroA"
                     /locus_tag="IV281_001894"
     CDS             complement(3649..4944)
                     /gene="aroA"
                     /locus_tag="IV281_001894"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP8780877.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(4957..6048)
                     /locus_tag="IV281_001895"
     CDS             complement(4957..6048)
                     /locus_tag="IV281_001895"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP8780878.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(6065..7231)
                     /gene="aroC"
                     /locus_tag="IV281_001896"
     CDS             complement(6065..7231)
                     /gene="aroC"
                     /locus_tag="IV281_001896"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP8780879.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(7233..8297)
                     /gene="aroB"
                     /locus_tag="IV281_001897"
     CDS             complement(7233..8297)
                     /gene="aroB"
                     /locus_tag="IV281_001897"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP8780880.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(8330..9352)
                     /gene="aroF"
                     /locus_tag="IV281_001898"
     CDS             complement(8330..9352)
                     /gene="aroF"
                     /locus_tag="IV281_001898"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP8780881.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(10011..10985)
                     /locus_tag="IV281_001899"
     CDS             complement(10011..10985)
                     /locus_tag="IV281_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP8780882.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(10966..12849)
                     /locus_tag="IV281_001900"
     CDS             complement(10966..12849)
                     /locus_tag="IV281_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP8780883.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(12867..15092)
                     /locus_tag="IV281_001901"
     CDS             complement(12867..15092)
                     /locus_tag="IV281_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8780884.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            15207..16241
                     /locus_tag="IV281_001902"
     CDS             15207..16241
                     /locus_tag="IV281_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8780885.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(16308..17321)
                     /locus_tag="IV281_001903"
     CDS             complement(16308..17321)
                     /locus_tag="IV281_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8780886.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(17399..17587)
                     /locus_tag="IV281_001904"
     CDS             complement(17399..17587)
                     /locus_tag="IV281_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP8780887.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         4996 a         3905 c         3301 g         5766 t
ORIGIN      
        1 aggcaatcaa tcgtttctat gcattactca acgctttggc tgacgagcat gttgggaatg
       61 aagaactttc tttgctcaag atcgcccttc cttctttatt agccgatgct gagtatttcg
      121 aagatttttc tattcttgtc aaagaaacac ttgagaaact cacccacttt tctgcaaatg
      181 caaaaaaaga caatttcgat atcttgactt tgatctacga gttcgtccag gaaaattatg
      241 agaaagaaat caatttacaa cttttatccg aaaaatatca tttttcatat agctatctct
      301 cagccatctt tactgaaaaa tttggtatca acttttcaaa atatctaaaa aaagtccgta
      361 tcagtaaagc caagctcttt ttgacggaaa cacggcttac tttgacggaa atctgtgaga
      421 aagtcggcta taccgaactg ggttatttct ccagagtttt taaagaagag acaggaatga
      481 caccttctca ataccgtaaa ggaacttttg taaaatgaga aaaaaaatca aagagcatga
      541 actctttgcc aaacttctgt tcattttgtt catcggtctt tttctgcaag ccatcgtcat
      601 ttctttgttt atttatcatc gatcaaggga tgcctatatc cagctgttca atcaatcgaa
      661 tgatgtggta ctaaaaaaga ttcaatctga atttgaaagt ttgaatgata caatcgagaa
      721 tacattagcc gcttttgatt ctaatccagc tgtcaaaagc tatttttcca atgatccagc
      781 ccaacatatg gaacagcttc agcaactaag aacaatccaa aaaatgaatg actctctttc
      841 aaaaatccat ccaatgatcg attacgatgt attgatcttt ggagaaaatg gaagaacttt
      901 tgtcggaaac gacatgctca ctgccgtatc tgccgattct ttttttcaat ctgcgattgc
      961 tcagagagtg aatgaacgtg ctgctgatac acaaatgctt tttgcacacc acggattgac
     1021 tcttcgcgat aagaaagcac ccagcgtctt ctttgtcaaa aaactgaaaa atacattgaa
     1081 tcacgtttac ggatatgctg ttttatcgat ctcatccaag caactagcga atctttttca
     1141 atctgtagta aatcctgaga tatcgaagat ctccttcatg aacgaagaac agacgatcat
     1201 cgcatcaaat gaagaaaata caattgggag ccgatcaatg attttcaaaa aactaacagt
     1261 aggtgaaaca acgatttcaa atggacagca gttgacccgt cttcctctgt accgccagga
     1321 ttgtacatta ctcagtcaag tcgatcttca ttctttatct aaccaaatgg gcgtgatttt
     1381 tccgattatt ttgtataata tcgtgacact tgcttttgct ggctcattgg tatttatgta
     1441 tttggatcga cacacaaaat cgatctaccg cttgattgat tcgctaaaac ggctagagga
     1501 agatggctta aaagctactg ttcctgtaca aggaagccat gaagtccaat tgctagggaa
     1561 tacgatcaat caattgctaa gtaatgctca gcatcattat gaacagacga tccaagacga
     1621 aaagaagaaa cgttcgcttg aaatccaaac tatgcaagca cagatacagc cccattttat
     1681 ttataatacg ctgactagct tgaaattttt gatttggcag aaaaaaacag atgaagcagt
     1741 tcaaggaatc gacagtttca ttactttact aagaagtacg attgggaaaa aagaagaagt
     1801 gattccagtc aaggaagaaa tcaaaagcgt acaaagttac atcgatcttt tgagtatgcg
     1861 atatggcgaa agaatctcgg ctaaaataat gattcctgat gaactgcagt cattatctat
     1921 gccgaatatg atccttcaac caattatcga aaacgcctat cttcatgctt ttcacaataa
     1981 aacgcaagga ttcatcacgg tgtacggcaa aacaaaaaat cagcttcttt tattcgaagt
     2041 gattgataac ggcgatggct tcgatactga aaataccaaa caaaaaaatg atcatttttc
     2101 aggaatcggc atcagccatg tagatgagcg gatacgccta atgtatggcg atgcatatgg
     2161 cttaacgatc caatcagtga ttgggacagg aacgactgta acgatttgtc taccgcttct
     2221 tcctaaaaat caatcagaat atcaaaaata aagaatagcc aagcgctgat ttttttattt
     2281 taagttaaat aaatcaatca gtattcttga ctttcatcta cgcgtctctt ctactgtcag
     2341 cactggataa cagcccaata tttgactttc tcctccgagc atcttgattt cttggagcgc
     2401 attctctact aatgtcattg gacggtccaa caacaaatcg atcacgaaaa aatattctcc
     2461 caagctagtt ttcaatggtc gggattcaat cttacttaag ttgatttccc gccagccaaa
     2521 agctgccagc attttatgaa gcatccctgg ccgattagca ggtagtgtca agatgacact
     2581 catttttact ggagctgact gctggctggt catctttcgg tcaccgacga tccaaaaacg
     2641 tgtctggttc aactcattat cttggatatt ctcagctaag atctccaatc caacatgctg
     2701 tgctgtttct aatgaagcaa tcgctgctgc gtcttctttc ggatgttctg ctacatacat
     2761 tgctgcagca gttgttgatt ctgttgctac taaaggaaca ttaggataat gcgtttctaa
     2821 aaactgttgt gattgtgcta gtgcttgtgg atgtgaaagg atttttgtaa tctttgttgc
     2881 tggattgcct aaaagctgct gcttgatagg aagaacgatt tcacttttaa cttcgacttc
     2941 tggatgtttg gaaagaagat cgattgtgtg atgaacagct ccttccaatg aattctctac
     3001 aggaacgaca gccagatcga cttgtttgcg aaacaaagcg cgcaaacaaa cagggatgga
     3061 agcatacgca gtcaattgtt ctgtaggaaa tagctgacaa gccgcctgat aagtgaatga
     3121 actttctggc cctagatagc tgacgttcat atgcttttga tcctttctat aatggtatcg
     3181 atgatctttt ctggtgtttg attggtcgtg ttgattcgat gtgtcgcact ttcttcatac
     3241 caagaaagtc gttgtgccaa taatgctttt atttcttctc ttgtttttcc aagagctaaa
     3301 ggtcgaatag acgtttcatc ttgttggata cgatcaacta gacagtccgc ttctgcctct
     3361 aagtaaagaa caatcggctg tgtttttaaa aaacgccgat tttctgcact ttggacaatc
     3421 cctccaccag tcgcgataat tttgtcttca tttgacagct ttcttaaaat atctgactcc
     3481 acttttcgaa agcttttttc accgtaatag tgaaaatatt cggcaatcga caattggata
     3541 tgttcctcaa tctttgtatc taaatctaca tactcttttt gtaaacattg agctaaccct
     3601 ttaccgattg ttgtttttcc agcgcccatg aaaccaatca gcaaaatgct agccatgagc
     3661 ttctcctcct gacacaagtt ctgctaaatc gtcaaaaaat gccggataag agattgaaac
     3721 agcttcagct ccttctagtt cgcaaggtcc atcagctatc aaagcagcta cttgcagcat
     3781 cataccgatt cgatgatcgc ctctgctgga cacttttgcg ccatgcagag gtgtcggccc
     3841 atggatgatc agaccgtctg ctgtcgcttt tatctttgct cccattttct gtaattcttc
     3901 tgccaccgca tcaattctgt tggtttcttt tacttttaat tcttctgcgt ctttgatgat
     3961 cgtgattcct tctgcttgag tagccactaa agctagaatg ggcaattcat caatcagacg
     4021 cggaatgatt tctccaccaa tcgttgcttt cttcaaatga ctcgtcttga cacttaaatc
     4081 agccgattgg ttctgttcat tgtgattcgt ctgggtaatc gctgcgccca tctcctccaa
     4141 cacatctaag ataccagttc ttgttggatt gaccccgaca ttttttaaca acagttggct
     4201 ttccggaaca agaagtcctg ctgctagaaa gaaagcagct gatgaaatat cacctggaac
     4261 agttatctgt tgaccagtta atttttgcgg tccggtcact atgatcgttt tatcttccac
     4321 tgtgatcctt ccgccaaatt gacgaatcat ttcttccgta tggttacggg aacgttcttt
     4381 ttcaacgatt acggatgtgc cttctgcttg taatgccgca aacagcaaag cagatttgac
     4441 ttgcgcactg gctacaggca tattgtatcg gataggtgat aaagagcggg ttcctgtcac
     4501 agtgattggt agatactggt cattttcttc tccttgcaag tcagcgccca tctcgcgtaa
     4561 aggttccatc actcgcccca ttggacgttt agacaacgaa gcatccccga acagagtagt
     4621 cgtaaaaggc tgtccagcca aaatacccat caataaacgt atggtcgtac cggagtttcc
     4681 catgtcaatt gattcggttg gttggacaaa tgagtggcta ccgcgaccat gaatcttgat
     4741 cacctcttcc tcttcttcga tctcgacacc taattgttgg aaggcatgta aagtactcag
     4801 acaatcttct gcgcgcaaaa aattttgtat agtcgtcgtt ccttcagcaa ttgctccaaa
     4861 catgatgctg cgatgggaaa ttgatttatc tgctggtatc ctaacagtcc ctcttaatcc
     4921 atgtatttgt tgtaataatt gcatttatcc agcctcctat ttgatccagc aatgataact
     4981 ggtattagac ataatgattc tttttgcttg taacaaatcc tgttggtttt taaaactgat
     5041 ttgcagtatt cctaaaatat cttccctggt ctcttggatc ttcaaattaa tcaaagaaac
     5101 tcccgctttt ccgatcagtc cagttacttc tgcaataacg ccaggttcat ctggcacatc
     5161 gataaataaa tcaaaaaagg caggaatcgc gccttgctta tggacaggca actggtctcg
     5221 actttctttt gcccgattga aaaaagaaaa gatttccgat tggttctcag tcatgatcca
     5281 ttctgctatt tgtgtcattt cttgttgcca atctgccaat cgttccaata atatctgctt
     5341 attgctcaac aaaatatctg tccacatgac aggatctgag gaagcgatcc tagtgatatc
     5401 tcgaaagcct cctgctgcca gttgtttggc tcgcggatac tcttgattga attgttctgc
     5461 ctgattgact aagccagctg ccagaatatg tggaaaatga cttagcattc ctgttatacg
     5521 gtcatgttct cttgctgtaa gagttacata ttttgctttg gttccgcaaa atagttcttg
     5581 cagtttttca acatcttttt tcgtcttttc caaaggtgta aagatgtaat aggcattttc
     5641 aaatagttta gcatctgctg cctgtatacc tgatttatga gaaccagcca ttggatgtcc
     5701 accgacaaac cgaagatttt tctgctcagc ggatgtcacg acttgctgct tcgtactgct
     5761 gacatccgta atgatgacgt gatcagacaa gttgattctt tcaaggacat caagataagc
     5821 taaggttgta cggacgggaa cggctaataa gataacgtct gcttgttctg cacctgtttc
     5881 aaaatcactt gcaacgtaat caacgatctg ctgttctact gcttgctcac gggtttttga
     5941 ctgactatcc caaccaacta ctatcagttc tggattggtc tgacgaatac aaagtgccat
     6001 agaagcacca atcagtccaa gccccacgat aaaaacatgt tgtgccatct tatttgcccc
     6061 ctttttagta atgtttcaag tagtcacggt actccttcac tgcagctttc aactcaacaa
     6121 gcgaatcact tgggaatttt gacaatagct ctgtcgctac cacagtggct acaacatttt
     6181 ccgcaacgac gctagctgca ggaactgctg tactatccga acgttctaca cttgctttgt
     6241 aaggttcttt tgtatcgata tcaacacttt gaagtggctt gtacaatgta ggaatgggtt
     6301 tcatgacccc gcgaatgaca agtggttcgc cattggtcat tccgccttcg aagccgccta
     6361 gattattggt acgtcggaaa tagcctcttt cttcttccca taggatctca tccatcactt
     6421 ctgagcctgg taatgaacca gaagtgaatc cgaccccaaa ttctacgcct ttaaaggcat
     6481 tgatactaac cacagcttgt gcgattttgg catctagctt cgtgtcccat tgtacgtagc
     6541 ttcctaatcc agctggaacg cctccaacga gcacttctac aattcctccg atcgtatctc
     6601 catttttctt tgtctgatcg atcaaatctt ttattctttg ctcaactgct ggatcaacaa
     6661 ctcgtacttc tgacgcttca gaaatttctt tgatttcttg aatcgagata tttccaggta
     6721 tttctgcttg gattccacca agacaaatca cgtgtcctgc tacgatgatg tcaagttctt
     6781 ccagaatttt cttggcaatt gcgccgattg ctacacgcat ggtcgtctct cttgcagagg
     6841 accgttcaag cacattccga agatctcgat gatggtattt cataccgcct accaaatccg
     6901 cgtgtcctgg acgcggacgt gcaactcgcc gcagtgattg gtctttttca ggcacttctg
     6961 ctacagacat gacagttgtc caatttttcc aatctttatt ttctaccact aaagtgacag
     7021 gtgaaccaat tgttttgccg tgacggatgc cagacgtgat ccgaacacga tctttttcga
     7081 tcttcatcct tcctcctctg ccgtaaccag cttgtctgcg agcgagttcg tgattgattt
     7141 cttcttctga cagaggtaat cctgctggta gtccttcaat aatggctgtt aattctggtc
     7201 catgggattc tcccgcagtc atataacgca tcttacagtc ctcctaagta atctttgaat
     7261 gtctctgtgg gaacaggaac gatttttgct tgcccaatct tttctagcaa gatgatattc
     7321 aattggcttc ctcttgcttt cttatcatgg gttatcgcgt ggaataattc atctggatga
     7381 cttagatcgt acgtaatcgg cagatggaat ttttgaagta tcctttctaa agcagctgtc
     7441 gatccagttg gagacaagcc gattttttct gcatgagaag tgatggctac cattccaata
     7501 gctactcctt ctccatgact gactacccca tatcctgctg tattttcaat tgcatggcca
     7561 attgtatgac caaaatttaa aataagtcga tttccttggt caaagacatc ttcttccact
     7621 acttttcgtt tgatttccaa cgtagcagta atgattggaa cagcatgttc cagaagatct
     7681 ttttcgtctt tcagctcgtt cagctgtttc caaagcgttg catctgctat agctgctgat
     7741 ttgatgattt cagcgattcc ttctcgtaaa cgacgtaccg gcaaagtttt tagaacctca
     7801 ggatcaatca atacaccagc tggctggcta aatgttccga caaggttttt cgctttgttt
     7861 gtattgactg ctgtcttccc gccaatacta gaatctactt gtgcaagtag cgtagtcgga
     7921 atttgaacga aatggatccc acgcatgaac gtcgaggcca cgaatgctgc tagatcaccc
     7981 accattcctc caccaagtgc aagtatccca tcgctgcggg taaaatcatt atctgctaaa
     8041 aaatcgtata actcagctgc ttgttctaaa gatttactgg tctcacccgc cggtacgatt
     8101 gaaaggactg gttcaaaatt agcttgtctc aattggaaca tgattttttc tgcatatagt
     8161 ggagcaacgt ttgtatcagt gatcacagct actttttgag gtctccaaat cgtttgaagc
     8221 catgatccag cctgttctag tgcgcctttt tcaatatgaa tatcgtattt ttggttatca
     8281 acatgtacag taagcattcg tttcacttct ttcttttaaa aagctggaat taatcgagtg
     8341 tctgaatctt ttccattgct tctttcatga tcgcaacttc tttcatcatg cgtgagtaag
     8401 tcttttcatt caacgattgg ggaccgtctg accaagcatt tgcaggatca ggatggatct
     8461 ctacgatcat gccatctgct ccgcttgcca cacccgcacg agccattggt ggtacaagtt
     8521 cccaaattcc tgtaccatga cttggatcaa caatcacagg gaaatgagtt aattttttca
     8581 tcaatgggac agcacttaaa tcaaacgtat tacgtgtcgc tgtttcgtaa gtccgaatcc
     8641 cacgttcgat aaaaatgaca ggagatttgt cttcgactgc aatgtattct gcagcattaa
     8701 gccattcatt gatcgtaccg gaaattccac gtttcaatcc aacaggttta cctgttttac
     8761 cgacagcaga tagcagttta aaattttgca tattacgtgc gccaatctgt aggatatcgc
     8821 tgtactcagc caccatatcg atatgccctt catccatcac ttcagtaatc actttcattc
     8881 caaattcatc agccgcttga cggatgtatt tcaatccttc ttcttctaaa ccttggaaag
     8941 catatggaga agtacgtggt ttgaaggcac cacctcgcag aatttttgcg cccccggctt
     9001 tggccattcg cgcacattct ctgatttggt ctaatccttc gatagaacaa ggaccagcca
     9061 tcgtcacgaa actgccgtca ccaattttta ctccgtctac atccacgact gtattgccag
     9121 gatggaattc tcgagaagtc aatttgtatg tgtttaaaat acgcacagca ttttctactc
     9181 cttcatagcg tgtaaatgct ccttcttgta aacttcgcgt atcacctttt agtccaatca
     9241 ctactcgttc tgttccttga tcgattgaga ctttgaatcc ttctttttcg atatgatcga
     9301 cgactttttt tatttgttct tccgttgctt cttttttcat aattactatc attgtttttc
     9361 ccgctttctt ttgtttactt atctatcttg gctgattttc tctacttttt caaggtggtt
     9421 ttacattaat tcctactaaa tgaccgtgtc ctgttgttct cattttcgct ccttctttcc
     9481 tacttatgac ccagataatc aacaaaaaaa gatgtccctg taggaaattg cctctacagt
     9541 gaacatcttc aacgagcctt aaaaagcgct gcaaaaatat aaaatagcca ctatagatga
     9601 atttctctac gtggctgtaa gtcgtgttta aaaatcttta gccatcatag atacaaacct
     9661 ggtttgtttg tacccacgaa gtcatgaata caaacctatc taaaatagct aaagtaatac
     9721 gtattcagtt ttgtaagttt tctagatttc ataaaaaatc gctccaaact gatgtaagaa
     9781 ttgtacctag tatagacata aaaaaatcga ctgtcaatca tttttatgaa acttttttta
     9841 attttctgat agttaacttt gtttttcaga aaaataacaa ttggttttat tgaagaaaat
     9901 agtcatgaac aaaagtatct gagaactttt ttactctctt gttcatgaca tatagaataa
     9961 agcagctttg tcttgtaaac tccgtttttc aaataataat ctaaagattg ctactgaata
    10021 ataaagccga tttcatggtc ctcttcgctg ctgattcggt aaggtgactc aacatccgct
    10081 ccccagctat tgataccccc gactcctctt acttttgcaa aaatcgtaag aaccgttcgt
    10141 ctttctggcg gtagttcttc tatatgtgtt gcctgttcca gttcttctgc tttgaatggt
    10201 aaagcggaaa aagcaaatgg cgtatcctgc tgttcaaatc gcaacgaaaa atcttcttga
    10261 tctttatccg tattatccaa cgtttgattt cttgttagtt ctacccattt cgtttccata
    10321 tgcatgccgc attcttgggg aacaaggtat ggggaaacgg ggagtcctgc cactttataa
    10381 ataccttcga caccaccagc catccgatcc ggatatgttt cccctgatag gccttcatac
    10441 tcaaaaaatt ttgctttcgt tggcataatg aaccttaaac ccaatgcggg taattccggt
    10501 aatccttttt gtccataata atgcatattg acaagcattt taccctctcc agcgatttgg
    10561 taggaaattg tcacttgggt ttttggtact gtgttgattt ccagtgtaaa aatgatttca
    10621 accgtttcag cgtattcatt acttgaatat tgattattta ctggagcaat cggcaaatcg
    10681 atctttttat tgtccaccag tacttctatt tctttgactt ttggatacaa tccggctcct
    10741 aaccacatac cggatttaaa tccaaatccg ctacctctgt cattatcggt cgttgctcgc
    10801 caaaaaactg gaagtgtttc ccggtatagc cattcttttc cgtttttatt gagcgattcc
    10861 agtccgcctc ttgtataaga aaaaatataa tgcgtattcc ccgatttgac acctaatgta
    10921 cagtcgccga agacgattgc aactttttcc gtatccatat gacttttatt tactgtattt
    10981 ttcataataa tatcttacct cctgcatggc gggtttttct tgtccatctg caaaaacgat
    11041 cccattccct gagaattcat agtcacttgg tttatcgtca aagtcaccgc catagcgcat
    11101 cacttctctg cctgtgattg gatcagtcac ttggatcgcc tgatctttaa aatcccatat
    11161 atagcctcct tggtacattt cataacgatc aagcaacgtc atgtaagaat ccattccacc
    11221 taacgaattc cccatgtcat gcatatattc acataagatg aacggttttt ccgctttatt
    11281 ttcaaggtag tcaatgatct cttctggtgg cgcatacatg cagcttttta catctgaaat
    11341 cgaatcttcg aatgctggat ttggaaaatt cccttcataa tggacaagtc gatcggggtc
    11401 tacttttttg aagtaatcgt tcatttgtcg aatgttttct tccgcataag attcatttcc
    11461 aagagaccaa aaaagaatac ttggatggtt ttttaatgtt tcgaaattcg tttttgctcg
    11521 atcaagaaca gccgcctgcc actgaggtac tgcacctggt acgttgtaag aaggctcaaa
    11581 tgcgcccatt ttttgccatg agccatggga ttcgaggttc gtttccgcca tcacatggat
    11641 tcctgcttgg tcgcataaat aataccaagt gatctggttg gggtaatgag atgtccgtac
    11701 gccgttgatc tttgcatttt ttatgatagc caaatctttt tccatatctt ctatagtgat
    11761 ggcgcgtcct tttttcggat gccactcatg tcgatttacc ccgttgataa tcatccgttt
    11821 tccattgaag tagatgattt tatctttgat ttcgatttta cgaaaaccga aggaatagtc
    11881 aatatactcc gttactcttt tttcttcgtc tatcgtctcc aattcaagct tatataaatt
    11941 gggctgaaaa tgcccccaca gtgccacatt ttcaaatcca tcgatgcgaa tcgtcatttg
    12001 ctcttttaac ggatattgct gttgatagat ttccttccca tcaggatcta gtatcgctac
    12061 attcagctga ctgtttttgg ctacttgctc aaaagacaaa tctacttcaa gtttgccatt
    12121 ttgacatttg tcatctaagc gaggtttgat ccaaagatct tctaaatggt tttgtcctcg
    12181 agcaactaag aagacatctc tgaagatccc aaaaaatcgg aaaaagtcct gatcttccaa
    12241 gaatgcagct gtacttcttt tgtaaacctc cacagctaga atattttctt cttccaaata
    12301 aggtgttaaa tcaaattcag acggagtgaa cgaatcttcg gcatacccga taaaatgacc
    12361 attcaaccaa ataaacattg cttgttctac accagcaaaa tgaatcgaga tttgttttcc
    12421 ctttagacct tcttctaatc gaaaagtcgt caaatacgaa ccaaccggat tgtctttagc
    12481 agctgaaaag atcccttccg cctctttctc agggcttgtc gtgaaagggg gacggcgaaa
    12541 tacatgtccc tcccacggat acatcgtatt gatgtaatgg atcttgtcat aatttgctaa
    12601 ttcaatatga ctaggaacag tgatttcatc aaaagaacag gcatcaaaat cttttttata
    12661 aaatgttttt ggtcgtaccg aaggatttgg cgaataacaa aaacgccatg atccatttaa
    12721 agactgttta agggaggatg tggtttctac ttcttccctt gttgcataac aaacatgatc
    12781 gctatgtgca tttgattggt tcactctaaa aacttgtgga tcatctaacc attttatatt
    12841 tgctttcata cttttttcct tctctctcat aactttttga aatgaagcag ttctgtttgg
    12901 aaatcacctt gaaaatcggg gaagtaaagt ccaactgtca tcaattcggc ccccgaaaat
    12961 atccgcttcg aggtctgttc ctgataaagt gcctcatcat ctaaccccat cagatatact
    13021 tcatggaacg ctggttgagc tgaagctagt atcctcccta aaaacacaat agcttcatct
    13081 tgtttaggag acacaaacat ccatgcggca atattttctt caaaaggact cgccaaacga
    13141 taataatggc catactggat caccggacga atcgtttgat aggatataat ttggtctttt
    13201 acttgctttt tttcttcagg agacagcttt gtcaaatcca gttcataacc aaaaacagcg
    13261 ctcatagctg tttctcctct tgttttcaac gaagtagatc tgtcggtctg atgatttggt
    13321 acagctgata catgagctgt gatggacgaa atcggataag caaggcttgt tccatattgg
    13381 attttcatac gctccactgc atccgtgtta tcacttgtcc atgactgcgg catataataa
    13441 ataaacccgg catcaaaccg tccacctcca ccagaacagc cttcccataa gatttccgga
    13501 taatcagttg tcaaatgttc aagtagttca taaagcccga gcatataacg atgcgctact
    13561 tctccttgtc tctgtgggtc aagggtaatc gaatagacat ctgaaagcga acggttcata
    13621 tcccatttga tgtagtcaag aggaatcgta tctaaaatct ttctcatttg tttctcaatc
    13681 gtctgccgaa catctaacct tgtaaaatcc agtacatgtt gtgatctgga agcagaaggc
    13741 attctacctg gctcctgcat aagaaaatca ggatgtgttc gatacagttc agaatcaacc
    13801 gagatcattt caggttcaaa ccataaacca aatttcaatc ctttgctatg agcataatct
    13861 gctatttcac gtaatccatt tgtcaacttg ccttcatact cgaaccagtc tcctaatgaa
    13921 gaattgtccg catctctttt tccaaaccag ccatcatcta atacgaacat ttcgatgcct
    13981 agctcctttg cttcatcaat gatcgccttg attttttcac tattgaagtc aaagtaagtg
    14041 gcttcccagt tattgaccag aatcggccgt tcagcaaatt gatgacgacc tcttgcgatt
    14101 ctttcacgta gcaactcatg gtggatctga ctcattttgt tcaatccctg acttgagtaa
    14161 gaaaggatca tttcaggtgt ttgaaaagac tggcctgcat tcaattccca cgaaaaacga
    14221 tggctgttga tcccaccgac gactctcaat tgattgatct gatctttttc aagttcgaaa
    14281 gaatggttgc cggaataaac aaattgaagt ccgatcgctt ctccagtatt ttctgtcgta
    14341 tgatgatgaa ctaaagcaat aaagttattc atatggtgac tgctcgttcc tcgacggctt
    14401 tcaaatactt ttgttccaga taaaacagat tgccgtgaaa tctgtctttc attgacatgt
    14461 gctcctggca aagcaatgac ttcatcaaat cgcctagtat gggcaaagtc tagctggaaa
    14521 ctagccgctt tttctagttt gattgtttct ccggtttcat ttcttatctt tacagatcgt
    14581 gtgatcactg ctctatcagt aaatatggta tagaacaaat cgaaataaat cgctgtttct
    14641 ctatccttaa gtgaaatcac caacgtttgt gcctctgact tgtccatcac ataagtttga
    14701 ggcaagcctt cgatctctgg cttcccatct gtaatataat ggctgtcata tctcagatcc
    14761 agtaagtctg atccattctt gcgctgaatg atcgatgcgg gcgttcgaaa gtccatgctg
    14821 ccatgagaag agtattcttg cggtagtgta tctttcgaca gagaccggtc aggatttaag
    14881 ggaacatttc ctgaaaatcc acgatctctt cggggataat ttttattgtc tttgtactgc
    14941 ttgactggtt tgccaaaata aatatgcgcc aatacattgc tttcttctat ctttatcaga
    15001 taagaaagaa atgtatttcg taaatggaag actcggctct tttcatcaaa taagatgaaa
    15061 tttttatctg tttttctatg cgtaatatcc atcatttcac tcccaacaaa acggaaaccg
    15121 tttacttttt tttataattc ctgtatactg tttactaaac aattttgtca ataaaaattt
    15181 tttatttttt tgaggagtgt gtaaaaatgg ttggaattag agatgtcgca cgaaaggcaa
    15241 atgtctctcc tgctacagtt tcccgtgtgt tgaataacga tcagacgatc catgtggctc
    15301 agactactcg gcaacggata gtagaggcag ctgctgcgtt ggattatgac atcagtactc
    15361 gtaaatatat caaaaagaga atgccttcga ttggtgtgat ctctacgatc ggcaaagcga
    15421 gcgaagaaca ggatatctat tacaaacatt tgcgagtagg gatggaagaa gaagctcgac
    15481 gtctgcattt agggatgaat cgaatctata atctatctga taatcctaaa caatgggaag
    15541 atctagatca gttaggtgcg gtgatcgttg ttgggactgt tacgaaagaa tcgattaccg
    15601 ccttgttaaa acaaaacatg caaatagtcg tcgtggataa ccctgacatc cagcaagagg
    15661 tcgatatggt gtatgcagat cttgaacgga tgactaaaac cgtactggaa ttgttcttag
    15721 aaaatggtca taaaagaata gcctatatcg ggggatataa agtcgatatg gatgaattcg
    15781 gtgggaagca gatttctata aatgaaaaga ggatgcgtgc ctatcagcat tttatgatcg
    15841 atcatcactt agaaaaatat atgtcttatc atctaggtgg ctggacagaa aaacaaggtg
    15901 aggtacttgc gactaatcta ttagaaaatg aatcccctct tcctactgcc attcttgtcg
    15961 ggagcgatcc aatggcaatt ggtgtctatc gggcattgaa acaaaaaaat atccaaattg
    16021 gagaagagat tgttgtagca agctttgata atatcgagac aatcgcaaac ctctcccctg
    16081 gcttgaccag tgttcaaatc aatgcgaaat caattgggca agcagctgtt cgaatggcat
    16141 tggagcgaat tgacggtata cgggacgaac caatcatttt gacttatcct gcacgattga
    16201 ttgttaggga aagttttatc ccaagaacaa aaaggtattg aaagagaggt tgtgaaaagc
    16261 cgcttcgttt cttcatactc aaaacgaaac ggctttttca caacctctta cttatactta
    16321 ttcacaaaat ccaatgtctg ctggtaacaa ttttgcgatt cttttagcgt gtaatcaaat
    16381 tggtattcat gggtaatctc tttttttgta tctgtataaa acaaggattg cacgggtacc
    16441 tttaattctg tcaaccgatt ttctaatgct tgaccttgct cctggaatga gtatgcattt
    16501 ccgtcagtta tatacgttgg cggaaattcc ttagaaacat ggtcagccac actagcttct
    16561 ttcaattgct cactcgtttt ccaatctttt gttccaatca acgaccacgc aacggttttg
    16621 acgaaaaact tcataaatcg atcatccgaa tgctgctgtg ctgttagctt caaatcaaca
    16681 ggtccgcaat aagaaatggt tcctttcaga ctatcttttg gaagtagctg tttgattttc
    16741 atttgtttag catacgcatc attcgtttgt atagctgcat attgcaatgc gatttgtgaa
    16801 cctgcactat ctccaccaaa aaataattgt tccatgtcta gcaatttgtc gtctttttct
    16861 tcagctaata aatctttgat cagttcattc acttgaagaa cttgattagg gtattctgaa
    16921 tcaggcgcta attcataatt catcgcaacc actgcgatgt tggcgtctga aactaatttt
    16981 gtcgcaaatt ctttgacacc cgatttatct ccgccgacga atcctccacc atgtacccaa
    17041 aatagaacag gaacaggccc cttgctgttt tttggatagt agatgtcata agtattctct
    17101 ttatacttgg actcatacgt caaatctgtt tttttcgcca cgtttttttc tgcttgatcg
    17161 aacgtttttt tatcagtgat ttgaacagta tttgaaaaca tatggttgat caagaatgct
    17221 ccaggtctgg gggaaagctg gaaggcaaga aagattgcaa acgcgatccc aagaatgatt
    17281 ccgaagaacc atccgataaa tttaagaatt ttctttttca taaggctccc tcagtagtta
    17341 gtattaagca ttctgattat accatagtgt tatttcctat caagctctcc tagatgattt
    17401 aatgattttc cccaaacttc ttctgcaatg cttttgacaa aagaaatttt ttccattgtt
    17461 cttcaaacaa aacattttct tcttctgttg aagcaaatcc acattgagga cttaagcaca
    17521 aacgatccaa aggcagatat cgtgtcgctt cgtagatccg gtcaatcact tgctggtgct
    17581 cttccatttc gccatttttc gttacagtca aaatctaaga caactaattt atcttcagag
    17641 actttctcta aaagaacaaa actcccagca cgttcactat cgtattctaa ataataggca
    17701 cggacatttt cttgagtaaa ataatgggtc tgctacgtcg tcatcccccc tgatgccgcc
    17761 aaaatcgaat aataatttcc gcgtcaaaca tgtatattta gtgttgaatc atttgagaat
    17821 atgaaaataa aaatcttttt gatcaatcag ttaatcaaat tgaaataacg aaaaatctga
    17881 gacagaatga cgttcattct gacccagatt tgattttgca caactcaaca gtccttattg
    17941 gcaaatcgcc aaaataatgt agactaac
//
LOCUS       DACDIH010000052        17843 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDIH010000052 DACDIH010000000
VERSION     DACDIH010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.052"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            648..965
                     /locus_tag="IV281_001905"
     CDS             648..965
                     /locus_tag="IV281_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP8780888.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1056..1421)
                     /locus_tag="IV281_001906"
     CDS             complement(1056..1421)
                     /locus_tag="IV281_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780889.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1629..2111
                     /locus_tag="IV281_001907"
     CDS             1629..2111
                     /locus_tag="IV281_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780890.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2308..3198
                     /gene="pstS"
                     /locus_tag="IV281_001908"
     CDS             2308..3198
                     /gene="pstS"
                     /locus_tag="IV281_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP8780891.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(3485..4759)
                     /locus_tag="IV281_001909"
     CDS             complement(3485..4759)
                     /locus_tag="IV281_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8780892.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            5032..7503
                     /locus_tag="IV281_001910"
     CDS             5032..7503
                     /locus_tag="IV281_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8780893.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(7606..9342)
                     /locus_tag="IV281_001911"
     CDS             complement(7606..9342)
                     /locus_tag="IV281_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP8780894.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(9339..10043)
                     /locus_tag="IV281_001912"
     CDS             complement(9339..10043)
                     /locus_tag="IV281_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8780895.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            10174..10677
                     /locus_tag="IV281_001913"
     CDS             10174..10677
                     /locus_tag="IV281_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP8780896.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            10637..10975
                     /locus_tag="IV281_001914"
     CDS             10637..10975
                     /locus_tag="IV281_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP8780897.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            10996..12414
                     /gene="ffh"
                     /locus_tag="IV281_001915"
     CDS             10996..12414
                     /gene="ffh"
                     /locus_tag="IV281_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP8780898.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            12526..13104
                     /locus_tag="IV281_001916"
     CDS             12526..13104
                     /locus_tag="IV281_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP8780899.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            13108..13629
                     /locus_tag="IV281_001917"
     CDS             13108..13629
                     /locus_tag="IV281_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8780900.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(13708..14262)
                     /locus_tag="IV281_001918"
     CDS             complement(13708..14262)
                     /locus_tag="IV281_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8780901.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            14515..14790
                     /gene="rpsP"
                     /locus_tag="IV281_001919"
     CDS             14515..14790
                     /gene="rpsP"
                     /locus_tag="IV281_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP8780902.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            14802..15053
                     /locus_tag="IV281_001920"
     CDS             14802..15053
                     /locus_tag="IV281_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8780903.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            15178..15969
                     /locus_tag="IV281_001921"
     CDS             15178..15969
                     /locus_tag="IV281_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780904.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            16139..16660
                     /gene="rimM"
                     /locus_tag="IV281_001922"
     CDS             16139..16660
                     /gene="rimM"
                     /locus_tag="IV281_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP8780905.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            16650..17411
                     /gene="trmD"
                     /locus_tag="IV281_001923"
     CDS             16650..17411
                     /gene="trmD"
                     /locus_tag="IV281_001923"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP8780906.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            17547..>17843
                     /gene="rplS"
                     /locus_tag="IV281_001924"
     CDS             17547..>17843
                     /gene="rplS"
                     /locus_tag="IV281_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP8780907.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYY"
BASE COUNT         5888 a         3175 c         3612 g         5168 t
ORIGIN      
        1 cttattgctt ttgcaaaatt tatttacctg ttttctggat caacgatagt gtacattatg
       61 ctatccttgt ttggatttta ggacgtttaa agaggcactt ttttacataa cttaatccat
      121 agacctttta agcatgctgt gagataataa taaagaagag tttaaagcgc accccaaacc
      181 acttccccat aagtgtatta cgctttaaac tcttttatat ttgaattcac taagaagcat
      241 accatatatt tgaatttact aagaagcata ccatattttt gaaaaaaagt gagaaaaaag
      301 gcttataatt aggatttaga gtaattaatt agatgtttat tttataggac tgatactata
      361 gattacaaat agtatcgtat ttcgcaatct taaaattatc ttcttactgt gaataagata
      421 ataaaatttg tatttgtcct ttttaggtaa cttattttaa tctatatacc tttctggatc
      481 aacgaaagta tactttatat agtcataacg ccgatggtaa ctatgcactg taacgataat
      541 gctgctaaaa tatcttttta cgaaaaacat cacaaggaaa aacttaacta tggtattatc
      601 taaacataat tttttcggta tttaatattt aattaggagg taatattttg cttttttata
      661 gtaattttat cttaatagta gcgatattat tacttctgaa tatatggatt tttgatagga
      721 gtagaaattc aagtataggt tttagaacta aacgaagttt gtcttctaaa aaaaattggg
      781 tatactcaca aactattttt tatggaggaa ttgttttaat tagtttactt tcttcaacac
      841 tttattcttt gaatattata gatgtttcca cctcaaattc aatatctatt attggaatca
      901 ttattgctgc aattattaca caactgtttt tagtgtttgg agagaagaaa agaagtaaaa
      961 aatagaaaaa ctagtgatag ttttggtgat ataagaaaaa catatatgta taaaaagaag
     1021 aaagtagctt ctctttgtac atatatggtt tgtttttatg gatgccgagc ttttttataa
     1081 gataaggcta ccgcccataa cgacccaagg atttgtggaa ctaagtaaat acctgcaaga
     1141 agcaagatca gctgggtcgg ttttaaagga tgatagaagt aacgccattc agtaaaattg
     1201 gcgcaaaata aataggaagt gacagtagat ataatattgg caatgaataa agatataata
     1261 cgcctgttcc tttgaaaata atatccagca aaaagagata aaaatatcaa tgtgatgaac
     1321 ccgcttacgg aatgataacg aaaatcaagc agtaaggcga aatgcacata aggaacaaaa
     1381 gataaaagca acggaaggtt tttccagcaa gtttttatca tctttcatca ccacctaaca
     1441 agtgttatta ttttgttagt tttattgtaa attgaatcct gcaataatca attacttttt
     1501 cattatttct agattaaatg aataaactct catgccatta tctctaaaaa aaaagttttt
     1561 ccccctaatt tttttagata gaaaattgta tagtatgatg agagaaaaag tagtttgggg
     1621 gaacgttaat gagaagaaaa gcagtaatcg atcggcttta tttatttttt tggttttttc
     1681 ctatcgtttt gctacctttt acatgggtaa atgctgttgt acaaggtgcg gagatattag
     1741 gcctgatcca aggaagcgga tttgtggttt ctaggaattt tccagctttg attgttttct
     1801 atttagtgat aagtgtaggt ctcttattac caaaatctct gatcaatcac tacaattggc
     1861 agctgcttta cttttcttgc ttattagtat ttacatctat gttttctttg ctgcctcgct
     1921 tgattatcgg ccctaatggt ttgagcgaga gtggattgaa ggatttagct tattcctcca
     1981 tgtatttctt gacgatcaaa ccatggttct atttagtaat tgcctcattt attcttgcaa
     2041 cattttttta cttctggaca gaaatcagga taaaaagtga acgatctgta tctataaaaa
     2101 ataactattg atggtggatc atcagtaaaa aaacatatga gctgcgtaaa atgcatgttt
     2161 ctcatatgct ttttttatta ttatgtaaag aatgcgaaaa acgtttacat gatttttaca
     2221 taacttaact atatttttac agcaaccata cgagggattt acagtcgact gataaacttt
     2281 agttgtaaat aagaaaggag caataaaatg aaaaaagtat tgtttactct aggtatgatg
     2341 ggaattcttt tagccggatg cggctcagga tcaggaaata cagatggctc tgccacacaa
     2401 aataattcaa gtgactcaaa tgaacaagta aaaatcgttg cggtaggttc gacagcatta
     2461 cagccgttag tcgatgcagc acaagaaagc tttgtacaag aaaatccaaa ttatcaaatc
     2521 tctgtacaag gaggaggaag cggaactggt ttgagccaag tcgaagctgg agcagttact
     2581 atcggaaact ctgatgtatt tgcagaagaa agagacggag ttgatgcttc aaaattagtg
     2641 gatcataaag tagcagtggt cgggatggca ccaatcgtca ataaagatac agatgtcaaa
     2701 gatatcacca aacaggaatt gattgatatc tttacaggaa aaatcactaa ctggaaggaa
     2761 gttggcggga aagatcaaaa aatcaacgtc gtgaaccgtg caaacggcag cggtacccgc
     2821 gcgacattcg aaaaatgggg actggatggt gctacccctg tgcagtccca agaacaagat
     2881 tcatcaggaa cagttcggca gctggtaagt caaacaccag gagcaatcag ctatttagct
     2941 ttctcatatc tcgatgattc cacacaagca ctaagtatcg acggtgtaga accaaaagaa
     3001 gagaatgtag cagataacag ctggggaatc tggtcatatg aacatatgta cacgaacgga
     3061 aaaccttccc ctgaagtcca aaaattctta gactacatga tgacagaaga aatccaagaa
     3121 ggacctgtca aagaattggg ctatttacca atcacaatga tggaagtaga acgtgatcac
     3181 gaaggaaata tcaaataact agcagataaa catgccacgg tagtcgttac tattaaggaa
     3241 aaatcaacta tgtttcccca catatacatt tttcctttaa ttgtgaaaaa tagttcctca
     3301 tattttgccg ttttttgagc taataccgag ggtcagctga ttgaacaccg tttattcgaa
     3361 aaaaagagct acgacataga actgtcgtaa ctttttttgg ctcttcgtca aatcgtgttg
     3421 gtgaagacaa atttgaataa aacttagtcc gatatgcggc gcgttctgtg cgccgcatat
     3481 ttttttagtt ttcaaagatc agaccttgtt gtaaatagat ccgtgcccga aagttataaa
     3541 agttgcgata accaaacgca attcgtttaa tcagcttgat cttgttattc agcccctcag
     3601 tgacgccgtt tgagtgggac accttaaagg catttgaaat acccgattgg tagcaatgga
     3661 agatcgtgaa tttcttttta aacgactgag gtaacgtaga tgaaattgtt ttaagggttt
     3721 cttgaaagcc ttccgtacct cgtttaacga aataatagcg taataattga caaaaatgat
     3781 acgcagttcg caattcttcg ttgtagctta acagctcatc aacgatttct gtttgactaa
     3841 ttggtcgttt aaacagtcga tgataatgcc gtttgagtgg ctcccatgtc gtggaatctt
     3901 tgagtaagag tttccaatat cgcttgagcc gacggtactt cttctgattt tcagagtgtg
     3961 aggttcgaaa acgattcatg atctggacac gtaattgatt gaagctgcga ttgatgtgtt
     4021 ggacaatatg aaagcggtca gttacgatct ccgcatttgg aaagactgtt ttaagcaact
     4081 gacaatagct ggcattcatg tccatcacta ggtacttgac ctttaaacgg gcctttctag
     4141 gaaagcgcag gaagtattga gttagtttgt aaagtctgcg atcttcaagg atcgtaaata
     4201 gctgattggt tgtcccatca acacaaataa aactcatgga accagaaaca gaattcattg
     4261 atttgaactc atccatacac aacaccgtcg gaaggaagtt catacgtggc tggtaatgct
     4321 tcgttgcttc acgcaagatc cgtgtcaccg tcccatccga gacgaagtac ttcttagcga
     4381 ttagtttacg tgattgattt tcgtacagtt ccatgagaat cgcctgcttc agttcctttg
     4441 ttagttgatg atgttcatca actaagtccg ttttcgcaga aaaggtcctt ccacaatcat
     4501 ggcagagaaa acgggtacgt tttaattcta aaatagttaa cttttctttg actttcaaca
     4561 tctgagtgcg tgtcgtatag gaaccgttgc ggataatacg aaccgatcga cagtgttcac
     4621 aataggaaga agagtcatcc aattttcctt taataaactg cgcttgaacg cctttatatt
     4681 ctttttcaat cagccattct tcatcaaaaa ataaatgttt atctgttaat ccgagtaatt
     4741 ttctagtatg attatccatt gagagactcc tttggtagga tgacgttttg gtcgactaaa
     4801 ttttaccaaa agtgagtctc tttttgagtt gaaacgaaga aaaagtgtta gtagaaaatc
     4861 gccttcgatt ttccaccaac acaaaatatt atagaaccct ttttttttgc tatatgacag
     4921 gaatgaaagg tgtttgcaag cttaatgtga ggaacacttt ccatatctgt tctatactgg
     4981 ataggagaag tttggaaata tccagaaaac gaaatcaatc gaggtagcca catgaagaat
     5041 aaatggataa atcgaagtaa aaaaaagaaa gtaacacaaa gatcatcaga taaagtcatc
     5101 cgtctttttc catcgtataa agaaggatta acggcagatc aagtcaagga acgaatagaa
     5161 aaaggtgcag caaataattc agtcgatccc acattcaaaa caaatcagca aatcgtattg
     5221 gaaaacatat ttacgtattt caatttgatc tttcttatct tagccatttt gctttgttta
     5281 gtagaatcct ataagaactt gactttttta ccagttatca tagccaatac aggaatcgcc
     5341 atttttcagg aaattcgatc caaaaagatc ttggacgaac taaatgtttt aaatacagca
     5401 aaagtcaatg tcatacgaga cgggatagaa caaacaatag atatagaaga ccttgtaata
     5461 gatgacattg ttattttaga gactggatat caaattccag cagatgcaga ggtcgtggaa
     5521 gggaaactgc aagttaatga ggctttattg acaggcgaag cagatgaaat aaccaaagaa
     5581 ataggtgatc cattaatgtc cggcagtttc attgtttccg gaaaagctta cgcccgatta
     5641 gacaaagttg gagcagattc gtatatttcc cagctgacag tcaaagccaa gacaatggga
     5701 gaaggagaac agtcggagat gattgcttct cttaatcaat tgatcaaatg gattgggatc
     5761 atcattattc ctatcggtat cctgcttttt tctcaaagtt acttttttaa tggaaacacg
     5821 atcaaagaaa gtgtcgttgc aatggaagca gcattgatcg gtatgatacc tgaaggactg
     5881 tacttgctga cgacgattgc attagcatta agtgcgacta aactagcaaa gcaaagagtt
     5941 cttttacaca atatgaaaag cattgagaca ttggcaaggg taaatgtcct ttgtgtagat
     6001 aaaacaggaa cgatcacaga aaataaaatg tctgttcaaa aaatgatcgt ctctaaaaaa
     6061 caagaaataa tcagtcagtc agatcaattg gaagcatgca ttgctgacta tgcaaaagcg
     6121 atggcaggag acaatgcaac aatggaagct gttaaagcat attttacaaa aaccactgat
     6181 caatcctatc accatgttat tcctttttct tctgtccaaa aattcagcag tgtagcgttg
     6241 gcagacaagg tgtatgtttt aggagcgccc gaaatggttt tgcgcgatca attgccggaa
     6301 tatgcggaag agtttgtttc tttcgctgaa aaaggctatc gcgtgttggt ctttggtgtg
     6361 tacgatggga ttcttgaaga accagtattg acagaagcag tcacaccatt aggctatatt
     6421 ctgattgcta atcctatcag aaaagaagca agagccacat ttgattactt caaaaaacag
     6481 caagtagcca tcaaagtgat ttctggagat aatccgctca ccgtatccaa tgtagctgtc
     6541 caagcaggga tttcacatgc tgatcagtac gttgatgcat ctaaattatc agaagaagaa
     6601 tatgaagcag ctgtcgaaaa atatacagtt tttggacgcg tcaagccaga acaaaagaaa
     6661 atcttcgttc aattgataaa gcgtaacaat acagtagcta tgacaggtga tggcgtgaat
     6721 gatattctag ccatgaaaga agcagactgc agtatagcga tggcttccgg aaatgaagcc
     6781 gccatgcagg cctcacaagt ggtcttactt gattctgact tttcacgcat gccagaagtt
     6841 gttgcagaag gacggcgcgt tgtgaacaat attgagcgat cagccagctt gtttttagtg
     6901 aagaatattt tctccttttt gctctcactg ttttcagtga tctttgctct gacctatcct
     6961 ttagaaccat cacaaatcac attgattagt ttgtttacta tcggacttcc atctttcttg
     7021 cttgctctag aagaaaacaa aaagcgaatc agagggaaat tcatcatgaa tgtgatggaa
     7081 aaagctgtgc caggtggact aacagatatg atcgtagtcg gtgcgttggt gatctgcggg
     7141 gtgactttga atctaaataa aacagatgtt tctacagcat ctaccatgct tttgattgca
     7201 gtagggttcc tcgttctcta taaaatctgt tcaccgctga ataaattcag aagtcagatc
     7261 attcttttct gtgctagcgg taactttttt tcggtcatct ttttgcataa gctgttttca
     7321 atcaccggta tctcagctgt atcacttttg ctgttgagca ttctattttt ctcggcagat
     7381 tcgattttta ggcatttgac cacattggta gaatatttgt ttgaacgtaa aaatgaaaaa
     7441 gagcaaaagc cgtttagtcg cgtagctaaa aaatttctat cgatatttaa acgagaaaat
     7501 tgacgaaaag tatctcaaaa aagagtaaaa aagaacaacc actcgtgtat ctttcgaagc
     7561 aggatgagtc atccaacttt aaaacgcacg agcagttgct cttttttaca ttttcggaat
     7621 ggtaacggtg aaagtcgaac ctgttccaag atgactgtct acagtaatcg taccattaag
     7681 gagttgcacg tagtctttta caatcgctaa ccctaatccg gtcccgcctg aatgtcggct
     7741 tcgcgccttg tctacgcggt aaaaacgctc aaagatccgt tcctgatcct caatgtcgat
     7801 acctatacca aagtcttgga cagaaaacga tagatcatct gttgcctgat agcggataat
     7861 aatctcgctg tcgctttttg aatattggac agcattttca atcagatttt tgataattgg
     7921 ataaaggata tctgttttag tagtaaatgg ttcgttcttt ccaatcaatc gaatcgtcaa
     7981 ccgtttcttt tcgatgattg gctgataatt ttgaacgatt tgctggaaat aaggttctgt
     8041 ataaattgtc tggatctcgt aagaattctc gccatctttt gacagctgga tgatttcccg
     8101 aatcagttta tctaaccgga tcgcatcttt ttgcatgatc tcaagaaaag aagtcagagt
     8161 ttgcgggtca tcttttgcac catccagtaa agtctcggta aaaccaatca gtgaagtcac
     8221 aggagttttt aattcgtgag agacgttgcc aacgaaatcc ttttgtagct tttctaaacg
     8281 tcgcactttc gttaagtcat acgccacccc tagaatctgt ccggtacctt ctgaattagt
     8341 aaagtagcgc aaactcaagt ctaatgtctg ccgaccttct gtaagggtga tctcctgatg
     8401 gatcagcgga tgctcaggag tcacttgatg aatcagctga atgagttttg gctcttgaat
     8461 gacttctgtg taccgctgtt caggttgata gcttaccacg tttaaatgta gctgcatctt
     8521 tggattaagc agtagtaaac gagaatcagc atcatcaatc aggaatacgc caatcatcag
     8581 ttcgttcagt aacgtatata attgctcttc tgtggtagta cacgcccgat acatcttact
     8641 catttgttcg ctcaacgcat taatcgtctg atacaattct tcccattgat cgggtgtttg
     8701 catgattact tccgcctgat taggattttg caccattttt tttagtacgg gcagcacggt
     8761 tttcaatggc tggttttttt gatagatcag ataatagatc attcctgcaa gaacaaggaa
     8821 gaaagacaga aagaagaaaa agacccaacg gcgaaaactc tcagttctcg gtaaaaaaac
     8881 gcttgtcggt tctgcgatac gtaaaatggc ttctaactgg ccactttttt tgactggtaa
     8941 tgcgacatat aaaagatcct cattcaaagt agtacttttg cgcaaagcgc tacctaggtt
     9001 cccaccattc aatacggctt tgatttcggg acgggagttt cgctgttcat tcaatgtttc
     9061 attgtttgtg tcaaataaga tctttccagt agcatccatt aaagtaatcc gttcgttcga
     9121 ctgatggacg aattcttcca aagcagtttt gttttgcgct gcttggaggt tttcgacatc
     9181 taattgccgg atcagcagtt gtgctttttt tgtcaggtag ctttcttgct gttccacgat
     9241 ctgctgttga aaataatggg aaatcaattg ccagccacca aaaaacaaac taactaaaag
     9301 aataagaaag aagagacgtt cataaatttt tttccatttc atcgtttggg ttcctgaaac
     9361 ttatagccaa atcctctaat agtcaccaag tactttgggt gttttggatc tcgctcgatt
     9421 ttttctcgca aatgactaac gtgtacatct acgattcggc tttgtcctgc gaagtcaaaa
     9481 ttccaaatgc ggtctagcaa tgtgtctcgg tcgatcacac ggtcttttcg tttcatgaaa
     9541 taaacaagta attcaaattc tttcggtgtt agttcaatca gatggtcgtc tacggtcact
     9601 tgataattgg tcaaatcagc agtgatttgt ccaatcgata gatattcagg ctcagcttca
     9661 tcttttgatt gcttacgagg ttcaattcgt ctgaatattg ctttcatacg ggctaatact
     9721 tcgcgaggac taaatggttt ggtcaaatag tcgtcagcac ctatttctag gccaatgatc
     9781 cgatccactt ggtcatcttt agccgtcagc atcaaaatcg gtgtttcatt tttttcttga
     9841 cgcagccgct gagtgatctc catgccatcc atcgttggaa gcatcacatc taaaatgatg
     9901 aaatcaaatg attgttctaa tgctaattct aatccttcgc ctccatctgt ggcactggtt
     9961 accttgtagc cttctttttc caaattgaac gtcaagagcg ttacaataga aggttcatca
    10021 tcaacaacta gtattttttt cattcaaatc tcctttaaat aggtttcatt ctgtttatat
    10081 tttagcatag aatcagtaca aagggctatt ttacaaaaga aataaatggt ataataggaa
    10141 ggcactttgc tgaatagaaa ggaagagcag tcaatgattg gaatcagtgc atgtttaggc
    10201 ggtctggcct gtcgctatga tggacagaaa aaagaagtac cagaattgat ggaactagtt
    10261 gaaactggag aagctgtcat ggtctgtcca gaagtgatcg gcggattacc aattcctaga
    10321 gatcccgctg aaatcattgg tggaaatgga ttcgatgtgt ggaaagatca agcaaaagta
    10381 tggacgacaa gcggcgaaga tgtaacggaa gcgtacaaaa atggcgcgat caaagcctat
    10441 caaaaattgc aggaaaaaca cattgatcta gtcatcatga aggaaaaaag cccttcatgc
    10501 ggaacccatt cgatttatga cggaagtttt tccggaataa aaaaaaatgg tgccggagtg
    10561 gctactgctt attttattca gcaaaaaatg actgtgctct ctgatgaaga atggctgaaa
    10621 actagaggtg agcagaatgg aaatcgaaaa gaccaatcga atgaacgcgc tctttgaatt
    10681 ttattcgacc ttattgacag aaaaacaaat gaactacatg gaattatatt atgcagatga
    10741 tttttcatta ggtgagatcg cggaagaata cgaagtaagc cgacaagcag tatatgacaa
    10801 tatcaagcgg acaagcaaga tcttggagga ttatgaaaaa aaactccatc ttttttctaa
    10861 ttatattgtt cgagaacagg tattggaaaa tatgactgca tatattacag ataagtaccc
    10921 ggaagataaa aaactacttg aatatgtaca gcaaattcaa gcaatcgaag aataaataaa
    10981 aggaatgaag aaaatatggc atttgaaagc ttaactgaac gtctccagca ggcgatggga
    11041 aaaataagaa aaaaaggaaa agtatcagaa gctgacgtaa aagaaatgat gcgggaaatc
    11101 cgccttgcct tactagaagc cgatgttaac ttgcaagtag tcaaagattt tactaagcgc
    11161 gtacgcgaac gtgcggtagg ggcagaagtg ctggaaagtt tatcacctgc acaacaaatc
    11221 gtaaaaatcg ttgatgaaga attgacgatc acacttggtt ctgaaacagc agagttaaat
    11281 aaatcaccaa aaataccaac tgttatcatg atggctggtc ttcaaggggc aggtaaaacg
    11341 acctttactg gtaaattagc caattattta aagaaaaatg aaaatgctcg tccgctccta
    11401 atcgcagggg acgtatatcg accagctgcg atcgaccaat tgaaagtatt aggtcaacag
    11461 ctagacgttc ctgtatttga tatgggaact gaagtcagcc cggtagagat cgtccgtcaa
    11521 gggatggagt tagcaaaaga aaagaaaaac gactatgtat tgatcgatac ggcaggacgt
    11581 ctgcatatcg atgaaacatt gatggatgag ttgaaacaaa tcaaagaatt gacgcaacca
    11641 aatgagattt tgcttgttgt cgatgccatg actggtcaag atgccgtcaa cgtagctgat
    11701 agcttcaacc agcaattagg tattacaggg gtcgtcatca ctaaacttga tggggatact
    11761 cgaggcgggg cagcactatc gatccgctca gtgacaggcg caccaatcaa attcatcggt
    11821 tctggtgaaa aattgactga cttagaagta ttccacccag atcgtatggc tagccgtatc
    11881 cttggtatgg gggatatgct gacattgatt gaaaaagcgc agcaagacta cgacgagaaa
    11941 aaagcggaag aacttgcaca gaaaatgcgg gaaaattcat tcgatttcaa tgactttatt
    12001 gaacagcttg atcaagtgat gggtatgggc ccactggaag atctaatcaa aatgatccca
    12061 ggaatgaatc aagtccctgg aatcgaaaac gtcaaggtag atccaaaaga tgttgaacgc
    12121 aaaaaagcga tggtttattc tatgacgcca gcagaaagag caaatcctga tctgttgaac
    12181 ccaagccgtc gtcgccgaat tgctgccggt tctggaaaca gtgttgttga agtgaatcga
    12241 atgatcaaac aattcaagga atcaagaaaa atgatgcagc aaatgtccaa aggggatatg
    12301 aacatcccag gtatggacca aatgtttggt tcaggcgtca aaggaaaact aggcaaaatg
    12361 gcgatgaatc gaatgatcaa gaaaaataag aagaaaaaga aaaaacggaa ataagatgaa
    12421 aaaaacagct tcggtacaga ctgaaaatgt ctactgccgg agcttttcta tcagcaattt
    12481 ttattcttta ctaatacaat tactctaaac gaagaggtgg cacagatgag atgtccgtgg
    12541 agtgaacaaa cagaaagtat gcggcaatat catgatactg tctggggtgt tcctgaatat
    12601 gatgaccaaa agctgttccg aaaattgatg cttgatatta atcaagccgg attgagctgg
    12661 cagacgattt taaataaaag tgaagcattc gatgaagcat atgatcgatt cgatatcaaa
    12721 aaagtcgcaa attatggaga aacaaaaatc agtgaactaa tggaaaacaa aggaattatt
    12781 cgtaaccgaa gaaaaatcga agcagccgtc caaaacgcca aagcagtctt gaaaattcaa
    12841 gaagaaatcg gcagtttttc agagtatctt tggtcgtttt ctaatggaaa aatcgttgat
    12901 tcttcttatc attcacaaga agaagtccca acaacaagtc ctctttcaga tgaattagca
    12961 aaagatctaa agaaaagagg atttaaattt atcggaagca caacaatcta tgctttttta
    13021 gaagctgtcg ggatcatcaa tgatcacctt gacagttgtt tccgcaaaga agaggtcgta
    13081 cgtatcggca gaaagggcga ataaagcatg cgaaaaatca tattttatgg tgctataagt
    13141 ttagatggct atttggcaac gaaaacggat gatcttcaat ggctttttga cactccaaca
    13201 ggtaagcaaa ctacttatga agctttttat gaaacaatcg atacgaccat catgggcaga
    13261 aaaacttatc aggaagcaaa aaaatatttg gagaccgaag caatttatcc agagaaaaag
    13321 aatttcgttt tttccaccca cgcacaatta caattgccag atgctgaggt cgtaaacgaa
    13381 gatcctgttg cttttctaaa aaatctacaa atgacagaag gaaaagatat ctgggttgtg
    13441 ggtggcggtc gattgatcaa accgctattg gaacatcaaa tgattgatga atggtatatc
    13501 cagattaccc ctgtattatt aggggacggg attcctttat tccaaaaagg aacatatgaa
    13561 gcgcgttttg aaatgctaga cacgacacga ttcggtgaat ttatcgaatt gcattatgta
    13621 cgaaagtaga agtaaataaa ataaaaaagg caactatatg cagaaatgtc ctgtacatag
    13681 ttgccttttt tattaccgtt atgcttttta ctctgttaat ttgttagaaa aatctagata
    13741 cgcatctaaa atcgtttgga agaaacttaa tgttccttca acgatattac cattttcatc
    13801 aattagattc gcaacgtttc cgatatatgc ttccggttgc tgcaaggttg gcacattcaa
    13861 aaagacaagt gattggcgta gatggtggtt tgcgccaaag ccgccgatcc cacctggtga
    13921 tgcagtaacg actaaacctg gtttatgatc ccaaacactg tgtccataag gtcttgagcc
    13981 tacatctaaa gcatttttca atactgctgg cacagaacgg ttgtattcag gagtaacgaa
    14041 aacgacaccg tctagtcccc tcacttcctc acgaaaacga gtccattcag caggagcctg
    14101 ttctggagtt tcgagatcct cattgtaaaa tggcagatca tcgattttta caaatacact
    14161 ttcgtaccct tcaggaagca atttcgcaaa agcttctgct acttttttgt tgtaagaatc
    14221 ttttcttaaa ctaccaataa aaaatccaat tttttttgtc atacgatttt tcctccttca
    14281 aactatctat aataatctta caaaagataa gtaactggag caaataaaaa cacttactga
    14341 caaaagctac atagaagata gattacttat ttttaacaaa aaagaagtct aaaatcttct
    14401 attttctaaa aaaaatcgaa tttaatcaag ctgtaaagaa aaaaagcttt acagggtaaa
    14461 aaaaaactgt tacactacat cttgtgaata aaacaatgga ggtggaaata aataatggca
    14521 gttaaaatcc gtttaaaacg tatgggttct aaaaagagtc cgttttaccg tatcgtcgta
    14581 gctgattcac gttctcctcg tgatggacgt ttcatcgaaa ctgtaggtac ttacaatcct
    14641 ttgaaagacc ctgcagaagt agttttaaaa gaagacttag ttcttgactg gttatcaaaa
    14701 ggtgcacaac cttctgacac tgtacgtaac atcctttcaa aagaaggcgt tatgcaaaaa
    14761 caccacgaag ctaaattctc aaagaaataa ggtgaccgga tatgaaagat ttaagcgact
    14821 tagtcttaac aatcgttcgt ccgctagtca cttatcctga tcaagtcaag ttagacgtcg
    14881 tagagtcgaa agacttttac gagtacaatc taacagtggc tcctgaagat attggccgta
    14941 tcattggaaa acaagggcgt gttgctaaag caatccgcac aatcgtttac ggtgttcgga
    15001 tcaatgcacc gaaaaaagtg cgtttgaata tcatcgatga taaggaaaag taaataaacg
    15061 tgctgtactc gaattttcga gtgcagcttt tttgtttttt ttattaattg aagaaatgat
    15121 tatttggcaa aacaagcaag aaagcgtatc cttataggag gagggagtga acagccaatg
    15181 agaaagaaaa tgttatttat tccatttgct ctattgttaa gcgcttgtag ttcggttact
    15241 caagaatcag acaatacaca tctatcaagc agtatatctg aaactgttac cagtccagaa
    15301 aaaagtagta ccaaaacggc ttccaccact gaacaaacaa tggctacctc aaataatgag
    15361 aaaaagactg ctttagacca gttgaaagaa caacagccaa atgttcccat gcctctagat
    15421 gtaccagtct cttcaggcta tctcaatatc gcagcgacac atacaaagca aggttattct
    15481 attttatatt acagaacaga tcgtcccctc gggttgaatg ctgacgaatt gaatcaagaa
    15541 acgcctattg caacctatct atatcaatat gggtttgctt ctagtcaaga aacaatccaa
    15601 gttttgcagc catttgagat cgacacaaat ggtcagcaag tggacttagg ttctcgaatc
    15661 acagggtatc agcaaggtgc agcaggttca agttttcttg aatggcaaga aggaaactgg
    15721 cgtattcgaa tccgtgggaa taatatagaa ggccaagatc ccttactatt agctaaagaa
    15781 atcgtagctt atttggaaga aaacagtcta ccagcacctg aacaatttgg taaaattact
    15841 gttgatatgg gtgatacgac gaatcgagcc gtagaagtaa gctggcaaga accgaaaaat
    15901 gcctatacga tcactcatca agatcctatg tctgcgttga agatggcagt ttctatgaag
    15961 aggttgtgaa atcagcgcgc tgatctgagc ccatcattta actccaagtc acaggaagta
    16021 atcaaaaata gcacgccgca tttttgcata attttggctt aataccttaa aagaaaacta
    16081 gaattgcctc ttcacacatg ctaaaataaa acagacaaaa aagaaatggg gatataagtt
    16141 gactgaatac ttgaatgtag gaaaaatcgt aaacacacaa ggtatcaaag gcgaagtccg
    16201 tgttatctca acaacggatt tcccagaaga acgttacaaa aaaggaacgg tgctaacact
    16261 attccaagac ggaaaagcac ctgtggaatt aacagtcaaa agtcatcgca aacataaaaa
    16321 ttttgactta ttgagttttg aaggacatcc ttctatcaat gatgtggaaa aatatcggga
    16381 tggcatatta aaagtgtcaa aagacaactc tgtagatcta gctgaaaatg aattctatta
    16441 ccatgagatc atcggtctgc atgtattaga agatggaact gagttaggta aagtaaaaga
    16501 aatcttgtca ccaggtgcca atgatgtttg ggtcgttcaa cgtccgaaaa aagctgatat
    16561 attgcttcca tatatcgctt ctgtcgtcaa agaagtcgat ttagaagctg gagtggtccg
    16621 tgtagaaatt ccagaaggac tgattgacga tgaagattga tgtattgact ttatttccaa
    16681 gaatgtttga aggtcctatg ggggaatcca tcatcggtaa ggctgtagat aaaggattat
    16741 tagatatcaa tatctctaac ttccgcgatt attcagataa caaacatcag acagtagatg
    16801 attatccata cggcggtgga gcaggtatgc tgctcaaagt ccagcccatc tatgataaca
    16861 tccaagccat tgaaaaagct gcaccggatg tcaaaaaaag agtaattctt cttgacccag
    16921 ctggtaaacg attcgatcag aaaatggcag aagaattttc acaagaagaa cacttagtat
    16981 tcatttgtgg tcattatgag ggatatgatg aacgtatccg cacattagtg acagatgaag
    17041 tttccttagg agattatgtc cttactgggg gagaattagg tgcgatggtc atgatcgatg
    17101 caacagttcg gcttttgcca gatgtgttag ggaatcagac ttcagctcaa acggattctt
    17161 attcaaccgg tttattggaa catccccaat atactcgccc ggcagaatat aaagggatga
    17221 aagtaccaga agtattgaca aatggaaatc ataaattgat tgaagaatgg caattaaaag
    17281 aatcactaag aaggacctat caacgtcggc ctgatctttt ggaaacactt gaatggacac
    17341 ctcaaatgac gaaattttta gaagaaatca agaaagaaga acaagaaaaa acagcagatg
    17401 aagccaagtg actttttcta aaatcttctt tacagtaggt tgataatatg ttagtatttt
    17461 atggtgagtg caaaagcact taactattac gatattccgc tgtgagaaaa gctcataaga
    17521 atatttggaa taaggagaag aaaaccatga atccattaat cgaagaatta acaaaagaac
    17581 aattacgttc tgacatccca gcgttccgcc ctggtgacac tgtgcgtgtt cacgcaaaag
    17641 ttgttgaagg tactcgtgaa cgtatccagt tatttgaagg tgttgttatc aaacgccgcg
    17701 gtgctggaat cagcgaaact tatacagtac gtaaagtttc taacggagtg ggcgttgaac
    17761 gtacattccc attgcataca ccacgtgttg caaaaatcga agtagttcgc tacggtaaag
    17821 tacgtcgtgc aaaattgtac tac
//
LOCUS       DACDIH010000053        17586 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDIH010000053 DACDIH010000000
VERSION     DACDIH010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.053"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            30..995
                     /locus_tag="IV281_001925"
     CDS             30..995
                     /locus_tag="IV281_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8780908.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(1094..2266)
                     /locus_tag="IV281_001926"
     CDS             complement(1094..2266)
                     /locus_tag="IV281_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8780909.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(2253..3398)
                     /locus_tag="IV281_001927"
     CDS             complement(2253..3398)
                     /locus_tag="IV281_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8780910.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(3412..4344)
                     /locus_tag="IV281_001928"
     CDS             complement(3412..4344)
                     /locus_tag="IV281_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8780911.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            4461..5540
                     /locus_tag="IV281_001929"
     CDS             4461..5540
                     /locus_tag="IV281_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8780912.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            5537..6166
                     /locus_tag="IV281_001930"
     CDS             5537..6166
                     /locus_tag="IV281_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8780913.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(6276..6503)
                     /locus_tag="IV281_001931"
     CDS             complement(6276..6503)
                     /locus_tag="IV281_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8780914.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(6606..9194)
                     /locus_tag="IV281_001932"
     CDS             complement(6606..9194)
                     /locus_tag="IV281_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP8780915.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(9214..9873)
                     /locus_tag="IV281_001933"
     CDS             complement(9214..9873)
                     /locus_tag="IV281_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8780916.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(9922..10425)
                     /gene="trmL"
                     /locus_tag="IV281_001934"
     CDS             complement(9922..10425)
                     /gene="trmL"
                     /locus_tag="IV281_001934"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP8780917.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(10646..11512)
                     /locus_tag="IV281_001935"
     CDS             complement(10646..11512)
                     /locus_tag="IV281_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8780918.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            11621..12262
                     /locus_tag="IV281_001936"
     CDS             11621..12262
                     /locus_tag="IV281_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP8780919.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(12324..13685)
                     /gene="mgtE"
                     /locus_tag="IV281_001937"
     CDS             complement(12324..13685)
                     /gene="mgtE"
                     /locus_tag="IV281_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP8780920.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPLKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(13703..14602)
                     /locus_tag="IV281_001938"
     CDS             complement(13703..14602)
                     /locus_tag="IV281_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8780921.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(14604..15401)
                     /locus_tag="IV281_001939"
     CDS             complement(14604..15401)
                     /locus_tag="IV281_001939"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP8780922.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(15379..16083)
                     /locus_tag="IV281_001940"
     CDS             complement(15379..16083)
                     /locus_tag="IV281_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP8780923.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            16214..16789
                     /locus_tag="IV281_001941"
     CDS             16214..16789
                     /locus_tag="IV281_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP8780924.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            16907..17584
                     /locus_tag="IV281_001942"
     CDS             16907..17584
                     /locus_tag="IV281_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8780925.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         5196 a         3783 c         3133 g         5474 t
ORIGIN      
        1 cttgttttat tgaatagcag gaggaaatca tgaaattcca ttatcatata ttgatcaatc
       61 catcggcagg cagcggaaat ggctataaag tagcagaaag aattttaccg gtattaaaaa
      121 ataagcatat tgactataca cttcattata gtgaatacaa aggacacgat gctgagattg
      181 ctgaaacttt ggcaaaagaa acattgattc cttggataga agaagaacag aaaacaatcg
      241 atactttccc ccttttgatc atcgtaggag gtgacggcac cttacatcag gtacttgata
      301 ctttctatca gatggaagta gaattcccag ttgcttatat tccagcaggt tctggaaatg
      361 attttgctcg aggggcaggc cttccaaaaa atcccaaaaa aggacttcag ctgattttag
      421 cagctcaatc accagaaaaa gtccacataa tggcttatga agaaaaaata agtgaaaaaa
      481 aaggaatagc tgttaataat ttcggtatag gattagatgc agctatcgtc catgcgacaa
      541 atcattcttc cacaaaaaag cgattgaaca aatacaactt aggatcattt tcgtatttat
      601 tctctattct ccgtgcttta ttcactcaaa aaggatttcc aattctggta gatgcaggag
      661 gaaaacaatt aagtttttca aacgcatttt tatgtacagc aacgaagcat cctttttttg
      721 gcggaggaat cgcgattgtt ccaactgctg atgcgtctaa accagtcatt gattttgtcg
      781 tggtcgaacg aatcaatttc tttaaaatct tgtggctcat tcttttactt atacagaaaa
      841 aacaattgaa atcgaaatat ttccatcact acacaacaaa cagattacgt atcgtttcaa
      901 caacaccgca acatggtcaa gaagatggag aagaaatgga aaaacgacct tttgatatca
      961 caataaccac aactaaacag ttgttttggt gttaataaaa gaggttttgt cagaagctac
     1021 atctggaaca ccgtttattc ggaaaaaagg actgtgacac gacttttgtc acagtccttt
     1081 ttttactatg cagttaattc tttgcccaat tgggttgcca tcgatagttt ctttctttac
     1141 ccaaaataaa ttggatcatt gaaaacaccg cagcaaaagc aactaagaca caaccattga
     1201 accaaaaaga ttctgcaaat gtttgattgt aactgagtgt ttcactgatc aagttattat
     1261 tttgaacaaa ccaatatgtt ggtgtgaatg ccgctatttt gctaaccatt tctggcaagt
     1321 attctgctgg tacaaacacc ccaccgataa agcaactgcc tgtgataaaa atatctttga
     1381 tcccaccgct ggtttctgag ttttttacca atgaagtgat acaagccgaa aagctaatga
     1441 tcggcaataa aaacaagaaa gtatttaaaa tatgatacca taccgttata tcccacgagg
     1501 tgtggatcac taacagaacc attagaagta agaagccact gaacaacagc agcgagtaac
     1561 taatcaaact aaaggttact ttgcgattca agtttcgttt ggtaatgggc gcactctggt
     1621 ttcgtttact gatctctttg cgattaaacg ctagattgat gacgctgaaa ccgctaaaaa
     1681 tggatagaaa caacccataa gacaagaaat taaaggttgt gcccgtcaac agctttttca
     1741 tccgctgact atatcccgca cttagctcaa cttctccagt aaccgagagt gccatttgtg
     1801 tctgttccat agctgcatcg attgttttat cttcttcttg aaacagaatc agtgtttgca
     1861 aaaaggtgtt cacttgctgt gtaacaactg ttttagaaaa ggcatctggg cgactctgta
     1921 tcttgatcgt cgccatcttt cctacttcga cttgttcggt aaagtctgca ggcagatgta
     1981 agataatgtt tacacgatta aaataaagag catcatcgat ttctttttgt gaggttccat
     2041 ccaatgtaac gactgtttcc ttgtcattca aatatttacg aaggccatcg actacaggcg
     2101 actcttcttc actaatcaag gcgattttcg catcttgcag agaggtattt tcaacatcaa
     2161 tattttgcgc aaaaaagaat gtggcaacaa cagtgataat tacacctatc agtaaattcg
     2221 atttatttgc tttaagcaat gtccaaatcg ttttataaag cgtcataacg cacccctctt
     2281 tccatccaag catttcctaa cgcaaaaagg aaaataaatc cgatcaacca tagaagattg
     2341 gtataaaaac tagccaaatc agtgtagaaa taaagtttat acaaactttc gctgatcaag
     2401 ttgacgagat ttaattgacc aacaatcgga atatgaagat caatccaata cttcacttgt
     2461 tccgacccca tcataccagc aaaaaagctc atcgcaatcg tcactgaaat caagatgcta
     2521 tccttttgca ccagattcat ttttggtatc aaattaccaa ccaatgtacc taacaaaatc
     2581 gcacacaacg accccagagc ggtaacaatc atcaaccatt gccaacgttc accaaagtct
     2641 acttgataga taaaacgaaa gacagctaac ataatataaa tctctgtcaa aacaatcgta
     2701 aagcttgctg ccaaattagc gattgaaact aacgattgct ttctaggaat caaggacaag
     2761 cgaataccat ttgacgattg atttgcttgc tgatcctgta cattgcggac cccccacatc
     2821 gtcccgtaga gaatcgccat tccaatcaga gtgaagaaat aaaaactttt gaaactcata
     2881 ttacgacctg catgaacttc ttgaaaagat acttcttgtt caaaaacagt catgatgggc
     2941 gcgagatcaa cgttctccac ttctgccact ttactcgaaa ttataccaat acgctgtaaa
     3001 aattgatcca tgacgttttt catcaacgtc tgactcattc ctgcttgacc cacatggagc
     3061 gaaacagatg aaccatcaat gagataaaaa ccagccaaat catctgcatt tagcttttca
     3121 ttcgcttcta cttccgatag ttcctgtact tcaaataatg gtttatcatt gacttcgact
     3181 tgttctaaga ctgtggtaaa attttccgaa agcgctgttt gatccgcttt gattacaatt
     3241 ccagtattaa tcgtttcaag attgccacct tgatcaatct cgccaaaggc cgccataaac
     3301 atcaatccta aaagaattgg aaaggccaat gtccaaaaca acatggattt atttcgcagc
     3361 agtatttttg tgcgatattt ataaaaatgc caaaacatta tttcccctcc tctagtctct
     3421 caaatccttg ccagtaatct ctaaaaagac atcattcaag gacgcttctt tggttgctat
     3481 ttttccgtaa ctgatctttt cttcttgtaa gattgtaaga atcggcaaca atactgtatt
     3541 tagttcagta gttgttactt ggagctgacc ttttttataa gcgattccat cagaagctat
     3601 tgttcgctga atcgctgaca acacattttc tgggagtaat gggatgtcga tcgtcaaacg
     3661 atccgccgtc cgtaccatat cttttagaga ttctttggtt cctttagcaa tcacctgtcc
     3721 ttgatccatg atcacgatct ggtcgcaaag aatttctact tcttccatgt aatgtgttgt
     3781 atagacaatg gttgctcctt gtcgattcag ctccttgata ctttctaaaa ttttattccg
     3841 actttggggg tctacagcta ctgtcggctc atctaaaaaa attaaccgcg gcttgtgggc
     3901 aatcccacat gcgatgttca accgacgttt caagccacca ctaagttctc ctggacgaaa
     3961 cgaaacatat ttttctaatc ccactaattg aatcacctct tcaatcaagg ctttcctttt
     4021 ctctttttcg gaaacatata aaccgcaaaa ataatcgata ttttcttgca cacttagttc
     4081 gtcaaagacc gcaacgtctt gaggaacgac cccaatcatt gccttgacag cataattttc
     4141 aggagtcatt gttgtcccga acaatttaat ggttcctttg tcataattta acaaagaaag
     4201 catacagttg atggcagtcg atttcccact gccgtttgga cctaacagac ctaagacttc
     4261 tccttcttgt acttgcagat caaaatgatt caatgctaca aaatctccat agcgtttgac
     4321 taatttatca atttcgataa tcatcatgaa aaactccttc caaatttctt gttgatctga
     4381 ctataataaa gtgcaagaaa agaaactagt gacgattggc taaaccacac atgacatttg
     4441 tcacaatgaa ggagtctgct atgcataaat ctcggctact caacgaaact agctttctac
     4501 tggttatctc attgcttttg ctggtaagtc aaggattcgt tgctatgcaa gttatctatt
     4561 tattgatcgc cgttttattc actggtgtca gttatttact gcctacaaag ccaaattgga
     4621 ttttccaagt tattctattt cttttatgtg cctttttacc tgatcttgtc ctgtttctgc
     4681 cagcgacgat gcgagtgatc gcccctgaag aaagccacct tgattacttg tttttgttag
     4741 gttttttgtt gatttcgacg gttggtaatc atccccccat caaggtcgcc tttgtcgtaa
     4801 ttttgctgtt tttttccttt tacttacgaa aaaaagatca acgaacgacc gatcttgaga
     4861 aaaagtacat tgtctccaaa gatactcatt gggaaaaaca acaagagtta tcggaacaaa
     4921 ataaagcatt ggccctgtca caagaaacac aattagcctt gcaaattgtg gaagaacgga
     4981 accgaattgc ccgagacatc catgacaatg tcggtcatct gctgtcaagt gcgattttac
     5041 aaattggtgc gcttgaaatg atcaatcaag aaccaaaact aaaagaacca ttagaaaatt
     5101 taagtgccac cgttcacact gggatggatc ggattcgtca aagtgtccat gacctccatg
     5161 agacatcgat ctcttttcaa cactccttag agtttctgat cgctgatttt ccttcttctg
     5221 ttacgattga aggaaacttg tttgaaggat tgaatgaaac gcagcaaaac tgcttcttaa
     5281 tggtcatcaa agaagcctta accaacagta tcaaacatag tcatgccaaa accgttgttt
     5341 tacattttcg aacgttgccg gccttttatc gcttgcaaat cacgaatgat ggcaccagcc
     5401 cacaaaaaaa ttctgctccc ggtattggtt tgaaaacgat gcgtcaacgt gttcaagaac
     5461 aaaagggaca agtccatatt ttccaagaaa accagcaatt tcagttaacg atcattttac
     5521 caagggaggg acaccaatga taacagtagt cattattgat gatgatccat ttgtgacgaa
     5581 atcgttacaa accattttag aaagcacgaa agagatccgt gtgttaggca ttggtcattg
     5641 tgccaaggat gcgctagtct tatacgaaac ccatcgtccc gatgttttgc tgacggacat
     5701 ccgcatgcct aaacaaacgg gcatcgatgc tgccaaagaa attttggcta aatttcccaa
     5761 agccatcctt ttgcttttga cgacattcaa agatgaagaa tacatccgcg aagcattttc
     5821 catcggtgtc aaagggtatt taatcaaaca gaacttgcaa gcgattattc cttctgtcaa
     5881 atccgcttat aatggacagg ccgtatttgg taatgaaatc atcgagactt tcactcaaat
     5941 gatgaagaat aaaccgacga ttgatagtca tgcttatgct tcttttagtg aacgtgagct
     6001 tgcaatcatc aaagaagtag ctgccgggaa aaacaataaa gaaattgcag atgccttgta
     6061 cttgagtgac ggcactgttc gcaattacat cagccaatta ctagaaaagc tagatttacg
     6121 ggatcgcacg caattggcta tctattttta tcagcatttg caataagtct tatctaacac
     6181 aaaaaaacct gaatgatgct ccttcgcatc attcaggttt ttttcacatc ttctattcaa
     6241 ttgtcccgca aaccatttcc tcacgattga aagaatcaag gattcgcagg gctgccagga
     6301 ttcgttggat tgttcggcgt gttaggcgga aaaataggcg ccttgtctaa cgttttttct
     6361 agttttgatt cttcacgatc gtctcgttct acatcaggac tttcattcaa tttgaattct
     6421 ttttccatct catctaactt tttaaagtca ttgccagatt cttttgctaa tttcgctaat
     6481 ttatcttcgg tttctttagc cataaagtaa cgcccccctt ttgatttcag cttagcatat
     6541 cacactgaaa aaacaaaaga aaacgctaac caacgatcat ttttagctat ttctcatctt
     6601 taaaatcaag cattttttgc cggtataaaa tcgtagggag aactgttttc cataccaatc
     6661 atcggatcta ctaactggtt atcgatcact tctgctgtaa gaacgaaatc tttagaaaca
     6721 gcaggttcta cctcttcttt cttttcttct ccaacaaatg aagaaaacag atctggttcc
     6781 actgtttcca ttacttggtt tttcgtttca ttcgtcccac tgtcatcttc tacaggtagc
     6841 ttttctttta caacccgtgg ctcttttgta tagttcccat cttcaaatgg atcatttccg
     6901 accaaagaat cttcataagc tgctagtttg gtccggagag tgatattcat tttatcatta
     6961 tccatgatcc ttgatttcaa cgtagtcatc cggcttgctg attcattcgc tacacaagtg
     7021 acataggctt gctcttcccc taacaaaatc aacagttcct tactgcgagt cactgccgtg
     7081 tagagcaaat tacgctgaag catccgctga tattgatgga ccatgggcaa aataaccatc
     7141 ttgaattcac tgccttgtga tttatggatt gagcaacaat aagaaagagt gattttattc
     7201 cattcgtttc ttttataagt gacctcattt gcatcaaact ggataactaa ttcatccact
     7261 ttatcttcag aatcttttgc caagatgata cccgtgattt ctcccatatc tccattgaat
     7321 acattcaatt cgggagtatt gactaattgg aggactttgt ctcctatccg ataaacggtg
     7381 tcgttccaat gtacttcctt ttttccaggt ttccctggat tgaatatctc ctgcatcatt
     7441 ttatttaacg catcgatccc tgcaggtccc cgatacattg gtgctaatac ttggatatct
     7501 tgtgcacgga atcccttatt tttcgctttt tccacaattt tagagatcaa gggttcaatt
     7561 tgataagcat cacttcggaa ataggaacga tctttttgat tctttgtgaa atcttttggc
     7621 agacgtcctt ctttgatctc atgggcaaga ggaataatac tagaaccatc accttgccga
     7681 taaatctgat tgagttccat tttgggaatt tgttcgattt ccaaaagatc atggagtact
     7741 tgaccaggac cgacagaggg aagctgatct ttgtctccta ctaagataac ttgcatattc
     7801 gtcggaatcg ctttgaataa tgtgttagcc agccatgtat cgaccataga catctcatca
     7861 acaatcaaaa gtccgccttc taattctttc gtactgactg aaggtgtttt ttcccgtcca
     7921 ttcagcccca gaagacgatg gatcgtactg gcaggcagac ctgtcgtttc gttcattcgt
     7981 ttagctgctc ggccagttgg tgctgcaagt agaataggaa aaatttcttg ggtatatttt
     8041 gaaggatcca attccagtcc gttcaactct gcaaaaagat tgacgattcc gttgatcaca
     8101 gtagttttcc ccgtacctgg tcctcctgtt aaaataaata gaggggaacg gattgcttca
     8161 gcgatagctt gttcttgcga aggtccatac tgtatgccga atattttttc gatacgccga
     8221 atattcttct gcacttcttt ttcaggataa tggatttctt ttttacgact gagaaggcgc
     8281 tggatcgaat taccgatccc ccattcagaa aagtacaaac tgttttcgta aagattagtc
     8341 tcttcttgtt gtatctttcc ttcttcaaca agttggataa ttacagtagc aacctgatca
     8401 ggctggattt caattgggcg acttgtttct aaaatacgga tcgtttgctg aagcaatgtc
     8461 tccgccggta cgtatgtatc tcctgtttga actgcatgct gaaaaatctg atgcaagata
     8521 gctgctcgaa ttcttctcgg tgaatctgct gcaataccga tttgttcggc tagattatct
     8581 gcacgtttga acccgattcc ttctatgtct tcgactagct ggtacggatt ttcttcgatg
     8641 atatccaaag actcgttctt ataggcctgg tagatactga aagctaactg actaccgaac
     8701 ccgtaacgat tcagtcccat gatcacttga tccatcccgt ggtttagccg aatcgtctca
     8761 gcaagcattt ctctttttgg cttcgtcaaa ccggagattt gttttaatac cgaaggatca
     8821 gcaatgattt tttcgatagc atcttctccc agcaattcta caattttttc tgctgttttc
     8881 ttcccgattc ctgggaattt ttcgctggaa agatacgcta cgatcccatt tgcagttgtt
     8941 ggcttttctt gctcatatct ttcgagttta agctgccttc cgtatttagg atgatccact
     9001 aattctccat aaaaacgata agtctcattt tcttgaatat cgccgaaact tccggtgact
     9061 acaatctctt tttcacggta atcagctgtg gtttcactga tgtgtaccaa aagcactttg
     9121 taaaaattac taggattttg aaaaaaaatc gcggcaactg taccaacgaa ataaggcatt
     9181 tcttctggct gcatcggaag ccgcctcctt cttctatagt aaaggttctg gtgctgtttc
     9241 agttcctaaa aaatctgttt ggttccatac tttcagttca tatggcaata atctgcaagg
     9301 ctcttcagtc tctaaaatcg tcaaactact attgaacaat ccacccattt cacgcaactc
     9361 acttagctct tttcctgtca tccattgaat ggctgcagtt aaagaagctc catgtcctac
     9421 gaataaaagt gggccatctt tatgtcgggc aaccgcatct gctacaacag tttcgatacg
     9481 tgtgattgcc gattcaatcg tttctccgtg aaaaggagaa ggatcataca gatctaaatg
     9541 attacgcaaa ttcggcagat tatccggata tttctggtac atttcatcga tcgactgacc
     9601 ttctaaagta cccagcccta gttctcttag cttatctgta taaaaaatct ctaccggacg
     9661 agcaagctct ttgttgatac cttctgctgt atcacgtgct cgctttgctg tactggaata
     9721 aattgcttta aaaggaacat attttacctt tttcccaaat gctttgattt cttcgtaact
     9781 ttgtggcaaa agcggagaat cgccattgct cccttgaaaa cgtctttcct gattccattg
     9841 tgtcttccca tgtcgggtaa aatatagttc catacgcagc aaactcctgt ctttctttta
     9901 ttttactcta taataaactg tctaatccag cttgtcattt tcatagtgat gcgtcaattc
     9961 taaattaggg aatccttgct ggcgaagtgc ttcatacact atcattgcag ctgtattcga
    10021 taaattcaat gagcggacat gctcatcatt catagggatg cgtaaacact tttcttcgtt
    10081 ttcacgcata aatgtttctg gcaaaccggt tgtttctttt ccaaataaaa agaagtgctc
    10141 ttctccatca gcaaaatttt cgtcactata tactttatgg gcaaattttg agatcaaatg
    10201 tagtttacga ttacctaaaa atgttaaaaa agcttctaaa ttactgtggt aagtaatatt
    10261 tacatcattc caataatcca accctgcccg ttttagatgt ttgtcatcag ttgaaaaacc
    10321 tagtggctcg attaaatgaa gcggagtatt cgtggctgca cacgtacggg caatatttcc
    10381 agtgtttgca gggattaatg gttcaaataa aacgatatga ttcatcagga tcattcctct
    10441 catatttatg ttcgtttcta ttttctgaca atttgatttt tgcataaaaa aaaacgtatc
    10501 aactcggtgt cagtcactgc gagtcgttac gttagtgaag ctcttctcac taacttttat
    10561 cagaacagga acctgataaa aaccaatgaa aaaacaactt gttgctaata taacaccctc
    10621 aacttttttt tgcaacctct actcattaaa aactgctaga aaaacgtttt ctcttagttc
    10681 cttttaataa ttctatgtca attgtaatct tcttcaaagg attcttatat aagtctgctt
    10741 tgattcgctc ttctacctgc cattctaaag gtgacatggc caaaagatct ttttgatttt
    10801 cctctggtat ctcaaattca taggttacac gctgacgttc agttaatttc attgcttcag
    10861 caaacttggc tactacacgt tcattggaat aactctgttt ttcaggttga ttcggataaa
    10921 aagcctgacg tagttccttt aaataatttt cttgtggcac caccttcaac acttctccat
    10981 cttctgagag tacacgttgg aattcttggt aatgagaagg cgaaaagata tttaaaaccg
    11041 cgttgaatgt ttgatcagca aacggcaaat tggtcaaatc agctacacac caaaatgctt
    11101 cggcagattg ttctgtagct gcgtagactc cttcttttga aatatcaaat ccaacagcat
    11161 tttgaatctt tttcaatttt gaaagttcat tcaagaagct accttctccg cagccaacat
    11221 ctaatagcga gacttcttca ggtaaatgct gcgcaatagt ctcaataacg ggctgataca
    11281 tgccagattg gatcattcta cgccttggaa cgaacattcc ttgatcgtat tctgttttaa
    11341 ccgcatggga taaaaaataa agtgttcctt ttttggaaag gtcaaaacgg tgattatttt
    11401 tgcatatcaa gccattatca gactttttta cttcttcttg gcaaaccgga cagcggaaca
    11461 gctgatgatt ttctgccaaa aactggcgtc ctcggtcaat tttttttaac atagttcccc
    11521 ctcatttctg ttttatctta ccgttagatg ctcactttgg caactgctaa aaatatgata
    11581 caatgcctag aagaaagtga aagataaagg agaagattag atgatcaaag aattttgcgc
    11641 tgaaaactac acatctattc ctctagcaat tgctagagga gccgatcgga tcgaattgtg
    11701 tgacaactta gcggtcggtg gcacaacccc gagtactggt gtgatcgaag aaacgttaag
    11761 ttatgctggg gaaaaagata tccccgtaat gacgattatt cgtcctagag ggggcgactt
    11821 cgtctataac gatatcgaat tgaaaatcat gcatactgat ttgattgaag caaagaaatt
    11881 agggacggat ggcgtagtat ttggttgtct tactccttca ggctggctag acgaagaggc
    11941 cttagaacta ttgatcgaaa cagcagaagg gttgcagatc accttccata tggcttttga
    12001 cagtattcct gaagatcggc aattcgaagc aatcgattgg ctggtagaac atggtgttga
    12061 ccgtatcttg actcatggcg gtcctgctga caaaactatt gtagaaaatt tcccacggct
    12121 taaagaattg attgcctata cagatggccg cttaatcatt ttgcctggtg gtggtgtcac
    12181 tgcagagaat gcagcacatg tagctaaaga attgaacgta agcgaagtac acggaaccaa
    12241 aatcgtggat cttcagttat aatcttataa acaaaaaaac atttgcaaca ggcataatgc
    12301 ttattgcaaa tgtttttgtt tttttacata aaatagccca taaataaaga agcaatattg
    12361 aaataaatca gtacactcgt caaatcgctc aaagtcgtga taaagggacc gcttgctact
    12421 gctggatcaa atcctagacg atccattagc atcgggatta aactacctgc aagattcgcc
    12481 actgtgattg caaatagcat agccatccct atgacaaacc ccaagataaa attatgtttc
    12541 caaaaaccta cgattagaaa aatcgtgatc cccgtaacag ctcctgtgac aacgccggtc
    12601 aagacttcag ccaaaacaag tcgaacaaat ccatgatctt tctcatcaga aatcgcaagt
    12661 cgacgaacag ccacagctag cgactgggtt ccagcattac cggcagttcc tgtaatcagc
    12721 gaaatgaaga ctgctaaaat actcgcttca cttaccaagt cttcatagtg actgattaaa
    12781 gaagcggtcg acatacctaa aaacaacaga gtgatcagcc aaggcaaacg tcgagacgct
    12841 gcctttagtg gattttcatt aatttcttcc acattgaccc cggcaagtcc tgaataatcg
    12901 ctagcagctt catcatcaat aacgtcgatg atatcatcga ctgtgacgat tcctaacaaa
    12961 tgatcatcat aatcggtaac aggaagagct aagaagtcat aatctctaaa tgtttgtgcc
    13021 acatcttctt ggtcgtcgcc tacatgaact gaaagcactc gttcgctcat cagatcagaa
    13081 atcattgcgt cgtcatcatt cacgattagg tcgcgcagag aaataacccc tactagctga
    13141 ccttcttgat taacgacata gatgtaatag attgtttcag ctacatctgc ttcgttcttc
    13201 agcacgtaca tcgcagagcg aacagtttga ttggctacga tcgaaacaaa ttcagttgtc
    13261 atgatcgcgc cagccgtttc atcctcgtaa tggagaagtt ccttgatttc actggcatcg
    13321 tctgccgaca tcaaacttaa atatttagca atttgctttt tatccagcgt attaagcaag
    13381 tcaacagcat tatctgtata catctcagaa agcatatcag ctgcatagct aggacgcatt
    13441 tcatctaggt attctttcat atgttcgttg tcttcttcaa tcacatcgaa catatccgct
    13501 aattctttag gcgacagata ggaatagatc agctgcctgt tctcttctgt aagagattgg
    13561 tagaattgcc cctgctcata gatatgcatc tctaaaaaac tgtttctgaa tgcctgaata
    13621 ttattttccc ttaaatcttg agtcaactgg gcaaatcttt cctctaattc ttgattttca
    13681 tccaaaccaa tctcacctcg ccttattctt caaaccgtct cacaactgcc tccatgtctg
    13741 cggctaaagg ctgcttgaac tccagcaact cttctgtaaa tggatggtaa aaacgcagat
    13801 cataacaatg aagggcttgt ctttttattc ccagatccat tcgcccgcca tacatatcat
    13861 caccgagcag ggcacagcca attgcggaaa aatgaactct tatttgatgt gtccttcctg
    13921 tatgcagctg gatatcaact aacgcgattt ctgttagtcg cttctctatc cagtactcag
    13981 tctgtgccga acgtccttca tcagagacca ttcgcttcaa tagcgaagaa taatctcggg
    14041 cgatcggcaa atcgatatgt ccatgtgccg ataaatctga aagccttcca ctgactaagg
    14101 cctgatattt tttcacaaac ttcttttcac gaagttgcat atctaatttt gcgtgcgcaa
    14161 atccatgctt tgcaaaaagc atcaagccag atgtatcccg atctagtcgc gtcaccacat
    14221 ggattacttg gttttcgtaa ccttgttttt tataataagc ctttacacga ttagccattg
    14281 tcccatttgg atggtattgg gcaggaatag acgaaacgcc tgctggttta tttactacca
    14341 gaacgtgttc atcttcatac acgatatcga tcggtgtttc atccaaaagt acggtttcat
    14401 gttctccttc agcaggaatc acaatggtta ccttatcatc cttagctaga gaaaacaaga
    14461 cattttctac ttgatcatta actttgattt gaccgccttg aaattttatt ttcgctaaca
    14521 gaccctttga gatccctttt tcttttaaaa aatattttac ttgctgagaa tgatctttct
    14581 cataatccca tgtaaactgc atatcaatcc tcactaataa aggcatcttt cacacgatgc
    14641 caaaaatgca tatgtcgata agaagcaaaa tggatccgtt cttctgctat tcgataataa
    14701 attgagcgga tatttgcttt tgcgacattc aattgatcaa ccgttacaag ataatcttct
    14761 gtattttcca attttatttc tagccattca tcatgggcaa tcacgatcgg agaacccaaa
    14821 gtacgaaaca ctcgattatt cagtgaagca atctctgcca actgaaaggc attgatacta
    14881 ggatggatca cagcaccgcc gacactttta ttatatgctg tcgaaccagt aggcgtagaa
    14941 atcgtcaatc catctcctct aaaactttca aacagctcgt ttttgatgta tacatcggct
    15001 accatcgtac gattggcccg tttaatcgtt gattcattca gtgctaaaaa atgctggtcc
    15061 ggtgtttcat ccaaataact gatccgaaca tccagcaagg gataactaac actttgttcc
    15121 cggttcgttc gtaagctgtc tactagttct tccagctcgt aatcccgcca atccgtgtaa
    15181 aaccctaaat gacctgtatg cacgcccaag aaacgcacat cattcagaca atgactgtag
    15241 tgatggaacg ccgacaataa tgtgccgtct ccgccgactg agatgaccaa ttctggctca
    15301 tgatcatctc gttgaatccc tgcttgttct aaaagtgcgt tcaaacgttc tgtcacttct
    15361 atagtctttg cttctttatt atgaacgatc gctactttca tcccctgcct cctctctttc
    15421 gctttctttc ttcaaaagcg ttcgatagta gacatcatct gattttcctc ttccatggga
    15481 gaataaccgc tgtgcttctt ggatttcttc acggatactg gacatctctt catccaactg
    15541 ataggcagct tctgccgcac gctgaagtcg ttcactcatt tcttctggaa aaacgccttg
    15601 atatttataa ttcagcgaat gctcaatcgt tgcccagaaa ttcatcgcaa gagtccgaat
    15661 ctggatttcc gccaaaattt tcttctcacc gctgatcagc tgtacaggat actcgatcac
    15721 cagatgataa gaacggtagc cacttgcttt tttatttgtg atgtaatctc gttcttggag
    15781 aactttcatg tcttttcttt tacggatgat ctcaacaact tgatggatat cttctacaaa
    15841 ctgacacatg atccttaacc cggcaatatc agacatttct tcttctaaac gtcccattgg
    15901 aatatgccgt aattgtgatt ttgttacaat actatcgact ggcttgactc tcccagtgac
    15961 aaattcaatc gggacatgtc gattttgttc acggaactgt ttgcggatcc cccgcagttt
    16021 cactttcaat tctgatacag tttgttcata tggtgctaaa aatgcttccc aatctcgttc
    16081 catagctgat acctctactt cattaacatt cattgtttat tctaccatat atgcaaagtt
    16141 ttttcgtact cttgatttat gcattgacca aaaaaagaaa attaggcaaa atagacaaaa
    16201 aggagtcgat caaatgacac aaatgcttga aatcgaatat aaatctatgc tgacaaagca
    16261 agagtacgag atattcatcg ctcactatca attaacagat aaagatttcc gtgtacagac
    16321 taatatttat ttcgataccg ttgatgaaca gctgaagaaa caaaattgtg gcctacggat
    16381 ccgtttcgtt ggtcatcaag ctgaatatac attaaaaaca cctgctgaaa aagggcgtct
    16441 cgaaacaacg gataccttta taacagaaga agcagaagca tttatcttcg aaaaacgtct
    16501 gcctcgttca ggcgcagttt ttcaaaaact ttctgatttg aatatcgatc ctgcttcatt
    16561 aatccaaacc ggaaagctta caacaaaacg agctgaattc cctattgaag aaggactttt
    16621 agctattgat gaaagctggg gagacaatct acacgatttt gaactggaac tagaggtagg
    16681 ggatgcatct gtgggaaaaa ttgcttttat aaatttttta aaacgttttt ctttacctta
    16741 tcgtcctgct aagaacaaga tccaacgtat gcttgaagct aaaaattaga aacctttcat
    16801 tttcacaagg cacttgagtt cattttttta cttttttctg ttaagttata ggatgtgaaa
    16861 gaatctagtc actagttgta tagaatctat tttgggggag attcatatga tcgaaatcta
    16921 tttatttgtt aatcctttag gggaatactg tttcgaaatg gaacaacagt tattacaatt
    16981 tatcgaagaa gagtacggga caccatcaaa agaaaagatg caatttcgct ttcttccttt
    17041 ggtcaatttg cagacaattg gggatgtcat gcaacaaaaa ggaatttctc agaacaatct
    17101 tgaggaacgt aatcacttat tctcaatgac ttattctgca gcattggact gcaaagcggc
    17161 gcagtttcaa ggaaagaaaa aaggccgccg ttttttgatc aaattgcaag aagcagtcgg
    17221 ctgtaatgac ataccatatt ctcaagaact tgctgaatcc atatttgaag aaatcggcgg
    17281 cgatttgaat atgtttaaag aagatcgtca gtctgacttc gttaaagaag tattcttttc
    17341 tgatcaaaaa attgctcggg aaatgggcat cacaaaacac ccttctgcag tcgtttacaa
    17401 ttatgcctgc gaccgtgatt ttggtgtttt agtagaagta aaaggtacaa gtagcctttg
    17461 gagaatcaca gagctgtgta aaacaacaga taactcatat caagttttta atggtgaaca
    17521 tttacataca aatgattctc agttccattc aagaactagc catttacact tgctttctaa
    17581 ttaaaa
//
LOCUS       DACDIH010000054        17534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDIH010000054 DACDIH010000000
VERSION     DACDIH010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.054"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            598..1287
                     /locus_tag="IV281_001943"
     CDS             598..1287
                     /locus_tag="IV281_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8780926.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1274..2482
                     /locus_tag="IV281_001944"
     CDS             1274..2482
                     /locus_tag="IV281_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8780927.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2776..4047
                     /gene="serS"
                     /locus_tag="IV281_001945"
     CDS             2776..4047
                     /gene="serS"
                     /locus_tag="IV281_001945"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP8780928.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="IV281_001946"
     CDS             complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="IV281_001946"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP8780929.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6585..7280)
                     /locus_tag="IV281_001947"
     CDS             complement(6585..7280)
                     /locus_tag="IV281_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP8780930.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="IV281_001948"
     CDS             complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="IV281_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP8780931.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8479..8670)
                     /locus_tag="IV281_001949"
     CDS             complement(8479..8670)
                     /locus_tag="IV281_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP8780932.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8691..9581)
                     /locus_tag="IV281_001950"
     CDS             complement(8691..9581)
                     /locus_tag="IV281_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP8780933.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9568..10335)
                     /locus_tag="IV281_001951"
     CDS             complement(9568..10335)
                     /locus_tag="IV281_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8780934.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="IV281_001952"
     CDS             complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="IV281_001952"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP8780935.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="IV281_001953"
     CDS             complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="IV281_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP8780936.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="IV281_001954"
     CDS             complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="IV281_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP8780937.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14951..15289
                     /locus_tag="IV281_001955"
     CDS             14951..15289
                     /locus_tag="IV281_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780938.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15457..16215)
                     /locus_tag="IV281_001956"
     CDS             complement(15457..16215)
                     /locus_tag="IV281_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8780939.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16233..17051)
                     /locus_tag="IV281_001957"
     CDS             complement(16233..17051)
                     /locus_tag="IV281_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8780940.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="IV281_001958"
     CDS             complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="IV281_001958"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP8780941.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
BASE COUNT         4877 a         3647 c         3206 g         5804 t
ORIGIN      
        1 ttcatcagga gtgtttcccg attcgttttt ggctgccgta gcaactttta tatcatatca
       61 gagagacaag taattgtcaa ctctttttta aaagtttttt cgatttcgtt tttcgaaatc
      121 ttccagcaac tcatttatca taacttatcg tttgttattt gtcaagaact tttttgaagt
      181 ttttttcgat agaatcaagc ttcgcaagtg gttctttttt aacgactcag ttatcttatc
      241 atgtcatttc caacatgtca acaacttatt ttttattatt taataagtta ttggtgttct
      301 tttgaacaaa taataatata tcaacatcac atgtgaaagt caataagaaa atgcttattt
      361 atcaacattt ctagcttaca tgatcattgg ttagttctgg tcctttttcg ttcgtcttta
      421 taactccgag agacgtaaaa tccttttgtt ttcagtttaa tgttcatgtt cttgtcttat
      481 tcacaccaac tagatgtctc accttctaaa attttcctta acaaagtgct gcttttatct
      541 atttctgctt ttttttgcta gaataatagt aacaatatga ctctggagga tttatttatg
      601 aaaattttga ttgctgacga tgataaagaa atcgttgaat tattaagtat ctatgttcac
      661 aatgaggggt atgaagcagt aaaggcttat gatgggaaag aagcattatc taaaatcaga
      721 acaatccctg atattgaatt acttatttta gatatcatga tgcctgagat ggatgggatg
      781 caagtagtga aagagctacg aaaagaatcc caaataccaa tcattatgct gacagcaaaa
      841 actactgata tggataagat caaaggactg gtcgctggtg cagatgatta tgtgaccaag
      901 ccatttaatc cgttagaagt aatggctaga gtaaagtctt tattacggcg tacaaaaatg
      961 cagattgccc cagatcagcc tgatgtatta gaagtaaatg cattaatcat caataaagac
     1021 tctcatgaag ttaaaacaat cgatgggaaa gaaatccagt taacagcttt ggaattcggt
     1081 atcctctact tgcttgctag tcatcctaat cgagtattta gcgcggatga aatttttgag
     1141 cgtgtttgga aacaagaaag tattgtctct gctaaaactg tcatggttca cgttagtcat
     1201 ttaagagata agattgaaga agcaactggt ggtgaaaaag tcattcaaac cgtttgggga
     1261 gtgggctata aaattgacgc aagatagtaa acgagcaact gaaaaacgcc gtcggatcaa
     1321 tttgacttca aaagaaatca gcgaattgtt agcggaagga atcatcacga ttatcctttt
     1381 gcttctatta aatgtttcta ttctggttgt tatcagttcg gtgatcaaca gttcaccttc
     1441 tttgacgaat gcgatttggg attcaaagaa tatttttgca gaacgattga acactgattt
     1501 gttctggaac ggccggaatt ttattattcc ctttttcttt ctattagata ttggtgtttt
     1561 gtattggcgc ttgattcgaa gatatcgcca aatgcaattg cggcatatca tcagcgaact
     1621 tcactacatc gcaaacggaa attatgatca tcgtatccct tttgaactga gtggtgactt
     1681 aagccgagtg gtaacaagta tcaatggatt ggtggatagt accgtagcag cgattgaaga
     1741 cgaacgtaaa atcgaaaaat caaaagatga attgatcacg aatgtcagtc atgatatccg
     1801 taccccactc acgtccatca ttggatacct aggactgata gaagatggcc agtatcactc
     1861 agaagaagat cttttaaagt atacgcatac tgcttatatc aaagcaaagc agatgaaatc
     1921 tttagtggac gacttatttg aatacactaa ggtacgccag cctgctgtcc cggtgaattt
     1981 ttctgccttt gacatgatcc agttgatcga acagttagca gctgattttg agttggaagc
     2041 ttctaaaaaa aatatccaaa tacttgttca atcaaaagta gactccctta tcatggatgg
     2101 cgataccgaa aaacttgtac gtgtcttcaa taatcttttg acgaatgcgt tgaaatacgg
     2161 aaaaggagca acgaagatcg taatcgaagt agaacgtatc ggttcagaag ttgttgcaac
     2221 agtcaaaaat aacggagcaa tgatcccaca acaggcaatc gataatctat tcgaccgttt
     2281 ttatcgtgta gaagaatctc gttcacaagc aactggagga actggacttg gtttagcaat
     2341 cgctcaaagc attgtggctt tgcacggcgg atacatttat gcaaaatcag ataagcaatg
     2401 gacttctttc atcatgcacc ttccaatcaa gaaaaatgaa aaattaccta ttgatacaca
     2461 ggaagttatt gacgaaagct gaaatattcg ctattcttat tacaattatt agaataaatg
     2521 aaaaacacag aacccagtac actttattct ttttcagaga gctgatggca ggtgtaaatc
     2581 agcaaaggat aatttggaag ttccagttcg tttacgtgcc ctttcgaaat aagaaaggtc
     2641 ggttcaacag ccgttatgtg ttcaacaagt gcagctgacg ctgaacttgg gtggtaccgc
     2701 gaatttcaga ctgattcgtc ccaagaataa tgggatggat cagtcttttt ttattttcaa
     2761 aaaggaggaa atcatatgtt agatgtaaaa atgatgcgac aaaatattga agaagtacaa
     2821 gaaaaattga tgactcgggg cgtaaaaaaa gaaacattag ctgaattcat ggaactagat
     2881 gaaaatcgtc gtaagctact agtcaaaagt gaagaattaa aaaaatatcg aaatgatgtt
     2941 tctgctgaga tcgcccaatt gaagcgtgca aaagaagatg cttctgctaa aatcgctgaa
     3001 atgaaagaag tcggtggcaa catcaaagca ttggatactg aaattgctga agtggatgaa
     3061 aaattgaaac aaattgcaac tactttgcca aaccttcccc atgattctgt gccagttgga
     3121 acagatgaag aggacaatgt agaaatccgt cgttggagcg aaccacgtac atttgctttt
     3181 gaaccaaaac cacactggga aattgcagaa aatcttgata ttttagattt tgaacgtggc
     3241 gcaaaagtag ccggtagccg ttttgtctat tacaaaggat taggtgctcg attagaacgt
     3301 gctgtctaca acttcatgct tgatcagcat atttacgaac acggctatac tgaaatgatc
     3361 actccttata tcgtcaacag taaagcgatg tttggtacgg gacaatttcc aaaattcaaa
     3421 gaagatgttt tccaattgga gaatactgat ctaacattaa tcccaacagc tgaagtacca
     3481 ttaactaact attataatga tgaaatttta gatggtgcag atttacctat ttatttcact
     3541 gcactaagcc ctgcttttcg ttctgaagcc ggaagtgctg gtcgggatac acgaggactg
     3601 attcgtcttc accaattcaa taaagtagaa atggtcaaat tttcggatgt agagcattct
     3661 tacgaagaat tggaaaaaat gacagctgat gcagaagata tcttgaaaaa attgcatttg
     3721 ccttatcgtg tgatggcctt atcaactgga gacatgggct tttctgcagc taaaacttat
     3781 gacttagaag tatggatacc tgcacaagat acttatcgtg aaatcagttc ttgttctaac
     3841 tgtgaagatt tccaagcacg ccgcgcaatg atccgttatc gcgataatga tggaaaaatc
     3901 cactatactc atactctgaa tggttcagga cttgcagttg gccgtacagt agctgccatt
     3961 ttggaaaact accaaaatga agatggttct gtcacagttc cagaagcgct cgttccttat
     4021 atggggaatc tgaaagttat taaataaaga ggctgtgaaa aagccgctta gtttcgagta
     4081 ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaattgga cttaataccg
     4141 agaaactggc ttttgaacac catttatcga gaggttgtga aatcagcgaa ttaacctgag
     4201 tattagagaa aaaaatggaa aaatagtccc tcatatttta ccgtttgttg aactaatacc
     4261 gagggtcagc tgattgaaca ccgtttagtg gagaggtcgt aagcctaccc cccagctcct
     4321 agttacaagg acaaattcaa aaaacagctc ttcatatttt atgaatgttg tacttgtgat
     4381 tcggaggtgg caagggaaca ctgtttattc aaaaagaggc tgggacagaa gcgtttaact
     4441 ccaagaaata agaagaaatt cacgaaaatt gcttttcaaa tttttgtgaa tttcttctta
     4501 tttccgaagg agttgcttct acttccgccg tttatacgtg tttagagcgt gagacaaaag
     4561 tgttctttac ttttgtccca cgctctttaa tgtttttaag attcaactga atagtttggt
     4621 gcttcttttg tgatttgtac atcatgaggg tgtgattctt ttaatccact gccgctcatt
     4681 tggataaatt gcgcttcatc acgtaattgc tgtagattgg ctgccccaac ataacccatt
     4741 cctgaacgca aaccgccgat catttggaaa ataatatctg caacacttcc tttataagca
     4801 acccgacctt cgattccttc tggtactaat ttatttgctt cattgacact accttggaag
     4861 taacggtctt ttgaaccttt ttccatcgcg cctaatgagc ccatacctcg gtatgttttg
     4921 aatcgacgtc cttggaagat ttcgaattca cctggtgatt catctgtccc tgccaacata
     4981 cttcctagca tcactgcatg tccgccggca gctaatgctt tgacgatgtc accagaatat
     5041 ttgatccctc cgtctgcaat aatcgctttg ccatattgac gagcaactga tgctgcatca
     5101 taaatagctg tgagctgagg tacacctaca ccggctacca cacgtgtcgt acaaatcgat
     5161 cctggtccga taccaacttt tacgacatct acacctgcat catataaagc ttttgtcgct
     5221 tcagccgtcg caacgtttcc tgcaatcaat gttgcatctg caaaagttga acggatttct
     5281 tgaatttttc ggatcacacc tgcactatgt ccatgagctg tatcgatgat gattgcatct
     5341 gcccctgctt ctaataaagc attcgcacgc tcaaatgtat cgcttgttac accaacggct
     5401 gctgctacaa gcaatcgacc atgttcatct tttgctgcat ttggaaattc gatcactttc
     5461 tcgatgtctt tgatcgtgat caatccgctt aaacgacctt cgttatctac gattggtaat
     5521 ttttcgattt tatgtttttg caaaatcttt tctgcatctt ttagagaggt acctactggg
     5581 gcagtcacta agttgtcttt tgtcatcaca tcagcaatcg gcatgtgata atctgttaca
     5641 aaacgcatgt cgcggtttgt aattatccct accaattttc tgttttcaag tgtttcaacg
     5701 attggcacac cactgatacg atatttgctc atcaattctt ctgcgtctgc tacaagatgc
     5761 tgtggcgtta agaagaacgg atcaatgatt acgccacttt cagaacgttt gacttttctt
     5821 acttcatccg cttgctggct gatcgtcatg tttttatgga tcacacctag tccgccttgt
     5881 cttgccatag caatggccat cttgctgtcg gtaacagtat ccatacttgc actgatgatt
     5941 ggaatattca acttaatatt tttagctaat tgtacgctca tatccacttc gtttggtaac
     6001 acgtgacttt ctgctggtat caataagaca tcatcaaaag taaatccttt tttcgtgaac
     6061 tttgtttccc agttggacat ttttagtacc actcctctaa tttagtttat ctattaaaat
     6121 aattgatttt ttcatttaag tcaatgatct acttggaatc aagttagctt tctcatagaa
     6181 taccaatata aagcgtttac atctgtgtgt attaaaagag attgtaaaaa agctgtcctg
     6241 catcaagtaa taagaacagc caatcaaaaa cagcttctca tgtttttgaa ttggttgggt
     6301 ttattaccgc agatgcaaac ttttgaacac cgtttagtgg agaggctgtg aaaaagctgc
     6361 ttagtttcga gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt
     6421 ggacttaata ccgagaaact ggcttttgaa caccgtttat cgtaaaaagg gactgtgaca
     6481 agacttttgt cacagtccct tactattatt taactatgga taaacaaaat gaggttcttt
     6541 actaccccta gatatctagc aaaatagctg atctttttgc tagtttatct tcttgggacg
     6601 gataaacttc caataatccc gtattttttc ttcaagtagt aacggattgc taaagcagtt
     6661 gccccgataa tgataactac tactggatct aatactggat tgataactgg tggcaatagt
     6721 gttgtcgcag aaaacagtac gacccaaaga agtgtcgttc cacctaggat aagcaatgtc
     6781 ttacccattc ctggtttctt gcttcgatct gctccgggat attcatattg atagatgtat
     6841 ttgtacatca tataaaagac ccagccaccg attgcagaac ctagaacgag tgtagaaaag
     6901 ccataagctg gcgttgttcc acgtgaaaac aatccggtaa tcgcaatcat gatacttaat
     6961 agaccaagca acagcaacgt attatctaac cacatcaata aaggggtgga tgaatttttt
     7021 acttctgctg gcttattgat gattgcttcc gttctttcag agactgtccc aaacaattga
     7081 cgggctgttc ttccgccttt ttgttctgca actaaagtag gcagaatgtc atgaagggct
     7141 tttacttttt cttcttcaga caaattcgct gcttctaaag attttttcaa atcaaaaatg
     7201 tactgttgat ttcttttggt taattttgtt tctaaactat tgttttcttt tacgattgct
     7261 cgtaattgtt ctggttccat tatctaatag tcctccttag ccgttccttt tctcaacggt
     7321 cgtcacaaaa gacattctac catattctac agctagtttg atagcccttt tacacattaa
     7381 agcggaataa cattacgtcg ccgtcttgta cgacataatc ttttccttct aatcgaacac
     7441 gtccagcttc ttttgctgca tgcatgtttc cgtaatgatc caagtcttca aaagagacag
     7501 tttctgcacg gataaatccg cgttcgaaat ctgaatggat gattcctgct gcttgtggtg
     7561 ctttgattcc ttttttgaat gtccaagcac gtacttcttg ttccccagca gtaaagtaag
     7621 tagctaagcc taacaaatca taagcagcac ggatcaactg atccaaacct gattcttcga
     7681 ttcctaaagc ttctaaaaat tccgctttgt cttcttcatc taattctgcg atttcttctt
     7741 ctgcacgagc actgaccacg atcacttctg cattttcttc tgcagcaaag ttgcgcactt
     7801 cttgtacata atgattcgcg tctgcatctg ctacgtcatc ttctgagaca ttggctacat
     7861 aaagaattgg ttttgtcgtc aataaaaata gtgatttaac gattttttgt tcttcttcag
     7921 taaattcgat cgttcgggca gattttcctt cttccagtac aggtttgatt ttatccagta
     7981 cagctagttc tgccactgca tctttatctt tggttttagc aacttttgct acacgagtat
     8041 agcgtttgtt gatagattct aagtctgcta acactagttc caagttgatc gtatcaatat
     8101 cttctagtgg atcaacacgt ccttctacgt gagtaatatt atcatcatca aaacagcgaa
     8161 caacgtgaca aatagcgtct acttggcgga tatggcttaa gaattgattt cccaaacctt
     8221 ctcctttact tgcgcctttt acgattccag caatatctgt aaattcaaaa gtcgtcggaa
     8281 cagttttttt cggatgtaca agttctgtta agcgttgcaa acgccagtca ggtacttcta
     8341 ccatccctac gttggggtcg atcgtcgcaa agggatagtt tgctgcttct gctcctgctt
     8401 ttgtgatcgc attaaacaaa gttgattttc ctacgttggg caacccaaca attccagcag
     8461 tcaatgccat aaatttattc actctttctc tgcatttttt tcaattactt tttttaattt
     8521 cttttcaaaa tctcgacggg tcatcatgac gatatgtccg caattcatac atctgatttt
     8581 gatatctgcg cccattcgaa tgacttccca gcgattcgtc tggcaagcgt gaggtttttt
     8641 catttcaacg atatcgtgca agtcgtacat aaaaatagcc tcctttttgt ttattcttca
     8701 tcaaattgaa tattcaagat atcaagaatt cgagtcaaat ctttttgaga aagatattca
     8761 atctcgatct tacctttccc atttttttct tggatagcta cacttgtacc aaatttatcc
     8821 atcaaacgat cttcgctttc tcggatataa tatggttttt cctttgctag ccttgggatt
     8881 tttttctttt cttttccatc attttgattt aactcagaaa caagctgttc cagttggcgg
     8941 acagttaaat tttcagttac tgcacgattg gctaatttca agatcaatgt tttatttttt
     9001 aaacctaaca gcgtacgggc ttgtcccata gataaacgct gatcttgtac catttccttg
     9061 acaagatccg gtaaagaaag caagcgcaaa taattggcga tataaggtct gctctttcct
     9121 aaacgactag ctacttctgc ttgtgttagt ttcaggtttt tcatcagcat ctcatatgct
     9181 tctgcttctt ctaacggatt cagatcttct cgctgcaagt tttccagcac tgctacttgc
     9241 atcatcgctt cttcgtcaaa gtctctaacg atagcaggga tcgtttcttt ttcagctagt
     9301 ttggacgctc gaaaacgtcg ctcccctgca ataatctcat atccttttac tgcggaacgg
     9361 cgtacgatga tgggttgaaa gacacctgat tgttcaatag aattagctag ttcctgcaaa
     9421 gatacttctt caaatgtttt acggggctga tacggatttg gtcgcagctc attcaatggt
     9481 agctggacaa cggtatcatc ttttacatcc accgtctcta agttttctaa atcttgaaac
     9541 agtgcatcga ttcctcggcc taatccttta cttttcttca cgacttacca cttcctttgc
     9601 caatgcttgg tagacttcag ctcctcgaga acgtggatcg tagtcgatga tcggttttcc
     9661 atgactaggt gcttctgata aacgaatatt tcttggaatg atcgtttcgt aaactttttc
     9721 gcggaagtat ttacgtactt cttctacgac ttcatttccc agatttgttc gtgcatcata
     9781 cattgtcagt aagactccct cgatctctaa ttctggatta aagtgttttt ggactaaacg
     9841 aaccgtattc agtaattggc ttaatccttc caatgcgtaa tactcacact gaacaggtat
     9901 caaaatcgaa tcacttgctg taaatgaatt gattgtcaga tggccaagag aaggcgggca
     9961 atcgatcaaa atataatcat actgactgct tacttctgct aaagatcctt ttaatcttga
    10021 ttctcttgcc atcattgacg tcaattcgat ctctgctcct gctaattgga gagtcgcagg
    10081 cacaatactc aaattttcgt gttcagtgat caaagttgcc tcatcaattg gcaattcatt
    10141 gactagaaca tcatagatgt ctcttgtcac atcaggtttt ctgatcccga caccactggt
    10201 agcatttcct tgtgcatcca tatccactag caatactttt ttgccaaggc tagcaagaca
    10261 agcacctaga ttcaccgtcg tcgttgtttt gccgacgcca cctttttggt tcgcaacaga
    10321 tattatttgt gccatctctt ctcctcctat ttgattggct gcttattagg cagtccgggc
    10381 tttcttgggt atttctttgg ggttggtttc tttttttgaa taacgatgat atgccgttga
    10441 tcagagacat tcggcaaggt aaccgcctct tctttgagga atttaccacc taataaagca
    10501 atcgcttgtt ttgcttctgt caattcctct tcactttttg ctgctttcaa tgcataaaaa
    10561 tagccttctt gtttagccaa gggcaaacac aattccgcta acacattcaa acgagcaacc
    10621 gcacgagcag tgacaaggtc aaatgatgca cgaaagtgag gattttgccc aaatgtttct
    10681 gcccggtcat gatataagga agcatcgatt cctaataatc gggttaactc agataagaag
    10741 gttattcgtt tattcagaga atcaacgatc gttatctgca gttctggaaa ggcaattttt
    10801 aaaggtatgc taggaaaccc tgcccccgaa ccaacatcgc ataaagataa aggttgcgta
    10861 aagtctactt caaatgctaa tgtaagtgaa tcgtaaaaat gcttcagata tacgtctttt
    10921 tcatccgtaa tagctgtaag gttcatcttt tcgttccatt cgactaatgt ccgataataa
    10981 atagcgaatt gattcatttg atgatcggta agctctatcc ctttttctgc caaggattgg
    11041 cgaaattctt ctggtatcat aaatattcct ttcattgtga aacagcaatt gtttcacaga
    11101 ttctctccct actttaacgc aaaatctcta gacatgcaat ccttccatat ggattcttaa
    11161 aaatttgaaa agtaagaaaa gccgatagac tttttctttc tagtatgact cataaaatat
    11221 acacagaaga gagaactagc tttgagcaat ttctgcaaaa actagttctc tcttctattt
    11281 attattacgt attacgctgg ttcactgact ttcgcaatct tcccttgttc gatatagacc
    11341 attaggatac ttaggtctgc agggtttacg ccactgattc ggctagcttg ggcaattgtt
    11401 tctggttgga ttttctgcaa tttttgtttt gcttctgttg ctaatccgtt gattgctgcg
    11461 tagtcgatgt tttctggaat ctttttcgct tccatgcgtt ttaatttttc tactttttcc
    11521 atggcttttt tgatgtagcc ttcatatttg atttggattt ctacttgttc gatttctttt
    11581 cgtgtcaatt cttcattttc tggaataaac cgtagaagtt ctgcataagt aatctcagga
    11641 cgtcttaaaa agtcgcttgc tagtacacca tcttttaatg gtgccgcatt aatcgttgct
    11701 aaaaagtctt ggacttcttc tgttggtttg attcgtacat tcatcaaacg tttgatttct
    11761 tgttcaactg cttctttttt cttcaaataa ttcttgtatt gatcttcttt tactaagcct
    11821 acacggtgac ccatttctgt taatcgcaaa tctgcattat catgacgtaa aagcagacgg
    11881 tattcagcac gtgaagtcag caaacgataa ggttcgttcg ttccttttgt taccaaatca
    11941 tcgatcatga caccgatgta accgtcactt cgtttgatca ctaaaggttc ttttccctga
    12001 actttcaaag ctgcattgat accggcaatc agcccttgtc cagccgcttc ttcataaccg
    12061 cttgtaccat ttgtttgtcc agctgtataa agattttcaa cgactttagt ttctaatgtc
    12121 ggacggagct ggtgtggctc tactacgtca tattcgatag catatcctgt acgcatcatt
    12181 tctacttttt ctaatccagc aatagaatga agcatgttga cttgaacatc ctctggtaaa
    12241 gaagtagata aaccttgaac atagacttct tccgtattta agccttctgg ttccaaaaat
    12301 aattgatgac gtggtttgtc tgcaaaacgc acgatcttat cttcgatgga tggacaatag
    12361 cgagcgccta ccccttcaac gatccctgta aacattgggg cacgatgtaa gttgtctcga
    12421 ataatttggt gagtcgtctc acctgtatag gtcagccagc aagattcctg atctagttta
    12481 taggcactat ctggtgtgct gaagctaaaa tgattcggct ctttgtcacc aggctgctct
    12541 tctgtaacag aatagtcgat cgtacttgat tttactcgag gcggtgttcc tgttttgaac
    12601 cgttcgatag caaagccaag ttcttttaga tgattcgcta gtccaataga aggttgagag
    12661 ttattcggac ctgatgaata tttcaattca ccgataatga tttctccgcg aagtgctgtt
    12721 ccggctgtaa taacaactgc tttggcccgg tagctcgctc ctgtggacgt cactactcca
    12781 tgacaaacac catcttcgac gatcagttct tcgacgattc cttgtcttag cgtcaaattt
    12841 ggttcttttt cgatcgtatg tttcatttct gttgaataag catgtttgtc tgcttgtgct
    12901 cgcaatgctc ggacagctgg gccttttcct gtgttcaaca tacgcatttg gatataggtt
    12961 ttatcgatat tcttacccat ttctccgcct aacgcatcga tttctcgaac gacaacgcct
    13021 ttagctggtc cgcctacaga aggattacat ggcatgaatg ctaccatgtc caaatttatt
    13081 gtcagtaata atgtttttga tcccatacgt gcagcagcaa gtgcagcttc tgaaccggcg
    13141 tgtcctgcgc caacaacgat cacgtcatac gcatctgctt cataatgttc caatcgtttg
    13201 ttcctccttt tattttccta aacaaaattg actgaataat tgagtgatta gctcatcttg
    13261 tacactgtcg ccgacaactt ctcctagata gtcccagcaa cgcgtcatat ctatttgaac
    13321 gagatccaca ggcattcctg aatcaatacc tgcaatgact tcttcaagtg agagggaagc
    13381 tttttctaac aacgcgatat ggcgggtatt agaaacataa gtagcgtctc tttctcctgt
    13441 ttctccgctg aagaataaat cagaaatagc tgattccaaa cgatctagtc cgtcgttttt
    13501 agctacggaa attgaaaaaa ctggttcatt ttcgatcaat ttcttcaatt tttcttgttc
    13561 cagctgtgct ggcaaatctg ttttatttag aagaatgatc cgtttcaatc cacttgtcgc
    13621 ttccaataat tgttcatctt ctgctgtcag tggttcgctt tggtttagaa caagtaagat
    13681 caagtctgct tctgctaatg cttttcggct tctttcgacc ccgatccgtt ccaccacatc
    13741 ttctgtttcc cgaattccag ctgtatcgat tagcttgagc ggtacccccc gaacattgac
    13801 atactcttcg attacatctc gagtcgttcc tgcaatatcc gtaacgattg ctttttcttc
    13861 tcttaacaaa tggtttaaca aacttgattt gccgacattt ggccggccga tgattgccgt
    13921 acttaaccct tcccgcagaa ccttcccttg cttggatgta gcaagtagcg cttggattct
    13981 ttgttggatc atctgcgctt tttcaagcaa taacttcgta gtcaattctt cgacatcatc
    14041 atattctgga taatcaatat ttacttctac ttgtgctaat gtctctaaaa tctcttgtct
    14101 caatgagcgg atcaaagcag acagattgcc gtctaactga ttaagagcta gtcccattgc
    14161 tttgtctgtt tttgcccgaa tcaaatccat cacagcttct gcctgtgaaa ggtcaacacg
    14221 accattcaag aatgctcgtt ttgtgaattc gcctggctca gctaatcttg caccttctct
    14281 taacaatagc tgcaaaattt gattgactac aacgatccca ccgtgacaat tgatttctac
    14341 tacatcttcc cgcgtaaatg tctttggtgc tctcatcacg gagaccatga cttcatcgac
    14401 aagctgattg ctcttgggat caacgatatg tccataatgg atcgtatgac ttggtacttc
    14461 tgataatcgt ttgtttccag attgataaac tttatctgct agttgcacag cttgatcgcc
    14521 acttaaacga acgatactga ttgcaccttc tccaggtggg gtagaaattg ccgcaatcgt
    14581 atcaaattct aaagtaattt cagccatttt ttcacttcct ttttcatttt gcgcacaaaa
    14641 aaagtgccca ctccaccctt tatgcacaat aaacggtgaa cttcgcactt tgactgcttg
    14701 ttcttgatta aattctggtc atagattatc acacttgcta taaggaatca agtgtttaaa
    14761 acaattagat gaagttgagc ctgttctgct tccattacct tatatttaat tgtccttgga
    14821 tgtctctttg cgttctttgg tttattcgaa gagaagatac tgtataaaca aggctttatc
    14881 tttcttaaaa aaaacggtac actaaatggt agcgctatct aaactataca cttttgaaag
    14941 gagaaaatat gtgagcccgt tcagatctgc tgcattggaa gaagaaagtg accctcggat
    15001 caacgatgtc gttttatcta cagggaatat caaccagaaa tatatcgtcc gcgatatcgt
    15061 ttttgttgtg gaatgcttag aagctgattt gtttgaccca tcttttccca aagatgacct
    15121 tttcttaaca acgaagagga aattaaagac gaaggtactt gaatatggtg cgaatgcagt
    15181 aatcaactgt cattttgagt atcagcaaaa aaaacgcgac ggaaagtatt ttctggaact
    15241 ttttgcttat ggaacagtga tccaatttat ccaaacaacg attggttaaa tgaaacagac
    15301 aaaaaaagcg gatcaaaact gaagacagtt tcgatccgct tttttctatc aaagttagtg
    15361 actacttcat cgtatactct gataagaatg atttctagta aaatatactt ggtttgaaaa
    15421 actttgtcaa cgtgatttat tcaaaaagta atggaatcaa taatattttt tagcaggttc
    15481 tactactaaa taacgataag gttcctcacc ttccgaatgt gtttttacat gttcatcttt
    15541 gcttaatgca gcatggattt gtttacgttc aaatgcaggc attggttcta agaaaacagg
    15601 ctgccctgtc cgttttactt tttcagccgt gcgatcagct aaacgctgaa tgatctcttg
    15661 gcgcttttca cggtaattgc ctacattgac tactacagac agcttatttc ttgctaaacg
    15721 atggatgaag acttgtgcca gatattgcaa tgcattcagt gtttttccat gtttcccaat
    15781 cagtatgcct tgcttttgtg tttctaaatg gaaaacaacc atattatttg attctctttc
    15841 cattttgaca agagctggag catcaagctg ttttgaaatt tcagtaagat aaactgccaa
    15901 ttccgttaaa gctgcttcat cacctagctc acttacttct tctgtttttt cttcaagaat
    15961 cgtttcgttg actacctcga tctcagctgt tggttcttct tccaaaattt cttctacagt
    16021 ctgttctact gtttcagaaa ctttttcgct gatacttggc tggatcgaaa gccgtgcatt
    16081 ctttcgaccc attcctagga aacctttttt tccttcttcc agtatttcga tttcgacttg
    16141 gtctttggcc aaacccaaat cagccagacc tagttggaca gcttcgtcta ttgttgcagc
    16201 ttcataaatc ggcattagca ttacctcccc tattatttcc gttttttctt aggattcatt
    16261 gctttcttaa gagcacgttc tctttcgcgg attctccgtg cttcttcttc acgctctttt
    16321 cgaattttga aagggttgtt gatcagcagc gtttgtacaa cttggaaggc attggaaact
    16381 acccagtata atgaaagacc gctggctaaa ttgacaccca tcactaaaat cattacaggc
    16441 atcacgaaat tcatgatctt cattgatgca ttcgattcaa tttgactcat gctagataaa
    16501 tacgtacttg caaaagtaaa gattgctgct aaaatcggca aaataaatgt tggatccgcg
    16561 ttccctaagt tcaaccataa gaaatgccct tgtttcaact ctggtacacg tgaaatagat
    16621 tgccataacg ccatcaagat tggcatttgt actaataaag gaagacaacc agcatatgga
    16681 ttgacgccat tttctgcata taaacgctgc tgctcttctc ttaacttact ttgtgtttcc
    16741 ggatctcttg aactatattg ttgctgcagt gcttttaatt tcggttgtaa ttcttgcgtt
    16801 ttacgcatgc tctttgtttg gaaatgcatc aacggcaata ggatcacgcg gatgatcagt
    16861 gtaaataaaa tgatccctat ccctgcatta ccaaatgaca acgccttgat cgcctcagca
    16921 aagtaataaa caatataacg atcccaaatt cctgtactct gtgcgctgac ttccccagtt
    16981 ccgcaaccgg ataagacgat gaccaaacta acgataccgg ccatcaaaag taaacgtttg
    17041 tatttcttca cttactcttt tcctctctgt cgattatttt tgcaagtttt aagacgtgga
    17101 ctaaattgga gcgaacctca tcataagtca accccttcac acttggtcta gcaatcacaa
    17161 taaaatccaa ctccggatca atttcttctt ttatagaata aactgctgct cggatttttc
    17221 gtttgacttc attgcggttt accgcgttgc cgaccttttt tcctacagat aaaccaactc
    17281 gaaaatgagg ctgtcccggt ttttccaaac ggtaaacgac gaatttgcgg ttagcaaaag
    17341 aatctcccgt atggaacacg gcttgaaact ctcgttcttt ttttactcga tacgcttttc
    17401 tcattagttt tctttccttt tctatctctc acttacgctc ataatcatac cataattacg
    17461 attgtccttt catcatttcc ctaaaaaaga gtataaaaaa aaccactgaa accccagtgg
    17521 cctaagctga aaga
//
LOCUS       DACDIH010000055        17133 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDIH010000055 DACDIH010000000
VERSION     DACDIH010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.055"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..127)
                     /locus_tag="IV281_001959"
     CDS             complement(<1..127)
                     /locus_tag="IV281_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAP8780942.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(231..542)
                     /locus_tag="IV281_001960"
     CDS             complement(231..542)
                     /locus_tag="IV281_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8780943.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(544..741)
                     /locus_tag="IV281_001961"
     CDS             complement(544..741)
                     /locus_tag="IV281_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP8780944.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1135..2862)
                     /locus_tag="IV281_001962"
     CDS             complement(1135..2862)
                     /locus_tag="IV281_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8780945.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2855..4048)
                     /locus_tag="IV281_001963"
     CDS             complement(2855..4048)
                     /locus_tag="IV281_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8780946.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4235..4879)
                     /locus_tag="IV281_001964"
     CDS             complement(4235..4879)
                     /locus_tag="IV281_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8780947.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4973..5107)
                     /locus_tag="IV281_001965"
     CDS             complement(4973..5107)
                     /locus_tag="IV281_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP8780948.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5109..7241)
                     /gene="feoB"
                     /locus_tag="IV281_001966"
     CDS             complement(5109..7241)
                     /gene="feoB"
                     /locus_tag="IV281_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP8780949.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7238..7711)
                     /locus_tag="IV281_001967"
     CDS             complement(7238..7711)
                     /locus_tag="IV281_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP8780950.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8112..8336
                     /gene="nrdH"
                     /locus_tag="IV281_001968"
     CDS             8112..8336
                     /gene="nrdH"
                     /locus_tag="IV281_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP8780951.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8495..10654
                     /gene="nrdE"
                     /locus_tag="IV281_001969"
     CDS             8495..10654
                     /gene="nrdE"
                     /locus_tag="IV281_001969"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP8780952.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10704..11669
                     /gene="nrdF"
                     /locus_tag="IV281_001970"
     CDS             10704..11669
                     /gene="nrdF"
                     /locus_tag="IV281_001970"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP8780953.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11758..12897)
                     /locus_tag="IV281_001971"
     CDS             complement(11758..12897)
                     /locus_tag="IV281_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8780954.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13206..13919)
                     /locus_tag="IV281_001972"
     CDS             complement(13206..13919)
                     /locus_tag="IV281_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP8780955.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14173..14874)
                     /gene="nagB"
                     /locus_tag="IV281_001973"
     CDS             complement(14173..14874)
                     /gene="nagB"
                     /locus_tag="IV281_001973"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP8780956.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15108..16640
                     /locus_tag="IV281_001974"
     CDS             15108..16640
                     /locus_tag="IV281_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8780957.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16831..17010)
                     /locus_tag="IV281_001975"
                     /pseudo
     CDS             complement(16831..17010)
                     /locus_tag="IV281_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(17030..>17133)
                     /locus_tag="IV281_001976"
     CDS             complement(17030..>17133)
                     /locus_tag="IV281_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP8780958.1"
                     /translation="LESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         5317 a         3523 c         2929 g         5364 t
ORIGIN      
        1 atgaaaaaac acattccact aaatttgatg ccggttttcc ttgaggtttt gttccagcac
       61 ctcctttaat ttttttaagc tcttgatctg aacattgttt agcatttagt ctattttttt
      121 cttccattac gattccccct acaaactata aatactttta tgtcactgat tcaatatacc
      181 tatttttcta atcaaaaaat tttgagacat atttaatatt ataagtatca ttaaaattga
      241 gatttatctc cataatctgc tcgatataaa ccatttattc cagcagttct tccaatttca
      301 ctcaaatttc ttattaattc ctcttgatat tcagtgaatc ttattttatt agtataagca
      361 gtaaaatata gataattcac caatctattt attaaaggac tgggatctaa ttcatatttt
      421 tgtttttcta attttaaata taccttttgt aaaacctcaa gaatatcaga atatttagga
      481 tctttactta tagatatatc attatataat tcatgaacaa tttgcttagc attttttttc
      541 attttagcac ttccctggaa ttgctccacc taaaaaacca cctatagaca ttcctgcaat
      601 acaagtagtt gccttggccc aatcgaccgt acatttattt ttagtgcaat acactccatt
      661 tccataatat tttccactat gagtggtacc cccatagata agttgtgtct ctttaataga
      721 caaaattttt aaatgtttca taatctaatc acccctaaat taatgttttt agactattat
      781 tctaacattc attaaatttc aaaaaacaat tccttcttga aacgtagcaa aataattcct
      841 gaataagaat ttaatcactt ttaagtatat aaactatgta tattctttaa tattttttga
      901 gaatgcactt cgtacataat aaactttaat cacattagtc tttcatacct acgatcgatc
      961 tctttgactc gcacccataa attcataata gtttgtcggc gtctttgaac gtttcgaaga
     1021 tgagctacgc attctctttt tttatagaca aaaaagatag agacgaacat ggagcttcca
     1081 aatttggata tttgatccaa ttttggaagc tccatttatt tcgtgtctgt ctttttattt
     1141 tttccagctt tcgactaatt cttggttctc tttgatccag tcttttgctg cttgctgtgg
     1201 atcttttcca ttgttgattt ccaacataac tttttccata tctttttctg accaatggaa
     1261 attgtctaat actttgtaag cttctggctg gtcttctttc aatccttttc gaaccatcgt
     1321 atggatctgc tcttcgcttc ccattccgtt ttcaggatct tctaagtatt tcagatcata
     1381 tttggcaaac atccaatgag gtgtccatcc tgtaactaca attggttcgt gttttttgat
     1441 cgcttgacct agtgcaacgg tcattgctcc tgaagaagat gtctctactt tccagtcttt
     1501 caagttgtca tatttttgga tcgtgttttc agcagccgta actacaccag ctcctggctc
     1561 gattcctatg attttcttac ctgcctgatc ggttaactca tcgatcgaat ttacatccat
     1621 gtaggctggc actgctaaac caacttttgc tcctgtcaaa ttggcaccta ggtcttcgac
     1681 ttgatcttta tattgtgcat attgtgaacc atgagtttta ggtagccaag ctgaaaccat
     1741 ggcatctgac tcgccatttg aaacagattt ccacatgatt gaattatcta atggcgtcat
     1801 cgttacatca tagcccattt gtttcaatac ttcaccgact acatttgttg aagcaacttc
     1861 tgtatcccat tcaacataag ataagttgat acgttttgtt tctttggcag atgaaaaact
     1921 ccctatacca atcgcaccta agatcactac tactacagca ccaatgatca gtccttgacg
     1981 ttttttactt ttctgtgttg ttgctcctgc agctttttta ttgtttggct ggttcaaatg
     2041 ctgtgtaaaa cggtcaatga tgattgctaa gataactaat gcaaccccgt tgacaaatcc
     2101 attcccgact tgtgctcttt gcaatgctga taatacgccg cgtcctaagc cgggtgcacc
     2161 gatcatagag gcaataacga ccattgaaag tgacagcatt gttgtttgat tgatccctgc
     2221 catgatcgta cttttagcta aaggcaattc tagtttgaat agtttttgac gacttgtgct
     2281 cccgaatgaa tcagaagctt ctactaattc tttaggaact tgtcgaatcc ctaaattcgt
     2341 aaaacgaacg gttggtggca gagcaaagat gactgaggca aatacccctg gaaccatccc
     2401 gataccgaaa aatgcaactg ctggaatcaa ataaacgaat cctggcattg tctgcataaa
     2461 gtcaaggatc ggtttgataa tactttgggc tttactgctt tttgccatca agatccctaa
     2521 tggtacccca atgatgatcg agatcacact agataaaaga acaagtgtga cagtattcat
     2581 caaatcattc catagacctt ggttataaat aaaccataga ccgattaatg tgaacaacgt
     2641 aagtcctgga cgtttcttag aaataaagaa agctgctacc gtcaataaca agataaacaa
     2701 aggagctgga atcactaaca gtaaatttgt aatccccgac atcactgcct gtccaatcgt
     2761 ttgtaaaaat gaaaataggc cagaaaaagt tgtcgtaaac cattctgtga tattttctac
     2821 ccagtttgcc actggtaatt gatagttcaa tagattattc atgttcactc acctcgcttg
     2881 tttctgctaa tgcttcaatc acactacctc ggatgacgac accttttaat tttccatcct
     2941 ctacaactgc aagtggagtc ggtgagtcat aaataatatt gaaaatatca cttaccacca
     3001 tgtctttccc aatcaccgtc acattttcat ctaggaagtc gatcaatgga cgtttttgct
     3061 tcctagcttc taacgcttga tctgcagtga tgatcccttt caggtgtctc tttctgtcaa
     3121 cagccatcag catgctgact tcttcattac gcatccttgt caatgcaacg tttggtccat
     3181 cagattcgat attcgtcgtc aatgcaggta ccatgatgtt ttgagccgta agcactttgg
     3241 aacgatcgac ttcttccaca aattcacgta cataatcatt tgctggattg gtcaatattt
     3301 cttcacctgt tccgatctgc atgatttcgc cgtctttcat caaggcgatt ctgtccccaa
     3361 tccgtaaagc ttcattcaaa tcatgggtaa tgaaaataat cgttttttga acattggctt
     3421 gcaggtccag taattcgtct tgcatttctc gccggatcag cggatcaaga gccgagaacg
     3481 cttcgtccat cagcaagatc tcaggatcat ttgctaaagc tctggctagg ccaacccgtt
     3541 gttgcatccc accagataac tgactaggat attggtcttt aaatgaaagc aaactagaat
     3601 tttctaatgc tttttctgct ttttcttgtc gttcttcttt aggtacacca cggacttcta
     3661 acccgtattc tgtattttcc aagatcgttc gatgtggaaa cagaccgaag ttttggaaaa
     3721 ccatattgat tttatgacga cggacttctc gcaattcttc tttgcttaac ttcgatacat
     3781 cttctccatc aatatagata ctgcccgaag ttggctcaat cagacgattc aatagacgga
     3841 tcaatgtgga tttcccactc ccggaaagcc ccatgatcac gaagatttct ccttctttta
     3901 cgtcaaaatt cacgtcatac acaccaaccg ttgcaccggt tttctttaaa atctccgttt
     3961 tcggctggtg atcattcatc atatctaaag cggcttgtgt ctttttacca aatatctttg
     4021 ttaaatgttt tacttctact tttaccaaac aaattctcct tttttatgta aattcatttt
     4081 tctttctgaa aaaagtgacc acactatgtt aacatcatgt agtcactttg tcaaaaatga
     4141 aagaaatttg cttgtccaaa ctgaaaaaaa cttggctaaa tctttgtaaa taaaaggttt
     4201 agccaagtta atattttttt aatatttcac acaatcattc tgcaagctga tgctccgggt
     4261 aaaaaaagtt tgtcatacgc tgtagtgtat attcatttcc aataaaatga acgatgtccc
     4321 ctgccgtaaa tttagcatag gggccaggtg aaacaagcag ttcttcatta tgagaaattg
     4381 cgataactgt ggcgccagtc gcctgccaga tattcaactc gctaattgaa cggtccagat
     4441 gcattgcttt ttctgataaa gataattcaa acggaagtaa aggattcatc ttatgtattc
     4501 gcttcgtttg attgaccagc tgatccaata attgagaaaa atgattcagc tcatcttgtt
     4561 gcttctccac acttgccatg atttctgctt ttgtatcttg gatggattgt acatcttgat
     4621 attgcaatag gaaatctttt gctttttcct tcgaagcaac aaatgcccca cttccatgcc
     4681 gtacttccat gattccaaga tctaccagca cattgatagc cttgcgggta gtctcaggag
     4741 aagcattgta cgtactggca agtgttgagc gggcatggat cttctcaccc actttatatc
     4801 ggttttcaac gatacgttcg gctaaatcaa cagcaatctg ctggtagcgc gggcggacga
     4861 tttttttctt tccagacatt ttctttcctc tttctttttt tactttccct catcttacta
     4921 tgctctagca aaataagcaa aaaaggggag aatagcctct tgcaacgact gatcatcgct
     4981 tgatctgttc cttttttact gggcaagctg tttgacagtc atcacaattc ttaccggatt
     5041 ttaaacgagt atagacaatt gcgcctgcac ttccaaagat taacacactt aataagaatg
     5101 tcgccatcct attcctctcc ttctaatgac aatatagtat aaaactggtt cttggatttt
     5161 ggctgacgga acaaaacata aacgagtcct atcatcagta aaatagcaag atacgtccct
     5221 aacgctacct gtccaccctc caataagtgt cccaactgat aaatcacaaa actaactaaa
     5281 taagctaaac cgcattgata gcctatggcg atccaagtcc atttcttggc attcatttcc
     5341 cgatggatag ccccaattgc cgcaaaacaa ggcgcacaaa gtaaattgaa ggctaacaat
     5401 gaataagctg ctgcgggagt atatgtgtgt tgtagcactt gccagatttc tctgccattt
     5461 tcggatactt ccgcatgacc aaacaaaata ccaaatgtgc caataatatt ttctttagcg
     5521 accaatcctg taatcgctgc gactgttcct tgccagtttc cccaaccaag cggctgaaaa
     5581 acaactgcca ggattcgccc gaaaaaggct aagatacttt ggtcttcaga aacaggttga
     5641 gcataaaaat tataagaaga gctgaaccag atcacgatac tagaaacaaa gataatcgtt
     5701 cctgcttttt taacaaaaga cttgctgcgg tcataggttt gtcttaggac cgctgaccat
     5761 tgaggaaaat gatatgcggg caattccatg ataaaaggag ctgtatctcc agcaaacaaa
     5821 cgtgtttttt tcaagctgat tccagagaaa ataattgcag tcataccaag aaaataggca
     5881 gaaggagcta cccaagattg atgaggaaaa aaggcccctg ctactaaggc aataatcggc
     5941 aactttgcag aacaaggcat gaaggttgtg accatgatcg tcaaacgacg atcttgctcg
     6001 ttttcaatcg tgcgactggc catgacgcct ggcactccac atcctgtagc aatcagcata
     6061 ggaatgaagg attttcctga aagaccgaat ttcctgaaaa gtcgatccat gacaaaagcg
     6121 atcctcgcca tatatccgca gtcttccaga aagctcaagc ataaaaacaa tataatcaat
     6181 tgcggtagaa atcctaacac tgctcccacg cctgcgatga tgccgttcaa gatcagctcc
     6241 tgcatccatc ccgctacttg ccattcagct aacaatctgc caacatgttc tggtaaccat
     6301 gtaccaaaca atgtgtcatt gatccaatct gtccccattg ttcctactgt ttgaatagcc
     6361 agataataga tcagccacat cacaaatgcg aagatcggca aggctaacca acgattcgtt
     6421 gcaatctgat cgattttgtc actcatcgat aattggaaag aatcatttga cacaacacag
     6481 agtgtacata gttgggtaat aaattcataa cgttcattga ccagaatcgt ctcactatcg
     6541 tcgtccaata gtttctctgt taactggatc agttgctcga tctctttttg ctgagaaact
     6601 gctatctcgt aatcctctct taccttctga tctcgttcaa aatatttcat tgcataccag
     6661 cgtttcttgc cgctttgttc tcctagtaca tcttcaattt catgtaaagc tgcttctaaa
     6721 cgtggatcat atgtacaggg aacaggaata gaaggccgct tttttgcaga ctcctgaatc
     6781 ttttcgatta gtttttcgat tccttgcttt ttcaatacgc tgattggtaa aactggtaca
     6841 ccaagaccat aggaaagctt ttctagattg attttttttc cttctttttt gatgacatcc
     6901 atcatattca gaccaatgat cataggaatg tctaaatcca ataactgcgt agttaaataa
     6961 agatttcgtt ctaaattggt agcatcaata atgttgatga tcagttcagg ctgttcctga
     7021 accaaataat ctcttgcaat cacttcttct ggtgtataag gagacaaaga atagatgccg
     7081 ggtaggtcct gaatatgaat gttcggattt tttttcaacg atccttcttt tttttctact
     7141 gttacgcccg gccaattccc tacatattga ttcgaaccag tcaagagatt gaacaagcta
     7201 gttttcccgc tgtttggatt tccgactaac gcgatattca ttctttttcc tccctttgta
     7261 tcaagattag ttcggcctct gactttcgta acgtcagttc ataccctcgc actgtcagtt
     7321 cgattggatc tcccagaggg gctagttttc ggataactat ttttacattt cgcgtcaatc
     7381 ccatatccat taaccgtcgc ttcactgcgc cttgtccgtg gattgctacg acgatcccag
     7441 tctctcctgc ttttaattga tcaagagaag tccagctttc agaggaagca gtctttttat
     7501 tgacaaagat ttgacctagt atttcccgac ttactgccac tcgatgactt tttaccagta
     7561 aaatggcatt gttgcctgat aaattgacga cgacgatttt acttccggca acgatcccga
     7621 tattttgcaa atggcgtttt gttttttctt ctgtttcgat tcgttcgact tgataaacga
     7681 aattctgctt tgcctgcgct aaggtctgca tattgattcc tctcttgtga aataaatcat
     7741 gtattaaaat cattcattat atgttctaat tttcttacgt gttacgcaaa taaatgagaa
     7801 tcattctcac atagaatact ctcggtaatt gagaatgtca attatacttg tgatattatg
     7861 agcgaatctt tagtattgat agataaaaat gagaaaaaaa tatttcttca tagttttcca
     7921 caattttttc tctattaaat ttgctatttt cccacacgat acttgttttc agctcttttt
     7981 tcaaaaacac aaaacttagt attccacagt ttccacaaca caatatgtag tgtttgttgt
     8041 ttgacttttt tactaagagt tttatactat ctattataga taataagaat gtgttagagg
     8101 aggcactcac catgaacgta aaaatctttt ctaaaaataa ttgtatgcaa tgtaagatgg
     8161 tcaaacggtt tttaacagaa aatcaaattg cttttgaaga agtcaatatc gatgaacagc
     8221 ctgaagcagt agactggttg aaagcacaag gattccaaag tgttcctgtc attacttctg
     8281 atgcagaaac agttatcgga ttccgtcctg atcagttgcg taaacttgcc agctaacatg
     8341 actaaaagaa tcaaaacgtg atacatggat ttgaagggct tgagacagca ccagtttttg
     8401 tcgcaaactt ccaaatcctt ttttaaagct taaaaacaat gcaatgattt gcaatcaagc
     8461 atcatcaaat acgaaatgaa ggttgggcgt acgtatgagt cttaaaaatt tacaagatgt
     8521 cagttatttc aagctgaata atgaaatcaa tcggccagtc aatggtcaaa tccctttgaa
     8581 taaggataaa gaagctttag ttgccttttt tgaggaaaat gtcaaaccaa atacgatgac
     8641 ttttccatct attatggaaa aaatcaatta cttgatcgat caagaatata tagaagcaga
     8701 attcattcaa ttgtattcac catcatttat tgaaaagcta tatcaatttt tggacgagca
     8761 acatttttct ttcaaatctt ttatggctgc ttacaaattt tattctcaat atgcgttgaa
     8821 aaccaacgac ggttccgctt atctggaaac ttatgaagat cgagtagcct tcaatgcgct
     8881 ttactttgcg aacggggatg aagaattagc acttgctctt gcagacgaaa tgatccatca
     8941 acgatatcag ccagctacac cttctttctt gaatgctgga cgtaaacgtc ggggcgaatt
     9001 ggtgtcttgt ttcttgactc aagtcactga cgatatgaac tctatcgggc gctcaatcaa
     9061 ctctgctttg caattgtccc gtattggtgg cggcgtaggg attactttat caaatcttcg
     9121 tgaagccggc gctcctatca aaggatatga gggtgctgca agtggtgtcg ttccagtcat
     9181 gaaattattc gaagatagtt tcagctattc taatcaatta ggtcagcgtc aaggagctgg
     9241 cgtggtttat ttgaacgttt tccatccaga cattattgct ttcctttcag caaaaaaaga
     9301 aaatgcagat gaaaagattc gcgtaaaaac tctttctctt ggcgtgatcg tacctgataa
     9361 attttatgaa ttgacacgta aaaacgaaga tatgtattta tttagtccat atagtgtcga
     9421 aaaagaatat ggtgtcccat tctcttatgt aaatattaca gctgagtatg atcatctagt
     9481 tgcaaatcca aacattcgca aacaaaaaat caaagcgcgt gatcttgaaa atgaaatctc
     9541 aaaattacaa caagaatctg gctatcctta cgtaatcaat attgatacag cgaaccgcga
     9601 gaatccaatc gatggcaaga tcatcatgag taatttatgt tcagagattt tacaagttca
     9661 aacgccttct gtgattaacg gcaaacagga atatgaagta ttaggaacag atatctcatg
     9721 taacttaggc tcgacaaata tcgtgaattt gatgcaaagc cctaactttg gtcaatctgt
     9781 gcgtacgatg actcgtgcat tgacatttat tacagatgca tctgatatcg atgtcgtacc
     9841 accgatccaa aacggaaata agctgaatca tacgattgga ttaggtgcca tgggcttaca
     9901 cacttatctt gcaaaagaac aaatggaata tggctcagag gattcattag actttacaaa
     9961 tatttacttc atgctgttga attattggac acttgttgaa agcaacaata tcgctcgtga
    10021 aagaaaacaa gttttccata attttgaaaa atcagcttat gctgacggca cttatttcga
    10081 aaaatacgta actggagaat tccagccaaa atcagaaaaa gtcaaagcat tgtttgaagg
    10141 catttttatc ccaactgctg aagattggaa tgctttgaaa caagcagtaa tgaaagatgg
    10201 actgtatcat caaaatcgct tggcagtagc accaaatggc tcaatttcat atatcaacga
    10261 tacgagtgca agtattcatc ctatcactcg catgatcgaa gaacgccaag agaagaaaat
    10321 cggtaaaatt tattacccag cgccttatct atccaatgag acgattccgt attacacttc
    10381 tgcttatgat atggacatgc gtaaagtaat cgatgtttat gcaacggcac aacaacacgt
    10441 cgatcaaggc atgagtttga ctttattcat gcgttctgaa atcccagaag gcatttatga
    10501 atggaagaca acaacgaagc aaacgacacg tgatttgaat attttaagac attacgcttt
    10561 ccacaaagga atcaaatcca tttattacgt acgtaccttt actgatgatg cagaagaaat
    10621 cggaagcaat caatgtgaaa gctgcgtgat ctaatatcat agatagattc gcttattcat
    10681 attttaatta aaggagcatt ctcatgtcag aaacatatta tgaagcaata aactggaatg
    10741 aaattgaaga tatcattgat aaatccactt gggagaagct gacagagcaa ttttggctag
    10801 atacacgtat cccactttca aatgacctag atgattggcg tacattgtct caattggaaa
    10861 aagatacagt aggacatgta tttggcggtt tgacactgct agatactgtc caatctgaaa
    10921 gtggcatgga tcagttgcgt aaagatgtcc gcactcccca tgaagaagcc gttttgaaca
    10981 atattcaatt catggaatcc gttcatgcca aaagttattc ttctattttt agtacactga
    11041 atacgaaaaa agaaatcgaa gaaatttttg actggacaaa tacgaatcct tatctgcaaa
    11101 aaaaagctga gcgtatcaat gaaatctaca aacacggtac accgctagaa aagaaaattg
    11161 ccagcgtctt tttagaaaca ttcttgttct attcaggttt ttatactcct ctttattatt
    11221 taggaaacaa caaattggct aacgtagcag agatcatcaa actaatcatc cgagatgaat
    11281 ctgtacatgg aacctacatc ggttacaaat tccaattagg attcaacgag ttacctgaag
    11341 cagaacaaga aacgttaaaa gactggatgt acaatcttct ttatgagctt tacgaaaacg
    11401 aagaacgtta tacagaagaa ttatacgatc caatcggctg gacagaagaa gtcaagacat
    11461 tcctacgcta taatgccaac aaagcattga tgaacctagg gatggatcca ctctttccag
    11521 atactgccaa tgatgtcaac ccaatcgtaa tgaacgggat ttcaacaggc acaagcaacc
    11581 atgacttctt ctcacaagtc ggaaacggct atttgttagg tcatgtagaa gcgatgaaag
    11641 acgacgatta tttgattgga ttggaataag gatttatttt aattcaaaaa tttttcgaaa
    11701 aataaaaaga gtttagcgta cagatatttt aaatgtacac tgaactcttt tttatgctta
    11761 ttcacttgtt tcttccagca cttttggact gtctaacttt tccgaaacat gttccattct
    11821 tctcaataaa tagattttcc ggatatttaa tattatcgtc ttaatcactg catagacagg
    11881 tatgcaaatc agcatgccta atattcctgc gacacttcct gctccaatca ataccaagat
    11941 gatcgtcaaa ggatggatat tcattgattt cccaaataac aacggcttga tcaaatttcc
    12001 atctaattgt tggatcacta aaatcgacaa cgccatatac aaagcttgaa atggagagat
    12061 aaacaaaccg acaatgattg ctggtaccgc tccaataaaa gggccgacat acgggataat
    12121 attcgtgatt ccgcaaatga ccgcaagcaa caagctataa ggctgatcga atattctcat
    12181 tgccaaaaag ctcattacac caataatcaa agcatctaag atcgtactgc tgatataggc
    12241 agataatgtt tcgtttaatt cttttaccgt ttggcgtaac tctttacgaa tcgctttcgg
    12301 aaagaagtga gacaacgcat caataaattt gtgcccatcc ttgaacataa atagcaaaat
    12361 aaatggaacg gtaaataaca aaataaaaaa ttgcatcata aatgaaatga tttttgagac
    12421 acttgcagat aaactgacta atatgatatt gaacaaatta ctaagagata aatttgctga
    12481 tgctaattgt tcttccaaat tgaaattttt aaactcttcc cgctgcgcca attcatttaa
    12541 ccatttgctt gtttcatctg catacattgg caagctctgg gtcaattgaa ttgtctgttc
    12601 cattaactga ggcactacat tcatgacagt taaaacaact aaaatgatta cgaccaaaaa
    12661 cgtcaataaa aaaccgaaca ctctcgggat ccgatgcttt tcgagaaaaa caacgattgg
    12721 atcgaacata taatagataa aaccagcaac aagcaatggt aaaaaaattg ccgaaagcat
    12781 tttcaatatt ggactaaaaa tattcggcat ttgtaaaata aagtacacgc cgatgatcgt
    12841 gagtactagc tccactgtcc agaaaaataa ttttgattgt ttaaaacgtg attgcataca
    12901 tctcctccaa ggtcctttga tacttctatt ctatcattta acaaagagtt acgcgctgag
    12961 attttcattt gcttaaaaaa aacaaaaaaa ataaacatat tttaatgagt agacgagtac
    13021 ttaaacaaaa acacggtttg gagtagtgac aaaaattata ctgcagtccc tttttccaaa
    13081 taaacggtgt tcaaaagctt gcatcttcgt aataagccca gccaaacgaa aaatataagg
    13141 aactattttt atttggctgg gcttattact tgatgtagga catctatttt acaacctcag
    13201 atcatttatt gcgcaatact gactaaatgc aactttgtga tatttttgtg tacggacaac
    13261 tcctcgatca catcggcata agtgagccct tttggaagat caatcgtata gatatttttg
    13321 tacactcggt agtcatcaac gaattttacg tcaaaattca catcttcgat tacgatattt
    13381 ttctcttcaa aataatcggt aagaaaatct ttggtttctt ttctatggat aaattggatt
    13441 tctaactttt tagtcgtagg tacatggatc acgcgtttca ctactgtgag tgcaatagca
    13501 atcccgataa aactgatgat cgcaatggaa tagtaaccca tgccgatggc gatccctaat
    13561 ccagcaattg cccataaaga agcggcagtc gtaagtcccg tcaccgattg tttcgtgacg
    13621 atgatcgttc ccgcgcctaa gaaaccgaca ccactcacca cttgagcaat cagccttgct
    13681 tcatctgaac gaatcgttcc agcaagctgt ggatactcta aagcatctct taatgcacca
    13741 ctagcgatct caacttggat caatgcaatg atacatgctc ccatacaaac taaaatatgc
    13801 gtacgcatac ctgctggacg atttttatat tgtcgttcaa aaccgattgc tccgccaaat
    13861 agcatagcga tccctaaacg taaaataatc tctggaattg tcaataccat atcattcatc
    13921 taaacaccct ccagtccaat atatcatgtc tgaaaggagc agacaaaaaa agtgcaattt
    13981 ttacctctta cgaaaaaagg actgtgacaa aatttttgtc atagtccttt ttatgaataa
    14041 acggtgttca atcagctgac cctcggtatt actcaacaaa cggcaaaata tggggagcta
    14101 tttttccttt gtcttctcta atactcaggt caaaacgctg atttcacaac ctctttagcc
    14161 tactattgaa aattacaact tacttgctgc ttcttcatcg atgatcacaa caacattgtc
    14221 gtgattttgc aatacacttg ctggcaaatg attcgttaca ggtccttcaa tcatcccttt
    14281 gattgcatca gctttgcttt cgccaaatgc catcaagacg atttctttac ttttcataat
    14341 tgaaccgatt cccattgaaa cagcttttgt tggtacatct tctgctttat cgaagaatcg
    14401 tttgtttgca ttgattgttg attctgttaa gtcaactact gaagtcgttc cgtcaaatga
    14461 agcaccaggt tcattgaaac cgatatgtcc attttgcccg attcctaaga tttggatatc
    14521 gattggatgt gcatcgatga tttgatcata ctttttgcat gctgcttcca gatcttctgc
    14581 ttttccatct ggtacaaacg tttctttgaa tggtttttta tcaaataatt ggacatccat
    14641 gaaatgacgg tagctttgtt catctgaacc gcctagtcca acgtactcat ctaagtttac
    14701 agaagtcatt tcactgaaat ccaaatcgct tgaagtcatt tctttatata aagtaactgg
    14761 tgtgcttcct gttgccaatc ctaatacttt tgcacctttg ttcatacctt ctttgatgat
    14821 ttcaaatgct ttttttccgc cttcttcagc gttttttaca cgaatgattt ccatagtgat
    14881 tgctccttta tattttggta tagcccaatt atagcattct ttttttcttt ttacaataat
    14941 tagtatagac caaattgcat ttttttcatt ttcttgacaa taaataggaa tccgtgttag
    15001 gtttcataag atggttcaat caaacaatcc atgttgcatg aaaaccaagc aagtgattca
    15061 ctgtggacgc tttatgtctg cagttttttt atgaaaaagg agttattatg agtatactga
    15121 cattaaacaa cattacccag caatttggcg acaaaatcct ctatgaaggt gtcgatctgc
    15181 aattaaatgc tggcgaacat cttgggctga ttggacaaaa cggagccggt aaaagtacat
    15241 tgattaaaat catcactggt gagatcttac cagatgacgg acagatccat tggcaaaaaa
    15301 atattcacaa aggttacctc gatcaatatg tggaagtcga tgaaacatta acaatcgaag
    15361 aatttctaaa aactgcgtac gcaaaagaat acgaaaagga agaaaaaatt gcttttcttt
    15421 accaaacata tagcgagacc ttcgatgatt ccttgctgga aaaagctggg aaactccaaa
    15481 cggaattaga ccaaggagtg ttttatcaaa tcgacacctt agttgctgag atgagcagcg
    15541 ggttggggat cgatgtctta ggtttagatt cactgttgaa aaatctaagt ggtggacaac
    15601 gttcaaaggt gattttagca aaactacttt tagaaaaccc agatgttctg atcttagatg
    15661 aaccaaccaa tcacttagat gatcagcata tccaatggct gacgcagttt cttcaggatt
    15721 ttgacggtgc gtatatcgtg atttcccatg accaagaatt tcttgatcag atcaccaccc
    15781 atatcgcaga catcgagttt ggaaagatta ctaagtatac tggccatcta aaacaagcat
    15841 tgaaacaaaa ggaacaaaat cgcgaaagct atttgcgcca atatcatgct caacaaaaac
    15901 aaatcgaaaa gaccgaagct tatattcgaa aatacaaagc cggttcccga tcaacgatgg
    15961 caaaaagccg acaaaaacaa ttagataaaa tcgaacggct gacgcctcca gcttcagcaa
    16021 gtaaagctgt ctttgatttt ccattctcgc ctattgtcac gacattagca gtggaaacaa
    16081 ctgatttagt catcggatat gatcgaccgc tacttgcacc catcaatttg accatccgct
    16141 ttggtgaaaa tattgccatt cgaggcttca acgggattgg aaaatctact ttattaaaaa
    16201 cactgatcgg tgagattgaa aaaataagtg gcgatttcca cttcccagat aacacaaaaa
    16261 tcaattactt ttcacaagat ctgtactggg aaaatccatt agaaacacca ctacaatatt
    16321 taagcagtca atttccaaaa gcgactatca aagaattacg tcgccaacta gctaaagcag
    16381 ggttagttaa tcaattagca agtgaacctc tcactacatt aagtggtggg gaacaaacta
    16441 aggtgaaact tgcacagttg acaatgaacc aaggtaattt actgattctc gatgaaccga
    16501 cgaaccatat cgatcaagaa acgaaagaaa gcttacaaga cgggatacag aaatttccag
    16561 gaacagtaat catcgtctct catgaacaag aattttatca agatcttgtg caacgagtca
    16621 ttgagattga accaaaataa acgtttgatt gaatagttga aaaaaaggag ctgtgacaaa
    16681 agttttgtca caactccttc ttctgaataa atggtggcca attagctgac cttcggtatt
    16741 agctcaaaaa acggcaaaat atgaggagct atttttattt ggctattctt attacttgat
    16801 gcagaacagc ttttcacaac ctcttttcgt ctatttttta ttttcgagat ttctctttcc
    16861 tttaaaaaca actgtttgaa agaagaacgc acatccttga aaaacaagcc cgctaaccgt
    16921 tacacctaac cagccatact gctgccacat tgtcgtccct agcaatgaac cagccgcacc
    16981 tccaataaag tacataaaca taaatacaga cccgtggtgc tagttaattt taatatccta
    17041 aagaatatct caaagttcct tcaaaacgtc gttgtgctcg acttaataga acactgtaag
    17101 ccagtaatat gctttcaaat ctactctcta gcc
//
LOCUS       DACDIH010000056        16884 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDIH010000056 DACDIH010000000
VERSION     DACDIH010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.056"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(258..1763)
                     /locus_tag="IV281_001977"
     CDS             complement(258..1763)
                     /locus_tag="IV281_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8780959.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1760..2446)
                     /locus_tag="IV281_001978"
     CDS             complement(1760..2446)
                     /locus_tag="IV281_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8780960.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2639..4060)
                     /gene="gndA"
                     /locus_tag="IV281_001979"
     CDS             complement(2639..4060)
                     /gene="gndA"
                     /locus_tag="IV281_001979"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP8780961.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4230..4409)
                     /gene="rpmF"
                     /locus_tag="IV281_001980"
     CDS             complement(4230..4409)
                     /gene="rpmF"
                     /locus_tag="IV281_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8780962.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4467..5036)
                     /locus_tag="IV281_001981"
     CDS             complement(4467..5036)
                     /locus_tag="IV281_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP8780963.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5222..6124
                     /locus_tag="IV281_001982"
     CDS             5222..6124
                     /locus_tag="IV281_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8780964.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(6159..7943)
                     /gene="pyk"
                     /locus_tag="IV281_001983"
     CDS             complement(6159..7943)
                     /gene="pyk"
                     /locus_tag="IV281_001983"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP8780965.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(8017..8979)
                     /gene="pfkA"
                     /locus_tag="IV281_001984"
     CDS             complement(8017..8979)
                     /gene="pfkA"
                     /locus_tag="IV281_001984"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP8780966.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(9147..12461)
                     /gene="dnaE"
                     /locus_tag="IV281_001985"
     CDS             complement(9147..12461)
                     /gene="dnaE"
                     /locus_tag="IV281_001985"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP8780967.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12692..12877
                     /locus_tag="IV281_001986"
     CDS             12692..12877
                     /locus_tag="IV281_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP8780968.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(12939..14135)
                     /locus_tag="IV281_001987"
     CDS             complement(12939..14135)
                     /locus_tag="IV281_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8780969.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(14305..15741)
                     /locus_tag="IV281_001988"
     CDS             complement(14305..15741)
                     /locus_tag="IV281_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8780970.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            16034..16843
                     /locus_tag="IV281_001989"
     CDS             16034..16843
                     /locus_tag="IV281_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8780971.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         4954 a         3539 c         2950 g         5441 t
ORIGIN      
        1 aaataataat atagcaatct ctttataaaa ttgcaataga aaattcccta tttttattta
       61 ttatttttaa acacgaatat tacagcattt tattgttttt tcgttcatct aaaagatact
      121 tacatttaat taacggatgt tattctattt aattgttcgc tagttttttt ataatgacat
      181 ccctgaattt ctcacttgct tgtattttgt accttattct ttgttggata tataaaaaaa
      241 caagtaaagc acatgcttta cttgtcttct gcctttgcga taggaataaa gattctaaaa
      301 attgtccctt gccctactac actttctgct aagatacgtc ctttatagtt ttcaaccagt
      361 tgtttagcga tagacaaccc cagaccattt ccacccttcg ttctagctct tgctttatcc
      421 actcgataaa aacgattgaa gatcttatta aggtcttctt tgggtattcc ttcgccaaag
      481 tcctgaatcg cgatctcaaa ttcattcaga gtggaagaaa tagagatatg gacttctttt
      541 cgtgtagttg aatactttac tgcgttatcc aatatgataa tgattaattg ttcaaagtga
      601 ttacgataaa tttgcagggt cacttctttt gtcaaatcat catctaaagt gatcacatag
      661 tctggatata atatcttgaa attattataa acttgatagg tgacttcttt tcctttagtt
      721 gtttcattcg cataatgaac atctacttgt tctgctcttg ataagtctag catctcttga
      781 actaagcttt tcatacggct gatttcttgc atgctggcgc ttaatgattc ttctaatatc
      841 tcaggatcat ctttccccca acgatttagc agactaagat gcccttcaat aattgcaaca
      901 ggtgtcctta actcatgaga tacgtcttcc acgaattgct cttgttgttc aatatacaaa
      961 cgcatacggt caagcatctc attgaatatt tcagctaaat ctgccaattc atcatttgtg
     1021 tcgatttcag gcatgtggat atctgattgc ggatctttac gtatagtatc cattgtatct
     1081 ctgagtacct tgagtggttt taagaaataa gaagataaaa agaaacctaa aatactactt
     1141 aagatcaaag aaacaacttc taaaacaata agagtcaata atagcttatt tcgaatatca
     1201 tagaaagaag ataattcata gaagctttgc gcatagccga tcttttctct tgtaccttta
     1261 gaatatatag gctggataga taaaaagcca gtttttcctt ctagtgtaac gatcgttgga
     1321 actttacgtg tagtctgaat caggtttcga ctgttttcat gggttttaaa aatcaagttc
     1381 tcgtccaaat cataaacata aagatccatt gatggctgcc ctagctcagc tataaaggaa
     1441 tcgatcttca ttaagtttcc ttcgaccagt gaagtatgat ctacgacctc atctccgtca
     1501 tttgtcgctt tcgtcagatt acgatacgta tttactaatg tcagttcttc atccgagtta
     1561 gccaaacgag aagctacttc tgaaatcgta cgctctacat tgtttctttc ttttgccaca
     1621 atcagattga tagatgattt ataggtaatg acagcgaaaa ttgtaaacac aacgaatatg
     1681 aagaaagaac tcgcaaaggc ccacttgatt gtcaaggagg gccctttcag ttctcttttt
     1741 tccgattttt tcggctttat cacgagcgca tcacataacc tgttccgcgt acagtttgga
     1801 tatagctttc ctccccggga acgtcaattt tgttgcgaag ataacgaata tatacgtcca
     1861 ctacgtttgt ttcaacttct gtttcgtatc cccacacttt atttagtaaa acgtcacgtg
     1921 ctaataccac gttcacgttt tccattaaag taagcagcaa ctcgtattct ctttttgtta
     1981 gttcaattac ttcatttccg cgacgtacga cacggttttc tttttcgata gtcaaatcgc
     2041 gataagtaat agtagtttgt ttagccacat ttttatcgcc ttcaatatcg attcgacgca
     2101 acaatgcacg aagacgtgct aataattctt cgatagcaaa tggtttgacg atgtagtcat
     2161 ctgctccgtg gtcaagtcca gatacacgat cgatcacaga atctcttgca gtcatcataa
     2221 tgattggtgt atttttgact tggcgtacac gtctgcagac ttctaaaccg ttcaattctg
     2281 ggagcatcag atccaacaat atggcatccc actcattatt taatgccgca tctaaaccag
     2341 ttcggccgtt gtagtggact tctgcgttat acccttcatg cttcaactct agctcgacaa
     2401 atcgtgccaa gttcttttca tcttcaatga ttagaatatt gctcatttat tattttatcc
     2461 tttctattct actggctttt taaaaactct atcattatac cgataatatg cttaaaaatc
     2521 tatagtcatt ttattatgga tagcaatttt aaaaaagagc tgaagtctac tcataacaat
     2581 cgacttcagc tctttcgtcc tctttttatt ttctcatttc ctaacagccg aaagactatt
     2641 attcttcatg gtaccatgag taatggaaga ttccttcttt gtcgacacgc tcataggtat
     2701 gtgcgccaaa gtaatcgcgt tgggcttgga ttatatttgc aggcagacgt tctgctctgt
     2761 atgaatcgaa gtaagcgatg gctgatgaga aagtcggtac aggtacgcca gcttggacag
     2821 ccaaggcaac gacgtctctg actgattgct gatatttttt cgtgatttca acgaagtaat
     2881 catctagcaa taaattttcg atctcaggat ttttgtcata ggcatccgta attttttgca
     2941 agaatcgagc tcggatgata catccagcgc gccagatttt tgcgatttca ccaaatggca
     3001 agtcccaatc aaattctttt gaagcgacac gtaattgagc aaatccttgg gcataactca
     3061 tgattttact gaaatacaac gcttctcgga ttttttcgac caattctttt ttgtctcccg
     3121 tgaactcaaa atcgttcgta cgagacaaaa tcttgcttgc ttgtacgcgt tcttctttat
     3181 aagcagaaat atagcgggca aatacagatt cagtgatcaa tggaagtggc acacctaagt
     3241 caagcgcgct ttggcttgtc catttacctg ttcctttatt cccagctgca tcaaggatca
     3301 catctactac tggtttacct gttccttcat cgtctttacg tgtcagaatg tcagcagtga
     3361 tttctatcaa atagctgtct aattcgcctt gattccattc tttaaagata tcagccattt
     3421 cttctactga aaggtttaaa atattcttca ttaagtcata agattcagca atcagttgca
     3481 tatctccata ctcaataccg ttgtgtacca ttttcacata atgtccggct ccatttgggc
     3541 caatatatgt gacacatggt gctccgtctt ctgcttttgc agaaatttgc tctaagattg
     3601 gtgcaactag cgcataggct tctttttgtc caccaggcat gattgaaggt ccttttaaag
     3661 ctccttcttc accaccggaa acgcctgtac cgataaagtt gatgccagag ttggctaact
     3721 cttcattacg acgcatcgta tctttaaaga atgtatttcc tccgtcaatc aaaatatcgc
     3781 ctttatcaag gtgaggaagt aactcttgga ttgttgcatc tgttgccggt cctgctttga
     3841 ccattagcaa gatccgacgt ggtttctcga tagattctac aaattcttcg attgtatatg
     3901 ttgctttgaa atttttatct gggtgttctt ctactacagc ggttgtttta gcccctgtac
     3961 gattgaataa agccactgaa tagccgcggc tttctatgtt caaggctaga tttttaccca
     4021 taacggccat accgacaacg ccgatttgtt gttttgacat atgaactcct cctacatacg
     4081 ggatttctcc ctaatctctt acattatagc ttaacacggc tacattgcaa actaaagaac
     4141 aacatatttt ttatttaccg actacaaaaa aataaacctc aaggctccat ggttagcctt
     4201 gaggttttac attttctaat taaaatgtat taagcttctt ttgacattac gtccttacca
     4261 tcatagtaac cacaatttgc acatacgtga tggctttttt tcatttcacc acagtttgga
     4321 cattcgttta accctttgat tgtcaatttg tagtgtgtac gacgttttgc ttttttagct
     4381 tttgatgttc ttctagctgg tactgccatt ttgctacacc tccttgtaaa gtcttgtatc
     4441 taaattagac acaatatatt ccaatcttac ttgttatcgt cgtcttttga tttagaatca
     4501 aaaagttcag atagttttgc aagacgagga tcaattttat tctcggcttc ttccattttt
     4561 ctgcgctggt attcttcttc ggaaacaact tcccaatcat tgccttttgg aagttctgtt
     4621 gctgcttcct cttcttttgt caatacccgc attggaatcg ccaataagat gttgtcttca
     4681 atcgactctg ctaaattcaa taatggacct tcaattagca aaatctcatc tgcagacatt
     4741 tgttcatctc tactttggaa ttgttcttct gtcatgaata cttcgtctac agaaaaatcc
     4801 atgggcagtt caacaggttc tagtgaacgt gttgaaggaa ctgtcaaagt cgtctgcaca
     4861 gtgtaatgaa cgatatagcc tttcttatca actgagacca aaccttcgac ttccactggc
     4921 gcaacatcaa gtattaaatt gtctcgcttc atgagagcgg ctttcaagtc gagcgtctca
     4981 tgaaaagtta acggtgtctc ctggtatttt ctcaattcca ataaagacca tttcattttt
     5041 catcactcct aagcaacaaa agcaattata cagggcaaaa agcatgttgt caatgaaatt
     5101 ttcttgacac cttgcagttc ataaaaaaac agaaaaatct ttattagaaa gcctctgttt
     5161 gctatcatcc tcagaatcat ttacaataat cattattcca ttaaaggagc ggatgatctt
     5221 catgaacacg aaagatatcg aatactatgt ccaaatcgtc aaacaaaaaa attttacgaa
     5281 agtggctaat tatttccgtg tgagccaacc tactattaca tatgcattac aacgtcttga
     5341 aaaagagttt gacaccgtac ttattacaag agatcgatcg catcacgaat tgatcgtcac
     5401 gccttctgga aagcagctgt ttctccatgc acaaaccatt ctccaagaga tttctactgc
     5461 aagagctgaa atcactcagt tgcaaaagaa aaagcttcgt tttggcatgc ctcctattat
     5521 cggcaatgaa tactttccta aactgtcaag ttactttatc aagcatgaat tgatgaatca
     5581 aattgagatt accgatggag gctctcggga cttatacggg atgatccgac aagggaggat
     5641 cgatttagct gtattaggct caacacagcc aatcgtggat caatatttag aaacagagtt
     5701 acttttggac aaacgtttca tgatcgtcgt gccacctaca catcctcttt ccaagcagga
     5761 tactgtctct ttcaaagata tagcagatga acagtttgta ctattgaacg aacattatgt
     5821 ccaccccgca gcattcacta agctttctaa acaaacacat actcatccaa aagttgttta
     5881 tcaaagcaat gacttgacga ttttaaagag tatggtccga gaaaatgtgg gtatcggttt
     5941 tttggcagag attgctgtcc atcctgaaga tcatttagtt tgcctccctc tttcagatga
     6001 agttcagcct cgcttcttga tttcattggc aaaacgaaca cagcagatcg tcacgcctct
     6061 tcaagaacaa gtagtccaag ttatccatga attcgttaca gcacaaaaaa acagctcttc
     6121 gtaaaagaag agctgttttt attttttatt aagctaaatt agatagctgt tgtttcgcca
     6181 cggtaaacga tgccgcgacg tgaatcaacg gtaatcaatt caccttctgt aattgttgtt
     6241 gttgcgcctt tagcaccaac gattacagga atatcttgtg caattcctac aactgcagcg
     6301 tgagaagtca atccgccttc ttcaacgata acagcagctg ctttttcgat tgcaggcata
     6361 tattctttgt cagttgatgg tacaactaga accattccgt cttttgcttt ttttacagct
     6421 tcttcagcag atgaagcaat aactgcattt gcaacgacag atttttgtcc aacgccttga
     6481 gcttctaata atttagaacc aatcatttga attttcatca cgttagttgt tccgcgttca
     6541 ccaactggta caccagcagt gatcaagatt aaatcgcctt ctgatgcgaa acctaattca
     6601 acagcttttt tagctgctag ttcaaacatt tcatcagttg ttgatggttt ttcagcaact
     6661 gttgggtaaa cgccccagtt taacatcaaa ccacgttttg tgcgttcgtc aaaagtaaca
     6721 gccaagatat ctgcatctgg acgatatttt gaaatcattt ttgcagtgta tcctgattca
     6781 gttgcagcaa cgattgtttt cacgcctaga ttttttgcag cacgagctac agataaaccg
     6841 attgtttcag ttacatctgt tttatcgaat tcgttcaatt ggaatgtacc taattctgat
     6901 aatgcatttt cagcttcttg gtcgatacgt gccattgttg aaacagcttc aactgggtag
     6961 tccccgtttg cagattcacc agatagcatt gttgcatctg tgccatcaaa tacagcgtta
     7021 gcaacgtcag aagcttccgc acgtgttgga cgtgggtttt cttgcattga ttctaacatt
     7081 tgtgtagcag tgataactgg gataccagca gcattacatt ttttgataat gcgtttttga
     7141 accattggta ctagttcagc tgggatttca acacccatat ctccgcgggc aatcataaca
     7201 ccgtcagaaa ctttgatgat ttcgtcaatg ttgtcgatac cttcttgaga ttcgatttta
     7261 gggaagattt gaacatgagt catgtctttt tcttctaaga tttcgcggat atctagaacg
     7321 tcttgtgctt tacgtacaaa acttgcagcg ataaagtcga tatcgttgtc taaaccaaaa
     7381 cgaatatcgt cagcatcttt tggagtaata cctggcaaac taatagaaac gcctggtgca
     7441 tttacccctt tacgtgatcc tagcatacct gcatttttaa cttctacgac taattcacgg
     7501 tttgcttcgt ctttttcaat gatttgcata tcgatcaaac catcatcaaa taaaatgtgt
     7561 ccgccaacgt gaacatcgtc aaataatcct gggtaagtaa ccgcgatttt ttcttttgaa
     7621 cctacatgtt caggatccat agagatgcgt gtttgatctc ctacttcaaa tttgatgtat
     7681 cctgcacgac caaaatcagc ttctgttgtg ccttgaacag ttgtacggat ttcagctcct
     7741 tttgtatcta aaagaatacc gacatctttt cctgtctttt taactgcttc acggaccata
     7801 gtcatacgtg ataattgttc ttcatggtca ccatgagaaa aattaaagcg gaagacattt
     7861 gccccagatt caatcaattg agaaataatt tctactgagt tacttgctgg tcctaatgta
     7921 ctaacgattt tggttttttt catttaaaaa actctcccat tcaattttgt ttatttaaag
     7981 ctgtacacag cttaaatagc ttggtttctt tcactcttaa aatgaaagtg aattatttag
     8041 atcatatagt gataagtctg gtttatgttt gttgttttca agtgtatcaa tgatgtcaga
     8101 cgcaacgatt tcgttgtcat ggataccgac acataatcca ccttttcctt cttgaagcaa
     8161 ttcgacagcg taagcaccga atttacttgc caacacgcgg tcgcgtgcgc ttggtgctcc
     8221 accacggaca acgtgtccca agatagatac acgtgtatga tagtcaccaa attctgaaag
     8281 tttctcagcg aattcatttc cgcccattac accttcagcc aagatgatca agcaatgttt
     8341 cttcccgcga tcgcggcctt cttggatttt tttagcaact gaagccatat caaaatcatg
     8401 ttctgggatg atgatttcat ccgctccgcc agccacacct gaccataaag cgatgtctcc
     8461 tgcattgcgt cccattactt cgataacgaa tgtacgaaca tgagaagtcg ctgtgtcacg
     8521 aatacggtca attgattcta gtactgtatt gattgctgta tcaaaaccga ttgtgaaatc
     8581 agtcccagga atatcattat cgattgttcc aggaatacct actgctggga aaccgcgttt
     8641 tgtcaaagcc attgcgccat gataagagcc gtctcctccg ataaccacta gaccttcgat
     8701 tccgaatttt ttcaattgtt cgattccttt taattgacct tcttcagttg caaattctgg
     8761 ataacgtgca gaatacaaga atgtaccacc acgttggatc ttgtcaccta catctgccac
     8821 atcaagacga cgaatatctc cagcaactaa accggcaaaa ccataattga tcccgtatac
     8881 ttccagtcct tcgtaaatac ctttacgaac aacggcacga acagctgcat tcattccagg
     8941 tgcgtctccg ccactggtca aaattccgat gcgtttcata ataaacattc acctcattaa
     9001 aatactttat cctaagtatg acttaggttt acaaccattt ttgcgttaac cacgactaac
     9061 attctaacat tatttttctc acttgtcttg atttacggat aaaaaaatga aaaaaaatga
     9121 aagcaagtag tattttttac cgtaagttat ttgaaaacca cattatcttc gccaagcaga
     9181 taaataagtt gccgctttat actcttgctg tcatccaccc aataagcatc tgacagaact
     9241 acttttcgtt gcgttttttc gtgatacata actactggta tatatcctgg gtgtttctga
     9301 aaaatcactt gtaattgttt cattatctcg ggatcttccc gttcttctgt aatacgcaaa
     9361 tagcaagttt gtgaaggata ctgggcttct aactggttag gatcagcaaa gctttctcca
     9421 attagttgca gttcctgatt atatttgctg atttcgctcc gcccttgaac aaagatcact
     9481 cgatcaacgg caaacaaatt acgatattta cgataaagag ttggaaaaac tgtgacagaa
     9541 atttctccac tactgtcggt tccttcaaca aaggccatca gttctccttt ttttgtccga
     9601 atttctctaa cttccttgat catcactaat aaattgactg gttgattagg gacgatatct
     9661 gtaatcagct gaacttgttt agctaaacga agacgatcaa acccttctgc aggatgacct
     9721 gacaaataaa tacctaagta ctcctcttcc agcttcaagc gttcttctag tgtatagtca
     9781 gcgacttctt cttcttttaa tgccatgatt cccagcaagt caagacttcc accactgtaa
     9841 acgatatttt ggatttttcc ttcgagatca agcatcaatt gtttcctgtt aggagataat
     9901 tcatcaaaaa ctcctactgc tgtcagtgat tgcagtaact ctaatttcag ccaacgctta
     9961 tcgatccgta tgaggaattg atccactgac tggaacgggc cattttgttt tctttcgttt
    10021 aatatatctt ctacaaaatc acgccgaata ccttttatcg ctgtcagtcc gaatctgatt
    10081 tcttccttat tgaccaactc aaaactggaa ttactttgat taatggacgg cgaggaaagt
    10141 ttgagtttat ttttacgtgc ttctgcaata tattctttca attttgatgt gttatgccga
    10201 acagaattca tcaggctggt aaagaaagca cctggatgat gggcttttag atacgccatt
    10261 tggaatccca cgaatgaata agcaaatgca tgtgaccgat tgaatccata gtttgcaaat
    10321 ttttcgatat attcatatac ggtcgctgct gttttttccg aatatccctg ttgtaatgct
    10381 cctgatacaa aatgcgactt ttcttcatct aatacgtctt ttttcttttt actcactgcc
    10441 cgtcgcagaa tatctgactg ccctaatgta aatccagcca tagtcgaggc gatctgcatg
    10501 atttgttcct gatagacgat cactccatat gtgtttttca aaatcgggat cagtgaatca
    10561 tctgggtagc gaatggcttc ttttcctttc ttcctggcaa tgaaggtatc tatattttgc
    10621 atagggcctg gtcgatacaa tgcattgact gcggcaatat cttcgatgtt ttctggatct
    10681 aatcttcgta aaacgttccg gataccagca gattcaaatt gaaatactcc tgaggtttcg
    10741 cctcgttgaa acaagagcag cgtttttgca tcatttagcg ggatctcttt taatcggatc
    10801 tcataccctt ctactcgctt aacagctttc aaagtaaaat caataatcga taagttgcgt
    10861 agtcctaaaa agtccatttt caaaagaccg attgcttcta cctcattcat cgtatactgg
    10921 gtcagaaaag cttcatcaga accgcgttgg agcggaacta gttcaagtag attcttatcg
    10981 ctgataacta cacctgctgc gtgagtcgaa acatgtcttg gcaatccttc taaaatcact
    11041 gcagttttga ataaaagttg gtttttttcg cttgcctgaa ctaattctac taattgatgc
    11101 gaagttttat aagcttgggt caatgtgatt ttcaattgat tgggtattgc ttttgaccag
    11161 cggtttgctt cactttgcga taaaccaaac actctagctg catctctaag aaccattttt
    11221 gctgccattg tgccaaatgt tgcgatttgt gccatatgat attgtccata cttttctcga
    11281 acataattca atacttcttc acgtcgattg tcaggaatat ccaaatcgat atctggcatc
    11341 gtaaagcgtt cggggtttaa aaagcgttcg aacagcaaat catattctaa tggatccaca
    11401 tctgtgattt ctaacacata ggctactaat gaacccgcag cagaaccacg acctgcaccg
    11461 gtaacgattt tgtttctatg agcaaaagcc attacatccc atacgatcaa gaaataatca
    11521 tcgaatccca tttgatggat gacagataac tcgtagtcca aacgttcttg gtattccttt
    11581 gtatagtcag tgattcggcg ttccacttgc gaatagcata actctcttaa atattctcca
    11641 gcttgttttc cattaggaac tggataatgc ggaagtaatt tctgatggaa ctggaattca
    11701 aatgaaatcg attcagctaa acgctgggcg cctattacag cttgggaaag acccgctttt
    11761 tcgaatcgtt tgatcatttc ttgttctgaa agaagataga catttgctgt tagtggtaaa
    11821 gatgcagata aattttctaa aggtatttgg cttccctctt tgatatgttt catcacttca
    11881 agtgcaaaaa gttcttcttg ttgtaaacta gcgatgggat gtaatgcaca aagcgaataa
    11941 ttttctttta tcacatactg ataccattca ggcaattgat caggcgtgtt ttctgtcaag
    12001 ccgatataaa aatttctagt aaaaacattt tcaagtaaaa actgccaatg accgaaaaag
    12061 ttatcctctt tattgaaagc ctcagaaaca gaatgatttg ccggaacaac aaacgctaga
    12121 tggtcggttt ctgtcatttc tgcaagttcc aatttttctt ggatcatttt tcggctggaa
    12181 agtttcatca gctgctgata acctatttca tcttttgcgt acgcaagcaa gccataattt
    12241 ttttcttcat aagtataact aagatgtaat ccaatgattg gctgtactcc ttcttctaca
    12301 caaatacgat agaattcagg tgcaccgtat aagacatctt tatccgttat ggctaatgaa
    12361 gtgtagcctt gtttttttgc ttcttgtaca tattctcgaa tacgtatcgt actttgtaaa
    12421 agagaataag aagtaattgt ataaagttgt gggaaaaaca tcttgcattc cgcctttttt
    12481 ttcgttaaaa ttcactagcg ttaaaaggtt tgatcctttt actttcatta agatagcagg
    12541 cgctgaagtt gattattcca cttcatttgc ttgcttgtaa tctgtaggaa tgagttcttt
    12601 cgcaagaact caaattttat tgagatttgg tctcgtttct ttacattcaa agaaaatctg
    12661 gtaaaattaa gatagaaaag aggtgttctt tatgcgatat atcgttacat tattctgggc
    12721 tgtcgttcta ggtcaagtag tcggttatat tggtgctgca ttaacaagcg gaacatatga
    12781 ttttactttg acaacaatca tttcattcat tgcgggtgtc attatccttt tgattggtgc
    12841 agttgcgcca agaaaagaaa cctctgccca ttcgtaaaaa aacagccaag agcttgagat
    12901 ttctcaagct cttttttatt ttctactcct caccaccatt ataatctgct gcgaagaact
    12961 ttacgaaagt tgaataaaga aagaagaata tttgacagaa caaacaaact tgtcacaata
    13021 aaaacagcac tgtaattata agcatgcgcg actgtcgatc ctagcatggg acctaaaact
    13081 tgtccaaagt tgctaaacat ctgattgtag ctatagattc ggctgactcc ttcttgaggc
    13141 gtaatcttac tgatcagtgt attgacagaa ggcatcaaag cgccagttga aaagcctaaa
    13201 ataaagcgaa gtatacctaa ttggagcggt gtagtgacga atgccatagg taaataacag
    13261 caaaatgata aaatgagtcc tccaattaga actttttgat tcccaatctt atcccctagt
    13321 tttcctagtt tcggtgaaga aaagacagcg gaaacaccag ctatcgaaac aatcaagccg
    13381 ctgacaaaaa ggatattttc tgtacttccg ctcaattcac gaatatataa ggtcaaaata
    13441 gggctgatcg tagtgattcc tatctgtaaa atcaaggtcg taatgaagag accgatcaaa
    13501 atagagagat gatccatttt tgaaaaaatt tctttggtgc ttatcagatc ttttttctcg
    13561 attggatgga aatcttcttt gactaagaaa atggtcaata ccgttgtgat catcaacaaa
    13621 gcccctgtga tcaagaatac attttccata ccaaaccact gagccagtgc accgcccatt
    13681 gaaggtccga tcagatttcc agcaatcgct ccggtagaca atgttcctaa cgcccatccg
    13741 ctcttttctc taggtgcctg tgaagcaatc atcgctgtgg catttggaat atatccagac
    13801 aaaatcccat tccaaaaccg catgatcaaa agccaatacg cgtttggtac aaaagccaaa
    13861 gatcccattg ttaatgtcat ccctgctgct gcacggatca tcatgagttt tcggcctttt
    13921 cgatcagcta gatttcccca gattggtgca accacggcag atgcaaaagc agtcacagag
    13981 attgctagtc ccgaaaacaa ttcgatttga tcttttggtg ttcctagttg ctcaacataa
    14041 actggtatga atggcataac taaactaata cttgcaccag tgaaaaaaca gccaatccac
    14101 gcaatcacta aattttttct ccaattaatc tccatctatc atccctcttt cgtctctttt
    14161 atcataccta ttttcgaata aacaagcaac gatctcgcca gtaaaaagag aaaaatcccg
    14221 caaaggtcat tttatatgta acgttctttt tatgtaaaac aaaaagagat cagaacaaat
    14281 actgttccaa tcccttctct ttagttaaat tgttgctaga atgatgaagt tgataatgaa
    14341 taaaatatca acgatccaca aagcaacaga aacttgattg tattttcctt tgattacttt
    14401 cacaattgta tagaaaataa atcctgctgc aatcccatag gaaatgctat agcacaatcc
    14461 cataaagatc gatgcaaaga aggccggtaa agcttcttcc atatctagcc atttgatgtc
    14521 tgcaaaagaa gccatcatca ttactccaac taaaatcaat gctggcgcag ttgcttgatt
    14581 tggtacaatg gcgatcagtg gagagaataa actacttaat gcgaatagca ctgccactac
    14641 tactgaagtc aaaccagtac gtccaccggc cccgatccca gctgcagatt cgacataagt
    14701 tgttgtattc gatgtaccaa agatagcccc cacagaagta gcgatcgcat cggcaaatag
    14761 tgctttatcc atttttgttt tgaacccacg accatcttcc aacgcgatct cgtcttcttt
    14821 tgagaaaata cctgtgcggc gtcctgtacc gataaatgtt ccgatcgtat cgaaagtatc
    14881 agataaactg aatgcgatga tcgtcatcaa tacttgagga atcttggaag aatcactgaa
    14941 taaagattgc atgccttcag aaccaaatgc tgcaccgaaa gttgttccta attctttaaa
    15001 tgaattacct aacgaattcg tttgccaatc aatggaactt aaatcaacaa cacccataaa
    15061 aataccaagg actgtggtcg ctacgatacc gataagaata gccccacgga cattttttac
    15121 aactaaaatg gctgtcagta ccaaaccaat cacagccaat aagataggag cgttgtcaaa
    15181 gttggctaac gcagggacga ttccaccatt tatcgtaaca tttgtcgctt ttccgccttc
    15241 aacgacagag ctcgaaatag ctgactggtc tgctgaaaaa gataagaatc ctgcattttt
    15301 gattccgaca tacgcaacga aaataccgat cccgccacca atcgcatgtt gaagactttc
    15361 gggaatcgcc cgaataatca tttttcgaat atttgtgaca gtaataaaaa tattgattaa
    15421 tccgcaaata aacaccattg ccaaggcttg ttgccaagaa taaccaagcc caaaaacgac
    15481 cgtaaaagtg aaaaatgcgt tcaatcccat accaggtgcc tgagcatatg gtacatttgc
    15541 gaacaatccc attaccagcg taccaataat cgatgcaatg attgtcgcta gaaatactgc
    15601 ttggaagggc atacctgatg ctgacaaaat cgtcggattt acgaataaga tataactcat
    15661 ggcaaagaat gtcgttactc ccgccattat ttcagttgat acatttgtat tgtttttctt
    15721 taaaccgaaa aacttttcca taaaaagttc cactccctga atttttttct gatggaaaac
    15781 tataataata aaaaataagt aaaatagggc aaatccatcg aacaatttca aattatatag
    15841 acgtttcatt cgcttttcaa cgttttttta acaaaaaaag cgaaaataat acttaacgtt
    15901 cgtgtttagc ttatgtttag ctatttatat agccataact gtagtttttg ttaggaatag
    15961 ctgattgctc ttgttaatta cctaaaatat gctagactaa aacaaatgct agttgaggaa
    16021 ggaaggaccc ttattgaata aaaaattaat tgccatcgat ctggacggaa ctaccctcaa
    16081 tcaagattcg atgattacat ctaaaacaaa agagactcta aaaaaagcaa tcaatgccgg
    16141 tcatcatgtc agtattgcta caggacgccc attccgaatg agccatcagt tttaccaaca
    16201 attagaatta acaacaccga tggttaattt taacggcgca ttagtacaca tccctaatca
    16261 gcactgggat ggtgaaagag aaacgttgat caatcgtgaa atcgcttttg aaatcttatc
    16321 gcaaaaaaag cagctaaatt tagattttat cgctgctgaa aatcgtgata ctttttttat
    16381 tgacagtttt gatttctttg atgaaaaaat ttttgcttct tctcgtccag gagagaagaa
    16441 tttgctttct cctaaaaatt tgacgacaaa tcctacatct ttgcttgtcc gaacagacaa
    16501 acgttttgca ggagctgtat ctgctgaatt aacacgtcaa tttggcagtt atgtcgatgt
    16561 tcggacatgg ggtggaccta ctgccatctt agagattgtt tcaaaaggaa tccaaaaagc
    16621 aaaaggcgtg caagagattg ctaactacct gtctatcgat caaaaagacg tcattgcctt
    16681 tggtgacgag cataatgatt tggaattgtt ggattatgca ggttggggag tagctatggc
    16741 taacggtact gaccaactca aaggcattgc aaatgatgtc actcctttga gcaatcagga
    16801 agacggttta gctgtttatc tggaaaaact acttaaacta taatagagaa ttagattcca
    16861 caaaaaagag gttgtgacaa tttt
//
LOCUS       DACDIH010000057        16878 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDIH010000057 DACDIH010000000
VERSION     DACDIH010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.057"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..587)
                     /locus_tag="IV281_001990"
     CDS             complement(<1..587)
                     /locus_tag="IV281_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780972.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVF"
     gene            complement(735..1277)
                     /locus_tag="IV281_001991"
     CDS             complement(735..1277)
                     /locus_tag="IV281_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP8780973.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            1412..1801
                     /locus_tag="IV281_001992"
     CDS             1412..1801
                     /locus_tag="IV281_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780974.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1805..2569
                     /locus_tag="IV281_001993"
     CDS             1805..2569
                     /locus_tag="IV281_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8780975.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2698..3645
                     /locus_tag="IV281_001994"
     CDS             2698..3645
                     /locus_tag="IV281_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8780976.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3775..4515
                     /locus_tag="IV281_001995"
     CDS             3775..4515
                     /locus_tag="IV281_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780977.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4806..5474)
                     /locus_tag="IV281_001996"
     CDS             complement(4806..5474)
                     /locus_tag="IV281_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8780978.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            5608..5895
                     /locus_tag="IV281_001997"
     CDS             5608..5895
                     /locus_tag="IV281_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780979.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            6311..7849
                     /locus_tag="IV281_001998"
     CDS             6311..7849
                     /locus_tag="IV281_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP8780980.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            7846..8811
                     /locus_tag="IV281_001999"
     CDS             7846..8811
                     /locus_tag="IV281_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8780981.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            8798..10120
                     /locus_tag="IV281_002000"
     CDS             8798..10120
                     /locus_tag="IV281_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8780982.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      10183..10287
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10361..11104
                     /locus_tag="IV281_002001"
     CDS             10361..11104
                     /locus_tag="IV281_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8780983.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            11119..12111
                     /locus_tag="IV281_002002"
     CDS             11119..12111
                     /locus_tag="IV281_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8780984.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            12123..12860
                     /locus_tag="IV281_002003"
     CDS             12123..12860
                     /locus_tag="IV281_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8780985.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            12850..13572
                     /locus_tag="IV281_002004"
     CDS             12850..13572
                     /locus_tag="IV281_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8780986.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(13656..14279)
                     /locus_tag="IV281_002005"
     CDS             complement(13656..14279)
                     /locus_tag="IV281_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8780987.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQP
                     VVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYI
                     GRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            14674..15365
                     /locus_tag="IV281_002006"
                     /pseudo
     CDS             14674..15365
                     /locus_tag="IV281_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
     gene            15573..15863
                     /locus_tag="IV281_002007"
     CDS             15573..15863
                     /locus_tag="IV281_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8780988.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT         5765 a         2785 c         3433 g         4895 t
ORIGIN      
        1 ataaatacac ctagactgac gatgaagagc atatccaaca gatttcctgc gacttggatc
       61 gtcgaagggt tggtcagtgg cattaaagca aggacaaaaa aggaaaacaa gctgtaaata
      121 aaacaggtga caggaactaa tcgaaagaag agcggacatt tcaattggct aaaaatgagt
      181 gaaaaaataa aataagtaaa aatggataaa atcacgacta aaccattcat gataaagaag
      241 aaaagcgtat tgtgattaaa cgaataaaaa agaattgcta aaaaagaaaa cgttttaaat
      301 gcaatcaata cgcttgaagc ttcgtttaac gtaccaggat gtcgatgaaa aaaataggca
      361 ccgaaataaa cagaaaacag taagccgcaa agagaagaca aaatcaaaaa aaacggattt
      421 tcttcggctt ctgtgatatc agaatgatcc aaaagcaacc agcctaatgc actagaaggc
      481 gcatgtgagg tcttaaggta tgaataacat agaaaaaata agatcgtcaa agttgcaaaa
      541 aaagagccta aatgtaaaaa attagtggtt ctacgttgaa tttccataat atctccaaat
      601 taaattgata agtatataaa gtataataac aaattgatga gtatatgtcg atgtttttta
      661 atcaaaataa taaataaaaa ctgtctaaca tttaaaattt aatttagata ttagacagtt
      721 tttaagtttg tgagttattt tccttgtcgt tttctcactt ttttagtcca gaaaccacct
      781 gaaatatatt ttggctcata agaaatgata aaagcacgct cttcgatttc tttgatcagg
      841 cgatacagtt cacgttcgct ttttctagga gaaagtattt cgagaactag ccgctcacct
      901 tccagcccca ttgcagaagt ctgtgtcaca ccataaccgt gttctctcag tatacccggc
      961 aaacttttat cttctgcgct atttgctggc aaaatcgttg tgaccatgat gtatcctaat
     1021 gccaatttgt cttcaatcaa aattccgaca ctgataccta ctgcgtaacc taacgcatag
     1081 acaaataaat tcagtggatt atctaaacga tctaaaacaa gggataagcc gacgatataa
     1141 atcgtgatct cgaccatact gacaagtgga gcaatcagtc gatagccttt cattgtcaac
     1201 atgaaacgta acgtgttcag agtgatataa gcaaaattaa tgaggaagat catccccaac
     1261 atttttggat caatcatttg taaaactcct ttcatttata taacaacaat atcaaaaaaa
     1321 atcgtaagtg aagaaaaatt gaaactttat acatacaaaa aaatgataaa atgattataa
     1381 tagaattata aagataggtt aggtgattaa aatgaaaacg gtcaatcgtg tgttttgtgc
     1441 cagccacgtg ctaagtctcc ttctcagtat atacttaggt gtctattcga ttcaacagcc
     1501 aattgaggta gagaacattg tttttcagat gtcgctgaca gatggtttaa ttttaagcgt
     1561 ttttttcttt ttgatactgt ttattggtaa tattttcggt agcgttgcca gttttttgaa
     1621 tttttctatt tatccactcc tttcgctagc attaggagtg gtgggacttt gttctctgtg
     1681 cttatttccc gagccattca gtcctgcatt tatattattt ggtacactga acctgttcca
     1741 gacaactgtt ggagcatggt tgctatggcg atcaggaaat ttaatgaaaa taggtgagta
     1801 acaattgaat ccaattatat tgaaacgttc tgtccatcat attccagtgt tagaagttgt
     1861 tctgaaagaa ttaagagagg aacgtattcc cgttgtcatc tattatcacg gatggcagac
     1921 aagtaaggag ttagttttga cgcaaggaag aaaaattgcg cagcagggca ttcgtgtgat
     1981 cctgccagat gcgatgaatc atggggagcg caaacagccg gtttcttcta ttccatccta
     2041 tacattttgg gatagtatct acggaaatct gtttgaattt gacacactta tcaagcattt
     2101 agaaaaaaga ggtcttctac atgatcagct tgcagttggc ggggtttcga tggggggaat
     2161 gacaacatgc gccttgttgg ctaaacatcc tcatatagta gcgggcgtgt gtttgatggg
     2221 ttcccctgca cctttggaat atggaaaaga aatcgtaagg cgcgcatcgg aatataatta
     2281 tgtcgtacca aaagactatt ttgatttgat ttcatgggtt aagagctatg atttgtcatt
     2341 gcagccggaa aagttagctg gacgtcctct gttcttttgg catggacaag ctgacgaaaa
     2401 aatcccttat atacaaacgg aacgatttgt agaaaaaaat cggcataagt cttacggaaa
     2461 agaaattgtt tttcgttcct caaaaacaca gcgacatatg gtacaaatac cactaatgga
     2521 agaagcttcc acttttttag caaaaaattt gataaaaaag tataaataat tgaaacggaa
     2581 aatcactcat gacgatagag gattttccgt tttttatttt gggctataat aaactctgtt
     2641 aggaaaattt ggtataattg atagtaagaa tgacaacatg ttagaaagga agagagcatg
     2701 cccactttag cagatgttgc aaaacgtgcc aatgtttcaa aaatgacggt ctctcgagtc
     2761 atcaatcatc cggaacaggt gactgacgaa ttgaaagagt tagttttctc agcaatggct
     2821 gaactggatt accgtccaaa tattgctgca aaagcacttg tcacgaatcg gtcgcagatc
     2881 atcaaattat ttatactgga agaaatcgac acaactgagc cctattacat gaatttgctg
     2941 atgggaatcg ctaaacggat cggtaaagcg cattattctc tccaattagt tacaaatgat
     3001 gccttcgacg tcggttcttg tgatggatac atcatcacag gtgtcagaaa aaaagacttc
     3061 gattggatcg accgtttgga aaaaccagtt gtcttgtttg gggaaaattc tgaaggatat
     3121 gattatgtag acagcgataa tgaatatggt acagcaaaag caacgcaata tgccttagaa
     3181 agagattatc aaacgattat ttatatcggt atcgaagtgg acgagccttt tgaaatttca
     3241 agggaaactg gttatttatc tgtgatgaag gaagttgatc ttcctagtaa aatcttgcgg
     3301 tttccaaatc attcgacaga agtagaaaga tatatgacag aaaactgggc agattttaca
     3361 gataaaactt gttttgtatg tagttcagat cggctagcac taggagcagt aagagggatt
     3421 ataaatgctg gaggacactt gcccgaagaa tttggtgtga ttggatttga tggggtattt
     3481 ttagaccaaa tcagttcacc taaattgaca acgatcaagc aagatattat acgtatgggt
     3541 gaagtatgtg gagaaatgtt gttaaaaaaa atagaagaaa aaggggcgag tcaaggttat
     3601 cgtcgttttt ttcctgaact agttatcaga gagacaacga attaagtctt attactatat
     3661 atatatttat gaaattcatc gctattttct taaaataatc agcgcattgc tactgaaaac
     3721 aatgaatctg tgctattatt gagaaaagtt ccatttttga gaaggatggt ttgaatgaaa
     3781 gaaatcagac gaaaacattt ccgttttcct gcgtacgatg atgagttagg cgtaaaatta
     3841 accaacgaca agaagcgagt attatttgac ggacaagaag atcttttgac tgaacagaat
     3901 cagtccccgt ttgaacagat gaaagaattt catttcacga acacgaacac cgagaaaatc
     3961 cgtcgcaagc gtcgtgcaac aaatggattg aagtcagctc cgcgtaagga aaatttggaa
     4021 aagcataaag gaaaattacc ggattatacc ggccattacc aatcagaaat cacatcgaca
     4081 ggacgaaaaa aactttttgg tgattcctct cgttctgtca gacaaagtga aacaaggaca
     4141 gagtccaagc atactacacg aaacgcatct gttgacacga aggtgcggga gcgttcttat
     4201 tttgttccta aatatgtacc cgcatcgatt atcccagacc ctagagaaag caaaatttct
     4261 gaggatcatt taatcgaatc gatgaaaaaa acggatgact cttatctgtt gttcgataca
     4321 gaaccgacgc cgtatcaaga aaaaagaaat ggtgagccgt ctgtacgaac attcaatcga
     4381 acaaaaacgt tagaggaaac aaagcagtca gaagaaaaaa aatcgaaaaa aagccatggt
     4441 atcttagaac gctcattaca aggattgatc gatgatcaag gaaactcttt gggagaaaat
     4501 agctatttta aataagtgat atacaaattt atattcggct acagttggaa aggaaacgct
     4561 gcggcgtttc cttattttat actctatccg aaagtttata tcgtatcctc aaattttttt
     4621 gagtttaagc gaattttttc tcccaataag taagaaaata atggcaaaat aaaaaaacaa
     4681 actattttcg cagatcgttt gcaaaaatag tttatttttt tataaagaag attttactga
     4741 ataatatcat tttagtttac ataatgatat ttctgtaaat taaataaagt agtgaaaaat
     4801 aagaatcagt gctctaactg ttcaagagga tcaagtggtt caaatgtttc tgtagtctgt
     4861 tttgcttcca attggggcaa aacagatcca atttccagat acttcacttt attcaatgta
     4921 taagatagaa tagaacagct aagtaatgta tacagaaaat gtgtgagatc caaataaagc
     4981 aaggagacaa ctaaaatacc aaaagtaatc aagaaaagac ttttgccaat cttaatatga
     5041 aaatattttt caatggcttg ataaatgata tctgttccgc ctgtgcttcc gccgaatcga
     5101 aagacgagac cgcaaccaat gccagaaaga atacctgtca tcaaactatt gatcagtaga
     5161 ctatctacag caaaatgtat atgaaggctc tcccataaat gtaaaaagaa aactagcgca
     5221 aatgttccga cgatcgtccg gagaaaagcg tgttttccaa aaagaaacaa tgagataagc
     5281 aataaaggta aattaaaaag cagtgaagtg acagaaggag cgaatccaaa aagattatgc
     5341 aaaagcaaag caataccagt aaccccacca tctgccagct tagatggaat attgattcct
     5401 gtcacagcta atccgtagat tcctgcgcct aaaatgattc cgcacaattc tgtaaataaa
     5461 tttaattttt tcatttctag cctcttttct tttgaaaatt ttcagcaact tctttattat
     5521 actgacttat actcttactt caagaggaaa atgaaaaaaa attatttttg gctgtttctt
     5581 tttatttatc aaataaggag tggaaaaatg cgagcagcag acttattgac aattcttaca
     5641 aaatcaccca aaaaaacaga gtatcaagga gtttttttgg ctcaagaaca aaaactggtg
     5701 cctgtgacaa tggtccagat aaataaagca aatcaattta tctttttatt tgaacacggc
     5761 aaagcaccac ttgctatgaa agacatcttt ctttttttga tgaagaaccg tgagaaagct
     5821 gtttattatc agtcaaaaga agaactcatt cctctttatg gagtgaaaga gatagaaaat
     5881 aaagtggtga tataagtaaa aataagaaaa caagacgttt atgatgtcaa aacaaccatt
     5941 ttttacattt ttggcaaaaa agtgtcatga tacagtataa tattcgttga acaggccaat
     6001 aaaggtgttc tcccccacga gatttctact ttattggctg tttcttttta ttaaaataaa
     6061 agttgtgtgt gtaaaaaaga tatagctgat tccaagtgac aatttcgtat agatttttat
     6121 ggtattcgta gtgaaaaaac gagaaccaaa aagttgatcc gactttgttg cttggattct
     6181 ttttttgtca gcatatttag aggtattttt ttcttttttt tgttcgtgct gtaaggttaa
     6241 ggcaagacaa gttagttggg aaaaaggaaa gaaatgttta tacttaaaga ataacagaaa
     6301 ggaaggtcgc atgaataatt ttatacaaac attaagtgaa agaaaaggcg agttgctgga
     6361 agctgcattt caacatctgt ccatttctct catttctttg ctgattgcag cactgattgc
     6421 tattccttta gcgatttggg cagccaatca taaaaaaatg gcagaagtct tattgcagat
     6481 cacgggtgtg ctccaaacga ttccttcact tgccttatta ggtttactta tcccatttgt
     6541 agggatcgga actgttccag cattgattgc acttgttatt tacgctttgc ttccgatttt
     6601 ccaaaacacg tatattggtc tagctgaaat tgacccgtct attgaagaag cagcggtagc
     6661 atttggtatg tccagaatga gacgcttgct gaaagtagag cttcccatcg cattacctgt
     6721 cattatctca ggtatccgaa ctgccttagt tttaatcatc ggtacggcta ctttggcagc
     6781 attgatcgga gcaggtggat tagggacatt tattttgtta ggtattgacc gaaatacacc
     6841 tgttttgaca ttgattggtg cgatcagttc tgccttgctt gcaattatat tcagcggact
     6901 gattcgctgg ttgcagcata agaaacctcg atattccatc attactttag cagcagtggt
     6961 gtgtttattt ggcggcgttt ctttatatca gtctgccttg tttaaaaatg aaaccatcac
     7021 gattgctgga aaattaggag cggaacctga aatcctgatc caaatgtata aagaattgat
     7081 tgaagacaac acaaaaacag aagttgaatt aaaaccgaat tttggcaaga cgagtttttt
     7141 gttcaatgca ctggatcaag gtcaaatcga tatctatcct gagttcacag gtactgtcct
     7201 agaaagcttg gtgaaggtac ctgaagcaat gaaaagtgct gatttgacaa aagaaaaaac
     7261 atatgacgaa gcagaagaat tattgaataa gcaattcgat atgacattat taaaaccaat
     7321 ggcttaccaa aatacgtatg ccttggcagt caagagatct tttgcgaacg aacatcaact
     7381 taaaaagatt tccgatttgg cagctatctc tgatcagatc aaagcgggat tcacactgga
     7441 atttattgat cgttcagatg gatataaagg aatccaagaa gcatatggct tgaatttttc
     7501 aagtgtacaa agcatggagc caagtttgcg ttaccaagca atcaataatg gagatgtcaa
     7561 tattgtggat gcctattcca cagatagcca gctcaaggag tacgatctag tgactctaga
     7621 agatgatcga gggttattcc cagcttatca aggcgcaccg ctgatgaaaa aagattttgc
     7681 taaagaacat cctgaaatcg ttaaagcact gaataaatta agcggaaaaa taacagagga
     7741 tcaaatgatt gatatgaatt accaagtcaa tgtcgaaaag aaaaagccga cagaagtagc
     7801 acgaatcttc ctgaaaaatg aaggattagt aaaggaggaa gatcagtgac taaattaatc
     7861 gagttcaaac atgtgcaaaa aaagtatgat ggaaagtatg tcatcgatga cctaaatctg
     7921 gcaatcaaaa aaggagaaat ctttgttttg gttggtcctt ccggtagtgg aaaaacaact
     7981 actttgaaaa tgatcaatgg attatctaaa ccatcagcag gagacatcta cttcaaagga
     8041 aaatcgttaa atgaatataa tctgcagaaa atgcggtgga atatgggata tgtgctgcag
     8101 caaatcgctt tgttcccgac catgacagtc aagcaaaata ttgaagtcat ccctgaaatg
     8161 ttaggttggg aaaagcagaa aagagcagac cgtgtagatg aacttcttca aaaagttggc
     8221 ttatctcctg atatttatcg agatcgtatg cctagagagt tgtctggagg agaacagcaa
     8281 cggatcggga tcatccgagc tattgctgca agcccagatg tgattttgat ggacgagcca
     8341 tttagcgcat tagatccgat ttcaagaaac tcattacaag aattggtttt gtccttacac
     8401 gaagaattgg gcacgaccat cgttttcgtt acgcacaata tggaagaagc aatcaagcta
     8461 ggtgatcgta ttgcatttat gaaggatgga gaaattatcc aatgtgacac accagagcag
     8521 ttattgatga atcctaaaaa cgactacgtt cggcatttct ttgatgaacc gaaacagacg
     8581 aaagagtggc gagtagaaga tttagtcgtc aatggatatt tcttaaatga aataccagaa
     8641 aatgctcgtc aacaagtttg ctttgatacg cctatgaaag aagtatattc gcttctttcg
     8701 gtgtatccaa gcattgtgat agtagaaaag caaagaagta ttgggtcgct gacaagtaaa
     8761 gaaatctttg cttttttaag tcgagaggag gaaggaaatg aaaacatatg atgcaatcat
     8821 catcggttca ggtgtgagcg gcttaagtgc tgcttatgga ttgaaagaag caggtaaaac
     8881 agtactagtg gtcgaagaag atctatgggg cgggacttgt cctaaccgtg gatgtgatcc
     8941 taaaaaagtc ttgttaagtg cagtagaggc acgtaatcgg gtaaaacaat tgagtggaaa
     9001 aggttttaac gagatcccaa ctgccaactg ggaagaactt caaaaattca aacgaacatt
     9061 tacggatcca gtcccagaaa gccgaaaaaa acaattagca gaagctgaaa tcgatcatct
     9121 ttcaggaacc gctcgttttt tggatgattc atctattgaa gtcaacgaag aagtcttcca
     9181 tgcggattac ttagttttgg caaccgggca aagacctacg atcttaccag tggagggaaa
     9241 ggaatacttg aaaactagtg cagacttttt atcattgcct gtactgccta aagaaatcat
     9301 ttttatcggt ggtggttata ttgcttttga gctagctacg attgccaatg ctgcagggag
     9361 caaagtaacc attgtccatc ataatcaacg gccattaaaa gaatttgagg cgtctttggt
     9421 agaggaagcc gtccatcaaa tggaagcatc tggcattcag tttgcttttg gtgttgaaac
     9481 ccaaaagatc atttcagaag gtactcgcta tcgtttagtc ggaaaagaga cggaattagt
     9541 agcagatatg attttttgtg cgactgggag acagccgaat acagaatctt tagcacttga
     9601 gcaagcgaat atcgtctttg ataaacacgg aattgcagtc aatgattatc tgcaaacgag
     9661 caatccaaaa atttttgcgt gtggagatat tgtttcaagg aaaaccccaa aattgacacc
     9721 agtcgccact tttgaaggaa attatgtagc taagcggata acggatgcaa caagcgaacc
     9781 tatcaaatat ccgattatac ctacgatcgt ctacgccagt cctaaacttg ctgaagtagg
     9841 agttacaaaa agtcacgcct cttcgtcaga tcaagtggta gagatggatc ttaccagttg
     9901 gttcacctat catcgtgtca atgaaccagt tgctaaggct gaattgacct ttgaccagca
     9961 aaattatttg attggtgctg cagtgattag cgagcaagca gacgaattga tcgatgattt
    10021 gacactcgtc atcaatcaaa aattgactaa aaaagagttg gatagctata tcatggggta
    10081 tccgacattg gcaagtgatc tctcctattt attgaaataa tcagcatgat aactaaagcg
    10141 cttgttgctt tttccttctt gtgctacact ttaaaaaatg aatacctgtt tctggggatg
    10201 tcgtagacga catgagagta agttgtgaac ttagacccat tgaacctgtc agttaatact
    10261 ggtgaaggga gaaacatagc agacgcttga agcgggttac tatgtcgtgc ccatttcaag
    10321 cgttttttgt tattcatatt agaagaaagg aggtaacaaa atgaagaaaa atctttatcc
    10381 agcagtcagt atggcattca ttttaattat ttggcagatt gttgtcatag tttggaaaat
    10441 acctgccttt attttacccg gacctgctgc tgtattttct gcattagcgg cagatgcaca
    10501 agtattactt tcccattctg tcacgacatt aaatgaagca gtgcttggtt tggtaatagc
    10561 tagcgttctt tcttttatta ctgccttggc aatggattat tggaagtttt tagaaatgtc
    10621 tttttatccg ttactagtga tttcacaaat gttgccaatc atggtgttag ggccattact
    10681 taccctatgg tttggtttcg gtatgcttcc aaaagtgatc cttgttgtct tgataagcta
    10741 ttttccgatc gtagtttctt tttcggatac attgaggaaa gtatcaaaag agcaaatcat
    10801 ctttttgaaa acgatggggg cagatacagg gaagatttat cgaatatata aaataccagc
    10861 gggtttatcc ggcttctttt ccggtttaaa agtggctgct acttattgtg tcagcggagc
    10921 agtcgttgga gaatggctaa gcgctcaagc aggcttaggg tattacatga tacgcgtaaa
    10981 aaacagctac cagatcgata aagtttttgc tgcgatcatt tgtgtcattc tattgagttt
    11041 attgctgaat ggcgcttgtt cattattgaa aaaagggtat tatcgtatgc tttatatttt
    11101 ttaagagaag gggatcaaat gaaaaaatta tggcttttat taccgctact gttgctttta
    11161 tccgcttgtg gcactgcaaa agaaacaagt agcaaacaag aaataaagga tttgaaaaaa
    11221 gtgacacttg ttttggatta tgtaccgaat actaatcata ctggcatcta cctagcaaaa
    11281 gaaaaaggct attataaaga ggctggttta aatgtccaaa tcatcgaacc aggagataat
    11341 tcgacaagta ttggtctagt aggtgcggac aaagcacaat ttggtgtaag ttatcaagaa
    11401 gatgtcactt atgctcacgc agatggacag aatataccag tcaaagcaat tgctacagtg
    11461 atcaaacata atacttctgg attcgccacc ctatcagaca gtaacatcca ttcaccaaaa
    11521 gattttgaag gaaaaacata tgcaggttgg caaagtccaa gtgaagaagc tgtcttaaaa
    11581 gctgtgatgg aaaaagacgg cggggacttt tcaaaactga caatggtagg aagcaatgga
    11641 gaagggccag aaagcttagg aaaatcctca gatatccaat ggtactttga aggttgggat
    11701 atgatcaaag caaaagaagc gggtattgaa ctaaactata tcccattgaa agaattagat
    11761 gaacggttag attactatac tccggtaatt attacgaatg atcaactgat caaaagtgat
    11821 ccagaattgg tacagtcttt tatggatgcc acgaaaaaag gctatcagga agctattaag
    11881 gatccaaatg acagtgctaa actgttgcaa aaatatgcaa aagaaaatga tcggacgttt
    11941 ttagaagaat cacaagcatt cttgtcaaaa aattatacgg atgatcctga gaattgggga
    12001 ttgatggaag aaaaagtttg gcgcaattat acagctttta tgcaggaaaa cggattgatc
    12061 aagcaagatg taccttcgca agaactattc actaatcagt ttataaaata ggagtgatag
    12121 atatgtcaat ccaaatcagc cacttgaatg tagtcattga aaacctgaaa atattagaag
    12181 acatccattt cactatccct aaagatacct ttacatcgat tgtcgcgcca agcggagccg
    12241 gtaagtcaac attgctgaaa actttaacag gagtccagcc tatgacttct ggaacgatca
    12301 aagtagacga tcaaaaagta actggtctcc atacagcatt tagttatatg ccgcaagaag
    12361 atatgttact tccttggctg agcgtctatc aaaacgttac gttatatcaa aaaatcaatc
    12421 atctaacaat caatgaagaa caagtaaggg attatctgga tatctttggg ttaaacggct
    12481 atgaacattt tttgccggaa caattatctg gcgggatgaa gcagcgaacg gccttgttac
    12541 gaacaatcat gaatccttct tcttacttgc ttcttgatga accatttgga gcgttagatg
    12601 ctatgaccag agggcagatg caagactgga tgttgaagct gcctaaaaaa gcaaagcgga
    12661 ctactttact tgtgacgcat gatatcgaag aagcaattta tttatcagac aggattcttg
    12721 tgctttccgc ccgtccggca catgtgattg ctgagattca agtgcctgaa aaacgacggt
    12781 cacgtgaatg gctactgcag caatcagagt tgaagcaaac gatttatcag ttattggcag
    12841 gtgaattaga tgttaagtga tgctcattgt catttagatg ggagtcagag tttagcactc
    12901 cttcagcagg aacacacgat tttgacgatc atcaattgtg attcgccaga agaatggaag
    12961 gaaaacagac agcttgcagc aagtaagact caagccttaa gttacggtat ccatccttgg
    13021 aaagcagata gctatacgtt tgaacaagtt gagccttttt tgaaaaaagc aaggatcatc
    13081 ggtgaaatag ggttagataa tatttggacg aatgtaccga tgactacaca aaaaaaagta
    13141 tttgagcgac agcttgcttt tgcagccata aatgagaaac ctgttgtact gcacacaaaa
    13201 ggatgcgaaa aagagatctt aaattatatc caagcttacc ctaatcacta tttgatccat
    13261 tggtattctt ctttggacta tcaacaagac tatattgatt taggctgtta tttcagcatt
    13321 ggtcttgatt tgaaaaagaa tccagcagtc tggcaactag ctaaagcagt cccaatcgac
    13381 cacttactta ttgaaacgga tggactacat gcagcagagt gggctttagg ggggattttt
    13441 tcagaaaaag attatgtctc actgcttgaa gaacagttaa agacaatcgc tgagatcaaa
    13501 gagctatcag ttgaagaagt taaaaaaaat acttatcaaa acttggaacg tttcattaga
    13561 ttaggtaaat aaaaaagaga tgtgacacaa ctagtgtcgc acctcttttt ggctttcttt
    13621 gtataaaagt aaactgtttg attaataaat gaatcttagt accaaccgtt tgcttcccag
    13681 aatgctttcg ccgcttccca agaaccataa cgtgaagtaa catattgttc tgctactctt
    13741 tcttggtttg cagcagagta gtcaccattt aagtaagaag aatctaattg gtaacgtccg
    13801 atgtaacgtc cgttcgttgc tgtgtaagaa ccgcttgatt ctttttgtgc gatccattct
    13861 tttgcagagc ttgtgcttgc tggagtagca gcagtttctg ttacagcagg agcaggttgt
    13921 gtttcagcaa caggcgctgc ttgtttttcc gtttgaaccg tttcttgtac aacaggttgt
    13981 tcagcttgga ctggtgcttc ttgaacggct gcttgaggtt cagcagctgc ttgtgcagag
    14041 ccttctgttg ggattgttaa ttgttgtcct gagtaaatca ggttaatatc agaaattgaa
    14101 tttgattctg caatggcatt gatcaaggag ttatcaccaa cgtatttttg agaaatcgtt
    14161 gatagagtat cacctgattg aacggtataa gcttcgtccg catgagcagt cgttcccatg
    14221 aagaatgcag cgccggcagc caaagttgtt ccaaaaagta aagttttaag tgatgtcatg
    14281 aaaaatctcc tttattcgtt aaaaagtttt atttgttgtg ttcaacgagg aatactttaa
    14341 cacgtgaaaa tatttcatag gtaaaagaag catgacaatt tatgacaaat ccgcgttttt
    14401 tgtaataaat acagtaatct atgaaatgaa aagggttacg tttaataaaa cttgcttttt
    14461 tgtttttgtt caccataaat atttatttaa gaaagtatta acctgcattt ttctctataa
    14521 aaaagatttt ttaaatagat aaaaaaggag gattaaatga gtatttgata gtatttatat
    14581 taaaattaca tatgtaattt aaaaacgctt gacattgtgt ttgagatcac ttacttttag
    14641 catgtaaaag tattggatta aggagctatg caaatgaaaa agaaatttat tactggtggg
    14701 attgtcgttg catttctatg tctaattggc tacacaggca cgcggtacgc aaatgaaatt
    14761 attttgtggg gtggagagac agatattgat acaatcaatg agaatctgac acagcttgat
    14821 catacattga cagataaaga gcaagcgatt acagatctta ccgctcgact agcacaaaaa
    14881 gaacagacat tgcaagattc aaaggcagat gtccaagaat accaaacaaa agtaaatgaa
    14941 ttagaaaacc aaaaaaatca atcggcagca gaaaaacaaa acttagaaag tcaattagtc
    15001 agcaaggatg cacaactaca aagcaaacaa aatgaaatca atgtcaaact agatgagatc
    15061 aaccaaaaaa atgatgaaat caatcatctg aatgcagatt ggacagcaag actacaagaa
    15121 gcacaagcaa aactttcaga attacaaaat actgtaaatg gtttgaatca acaaatcgca
    15181 acactgacac aagaaaaaac aaatgtagaa atcagttaac agatacacaa aagaaattag
    15241 acgaagcaac tgctgaaaac tcaagcttga aacaatatat tcaaaagtta gaaaaagcaa
    15301 aagatgaagt ggaagataca gctgataaat ctcagcaaat tgttgaagaa catttgcgta
    15361 aataatcttt cttaacaagt aaacctaaat tagtaaagaa gcaacagtaa tctatactgc
    15421 tgcctctttt ttttagtaca tgtccttcat cttcgcagga agtgccaata tgattttaag
    15481 aaaggtaata gacaaaaagt caaaaagata aaacagagtt tcttctttac aaagggaatg
    15541 tatgttcgta taatggtatt gaggtgaagg ttatggagaa aaaatatgga acagtagata
    15601 agatccgtac tttgcaagtt agtgccagac cgttgatccg tttcagttta aatggggtca
    15661 attgtttgat tgcttcccat agtttgaact tcctagctga agtagacgaa gggatgaagt
    15721 tagtcgttac aggttattat aataatagaa agcaatttgt tgtgagatcc tatcatgtgt
    15781 tagggaaacc caaaattgtg gtagaatatg agaaaagtct atacccaaga aaaaaggtcc
    15841 gacaagatga aagaataaac tagaaaacaa gcggctgaga caaaaatgtc ctagccgctt
    15901 gttttggaaa gatggatgca gacgatggga tttgaaccca cacgagcata cgctcacaag
    15961 aacctcaatc tatatataac tacatgtatt taagtatttg tgatgaatca ctaaactttc
    16021 caaactcatt tgagggatat tgcttaaaga ttgaacagat caattcgaag ccctattagt
    16081 attcgctcaa tggggggatc agaggccata aaaagagact agtcattact agtctcaggt
    16141 agtggaattg taggatttta aacgttcctg gagagtaccg gtatgtaact cgaagaggtg
    16201 attatcatag tcgtaaaagt aaattgaaga agcctcgcct ttcacacgag aacgtcctgg
    16261 ttcgacagtt aaacctaaca tttggatttt tgagacaaac gaatctatat cttgttcgtc
    16321 aattttaaaa gcaatatgat tgtatgtctt tggcagttta ttcgacgaat tctgcattac
    16381 tgcaatccat aggtccttaa ctaagaaaaa tttttctgga tataatgaaa atttttttgt
    16441 tgactatcgt agatttcttt tgcatcaaat aaggtattta acaattaagt atttaaaaga
    16501 taacttttaa gttatcttta agtaatgctt aaagctatgt tatactctat taggaaatat
    16561 aagaaggagt ataaaaatga acaaacataa tagaaaaata acgaataaca aagaactgat
    16621 cttgttacta atagggattt ggggtatctt tttactcttt aatatgtata tgccgactat
    16681 gagagcggat gacgtagttt atgcaagtag attagatgaa cttggctatt tgggtgcttc
    16741 gatagaacat tataagacat ggagttcaag aattattata gaactattct taatgttctt
    16801 ttctaaacac tttatgctat ggaaattatt aaattctacg attatgttag gaattgtagt
    16861 attactttgc aagtatgt
//
LOCUS       DACDIH010000058        16856 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDIH010000058 DACDIH010000000
VERSION     DACDIH010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.058"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..328)
                     /locus_tag="IV281_002008"
     CDS             complement(26..328)
                     /locus_tag="IV281_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8780989.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="IV281_002009"
     CDS             complement(353..2446)
                     /locus_tag="IV281_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8780990.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="IV281_002010"
     CDS             complement(2935..3699)
                     /locus_tag="IV281_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP8780991.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IV281_002011"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IV281_002011"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP8780992.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="IV281_002012"
     CDS             complement(4704..5780)
                     /locus_tag="IV281_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP8780993.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="IV281_002013"
     CDS             complement(5804..6397)
                     /locus_tag="IV281_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP8780994.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IV281_002014"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IV281_002014"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP8780995.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="IV281_002015"
     CDS             complement(8067..8366)
                     /locus_tag="IV281_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP8780996.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="IV281_002016"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="IV281_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP8780997.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="IV281_002017"
     CDS             complement(10121..11032)
                     /locus_tag="IV281_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8780998.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="IV281_002018"
     CDS             11152..12009
                     /locus_tag="IV281_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8780999.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12196..12558)
                     /locus_tag="IV281_002019"
     CDS             complement(12196..12558)
                     /locus_tag="IV281_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781000.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            12904..14343
                     /locus_tag="IV281_002020"
     CDS             12904..14343
                     /locus_tag="IV281_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8781001.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14350..>16856)
                     /locus_tag="IV281_002021"
     CDS             complement(14350..>16856)
                     /locus_tag="IV281_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP8781002.1"
                     /translation="TSTEGLSQEERQKRLTDQGYNEIKARQQTKRWKKIAKHFTDLLM
                     IVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEESLNGLKQLMGQEAIVL
                     SNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVEESILTGESEASEKSHA
                     VLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKINQALQSVEQQTTPLIK
                     KINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIALVVSMVPEGLPAVLTMI
                     LSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNEMTVVDVAAKEEACVLS
                     IMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKIEAKIPFSSEYKYMATM
                     HAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARKGQRVLGFAYKKVDSKQ
                     ELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKMITGDHKDTAKAIAEQI
                     GLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLRIVKALQKNGEIVGMTG
                     DGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTIAKAVKEGRRILDNLQK
                     TINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTITLSYALGFESPDPEIM
                     KRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQSLQQTILLQNIVLAQAV
                     YMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPVGQQLIGTVSLTMTQQL
                     LIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
BASE COUNT         4763 a         3590 c         2913 g         5590 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattactta ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    12301 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    12361 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    12421 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    12481 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    12541 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    12601 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    12661 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    12721 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    12781 ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa
    12841 tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga
    12901 ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct
    12961 ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata
    13021 gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa
    13081 ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta
    13141 acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat
    13201 ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat
    13261 gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag
    13321 atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt
    13381 tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc
    13441 catgaatccc attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa
    13501 acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat
    13561 tgccgattag attcttctat ccataccaac agcacactct cctccctgta tgactctgca
    13621 ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct
    13681 tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc
    13741 tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca
    13801 gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata
    13861 ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt
    13921 ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac
    13981 gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta
    14041 ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc
    14101 caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa
    14161 cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca
    14221 gatatcttga tttccaattt ccctctagat tctctagata tccctattgt atacatttct
    14281 tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta
    14341 tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt
    14401 catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa
    14461 taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa
    14521 aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg
    14581 caaaagacct tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc
    14641 ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg
    14701 catagccaat aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa
    14761 atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg
    14821 atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca
    14881 taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat
    14941 caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa
    15001 aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac
    15061 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc
    15121 ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    15181 atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac
    15241 atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc
    15301 ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc
    15361 tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc
    15421 tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg
    15481 ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa
    15541 aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc
    15601 agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc
    15661 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt
    15721 tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc
    15781 tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca
    15841 gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat
    15901 ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    15961 ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac
    16021 ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc
    16081 aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac
    16141 taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt
    16201 tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat
    16261 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    16321 tcctgaaaaa cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg
    16381 acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg
    16441 tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc
    16501 tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc
    16561 ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca
    16621 gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt caatatattc
    16681 actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    16741 atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc
    16801 ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgttt
//
LOCUS       DACDIH010000059        15953 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDIH010000059 DACDIH010000000
VERSION     DACDIH010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.059"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..440)
                     /locus_tag="IV281_002022"
     CDS             complement(<1..440)
                     /locus_tag="IV281_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8781003.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYEL"
     gene            663..1454
                     /locus_tag="IV281_002023"
     CDS             663..1454
                     /locus_tag="IV281_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8781004.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1420..2373
                     /locus_tag="IV281_002024"
     CDS             1420..2373
                     /locus_tag="IV281_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HAP8781005.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2771..3745)
                     /locus_tag="IV281_002025"
     CDS             complement(2771..3745)
                     /locus_tag="IV281_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8781006.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3860..4294
                     /locus_tag="IV281_002026"
     CDS             3860..4294
                     /locus_tag="IV281_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8781007.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4419..5405)
                     /locus_tag="IV281_002027"
     CDS             complement(4419..5405)
                     /locus_tag="IV281_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAP8781008.1"
                     /translation="MEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGAFFRVHHFAPQIG
                     SPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQGRVQLGVGRGSPE
                     QVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPMFPQGSGKLRIEP
                     YSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQQANQLRAYKEAW
                     KQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYHRNPTIFGKTFAG
                     EPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIAPELGWR"
     gene            5833..6255
                     /gene="arsC"
                     /locus_tag="IV281_002028"
     CDS             5833..6255
                     /gene="arsC"
                     /locus_tag="IV281_002028"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HAP8781009.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRAIKIMAEDGIDISKQTSDLIDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDL
                     KDPAQAVGSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
     gene            complement(6535..8457)
                     /locus_tag="IV281_002029"
     CDS             complement(6535..8457)
                     /locus_tag="IV281_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP8781010.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(8544..10484)
                     /locus_tag="IV281_002030"
     CDS             complement(8544..10484)
                     /locus_tag="IV281_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8781011.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            complement(10772..11512)
                     /locus_tag="IV281_002031"
     CDS             complement(10772..11512)
                     /locus_tag="IV281_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HAP8781012.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            complement(11525..12616)
                     /locus_tag="IV281_002032"
     CDS             complement(11525..12616)
                     /locus_tag="IV281_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HAP8781013.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            complement(12606..13712)
                     /locus_tag="IV281_002033"
     CDS             complement(12606..13712)
                     /locus_tag="IV281_002033"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HAP8781014.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            complement(13731..14408)
                     /locus_tag="IV281_002034"
     CDS             complement(13731..14408)
                     /locus_tag="IV281_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HAP8781015.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            complement(14419..15198)
                     /locus_tag="IV281_002035"
     CDS             complement(14419..15198)
                     /locus_tag="IV281_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HAP8781016.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            complement(15227..15577)
                     /locus_tag="IV281_002036"
     CDS             complement(15227..15577)
                     /locus_tag="IV281_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HAP8781017.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            complement(15593..15949)
                     /locus_tag="IV281_002037"
     CDS             complement(15593..15949)
                     /locus_tag="IV281_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781018.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
BASE COUNT         4733 a         3344 c         2689 g         5187 t
ORIGIN      
        1 agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa tgtttcatcc
       61 atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc tttgattcct
      121 aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata aaaataaatt
      181 gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca ttccgaatgt
      241 ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct ttctcttgca
      301 atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg ttgtgcagcg
      361 attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa actaggaacg
      421 aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt taatttattg
      481 ttctactaag ttcatctttg ttttacaaga acattatatt tctgttagtt tgttttgtca
      541 acgcttcaaa agaaatttat tttgttaaat ttgatctact tgaaaataaa atcgtttatc
      601 tatataatta agaagaacaa agtcgaacaa aaattgactt ttatgttaaa ggagtaattc
      661 atatgctgaa aaaagaaaga caagaacaaa tcattacatt attagagaaa gatttttatt
      721 taactaattc agaaatcgct aacaaattag gcgtatcaga aatgaccata cgccgagatg
      781 tcactgaatt agcaaaaaaa aataaagtaa aaaagctata tggtggcgtt gaacgaaagg
      841 aaccttataa tcgagaacta tcgactcaag aaaagattga tacgaatgta gccgaaaaaa
      901 aatatattgg tcaagtcatg aacagtatga ttcccgataa ttctactatt tatgtcggtg
      961 ctggaacaac gatcctatat gcacttcctt tcattcaaaa gaaaaatcta tttgtaatta
     1021 caaacagttt gattgctttc aactatttaa agaatcatac aagttatcgg attttactta
     1081 ctggtggcga attttctcct attacagaag agttcattgg ggaagttgca gaaagatctt
     1141 ttgaaagttt gaatgtcgat atctctttcg ctgcaacaaa cggcatattt gataacaatg
     1201 tgacaaccgc tcaatttatt gagggaaata ttcagaacgc agcgctcaat gcagcaaaaa
     1261 tcaaatgcat tgttgcagat tattcaaaat taaataaatc agacgtctat acttttagaa
     1321 aattaaccga ttttgattat ctaataaccg ataatcattt atcagaacag gatttcaatc
     1381 attattcaaa atacttaaca atattaaagg agcccaaaaa tgatactgac aatcacgatg
     1441 aacccatcca ttgattccat ttattttaca aaacactttg aattaggtac gatgacacgc
     1501 tttgattctc cggtacgcta tgtaggcggc aagggaatca attgcggacg agcttctgct
     1561 ctactaggtt catcagttat tttaacaggg tttttgggcg gttattttgg cagtatgatt
     1621 caagatatgc tgaaaaatga aaatttgttt gatttgcatt ttttacctat tgcagaagaa
     1681 tcaagaggtg ccgtcacgat catgcatgat gatgaaacgc agacagagct agtggaagct
     1741 ggtcctgaaa tagcagatac tactatcaaa aaattaatga atgatatcaa actaatcagt
     1801 aaaaataatt acctttcagt cattacaata aatggttctg ttaacaatcc agatcccttt
     1861 ttgtatgcta aattattaca atatatcaaa gaacaaatag atccttccac ctatatatta
     1921 atggatatat ctggacaaca tctccgtgaa gtcttagcaa gtaagtataa accgatgttc
     1981 atcaaaccta atatccatga gttttcagat atcatccatc aaaaaattac tcaaaaaagc
     2041 gacataaaac accatctaaa aacaaatcca ctatttaaag atatcgatgt tatcatgatt
     2101 tcttgcggct cgtatggtgc agtagtcaaa gtaaaagaag aattttggaa tgttgagatc
     2161 cctaagattc ctatcattaa tacaacaggc tctggggatg ccactgttgg aggttttgct
     2221 cattcattag aacagaatta tcctttaaaa gaatgtgtaa ggtacgcaat ggcttgcggc
     2281 atgtcgaatg cacagcaatc agaagtcggg aaagttattc cagctgatgt aaaaaattta
     2341 gcaaaaagca ttaaaatcac agaaatgaaa taaatagtac aaagtaaata attgtttata
     2401 tgaaatgaga cacaaagaga ctgtgacaaa aaacgtgtgt accgaccccc aaaagttaga
     2461 ttttttggtc taacttttgg gagtcgatac attgtcacag tccttttttc gaataaacgg
     2521 tgttctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctatt
     2581 ttttgaaatt gctccttgtg actcagagtt aaacggcagg ctcacgacct cttggtataa
     2641 acggtgttca aaagcttgca tctacgctaa tgagtccaac caagtcaaaa atgaggagct
     2701 tttttggctg tccttattac ttgatgcagg gcagtttttt cataatctct tttagattaa
     2761 atccttttgg ttacacaagg ttctcttcaa agaatgttgc cattttttca aatggaatga
     2821 tgtctgtctg gtcgtataga tctgtatggg atgctcctgg aatgatcatg agttccttgt
     2881 tgtcaccttt tagttctttg aaagcgtcct ccccaaagta acgtgaatga gctttttccc
     2941 cgtgaatgac taagactgca tgacggattt ctggaatata atgcaatagt tttgcattca
     3001 taagtgacaa ccctgatgtc ctattccaac catcattgga gtttaaagaa cggacatgat
     3061 atccgcggtc tgttttataa tacgcatgat agtctttgac aaattgagga gcatcttcag
     3121 gcaacggatc aaccacacct ccagcacgtg cgtagtcccc ttgtttgaaa tcaatgatgc
     3181 gttgtgcatt taattcttca cgcatttcat atcgggcatt ttcatccata acatcgaagt
     3241 agccatttgc tgttactcga gtcatgtcat acattgtgga aacaactgtt gctttgaccc
     3301 gcgtatcgat agctgctgca ttcaatgcca tacctcccca tccacaaata ccaatgattc
     3361 cgattctctc agggtttaca tttttttgta cagataaaaa gtctactgcc gcttggaaat
     3421 cttccgtatt gatatcaggc gaagcaacat atcgaggtac tcctccgctt tctcctgtaa
     3481 acgatggatc aaacgcaatc gttaaaaagc ctctttcagc catcgtctgc gcatatagac
     3541 cagacgattg ttccttcact gcaccaaatg gcccgctcac agcgattgcc ggcaatttct
     3601 tttcacttac ttcttttggt tcatataaat cagcgactaa tgtgatacca aaacgattcg
     3661 ggaagctcac ctttcgatga ttgacttgat tgctttttgg aaatactttg tcccattctt
     3721 gtgtgatgtt taattcttta ttcatattct agactctcct ttgatcaaaa ttgacatttg
     3781 tttttgaatg tattattatg ataaaactta aacttgactt taggtcaagg taaaaatttt
     3841 attttaggag gattttttta tgtattccat tggacaagtg tctgaaatgt ttggattgcc
     3901 tgtttctact ttacgttatt acgacaaaga gggattattt cctgatatcc agcgtgtttc
     3961 tggtattaga aaattttcag agaatgagtt agaggcttta agagtcattg actgtttgaa
     4021 aaaatctggt ttagaaataa aagacattaa gcaattcatg gaatggagca aagaaggcag
     4081 taagacattt gaggttcgta aagaactatt tgaaaaacaa aaagaagtag tagaaaaaga
     4141 aatagcaaaa ttagaaagag tactcgctat gctaaactac aaaagctggt actatgaaga
     4201 agcaataaaa gccggcaatg aagaagccgt attgacaatg attcctgatg atttacccca
     4261 acatgtaaaa gaagcttatg tacattctca ttgaacaaac ggataactca ctctccttct
     4321 ctatgaactt catgaaaaca tgaataaaca cctctaactg attgttagaa gtgtttattt
     4381 tttcactcaa gtatcctgca cttacaaaaa tatccttttt accgccatcc taattctgga
     4441 gcgatatctt ttacgattgt ttcaagcaag tgcatgttat attctacccc caaagtatta
     4501 ggaatcgtga caagtactgt gtctgcttct ttgattgctt catcttcagc tagctccttg
     4561 attaattgat caggttcacc agcaaaagtt tttccaaaaa tcgttgggtt tcggtgataa
     4621 gcaagatagc cgacagcatc ttgttgcgga ctaccgtctt gtccaaataa gcgtcgatcc
     4681 aaatcagtgg tgattggctg aatcgagcga gtaactaatg ttcttggttc aaaatcatga
     4741 cctgcttgct tccacgcttc tttatacgca cgtaattggt ttgcttgttg gatatggaat
     4801 ggctcgtggg attcgtaatt gactaatgta gatgattgca gattcattcc atgttgtgct
     4861 gcccagattg ccgtttgctg cgaaccagct ccccaccaga ttctttgtcg taatccttct
     4921 gaataaggtt ctatccgtaa ttttcctgat ccttgtggga acattggttg aggatttggt
     4981 tcttcaaatg gttttccttc aagtaattcc aagaattcaa gggtgcgttc ccgtgttacc
     5041 tctttgattt cttcttcact gtaatcataa ccaaagtatt tccagccatc tagaacttgt
     5101 tcaggtgaac cacgccccac tcctagttgc actcttcctt gcgtgatgat atctgtcaat
     5161 ccggcatttt ctgccatata ataagggttt tcatatcgca tatcaataag tcctgtaccg
     5221 atttcaatct tacttgtctt tgcgccgatt gccgctagca aaggaaacgg cgacccaatc
     5281 tgaggggcaa aatgatgtac gcggaaaaac gctccgtcca ctccgatttt ttctgcttcc
     5341 accgctaaat caatcgattg caaataagca tcttgagctg atttcacaag cgatccttgc
     5401 tccatccaat gtccaaagct taaaaagccg atttttttca aggtaatgac ctcctttaaa
     5461 cctacaaaaa tggaaggttt tttattttta atatgaactg attctactac tttttttctt
     5521 aaaagacgaa taattgttct cgcctcacaa tcaagaaatg tatcggctta ctacacccct
     5581 actttcttca tcatctgccg tgtcttaatc tcttttaaca gtctgttaca tcgttgattt
     5641 ttgactcaaa atgattgttt ctaacatgta tttatggtaa aaaagacaag attagttctt
     5701 tgtacacatc gatttatacg ctgaacaaaa agaaatatat aaactaagaa acccattgtt
     5761 ttttcgctcc atcacataga gtttacttta gataaacagt gacatttact aatccataac
     5821 ggaggaaatc aaatgaaaaa aatctatttc ttatgtacgg gcaattcatg ccgcagtcaa
     5881 atagcagaag gctacgcgcg taaatacttg tctccttcaa tgtttgagat aagaagtgcg
     5941 gggatcgaga cacatggctt gaatccaaga gctataaaaa tcatggcaga agacggcatc
     6001 gatatttcca aacagacgtc tgatttgatt gatagagatt actttactca ggcagacttg
     6061 atcattacac tttgtggtga tgccaaagac aagtgccctg ccgttccaaa caatcacgaa
     6121 caccttcact gggacctaaa agatcctgca caagctgtcg ggagcgaaga agaaatatta
     6181 acggaattca gaaaagtcag agaaggaatc aaaagaaaag ttttagactt aaatcaacgt
     6241 acatcgatcg tataaagaag tattcaaaat gatttttcaa gcactaagaa acaatgttaa
     6301 aaaaatagct ttccatattt tacaaaattg tgccttattg ccgacaaatt gtctcttgaa
     6361 caccgtttac cagtagtcaa gacatcgtaa gcaactataa aaaattataa gaaactcaaa
     6421 ataaaaggat gtgccacaac tttggccaca tcctcttttt ttgtatttag ttttctactt
     6481 ccaaataaat agatagaatt attataaata taaaggcagt tttttaatcg ctacttattc
     6541 ataactagat aacaacgtta agatttctag cggatgattg gattgcatca actgagccat
     6601 ttgttctggc tgcttttcta gatagtgaat aacagaaaat atttttttga actctgcatg
     6661 tttcaaatca tttttcttta ttgctaatag cagtacgata cgtatctgtt ccccgtgcca
     6721 taggactggt tctttcattg tagcaataga aatcatcgaa tgcttcacat acagcggatt
     6781 accgtgaggg attgttacaa atggtgctac tctagtactg gagtgactgt ctctttccaa
     6841 gacagaatcg atgaattccg gctccacata atgatgttcc actaagacgt ttcccataaa
     6901 gtttaaaata tcggctgggt ctgtccgttc aagttgagga aagatcagaa atggatgagt
     6961 aaagcttgct agatcaaatg tttttttctt cttgataggc acatgctcca gcatatattt
     7021 tgtcaaatag gctagatcag tttctgtgat cattggtgag atctcgatta ctggaatgct
     7081 tatatgttta aatggtaacg tagccaagat aaaatcaaca gagggaaaat cttgtctctt
     7141 taattctggc ttatattcaa ctagatccat aatcgttaat tctggaagtt ctctgttcag
     7201 ccgtgcttcg agaaaagtag ccatagcttt tgaatattca ctaactaaaa ccgtttgata
     7261 tcttctactt ttctgatgtt tgtgtcgctc gatcgctgct tggaaatgga tcgttaaata
     7321 agctgtctcc tcttgtggaa cataaaaatt ttccttttca aaataatctt ctaaaattaa
     7381 ctgtaaaact aagaaaagtt gtgtgtacgt actaaacaca tttttcgtca atggattaga
     7441 aatctgcaaa ccataatgga gtcgaaaata agttgaagat aaatgagacc ttaaattttg
     7501 ttttagtagc tgatcttctt ttaatgaata gcctagaaac tgttccactt gttcgatcaa
     7561 atgatcagca agtgcgctag attccttttg aaaacgtgtg gctgcttgtg aatttgtaaa
     7621 aagattggcg atacgcaaag ccagataatt gatttcatca ggagaaaatt caattggata
     7681 gatggtggct agttttgcta atatttgact acttaacttt tgtgcttggg tgtgttccac
     7741 gacttcgttt tcgtctttac ttaatttcac tttctggtac tgtttcattc gctcgagcat
     7801 aaagtagatg tgaatctcca cactagccag tccattcggc tgttctaaat ggttttcata
     7861 aaaaacatct tgtaatgtat gttggataac ttttaatgta tctttagcga agaattcttt
     7921 caacgacgga ctttgtggat cttctttctg catatttcgt aatgtcttaa caagcaactg
     7981 tcttttttct tgttcatcgc ctttaagaaa agttcctagt tttgttttgg atactagctg
     8041 taaatggtga tactcaaaaa agctttgcaa ctctgtcaat tctttacgaa tcgtacgttt
     8101 tccaacatat agtttttctg aaagttcttc aagactaaat ccgtcttttg ctactagcag
     8161 atggaaaatc aataactgac gaagctgttt tttatccatt tctgtttgag tctcttcatt
     8221 aagcgctcgt ttcagttgtt ccctttcgtg aggcagacca ttcaacaaga tacctgtacc
     8281 tttttttctt tcaaacatta aaggcagatg tttgtctctc aaccaagttt caatggtatt
     8341 caaatcgcta tgaatcgttc tttctgacac atggaaatgc tctgctagct tttttgttgt
     8401 tatataatta ttgttctcac ttaattgata gatcaaagat tgctgacgct gattcataat
     8461 gtgcagaaac tcccttctaa ataaaaaatc gatcatgata acacatctat tatgtctcat
     8521 cacggtcgat ttttcatctt atgttacatt tcgcttaata attggtgaac tgttgtttta
     8581 tctggcgctt ccatcagtgc ttgacggaag tcatcatcca tcaatttacg tgatagacgt
     8641 tgcagtattt tcaagtgcat atcaccttgg ctgttttcag gtacagcaat cataaagatc
     8701 atatgtgcag gttttccatc taaagaatgc caatctaccc cagattgact tctggcaaat
     8761 gccactgttg gtttaagaac ataagctgat tttccatgtg gaatagcaat ccccataccg
     8821 attcccgttg tactttcatg ttctctatca tagatgactt ctttaaatct ggttttatct
     8881 gtgatcgccg ctgttttttc taaacgttgg actaaatctt cgattactcc gtcttttgtt
     8941 gttgctgttt cacctaaaac gattgcttca tctgacataa tatccgtgat agaatcaaaa
     9001 gtttcagttt tcgtataaac atcacttgtt tcagtctctg atagatctgc tactgtcaca
     9061 ccatttgctg cagcaaccgc agtttgttgt aatgtcggtt taaggattct taacattagc
     9121 acggttacag caataccagc aatcactgct acaaaaaaca tggctacgtg atccactgct
     9181 cctaaaaccg ctacgatcgg accgccatga gctacatgat ctgtcactcc tgaaagcatc
     9241 gcgatcacac ttcctgtcat tgccccgatt acaatgcttg gaatcacccg tagtggatct
     9301 gttgaagcaa acggaatagc gccttctgta atcccaaaca gtcccattgc taaggacgcc
     9361 ttaccagctt ctcgttctgt tttcgtaaat ttggttttta aaatcaatga tgccagcccc
     9421 aatgctaatg gagggataca gatagctaca gcaattggcc ccataatgga ataattgcct
     9481 tctgcaatca agccagatcc gaacaaaaag gctactttat tgaacggccc ccccatatca
     9541 aaagcaatca tcgctcctaa gatcatagct agaacaattt cacttcctcc ttgcatccct
     9601 gctaaaaagt gagtcaatga actaaataga agagctactg gttctccaat aacatagata
     9661 aacacaaaac caactgccaa tgatcctaaa attgggataa caataattgg catgatcgat
     9721 tgtaaagctt tcggtactcg cactttcttg attcctaaag caatgaatcc tgctaaaaat
     9781 ccagtgatga tcccgcctaa gaatcccgca ttcgcatcac taccatataa tgctccgttt
     9841 gccgctaaca tcccaccaac aaatccagga acaagtcctg gacgatctgc aatactttgc
     9901 gcaataaatg cagatagaat tggaaccatc aaccccatag cgatacttcc gatactattc
     9961 atcgttgccc aaatcgttcc ttctggtatc ttgatccctt caggtgtagc tgttcctcct
    10021 aatgttaaag aaatcgcgat caataaccct cctgtaacta cgaaaggtac catgtacgaa
    10081 acgccattca tcaaactctt atagattttt ttacctaaat tttctgattc acttttattt
    10141 tcggaagaaa taattgcctc cgtactttta tagactggcg cttttcctgc taaaatatct
    10201 ctcaccaact catctgcatg gtggattcct tcttgcacac ctacggttat caaaggttta
    10261 ccgccgaatc gtgatttatc tattttagta tctgctgcga tgattacacc agctgcttct
    10321 tcgatatctc ttgcagtcaa ttcattttca ataccgatgg aaccatgtgt ttcgactttt
    10381 acctcggcgc caactgcttt ccccgctttc tctaagtttt ctgctgccat gtatgtatgg
    10441 gcaataccaa ctggacagga tgtgattgca ataatttttt tcattttcct accgcctttc
    10501 tacatttata gtatataaaa acggtttctt tttattaagc acaaaatgat tccgtaataa
    10561 ctgaaattct tggaataaat agaaatttcg ctaaaaaaat tgatatacga tttattatgt
    10621 tgaataaaat aatattggaa aatcttacat ttctgtactt ctgcttacgt acatctaaat
    10681 tattatctac attaacaatt tccgtaatta attataaaaa gcttattcca tttaatttca
    10741 ttctggaata agcttcttat tttctaatga gttagtgaat aatttttttt accatgctta
    10801 atagtatctt tacgtctcct atacgcgtat ctcctgtttc tttatctata attgaactat
    10861 aaacatgagg aatcactttt tttacacctg catcgattgc aattttcaga atttcttcaa
    10921 agtttgaaag atcaattcct cctgttggct ctaaatagaa ttcatatttt gcgcaagctt
    10981 ccgcaactgc tttaaattct tccctatgtg ctaagccttt catcgggaaa tacttgattg
    11041 aagtacctcc catgtcttgc agcatttgaa tggcagtttc aataggcact tcagctgctg
    11101 gcgcttgtga acttaacgga cctgtagcaa tattgacata gccgatctta cctgttggtg
    11161 aaaccaatcc attgatgact gtatcatttt ggcccaataa agcacgagaa gtccccacgc
    11221 cagtaaatac ttgattgaca tgttgtggtt gtaaaacccc agataaacgc gaaaccatct
    11281 gactttggtt tggatctcca gcgcctaatc caactgacaa cgcattattg gttgcgtctt
    11341 ggtacctttt catatcttcg attgctgctt catcgctagc ataatttttc gatagcacac
    11401 ctagtacgac atgcccttcc gctgcttcat agcatgcttt tgcattttca actgaatgag
    11461 ctaatacatt taaacataca cgatttttat aataatttgg gataactgac aatttaaaag
    11521 cctcctacat gatttctctc aatcttttta taattttttc catttcgttt tggctgacgg
    11581 aacgaatgtc gaactcaatg attccgttat ttgcttgata ttctcgggtg tagactgctg
    11641 gatttcgtgc ttttaattcc tgaatgactt ctttcgcact tttatcgcca ttaatcgtta
    11701 cacttgctcg ataaatatct ctgccggcac catcttgaac tattttcgct gaaaggtcag
    11761 gaagttgatt gattgcgtta acaaaaggcg ctaatcgttg cttcatggag tcaccggatt
    11821 cgctcccatt ttttaaataa tcttctagtg cctgggtaaa gcctaaaata ttatctttac
    11881 cgattttcat tgctcggcca atacctttag actgtagccg aatccattga atatacttct
    11941 tttttcctat gaccagtccg gcactcggac cttcgattgc ttttgcccca gagtaaatca
    12001 ctaagtcaac gcccatttcg ctatatttga ataaatcttc ttccgcagct gcatcaacga
    12061 tcaatggcaa acgatgagct ttggcaactt ctaccatttc tgccaccgtc agcatacttt
    12121 tctgaactgc atgatgactt ttaatatata gaagcgcagc agttttttcc gtaatcatca
    12181 tggaaacatg ttccggtgta cacatatttg catagccagc ttctatgact tttcctccac
    12241 cttgagcaac cattacttca accggtgtcc cataatctac attatgtccc ttaggtatga
    12301 tgatttcccg tctagcgatt ttttctgtat agggatgata gacatggtac atgtcgccct
    12361 gtccgattaa agcagcaaca gattgagcaa tccctgctga agcagaagaa acgatttgcg
    12421 catcttccac tttcagtaat tgagaaagat aagcgccggt tttgatggca agatcactca
    12481 tctcaaaaaa atgctcgcct ccaaatcgtt gtgctgctag gacactttca gataccttag
    12541 aaacccctag gatcgtcatt tttcccgatg cattaatgac ttcttttaaa tcaaactttc
    12601 catagctaat tgtcatatat gacgcctcca ataattgttt taatcggtaa aatttgttct
    12661 ttagctatac gtgtaaatcc gttggaatct ttcaattcct tttcattttc aacaatctca
    12721 aatatcgtca aatctgcatc ataattgaca gctaactggc ccttattctt caaatgaaaa
    12781 ttccgtgcag gaactgctgt aactttttca atgatttctt cccaagaata gccgactacc
    12841 ctcaattttt ctaaagtagt tgctagatca aaaactgggc catttgtacg attgcggatg
    12901 tagatatccg tactaataga ggtagctttc attcctgctt ttagtgcggt ttctgccaca
    12961 tggaaattaa agctatccgt cccatgtcca atatcaaaga cgacaccatt attgtaagca
    13021 tcccagacaa agtttttaat ttgatctgtc tttggatcta aaataccgtt tggttttcca
    13081 ttaaaacaat gggtcaatac atcgcctctt tccattcttg acaatacttc ttctaactca
    13141 ggaggtgcag aaccgatatg aaccatcaat ggcagatcat tgttttctgt ctggattttt
    13201 ttagctaatt ctaatggctt aatgccattt tcaccaacaa ctgtcttact cattctcgct
    13261 ttaatcccga caataaattc cggcagttct gctaacgcag tacttactag atcttcttga
    13321 atctttgtta gatcagcaag ttcatcttgt ttgacaatgc cccatttaga gatattcatt
    13381 agcgcataca catttgtctt gaccgatttc gtcaattgat aaaattcacg gatgttttct
    13441 gctcccgtag ttccagcatc aatgaccgtc gtcacacctt tttcgacccc tatctgatca
    13501 ggatagtcat aataaagatt catcttttca tagcagtgga catgatcatc gatccaccca
    13561 gcggataaat aatactttcc ctccaagtca actacttctt gcgccggctg atttatttct
    13621 gctgcaactg ctttaatcat gcctttttct atagcgacct ctattttaga tccatcaact
    13681 aaccttccat ttttaatcaa agtatcaaac atttaatgac cctctttcta ttaatttact
    13741 gccgggaaga ttgctcctaa aatcattgct cctaaaatag cacctccagc aattggtttt
    13801 ttccaagcat agaatcctag tgctcctaat gtagcgccaa tgccgattgg aatagatgct
    13861 gcacacgcgc ttaatataat taatggaccc aaaaaacgtc cagatgaatt tcctgccccc
    13921 atcatgacgt cagcaccaaa agtggaattt gattgattga tcgtaaattt acgaatcaag
    13981 acaatgatgc caccaacaat caaacctaga accaaaccag tcaataaaga gagtgcaaaa
    14041 ttatcgattg gtgccgctat tttcatactt aagagaattg ctggtacccc aattccgatc
    14101 ccagttagga tcgaaccgcc taagtccaag atcccaacta aagagccttc taaaattctg
    14161 gcaaataaga aactagcacc aaacgctgcc gctgcgccat aggatccgcc atccagcccg
    14221 gcttttaaca tcgccacgat cgaaacttca ttgaatgcac ccacaccata catcacatac
    14281 atgtgtgttc ctgcaaatac agcagcggac ataattccta cgattagcgg aaaagaccag
    14341 tctgcaaacc aaaagctctt ttgccgcttt gcttctaata cttttgcttg ttcttctgtt
    14401 gtctccatga ttctcctcct atttcacact aaagatgttg tgcaagttat caagccaatt
    14461 aggcacttga agctggaagc tcttgatcat ttccatatca aatccacgga aaaatccact
    14521 gaaaataaat aataaaacaa tagcgatcat catgctcttc gtgacgcgat tccaaccgct
    14581 ctcatcgacg cctttaccaa tcaagattcc tagaacaaca ccaggtaccg catttcccat
    14641 aatcaattgc gcgataccac caaaaagagt gccccaaaaa ccagttcgtt tccctgcgtc
    14701 taaagctgcc aaccaaaaaa taactggcat tactgtattg atcagaatcg tcgctgctgg
    14761 taccaaaacg gaaaccgccg tcacttgtaa cgcctctgga attgctgcag ctgttgtgtt
    14821 taagaaggct accaccactg ccccgataac cgctccaatg atccccattt ttttcggatc
    14881 atgcatggtc tgggtgacat ctttgttttt tgctagaagt gctgccgctg cccaattggg
    14941 aatgattcga tgcgtcacat cttgtgtgaa tgcaccagca ccaactgaag aagcccatgc
    15001 attaaagaaa aagcctaaac caaatgaaaa atgagaagct ggatcaccca tgcaggcatt
    15061 catttcgcct aatgttctaa aagctcctaa accttgtgta ttaggtgcat gaaacattcg
    15121 agctgccccg acagatgctg caaaaccaag taatcccccg ataatgaccg atttgactag
    15181 aatgattacg aaatccacgt tattctttcc cccttatgat tcaaaattag atttttttag
    15241 ttacaaatgg aatggcaatc ccatttggat cttcagtcat ttgctggctg aacgatatgt
    15301 ctgccaattt tatttctgtt acagccacag taacagagag ttcgacttca tagctaatac
    15361 gttgccgtgg gaagaagaaa aatagaaagc gttctgtgta tttgttttcg accgccttta
    15421 aaatatccac attgactggt tcgattcgca ataatacgtt tgattgttcc attattttgt
    15481 tttgaatttc tgctaacgct gatgcaaaag catgttgctt tgtttcccct ttaccggaga
    15541 cgataacttt ttgctgaatt gtcttttgaa cgctcatttc atgtccctct ttctattttc
    15601 catgcttttt gataaattct tctgtcagac gtttccctaa ttcctcttga tccataaagc
    15661 cgaaacctag aacagttttg ccatccttga tagctgtgat cccttcttca atcgaacgca
    15721 taccatgtcg ttctggatag ccatattttg ttgctgctgt taatgcacct gcaccaccac
    15781 taccacaaaa agagataccc atatctgcat gctcttgttg catcacatcg cccaaacgca
    15841 tatctgcccc cattcctggt acgacaactg cttttccacc agctgcttct acccctttgg
    15901 ccacattttg tcctttaccc atacggtctg caataactac tttaatcatg att
//
LOCUS       DACDIH010000060        15857 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDIH010000060 DACDIH010000000
VERSION     DACDIH010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.060"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..741
                     /locus_tag="IV281_002038"
     CDS             <1..741
                     /locus_tag="IV281_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP8781019.1"
                     /translation="DIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEFP
                     FMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDYD
                     KKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKKQ
                     VLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGFG
                     QAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            734..1807
                     /locus_tag="IV281_002039"
     CDS             734..1807
                     /locus_tag="IV281_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781020.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            1955..2473
                     /locus_tag="IV281_002040"
     CDS             1955..2473
                     /locus_tag="IV281_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781021.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            2454..2846
                     /locus_tag="IV281_002041"
     CDS             2454..2846
                     /locus_tag="IV281_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP8781022.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            2922..3857
                     /locus_tag="IV281_002042"
     CDS             2922..3857
                     /locus_tag="IV281_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP8781023.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            3990..4769
                     /locus_tag="IV281_002043"
     CDS             3990..4769
                     /locus_tag="IV281_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP8781024.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            4762..5079
                     /locus_tag="IV281_002044"
     CDS             4762..5079
                     /locus_tag="IV281_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8781025.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            5323..6933
                     /locus_tag="IV281_002045"
     CDS             5323..6933
                     /locus_tag="IV281_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8781026.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            7090..7938
                     /locus_tag="IV281_002046"
     CDS             7090..7938
                     /locus_tag="IV281_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8781027.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            8169..8648
                     /locus_tag="IV281_002047"
     CDS             8169..8648
                     /locus_tag="IV281_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781028.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            8681..10909
                     /locus_tag="IV281_002048"
     CDS             8681..10909
                     /locus_tag="IV281_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8781029.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            11038..12681
                     /locus_tag="IV281_002049"
     CDS             11038..12681
                     /locus_tag="IV281_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8781030.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(12754..13299)
                     /locus_tag="IV281_002050"
     CDS             complement(12754..13299)
                     /locus_tag="IV281_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP8781031.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(13322..13807)
                     /locus_tag="IV281_002051"
     CDS             complement(13322..13807)
                     /locus_tag="IV281_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8781032.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(13797..14609)
                     /locus_tag="IV281_002052"
     CDS             complement(13797..14609)
                     /locus_tag="IV281_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP8781033.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(14748..>15857)
                     /locus_tag="IV281_002053"
     CDS             complement(14748..>15857)
                     /locus_tag="IV281_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP8781034.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         5193 a         2877 c         3200 g         4587 t
ORIGIN      
        1 gatatccctg agatctaccg tttatacaat ggccgattga atgaagaatt aaaaagaagc
       61 tcaaatgaaa atcacgagat cgatcaattg ctgatgcgta agatccatga tcgtttttta
      121 caggaatttc cttttatgga acaagtagaa aacatctcgc caacgatgag ctattacaaa
      181 atcggcactt attaccatga tcggcccacg gtcggtattg atgcaagtct gccagaaatt
      241 tttgttttgc ctaagatcga tgaagagtgg gagaaatatt cccaaccgga gtcaacggac
      301 tatgacaaaa aaatcaatga gctagacgca aaggtgatca ctgctcagac agaaattgaa
      361 cgtttagatg aacaaataaa agaaatcaat cgacaaatga ctgatttgaa cgacaataaa
      421 ggattcttga atcgtaaaaa aatcgaggaa gaaattcaag aattagagaa gaagaagcaa
      481 gtactatcta atgaaaaatt aggctggctt ccttatattg agacgcctga aacaatccag
      541 cagcaaaaag aagcattgaa gcaagaagca agagcagacc agcttcgtgc agccatcgta
      601 gaaaaagaac aaaggcaaat cagacgctat ttcggtagca aagagggatt cggtcaagca
      661 atccatgaat tcttgatgaa ttatcttggc aatgagaatc ctaataatca atctaatgaa
      721 ggaggttccg agtatgaatg aacacgatga agcagaatgg acaaaaaatg aagaacaaac
      781 agaagatcat gaagaaacga acacttcatt ggaagaacaa acaagtaatc ctctggatga
      841 cacagaagaa atccctatgc tagaggaaac aaaaatacac gaaaaagaaa aagtaactcc
      901 gacgaatacg aatactgcag aaaacaccca tgcttacgaa ataactgaaa atcccgggta
      961 tttagaagga aaagagcaag atcatcaaga tatcgtcagt ttgattgaaa cagcggaagc
     1021 agaattagcg gatttacgta tgagaaaaga tttgatcgaa tcagattttc cacaattatt
     1081 agaatcatat gatcgtttta tattgaatga agaaccaatc tccacgatcg gaaagacaag
     1141 cctgatcgaa tacttcacgt atgaattact gaaaccgttg aatgatattg gtctggaacg
     1201 gtcaaattca gcttatgaca catggaaaac aagacatttt tcgatcaatt atacgttgaa
     1261 tggacaaaac gaattagtct tttcctttgt attaccgacc atcgatcaac gataccaagt
     1321 ggaatcgatg agtctcctaa acgtatcacc agagacaatg gaaatcaatg tccaagatga
     1381 ccgggtgctt tctttgatcc gacactggtc agtggatcga gtattctctg cagggcagat
     1441 cacgatattc aatcacaaat tgaatcaatt attagcccat gcaagaacac ttggttttac
     1501 cgttaatcag acattactag acaatacgaa gccacttcgt ttgaatcttc agagcgaatt
     1561 tgaattgacg aatgaagtat tggatgatat ttttatcgtg gcaatgaaaa atccatccta
     1621 cgatatggaa aagatcaacg aaactactta tcaagtacga ttagataaag gacagtcttt
     1681 aacgataaca acaaacgaaa aagatcaaac agtcttgtcg atctcatctg gagactaccc
     1741 acgatctgtc attgatttct ttatcaatta cgaattcttg gtcccattaa tggtgagaaa
     1801 gtcctgattg gataagttgg attaccagat gtcattcaga taattctgtt ccaaaggact
     1861 tgcggcagtt atccagaata attgtcacaa gtcttttctg tatgtactaa atggacgtga
     1921 ataacttgct gatgaaggag ggatttcatt aggaatgtcc acaaataaaa tagcacagct
     1981 tagcttattg tctgctgctt gtgtagcagg taggatcgct tttcaattta ttccgaattt
     2041 ccagccaatg actgctattt ttttatttat agtactttac ttaagcttaa aagatgcgtt
     2101 aattgtcatg agtctttcta ttgcgatcag cagtttttat tttggtgttg gaccatgggt
     2161 catcggccaa tggatcagct atactgttgt tctgagtttg ttttcaatcg tctgcttgag
     2221 aaaaacagtg caaaggaatc tatggttaaa gggagtctgt ttttttctgg cgggcatggt
     2281 ttacggaata ggtatgaccg tttttgatac gctgttatat cgtttgcctc aaccttggat
     2341 ctattacctt caaggggtgt cgtttgattt gatgcacagt ataggcaatg ttgtgttctt
     2401 tcttgttttt ctgccagtcg tcaaacgttt ttacaatcat tcaggaggaa aaaatgaaaa
     2461 aaataattta tagtttgctt gcaatgagtg tcagtatact cattttgaca agttgcagta
     2521 caacctatac acaatcggct tccaaccaaa aggaggaagt aaaaacatcg caaaaagcaa
     2581 caatcacgtt acaagaaaat ggtcaaaact tttcggaaaa agaagtgttc ttcaaaaaag
     2641 gcgatgattt actgagtatt ttgaaacgaa attttgaagt caaagaagat aacggcttca
     2701 tcacttcgct tgaagggcac tcacaggatg agaaaaatca actctattgg atgtttactg
     2761 tggacgggaa aagtgctact actggtgcca aagagatcaa attgaatgat ggacaatcgg
     2821 tcattttcaa tttgtctaaa ctataagttt cataaaaaac tgttaaaggg cgaatattgc
     2881 cataattcag tgagttcagt tgatgaaaga aggaaaataa catggaaaat tttaaaaaaa
     2941 gcggaatgtt ttccgtaatt gctgctctag gggctaatat tctagttgct atcagcaaat
     3001 ttgtcggcta tgcaatcagt ggcagtgccg ccatgctaaa cgaaagcatc cacagtatcg
     3061 ttgattgcag caatcagatt ttgctgttat tcggtgataa acaagccaat aaaggacaga
     3121 gcgagttgca tcaatttggt gaaggacgag caaaatactt tttcagtaca atcgtcgcaa
     3181 tgatgctttt cttcggcggt ggggcacttg gggtgatgga agcttttgaa aagttgacgc
     3241 atccatccca tgaagtgggt aatccgatca ttgttatagc aatactgtta tttggtttgg
     3301 taatcgaagg aagttcgtta cgcgtggcaa tgaaagaaat caaagaattg aatactgaaa
     3361 aactttccac gatgcgcttt ttgcgcgaaa gtcgacacag tgaaatactg attattttta
     3421 cggaagactt gtgtgctgta atcggtttgg tcttggcttt agcaggaaca atattgactt
     3481 atgcaaccgg gattgctgca tttgatgcga tcagcggatt gctgatcggt ttcttgctga
     3541 tgggagccgc cattttccta gcaaaagaat tttatagtct gatcatcggt gaaagcgtga
     3601 cagcaagcga tttatcaaaa atcaagttag cttttgaacg tcctgagatc agtcggctga
     3661 tcgatataaa aacagttcat ttaagtccaa cggagatttt agttgcagca aaagcagata
     3721 tcgttggtaa caaagagaat gaagcttact cggtcatcaa tgatattgaa aaaaatatgc
     3781 gtcagtctct tcctgacaag aagatgtata tatacattga aacagataaa tatgacccaa
     3841 attattctcg taaataaaac gtttattcat gaattgaagc aaaaatgaga aaaatataat
     3901 cttgttttta aataaaacgg ctacaattta gcgaatttat gttacaatga gtccgctgtt
     3961 tatttgttgt aattaaagga gatattttaa tgaacaaaaa gaaaagtgcg ccatggcttc
     4021 aagctttgca tgcagtgatg ccactttgta taagctatat accagtagga ttagcttgcg
     4081 gagtattgtt gcaaaaagtt ggatttgatc ctctcttatc aggactgtta tcgattctgg
     4141 tcttttccgg tggcgcgcag tttttagttg catctatgct tacgacgcaa gcatcatttg
     4201 caacgacatt attgatggtt ttctttttag aattacgcta tacattatta ggagcaagtc
     4261 ttgccggttt tatgaaaaaa gaaaagcgga cgtttttggc tgtcttttct caatcactga
     4321 atgatgaaaa ctatgcggtg aactacttaa agttttcaac tgacccttcc tggaataaaa
     4381 gaaaggcttt atatgtcaat tggttctcaa tgactgcatg ggccggtagc aatatgatcg
     4441 gtaatttttt cggctcggtg attcgagtag atgctgacct tgtccatttt gctttgacgg
     4501 ccatgttcat tttcatgttt atcatgcaga tgaaaagcgc actactgatc tttacaggtt
     4561 tattctcagg cgtattaggg gtcgtattca tggtattatt ccaaaatact ttcggattga
     4621 ttgcagcaac agtcattgct tcatttgccg gatttatgct ggagaaggca ttcaaaaaag
     4681 aaaaagctaa gaaactaaga gaccgcggta aagatgtcaa tgagccgctc ttcaacttcc
     4741 cggaacagga ggccaatcac catgactaaa ctatatttgc tacttttagt tgcttgtctg
     4801 ttcatcgtgg catatatccc gcgcttgttc ccgatgcttt attttactca tcgaaaagtg
     4861 ccatcctggt tcagtgactg gatgaaatat gtaccggtgg cattatttgc ggcgttagct
     4921 tttaaagatg ttttcattac tcatgaacat ctggatattg cttggaatat caaaatagct
     4981 gcaatgattc tagtagcagg cgtggcttac aaaacacgtt cgatggcgtt gtcagttttg
     5041 acaggacttg cgtctgtctt tttgctgtct atgctttaaa ctgttttatt ttattaaaaa
     5101 cagagattgc gacataagtt gttcaaactg atctatccga actgcatttg caacagtcaa
     5161 ttctggatgc aagtttattc gtagggataa aaaagattag tgttggctta tgatgcaagc
     5221 tcttttttat tttaggaatt agagagggat ttgtccttct atgatttttt gctataatag
     5281 ggaagatgtt tattttatga aagaatagtt gagggaattt aaatgaataa aaaattgatg
     5341 caaggaacat tttggctaac ctttgctaat cttctatgta aagttttagg ggtagtctat
     5401 ttgattccct ggctaagtat gatggggaac aatcaagatg gtatgcttgc tacgacattg
     5461 tataatgttg gctatcttcc ttatggattg ttcctgatgc ttggtacagt cggatttcca
     5521 aatgcgattg caaaaaaagt agctgtagcc acaaaagaag gagataacgc tgcttgtcgg
     5581 ctgattttta gaagtacgat caatatcatg tttgtcatcg gtatcgcctc tgctgcactg
     5641 atgtatttat ttgctccttt actagccgga atcagtccga ttgctaacgt taataatggg
     5701 atactagcta tcagaagttt atgcccatct ttgattgcta tcccaattct tagtgcaatg
     5761 agaggatatt tccaaggaaa aaatgatctg cggccttatg gaacttcttt gattatcgaa
     5821 caggctgtgc gagtgatcgt tatcttggct ggtacttatt acttgcgcgt attgactgac
     5881 ggaaccatat tagaagctgt attgatcagt acagtcgctt ctttcttcgg tgggttagct
     5941 gcgattatcc atatgtatat cgtcggacgc agaaaagact tcttcgaact taaagatttt
     6001 ttgatttcca gccgttattt cgaaagagaa aaccgatctg cctctgtctc tatcattcgg
     6061 gaaacactac cgtttatttt tgtcggctca gtcattacta tcgttcaaat gatcgaccaa
     6121 gtaacgatga aaccactgct tcactttttc cgtccagaga tcgcagatca gcaactggaa
     6181 ttcttattca gccgtgcttc tgtcaatcca aataaattga cattgatcct gatctccatg
     6241 gtaggaacag tagctataag cagcttgcca atattaagca cgatgaagaa aaaagatcgg
     6301 atacaaatcg aaaaaacagt aggagatagt ttttcgattg cgttattgat tcttcttcct
     6361 tcattgactg gaatgtcttt attagctgga ccactataca cattgttttt tggctatgat
     6421 cctgaaagtg tcggatattt ccaaatggct ttgctagctt ctttgttttt ctctttattc
     6481 acgatcctat ctacgatgct tcaatctttg aatcaccatc gttttgcgat ccgattaact
     6541 gtagaagcaa ttatcctgaa agtcatcttc caagtaatcg gattaggatt agttggcgga
     6601 tacgggatga gcttatcaaa tggcttagca ttcggtattg tctttgttcg cggctatcac
     6661 tttatgtgca aagaataccg aatagcacct ttagcaaaga tcagcaattt tttcttaaaa
     6721 actttccgct ccaccctgat tatgcttgca gtatgttttg ccgttttctt tttgctgaat
     6781 cagcgattgt caatggaatc aaaaagctat gcaatgattt attgtgtggc tgtaggaagt
     6841 attggcggct tagtattttt attttctcaa ttcggaaaaa atgggtttcg aatgttgaaa
     6901 aatgttaaac atcatagcag aagcaaagaa taggttaact aatcgttttt tgctgtcact
     6961 tgaaaaacgg caactcgtat gattacgctt gtcgtttttc tattttccaa ttttagtttc
     7021 atatctaatc ttacttatga tatagttata aggtctggaa aaattctttg aaacgaggaa
     7081 attaaacaca tgaaacttgc agtagttaca gatagttcgg catatctgcc ggaacgtatc
     7141 aagaaccatc ccgacttatt cgttattcca atccctttga ttatggatgg gaaaatttat
     7201 aacgaaggaa tcgatatcga agcggatgaa tattatggac ttttaaaaaa cagtaaagat
     7261 tttcctacta cttcacaacc tgtcgtaggg gaagttttgg gtttatatga ggaattgaaa
     7321 gagaaagggt atgacacgat tttaagtatc catctttctt cagggatttc tggctttgtc
     7381 aacacattat ttgcgatgaa agatgacatc agcggagtaa cggtcattcc ttatgactct
     7441 aagatcacta gtatgccgat gggacacatg gtagaagctg ccttagactt aaatgatgaa
     7501 ggtcagtccc ttgatgaaat acttgcacat atcgatcgaa tccgagataa tacctacgct
     7561 tatctgattg tagatgattt gaacaattta gtacgaggag gacgtttgac aaatggtgca
     7621 gcgctgattg gcagcttatt aaaaatcaaa ccgatcctga cattttctga agggaaaatt
     7681 gttttattcg aaaaaatccg ttcaagtaaa aaagcatttg ctcgagctga agaaattatt
     7741 ggtagaagaa ataacgaaat acaaccgcca gtcaaacttt acgtgataca tgcaaataat
     7801 ttagaagtag cgattgcgga gaaagagaaa ttgcagaaaa aatatccaaa tgcagaaatc
     7861 gaaattggtc atttcggacc agtcattggt acacatttag gtgaaaaagc catcggcatt
     7921 gccatttcag cgcaataatt ccttttattc aaaaaacata ccaaaaatat tccaacatca
     7981 tcttggactt tctacatgtt actaatgagc gtgtaaaaga acgagatggt gtttttattt
     8041 ctatcaatga agctcttttt ctttgcaatc ctcacttcaa tcctctataa tgtcgcatat
     8101 agtttagtaa atgagtaaac tactaattta cttatttact aaaaataaaa ggatgaggag
     8161 ttaaaataat ggaaatacca acaaatttaa aacataaacc agtaatcgtt gtagaagact
     8221 atgataaagt tgatggaaga aatgcattaa atacggatgc aaaagggctt tcactgggcc
     8281 ttgctcagtg gaatgatcgt ggaaaagtcg atatctcagg caaggtatgg cgacatactg
     8341 gagaaaaatg gtcacgacag tcggaagaat taccaatcac acgtatttta gatttagcga
     8401 tcttagctgc acaaggaagt ttatacttcc aagatgctta tcgttatgaa aaattttatg
     8461 atccagaaaa tccagtcgta gacatcattg gattacaagg gaaccgaatg acggtagaag
     8521 tagataccaa aaatccaaag atcgatgaag acatcatttt gtattacgat acactacaaa
     8581 aagacggaga actgattggg gaacgattcc gtattttaaa acgtctttta gatgagctag
     8641 gatattaata gacagagatg atcaaaaggg gaagagaagc atggagaatt tctcactaac
     8701 agaaaaccga accttttcac ggctagaaaa gcaaatgatc tggaagaaac cgaaaagcca
     8761 tcggacaagt gcagaagaat ggcggatcgc ctccgaaatc aaagggaatt ggcaagatcc
     8821 tgaaatgaaa atcagtaacg tacttttgga aggagatgcc gggtcgggga agacacaatt
     8881 agccaaagca ctatctgctg atctgcaact tccctataca aaagtgacct gttttgcaga
     8941 tatggacaaa tcagatgttt tcggagcctt attacctgta gtagattcta ataaggaaca
     9001 agaccaagaa ttactagaag ctatttacca aacagataca cttgaagctg tactgacact
     9061 gatccagaac cattacggca ttgatccttt ttctgctaaa gaaaaattag cagagctagt
     9121 tcaaaggatc gaagctgatt atccagatgc aatccattac agctattacc cttctgaaat
     9181 cgttcgagcg attgaaaaag gatatttatt agagattcaa gagccgactg tcatccgaga
     9241 tgcctctgtt cttgtggcac ttaactcagc gctcgaacca aatgggatgt tgaatttacc
     9301 gacaggaatc attcgtcgcc atccagattg cgtcattgtg atcactacaa atcgaaatta
     9361 ccaaggaaat cgcccattaa atgaatcttt aagagatcgg atgcaacatg cggaaaaaat
     9421 ggatcttcct gccttagaag tcatggcaga acgtgcgatg gcgaaaacta tgatccaaaa
     9481 acaagaactt ctcttaaaga tggcagagat tatccgtttg ttggatgaaa ctgcaaaagc
     9541 taacgccatc aaaggtgtag caggaatgcg ttcttatttt tattgggcaa atacctataa
     9601 gcaaggacaa gatttactcc agtcgattta tccaaaagtt ttgtataaat tatccactga
     9661 ttcagatgaa ttggcaattc ttgaacaagc tttgtctgac agtggattgc tactggaact
     9721 tcaggacata ttgagaaaag aaaaatgggg tcatcctcaa tctgagcata caaaaggaag
     9781 aaccattagc gccgaagaag caaatgaacg aaacatcact gaaaatgctg aagaaattct
     9841 gcgcttagat gaaaagaaat cgttgtctgc tgaagaacca aatgagggag aagagaaaca
     9901 agaagaaaca atagaaaaaa acgagattcc tcctcaaatg caaaaagaac gcagtagcga
     9961 tatcgaagga aaaaatcaaa aagaacaaca aacaggagat gattctcaag aaatgtctgc
    10021 ttctgacatg gaagcacatg ataaggaaat caagaagcaa ctgaataaag aagcgcgaca
    10081 aatcatgaag gaaacgattc atgaaaaaga aggtctcatc gttcaccgac cagctttcca
    10141 aggaaaaacg ccggaagcac gtgagctaca catgcaagta atgccaatag tagaatcact
    10201 tagccggcaa attttagagt tactggataa tgaacaaaca gagacgtacc aaaaaggaaa
    10261 atacgaaggt cagcggttca atgcaagcaa agtagcatat ggtgatttga gaaattttga
    10321 caaaaagaac ccgccacatg agcaaccttc tttagcggta gctgtacgga tcgatgaatc
    10381 tgggtcaatg atacgggatg atcggatcga agcggcaaaa aaagcagcga ttgccattgc
    10441 cgaattttct aagaaagtcg atatcccatt gttgatttac ggagatacag cagaccgttc
    10501 gtcaagagaa aagacttctt tgttttctta taaagaattt gaagacggat ttcaatggtt
    10561 aaatgaaaaa ttagtcacta tgaaaccaag gcaaaacaat cgtgatggtg ctgcacttcg
    10621 tttagcagca gaaaaattaa acagacaatc cgcaacaacc aaactgttgc ttaatattag
    10681 tgacggtcag ccaaaagcgt tgccggatta tacaggcaaa aaagcaaaag aagatatcca
    10741 gtctgttttg aaagaatatg aacgtcaagg aatcctattt gtctccgcag ctattggcca
    10801 agacaaagaa gaaatcaagt cgatctacgg tgaaagtcgt tttgtagata ttactgactt
    10861 gaatgaattt cccaagcaaa tgattcaact gattgcccgc tatctatgat aaaataaagc
    10921 tcaacgaacg cacttagcag attcgttgag cttttcgttt ctagttaccg gtaacaagga
    10981 tacttctaaa atagaatcgg ttataatcaa gagggaccgt taaaggagtg aaacgatatg
    11041 atacaagaac aaaaatggtg gaaagaagca gtaggctatc aaatctatcc tgctagtttc
    11101 aaagatagca atggcgatgg tcaaggagac atcaatggga tccgtgaaaa acttaattat
    11161 ttgaaagatc ttggtatcgg ctttatctgg atcaatccga tctatcaatc tccatttgtc
    11221 gataatggtt atgacgtaag tgactatcaa gatatagaag agagattcgg aacgatggaa
    11281 gattttgatt tattgctaaa agaagcacat gaattaggga tcaaagtgat tatggatctt
    11341 gtcatcaacc atagttcgga tcagcatcaa tggtttgaag aatcgaaaaa atctaaagaa
    11401 agcccgtatc gcgattatta catttgggtc gacggggtag atggcaaaga accgaataat
    11461 tggacatcga ttttcggcgg ttctgcttgg gagtattctc atgaaactgg acagtattat
    11521 ctgcatgttt ttgcaaaaga acaaccagac ttaaattggg aaagcgagaa attgaaagaa
    11581 gaactattca atatgatccg atggtggttg gataaaggga tagatggatt tcgtttagat
    11641 gcgatttctc atgtaaaaaa agatgaatat tcagtaaaag ccacagagaa ccctttctca
    11701 ccctttcaaa atgtctcagg aatcgaagaa catttgaccg atttgaaaca tgtttttgaa
    11761 gagtacgata tcatgactgt cggagaagca agtggtgtca ctgcagaaga aggaccacaa
    11821 tgggtaggga aagacggtta tttcgatatg atattcgaat ttgatcatat ccatatctgg
    11881 cagcaagaaa aagaagggca attagatgtc cttaaattga aacttgcatt aagcgcttgg
    11941 caaacttcat tggatggtat cggatggaac gctttatata tggaaaacca tgatgttcct
    12001 cgtgccgttt cagtgtttgg agatactcgt cccgattttt gggctatgtc tgcaaaagca
    12061 atcgctatga tgtatttctt tttgcaaggt acgccattta tttatcaagg acaggaaatc
    12121 ggaatgacga atatgccctt tgaatcgatc gatcaagttg atgcagtaga ttcgaaaaga
    12181 ctttataaaa ggctgcttgc ggaaggtaag acaagagaag aagcattgga tatcattcgt
    12241 gaaacaacga gagacaacag caggacaccc atgcaatgga cttcggaaca atatgctggt
    12301 ttttctacac atgaaccttg gctgatcact aatccaaata ccaaaacgat caatgtagaa
    12361 caacaagaat atgaaccaga gtcggtcctg caattctata agaacatgat ccgcattaga
    12421 caaaccaata aaggtctgat atacggttct tataaagaat atcttcatga acatccacaa
    12481 ctttatgtct atgaaagata tttagaagat gaacaatatc ttattatggt aaacttaaca
    12541 gaatcacttg ctgactatga acttccaaaa gaagcagatc agtcctggac attactcttg
    12601 tcaaattcct ctagcggaga atttgaagct aaaggcatac tagcacctta tgaagctcgg
    12661 ttatataaaa caaacaagta aaacttcctc gttaaagttc attagttgga ctttgcgagg
    12721 aagtttttca gattgctctg tcaattactc atttcaataa ttcgctctag gaccaccgat
    12781 atatttagga cgagagcgaa gggctgtcac gtgagcttgt ccaagggttt tcaaacgagg
    12841 tctgacaggt acttggacat gtttgatatg cataccgatt gatgtgtcac cgatatcgat
    12901 accggcttgt ccaactacgt gttccacttc gacaggatca ttgaactggt caaaagcagc
    12961 aacagaacaa gcacctccag catgtagtgc aggaacaaca gagacaattt cgaaattttt
    13021 tgcttctgcc actgttcgtt ctactactaa tgctcggttc aaatgttcac aaccttggac
    13081 agctagcgca atttctttag gatccaacaa ctcttttaac gtacggatga tccattgacc
    13141 aacttccgca ctggaatttt ttccaatatg tcctccaacg acttcgcttg tgctgcatcc
    13201 taaaacaaaa agatcacctt ttttcaagtg tgcctcagcc aatatgtcat taactatttc
    13261 agtcaattgg tcttttaatt ctttttctga caacttcatt ttctaaaacc ttctttttca
    13321 attaatattg cttccgtgct tgaatacgtt gaagacttgc agttatagga aaggcaagtc
    13381 ccattccaat cagattttgg aagatgtttc ctggtataga agccactcca gccggccaat
    13441 catataaaat ccaaccggcg ataccatagc cgatgatcat aactaagctt cctaataaaa
    13501 gccctaatcc ccacatacga gtagaagatt tgctgaaaga accagcaaca tatccttgta
    13561 acccgtggat cactaatgaa aaaactgccc actgcggata tcctgaaatc aaatcgatca
    13621 atagaccact ggaagcacca acaactaatc cacccgttgg accataaaga aaagcagcgg
    13681 tatagatccc ggcctcgcat aatgtcacaa atccattcgt agccggtaca gggatcaaaa
    13741 ttgttaatga aagggcaact gtcaacgcaa tcagcatagc atagatcgtt aattgcttag
    13801 ttttcatttc tcattcctcc tttttgaata ccgccagacc agacacttcc ggtatgatcg
    13861 ttcatcagca cgccattaga aatacaatgg gcaacaaatt ttttactttc tgctactgct
    13921 ttggtaaaat ctgcccctaa cacaagattc gctgttattg cagaagaaaa ggcacaaccg
    13981 gccccgttta ctgttgtagt gtcgattttt ttatgagaaa atacctcata atgatcaatt
    14041 gaatagagaa gatccacagc ttcatctcca gccatccttg tgccgccttt gatcacaatg
    14101 ttttttgttc ctgaagcagt gagttcttta gcgagatgga tcatttctgt taacgaagag
    14161 ggaagagctt gaccagaaag taaagcagct tctgccaaat taggcgtaat catcgtaaca
    14221 agtggaaaaa gttcttcgat gagctgcgaa atatacaatt gattgatcac agcttcagtt
    14281 tctttgaatg ccaaaacagg gtctaaaatg attgggatat tttgttttgt ttttatgaag
    14341 tctcgaacaa ccatgattgc ttcacttgaa ttcaataacc caattttgat accatctaat
    14401 gaagtcattt tatcaatcga aactaactgt cgtttaagca attccaaaga tatagattcc
    14461 agcataaatc gctcattaat atctagtgcc cctatagaag tcaaggcgct tagtccgaat
    14521 agaccaaaat tctcaaaagt ttttaaatct gtttggattc ctccaccagc aaatggatca
    14581 gaaccgccaa ttgtcaagat tcttttcata acattttcct ccatgaataa gagtataacg
    14641 aaattttcaa aaaagaaaaa cagccaattg gcatattttt atctcatcca atttaaaaaa
    14701 acaaaaagaa aagaaagacc aaaatggatc tttctttcct taaatagtta ggacttttgt
    14761 gattttaatt ctttctcgat ttgaagccgt tgcactttca ttgtagctgt tcgcgggatt
    14821 gcatcaaaat cacggatgat agggacgttc aagcgaggaa gttcatggac ttgctcccac
    14881 catcgattcc aatccatttc tttatcagga gctaaagcaa taatcggttg tggagaatga
    14941 tttttatccc ttacaatcac tacttctgcc aaaaaatcta acgaatctaa taaaaagtct
    15001 tcaatagcta atgtactatt gatcgtttcg atcaaatcca cttgccgatc tttcaaaaat
    15061 aggtggccat gcttatccat aaaaccgtaa tctccgctat cccaccaatc gccataaaca
    15121 ttttcctgga accgtgcatc ttccttgtaa taagtcaaag cccgtccttt agacagtaat
    15181 tgtatatgac catctgtgtt ttcaggtagg acattcccaa ctgaatctgt aatccgagcg
    15241 cttgtcatgt cttccagtcc aacgcccata tctcttgcat cacttgtttt caaactctct
    15301 agtgtatgag ccttaagaat cattggtccg cattcacttt ggccatatac ttgaagaaat
    15361 accgcatcct gtgccatcga cgttcgcaaa aatgtcttca ttgtctggtt gtttatagca
    15421 tcaaaagtgg agtgatagta tcggataccc gaaaaagctt ctggatgctc cttagcagta
    15481 aaacgccact gaacaaagtt attcggatgg gtctctagtg caattggccg gtgagcttcc
    15541 aacatatgaa caacttttga actttgtgcg tttgctaaag gcatcatagg aaatcccatg
    15601 gacatcaaag aagagatacc aatattgaat ctggaatgca ctggagagat gtgaaaagcg
    15661 attaatttct tttccgaaat ttttgtgaaa actgtttttt gccatttcgt ccgccatccc
    15721 atagaatgag cagaatgaca aataagttta gggataccag ttgtaccaga agtatgggtc
    15781 atataagaaa tttcattttt gtctaaaaat gattgagaaa cagacaaagc aggttgttcc
    15841 attaaataaa gaatagg
//
LOCUS       DACDIH010000061        15538 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDIH010000061 DACDIH010000000
VERSION     DACDIH010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.061"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..160
                     /locus_tag="IV281_002054"
     CDS             <1..160
                     /locus_tag="IV281_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8781035.1"
                     /translation="EIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKV
                     NKMNLEVE"
     gene            160..1536
                     /locus_tag="IV281_002055"
     CDS             160..1536
                     /locus_tag="IV281_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8781036.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(1822..3444)
                     /locus_tag="IV281_002056"
     CDS             complement(1822..3444)
                     /locus_tag="IV281_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HAP8781037.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     PFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFT
                     KEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVK
                     GEREKQFAYSVHTSCDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCTISKNHQRVITRHVWGDLMDEVEHLRLTDLNKS
                     IYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKE
                     PLFFCSKIRNEVDKKLFQARVTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNIN
                     VYFTKARFKRLIAITVSSIVMFLWLFRMGVIP"
     gene            4096..5067
                     /locus_tag="IV281_002057"
     CDS             4096..5067
                     /locus_tag="IV281_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP8781038.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(5145..5381)
                     /locus_tag="IV281_002058"
     CDS             complement(5145..5381)
                     /locus_tag="IV281_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8781039.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(5350..5481)
                     /locus_tag="IV281_002059"
     CDS             complement(5350..5481)
                     /locus_tag="IV281_002059"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8781040.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(5557..5940)
                     /locus_tag="IV281_002060"
     CDS             complement(5557..5940)
                     /locus_tag="IV281_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP8781041.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(5973..6758)
                     /locus_tag="IV281_002061"
     CDS             complement(5973..6758)
                     /locus_tag="IV281_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAP8781042.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            6936..8036
                     /locus_tag="IV281_002062"
     CDS             6936..8036
                     /locus_tag="IV281_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8781043.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            8026..8850
                     /locus_tag="IV281_002063"
     CDS             8026..8850
                     /locus_tag="IV281_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAP8781044.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            8856..9662
                     /locus_tag="IV281_002064"
     CDS             8856..9662
                     /locus_tag="IV281_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8781045.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            9659..10489
                     /locus_tag="IV281_002065"
     CDS             9659..10489
                     /locus_tag="IV281_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8781046.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            10496..11527
                     /locus_tag="IV281_002066"
     CDS             10496..11527
                     /locus_tag="IV281_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8781047.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(11634..12907)
                     /locus_tag="IV281_002067"
                     /pseudo
     CDS             complement(11634..12907)
                     /locus_tag="IV281_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            13184..13393
                     /locus_tag="IV281_002068"
     CDS             13184..13393
                     /locus_tag="IV281_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781048.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            13443..14159
                     /locus_tag="IV281_002069"
     CDS             13443..14159
                     /locus_tag="IV281_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP8781049.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(14061..14581)
                     /locus_tag="IV281_002070"
                     /pseudo
     CDS             complement(14061..14581)
                     /locus_tag="IV281_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            14869..15522
                     /locus_tag="IV281_002071"
     CDS             14869..15522
                     /locus_tag="IV281_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8781050.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT         4692 a         2763 c         3377 g         4706 t
ORIGIN      
        1 agaaataatt aatatattaa agacattagc taaagataga aataaatgtg taatagttgt
       61 aacacatagt aaggaagtag cagattctgc ggatatcatt ttagaactaa gtggtaaaaa
      121 gttgaaaaaa gtaaataaaa tgaatttgga ggttgaataa tgataaaaaa tgcatttgct
      181 tatgtaacta gaaaaagttt aaaatcatta attatcatat tagttatttt atctatgtca
      241 actttaagtc tcattagttt atctattaaa gatgctacgg acagagcttc aaaagaaaca
      301 tttgctaata taacaaatag tttttctatg gagataaata gacaagtaaa tccaggaaca
      361 cctagaggtg ggggaaatgt aaaaggtgaa gatattaaaa agatatctca aacagatagt
      421 atagactcct atgtaaaaag aataaacagt gttgcagatt tagttgatta tgatattatt
      481 gaaactcaag agactcttgc gaatcaatca cctgaaagag caaagaattt taaaagaaca
      541 gttatgttaa ctggggttaa cgactcaaca aaagaaacaa aatttgtatc agaagcatat
      601 aaattagtag aaggaaagca tttagaaaat gaagataaaa ataaaatctt aatgcataaa
      661 gatttagctg aaaaaaataa ccttaaagtt ggagataaga taaaaataaa atctaattta
      721 tttgatgctg ataatgagaa aggtgcagat gaaacagtag aagtagaaat taaaggtcta
      781 ttcgatgggc ataatagcgg tgtagtaagt gcagcacaag aactatacga aaatacatta
      841 atcactgata ttcatacagc tgctaaagtt tatggtaata ccgaagatac agctgtatac
      901 caagatgcaa cattctttgt aaaaggtgat aagaatcttg atagtgtaat aaaagatctt
      961 gggaaattag atataaattg gagagaatat aatttgatta aaagttcatc aaattaccct
     1021 gcattacaac aatctatatc aggtatttac tcaatttcaa ataaattatt ttttggctca
     1081 ttaatatttg caggagttgt agtttcatta ttattattct tatggatgaa tgctagaaag
     1141 aaagaaatag cagtattact atcattaggt atatcaaaat tagaaatttt tggtcaattc
     1201 ctaattgaga tggtatttat atcaatcccc gcattcgttg gttcctattt cttagctcaa
     1261 tatacagctg acaagcttgg gaataatata ttgaataagg ttactggtga tatagctaaa
     1321 caaatagcta aacaatctgc atctagccaa ttaggtggtg gggcagaagc tgaaggattt
     1381 aacaagacat tatcaagttt agatatcaat gtattaccta aattcatgat ttatgtagtt
     1441 atatttatga gtttagtact tcttgtatca ttgattattt cttcattcaa tatattaaga
     1501 aggaatccaa aagaattatt aattgacaat aattaaaatt ttcggtgctt tttagcacca
     1561 aaaatgagag tgtaatataa accgtgtaag taaccatcaa tcctaggcgg gtggttgctt
     1621 cacggttttt atttttctaa aataagatca agaggtgatt acacgatggc tagaaagaaa
     1681 agaaatcctg atgctgagaa gttagctgaa tccattttga atacttatca acccgaatct
     1741 gtagaggata tgcaagatgt tttaaaagat gtgtttggtc ccttatttga aaaaatgctt
     1801 caaggagaat tgaataacca tttagggtat gacgcccatt ctaaagagcc aaagaaacat
     1861 gacaatcgac gaaacggtta tggcaataag acgcttaaaa cgagctttgg taaagtaaac
     1921 attgatgttc ccagagaccg ggaggcttct tttgaacctg agttgattcc taatagaaaa
     1981 cgagatgtct ccgacatcgc agactgtaga caaaagctgt tccaaactcg taactcgagc
     2041 ttggaacagc tttttatcta cttcgtttcg aatttttgaa caaaaaaata aaggctcctt
     2101 gcctttccat agccaggtgg ctaatttctt taaattcatt gcagcaaaca ctagcatcgt
     2161 atgcgtcgcg actttttcca agcctcgata cttggtccaa cgcatcccat gcttttcttt
     2221 tgcatccgca aatattcgtt caatcgtttg tttccgtttt ttgtaaattg atttattcag
     2281 atctgttagg cgaagatgtt ccacttcatc catcagatct ccccaaacat gtctcgtgat
     2341 gactcgttga tgatttttac ttatggtaca ttggtttagc agaggacagt tcacacattc
     2401 ttttggattg gatttgtatt ctcggtaccc ttctcgagta accgttgaaa aagcgagcat
     2461 ttgatcttgc ggacaaatat aacaatcaaa atactcgtca tagagaaact cattttttga
     2521 taatagctcc tttttccctt tgggccttga ataaggcaaa caaggtcgaa ggttcaaatg
     2581 agtcaaaaag tgaacaaact taggtgtctt atacccagca tcagcgacaa cacaattgat
     2641 ttcaggaaaa agtctcttcg attgtttgac gagctgaaag gcgacttgac tgtcatgaat
     2701 atttctagga gtgatgattg tgctaagaat aaatccattg tcgtcgcatg aagtatggac
     2761 agaataagca aattgttttt ctcgttcgcc tttgacgtag tagccgcttt ccggatcagc
     2821 tttacttatt tttctttctt tcagcttcac ttccttttct aagtccaaag taaaagggcg
     2881 ttttccagcc tctattcgtt gacgattgat ctcatcttct aattcatcct gataggcttt
     2941 cgcttcatca tgggtcattt ctttcgtaaa cttatgttta ttggcgttag ctttgatgtg
     3001 ggtagaatcc agatacaaat tatcctctgt tacaaaacct gctttaactg cttgttctaa
     3061 aatataggca aaaatatcct caaacaccgt cgtttctcta aatcggcgaa cataattctt
     3121 tccaaaagtt gagaaagggg ggattttctc ctcgaaggag tagcctaaaa accagcggta
     3181 agcgacattg gtatcgactt ccttaatggt ttgtcgcatg gaacggatac cgaataaata
     3241 ctgaataaac accaatttga tcagaatcac tggatcgata cttgggcgac ctaaatcgga
     3301 gtaagtggct tcgacgattg gataaataaa ttcaaaatca agcgcggcat caatctttcg
     3361 aacgagatga tcttgaggaa ctagatcatc aatcgtaagc atcgcaaatt gatttctacc
     3421 attcgtattg tttttagaca tcatagctat cactccttgc attcgttttc ataaatctat
     3481 tataaaagaa aagactgtag acatcatcca taaaatggac tttgtctaca gtctggataa
     3541 cgtgattttt tgctgtaatg acaaggtttt agctgtttca gataccatgt atccaaaaat
     3601 agtttattga gaaaatagga gagtatttga tactaactag taggaaaaaa tgagataaaa
     3661 agtaaatgga atcatttaag gtaatctgaa ataaaaaata tacagccctg tggtacttta
     3721 cctacaaggc tgtatatatt ttttactctc agcgtttttg actccgtgtc tgactccgtt
     3781 ttattgattt tgaatgttat tcaatgacca tttaacaggt caaatttgat gtttttgatt
     3841 ccgtatgacg ccgtttgata tctagtgata tagtgatttg agtctacatc gaataaggga
     3901 ataaaccaat aaaaaatgat ttagaatatt aaaataacaa aaaagaggac tacctgtatt
     3961 agtcctcttt tttgaccaga ttttgaccag tctattttac atcctagtat atttatatga
     4021 gctaatttta tgattaaatg gtaagttttt ccttgtgtgt tttagtttaa actagtgtgt
     4081 aattcaaaaa tctatatgga aggaacagta ttaagaatag aacagggttc tctacatgat
     4141 ggtgctggtt tacggaccgt tgtttattta aagggctgcc cattacgctg tgcctggtgt
     4201 tcgattcccg aatcacaaag caagcagatt gaaaaaggat ttggccagac catgaccgct
     4261 gaagaggtaa tggatgagat tgagaaggat gctgtttttt actttcactc ggatggcgga
     4321 gtgaccatta gcggaggcga ggcactggtt caggcggatt ttgctaaaga aattttgcaa
     4381 aaatcgaaat acattggaat caataccgta ctggaaacca gcttttgtgg tgcttataat
     4441 gaaatccaaa aggtagctcc ctatgtggat acattgtttg tggatgtaaa aatgtttacc
     4501 agcaagctcc acaaacaatg gaccggctta gataaccaac aaattttgaa gaatattcgt
     4561 cgctttttaa ttgagtatcc taactgcgaa gtgcgaattc gagttcctgt ggttccaggt
     4621 atcaatatga acctgaccga gctgctgacg attgcttgtt ttgtggcaga tcttgatcgg
     4681 ttcgtaccgt tagaattact accgtaccac tgctatggta tgcacggtta ccaagcattg
     4741 ggactggagt atccgttggc agacacgcca gcgccttcac ctaaagaaat gtttgcattg
     4801 gcagatcaac tggctcgcac ggttcctcat ttgctggtca cagctttaac caaaacattt
     4861 atttatttat tgaatccaag ctttatctta tggataaatg aaagacactc actagctgca
     4921 gccagctcaa gaatagagct tttgctgaag tcagtgagtg tctttttacg atgttttttc
     4981 gaagttaatt tgtcgaatga acactggctc aatcggtaca tcttcactgt ttgtcggctg
     5041 agctgcgatc gctgctacga tttctaaccc ttggtatacc tgtccaaaaa cagtataacg
     5101 accatccagc tccggtagtc ctccaagctc caagtatgct tgctttacat tagcaggtca
     5161 gcctgcgcct ttgtgttttg cacaataaaa aactggttgc cgttggtgtg ggggccgtgg
     5221 tcccctaagg ccaaggcgcc attaaaatga taaagtccgc gatgcacttc atcagcgaaa
     5281 aagccctcat agctggaaac agcttcatca ccagaaccat ccaaagcgcc accttgaata
     5341 acaaattcct taatgacacg agccgaaagg tgtgttgtca taaacgcctt gctcagctag
     5401 ggagcgccag ttggctacag ccaggggagc ttggtcagga aataagcgga gacgaatttc
     5461 accagcatcg gtgaccagca cacaaacttc ttcctgctgc ggtttttcta attgaaagag
     5521 aggaaggttt gcaactgaaa cgccttgatc ctgccatcaa atatcgaaaa tccgccactc
     5581 gaccttcgcc tgatcaccaa ggcgagccat tcccaataaa acggtatagg cggtttcatc
     5641 cgcttgaaag gtcaattgca gctgtcctcg taaatcgttg tagtcatctg gggcggctac
     5701 ttgataaaaa agatgaaact gcgcatgcaa atattccaaa ggctgatctt taaaggctgc
     5761 aaaataagcc tgaacttttt cccgctgcaa gctgtcagcc accaaaaaat tttccagttg
     5821 ttgttttgga acggctgcta acaaggatcg gcaaaccgcc tcaattttca acgcatccca
     5881 atgtcgcgca attaaataat ggatgggtgc ctcttgattg tagtcccaca ggttgctcat
     5941 ttttttcacc actttcttct attatatagg aatcacaaaa tcggcagcgg cgtcaggcgg
     6001 cgttcttttc cttgaatgac tagcaacttt tctacctgac agtctgccaa cagctgactc
     6061 atttttttaa agcccagctg ataatcttcg gcaacatgag catccgagcc taaggtaaag
     6121 agctttccgc cgacactttg atacaaagga acggcataac ggtaaaggtc ttcgttgccg
     6181 tatttcaaga agctcttggc atttaactcc atcgccaatt ctcgttcaac aacctttttg
     6241 aaaatcgtta gcagctgagc ctcaaaatgc tccgcgaatt cttccgctgt gaaggtgaag
     6301 cggcgtaatc cataatcaaa gtgagtcaaa atatctcctt ctggaaaatc cgtcaagacc
     6361 tgcaacatct gatcaaaata ttccttggta gtttgaatgg gatctttggt taaaaccaca
     6421 tcgtccatat aatcaaagcg gccattttga tgaatactta ccagctttaa atcataagga
     6481 tgctgagcga ggtaatccac gatccgctct tcttgtcctg gaacaacgcc aatctcgata
     6541 cctcgcagaa atttggtgga ataggtttct tttaaagagt ccagtttgtt tatataggct
     6601 ggataatccg gaatgtcatc ttttccggta gatggattgt ttaaatcaaa gtgatccgta
     6661 gaaacgaaaa actccggctg ataggctaaa tagttttcaa atttttcctc tgaatcaaag
     6721 gaaaaatacg tgtggagatg ctgatcataa tactgcatag ggagcctcct tttttctact
     6781 atcataccgc aaaagcttta gaaggttaac gggaattcgt cgaaatttac aaaccatata
     6841 caacctttac attgttcatg aaaccttttc aagaaactga ttctctcatt acaaagcttt
     6901 gttagaatgg aattatcgaa aggggagaag aatgaatgaa aatgaatgaa aaaattcgta
     6961 acatgactat cggtttattt tgtgcagcaa cagtaagtat cctgctgaca ggctgcggct
     7021 caggtgaagc aacgaagcaa tcggctgcgg cagacgactc tttagatacg attatagaaa
     7081 aagccaagga agaaggagag attgccagct tgggaatgcc ggatacttgg gcaaattggg
     7141 taggcacttg gaacgacctt gaaaaagaat acggcttaag tcacgtggat acggatatgt
     7201 ccagtgcaga agaattggct aagtttgaat cagaaggaaa aaatggaacc gccgatattg
     7261 gtgatgtcgg tatcagcttc ggtccgttgg ctgaaagcaa agagttaacc ttaccttata
     7321 agaccaacta ttgggaggaa attccggatt gggcaaagga tgatgatggg gattggttgt
     7381 taagctatac gggaaccatt tcctttatca ccgataaaga gaatgtgaag caagcaccga
     7441 ctagctggaa ggagctgcaa gagggtgact atcaagttag cgtgggggat gtgacgattg
     7501 ccaaccaagc acaatttgct gtattggctg cggcgattgc caacggaggt gacgaaaagg
     7561 atattcagcc aggtctgaat tactacaaaa aaatggcaga aaaaggccgt ttgtccaccg
     7621 ttgatgccag cgtagcaaat cttgaaaaag gtgaaatcga tgtggcagtt gtttgggact
     7681 tcaatggact aaattatcgt gaccaaattg actccgagcg ttttgaggta actgtgccaa
     7741 cagacggctc catcattagc gggtatacaa ccttaatcaa taaaaatgcc cctcatccca
     7801 atgcggcaaa gctagcacgg gaatatattt tatctgatga agggcaaatc aatttggcaa
     7861 agggctatgc gcgacctatt cgcaaggtag agctgcctaa agaagtagct gaaaaattac
     7921 tgccggagga tgcttatgaa gcggcggtgc cagttgaaga caatgaagct tggaacaaga
     7981 cctctgtaga gcttccagag ctttggcagg cggaggtact gacctatgca aagtagactt
     8041 gacaaactga ttgtagttat actagacggc tgtcgctacg acacagccgt cgcccagctt
     8101 gggttcatga atcatttagt tgagcaccag caagcaagct tattgaaggt aaaaagtgag
     8161 ctgcccagca attcacgtcc gttatacgaa gtactgatga ccggcgttcc agcctatgaa
     8221 aatggcatct atagtaatta ccatgctcag cgatccaaag agtggtcgct gtttgaatta
     8281 gttaaggaag ctggcggaaa aacaggagcc gccgcttacc actggtacag cgagttgtac
     8341 aatcaagccc cctataatcg tttggttgat cgcatccagc atcaggagga gcgactgatt
     8401 cagcatggca tcttttatag tgaggacact taccctgatt cgcatttatt tgcggatgcc
     8461 cattatttag tgaatcagta tcagccagac tttttggtcg tacattcgat gaatattgat
     8521 gatgtcggtc acaaatttac cgccgattct catgaatatg cagcagcggt taatcaggcg
     8581 gacagattat tggctattta ccttcctgaa tggctgcaaa aaggttatca agtcgtggtg
     8641 actgcggatc acgggatgga tgcgcacgga ctgcatggcg gcagcttggc tgcacatcgt
     8701 gaagttcctt tttatttgat ttcaaataaa cgccaaaagc tgaaggaaca aactgtacca
     8761 cagctgctgg cggctccatt gttttgctgg ttattaggaa ttgcaccgtc agagaaaatg
     8821 caggacattc aagaattgat gaggggatga aagagatgaa aaaacgcttg gttctggttc
     8881 ctgcctcctt tatcttctgt gcatttttac tgctgccgct gctttttatg gttctgtgca
     8941 gcttgcacag tgatttaact aaccagtgga cattggccaa ttacagtgat attttcagca
     9001 acaaatatta cacacaggcc tttgttaaca gcgtactgat tgctgtcagc tccagtgtga
     9061 ttgggttaat cgggacgttt attttatgtt tatgcttaat gaatctatcg actaaattgc
     9121 aggagaaaat cacctttgtt tccaatttgg cagctaattt tgctgggatt ccgctggcat
     9181 tctcttttat tattttgttg gggaatgtag gtgtgctgaa aatgattttg ccatttctag
     9241 cggacttcaa tttgtattct tggtggggat tgaccatcac gtatacctat ttccaaattc
     9301 cgttaggggt cttgtttttg tatcccagca tgaaggaagt gaaaaaggaa tggctggagt
     9361 ccgcacaatt gctgggcgct tcatcagcgt atgcttggtt caaggtggct ttgccttttt
     9421 tgcgaccatc gattgccagc acctttgtca tcttatttgc taatggcatg gggacctatg
     9481 aaacagcgta tgccctgacc agcagtaatg taaatctttt aacgattcga attgcggctt
     9541 tagtgtcggg agatgtgttc gccaagccta atttaggcag tgcgttggca gtcgctttag
     9601 gtgtcctgct ggtaacggca atgttgatta ttcaaaagaa aggcaaggtg gtgcagacgt
     9661 gaaaaagtta ataaaaccga ttttgttctg ctttgtcctg tattacttgc tgccgattat
     9721 tggaacgatt ttgtatgcca gctccactaa atggtcaaaa tcgcttttac ctagtgattt
     9781 taccttgcaa tggttccaac agctgctgac ggacagggaa tttattgctg cggtgggtcg
     9841 ctcactttta ttagcaggtg tggtcttggt gacgattctg ttgttgatga ttcccaccat
     9901 tatttggatt catttgtatt tccctcgttt gaatcgttgg ctggaaaagc tgctcctgct
     9961 gccgtatgct ttaccaggag tgattttggt aacggctttg cttcgaacgt atgctgaaac
    10021 tggtatccca atgtttgtag tgttggtagg tgcattgttt attacggcgt tgccgattgt
    10081 ctatcttagt cttaacaacc agatgcgctt aattaatctg aaggaattag tggatgcagc
    10141 agaaaccttg ggcgccccga tgtctacgat tattatccag gtgctgtttc caaacatccg
    10201 tatcggtgtg accctagttt ctctgatgat tttttcttcg gtttttgggg agtacatgct
    10261 gacgaacctg ttgattggcg gtcgatttca gacggtacgc atctatatgc tgcggcggat
    10321 gaacgaaaac ggccatttgg ctagcgctgt gatggttttg tatttgctgt ttatggtggg
    10381 aattgcttta actaccttta tgctgactta tcgacaaaag caacagctgt cccttaagca
    10441 gacgagggaa gagaagcagt cgaaaaagaa gcccttaaag gagttgtgac ggaaaatgtt
    10501 tttagaaata catgatctca atctgttttt tgatgaaaaa cagattttag ctattgatga
    10561 aattcagatt gaaaagggtg agctggttac tttactaggt cccagcgggt gcggaaagtc
    10621 tacgttgctg cgctgtatca cgggattgga acaaccaaag agcggtgaga ttgtgctgga
    10681 caatgagaat attgccgata agcctacgaa ggatcgcaat atcggctttg tttttcaaca
    10741 gtatgcattg tttccaacta tgactgtttt tgaaaatgta gcctttggtt tgaaggtaaa
    10801 gaagctgagt cctgaggtga ttcaggaaaa ggtttttgag atgctatcat tggtggatat
    10861 gacggagcag gcagataaaa atgtccagtt tctttctggc ggtcaacgac aacgggtggc
    10921 actggctcgc tcattggtca ctgaaccgaa agtcctgctg ctggatgagc cattaagtgc
    10981 tttggatgcc cgaattagaa aacagctgca gcgggatttg cgcgctatcc agcaatcgct
    11041 agggatgacg atgatttttg tgactcatga ccaagaagag gcgatgcgaa tcagtgatcg
    11101 gatttttgtg atggaagctg ggcgggtggc tcaggtttcc acctcgaagg aattgtatca
    11161 gaatcctcag agccgatttg ttgcggaatt tatcggcaac tataatcgtt tcgaatggta
    11221 tgagctgaat cagtataccc acgagttttc cgctaagggc aatcgctgca tttattattt
    11281 aagaccggag ctgattcaat ttgaaccggt tcctcagggg gttaagattc cggttctatg
    11341 gaaggaatca tttattttag gcaacattca acggtatgtt tttcaaacgc tggagggcaa
    11401 agaaatttta gtggatcggt tgaacgatcg ggaagagaaa attagcgatg ccttatacat
    11461 tcagctgaaa gatattctag caatccctgt ggaaattgat actcagcatt ttgcacaaca
    11521 aagctagtgt atagtcggca aacatttctg gctctttgtc aaataggact gatagagtga
    11581 tataagaaca tctaaggtag aattgatttt ctgctttaga tgttttttta tgtttaataa
    11641 gtgatcagat gattgattaa gaaaattctg gttctcatcc taacccgtaa gctattcgtt
    11701 tcaatgcttt gatgtgggta tgcagatttt ctattttgcc atttgagtaa ggcaactcta
    11761 aggctaaacg aatgccggat tcatagttta taaacgcttt ttttatatga tgcagttctc
    11821 ttggaaccga cttgggcatt gtttttacaa gctgaaagaa gatgtccgct tctttgtcat
    11881 aaaaagcttg aagaatttct tgatagtaag cataagctat tcgtagttca tcagaagtag
    11941 aaagtagacg ttctacgact tcactttcgg ttaaataggc ccaaccaaaa ttacgccatt
    12001 ttgtccattt atcataaaca agcgtgtcct gtcgtttgat cagaagtcga gataaagcct
    12061 tcacttgtcg atagtgtttg gcttgtattg gatcgtttcg gtcgaagctt ttcatgactc
    12121 atagtcaaat atccttaaaa ctgcgaatga ggtgcttgac gatgtgaaag cgatccacaa
    12181 cgagtttggc atgaggaaag cactctttta tcagcaaagc ataagaggcg ttcatatctg
    12241 ttacgagtat ttttacgttt aaacgtgcac gtcgttcaaa ttgcataaaa taagaaatga
    12301 gatctttctt cttacgtgaa ggaagaatat ccagtaattt cccagtatcg ccatccatac
    12361 aagaaaaaga atagggaccg acttgatttt tcaatgaacg aaattcatct accaatgaac
    12421 agcgaggcaa ccaatttatt ttcacgtttg tcatggggag cagagaagcg agtgttcgcg
    12481 aaacggttga ccaagaaacg ccgcaatgtt tagaaatcgt cgtcattgct tgtttttcag
    12541 tcaattcctc caatatttta taatgtactt gtttggcgat aaaacagttt ttttcaacaa
    12601 tgtaggtatt cgccgtaaaa taggtgtgac agccacgaca gaggtaccgt tgctttgcca
    12661 aagcaagata agtccgttga ttcgcacact tattgagaag gaccagcgat tttttctttc
    12721 cgttcttaac gatcagccca tgttgattca cctgtttaca ctgaggacag ggctgaatcg
    12781 ttttaatgag cgtggcagaa acaacgatac tgttgattga atgtattttt ttcttctcga
    12841 catgagtgat ttgaatgttt ccatctttga ttcctagtaa tagtttgata agatgaagat
    12901 caggcataca gaacgctcct ttgattgtgt tgtggtgatt caattctaac ggagcttctg
    12961 tatgttttgt ttttttatgc tcaaaaggac tgatagaatg atttactatc agtcctagaa
    13021 attatagcgc cacatttctt tcacctttga cttttcagac ggcagttttt atgctgatac
    13081 tgtagagaga gcaggacaca gcttttgctt gttttgggta agggggaaag aagatgacca
    13141 aagcacttat ttttgatttt gatgggacga tgattgacag tgaatggatg ttttacgaga
    13201 gtattcatca ctatttactg aagcctattt actggagcag ttcggctata aaattccttt
    13261 tgcagactat caacaaggtg ttgggacggc agcagaactg tccttttatg aagggataag
    13321 tcaagcggtt gggagaccag tggatgctga acattttgag cagcggatca gcagcactgg
    13381 aaaatcggag tagtctcaat tctgaactgc gggagctaga ctattttttt gaagcacatc
    13441 cgatgattaa ggaaaaaatt gattcctttg tgacgatcga tgatcttcag gtcggcaaac
    13501 cagatccaga aggttatttg ttgtgcttga aacggctgag ggttgaacag gctgttgtag
    13561 ttgaggatag tccagtcggt gcccaagcag caccgcatat gacagttgat gtacaagcgt
    13621 tggatgcaga ttttttcgcc tttagcgggc ataaaatgtg cgggccgaca ggaatcggtg
    13681 tattatatgg caaacgtcaa ttgttagaac aaatggagcc agtcgaattc ggcggtgaaa
    13741 tgatagattt tgtcaatctt tacgatagtt catggaaaga gctgccatgg aaatttgaag
    13801 ctggtactcc gaacattgca ggagcaatcg attacttgaa tgagattggt atggagaata
    13861 ttcatcgtta tgagcaagaa ctggttgcct acgttttacc taaattacaa gcgatcggcg
    13921 gtatcaccat ttacggaccg caagatcctg aacatcatac tggcgtggtt gcttttaatt
    13981 tggatggcat ccatccccgt gatgtggcga ctgctctgga tatggaaggt gtcgctgtac
    14041 gttcaggaca ccattgtgca caacggttca tcgacgattt ctgtttgact aattggtcgt
    14101 ttaaacagtc gatgataatg ccgtttgagc ggctccaatg tcgtggaatc tttgagtaag
    14161 agtttccaat atcgcttgag ccgacggtac ttcttctgat tttcagatta cgtgattgat
    14221 tttcgtacag ttctatgaga atcgcctgct tcagttcctt tgttagttga tgatgttcat
    14281 caactaagtc cgttttcgca gaaaaggtct gcccacaatc atagcataga aaacgggtac
    14341 gttttaattc taaaatagtc aacttttctt tgactttcaa catctgagta cgtgtcgtat
    14401 aggaaccgtt gcggataata cgaatcgatc cacagtgttc acaatgggaa ggtgagtcat
    14461 ccaatctccc tttaataaac tgcgcttgaa cgcctttata ttctttttca atcagccatt
    14521 cttcatcaaa aaataaatgt ttatctgtta atccgagtaa ttttctagta tgattatcca
    14581 tagagcgact cctttggtag ggtgatgttt tggtcgacta aattttacca aaagtgagtc
    14641 tctttttgag ttgaaacgaa gaaaaagtgt tagtagaaaa tcgccttcga ttttccaaca
    14701 acacaaaata ttatagaagc cttttttcta aaggatttca aggtgtctgt ttgcatttat
    14761 taaaaaaaag aaaaagtatt ggcaacagaa ggcgtcacca acactagata ttatagagcc
    14821 ataatatgct gtataatatc tagtgtatag gcttaagtgg aggtatatat gaaaatatta
    14881 actgttgaag atgataatat gataagagag ggaataagtg aatatctttc agaatttgga
    14941 tatactgtta ttcaagctaa agatggaaga gaagccttgt caaaatttaa tagcgatata
    15001 aatttggtta tcttagacat tcagatacct tttataaatg gtttagaagt gttgaaagaa
    15061 attagaaaga aaagcaattt accaattcta atcttgactg catttagtga tgaagaatat
    15121 aaaattgatg catttactaa tttagtagac ggatatgtag aaaagccatt ttcattgccg
    15181 gtcttaaaag ctagaataga ttctttaatt aagaagaatt atggacattt agagaagttt
    15241 gaatataagg atctatcggt taattttaat agctatacag ctaaaataaa tggtgaagaa
    15301 atagatgtaa atgcaaaaga actagaagta ttaaaatgtt tattggataa taatggacaa
    15361 gtgttaacaa gaatgcaaat tattgattat gtatggaaag atagcgaaga aactccttat
    15421 gatcgagtaa tagatgtata tataaaagaa cttagaaaaa aattacaatt agactgtata
    15481 actactataa gaaatgtagg atataaattg gagagaaaat gaaaaaatta aagatatt
//
LOCUS       DACDIH010000062        15442 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDIH010000062 DACDIH010000000
VERSION     DACDIH010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.062"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(64..1173)
                     /gene="rpoD"
                     /locus_tag="IV281_002072"
     CDS             complement(64..1173)
                     /gene="rpoD"
                     /locus_tag="IV281_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP8781051.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1197..3038)
                     /locus_tag="IV281_002073"
     CDS             complement(1197..3038)
                     /locus_tag="IV281_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP8781052.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3220..5613)
                     /locus_tag="IV281_002074"
     CDS             complement(3220..5613)
                     /locus_tag="IV281_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8781053.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5720..6580)
                     /locus_tag="IV281_002075"
     CDS             complement(5720..6580)
                     /locus_tag="IV281_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP8781054.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6639..7271)
                     /locus_tag="IV281_002076"
     CDS             complement(6639..7271)
                     /locus_tag="IV281_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP8781055.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            7480..8199
                     /locus_tag="IV281_002077"
     CDS             7480..8199
                     /locus_tag="IV281_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8781056.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            8225..10816
                     /gene="mprF"
                     /locus_tag="IV281_002078"
     CDS             8225..10816
                     /gene="mprF"
                     /locus_tag="IV281_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8781057.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11004..12191)
                     /gene="tuf"
                     /locus_tag="IV281_002079"
     CDS             complement(11004..12191)
                     /gene="tuf"
                     /locus_tag="IV281_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8781058.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12559..12915
                     /locus_tag="IV281_002080"
     CDS             12559..12915
                     /locus_tag="IV281_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8781059.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(13156..14160)
                     /locus_tag="IV281_002081"
     CDS             complement(13156..14160)
                     /locus_tag="IV281_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP8781060.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            14326..15177
                     /locus_tag="IV281_002082"
     CDS             14326..15177
                     /locus_tag="IV281_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8781061.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            15194..15439
                     /locus_tag="IV281_002083"
     CDS             15194..15439
                     /locus_tag="IV281_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781062.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT         4482 a         3210 c         2730 g         5020 t
ORIGIN      
        1 tttttttctt ttaaaataaa ccaaacagtt ttactgattt ctcttctggt caatagtcca
       61 cgcttattct aagaaatctt ttaattgttt tgaacgagaa ggatggcgca attttcttaa
      121 tgctttagcc tcgatctgac gaatacgttc ccgagtaaca ccaaatactt tccctacttc
      181 ttccaatgtt ctcgtccggc catcatctaa accaaaacgt aagcgaagga cattctcttc
      241 ccgatcagtc aatgtatcta gtacatcttc caattgttct tttagtaact cataagctgc
      301 gtgttctgct ggacttgttg cttcctggtc ttctatgaaa tcacctaagt gagagtcatc
      361 ttcttcaccg attggcgttt caagggaaac aggttcttgc gcgattttca agatttctcg
      421 gactttttct gttggaagat ccatttccgc accgatttct tctggcgtag gttctcttcc
      481 aaggtcttgc agcaattgac gttggatacg tatcaattta ttaattgttt caaccatatg
      541 aactggaata cggatcgtac gtgcttggtc agcgattgct cgagtgatgg cttgacgaat
      601 ccaccatgta gcataggttg aaaacttaaa tcctttgcgg tagtcaaatt tttcaactgc
      661 cttcatcagt cccatgtttc cttcttggat caagtcaagg aactgcatgc ctcgtcctac
      721 ataacgttta gcgatactta ctactaaacg aaggtttgct tctgccagac gctgtttcgc
      781 ttcttggtcg ccttcttcga ttttcaatgc taacgctact tcttcttccg cagttaaaag
      841 ggagacacgg ccaatttctt tcagatacat acgaaccggg tcattgattt ttacaccggt
      901 tggcgcagaa agatcttctg tttttgcttg ttcagctttt ttctcagcac tcttcaagct
      961 gtgaattgat ggatcaccat tttcatccac cacactgata cctgcgtctt caatcttttg
     1021 gatcaatttg tccatttcat ctgcattcaa agtgaatggt gtagcaagct gattggataa
     1081 atcatcatat acaacttgtc gtttgggttt gttttctttg atgaaagctg ctactgctgc
     1141 ttcatacgtt tttttatctg ttccatttgc cataaaagaa ggcccccctt gagtagctat
     1201 ttggcttgtt ttaactgttt cgttaaattg atgatttcaa tcgtcaattc aagctcaagc
     1261 tgtttgtttc cagttcgtct ggcttcttgc tgcatttgtt tttttaatgt tatttcatca
     1321 gctaagccag atttgctgat cacatctagc aagtcttgga tttctcgtgg cgaactttct
     1381 tctgccaccg gtaaatattc gatttccacc accaaccgcc ttaaattttc atcttgcaaa
     1441 aagtctaaaa actctgcaag gttgaattca taatgttgtg agacatatgc atcgaataaa
     1501 aaataaactt ctgcatatgc atcatgaata aactgaaatt ctcgttcttt taaaatatta
     1561 cggacagaaa cttctttaaa tgcacgatat aatagaatct gctctgcctt ttctacttgc
     1621 gtcaaaggtc tctttttccg tgcagtttct tgtctcgtaa cctgagtgtg ttgactttcc
     1681 tgaggttctt gacgattttg ctgatttgtc cttcgaagat cgcgcagttg tttttgtatc
     1741 gtctctctgc cgatctgaaa ctctgttgac agttgagata gatacatatc ttgttcgatt
     1801 ggtgaaacaa ctcggctaag ttcaactagt aattcttcga ggtattcgat cttgtctttt
     1861 tcattatcta aatttttccc ttgttttaaa taacgtgatt taaaagtaaa gactgtttcg
     1921 cgcccatgag ccactaattc ttggaaagat gcttctccat acttacggag atattcatca
     1981 ggatcaagct tttctggaac gactacgaca gaaagtgcta atcgactgtt ttgccgaagc
     2041 aattcaatcc ctcgattggt tgcttcaaaa cctgcgttgt ctccgtcata gcaaaagacc
     2101 aattctttcg tcagcttttc catttgttgg atctgttgag ctgttagact tgtccccata
     2161 gaagctaatc cagttttgat accagcctgc catgcagcaa tcacatccat aaacccttcg
     2221 aacaacaaaa tttctccgtt tttccggata tcgcttcttg ctttatcaag attaaataaa
     2281 acttgtcttt tattgaacag ttctgtttct ggactattca agtatttggg ctgatctttt
     2341 tcctgccctt tttcaggtgc aagccagcgt cctgaaaaac cgatcgtctg gccttgagaa
     2401 ttacgaagtg gaaacatcac tctttgataa aaacgatcag acatacggcc atcatcatgc
     2461 gtgacaaaca atccagagcg ttctaacaat tcatttgaaa ttgattcatt cttgaatact
     2521 tgctgaagaa aagaacgctc attaggtgca aaacctatgt tgaattcttc aattaattct
     2581 aaagacaacc cacgactttg caaatattct aaagctggct gtccagcttt tgtatgcaat
     2641 agcatgtgat gatagatttc cgctgctttt tcatgagcag caatcagttt ccccgtagca
     2701 gattcaggat tttggacagc tatagtacgc tgacgccatt gatcttctaa cggtatctgt
     2761 tcgaagtctg ccacttttac cactgcttct ggaaaactta gaccttctag ttcttgaaga
     2821 aaagtaaaca cgtttccgcc tttaccacaa ccaaagcaat gaaaaatctg cttgtcttct
     2881 gcaacagaaa aagacggtgt acgttcttca tgaaaaggac acagccccaa gtaattttta
     2941 ccagactttt tcaactggac atattgtcca atcacctcaa cgatattcgt ttgactgcgt
     3001 actgtttcga taacctcttg aggaatccgt tgagccattt tttcaccccc tgctctattg
     3061 atatcttttg ggtttaacag aagatatttc aggagaataa ccggtaaaaa atcgcactaa
     3121 ctgaaatcag tgcgatttta aaggacacaa ttatacatgt ttgattgtat catatttcat
     3181 tgctttttca agtataacac atttctatct cattaagcat caatttgcta ctcttttcag
     3241 taactgattt tgaatatcag ccttccagta caatactcga agaacaatat ttacgagcat
     3301 gaatacaaag aagtatccta cgctctctcc caaccctaag tttacttcca aaattccatg
     3361 gaacagcatt gcagctacat atatccctac gatcgtcgtt actgctagga agatgcgcaa
     3421 tgggttgaag ggataacaag cttttacaac tgccatgcaa ctgatcccaa tcagcaagta
     3481 atacatcaaa gtagtagttt cagcatttcc ccatgcgaat gtttttccaa gtagatacac
     3541 cgcaatgata ttcactacta caagcaaggc atttggtaaa gcattaataa gtgctgtagg
     3601 caaataacgg taagtgattt ttcttttatc ttgttcaaaa gataaaaaga aggaaggata
     3661 cccttcaatc gctaaatcaa tcagcgtgat ttggataggg ataaacggaa atccaattgc
     3721 tgtaacggca catataatcg ataaaataaa tgaataaatc gttttgataa aaaagatgcc
     3781 tgagactttc gtcacattgt tgaccactcg gcgtccttca aacaggactt ctggcaatgt
     3841 agtaaaatca gagtccaaca ataccagatt cgcaatctga cgtgttgctc catcaccttc
     3901 tgccatagca atgctgcaat ctgcttctct taacgccaaa acatcattca caccgtcgcc
     3961 tgtcattgca accgttctgc cgctttcttt caattcattt accagcaatt tcttttgctg
     4021 cggactcact cgtccaaaaa ccgtatagcg atgagcagct tctcgcactt ccatttcttc
     4081 agtaatttga gaaagatcga catacgcatc ataattcggt aatcctgcac gtctagcgat
     4141 attggagaca gtaaaaggat tgtctccaga aatcactttc agatccacac cttcctcatg
     4201 aagataagct agtgtttctt gagcgttttt tcggattgga tcatcgattt caaacagagc
     4261 aaccggctca acaaatgctg gtttttcttc gttcaattgc tgatttggag cgatccccaa
     4321 cataagtacg cgatagccat tcgcttgggc tgtttttaga ctgtccggaa gccgatcttc
     4381 ttccaccaaa cgttcaggcg ctcctacgta cacgactccc agttctggaa attccatcgc
     4441 gccccatttg cgatcagaag aaaatgcgac tttgtttgtc acatcatacc gattagaaag
     4501 ctcaaaataa tctctcatag cccgcatagt gatgttgtta tcactgcttt ctgccagata
     4561 cgtccccata atttctggga tggctttctc gtacagcgga tgtagtgctt ctacccgctg
     4621 gatactcatt ttcccttcag taatggtccc tgttttatcc aagcataatg tatcgacatg
     4681 ggccagcgtt tcaattgaat acatgtcttg aacaaggatt cgtttttttg ccagtttggt
     4741 tacccctgtc gttaatgcaa tacttattag caataccaaa ccctttggaa gcatgcctaa
     4801 caaagctgct gcagatgcaa taacagaaat tttgatagaa gcatcgcgga tgaacagtgc
     4861 ttccaaaaat agtatgatcc ccaaaggaat aatcacgaaa ctagtgaatt tggatacttt
     4921 gcgaatcgaa tctacaagtt cagaacgaat agctttgtga gtctttgcct cgctagtcaa
     4981 gcggacagca taattatctg cacctactcg gataacttca gccatgacag aaccgcttgt
     5041 caaaaagctt ccagaaagca attcagctcc agtttctttg acaatcaaat cagattcgcc
     5101 tgtcaaaaga gcttcatttg cttctgcccg tccagatata acctgtaaat cggctggtat
     5161 ctgttctcct gctccgattt ttaccaaatc tcctaatacc agctctttag tagaaatcga
     5221 ttgttcttta ccgtcccgga tcactagtac ttctgattgg gaaacaatcg ataatttccg
     5281 tacaagattt cttgcatgta tttcttgata aattccaata acgatattga cgacaataat
     5341 tgctaaatac gccatgttgg aatacgcacc tacaaataac agacaaatcc caataccaaa
     5401 gttcagaaaa ttgaataacg tcaatatatt atcgctgaat atttgttttg tactttttga
     5461 tgcatcttcc tggtagtcat tttgctggcc agaagccatt ttctgaacta cctcttgctg
     5521 tgtaagtcca ttcattttct ttttctccat tttccgccaa ctttcaaaaa taatcacgtg
     5581 tttaagtcta gcgtgttcca agattaaaaa caacttgttt ttataaaatt tatagaaaaa
     5641 cacacgaatg attttacaaa aaagatgcca acctttttgt ttgaatcacc cgtgtgctgt
     5701 ttttattatc aatctatctt tacttatatt gttcctccaa acggctcatc caccaaccta
     5761 gtgctgtacc ggctataaaa agaacaggac aagctagtac cccagcaact cccaatcccg
     5821 cgtaatcggt aggaatgatc ataatcgttg aagcaaaaac tacacctaaa atgaaatgga
     5881 atagcttgct atatgccttt gagaaaatgt aatccatgat ttttgacaat ccaagaacac
     5941 aaagaatccc accaattccg atcggcacaa tcacttcaag tcgtacttct ttaaatccgt
     6001 cagccatcgc tttatacaat cccatataca ccaaaaaatt agaagggctc aatcctggga
     6061 cgatcatacc caaaccaatc aatccgccgg caatcagcca tgttccaaaa ttctgatcga
     6121 cttgcccgtc aaataattga gcgccgtata ataaaaacaa ataagccaga acaaaagtaa
     6181 caaccatgat gatcacatca gaagtatctc ttcctttttt ccctgcttct ttccataacg
     6241 caggaacagt tccagcgata catccgacaa aaaaccataa tatcgttgtt tcatacgcac
     6301 ctaataaaaa actgactaaa aaagaaagta gaaagatgcc tgtcactccg cctagaccaa
     6361 caggcagaaa aaacaaaaca ttttctttga aattttttgt aaggtgggct aaaaaagaaa
     6421 tcagccgctc atacatgcca aagacagctg ctaatgcacc accgctgact cctggcaaga
     6481 taaatcctga accgataaac attcccttca caaaccgtaa catccagtct ttggttgtgt
     6541 accccacttc ttgattttcc attttttgcc ttttttccac tatttgcgca ctccttcttt
     6601 catttaattt tattcatgat tgaacatttc ctgctatttt acttaaagac tttttcatag
     6661 gtctctatta ttttttcatt cattttgaca atatcaggat aaaacatgtt acctccatta
     6721 tgatagagat agccgccatt gtaaagcaag gactggatcc gccaatatcg gtattgtttc
     6781 ccttcctcat ttcctaaaag tgggctcagc acatctttag aatacgcttc tgctaattct
     6841 actgtgtttt ctccgccatg attttttaca taggcaatat aatctttccc aaaattatat
     6901 gcttggacag ctgtccataa atcgcatcct tgtttctttg cttctactat catttccgcc
     6961 aaatatttga caccttgatc aatactgtca gctggattat caatgatctc agcttctcca
     7021 tgcagacttt ccgagctctg catgagatct gtcccttttc cttttgattc ggtcaatatt
     7081 atcgccttga tcaaattctc gtagttttcc atatgataat tttttgcagc tgttgttatt
     7141 tctatttgat agctttccac tcgttttacc gaacgataag ttcttcctcc taaaaacaac
     7201 accgcaccaa taaccgtcag aatgatcatg taaaaaagaa tacggataat agataatcgt
     7261 tttcgcttca tttcatcacc tagttatttt gacatctttt tttatcttac tatttcttta
     7321 tcttcttggc tatttcttcc ttgatttctg tgtacgcctt aagaaaaaat gaattttcct
     7381 gtattttttg acatcttacg aaaatgatcg ttgtttttca gtaaaaacaa ttgcccaatg
     7441 actgatataa tcgttatact ttacacagta ggtgattatg tgaaaaaaga aaagaaatat
     7501 gcaaaaatgc cagatgggag cgaaatttac tatgaaaaga gcggtcaagg cttccctctc
     7561 tttttactgc atggcaatga cggcagcggc cgcttttttt cagaacaggt ccctgtactc
     7621 gaacgctact ataccgtgta tctagtagat agccgaggac atggtcgttc gacaaacgaa
     7681 gcatctatgt tgaattttca gttaatggcc gaggatctga atacgatcat gttgctggaa
     7741 aaaatcgatc aggcagattt tcttggattt agtgatggag caaatctagc actggtattt
     7801 gccagcagct tccctaaaaa agttcatcga ttgattttaa attctggaaa cacgcttgtt
     7861 aaaggcgttc gtttttctgc cagagtcatc agtaatattc attatgcatg ggtatggtta
     7921 ctctctttat ttcgcccaag tcttcgcaaa aatcttttag ttatcaaact tttgctgcat
     7981 gacatcgggc ttacagaaaa tgatttaaaa aaaatcaact cccccacttt gatcatcgtg
     8041 ggaaaaaaag atgtaatcaa gctaaaacat tctttgtata tagctaaaac tataccaaaa
     8101 gcttcctttg tgcttgtgaa agaacaagga catgaattag ctagaaaaga tcctgaacgc
     8161 ttcaatcgag aagttcttca atttctttct gaaacataac tattaggaat agaaggtgat
     8221 ttatttgtta aaaaatacca tacaatggct gaaaaaccac ctaggactgt tcaaaacaat
     8281 ctttttgatt tctgtcattg tcattattgt cggtgaattg atgtccatcg gtaaaactct
     8341 atctatccag caattaggag aaacgtttgc cactatacct gtatggaaaa gcggactaat
     8401 gctattgatt ggtcttatct ctgtgttacc tatggttggt tatgatatta ttttgaatcg
     8461 gatgcttgaa caaaagcaga atcctcgtta cctgtttgag acaagctggc tgatcaatac
     8521 gatcaacaat attgccggtt tcggcgggtt tgttagtgtg gggcttcgct ctgaactata
     8581 tggacaaaag aaagagagca gaaacgtgat ccaagcatta tcgaaagttt tcttgttttt
     8641 gatggctgga ctttctattt acagtctgct ttctttttta cttgtagtct tcacgccagt
     8701 tgctcctttt ttgaagcaat attggatatg gttgatcggc ggcggtctat attttcctgt
     8761 cgtcttttta tttactactt tcaaaaagaa aggattcctt ggtggtacct cacttaaaac
     8821 tcaaggacag ctattgctag tttcattgtt ggaatggacc ggtgtcctgt ttagtttcct
     8881 ttctgttggt tatttaatgg aaatccggat tgatctgtgg caaacaattc cattattcat
     8941 tgctgcttca gtgataggaa tcgtgtctat gattccagga gaaattggga gtttcgatgt
     9001 aatgatgatt atcgggttgt ccgctattgg tgtgccaaga gaaatagtcg ttgtgtggat
     9061 cttgctctat cgcctgttct actacatcat tccgtttttg attggaatcg tctttttctt
     9121 caaaaatatc ggttccactt ttgatcagcg ttattctggt atccctaaac aattagcaac
     9181 agaaattgcg cataaaatcg tggttgtctt attatacttt tctggaatca tgctagtact
     9241 ttctgccact atcccgcaag cttttacaga gtttcgctgg cttcatagtt tgaacccatt
     9301 gaaatttcat tttattatcc aatttcctag tatactattg ggatttcttt taatcattat
     9361 gggcagaggg atcgcagcac gtgtgaaacg agcatatttg cctacaatct tcttgattgt
     9421 tctagcttta ctttatgttt tattaagtga ttttagtttc acaccggtgg ttttcttgtc
     9481 tattctgctt ctgatcattc ttgcttctaa aaacgaatta tttcgggaac aattgatcta
     9541 ttcatgggag tggcgtacga tcgatggaat cattattggt gcactgtcct tgctttacat
     9601 cgttattggg gtatacaatc tgccagattt ccctcatcgg cgtcaccatt ttatttcctt
     9661 tttcttattt ccttctgaaa agatttggtt ttcgggattg ttggccatca tcgctgttag
     9721 tttcatgatc gttctatttg ttcatttcct tcaaggagaa aagaaacaga ttggcgaagc
     9781 ctttaatgaa gaaaaggccc tgaagatcct tacaacttat ggaggaaact ccgatagtca
     9841 attgatcttc ttaaaagata agcggatgtt tgcctatgaa aaagacggtg aaccaactgt
     9901 tcttctccaa ttcgcttgct tcaataataa atgtattgta atgggtgatc cttctggtaa
     9961 gaaagaagat tttccagaag caatcgaagc ttttatcgaa gaaacagatc gactatgcta
    10021 tttaccagtt ttctacgaaa caagcgaaga gattgttatg attttgcatg aattcggcta
    10081 cgatttcatt aagatggggg aagaagctta tgtagacctg aatagtttta caacttcagg
    10141 gaaaaaaatg aagggcactc gtgctgtctt gaatcggatc gaacgagaag gatttacatt
    10201 tgatgtctta caaccaccat tttctactga gcaaatgagt actttcaaaa atatctctga
    10261 taattggttg ggttcacgaa aggaaaaagg gttttcttta ggctttttct ctgaagatta
    10321 tctacaaaga gcgcctattg cagttgtcaa aaatacccaa gaagaaattg tcgcttttgc
    10381 aacgatcatg cctacttaca cgaacaataa agtaggaacg attgatctga tgcgatatga
    10441 ttctaaaaaa gcaccttctg gcagtatgga tttcttgttc ttgcatttgt ttgcctatat
    10501 gaaagaagaa catatccaat ggttcaattt agggatggcc cctctttcaa atgtcggaac
    10561 ttccagaaag agttttttac aggaaaggat tgcatacttg gtttacgagt ttggttctca
    10621 tttttattct ttccatggat taagagaata taaaagcaag tacgctacaa actgggtctc
    10681 acgttatact ctatattcta gagatagttg gattgcatat gttatgattg ctttgctgat
    10741 tattgataat gcgccagttg agcagacaca aggtagaata agtggtttga aaaaatggat
    10801 acgaagaaag tattaaaaac tgcataccga tagatgaaca ctctcatgat gaataaaaga
    10861 gactgaagta aaacgaaagt tcatgcttct acaatctctt cttatacaat tgaagaacaa
    10921 agagcggtcc aaacagaaat cctgtttgga ccgctctttt attttaaaca tttttcatta
    10981 ccaaaatatt cgagtcgcgt ttcttatgct tcgatctctg taacgatacc tgaacctact
    11041 gtccgtccgc cttcacgaat tgagaatgtc gttccgtttt caacagcgat tggatggatc
    11101 aagtcaacat caatcgtcac gttgtcacca ggcatgacca tttctgtatc ttctggcaat
    11161 gtgattgttc ctgtaacatc agttgtgcgg aagtagaact gtggacgata gttgttgaag
    11221 aatggagtat gacgtccacc ttcttctttt gtcaacacgt acacttctgc tttgaatttt
    11281 gtatgtggag taatggaacc tggtttagca agtacttgtc cacgttcgat atcgtcacgt
    11341 tggatccccc gtaacaacac gcctacgtta tccccagctt ccccgtaatc taacgtttta
    11401 cggaacattt ctacacctgt tacaactgct ttttgtgttt caggtttgat ccctacgatc
    11461 tctacctcat caccgacacg aacagcacca cgatcaatac gacctgaggc aacagttcct
    11521 cgacctgtaa ttgagaagac gtcttccact ggcaatagta atggtttatc tgtatcacgt
    11581 tctggtgttg ggatatattc atctactgta tccatcaatt ccataatagc agcttcagca
    11641 tctggatcgc cttgcaatgc ttttaatgcg gaacctttga tcacaggagt gtcatcgcct
    11701 gggaaaccat attcgctcaa taactcgcga acttccattt ctaccaaatc gatcaattct
    11761 tcatcatcga ctaaatcaac tttgttcaag aaaacaatca ggtattttac accaacttgg
    11821 cgtgacaata aaatgtgttc acgtgtttga ggcatcggac cgtcagttgc agaaacaact
    11881 aagattgcac catccatttg tgctgcccct gtaatcatgt ttttcacata gtccgcgtgt
    11941 cctggtgcat caatatgcgc gtagtggcgt ttttctgttt catattctac gtgtgcggta
    12001 ttgatcgtaa tcccacgttc acgttcttct ggagctgcat caatactagc atagtcttga
    12061 ggatttgcta ggcctttttt gcctaatact gtagtgatcg cagcagttaa agtagttttc
    12121 ccatggtcaa cgtgtccaat cgtaccaatg ttaacgtgtg gtttacttct gtcataatgt
    12181 tcttttgcca taatttacaa acctcctaaa taatatagat cactgaaaaa tttcagctct
    12241 caccttttta ttatagtctt tttttgacta aatcaaaaag aatacgtccg acttactttt
    12301 tgtaagattc gaactaagct tattatacgt caatattggt caaagtcaac taagagcact
    12361 cgtctgtctt atacgcgaaa aaagtacctt ttataaataa aaaaatgtgt tttttgagaa
    12421 aaacttcaag atagtcctta actttctttt gaagcaataa gaacaaaatg aggaaaagtt
    12481 taaaattcag ctgttgccaa caactttcaa tagacgaaat cttctatttc gttaataata
    12541 aggaaagagg tgaagtacat gaaaaaatta attggcgtac ttgtcgtttt aggcatattt
    12601 ctcggcggaa gttttgcagc ttatcattat ttttacggcg gcgaagctta ttatacaaaa
    12661 atcacgacga atggcgaaat gtcagctacc caagcagata acggagagaa attcacgact
    12721 tatacctaca agcaagcggc ttataataaa gatggtgaaa aaaaacaggt gacgcttcgc
    12781 gaagaaagag aacatccatt aaagatgaat gcgtatctta aactcaaagt caatcctcgt
    12841 aaaggtgtat taagctggga agaaataaag gcttctgaag tgcctaaaaa tgcggcagaa
    12901 aaaatcgata attagtaaat ggtcttaaaa ttttattaga taaaaagagg ctgggacaga
    12961 agcgtttaac tccaagaaat aagaaggagt tgcttctgct tccgccgttt atacgtgttt
    13021 agagcgtgag acaaaagtgt tctttacttt tgtctcacgc tctttttttg ttatattcgt
    13081 cttttccgat ccttctcttg acacaattca tttaactgat cactggtact tgaacaccta
    13141 atattctagt catttttata aacgattcaa tacttcatcc acatttttga tcatcacgga
    13201 tatcgtacca tccggattat tcacaaattc aatcaaatct gggttgcgat atacatctac
    13261 tggtacgatc agttcgatcc cgtttgatag cttgaatttt tgttttccaa attttttctc
    13321 agaaatttct tgtacttctt ttacaggagg tgcttctggg acaaatcctt gctcttgtac
    13381 ttcttcttta aaagccattt ttgccgtgat gttttccttg aagacttttt ccgcaatcat
    13441 ctcatgatcc aaccgacctt tttcttctat cgtatcatag acagcttctt tcaaatcagc
    13501 catgatatca aattcttctg tctcaaattt ttttccaaga tgcttggcta ctttttggat
    13561 cacttttaca ttttcttcta aagaaggtgc aggaacactt tcgatcactc ttccagaaaa
    13621 ataaagctct tttttgccag aaaactcata ttttttttca attaattcgt aggtccaatc
    13681 agtcaaattc acagcgaatg cttcatctgc tttttgtgtt ttcgccccta gaattgctcg
    13741 attcaaaatc aagcggttgt cgatccctgc ttcgtctgct tcaacaaagt gagtgtaagc
    13801 ctcacgataa ttgactttca ataaagataa gtacatcact gtatctaatt catacaagac
    13861 aacaaataca tctgcactag gtgcttcttc actttcttgg tagatatcga accaccgctg
    13921 aacgattgct tcacttgctt ccacaaaatt ttcttgagcg attttaagtg attcaacgat
    13981 catgctttct tctgctagtt gtccagtttt tgtttgagca gaagagattt tttggatctt
    14041 cttttgaaga aaatcacgaa tatattcttt tgtcagatcc agctcttttt gtgaaaagac
    14101 tggagaacca gtttctctgt caataatatg cagaattgct ttttttaaat agatatccat
    14161 tttctcattc ccttctgtct tagtttacgg aaaaaaaatc aaaaatacca gaatcaaaat
    14221 cattttcttt taaacaaaaa tttcaagagt agaaacaaaa tggattcttt ttttcgaaaa
    14281 atgcgctaca cttatagttg aacgaataaa aaaggagctg aaagtatgaa ttcattaaca
    14341 agcacttatc gtctatcaaa tggttacgaa atcccggtag taggattcgg cacatggcaa
    14401 acacctgatg gggatgtcgc tgtttcctct gttaaagaag cgctggctgc aggttatcgc
    14461 catatcgata ctgcccaagg atacaagaat gaagaaagcg tcggccaagc gattaaggaa
    14521 agtggaattc ctagagaaga gatattcttg acaacaaaat tatggaatgc aaaccacagc
    14581 tatgaacttg tgatgtcttc ttttgaagaa tcattgaaga agctccaaac agattattta
    14641 gatctgtttt tgatccactg gccaaaccct gttgcttttc gtgataattg ggaacaagca
    14701 aatgcagata catggcgagc atttgaagaa ctgtatgaag caggtaaaat caaagcaatc
    14761 ggtgtgagca atttcttacc ccaccatcta gatacactag caaaaacagc aaaaattatg
    14821 ccgatggtca atcaagtttt ccttgcacca ggcgaattac agacaactgt agttgagtat
    14881 gcaaaaaaac atgatatgat cctcgaagca tatagtccat taggcactgg taaaatcttc
    14941 gatgtgcctg aaatgaaaca aatcgctgaa gcacatgaca agacgattgc acaagttgct
    15001 ttgcgttggt cgctgcaaca cgaattcctt cctcttccta agtcagtgac accaagcaga
    15061 atcaaagaaa atactgaatt atttgatttt gaattaacag aagaagaaat gaaacaaatc
    15121 gatcagttag atggtgtagt cggcaaagca aaagacccag atactaccca attttaagtc
    15181 gaaaggtgat tttatggatc caatcgaacg cttgaattct ctctcggaag aagttaccca
    15241 gacttttcat tccgattttg tctttttgat cgatgcagag aaaatccaac attttcctgc
    15301 aagaaactgg acacatgatc aaatcatcga agaactgaaa aaacggttcg atcattcgct
    15361 aatggtcaaa ccctggcacg aacatgaagt catttattca cctgaactac ctgtttttgc
    15421 cttgataccg aaaaaataga aa
//
LOCUS       DACDIH010000063        14775 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDIH010000063 DACDIH010000000
VERSION     DACDIH010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.063"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            5..1231
                     /locus_tag="IV281_002084"
     CDS             5..1231
                     /locus_tag="IV281_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8781063.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFKEITGISSEKFQKTFPTRQLAIKA
                     ENEMKKKIAKVLREENANSLELKGQIKFKEFYKSKWLPRYELGQTTRSHKAPSDITII
                     NTKNLFRLHILPIFGEYAMSYLNANTEIISDELTKKSKEYANIKIIKCYVRSMFDIAE
                     VLNYIEFNRTTKIIQSITAPKKVALEEKRIREGKQALSSKELTDWLDAVRDDFNDQLL
                     SFHDYTLFMLTLYLGDRKSETYALQWKYIDFENQTVRLKYALDKHTKKKFTKGRKDTI
                     IQVPEIVMTLLQEWKSVQAEQLLKLKIKQTPDQYLFSYSKPSGEVNCPVHTDYLNYRI
                     NSIKKRHPHLVHLTPHKLRHTYATLARQGGADMNQISNALTHSGISTTKIYVNTPDLV
                     DKAVFEAFQRGLQKSN"
     gene            complement(1389..2006)
                     /locus_tag="IV281_002085"
     CDS             complement(1389..2006)
                     /locus_tag="IV281_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8781064.1"
                     /translation="MAKIGYARVSSLDQNLDRQLELLSECDKIFSDKASGKDTNREGF
                     QEMMKFIREGDIVVVTELKRLGRNNKELTETMNLIQLKKATLEVLNLPALTGITDDNL
                     RRLLNNLIIELYKYQAEEERRYIRETQQQGIALAKAKGKYKGGTLKYRENDPRLQLAF
                     NLFLTGSTDKEVEELTGINRRTFRRYRMKHGITNEVREQKNKSFS"
     gene            2312..2650
                     /locus_tag="IV281_002086"
     CDS             2312..2650
                     /locus_tag="IV281_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8781065.1"
                     /translation="MNGIEDENLRRLINSLVIELYKYQAESERKKIKERQAQGIEIAK
                     KKGKFKGRQLKFKKNDPRLKHAFDLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVD
                     QRKNNEKRDS"
     gene            2742..3483
                     /locus_tag="IV281_002087"
                     /pseudo
     CDS             2742..3483
                     /locus_tag="IV281_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320596.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3524..4686
                     /locus_tag="IV281_002088"
                     /pseudo
     CDS             3524..4686
                     /locus_tag="IV281_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(4716..5474)
                     /locus_tag="IV281_002089"
     CDS             complement(4716..5474)
                     /locus_tag="IV281_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8781066.1"
                     /translation="MTYKNIVFDLDDTLYDHQLPFKRSVEKCFPTIDIQQIDNIYKRF
                     RCWSDVAFPKYTKKLISIEQLRIFRCQKTMEEFGIDSISRTEALDFQADYEYELDQIT
                     MIPEIHQLLLALHKNRIPIGILTNGPVDLQSRKLQNIGAYQYFEKQNIIISQSTHGGA
                     AENSYEGNVKNLQSYQNVQLSKRAIEILKEEIELNHQHIRFNPDYKDNGWIFTSKSRH
                     KPGMATVFLTNFIRCRTSFRMLFPIVLDNEPPRM"
     gene            complement(5479..6246)
                     /locus_tag="IV281_002090"
     CDS             complement(5479..6246)
                     /locus_tag="IV281_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8781067.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6735..7160
                     /gene="lacA"
                     /locus_tag="IV281_002091"
     CDS             6735..7160
                     /gene="lacA"
                     /locus_tag="IV281_002091"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP8781068.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            7177..7692
                     /gene="lacB"
                     /locus_tag="IV281_002092"
     CDS             7177..7692
                     /gene="lacB"
                     /locus_tag="IV281_002092"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP8781069.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7703..8635
                     /gene="lacC"
                     /locus_tag="IV281_002093"
     CDS             7703..8635
                     /gene="lacC"
                     /locus_tag="IV281_002093"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP8781070.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8638..9618
                     /gene="lacD"
                     /locus_tag="IV281_002094"
     CDS             8638..9618
                     /gene="lacD"
                     /locus_tag="IV281_002094"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8781071.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9646..9963
                     /locus_tag="IV281_002095"
     CDS             9646..9963
                     /locus_tag="IV281_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8781072.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9963..11669
                     /locus_tag="IV281_002096"
     CDS             9963..11669
                     /locus_tag="IV281_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8781073.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11811..13217
                     /gene="lacG"
                     /locus_tag="IV281_002097"
     CDS             11811..13217
                     /gene="lacG"
                     /locus_tag="IV281_002097"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP8781074.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRLKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            13240..13513
                     /locus_tag="IV281_002098"
                     /pseudo
     CDS             13240..13513
                     /locus_tag="IV281_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
     gene            13534..14433
                     /locus_tag="IV281_002099"
     CDS             13534..14433
                     /locus_tag="IV281_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP8781075.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDEDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSSLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4869 a         2504 c         2846 g         4556 t
ORIGIN      
        1 gaagttgcct acgaagcttt caaatggaaa atataaaact aatttgagat atccaaaaaa
       61 atttaaagaa attacaggta tcagttcaga aaaattccaa aaaacatttc caactaggca
      121 attggctatt aaagccgaaa atgaaatgaa aaagaaaatc gcaaaagtct taagagagga
      181 gaatgcaaat tcattagagc tcaaaggtca aataaaattc aaggagtttt ataaatctaa
      241 atggcttcct agatatgagt tagggcaaac tactagatcc cataaagctc caagcgatat
      301 aaccatcatt aatacaaaga atttatttcg attgcatatt ctgcctatat ttggtgagta
      361 tgccatgagc tatcttaatg caaataccga aataatatca gatgaactca caaaaaagtc
      421 gaaggaatat gcaaacataa aaatcattaa atgctatgtt cgttccatgt ttgatattgc
      481 agaggttcta aattatattg aatttaatcg tacaacaaaa ataattcaaa gtattactgc
      541 acctaagaaa gttgctctgg aagaaaaaag aatacgagaa ggaaaacaag ctttatcttc
      601 gaaagaactc actgattggt tagacgctgt aagagatgat ttcaatgatc aattactttc
      661 ctttcacgat tacactcttt ttatgcttac tttatatctt ggggatagaa agagtgagac
      721 ctatgctttg caatggaaat acattgattt tgaaaaccaa acagtccgtc ttaaatatgc
      781 cttggataaa cacacaaaaa agaaatttac aaaaggtcga aaagatacta ttatccaagt
      841 tccggaaatt gtaatgacac tattgcaaga atggaaatca gtgcaagctg aacaactatt
      901 gaagttgaaa attaaacaga cacctgacca atatcttttt agttactcca aaccttctgg
      961 tgaagtaaat tgtccagtcc atactgatta cttaaattat cgaattaatt ctataaaaaa
     1021 aagacatccc catttggttc atttaactcc tcataaactt cgacatactt acgcaacttt
     1081 agctagacaa ggtggggctg atatgaatca aatttctaac gctttgacac attcaggtat
     1141 ttcaactaca aaaatttatg tgaatacacc tgatttagtt gataaagctg tgttcgaagc
     1201 gtttcagcga ggattacaaa aatctaatta atgtgttcta gaatgtgttc taaatataaa
     1261 aaaaagacac tttccaaata attggaaagc gtcttgaacg ttgatatata aaggatatta
     1321 acgttttgag aattgagatg ctttacgagc tttcttaaga cctggtttct tgcaaaatta
     1381 tattagcctc aactgaaaga tttatttttt tgctccctaa cttcattagt aataccatgt
     1441 ttcattcgat atcttctaaa cgttctacga ttaatccctg ttagctcttc aacttcttta
     1501 tctgtagatc cagtcaaaaa caaattaaat gctaattgaa gtcgcggatc attctcacga
     1561 tattttagcg tacctccctt gtatttccct tttgctttag cgagtgcaat tccttgttgt
     1621 tgcgtttcgc gaatataccg acgttcttct tcggcctggt acttgtatag ttcgataatt
     1681 aagttattaa gtaatcgacg taaattatca tcagtgatac ctgttaacgc tggcaaattc
     1741 aaaacttcca gtgtagcttt cttcagctga attaaattca tcgtttctgt tagctcttta
     1801 ttatttcgtc caagtcgttt taattctgtc acaacgacaa tatcaccttc acgtatgaat
     1861 ttcatcattt cttgaaatcc ttctcgattg gtatctttcc cacttgcttt atcactaaaa
     1921 attttatcac actctgataa taattctaat tgtcggtcta aattttgatc taagctgctt
     1981 actcgagcat acccaatttt cgccataata aaactccttt aaaattctga aatccggatc
     2041 atttttaaat tctgttgccc aactctgttg tcatttttct agataaatgg cataaatttt
     2101 cagcgctaag tgacaaaagc aactaatttt tcatgataga tttttaaata tctcaatcta
     2161 tctccaaaat tagttgcttt tgtagctata gttgactatg aatatgacaa ataattagaa
     2221 aaaactgtgc caattaaata tcaaaatgac aaattgatga atcaaattca aattaagggg
     2281 ggcaaccctg gaagtcttaa atttaccctc aatgaatggt attgaagatg aaaatttaag
     2341 gcgtttgatt aatagccttg tcattgaatt gtacaagtat caagcagaat cagaacgaaa
     2401 aaaaattaag gaacgtcagg cacaaggaat cgaaattgct aagaaaaaag gcaaattcaa
     2461 aggtcggcaa ctgaaattta aaaaaaatga tccacgttta aagcatgctt tcgatttgtt
     2521 tttgaatggt ttatccgata aagaagttga agaacaaact gggatcaatc gccgaacgtt
     2581 tagaaggtat cgagcaagat ataacgtgac agtagatcaa agaaaaaaca atgaaaagag
     2641 ggatagttaa tgagtacggt tattttagct gaaaaacaag ccaggcatta gcctacgcaa
     2701 gtgctttaaa acaaagcacc aaaaaagacg gttattttga gatcaaagac ccactattta
     2761 cagatgaaac gtttatcacc tttgattttg ggcatttagt ggaattagca gaaccaggtc
     2821 attatgacga aaagtggcaa aattggaaac ttgaatcttt gccgattttt cctgatcgat
     2881 acgattttga agttgcaaaa gataagggaa agcagtttaa aattgttgca gaacttctca
     2941 aaaaggcaaa tacaattatt gttgcaacag atagcgacag agagggtgaa aatatcgcct
     3001 ggtcgattat ccataaagca aatgcctttt caaaagataa aacatttaaa agactatgga
     3061 tcaatagctt agaaaaagat gtgatccgaa gcggttttca aaatttgcaa ccaggaatga
     3121 attactatcc cttttatcaa gaagcgcaaa cacgccaaat tgccgattgg ttgatcggca
     3181 tgaacgcaag ccctttgtat acgttaaatt tacaacagaa gggcgtacaa ggtacatttt
     3241 cactaggacg tgttcaaacg cccaccttat accttatttt tcagcgccag gaagctagta
     3301 taaaagtaaa acaagggtca tttaagggcg ttctaagccc cacacagcgt tttaaaaccc
     3361 aagaggagct tttttgcttt tgtttcttct aaacaagcta aaataggcaa tcaagagggg
     3421 ataattgctg atgttcaaac caaagagatg gataggctac aattaactag acagaaaaat
     3481 taaggtgtgt agactagaag aaaatatacc aggaggaatt tttatgtcta agagaacacg
     3541 aagaactttt tcacaagaat tcaagcaaca aatcgtcaat ctttacttag ctggaaagcc
     3601 acgtgtagaa atcattcgag aatatgaact aacggcttca gcatttgaca aatgggtaaa
     3661 gcaatctaaa acgagtggtt cattcaaaga aaaagataat cttacgcctg aacaaaaaga
     3721 attgttagaa ctacgtaaaa gaaaccagca attagaaatg gaaaatgata ttttaaagca
     3781 agcagcgctg atattcggac gaagagacaa gtaatcgatg cgaataagca tctttaccct
     3841 atatcagcga tgtgcagaat attaggtcta tcacgtcagt cctattatta tcaatcaaaa
     3901 ccaaagaaag acgaatcaga acttgaagaa gtagtcgctg aagaatttat ccgcagccga
     3961 aaggcctacg gctcaagaaa aataaaaaaa gccttatcaa aatgaggcat tcagatcagc
     4021 cgacgaaaaa ttagtagaat catgaaaaat agaggattaa aatcgagcta tactgttgct
     4081 tattttaaag tacatcattc tacttgcaat gaagccaaaa cgacaaacgt attgaatcgt
     4141 aaattcttaa gagacaaccc attagaagcg atcgtaacag acttgactta tgtacgagtc
     4201 gggaaaaaat ggaattatgt ctgtttcatt ttggatttgt tcaatcgaga aattctcggc
     4261 tattcttgtg gagaacataa agatgccgtt ctagtaaaaa aagcatttag ccatatcaaa
     4321 caacctctga cagaggttga gatttttcat actgatcgtg gaaaagagtt tgataaccaa
     4381 gctattgatg aattattaac aacttttgac atcaatcgat cattgagtca taaaggctgt
     4441 ccttttgata atgccgtagc tgaatcaact tataagtcgc tgaaagtaga atttgtctat
     4501 caatacacat ttgaaacctt acaacaattg gatttggagt tatttgacta tgtcaattgg
     4561 tggaaccacc ttcggttgca cggtacactt ggctacgaga caccggttgg ttaccgtaac
     4621 cagagattgg cgcagcgaat ccttgataat gagctcggat gtgctaacgc tagcgaggca
     4681 gtctaacttt aactgttagc tcctgccgaa gatcgtcaca tccgaggagg ctcattgtca
     4741 aggacaatcg ggaatagcat acggaaagaa gttctgcacc ttataaaatt tgtcaaaaaa
     4801 actgttgcca ttccaggttt atgacgactt ttggaagtaa aaatccatcc attatcttta
     4861 taatcaggat tgaatcttat atgttggtga tttaactcaa tttcctcttt aagaatttct
     4921 atagcccttt tagataattg gacattttgg taactttgta agttttttac attaccttca
     4981 tagctatttt ctgcagctcc tccgtgagtg gactgactaa tgatgatatt ttgcttttca
     5041 aaatattgat aggcaccaat attttggagt tttcgactct gtaagtcaac agggccatta
     5101 gttagaatac ctattgggat gcggttttta tgtagagcca gcagtagttg atgtatctca
     5161 ggaatcatcg taatctgatc gagttcatat tcatagtcag cttggaagtc tagagcttct
     5221 gtcctagaaa tactatcgat gccaaattct tccatagtct tctgacagcg aaaaattcta
     5281 agctgctcaa tactgatgag ttttttcgtg tatttaggaa aagcaacatc agaccaacat
     5341 ctgaaacgtt tgtaaatgtt atctatttgt tggatatcaa tcgttggaaa acatttctca
     5401 acgctacgtt taaaggggag ttgatggtca taaagagtgt catcaaggtc aaaaacaata
     5461 tttttataag tcataattct agtccgcttt taaaatagta gtgtactggc taaattcctc
     5521 taagtgttga gggctaatct ggttatctgt aatgatggta tcgagttgat ctagattata
     5581 gaagttaaag aaatcgtatc gatcgaattt agtactgtct actaataaga atttttctac
     5641 agcattgttt agggcaagtt gttgaatcac accttccttg tcactatatg tagcaataga
     5701 attatgggta acagcatttg cactaacaaa agctttggca aatctaattg cttttaaatt
     5761 tgtcgaagcc attgaaccta caaaagctcc agttatttct ctatattcac cgccaagaag
     5821 caataaatca atggtttcgc tatcatttag aatcaagaac acagggagac tatttgtaat
     5881 gacacgaatt ttataacctt tacgaccttt caattctaat gccagttgtt caagtgtagt
     5941 tccgggtcca ataaaaatag tatcgccatc tttgattaaa gagcaggctt ttttagcaat
     6001 gtcaattttt tcttttgtat ttagactttt cttctcgata tgtgtctttt caagtggctt
     6061 tttagaggaa agtaattgtg caccaccatg agtacgtgtt aaaagtccat cagcttctag
     6121 agcatcaagg tctctacggg ctgtcatatc ggaaatatct agttcatcta ttatttcttt
     6181 tatggttatg gtcccatcaa tctttaacat atctaaaatt ttttctaatc gtcgtttttt
     6241 gttcatatga agacccttct ttcataaagt aatttttttc caaagataat tctcttttaa
     6301 ttgtatcata aaagacaata ttttcaaggt aaaacaaaca atttcaaaca aaaacaaatg
     6361 ttagatgatg aaataagaac agtggattga cgtatattag cttaggtcag attttgtata
     6421 agacgaaaat aaagtaggac ctcttaatca gtaagttata gaaagtaaaa gacttttgta
     6481 atacttgaat agatatttca cgtccatttt gtgatggatt aaatgaacaa aaatgaacaa
     6541 taatttaacg gtgttatcta ttttttaaga aaacaaataa aaaaaacaaa aaataaacaa
     6601 aaaagtgttg ctttttgttt gaatgattga tattatataa acaaagaaat gatgaaaacg
     6661 ttttttcgaa cattttgcaa aatgttttct acttctacgt agcatttctt tctaaaattt
     6721 aggaggtggt caaaatggct attgttgttg gtgcagatct caaaggtacg agattaaaag
     6781 atgttgtgaa aaattttctt gtagaagaag gttttgaggt aattgatgtg actaaggacg
     6841 gacaagattt tgttgatgtt accttggcag ttgcctctga agtaaataaa gatgagcaaa
     6901 acttaggtat tgtaattgat gcttatggag ctggtccatt catggtagca actaaaatta
     6961 aaggtatggt tgcagcagaa gtttcagatg aacgctcagc ttatatgaca cgtggacata
     7021 acaattcacg aatgattacg gttggcgctg aaatcgtcgg tgatgagctt gctaaaaaca
     7081 tcgctaaagg tttcgtcaat ggtaaatatg atggcggacg tcatcaaatc cgagtagata
     7141 tgcttaacaa aatgtgttaa gaaaaggagt taaggaatga gaatcgcaat tggatgtgac
     7201 cacattgtca cagatgaaaa aatggcagta tcagaattct tgaaatcaaa aggacatgag
     7261 gtccttgatt tcgggacata tgaccacgca cgtactcact accctatcta tggtaaaaaa
     7321 gtcggtgaag cagtagtaag tggtcaagca gacttgggag tatgtatctg tggtacaggt
     7381 gtcggcatta acaatgcagt caataaagtt ccaggtgttc gctcagcgtt ggttcgtgat
     7441 atgacatcag ctatatatgc aaaagaagag ttaaatgcga atgttatcgg tttcggtgga
     7501 aagattactg gtggtcttct tatgaatgac atcattgaag ctttcattga agctgagtac
     7561 aaaccaacag aagaaaataa aaaattgatt gcaaaaatcg aacacgttga aacacataat
     7621 gcacatcaag cagatgaaga tttctttaca gaattccttg aaagatggga ccgtggtgac
     7681 taccacgatt aaggtataac aaatgattct gacagtcaca ctaaatcctt cagtagacat
     7741 ctcttatcct ctagaaacat tgaaaattga tacagtgaat agggtgaaag atgttagtaa
     7801 gacagctggt ggaaaaggct taaatgttac tagagtcttg tatgaatctg gtgataaagt
     7861 tactgctaca ggtttcttag gtggtaaaat tggtgaattt attgaaagcg aattagaaca
     7921 atctcctgtt agtccagctt tttataaaat ctcaggaaac actagaaact gtattgctat
     7981 cttacacgag ggtaaccaaa cagaaatttt ggaacaagga ccaacaattt ctcatgaaga
     8041 agcagaaggc tttcttgacc actatagcaa tcttattaaa caatcagaag ttgtgacaat
     8101 ttcaggaagt ttaccttcag gacttcccaa cgattattat gaaaaactta tccaacttgc
     8161 ttcggatgaa ggagtagcag tcgttttgga ttgttcaggt gcacctcttg aaacagttct
     8221 aaaatcgtcg gctaaaccta ctgccatcaa acctaataat gaggaacttt cacagttgct
     8281 tggaaaagaa gtaacaaaag atattgaaga actcaaagac gttcttaaag agtcactttt
     8341 tagtggtatc gaatggatag tggtctcatt aggtcgaaac ggtgcctttg caaaacatgg
     8401 tgatgttttc tataaggtag atattcctga tatcccagtt gttaatccag tcggatcagg
     8461 ggactcaacg gtagctggta ttgcatcagc tttaaatagt aaaaaaagtg acgctgactt
     8521 attaaaacat gcgatgacat tgggtatgtt aaatgctcaa gaaacaatga cagggcatgt
     8581 gaatatgact aattacgaaa cactaaactc acaaattgga gtaaaagagg tataaaaatg
     8641 gtacttacag aacagaaacg caaatctttg gaaaaaattt cagataaaaa cggtgttatc
     8701 tcagctttgg catttgacca acgtggtgct ttgaaacgtt tgatggcaca gtatcaagat
     8761 acagaaccaa ctgtagctca aatggaagaa ctaaaggttt tggttgctga tgaacttaca
     8821 aaatacgctt cttcaatgtt gcttgaccct gaatatggac ttccagcaac aaaagcattg
     8881 gataaagaag caggtcttct tcttgcctat gaaaaaacag gttatgatac atcaagtaca
     8941 aaacgtttgc ctgactgctt ggatgtttgg tctgcaaaac gtatcaaaga gcaaggcgct
     9001 gatgccgtta agttcttgct ttactatgat gtagacagct cagatgaact taaccaacaa
     9061 aaacaagctt atattgaacg tgttggttct gaatgtgtcg ctgaagatat tcctttcttc
     9121 ttggaaatcc ttgcttatga tgaagaaatc tcagatgcag gttctgttga atatgcaaaa
     9181 gtgaaacctc gcaaagtcat tgaagcgatg aaagttttct ctgatccacg ctttaacatt
     9241 gatgttctta aagtagaagt accagttaac gttaaatatg tcgaaggttt tgctgatggt
     9301 gaagtggttt acagtaaagc tgaagctgct gacttcttta aagcacaaga agaagcgact
     9361 aaccttccat acatctacct aagtgcaggt gtatctgcta aattgttcca agaaacattg
     9421 caatttgctc acgactcagg tgccaagttt aatggtgtgc tttgtggacg tgctacttgg
     9481 gcaggatctg tagaacctta catcaaagaa ggtgaaaaag cagcacgtga atggttgcgt
     9541 actactggat tcgaaaatat tgatgaactt aacaaagttc ttgttaaaac agctagtcca
     9601 tggactgata aagtatagta aaaacataaa acggaggata ttgttatgaa cagagaagag
     9661 atgactctct tagggtttga aattgttgct tatgctggag atgctcgctc taaactttta
     9721 gaagcgctta aagcggctga aaatggtgat ttcgctaagg cagatagtct tgtagtagaa
     9781 gcaggaagct gtattgcaga ggctcacaat tctcagacag ctatgttggc tcgagaagct
     9841 tctggggagg aacttccata cagcgttacc atgatgcatg gtcaggacca cttaatgact
     9901 actgtcttat taaaagatgt gattcatcac ctcatcgaac tttataaaag aggagcaaaa
     9961 taatgcataa actcattgaa cttattgaga aagggaaacc gttctttgag aaaatctctc
    10021 gaaacatcta tcttcgtgct attcgtgatg gatttatcgc tggtatgcca gtcatccttt
    10081 tctcatctat ctttatcctt attgcctatg tcccaaatgc ttggggattc cactggtcaa
    10141 aagacattga gaccttcttg atgactcctt atagctattc gatgggtatc ctagcattct
    10201 ttgttggtgg tactaccgct aaagctttga cggactcaat gaaccgtgac ctacctgcga
    10261 ccaatcagat taacttctta tctacgatgc tagcgtctat ggtagggttc cttttgatgg
    10321 cagctgagcc tgcaaaagaa ggaggcttct tgacagcctt cacgggaaca aaaggtcttt
    10381 tgacagcatt tattgcagcc tttgtgacag ttaatgttta caaggtctgt gtgaaaaata
    10441 atgttactat tcgtatgcct gaagaggttc caccaaatat ctctcaagta tttaaagact
    10501 tgattccatt cactgtagcg gttgttctcc tttatggatt tgaactcatt gttaagggaa
    10561 ctcttggtgt aaccgttgca gaatcaatcg gtacccttct tgctcctctc ttctcagctg
    10621 cagatggtta ccttggaatt acacttatct ttggtgctta tgccttcttc tggtttgttg
    10681 gtatccacgg tccttcaatt gtcgaaccag caatcgctgc tatcacttat gccaatatcg
    10741 atgccaactt gcatcttatc caagctggac aacatgcaga taaagttatc acttctggta
    10801 ctcaaatgtt tattgttacc atgggtggaa caggagctac attgattgtt ccattcttgt
    10861 tcatgtggat ttgtaaatca gaacgtaacc gtgccatcgg acgtgcctca gttgttccaa
    10921 cattctttgg ggttaatgag ccaatcttat ttggtgcacc aatcgtattg aatccgattt
    10981 tctttgtacc gttcattttc gctccgattg tcaacgtttg gatctttaaa ttctttgttg
    11041 acacattgaa catgaactca ttctctgcca accttccatg ggttactcct ggtccgttgg
    11101 gaattgtact cggtactaac ttccaagttc tatcatttat cttagccgga ctcttggtag
    11161 ttgttgatac tatcatttat tacccatttg ttaaggtata cgatgaacaa attcttgaag
    11221 aagaacgttc tggtaaaaca aacgatgctc ttaaagaaaa agtagctgca aacttcaata
    11281 ctgctaaagc agatgccgtt cttggaaaag caggcgttgc taaagaagat gtagcagcaa
    11341 acaataatat cacaaaagaa actaatgtcc ttgttctttg tgcaggtgga ggtacaagtg
    11401 gcttgcttgc caatgccttg aacaaagcag cagcagaata caatgttcca gtcaaagcgg
    11461 cagcgggtag ttacggtgct caccgtgaaa tgttgccaga gtttgacttg gttatcttgg
    11521 ctccgcaggt cgcttcaaac ttcgatgata tgaaggctga aacggataaa ttgggcatta
    11581 aacttgcaaa aactgaagga gctcaatata tcaaattgac acgtgatgga caaggtgctc
    11641 ttgcatttgt tcaacaacag tttgattaat aaatttgtaa aagttatttg agacgagatt
    11701 attctttcag ttccttataa tattgttaac catatcattg catttcattt ccttggtttc
    11761 cggaactcag aatatcgtct caaatgcttt ttttgaaagg atttatagtt atgactaaaa
    11821 cacttcctaa agattttatt tttggtggtg ctacagctgc ttatcaagca gaaggtgcga
    11881 ctcacacgga tggtaaaggc ccagttgcat gggataagta tcttgaagac aattattggt
    11941 acacagcaga gcctgcaagt gatttttacc ataaatatcc tgttgattta gaattagctg
    12001 aaaaatatgg cgttaatggt attcgtattt caattgcctg gtctcgtatt ttcccaactg
    12061 gctatggcga agtaaatgaa aaaggtgttg aattctacca taatcttttt gccgagtgcc
    12121 acaaacgtca tgttgagcca ttcgtgactt tgcaccactt tgacacacca gaagttcttc
    12181 actcagatgg agatttctta aaccgcgaaa atatagaaca ctttgtagat tatgccgctt
    12241 tctgttttga agagttccca gaagtaaact actggacaac tttcaatgaa attggcccaa
    12301 ttggagatgg tcaatacttg gttggtaaat tccctccagg cattcaatat gatcttgcca
    12361 aagtcttcca atcacaccac aatatgatgg tttctcatgc acgtgctgtg aaattgtata
    12421 aagataaagg ttataaaggc gaaattggtg tagtacatgc gttgccaacc aaataccctt
    12481 atgatccaga aaatccagca gatgttcgtg ccgctgagtt agaagatatc atccataaca
    12541 aatttatctt ggatgcaacc taccttggac actattcaga taaaacgatg gaaggggtca
    12601 accacatcct agctgaaaat ggtggagaac ttgatcttcg tgatgaagac ttccaagcac
    12661 ttgaagcagc taaagatttg aacgatttcc ttggtatcaa ctactacatg agtgattgga
    12721 tgcaagcctt tgatggtgag actgaaatca ttcacaatgg taagggtgaa aaaggaagtt
    12781 ctaagtacca gattaaaggt gttggtcgcc gaatcgcacc agattatgtg cctagaacgg
    12841 attgggactg gattatttat cctgaaggct tgtatgacca aatcatgcga ttgaaaaatg
    12901 attatccaaa ctacaagaag atttacatca ctgaaaacgg tcttggatac aaagatgagt
    12961 ttgtagataa taccgtttat gatgatggtc gtattgatta cgtgaagaaa cacttggaag
    13021 ttttatcaga cgcaattgcg gatggtgcaa atgttaaagg ttacttcatc tggtcactta
    13081 tggatgtctt ctcatggtca aacggatacg aaaaacgtta tggactattc tatgtagact
    13141 ttgacacgca agaacgctat cctaagaaat cagctcattg gtataagaaa gtagcagaaa
    13201 ctcaagtgat agagtaatat tacttgaaaa tgtaaaaaat tgttatccga tactttagaa
    13261 aaagtacaac acttatttag accaggcttt gaaaaggtat atattgttag tttcgaagat
    13321 tgtcctatga ttcctgagct tgaagctacc ctcgttgcta aaatgtggta aatggtatgt
    13381 tttaactgga aaagagtgga tttgccattc tgatttagag ttatcagcat ttgaatggga
    13441 atttttacaa tcactcgatg tagaaatacg tgagactatt cacttcgagg taaattattt
    13501 gcctttccaa taataaaaag aaaggaatta gttgtgacta tcgaattaaa aaacgagtat
    13561 cttactgttc agtttaaaac acttggtggt caactgactt cgattaaaga cgaggatggg
    13621 atagagtatc tctggcaagc tgatccaaat tattggaatg gacaggcgcc tattctattt
    13681 cctatctgtg gtagtttacg aaatgattgg gctatttata gacctcaaga aagacctttt
    13741 tttacaggtc ttattcgtag acatggtttt gttcgaaaag aagaatttat tctggaagaa
    13801 gtcaatgata atagcgtgac ctttagtatt aaaccaaatg ctgagatgct tgataattac
    13861 ctatatcagt ttgaactgag agttgtttat accttgaatg gtaaatcaat tagaaccgaa
    13921 tttcaggtaa cgaacttgga aatggaaaaa acaatgcctt actttattgg agcccatcca
    13981 gcattcaata gttctttagt agaaggtgaa aactatgagg attattatat tgaatttagt
    14041 gaggttgagt cttgctcgat accaaagagt tttcctgaga caggattatt agatttgcaa
    14101 gatcggacac cttttttgga aaatcaaaaa attctcgatt tggactactc acttttttcc
    14161 catgatgcca ttacacttga ccagctcaag tctcgtagcg taacgcttcg gtcaaggaag
    14221 tctggtaaag gtcttcgagt agattttgat gattttccga acttgattct gtggtctaca
    14281 actaataaaa gtccattcat tgctttggaa ccttggagtg gtctctccac ttcacttgaa
    14341 gaaggtaatt tcttagaaga taaaagacag gtaacgaaaa tttcacctca agaaacttct
    14401 agaaaaatct atgatattac tatccttaat taaaatttgt tattatttga gtcttcaata
    14461 agatttgaat actcttacct tgtgatgata gattggtctt ctccgtgcca gtcttacatc
    14521 gcaaggtttt taagtttttt gtgggtctat gtcaactgta gtgggtgacg aaaagctaat
    14581 atctaaagac agtctggtag taaaaaataa gattatttac tacttcttga tttaaaataa
    14641 tagtggagcc attaatttgt ggcaaattta atttaatatt ttgcctcaaa ttagccacac
    14701 tttttataga actaagagga aaaatgaaat tttcttccta ttaatatagt tgtttgaaat
    14761 cttaaatata gcgtt
//
LOCUS       DACDIH010000064        14187 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDIH010000064 DACDIH010000000
VERSION     DACDIH010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.064"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(271..612)
                     /locus_tag="IV281_002100"
     CDS             complement(271..612)
                     /locus_tag="IV281_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8781076.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(680..2851)
                     /locus_tag="IV281_002101"
     CDS             complement(680..2851)
                     /locus_tag="IV281_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8781077.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            3062..3919
                     /locus_tag="IV281_002102"
     CDS             3062..3919
                     /locus_tag="IV281_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8781078.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(3990..4847)
                     /locus_tag="IV281_002103"
     CDS             complement(3990..4847)
                     /locus_tag="IV281_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8781079.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(4832..7534)
                     /locus_tag="IV281_002104"
     CDS             complement(4832..7534)
                     /locus_tag="IV281_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8781080.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(7550..8836)
                     /locus_tag="IV281_002105"
     CDS             complement(7550..8836)
                     /locus_tag="IV281_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP8781081.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVE"
     gene            8986..10032
                     /locus_tag="IV281_002106"
     CDS             8986..10032
                     /locus_tag="IV281_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8781082.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            10034..12187
                     /locus_tag="IV281_002107"
     CDS             10034..12187
                     /locus_tag="IV281_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8781083.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(12660..14111)
                     /locus_tag="IV281_002108"
     CDS             complement(12660..14111)
                     /locus_tag="IV281_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8781084.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         3898 a         2974 c         2555 g         4760 t
ORIGIN      
        1 aataggagct atgacaagac ttttgtcgta gctcctattt ctgaataaac ggtgttctcc
       61 tgccaactcc tcggtcacaa gtcacaatag tcatcaaaca tgagaagctg ttttttgaaa
      121 ttgttccttg taactcagag ttaaacggta ggctcacgac ctcttggtaa atggtgttca
      181 agagacagcc tatcggcaat aagaagcaat tttgtcatcg ctcaaggccg aacgtctctt
      241 tcacaccctc tgttgtttca taaaatagtt ttattcgctg taaaggtcgc gaactggtgt
      301 catgatgata cggttcaagt cattgatgat tgtactgaag ccttgctctt tttgcatcaa
      361 gtcattgatt acttcttctg attggatttt cacagccatt tcttgtgctt tcattgcatc
      421 ttcgtcgctg aactcttgtc cactcatttg tttttcttgt aattcgattt gcatcgcttg
      481 gaagtctttg aacagtttgt gtgcttcttc gtttgcttgt actttctcgt acgcttcttt
      541 caacgctttg aattctggta attcacggat ctcacgttcg atttgattcg cgctatcgta
      601 gatgtttgac atgttatgcc ctcttttctt ttagtagtta ctctctattg taacatgggg
      661 ttattcgttt tcaaaggggc tactctcctt gatatttccg ccagaggtct ttagccgctt
      721 caccaattcc ctgaacgcct tctttcacca tatcgccgaa gtttttcagc ccttcttttg
      781 cttgttcacc atattgattc aagttatctt gccaatttcc agactctcca ttagaatttc
      841 ctgtgtttcc aacttgatct gcagctacca cttgtcctcc tgtggcatag gcatctgcta
      901 cactaaatcc tgtttttgaa gtatacggaa ggatgccttc tgccgctgat ttgaagactt
      961 tcccgacaac atttccactt gtcccttcca aatagtgcag cttgctggat tcttcgtaac
     1021 ccatccatgt cgaaatcact acgtctggcg tgtaaccgat catccattgg tcatttgctt
     1081 ttgtggcatc gaaattagtt tctgtggttc ctgttttccc tgctatcgta taacctgcag
     1141 gattcgcagc agccgctgtt ccatttgaaa atgtaccaag aagcatgctg gtcatttcat
     1201 cggctgtttc ctttgatatg acctgttttg gttttttgtt tgtgttatcg acgatcaccg
     1261 ctccagtcga atcaacgatt ttcgtgataa gatgcggcgt atacatctga ccatcattgg
     1321 caaatgcact gtaagcaccc gccatgatca atggagatac tcctttttcc agtccaccaa
     1381 gggctaagcc ataatactta tcagattttg ctaaaggcag gccgaattct tctgctttat
     1441 catatccttt ttgtaaaccg atctcatgca aaagccatac agtaggcaaa ttcaaacttt
     1501 gtgcaacagc ttgatacatc ggtacttctc cttggtatgt cccgtcaaaa ttatgcgcat
     1561 cgtaataact ttgcggttcg tctttcaaga tactggaagg cttgtatcca gcttccagtg
     1621 ctggtgtata tacgcttaat ggtttgatcg ttgatcctgg tgaacgacgc atctgtgtag
     1681 caaagctgaa tcctctaaag acatgatctc ctcttcgtcc tactagtgct tgtacaccac
     1741 ctgtattcgg atctaaagct actgaagcag attggaccat ggcaccatct gaagcatttg
     1801 ctgggaataa tgcactgttt tgataagtag cttctaattg cttttgataa tcctgatcta
     1861 aagctgtata gattttatag cctttattca tgacatcttc ttcatcaaga ccatactcgt
     1921 taactgcttc atcgatcact gcatcaaaat aataaggata acgataacca gaatctgaat
     1981 tatcgtatgt gtcattaaga tacgcgctga tatctgtctg gctttctgct tgttcttgtt
     2041 cttttgtgat ttttccgttt tcttccatta cacttagtac tgtgtttttc cgatcattcg
     2101 cattttttgg gtgatcgatc ggattataaa taccaggacc tttcagcatt cctgccaaag
     2161 tagcagcctc agacagcgta acattcgttg catctactcc aaaatacttt ctggaagcgt
     2221 cttggacacc ccataccccg ttgccaaaat aggcattgtt aagatacatg gtcaagattt
     2281 cttctttgct gtacttcttt tcgatctcga ttgccaaaaa taattccttc gcttttcgat
     2341 caaacgtttg gtctaatgtc agatacgcat ttttggcaag ctgctgagtg atcgtacttc
     2401 ctccgccccc tgatgttccg ccagacaaga ccattcggac tgctgcacgg gcaatccctt
     2461 tgatatcaaa cccgtgatgc tcgtaaaaat tcctatcttc tgtcgagatt actgcatctt
     2521 ggatataggg agaaatcgcg tttaattcta catatgttcc tttttgggcg tacagtgtgc
     2581 cagcttcttc gtttttctta tcataaatga ctgtcgattg gcttaatcct gattttaacg
     2641 tttcgacatt tgcttgtttt gctaaaataa agaggtagat acttgtcact aagacacaga
     2701 tcaaaccaat cagcaaaatc aatttgttca catgatattt tttccagatg cgctttcgcc
     2761 atcgatggaa ttgtccgaga tatggtctga tccaacgcca aaaactgatc aaagcagctt
     2821 ttatcttttc taaaatgctt tgaaaatcca tttgcttctt ctcctgtcta tttgattcaa
     2881 tcgtcttatt ttaccatagt gaaagagtgt tttcttcctg cttaagttgt agttcaatat
     2941 gtttttttac gttagtgcaa gtagagtaac ataagaatga acagaaaaat gtaagttttt
     3001 tcttaaatat gttttaaaaa tggtacaatt agtctgcgat tgcactgaaa ggagtacact
     3061 tgtgaaaatc acgatcactt tacctatgaa ccatcctgaa acaacaattc gcgacttgct
     3121 tgaaaaggaa tggttagttc ctagaaaggt tcgacacttt ttgcgtatac gtaaaaacgt
     3181 ttggatcaat caacaacctg ctttgttcca tcatgaagta aagggcaatg accagatcac
     3241 attgttattc gaagaaacag attataatta tcaagaagtt caattaggag atgcgaagca
     3301 catccgtgtt ttatatgaag atgagcattt gattatagtg gataagcctg caggaatgaa
     3361 gactcatcca aatgaaccga cagaaaacag taccttactc aatcatttag ctgcttatct
     3421 cgacactaaa cagcaacgac cttacgttgt ccaccgcttg gacaaagaaa caagcggtgc
     3481 cgttttattt gcaaaaaatc catttgtctt gccgatactt ggaagaatgt tggaacaaaa
     3541 aatgatctat cggagatatc aagccatcgt atggggagaa attgccaaag atatgaccat
     3601 caccaataaa attggacgag atcgccatga tcggagaaaa agggtcgtag atgataaaaa
     3661 tggtcagagc gccttgacac atgtcactgt caacaaagta ctttctgaac aaacacaagt
     3721 ctattgtgtt ttggaaaccg gacgtacaca ccagatcaga gttcatttag ctcataaggg
     3781 acatccgatc gttggagacc ctctctacca aaatagaaca gctgaacgac tgatgctcca
     3841 tgcattagaa ctgcacatga tccatccatt tactcaagaa aaaatagtcg gtaaagcttt
     3901 acctggatta tgggaataga aataaaaaag aacagagtct tatcagcaga taatgataag
     3961 actctgttct ttttgtgtaa tttgtctgct tacttttgat gttcgtgttc tgctaacagc
     4021 aaactaccga taatcccact ttcattcggc agtccccaac gaacgatata ttcacttact
     4081 tgaggtgttt ccatataacc acccatcaat tcaacaaatt gctgacggat tttttgaagg
     4141 agatgatcct gattcattac cccaccgcct aaaataatct tttcaggtgc taacgtcaac
     4201 gtatagttga tcagtgcttg agcaatataa taagcctgaa tagcccaaac aggatgatct
     4261 tgcggcagat cttgcccttt gatccctgtg cgtgcctcaa tagacggtcc tgctgctaat
     4321 ccttctaaac aatctttatg ataaggacaa gtaccttcat aatggtcatc tggatggcgt
     4381 ttcacccaaa tatgtcccat ttcaggatgt gcgatccctg aaaaaatttc ttcattgatc
     4441 acgacacctg caccgattcc tgttcccacg gtaaggtaaa tacaactatt ttttccttgt
     4501 gctgctcctt gcttcaattc cccatatgca gcagcattga catccgttgt ccaagcagca
     4561 ggaatattgt agcgttcctt gattgtgcca agaaaatcga attgtgccca cccttttttg
     4621 ggagttgcca aaacgtatcc gtaattatct tttgttttgt cgataccaat cgggccgaat
     4681 gagccaatcc cgattccctt caattcataa cgatcaaaaa attcaaatac ctgatccaat
     4741 gtttcttctg gtgttgtggt agggatactt aatttttcta aaatttctcc accatctgaa
     4801 accgcacaca caaattttgt tccgcctgct tctattccgc catacattcg ttttcctcct
     4861 cgaatccgat tgtccgaatc tcataaggtt ggattattgg gacaaccgct tgcttcgttg
     4921 gttcttcaag cagattcaat atgacagttt ttccattgtc tttagttatt tccaattttt
     4981 ctaaatgact agacatattg aaacctctta ctactacttt atcactaaac ttgctgcgtt
     5041 tcaaagctgt aattgcaaac gtttcacttt ttatcgttaa atattgttgt tttgaaatca
     5101 atgttccttc atgatgtttg atttgttgag tggaaaaagg aatctgtgct gcgtatgcat
     5161 gcaaataagt agagaatttt tcttctggtc catgtagttc aatgctgtaa ttgaaccgat
     5221 gctcacctag acactgtgct tctggtgttg gaaaatatcc ccaatcgcct agttctccca
     5281 cactgcgaag tagcgtcact gcgatttgtc cgtcttctgt tacttcatat tcattcaaac
     5341 caaagtttcc gactgttaca ccgtactctt catcatgaat attgacaaat gcttgctgat
     5401 gttgcggatt cgtggggttt tcccaacttt tagacacatg gtttgggcgc ttcaccactt
     5461 caaaaatact atctgcttcg tgatgttcca catgtaactt tgtaggaaac aagacacgta
     5521 agcgatgatc tttcatctga ttgtcgagag tcgtttcaaa gtcgattttt ttactatctt
     5581 ttctgattgt catgattgtt tttatttgaa ggattcgttt ttctttggat cgttctgctt
     5641 ttcgataacg aaattccaca accatctgct gttctttttc taaacgctcg tctgcagaaa
     5701 cagggatttc catttcctgt agaatactta tttttgctat ttccggtgta tctactaata
     5761 cttttacttc agagttttct ttgttactgg ataggattgg ctgatcacag aaaggttgtt
     5821 taaagatata ctcatttcct atatcgccga tatcttcaaa aatatgcaga ttattcaact
     5881 ttcgtttgtt tttgcgatct tccattgtaa tggtaccatt tttttcaatc gtcaaatgta
     5941 gagggccgtt ttcgatgatt cgtccactct gatgaatcat tgtttccttt tcttctgctt
     6001 tcgcttcccc ttctacaagc gcgaatgttt cccaagaaaa tgctggcatg ttttctaatg
     6061 gtaaagtcac tttgatcatt cttgccatat atggcacacg gaaacggtct ttcggcaaat
     6121 cataaccaaa ttggacagtt tcttcagata aaatagctgg tatagtggcc ccactttttg
     6181 tttctacatg atacttacgt ttaggcaaat tttctaactc ttgatatagt tgttcaggta
     6241 ttccttcagc gaatcttttt ctttctaatt cgatggttat ttctgcttca ccagttttat
     6301 ccattcctgc agtatttaca atgacaaatg gaaagctgtc ttttgggaat ccggtcgtat
     6361 cgattgctgc ggtcagctgt tctaaggaat cttgtgcaag atactttcca acttcatctg
     6421 ctttttcaaa acgaggtatc atttcccgat ggacagaatc gactgaacaa ccgcaaatac
     6481 tgtcgtgagg atgattttgc attaatgtct tccaagcata atctaattga tcatggggat
     6541 agtttccact aacttcataa gccatcgtcg caagtggttc tgtaatattt tctagctgtc
     6601 gctggacctt ggtattccat tgttttaagt aaacccgagc agatgctgta ttggctaaag
     6661 tataccagcc atccgtttct tgacttgtca attctccttc tactgagcct aagtcttctg
     6721 gcacatcttt ttgaactgct tccagatagt cagtaaaatt agaatgaata aactcgtaat
     6781 ctggaaacag ttcatttgcg agatggattg cttttgatat atctttttgt accggttgat
     6841 gatcaacacc attcatcatc aataagtggt tcgtagaggc atattgttct gcatccgcta
     6901 atttttgctt ccaaaaggca agagctgctt ctttttctac tggaatctca ttcccgttgc
     6961 tataccaatt ggcaaatagc agaccaaaga ttgctgtttg atcaggtcct ttccaccaca
     7021 tctctgagta ttgggaagaa tagttttctg cttccaaaac ttgattatca aagccaattg
     7081 gttttacacc acggccaaac gctgctgctg aaataccagc ttgtttcatt aattgaggcg
     7141 tctgtcccat atttccaaat gtatcgggaa aatagcctaa catgacaggg gttccccatt
     7201 tacggctttc ttccatgcca atcagcatat ttctcacgtt cgattccgag ctgataagaa
     7261 aatcatcttg caagatataa aatggtccaa ttctcaattt tccttcattg attgcttgtt
     7321 ggattgcctg tcttttttct ggccttactt gaagatagtc atctaaaata atcgtttgcc
     7381 catccaaatg aaaactgttg aaggacggat caacttcaaa tagctctagt agatcgtcta
     7441 tcaattcaat cagtcgcatg tgatgctgtt cgtaagccat gtaccattca cgatcccagt
     7501 ggctatgaga aatgatatat actttctttt tcatttacga tacctcctac tattctactc
     7561 taatatcaaa gtaatccatg actaattcgc aaaacatcat atttgcccaa gaaaaccatt
     7621 ctctcgtata attttcagga ttgttcacat cgaatccttc gtgcataagg tgtgttcctg
     7681 cgtcagttgc tacaagtaaa ttcaatatcc gctctttttc agatttgtct gacgttgtca
     7741 ttccttccat agctaatgca atcggccaaa cgtatttttc tggcgtatgg gaggatccaa
     7801 ttccttttgc gtactttcct tcatagaaat atggattttc tttactcaac aatgttcttc
     7861 ttgtttgcag ataatcttcc tcttgttcag aacaatatcc caagtaaggt gcggcgatca
     7921 gattgggcac attactgtca tccatgactg tggcgtttcc cattccatct acttcatatg
     7981 cataaatcgt ttgccctttt tgattaaccg ttcgtccgtg attctgaatc ccctcttgga
     8041 tctctttctt caatttctgt gtttgctgta caatttcttt atcatcgagt atcgtttgaa
     8101 aaatttcttc cacataacct aacacgacca ccgcaaacat attggatggt accagatatc
     8161 cataacggca agtatcatcg ctaggacgga aaccagacca tgtcattcct gtaaaagctg
     8221 tttttgcacc tcgcccgtca ttgatcagcg tatcttcttt tcttgtcgtg tctcgtacaa
     8281 atgtataagg agagtgttca tgatcttgct ctgttttgaa cacgtccaag atttttttga
     8341 taccttcaat aaaagattga ttgaattgat cagtttttcc tgtatttttg taaagaagat
     8401 aggccaactg tattgggtag cataaagaat caatttcata ttttctttcc cagatccaat
     8461 cgttcatttt tgtatgatct gtttggtggc cagcaccgtt agcttcttcg ttgaaggcat
     8521 tcgcatatgg atcgatattg atataaaaaa attgcttttt gactaacccg ctgatcatgt
     8581 cagctaattc ctcatcttct tttgcaatca ctaaataagg acgaacttgt gcagtagagt
     8641 ctcttaacca catcgctgga atgtctcccg tcaataaaaa ggtggttccg tcattttgct
     8701 ttttgactgt tgtcaacaga gtattggcaa atgctgctgt aaaattcttt gcccagtccg
     8761 catggatatc cccacatttt tgtgtgatat cttccataaa tttctgcacc gattttggta
     8821 tatctttata agccactaat aaaaccttct ttctatttga tatatcttta aagattatac
     8881 ttcatacagc caattatgtc tacgctttca aaaaatatct ttttatttac tttttcatcc
     8941 attatggtat tatttgaata tgatatatca ttaagaggtg tttatatgaa tcaaccattg
     9001 taccagacaa tatttaatga tttgcaaacg gctattttaa ctggtgaatt acctgtagac
     9061 agccaactac caactgaaaa agaactttca cgtacctata aagtaagcag aatcacttcc
     9121 aaacgtgcat tgactgaatt agagcaatta ggacttattt atcgcgttcg cgggaaaggc
     9181 agtttcgtta aatccgcttc tccggaacag attcttgctt ctcctacaaa aacacgacgg
     9241 attctgtttc ttcttccata tgtcgctgat ttgtctgttg gtgattttag caagggattg
     9301 aaccctgtga tgcaagataa acagattgat gtcatgatga cgacgcttga ctttctacaa
     9361 aataaaacag caaaagagct aatcaatgaa tttgacggat tgatttatta tgctcttgat
     9421 acagaacaac atcttgactt actttttgag ctttcattga aagaattccc tgtcgtcata
     9481 ttagataaaa aaatctatga gttacctttt cctaccgttc tttccgataa tttccaaggc
     9541 ggtgcgcttg ctgcctctta tttgatcgaa cagggacatt caaaaatcgc ttatttgttt
     9601 ggtacgcaga ctcatccaca atccgttcga cagcgttacc ttggttacct tgaagcattg
     9661 gacaaagctg gtttgacttt ccacacttca ctggatgaca aaagcgcgac attagatgaa
     9721 ttgctttctt atatcactaa gtatgaagta actgcttttg tttgtgaaaa cgatgttgtc
     9781 gctatccaga ccatgaatct cttgagacag catggttatc atatccctga agatttttca
     9841 attatcggat ttgatgatat ccaagcagca gctcttgttg acccgccact gacaaccatc
     9901 gcacaagatt ttgaacagtt aggtaagctt gcgggagatg ctttggtcac ttggttagag
     9961 acaaaagatc gaccatccga tagtaaattt ccagtttcat tcatcaaaag acaatcaaca
    10021 aaggagaaat aaaatgaata tcggtcaaat agatactaga catgcaacag cgaatcagcc
    10081 aaatttctct aatgggaact gccagcctta tactggcgtt ccttttggga tgaattattt
    10141 tgcaccgcaa actacggatc aaaacggcag ctggtggttt catcctgatg accgcgtttt
    10201 tcaaggctac cgtcttaccc atcaaccaag tccttggatg ggtgatttca gtcatatgct
    10261 gtttacgccg atcaatggaa aactacagga gaacaccctt ttccatgctc aaagttctta
    10321 ccgcccagaa gaaagtatat tttgcccgac tcatctgtcg atctgtcaat tacgttatgg
    10381 catctgttct acattgatac ctagcatgta tggcggaatc ttatctattg attacagcaa
    10441 aaatgacagc ggtttacttt tatcctttcc gggacgccat cagctctttg ttatagatcc
    10501 ttacacagtg gaaggaaaaa tcacacattt tgctggttca gaagatcctg atttttcttt
    10561 ttattttata ttgagatttg aatatccttt aacaattgat gagttagctg tttccaacaa
    10621 aaatagcgac tcgatcccgt tgtattttga acagacaaaa aaacaaacta tccgatttgg
    10681 gacatctttt atcagctcgg aacaagcagc ttataacctg gaactagaac aaaaccaaag
    10741 caaagagact taccttgctc acagtcaaga acaatggcag tcctattttg atcgtataca
    10801 gctcacacac catgatcaac aacaattgcg gacgttttat cataatttat atcgggtgtt
    10861 tttatttcca caaacatttt atgaaattga tcctagtggg caaaaaatcc actatgatac
    10921 gactagtcga tctataaaac ctggtgttct ctatacaaat aatgggtttt gggatacgta
    10981 taaaacggtt tatcctcttt attctttgat tgctgtagaa aaatacgaag aaatgctgga
    11041 aggttttttg aacagctata gagaaagtgg ctatttgcca aaatggctgt ctcctgatga
    11101 acgtggactt atgcctggga cattgatcga tgcggtcatt gcagatgctg ctgtaaaaga
    11161 tatccgacga gacttgatgc ctgaatttct aacggcaatg gaaaaaggtg ctacggttca
    11221 aagcaaacat ccaaattatg gacgccaagg aacagaagat tacttgacat atggctatgt
    11281 tcctaatcac taccacgaat cagtcaatca cacattagac tatgcttata gcgatttttg
    11341 tattagccaa gttgccaagg tactagaaga agaggaaatg acgaagaaat atcagcagca
    11401 atcattgaac tatcaaaata tctttgacta tgagtcagga tttatgcgtt caaaagataa
    11461 agatggcaag ttcagagaac cattttcagc taatcgttgg ggacaagatt atgcagaagg
    11521 aagtgcttgg caaagcagct ttgctgttta tcacgatttt tcaggactga tcggaaaata
    11581 tggtggcagt gagagatata aagaaaaact cattgagctg tgcaatcagc ccccaacgtt
    11641 tgacccagga ggatatggtt ttgagatcca cgaaatgagt gagatggccg cgatagaatt
    11701 tggacaatta gctatttcaa accagccaag ttttcactat ccttacttgt tcagttatat
    11761 aggacaacct gaaatggcac agccactgct taaacagttg atgactcaga ccttcgacga
    11821 ttctccaatg ggttaccctg gagatgagga caatggaagt atggccgctt ggtatatctt
    11881 caatagtctg ggattttatc ctgtcacgcc tggaaccggt gaatatgtta tcggtatgcc
    11941 attaattgac aaggctgttt tgcttttgtc aaatggaaag gaattgacga ttgaagcaac
    12001 gcctaatcgg ccgcagcaac aatttatcca taaaatcaaa cggatggaca aagaatatac
    12061 gaaattatat tttacccatc aagatttgct gaaaggcggc acgatctctt accaattagg
    12121 gatcatccct caccctaaga aatatcaagc aaaagatttg cctttttcct tatcaaacta
    12181 taattaacaa gaggttgtga aaaagctgtc ctgcatcaag taataagaac agccaagcaa
    12241 aaatagatcc tcatattttt cgtttggttg gtcttattac cgcagatgca agcttttgaa
    12301 caccgtttat tgagaggttg tgaaatcagc gttctgacct gagtattaga gagacaaatg
    12361 gcaaaatagt tcctcatatt ttgccatttt ttgagctaat accgagggtc agctgattga
    12421 acaccgttta gtggagaggt cgtgaaaaag ctgtttagtt tcgagtatta agaaaaattt
    12481 tgtgaaaatg gctttccaca tttttgcata aatttggctt aataccgaga aactggcttt
    12541 tgaactccgt ttatcgagag gtcatgaaaa agctgccttg cctcaagtaa taagaacagc
    12601 ccaaaattag atttctagac aattttttat aagttatttc attacattgg ctttttcttt
    12661 tatgattctc cactgttttc tcggtactct ttaggggatc tgccgacgat tttcttgaat
    12721 aatttagaga aatacgtggt gttgttgtag ccaatctcac ttgctatctc attgatgttt
    12781 tgattactgt gcaaaagcag ttgtttcgct ttttccatac gtaaatggtt caaataagct
    12841 gaaaaacttt ttttcgtttc ttttttgaac agctgtccta gatacatgac atttaaatgc
    12901 aggttttctg acacttcttt gagtgtaagt ggttcttgat aatgctggtg caggattacg
    12961 attacttttt ccacattgtc actgtacttt ttctgtctct gatgcttttt gatgaatgac
    13021 agaagtagct gctgaagttc ttgcaccgtt tttgcctgat ggattttttc gattccttgt
    13081 aaatattcct catcttccag atcaatcaat tcccgttgaa tctccataaa cagtaaaaaa
    13141 ctggaatatc gtatatcttc cggcatcatg gctgctaact gaaactgttc aaaaaaatca
    13201 gtgatcattt tttgagcgac ttccaattgt ttattttgca gcacgcgtct gaaacttgaa
    13261 aaatcgatgg tttgatcttt tgtttctgct tgtactgcgt aaaaaacatg ctcgtcttta
    13321 tctccataaa attgatgcaa ctgtagattg tctttcgctt gttggaatgt ttcagggatt
    13381 ttgtccaaag aatctgtctc ttccccgata ctgatcagcc attcctgatc aggaaagttt
    13441 tcttgaacaa aacaacagaa gccatcaact tcatctgcct ccaacaacaa cgtgatcaat
    13501 acacggtctc cgtaatttcg ttgatagtaa aagatttgtt gccgttcttg caaccatcta
    13561 ttgataagag tatcatgagt tcttgccagt tgaatcaata ctatcctatg ctgcttctca
    13621 cttatttgtc ctattatttc ttcttcactt atttcatcca attcttcatt caaccattga
    13681 ctgaataaca gttcttgata gatttcttgt tgattttttt gttcattttg ctgatctaac
    13741 cgtgttttga ttttcttgag gctttcaacc aattcgaact tattcacggg tttcatcaaa
    13801 taattcacgg cgcctaattg catacctcct ttcaaataat cgaattcttg gtatcctgat
    13861 aaaatcataa attcaaaatt atgacgttct ttttgagccg cttcaataaa ttccagtcca
    13921 tttatttctg gcattgtcac atccgttact actaaatcaa tctgtcgctg ttcaagtaca
    13981 gctagtcctt ctttggcatt ctctgctgtt gctactattt gaaagcctaa actttgccaa
    14041 tcaattatct tttttaaccc attcaaaatc atgtattcat catctaccaa taacacttga
    14101 tacacgctca tccctccttg aatgtaatag aaacggtcag tccacctgtc tcattattct
    14161 caagcgacat ataataactg ctgccga
//
LOCUS       DACDIH010000065        14176 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDIH010000065 DACDIH010000000
VERSION     DACDIH010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.065"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(637..1302)
                     /locus_tag="IV281_002109"
     CDS             complement(637..1302)
                     /locus_tag="IV281_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8781085.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1428..3464
                     /locus_tag="IV281_002110"
     CDS             1428..3464
                     /locus_tag="IV281_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP8781086.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3468..4106
                     /locus_tag="IV281_002111"
     CDS             3468..4106
                     /locus_tag="IV281_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8781087.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4134..4562
                     /locus_tag="IV281_002112"
     CDS             4134..4562
                     /locus_tag="IV281_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781088.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5019..5930
                     /locus_tag="IV281_002113"
     CDS             5019..5930
                     /locus_tag="IV281_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8781089.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            6147..6584
                     /locus_tag="IV281_002114"
     CDS             6147..6584
                     /locus_tag="IV281_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781090.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            6679..7563
                     /locus_tag="IV281_002115"
     CDS             6679..7563
                     /locus_tag="IV281_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8781091.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            7787..10927
                     /locus_tag="IV281_002116"
     CDS             7787..10927
                     /locus_tag="IV281_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781092.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEIT"
     gene            10974..11519
                     /locus_tag="IV281_002117"
     CDS             10974..11519
                     /locus_tag="IV281_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAP8781093.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            11547..12002
                     /locus_tag="IV281_002118"
     CDS             11547..12002
                     /locus_tag="IV281_002118"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAP8781094.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            11999..12757
                     /locus_tag="IV281_002119"
     CDS             11999..12757
                     /locus_tag="IV281_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8781095.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(12843..13583)
                     /locus_tag="IV281_002120"
     CDS             complement(12843..13583)
                     /locus_tag="IV281_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8781096.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            13584..>14176
                     /locus_tag="IV281_002121"
     CDS             13584..>14176
                     /locus_tag="IV281_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8781097.1"
                     /translation="MQAPIKKICVHFETKQWRNKMVATVTLYLTFTLILDLGIYLFFS
                     KQLLVKNSFGCIALMVVTLLLPIPKVSFPYLIGLLLMFLFYFTTKNLFISCLISNLFF
                     AITGLSFFLVFDLMDYLGKRHLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPT
                     EHYFGGICILLSWGVIYLVLGSVHFRSSNYLLLSLLS"
BASE COUNT         4758 a         2331 c         2820 g         4267 t
ORIGIN      
        1 ttcctactaa tattcccact aacaagagat aaaacggcca ggttggtgtg attcctctat
       61 aaaaaaatgc ccatgctacc gaaatcccat agattaacaa ctgaacaatt tttacaatga
      121 aaaaattgtt ctgccattac tcaaagtctc ttcctccttg tatcgtttat ttgtttcatt
      181 ttatcattat gataggcgct tgaacaatcc attttgtatt ttattgagta ctcttggata
      241 aactaccagc gattatcata tttatttatg ttactgtaag ccatcatata aaagtaaatg
      301 agcagatcat agaaatataa aaaagaacta aaattgatct aaattatgtg cagcttgact
      361 acgtaccaat ctatattact tactagattg gtcgttaatc tattcggatc gaatgagcaa
      421 aatatttttg tgaatgtcca tccttgatta tgattccaaa aagaatcatt ggaaggctgt
      481 ggcgtaaata ttatactata agccagaaat acctatcatt ctgaactttt ttatttagga
      541 actttcatct caaggctttt tcaattaatt tgtacccctg ttttttggat aagatcatgt
      601 gcattgattt tttctatctc tacgttcatc tcctacttaa tgcctatctg gctcctgagg
      661 gcttcttttt aaggcgtcgt catagtcttg gctgaggatt tcttggtaat ctgagggaat
      721 ggcttcccaa gtgagcaggc gaacgttcat gacgtgtttt cctttgtgtt tgattacagc
      781 atggtttttc tctttttcta cctctccagc gtgaaaagac agccatgtac catggccaaa
      841 gatatcatta catacccaac gttcgatgga attgtccacg tatcctacag ggagtgtggt
      901 atagccgatt tgtggtttca aaaaaggatt gaatcgatcg atcgtttcat ttttgtattg
      961 ataattgata agaaaaagat agaccaaaca aaagacgagc agaaagaaag atagtgtctt
     1021 tttgagtgag aagggttgtt ttgatgaata agatttcttg ttaacaagag gttcatctgg
     1081 caatgttggt tctattgatt cggtagctga ttcaatagaa ggaaaatcaa tcggcagacc
     1141 taaagtatga tgtaaaagtg acagcatttc aaagtctggc attgtacgag aattttccca
     1201 actagaaatc gttttatcgg aaacaccaat tttttcgcct aattcttttt gcgtaagctt
     1261 acaatttctg cggtaagttc gtattttttc tccggtgttc atcttcttct cctttcgatt
     1321 ctacagtata tagatttatt ctacatgacc tatacacttg atagatacgt ttttttgatg
     1381 aaaaacggta tgattttatc aaagaatcat ttaagtaagg gagagtggtg ataatcaaaa
     1441 aaaagttcac agttttactc acaatcatag tgaccggtat catgagtggt tttgccttgg
     1501 ttagtataaa taaacaggta ctctatcgga caaatgaatt tttccatcaa acagatactt
     1561 atactccgat tcgattgcca caacatttta ttgggcaaga agaatatgag cagctaatca
     1621 tggtatttga aaatgtctca tcagaaactg agctttctta tatgaaaaaa acagtaaatg
     1681 aatccaatga agtagataca tcttggaact tcaagataca gccaaagctt gaaatgacat
     1741 tgtattatca aggggatgtc aaatcttctc acgagaaggt accctattct aatacctcct
     1801 ttcgcgtaac gtccattgaa gaatcgatca aaataaaaga ttttgaaggt gaatttttct
     1861 tgatgacgag agaaaaagaa gcctatgaac gggtcatttc actattcact gatcgattca
     1921 atcaagcatt tcacactgat tatgccacta atatttttga agactttgaa aatagctatt
     1981 cgtattcggt tatttcaaat acagacgtat ataattatcg agtgtattta actattggat
     2041 tgattctttt attccttttg ttatcatcct ttttcttatt gatgaataaa gaaatcagtt
     2101 tattgttgaa taatgacttt tcattacatc aagtattgtg gtgtttattg gggaaaaaga
     2161 aaatcattat tacattcata attgcaggaa tcattggtgg ttttattttt atgtattata
     2221 aggagcaatt tctttatttg ttccagatgt acgttttctt gttcagcgga atatatggta
     2281 tctctttgat ttctctttta ttagttgaaa aaatcgcttc gttaagagaa gcgttcaact
     2341 atatccaata cattcttttt acatttggat tgttgttttc tcttcttttg accgttgcca
     2401 atgtcgaatt agcaactatc gttttttctt cttcacagtt agctgtttcc catgaaccac
     2461 caaaagaaat cgctggtgag gactatcatg tattttatcc agtaaccatc gggaaaaacc
     2521 acgtggattt tatttattcc aatcgttttg catcagcagc agatcaggaa ttatatgaaa
     2581 ctttgaataa aaacggaagt atattagtca atgtatcaga ttacttgaat gaagaaaatg
     2641 aacagtttgg cagagggata aaaataaatc tgaattatct taagaaattt cctttgattg
     2701 atgtctctgg aaggttgatc caaataaccg aaaaagaaac gcaccctgtt atattgatcc
     2761 ctgaacgata tagacataca gatttaaaaa atgacctggc gcaaataaca gaatattatc
     2821 aagaaatcag tgaaaaagaa ccgaaatttc tatttataaa aaacaatcaa cccatttata
     2881 catttattcc ttctatcccc tggatcgaac attatccggt tgttgaaata ctgacattga
     2941 aaaacagtat gtatttggaa agaaatattc tcagcggaga aatctatcca ccgttaaaga
     3001 tcaagatagg ctctttgtcg aaagaaaggt tatttgagct gattgaagaa agtcagttac
     3061 gtgataactt gcagttatca tttccgtata ctcagactga agagatagct gtcaaagtgt
     3121 taagcagatc atttcattac ttgatacaga tatttttact cactttcttt tctttcttct
     3181 tgttgagcta tgtcatgtta tgcatatatt ttgtctacaa ttgtcggaaa atcgctatct
     3241 ttcgttcaag tggttattcg ctctttgaaa cgtataaaga tttcttcatg atgaacctca
     3301 tcaaatgggg aacgacaagt gtaatcttcc tttttcttat agaaagagag cctaaatatc
     3361 tttttaatat ttttttcttt agcttcattg attttattct ttcagttgtt tttattttgt
     3421 ctacagaaaa gaaaagtcag ttattgctga tgaatggagg ttaacaattg gagccgttga
     3481 tagtgataaa aggtgcgaaa aaaatcattc gaaacaaaac gattcttcat ttggatcgtt
     3541 tcactgcgga tgctggggat cttgtgactg ttgttggaga aagtggagca ggtaaaagta
     3601 cgttattgaa tatattagga ttgaatgatc agttcagttc aggacaattc tatttgttcg
     3661 atcaagagac gaaacaatta actgggagag agaagcgctt gataaaaaga aataccatat
     3721 cttttctttt tcaagatttt gggttgattg aatatgaaac aatcgatttt aatttagata
     3781 ttggattgaa gttttcgaat ttaaatgttt cagataagcg aacagcaaag tatgaggcat
     3841 tgaaaaaagt aaatataaac aagaagctaa gtacgcctat ctacacatta tctggtggtg
     3901 agaagcaaag aatcgcctgt gcgagagtct tattgaagaa tagcaaaatc attttagccg
     3961 atgaaccgac tgggtcattg gataaaagaa ataaggataa agtagttgaa atcttatcgc
     4021 aacaagcttc ccaaggtacg ttagttatcg tagtcacgca tgatccatat ctgaccaaaa
     4081 taggaaacaa gaagatcatg ctttaaaaat caaaaaggaa aggatgtcgt tttatgaaaa
     4141 aagcagttat tatttgtgca ctagggatag gttttgttgg aggaaacgct atgtcatctg
     4201 aagtggaagc aaattcagtt cacggtgaaa tctattacaa atctttaaag ccaggagaga
     4261 ttcattttga tgctaaaggt aatattatta agtcaaattt accagtagac cgggaaattt
     4321 ctagaacagc aattaatcat agctctggaa gatgggttta ttatagtgat acaacttgta
     4381 cgagaaaaac aggtcattct aaccactata gtcgtgtcta taatcgtcac ggtgcaaaag
     4441 cgaaagttgg aaactctatt cgatcagcaa atgcaacaaa aggagtatgg gcctatgcaa
     4501 gtgcgacagg aaatagaact gacacattct catgttggta caatccaacc ggttggtatt
     4561 aagtatccaa aagtttatat aaaatagaaa aaacatataa tgaaacagcg ttaaaagtta
     4621 tttttggtag ttttaatata ttcattttta gttgtataaa aatatatcat aataaaaaca
     4681 acaaaaaaat gtctggaaaa tcgaacataa aagtgtctga tttgaatatc ccagcagcgg
     4741 aagaagaaaa acaaaatcga taacatttgt tctaagaatt agttacgtta aatacggaac
     4801 gcaagataga cttgaaaaac ggtaacaaaa gaagtagtat attaaggtgt gatgtgctac
     4861 ttgacattta ttctgctgag cgaactttct ggattgtgta gccttatatc tattcaggac
     4921 gtatcgagca gattagtaaa ggtgttcttg atgattttca tcttgaacac ctttgttttt
     4981 agcaattcgc aaaaaacgaa aagtaggaga attgataaat gaaaaaaaga tttttagcac
     5041 ttgccatcgt actaggcact ggcttattgt caggctgtac gaatgccggt gaaaaaacag
     5101 ccgttagtta taaaggcggt acaatcagcg aacaagaagt aatggatagc ttgaaaaaaa
     5161 tgcaaggagc agattccgct gtgcaacagt tgatcgttta tcaagtattt gaagataaat
     5221 atggagatga cgtatcaacc aaagaaatcg attctcaata tgatcaaaca aagaaacaat
     5281 tgggtgattc atttgattca caattgaaat ctgcaggtta cacagaacaa acattcaaag
     5341 acagtatcaa acaaagtcta gcctttcaag aaggtctaaa gaaacatatc aaattgaccg
     5401 atgaagattt aaaaacggct tgggaatctt tccatccaga agtggaagct caaattatcc
     5461 aagtagcaag tgaagatgat gcaaaagatg tgaagaaagc tgctgataag ggagacgatt
     5521 tctctaaatt agccaaagac aagtcaactg atactacgac aaaagaagat ggcggaaaag
     5581 tgaaatttga ttccactact acaacggttc cggcagaagt caaagaagca gcctttaagt
     5641 tgaaagatgg tcaagtttct gatgtgatca cttctaccaa tgcttcaact tatactacgg
     5701 aatattatgt ggttaaaatg gtgaaaaacc aaaacaaagg caacgacatg gataaataca
     5761 aaaaagaatt gaaagaaatt gcaacagaca caaaattaag tgacagtacc ttccagaata
     5821 aagtaattgg cgaagtattg aaagacgcta atgtaaaaat caaagacaaa gactttgaaa
     5881 atgttttgag tacattcact tcagattcta gtacagcaag tagtaaataa gcggtataaa
     5941 taaaaaatat gaactattta aatcaataaa actgttcatc taaatctttt tattagaact
     6001 aaatcaacag tagtttaatt actacttgat ttagttcttt ttattttaaa aatttttttt
     6061 acttagaatc cagcgaccct agatattttt ctgtataatc ttaaataaag aaaacaatat
     6121 caaaaaatgc acgtagaagt agataaatgg agtggttttg caaatttctt ttatttctag
     6181 aagttattca cgtatacgga tttgtttttc tcacaaataa aaaggcagga agtacaatgg
     6241 atcatttgta tataaaatat attcatcaat tttccgtttt ggtgttaggc acactaatta
     6301 tagcggggat tgtcgcattt attattcaag gccgtgacat attaacagta catcaagagt
     6361 ttttcagtta tgcatttggt attcctctga tcgctataag gtctgataac aaaaagtgga
     6421 agaaacgcta ttacattctc ttgctatttt tgtgttttta tctgccacct aagattttta
     6481 ctttattagg tatacaatgc ctatcatttt cttttatttc ctatatcatt gttttaattt
     6541 tcctcagttt catagtatgg atgttttatc ttgtcaaaaa ataaatgaat gtttcatgaa
     6601 cagtacttca tcacagccta atttttttct ttatgctatc gttatattaa agaaatcaat
     6661 cagaagggaa agaaagtgat gaaaaaagtt ttattagtgg taaaccccag ttcaggcggt
     6721 gaacaagcaa aggaatttga gcaattagcg atagcaaaat tggaatcggt gtttgatgaa
     6781 gtggtcgtct tgcatacaaa aaaagcaggg gatgcaaaaa actttactcg cgaagcggct
     6841 acggaagggt atcatagtgt gtttgtcatg ggcggagatg gaacagtcaa cgaaggaatc
     6901 agtggaatcg ctgaacaaga acatcggcca aatttcggat ttttcccatt gggtacggtg
     6961 aatgacttgg ctcgagcatt agggataccg ctagaacctg aggaagcgat caatcatttt
     7021 tcgattgaat cagtcaagcc attggatatt ggaaaaataa atgacgacta ttttatgaat
     7081 gtcgtcgcaa ttggctctat ccctgaatca atcaatgatg tagatgcgga gaagaagacg
     7141 aaatttggaa aactagctta tttcatgtca ggaatcaaac agttagcttc cacacaatcc
     7201 tactcgtttc acgtagaagt tgatggaaag aaggaagaaa ttgagagcag taccttgctg
     7261 ataggactaa ctaactcagt cggcggtttt gaaacattac ttccaaatgc aaaagtggat
     7321 gatggtaagc tccatttggt ttatttaaaa gattcttctt tgatcgatac gcttaaaaca
     7381 ttgccagacc tattaaaagg agtagatgaa tctacgaaaa atctagccta ccttacttgt
     7441 gagcaattag ttgtgtcatt agctgatgag gaagaattga cgacgaatgt tgatggagat
     7501 gaaggggata aattacctgt gacaatcaaa gtattgccct cacatttaca ggtttattgt
     7561 taaaatggag gttgtgaccg aggagttggc gggagaacac cgcttgttcg ggataaggga
     7621 ctgtgacaag acttttgtca cagccttttt tttgtacaaa aaagaaattt acacctaata
     7681 gaaaaatttt tccataaaaa tacatatttt aaatatgaaa gtacaattgg aaatagatta
     7741 aaacgataaa ataaaattgt caaaacgtca gagaggatgg tggagaatga atcaatgtgt
     7801 aaaaaagctg attgcgtcga ttttgtttgt cttccctctc ttgatggtga catccgtttt
     7861 tgccgatgta gagaaaacaa acgaatcaac gcaatcagag aatcaaagtt ttgacaagac
     7921 tactttttca gaagaatcga ttacttctga aactacagaa tctcaaagtg aggcttctac
     7981 taataaaagg caagaagaaa ataagacaag tgagacctca gaagtagctg aacaaacgtc
     8041 tgatagcagt gaagcggcac tggcaagagc tgtttcagcg gatgggtaca gcgaagctgc
     8101 cacaacagaa gaactggcgc agcttctcgc agacgaatca gtaactaaga tccgactgat
     8161 tcagcctttg actttagaca gggaattaga gatcaaacga gatatcgtta ttgattttgg
     8221 tggattcgct cacaatttcg gcacacacca tatttatatc aatgagatac ctcagtcgat
     8281 cgagttccag aattttaaag gaacagctgc acatccaggt ggattgacac caaatatcga
     8341 tggaaatgcc attatcattg cttatatgac cgatttttgg ggaagcagat atcattttac
     8401 gggagaaata aaatttaccg ggacacttga tttgtatgat gggtccaaat taggactgat
     8461 ttacgctcct cgtgcaactg tcacattaga tggtgtttcc ggtgtgttgg atgtccaacc
     8521 agtgaaggaa ggactgaatg ccgctccggg taaagcctat ttttgtagaa gctatcaatt
     8581 gaatgtgatc aatggtagtc aactttatgg accgtatcta gggaaattct acggttttct
     8641 agattcagga gatgagactg gtagtggaaa aacagcgcca ggaatccata tcttaagcgg
     8701 ttcaaaggtc tctcttgatt atgatcgggc agatggcgtt agcgatgaag gagaggcagt
     8761 cgatacgcta cctggtaatg tcatgtttga agtcagtggt tcgggaagcg aattttcggt
     8821 aaatacaaag atcaatatta ccaatgacaa caatcgcgga atcatccaaa tgagaggttc
     8881 aggatcgaaa gtcactgtat cgaatggtgg gaaaatcacc atcaatacag caactacggg
     8941 cggatttcgc ttgcaaggag attctagtca aatccatgta tcaagtggtg gacaaatctc
     9001 ggtcaaaatg gctggagatg acgatcaacc cgggaacaat gggatgcgtt ttgtaggaaa
     9061 aggcctttct ttgatcgtag aaggagccgg atcagttatc gacatcgata agcagagtgg
     9121 gcgttccagc gcagttcgtt ttgagaatgg tacgcaaaag ctgacagtaa ccaatggagg
     9181 ctcattgagg gtaagaaata caggtgatgg caaatcttac gtgaacagac tcaatcaagg
     9241 aaatcaagcg atccaatttt atgacgcttc tggttttcta gaaagtccag gttcagctga
     9301 ttttacagtc actggagaaa aatcaaatat cgatatacgc gcagattccg gagcaactgt
     9361 agatacaact ggagatattg atttaaattt tacggcaggt gaaaaaactt atttagtcat
     9421 ggtcgggaaa acaggggaag ataagaccgg catcatcagt ggggatgtgt tgaatgtgga
     9481 cttagaatct cctacctatt ttgatttcca aaacaaacgg attggaagcg agaccaatgg
     9541 cataggcggc tgggtattcc aaggaaatgg agattcttct ttaaagatcg cccattcaga
     9601 aatcgcttta tggcgaaaaa caggcgcaac attcgataac tttgaaaaag acccggatta
     9661 tttttctgat atgacagact tatcgatcaa tgggaccgac ttaggcaatt tcgtatcaag
     9721 ccctagtgca ggtttaacaa atgaatttgc tcagacaggt gccggaatga aaaattataa
     9781 tcgaatcagt gccaataatc agctcccgat gattgaatcc ttaagagtgc caacgaatgc
     9841 agataaaaaa atctatgggc atgtcgttgt tcccgaaggt gtcgaggcaa agccaagaga
     9901 tgcttggaca ggcgaagtag aagtgacttt acgtattact tacgcagata cgacgaaatc
     9961 atctgtagaa attactgctc tcacgcaagg acaaacagat gaaaatgaag gggcaggata
    10021 taacccatgg ggagaaggcg aacaaggcgg cttgttcgaa gttcaagtac ccaatgatga
    10081 gagattaaaa acggaggatc aagtgcaagt tgttgctgct aaaaaagtaa gaggtggcca
    10141 agaagtgaca gaactggact ctgccaaaac gacagtcgat gtgattcctc ctgaacctgc
    10201 taatttaagt accacattga tcaatgcagc ttctcgcacg atcacaggta caggtgaagc
    10261 aggagccttg gtttcattga aaaaaaatca agctgttttg tcagaaacaa ctattgatga
    10321 tacaggggaa tttgttcttt ctatcccaga aaatcaactt tttgcaggag atgtattaga
    10381 aattgtatta aacgaccaag caggagaagc tactgctaag ggtgtgcaga acaagccaag
    10441 taccaacgat gaaattggga atcacaatcc aagtgatacg ccagttgttt atcatgatgc
    10501 cccgcctttt gcacctgcta cgaagatcgt agtagaaggt ggtctctcat ttcttgcccc
    10561 ttccaaactc gattttggtc agatcaaagc aactggtttg acacaagaag catttggcca
    10621 aatggatata caggaaaaat tatggattta tgatcagcga gaaacgaaaa atccgtggat
    10681 cttaacgata aaattatctc agcctttcac aaatgatgca ggattttcta tgcaggatgt
    10741 cctatatttc aagaaaggag aggagtatca attagtgaat gaagaagaac tggaaattgt
    10801 cagaggacaa aattcaacga atgtgcaagc agattattca gaatatttaa ataacggtag
    10861 ggcgtttaaa cttgtcttaa ccaaagaaaa tcagatagct ggtaattact cagcagagat
    10921 aacatagaca ttagcagatg cgccgacaga ataaagatca aggaggatga ttgatgaatc
    10981 gaatatggaa taaaagtcat cgaaacaact gctctttact tatgatcctt ctccttggat
    11041 tcatcctttg gcagacacct gtccatgcct ccgaattcaa ttttgcggta gattttgaat
    11101 taccggctaa tcagattgat aagaacaaaa catatataaa tgtaaggatg gagcctggga
    11161 aaacacagga aatcccctac acggtcttta accgaacaga taaatcagta acagttgaga
    11221 cattgatcca ctccgctacg acgaattcaa acggtgtgat cgaatatgga gaatcaaaaa
    11281 gtaaatttga tgaaacatta agttatgata tgaaaaactt agcaaaaaca gtgggagaaa
    11341 taactgttcc tcctcaaagt gcggtcactg agacactttc actgaaagcg ccagaaaaag
    11401 agctggctgg tgtggtcgca ggagggatca cgtttcaaga aaaaaaagag aacgtttcta
    11461 acaaaagtac agttggtgtc gtcaatcagt tttctcaagt gaaagcaatc ttgatttgaa
    11521 atagcgaaaa agagtcttcc ccagatttga aattaaaaaa agcttatgcg gatcaaagcg
    11581 atggaagaaa tgtgattaag gcgaatcttc aaaatccaaa tgcttcttat atgttcggct
    11641 tgactacatc ggttgaaatc tacaaaaaag aaacaaaaga gccttacctt tcaataaaaa
    11701 aagaaaaaat agatgtagca cccaatacaa attttgattt acttgtccca ttagaaggaa
    11761 aaaaattagc tccgggacat tatcaagcgg ttgtgaaggg tgcttggaaa gaaaaacgct
    11821 ggaatctcca taccgatttt gaaataactg atgatgcagc ggaccaattg aatgaaaagg
    11881 atattgattt aaaaaatcag ccagaaacat ttgactggcg atggctgata cttgttggag
    11941 tcattcttat tggcttattt gctggattat ttattttaaa agaaagaagg aaaaagaaat
    12001 gaaaaaaaca attgcattaa gtaccgttct agctggaagt ctattattat gtacacaaag
    12061 tgtttttgct gagaataaga gctcagccac tacagaagga acgatttctg tgaccaaacc
    12121 agatgctgca aatcctgaag acgttgttcc agttcctcct gttgatccaa atgatcctga
    12181 tactcctttt acacctgacg aaccaaatca gggaacatcc ggattactga cgattgacca
    12241 agctccagat cttgattttg gaacaatcaa gttaggtaca gcaaaaacag tatatgctgc
    12301 attacaaagc ggaacggaca gtactggagc acaaacacaa gtacagaatt tcgtacaagt
    12361 aacggataaa agcggaaatt atgccggttg gaaattatca gtcaaacgaa cagaatttat
    12421 tggtacaaca tctgatacga ataaactaac tggttcaaag gtgaaattca aaaacgccgt
    12481 gattcgaaat agcgcaacta atagcagtgg aatgccatct accgtttctg gaactggcgc
    12541 tgaaggaatc gaaatcccag cagatcaggc agtagatcta gtaactgcag gagcaaatga
    12601 agggattggc acatggattt attcattagg agagaatgtg gaacaaggga gacaatcagt
    12661 ggaattattt gttcctgtat ccaattatgc tgtagacaac tacgtttcaa cattcacatg
    12721 gtcgttaaca gatgctccta cagaagagga agcgtaaaat catatcaacg acttcaggga
    12781 gtggagccga ttcaaggccc actccctgaa atctgctatg cttacgtctc agctcttatt
    12841 catcaactcg aagcagtttt ttggaagtca tggaaacgtt cctttgtctt ttcaaagatc
    12901 tttcgaccaa cgagtaagga gtcaccgttg aaaaattcta acacataatg agcagaatca
    12961 attttctgca attgcgataa gttgatacag taagatttta aaagtaaaaa gtaagcatac
    13021 ttttctaact ctgtcttcaa ttgttttaaa gaagtattca ctaatttatc ttctgtagtt
    13081 gttttgacca taacttggtt tttcaaacct ttgatacttt caatatataa aatttcccga
    13141 ggatacagga cgagacttcc acctttttga tcaataaaga ttttatcgac atttacgatc
    13201 tgctgttctt tccaattgat ctgttctagc acttgacgaa tttgttcaga taaattcctg
    13261 tcttttctga tatatccaat cggaaggatt ttccgattga tgatctctac tgcaaaattc
    13321 gtataagcgg tgaaaaaaac aatattacta tgtgcatttt ttttacgtat ttgttcggcg
    13381 acatcaatac ctgtgagtga ttgcttcaag tcgatatcga taaagaaaac atcgtgatta
    13441 tcaatagtgc ccaactggat caactgattg atttgagaga tagaagtgag ctgcacgata
    13501 ttctcgatat gatgattgac caattctctg atgatccatt cgctgtgaaa ctggttatct
    13561 tctatgataa aatacttttt catatgcagg ctcctattaa aaaaatatgt gtacattttg
    13621 aaacaaaaca atggaggaat aagatggtcg caacagtgac actttatttg acctttactt
    13681 tgattttaga tttaggtatc tatttattct tttcgaaaca actgctagta aaaaacagct
    13741 ttggttgtat agctttgatg gtggtgacac ttttacttcc tatccctaaa gtgagctttc
    13801 cttacctaat tggcttatta ttgatgtttc ttttttattt tactacgaaa aacctgttta
    13861 tcagctgttt gatcagtaat cttttttttg ctatcactgg actcagcttt tttctggttt
    13921 ttgatctgat ggattattta ggaaagagac atcttggaac agcaagtttt tttgcactac
    13981 ttatcgcatt tattttctat ctattagtaa aaagtataga caaacagacg agatttctca
    14041 acttgctttt tacttatcca actgaacact attttggagg tatttgtatt ttattaagct
    14101 ggggcgtgat ttacttggta ttgggaagtg ttcacttccg ctcatctaat tatctgcttc
    14161 tatctctttt gtcgat
//
LOCUS       DACDIH010000066        13679 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDIH010000066 DACDIH010000000
VERSION     DACDIH010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.066"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..902)
                     /locus_tag="IV281_002122"
     CDS             complement(<1..902)
                     /locus_tag="IV281_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP8781098.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(926..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1185..2153
                     /locus_tag="IV281_002123"
     CDS             1185..2153
                     /locus_tag="IV281_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8781099.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2266..2544
                     /locus_tag="IV281_002124"
     CDS             2266..2544
                     /locus_tag="IV281_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8781100.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2662..2898
                     /gene="secG"
                     /locus_tag="IV281_002125"
     CDS             2662..2898
                     /gene="secG"
                     /locus_tag="IV281_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP8781101.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3010..3756
                     /locus_tag="IV281_002126"
     CDS             3010..3756
                     /locus_tag="IV281_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8781102.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3759..6116
                     /gene="rnr"
                     /locus_tag="IV281_002127"
     CDS             3759..6116
                     /gene="rnr"
                     /locus_tag="IV281_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP8781103.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6138..6602
                     /gene="smpB"
                     /locus_tag="IV281_002128"
     CDS             6138..6602
                     /gene="smpB"
                     /locus_tag="IV281_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP8781104.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6904..7623
                     /gene="atpB"
                     /locus_tag="IV281_002129"
     CDS             6904..7623
                     /gene="atpB"
                     /locus_tag="IV281_002129"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP8781105.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7676..7891
                     /gene="atpE"
                     /locus_tag="IV281_002130"
     CDS             7676..7891
                     /gene="atpE"
                     /locus_tag="IV281_002130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP8781106.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7982..8506
                     /gene="atpF"
                     /locus_tag="IV281_002131"
     CDS             7982..8506
                     /gene="atpF"
                     /locus_tag="IV281_002131"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP8781107.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8493..9035
                     /locus_tag="IV281_002132"
     CDS             8493..9035
                     /locus_tag="IV281_002132"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP8781108.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9058..10614
                     /locus_tag="IV281_002133"
     CDS             9058..10614
                     /locus_tag="IV281_002133"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP8781109.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            10628..11530
                     /locus_tag="IV281_002134"
     CDS             10628..11530
                     /locus_tag="IV281_002134"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP8781110.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRAVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11553..12959
                     /gene="atpD"
                     /locus_tag="IV281_002135"
     CDS             11553..12959
                     /gene="atpD"
                     /locus_tag="IV281_002135"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP8781111.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12973..13395
                     /locus_tag="IV281_002136"
     CDS             12973..13395
                     /locus_tag="IV281_002136"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP8781112.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4521 a         2448 c         2980 g         3730 t
ORIGIN      
        1 ctttcggaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
       61 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccctgagt ttccacagcg
      121 gtgaagcttt tgaaaccttc acagatggcc ccctcgatga aataagcaac agctaacgga
      181 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
      241 gtgatcgctt ttaaatactc tccctcttca ggattcattt gacagcagta ttcgaggata
      301 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
      361 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
      421 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
      481 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
      541 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
      601 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
      661 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
      721 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
      781 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
      841 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
      901 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
      961 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
     1021 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
     1081 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
     1141 gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
     1201 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
     1261 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
     1321 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
     1381 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
     1441 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
     1501 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
     1561 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
     1621 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
     1681 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
     1741 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
     1801 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
     1861 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
     1921 catcagctac gagacagctt ggcagcaacc atctgccttt attagtcatc catggtgaaa
     1981 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
     2041 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
     2101 cctattggac agtgattttt tcatttattg aacaaagaat aacgatggat taattttctc
     2161 ttcttttttg taagtaattc gaggataaat aattgtttat agatctggag actgctaaaa
     2221 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
     2281 gaaaaaaatc gtcttgcttt attcaatgat gagcaacaag agattggaga aatgacatgg
     2341 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
     2401 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
     2461 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
     2521 caagacgtct tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta
     2581 ttccatttat atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc
     2641 ttttgggaag gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg
     2701 attgttatgg tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca
     2761 tttaccggcg gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc
     2821 atgcagcgat caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca
     2881 tatttatctt cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt
     2941 gcaaagagaa cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag
     3001 gtgagacgag tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt
     3061 ttgctgcttc acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg
     3121 gaaaaagcag attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca
     3181 tatgatatct tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat
     3241 tttttacagt ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc
     3301 tttgctgtac gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct
     3361 ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg
     3421 aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta
     3481 gctgaacaac aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca
     3541 gatatccaac agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat
     3601 ggcgtatttg aaactgcacg caagttgagc cgtcagcgag tgacacttca atggtatcct
     3661 gaaagtggtc atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg
     3721 gaatttttag aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag
     3781 agaaaattct ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg
     3841 ctgaaggttt agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc
     3901 aaatggaaag agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa
     3961 aaccaatctt gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga
     4021 ttgatcccga agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg
     4081 gagacaccgt agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag
     4141 aggggaaagt agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg
     4201 cttattcaga agaagaaata caagaaacag atctttacgg cgttgttatc ccgaaagata
     4261 gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa
     4321 gtattgtgct gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag
     4381 ggataatcaa gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg
     4441 tcgtggctaa tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag
     4501 ttccagatca aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca
     4561 ttgttacgat tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat
     4621 tagataacgg taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac
     4681 aaggcagtca attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc
     4741 gagtgattcc gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg
     4801 ttccaaggct gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc
     4861 atgatatttt cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc
     4921 aaatcttaga agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt
     4981 tccaagaaat gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga
     5041 tttcttttga ggatcgcgaa gcgaaaatcc ttgttgatgc tgaaggacac ccaaatgaca
     5101 tccagctaag aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca
     5161 atgaaacggt agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg
     5221 aacagccaaa agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc
     5281 tggtccgagg aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag
     5341 tagaagacaa accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag
     5401 cacgctattc agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact
     5461 ttacatctcc tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca
     5521 gtgaagataa aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag
     5581 atcatagttc taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga
     5641 agaaagcaga attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg
     5701 ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca
     5761 atgaattgaa acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa
     5821 gaactggtct gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc
     5881 cagatgaacg agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac
     5941 gtccaaaagc acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct
     6001 cagaaaaaaa tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga
     6061 aaaaagggaa aaagccattt tacaaagatg tcaaaaagaa gaaaaagaaa aaataaaatg
     6121 agttggagga aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag
     6181 ctcgccatga ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg
     6241 agatcaagtc gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca
     6301 acggggaagc tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta
     6361 atcatgatcc gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg
     6421 aaaacgaatt gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg
     6481 gctatgccaa ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag
     6541 atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat
     6601 aatttcatta tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg
     6661 ttagcattta atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg
     6721 tgcatgggga aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga
     6781 aaatgttttg aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg
     6841 aaaaaaccag atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat
     6901 ggattggatg aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt
     6961 tgtataatgg tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga
     7021 aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt
     7081 cgaggaatcg taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca
     7141 tttacattgt ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc
     7201 ttacctaacg aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact
     7261 ttggcattta taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa
     7321 ggatatttcg taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt
     7381 gaagaattta caaatttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg
     7441 gaagttttgc taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca
     7501 ttagcaattc cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa
     7561 gcatttgtct ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa
     7621 taatactcgg ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa
     7681 ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg
     7741 tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt
     7801 ccgtacaaca atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt
     7861 tatcgccttg attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat
     7921 cgtttgttac tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc
     7981 tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt
     8041 tgtcagcggc tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat
     8101 cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca
     8161 atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg
     8221 ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa
     8281 tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat
     8341 cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct
     8401 tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat
     8461 caatcaatac attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa
     8521 gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct
     8581 atcaagatct cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt
     8641 taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg
     8701 gttatgatgg gatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg
     8761 accttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat
     8821 tgggaagtgt cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga
     8881 atgttgcaaa aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta
     8941 ttattggtgg cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc
     9001 aattagaaaa aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg
     9061 gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac
     9121 gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca
     9181 catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga
     9241 atggcgcaaa acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc
     9301 attcgtgaag gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag
     9361 gccttgatcg gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc
     9421 gttactgaca aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct
     9481 gttaacgaac caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc
     9541 ggacaacgtg aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat
     9601 acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa
     9661 gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca
     9721 atcgttgtga atgccggtgc gtctcaacca gcaccattgc tttatatcgc accatatgct
     9781 ggtactgcaa tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat
     9841 gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca
     9901 ggtcgtgaag cttatcctgg ggatgttttc tacttgcact cacgtttatt agaacgtgca
     9961 gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca
    10021 caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa
    10081 atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta
    10141 tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact
    10201 ttacgcctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac
    10261 ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa
    10321 caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca
    10381 catggattct tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc
    10441 gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt
    10501 ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca
    10561 aaaggaagta attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt
    10621 gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac
    10681 gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc
    10741 ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac
    10801 tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag
    10861 catgctgctc agccgtgctg tgaagaaaac tggttatatc gtcatcactg cagatggcgg
    10921 tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga
    10981 ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt
    11041 taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt
    11101 tgatgaagtc cgcaaaatcg taaatatggc aacaactatg ttccaaaacg aagtattcga
    11161 tgaattatac gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga
    11221 aaaaatgctg ccaatctcag acttagatcc agaagaagca acgactttcg aacaagaata
    11281 tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag
    11341 tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc
    11401 aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa
    11461 ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc
    11521 actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa
    11581 tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg
    11641 cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag
    11701 aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag
    11761 gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag
    11821 gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg
    11881 cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag
    11941 aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga
    12001 aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc
    12061 ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc
    12121 gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca
    12181 tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt
    12241 tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca
    12301 acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt
    12361 ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattacaa gaacgtatca
    12421 catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact
    12481 acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac
    12541 gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg
    12601 ctttagcacc tgaaatcgtg ggggaagaac attataaagt agcaacagaa gtccaacacg
    12661 tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat
    12721 ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa
    12781 actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa
    12841 cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt
    12901 tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga
    12961 aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc
    13021 tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg
    13081 cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca
    13141 gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat
    13201 aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt
    13261 gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat
    13321 acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta
    13381 tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag
    13441 gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca
    13501 aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca
    13561 gaggttgtaa aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct
    13621 catattttgc cgttttttga gctaataccg agggtcagat gattgaacac cgtttattt
//
LOCUS       DACDIH010000067        13599 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDIH010000067 DACDIH010000000
VERSION     DACDIH010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.067"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..380)
                     /locus_tag="IV281_002137"
     CDS             complement(<1..380)
                     /locus_tag="IV281_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8781113.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIG"
     gene            526..1329
                     /locus_tag="IV281_002138"
     CDS             526..1329
                     /locus_tag="IV281_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP8781114.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            1507..2454
                     /locus_tag="IV281_002139"
     CDS             1507..2454
                     /locus_tag="IV281_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8781115.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(2563..4110)
                     /locus_tag="IV281_002140"
     CDS             complement(2563..4110)
                     /locus_tag="IV281_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP8781116.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(4212..4565)
                     /gene="tnpB"
                     /locus_tag="IV281_002141"
     CDS             complement(4212..4565)
                     /gene="tnpB"
                     /locus_tag="IV281_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP8781117.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(4555..4749)
                     /locus_tag="IV281_002142"
     CDS             complement(4555..4749)
                     /locus_tag="IV281_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781118.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            4833..4937
                     /locus_tag="IV281_002143"
                     /pseudo
     CDS             4833..4937
                     /locus_tag="IV281_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(5005..5298)
                     /locus_tag="IV281_002144"
     CDS             complement(5005..5298)
                     /locus_tag="IV281_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP8781119.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(5326..5991)
                     /locus_tag="IV281_002145"
     CDS             complement(5326..5991)
                     /locus_tag="IV281_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP8781120.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(6131..6763)
                     /locus_tag="IV281_002146"
     CDS             complement(6131..6763)
                     /locus_tag="IV281_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8781121.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(6770..7837)
                     /locus_tag="IV281_002147"
     CDS             complement(6770..7837)
                     /locus_tag="IV281_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8781122.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(7834..8565)
                     /locus_tag="IV281_002148"
     CDS             complement(7834..8565)
                     /locus_tag="IV281_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP8781123.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDIEVIRI"
     gene            complement(8733..9212)
                     /gene="greA"
                     /locus_tag="IV281_002149"
     CDS             complement(8733..9212)
                     /gene="greA"
                     /locus_tag="IV281_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP8781124.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(9348..10523)
                     /gene="mltG"
                     /locus_tag="IV281_002150"
     CDS             complement(9348..10523)
                     /gene="mltG"
                     /locus_tag="IV281_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP8781125.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            10688..11848
                     /locus_tag="IV281_002151"
     CDS             10688..11848
                     /locus_tag="IV281_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8781126.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            complement(11888..12835)
                     /locus_tag="IV281_002152"
     CDS             complement(11888..12835)
                     /locus_tag="IV281_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP8781127.1"
                     /translation="MLNYLITGGAGFIGSTLANYLGKENNVVVIDDLSMGKKENLDES
                     KHITFIEGDVADKNLMEKVMKEYHFDYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(12868..>13599)
                     /locus_tag="IV281_002153"
     CDS             complement(12868..>13599)
                     /locus_tag="IV281_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP8781128.1"
                     /translation="VVSIIIVAISSFIVVLKRNGTKKDLKNYILAIVSSIGSVLILFA
                     LFNATKILFQELLPHLKEINFFNFTERHKELMNNSQHINNTQTPDNTLVRKDVAENTD
                     ISNARITIWKSAWEIFKTSWLIGVSPKNVVSYAQSIIPDGYIAKVGIAIHNAYLNVLT
                     STGILGIIPFMAFLIKSFVLNVYRIIYNKCIGKDYHFVYCLVILSYALYAALNNELVY
                     ENTVGTFVFWLFLGRVNSKNNSSYF"
BASE COUNT         4111 a         2775 c         2186 g         4527 t
ORIGIN      
        1 ccaataactg ctactgttag catcaaatga ctatttatct tttctggtga agtgaaccgc
       61 ttgaatgctt caacggttaa aaacaaggat aatatgatca atatgaatga gttgatgaaa
      121 gcactcaata tttctaaacg ctggtagcca tacgtacgtt ttttatttgc ttcttttcgg
      181 gccatttttt gtgccccgta agaaaaaata attgccaatg tatcactaag gttatggaaa
      241 gcatctgaaa caagagacaa actccctgac ataatccctc caataaattc tgataaagtg
      301 atcaccgcat tcaaaaccac tactccaaat agtttttttg atgatctctg tggctgtgta
      361 ggcactattc cttcctccat tttatttata ttggatagtc tttagacaag aataacattt
      421 ttttgatatt tcaccaatca ttcagctgtt tccttaaagc acttgtattt cacttgatac
      481 ttcgctagaa ttgcaagtga tgacactact agatgaggag tacttatgac aacaaaacaa
      541 ttaattatca gttcctttat acgattcaaa gacctaaagg aggcgagaaa tgtcccattt
      601 tggaaagcca ttgtctatct tttagttttg agtataatta tggctttgcc aatctctttt
      661 caagtttttc aagtcttaaa caacattcaa ttggatgggc aaaagattgc tactcgcatt
      721 cctgatttta caatccaaaa cgggcagctt caaaccgaag aaaaagaagg attcatttac
      781 caaacaaact ccattatctt cacatttgat ccagagggaa aacgaacaga acaggacatc
      841 tctactgatt tgatggggaa ttttgttagt gtcgggatgt taaaaaacaa acttatcata
      901 gctcttccta atacaggcac gacttctgct ttgttgaaca ataaccaatt agaacttccc
      961 tacaccaatg aatctttaaa aaacttaaca ggtaagcagc tccgttcttt tttaagtgaa
     1021 gcatctatac cactttggat aaaagtcctg acatttatat tttctattta tcctagtttt
     1081 ctgaatctgc tgatcacttt gctatttacc aacattgctg cttttctcta tgcacgcttt
     1141 cgactaacaa aagctacttt tcttgattgt ttaaaaacat tgatctattc aatttcattg
     1201 cctactataa ttgcaacaat cttgatggtc tttcttcctt cttttgacac tagcgccttc
     1261 attgcttttg caggaatctt tatttttgct caagctgtca aaggatgggc aaaaatatcg
     1321 atcaattaag aaataaagac aactcgttat ttctacttca atagaccctt ttagaggttg
     1381 tgacattttt ttgtcacagc ccctttttta tttgaaaaat atttctttct caaaaaacaa
     1441 gcaaggcgct tcaaagttct tcttgtatgc gctacactta gagtaaagaa aggcgggaaa
     1501 ttaactatgt cagaaagaat catttatgca gatcgtccat ttcgtgatga atttatcgaa
     1561 gaaatccata ctatagcgcc tggttatatc tttaaaacag aattacaacc agaggatcta
     1621 caaaatgtag agatcagttt gggctggaac tcagactacc aagaaaaact attagcctct
     1681 tctaccttga aatgggtcca atctatctca gctggtgttg atcagctacc ccttgagact
     1741 ttttcaaaga aacatatttt actttcaaat ggtagtggca tccacagtga atcgattacg
     1801 gagcatgtct taggaatcat ccttgggtat tctcgtggtc tttttcaagc acaaaaggca
     1861 cagagtgaca tgaaatggtt aggttcatcg gtccattacc aatctatcca agggaaaaaa
     1921 atactaatca ttggaactgg ccatattgga aaaatgattg caaaaaaatg caaagcatta
     1981 gacatgaact gctatggaat caatacatcc ggtcatccag cagaaggaat ggaaaagact
     2041 ttttctttaa atcaactaaa ggaagttctt cctgaagtag atttcgtagt caatatctta
     2101 cctctaacgg aggaaacaac tgggctcttt gatcagcata tgtttgaaca ctttgatcca
     2161 aaatctgtat tcatcaatgt tggccgtggt gcttctgtta agacacaaga tctggtgcag
     2221 gctttaaata accagcaact ttcctttgca gcattagacg tttttgagga agagcctctt
     2281 ccacaagatc atcctctttg gaaaatggac aatgtactga ttacttccca cattgcaggt
     2341 ttgacacctg attttcaaaa gaaattaatg gctatttttt taaccaatct gaaaagctac
     2401 tttgccaccc atgagcttca aaccaatcaa gtaaaattgt ctgcaggata ttaatagctg
     2461 aaaacaatat tttcaaaaga aaaaaggtaa gcgccccata gaacagtacc tgtacgtcaa
     2521 ataacccgac ctatagaata gaatcgggaa tgtttggcgt ttttagttac gagaaaaatg
     2581 ttgttgtaca tccttgttcc aaggcaacag ttgatctaaa gtttctgctg aaagtacggg
     2641 aagatttggt atcttatcaa atagaaactc tagatacttt acaggattca atccgttaag
     2701 ttctgctgtt ttatatacac tcagaatgat tcctgaagtt cgtgctcctt caagacttgt
     2761 ggaatgcaac caatttttcc gtcctatcac cagctctttg atatttcgct ccgcacgatt
     2821 atttgtgagt tctaaacgac catcttcaaa aataggttca aaaccacttc gtagtttaca
     2881 cgcatattga acggctgcat ccaacttgga ttttgcaaca ggatctatcg ttgccatcca
     2941 ctcataaaaa gcaataaata atggacgcag ttcttgttgc cgacagctta atcgcttctc
     3001 aggagaaaag tctttccatt tcttttctag gacaaaccat ttatccaatt gtttgactgc
     3061 ttgagctgcg ggaatatccg tgtttttctt tccttttgga attgcttcga aaaatttccg
     3121 ccgaacatgc gcgaaacaat agacaagtcg tacactttcc acggcattat acccgctata
     3181 gccgtcacag tgtaaatacc ctgtaaactc cttgagaaaa gttttcggaa catctccagc
     3241 acgactttcg tcgtggtgat aataaacgat gggatgctga ctgtcttttc ctgtacaaaa
     3301 ctgccagtaa taggtatctg ttttggcact ttcaatcaca cgataacttg tttcatcccc
     3361 gtgtagaatc tcttcttgat taagatagac tcttaatcgt tctcctaaag ggtctaacgc
     3421 gttttgcact gctttaatat gccaattggt gatattatct ctcgaaatat ccaacccatg
     3481 tgtttgtttc caatagttct cttgccgata ggcaggaact ttctgaccaa atttcatccg
     3541 gatcgtttcc gccactaaac tattggaacc taagctatta gaaatcaaag gtttaggaac
     3601 gatcgctttt ttaaaagaag aaagtccttc tttttcgcag cttttacaac aataggtatg
     3661 ttgataatgg gtgagacaat ccaacatagc ctgatggaaa cgaatttctt ctcgtacttt
     3721 tgtccgtccg atatctttca tctgtgaacc gcaatgttca caaaattgtt cttccaaagg
     3781 aaggagatgt tctttctctg ttataggtaa gtgtttgatt ttgtctgctt tcgttcctac
     3841 aactcgttga tgtcctttta ctggaataat ggtggcagct gcttctggaa cacttacaga
     3901 aatttcttca tcactaaaaa gattcaattg cccattcgtt tcctcaaaga gagtctcttt
     3961 agagcgtcca aataatttgt tggttaaata attgaccttt tcattcaata gttggatttc
     4021 agttgtaaga ttctgaatgg tttgttgttg tgcttgattg gttttatggg cttcttgaag
     4081 caactgaagc aacgtatctg tagtttccaa aatttttcac caccttgaat cgaactgatt
     4141 ttgttacaga ataccacaag aaacgcacaa gaaatagggg ttcaacacat tttttgtgtt
     4201 aaccccccag cttaaaaaac tccttttttt cctggcaaaa tagctcgagg ttgatcgata
     4261 cttaatcctt ctaacaacca tcgaacctgt tggttcgtta aggttcgaac ttcgtctttc
     4321 gtccgaggcc attttaactt gccgttttct atccgcttat aacagagaag aaaaccatca
     4381 ccgtcccaat agagaagttt gaaacggtct tgacggttcc cgcaaaataa aaaaacggca
     4441 tcttcgtaaa gctcgagttg atactcttgt tgaataagac tggcaagacc atcaattcct
     4501 tttcgcaaat ctgtttttcc gcacacaata taaatatgtt tggtttgaag taaatcataa
     4561 agcattggac aacagttctt tcaaaatagc ttgaagaata taattgttaa taccgttata
     4621 gatcaaaact tcagaaccat tctctctttt gatgcgcatc gctaacttgg cagccgcggg
     4681 tttacctttt tctggtgcac tcttttcgtt tcctaattgg acaggaacaa tctcatttaa
     4741 tttatacata gaaaacctcc agacttcttg atagctcaag tatatcttga ggtgcatttg
     4801 ttagtaaggt actgttctat ggggcgctta ctgattttca aaagaaatta atggctattt
     4861 ttttaaccaa tctgaaaagc tactttgcca cccatgagct tcaaaccaat caagtaaaat
     4921 tgtctgcagg atattaatag ctgaaaacaa tattttcaaa agaaaaaaga gaaggtgaca
     4981 aaaattgtgt caccctctct ttttttattg ttttttaaag tgataggttg gctcctccag
     5041 gttgggatca taatcaacaa gtaaatcata tcctttaaag aaccattcat ctgcttcctc
     5101 gataaaaaaa agtgtcccat ccatttccac tttctttaca ggatgttctg gctgttcaac
     5161 tgacattcca atcgaaaaac catcatgaac ctctgttttg ccgtagactt ttccaaaaaa
     5221 acgtattccg taagtttctg gcagatcaat ttctgagtca aaccactttt gtgcttttgg
     5281 cgttaccgtt aatttcatcc caacaaccta ctttctcttt tctttctatt gattcatctt
     5341 atcgtctaga atatccacat cgcctgtctc tccaataacc agtagattat cattttcacg
     5401 aacatattca tctgctgcag gagaagcgat aaccgtttga tctactcttc gtatggcgac
     5461 tactgttaag tcaaaacgtt gcctgaaatt taattcagct aatgttttgt tgaaaaattt
     5521 aggattggag actcggattt ctgctaaaga atatttatct gacagctcta agtagtctag
     5581 aatattcttt gatactaagt tatgtgcaat ccgtatcccc atgtctcgct cagggtgtac
     5641 gacacgatct gctccgatct tctctagtac acgggcatgg tactcatttt gggcttttgc
     5701 taaaatattc ggtacgccca tttcctttac catcagagta actaagatgc tggcttggat
     5761 atcttctcca atagcgacaa ttacatgatc gaaatttctt atacctaatg aacgaagtgt
     5821 catctcatcc tgtgcatttg ccacaacagc atgggtggct atattcatat attcattgac
     5881 tcgatcttca ttactgtcaa tagccagtac ttcttgtcca gcttcgatca gtgtacggca
     5941 gatgcttccg ccaaatcgtc caaggcctac aattgcaaaa ttttgtttca ccattttcct
     6001 cctttagtat cttcttttct cagtataaca aagcctttcc aaaaacaaaa gcaagcatca
     6061 ttattgtaaa aaagccaaga aaaataatag ttattttctc agcttttgat tatcctcttt
     6121 atcatcgttt ttatttcacc aaaccatgtt taaacgcata gatagctgct tgagttcggt
     6181 cttctacctc taacttagct aatatattcg acacatgcgt tttgactgtt ttcagtgtga
     6241 taaacaattc atccgcgatt tcctgattac ttttcccttc agaaatcagc atcaaaattt
     6301 cattttctcg attcgttaat tcttcatgca atactggatc atttcgccga ctcatttttt
     6361 ccatcatttt tgttgtcact tcaggttcta gtactcgctc gccgcgttgc gtagccctta
     6421 tagcatcagc tatttcatgt gctgtagacg tcttcaacaa atatcctgct gctcctgctt
     6481 caattgcagg ataaactttt tcatcatcaa taaaacttgt gacgataatg attttggctt
     6541 ttggccaatc ttttaagatt gcttttgttg attcgatgcc atccatttct tccatgacta
     6601 gatccattaa aatgacatcc ggtcgaagtg ccatcgcctt ttcatagcct tgacgtccat
     6661 tttcagcttc tccaatcact tcaatgtctt cttggataga taaataagaa gagacgccta
     6721 agcgtaccat ttcatggtca tctactaata aaacttttat catctttatc tatgcctcct
     6781 tcatcaaagg gaccttggtc tccacgctcg taccttgccc tttaaaacta atgattttta
     6841 ctgttccacc aataccagct actcgttctt tgatattgtt caatccataa cttcccgttt
     6901 tggtttcatt catattaaat cctgtcccgt cgtcaatgat acgtaataaa agattgttat
     6961 ctattttatg caaatagact tctaattcat tagccttagc atgtcttaat gtgtttgata
     7021 acaattcttg aacgattcgg aacaagtgat cctcaatgga actagttaat tttacatctt
     7081 ctacctccca ttttagcgaa atctggattt tattctgcaa ttccttcaat aactgctcaa
     7141 tgccttgttt taaacttttt tcttctaaat tgactggacg cagatgaaga agcagcgcac
     7201 gcatctcaga ctgggaagcg ttgatgatct ctgccaccat tttcaactgc ttacggaaca
     7261 tttctggtgt ctcacttttt tctgcttgct ccgtcaatgc cgacatcatc atcatcgctg
     7321 caaataattg ctgtgaaact gaatcatgaa gctcacgagc taatcgatgc ctttctaatt
     7381 ttaaaatctc ttctttggtt tgtccatcta catattgagg acggctagtc ataatctgta
     7441 attcactgga tacttcgatc attttttctt ttattttagt aatttccata tcaatatctt
     7501 tgatgtacag atcatccgac gcgcttggga tcggctgatg caaaagtttg gattcataat
     7561 tgccagaaga aagtgcacga agcttttctt cgattttccc gtattttgcc ttttgaataa
     7621 gtgataacaa tagaaatacg atcaatccta cacctaatga aattgccatc aaatggaaga
     7681 taagcggaac gtataataaa cgcgcacgca gcaattctcc ccaccaatga ctttggttac
     7741 tggcataaaa ataagtgaaa agtgaaaata gaataataag gaaagccgca atccccgaat
     7801 aaacagctag cattgctttg gatattcttc ccatcatatt cgtatcacct cgatatctcc
     7861 aaccaaagta ttggtaatga tcttcaatct gcgaggattt tcatcataat cactgctgca
     7921 gattttgatc tgttcgtttt ttaaggaaaa ctgctcttct tcaaacaaga catttcctac
     7981 taatgtcgca tgctctaaac gaatagccac tcccaggggt actaagatcc gtgttctgcc
     8041 tatccctttt cttacaatca caatattatc atcttttggc aaaagtgtat tgcccaaatc
     8101 gatgatagta tcaccagaaa tgatggcaat gttgatatca tcccattcat agacgtcgtt
     8161 tccaatacgt tggttaccaa ataattgctg tcttttacgc tcattattat gtgtttttaa
     8221 ttgctctgtt tggaccatca tgatctgttt ttttcgccaa aaagcatttt ttgtcaaatc
     8281 tattcctgaa atctctacac ctttcagccc gataaataaa acggccaata caaccatcat
     8341 ccataaagca ggactgttga ctaagctaaa aaaaatgacc aagctcccta agatcaattg
     8401 aaaattttga aatttagtac gaggatattt gcgcatagct agatatatat tgaaaatacc
     8461 gaatattact aaaagcaaaa gtccagtatt gtttacgatt tgccaaacag caaataacag
     8521 cagcagtgct tcgactacca caaaaaaacg ccaagaacta ttcatcgctt tttctccctc
     8581 ctgacacatt ctcatttcat catacctatt tttttgactg ttggctatag gactttagat
     8641 gtaagatcgt tcagtgaaac ctgcaatact caaagagaaa agatctagag tgcgtcatca
     8701 gtaagatgaa ctctctagat ctttcagtca gactacgctt gttctacttt gacgatttta
     8761 actaacatat cgccacctgg tgtagcaatg gttacttcat catttttacg tttgccaatc
     8821 aatgcttgcg cgataggaga atcattggaa atctttccag aaaacgggtc tgcttcagca
     8881 cttccgacaa tcgtgtactc ttcttcatct ccatcaggca actcgatgaa cgtaactgtt
     8941 ttaccgatcg atacctcatc tgcatctacg ccgtcattat cgatgatttg tgcaaaacgg
     9001 atcatatttt caattgttgt gattcttcct tcaacaaatg cttgttcatc tttagctgat
     9061 tcgtattcgg agttttcgga aagatctccg aaaccgcgag cgattttgat tcgttcaatg
     9121 atttctccgc gtttaactgt ttttagttct tctaattctt tttctaattt ttctttacct
     9181 tctagagtca ttggataaac tttctctacc atgtgtaacg tctccttttt tcgataaaat
     9241 accgcaaaag gtaatctttt gaggaatata gtccaaaaaa gattaccacg cttttcttaa
     9301 aatgtaaatg tttttctaaa aatgatcaat gactagcccc tgtttttcta agaattattt
     9361 acatattttt gcactagttc catatgttct tcatacgttt tagcgaaata aacatttcca
     9421 gtgctcgtat ctgctacgaa ataataatag tcattttctg ctggttctaa tactgccttg
     9481 attgcctctt cgcttggatt gtcaaaagga ccaggcccat agccggtatt ggtatacaaa
     9541 ttgtatggtg aatctacagc tgtatcttca tacgtcacca attctttatg ttctcctaat
     9601 gcatataaaa cagagatatc tgactgaagt ggcatatttg ccttgattcg gttaaagaag
     9661 acttgtgcaa tatttttacg atcattttct tttacgcctt ctttttcaac tagagaagcc
     9721 aaagttaaaa cttcttgtac agtcaaattt ttctgtttga tagctgaata atagttttgc
     9781 atgaccgtat ttgttttgtt caccatctca atcacaagat ccttaagcga agtactcgtg
     9841 tagtaatcat aagtagctgg gaataagtag ccttccaaac ggtattttgt gttctcagct
     9901 ttagaggcac tttccaacaa ttctgggaat ttttgataca actcgttaaa gaaagtctca
     9961 tcatttacta aatctaaaaa ttctttcttg tctttaccag ttaccttcgc tacccgctca
    10021 gcaatctgat cgatgtcgta gccttctgga atggcaattt tcccatctgc aacttttgtt
    10081 ggctcagatg tgccgccttc ttgcaactgc ttgccaatct catcaagagt catattaggc
    10141 gctaactgat aataaccagc ttgaaagcca gttaagtttt taaattttgt ataataattg
    10201 aaaacaatcc cacttttaat aatattgtct ttctccaaaa tttcgccaat ctgcttatta
    10261 gaagagccgc ttgggatttc cacttggact aattggtcgt ccgatttatc taaaggcttc
    10321 aacccgctat ctacataacg atatacggta aacccgagaa atccgccaat aatcaataat
    10381 gtaagagcga tcactaaaat gatcttcctc acgatcttat cttctttttt acgcagattt
    10441 ctccgcttgt cgattatatc tgctgtagct gccttactgg cttctttttt gtctttgctt
    10501 gggtgttgtt tgtttcctga caaaacaact cctccttact atcatcttcg taactctgtt
    10561 tattatacat gaaatttcta ctgccgtgaa ttattttacc aaatattatg tcaaaattgt
    10621 aagtggcaaa tgctcttttt gaggattaca atgaaaaata gttaaaaatg gataggaagg
    10681 atttaatatg gcaaaaggaa aacatcaaaa aaaacagcag atctctcttt cttttctcgc
    10741 agttttgttg ctggctgtca gcttcttttc aacaggcatc attacaggat ggcaattaac
    10801 aaaacaacct caaccacaaa tttataaaac ggaacccgca caacttgctt ccaatttaac
    10861 atctaatgag cagatgcttg ccagcaaaat tgatcaatta ctgcaaaaat cagattatat
    10921 cggttcgatt tatgtacgca aaaataatcg agtgatatta gaaaaaggat atgggtacgc
    10981 aaatctacgc ataaaagaat cgaattctcc ttctctttac tatcaaatcg gttcgatcca
    11041 aaaagcgatg actgctttac ttatcctaaa acaaattcaa tctggcaaga taacttttga
    11101 cacaaagcta tctgaatttt atcctcagat tcctggaagt cagcaaatct caatcaaaaa
    11161 tatgctttac atgcgctctg gtcttcatcg aaccgcttct ccaaaaaaac ctatgtcaga
    11221 tagtgagatc attcagtttg cccttcatca tttgaaattt actggttatg atacgtatca
    11281 ttatgaacca ttaaattata cattgcttac aggggtattg atcaaactaa tgaatcagcc
    11341 ttatgaaact cttttaaaaa aagaaatcat tcggccactc catctagaac atacagattt
    11401 ttatgaaaat gtaaaaaatt cccctcagca tgctgtatct taccaaatgt ctgcaactga
    11461 cgattattca aaagcattgg tcgagcctga aacagacatc cgaaatgaat taggcacagg
    11521 aaatataagt atgtctgttt atgacttgaa caaattcttt accagtgttc tgtctggcga
    11581 tattatacca aaagaactac ttttctcatt gtggcaaaat aatggtgata aacatcctta
    11641 cagcggcggt gtttacagtg gaaatgatta tatccttgct cagggaaata tcaatcgatt
    11701 ccatgcagta gcggcgatga aaaaagacac aaaagatgca atcgtcatgg aaagtaatat
    11761 ccaatctgat aaaaacatca aattgccggc cactgattta agaaatcaga tttacgaact
    11821 gatggaaggt gttaatttaa aaagctaagt cgagacaaaa aaaccagcag gatgctggtt
    11881 ttttgtttca atcgatttct gtctttaaat aacgttccat tccgttcaat atatcaaatt
    11941 tgggttgata acctaaagaa cggatctttg aaatatccgc taaagaatca cgaatatccc
    12001 ctgaacgctc cgcttcatat ttaagcgcta gctctgttcc taagatttga tcaattgaat
    12061 gaatcaattc aagtagcgta gtgctttttc ctgtaccgac attgaattgc tgtcctaaag
    12121 ctttttcttc cttagcaaca agtaaaagtg cttgtataac atcttctata taaacgaagt
    12181 ctctagattg gctaccatca ccaaataaag taaattctgt tttttctcct gccaattgtt
    12241 ttttgtatcg gtccactaga atcgaaataa ctccgctata tggagagtta ggattttgat
    12301 ttggaccata aacgttaaaa aatcggacag cactagtcgg aacatcatat aaatgacagt
    12361 aatccaaaac atattgttct gctgcaaact tatcgatggc atatggtgtt aaaggacgga
    12421 tgaccgattc ttcttttttt ggcaacgttg gttcatcgcc ataaactgca gctgatgaac
    12481 taaataccaa tcgttttaaa ccagattgat attttcgtac caattcaagc aataaaagcg
    12541 cactgtcaaa atttacacga tgagtttcta gaggacgttc tacagaatct gccacactag
    12601 ctacagcagc tagatggaaa atataatcga aatggtattc tttcatgacc ttttccatca
    12661 aatttttgtc agcgacatcc ccttcaataa acgtgatgtg cttcgattca tccagattct
    12721 cttttttccc catcgataaa tcatcgatca caactacatt gttttctttt cctagataat
    12781 tcgcgagcgt cgagccaatg aagccggcgc caccggtaat caagtaattc aacatttttt
    12841 ctcctctttt caagataaaa tttattacta aaaatagcta gaattatttt tgctattaac
    12901 tcttcctaaa aatagccaaa aaacaaacgt tcctacagtg ttttcataga ctaattcatt
    12961 gttaagagct gcgtatagtg cataagataa aataactaaa cagtaaacaa aatgataatc
    13021 tttaccaatg catttattat agatgattcg ataaacattc agcacaaaac tctttattaa
    13081 aaaagccatg aaaggaatta ttcctagaat acctgtcgat gttaatacat tcaagtatgc
    13141 attatgaatg gcaattccta ctttcgcaat atatccatct ggaataattg actgtgcata
    13201 agatactaca tttttcggag agactccgat aagccaactt gttttaaata tttcccatgc
    13261 acttttccat atagtaattc tagcattaga aatatctgta ttttcagcta cgtctttcct
    13321 aacaagagta ttatcaggtg tttgagtatt attaatatgt tgactattat tcattaattc
    13381 tttatgacgc tctgtaaaat taaaaaaatt tatttctttt aaatgaggta ataattcttg
    13441 aaaaagtatc ttagttgcat taaacaaagc gaataaaatc agaacagaac caatagaaga
    13501 tactatagca aggatatagt tttttaaatc tttttttgtt ccatttcgtt ttaacacaac
    13561 aataaaagat gatatggcaa ctattattat acttaccac
//
LOCUS       DACDIH010000068        13229 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDIH010000068 DACDIH010000000
VERSION     DACDIH010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.068"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(233..2353)
                     /locus_tag="IV281_002154"
     CDS             complement(233..2353)
                     /locus_tag="IV281_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8781129.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2583..3761
                     /locus_tag="IV281_002155"
     CDS             2583..3761
                     /locus_tag="IV281_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP8781130.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3777..4535
                     /gene="aroD"
                     /locus_tag="IV281_002156"
     CDS             3777..4535
                     /gene="aroD"
                     /locus_tag="IV281_002156"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP8781131.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4558..5043
                     /gene="ybaK"
                     /locus_tag="IV281_002157"
     CDS             4558..5043
                     /gene="ybaK"
                     /locus_tag="IV281_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP8781132.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(5114..6367)
                     /locus_tag="IV281_002158"
     CDS             complement(5114..6367)
                     /locus_tag="IV281_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8781133.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6483..7832
                     /gene="gdhA"
                     /locus_tag="IV281_002159"
     CDS             6483..7832
                     /gene="gdhA"
                     /locus_tag="IV281_002159"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP8781134.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7944..9290
                     /locus_tag="IV281_002160"
     CDS             7944..9290
                     /locus_tag="IV281_002160"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP8781135.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9598..9984)
                     /locus_tag="IV281_002161"
     CDS             complement(9598..9984)
                     /locus_tag="IV281_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8781136.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            10863..11075
                     /locus_tag="IV281_002162"
     CDS             10863..11075
                     /locus_tag="IV281_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781137.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11353..13152)
                     /locus_tag="IV281_002163"
     CDS             complement(11353..13152)
                     /locus_tag="IV281_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8781138.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4346 a         2377 c         2600 g         3906 t
ORIGIN      
        1 cggatttaaa tcatttgaga acatgagaat tagaatcttt ttgatcaatc aattaatcaa
       61 agtaagataa caaaaaatct gagccagaat gaggttcatt ctgacccaga tttgattttg
      121 tacaacccat cagtccttat tgacaaagag ccaattattc taagatttct ctttaccaac
      181 atcatttgac aaagagccta attttgtcgc tgctcctttt aaatttattt tatcattcgg
      241 aagaacttgt ggtcgttccg tgaagttctt ggtacgtttg cgttttataa agatcaacag
      301 ttgattgtcc cttgtgttca ctaaagacac tagttgactt gtcaccaaga gatttttcag
      361 cttcttttaa tttctctaag ccattcttgt agtcataatc actggtatca attgggtcta
      421 atccactatt cgtatagaac cgaagaagat caccattatt gatttggtca gaaacagcta
      481 actgtttatc aactttttct ttcagtgaat caatttcatt ctgtacttcc tcagtaggtt
      541 cagtaatagc ttcaccagtt tcgttactat agatcgtacc accgtagtaa gtatattttg
      601 gtgtaacaaa atcaccatca cggaaagaaa caagctgttc atgatctttc gagaatagat
      661 cttgtcctaa ctgaatataa ttttttgtat ctacacctag taagtgaagt aatgtaggca
      721 aggcatcaac ttgtccgccg taagtgtgat tgattccgcc ctgtgtttgt ccagggatat
      781 ggatcatata agggacacgc tgcatttgtg cattgtcgta gtttgtccaa gtagaacttg
      841 tttttccaac taattcagct agatttttat ttcttgaatt cgagacaccg taatggtcac
      901 cgtaaaggac gatgactgaa ttatcataca agccgctttc tttcaaataa ttgaagaatt
      961 cttgtacggc ggtatctaaa taattggctg tcgcaaagta accgttgatc gtttcatcat
     1021 ttgtattggc taacgggaat cctgcttcgt cattggtgaa ttttgaataa ggataatggt
     1081 tagatacagc aatgaatttt gaataaaatg gttgttgcag acgttccaag tattgtacgg
     1141 attgttggaa aaatggttta tcatgcaacc cgtattggaa cgaattgtca tcattaacat
     1201 cataataaga agaatcaaag aaataatcat atcctaaatt cttgtatgtc tcgtttcgat
     1261 tccagaatgt accagcgttc ccatggaata ccgcactagt gtaaccttgt gtttgactta
     1321 aaatatcagg tgcagcttgg aaagtatttt ttccgcctaa ttgtgtgaac aaggagcctt
     1381 gattcaagcc aaacaatgaa ttttccatca gcgtttccgc atcgcttgtt tttcctgctt
     1441 ttacttgatg gaaaaagttg tcaaaactga acgtgctgtt gctgtggtaa agactattga
     1501 tgaaaggtgt aacttcaagc tggagaccat tctcatctgt caatttataa tcgatcagga
     1561 attgctggaa gctttctaaa tggatataaa tcacattttt tcctttagcg agcccgaaca
     1621 aatcatcatt tggtgctgca taatgttctt ttacataatt ttctacttct ttcatatcat
     1681 tgggacttgc ttctgcccgc acttggtttg tctggtaagt ttgaacaccg tcataaacca
     1741 tgaaagcatt caagccaaga tatttgacca aataatcacg agagaatgtt cgagtcagca
     1801 attctggacg atcagcttcc gccataaaaa gatttcctga gaaaatcata actgctaatg
     1861 tagagatcgc aaaagccatt cttgctcgga tcgggcgagg gtccaacttg attttttttg
     1921 caatcagcag aacagtcaag ataataaaat cgatccaata gatcacatca tatgggcgaa
     1981 acagtcgaat ggcactttct cccagaccac tcgctacttt cccagcacct aaaatcgtgt
     2041 tgatcgtaat gaagtccgtg aattctcgat aataagtgac attcgaaaaa acaagcaatg
     2101 tcaaaagaaa ataaatgacc atcatcgtga tataagcaac tctagggcgt ctgatataaa
     2161 gagcaattcc aagtaataac aatgtagttg caatagggtt gataaacaag ataaagtatt
     2221 gaatgccact ttcgattccg agatggaaat caactaaata ggcaaaaatc gttttcaacc
     2281 aaagtaaaac aactagtaaa gaaaaaaatc cgagcctctt attcaaaaag gcaggtgttt
     2341 ttatacattt caagataatc ttcctttctg cacttcttaa tcacagaagt gtcatgaaac
     2401 catgttacac agctcactta ttctcattta ttttactagt atgacaatat agtgtcaatt
     2461 gaaaccagaa gtttatgctg tttcattaag gttttcttta agcttcatga aaaaatgagc
     2521 agtaagacaa gtcctatgtt ttttgttata cttgggaaga gaaagctaaa gcaggtgaag
     2581 gaatgaaagt aaaagtaaaa aaaagagcaa gcaatcgatt gaaaaaagga tatccactga
     2641 tccaaaaaga agaccttgct catccccaaa aagaaaccac gcaatgggtg gaattagttg
     2701 attcgcaaaa ccaatttatc gcaaaaggat atctaggaaa gcagaataag ggaatcggtt
     2761 gggtattgag ccagcagaac atcccatttg atcaatcttt ttttgaagaa ttttttgtga
     2821 agtcaaaaga aaaaagaact ttcttttttt cagatgattc tactaccgct tttcgtatat
     2881 tcaatggaga aggagacggg atcggcggtc tgattgttga ttattatgca ggttatgctg
     2941 ttttttcttg gtataacgaa acgttatatg cacatcgtga attattgatt caagcttttc
     3001 gtgaaatcta cccggaaatc gtaggaggct atgagaaaat tcgatttgaa tcagtaaatc
     3061 tgcctgagag ccagttcatt tacggaaaag aggcaccgga acccttgatc gtaaaagaaa
     3121 acggagtaaa atttgccgtt tatttaaatg aaggattaat gactgggatc tttttagacc
     3181 aaaaagaagt acggggatca ttagcagaag gtttcgcttc tgggaaaaca gttttgaaca
     3241 tgtttagtta taccggcgcc ttttctgttg ctgccgcaat gggaggggca atcgaaacaa
     3301 caagtgtcga tctagcaaaa cgcagtttaa aaaaaacaca ggaacagttt gaagtgaatg
     3361 gtttggatgt cgatcagcaa aaaatcattg ttatggacgt attcgagtat ttcaaatacg
     3421 ccaaaagaaa acaattttta tatgacatga ttgtattgga tccaccaagt tttgccagaa
     3481 ataagaaaaa agtctttcgc gtagcaaaag attatgggga actggtcaag gatagtctgg
     3541 atattttagc agaagacggg atacttattg cgtctacaaa tgctgcgaat gtctcgatag
     3601 aacaattcca aacaatgatt gaagaggaat tccatgccaa aaatgtttct tttgaacaac
     3661 ttgctttata tcgtctgcca aaagatttcc agacgacgga taccttttta gaaggcaatt
     3721 atctgaaagt gtttgtttat caaattaaaa ataaccatta gaaggagcga tgcagaatgg
     3781 tttgggtaaa gggattgaat ataggaagcg gtagaccaaa gatttgtgcg cctcttatcg
     3841 gggccgtccg agaggaagta ttacgtgaag cagttcttgc acgtgaagca ggggccgatt
     3901 tagtcgaatg gcgagtcgat cattatcgtg aagttttttc ttttgatgaa gtagcagaaa
     3961 cattggttct tatccatgag atgcttgatg gactgccgtt attatttacg tttcgtacct
     4021 tagctgaagg cggagaacaa gacatatcga tccgtcagta tcgcgacctt tatgaacatg
     4081 tgatacacac aggattagta gatatggtcg atatcgagtt gttcaaagta gaaagtctag
     4141 gaaaaagact gatagaggaa atcaagtctt taaacattcc tttaattatc agtagtcatg
     4201 attttaaaga aacaccagca gaccctgtct tgctttaccg attgaatgtt atggaacatt
     4261 ttggagccga tatcggaaaa ctggcagtaa ctcccaataa tgaacgagat gttttgcggt
     4321 tgatggagtt gactagacgg gcaaatgctt ttgtctccat gcctattatt acgatgtcca
     4381 tgggaaatct tggtaaaatt tctcgtctgg ctggcggaac gacgggttcc gttatgacat
     4441 ttggttcctt aagcagcgat tctacttctg ctccaggaca attaccggtc aaagcattga
     4501 agcaaattat tcaattatta aatgaaccgt tgtagaacaa gtttgaggtg atattttttg
     4561 gcaaagaaaa aaatagtaaa aacgaatgca atccgtatgg tcgaacaaaa aaagattccc
     4621 tatacagagc acgaatatga gtgggacgaa agccacctaa gtgcgtcatc ggtcgctgaa
     4681 cagcttccag aaagccaatc tcatatattt aaaacactgg ttgctgtggg caatgtgacg
     4741 gggcctcttg ttgctgtgat accgggagaa gccgaattga atctaaaaaa attagcaaaa
     4801 gtttcaggga ataaaaaagt agaaatgctc catttaaaag atttggaagc aacgaccgga
     4861 tacatacgtg gtggctgctc gcctataggt atgaaaaaac tatttcctac ttatttagat
     4921 caaattgcag aaagctatga acaaatcatt gtctcggctg gcagaagagg gctacagatg
     4981 gagttagcac ctcaagatat ctgtgcatta acgagcggac aatttgcaga tataaaacaa
     5041 taaatcatac gtataaaaga aagaaggagg ccgggatgtt gtttgtccag tctccttctt
     5101 tcttttttat acattatgct ctttgcccgg cattcattcc tgctacatgc cctgtagcga
     5161 aagccgctgt gatattaaag ccgccagtat aaccattgac atccagtaat tctcccgcaa
     5221 aatacagacc atcaatgatt ttactctcca atgttttagg ataaatttcc tttagagaaa
     5281 cccctccgcc agttacaaat gatttctcaa taggaaaagt tttgttgatt gatagcttaa
     5341 actctttgca tagttgaaca aaatcttgaa tttgtttttg agaggcttgt tgtcctgtca
     5401 gatgatccat ttcaagccgc tcaagaaaga aaactaacaa tcgctcaggc aatataccat
     5461 gccaagcatt gaccaagttc tttttggttg ctttactttt ttctgtcaat atatgaataa
     5521 gctcttgttt ggtttgtgta gggaaacaat ccagagaaag tgtcactggt tctcccgttt
     5581 tctctaattc tttattgaca aaacttgaac aacgcagtgc agccggacca gaaataccga
     5641 aatgggtgaa aagtaaatcc atcgtatgtt cagtcaatac acgacctttt ttatttaaaa
     5701 cgcgtaaagt gatgtcttgt aaagaaagcc cttgaagtgt tttctcctga atatacggtt
     5761 catccgatat caagggggac tctgtcgcat aaagaggcgt caccgtgtga cccactcgct
     5821 tcacgatctt gtacccatct ccagtagaac cagtagatgg ataagtccgt ccaccagttg
     5881 ttaagataat ggcaggagca tggaactctt cgaattccgt gcgtataccg taaattttat
     5941 gttctttgtg gatcaatttc tctactcgag tagagaatag aagcgtcaca tctagttctt
     6001 tcagacgatt gagcaatgct tcgataatcg tttttgattt atttgttaca ggaaacatcc
     6061 gtccatgatc ctcttctttt aaatgtacgc cttgtgattc aaagaagttc atgatatcga
     6121 aattattcca ttgcgcaaac gtactatata aaaactttcc atttccagga atatgtgcaa
     6181 tcaaatcatc aacaggacga ttgttcgtaa cattgcaccg accaccacct gtcatcagta
     6241 gtttttttcc tgcttttttg tttttttcga tcaagagtac gcgtgcgcct tgttcagctg
     6301 cactgatcgc tgccatcatc ccgcttgttc cagcgccgac tacgatcaca tcatacagtt
     6361 tttccattcc ctcacctcaa ggaaagtgta gcatatctga agaaagaaaa cagaaaaaat
     6421 gttttgagcc aaaagagaaa atgagctaaa atgagggaag ataagcaaag gagcgtggaa
     6481 cgatgacaaa cgcaacagaa tatgttcaag caattcaaga aaagctacat caaaaggatc
     6541 aagggcaaga agaattttta caagcaatcg atgaattcat gcccacggtt atgtcttttt
     6601 tggacactca tcccgaatat atcgaaaaga atatcttaga acttttaacg gaaccagaaa
     6661 gagttattca atttcgagtg ccttggcaag atgatgaagg aaactggcga gtaaatagag
     6721 ggtaccgtat ccaatacaac tcagctatcg gcccttataa aggtggattg cgcttccatc
     6781 ctagtgtcaa tctaagtatc ttgaaatttt tggcttttga acagattttc aagaatagtt
     6841 taaccggtct gccaatcggt ggaggaaaag gcggaagtga cttcgatcct aaaggaaaat
     6901 ctgataatga gatcatgcgt ttttgtcaaa gtttcatgct ggaattgtca aaacatatcg
     6961 gcccatcgat tgatgttcct gcgggagata tcggtgtagg agctagagaa atcggctatt
     7021 tgttcggcga atataaacga ttgaaaaaat atgatactgg tgtgttgaca ggtaaacctt
     7081 tggatttttg gggaagtaag atacgtacgg aagctactgg ttatggatta gtgtattatg
     7141 tcaaacatct attgaacgaa gaaaaagatt catttaatca aaaaacagtt tttgtttccg
     7201 gaagcgggaa tgtagccatt tatgcaatcg aaaaagtgca agagctaggc gggaaagttg
     7261 ttacatgttc agattctagc gggtacatct atgatccaga aggaatagat gtgaaactat
     7321 tgaaagaagt caaagaaatc aatagacaac gtttgacgga atacgcaaac caacgtcctt
     7381 ctgctattta tcatgctgga gaatctgttt ggacattgaa agaaaaagcg gatatcgctt
     7441 tgccatgtgc tactcaaaat gaaatagacg aaagcttggc cgaaatctta gtagaaaatg
     7501 ggatcaaaat cgtagcagaa ggcgctaata tgccatgtac agtaaaagct gtttctgttc
     7561 ttcatgaagc aggcgtgtgg tattgtccag gaaaagctgc taatgctgga ggagtagcag
     7621 tatctgcttt agaaatgagt cagaacgccc agcgattgac ttgggaaaga gagcaagtgg
     7681 atgagcagct ggatcaaatc atggaaacga tttaccatac gtgtcgccaa acagcagccg
     7741 agtacggcga cgaaaaaaat ctgcttcttg gagcaaatgt tgctggattt gaaaaagttg
     7801 ccaaagcaat ggcagcagaa ggtttagttt gaaaaatgaa actttcttgg aaatattgtc
     7861 agcattttgt ttatttgctt gaaaaataat aaaaaaaaac gtaaagtatt acctgtagag
     7921 taaacctata aggaggagaa caaatggcac acattcattt tgactattca aaagtagcac
     7981 cgtttgtcaa cgaacaagag ttagggtaca tgcaaagcca agtaacagca gcccacaacg
     8041 aattacggga aggtactggc gcaggaagcg acttccgcgg ctggatcgat ttaccaacga
     8101 actatgacaa agaagaattt gcacgtatca aagaagcagc taaaaaaatc caatctgatt
     8161 ctgaagtatt agttgttatc ggtatcggtg gttcttattt aggcgctcgt gcagcgatcg
     8221 acttcttgaa tcacacattc ttcaatttat tagatagcaa cacaagaaaa gcaccacaaa
     8281 tcttctttgc cggaaattca atcagctcaa catatattgc tgacttgatc gaagtaatcg
     8341 gtgatcgcga cttttcagta aacgttattt caaaatctgg aacaactact gaaccagcga
     8401 ttgcgttccg tgtattcaaa gagcttcttg ttaagaaata tggacaagaa gaagcaaaca
     8461 aacgtatcta cgctacaaca gataaagcaa aaggtgcagt taaagtagaa gcagatgcag
     8521 aaggttggga aactttcgtg attcctgatg acatcggcgg acgtttctct gtattaactg
     8581 ctgttggttt gttaccaatc gctgttagtg gtgcagatat cgatgcattg atgcaaggtg
     8641 ccgctgatgc aagcaaagca tactcaagcg acaaactaga agaaaacgaa gcttaccaat
     8701 acgcagcaat gcgcaacatt ctttaccgta aaggcaaagt aactgagttg ttgatcaact
     8761 atgaaccagg aatgcaatac ttctcagaat ggtggaaaca attgtatggt gaatctgaag
     8821 gaaaagacca aaaaggcatc tatccttcaa gcgcaaactt ctcaactgat ttgcactctt
     8881 taggacaatt catccaagaa ggacgtcgca atatttttga aacagttgtg aaggttgaaa
     8941 aacctcgtaa atcaatcaca attcctgaac aagcagaaga tttagacggt ctaggctact
     9001 tgcaaggaaa agaagtggac ttcgtaaata caaaagcatt tgaaggaaca ttgcttgctc
     9061 atacagacgg tgacgtgcct aacttgctag tcaaaattcc agaaatggat gcttatacat
     9121 tgggttatac aatgtacttc tttgaaattg ctgtaggtat ttcaggttac ttgaatggaa
     9181 tcaacccatt cgaccaacca ggtgtagaag cttacaagaa aaacatgttc gcacttctag
     9241 gcaaaccggg ctttgaagat cttgctaaag aattgaacga acgcctgtaa gataagggtt
     9301 tttcgctaga tttaaataat cagtgataga aacatgttcg ctttaaaatg aaaactggta
     9361 cccatttagg tactcaaaca tactgcatcg tttattcaga ataaggggct gtgacaagac
     9421 gtttgtcaca gtcccttgtt tgtatagaaa tcgattgatt gccttgctct ttagtttgtt
     9481 ttaggacttt aagaacagtg aggctgactt cgaaggttaa ttctgttttt ttgttttcta
     9541 gaatcagtaa gcaaaaaagc actcacttcg agtgcttttt tgcgatatat tcttagctta
     9601 agataggcct aatttttctt ttacagatga gggtacctta tcgctggtaa ttactttttt
     9661 ctctatgatc cgcttttcgc ttgctttcaa ctgcaagatc gttccttcgg tgtaagcaga
     9721 accattggca gagtcaccag aagtaatgac atcgaaatct aactgtcttt tttcaccgtt
     9781 ttcagcataa gcatctacgt tgtatttaaa acctcttcca attacttccc cattatctgc
     9841 tttgatatca gtttctgcag gcattggagc tttgactacc gcataataat ctttacctac
     9901 ataagtagac tgatagtagt cccaaccttt ccatcctata aaagcaatca atatgataac
     9961 gatcaaccct aaaatttttt tcatgaaaga tctccttttt tatttttatt atattaaata
    10021 ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt cataaaaaac
    10081 gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag agagacaatt
    10141 tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt acaaacgtct
    10201 tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc cttatctaga
    10261 ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt tattttcgtt
    10321 ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc ggtattgcat
    10381 atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta gttcttgaaa
    10441 gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt tgatgcaatt
    10501 aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct attttctttt
    10561 aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat ataaatcata
    10621 tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga acaacatagg
    10681 aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata gttatctaaa
    10741 aagcagaaag atactcaaaa taaaaaataa aaaaatgaat aaaaacgttt tcaaatacaa
    10801 ggtaatttgt tattataaaa taaagaaaaa acaaaataaa aataaagaaa agagggaatg
    10861 aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga gtatctcatg
    10921 aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat gttgaaagtt
    10981 atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc gtaaaattct
    11041 gtgatattag cgcacctact gaattggttt cttaaacagt atttttgaga attcgcaaga
    11101 actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa aaagaaaaag
    11161 ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta ttttagctat
    11221 tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg tcaaacaaaa
    11281 aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac ttttttcatt
    11341 aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg aataagtgat
    11401 ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg ttatgatacc
    11461 atccacacca taaaacatca tgcgactcat ggcatctgaa tcgttgattg tccatacata
    11521 tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg tactatattc
    11581 cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag gtaagatgta
    11641 gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt acgtcaaact
    11701 ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa atcgttcgac
    11761 aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt ggtttagctt
    11821 atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc cgttttcagt
    11881 aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat ttacccctgt
    11941 taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg tttcctgaac
    12001 atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg cttctaaagt
    12061 attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg gttttgtctg
    12121 aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga aaaaaactgc
    12181 aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg cttgtgggat
    12241 aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa caatgatata
    12301 aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta aaagcgtcat
    12361 agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca tactttgtgc
    12421 caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc caatcacaat
    12481 aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt cccgaatggc
    12541 tgctcgaaaa gaacgatctc gtagaatcat ttccggtaaa gcaaaaatca gtcgaatccc
    12601 aatatagcca aggaaaatat aaacgagtaa aaaggaactg acgatgatcc aacgattagc
    12661 aaaaataaaa tccataatga aagcggggat ttttattttt gacagtagat cagaattaaa
    12721 acttaatccg ctgatcggta gaatcaataa aaaataagca aggaaaaaca aaaaagtaat
    12781 gggtctcact ttcttcagct gaagtatcgt caagtgtaag agttgcttca aagagattgg
    12841 ttctttcttt ttaataaaaa aaacactcat caaaagaaaa gtaaattcga aataaactaa
    12901 cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga accccaggat gttgagaaaa
    12961 aattgaagga atattgtcat aagacaaata gtcaattgcc cctcggtgta aaataaaacg
    13021 ggtcgtactg ctcaataagg gtaaacaaat aaataaaata aagccatgca tcagcaagac
    13081 atcacggaaa taagcttgtg ttccttttaa aaagtcccaa gcattaccta agctacgttt
    13141 caaataattc atcctatcac ctgctcttca cattgtagcg caaatctaat gaaaaaggaa
    13201 agggtgcagt gtgttttgca gaccctttc
//
LOCUS       DACDIH010000069        13058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDIH010000069 DACDIH010000000
VERSION     DACDIH010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.069"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(126..1457)
                     /locus_tag="IV281_002164"
     CDS             complement(126..1457)
                     /locus_tag="IV281_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8781139.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(1423..2901)
                     /locus_tag="IV281_002165"
     CDS             complement(1423..2901)
                     /locus_tag="IV281_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8781140.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(3206..5041)
                     /gene="tdc"
                     /locus_tag="IV281_002166"
     CDS             complement(3206..5041)
                     /gene="tdc"
                     /locus_tag="IV281_002166"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8781141.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            5511..6938
                     /locus_tag="IV281_002167"
     CDS             5511..6938
                     /locus_tag="IV281_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8781142.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(7030..7395)
                     /locus_tag="IV281_002168"
     CDS             complement(7030..7395)
                     /locus_tag="IV281_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8781143.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(7392..7757)
                     /locus_tag="IV281_002169"
     CDS             complement(7392..7757)
                     /locus_tag="IV281_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8781144.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(7949..8197)
                     /locus_tag="IV281_002170"
     CDS             complement(7949..8197)
                     /locus_tag="IV281_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781145.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            8196..8324
                     /locus_tag="IV281_002171"
     CDS             8196..8324
                     /locus_tag="IV281_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP8781146.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            8381..10075
                     /locus_tag="IV281_002172"
     CDS             8381..10075
                     /locus_tag="IV281_002172"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP8781147.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            10142..10348
                     /locus_tag="IV281_002173"
     CDS             10142..10348
                     /locus_tag="IV281_002173"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781148.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            10726..11859
                     /locus_tag="IV281_002174"
     CDS             10726..11859
                     /locus_tag="IV281_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP8781149.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            11890..12210
                     /locus_tag="IV281_002175"
     CDS             11890..12210
                     /locus_tag="IV281_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781150.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(12239..12703)
                     /locus_tag="IV281_002176"
     CDS             complement(12239..12703)
                     /locus_tag="IV281_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8781151.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(12835..>13058)
                     /locus_tag="IV281_002177"
     CDS             complement(12835..>13058)
                     /locus_tag="IV281_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8781152.1"
                     /translation="VSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAA
                     VNLVLSILIYLPFVKVATIQERRKEALDA"
BASE COUNT         4261 a         2614 c         2253 g         3930 t
ORIGIN      
        1 gtaccagttc atcctatccc ctcctgttta attgatactg ttattatacg cagggaagtt
       61 ttttcaggcg tatcgattat ttcagggggg tctgaaataa ttcgttttgc aaaatgtatc
      121 tgggcctaac tgatatttct attgcttatt cttttatata caaaatttaa gaatacaaac
      181 cagataacca ataaaaaagg aataaagcgt gtcgtgcttt cgaataacaa gctgaaagca
      241 attgaagtaa aaaacaccaa caaagcaaga tctttatatc tttgtgtagt gttccgtttc
      301 ttttctttaa taaattgaac atgtgcacac aagatcgaag accacacaaa tagaaaacaa
      361 ctcgttgtca tgcctgccaa aagctgaaaa aacaatcgac tatcagctac aaaaaaatgt
      421 agacaaagac cggcaaaaaa taacattcct gtaattaaaa taccgtgtaa aggtacgcct
      481 ttcttagaaa gcgcacaaaa ccaatggggc aaactttttt cttttgctgt ttgatataaa
      541 atcctgctag tactgtagac tgcactattt gctgaggaca aagaagcaac caaaatcatt
      601 agattgacaa ttgctgctcc gccttggcaa tggatcactt gcagcatagt cactaaagga
      661 ctttctttta aatctaaaca atgccatgga acaacaaata aaatcgacaa aatcgttcct
      721 atatagaaaa agagaatacg ccaaggcaga cagttgattg cttttttcaa attcttttgc
      781 ggatcacttg tctcgccaac ggttaagccg atcatttcaa ttccagcaaa actgaataaa
      841 accataggaa atgagactac cattccagca aagccaaacg gtagtaattc atcgatctgt
      901 ccaaaatttt ctttgattcc gatagactgg gtatgaaaat ctgagacact caatcctata
      961 cccaataaga taaaaaggaa aatccccaag atcttgatga ttgagaaaaa agattccaat
     1021 tcaccaaacc acttgactgc aacaaggttg actgccatta agatcattag tgcgctacct
     1081 ccgcttatca aagctggtac atgtacaaac cagaaatgga tatacaatcc tattgctgtt
     1141 aattccgcaa gtccagctcc aagccagcaa agccaataag tccaattgac aaaaaatgcc
     1201 cactttttgc ctaaataata gtcaacgaaa tcactgatag agttaaatgt actatttgct
     1261 aaaaataaat cacccatggc ccgcatcaaa ccataaatag ccaaaccgca tatcaaataa
     1321 acgagaataa cagctggccc tgctgatgca attgcctttg ctgaacctaa aaataatcct
     1381 gttccaattg cgccacctaa tgcgattaac tggatatttc gattagttag agtccgtttc
     1441 attgccggtt tttccatcaa gttcaccttt ttctttttca agcagcatgg cgacttcctg
     1501 ttcccaagaa ggtttcttta ttttttcaat acagagtggt actacaaaca aaatgacact
     1561 gatggctgtg actgcaatta gattgatcca tgttccagct ttcatcgtca gtccgacaga
     1621 aaatccgatt gccgcaaata aaacaataac gacagtccaa actccaagat tgctcttttt
     1681 ctcttttttt ccaattctaa atggtcgatt caactttgca tgtgtataac gcaatctcaa
     1741 aactgcgatc cccatgatga catagacgat acagtagata acagtagttg aatttacgag
     1801 catcaaaaat gcttgattga cgccaggaat cagcaaataa aaaagtgcaa agattgagat
     1861 aatgacagac tgagtcaaaa taacggattt ggatacgtca aattgattcg tcttccaaaa
     1921 attgaatttt ggtggatacg caccttttct agcactttct gtcactgtct ttgctggtcc
     1981 tgaggcccaa gcagataatt ggactgctac accaatgaag acaagtaaac taaacagatt
     2041 cacaataaga tgtggcaaac caagaatttg gcaataaatc gaaatagatt gcgcgatatt
     2101 attcaattcc atttgtcctt ttggtacgac atttgctaca actaaagcat tcaaaatatt
     2161 gacgaaaaat ataaaaatta acgcggcaaa tactccgcga atatacgttt taactggatt
     2221 ttccaatcga gtgatataca cagaggacat ttcgatacca gtaaaaataa acatgatcgg
     2281 cgcaaaataa acaaatgagg atgcagtcgt agtatctggt attaatttgt cccatgaaaa
     2341 actgcctaat gttccggttg gtacgatccc aactttgatc cacgcagcaa accctaataa
     2401 gagcatcatc gtcaaaggca cgtacaatcc taaccaaaca ccgacttttc cgccgatttt
     2461 tgccatatca aatttcaaat tcaaaaaagt gatgatccaa taaacgacta aaatgacaac
     2521 taatgtaaat ttgctgttca aaccaagcgg cacattatcg ataatattac caaacaacgg
     2581 agcgagtgct gaagcgacca tcaccatccc aggaaacatt tggacccata acagccatga
     2641 gacaacgaac ccccattttt ttcctagagc attcgatacc caaagttctg gaccaccttt
     2701 ttggggaaac attccagcaa attcaccaga gatcaatgag attggaaaag cgtacaataa
     2761 tactgcaacc agcatgtaaa aaatcatcgt ccagctcgtt gctgctacat ctggaatatt
     2821 tcgtacactt gctacaaggg cgcaggtcat tccgataaag gtcatggtcg tgatttgctt
     2881 tgatgaacca gtatctttca tctttattcc ttctttcaat agcgacatat gtttttgttt
     2941 tcttttattt ttgttcaaga acagaaatga caaggtttca tcgattcagt acaaaaccca
     3001 gtatcaccga aacatccatt gaacgatctt agtgaactgg gtttgataca tgaaacgaac
     3061 gatccaactt atagaacgtt ttataaaaaa gaatatcttg gcgaggaata tatgaaaaac
     3121 agggggaaat acaattgttt tgatgaagca aataattgtc tgttctccat atgtctcttg
     3181 ttattctttc acacaaaaat cagttctatt ggatgatttc gtttagtttc ttttccattg
     3241 ctttggcgat tttttttgta taaaaattga aaattcgatc atcattcaaa taaggagtca
     3301 tgatagccgc acgtagcaga gtgattttct gttctttttt ccattcttct ttcgttagac
     3361 ccattctttc aacaaaacga atcggagaat caccgtaatc ttcttgtgta aatgtagtat
     3421 gagaagtaat aaaccgttca ccataaacat cgccatccat ataagaagac cgatcgaaca
     3481 ttttttcatt taattcattg atagcgttca aatctgtaca gccttgttct ttgaataccc
     3541 aattgaccat attaaaatca ggatgattca atggatagac ttcgattgtt tttcctttaa
     3601 cagtaaaagt cagatgatcg agaaattctc tgaatctttg agctgcctcg attgaagccc
     3661 caatcaattg accatatccg gtcacattca atggcaatac acgatgtgct gtccagactg
     3721 cggcggcggt tgctccagct tttgatcctt ccaaaatata agctcctaac atatctggag
     3781 cctttactga tttttcaaac acatatggcg cgaaataaga aatgatattt ctcatatttt
     3841 tatgtttgat tacgattcct ccagcagcat aagggacata gcccatctta tgaggatcta
     3901 tcgtgacaga atccgcttct gaaatagctc tgaacccttc atatacctct ttgtcaatcg
     3961 ttacgcagtg gtggaaaaca tgatgttctt caaagaattc agctaaggaa gcatacggca
     4021 caaattctcc tgcttcgttt agaaacagtg aacgcgcata gccgccatat gcggcatcaa
     4081 catgtaaata gaaatagatc ccttcatctt ttaacctttc tcttaattgg acgattttat
     4141 ctacactgtc tacttgacct tcttctgtcg ttcctacgac agcaaccaca cctagaattg
     4201 ggattttttg tccagctaat tcacgaattg ttttttccaa agcattaata tccattcgat
     4261 aatcctcttg aactgggata gcaaccatct gatctaagcc gactccgcaa atatctaacg
     4321 ccttcatcca agaatagtgt tttgtttgag ggaccagcca tttccctaat ctttggatat
     4381 tttttccgct acgggaagaa gctgctttga cctcatccat ttcttcgtcc gtaaagcttt
     4441 cggtcatttc caaaatttct tcaacagata aattcaaaag ttcccactca gacatttttt
     4501 tcactttttc aggcagcact tccttgacag ctaaaggaat cgatttgatg caacgggcgt
     4561 accataatcc ttcaagatta gcaatagaac catctgctgc aatgtgtccc catccgtctg
     4621 tcatattgaa taatgaagca aaatcctgtc ctacttctgc ttccatttgc gaagttgcca
     4681 tagaagattc caatgcaaca ttatttgggt tccacagcat cgcatagttg taagctaata
     4741 aagcaggcat caatgtttca gcattcattt gtccccagta acggcctgct gaatgccatg
     4801 ggatcgatcc tgcgcgtaat cgctgactga cttcatccaa gacctctatc atatggtcgc
     4861 gggtggctag ataatctgga gtgagcttat ctccttcgct aattgcaggc atatctgaag
     4921 gaatataatt ttctctccat ccaagatgtt catctaccat cttgttcaac aacattttat
     4981 aaactggtcc attttcagct ttgtcgccta taaagacggc cttgatatcc atatctttca
     5041 tttttcttcc ctccatattt tgttggtcaa tgtcttgcag atacatgtgt ttatctgtaa
     5101 cacctcttca ctatagcgcc aaaaatgaga attttttttt tataacatca aatccacgta
     5161 gatttttaca aacaaaaata aataaagtcg gatttttctg ataattcaac gcaaaaaagc
     5221 gaacaatttt tcaataaatc agtcttctaa aatagataac ctaaaaaaag cgaacaaaca
     5281 aataaaatga ctgtccttca ttcactaaac atatttttat ttttttatat tgttaaaaat
     5341 tcgcaattta ttttttaata gaattatctg ttttccaaat tttataacat aaaaaacctt
     5401 ttttaacggc atttatatgc tttttcagaa attaactgat gatttcaaaa agaaaacatg
     5461 tcataatatt tcccatattg tgcaaaaaaa tacaaggcgg aggctctact atgattgaag
     5521 aatatattga gaaaaatatt ttaagacagc tgtttttatg tgggcaattc tatgtaaata
     5581 aagaagtcaa tctagaaaag ctctccaatc tcctgcatgt ttgcaaaacc actttactca
     5641 atgatatcaa taacataaaa aaagaatttg aagaacagat cgcttataca catcgggaaa
     5701 aggaccgtta cactttatat ttttcagaac atatacctcg atgcaagatc atgcagcaat
     5761 tgtctcaaaa ttcgttgttt ttaaaaactt gtctgcttta tttggaagaa gatgaacctg
     5821 attatcttca attaactgaa tgtgaattca tctctgtcag caaagcttat tcattaaaaa
     5881 aacaagtatt agcttatttt aatgactgcg ggatcgaaat cgaccgctac tcccctcgat
     5941 tcactgaaat ggaaagacgt ctgctgcttc ttaatgtgtc gtatcgttta ggcggtttta
     6001 attcttggga gctacctgaa tcgttttttg aacgtgccga tcgtttcatt gaatccgtaa
     6061 cggaaaatag tggcagattt tatgataaag aaaataaaga aatcttatcg attggttttg
     6121 ctatcagttt cctacgccag caagtgtgtg ctgtgacaat agattcaaaa tttatcgaag
     6181 aaatcaaaaa acgtcctatt tacaattatg tcgaatcagc atgggaaaac actgatttcc
     6241 aaacctatta taaaaaagaa gaattcgcct ttattttgac actattcaat ctttgtaatt
     6301 atggtttcca ttcttatcag ttgattgcgg aagactttca acagctacac caagtgttta
     6361 ttgacaatac tcccgaaata aaagaactag ttgctacctt tgaatctcac ttcaatcaag
     6421 aattattcgg aaaccaaccg ttcgaacgtg cgctgattca tctgatgcgt tcggcttggg
     6481 ataactatca attattcatg ccagaaaaat tttatctttt aaatgaggaa cagacaaatt
     6541 tatataaaga agtccagaca attttttcca gctggtccag ccagttacct tatgatctac
     6601 ggttgaatcc taattgcatg cgggcttttg tgatcgaatt gagcggaatt ctccgtctga
     6661 caaaagaaca tctgactatc tatatcgtca caaattcgga tgttcattat ctgatttatc
     6721 gggaagcctt ggaagctgta actactttcg attttcaagt tgcaccaaca atttattcaa
     6781 gtatatctga tataaagaag tatgcgcagc agtcctcaaa ccgggtatta tgtgaacgta
     6841 cattatatac accagatgcg gtccaatatg aaaatattat tcctatttcg atcaatacga
     6901 tcgatcgagc tatcatttca gctgtgcaaa acaaataagg ggctgtgaca aaaatcatgt
     6961 cacagcctct tatttgttga agatgcccat ttctgtaaca atctcttgat acacgtcagg
     7021 ctcacaacct cacttatttt ggacatctac gggtccgatc tctttattta aaaaataaga
     7081 aatgatggtt cggataacta ccaacgccgc tagttggaaa atatcctgca aggtaggttt
     7141 gataatcgat tcgatgatcc cagctgaaac aagaatttcc aaacttagaa gtatatagcc
     7201 tcccaacatc tttttaatag cattgttctc gctgactcgg tcgctttgca cttttgtatg
     7261 gacaaataaa tatttcaaaa aatccttgat tgatagacaa acaccccaaa ccaataaaca
     7321 gatagcaaag atatcaagtc ccagtacaag tttttcgaaa tatggaagaa gaaactgcat
     7381 caactctgtc gtcatcctac ctctcctctt ctttgttttt ctttgtcttc tatttcctga
     7441 ttcaagaaat aagaaatgac tgttcgaatg ataataataa gtgcaagctt caaaatatct
     7501 gtccaagtcg gcttgataac tgactcgatg atatcagcaa cgactagtac ttccaagctt
     7561 aacaaaatat aacttgctag cagttttctt gtatctgcat tacgagtatt acgttctgca
     7621 tagtttttct tgacatgaga ccaatcaaaa agtttgaata gaacaagaac gatcccgaca
     7681 attaagatac cgatagaaca aatatttaaa atagcaatga tccattctag aaattcaatt
     7741 aaatttgagc gaaacatata cgctacctac tttcttttaa tttgttccta ttcttttatt
     7801 gtaaaagaaa gttaccttgt tcgtctattt aaaagttgtt ctaatgataa cggttcgctt
     7861 aaatttttct ttttcactct cccatttttc tccctccatc cataaatcgt ggaagtatcc
     7921 aaaacaacgt agcaagttaa aataaggctt agtccgtcat cagcttttca aagaagcact
     7981 gtctcggtgc tttttttgta cccaaatttc taaaaataga accatacacc tcctcaaatc
     8041 ttttcttctt acctacccta tttttattta caggaaaatc agacacttct tcttttttgt
     8101 caaaatttcc tttttcttct cgtcctatca tttggcattc taataaaaac gaactatcct
     8161 tggatataga aagagaaaac aaggaggcgt ttttcatgag taaaaaatta atcaaagtcg
     8221 gcatcggttt aggtttgctt gcccttggcg ctgcatatct aggaaaaaag acaggactat
     8281 tcgaagatga cagtcattta tatgatgaat tcgaatcaat ctagcatcct atttagttga
     8341 atcactacgg acatacttgg aagacattgg aggaatcaca atgagaaaaa gtaaagcaat
     8401 aatgattggt gccggtattg ccaatatggc agcggctgtt tatttgattc aagaaggcaa
     8461 atggaaaggg gatcagatca ccttttattc attggatgat cacggttcaa atgatggtgc
     8521 accgaccgac actgtagtgg atgaatattg gaataaaaac catccattag aaaaccaaaa
     8581 aggctatatt gctcgaggcg gtcgcatgct taattaccgt acctatgtag atttgatgga
     8641 tctgttaagt cgaatacctt ctgttactga gccagggatg actgctgaag aagatacgag
     8701 agattttgat gcaaaacacc gaacctttga caaagcacgt ttgatggaag gtggcaaagg
     8761 aatcattgat ggcggaaaac tgggattcaa taataaagac cgtattcttc tgactaaact
     8821 gattgctatg cctgattcag aagaagaaaa actagacaat atcactatcg aagaatattt
     8881 caaagatgat ccacactttt ttgaaacaaa cttctggttt atgtgggaaa caacatttgc
     8941 tttcagaaca cgtagttctg ctcaagaatt gcgtcgctat atgcatcaaa tgatttatga
     9001 attcactcaa atcgaacatc tagtgggcgt caatcgaaca cgttacaatc aatacgaaag
     9061 tattatgctt cctttgatcg agtacttgaa agaacaagga tgcaaaatca ttttgaaccg
     9121 ccgtgtcact gactgggaat tcaaagaaac accgatgcaa gatgaaatca ctgtgactgg
     9181 tttgaaaatt accaatacac tgactcaaga agaagaatac gttgtggtcg atgaagatac
     9241 agcagttatt ttcactaatg ggtccattac agattcagct actttaggag attacgaaac
     9301 accagctgta gaaaatatgg attatggtgc agcttctagt ctctggaaaa aagccagtga
     9361 acgtttctac aacttgggaa atcctgacaa attctttgct gatcgagatg caagcgaatg
     9421 ggtcagcttt accctgacga cgaaaaacca tttgttgttg aatgaaatca cccgtatcac
     9481 gactcaagaa ccaggaaatg ccttgaattc atttatttca accacaccga tcacgccgtt
     9541 aggccaaaaa gatgtcaata tgtctattgt cgtgcatcat caaccgcatt ttacgtctca
     9601 gaaaccaaat gaaacagtta tttggggtta tttcctttat ccaagacgac gtggcgaatt
     9661 tgtggataaa cagtatatta aaatgaatgg aaaagaaatg ctagaagaac tgattggaca
     9721 actttcaaaa gtagatcctg gaccagtgaa tattcgtgaa aaagaaacag aaattttcga
     9781 cagcgtaata aataatatcc ctgtctatat gccttatgct tccgcgttgt tcaataatcg
     9841 tgccaaaagc gatcgaccaa aagtcattcc taaacattcg accaatctgg cctttacagg
     9901 agaatttgtc gagcagcctt atcagatgat ctttactgaa caaagtgctg tacgttctgg
     9961 agaaatcgct gctttccatt ttgcgggtat tcctatgtcc cgtcttgtca aaacaccgcg
    10021 ttacgacaaa gatattccta ctctagctcg tgccgcaaaa aaaatgtttg aataatcaag
    10081 aggttgtgaa aaagctgccc tgcatcaagt aataagaaca accaggctaa aacagcttct
    10141 tatgtttttg atttggttgg acttattacc gcagatgcaa gcttttgaac accgtttacc
    10201 aagaggttat gaaaaagctg ccctgcatca agtaataaga gcagccaagc taaaacagct
    10261 tcttatgttt ttgacttggt tagacttatt accgcagatg caagcttttg aacaccgttt
    10321 accaagaggt tatgaaaaag gcgtttagtt tcgagtatga agaaaaattt tgtgaaaatg
    10381 gttcctcacg tttttgcata aatttgactt aataccgaga aactgatccc ttctgtcata
    10441 acctcttttt atttatgtct gtttcttttt gaaaacaact attttttcaa cgtttactct
    10501 cttgccaatc caacatcatc taacggatac aactatttca atgattacca ttacactaaa
    10561 aaatcggaaa cacacctctc cgaacacaaa tacataagac tatagaatgt ttcatttaag
    10621 aaaaaaagcg tttcattatc aaaaaggtaa gatctccttt aaaatgaaat tatctgtagt
    10681 actaataatg cagacaattt tatgaatctg aaaggagcat ctccaatgaa agcagtcact
    10741 tggcaaggaa aacagaaaat ggaaatcaga aacgtagatg atccaaaaat attagaacca
    10801 acagatgcaa tcatccgtat cacagcaaca gccatttgcg gctccgatct gcatctttac
    10861 catcatggtg aagcagtttt ggaaaaaggg tatgttgtag gacacgaacc aatgggaatc
    10921 gttgaagaag tgggatctca agttcaaaca ttgaaaaaag ggcaacgtgt ggttatccct
    10981 tttaatatta gttgtggtca ttgtcatttc tgcctgaatc acatggaaag ccaatgtgat
    11041 gaatcaaatc cagatcagtt atatggcggg cttttcggat ttggcaagtt gaacggtaat
    11101 cattggggtg gtcaagcgga atatctaaga gttccttatg cagactctac ttcatttgtt
    11161 gtaccagata atgatcttcc tgatgaaaaa gttttatttc tatcagatat tttgcccaca
    11221 gcttattgga gcgttgaaaa cgcaggagta aaaaaaggcg atacagttgt gattctaggt
    11281 tctggtccag tcggtctatt cgctcaaaaa ttcgctgtta tggctggagc agagcgcgta
    11341 atcgcagtag atccagtaca gcaccgtcta gataaagcgg ctaattacaa tggggttgaa
    11401 acttatttct tagaagacac ggcacaagct ggaacagaat tgtatgaact ggctaaaggt
    11461 ggcgcagatg tcattatcga ttgtgtagga atggacggat tagagcctgt caaagaaaaa
    11521 gcgaaaaatt tagtaagctt acaaagcggc acaatctctc cattacagat ggcatcgcaa
    11581 gctgtcaaaa aatttggaac agtccaagta actggtgttt atatgactcc agcttcttct
    11641 tacccgctgc aagaattttt tatgcgggat atcgctgtca agcatggaca ggcaccaatt
    11701 atccatctaa tgccaaaaat ttacgcaatg attgcagaag gactattcga tcctacacaa
    11761 atcattactc attcgctgcc actatcagaa gcagcacaag catatgagat ctttgacaaa
    11821 aaagaagata aaaatataaa agttgttttg aaaccttgaa ttcagtaaaa aatacaggtg
    11881 attttcttta tgcccaaaat caaacgaaat acccatgaga tcgaaatagg aaaagaagaa
    11941 gatatcgaaa tacgaggacg tcgcttccgc ttctatttcc aaaatcgtta cacattgatc
    12001 tcactggctg tcgatttatt aaccgggatt ttctatattt ttggaagcct tgcttcctta
    12061 actaaaatcc ccgatcgata tggcacctat ttttatttgg ctggtgctat tttcttgacg
    12121 attcgtccta ttctcagaat cgtaaggaac atttttattt acgacgaaaa aaaagcaatg
    12181 aagagaacaa gcaaaaaaaa agaggcatga gaatttttca aatctcatgc ctcttttttc
    12241 accctatcgt atcactttga tacagacgag cggtcgtccc actttcttcc ttgaccattg
    12301 tcaaaaatgt ccactcgtat cgttcataaa acgtggtatg atctgtaatt aagtaaagtt
    12361 tttcgtaccc aagttgatgc atttcttttc tgatttccgc taatatccgc ttggctatcc
    12421 cctgcttacg cttttccggt tcaacaaaca gggcacaaag atttggtgtc agatcagggc
    12481 ggttatggaa gtcgttttca atgacgcccg ctcctccgac gatggtttct ttttcatcta
    12541 agaccacata ccattgagga atactttcct tattagcgat tccagtacga atactttcct
    12601 catacacaac agcaggaact ccccattttg atgaaaacca attcgcgctt tgttttacta
    12661 attctgggtg ttctcttaat ttgatccagt tatatgtaac caatgtttaa acctccgact
    12721 aaaataaaac gtttacaaaa attatacaag tctttatgaa aattagcaat gacaacaata
    12781 gtgatgtaaa aaaaaaaacg gaaaacaatc acttgttctc cgttttttga ttaatcaagc
    12841 atctaaagct tcttttcttc tttcttggat cgtagctacc ttgacgaacg gtagatagat
    12901 caaaatagaa agaaccaaat tgacagcggc tagtacacca cctgcaatac tttttgttgc
    12961 caatacaccg ccaataattg gtggagttac ccatggaggc ataacggtag ctgctggcac
    13021 taaacctgta gaagtcgcaa agtaagcaac actaacta
//
LOCUS       DACDIH010000070        13019 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.070, whole genome shotgun sequence.
ACCESSION   DACDIH010000070 DACDIH010000000
VERSION     DACDIH010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.070"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            279..1016
                     /locus_tag="IV281_002178"
     CDS             279..1016
                     /locus_tag="IV281_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP8781153.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            1141..1620
                     /locus_tag="IV281_002179"
     CDS             1141..1620
                     /locus_tag="IV281_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP8781154.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            1617..2384
                     /locus_tag="IV281_002180"
     CDS             1617..2384
                     /locus_tag="IV281_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781155.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            2614..3183
                     /gene="lepB"
                     /locus_tag="IV281_002181"
     CDS             2614..3183
                     /gene="lepB"
                     /locus_tag="IV281_002181"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8781156.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(3287..3889)
                     /locus_tag="IV281_002182"
     CDS             complement(3287..3889)
                     /locus_tag="IV281_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8781157.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            3995..4464
                     /locus_tag="IV281_002183"
                     /pseudo
     CDS             3995..4464
                     /locus_tag="IV281_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            4570..5154
                     /locus_tag="IV281_002184"
     CDS             4570..5154
                     /locus_tag="IV281_002184"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP8781158.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            5157..6230
                     /gene="prfA"
                     /locus_tag="IV281_002185"
     CDS             5157..6230
                     /gene="prfA"
                     /locus_tag="IV281_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP8781159.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            6223..7062
                     /gene="prmC"
                     /locus_tag="IV281_002186"
     CDS             6223..7062
                     /gene="prmC"
                     /locus_tag="IV281_002186"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP8781160.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            7055..7234
                     /locus_tag="IV281_002187"
     CDS             7055..7234
                     /locus_tag="IV281_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781161.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            7256..8281
                     /locus_tag="IV281_002188"
     CDS             7256..8281
                     /locus_tag="IV281_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP8781162.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            8456..9700
                     /locus_tag="IV281_002189"
     CDS             8456..9700
                     /locus_tag="IV281_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP8781163.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            9853..10800
                     /locus_tag="IV281_002190"
     CDS             9853..10800
                     /locus_tag="IV281_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8781164.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            10934..11563
                     /gene="upp"
                     /locus_tag="IV281_002191"
     CDS             10934..11563
                     /gene="upp"
                     /locus_tag="IV281_002191"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP8781165.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(11702..11944)
                     /locus_tag="IV281_002192"
     CDS             complement(11702..11944)
                     /locus_tag="IV281_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781166.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            12065..12137
                     /locus_tag="IV281_002193"
     tRNA            12065..12137
                     /locus_tag="IV281_002193"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12098..12100,aa:Thr,seq:cgt)
     gene            complement(12256..12894)
                     /locus_tag="IV281_002194"
     CDS             complement(12256..12894)
                     /locus_tag="IV281_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8781167.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         4515 a         2208 c         2718 g         3578 t
ORIGIN      
        1 gtgataagac cgtcatcaat atataataaa aaacgatgat ttggatcgat gagaaatgat
       61 tggcagcaaa cttctgtcct cgccgctgca gccttcgaaa gaataaatca acatgcacag
      121 tactatcctc ctgacaatag tcgtttctac cagtatacaa ggggaattag gaatactcaa
      181 agaaattatc aaacagataa caatacagct ttcgattaaa atagaaatgg cttataataa
      241 ggaaaatcct agaaaaaaga aggaggagga ataacaaatt gattaatgct caacaaatcg
      301 tagaaaaaat gaaaaatcaa aaaataaatt atgatacagt tctgaaaaaa atgatcatgc
      361 aatgggagaa agaagaagta aagcctaaga tattaataca tacatgctgt gctccttgca
      421 gtacgcacac attggaattc atgtctcaat atgcagagat cacgttattt ttttcaaatt
      481 cgaatattca tcctaaaagt gagtatttgc gaagattaca tgaacaaaaa cgatttgtag
      541 accaatttaa tgagaaaact gaccatcatg ttgcgctaat cgttgatgaa taccgtccaa
      601 atgaattcac caaaatggtt ttaacaaacc atcttgaaaa cgaaaaagaa ggtggtgctc
      661 gctgcagtgc atgcttcaat atgcgattag atcttgttgc gcaaaaagcg caagaactag
      721 gatacgatta ctttggaagt gccctgacca tctcgccaaa gaaaaatgca gctttgatca
      781 atcagatcgg aatggatatc caaaagattt acgggacaca atacttgcca agtgatttca
      841 agaaaaacaa aggctatgaa cgctctttgc aaatgtgcaa agagtatgat atttatcgcc
      901 aatgttattg tggttgcgtg ttcgctgcca agcagcaagg atgcgatctg aaagaaatca
      961 atcgggaagc aaaagcttac ttaaaaacat caaccgtagt ttttgaggaa aattaaagaa
     1021 atctttcgga aaaacgtaag aaaaaaataa agaatttatg gtagaaataa ggtagactgt
     1081 ataatttgtc cgtagtaaag taaggaaaca agaataaatt tcaatgaagg aagagataag
     1141 atgcgaaaag tagcacctat tttgaagcca atcgctgtat taacaatctc gacgattgtc
     1201 gcgactacta caggtattgt gaatagttcc agctaccgtg caccaagttc aagttctagt
     1261 actgtagaag aaacaacacc aactacttca gtctcgacat ctgaaccaac aacaacggaa
     1321 agtttgaatg aaaccactac gacaagtaca tgggacgata caacaactag ttcgtcgtat
     1381 gatgagacat atacaactga cagctcaagc gaaacaagta atacggtacc agatggcact
     1441 ccatcggatt caaccaatcc aagtacaagt ccggatacaa gtgctagctc cgaaacgata
     1501 ccaagcagta cgactgtaga tgataacacg caaaatgatg atgcagcacg tacagcacag
     1561 caaaaccaag attcaggaaa tacccaaacg tataaccagc aagcaggtga ttctcaatga
     1621 catttttact agaccttatc aacggggtgc ataaattcct tggctattta gacatcagtc
     1681 ctaaatatct taaccgtgga tatacgattt taagtgtgat tcccacgctt tatttattga
     1741 gaattgttta tggactttgg caaaaccaaa actatcttca atttttctta tatggacttg
     1801 catttctagt gcttgtttat tttactgttt taaatgtatt ttattacttt ttagataaaa
     1861 acacgaaagc cgatgtcact cagctttttg tgaagtattt acctgatgaa gcgttcaaca
     1921 tccagacaga agaatcgata gataatggaa attcgatcga taaagtgaat acacaagagg
     1981 tcatggtttc ttatgacgaa gattatcaat tgaaattagc cgagaatatg cggtatttga
     2041 ttggaaatgg tgagatcaaa acaaatgaac tcggtcgcat ggatggattt ctggtcgata
     2101 gaaatacatt gtatccttat tactatgtca agaaaacagg agagaaagag tatgttctaa
     2161 atattggacg aagctactcg gacatggaaa aagtagggac cattcgactt gactcaacag
     2221 aagacacatt ggaacctgtc ggattgttca tcgtcggcgg tgattttgtc aaagacgggt
     2281 tacgctatca tgagccttat cgtctgaaat tgattgcaaa aaaggcacag tcgcaggaag
     2341 aaccagtggt ttatagtcgt agccaaagaa gaagacattc ataatgtgat gaaacgccgt
     2401 gtatcagtgg ttgcgataaa aacgtttatt cggaataagg gggatgtgac acgtcttttg
     2461 tcacgttcct tttttatata acgagaaaga taagacgaac acatcactta ttctttcctt
     2521 tcatttataa acattgtaga ataaagaaag tacaacgaag tattgtgaaa aaacgtaata
     2581 aataacgagg aaaatacaag taggaggtaa gctgtgaaag aatttttgaa aaattggggg
     2641 attttgatcc ttgtattagc ggctatttta ttagctagag tgtacgtgtt tacaccagtt
     2701 actgtaaacg ggcattcgat ggaccctaca ttaagtgatg gacaacgatt gatttcatct
     2761 aagatctcaa actatgaacg aatggatatt attactacaa aggaaccggg ggatgaagaa
     2821 cggatgattg ttaaacggat catcggtatg cctggagata cagtcaagat ggaaaatgac
     2881 cagttgacca ttaatggcaa aaagtatgat gaaccttatc tggatgagtt caaaaaagaa
     2941 ttttcagaag ataagttaca aggagaatat gcttatagct caggattcca agcacaagca
     3001 gaaagttcat ccacctttac gagtgacttt gaatatactg tgccaaaagg aaagtatctt
     3061 gttctaggcg ataaccgctt gatttcaaaa gacagccgaa tgtttgggtt agtcgacaaa
     3121 gatatgatcc aaggaaaagt tgttttccgt tattggcctc tttcagaaat taaaatcatg
     3181 tgatgaaaaa ataaaaagaa gctgtgacat gttttttgtc acagcttctt tttccgaaaa
     3241 ttccttctta tttcttggag ttgctcactt tttttgcaat ctcattttaa gctttttggc
     3301 tgatggataa gaaaagcgaa gcgtttttgt tcataaatag cttttcccca cgatggaagg
     3361 tatgactagt agcagagaca aagcccgttt tttccaatcg attattcaat tcttcttggc
     3421 taaagccatt atggactttc ggatgactaa tttgttcatt cttgtcaaag tctacgatga
     3481 tcaattgacc gccaggagct aaaatttcat aaagttttgt aagaatattc tccgtatcag
     3541 gaatgtgtaa aagtacaagt gatagaagga tgagattagc ttggacagca ggaaattcga
     3601 cagaagcatc agcgtggatc gttcggacat ttttcagatc tgctgcttgg atcttttctt
     3661 ctgccatttt cagcatagtt tctgaagcat cggcaatgat cagttctttg aatctttccg
     3721 caagcggtaa actaacaagc ccagttccgc ctccgtaatc cagcaggact ttttggtggc
     3781 tgtcgcgcgg tagataagta gttagttctt cgcgaataat cttggctaat gcttgacggt
     3841 caggggaatc ataatggtta gcgatggaat caaaaatatt ttttgtcatc aaatagctcc
     3901 ttacgtaaaa ctttgaaatt ttaacttatt atagcacgtg aaaagaaaga aagcatgcta
     3961 tactcaattc aaaaagggag aatctagaaa gaatatgccg atatcgtttg taaaagatag
     4021 agaggaaaaa ggaaagtgtg tccgagaaat attactagac ttgcctgagt ggtttgggct
     4081 tcctgaatca acggaaaaat atatcgagga atcaagtaaa ctacctttat ggtgtgaaaa
     4141 aagaaaagaa gaatatttag gctttatcac tctttcacag acaagtgaag atactgccga
     4201 aatctattct attgtatggg aataaaaaaa gactatcatc accaaggatt aggaacaaaa
     4261 ctttttaaag aagcagaagg ttacgcagca aaacattaca aatatttgca agtgaagact
     4321 gtagacgagg ggcactactc tatatatgat caaactattt gtttctacga atcattagga
     4381 ttttcaaggt tggaagtttt tccaaattta tgggatgaat ggaacccatg tttagttctt
     4441 gtcaaaaaat tggaacaaaa gtgagcaata gatttaatac taatctgtgc ttgatgaaaa
     4501 ttctcactgt aaaaaatgct gaaaatcaag tataatacca taggaacaaa ctggaaggtg
     4561 aaaaagagca tggcacaact attttttaag tatggcgcaa tgaatagcgg caagacgatc
     4621 gagatcctaa aagtcgcaca taattatgaa gaacaaaata agccagttgt cttaatgaca
     4681 agcggtattg atacaagaga tggtttaggc gttgtttcaa gccgaatcgg actgaaacgt
     4741 aatgcgatgc cgatatttga agaaacagac gtatttgacg taattcagaa aatggaaagc
     4801 aaaccctatt gtgtgctgat cgatgaagcg caattcttaa aaaagaaaca tgtcattgct
     4861 tttacgagaa ttgtggacga attggatatt ccggttatgg catttggttt gaaaaatgat
     4921 tttcgaaacg agttatttga aggatcaaaa tatttattgc tttatgcaga caaaatcgaa
     4981 gaaatgaaaa caatctgttg gttctgccac aaaaaagcaa tcatgaattt acattatatt
     5041 gatggaaacc ctgtttatga aggcgatcaa gtgcaaattg gcggaaatga agcttattac
     5101 cccgtttgtc gaaaacatta ctttcaccca tcaatgatta cagaaggaga ataagcatgt
     5161 acgatcaatt acaagcaatt gaagaccgtt atgaagagct tggagaactt ttaagtgacc
     5221 cagaagtgat ttctgataca aagcgtttta tgcagctttc aaaagaagaa gcaaacacgc
     5281 gcgaaacagt cgaagtttat cgtcgttata aagaagtagt acaaggaatc accgatacag
     5341 aagaactgct tggtgaaaaa ctggatgatg aaatggctga actagcaaaa gaagaattat
     5401 ctgaattaaa aaaagaaaaa gaagtcttag aagaaaaaat caagatcttg cttttaccaa
     5461 aagatccaaa tgacgataaa aatatcatca tggaaatccg tggagcagca ggcggagacg
     5521 aagcagcatt attcgctggc gatcttttca atatgtacca aaagtatgct gaagcacaag
     5581 gctggaaaac agaagtgatg gaagctaacg ttaccggaat cggtggatat aaagaagtga
     5641 tcatgatgat caccggagac aacgtatatt cgaaattaaa atatgaaagt ggtgcgcacc
     5701 gcgtccagcg tgttccttca actgaatcgc aagggcgtat ccatacttcc accgcaacag
     5761 ttgtcgtgat gccagaagct gaagaagtag agatcgatat tgaagacaaa gatatccgta
     5821 cagatatcta tcatgcttca ggtgctggcg gacagcacgt caataaaaca gcatctgctg
     5881 tacgtttgac tcacttacca acagggatcg ttgttgctat gcaagatgaa cgttctcaaa
     5941 tcaagaaccg tgaaaaagca atgaaaatct tgcgtgctcg tgtgtacgat aagatccagc
     6001 aagaagcaca aagcgagtat gatgccaatc gaaaatcagc agtcggaact ggggatcgtt
     6061 cagaacggat ccggacgtat aatttcccgc aaaatcgtgt gactgatcat cgtatcggat
     6121 taactatcca aaaattagat cagattttag cagggaaatt agatgagatc gttgatgcat
     6181 tgatcatgta tgatcaaaca tcaaaactgg aagctatgca aaatgggtaa aatgacctat
     6241 cgagaagtcc tgtcacgggc ttcttctttt ttagaggatc atgggaaaga agcatatagt
     6301 atccaatttt tatttttgga acgaaaacaa tggaaaaaac ttgattggct ccttcatatg
     6361 aacgaagaga tttcagaaga agaacagcgg ttgatcgaaa ccgatctgcg acttctgctt
     6421 gcagatcatc cacctcagta tttgctagga tatgcagatt tttatgatca tcggttaaag
     6481 gtaactgaag caacactgat ccctcgacca gaaacagagg agttagtcga atggtgttta
     6541 gacgagacgc ctggtgtacc gttagaagtg atagatattg ggacagggac tggagcaatt
     6601 gctattagtt tgaaagcagc aagaaaaaat tggcatatat ctgctgtgga tctttcagaa
     6661 gaagcattag aagtagcgaa agaaaatgct cagaaagaag gaacgaaaat cagtttttat
     6721 catggagata cattaggacc agtaatggat cagcaatttg atgtgattat ttctaatccg
     6781 ccgtacatca gtcggaatga atgggaactg atggatgaaa gtgtccggtc attcgaacct
     6841 aaaatggccc tttttgctga gaacgatgga ttagctattt atgaaaaaat agcagttgaa
     6901 gcaccaagcg tactaaaatc aaatggaaag atttttttag aaatcggatt tcgccaaggt
     6961 gaagcagtaa aaaaaatatt tcagcaagca tttccagata aaaaagtggc gataaaaaag
     7021 gatctttttg gcaatgaacg aatgatccaa gtttatggtt gaagttgtgg atgaaaaatt
     7081 ttttatttgt ttttttatta taaaaaaaca cttatttgac aaggatgtaa acgacttttt
     7141 ggtttatcaa caagtttatc cactattgac aaatagatta tccacaaaca gtggaaaact
     7201 tgaggataaa ctcagacaaa ataagaatgt atgaaaaagg ggttgtggat aaactgtgga
     7261 aacgaaacga tatgatgaaa cagaattgca agaggcagcc gaagcactga aagaaggaga
     7321 acttgttgct tttcctaccg aaacagtata cggattgggc gccaacgctc ttttaccaaa
     7381 cgctgtcaag aaagtgtttt ctgtaaaagg gagaccgcaa gataatccat taatcgtcca
     7441 tgtcgcctct tttgaacaag taaaagagta tgtggacaac tttcatccgg cagcagagaa
     7501 gattgtgaag aacttctggc ctggaccatt aactttgata tttaagatca aaaaagacac
     7561 tttgccctca gttgtgacgg gaggattatc cacagctgca tttcgtatac cggataacaa
     7621 aaaaacacta gaagtaatcg aactttccgg tgttccgctt gtcggtccaa gtgcaaatac
     7681 gtcgggaaaa ccaagtccaa cgactgcaga tcatgtatat catgatctac aaggaaaaat
     7741 taccgggatt attgatgatg gagctacgcg aattggtgta gaatccactg ttcttgattt
     7801 aagtgatcca acagccatgc ctatgatttt acgaccaggt gcagtaacga aagaacaaat
     7861 cgaagcagtc atcgaatctc ctgttgctat tgatcagcat cttgtaaaag aaaatgaaac
     7921 accaaaagcc cctgggatga aatacaaaca ctactcaccc gatacacgtg ttctgatggt
     7981 cagagatggc gattggtcaa ctgcagttca atgggcaaaa aataagaaga tccgtgcagg
     8041 tgtcatagca agccccgaaa ttgcggatca agtgcggaca gatactgcag ctgtttatat
     8101 gtataacgac aactcagtag aagcggcggc taaaggattg tttgcaggtc taagaggatt
     8161 agatgaacca acacttggat tagatttgat ttttgtacaa gtgtatccag aaactggatt
     8221 ggggaatgct tacatgaatc gcttgaaaaa agcagcagga caaaattatt ttgaaaaatg
     8281 atcaattggt tattgtcgca ttcttttagt atgatacaat gctatcaagt aaatcttcta
     8341 tcagtgaacg aaaaactaag cgtaactaat cgtaatagaa gagatacgaa tatttggcaa
     8401 tttgtttttg atgggaaaaa taaaattgta ggaaaataaa aaggagctgt tgtttgtggt
     8461 agattacaaa acgtttgacc ctgatctttg ggctgcgatc gccaaagaag aagaaagaca
     8521 agaacataat ttggagctga ttgcctctga aaactttgtc tccgaagcag tgatggctgc
     8581 acaaggaagt atcttgacaa ataaatacgc agaaggttat ccagggcatc gctattatgg
     8641 tgggtgtgaa tttgtcgata tcgttgaaaa tttagcgatt gaccgtgcaa aagaattatt
     8701 cggagcgaaa tttgcgaacg tccagcctca ctccggctct caagccaaca cggctgctta
     8761 tctcgctttg gtagaaccag gggacacgat cttaggaatg gatctttccg caggcggaca
     8821 tctaacacat ggatcaccag tcaacttcag tggcaaaacc tatcactttg ttgcttacgg
     8881 tgttgatccg acaacagaag ttattgatta taatgtggta cgtattttag ctagaaaaca
     8941 tcaaccaaaa ttgatcgtag caggtgccag tgcttacgga cgaacgatcg actttgcgaa
     9001 attcagagaa atcgcagatg aagtaggcgc aaaactgatg gtagatatgg cacatatcgc
     9061 aggattagta gcagcaggac ttcatcctaa tccagttcct tatgcagata tcacaacgac
     9121 taccactcat aaaacattgc gtggccctcg aggcggcatg attttgacaa acgatgaagc
     9181 attagcaaaa aaaataaata gtgcggtatt tcctggtatt caaggcggcc ctctagagca
     9241 tgtgattgcg ggtaaagcag tagcattcaa agaagcatta gatcctgcat ttaaagaata
     9301 tagtgaacaa atcatcgcca atgcaaaagc aatggtcaaa gttttcaatc aagcaattgg
     9361 aaccagagtc atttcagggg cgacagataa tcatctgatg ttgatcgatg ttcgcgagtt
     9421 aggtataaat ggaaaagaag cagagagcat tttggacagt gtgaatatca ctgtaaataa
     9481 aaactcgatt ccttttgaaa cactaagtcc attcaaaaca agcggtattc gaatcggcac
     9541 gccagcaatc actactcgcg ggttcaaaga agaagatgct gtaaaagtgg ctgaactagt
     9601 tgtcaaagca ttacaagcaa aagatgataa cgctcaacta gacgaagtca aaacaggtgt
     9661 acgcgaatta actgaaaaat ttccgttgca taaaaaataa aaatttaaat tccattagta
     9721 aacagaaaag ttcaatgttg ggcttttctg tttatttttt gggaaaacgg attctttgac
     9781 aatatcggtg atgctctcta gtatatgagg tgtaatttta gtcttcacac taaatctcag
     9841 ggaggaaaag tcatgactga tcgtatctcg ttagaaaagg aagcagtagc attcagtgat
     9901 gcaaatgcgc ctcatccgcg tatctatgaa ctagcaccgg aagaaggaag agttttacta
     9961 gaagaagtac aagaatcacc tgtgaacaaa tattccgtcg atattgaaga cttgatggtg
    10021 gatactggcg aatggggaaa actcaatatt cgtctcgtac gaccagaagg gaatatggat
    10081 aaattaccag ttatttttta tattcatgga gcaggctggg tttttggcag tgctcaaacc
    10141 catgataaat tgatcagaga acttgcggtt cgtacgaatt ccgtcgttgt ctttccagaa
    10201 tatagtcgat caccagaagc aaaatatcca acagcaattg aacaaaatta tgctgtgttg
    10261 caacagctga ataaattagc caagtcaaaa aatctggatc tcgagaatct aatcgttgca
    10321 ggagattcag taggtggtaa catggctaca gtcatgacga tcttaacaaa agaacgaaag
    10381 ggcctcccaa tatccaagca attgctgtat tatccagtca caaacgctga ttttgataca
    10441 gattcctatc atgaattcgg agaaaactat tttttgacaa gagaaggaat gatgtggttt
    10501 tgggaccaat atacgacgaa tgaagaagag cgtgctgaaa tcacagcttc gcctttgaga
    10561 gcaaatataa atgaactaaa tgatcttcct tcagcaatga ttttaacggg agaagcggat
    10621 gtattaagag atgaaggtga ggcttatgcg cgcaaacttc gtgatgcagg ggttcctgtt
    10681 acacaagtac gcttccaagg aatcatccat gattttgtca tggtaaatgc tatggatcag
    10741 acaaaggcaa cgcgtgcagc aatggcatta tctacttctt ggatcaagaa aaaagaataa
    10801 gctttgcttg tccgcaggaa aacttctttt caaaaacgga ttttcttagg aaaaatggcg
    10861 agtttgacta gtcaatcact tgattttgtt ttacaattaa aaagaaacga cagaacaaag
    10921 gagagcttaa aaaatgggca aatttcaagt gatcgatcat ccattgattc aacacaaact
    10981 aactattatt cgggacaaaa actgcggaac aaaggttttt cgtgaagtag tcgatgaaat
    11041 cgctatgttg atggcatatg aagtatcacg ggatatgcca ttggaagaca tcgtgatcga
    11101 aacaccgatc actgaaacaa cacaaaaaac gttgtctggt aaaaaagtag cgattattcc
    11161 aatcttgcgc gcaggtattg gaatggttga cggtatcttg gaattgattc cagctgctaa
    11221 agtgggacat atcggtcttt atcgagatga agaaacattg gaaccacatg aatatttcgt
    11281 caaacttcca gaagacattg atgcacgtca gttgtttgta gtagatccaa tgttggcaac
    11341 aggcggatca gcaatcatgg caatcgatgc attgaaagaa cgtggcgcgt cgaacattaa
    11401 atttgtttgt ttggtagcag cacctgaggg agtaaaagct ttgcaagaag cgcatcctga
    11461 tattgacatc tatacagctt ctctagatga aaaattgaat gaacatggtt atatcgttcc
    11521 tggactaggg gatgcgggtg accgtttgtt tggtacaaaa taagtaaata cgaaaaaggg
    11581 tgtgacacaa cttttgtcac atcctttttt ggtcattttt aacgtttgat gcagaacagc
    11641 tttttcacag ctttcgtttg catataaaaa aaccatccca aaaatgcaga gatggtcaaa
    11701 ttcaaatctt ttttagttct ttgtagttaa cgattactac acccttactt tgcgtcatca
    11761 agttcgcatt ttctgcagtt tcatcaatct caacaacagc ggaatttttc aattgctttg
    11821 aaatcgttcc agtttgcgac ttgccgtgaa ttgtgaacat tacgtgagta ttgatcttga
    11881 atttttcacc aatttccgat ggtacgatag ttgtatcaaa ttttccaaaa gttgccattt
    11941 tcataaaacc tgcctttcac atcaattata ccacgtttta tgaaaaatat catgaaatta
    12001 aactaatttt ccatcggact ctttacaatc agccaaatag agtatataat ttcttttgga
    12061 taaggccagc ttagctcagt tggtagagca acgcactcgt aacgcgtagg tcgtaggttc
    12121 gatccctata gctggcatta aaagagaaga cagatatatc aaggattttt aatccattga
    12181 tacatctgtc tttttgttta aaggaaataa agtatgcttt cttagcaata gagcatgtat
    12241 tatttctata aaaatttagg agaataaaac gactagcaat atagctgtga aatcaaatat
    12301 cacatgaact gcccaacttg taatgatcga gttgctttta atggcagctt ggttgaataa
    12361 caatcttgcc accccttgta tcaaaaggat gtgtgctaca gtgtgtaaaa tatttccatt
    12421 atcataagta ctgtaatgga taaatccaaa gatgattgct gacaagaaac tagcgacagg
    12481 aatgctgaac ttactagaaa aaatagcgag aaaatacact gagaacaatt cttctccaag
    12541 tatcatgata ggcaaaataa taaataataa cgggctgaaa gcctcattta ttggattgcc
    12601 tgttaaaccc catttcaaaa tttgttgcaa aaataccgag actgcggcac tgattactac
    12661 attgataaaa aaatatttgg ctatattttt gacaggagcg acaggtttcg agaacatttt
    12721 tttcgtatct tttttaccaa aaacaagtgt aaatgctata aatccagtga tagttaaaga
    12781 aaaaatgcta ataatggatg gcgcaaatag caaccctaac aaaccgagta caacgacaat
    12841 agtgcccaat atcccttttt taagttctat tttttttcct aaaatgttat tcatgataat
    12901 aactcctact attcagtttt tgaagataga gtcagtatac atgaagagtt aaaataggca
    12961 atgttttttt atggatattt gtattgctaa aaatttatct tatatattca ttttgatta
//
LOCUS       DACDIH010000071        12877 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDIH010000071 DACDIH010000000
VERSION     DACDIH010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.071"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..639
                     /locus_tag="IV281_002195"
     CDS             <1..639
                     /locus_tag="IV281_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8781168.1"
                     /translation="GKSTIAKLISGFYNVDKGQIKIGGKPLNHYSKDTIINTIAFVFQ
                     DTKLFNQSIYDNVSIANPQAPASKVFDALRLAGCESILEKFPEREHTLIGSKGVYLSG
                     GEKQRIAIARAILKDAKIVIMDEASASIDPDNEYELQKAFQHLMKEKTVIMIAHRLSS
                     IKSVDEILVLSEGKIVERGNHNELMAKHQVYKQLVTNYENANDWRLDNEKFL"
     gene            593..2365
                     /locus_tag="IV281_002196"
     CDS             593..2365
                     /locus_tag="IV281_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8781169.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINM
                     VPAFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     MQIADDLSNLPLSYFSKHNLSDLSRTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            2888..3157
                     /gene="rpsN"
                     /locus_tag="IV281_002197"
     CDS             2888..3157
                     /gene="rpsN"
                     /locus_tag="IV281_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8781170.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            3172..3351
                     /gene="rpmF"
                     /locus_tag="IV281_002198"
     CDS             3172..3351
                     /gene="rpmF"
                     /locus_tag="IV281_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8781171.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            3383..3760
                     /locus_tag="IV281_002199"
     CDS             3383..3760
                     /locus_tag="IV281_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781172.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            3781..3930
                     /gene="rpmG"
                     /locus_tag="IV281_002200"
     CDS             3781..3930
                     /gene="rpmG"
                     /locus_tag="IV281_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8781173.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            3954..4937
                     /locus_tag="IV281_002201"
     CDS             3954..4937
                     /locus_tag="IV281_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8781174.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            4967..5089
                     /locus_tag="IV281_002202"
     CDS             4967..5089
                     /locus_tag="IV281_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8781175.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            5107..6630
                     /locus_tag="IV281_002203"
     CDS             5107..6630
                     /locus_tag="IV281_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8781176.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            6654..6893
                     /locus_tag="IV281_002204"
     CDS             6654..6893
                     /locus_tag="IV281_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781177.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(7086..7721)
                     /locus_tag="IV281_002205"
     CDS             complement(7086..7721)
                     /locus_tag="IV281_002205"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8781178.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(7742..7960)
                     /locus_tag="IV281_002206"
     CDS             complement(7742..7960)
                     /locus_tag="IV281_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8781179.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            8269..9096
                     /locus_tag="IV281_002207"
     CDS             8269..9096
                     /locus_tag="IV281_002207"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HAP8781180.1"
                     /translation="MPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPII
                     QAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDI
                     RGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILN
                     TNLFNIDETSYSMGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKN
                     FPGNITRAKGFLWFAQHSDFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            9285..9692
                     /locus_tag="IV281_002208"
     CDS             9285..9692
                     /locus_tag="IV281_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP8781181.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(9933..11273)
                     /locus_tag="IV281_002209"
     CDS             complement(9933..11273)
                     /locus_tag="IV281_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8781182.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(11549..11704)
                     /locus_tag="IV281_002210"
     CDS             complement(11549..11704)
                     /locus_tag="IV281_002210"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP8781183.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(11888..12091)
                     /locus_tag="IV281_002211"
     CDS             complement(11888..12091)
                     /locus_tag="IV281_002211"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8781184.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            complement(12001..12333)
                     /locus_tag="IV281_002212"
     CDS             complement(12001..12333)
                     /locus_tag="IV281_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8781185.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
BASE COUNT         4328 a         2001 c         2507 g         4041 t
ORIGIN      
        1 ggaaagagta cgatagctaa actaatttca ggtttctaca atgtggataa gggacagatt
       61 aaaattggtg gtaagccttt aaatcactat tcaaaagaca caattattaa cacaattgct
      121 tttgtctttc aagatacgaa actattcaat cagagtattt acgataacgt ttctatagca
      181 aatcctcaag cgccagcaag taaagtattt gacgcgctaa gattagccgg ttgcgaatcg
      241 attttagaaa aatttcccga aagagagcat accttaattg gtagtaaagg agtttatctg
      301 tcaggaggag aaaaacaacg tatagctatt gccagagcaa ttttaaaaga tgcaaagatt
      361 gttattatgg atgaagccag tgcatctatt gatccagata atgagtatga actacaaaag
      421 gcttttcaac atctgatgaa agaaaaaaca gttattatga tagctcatcg attatccagc
      481 attaaatctg tagatgaaat tcttgtcttg tcagaaggga aaatagtaga acgtggaaat
      541 cataatgaat taatggctaa acatcaagtg tataaacaat tggtcactaa ttatgagaat
      601 gcaaatgatt ggaggcttga caatgaaaag tttttataaa aacaaattcg ctttaactaa
      661 tcagggcgca gaagatttgg ttaaagcatc tatttctagc ttttttgtat actgcattaa
      721 tatggtacca gcattcatta taatgatgct gatagacgaa ttagttttag aaaatgtaaa
      781 gccgccttgg ctttatttta cagtatcgtt cgttactttg cttttcatgt attggttgtt
      841 agatagggag tatgaaaact tatataatag tacttataaa gaaagtgcgc atttaagaat
      901 gcaaattgca gacgatttgt caaatttacc attatcctat ttttcaaaac ataatttatc
      961 agatttatct cgaactatca tgtctgacgt tgaaggtatt gagcatgcga tgagtcatgc
     1021 aatacctaaa gccggcggta tggctctgtt tttccctttt atttcagtga tgcttttggt
     1081 tggtaatgtc aaaatgggat tagctgttat tttgccaacg ttatttagtt ttgtcttaat
     1141 cttgttatca aagaaatccc aaacgaaagc caatactaaa tattacgata ctttgagaga
     1201 aaactcggaa gaatttcaag aaactataga attgcagcaa gagattaata gctttaatct
     1261 atctaaaaaa gttcaagaca gacttttcaa aaaaatggaa gagagtgaaa ggattcattt
     1321 aaaggtagaa ttaagtactt tttcagtcat ggccttatcc tctattttct catatgttag
     1381 tttagcagta gtcattctag taggtgttca cttactgtta acgggtgaag tgactatact
     1441 ctacgtcgtt ggttacttac tagccgcaat aaaaataaag gattccttcg attctatgaa
     1501 agaagcagtg cttgaaatat tttatttagc tcctaaaata cagcgaatta gagctatgaa
     1561 ggaaaccagt atccaagagg gatcagatag tcctctgaaa tcttttgaca ttgagttgcg
     1621 agatgtctct ttctcatatg ataacaatac cccaatttta gatcatatct cttttacggc
     1681 taaacaagga gaagtgactg cgttagtagg tgctagtggt tccgggaaaa ctagtatctt
     1741 gaaattagtg tctagactgt atgattatga tgaaggctgt attttaattg atgggtatga
     1801 tattaaacgt gtatctccag cttctttgtt tagcaaaatc gctattgttt ttcaagaggt
     1861 aactttgttt aatacaagta ttttggaaaa tatacgtatt ggaaatagtc aagcgtcaga
     1921 tgaggaagtg aaaaaagcag ctcgtttagc taactgcgaa gactttattg aaaaactacc
     1981 tgacggttat catacattgg tcggagaaaa cggaagttca ctttctggag gagagcgaca
     2041 acgcctatcc atagcacggg cttttttgaa aaatgcgcca attttaattt tagatgaaat
     2101 tactgccagt ttagatgcgg aaaatgaaaa acgtattcag gaaagtttaa atcgtttaat
     2161 acaagataag acagtactaa ttatatcgca tcgcttgaag tctattgaaa aggtaaataa
     2221 aattgtagtg atggatcaag gaaaaatagt tgatcaaggt actcatagtg agttgtatcg
     2281 aaggtcagaa atatacaaaa atctcataaa gaaaactaaa ctatctgaga aattcgtcta
     2341 tgaaaaagaa gctcaatcac gatgatgaaa taggctttaa attaacctac aatttttggg
     2401 atatagttat aattcttttt caaaaagaat aattttatta aacagcatct aattttgaat
     2461 acacgaaaga atcagtatga ttgaattagt tcacttaatg tctattctta taatataagg
     2521 aataatcaaa gttttaaaaa attggcgata aaccagttaa acataaaaga ttgaataaaa
     2581 agttatagtt tctttcatat tgccgtttta agtagaggaa aagctactta aaacggtatt
     2641 tgtgtttgat tggactaata aaagtgaatt cttatttttc agagcttaaa ctgcatattc
     2701 ttttaaatga tttgattatc tctatgtacc gataaattcg ttttcaaata gttatctatt
     2761 catatgagtt ttatttcatg ttatttattg ttgacaagag caactcttgt tggtaaagta
     2821 tcagtgtcaa aacgtaacga ttacgatttg agatgtctaa atcaaaataa aaggagtata
     2881 aaggtatatg gcaaaaaagt cgaaaattgc taaagcaaag aaacaaatgg cgatgattga
     2941 aaagtacgca gataaacgtc aggaattaaa agccgcagga gatcgcaccg ctctcgctaa
     3001 attgccaaga gattcaaatc cgaatcgtct gaggcttcgt gatcagacgg atggtcgtcc
     3061 aagagggtat atgcgaaagt tcggtatgtc ccgtattaag tttagagaac ttgcccatca
     3121 agggttgatt cctggagtaa aaaaagcaag ttggtaaaag gaggaaatat catggcagta
     3181 ccagcaagaa aaacatctaa ggcgaagaaa cggttaagaa gaactcatca aactgtagta
     3241 aaaccagaaa tttcatttga cgaagcgaat ggtgattata gacgtagcca tcatgtatct
     3301 ttaaaagggt actataatgg aaagaaagta attaaaggag cttcaaaata gtttaatata
     3361 taacttttag gagggaatca aaatggaaat tatttaccca cctttagttg aagaaggatt
     3421 aaagtattat cttgaaacga cccagcaatc gctagataaa tgcacttttt atcgttcgat
     3481 ggtagagaga ggaattatta ctgaaacggg tttaccgacc caacaggcaa ttgaaaatgg
     3541 attagtcaaa gattactatg aggaccaagg cttatcattt gatgattttt taagaatcta
     3601 tccaattttt gaagagtatg acgaggagtt atttcaatgt attgatggtt attgggagat
     3661 acctgttgat atgaaagaaa atttagtttc acaattggaa tctggcgagt taacttttga
     3721 agatgcacaa caaattcaag catatcttga agatcgataa gtattgaagg aggaagattt
     3781 atgcgtcaaa atattatttt ggaatgtgtc gaaactggag aacgtctgta tctcaccagc
     3841 aaaaataaac ggaataaccc tgaacggtta gaattaaaaa agtattcgcc aaaattgcgc
     3901 agacgggcaa ttttcaaaga agtgaaatag ctaagtggta aaaaggtgac aaaatgggca
     3961 taccagttac aatcgtttct gggtttttag gggctggaaa aacaacgcta atcaatcaag
     4021 ccttgcaagt ctcaccctac cccagggaag aaattattat aattgaaaat gagtttggtg
     4081 aagttgggat tgatcacaaa ttattagttc atagcgagga acgggtgtta caactaaata
     4141 atggatgtat gtgttgcagt cttcgcagtg atttattagc aatattgatt tctttactgg
     4201 aaacaataga agaacatcaa caaccaattt cacaaatcat cattgaaact acaggtatag
     4261 cagatcccca accaatcatt caaacacttt taacagcgcc tcaactaaga ggaagagtat
     4321 atatcgatag tttattgact gttattgata gtgacagttt tgaacttatt gaaagagaac
     4381 cacaagccct taagcaactt gtcatagcag atcgcatttt tgtgtctaga aaacaaaact
     4441 tgaatttatc tgcactaaaa aaattacaga gagaaatcaa aagtattaac cctttatccg
     4501 atattcgaat gttcacacat agtgaaacga ttcaaaaaaa ggatttttat aatttggaaa
     4561 aattcaatca tcccttagtt tttgaaagtg attcatcaga aaatcactat aatggcaaac
     4621 actatcatca acatcatcaa gaacatgagt ttaaatcaat tctcttaact tcagatcaag
     4681 acataaaaaa agatttttta ttccaattaa ttgaccggct gattttaacc aaccaaggga
     4741 gcctgtatcg ttttaaagga ttgattgctt tgcaagatca agattttata gttgccatac
     4801 aaggggtaaa cgaacaggtt aattttcaat acaccactcg acaaaaagac caaaccacta
     4861 ttattctaat tggaaaaatg ttggatgaag aaaggatcaa acagagtttt gataaattac
     4921 tggcggaaag caaataatga taagtattga ttaggggtaa tttctaatgg agaaaacgga
     4981 tatatctagt gcatatcgac gacttaaaag cccgaatatc aaaactagaa aaagggcatt
     5041 aaaaattatc aaagaacata aacaaaataa aatgaagaaa ttagcataat taaggaagga
     5101 aaaagaatga agaaaatcag tgtaggactt attgggatag cagctctagg attattggga
     5161 gcttgctcca gcactaatga tgcaaaagta tcgaatgata aagatggaaa attagaaatt
     5221 gtaacaacat tctatccaat gtatgatttc accaaaaaca tcgtcggcga tgaagcgaac
     5281 gtggatttaa tggttccagc gggatcagaa cctcatgact atgagccctc agctaaggat
     5341 atggcaaaag ctcatgatgc tgatgttttt gtatatcata acgaaaacat ggaatcatgg
     5401 gtaccaaaag ctaaagaaag ttggaagaaa gctgggccta atgtagtaga aggaacaaaa
     5461 gacatgattt tattgccagg tagtgaagaa gaagatcatg atcacggaga agaagatcac
     5521 catcatgaac tagatcctca tacgtgggtt tcacccaaaa tggcaattaa ggaagtttca
     5581 aatatcaagg atcagttagt taaattgtat ccgaaaaaag caaaagtatt tgagacaaat
     5641 gctgaaaagt atttgactaa actgaaacgg ttggatgctg actataccac tagtcttaaa
     5701 gaagcgaaac aaaaaagttt tgtgacacaa catgcagcct ttggatattt agctttggat
     5761 tatggattga tacaagttcc tattgctgga ctaagtcctg aagaagagcc atcttctggt
     5821 cgattagctg aattaaaaga gtatgttaaa aaaaataaga tcaattatat ttactttgaa
     5881 aagaatgcta atgataaaat cgcaaaaact ttggccaatg aagctgggat aaaattggaa
     5941 gtattgaatc ctcttgaaag tcttacaaaa gaacaaatgg ataatggtga agattatgtt
     6001 tcagtaatgg aagacaatct aaaagcgtta gagaaaacaa ctatggttgc tggtgaagag
     6061 gtagttccag aaaaagaagc gaaggatgaa aagacagttg ctaatgggta ctttaaagat
     6121 gccgatgtga aggatcgcga attgagtgat tatacaggcg aatggcaatc tgtttatccg
     6181 ttgttaaaag atggaatttt ggatgaagtt ttcgattaca aagcaaaact caataaagac
     6241 atgacagctg ccgagtacaa agattattat actactggct ataagacaga tattgatact
     6301 atcaacatta aagacaacac cattgatttt gtagtcaacg gtgaacatca tcaatacaca
     6361 tacaaatatg ttggatacaa aattttgaat tatgaaaaag gaaaccgtgg tgttcgtttt
     6421 aattttgaaa ctgatgatgc aggtgctggt cgttttaaat atattcaatt tagcgatcat
     6481 ggaattgcac caagtaaggc agagcatttc catattttct ttggtggaga aagtcaagaa
     6541 aaactttata atgaaatgca taattggcct actttctatc cagcttcatt atctgagcat
     6601 gagattgctc aagaaatgat ggcccattaa aatccaatct ttggaggaaa tacatggtaa
     6661 gaaaagtatt attgtctatg gattccaaga atattgatgc ttatcggcgt tatcaatttg
     6721 caaagcaaat gagattaaat gggagtacat tagctgatat tgctgcaact ttacatgtta
     6781 gtattgacac agttcataga gtattaaaac gggatcctga ttgttgtttt atcaaggtca
     6841 atactggaag aagtagacgt ttagcaaaac ggcataatag cttggccaat taattgataa
     6901 taccagagta gatcattttt cccttatttg gatttgatct gctttttatt atttcttgaa
     6961 ttgtcaaatc aagcgcaaca ctttatcaaa gaagacttca taaggtgttc tccaatctaa
     7021 acactttcta ggtctgttat tcagtttttc aatcatctga tttatcgttg agttatctac
     7081 cgtagctatc tctttcgatt ttggcaagta ttctcgaatg agtctattcg tattttaatt
     7141 tgttcattgt tgccaagggc atgaggatct ggaaaataaa agggcatatt attcaatact
     7201 tctgtaactg cgaaatgatt ggagaattct tttcctctat ctggagtgat cgttttgacc
     7261 gcctcatttg gaatactgct taaaagttca atcattttct cgctcacata agcggctttc
     7321 tttctgggga ccttctttcc tgctaaaagg aagcatgatt ttctgtctgt caaagtcact
     7381 aaacaagtac cactagtttt gccagctaca atatcggctt tccaatgacc aaaatcactt
     7441 ctatcatctg cggattttgg atggccatgg atcgaatgag taaggcaaat cttcccacga
     7501 gactcgacat gattttttgt atgtcttgtt tttcctcgat ggtgcaatga acggatcagg
     7561 cctttattgc cgtgtgagag gggttctgta tcgaaaagat cattgcaaat tgcacggtag
     7621 atcgtgataa aactaattga aaagtccata tgttcatgct tcaaacgatt cgagattttc
     7681 tctggcgacc actgaaaatc gacaaataaa tgacgaacaa ttgaccaaac tcacggatca
     7741 tttagaatgt gataacgtcc gcattttttt cttcgataag cataattttt ttccgctttg
     7801 gaaggtgatt agtttttttc ttgtttcttc tcagttcatg taaaatagta aacgggctat
     7861 gctgaatgtc gttgccaatt atactaatag aaaatccttc ttcgtgaagc agaaagattc
     7921 tttctcgttt gagtatagta agatgacaat actggctcat aattattttc cttctaggag
     7981 tgttctcgac aaacttatac tagttggata tcgattatga ctcattttta tgtgttgcac
     8041 ttaaagtgta aattcaaggt atagaaagag gttccctatg aaaaaatacc tattacaatc
     8101 ttaactggct atcttggttc aggaaaaacg atacttctaa atcatattct gtacggtgat
     8161 cacggaaagg aaattgctgt cattgtaaat gacatagggg aagtaaatgt tgatggggta
     8221 ttagtcgaca agaaaggttt taaaagaact ggagaaaaac ttgtttcgat gccgaatgga
     8281 tgcatttgtt gtacattacg cgaagatttg attgttgaac tagatcaatt agtgcaattt
     8341 tctgacttga atcaaattgt tattgaagct tcaggaatca gcaaaccaat tccaattatt
     8401 caagcaatta tattagctaa aaacccttta gacattgatt tgacccaacg attagagatt
     8461 ggtgccatta ttatagtagt ggatgtaaaa cgtttttggg ataatttcga aacgggtgaa
     8521 aagattcagg aaagaaaagc tgaaggtaat gtagttgaag aagaacagga tattcgtggc
     8581 ttgttaattg atcaattcga gtgttgtaac atattacttt taaataatag tgacttagtg
     8641 tcaaccaaga cgatttacaa aattaacggt ctaattgcta agttacagtc caatgccaaa
     8701 ataattagga caattaaagg tgaagtagac ttatcgaaaa ttctaaatac aaatcttttt
     8761 aatatagatg agacttccta ttcaatgggc tggttcaaag agttagagct tggctatcaa
     8821 aatcatattc ctgaaacaga agaatatggt atctcaagtt ttatctatcg aaatcggtta
     8881 ctccttgacg cacaaaaatt tgcccatctt acggataaaa attttcctgg caatattaca
     8941 agagcaaaag ggtttctttg gtttgcccaa catagtgatt tttcgacgat ctttgaaaca
     9001 tcgggtagcc ataaaagtat tgaatcatta aattattggg tagctagttt acctaaaatt
     9061 gagtgtcaaa tgattataca aaaaaacccg aattgaagga agcctggcat ccgttgtttg
     9121 gtgaacgcat taataaacta gtttttattg gtatgaagat ggatcaacaa tgtattaagg
     9181 aacaacttga gggttgttta actgattctc ttaatgcaga taatttccga aaaatttcac
     9241 catttccatg gaaaaattct aatatgttta gccagatatc gttaatgaaa tttttaagaa
     9301 aagctggtgt caagataggc gatgtcataa ttattggcat tttgattatt ccctctttta
     9361 ttcccttatt tgtattctct atagaaaagg agaatgaaac aactgaagat gctatcaaat
     9421 atgccatcgt tcaaattgat ggcaaggaaa tggatcgctt cgatttggat aagatcaatc
     9481 ataaactcgt gaattatcat cctgcgaagg gacaatataa tattgttgag attaaagatg
     9541 gaagaattcg agttaaagaa gataatagtc ctgatcaaat cgcggttaga actggatgga
     9601 tctcaaaacc cggtcaaacg agtatttgtt taccccataa gttagttatt agtattgaga
     9661 aaaaagaaag caaagattac tacatttact aatctggaaa tagatgagga ggtttttaat
     9721 aagaaaaaga tattattatt aataatagca ggggctttat taagtgtgtt aagcgcttgt
     9781 ggacaagaaa aagaagcggt gacaacaggc acaagtatct ttaacgggac ctgcaatggt
     9841 ttagttaaca gtcggttaga ttacttattt tggctcttcg tcaatgagtg ttggtgaaga
     9901 aattcaatag aataatcgag atactcacac ggctagacag ctatcttgtc tagccgtgtt
     9961 gttgttctgg ctttagtcag ttgggtagtg ttcttagttt tcttgaaata ttttcctcga
    10021 tataaaaaga ttcgttctcg gtagttttga aagtttctaa agccataggc aattcgtttt
    10081 agtactttga tatggttgtt caaacattct atagggccgt tggaataggg aactcgcaac
    10141 gctcgtttga tttctgttcg atatttcttg aaggtcgtga acactggttg gtagtgctct
    10201 ggcaactcct tgtagtcttc ttttaggagc gcgacgaatt caggaacatc ttgtcttctg
    10261 atagcggaaa gaaaaacctg gtagacttcg tagcctcgct ttaaggagtc atcataatat
    10321 aatagccgat cgactatctc cgcttctgtc agatgatctc gaaatgacgg acgccagtag
    10381 cgatgctgac cattcaattc ccacgcattc ttttgaagta gtctccagta tttctttaag
    10441 tgcttcgacc gacgatcccc ttttttaaag gtcttcatta cttgaattcg gtggttggta
    10501 aaggcccgac cgatgtgttg gctaatatga aagcgatcgg cgacgattcg ggcattcgga
    10561 aaacactctt tcaccaatgt gcgatagggt gtatagaaat ctgaaaccac tagacgtact
    10621 tgttcacgga cttttctagg gtagcgcaag aaatagtcgc gtaaaggatt gagttgccga
    10681 ttttccacaa tatcaatcag ttgatgggtc ttgccgtcca tcatgacaaa gctcatggct
    10741 cccgagacgt ttttcaccga tttgaattca tcgaaacaca ccacttctgg taacggctgg
    10801 ttcaaagaaa cggttttagg ccgaaggctg cgaagtattc gaatgacagt ggtcggagaa
    10861 atgtgcttca tacgtgcaat caaagacata gaaataggct cacttaatag ctcaagaatg
    10921 gcttgtttga ctctgcgggc aatggagcag cgacgatcgc taactgaatc ttctgctaaa
    10981 aaagtctttc cacagtgttt acatttgaag cgctgtttgt taatccgcac aatggtcttg
    11041 tagttactga catcattcaa taagatggat acttttttcc agccccattt aatgatcgtt
    11101 tgcttatttt gatagtggca atggaagcat tcctctggtt gataggtcaa tgtgccatca
    11161 aaaacaaaac atgtttcgtc attaatgcgc tctttgcgaa aggcatcttc atgaaaggta
    11221 agatttaggt ctagaatatc tagtaactct ttggtaaact ctgaatagga catcttgaaa
    11281 cactcctttg aatggttttc gtcgacttta ttctaaagga tttcaaggtg tctgtttgcg
    11341 tttattagaa aaaagaaaaa gtattggcaa cagaaggcgt caccaacact agatattata
    11401 gatcccttat tttcttcgct tcaaattgtc aaggagtcat cataaatttt gtatggattc
    11461 ttttgtttgg gtagtagtct tctaaataat cttggattaa caccttcatc tctattttac
    11521 ttttatattt gttggagtcg attaattctc atttgattga tcgatgaaac gcttcaatta
    11581 ctgcattgtc aacaggagtc ccagtttggc tcattgaatg tgtgatgtgg tggtcggtca
    11641 acagctcttg ataaataaaa gaccgatgct ggctacctct atcggaatga attaccgtag
    11701 gcaattctcc ttaataaatt tgtaatggac ggacgacaat ctcttttttc atgtgtgaat
    11761 ccaccatata gtaacgtaga atacgagttg ctaagtcaat ataggtggac aaatagaggc
    11821 gaccgtcatt acatggaatg taagtaatat ccgtcaccca aacagcatca atttggttct
    11881 gttcaaatta tcgattcaac atttttttta ccatttcttt gattgttttt tctctaccat
    11941 gagaagattt gcgtcgatga aatcctcttg tgcataaatt catttcacgc ataagaacag
    12001 ctacaacacg tttattgatt tcgattcctt catcgtaaag tttttgagtg atccctggag
    12061 atccgtatcg atctttgtgt tcgtagaaca ctcgtcttcc tcttttagtc gtttattttc
    12121 ctgttcaaga atgtagaaat ctgctttatc aggtaatctt tttccttgtc cagcaaaagc
    12181 atttttcccg tatcgacgat attctgaaac ccatcgggaa attgttggac gagagatatt
    12241 gtattcttca gcaattttct tggtagttac ttttttctca aggatatcct gtgaaatctt
    12301 tagtttaaat ttacgatcat atgttttttt caatagagtt cattccttcc ggtaacgtaa
    12361 ctgatagtca agaaaagtgt atcatagccc aacttagtat cagggtttgt caactaaact
    12421 gtggaagttg atagataaga gtttttaaca accaaaattc tcttgactat ttttgaaatc
    12481 agacaaattt tgattggaca catagttgaa taaatctacc gtcgttacgg aaggtaaatc
    12541 gcatgctttt caattctaga ggagaagtcc tcgttgaagt accatggccc agttctggat
    12601 tcgacctcct gcccacttcg tacgtaaata aagtaatttt aggagtgaga ctgtgacaaa
    12661 agttattttt aagctaaaaa cgaattaaaa aacgttaata attaaaatca ttcgttttag
    12721 attctatgaa tcattataga acgctgtaaa atcgatactt tataattata aagaagatgg
    12781 cttcaaattt attgttcgaa atttgaaact atctttttac ttatgtcaca gactccctta
    12841 gttgagttat tctttaattt cgaatttcga gatagct
//
LOCUS       DACDIH010000072        12639 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.072, whole genome shotgun sequence.
ACCESSION   DACDIH010000072 DACDIH010000000
VERSION     DACDIH010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.072"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(53..1429)
                     /locus_tag="IV281_002213"
     CDS             complement(53..1429)
                     /locus_tag="IV281_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8781186.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(1466..2332)
                     /locus_tag="IV281_002214"
     CDS             complement(1466..2332)
                     /locus_tag="IV281_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP8781187.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(2421..3446)
                     /locus_tag="IV281_002215"
     CDS             complement(2421..3446)
                     /locus_tag="IV281_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8781188.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(3464..4138)
                     /locus_tag="IV281_002216"
     CDS             complement(3464..4138)
                     /locus_tag="IV281_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8781189.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(4322..5215)
                     /locus_tag="IV281_002217"
     CDS             complement(4322..5215)
                     /locus_tag="IV281_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8781190.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(5289..6986)
                     /locus_tag="IV281_002218"
     CDS             complement(5289..6986)
                     /locus_tag="IV281_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8781191.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(6979..8292)
                     /locus_tag="IV281_002219"
     CDS             complement(6979..8292)
                     /locus_tag="IV281_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8781192.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(8292..11810)
                     /locus_tag="IV281_002220"
     CDS             complement(8292..11810)
                     /locus_tag="IV281_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP8781193.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(12103..12561)
                     /locus_tag="IV281_002221"
     CDS             complement(12103..12561)
                     /locus_tag="IV281_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8781194.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         3439 a         2911 c         2147 g         4142 t
ORIGIN      
        1 caactgttga tgggcgtttt tcttatcttt cttctaactt ctccattgga gtctatttct
       61 cattcatctt gctgtgtcca tagccatagc caaaataaat gatcagtcca ataaccatta
      121 cgataccgaa cacgatccat gtcacagttt gcagacgaag catcaataca atacaagtca
      181 agatggagac gattggcaat agcggaacga atggcacttt gaattctcca gcttttggtt
      241 tgccaaacat ttttctcaac ttcagtaagc ctagactaac aagcatcaaa taggcgagcg
      301 ccatgatatt cgttagttcc gctaatacat tcaatgggaa aaatccggca aagaatacag
      361 ctgccacacc agcggcataa gtcgcttttt tcggtgtacg atgcttttga tcgatttctg
      421 tcataaactt aggaagtaag ccatccttgc tgacagcaaa cagtaaacga gcaagtgaat
      481 acatcatcga gatcgttacc gtcagtagcg ttaaaattgc ccctacagcg atcaccgcgc
      541 ccactgttcc atgtcctaca tacctcattg caaaagccac tgggtcattg actcctagtt
      601 cagtatacgg aacgattcct gtcaagatca gcgtcacgat gatgtaaagg acagtagcaa
      661 tcagtatcga accaatgatt cctcttggca catctttttg cgggtttttg acttcctcgg
      721 ctgccattgc aacggcatca aatcccaaga aagcaaaaaa cacaagagct gcaccatcta
      781 gcacaccgga aaaaccaaaa ggcatgaatg gctgccagtt atcaggttta acgaaaaata
      841 ttcctacaat gataaacaac gcgatgatac caaatttgac atataccatg gcattattca
      901 aacgtaatgc tttctttgct tcaagcgaca cgatgtaagt gacgaatatc actacgagca
      961 ccgcaatcaa gtcaatgtat gtaccatttt ccggattgta cccgccagtt aatgcttgtg
     1021 gaagttcggc ccctaaactt ttcaaaaaac cttgcatata gccagaccat ccggaagcaa
     1081 cagaggaaac cgctagtaaa aattccccga tcaacaacca ccctgtcagc caggcagcaa
     1141 attcaccaaa tacaacatac agataggcat atggcccacc aatcactggc actcgcgaag
     1201 caaattctgc gaaacttagt ccagacaaaa tgacaacgat tgcagctaag acaaatgaaa
     1261 gtgacagtgc tggaccagca ttttgattgg ccgcaacccc tgtcaccaca aatattcccg
     1321 taccgatgat cgcaccaatt ccaagcatga tcaaatcaga cgtttttagt tcttttttca
     1381 tctcactcgt atgattgaga ttgatctctt tcttacgaaa caaatccatt gaagttcctc
     1441 cccattttag gttggaaaac agcgattaaa ctgttttcat ataccctaaa aggataccac
     1501 ctaaaatgac taaaaggcaa ccaacaataa cataaatgag ctcttttttc gtttttcttt
     1561 cgccaaggat gaaaatccca cctaaagtag agataatgat ccccatttgt gaaagtgaga
     1621 agccaacagc taaaccaagt gatttgactg ttagaagcat gcagatgttt cctaagcccc
     1681 ataataatcc acagatcatg tttttccaca caaactgatc gactttaact ttacggaaag
     1741 caaagaagct tgcgcctaag atcattccga tactttgagg taagataatt gacatcgcat
     1801 ccaaattcgc ccagttgacg atgatggtat atacaccata accaatcgta gagaagatca
     1861 atgctctaaa acccttacca aaatcaagca ttttattatc tgtatctatt tttccttcgt
     1921 catcttgtcg ggcagtcaaa tacgcaccgc ttacaagaag agctaaagca ataataccgt
     1981 atacataatc tttcgtttgt gtccattcat ggaagaaaac ggctcccgca atcgtattca
     2041 aaattaattg aaaaccagta gacaatggta gccctacaga aacacccatg tacttcattc
     2101 catggaactg tccattttgt ccaacactcc aagctaagcc ggataaaaat ccgatcaaga
     2161 aaatccaagg ggtgattaca ggactaacaa cgaaaaagac aactaaagaa aacagaaatg
     2221 caccgatcgt cattcctagt gtttgttgat tggcatcgcc accaatttta ccacttacta
     2281 aaccaatact accccatgcc aacataggga ctaatgctat taaaatttcc ataaccaatc
     2341 tccttttatt tcacatgata agaaacttat cagcgaaaac gttaacaaca caagcacttt
     2401 ttttcgtttc taacgatttt ctaacgtttt tcattataaa aatttaagat tgggaaaaat
     2461 aaagtcattg ttgtcccttt tccttcagtt gattcagccg tcaaatggat acctaatttt
     2521 tcggccagat tttttgctaa ataaagtcct aatcctgttg aatgtgtttt gctaagtcga
     2581 ccatttctcc cagtaaatcc cttatcaaag atccgtttca agtcttccgc tgggataccg
     2641 atccctgaat ctttaactga aaggctgact ccttgcgctt ccttttctat gctcacaatg
     2701 atgtttccgt gatccggcgt atatttgatg gcattactaa gcaattggtt gaagataaaa
     2761 gcgatccatt tagcatcggt caaaacagta tgatcctcac caacgatctc gtagcgaata
     2821 tttttttgga taaaataatt tgcctgactg cgtatcacag gctgaatgat tccttttagt
     2881 ccatattctt ggatgaggta gtcccgcgag aaactgtcga gtcttgcata atacaaaact
     2941 tgttctacgt attcatcgat ttttgataat tcattttcta gtaatgtata cttttgatcg
     3001 tcaatatcga actcgatcga acgaactagc aatttagaag cggaaagtgg cactttgatt
     3061 tcatggaccc atgagtcaat ataatctttt tggtcttgtt gattactgat tgctgattgc
     3121 attacttctt ggtgttcacg gatcagctga ttgatatatt cttctaccag tttttcttct
     3181 tctgtcctcc caccactcaa ataattttgc aaaggggaag ttttttgtat gtcggctaat
     3241 tttccccacc atcttctctt gctcaagtaa ccacccagca gataaaaaat cagaaagacg
     3301 ccctcaatca aagctaaata agcaattgtg ctcaaatcaa ctttgatgtt tggcgccagc
     3361 cacatcacaa agatcgtcaa tgcaataaaa aacagccatc ccaagatcaa tgtccactga
     3421 tctttcaaat atttaaaaaa attcatctta accgctcatc ctttcaggga acgatatatc
     3481 cctgtcctac ttttgtttcg atatatcctt ggatacctgc ctgttcgatt tttcggcgta
     3541 accgattgat gttgactgtc aatgtattgt catctacaaa tcgttcgtca tcccacagtg
     3601 ctcgcagcaa tttttcccga cttaagatct ttccattgtg cttcatcaaa ataaataata
     3661 gacgatattc atttttgctc aaatcaatga tctctccgtt gatttccata cttccgttgt
     3721 caacattcaa cgtaatgcca ttgtggctca tcatttcact agataattct gtgtagttgt
     3781 aagaacggcg aagtaatgca ttgatttttg caatcagtac atcgatttgg aaatgttttg
     3841 tcacaaaatc atctgccccc atgttcatag ccatgatcat atccatattc gtattcctgc
     3901 tggagataaa aatgatcggt actttcgata cttcacgtat tttttgacac caataatagc
     3961 catccaaaac gggaagatta atatctagta atacaagctg tggttcttct ctttcaaagt
     4021 ctgagaacac ttgattgaaa tcggtcgttc caaaggtttc aaattgccat ttctcaagtt
     4081 cctccatgat caattgtcga atgatctctt cgtcttcgac taccatgatt tttgccataa
     4141 aaaatccctc catctttctt cttgctacta caatatcaaa gaagcccttg tgaaacaagt
     4201 tggcttacct ggaaaatgtc attttagcaa acgttaaaaa aaactggaaa tatgcgattt
     4261 aatcgcgttt gtctcttcca agcagcttaa atttattatg ttattcttaa taattggtct
     4321 attatctgtt atttttcttt cgttgttttt gtcttttaaa ttttcgataa caccaataac
     4381 cgaaaagcgt tagcactagc aatataccta gaaggagtat aagtaatcga taaagatggt
     4441 atttttttgt ctgttctatc tttgaagaga cttcttctgt aaagggaact cgtcttcctg
     4501 tcactagtaa tcggtgagtg ttcacggtgt aaggtgtaca agtgataagt gtgactagat
     4561 cctgcttttc tctaattgct aatgtatttg tttttgttgg aaggacaaca attttctcaa
     4621 cgacttcata agcgagcatc cgaccataca cttcaagata gaatcggtca tcttgctcca
     4681 tttcttcaag atccgtaaag agttttttct cagcaatacc gctatgggcg gtaatcacgg
     4741 aatgtgtact ctctcctcca gttggaaaag aggtgccttg caggactgtc gctcctctat
     4801 ctaacaaaag atcattcgtt tcatcaaata aaggcaagct aacattgatt ttcggaatat
     4861 agatggctcc gatcatatgt tgttcgtaat attcttttcc tggattttgg acatttctaa
     4921 ccgcttgctc aaaaggatct ttgacttgtc ccattcctgg gattactgat gtatgagcaa
     4981 gcgtctgatt tttcttttca tgcactgcta attgttgctc atgtttttct tctttatcgc
     5041 gttcaagctg cttttgataa ttctcgatcc ttctttgatc caaataattg ctgagagcat
     5101 ctgctaaaaa tggatatgta aaaactgcag ctccggcaag aaacatcact gccataaata
     5161 attgtaataa aagactgatt tgggtttgag gtctattacc tattttcttt ttcattttct
     5221 tcaccttctt atttaaaaaa actgcagcaa cctttataaa accgattgct gcagcttatt
     5281 tttgttgctt atacttcaga cttcatttta gatttacggt accagctgta cgctccaatc
     5341 atcaaactta ttccgattag taagaatgcc aagagcccgt tcccaccagt agcaggaagt
     5401 gacccgcgtt tcatattcgg tactctttct ggccctggat tttctactcc tacgatctga
     5461 atttcttcat tatagcttcc ataagagatg ataaattcag taaaggctgc ctcacctgtt
     5521 ggaagtacat aaccttctgg cgccatggtc tctttcattt gataggttcc ataatctaac
     5581 ccttgaacct caaagtatcc atttccatct gcgacaaatt ctgttgcttg tccgcttgtt
     5641 gcccactcaa ttctatctgc tcctgattca taaacagcta aaggcgactc ccctttataa
     5701 aatattgcgt attcagaacg gtcttcattc cacaattcga atcttgcatc tttaagcaat
     5761 tcactgcttg atgcatcata tttttcaaac tttcttccac ctgttgtgac tttctctggc
     5821 ggctctactc cttcttcata atcaaattca tcatttcgtc cgatttcaat aaccgctgtg
     5881 ttgttatgga aatcatccgc ttctagttct gtggaaacct tcaaattata cgtgatcgtt
     5941 aaatctcctc cacgtgcatc tgctaatcta cctaaagtat ctactttggc tactccattt
     6001 tcttctgtca aaatcgtcac gacaaacccg ttgcttacta cttctactat ataatcttgt
     6061 ccttttgtta aaatcgtctc gccggctcgc acttctattt cctgatcata ataagttaga
     6121 ccaactgtag gcgtatctgt gattttgtaa tttttgataa cagcgcttcc gttttcaagg
     6181 acagaatcga tgaaataagg aataggtgct gtgatcgtgt aagaagccgt ttttcctctt
     6241 tctagatcaa tataagaaat cgtttcttgt tctccagctt cattaataat cgttacttgt
     6301 ctaaggtcgc tttcctctac attcatctgt ttatctgctt ctctgatttc gtttttcgga
     6361 tacaaatgca catctgtatt gtatgtgtct ttccatgtag aaccatccca cacgccatcc
     6421 accatatcag gcatcatcac tggcatcata acaaccattg gagacgcgat tctggaaata
     6481 tgagctggcg aataagtttc taagaaaaga tagactttat ctctagaaga aggtgcttct
     6541 tccgtcactt ggacggtaga gaataccgct tctccagctt cattcgttgt ttgtttgtca
     6601 atacttgtca gtcgatattt taaaaaccaa ctttctgtgt gccgtttttg aatccattca
     6661 atcgcttcgg cagaattaag accgtcttct cgttccggtg ttaaaggatt atctcgaatc
     6721 aaatcgtaat atacttccgt tacgtcaaac aaatcaaaat cgactcctgg aatattttct
     6781 tcaccaaaat ccgtagaaac caatccgcta ttcggtcgtt cttccggaac agatgagaaa
     6841 ccttttttgt gtaatgtaat cgtcacttca cttggttttt gtgcgacttc ttcggctttg
     6901 actagttttc cgttcccgaa aatccctatt cccgcagtaa cgagtaacag tacactcatt
     6961 cccgtgacaa tcattttttt attcattttt ttctccccgt tcttttttta ttaataaaac
     7021 agcacctaac aataaaagaa ttcctagtaa agaaatcaac gttcttgctt cattcgtttg
     7081 cgggaatacg gaccattttc ctgattgacc tggattgctt ggaccactcg gctctttcgg
     7141 tttgtccgtt gtttctttta cggtctcgtt ataaacacgt ggtgttgcgg attcataggc
     7201 agagactgga actttcccgg attctaattc agccatctcc tgtccgttaa ctactacata
     7261 ttgtggttgg ccttttgcat ccacccaaaa ttcagggatg atgacctcga ttctcttact
     7321 tgcttcatcc atctcgtaac cttccacacc ttttaattct tcaaaataat aggtgccaga
     7381 aggcaagaaa cgttgtcctg tcgtaacaag gccctcttta tccgaaatga atttgcttac
     7441 atttttgtca ttcaaaggat cattgtctgc tggtttgatc cattgatttt tcaagtcatt
     7501 agcaggagcc atatctaaat aatatttccc cccttctatc atctgatata gagcaaaaac
     7561 agcaccttct aatggttttc ctttttcagt tgttcctctt tgacgtccat acttgaagaa
     7621 gtatggatct cttagataac cgacattttt aggatagata tgaatctctt gcagttcctc
     7681 ggttgattct gtcgggtgat aaattggcaa aatagcagca attgggatag ctgtctgatc
     7741 gatatcaaca tccaatccaa cttctggatc aatcgattct tcgaaaatca aataaactgc
     7801 atcccgatct tggtttttct taggtgcttt gatacgtgca atcccgtctc ctaatccgcc
     7861 aaccgtatct ttcccagttg ttaccgaagt ccctatttgg acaagccttt cttctgtgat
     7921 tagctgacgg atattgtttc tgttcgtttc agcgatttgc tggacgaatt ctttaggagt
     7981 catgttttca gcagcttttt tttcatgata ataatcggtc atgtcgtaaa tggcaaatgt
     8041 cgctccattt agtggcaccg tctgtgaaat gatatctgct gtttcccctg cttcctcaga
     8101 gatagccaag ccatcatttt catggtattc aaattgatcg ttataatcga tatctcgaat
     8161 catccgttta tgcaacacga attccacgac atcttcccct gattcttctg ccgacatcgc
     8221 atgtggaaga aggactgctg caagcggtag taaaacaagt aattgaagca tcaaacacaa
     8281 cagctttttc attaccttgc tccctccttt ttagattgtc tgacaaacaa accataggct
     8341 gctcctgcaa gcgccatgaa gaatgcagca agcaggatat agaataatcg tccggatcct
     8401 cctgtagcag gcaacggatt ttcaggatcg ttcccgacac taagctgtaa tctgattcga
     8461 tttgtctcca aaagttcata ttctaactct tgctgtagat caggcgtctc tgcttcttcg
     8521 tttggataat acgaaatctc aacttttagt gtacccgctt cttttcctgg aactacccga
     8581 tcattttcta tgtcaggttc ttctcgataa ctaatttcta aaataaagta acctggaagt
     8641 tttctatagc tgtcaggtga cttcacttcc ttgatccgat agaccccttc caacaagtct
     8701 gaagcattga atagggaaga atcgtttgga tctggtgcca agcctccctc gatgatttca
     8761 tactctcctg cactattttg tttctcaatt gaaaattctg cgccttcaag acgtcggcta
     8821 ttcatactgt caattttcaa aagttccaaa tcgattggcg ctagttcatc ttctaaggtt
     8881 acttgtagta caccatcgat gatttcatag ccttccggca agggagcagt ttcgtcttcg
     8941 atgaccgtat cttcaatgat aaacttacct tcttcattca gtgcaaactg gaacacttga
     9001 tcagatagcc gatacccatt tggtgcgaca gattccgaga cctcatattc accaatagac
     9061 agatgcagag gttggtcgta atttaaatcg acatactttt ctactcctac ggcacgagaa
     9121 tagaagtatc cgatcccagg caaacgtttc gattcttctg gttctaacgg attaccagtg
     9181 tatgctcgat acagcgggaa ggaagaatcc cccactttcc tgatatcgaa acctgcacca
     9241 tctaatgtat ggccttcatg gtctaatttt ctaactcgga tctcccgata atcaaacgta
     9301 ttgtaagcag tgaatgctac ccataaacga tcctcttcat ctgttgtata cgtgagatat
     9361 tgccaatttt cttcacctgt ttgcttgagt tggatagtcc ctatagcatt atcgtaaagt
     9421 cgcaagatcc atgtgtcatt ttcatcaggt tctttgaaac cttctgggtt ctggctctca
     9481 atgattttgt attctgcata gttggtagtt tcttcactat ccttataaac aaatgaatct
     9541 ggctctccga ttggatgaat aactttctca ccatccacga ttacatattt cccgtcttca
     9601 tctactgaat agaacgtgag tctccctttc tcttcctcta cttcgttcac accagaaagc
     9661 cctttcgcta ctggatatcc gatttccagt ccgttaccat ctgtttcata aagttcaaac
     9721 tctgcgccat tgattggcat attactgcct tcaattagtt tattgatatt catgtggatt
     9781 ggcttgaatt catttcgcat ttccagtttg acatacaact catcatactc tgtgccttct
     9841 atgttaggct cttgtacaac agaaagcttg tgatattctc cttcataata ggaattcgga
     9901 tctttacctt ccggtccgcc gactctcgaa ccagatgtat attcatctac aagctcgtag
     9961 tgacctaaca tctcatcttc gttggctgca tattttttga tttggaattg atacgtctca
    10021 tctactaact gataacgtgg tggtccagcg atctctctga tccaaaatat gtcgctgact
    10081 gctccagttt catctcctaa aatcggcatc ttcgtttcaa agatcgctat tccgtccgtg
    10141 gtagttttac ttgtttcgat tggttcggct tccccactct ctggattttg gtcatataat
    10201 gcaaattcga ctccagccaa cggctcgcca ttttgaccct tcttgctgac ttggaaatta
    10261 cggcctttat gttcgtaacg aatggaagaa gatgggaaat acatccgtcc agtagtgata
    10321 tcattctcat aatcctccag gttctgaata taggccatat tgttcactgt gcaaaaattt
    10381 ccgtcttgag ctcccgcatc aagttttact tggtacttga tcctgaccat ttccccttca
    10441 cctaaagaaa tcggagaaaa tttataagct tggttttccc gtccgacata cgttgtcgga
    10501 gtcgccacta cttctggtgg ggcttgtcca tgacgttctt catcccatat ttctggctct
    10561 tggtacttca tgccgttttc tcctggatct agatggtaaa actgaatatc tgccgatctg
    10621 ccagctccaa atggtgagac attaaaacca gtttcatcat aaagtgtggc ttgctggatc
    10681 gttctcttga aggtagaaga gatatcctcc aaggctgtaa caatgtcatt cttgttgttc
    10741 gcattgtaat atcgcggtgt tacgccatct tcttctaaat cagcaatatt tttcaataca
    10801 tttcgtccaa tagcatctag tcgttgtctc ccgctaacgt ttccatcgat tcctaaacca
    10861 atggagaaga tacttggcgg gttctcttga tacttgtttt tcagccaatg atgataagca
    10921 actgtgttga tcgcagatga cttttcttgc gaagtaggac tgccattcgt attgttatta
    10981 gaagaaccat caccaaccca gtgagtcgct ttattatctc cttcttttac ctctccataa
    11041 cgatagtgta catttacttc atcttctgga cggttaaagt cagatgcata ggtaactgga
    11101 tgagtaaacc ctgcattaga agtattccca gaaaactcct caaaatttct gaacaaaacg
    11161 tcattatctt cagcaatctt atcttccatc acagtccagt tgctccaagc tccattagtg
    11221 ctcgtacgat aacttgactg aatcggtgca taactgaagg tcggtgtccc atctcccaca
    11281 acgattagga ttttttctgg atttctattt tctccgccat tgtctttata caatgtttca
    11341 tagccatttt taagccctag tgtaagcgga gttcctccga ttggtgtacg atttaatgct
    11401 gtattctcac ttcccgtcaa acgattgata tcatttgtca atggaaagat gtccgaactg
    11461 atttgctcat ggttattgat agctgtacta ttatggtaaa aattcaccat accgattcga
    11521 atatccagac tcgggtcaga gagcagatct ttactcattt cgtttactgc ctcaatcaac
    11581 gcagcatttt tagtttgact attggtccca gcggttaatt cactcatact cgctgactta
    11641 tctagaacaa aaacgatatc gattggactg atttcctttt tctcgccccc gatgacatct
    11701 agatagacat tgtaaagggt tgttgcatct tcaggatgta gatcagatgc tgtgctgctt
    11761 ggcagaacag gttctaccca ttttttgatg tacgccccgt tataatccaa gtaactgttt
    11821 tcaaaattcc gttcatttcc ccaaagctga acggtttgag ctggaacgtt tctatcttct
    11881 tcgcctgcag ttgatccatt attagtagca tagtcgtaat ttcgccagtt cgctcccccg
    11941 tttgtataat cactatcatc gatcaagttc tctccgtcat atggcgagcc tttgtctcct
    12001 tgatagcgtt cattgctttg tgtccaattt cctaacactt ttgtatagtc attcgttgta
    12061 aacttcatct attctttcat caaccaccac ttgaagaggt agttaccctt cgttcacatc
    12121 ggggatcttt tctgtttcaa aaatcaatgt gccggtttct gcttcttttg aataaacttt
    12181 tccgacatac cattcttgac catcgatcgt ctcgactttt aggtcttctc catcttcatc
    12241 ataggaggat agacctgttc cacgaacgtt ttttacggta ccaatcccgg cggctgccgc
    12301 atcgattgct aaacgtattc taccttcatt ggcactttcc tttttttcaa aagccagatg
    12361 ccattcaagg gtttcttctt tttcaatata cttcccttca atttttgcat agtcctcatc
    12421 taagaatacc tcatcctcct ttgtttctgt tgactcagca gctacatcca gggtcgtcat
    12481 atgatagact gactggaaat atggtccaaa tagcaaactg acagttacta actgcaaaag
    12541 cttcaatttt aaccctttca catccttata ctcctttcaa aaatatttgt cttctttcac
    12601 aacaatcaac aaaacaatgc ccatctaatt ataatttaa
//
LOCUS       DACDIH010000073        12033 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDIH010000073 DACDIH010000000
VERSION     DACDIH010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.073"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1387)
                     /locus_tag="IV281_002222"
     CDS             complement(<1..1387)
                     /locus_tag="IV281_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8781195.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQ"
     gene            complement(1581..3203)
                     /locus_tag="IV281_002223"
     CDS             complement(1581..3203)
                     /locus_tag="IV281_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8781196.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(3531..4844)
                     /locus_tag="IV281_002224"
     CDS             complement(3531..4844)
                     /locus_tag="IV281_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8781197.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(5089..5313)
                     /locus_tag="IV281_002225"
     CDS             complement(5089..5313)
                     /locus_tag="IV281_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781198.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(5333..5701)
                     /locus_tag="IV281_002226"
     CDS             complement(5333..5701)
                     /locus_tag="IV281_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8781199.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(5897..6607)
                     /locus_tag="IV281_002227"
     CDS             complement(5897..6607)
                     /locus_tag="IV281_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781200.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(6625..7122)
                     /locus_tag="IV281_002228"
     CDS             complement(6625..7122)
                     /locus_tag="IV281_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8781201.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(7159..7611)
                     /locus_tag="IV281_002229"
     CDS             complement(7159..7611)
                     /locus_tag="IV281_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8781202.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(7746..8351)
                     /locus_tag="IV281_002230"
     CDS             complement(7746..8351)
                     /locus_tag="IV281_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8781203.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(8801..9421)
                     /locus_tag="IV281_002231"
     CDS             complement(8801..9421)
                     /locus_tag="IV281_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8781204.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(9423..10292)
                     /locus_tag="IV281_002232"
     CDS             complement(9423..10292)
                     /locus_tag="IV281_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP8781205.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(10315..10722)
                     /locus_tag="IV281_002233"
     CDS             complement(10315..10722)
                     /locus_tag="IV281_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8781206.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(10741..12030)
                     /locus_tag="IV281_002234"
     CDS             complement(10741..12030)
                     /locus_tag="IV281_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8781207.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT         3450 a         2600 c         1980 g         4003 t
ORIGIN      
        1 tttgtctatc cacataaata aagccatatc gcttcctcat ttcccctgtt cctgccgata
       61 cgatatcgat tggtccccac cataagtaac caatgatttg gcaaccatct tttattgcct
      121 cagcaatttc ctgaagatgt atttttagat actcacaacg ttttggatcg tggattcgat
      181 tcaattcatc tggtgcttca tctagtccaa tgccattttc aactatgaat aatggtaatt
      241 cataacggtc tgtcaattca ttcatgacat aacgaaaccc tagaggatca attggccaac
      301 tccaaggttc gggagatttt tccttcaaaa agggattatc gaaaccattt gctccgccag
      361 tatctacgcg catttctgct tcttttttat agacagcgct acgataataa ctaaatgcaa
      421 tataatctgc cgtgttagct tgcaagcact gttcgtctcc atctaacatt actggtgcac
      481 agtcttcttc cttccatgtc ctgtaaatat aagctggata atgccctcgg cacatgacat
      541 cggagaaaaa ccatgaattg cgtttaaatt gcagtgaacc aaatacattc tctggatcac
      601 agtcatatgc ataagtcgct aatgcagaaa agctacacat aatacccatc ttagcatttg
      661 gatcgatttt ctttaataat tgagtagcac gggcgttcgc tagaaacata tgatgacagg
      721 cttgataaat cacttttttt gttagtgtag caatgggttc aagtttatct ctagcaggga
      781 atgtaaccaa tccagcagct gcataaggca tacgaaaagc attattgatc tcattaaagg
      841 tcatccaata tttcactttc ccttggtagc gttcaaagac ggtctgcgca taaaattcaa
      901 aaaagtcgat taattttcga ttttgccacc ccccatactc tcctaccaat gctaaggggg
      961 tttcataatg actaagagta attactggct cgatatcata tcgatttaat gtattgatta
     1021 gtctgtcata aaatacgaga ccagcctcat ttggcttctg ttcgtctcct cttggaaata
     1081 ttcgtgccca tgagatgctt gttcgaaaag cttttaaacc taactccttc agtaaacgaa
     1141 tatcctcttc aaatcgatga taaaaatcaa tcgcttcatg agaaagataa tggagtgatt
     1201 cgtctaaagc aatctcccaa atcttttttt cttcattcca ctgaatacta ggatgctggt
     1261 tcatgatccc gataagcgta tctgtcacat taggtaattt gccgtcttcc aaccatgcac
     1321 cttcacattg attagcagct actgctccgc cccataaaaa attttctgga aatatttcct
     1381 gatccataac tatctctcct cgctatcaac aatttcttac ttgaagtata gctcgattca
     1441 tcaaaacaag atattctaac tatttcggca gaagagaaga aaaaaattga atgctactct
     1501 tcatttttaa tatttatata taacaaatac ataagacgat ccccgaaaga aaacactcgt
     1561 taaccatcta agcaaacgat ttattttact tccaacacaa atgcttcata aggtcgtaaa
     1621 tcaactttgc cgtctggtaa ctgtttcaga ggataatttg acacaataac ccttaaagct
     1681 tcacgtggaa tagtgaacgt ctgcttttca ctggacatat tacaaataac gagccattgc
     1741 tggtcttcat atatgcgaga ataggcaaag acatgctcag gcgtcttttc cactaattca
     1801 tagtctcccc agacgatgat gtcatgtttt ctacgcaaat cgatcaattt cttgtacata
     1861 taaaaaacag aatcttgatc tgctaacgct tcttctgcat tgatttcttt ataatttgga
     1921 ttgactcgaa gccatggaat tccagtagta aacccagcat gttcacgggc gttccattgc
     1981 ataggcgtcc tagcattgtc tcttcctttc gccttgattg attccaagat ttcctctttt
     2041 gtaaatccag aagaaatcct ttcgttatac atattgattg tctcaatatc ttttgcttcc
     2101 gcgatatcat cgattgggca attcgtcatg cctaactctt ctccttgata gatataaggg
     2161 gtgcctttca tcatatggag caagatagca aacatcttgg cactttcttt tcgatacaca
     2221 tcgtcatttc cccaacgaga aacaattcgc ggaagatcgt gattattcca aaataaacta
     2281 ttccatcctt catcacctaa agatgtttgc catttggata atactttttt cagttcagag
     2341 atactcaagg gtttcaaatc ccatttgtct ttgccttctt gctgatctaa tcctatgtgt
     2401 tcgaattgga agaccatgga taattcattt cttttaggat tggaataaag tttagcgatt
     2461 tcggtagttg ctccccatgt ttcgccaaca gtcatcagat ttttgtcacc aaaagttgct
     2521 tgattcattt cctgaagata ctcatgcagt ttaggtccat tacttgtgat ttctttatcc
     2581 ggttctttgc caatcatatc gatcacgtcc atacgaaagc ccccaatacc tttatcgatc
     2641 cagaaattca tcatttgata aatctcttgg cgaacctgtt tatttttcca attcaaatca
     2701 ggttgacgct tgctatacaa gtgcagaaaa tattgccccg ttttttcatc caactgccaa
     2761 gcagaaccac tgaatattga gcgcaatcta ttaggctctt ttccattgac cggatcacgc
     2821 caaatataat agtcccgata aggattttct ttatttttac gggcctcaat gaaccacgga
     2881 tgttcatcag aagtatgatt caccaccaga tccatgatga tacggatatt tcttttcttt
     2941 gcttcttcaa tcagttcttc catttcttcc atcgtaccga attctgccat gattgcttga
     3001 taattactga tatcataccc attgtcatca ttcggagact cgtatactgg agaaagccaa
     3061 attgcatcaa tacctaaata cgccagataa tccaacctgg aaataatccc ccgcaaatct
     3121 ccaataccat cttgatttga atcttgaaaa cttcttggat agatttgata aacaactgaa
     3181 tggtgccacc atttttgttc catgattttc ctcctataaa tggatgtttc tattttttgc
     3241 ttaatcattt attttagtct ctctcactaa aaattgtaac gctttcaaat agctattgca
     3301 ccttgttatc ggtaacagaa taaaacaaaa aaacagctga ggaaattccc tcaactgttt
     3361 tttgaaagga ttgatttatg cagggaagaa tgctgatttc acaacttctt tataaacggt
     3421 gttcactcag ctgtcccttg gtattagctc aaaaaacggc aaaatataag aaactatttt
     3481 ttcgtttttt tctctaatac tcaggccaga acgctgattt cacaacctct ttatttcaca
     3541 ttcaatatcc acatgaatcc cacaaataca aagaacagac catacatgat tggagatact
     3601 tcttttcctc ttttagctgc taacatggta attggataga agataaaacc taatgcaatc
     3661 ccatctgaaa tactataagt caaaggcatg ccgatcaaga tcaagaatga agggatcgca
     3721 atctctaagt ttccccattc gatttgtcgc aaagattgtg ccatcaatac tcctaccata
     3781 atcaatgctg gtgcagttac ttgtgaagta acaactgata gtaatggaga aaagaacaag
     3841 cctaacaaga agaaaatccc tgtagtaata gctgtgatac ctgaacgtcc tccaacggcg
     3901 ataccagctg atgattctac aaatgcacca acaggagaag taccaaataa ggaacccgca
     3961 agcatagctg ttgaatcaga agctaaagct ttccctacac gaggcatctt gttgtctttc
     4021 atgaaacctg cttggtttgc taatccaact aaagttcctg ctgtatcaaa gaaagtcaca
     4081 agcaagaatg tcagcactac gacccacatt tgaagagaat tgatgtcccc tacatgtttt
     4141 aatgcaacta aaaatgttgg tttcaaactt ggtgctgcgg caacaatttc agaaggcatt
     4201 ggaatcaaac ctgtgaccag acctaaaatc gttgtagctg ccatcccgat gaagatgcca
     4261 cctggtacac gccgaacaag caagatagca gttacgacta atccaaaaat cgtcaaccaa
     4321 gtagaaccaa cgttgagtga tcctaatcct actaatgtag attcattcga aacgatgatc
     4381 ccaccttcgc tcagaccaag gaaagcgata aatagaccga taccgccaga aatcgcatat
     4441 ttcaaatctg ctgggatcgc atcaatgatc atttcccgca gcttgaagat tgtgatcaaa
     4501 ataaaaatca aagaagcaac gaatacgccg gctaatgctg tttcccatgg aatccccatt
     4561 cctaaacaaa cagaataagc aaaaaaagca ttgatcccaa gagcaggtgc cgttccaatt
     4621 ggataacggg ctaatacccc cattaaaaca cagccgatcg cactggctaa tgcagtcgca
     4681 gtaaacactg ctccttcatc cattccagaa gcacctaata cagttggatt tacaaataag
     4741 atataagcca ttgagataaa tgttgtaaat cctgccagta cctcacgttt taccgtggtg
     4801 ttcaattttt ctatttcgaa gtaggaaagt attttttcct tcatcttcca tccccctaaa
     4861 tccgaacatt ataaataaag tccgttaaaa tttcgattta ttaataaaac aacgtgtttt
     4921 atcatatact aaaacaaaac ataagtaaac aaaaaattcg gattttaagc aaaatccgaa
     4981 cgttccatct gattaaatcc ctttatctca aagttcatga acattcactt tggattacaa
     5041 gaatttcttt attgtgttaa taaagtttct tcattctagt ctcaaaaatc aatagtgtga
     5101 tttgatttta ccatttcgtt tctgttcttc tttgatgaat tcttttgtat cgacaggatt
     5161 cagattattt agtccgccaa aacctgtatc gaccattttt ccccagcctt ttgtcttctt
     5221 ttttcctgat ttgagattct ttgctcgaag cttctcacta tgttttgctc gccgatactt
     5281 caaataacct tctaatcttc ccattagatc cactgttttt cctccttttt attcaatcag
     5341 acgactcagc ttttcctata acaaaataat tgccttcagg gtcagaaaaa ttgaaagtca
     5401 agatgccatt gttatctgtg atttctcctg gttcttccat gttctcgtag atagtttcaa
     5461 attcatccgt aaaaaatact aaagaaggcg gtggtcctaa tacctctggt gaaaacttct
     5521 gcacaaatac ttttgggaat acgcctaatt ctaattcttt tgagatgctg agtacaatac
     5581 tcttaaattc ttctggcaat tcgattgttt cagaaatttc tgcaccaatt ttttctacaa
     5641 agaatcggca aatcatctct acgtcattga cataaagcat cacacgtgtt ttttgtatca
     5701 tcatttgacc cttctttcta tttgattttc tttcctgtct ataaactgat tccattatat
     5761 cacgaaagcg caaacacctt gtgtttgata ctattcaaga aaaataaagc aatagtttct
     5821 aggattgctt tcctccacaa acttttttta caaaaaaaaa acggcaatga atgccgctct
     5881 tcttatttcg attcttttat aattctgctt ctgattcatc tttggttagt gtgtgtctaa
     5941 cactagttac gccaaatgtt tgatcgattt tttccttcaa cgcttcgatt gcctgtgcgc
     6001 cacgtgccgc ttgttcagca ttgatggatt caatgaccag caccgctgct tgtgtttctt
     6061 ccgtcagcat cgttctttgg gcttcacgcc agttgagaca gcgacagatc gctccttgtt
     6121 catcatagta gatgatttct tccggaagtg ccggagcatc ggaatctgca cctaaaggca
     6181 ggaatgattc gccaccttgg gcttttccta aatgcagatt tcctgcgatc ttttcgatat
     6241 cttctccgcc gcaaggtacg ccaaaagaca aggaaatact attatagata tctacaagcg
     6301 gattgattgg gaaaaattcc cgttcttgat ttgcacgttt cagtaaagct tcaatagaag
     6361 aacgtgcacc ttttttagtc ttgaatgtgg taaatgcctg acgccactga gcaatgactt
     6421 cgttttggct gaatgtttct tctgtaaggt aatttcttgc ttcttttgct gcctcattca
     6481 ataattgatg atacgtttct tcactttctt ttgttacgtg gttatcgata ccatttacgg
     6541 ataagatttc gatggttgct tcaggaaata cttcccagaa attggtatcc actattactt
     6601 ttttcatctg ttcacacctc ttgattagaa taattgcttg atgaatagat agtctgtttg
     6661 gggatcatct cccatataaa aaacatgttc gctgtatttg ataaatcctt ttttacgata
     6721 aaaagcgagc gcaacatcgt tgtgttccca aacaccgagc caaatggctg ttttttgctt
     6781 ttcttttgct ttttcaatag ctgtatctag taaaaatgta ccgatacctt gatgtttaaa
     6841 tttttttcgt acatagattc gctcgatttc taaaaaatga tcagccattt cctcagattg
     6901 actcgttcct gtatttagtt tcaggtaacc cgcaggctct tgatcgatac tcactaggaa
     6961 aaaatcggtt tctttatctt ttagctcttc tgtaagtttt tctagtgcat aagcttgtgt
     7021 caagtaatca gctaaatgct ccggtgaatt atcttttcca aatgtatctg taaatgtttc
     7081 gatactaatt tgttgtagta agtctacatc tgaaggcgtc atttttcgaa tttcgtattt
     7141 attcacggct tcttcctttc aatagatcct tttattgcct tttttgacaa aattccaatc
     7201 ttcgctaaca ttcttgcata ctttatttaa aagatattcc aattgttttg cttcttcgtc
     7261 agaaagacca tttaatgcga ctgcattgga gtagttgttt tctcgaatga tgaaaggata
     7321 gatttctgct ccttttttag tcggatagag tttcttgatt tttttatttt ctttatcttc
     7381 caaacgttcg atcatcccat tggattctaa tttcttgatt gctcgggcag tcgttgtacg
     7441 atctactttg atcatttctg ctagtttttc ttggatgatt ccaggatttt cgcaaatacg
     7501 tactaaataa agatactgtc ctcttgttaa atcgacttct ttgaattcga tattgctaat
     7561 agaatctagc gctcgggcaa tcattccgat gtctcgaagg atttcagcca tttcatccca
     7621 cctttatttt gtttgtaaga ctagttcacc tctttttgtt gcaaatgcaa ctattttcaa
     7681 taaaaaaatc aagttctttt ttaataacta gtaaagtata ttcttttctt cttttacgaa
     7741 aaagctcaat cacaatagat ctggtttttg ttttctaaaa taagtaactg tttcttcgtt
     7801 gttcgctttt ctgatagctt tggcaactct tctatactaa aaaatcccat gttggaagtt
     7861 tcattattct caataaactt ggcctcttca ccgtggatcg cacaagcaaa aacaagttta
     7921 taatactgaa aaagctgtgg aatatctttt tgtttattcg tatcaaaaac tgctcgcaac
     7981 tgagtagttt ctactgttaa tcctgtttct tcatagactt ctttacgcac attttcttca
     8041 ggagacaatc ctatttcagc aaatccacca ggaagtgacc attctttcgt ccgtttatct
     8101 tcgattagta ggaccttgcc atcttttttg atataggcac gaacatctac ttttggtgtt
     8161 ggatgtcctt ctccaatcgt tgtgagcgct tttatttctt cgaaagtttc ttttcctatt
     8221 tcactgatca gttccaatga aatcgtccgg atctcctcaa aacgctcttt atcaaataca
     8281 tcttttccat aatatagacc agcttgagaa agtgcgagta gtcttttgta ttgagccaac
     8341 ttcttgttca ttctcttccc ttctttttta gcaaatgtat gactatgtaa tcaagtatag
     8401 aagactggtt taattacggc aaataaatat aatgaaatct gcaataaata aaagaatgtt
     8461 aaaaaactcg agaacaaaag acattcgact tctctggggc tgtgaataaa tggcgttcaa
     8521 aagcttgcat ctgcggtaat aagccccacc aaacgaaaaa tatgacgagc tattcttgtc
     8581 tggctgttct tattaattga ggcaggacag ctttttcaca acctccccac taaacggtgt
     8641 tctcctacca actcctcggt cacaagtcac aatattcatc aaacatgaga agctgttttt
     8701 ttgaaattgc tccttgtgac tcggagctga acgacaggct cacgacctct cggtaaacag
     8761 cgttcaaaag ccaatttttc gatattaagc catttctttg ttacttattg tcattgattt
     8821 ctttttctaa tttatccaat agtggagtgt aatccacttg agtatagtct gttacatcaa
     8881 attcatatag tgttcctaac tggaatttgt catattttcg gtcggcgatg atctttttga
     8941 atgcttcttt cgtaatatga ttggtccctt tcgttcgatc ttccaatacg tcaccatagt
     9001 gataatgatc catgatatag ctcaaatgac gatctgattc acgatcacgt ttgtaccgac
     9061 gttgccataa cacttcaaag tctgctgtta ggcgaatcgt gatcacctga tatccgtatc
     9121 tatctgctaa ttcttggagt cgtgcttttt gcttatcact aaaaggatac tcggacacaa
     9181 tcactttctt tcctgcttcc atatacaaat caagtgcttt gtaatagaaa ggataaactt
     9241 tcgtcctttc ttgttccgcc cgttcttcca aattgttaaa cccgatggat tctgcatagt
     9301 attccttgat ttcatcgagc gtgatggcat acatatctgg aaatcgttcc cgtagtttat
     9361 tcataagata tgtttttcct gtaccaggac taccagccag aagaatgaaa tactttttca
     9421 tctcatttat ttcctaagtt aattggactt tccaagttca taaacagtcc aatcttatgc
     9481 ttgacaattt cttttactgc ttcatttgct ggaatcacat tatggcgaag agacttgttg
     9541 atttctgttt cactaaaggt ttctttagct actttcgtcc agttgactaa aagctccgtt
     9601 ccaacattga atttgcggat cccgttattg atcaattcag gataatcttc ttctttcaca
     9661 cctgttcctc catggataac tagggggaca tcaactactg catcgatttc tttcagcaag
     9721 ggaatgttta cttctgtttt tgatttgaat tgtccatgat tcgtaccgat agcaatagct
     9781 aatgcatcca cgcctgtagc ttctacaaat ttcactgcgt cttccggtct tgtatatacc
     9841 ttgtcatttt cagcgacatg gattccttct tctgtaccgc cgattgttcc aagttctcct
     9901 tctacggaaa caccgtgcgc atgggcatat tctactactg cttttgtctt caggatattt
     9961 tctttgaaag gcagatggga cccatcatac atgacagaag tatatccgct gtcgattgct
    10021 tctttgatat catcaaagtc tcgggcgtgg tctaagtgca agactgcatc aaccatttct
    10081 tcttctgcaa tagctgtcat tacattgact aacacatgca tgccgatata tttagcggta
    10141 tcgacacttg tttgaataat gatcggcgaa cccatttctt ttgctgcgcc tagcatagca
    10201 ggaagcattt ctaggttatg cgtattaaaa gctccaactg taaaattcaa ctcttctgct
    10261 gtttttgtta cttctttcaa tgttgtatac atcttttatt gccaccttat ctttttattt
    10321 gtttcctttg atcgttactt gttttgcaat ttgtcgcatc tcatccattt tgcccatgta
    10381 gtaatcgatc ccttgatcat caccagcacg agcagcatct gcacgcttct ggttatagag
    10441 gctcatcagt tttttatacc catctccgat cgtctgttta ttttccaaac tcttttctaa
    10501 atattggatg gcttgatcgt tcacatacaa ttcagaaaat ttaagtccaa cttgttcata
    10561 aagtttcgct aatttatgct tatcttgggt agtggaaatc tcttgtttca attgtgtaat
    10621 ctctgattga atcttttcgg tcgcttctgg cgatagggct ttttcttctt ttgccagaac
    10681 ttttttcggt tctttttctt ttttcttaaa aaatccaaac atcattttcc ctactttcaa
    10741 ttaaaatgct ttcaaaactg ggcttaagat ccaggcatat aagagtgctg cggctactgt
    10801 atctacatcg gttgctgtag cattgttgaa tcccatcgaa ttaaagatcg ttactagcaa
    10861 agcgggcaat aatgtaataa agaaaccatg agcaataccg ccaatgatcg ctcctcggcg
    10921 tccgccaact gcgttgccaa atatccctgc tgtccctccg gcaaagaaat tggttagcat
    10981 accaggtaaa atcatcgcta agccgaacat gggcaaagta aacatggcaa taatcgtacc
    11041 aatcgttgtc gtaatgaaac ctaaaatgac agcatttggg ctataaggaa agaaaactgg
    11101 acaatctaag gcagggatcg catcaggaac taatttcata gctatccctc gaaatgccgg
    11161 cacgatttca cctaacagta gacgtacacc agctagcaat acatacacac ccactacgaa
    11221 ttggatggct tgtaaaaagg caaacatgat atagttttga tctcctgata attcagaagc
    11281 gaatggttcg cctgcaaata aaacagtgat gatatatagc gggaccatca cgaccatgac
    11341 agacaaataa gtatcttgca gaaattcgaa atgtgtcggc aacttgatat cttcgatcga
    11401 ctttttattt tcgcctttct caccaaaaag cttagcaaca ccggcttcaa ataagtaccc
    11461 gatcgtacaa aaatgaccta aagcaatatc atttgatcca gtgattttac gaatgatcgg
    11521 ttgcgctact gccggcatag caatggcgaa acaagctccg ataaaaccac cgaccaagat
    11581 caatacgatc cctcgcagac cggcaaaata gccaaagact gtcgtcatcg ttgccatcca
    11641 taaaattgct tgaccagtta aaaaaatgta cttccatttt gtgaaacgag ccaaaataat
    11701 attgaataca aaaatggcta agaacgtcag tgcaatatct ctacctaaac ccaaatcatt
    11761 cattgcttga ccattgatag attcaataga agggatgatc ccttgcatat gaaatccttc
    11821 agtaaagatt ttcccgaaat aagttaaact tccaacgatg atgctcgaac cggcactcaa
    11881 cacttgaaat cctaggagtg tctttaacgt gccagagatg acttgtcctg ctgatttctt
    11941 ctgcagtagt agacccaaca tagcaatcaa tgcaatggtg atcgatgcct gagtcaaaat
    12001 attttcgatg ataaaattga taacgcccat gtt
//
LOCUS       DACDIH010000074        11851 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDIH010000074 DACDIH010000000
VERSION     DACDIH010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.074"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1510
                     /locus_tag="IV281_002235"
     CDS             <1..1510
                     /locus_tag="IV281_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8781208.1"
                     /translation="VDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVS
                     KEQMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSR
                     FPERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGR
                     GFVREIEATPEDKKRIESGQIEVIKKTYYTIEKVFDITQTQLKPEDYPKIFPNRVFDF
                     QLDKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVM
                     NTRNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTLEKAIP
                     YIAAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVK
                     QEHKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILK
                     GKSYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            1571..3040
                     /locus_tag="IV281_002236"
     CDS             1571..3040
                     /locus_tag="IV281_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HAP8781209.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3051..5060
                     /gene="topB"
                     /locus_tag="IV281_002237"
     CDS             3051..5060
                     /gene="topB"
                     /locus_tag="IV281_002237"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8781210.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            5406..6002
                     /locus_tag="IV281_002238"
     CDS             5406..6002
                     /locus_tag="IV281_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8781211.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            6015..6914
                     /locus_tag="IV281_002239"
     CDS             6015..6914
                     /locus_tag="IV281_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP8781212.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            6917..7048
                     /gene="ssb"
                     /locus_tag="IV281_002240"
     CDS             6917..7048
                     /gene="ssb"
                     /locus_tag="IV281_002240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8781213.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            7070..7402
                     /gene="ssb"
                     /locus_tag="IV281_002241"
     CDS             7070..7402
                     /gene="ssb"
                     /locus_tag="IV281_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8781214.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            7424..7681
                     /locus_tag="IV281_002242"
     CDS             7424..7681
                     /locus_tag="IV281_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781215.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(7840..7962)
                     /locus_tag="IV281_002243"
     CDS             complement(7840..7962)
                     /locus_tag="IV281_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP8781216.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            8152..8376
                     /locus_tag="IV281_002244"
     CDS             8152..8376
                     /locus_tag="IV281_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781217.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRNQLVIESMQIRNPDHFTRLMGI"
     gene            8824..9030
                     /locus_tag="IV281_002245"
     CDS             8824..9030
                     /locus_tag="IV281_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP8781218.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPYTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9030..9281
                     /locus_tag="IV281_002246"
     CDS             9030..9281
                     /locus_tag="IV281_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781219.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9297..9734
                     /locus_tag="IV281_002247"
     CDS             9297..9734
                     /locus_tag="IV281_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781220.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGQWFFQALLCLEAGVKPNTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYLENVNKYGACYIMKKEDNENE"
     gene            9727..10422
                     /locus_tag="IV281_002248"
     CDS             9727..10422
                     /locus_tag="IV281_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781221.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEELNSKDATVDDYYLVFTKFLTCGTE
                     SIVLMGMDYELAVPEVFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRK
                     RMPYQRFERKKGQ"
     gene            10803..10982
                     /locus_tag="IV281_002249"
                     /pseudo
     CDS             10803..10982
                     /locus_tag="IV281_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11044..11228
                     /locus_tag="IV281_002250"
                     /pseudo
     CDS             11044..11228
                     /locus_tag="IV281_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, HicA family"
     gene            11262..11693
                     /locus_tag="IV281_002251"
     CDS             11262..11693
                     /locus_tag="IV281_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HicB family protein"
                     /protein_id="HAP8781222.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
BASE COUNT         4317 a         2002 c         2487 g         3045 t
ORIGIN      
        1 agtcgatcag atccgaatgg atctagggga tggcgtcaac agtaatcaac ccatctattt
       61 agagttagca aaattgtgcg aacaagtacc tgagaaggaa caaaaacaat cggtcaaaaa
      121 tgaacctgtt tcgaaagaac aaatgcagtc gaaagaaaca aaaacaaaac aaaagggcta
      181 tcaaaaacga acggcagaag agattaaaca agaagttaaa tccctttcgc aacaggcact
      241 ggatgcggtg aaaaagcata ctcattctcc tgaagacgtc aaagaacttt tgaactttat
      301 gtctcgtttt ccagaacgat cctttagaaa tcaattattg attgaggaac aatttccagg
      361 tgcaacggct tgtttaggtc gtgccgcgtt gaaaaaagaa ggaatctata tcaaaaaagg
      421 ggaacaaggg aacaagatct ttgtcagaaa aacggtcaaa ggattttatg agaaaggacg
      481 tggctttgtt cgagaaatag aagctacacc agaagataaa aaacgaatcg aaagtggtca
      541 aatcgaagtg atcaaaaaaa cttactacac gattgaaaaa gtgtttgata tcacacaaac
      601 acagttaaaa cctgaagact atccgaaaat ctttccgaac cgtgtctttg attttcaatt
      661 ggataaaaaa ggacagtcag aacttcaagc tgggattcaa gcggtagcca acaatattgg
      721 aatacaaatt cgagatatga cagaaagtga agtctatcaa agagaattag gacaagcacg
      781 tggggcgtat gttcgtaacg aatttactgg cgaagaagaa atcgtcatga atactcgaaa
      841 tactcctacg cagcatttag ctacaagtat tcatgaatta gcccatgcaa gaatgcacaa
      901 attttcagaa ttagataccg caactaaaga attgcaggca gaaatgactt cttatattgt
      961 gtgcaaacat tttggaatgg acacgttgga gaaagctatt ccttatattg cagcatggac
     1021 gaagaatggt cagacgttgg atgataagga agctgcggaa cgtgggaaaa tcatgaatga
     1081 tgtcagccgt gtcgcaaatg aattcattca aaccatttct aatgaaatca atcaatacag
     1141 agaaagagaa ccagagatcc aaccagcaga agtgaagcag gagcataaaa atactgtaga
     1201 aacggcagaa caaaagaata ttgttcctgt tggttcactt tggatcgaca agaaagacgg
     1261 gaaagtaatg gaagaagata cgggtcgaac gttacatttg aaggatgcgg cgtttattga
     1321 agatccacag cataatcaag taattttaaa aggaaaaagt tatggaaaag agatccaaac
     1381 ggcgttacct tcagaagaat ttgcgctagg aaattcattc cgattttcac ttgagacagt
     1441 gtcagaagtg gcaaaacgaa aaaatgaaat ctattatgaa caacaattgt cacaaaaaga
     1501 acgtgtataa aacaagtaaa aaagactatc tgaaaaacgg atagtctttt ttcttttggg
     1561 aggttttaaa atgaccgaga aaaaaatgag tcttattgat cgatgtaaac agatcgatat
     1621 agtggatttt gctcgaaaca atggaatggc tgtagtcaat aaaggacgag actatcgctt
     1681 agaagaccat gattcatttg tttttgatcg aagaaaacaa cgtttttatt ggaacagtca
     1741 aaatattagt ggcgacatta ttgaattagc taaacttttt ttcatagaca aagagatcca
     1801 agaccctaag caacaattta aagcagccac tgattttatt ctaaagaatg aagacaaaac
     1861 agaacgagtg gaaaatcttc attttgagac agaaaagtac aaagatcacc ccgttgatta
     1921 tcaaccactc acagaaaaag gaaggaatta tctgaaggaa gaacgaaaac taccagagtg
     1981 gctgattgat tatgcagaaa aagaagggtt gattgctgaa ctaaagccga aacatgaacg
     2041 gcaaaatttt ttagttggag acgaccgttt agatcatgcg gttgcttttt tatggaaaga
     2101 tccacagaca ggagagacag taggggcaag ttatcaagga acgatcgtcg attttaatcg
     2161 tttcggtaaa cggggaacgt ataaacacat tgacaagaat cctacgccaa atcacggatt
     2221 taatttaaaa ataggcgatc cgaagcattt aaaattcttt gaaagtagta ttgatctgtt
     2281 aagttacgct gcattgaatc gggagaaact acaagatgcc tggttagttt cgatggacgg
     2341 gttaaaacat catgtaatca gtcattacgt tgaagagtcg atttctgaat taagacgaaa
     2401 acaaacattt cctcaatcga ttgaaatatg tgtggacaat gatcgtgcag gacatatttt
     2461 ttatgaaaaa gaacagatga agggaatcgt tgatcctttt accaataaga aaatccgttg
     2521 tgagcgagga attccaaatg attggcaagt acctaaagaa tacaaagcta cttatgaagc
     2581 agtcgcaaaa gaaatgagtg ttgaacctga agcaattatg gccatacaca aaactgaaac
     2641 taatttacag ctgacaaatc aattggtgtc tgctcatgat gttcaatcta cgtttggaaa
     2701 gatgttggcg aagggcgaac cagtagaaac aattgatttg aaagaagcat gtaccacggt
     2761 cgcgaaggaa ctgaaagtat gtgaacgcgc agatggaaca tacaactttg atcgttttta
     2821 tagtagaaaa gccaacataa aagacgttaa tgcaggaatc cttctttcct ataaagcaga
     2881 acaatactac aaaggctata aaaagcatga acatgaattt gtacctgaag tgaaaaagga
     2941 ttggaatgat caattgaagc atgagatcca gcaacaagaa atcagaaagc aaaaacgtgc
     3001 gatgttgttt caacaaggca gacaacaaga aagggagtga agtcgtcact atgaaaaccg
     3061 tcattttagc agaaaaaccg tcacaagcga aggcttatgc cgatagcttt tctaaagcta
     3121 cccgaaaaga tgggtatttt gagatccaag atcgattgtt tacaggagaa actgtgatca
     3181 cgtatggttt tggtcattta gtcgagttag attcgcccga tatgtacgat gaaaaatgga
     3241 aacaatgggc attagaacat ttgcctattt tccccaatca ataccactat catgttccta
     3301 aagataagaa aaagcagttt agtgtcgtca aacgacagct gcagtcagct gatacgattg
     3361 tgatcgcgac tgatagcgat cgtgaagggg agctgattgc ctggtcaatc attcaacaag
     3421 caggcgccaa tcaaggaaaa acctttaaaa gactttggat caactcctta gaaaaagaag
     3481 ccatttatca gggattccaa cagttacgag acgcagaaga gacctatcca aaatttgaag
     3541 aagtacaagc acgtcagatc gccgattggt taattgggat gaacggtagc ccactgtata
     3601 gtttactctt gcagcaaaaa ggcataccag gaagtttttc tttaggtaga gtccaaaccc
     3661 caacactcta tatgatttat caattgcagg aaaagattag aaacttccaa aaagaaccat
     3721 attttgaagg aaaagcccaa gtaatcgctc aaaatggcgc atttgatgcg aagcttgatc
     3781 caaatgaaac ccaagcgaca caagaagcat ttgaagccta tttaaaagaa aaaggtgttc
     3841 aattaggaaa acagccaggt acgatccttc aagtcgaaac agagaaaaaa agtgcggcta
     3901 gcccacgtct tttttcttta tcgagccttc agtctaaaat gaatcaactc atgaaagcta
     3961 gtgccaaaga tactttggaa gccatgcaag ggttgtatga aggaaaatat ctaagctatc
     4021 cgcgaacgga taccccttat attacggaag gggaatatgc gtatctgttg gatcacttag
     4081 acgaatacaa gcatttttta aaagccgaag cgattccgac tcctattcat acaccgaata
     4141 gtcggtatgt gaataataag aaagtccagg aacattacgc gatcattcct acaaaaacgg
     4201 ttatgacagc agcggctttt gaacaactaa gtccactgca gcaggctatt tatgaacaag
     4261 tgttaaaaac aacggtggcc atgtttgcgg aaaagtatac ctatgaagaa acaacgatct
     4321 tgacgcaagt tcagcagctt cagttaaagg caatcgaaaa ggtgccgcta gatctaggtt
     4381 ggaagaagtt atttggaaaa gagagcgacg gaaaagagaa ggaagaagag ccgctacttc
     4441 ctaaagtgac aaaaggagaa atggtgacgg tggatctaca agttcttgaa aaagaaacca
     4501 agccaccaca accttatacc gaaggtacgt tgatcactgc aatgaaaaca gctgggaaaa
     4561 ccgtggatag tgaagaagcg caatccatat tgaaagaagt ggaaggaatc ggtacagaag
     4621 cgacacgggc gaatattatt gaaaccttga aacaaaagga atatatcaag gtagagaaaa
     4681 ataagctcgt ggtgacgaat aaagggattt tgctttgcca agccgtggaa aaagagccgc
     4741 tgcttacgag cgccgaaatg accgccaaat gggaaagcta cttgttaaag atcggtgaac
     4801 gaaaaggaac acaaaccact tttctagcca atatccaaaa atttgtctct cacttattgg
     4861 aagtcgtacc agggcaaatc caatccactg attttggatc gacgttacaa gaagtgaaag
     4921 cagccagtga aaagcaagaa gccgcgcgtc atttaggaat ttgtccaaaa tgccaggaac
     4981 aagaagtctt actctatcaa aaggtggcag cttgtacgtc tgaagcctgt gattttaaaa
     5041 ggtcagaagg gttcatttga agcaacgatt gttttaaaag aagacttcac tacaggcttt
     5101 gaattttctg aaaagaaaaa aacgaacttc aagaaaagaa cacgccgcac tgcaaaataa
     5161 tggagggtta atcttatgcg taataccaca cacaaacaac aagaaatcga aaatcaaaaa
     5221 gccaaaattc gttcagaaca ggaaaaaatg cgtgccagca aacgaagact gcagcaattg
     5281 aagaaccagc agaggattca aaaagaaatc gaattagatc gataaaatct tcctaactat
     5341 tgtatacgta taaacttatg ttatactgtt tttataagtt tatacgtata caaaggaggg
     5401 agaaaatgac aaaagcaatg gtatacaagc tgctagaaga atttaatgga actttggctt
     5461 tgaaggatgc caaaaaagcg ggcatttctc ctgtaacgat caaacgtatg gtagatcgag
     5521 gggagctgga tcgagagtat ccaggttttt tcaccttacc tggtcaattt cctgatgaat
     5581 tgtttatggc gcaaaaaaaa tatgaacgcg gcattatttc acatattacc gcgttagatt
     5641 tgtatgattt aacagatatg atccctagac agatcgatct gactgtacct tacggttatc
     5701 acgtttctga aaaaggattg aaggaattcg cagttgagct acactataca aaagcggaat
     5761 ggtatgaatt aggtaagatt gaaataaaaa gtcgctatgg gaatcctatt atagcttatg
     5821 atccagaaag aactttatgt gacatttgga atccctggta taccgttgag gatgaaatca
     5881 aagtaaaagc aatcaaaaac tatatggagt cggacagaaa aaatcttaga aaattgaatg
     5941 agtatcgacg aattttacca acagacaaaa cgatgcgttc ttatattatg gctttaaatt
     6001 aaaggagaag atacatgaca cctacgcagt taaaagcaaa agtgaagaat atttctagag
     6061 aaaaagaagt tgatccgcag cttgttttaa gacattttat gatggaaaaa ttcttggaaa
     6121 aaatctcaga gtctccttat cgtgaaaatt ttgtgttaaa aggcggcttc ttgattggct
     6181 caaagtatgg cattgaaaat cgcacaacca aagatattga tacgacttta cgggaaatga
     6241 aagtaaccaa agaaacatta acaacggttc taaacgaaat tttttcaacg ccgacgaaag
     6301 aaggcattca atttgagatt caaggaatga aagaaacgag agaagccgat tactatccag
     6361 gatttagctt acgtgtgctg gcacatttgg aaaacatgcg gccagatttt aaggtagatg
     6421 tgactacagg tgactcgatc tatcctgcaa cgatcactca cagtcataaa ttgatgtttg
     6481 aggatcgaac gattcctttg gaatcctatc ctacagaaca aattattgct gaaaaattaa
     6541 gtgcaacctt tgatttttta acagataatt cgagaggaaa agatttttat gatctctata
     6601 ctattcctaa aatggagaag atcgatgaga agaccttgaa agatagtgtg agaaatacat
     6661 ttacacgtcg aggaaaaact gatccgttaa agatatacta tgaaaaggat atggatgtac
     6721 tgaaaaatag tcacgagcta aaagatacct ggaagaaata tcaaaaaagc aatccttatg
     6781 ctgcgtcgat cacgtacgat gagacaatgg attcgattag taatttgatg gaaaaagtga
     6841 ttcacgtcga agaaaaagag cgaaaactag cttatcaaag acaccaacag atgaatagag
     6901 aaatggagag ataaagatga ttaataatgt gacattagta ggacgcttaa ccaaagatcc
     6961 tgatttacgt tataccgcaa gtggtactgc agtagcgact tttaccctag ccgtcaaccg
     7021 aaatttcacg aatcaaaacg gcaattgaga agccgatttt attaactgcg tgatttggcg
     7081 caaaccagca gaaacaatgg cgacattagc taaaaaaggt agcttgattg gtgtagttgg
     7141 gcggattcaa acgcggactt acgacaacca gcaaggacaa cgtgtatatg tgactgaagt
     7201 tgtagcagat aactttcaac tattagaaag taaagcagcg acagagagcc gtgcgcacgc
     7261 tgatcaatcg tccaccagtc caagcaccgc cacctttgaa caacggaata cagcgacgcc
     7321 taacaacaat ggattgaacg cttcacagaa tccatttggt ggacaatcaa tcgatattag
     7381 cgatgatgat ctaccgtttt agtttgagaa agaaggagta agcatggcaa aaaaaattag
     7441 tctgacgatg gatctattga tacccgtaat tgcatttata gacgggattt ctttgggcgt
     7501 cactgaaaat cctttggtaa atagaattgc gaaaggatca ctatttcttg tattcgtgta
     7561 tcttgtctgt tcttatcttt taaaaagagc aggaaaggat aattcctttt ggcgattgac
     7621 taaagtcaca gtagcagcga cagctgttat acttacggct attctaccgc tttatatgta
     7681 gagttaacgg gaaatcgttg aacttcttgc gaaatattgc tataatgaac atgaaaaaga
     7741 ggagttgcgc taacaacccc tcattaacag caccgattaa agaacggtgg cagaatatta
     7801 gttatttgga aataaccgct gacctgctaa agtagacggt tatttctttt tgtcattttt
     7861 aagcatattt acaatcaaac taatcaaagc aatggtaaac ataccaaaac caagaatggt
     7921 ttgaattgtc tcatatgcgg acaaaaggct tactcctttc atagatttta gcacttacat
     7981 tcataagcac caccaccttt ccagaagtcg ccaccgtctt caaactttac tgtcttgact
     8041 attatagcat atctaaaatc gaataaaaat tctcaatttt agctgttttt gctataattt
     8101 tagcgaggtg atcgtgatgg aacaatatat tggtttactc gataaattag ggtgatcaag
     8161 acacagccat tgcttgtgcg atttacatta cgcacgagtc atgaaccaat caattgtgta
     8221 gtggccaata tagaaatagc ggataaattg ctgattttgg aagaaggcaa gtatactatt
     8281 tcagtgacag gtcacttcaa taaacgaaat caactagtca ttgaatcaat gcaaattaga
     8341 aaccctgatc attttacgag attaatgggc atataaataa aagaagagct gcagtcctaa
     8401 atgaggattg cagctctttt tgttttacat agaaacaaga aaaatgaaaa ggccttttta
     8461 ggaaggcgca agcgccttct caattgaaaa acctctctct cactccaacc gccaccgccg
     8521 ttcgagaaga aaggaagtga acgttgcacg ttcactaaga taaggggtct tgcaaagcaa
     8581 gacaaaatag aaaaaacttc ctctcaccaa aaggttcgtt cctgttcgct atctctttat
     8641 tgatttgcca gaccggcaac aagttgcctt ttatcaccaa aaagcgacaa ggacaagggt
     8701 aaatggcacg ctgcacgccc ttgtccttgt cgcttttagt tgattcattc tggccaacaa
     8761 atcaataaag aaagaactaa cgaaaggaag taaaaccttt cctaagagga ggcagaatca
     8821 atcatgcgaa agaaatttga ttattggggt gttcccttca gcgaattatt cccaaactat
     8881 catgcaccat atactgtcga atgcgattgt ggagaaagag cgaaatgtat caaaagctat
     8941 tgtttgtatc aatgtccaac atgcgggaaa aaatatacct tgtcttatgg cgattatatt
     9001 ttgattgatg agaaaggaaa gaaacgctga tgaaaacaca acacaaatta ttgactgtaa
     9061 aagaagtcgt tcaaattttg gtggacaaag aagtcatttc tgaaaaagta gcagaagctt
     9121 tacttattcc gcggtactgg acgatagaaa cccctttagt agaggatgca ccgactatgc
     9181 tatttgaaga cctagtgaag attgcaggat atgaactgca ttatcaagaa aacatggaaa
     9241 cttatgaaaa cgaatattgg ttaaccaaat tagagaacta aaaaggagaa aaaaatatgg
     9301 ctcaaaatta ctatgacgaa tttgtcaaat tacctttgga taaaatggca caaaaaatgg
     9361 aggatatgac attcctatac aatgagacac gtgttccaaa aaaacattat aaggaaagat
     9421 tgtcggtagc tgtggaagaa atgattgaat cgggtgtcga aatgaatctc attgcgacct
     9481 actatcgaac gcttgaagaa ctgaaaaagc aaaatggaca atggtttttc caagccttac
     9541 tatgcttgga agcaggcgtc aagccaaaca ctatcaaacc ttctgaatac caagctttag
     9601 aactaacgta tgggaaattt gtcgaaacga aaaaagccaa gactgtttca agtgaatggt
     9661 tggactactt agaaaatgtc aacaagtatg gcgcatgtta catcatgaaa aaggaggaca
     9721 acgaaaatga ataaggtgat ttatcaacta acaacggaag atgtgtcaca ctttttaaca
     9781 aacaaagaac atttgaatga agtaatcaga cggattcaaa aagactttag ttgtatggaa
     9841 gaaatgaagt actttattca aagagtcgaa gaacaattgt atttggaaag tgaaacgagt
     9901 caaacgaagg ttacagtcag caataattat ttagacgtgt ataaacgtcg tcctgacaca
     9961 ttaaaaaaag gagcggagct tctacgacta gaaacagaaa aatatatcat gaatgtttac
    10021 ctagaaagga gtgaactggt cgaaaacttt gtcagctaca cgatttgcag gaaacactat
    10081 tactcagaat cagaatatta ttcaattttt gagagcatgg aattaatgga agagctaaac
    10141 agcaaagatg cgacggtaga cgattattac cttgtattta ctaaattttt gacgtgtggg
    10201 acggaatcta tcgtgttaat gggaatggat tatgaactcg ctgtacccga agtatttttg
    10261 agggaatatg tagaagaagt agataaccca ggcgacgact ggtacacgac tttaactgat
    10321 tttttagata cctacactta cgacgataca gaagaaatcc aaaatgaact tgaccgtaaa
    10381 cgaatgccat atcaacgatt tgaaaggaaa aaaggtcaat aaccatttat accgaaaagt
    10441 gagaatgtgt gaaataaaaa caaaaaacct tgaaattaag ggaatggttc ttatttcgca
    10501 ccaatcgctc caaccgcccc cgccgcttag gtggtgcgaa ataagcgagc aaagcccgaa
    10561 aacctaagag aaagaaggag atttatatga acaaaattat tcccttatta ttagtcctat
    10621 ttgttggaga aatacttatg cgacttagtg cagctgtaca tttaaacctc gcagtatttt
    10681 tggtaatact aatgttttgt ttgatctttt ggcgatttgt attagccctc gtaagtctac
    10741 tattcaagat gattgcagcc atatttttca taggattatt tcgatgaaag gagagagtac
    10801 caatgccaat tgcaatagga aataaacggt tacccgtgac gttggatgaa aagagacaaa
    10861 aagaattgca gtaaccaaag cagaagtacg gcaaaagtga atccaggatt atgtgtattg
    10921 cgttagattt attgattgcg caagaaaaag caggatttga cataccagca ctcaaaaagt
    10981 gatcgcactt tgcgcacgtg ttaatacgtg ttataattat tttgtaagga ggtatagtgc
    11041 acaatgccac ttacgggaaa agaaatgctt aagttattga aaaaaatggc tgggttgagc
    11101 gaaggcaaga gggatttcat catcatcttt ataaggatgg tgttagaatc actgttcctg
    11161 ttcatgccaa tcaagatctt ggccgaggac ttgagcgaaa aattttaaag gatgcgggat
    11221 tgaaataatc tcgcattcga ttaaagaaag gattgactga aatgttaagt gagcctttag
    11281 caaaaaccta tcctgctatt tttagcccag aagaaggtgg tgggtacttt attgaatttc
    11341 ctgatgttca aggtgcttac acaggaatta acgaagacga tatatcttat ggaattgcta
    11401 tggcacaaga ggtgttaggt atggttttag cagattatat tgagcatgaa gatctacttc
    11461 cagaaccaac accaatcaat aagatttctg ttgaagatga ttcttttact actttaattc
    11521 gtgtagatgt tgccaaatat ttgaaagaca cagaacttgt caaaaaaacc ttaacaattc
    11581 ctcaatgggc ggataagcta ggcaaaaggg ctggcataaa tttttcagtc cttctaacag
    11641 aatcaattgc agataaagca gataacattc ttcgttctgg aagaaataat tagaactcaa
    11701 aagggtcaga aacttaaaaa tctttggctt ctttttttac tacaaagatg gcttctttaa
    11761 attatttgcc tgattgattt aaaaaaattg cgcaaagact acgagctaat taagttgctc
    11821 gtggtctttt tttatgaaaa ctttttagat c
//
LOCUS       DACDIH010000075        11799 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDIH010000075 DACDIH010000000
VERSION     DACDIH010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.075"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(39..779)
                     /gene="truA"
                     /locus_tag="IV281_002252"
     CDS             complement(39..779)
                     /gene="truA"
                     /locus_tag="IV281_002252"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8781223.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(970..1605)
                     /locus_tag="IV281_002253"
     CDS             complement(970..1605)
                     /locus_tag="IV281_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP8781224.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(1752..2993)
                     /gene="sstT"
                     /locus_tag="IV281_002254"
     CDS             complement(1752..2993)
                     /gene="sstT"
                     /locus_tag="IV281_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP8781225.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            3191..4189
                     /locus_tag="IV281_002255"
     CDS             3191..4189
                     /locus_tag="IV281_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781226.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            4202..6220
                     /locus_tag="IV281_002256"
     CDS             4202..6220
                     /locus_tag="IV281_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP8781227.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(6280..6996)
                     /locus_tag="IV281_002257"
     CDS             complement(6280..6996)
                     /locus_tag="IV281_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP8781228.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(7223..7765)
                     /locus_tag="IV281_002258"
     CDS             complement(7223..7765)
                     /locus_tag="IV281_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8781229.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(7770..7997)
                     /locus_tag="IV281_002259"
     CDS             complement(7770..7997)
                     /locus_tag="IV281_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP8781230.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(8076..9956)
                     /locus_tag="IV281_002260"
     CDS             complement(8076..9956)
                     /locus_tag="IV281_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8781231.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(10145..10759)
                     /locus_tag="IV281_002261"
     CDS             complement(10145..10759)
                     /locus_tag="IV281_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8781232.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            10901..11461
                     /locus_tag="IV281_002262"
     CDS             10901..11461
                     /locus_tag="IV281_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8781233.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT         3685 a         2358 c         1901 g         3855 t
ORIGIN      
        1 actgtgaaac atgcgtttca tccattatca aagtattttt atcgataata aacttcatcc
       61 aaaaacagac cttgtgcagg gacggtttct cctgcatgtt gccgcacttt gctttcaaat
      121 atttcatcga ttgtttctat cggcatggca cctgtgccga tttctagtaa tgtccccatg
      181 ataatccgca ccattttata taagaatccg tctccgacaa aagtaaaatg aagcatttct
      241 ccctttcgtt cgatagataa ttcatcaatc gtacgcactg tggatttttt tgattttttc
      301 aatgcagaaa aaccaatgaa atcatgtgta cccactaatt tttcgcaagc tgcattcagc
      361 ttgtccatat ccaactcttg agggaaataa aagctgtggt ttcgctcaaa tgctgaagga
      421 atcacgttgt tccaaacata atagctgtat tttttgccca ccgcattata tcttgaatgg
      481 aagcgctcag gcatttcttc gattcttttg atgacaatat cccgcggaag gtaacgattc
      541 ataaagtcga tcatttctgc cagtccaatt tctgtgtttg ttttaaaatt tgctacttgt
      601 cctcgcgcat gggtgccagc atctgttctt cctgatccaa taatttctat ctcttcattt
      661 gtcatctgat gcaatacttt ttcgattttt ccttgaatgg ttttatcgtc atctccaagt
      721 ctttgccatc ctgaatatct ttttccgtca tattcgattg tcaacttgat gtttctcata
      781 ttttagtggc tcctctgtct tccatttggt cttctctcta cctttttaag attgtagcac
      841 aaaatatagc tccttgcttc tctaagacag aagctataca agaaaactgt ggaaatatat
      901 atttcagaaa aacaaaaaac agccaccgga tgaagtatcc gctgactgtt caatttgacg
      961 gctcaagttt tactgtttga gatgcaagat actcaaccag gcaatgggca cataattaaa
     1021 gaaaaaactg attctttggc aaaaacctat tttattcagt aatggccaag aatttataga
     1081 aggaattctt gccagtccat aaatcgccgc aaaaagcaaa ctgatcacag acagtccaag
     1141 gtataagtgg cttacgtcca tttgcccatt ttttcgatag atcaagaaaa gaagaactgg
     1201 aaacaataaa aaaccactgt aacctaaacc tgatccgata ttatggaccc atgtcgagac
     1261 attccacgaa gattcatggg tattgatact gaacaaacct gtaaagatac aatcacctat
     1321 accatagaac gcgatggctg ctgtaactag ggtagctaac acacgagatg tactaacaaa
     1381 tgcagcatac aatgcaggca atgacaggat gaaaaagatc cctgaaacca cagaccaaat
     1441 aagaaacgct tttcttacag gactggaaac ctctccaaat acactgatca cgctcttcat
     1501 ttgatctaat tcaggataat acaatcctaa tacgtacgga gtcaaaaaat cactcagcac
     1561 gcctaataat aaaaagaaaa aaccaaattt acgtaaaaaa tccatgatac gcctccaata
     1621 acaattacct atgcttttaa ttgttattat aaacgacttt atcttgattt aacagaaacc
     1681 tctatttaca cgaacatcta tataactcat taaaaactcg aatcatttta aaaatactaa
     1741 atatctaacg ttcaacgttc acttaatcca tctaaacaat ctttcaattt gattttttcc
     1801 ccacttcttt tacgcgcgcc tagctctcct attgcagtaa acaacagatc acttgacgaa
     1861 tttaaggcag tttccattga atcctggata acaccgataa taaatccaat accgacgatt
     1921 tgcatcgcga catcattaga gataccaaac aaactacatg ctaatggaat caataacagt
     1981 gaaccgcccg caacacctga agctccacaa gcagctaaag cagacaaaat acataaaagg
     2041 actttcaaaa caaaaggtgc ttcaatccct aaagaatgta cagcagtcaa cgtcatcaag
     2101 gaaatggtaa tcgcggcacc gcccatattg atcgtagcac ctaacgggat cgatatggaa
     2161 taagactctt ttgtcaattt caatctttca gccaacatca tgtttatcgg gatatttgcg
     2221 gcagaacttc gggtaaagaa agcgggaagt gcgctttctt tcaaacaaaa gaaaatcaat
     2281 ggatagggat tttctttaat aaacaaaaac gtgaggaatg ggtacacaac taatgcgaca
     2341 aacagcatcg taccgatcaa aacaagtaaa agctgcagat actctgccaa tccagcgatt
     2401 ccagtcgtag caattgattg ataaaccaat cctaaaatcc ctatcggagc aaatgcaata
     2461 atcacttgta cgacttgtga caacgctata gatatctgat ccaccaactc ttttgtcgat
     2521 tctgaacgtg ctcgaaaagc tagtcccaac aatacagccc agaataagac gccaaggtaa
     2581 ttcgcatcaa taagagattg aacaggattt tgtgtgacat tcgtcaacat agtagataaa
     2641 acactttcca aactttgcgg tgctttttca gatactgttt cttgtaaagt aataggaaca
     2701 gtaaaaattg aacttacaat aactgctaca agtgcagaaa gcaatgttcc cactaaatat
     2761 aagatcaaaa taggcttcac gaatgtctta ttcccaattt tatgcttggc aattgacgcc
     2821 atcgtcaaga aaaaaaccag aataggtgcg attgccttca agcaactgac aaacaacgtt
     2881 cccaatattg aaataaatgt ccatgtgggg aaaaagatac caaacaatat tccgactaaa
     2941 ataccaacaa caatacgttg aactaacgac atatccaaaa acttctctaa caaatgacca
     3001 ccctttcaca attttaacaa tcattgtatc attttatgca aagaaactaa aggtataatt
     3061 ttctaaaaat tctaacaata caaacaacca tttcttgcta attttgtact ttttctgtct
     3121 acacgttgtt ttttgagata ttctaagtta ggatgttatt aatgggcaac ttatcaggaa
     3181 acgttttctt atgtttttgt ccaatttttt tgtgaggtgt ttattcttga aaaaaataaa
     3241 gaaggagagt tatggcttcc tgacaatctt tgtcgcttgt ataatgatgg ttttacagcg
     3301 tatattgatt tcagcagtca ctattcctac atatgcaaca aaccctggtc gattctacat
     3361 ctatacattt ttacaaactt ccgttgttat tggtgcaaat ttgatcccac tttacatggg
     3421 ttaccaatat caaaaaatcg cccgtttaaa agttttacat tatttaagtc gttttgcctt
     3481 tatattccta atagccacac tagtttgcaa tatctttttt tacgtacaag ctggcagctt
     3541 gaatattcgt gattattggc ttatcctaac acctatcagt cagaattact ttacttatgc
     3601 tgtttcttgt actttgcttt tttgtagtat tccttataca gttggatttt taaataaatt
     3661 atcgaaaaca acaattaaat atctactatt atttttaaca attggattag ttggctgctc
     3721 tactctattt aataaagacc cgtttgcatt acagaatgga cagagtattt tatggatttt
     3781 ttatttattt ttaatgggtt atggtttgaa agaatggaac tggaaagaaa aggtccgcta
     3841 taagcttcct caagtattta tcagcttgat tgttttattc ggtttgatta ttttgatgac
     3901 acaaatttca ctcatcatca gaggaaatac agatacagcg ctgaggtttt ctaaaccata
     3961 ttctctcttc gcaatgtatt atactttctc ttcgttttta tttttagaaa tgctatctga
     4021 taaattaggt ataaaaggaa gaggaaattt tcttgctgta tttttgattg tcactcaagt
     4081 tatatgtaat ttccctttag tttcttatcg cgtatctaca tttatgaaac gtgaatttcc
     4141 aaactcaggg aaggcatggc tgataaatat cgggcagttt tttatttgat atttattagc
     4201 agtgtccctc ctagttatct ttttatttta tatacaaaaa atccgtttca ttcatcgttt
     4261 catcgaatac ttcgcttttg attcgattga acaattaact cagcgttgga aaaaaagaat
     4321 tcattggctt tttgttcata aaagttattt tttagttgca gcattttttt actgctttac
     4381 cttcgtgcag attttcgttt tagaaccaaa agaaggttgg aaagaaacta tccaggttgc
     4441 cttaaaaata tttactcagc gacaagcacc catgatcctg acaatcatca ttattatggg
     4501 atttttcttc ttactattgt tgatttccaa tcgtttttgg tacgccttga ctgctacctt
     4561 gatcattaac ttactgctca ctatttcgac agtaatcaaa atggaaatgc gggaagagcc
     4621 tgtctttcct tcagatttaa aaatgctgac cggattgtca gaactattat cgatggttag
     4681 cccagtctta ttaatcgtgg gcggattgat ccttctccta ttattgatta ctagtattat
     4741 tgtacaacgt agactacaaa aacagtattc cttgaagatt cattggaaac gtcgatttat
     4801 cagtatcgct gtattattag gatgtttttc gggtgtcttt ttcattaatc acaaaaattc
     4861 gccttctttc ttgctgttta atttatttaa agtaaacaag actttcttta accagcaagg
     4921 cgccgtaaaa gataacggac cagtcattca attcctaaac aatatcgaca ttaaaatcat
     4981 ggaagaacct gctggctatt cgaaagaaaa aattaatgga ataatgaaaa agtacaacac
     5041 aaaggcagat gaaatcaacc aaacaagaag tgactggtta aacaatcaga cgatcgttct
     5101 caatttaagt gaaagcttca gtgatcctgc acgtgtccct aatctgacag tacctacaga
     5161 ccctattcct tacatcaaaa gcattataac agaaggaaca ggaggattga tgttatccgt
     5221 cggttatggt ggcggaacag ctaacatgga atgggaagga ctgacaggct tgagtatcag
     5281 taacctgtct gcttcgttag ttacacctta tacccagcta gtggagcgtc agaaaataag
     5341 tccaaatatt accgatctat tcgatgaaaa aatagccatc catcctttta cagctgctct
     5401 ttatcgcaga aaacaagtgt ttgataaatt tggttttgac aggttctatt atttagacag
     5461 tccggataaa ttaacttata cggataaaat agaacaaagc ccacgcatat cggatgattc
     5521 agcatacaaa gaaaccttga aagctttaca aagcaatacg gaaacgtcac aattcatcca
     5581 attatccact atgcaaaacc atatgcctta tgatgattac tatagtgaat tgaactacac
     5641 tgctgaagga actgctgtca ttcctaatag aagaaaagaa ttacaaactt atatgcaagg
     5701 ccttcactat accgatacag cagtaaaaaa attcatccaa gaaatcgaca aaatcgataa
     5761 accgattacc tttgtttttt atggtgacca tttacccgca atctatagtg ggaatgattc
     5821 gaaaaaatat ggattagaac agcaccagac agattatttc atttataaca acaagtatag
     5881 tcgtgaacaa gcagctacgt taaataaaga agttgttgct cctaataact ttggtgctat
     5941 ggctcttgag catgcgaata tcaaagtaac tccttactat gcgttgttga cagaagtatc
     6001 taatcatcta cctgcattaa caattgatcc tacagaaagc ttgtctaatc gttttaatgg
     6061 aaaacaggtc tttgttacag aggatagtca aattatccaa gaaaaaaatt taactgagaa
     6121 acaaaaagaa attctagaag attatcgtct gatacaatat gatcttgttg caggtaacca
     6181 gtacagtgca acatgggcga cacaaaaaat aaaagactaa caaataaact gagcaagggg
     6241 ttgtgataaa agacatgtca caaccccttg tttatctcat taatttgctt gtccataaac
     6301 gatttgatta aagccatttt tcaattcatt attcgctgtc tcgttgaagt aaagataatt
     6361 atcgggattc ccctgatttt gttgtgtcac caatactttt ggctgatttt gctgaaaccc
     6421 gaatagatag acgtgctttt tcagatatgg ttctgtctct gcatcggaat agacggctgt
     6481 gatttgatag tctgcttgac ctgtcccact ttctgaccat tctgcagaaa caggcactcc
     6541 accaatagcc atcttccact ccccgttcaa aatagcttga gggactttaa gaccatataa
     6601 gtccacactc atttgattcg tataactttt gtattgttgt cccattgttt ggccccattt
     6661 ggtcataaat gcctctaatt gtgcggattt gtctgcattc cacagtgttt gttcaacttg
     6721 ttcgctgctt tctgaagagg aagatgaagc ctctacagaa gaacttgaag tagatgtttg
     6781 agttgtttca gtcgttttgg aagactctga cgaagtagag gtatctgctg tttctgtact
     6841 tgctgcagtt gacttgctct ctgtgctagt tgttgtagta gttgcaactg ttgctttcgc
     6901 tgtttctttt gttgattctt ttttcgattc attcccgttg gtacaaccag ctaacaaaaa
     6961 agctagacca attgttacta aaatcctttt tttcaattaa aaaactcctt tcgattactc
     7021 ccacttcttt attctatcaa ataagcaatg tgattttatt gatttttcgt attttctcat
     7081 caatctttca aatcttctaa aaactgagaa gcacccgctg atgttgtctg taatacatca
     7141 gcgggtgctt cttttatttt aatcttcgtg attttccaat ttacctcacg ctttatattt
     7201 gtatttgaag aaacatagac ttttagtcat catcgtcttc ctcatccaaa tcttgtcgga
     7261 cagtttttcc ttggagtctt tgtacttggt agatgatttc tttattgaaa cttaaaagtt
     7321 caacaagcaa ttgttgcaag ccatattttt cttctttttc agctaatttg attgcccgtg
     7381 tgataaagac attttggaaa tcaaaatctt ttgcaaagtt ttccaacatt gttgaggctg
     7441 gtaaatattt gttttgacca tgttcgtaaa tttttccata acgcatgact tcttctaaag
     7501 tagacggaac gatttcattt tcaatcacta actcatgaga gattttattg aaccatctcg
     7561 cttcttgatc agcatattct gaaaaaaggg tacttaattc ttgcgaagat gtacccaaaa
     7621 catacaattg tgcttggatc aatttcattt cttctaattt aaaatttgct aatacatgcc
     7681 ccaccatggc gccagcagtt ggtttatgat ggtcgatatc tgcttgtttt gtttcttgaa
     7741 caaatttttc ttccactata ttcattgatt tacgcctctt ttacttttgc tgattggact
     7801 tcataaccta atttttccac aactgcagct aattgttcag ggtggacttc atctggatca
     7861 aagtttgctt tgattttgct cgcattaaac aaaactttcg tattgcttac cccaggctgt
     7921 tgagaaaccg catgttcgat tttttgcata cagcttggac aagctagtgt atctaattgc
     7981 ataataattt ttttcatcgt agttcttcct tcctttttct tgatagtctt attctagatt
     8041 acagcttcca taaaaaaatt gattcttatc aagttttacg atttttcttc tttgctttcg
     8101 tgtccaaaat acaataaacg catcgcgtta aagataacga tcaagatact agcttcgtga
     8161 acgaacatcc cactagccat gtaaataaac ccggcaaata agccgttgag taaaaataag
     8221 acgacaccaa tggcaatcac tacattttca cgggtattta aaacagtttt cttagccaag
     8281 cggtaggcat gaaccaacga ttcaaaatta gattgcatca agacaacgtc actcgtttcg
     8341 atagcaacat ccgttcctga tcccatagca atcccgatat cagccgcagc caaagaggga
     8401 gaatcattga ttccatctcc aataaaagca acgcgcaatc cttgttcttg gtattttttc
     8461 acaaattgaa ccttttcgtc tggtaataat tcagcatgga cttcatcaat gcctaacata
     8521 tcagctacat aatcagcagt catctgatta tcacctgtca gcatcactaa ctgttttgct
     8581 cctgcttgtt tcaacttagc taattgttca gcgacttctg gtcgaatcac atctgacaca
     8641 cctatgattt gagtgatttt tttgtcgatt gctacaataa tgaccgagct gcctgtttgt
     8701 tgtaattgat gtaaatcttg tacttgcttt ggtgtgagag aaagtttgtg agcggcaatc
     8761 aatttggtgt tgcccgcaca aactgtatga ccatcaatct cagctgtgat tccttgtccc
     8821 ttgattgtct gattatcttt gactgctaat tgttgaaaat caatgtcttt ctcctgagca
     8881 taatggatca cggcgcgccc cagaggatga tcggataacg tttctaatcg agccactaaa
     8941 gctaagctgt ccccttcatc ctctgtatag ttcttgatag tggatacagc agtagttcct
     9001 ttggtcaatg taccagtttt atcaaagaca aaagtatcga ttttggaaaa agtatccatt
     9061 gcttcaccgc ctttgactaa gatgccccgt ttcgctccat taccgattcc tgctacattc
     9121 gaaacaggcg caccaatgac taaagcgcca ggacaaccaa gaaccaaaac agtgattgct
     9181 aaacgaaaat cacgactaat cagaccaaca accaacgcaa taagtaaaac ggttggagta
     9241 taatactgag aaaatcgatc aatgaatttt tccgcatggg atttggtatc ttgtgcttct
     9301 tctactaatt cgataatttt ggcaaaggtc gtatcatctc cgaccttagt tgcttcaatc
     9361 gtcacgtagc catctgctaa aatcgttcca gagaagactt catctccact gattttgacg
     9421 acttgtcgtg attcaccagt cactgcggct tcatccgcat accctttccc gtcaatgact
     9481 cgtccgtcca caggaatagc tccaccagtt tttactaaga cgatatcgcc ttcttccacg
     9541 tcatcgattt ccacttcttc agtcgttcca tcttcttgga caacactagc agtagatggg
     9601 gccatctcgg tcaaagcctt gattgactcc cgcgttttgg tcaatgtttt ttgttccaga
     9661 tagctaccaa acaaaaacaa gaatgtaacg atagctgact ccttgtattc tccgatcata
     9721 aaggcaccaa gcacagcaat agtaacgagt aaatcaattg agataacttt gactttcact
     9781 gcctgatacg catggatcat aataggtatg accgcaataa tagaagctat ggctagagca
     9841 atgttataga acaagacaaa accagtaaat tttccgataa aaccaaaaat aatcaacaca
     9901 gctgataagg cggtgatatg atttcgatgc tcttggatcc acttttgtaa tttcatcttt
     9961 ctccctcttt tcctttcatt cttacgagac taagtataga tacttatcca tcataaaaaa
    10021 ttgaccacta tcaagtttag tcaaaaaggc ggttttcttt tggtagaata cactacaaaa
    10081 aatagttagc aagaagactc actaaaagtg atcttgccaa ctattgtcgt ttctatcgaa
    10141 attttcattg aatgattttc ctgtacagca tacatcggag atctatttct ttatctaaga
    10201 tttttatctt gctaattttt tcctttagtc tttcaaatcc acgtcgttca tagaattgat
    10261 acgtacagcc actatctgta aaaaggaata tttcttttcc tttctctctt ttttcaaatt
    10321 ctgttagcaa cttggtaccg atgccttttg ttttcaaatc aggatttgca gctaaaaaaa
    10381 tgatctctcc atctggcgaa tgcttcttta aatatgctgc aagcatttgt tcatttgctg
    10441 tctcgtaagc tcctttactt tctttagcaa atatattttg taaaaaatcg aatatcttaa
    10501 catatatttg ctgtccaaaa gaacgatgtt tctttttttc tccttttatc tccgccagca
    10561 aaacacctgc caactcgtcc tctgcataca aagcaagaat ttgagttgct cgattcaact
    10621 ccatatacaa aaaatatctt ccgtaaagat tcagcagaag ttcattttcc atatatctct
    10681 caaagttcat tccttcaaca gcaaaataga tagctttttt gtaatctttc ttttctaagg
    10741 gtttaaaagt tagtttcatc atttcgcctc ctcaacttca atgattgtgt tatacttaca
    10801 ttataataga cacagtgtct actatctata atcaatcatc tataagtttg tcgattaagc
    10861 gacagctttg ctttgatatg tcggataagg agataggtaa atgaaaaaga cagatagacg
    10921 cgtcaaaaaa acagaaaaag ctttggctga aacactatcc acattattag taaataaaaa
    10981 gattcaagca atcactatac gcgaactaac agaaacggca gatgtacatc gttcgacttt
    11041 ttatacacat tataaggata tctatgatct atatgatcaa ttagaaagca acttttttcg
    11101 ggatctaaat gaaattctct cttatgatcc agcccatagc tatgaggaac tatacactcg
    11161 cttgattgac tatgtttacg caaatacttt tttatatcgc ttatttatcc tcaaccatgg
    11221 tgatatttcg tttcagaaga aagttaaaca agttattgaa gaaaactatc tgaacatatg
    11281 gctatatgaa gacaagaaaa cgattatcac agaagaaatg agattcttta ctacgtacca
    11341 cgtccaaggt tgtctatccg ttctcaatcg gtgggtagaa agcaattaca agtactccaa
    11401 aaaagaatta ttagaactct tacagaaact gaatgatcac atagagaaaa tcatgccttg
    11461 aataaaaaca tactaagctt agttaagaag aaccgtaacc ttctcgtata acaactgaat
    11521 cttatttagc caaattgttt actgaagatt ttttgagtaa aatttctata attcctactt
    11581 attgaaaagt agtttgtagt gctgcttctc cctctctaag tgatatatct tgttgtacca
    11641 atcaaattca cctcatcatt aataaattac ttttaggcta atttattaca acttataggt
    11701 tgaagctgta accaatttat agtcatatta tatttttatc atccagttta ttgaattctt
    11761 ctcgtttacc tcaattcatt ttcaagggga atcagctca
//
LOCUS       DACDIH010000076        11736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.076, whole genome shotgun sequence.
ACCESSION   DACDIH010000076 DACDIH010000000
VERSION     DACDIH010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.076"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(283..2358)
                     /locus_tag="IV281_002263"
     CDS             complement(283..2358)
                     /locus_tag="IV281_002263"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP8781234.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRVSRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQITAMALSL
                     ASLDQIITK"
     gene            complement(2360..3277)
                     /gene="glyQ"
                     /locus_tag="IV281_002264"
     CDS             complement(2360..3277)
                     /gene="glyQ"
                     /locus_tag="IV281_002264"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP8781235.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3661..4473)
                     /gene="recO"
                     /locus_tag="IV281_002265"
     CDS             complement(3661..4473)
                     /gene="recO"
                     /locus_tag="IV281_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP8781236.1"
                     /translation="MSQLVESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4616..5515)
                     /locus_tag="IV281_002266"
     CDS             complement(4616..5515)
                     /locus_tag="IV281_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP8781237.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5530..5931)
                     /locus_tag="IV281_002267"
     CDS             complement(5530..5931)
                     /locus_tag="IV281_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP8781238.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5909..6385)
                     /gene="ybeY"
                     /locus_tag="IV281_002268"
     CDS             complement(5909..6385)
                     /gene="ybeY"
                     /locus_tag="IV281_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP8781239.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6402..8594)
                     /locus_tag="IV281_002269"
     CDS             complement(6402..8594)
                     /locus_tag="IV281_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP8781240.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEVQEE"
     gene            complement(8618..9589)
                     /locus_tag="IV281_002270"
     CDS             complement(8618..9589)
                     /locus_tag="IV281_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP8781241.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10295..11674
                     /gene="brnQ"
                     /locus_tag="IV281_002271"
     CDS             10295..11674
                     /gene="brnQ"
                     /locus_tag="IV281_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8781242.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3204 a         2452 c         2140 g         3940 t
ORIGIN      
        1 catagaaata tttataaaca ggttttccct atataatcat accacaaaca gttttcttga
       61 ctagggaaat tcctaattta aaagtaaata aaacagtaac aagattatta ttaatctttt
      121 tcatttttat ttcctcactt ttcatgagcg ttttgaaaat tctagcaaag aataaaataa
      181 caaaaattat taaaatagaa ttaataacag cttttatatt tataacaaaa aaacagagac
      241 acagcacgtt tcgtgcatgc tctctgtttt tcaaacaaaa gactattttg tgatgatttg
      301 atccaagctt gctaatgaca aagccatggc agttatttga ctaagttgtg ttagtcggtt
      361 cttacgtact gctggttcat cagccatgac catcgtttga tcaaaataat tttcgatcag
      421 tggacgaaga gcgacaagct ggtcgtagtt ttcgtggatc gtttggtcag caaaacttgc
      481 tttcattgct tctactgctt tgtgtaattc tttctctgcg tcgttttcga acagctcagg
      541 gtctacttct gattgttttt caagctgcgc ttttttcgct aagttcacga cacgtgtcaa
      601 tgcttccatt gaaggtttga agtcttcatc tgcaagatgt tgtttgaaca attgtgctgc
      661 tgcaaatact tttgtcagat ctttttgttc tgcattcaaa acagcttcga tgacatcatg
      721 acggatattc ttcgttgtca ataattggcg cagacggcct ttgataaagt cgatcacctg
      781 ttgttgtccc gctgacaagc caatgccaaa acgatccgta tctttactga taacttcact
      841 aatcgtttct tgcagaacag acaaaggaaa ggcccatttt ttcgattcga tgattcgaac
      901 gacaccatat gcttgacggc gcaaagcata tggatcgttt gagccacttg ggatcatccc
      961 aactgcaaag aaagaaaaga tactatctaa tttatcagca atcgctaaaa cagaacctac
     1021 tgtagtctca ggtaaatctc cttcactgga tataggcatg taatgttcac ggatcgctgt
     1081 tgctacggct ggtttctcgc ctttaagcag agcatatttt tctcccatga tcccttgtag
     1141 ttcagggaat tctcctacca tgttggttac tagatcaaat ttatagattt cagcagcgcg
     1201 ttgcagatcc atcaattctt cctcagatag tcctacttgt tcgcctatga tgcgagcgat
     1261 gacttgtacc cgttgcattt tctcatagat gctaccgatt ttttcatgga aagtaacagt
     1321 ttttaatttt tcaacgtatt ggtcgattga agctttcttg tcttcattat aaaagaattc
     1381 cgcatcttct aaacgagcag tcaatacttt ttgattgcct cgtaccacat tttccaattt
     1441 tacccgatcg ccattacgag cagaaataaa atgaggaagc aacatacctt gatcgttccg
     1501 tacttcgaaa taacgttgat gttctttcat agaagtgact agtacttctt caggaacaga
     1561 tagatatttt tcttcgaagt cgccagaaaa gacagttgga tattcgacta gattgttgac
     1621 ttcttctagt aaatcttcat ctagatcaat gatccagtga tttttttctg ctaattcgtt
     1681 tgcttgtgtg atgatcattt cttttctttt ttgcggtgaa gcaataacaa attgttcatt
     1741 caatttttct tcgtattctt ttgcatgcgt aaatgtcaca tcttcgccta agaaacgatg
     1801 acctcttgaa actctgtctg ttttgacatc caagatctca aacgggatta tttcgtcatc
     1861 taacaatgcc acgatccagt gaatagggcg aatatattca aaatcgtatt ctgcccagtg
     1921 catcgttact gggaaagtga ggcttttaat gacatctgct aaacctgtca atacttcttt
     1981 tgcttctttg ccgtgagtgt atttcgttac atagacatat tccacgcctt ttaattctct
     2041 aaaagtgata gcatctgtcg tcagaccttg tccgcggaca aatccttcag ctgctttcgt
     2101 ccagtttcca tctgcgtcca aagcaatttt tttggctggt cctttgactt cttcttcgct
     2161 gtctgcttga cgttctggaa tgtttgttac tttgattgct aagcggcgcg gtgtagaaaa
     2221 cgtttcgatc gtatcataag tcaattggtt ttcatctaaa aattttgctg ttttttcttc
     2281 taattgtttc atgcttggtg tgactacatg agcaggcatt tcttccagcc cgatttcaag
     2341 taataagtct tttgccatct tattcagcct ccttcacgtt ttctggattt gctgtgtttt
     2401 tattcaataa agggaatcct aatttttcgc gttctgccac aaagattttg gctacagcac
     2461 gagccatgtt gcggatacgg gaaagataac ctgcacgctc tgttacggat actgcaccgc
     2521 gagcatctaa taaattgaat gtatggctac atttcaaaat ataatcatac gctggatgaa
     2581 caagattttc atcgatacag cgtttagctt ctttttcaaa acgatcaaaa ttgcttagta
     2641 gcaggtcttg gtcgcttact tcaaaagaat atttagaatg ttcatattct ggttgtttga
     2701 agatctcacc atatttcacg ccatcagccc attccagatc ataaacactt tcaacttctt
     2761 ggatataaga agcaagacgt tctagtccat aagtgatctc agaagtaact ggtttacaag
     2821 gtagtcctcc gacttgctgg aagtacgtga attgtgtgat ctccatccca tcaagccata
     2881 cttcccagcc cacaccggca caacccatcg atgggttctc ccagttgtct tcaacaaagc
     2941 gaatatcgtg ttctaatgga tcaattccta aaagacgcaa gctttctaaa taaagctctt
     3001 ggatattttc aggtgaaggt ttcattacga cttgaaattg atgatgctga tacaaacggt
     3061 ttgggttttc tccataacgc ccgtcagctg gtcggcggga tggctctaca taagcagcat
     3121 tccatggttc cggtccgatt gcccgtaaga atgtatatgg gctcatcgta cctgctcctt
     3181 tttctgtatc ataagcttgc atcagcatgc agccgtttga tgaccaaaat ttttgtaatg
     3241 ttaaaatcat atcttggacg gtcaattttt tactcatctg ttttgcccct ttcaattttt
     3301 aagcgttgct gtcaataaga atacaggaaa gaaaacgaca aaaagtccct atgtctgatt
     3361 acagacatag ggacgaataa tttcgcggtt ccaccctact tccgaataaa gattcggcag
     3421 cttcatttat cgtactcgca agtgcctctt caaaaatcgt ttgggtccgg cttccactct
     3481 cccgaactcg ctaaaccacg tgttttttac tgatcttggt caatgtacgt attcattttg
     3541 cagttttctg gtttgtataa tagatcctct cagtgaacga tctatttaat ctacggttaa
     3601 taatagacgg aagtccctgt atttgtcaaa tagttttgat ttttttgttg tatctttcat
     3661 ttattcttct ttttcaggta tctctttttc aggcatattt ttctctggcg tctttaatac
     3721 gtgtgcccaa gacttcattt gatcaatgaa ctttttgctt tttagatgaa taccgacata
     3781 ttcatcatac aactggtcaa tgaggtcacg gattgcctgc ttcgtttctt ctttcaagtg
     3841 gattccactg atcttatcat aagaaatagc cgaaaacatt cgaacgaaat aaacagcgcg
     3901 aggatctgca tgataacggt gttcgtccat tggccagtgt ttctcgcata tcacaccgcc
     3961 atatttggaa gaatagtcaa attttccttt tgtttccccg catacgacac agtgcgtcca
     4021 aattggcgcg attccgaagc gttgcagtat ttgcacttca aagatattcg tgatgatttc
     4081 tgcatcatct ccttggtcca tccgctgcag agcttgttgc gtaaattgga ataaatgcgg
     4141 gtcatattga tgatcttcga ttgctgcatc tactagattt agcaaatacg taccataacc
     4201 gctcaaaaag atatcttctt ggatgttccg aaacgcatgg acgtctttga cactgcttaa
     4261 aaacgacaaa ccttcacttc gaaaattacc gatatagact gcttcggtaa aggggagcaa
     4321 agccggagct aatggctgat tttgccgatg gattcctttg atataaaaca tcattttgcc
     4381 agaagattcc gtaaagattt tgactaattt atctttttct ttgtaattac ggctagataa
     4441 aatcaagcct ttcgattcta caagctggct catattctcc ctcctaaaaa ataaatctgg
     4501 aaaagcagat tctgccgatc cagattattt atgtttcgat cttccgctgt ttttctcaac
     4561 gtacggattt ttatccagtt tatttctata cctaacctat aagaagtcca gaatatcagt
     4621 actcgtcttt tcggtagccg aagtcttgca gatagacttt tttgtctcgc cagtcttttt
     4681 ggactttgac ccaaagttct agaaatactt tgtctcctaa aagattttcg atatcctttc
     4741 ttgctttcgt tcctacttgc ttcagcattt ttccgccttt gccgataatg attccttttt
     4801 ggctatctct ttcgacgatg atcgtggcct ggatatggat tttatcattc tcgtttcgct
     4861 tcatcgaatc aacaacgact gcgacagaat gaggcacttc gtctcgtgtt aaaaacaaga
     4921 ctttctcacg gatcaattca gaaacgatga agtattcagg atgatcagtc acttgatcat
     4981 ctggaaaata ttgtgggccc tctggcattt cactgactaa tacatccatt aagcgttcga
     5041 cattgtttcc ttctgtagca gagatcggaa tcacttgtgc aaaaggcatc tgcgtcgaat
     5101 aatcttcgat gattcccagc aactcgtctg gatggatctt atcgatttta ttgataatca
     5161 gataaacagg agaatgcacg ttttttagac gttcaataat aaaatcgtct ccccggccac
     5221 gtttttgatc agcactgatc ataaataaga ctgcatccac ttctttcaac gcactgtaag
     5281 ctgcttctac cataaaatct cctagacgat gctttggttt atggattcct ggtgtatcga
     5341 taaaaatcag ctgagcttct ggtgtagtat atactccttg gattttattt ctagttgtct
     5401 gtgccttgtc actcataatg gcaatttttt gacccacgat tcggttcaat aatgtggatt
     5461 ttcctacatt cgggcggccg acgattgcga caaaccctga tttatgttct ggcatatacg
     5521 ttctcctttt taattaaaca acaaatgata tattttaggg acaaataaaa tcaacccaat
     5581 gatgacagca aaacaagcgg tcactaaaac tgctccagct gccatatctt tgattttttt
     5641 cccaatcgga tgaaaatgaa aatccgtaaa catgtccact acattttcaa aaacagtatt
     5701 gatgatctct actatccaaa cgagaaaaac agctaaaagc agccataacc actctatccg
     5761 atcaagctgg aaaatcacgc ccataaataa agcaaggata ccgaacgcaa catgtttcct
     5821 catattgcgt tcttcctcaa aaacggtttt gactccttgg acagcaaatt ccacagacat
     5881 caggaaatgt ttgtttttct ctacgttttt atcttttgag tccataggca tccaagatct
     5941 ctttctgtaa accaaacatt tctttctcat cttctggtgt catatggtca tacccgttga
     6001 tatgcaaaaa tccatgtacc gctaaaaagc caagttcccg atcatatgaa tggccgtaag
     6061 cttctgcttg ttcagcagca cgttcaatag agatcatgat gtctcctagt acacgaggta
     6121 atggattctc tccatcttca aaaatcactg ggatttcgtc ttcgccttct tcttccagag
     6181 caaaactgat gacgtctgtc ggtgcatctt ttccacggta atcacgattg attacttgaa
     6241 ttgccgcatt gtccatgaaa gtaacagaca tttctgtttc ctctggcagc tttaaataag
     6301 atgctgcaaa ttgcagcaag tcatcgattt ctttcatttt ctcttctgag acttggtttg
     6361 tttcatccat aaaggtaata tccattatac atcgcttctt tctattcttc ttgtacttcc
     6421 tttatcttct caacttctgt tttcatttta ggatatttga tccgagaatg gaaggtgcta
     6481 cttaaaccgc tgattaatga tttttccact tttcggattt ctttcaaggt cagtccacta
     6541 tcgtctaatt gaccatcgga aatacgctct tcgataagat tatggacaaa actttcgatt
     6601 ttttcgctcg tcggatgatc cattgcccgt actgcagcct cacaactgtc agcaatattg
     6661 acgatccctg cttcacgact ttgcggtttt ggtccaggat aacggaattg ctcttctgtt
     6721 acttccggat tgcgttcttt cgctttgatg tagaaataac gcattagcgt ggttccatga
     6781 tgctgtttgc aaatatcgat caccatttgc ggcatattat aatcttccaa tattttggct
     6841 ccatctgtca catgtccaaa gataatatgt ttactgtctt ctggcaataa gaaattgtgc
     6901 gggttttccg ctcctgaagg caagttttca acgaaaaagc tagcatgttt gatcttgccg
     6961 atatcatgat aatagcaagc tactcgagtt agcaaagaac gtccgccgat ttctgctaca
     7021 gcgttagcac ttagattagc taccatcata ctatgatgat aagtacctgg cgcttcttcc
     7081 agcaactgtt tcaataaagg atgatttgga ttgctgagtt catttaatac aattacactg
     7141 tcatcattga ccatcaattc aatatatggg tgtagaccga tcgtcagcaa gaatgagaag
     7201 agtgcaccag tgaacccgca gacgagcatt aaccaagtcg ttgcatcacc aaagctcatt
     7261 ccttgataga cgatcaaaat gacatccatc atcacaggaa aaataatgac ccacatggca
     7321 gcagataatc cttgttctga gatccgtttg cgtctgacaa tcgttgccat gagcccagag
     7381 aaaacataag acatcaagat aatcgttaga aagttcgtgc caatcgaatc ataaaaaatg
     7441 aaaagtgcgg atactgcttg gaaaatacca gccatgatcc ctgctcttcg attcaagaaa
     7501 aagcttaaaa caagtggtgc aaaagcagcg ggataaaaca gcgggatata agtagcttgt
     7561 tccgtttgga ataattggaa aaatttcatc agcactatgc tgactgacat agctgtcaca
     7621 tagaaaagca cgtattttgt tctttctcct gcattttgga attgaagtga attataaatc
     7681 agtacgccga tttgaagcaa aacagctaaa accatggcaa ttagcggaaa aatcgaagtt
     7741 gtttgattag tcagtcctag taattccaat tttttcatag ctgaagcatc gatttgatta
     7801 ccttcacgaa caatgatttc tccttggaag atcatcactg gttgtacagc ttctcgtgcg
     7861 gattgtttca attcttctgt ctttttctca ttcaatacgt cattcactac gataccttga
     7921 tccactaagt agcgaatcat ttgttgttgt gctggtgtga cattcaaata ttggatttgc
     7981 tcttctgctt cttgtttaaa agcttccaat tcactttcac gaacttgttt gctcatctgt
     8041 tcgtcaatga gcttcagact ctcatccctc acagtatgaa tctcagtttc agtcatttca
     8101 aatatcgtcg aataaaaatt gttcggtagt ttttgataaa acacaacatt ttctgcattc
     8161 acattttcaa aatttttctt cagcgcggcg atccgctctt ctgtagtagg ctttgggata
     8221 ttttcatctt cctttgcctt atcaatcttt tcgtcataag atttgttgat ggcatcattc
     8281 gtcttatcga tcaattcaaa tagttgcttg atccgattgt tctggtcatc tgcaagatct
     8341 ttttggtaag tgtattctgg agtgactgct tctgctgcca gttttctttt ttgttcggtt
     8401 tcttccgtgt tctcgatcgt tttgttcgca cggatacttt cttctgctac ttggccttct
     8461 ctgaaatcat tggccttttg tttgacacta ctaaatgtaa cgccgcagat aatgataaaa
     8521 aacaagaaca gcaggaaagg caatgctgcc tttccaagtt tttcttgtag tttctgcagt
     8581 gttttattca acatgtgctt atcctcctaa gcaaagctta ttcttgatgg agatctgcct
     8641 tttcataagc tttgatgatt tcggcaacta cagggtgccg aacaacgtca cttgcttcaa
     8701 aattaacaaa atcgattttc ttgatttgct ttaatgttcg ttctgcatga acaagaccac
     8761 ttgtcgtccc tcgaggcaag tctatctgac ttgtatcccc attgacaatc atttttgatt
     8821 gataaccgag acgggtcaag aacattttca tctgagcgac tgtcgtgttt tgtgcttcgt
     8881 ccaagataac gaatgcatca tccaatgttc gcccccgcat gtaagctaaa ggtgcgattt
     8941 cgattacgcc tcgttccatc aatcggttcg tatgatccaa tccaaaaatt tggtataaag
     9001 catcataaac aggccgcaga taaggatcca ctttttcttt tagatccccg ggaagaaagc
     9061 ctagattttc acctgcttcc acagcaggcc gcgtcaaaat gattttttgc acttcacctt
     9121 ttttcaaggc agcgattgct aatactactg caagaaaagt ttttcctgtc ccggctggtc
     9181 ctacgccaaa tacgatatca tgtttggcta ccgattcgac atatttcttt tggccgctgt
     9241 tttttacccg tataggtttt ccattacggt ctttgacgat ctcttcctca tacatctcaa
     9301 caaaataatc taatgtacct ttttgcccca ttttcaatgc cgtcacaaca tcgggcgtac
     9361 ttacgtggat tcctcgtttg atcaattcct gtaatgtccg aatgactgag gcagccaagg
     9421 aaatctggga ttgctcacca atgatctgaa tgatttctcc acgtgtatgg atggttgttt
     9481 ccgttgtttc ctcaatcagt tttatatgct tgtcgtgtgc acctaaaagc atgctgacat
     9541 catcagcagc cgtcaatcta atctctaaag aactagactc ttccgtcaaa aatagacatc
     9601 cccttttttt cgattaattc tttcctattt taccatactc agaagaaact atatagcaaa
     9661 taagaaaagg ttctaaaaga gttgcttaac cataagtacc aagaaacgtt aaaaaactag
     9721 ccgcttccat tctctaaagg tctcgtttga ataaaaaaag ctgatcatgt aagctatgca
     9781 taagatagag gacaagtatt ccgtctaact tagcaagtat tctctgggta tttttcttgt
     9841 ttcaaaaaaa gaaaacaaaa tatttgaaat attcttgcat ttcaaaaaaa acaggtgtat
     9901 tataacgtca acaaataaat atacagcaaa gcgtcgagga aaacagtagg ctattcagtg
     9961 atttattccc agtgagtcat gtaagtgaga aatgacaata aatcgaatag acgaacatca
    10021 tttccgagtc aatgtcgaaa acgtagatca ttgtggctgc ctccattatc aggctagcgg
    10081 ataactttat cgaaaagcgt ccgtgaatga agaagctttt ttaagcgaaa aaaggtggta
    10141 ccgttctaga atctagagcc cttttcccaa gtggaaaagg gcttttttta tattttaagg
    10201 tggtggtttt catggaatct gatattagtg gtatttctag tttctgcaac gtttattttg
    10261 ttactcaaaa tgataattat tggaggaaaa atttatggaa gaaacaaaaa cttattcatg
    10321 gaagcaatta ttgatggtca gttcacttat cttcgggatg ttcttcgggt caggtaatct
    10381 gatctttcct gtacacttag gtcaaatggc tggatcaaac tggatcagcg ctggtgtagg
    10441 atttgccatc tcaggcgctc ttttcccatt attagctatc ttagctgtag tagtgacaaa
    10501 aagtgatggt ctgtatgact tagctaaacc tgtcggaaaa tggtatgctg ccttgttctt
    10561 gatccttgtt catttaacga tcggaccatt ctttggtaca ccgcggacag cagcaactgc
    10621 ttttgaaatg gctgctaaac cctttttgcc tgaaaaatat gattcattag gtatgttttt
    10681 attctctgct cttttctttt tacttgctta tttccttact gtacatccaa cacgtttggt
    10741 caaatatgtc ggaaagtatt taaactcctt gtttcttgca ttattagcta tcatttttgt
    10801 cgtttcttta ttccaaccca tggggaatct aaaccaatct gctgcagatt cttatcagac
    10861 gaacgctttg acaaatggcg ttttagaagg atacaacacg gttgatgcag tagctttact
    10921 tgctttaagt gtcacttttg tccatgcagt tcgtgatctt ggttttaaag ataaaaaagt
    10981 cacagaatta acagcaaaag ctggtagcct gagcgttgcg ttagaagtca tgatttattt
    11041 tggtctggtc cttctcggtg cgatgagttt gaataagatg tctttatcag aaaatggcgg
    11101 aactgcactt tctcagatca ctaattttta tttaggtaac ttcggtgcta gcttcttagg
    11161 ggtaatggtc acattaggtg tcttcactac tgctatggga ctcgttgttt cttttgcaca
    11221 agatttccat aaactgttcc ctaaagtcag ttatatgaca tggctgcggt taacgacttt
    11281 tgtctctttt gtcgttgcga atgccggatt ggataatatc atccaatggt ctttaccagt
    11341 attgatgctt ttgtatccat tgtcacttgc gttaatctta ctttccttaa cagcaaaatt
    11401 cttccaaaag agtccatttg tctatcaagt aacgatgttc ttttctgccg ttccagccgt
    11461 attggatatg ttggcaaatt cgcctgcatt agtgagtcag caaactgtcg tagcaagctt
    11521 actaggattt tatcatcatt atgttccttt tgctgcatta ggtcttggct gggtcgtccc
    11581 aacattgctt ggttacgctg gcagcttgct cttttattat gtatatcgcg tatcaggata
    11641 taaacaagaa gcaagtgagt taccagaaga ataaccaaat caaacacaat ttaacataag
    11701 agatattaaa caggaaaaaa acagctagaa gaaaga
//
LOCUS       DACDIH010000077        11710 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDIH010000077 DACDIH010000000
VERSION     DACDIH010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.077"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..591
                     /locus_tag="IV281_002272"
     CDS             <1..591
                     /locus_tag="IV281_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8781243.1"
                     /translation="VVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEMQTVVKK
                     CGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSVGEDFSL
                     AAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGKFYHEA
                     IVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            611..973
                     /locus_tag="IV281_002273"
     CDS             611..973
                     /locus_tag="IV281_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781244.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            976..1158
                     /locus_tag="IV281_002274"
     CDS             976..1158
                     /locus_tag="IV281_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781245.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            1175..4606
                     /locus_tag="IV281_002275"
     CDS             1175..4606
                     /locus_tag="IV281_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8781246.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIDNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            4657..5394
                     /locus_tag="IV281_002276"
     CDS             4657..5394
                     /locus_tag="IV281_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8781247.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            5404..7695
                     /locus_tag="IV281_002277"
     CDS             5404..7695
                     /locus_tag="IV281_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781248.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            7719..9845
                     /locus_tag="IV281_002278"
     CDS             7719..9845
                     /locus_tag="IV281_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781249.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            10008..10454
                     /locus_tag="IV281_002279"
     CDS             10008..10454
                     /locus_tag="IV281_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781250.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            10456..10593
                     /locus_tag="IV281_002280"
     CDS             10456..10593
                     /locus_tag="IV281_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8781251.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            10631..10924
                     /locus_tag="IV281_002281"
     CDS             10631..10924
                     /locus_tag="IV281_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781252.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            10921..11145
                     /locus_tag="IV281_002282"
     CDS             10921..11145
                     /locus_tag="IV281_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8781253.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            11142..>11710
                     /locus_tag="IV281_002283"
     CDS             11142..>11710
                     /locus_tag="IV281_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8781254.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLA"
BASE COUNT         4087 a         1809 c         2488 g         3326 t
ORIGIN      
        1 gtagtaacaa cgttcgataa cgtaagtatc aaacgaattg cttttaattt taagaacgca
       61 ggaaatgcaa tcgcaacgaa ttgtaatgga caattagatg gcgaaacaga aatgcaaacg
      121 gtggttaaaa aatgtggagc gacagaagta aaatcaaaat ctaaaccaat caatatgacg
      181 gtaacaatta ctgcacatgt accgatggaa gtttatcgac gtttcaatgg gttgaaacaa
      241 gatggacgta ttaaaccagg catttactct tacggtcctg attccgtagg cgaagatttc
      301 tcacttgctg cagagatcgt ggatgacttc gaagaaaata gaaagttagt tggtatgtta
      361 gcatgcactt cgaatacagg attaacattc tctattgaaa atggtgcgga tgaagtagct
      421 gcgttagaac tagaaacaaa agttatgcaa gatgaatttg gtaaatttta tcatgaagca
      481 attgttgcag aacttgaaga agacttaaca gatcaatgga tgacgaatct atctgctgat
      541 gtgattaaaa aaacttcagt tgtgacaact acggccactc aatcacagta aaaaaaacgg
      601 aggtagcgaa atgaacgaag attactcaaa aattgaacta aacgatggaa caattttgaa
      661 tttagaacct aaactgaata tcaagaaatt attgatgatc aatagagatt ttaacacaga
      721 cgagtttgca aaaatgactg tgggaaaagg atccatggat atttctgtta ttcaaggtgc
      781 aaaggctgtg tatattgctt accgccaagc gaacatgact gattatattt cattcgatga
      841 atttatcgat aaatgggatt ttgatatggc tactgccagc tatatttatc aattgatgat
      901 gttcaaacaa gcacgcgatg cttatcaaaa agaatttgaa aaagcaaata aggaaaaaaa
      961 gcttcaaaag taaaaattcc aaagctctta gttgaaacgt gggtcgatgt ctattcgatg
     1021 ttgaccgacg ttttttctat gccttcagat ttggttttaa gcgatatctg tttagatgac
     1081 attttgcaaa tggcttacaa caagagtgct tatgaaggat ggaaaaacta tgcaataaac
     1141 caatcccaga aaaactaaag aaaggaggta aaaaatggct aaaaagagaa cagaagcaga
     1201 agtaactttc atagctaacg atgacggatt gaaatctacg ttaaaagaaa tcagtgctga
     1261 attaactaaa aatagagcag aattaaaact agaacaagct caattacaac agactggttc
     1321 tgaatcagac aagttaggaa gtaaattatc ttctttagaa aagcagtatg aattacaaag
     1381 tcaaaaagtt gaagtaacta gccaacgttt agccaatgcg aaaaaatatt atggagaaaa
     1441 ttccaccgaa gttcagaaac ttgaaagaga actgattaat caacaaacag cgcaacaacg
     1501 tttgtcaaac gaaattgata aaacgagtaa tgcactagct caagcaaaag gcgaaataca
     1561 gacgtacgag tctacaatgc aacagttgga tagcgaacaa aaaaatgttc aagctagtgc
     1621 ttctctgatt gaatccgaat acaaaaaatg gcaagcaact gctggtcaat cagcttctga
     1681 agccgagaaa ttagcgaaag cccaagaata tgtttctcaa caatctgaaa atgcggagaa
     1741 aacgatagat atcctaagac gacagttaga agctacacag tctgaatttg gcgctacatc
     1801 cacagaagca atgcagatgg aagcgaagct taatgatgct gaacgtgaat ttgaagagtt
     1861 aggacaagct gctaaaaatg tagatacaac taacttggac gatatcggaa gcaaaataga
     1921 tatgaataat ttaatggaag cttctgacgt tttaagcgac attggcgata agcttacaga
     1981 attagggaaa caagcagtgg actctgctaa cagtgtaggt agttcccaga gtaagataca
     2041 agctaatttt ggtttgtcta aacaagaggc tgaagaatta acgaatgtag ccagagacat
     2101 ttattataaa ggttttggag aatcgttaga tcagtccaca gatgcattga ttttggtaaa
     2161 gcgtaattta ggagatttaa ataatcaaga tttacaaaat atcacggaac aagctatggt
     2221 cctagaaaac accatgggcg ctgatatgga tgaaacgtta cgtggtgtaa atggcttaat
     2281 ggtcaatttc ggcttgagtg ctcaagatgc aatggattta atggtttcgg gtactcaaaa
     2341 cggtttagat aaaacgcacg aattaggcga caatatggca gaatatagcc aattatggag
     2401 tcaaatggga tattcagctg atgaaacgtt cggaatgctt caaaatggtt tagatgcggg
     2461 tgcttataac cttgataaag tcaatgactt agttaaggaa atgggaatat cgttaacaga
     2521 tggtcgattt gagcaaaaca tggatatgtt tagtgaaagt actagaaaag cttttgaaga
     2581 gtggaaaaat ggcggaggaa cacaaaaaga cgttattaat tccatgattc aagattttag
     2641 caatatggat ggtcaatacg accaattaaa taaagcttcg acaatttggt ctgcgcttgg
     2701 cgaagataat gcgatgaaag ttgtccaatc tttaactgat gttaaccata catttgatga
     2761 tgttagtgga tctgcacaaa aaatgaatga agattctact actccgttgc aagagttgaa
     2821 cgggaaaata gctgaattaa aggattcatt agctcctata gacaacacaa tcatagatgc
     2881 actcgaacca gtaattgatt ttctaggaaa gatggctgat gcgtttaata atcttccaca
     2941 accagtacag gatttcattg ttgctatagg tggtcttaca gcagcatttg gcatactgtt
     3001 accggcaata ctagccgtgt catatttatt tggtccgatg atgcttataa ttggaggaat
     3061 tatagctgtt atagcaggcg ttattgtggc aattaagaac tggggtgcaa ttactgactg
     3121 gtttagtggt ttatggaaaa aatttactga ttggttgggt gatacttggg aaagtataaa
     3181 agacggagcc tcatcagttt gggatggggt taaagaaacc tggtctggat ttgtagattg
     3241 ggttcaagat atttggcaag gagtttctga ttggtttgga gagctatgga gcggattagt
     3301 tgaaggagct tccaacatct ggcaaggagt ccaagaaact tggcaaacat tcgttgattg
     3361 ggtttcaaat atttggaacg gagtcaaaga agtatggtcg attatttggg cagacattgt
     3421 aggaattgtt caaataccat ggacattaat aacgtcattg attcaagctg gtattaatat
     3481 tatcgtgggt atttttgatg tagctggaca gttattgggc gcagcttggc aagctgtttg
     3541 gacacctatt tctgatttcc ttaaaaatac ttgggatact atgacacaat ggataagtat
     3601 cgcttggaat gggattgtaa ctacattcca tactatattt gatccagtag tggcatggtg
     3661 gaatggtata tggacagcta ttagtactac ggcttcaaat atttggaatt caattagtgc
     3721 aacagcttct agtatttgga acagtatcaa gaatacaatc actagcttgg tacaagcagc
     3781 tgctacagta attcaaaata tttggtcaac tgtatctagt tggttaggtg gaatttggaa
     3841 ttcaatcagc tctacagcat caaatatctg gaatagtgtg actagtagta taagcaatgc
     3901 tataaacgca gctaaaagtg ccattcaaag tgtttggaat agtatatctt cgtggattag
     3961 cggaatttgg aacggtatca aaaatactgc tttgaatctt tggaatggaa ttacaagcac
     4021 tattagctct aaagtaaacg atggaaaaaa tgcaatttca agcggttggt ccaatctaac
     4081 aggtattgtt tccgacatat tcaataatgt taaaagtaca attgctaaca tatgggaagg
     4141 catcaaaaag actgttagcg ctccaattga ttggattaga gacaaaatca gtggcatttt
     4201 tgataatttg aatatttcta taccacatat tccgttaccg gaatttatca tggaaggcag
     4261 ctttaacccg ctaaaaggtc aaatccccca tttgcgtgta aaatggcatg ctaaaggagg
     4321 tatctttact aaaccaactt tactaggtgg aatgaacggt gtcggtgaag caggacctga
     4381 agcagtttta cctttgaaaa gatctgtttt gcaagaaatt ggtgatcgta tcttgagtag
     4441 cacatcagtt tcatctaggg cacaaacgat tcaacctgtg aataactacg aattcaattt
     4501 cacaattgat ggtaacgcag atgaggttac tatgaagcaa acaactcaac aaatcattga
     4561 tagcattaca aaaattcaaa atgataatgc ttcggcatgg cgttaaacag gagagtattt
     4621 ctcctgtttt tttagtatta aaaaggatgt gaaaaaatga ctgattgtat acattctata
     4681 atcgatggat ttcctgatta tttgcataaa ttggctttag ctgaaagacc aaccatacct
     4741 tctccaaaaa gacagagagt tgaaacttct gttttaggta ggttaggtgg cttagtacaa
     4801 gattactcgt ttgaagatat gtcgtttaca ttgcactata actatttaga ggatgtggaa
     4861 gaccatcaag cgttcaagca atcgttttat atcatgcgtc attggttaaa ctatgcaaag
     4921 aaattagaat tctctgacga tcccaacgtc tattacgtta tccagactat cgatattggg
     4981 gatgcagaaa acgatattgt tgaatgggga gagttcgatg taaatattac tgcgaaacca
     5041 ttcgcaagag ttcaagaaga tgtacctata accgtagata aaccacagtc atttaacttg
     5101 ctgaataata gtttagaaga aagttttcca aagattatca tcactccttc agctacttca
     5161 tgccagttca tcttaaatga ttatgtgttt agtttcgaag gcttagtagc aggaactgat
     5221 gtagtcattg atagtgattt gatgctttgc tacgaagagc aatcggacgg agatatttta
     5281 gatcggtcca acaaaatgaa gaccatgcaa tatccgacat tgcaagtgga tatcaatcat
     5341 tttaattgca ctggtttgag caaaatacaa atttatcgta acgggttaag gtaggtgaaa
     5401 tagatgatcg ataatttaat aactatttac gataaaaatg acgcgaataa tttagctgaa
     5461 catttatatg atacgcaagg tttaggcgct ttgtcagact ggttaacagc tactgttagc
     5521 aataaactaa acggagccga gatatttcag ggtacttatt caataaacgg aactaatgca
     5581 gatttgattg tagacggacg tattattcag tgttatgtag atgaaaatcg agcaaaacag
     5641 cgtctacgga tttattatgc aaaaacttct gtaataggca atacgataga agtaaaagct
     5701 gaacctattt tcaatgatat aagaaaatcg gtgttgaata aatatgacag cggaacagaa
     5761 aagatcactg ctactcaggc atggcaaaac gcaaaagttt tagcgaaacc agctatccct
     5821 tcgcagtttt ctttctcgtc attagtagat acgcttgcta atgtgaagat agaaaaggcg
     5881 aattttttag aattctttgg tggaaaagag ggatctattc tagatcgatt tcatggtgag
     5941 tttctaaaag ataataacac attacgtcat gaaaaaagtc taggtacgga tcataaaatc
     6001 aaagcgattt atactaaaaa cttaactggt cttgacttag agattgatgc tcaaagtgtt
     6061 ttagttggag tttatccatt cattagcagc tcttcagaag gagaagacga gatcactcta
     6121 ccagaagaag ttattttcac ggattacgtg gatgattatc ctgctggata tgtttctttt
     6181 gttgatttta aagacaaagc gactgatgta gccacattaa gggaagctgc taaagactgg
     6241 ttgaaaacaa acatagataa acaaaaacca caagtgagtg gttcgattga attagtacca
     6301 ttgaggcatc aaagaggcta tgaaaaattt gttgatctag aaaaagtttc gatgggtgac
     6361 ggagtagatg tgtatcatcc acagttaaaa gtgaatatgt cagcgagaat tgtggaatat
     6421 acgtttaatg ttctaaccaa ttcatacgat aaattagttg taggaaacgt caaaacaaac
     6481 ttcttagaaa acacagagaa taatgtcagc aatttgatta atgatgccat tgatcaattg
     6541 aaaaacggtg gcgaaatcag tgatttactc aatgatattg tagatcatca aactgatatg
     6601 attactggtc aaaatggtgg ttatgtttta ttagatccta aagaagcgcc tagtcgtatt
     6661 ttgattatgg acacaccaga taagaatacc gcaaggaatg ttttacaaat caacaatgct
     6721 ggtattggtt tttctaaaac tggcattaat ggaacatatg aaacggcatg gacgttagat
     6781 ggcggattca atgcctcgtt tattacggct ggtgaaatag taggaattac tattagaggt
     6841 actacattaa ttagtgatgg tgctgattat agaacaagta ttgctaatgg caaaatgact
     6901 tggtactcaa aaaaagttaa caaagatatt atggagctag aagcacgtga ttatgtaagt
     6961 gctgatgccg gtattgtatc atacaccatg aaaactggtg gtggtttcat gattagaaat
     7021 ccacagggta acttggtttt tagtacgtgg gataatggta ataacagacc gtttctatct
     7081 tttggtgcgc ccaatttcag gtatagcaat gctagttatg taacttctgg cgacggtagt
     7141 tctttaagca ttaatggtag tgcgggtaac tcatgggaat ttaaggtagc tggtaggact
     7201 atgaaattta ctagtgatgg tatgctaacg ttaccaggtt gtttttttgg ttcatgggaa
     7261 gatgggaaac ttgctaggtt tgaacaatca acggtacaag tatataaaga ttttactgtt
     7321 agaggtacta aaaactcaac tgtaccaaca gaacattatg gacaacgact attgaacgct
     7381 tatgaaactc cagaatatta tttcgctgat tatggggaag ccgttacagg tgacaatggt
     7441 aaagttcgtg ttgatattga ccccatgttt gctgaaacag taaatctaag tcggtatatg
     7501 acacatgtga cacctacaga actagttttg tgtgctgtta ctcatgaaga tattgaccat
     7561 ttcatcattg aaactagtaa gccaaacgta ttagttagat ggaatttagt ggcacaccgt
     7621 ctagggtatg aagatattag attaaaagag gatacagcat atgatagcac agtgcttgac
     7681 caaaaacgtt tttaaaacga agacaaggag gtatataaat ggctagcagt ttatataatt
     7741 tggctttaga tttcagcaaa gaattaaact acaccaaagc tattatggct cgtcaaggtg
     7801 ataaagggat tacggtgacg gttaaaccgt ttctaaatgg cttgcagatg gatacgagtg
     7861 gcggaacatt tactttaaaa ggaacaacac catctaaccg ttacgtagat aatgttgcaa
     7921 ctagtgtaac tagtgaagaa gtcacgtttt ctcttgatgg cacatttatg agtgaagcag
     7981 gatattataa acactgctac gtagaatata gaaaagacaa tcaaatttta acaacgcaag
     8041 atatcatttt tttctcacta ggagtgtctg acatttcgca aggccaagcc gatgaatatg
     8101 tttcgcaatt agaagagttg attcgaaagt acaacgaaac ttttgatgct tttatggctg
     8161 aaatcaaagg tagagtggat agcttaaatc aacagattac tgatttaact ggtcaagcta
     8221 aaacgctaca agacaagtta gatgctctga aagaagaaat ttctaagtta ggtaacttac
     8281 aagtgatgta cagtaacagc atcgacttcg gggactatga ttattcggag aacccgaatt
     8341 taatgccata tattacagaa ccttgggttg gacctttgtt aggtaatgga catacagtta
     8401 aagatagtgt taaaagggtt ataactcata ctaaaactag aacagctaat tccggcgaca
     8461 tattatcgct tggcttaggt attccgtgta cagcggaagc taataatagg tatcttataa
     8521 ccactttacg accatctacg acatacacat taagcgttac tatgagtgtc ggttcagatt
     8581 ggactggaga gaccaatact ataggggtta ggcttagata tttaaatgaa caaggtggta
     8641 tagaactgcc cattaatgca ttaataccag caaatgtaga acgcgataaa atggtcactc
     8701 atacatttac tggtataaca aaagataatg taactagcat aacaaactgt tatgttgaaa
     8761 tattctcact taatagtgaa tataaaggaa cggttagtgt cagctatgac gttaagttaa
     8821 aagcacacta ccctaactta ctagatggcc cttactggtt aggtaaagtt cctttaggcg
     8881 agaatattgc tgaccctaca gttgtctttc cacacaaaac tagtgaatat atggtttatg
     8941 gtagacgcaa tacagaaaac tatatcgctg accaaacata taccatatca atgaaagcaa
     9001 ctaaactaac agttcaatcg tttgccgtgt atattgctgc tggccgcgtt aaagtaggag
     9061 acatgaagcc tactgagggg ttagctaata catgggagtt aacttttact gtaactaagc
     9121 aacatataga tagtggggta actaattatc tggaaatata ccaatacccg tcagctacaa
     9181 aaggagctgt tcaaattgag tggctcaaac tagaaaaagg caatactcga accccgaata
     9241 ttagtgagta taaatatcgt ggtactggta tgcgtgattc aaacaatcca aaagattatg
     9301 tttgggatct agcaccagaa tatgtcgaag ataatttggc cacagatatt aaaatttctg
     9361 aaattactgg taaagcaaac aattataccg atgggaaagt atcggagatt aattcgcagt
     9421 tgactgcttc aattaatgaa gtagacacca cagctaagga tgctcaaaca aaagcgaatg
     9481 ctaatgcgac tgctatagat gaattagaca ataagatcga tgaacgcatt aatgatacag
     9541 ctactaccac attaacagtt acaaacggga ataccggatc agcaaagctt tatcgtgaag
     9601 gaaaaacagt ttctatatat tttgtggctt taaacggaaa aagcagtggt ggaaatgatt
     9661 caacgatact aacaattcca gaaggctatc ggccaccaat tagttttgag caactggttg
     9721 gctcgataga ccgttctact ttgaacagtg ctcagttatc tattggtgca gatggagcca
     9781 ttaaatggcg aagaaactca agttatggat cggattatac ctttgcaatt acttacacga
     9841 tttagaaaag cgtgaatcga tatgaaggca gcatatagac caattgaacc ttacggattc
     9901 gagcaaatca ttgtgaatga tgaagaacat ttaccggaag aatgcacaga agtcgaacca
     9961 ccgattccaa attggaaacc gaaattcaat tactgggagg gaaataaatg aaaaacattt
    10021 ggaaatatgg acgtactggc ggagagtacg caggaaaagt attggacgac atgcttgtat
    10081 ccgttcctta cacagatcag ccaccgcttg aaggaattcg tgctgatggc gaaccgttaa
    10141 caatcgctga tcaaatgttt gatcctaaat tgaatcaatg gattatttta gcgaacgcac
    10201 tagatcacaa cgatttaaac aatctcaaag caatgtatga gtcgttagaa aatgagaacg
    10261 gcgatttaaa acagatcaat gccaaactca tgctaagcga tgtagcaatt aaacaggaaa
    10321 atactgcatt gaaagaaaaa gcggatagtt tagcacaaat caattcaaaa atgatgcttg
    10381 cttcgttaca aaatagcaaa gacatttcag aaattaaaga gcaactaaat ccagcttcaa
    10441 agggaggtga gtagtatgtt tagttttagc gatgtgaaaa tgatgtatga ttggggctgt
    10501 ttcactaacg aacaagtaat ggttttcgtt ccgttgtgca ttactgaaga aaaagcagat
    10561 aaaatcatta gcaaagaaga gagcgcatct taattgatgt gctttttatt ttgattcaag
    10621 gagttgtcac atgattaatt taggggaatg gggagcgata gcaggatcaa taaccgctat
    10681 cgtttctttg attttattag taataaaacc aattactgca tctttctcga agattactga
    10741 gactctttca aaagtaagtc acaatttaga tttgctgact aaagatttag aatcgagcaa
    10801 atcagatcga ttgatgattc atgaagaact aaagaaacac gatgaaagat tagatacaca
    10861 tgcagaaaaa ttggtagaac acacgcaaca aattaaaact ttatttaggg agagaagaaa
    10921 atgaataata aaacgttcga agtactaaaa tggttcgcac tggtaattat tcccgcacta
    10981 gctactttcg tggggttagt tggtaaagcg ctcaattggc agtacacaga tatctgtgtt
    11041 gtcatcatta ctggttttgg cgcgttttta gggagtgtgt tgggtgtatc aaatcgaacc
    11101 tacaaaatgt tctcggctga aagcgaagaa ggaggaaaca aatgaaaaag aaaattacta
    11161 ttactgcgat gagcctgtta acggctcttt ttttattacc cattaatacg tttgcttata
    11221 ctattaatga cgagtataat ttagcgccga atcaaggaga ctccagatta gcaattccta
    11281 acaaaattat tttgcacgaa actggaatag atgcaccagc aagaaacgta gccgccaaca
    11341 tgaaaaataa ttataacgga agcaattctt atactacaga tgttattggt gacggtggga
    11401 ttgtttaccg tgtgggtgag caaggatatg tttcgtgggg agctggtaac gctaatcctt
    11461 atgcgcctgt acagattgaa ttacagcgca catatgataa agcattgttt gaaaaaaact
    11521 atcgagctta tattgaatat acaagagata gtgcaaaaaa atatggaatt ccattgactc
    11581 ttgatcaagg aacttcttta tttacaaaag gaatcatttc tcatttgtgg gtgacaaatt
    11641 atgtttgggg gaaccacaca gatccatatg gttacttatc gcaaatggga gttagcaaag
    11701 aaaagcttgc
//
LOCUS       DACDIH010000078        11511 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDIH010000078 DACDIH010000000
VERSION     DACDIH010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.078"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1030)
                     /locus_tag="IV281_002284"
     CDS             complement(<1..1030)
                     /locus_tag="IV281_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8781255.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLRDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="IV281_002285"
     CDS             complement(1112..1801)
                     /locus_tag="IV281_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8781256.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IV281_002286"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IV281_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP8781257.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="IV281_002287"
     CDS             complement(2328..2831)
                     /locus_tag="IV281_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP8781258.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IV281_002288"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IV281_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP8781259.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IV281_002289"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IV281_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP8781260.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="IV281_002290"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="IV281_002290"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP8781261.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="IV281_002291"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="IV281_002291"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP8781262.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="IV281_002292"
     CDS             complement(6443..7462)
                     /locus_tag="IV281_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8781263.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8522)
                     /locus_tag="IV281_002293"
     CDS             complement(7635..8522)
                     /locus_tag="IV281_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8781264.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IV281_002294"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IV281_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP8781265.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IV281_002295"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IV281_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP8781266.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IV281_002296"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IV281_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8781267.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="IV281_002297"
     CDS             9753..10397
                     /locus_tag="IV281_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8781268.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="IV281_002298"
     CDS             complement(10453..10956)
                     /locus_tag="IV281_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8781269.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT         3363 a         2384 c         2099 g         3665 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtccct taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg a
//
LOCUS       DACDIH010000079        11360 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDIH010000079 DACDIH010000000
VERSION     DACDIH010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.079"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..725
                     /locus_tag="IV281_002299"
     CDS             <1..725
                     /locus_tag="IV281_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781270.1"
                     /translation="FGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGKKA
                     SDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKIIES
                     AKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIKTV
                     KLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVHVV
                     YEDGKVFGDSTANEALTIKLAK"
     gene            798..989
                     /locus_tag="IV281_002300"
     CDS             798..989
                     /locus_tag="IV281_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781271.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            1154..1741
                     /locus_tag="IV281_002301"
     CDS             1154..1741
                     /locus_tag="IV281_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781272.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            1741..2640
                     /locus_tag="IV281_002302"
     CDS             1741..2640
                     /locus_tag="IV281_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAP8781273.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            2640..3554
                     /locus_tag="IV281_002303"
     CDS             2640..3554
                     /locus_tag="IV281_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781274.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            3606..3821
                     /locus_tag="IV281_002304"
     CDS             3606..3821
                     /locus_tag="IV281_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781275.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKKFWLNNFSYKDFIKEKANTLY"
     gene            4010..4264
                     /locus_tag="IV281_002305"
     CDS             4010..4264
                     /locus_tag="IV281_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HAP8781276.1"
                     /translation="MKKEELIDIFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLNSDDVNFAHDVVGIQNHIDRENKRMGDEFLPRYSSL"
     gene            4443..5486
                     /locus_tag="IV281_002306"
     CDS             4443..5486
                     /locus_tag="IV281_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HAP8781277.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            5659..5883
                     /locus_tag="IV281_002307"
     CDS             5659..5883
                     /locus_tag="IV281_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781278.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            5886..6143
                     /locus_tag="IV281_002308"
     CDS             5886..6143
                     /locus_tag="IV281_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781279.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGLRSYTTSEKDK"
     gene            6245..6598
                     /locus_tag="IV281_002309"
     CDS             6245..6598
                     /locus_tag="IV281_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781280.1"
                     /translation="MISENLSYENNKHLVKENKKVEDYKNMYIEMKKSPLCIQYKNDN
                     WKQVVEFVKPHSIRRVRRGKKKSELVNSGRAVIRVITKDYPVEYGDYILRLSYHAFVV
                     LKEEHFNEFFNLKSK"
     gene            6651..7445
                     /locus_tag="IV281_002310"
     CDS             6651..7445
                     /locus_tag="IV281_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781281.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSKRLRITVEYGTNESATELKQRYLDTRREASKKYNDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            7474..8592
                     /locus_tag="IV281_002311"
     CDS             7474..8592
                     /locus_tag="IV281_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781282.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGNSKDEVKGVVIDDDFEFDFD"
     gene            8625..8945
                     /locus_tag="IV281_002312"
     CDS             8625..8945
                     /locus_tag="IV281_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781283.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            9200..10093
                     /locus_tag="IV281_002313"
     CDS             9200..10093
                     /locus_tag="IV281_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781284.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EDEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHEELSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNAYI
                     ARLKRFIDKYKENSDKFSKDK"
     gene            10113..>11360
                     /locus_tag="IV281_002314"
     CDS             10113..>11360
                     /locus_tag="IV281_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781285.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKEFEQLRFEN"
BASE COUNT         4385 a         1417 c         2393 g         3165 t
ORIGIN      
        1 aatttggtat atcgggagtt aataatagtt atgctccaga ttctttggga agggtagtaa
       61 tagatggaac atttgaaaat gtaacgagtt ataaaaatgt ttcgctgaca gtgaaggaag
      121 atggcaagaa agcatcagac gacgttctcg ctactattaa gccgtctcaa tcacaatttg
      181 aagtgtttta tacagttaaa gagaatagcc ctgtacacaa gctgacattg gtttttaacg
      241 ttgatggaga agaatactat aaagatgtaa aaataaataa aacatcaacg aaaaagatta
      301 tcgaatcagc gaaaaaagaa gtagctacag aagaaagtac aactgaatca agttctactg
      361 tcgaagcgga actaccagat aaaattgatt ttgatcctga aaaagtcaaa tcgtttgaat
      421 ctgtttctgg ttctatggct gatcggttta acactgaagt cttcaaagct atcaaaactg
      481 ttaaattgga tactgaaaag caaatgatta atgttacctt attcaattca gttgaatcta
      541 tggatcagga taaaaaagtt acggttgcaa gagaactagg aaaaagagta attggtttgt
      601 atcgtggtac tgctaaacca gattcagacg gtgcagattt gccagttcat gtagtttatg
      661 aagacggtaa agtttttgga gattctacag caaacgaagc attaacgatt aaattggcta
      721 aatagccaga aagttataat aaacaaatgg tgtcacacca ataatatata ggtgtgacac
      781 cttggagatg atagctgatg gtagtaaata aagaaaaagt tagaaagtat gtgactttaa
      841 aaatagcttc tgttaaatat ttagaaaagc gagctaaaga agaagaaaga gcaagaggta
      901 agaaagtaac agtttcagag ttgttagaag aattgatcga gaatgatcaa acaataagac
      961 gtgcatttac taataggtct atcagataac aatataatta ataaaaaaag aaagagtgat
     1021 ttggtcttag actaaatcac tctttttata taaagtttct ttacactcat tccgaataat
     1081 gttaaaattc tttatacgat atatagttcc ttgtcagagg aagagataac cagcaatatt
     1141 ggagggataa taagtggaaa acaataatga tattagacat tttagcattg gggaaagagt
     1201 tacaacagta agtgaattat caaaggatta tagagataaa gagggaacga ttatttctct
     1261 taataagaat cgaagaggcg tttggcaata tgttgttgat ttaggtgatg gcgttacagt
     1321 tgagtgtaaa gatagtcaac tgatcagaaa taatggcagt cattccattg aaaaagcaat
     1381 tcttgtatac ccaaaagtat caagtaagat catgaatgac tggttgcttg gaaaatttgg
     1441 tgagtacaat aaaaaagaaa tcgcagaagt gatcggcaaa tcttatccaa cagtattagg
     1501 gtatctaaaa aagataacga gtaaagaagc aaaaattgag gatgttttaa cagtaagaga
     1561 gcttcaatca ttaatcgagt atcggaataa acaaataaca aatcctgagt accttagaaa
     1621 agaagatggt ctattgaaac aattggaaca agtaaataaa atccggaaga tgcaaaaggt
     1681 agctccttta agccttgata cctttaaaaa aatctatttg aaagagatca aaaaagacta
     1741 atgtggactg taactttacc gttccaacct gtatctgcac caagaccaag tgcaagaagg
     1801 aatgctgctg atgaaactaa tgacgtagca gagaaaagga tttcaaccta ctatgataaa
     1861 aagtatcttg attatttgaa aaaaataaag agctttatcg aagatcaaga tttattagat
     1921 gatgaatttt actcgattgt aaatgatcct atgggcgcaa ttatggaagt ggacttttac
     1981 taccagatac caaaaagcta taaaaaagtc tataacatta tgaagacaac agcgccagat
     2041 ttagataatt tagtaaaaag tgctatggat ggtattttta acatctcagc tatacgagat
     2101 agccatatca caggcttaat tgcttttaaa tataatgtgg cgaacgaagg aaaaacagtt
     2161 gtgaggatca aacgatattc agaatttgag tgggatactt ctgaaggatt gaacggagat
     2221 atatgggaag ctacatttga ttttaaacca gtagcaacgc caagaccaaa gtctaaattt
     2281 agaggaaatg ggattatcac ttactatcca aaagagtaca acgactattt ggaaaatatc
     2341 aagaatacgc ttaaagagaa agatttggta aatgatcaac tgaaaaaggt aatgaatgct
     2401 cctatggggg ttatagctca gatagattat ttttaccaag tgagaaaaga taagaaaaag
     2461 cttgattcac tgatgcgtac ttcacagccg gatattgata atttagtcaa aggaacattg
     2521 gacggaattt ttaacaagga aataggcatc aaagatagca gggttgtagg gttaattgct
     2581 tttaaataca atacttttga aaagtcgaaa acaaacgtga gactacagga gatgggctga
     2641 tgaataataa tgaaaaaatt cttcatgtac ttgatagctt tgaaataatt caagaagaac
     2701 taaagaaata tcgggatgtt ttggaacaaa gatatgattt tgtcaatcag caaaagagta
     2761 atcacatgga tttcattctc aatatgaatg acttgaagaa gaaattagtt gaacgaaaag
     2821 aacaagaaaa gttgatcaaa gcttactttg aactcggtga aaaagaagtt aaaaatgcaa
     2881 tggaacttaa tgaagagcgc agggttttag atcagctgtt agaacaatta ttggtgatgt
     2941 ttcaaaaagg acgaatagat gaggatttaa ttgaagaagg attgagaaaa tatccttcaa
     3001 actcaggtat cggaatagtg ctgaaagcga ttgatgaaga agaaattgaa gctttcatac
     3061 cgccagagga ttttgaatcg gcaatggaat atataaaata ttatagccaa gggatcactg
     3121 cttttagaga gtttgatcct gaagatgtta ttcatgactt gaacaaccta aaagaatggt
     3181 gtgaaagtta tggagtcgat gattccggat tggactactt gatcagtatt atggaaatcg
     3241 aagaagagat gccggataag cctgatccaa cagatatttt agaattgatc catgaagcta
     3301 gaaatccaat tgcctatatt tctagaggat atactgtatt ggaatactat aagccttata
     3361 tatcagcgat gaaccattta agacgtgtat taagggaaaa acgtgaatac cgctcagtgt
     3421 tgaatgcaac gaatagatta gaaaaagctg tttcagagct agatgcttat tatcgtgaac
     3481 attacctgca agcaggtggt atgccaagaa atacgaaagc aaatatcagt aggtatataa
     3541 aaaaagctga gtaggcggag ttagcccata aaatggcaaa aggtaacctg aaaggggcat
     3601 tttgaatgaa aagggatgtt tttgaattgt acgtattaga acgtctagaa gacggcatga
     3661 tggcacaaga gataattgat gaaattgatg atatgatcag cagatttggt caattggagt
     3721 tgaatgatgg aaacaaaaat tcggtagtgc taaaaaaatt ttggttgaac aatttttcgt
     3781 ataaggattt tatcaaagaa aaggcaaata cattgtactg actaaattca cgaggagcag
     3841 tgatgagagc ttattgctaa gagcagttag gctctttttt gtttgtgtaa aattatttgt
     3901 tttataatac acatttatac acaaaaaata ttgacggata gtacacaaat gtggtaggat
     3961 atatttgtaa gcaagttaga ttaacaagtt aaaggaagga agagcagtta tgaaaaaaga
     4021 agaactaata gatatatttc aaattgttga acgagcaaat aatatgggaa ttatgttttt
     4081 tgatcgtatt tctttaaaga tggatttaag cgttgcccac caagaattta atctgcgatt
     4141 aaaagccttg ttgaattctg atgatgttaa tttcgctcat gatgttgtag gtatacagaa
     4201 ccatatagat agagaaaaca aacgtatggg tgatgaattc ttaccaagat attcaagttt
     4261 atgatttggc gaaagattca agagtatgtg tcattattga taggtactct tgaattggaa
     4321 aggaggaatg aatttaagac aacaacgatt atgatcttaa aacaatcgtt tatgtaattg
     4381 gcaatcttac gaataatttt catgcaataa tgacaataaa aaatgaaaag gggtaaaatc
     4441 aaatgaaaaa atttttagta ggaattctat tcacaatggg gctagtatta ttatcacctt
     4501 cagtttcagc agaaagctct atttccatgt atcgtatgta taatcccaat agcggagagc
     4561 atctatacac tagaagcgtt ggagaaagag acaatctgaa acgttatgga tggcattacg
     4621 aaggtgtcgc atggcaagct ccaacaagtg gtactcctgt ttatcgtttg tataatcggt
     4681 ataacggtga acatttttac acagttgatg ccggagaaag agataagatt tcacacacag
     4741 gatggacgta cgaaggtgtt gcgttctatt cctacaacgg agcaaataga gttcctgtaa
     4801 cacgtttata taacaaatac gtgaattggc atcactacac gttagatcaa aatgaaaaga
     4861 acgtaattag taaacaaggt tggaagattg aaggtacagg ctggtatgca atgccaaaca
     4921 gtgtaccaaa tcctacacct gctccaagca ctgaaaaagt gttgaacgtt cctgttgtat
     4981 ggcaaggaaa tacaatgtta tgcgaaggag cttctttatt agaaggttta cactacaaag
     5041 gtgtagccac aaatcaaagc ttaaccagtt ttgtaaacta tatgccaaaa gctaatgaca
     5101 ataatcctta tcatggttac tcaggagagt ggcgtcataa cgttaatgga acgtatcaag
     5161 gcatgatggc tgacccagta gttaaatggg caaaaaacgt tggcggtaat gccgtcaata
     5221 tcactggttc aggaacgaat ggaatcaaga atgaaattcg caatggtaat ccggttgtaa
     5281 cttggattac ttataactat gctacgccag agttcaaaca aatgccttgg ggtcgtgcag
     5341 tatggaatgg acatgtagtt ctcgtggatg gcttcagaaa tggtgcatac catgttgttg
     5401 accctgtatt tggcgcaaaa tggatcaatg caagcacgta tgagagagca tttaatgtga
     5461 ctggaatggc ggttgctgta cgttaattga tagaattatc aactaaatga aataagagct
     5521 taattactga aatggtaaat aggctcttat ttttttagag gaaatttttg taatacgaat
     5581 ctataacaaa aaatattgaa tgtagtacac aaaagtggta tattaaaatc gtagagatta
     5641 tgcgaagagg tgattttgat gaaccaaaga agaagaaaaa ggttagaaga aataagcgag
     5701 cgactttccg agctaaagga tgagttagaa gtaattcaag aagaagagta caaagaaaat
     5761 atacctaaaa atctacaggg atcagaaaga tatgaaaaag cagaagaatc cgttgatcaa
     5821 cttgaagaag ctgttgatgg tttatccgac atattagaat tgattgaaga tatagctagc
     5881 tagagatgat taatgaagat ttcaggagtg agaataaaat gattgatatt tcactgatag
     5941 agaagttact tttctcaaat aagtacaccg caaaagagct ggctacaaga tggcaaatat
     6001 ccgaatcagc tattaaagcc tatcgtcaaa ataagggaag cgtaggatgg cgtgattgga
     6061 aagggatcag cattggaaaa gcagaagaca taatgaaacg tgaaggactt cgatcctata
     6121 ccacttctga aaaagacaaa taacttaatg tttgaaaaga taaaatatct gtcttaaagc
     6181 tatgtcaaat tcaaatggtg aatgtttgct aaatataatg caaaaaaagg aagggaaata
     6241 ttaaatgata agcgaaaatt tgtcttatga gaataataaa catctagtta aagaaaataa
     6301 gaaagtagaa gattataaga acatgtatat agaaatgaaa aaatcaccac tgtgtattca
     6361 atataaaaat gataattgga agcaagtagt cgaatttgta aaacctcata gtatacgaag
     6421 agtacgaagg gggaagaaaa aaagtgaact tgtaaatagc ggaagagcgg taattagagt
     6481 tataaccaaa gattatccag ttgaatatgg agattatatt ttaaggttaa gttatcatgc
     6541 ttttgttgtg ttgaaagaag agcatttcaa cgagtttttc aatttaaaaa gtaagtaatc
     6601 atattttttt gtagacgtgt ataaagttaa ctaaattgcg aggtgatgac atggtagcaa
     6661 aaaagaatac tctaaaagaa gcttctgaaa aatatcaaga tatgaaaaga cgaggattaa
     6721 taccgaaaga caaaaaaggt atatggagag aatttgttaa ggaagtcagt agtgatcgaa
     6781 gtggcggtga ctttgttgga cgatatacag caaaaggcta tagcaaacga ttaaggatca
     6841 ctgttgaata tggaacgaat gaatcagcta cagagctgaa gcaaaggtac ttagatacac
     6901 gaagggaagc ttcaaaaaaa tataatgatt tcaagagcag aggtctgatt cctaaaaaga
     6961 aaaagggaat ttggcgtgag tttttcaagg aagaagatag caatcggaat aagggagact
     7021 tcattggacg atacacagca aaaggctaca ctaagaattt agggatcaag gttgaatacg
     7081 gaactggtga aagctcatgg gaattgagaa atcgctattt aaatacattg ttagagaaat
     7141 ataaagcaga attattggag ttgttcgatg ttaatgattt tatcgagatg gctgaaaagt
     7201 atggtcatga agaaggtttt atgatcaatg acattatggt tattgataat tttacggatt
     7261 tattagcact tgcgaaaaaa gcaagggatt taagagaaac gcagatgtat ctattcagaa
     7321 ataaaaacgg aagaaaccgg agaactgaat cgagtgagat tgaagattac tacgatgcct
     7381 ttacacacga tctgggagcc tttgtagacg agataagtgg tgtaatgaac aaatataggg
     7441 tctagaataa aaaagccgga gatgagcaga taaatgggaa acaatagcat atacacacct
     7501 gaaatgcaga ataaaattaa aagacttgtc ggattagggt ggatgacaag aggaaaatta
     7561 gattctttta gtacgatcaa atatcttttg atggacattt accgtcagaa caagcaaaca
     7621 ggaatacatt ttttgggtta tgataagccg gaagatgcac ctttctttgt tccaagttca
     7681 gcctatagag ccagtacctc cggtagcatg tataacttgg atttgtttga ggaaggagca
     7741 aagatgatcg aatatgattt caacgaagcc tattcgagga tcatgagaaa ttatcctttg
     7801 ccgtcaaaca cttatttgaa agcaaaatat aatacagatc aagctatttt gagagcgata
     7861 aatagaccaa aagtaagacc gtatgaaaat ttaaaaacgt atatgttctt tttggttaat
     7921 tttgaaggaa cagccaaaga aggaacatat gctaatccaa aatcacagtc gatgtttgcg
     7981 gattatggcg aaaatcccaa aacagctaat gagcctattt ggttatcgga gattgagcta
     8041 acacttatcc ttgattatta tgatattaaa gagatggaag tcctagatag ccacacattt
     8101 ttgacgaaaa aaggattact caaagaatat tttgaaagaa tcgagccatt aaaaactgat
     8161 cctgatacgt tgaagcttta taaaaatctt agaacgaaag tatttggtgc tatcggacaa
     8221 aaatcattaa gagaaggaga aacaagcacc ttcgataaat acagtatgta taatcgagcg
     8281 tattcctcca tggtagcagg cgtatttaga gatcgtatgg ttagatatga acagaaatac
     8341 gttcatagcg catatgggct attaaaaatt cggactgatg gattgtattt cagatccgaa
     8401 gtaccagagt ttgaagagct gtataaactt ggagtagtta agaaaaaagt tcatacaata
     8461 tccaaagaag aagtcgaaaa agctttagaa tttaaaaaca aaggatttga aaataagggc
     8521 tcctttggga actctaaaga tgaagttaag ggtgttgtga tagacgatga tttcgagttt
     8581 gattttgact aaatactctt tggaaaagat ggtaggaggt aaatatgaag aagaaaattg
     8641 tggaaggaaa tgtaggtgtt attattctaa aagattcaac tgactatgct aaatcaatgg
     8701 cactaaaaga cgaaaaagtt gcacgcatgg ataaagattt agttgaattt ctgatggatg
     8761 atacagaaag attaaaaggg gatcagtttt tgagtttaat gaaagacaaa ggcttaaaca
     8821 catatggttt atttgattat aagaaattag ctatagagtg ggtaaaagaa ggagaaatat
     8881 tttttattga agaatgggaa ggtactgaga acttagttct tcatcagtat cttttgaaag
     8941 cttaatctaa gtaaaacagt tctgactagt tatgcttgtc agaatttcaa aggatgtttt
     9001 ccataaccat catcattcaa aaataaagga tttgaaaata agggttcctt tgggaactct
     9061 aaagatgaag ttaagggtgt tgtgatagac gatgatttca agtttgattt tgactagatt
     9121 tttttgaaag gtttaagagt aaacaaggta agaactaaga tcatgaagat tgagagtaaa
     9181 gaagatagag gacgaatgta tgagaacaca ctatatttat ttagaagata aaattgtaga
     9241 tgaaattccg gcaaaaagtc gagaacaagc tagaaaatta tttattgaaa aatacaaaga
     9301 gatcaatgga gatagtgaag ttccaaaagt ggtcgtaaca gttagaaaaa ggcaaacaaa
     9361 aaagagaagt acgtttcatg tcagccctga tatatataat cttatagagc gacatagaag
     9421 tgaagataat atttctatct ctcaatatat tgctacagtt attgatgaat tgatttcagg
     9481 aaagtatgac tatgcttttg attttgagga tgaaaaggat tacaaaagtg ttgctgtctt
     9541 tcaatctgat tttgagaagc taaaaaacta tgctggcagg agaaatgtta attttgcaag
     9601 actactagaa gagttctact ccgaagagtt gggagtagga aatgaataca agagagaaac
     9661 ttatgcgaaa ttgtctcata agttatccca agcgcttaaa gaagtgacag aagttaaagc
     9721 ggaactagat gctaaacttg aagaagtgaa aaaatatcaa acagagagga agaacaacat
     9781 gaagaatcaa gtggaatttg acgaacagaa agaatttgaa aaagcaagaa aacgctataa
     9841 caaaattatg aatgaactta gctatgaaca cttaacagtt catgaagaat tgagtgaggg
     9901 tacagaaaaa tatacgatcg cagatatggt taatgaagtg gaaactcttt tagcttcttt
     9961 ctttgaaaat ggtcacgtaa acgatgaatg gcgaaaagaa gaacctaaaa gggttaacgc
    10021 ttatattgct cgattaaaaa ggtttattga taaatataag gagaatagtg acaaattttc
    10081 taaagataaa taaggagaga gtgtgattac atttgccaga aatcgaagga atattttcgc
    10141 caaaaatagg taaaaaacag ttagatttca gcagttttcc gagcgtaaat agagattata
    10201 atctaggtga tgaggtacag atactaaata ttgatttcaa agaactagac ttcgctgttg
    10261 ctgtttccaa caaacccaag gcattggaac gagtgaaaaa aatataccaa gagaatgatc
    10321 aaaatatcaa aaatatgatc aaaaagtata gacgagataa ttcaaaagtt gaagacgttt
    10381 tattcaacgg tacggcaaaa catgatttga atgtttatat ggcattagga gtggctttgt
    10441 actgtaattc aattaatcag acagatattt tggttaatat cgtgaggaat agctatcaaa
    10501 aaatattaaa aataagtagc gaaacaaacc cttattatgc aatgaaatat cttggggata
    10561 atttattaaa tgataaaaaa ttgatgcaca acactgaaga gctgttatct aatttggctg
    10621 cgattaactt tctattcaaa acaggttctg aagattattt tatagataac gtactaaaag
    10681 gtgcttacgg aacagcattt tatccgcttt acaatatgaa acagcataaa gccgaacaaa
    10741 aggataacaa acttgaaatt aaaaagatga aagaactatt aggtatagga tttgtaaagc
    10801 ccaattcaaa agtcactatt gcttcgttag caactactaa caatgatggg aaaatagtaa
    10861 atgggtttga cgattcagat atagatatat tgtcaaaatc atatttttct ttgtatggag
    10921 attttgcaga cttgatgggt attgatagtt acttagtcaa taattttgag atcaagggta
    10981 gcgagctgaa tgatattgtg tatttcgttt taatctctct atatgcaatt gatcagaata
    11041 ccgagtaccg acaaattgac tatttgctag ttttttatag tttgttatat cagttcgttt
    11101 taactcggtt gtttaaaaat gctaaggaag aactgatcct gaattctgat tatgaaatag
    11161 caaaacaaat tgcaagtatt agaaaagaaa acaaatctga agtaatcaaa ttaagaaaag
    11221 aattaagtat tgcgagtaaa gaaaacggtc ggttagaatc tttagtcaaa tcggcaaaaa
    11281 aagaagagga acttttgaag caagagatta aagaattaaa aggaatcaga aaagaatttg
    11341 aacagctgag gtttgaaaat
//
LOCUS       DACDIH010000080        11352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDIH010000080 DACDIH010000000
VERSION     DACDIH010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.080"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            155..361
                     /locus_tag="IV281_002315"
     CDS             155..361
                     /locus_tag="IV281_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP8781286.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(563..1564)
                     /locus_tag="IV281_002316"
     CDS             complement(563..1564)
                     /locus_tag="IV281_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8781287.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1569..3482)
                     /locus_tag="IV281_002317"
     CDS             complement(1569..3482)
                     /locus_tag="IV281_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8781288.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3650..4156)
                     /locus_tag="IV281_002318"
     CDS             complement(3650..4156)
                     /locus_tag="IV281_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8781289.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4316..4756
                     /locus_tag="IV281_002319"
     CDS             4316..4756
                     /locus_tag="IV281_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8781290.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4782..5939)
                     /locus_tag="IV281_002320"
     CDS             complement(4782..5939)
                     /locus_tag="IV281_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781291.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(5942..6298)
                     /locus_tag="IV281_002321"
     CDS             complement(5942..6298)
                     /locus_tag="IV281_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781292.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6596..7570)
                     /gene="sgrA"
                     /locus_tag="IV281_002322"
     CDS             complement(6596..7570)
                     /gene="sgrA"
                     /locus_tag="IV281_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP8781293.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7766..8605)
                     /locus_tag="IV281_002323"
     CDS             complement(7766..8605)
                     /locus_tag="IV281_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8781294.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8790..9677
                     /locus_tag="IV281_002324"
     CDS             8790..9677
                     /locus_tag="IV281_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP8781295.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10271..10966)
                     /locus_tag="IV281_002325"
     CDS             complement(10271..10966)
                     /locus_tag="IV281_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP8781296.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(10950..11348)
                     /locus_tag="IV281_002326"
     CDS             complement(10950..11348)
                     /locus_tag="IV281_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8781297.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3386 a         2279 c         1697 g         3990 t
ORIGIN      
        1 acctatctga ttgctgaatc attaattgct tatcattagc catctttgtt tcaagttcgc
       61 tagatgatca ttttattcac atcctaacaa acttgtcaca tttatagatt cttaatctat
      121 ttatcataca attataaaaa aggaggtgct gcttatgaaa gctatccata tttctttgct
      181 ttttttcgct agtgcattga ttttatttgc tattcatctt tgttcccagc cttatatcaa
      241 taacaacgga atattagtgg aaccagcatt cttttgctta ccactcggct ttttgtccct
      301 tcttgcctcc gcaggcttcg gatttgttgc tttttttaaa cgatcaaaac aacaaatctg
      361 acttataaaa tttacgtaaa agagatcgtg acaataatcg tgtgatattc ccaaatttcg
      421 aataaaaggt gccccttgcc atctctaact cctcagtcac aagttacaat attcataaac
      481 catgaaaagc ttgtttattg aaattgctcc ttgtgacttg gagctaagtg acagattaca
      541 acctcttttg catcataaga ctttactcct tgaaaaacat tactaacttt tgatatgttt
      601 cttgcgcttg tttattgctg aaatcaaact gatattcatg gttcaaaaca gcatttttct
      661 ccgcataatc tacttttgtg actttcaccc ccgctttctc aaatacagaa gttacttctt
      721 ccatttgttt cgtaaacgta tttgtatttc catccgttaa gaatgtgttt tgcgtccaac
      781 gatccgcatg tctgaccaag gctaaacttt tgaccttctc tgattgttca aagtccttat
      841 tatcaaagta agcccatcca catttttcat aaagaaagct agagatttta tcacttactt
      901 gagtaagtgc agaaggatcc aacagaccag acaaagaaat aaacttcgta atctgcttgt
      961 tctccaaagt cggatcaaga tgatcagctt ttttaatttc ttcattttga agtgctaaca
     1021 aatattctcc actaatttgt gcacctgccg aatttccacc aatcgaaata ttcgaccaat
     1081 ctgcttgatc ttttatctct tttttagctg cttgaatggc ttcgttcaat tggataactg
     1141 gtgtaggatg ttttgcttcc ggtgatcgtt cataatctat atttacgatg atttgctgcg
     1201 tgtctgaaca tagatattcc aaataatcac gacaatcatt tttatcccct cctacatatg
     1261 cgcctccatg catccagaaa agaatcggta attttttctt ttccactttc ggtgtgtaga
     1321 tatccatatt attatttttg tactttgaag gataggatat ttcttttttt atatcgattt
     1381 gttcgatttt ttgctggaaa tcatctggtc gggcatatac ttgttctttt tcacttgtgt
     1441 caaattgttt tctaacaagc aagctaatcg gctttggtgt gatttgatta atcaaaaata
     1501 cagtgactaa aataactacg atgataactg gtactgcaat cactattcgc ttgaaccatt
     1561 tcattcattt accctctctt tcttcgccaa atccatgtga taatcgtgct aacagataaa
     1621 aatccacctg aataaatgag aagcgaatgt ttcttttctc cagttttcgg caacattctt
     1681 ttttttgcat ctgtcttttt ttgtactttc actgtcatct gaatcggttg cgtatcatca
     1741 cttgattgat cctctcgagg ttcattcttt cccttattgt ctgcattctc tgtcggatct
     1801 tgcttttttg gtggagctac tgggattttt ttgtaataga aaataatagt tggtatcgtc
     1861 tcattaaaaa tcccttcagt cggtccttca acatttgcta attcgtatcc ttcgaatgtc
     1921 ttttgctctg tcatataatt ttctccataa gctccgtaaa gctggatttc gtcctggtct
     1981 ttgagtagac ttttattgtt ttcatccaca tactttacgt ttatctgatc atggaacact
     2041 actttcaaaa agtccgttac atgtttcgtg taggcagcat ggtcaacttc ctgtgccttt
     2101 ccatgccctg ctccttctac taataaaaga tctttatacc ctcctgataa ctcatataat
     2161 ttttgcgcgt tagacacagg tactacatca tctgctgttc catggataat caatttaggc
     2221 aatgaatctg acattccaat cgtaggtaga gcagcttttg tatccaaatg catctcctga
     2281 tcaaaatagt attgtttcaa aaggcccata aataaatctt catgctcttg atccccaatg
     2341 atgtctaact gattttttat ggcatccggt agtgcggggg ctactgtctg gctgtacgta
     2401 aaacgtagtt gttcatcaat tgatgcaaat ccgcaatttt caataattcc tttgacattg
     2461 acaggaatat ctttcgctaa tacagacatc atcgtagctg ctcccatcga accaccataa
     2521 agtaaaatct tttgagatgg ccgctctcga acttcttgat ttatccagta aaggacgtca
     2581 tctgactcat attgtccata ggtcacataa tcgcctccgc tattaccagt cgctctggaa
     2641 tcaacaaata ataaattata cccggcctta taaaaatcat tgtattcttc tgtaacgatc
     2701 ccgttattcc aattccctcg aaatccgcca tggatcatga ctgttttatc tgaaccttga
     2761 tcgacataat atgcatatac atccatcatt ttgccatctt gtttgtttag tacagtttga
     2821 tgaagttgtt ttgtatttgg atagaagcct gtgttttctg taggcgttgt cccatcatct
     2881 gtacctccaa gcatcgacat caacacactg ttttttgcaa aagcgatatc gtacaataga
     2941 taagtagcaa tcagatgttt gataatctct ttatctacac cataatcatt aacagcaagc
     3001 tcagtgacta ataactctat taattttgac ttgatgtctt ctgttggtcc attaagtatt
     3061 tctgtcatct gtttaccgaa gtaattcaaa tattctttac cattgaaatt ttcaccttta
     3121 ccgatatcat cattttctgt cccattctct ttgtcactta ctttagcaga agaggattcg
     3181 tcgtttgaca tttttgtagc tgttgcttct tctgtttgtt ggttatctgt ggtagcggtc
     3241 gtttcttgat ctgtgctagt aacagatgtc tcaaccgttg tttcagtatt tgtttctgtt
     3301 tgtgaagtac tcgatttcgt ttcatttcca gcatcagtag tttctgtggt ttctacctcc
     3361 cgagattccg attcggaaga aatgctctct ccatatactg tccattgcgg gacgcttgta
     3421 cttacagcaa ataagatgac caacgctccc tttaatactt gttttgttct caagctattc
     3481 actccttttt atgtttctag gagaattata tgtaaacgca aacatttttt atacaaaaaa
     3541 taaatggttt atcccataaa aatagaacag gattattttc tcattttacg taataaagca
     3601 gaatggataa accagtatcc attctgcttt ttttaaacga agaaacaact tattgctcca
     3661 aaaatgtcag aaatcgttta tcccaaattt tctcgtaaaa agcgattgtt tcctttgctt
     3721 tcataaaagg attatcataa gttgtcgttg ctcctctaca catcataagc tggtatccta
     3781 atccatgagc aaacttcaca gtcgtatcta tacaatactc tgcttgtgcc ccacataatt
     3841 cgatcgaatg acagtccaat tcagttaata aaacactcaa attagtctta taaaatgaat
     3901 tcgcatgggt ttttctcaca aaataactgt gctcaggaat agataattcc gacaatagct
     3961 cccatggttt tgtttgtggc actaattgag catcttcgtg ttgaacgaaa ataactggtt
     4021 tcccaatcgt ttcgtatgtt ttgattcttt tattcactaa tgctgtcact gtttctaatt
     4081 cgtgtatcgg cgaagaatca aaacataccc ctttttgtaa atcgatcact actattgcgt
     4141 cggctgtgtt tgacatactt gtgccttctt tcttttttct tgatttaata gtatttctta
     4201 tctagctagt tccattatac gtgacaacat tttcttattt tacacctcta aatggcactt
     4261 ttcgactatt tcttcatgtt attatttcat aaatacttag ctattggagg aatgtatgat
     4321 gattaaattg tatacaggtt ctggctgctc gtcttcaaga cgcgcaaaac gttggcttat
     4381 cgtccatgat ataccgttta tcgaacagaa cttaattcgt caaccactaa ccaaaaatga
     4441 atttatccac ttactaaaat tgacaaacaa cggcgtgacc gaaatgctct ctactcattc
     4501 tattagttat aaaaatcttg ttcacagaat agatgaactc agtttgagtg aactatatat
     4561 tgagattcgt acatcccctt ctttattgaa aaaaccgatt atctacgacc aatctgcaaa
     4621 acttttgact ggatttagcg aagataaaat caggatgttc ctttctaaaa ctaaaagaaa
     4681 gcaagagctc aacgaactct tgctttctgg tcatcttgtc gattttaaag aaaataatct
     4741 ttttgcaatg tcttaatttt ggcataaaca atctgctttt cttaattaac gaaatccaat
     4801 ttttccaata tggatctatt tttctcattc ctgattttta tgatttcagc tttcagacgc
     4861 gcccaatcat tttttgtagg ttgtccattc cagtaaaata tctgtgtatt cgtttgatca
     4921 tatgcctttt tgtcgatagg ataatcgcta atgacaatat caggtaactg atcagcggat
     4981 gcactatatt ttattgggcg ttcagtcata gataatatcc ttttctttgc ccataattcc
     5041 tgtgttttac catactttga ttgaatatag aatatgacag gttgttcgtg ttttataaaa
     5101 atatccctga ctaacataca gtattgaaaa atcaaccgtc tgttcttttc ataataagta
     5161 ttaagctctg gaattctgcg agctacccca ctaaaaaact gttgcatgat tggatacatc
     5221 actggaaata tatgctgaaa atctctgtct tctgctgatt ttccaaaagc atccaccttc
     5281 ttttttgtcc caaaaaccct tgaatacaaa tggatcattg ccaaattgac atatagataa
     5341 tttttttctg aagttgtaaa acaaatcttg aagttttttt caaaattttc tacaatagac
     5401 gattcaatcc agccccaact ttcttgtact ttatctggag tatccaacaa atcttcttcc
     5461 aaataggtgt tcattacacc aaacataaaa taaaggtaag cttgctctgt tggagtgatg
     5521 tgattcatcg accagaagtt tgcttcaaaa aaaggctcta gtatgtaatg cgtaaactct
     5581 tcttttgtta tcaatggatg cacaaatttg ttcatctctt ctgggacata cgtaatcgag
     5641 tggccttgtt tcattcgttg caacgaaatt cttagtaggc tgatccattt ctttctatct
     5701 atactacgcg tatatggcgc atgtttcgca aatttttcta atagttgacg tttatttttt
     5761 atacttgata atatatcaga atgattttcg tcgaatgact gccaatacat caagtgaaaa
     5821 aagtatcgga tacggtattc ttctccttct atcggattac cttttttcaa gctgcaagat
     5881 aaatgatagt ttttcaaaat agtattaagt tctcttaatt ttctttgaat cgtacgcaaa
     5941 cttatataat tttttgacta agattttcta aggattgata agtacctttg taaagtgaat
     6001 ccagtaaaat ataaccaatc gttccctcta aaagtataga ataaacttta ctcagtaaaa
     6061 actgagaaga aaactcgact tttatcatct tgttttctga aagaaaagat aatttatatt
     6121 ctggatacct ttcgcaaaac gattctgctt gttgaatcac cgaattcatc ttagccaggg
     6181 tgtagtttag atctaaagcg atatccttca ccacatataa tttctcgtct ctttcataac
     6241 aatagttcaa aaattgatat acttgccttt cattgatatc aataaattct tcaaacaaag
     6301 tacatactcc tttttatcta ttatttagac ttattccgac agctacatga ataacgtaca
     6361 aatagattcg ttactgattt ttcagagata ctaccatgcg cagtgtgcat tgtcaattat
     6421 tcttgctctt attttccatt tgtgttcctg ttatttcctg tttctcaaat atgttagtaa
     6481 acaaaaatag atctggcaaa gctacaactt cgccaggcaa tacagcactc tcgaatgaaa
     6541 tattcactca aaaatctatc gttttattca attttagatc tgttgctttt attctttatt
     6601 tcactcgatg gaagagagaa cgaaaatagc ttgctcctac aatcaaaatg agtaacacac
     6661 ccatccatgt aattagcttg ttcaaggttc tactaccagt ttctggtaaa tcgctttttc
     6721 tattatctgt ttctttcgct ttttcttgat gatcaattcc acgagaaaca aaatcaggct
     6781 gttttagcac actttttagg ttgctccctg catttctctt ttgttcctta gttggtatga
     6841 taggtaccaa atcaagttca ttatttactt ttcctgtgct gctttcgctg ctcgtgcttc
     6901 ctgtgctact ctcactactc atgcttcctg tgctactttc actactcgta ctctccgtac
     6961 tgctttcgct gctcgtgctt cctgtgctac tttcactact cgtactctcc gtactgcttt
     7021 cgctgctcgt gttttctgtg ctactttcac tgctcgtact ctctgtactg ctttcaccgc
     7081 tcgtgttttc tgtactgctt tcactacttg tgctatccgt actgcttggc ggtccaacaa
     7141 ctgtccaact gcttggtttc aaattctccg tgatcgtcat ctgtccatca taaaaaacaa
     7201 tcaaatcaac tttatcttta ctcttgatct catcaatcga tagttctgca gaaatcatta
     7261 ttgtatactc aataccagcg gtccattgta cgtcttttaa ataaacttcc ccaataactt
     7321 tttctgaact tccattacca tcccaaccat atgttagaac agcatgtaca tcactagcat
     7381 tcacgctttt gtttggtttg actggaatag taatgacttg ttgtgtcatg tcataaacaa
     7441 tccgatcagt tttcacagta aatgaaagat tctcatttgc ccgttcattt gcagaagtac
     7501 tgattccaat cattgctgca attatactca gcccaagaat aacaaataat acggtcgctg
     7561 ttttcttcat ttctatcact ctccatataa ttaccttcta tttgataaag gaattatata
     7621 aaatgaaaat gatgagaaca cgacagtcag ttgtgaaaat ctaacaaaag atgtcgtgta
     7681 ttcgcttgaa tttgtttact ttttcgttta atcaatgaca gacttaaaaa agcaagaccg
     7741 atatcagcct tgctttcttc atcttttatt gataaacagc agtgtatttc ttcatccgct
     7801 ttgttttata tgcatagagt aacaaaccaa caatcaagac taacaagaat aatgtagttg
     7861 atatagatgc ttcagcccca aagtttcccc cagtaattat tgtattttgc gattggatca
     7921 gagtggagaa aaaaccttgt tcttgcgttc cgctgactgg tatacccaaa atagctcctt
     7981 gtgtccaatt ccaagtaata tgagcaccaa tagccgtaaa taaacttcct gtactgtaga
     8041 taagtgcgct gaaaaagcaa cctgctaaaa agatattgaa aactgccaga aatgtcatgt
     8101 taggattcaa tccgtgcaaa agagcaaata atgcagcaga tccaatcatt gctattaacg
     8161 gaccaaatct aagcatctga tgctgaaaaa agccacggaa taaaagctct tctgatgccc
     8221 ccactgcaat aaataaaata aaactcgtta tcagcggcac aatgtttgcg tttcctgaat
     8281 ctaaccgata cgcccccgtc aaatataatg gactagtaat aagcagaaac actccaattc
     8341 caacaacgag acctcccgct aaataccccc agtcttttcc tcgaacagaa acaccataat
     8401 cttctattct ttttccttcg accttactag caagaaataa agctagtaaa aaaacagcta
     8461 aatgtccgac cgtaccggca attgctccaa tccattcact atttccaata aacatatccg
     8521 caagtgcatt attgacaaga attcctaaaa caaaaccaac taagactatt aaaacagatc
     8581 caatcaaccg aataatcctg atcatctttc atccctcctt tatattaagt ttaacaaccc
     8641 ttttctttct tgtctcgtga aaactatgta aatgttctcc atcatagaga gaatagattt
     8701 ttacgaacta agaatatcta cagcaacctt acgaaaatga aagactgtct ccctaattct
     8761 tctgttagac tgtttttaat aaggaaagga tgggaaattt tttgaaaaaa caactactaa
     8821 ttatgtgcct ttcagcatgt gcaattttac tattctcagg ttgtagcaag tccaatgaaa
     8881 cgattgcaac gtttaaaggt ggaacactta cagttcagga tttttatgat aacccaaaag
     8941 ttaaatcaaa taatcaaaca ctattaaaaa aaatgattgt cttcaaagca tttgatgatg
     9001 tatacggaaa agagatttct tcaaaggaag taacaaaaga atataatgaa caaatcaaaa
     9061 aactcggtcc aaactacaag gaacaattaa aagcagttgg ccaaacagaa gagacatata
     9121 agaaactctt taaacagatg ctggctttcc aatacggttt gaaagccaat gtaaaattaa
     9181 cagataaaga tctagacact gcttggaaag aattctatcc agaagttagt acacagatca
     9241 ttctattttc gactaaagaa gaagcagata aagcaaaaaa agaagcaaat gaaggagaaa
     9301 atttctccaa gcttgttcaa gcttatggaa aaaataaaac attaaaggaa actgatggaa
     9361 aaatgaattt tgattcaacc aatccagaga ttcctactga agtaaaaaaa gcagcattta
     9421 aactaaaaaa cggggaagtt tctgatatca ttcctgtaac agatccaacg acttatcagc
     9481 aaagctatta tctagtaaaa atggtaaaaa aacaggataa aggttctaat aaagacaaat
     9541 acaaatctga gctagaaaaa attgctaccg aagcaaaaac tcttgatacg gaattcatgg
     9601 ataaaacaat ccgtaaagtg atgaaaaaag acaatgtaac tattaaagat ccttatgtaa
     9661 aaaatatatt caaataaaca aatttgaccc aattggggat gtggcaaaat ttgagaagtg
     9721 atttttggaa attccttctt atttttgagg caaaccactt ttgccacaac ttcatcgggg
     9781 ccttttttta tttgtaccaa aacaaatcac tggatttgtt acaatcatga ggatataatt
     9841 taaaaaagtt ttttatttct atcacatgaa tcaatgcgcc attaagtaga ttctttcatg
     9901 cttccctatt ttttcacttg ccgtatcgct tcaaaaacaa ctatcataaa ctcaaactcg
     9961 actgaaaagt tattttaaaa aagactaaaa atatcccttc tggacttgtt cttcttattc
    10021 tgttatacta gagaaacacg aaagatattt ttatgcgaca cttttcattt gtttttatga
    10081 aatttattca aaactggcgt aacttcagag ttacaaggat gtattagagg ttagggaata
    10141 catcgtttaa atgagaaata agtaatagtt agtaatagtc acccaaactt taaattaatg
    10201 aatattgaat cgtcagatag cctcctcgag ttttcgagaa ggctatcgtt tattttatgg
    10261 agaaattgat tcagaagtaa tcagcgacct tttttagttc accaatcgtt tctgcttgga
    10321 aagtttgcac ccaatcttct tggaagcccc tcactgcttt atcaatcgag ggcatctcta
    10381 atacttgatc tagtagctct ttacctaacg ttatcgcatg agctccagat cgaatggctg
    10441 tagtgacttg ttcacaattt ttgaaactag ctgctaatat ttttgttggt ctttgcgtac
    10501 gttcgatctc agtccgcaac gaatcaatca ctttcatagc atcaatgcct tgattttgca
    10561 tcctgttata ataaggtgca atataatctg ctccattatt aatagctaaa tacccttgta
    10621 attctgtgta aatagctgta ccggtaatca aataaccttc ttttttcaac tttttgatcg
    10681 cttgaaggcc tgcttgattc accggcacct tgataaacgt atcttttccc agttcttcta
    10741 tgataactcg agcatcctct atcatttctt cttccgttct tcctattgtc tgaatatgga
    10801 taggaatctg tccgattatt tttttgattt ctttcatatg cgagaagaag tttgacgctc
    10861 cttctttttt cagaattgta ggatttgttg ttacgcctgt taaattcaaa aactgttgac
    10921 aagaataaat actttttgta tttgctgtat caagcatgat ttccatatag ctttccccca
    10981 ttctgatcct cttgatcgat tttaatgatt gtcccctttc ttaaatgcgg catttcctta
    11041 tcttctacat acattgttcc tggaagttcc ggctcatgag atccatcaaa ttttatcgtg
    11101 atatgtccta attcattcaa attagtttgg acaacatctc ctactgctgt gattacatac
    11161 atttgtttat caaaacagag cttcatatca caagttattt cttttcttac ctgattcaat
    11221 tcaatcttat aacaataatc tgttaatgtt tccggtgctt catctccaaa taaaatcatt
    11281 attttttctt tcctgaatac ttctgcttct ataccaacat ctgttacaac cgtttcaaag
    11341 atcgtcatct ct
//
LOCUS       DACDIH010000081        11040 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDIH010000081 DACDIH010000000
VERSION     DACDIH010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.081"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            51..1412
                     /locus_tag="IV281_002327"
     CDS             51..1412
                     /locus_tag="IV281_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP8781298.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1434..1694
                     /locus_tag="IV281_002328"
     CDS             1434..1694
                     /locus_tag="IV281_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781299.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            1708..2460
                     /locus_tag="IV281_002329"
     CDS             1708..2460
                     /locus_tag="IV281_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP8781300.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2554..3159)
                     /locus_tag="IV281_002330"
     CDS             complement(2554..3159)
                     /locus_tag="IV281_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8781301.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3212..4186)
                     /locus_tag="IV281_002331"
     CDS             complement(3212..4186)
                     /locus_tag="IV281_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP8781302.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4564..5343)
                     /locus_tag="IV281_002332"
     CDS             complement(4564..5343)
                     /locus_tag="IV281_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8781303.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5410..6681)
                     /gene="celB"
                     /locus_tag="IV281_002333"
     CDS             complement(5410..6681)
                     /gene="celB"
                     /locus_tag="IV281_002333"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8781304.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            6834..8231
                     /locus_tag="IV281_002334"
     CDS             6834..8231
                     /locus_tag="IV281_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8781305.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDFFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8312..8752
                     /locus_tag="IV281_002335"
     CDS             8312..8752
                     /locus_tag="IV281_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8781306.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            8900..9226
                     /locus_tag="IV281_002336"
     CDS             8900..9226
                     /locus_tag="IV281_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8781307.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9227..9976
                     /locus_tag="IV281_002337"
     CDS             9227..9976
                     /locus_tag="IV281_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781308.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10056..10511)
                     /locus_tag="IV281_002338"
     CDS             complement(10056..10511)
                     /locus_tag="IV281_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8781309.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3492 a         1932 c         2242 g         3374 t
ORIGIN      
        1 gttggttcat agttttattt actatattta gaaagaaaga aggtttattt atggaagttt
       61 tccaaaccat tatcaattat gttttgaact taggatcagc catttttgtt cccctgatta
      121 ttttgatact tggattgttg gcagggatga aattaaaaaa agcattcatg tctgctttga
      181 ctttagggat tgcttttagc ggaatgtcta tggtcattgg ctatatgtct aacgcagtaa
      241 gtccagcatc agaagcattg gctaaaaata cgggaatcag tttgcctgca cttgatcttg
      301 ggtggacagg tgcagcaagc atcacatggt cgtggagtta tgcttttata ttttttgcag
      361 taacgattgg ggtcaatttt gtactgttac tttttaattg gacgaagacg ttaaatgttg
      421 atatgtggaa tgtttggggg aaagctttaa ctgcttattt agtttattat gtttccggta
      481 gtttggctgc aggctttctt actgcaatgg tccaagttat attggaatta aagcttggtg
      541 atatgtttca aaaacatata caagatctca cagggattcc attagtaaca gtgactcatt
      601 ttatgacatc tgccgctgtc ttgttgctgc ctttcaatat gatcatggat aaaattcctg
      661 ctttaaataa acgagcggat acgaatgcat tgaagaagaa aattgggatc ttctctgaaa
      721 actctgtaat gggattcatt attggattgg ggcttggctt tgctgctgcg tatggcgttt
      781 caggttctct taatctagcg atccaaacag ctacagcatt aagtttgttt cctatgatct
      841 ctaaaatgtt catgcaatct ttgtctcctc tagcagacgc tatgtcagaa tttatgaaaa
      901 agcggtttaa ggatcgtgaa gtatatatcg gattggactg gccgatatta gctggtcgtt
      961 cagaaatttg ggttactgct attttacttg ttcctgtgtt tattggttat gccattattt
     1021 tgccaggaaa ccaagtattg ccattagcag ggattatcaa ctattcgatt gcggtcggcg
     1081 gattactttt aacaggagga aacttggctc gtatgctgat cttgggaatc atcactatgc
     1141 ctatttattt gtacggggcc acttaccttg cgccaatact cagtgattta gcagaaaaaa
     1201 gcggagcagt cgagggaatc aagaaaggac agttgatcac atggtcatcc atagaagggc
     1261 cggagttcag ggttttattt gcggaggcat ttaataggaa tattctcgct attgtaggat
     1321 gcgtgatttt tctaggactg tttgttctac tttataaata tatgataaaa gcgaaagtac
     1381 cgagtgaacg ttatgctgaa ctacaaaaat agcattgaaa tggtgggaaa gtaatgaaaa
     1441 gagaagagac agataaaata aaatggacag tcgctctttg tggtacactt ctgctatttc
     1501 tatatggact ctttactcag aatatcatca tcaatctatt ggtcattttt ttcgcactag
     1561 tgatctataa atatggaaat cacgttttat tcagagagta cgatgaaaaa agaaaacgga
     1621 aaatagaaga atcaatgaaa ataaaagaag ctaccaaaga aatattgaga gaaaaaagtt
     1681 ttataaaaag ataatggagg ggtcattatg tcagatggac gtatgttatt tgagagagaa
     1741 cgacaggaca tggcacgtat cgtaagagtg attttcgaaa ggaaaaacac aaatgtcgca
     1801 ggagggaatt tttcctttaa aaccactgat gatcaaggga aagactacat catcatgacg
     1861 ccgaccatga tgtcagaagc ctatctagga gagatttctc ctgcacaggt tctagtagta
     1921 gaaccaagaa cgcgtagaat cgttgacgga gtcggtcatt taactcgtga aatcaatatg
     1981 catgaagcga tgtatgagac taatccagct atcaaagctg tcctacatgc tcatgcgcct
     2041 aatgcaatgt tctgggcgac tactgggttg cctatgccta atttgacaga agccacagct
     2101 aaagtaaaag agattcctgt catggacttt gagcctaatt gctctgaaga acttgctgaa
     2161 ttgacaagcg actatgtaaa aaaacagcat ctcgaattac cgcatatgtt gctgctcaac
     2221 tctcatggcg tattgatcaa cacaggtgga gaatcaggaa tcgaagctat ccataaagca
     2281 ctgcaaatcc ttgatacagt tgaatggaat gcagaaattg cctacaagca gacgattttt
     2341 caagcattag ggattactga cggtttttat tcaaaaggtg agaaaatcgg gggattggaa
     2401 gagttgaaac ataaaacggc tatttataat aaatctgtta ttgcagctgg tggagactaa
     2461 cctatctgac acaaaaaact acgactttca caaaatctga tccagatcga tatctgcttc
     2521 aggttgtatt atgtaagtcg tagttctttt cttttattta gttaagaggt ggtctgtcca
     2581 ttccctcgct tgcttgattt cgtccatcgt caactgatga cctgttacag ttagctgaat
     2641 agtcacattt gcttggcgtg tttcgaattg atcggccaat tctttaaagg aagcagggga
     2701 aacgattgga tcgttatcac cataagaaat ccagacggtt ttatcctcta atgtggaacg
     2761 gctttgatga acacctagag acatcggatg gaaaaacaac cctttattta gctcggtctc
     2821 acgctccaat aataaatgag cagcgatgtt tgcgccattt gaatagccga caagcagcca
     2881 gtcacttaac ggtatcagtt ctttttcgct gatttctttg attttttcat ggagtttatc
     2941 tgtttctttt tccaaactgt ccaggtcgaa ttgattcaat ccattacgct tgaaaaaacg
     3001 attcatccca tcttcttgga ccgtgccacg aaaagaaagc aatgtactgt ttggtgcaag
     3061 aaagtgagcg atatccagta aagaatgttc atcgccacct gtgccatgaa gtaagatcaa
     3121 atgcttgcca cttgttcctt tttcaaaaat gtaggtcatt ttgttccctc ttttctgttt
     3181 ctatagatgc aaatgaagta gcatgaagag attattcacg ttttttattt gcatcacttg
     3241 tatcgaaagg acggacataa gcttcaatgg cttcacgacg ttcttctaag aatggaggaa
     3301 gagatagttt ttctcctgct gattctgctg gttcatcttc aaggaatcca ggaccatcag
     3361 ttgctaattc aaacagtacg tgatgagcgg ctaggaagta ctcagattgg aaatagaaac
     3421 gatcgacgaa accagaattt ggcaattcaa tcttattgat tacttgaatc cagtattcca
     3481 atgcttcttt gtctgctaca cgcaatgcta agtgatggat atttccaaat ccttcgattg
     3541 cttttggcaa gtctgtccga tgttcaataa tgatgcttgc accatttccc ccttcaccga
     3601 tttcaaataa gtagaaatct cctgaatggt ctgtttcttt gaatcctaag attttttcaa
     3661 gaatcaagcg gatatgctct aattgactga cacggacaaa gacaggtcca agtcctgtga
     3721 tcgcaaactc tgtcggtaca ttgctgtttt tccaaggaat gccagctgct acaccttgat
     3781 tgttttcgtc agagatcaat tgataatact gattatcgaa atcttcaaat tcgatatatt
     3841 ttttgccgaa acgttcattg attgctgaat gagtaacgtc atatttttca aaacgttcca
     3901 accaaaagtc taatgctgcg tcacttggca cacggaaaga agcacgagag atcgtatctg
     3961 ttcctttttt tccttttggc tgatttggaa aatcaaagaa agtcatatct gttcctggcg
     4021 caccagtatc gtctgtaaag taaagatggt aagtttcgat gtcatcttgg ttaactgttt
     4081 ttttgattaa gcgcattcct aaaatatctg taaaaaagcg gtagattttt gtagcgctag
     4141 atgtcattgc tgtgacgtgg tgaattcctc tgatttgtgt atccatgata aatttcctcc
     4201 tgttaactta caaaaagtaa gtagttgatt tagacatagt atagagggtg catctctatt
     4261 ttgcaattaa tttgtcttct aaagtatcag gaaggaatca agaatggaaa agaggaggga
     4321 cggatcaata aaataaaaag acttacgact agctgtatcg atttcaaaaa aactagcttt
     4381 aaaaagctaa ctttagaatc attacgggtg gtcataagtc tttttatgct gagttatttt
     4441 ttcatttttg agctaatgct taggatagaa tagaatgttg atttcacgat ctcttcttgt
     4501 tacttgaaac tgcttaccac tgtcacgatc tcttggtaaa cggctttttc acaacctctt
     4561 tggttaagtt gaacgcacat ccagcacaga atagttccga acatcaaatc ggtttcggct
     4621 ggaagaatat tcaatcggct gtccatcttt gagatagacg acttgctgaa cttccagtac
     4681 aggatcgttt tctgcacagt tcaaatattg ctgatcatat tcatcacttt tatctgcaga
     4741 aatagcacga taagcccctg caaaatggac attcaattct tgatgaagat gatcatagat
     4801 agaggctaac aaatgttctt tttttaaacc cggcacaaga tggactggca taaaggtatg
     4861 ctctaaaaca tatggctcgc catctaagat ccttaaccgg tgaatattgt agacaggttg
     4921 ctcggcatcg atcatcaatc gttcttggat atattgatta gggaattcaa catcaaaatg
     4981 aatgatttga ctcttcaaag aacgatgtct gcgtttcatt tggagactca aaccttcata
     5041 ctcgttagcg ggcgatgtgt ctttttgcat aaatggatga ttcaagattt tcgttcctga
     5101 tcctcgctgc gaatagacaa gtccttccat tgctaagata ttgatggctt ttttgattgt
     5161 catccggctc gcaccaaatt cttttaccaa gtcgacttga ttaggcaaaa aactatcgag
     5221 aggatactct tttgaacgga tacgttctct taaaatattt gcgatttctt cgtattttgg
     5281 cacgttattc acctcttcta gagtataacg aaagattatg cttccgacaa ctcgaagtag
     5341 catctggaac attgtttatt cgaaaaaaga ggtcatgaca agtttttgtc acaacccctt
     5401 aatgatctat taagcaatat ctaaatctgg ttgtttatct agtactttca ggaatgggta
     5461 ccagataaag gcaacgatga caaagtcgat gacttgcagc ataccaccaa gccatgaatt
     5521 tgttgctaag acaccgcttg caatgattgg cacagtccat ggaacagaga cgccagttgg
     5581 tgcagggaca agtccagcag ccattaccaa ataattgaac gtcgttacga ttagaggtgc
     5641 cacaacccaa ggaataagga ttgttgcatt caatacgatc ggtagaccga agatcgctgg
     5701 ttcatttaca ttgaaaatac caggtgctaa agctaatcgt ccaacatcac gatactgttt
     5761 ctttttaaga acaaaggcca tcaataaaac gacagctaaa gtcattccag agccaccaat
     5821 accaacagtg aaggtttcca tgaatggttt tgtgatgatg tgtggtaatt cttttccatg
     5881 ttgataagct tctaagttat caagcatcag tgtattccaa atcgggtcca ttacagagtt
     5941 gacgatgatt tgtccgtgaa gtccgaagaa ccataagaat tggacaaaga acaaagcaat
     6001 caatgttgca ggtaaactac ttcccaaacc agttaatggt ttttggatca cttcataaac
     6061 gacgtcatgt aagttagcat tgaaaatacc agtcatcaca gcattgatta ctaagaaaat
     6121 agttagtgtc actacagcag ggatcaaagc ggcaaatgat tttgttactg caggcggaac
     6181 tccatcaggc attttgatct gccagccgcg tttcgtgatt ctgcagtaga tttctccagc
     6241 caagaaagca gcgatcattc caatgaacat tccttttgcg ccaagacgat caagtgaaag
     6301 cacaccagtc acatcttcac cgccagcggt tttcattgca aaaggcgtaa gaatcaagaa
     6361 gctggcaaaa gacacggcac caccgaaaat accttcaacg tcataagatt ttgataaata
     6421 atagccgata ccaaaagtaa caaaaaccga catgatgctc attgttgcat tttgcccatt
     6481 accgaataaa ttcgataaag ttccctttgt tgcgtcactg aaaaaaggca agttattgat
     6541 aacgacaacg atcgaaccga acatcgttaa agggaaggaa agcatgaaag catcccgcag
     6601 cactgtaagg taacgatttt gccctaataa attagctaag ggcatgattt tctcagaaag
     6661 cttgtccata aaaccgttca tttttattca cctcaaattt atttgcaatc gattacatac
     6721 aaagtataac gccaaaaaaa ttaaatgtct agtcattttc ataaaaagta ttgatttatt
     6781 ttttagggtt cgttatgatt acggtatcaa gaaaaaagag aggaagtaaa aaaatgaaaa
     6841 cctacacatt cccagaaaat ttttggtggg gttctgctgc tagcggaccg caaacagaag
     6901 gaagagtaag tgatgatggt aaaggagaaa atatttggga tcattggtat gaattagagc
     6961 ctgaaaaatt cttcgatcaa gtaggacctg aaaaaacttc gcaagtttac acaaaataca
     7021 aagaagatgt tcagttaatg aaagaaactg gccataacac atttcgtaca tctatccaat
     7081 ggagtcattt gattccagaa gggacaggtg aaatcaatca aaaagctgtt tctttttatc
     7141 gagatttttt tactgaattg ctggataatg gtattgaacc ttttgtgaac ctttaccatt
     7201 tcgatatgcc actaccactg caagaaaaag gcggatggtt atcaagagaa acggtcgacg
     7261 cttatgtttc ttatgcagca gcgtgctttg aactgtttgg agatctggtc aaaaaatggt
     7321 ttactcataa tgaaccaatc gtacctgtag aaggaggcta cttgtatcaa ttccactatc
     7381 caaatcatat caatatgaaa gaagcggtcc aagtaggcta tcatgagaat ctagcaagcg
     7441 caaaggtaat caaagcctac catgaaggcg gacatgatgg tgagatcgga atcatcttga
     7501 atctgacacc aagctatccg agagatgaaa cgaacccaga agatgtaaaa gcggcgaaga
     7561 tcgctgacgc attcttcaac cgatcattcc ttgaccctgc agtgaaagga gagtttccac
     7621 aagagttgat cgaaattgtc aaagaattgg ctataatgcc agagatccat gaaggagacc
     7681 tagaactcat ccgtgaaaac acgattgacc tgctaggaat caattactat cagcctcgtc
     7741 gaatcaaagc aaaagaaact ccaatcaatc acgaacatag ccccatgcca gatgattatt
     7801 ttgataatta cgacatgccc aataaaaaaa tgaatccata tcgtggatgg gaaatttacg
     7861 aaaaaggaat ctatgatatt ttaacgaata caagagaaaa ctatggtaat atcaaatgct
     7921 atatttctga aaatgggatg ggggtcgaag gagaagaacg atttgttaac agcgaaggaa
     7981 ttattgaaga tgattaccgg atcgagtttg ttacggatca tttgaaatac gtccatcaag
     8041 caatccaaga aggaaccaat tgtgtgggtt accacatgtg gacatgtatg gacaactggt
     8101 cttggacaaa tgcttataag aatcgctatg gctttatcgc tgttgatttg gcacaagacg
     8161 gtaaacggac gatcaaaaaa tcaggtcgct ggtttaaaac ggtctctgaa aaaaacggat
     8221 ttgatgcctg atattttttg ataaaacgtt cacaaaatgt ttgttctaac actgtaatcg
     8281 tgctacacta acagtgaagg aggcgtttac aatggtagat acttatcgta atattttagt
     8341 agcaattgat ggttctgaaa aggcagaaaa agcatttctt gaggcaatca cgattgccaa
     8401 acgaaatcag gcaacgttgc acatcctgta tgtcaatgaa gtaacaggca attactttgg
     8461 tgattttgca tttgttacaa caagccttca ggaagaattg gatgaggttg cggaaaacca
     8521 gatgaaagaa catcgaaact tggcaattga aaaaggcctg acagatatcg agacatatgt
     8581 tctttacggt tatccaaaga cactgattgc taatttccat gaatcgaaag aaccaatcga
     8641 tctgattgtc atgggggcga caggtctgaa tgcggtagaa cgtgcactag taggttccac
     8701 tacttcctat gtagtcaacc acgcatcatg taacgtcatg gttgtaaaat aaataaatta
     8761 agggaacaga aagcaaaaaa gcataacgct tcttttgctt tctgttcctt ttattttttt
     8821 gtaaaaaatt actttacctg atgaagtaat catgctattc taattacaac gacagatgga
     8881 gtaatatgga ggaaagaaaa tgatttcaag tgatggaata cgcggataca atgatgcgat
     8941 tattttatcg atccttaaca aaaacgattc ttatggatat gaaatttcta aaacgattcg
     9001 tgagaggaca aatgattcct atgtgatcaa agagacaaca ctttactccg tttttgcccg
     9061 tttagaaaag aatcaactga ttgtttcgta tcctggagaa gtgactcatg gaaaaaagcg
     9121 gacatattat aaaatcactg aaatagggcg taatctttta gaagaaaaaa agcaagagtg
     9181 gctgacgacg aaagacgtag tcgatcaatt tttggaggga aattaaatgg atacaatccg
     9241 taattattta gacagtcttt ttatcggagt acctcaaagt accgagatag ataagctgaa
     9301 aactgatttg ttggcaaata tggaagacca ttatcatgaa ttgatgggag aaggaaaaaa
     9361 tgaacaggag gccatcggta cagtaatttc gacatttggt tcaatcgatg aattattaga
     9421 agaactagat gtcgaaaaaa aacatcaagc tgacgagacg gaaacaaaca cagcttctat
     9481 ttatttaagc gaagcagaaa actactggaa agaatacaga gcggcaagtc tccaagtggc
     9541 atcaggtgtt ctattcatta gcttatcttt tgcaagtttt cttttctttt gttcagcagg
     9601 atatgtgttc atgggtattt cttgtctgat tttcggtata gcattagctg tcggtttttt
     9661 tattgcttct ggtatgaaaa tcacacgttt aaatcacttt ttgcaccatc ggaaaatacc
     9721 tgaaaaagta ttagcagaag caaaagaaaa agaagaagag tatcagcgaa gttttggctt
     9781 ctcactgatt gcaggaattg gattatgtat tttttcactt ttcccacttt tggcttcttt
     9841 aatgtggtat atggatggta gtatcggtgc ctctatattt tttgttacgg taggaacggg
     9901 tgtcttcttg atcatttatg gcagtctagt ccgtcattct tatcgtcaat tcactcaaag
     9961 cgcttattac tggtgaatgt attcagattt gtaaaaatat tctatttatc cggcgtagaa
    10021 aaaaagactg tgacacgctt ttgtcacagt cttttttata tataataatt taaaaattca
    10081 tcatctaaat ccgtagaatt ttctagattg cttacccatg taaaatcgtt atagacagat
    10141 ttcagttgac ttctgcgcca ttgtacaggg ataaaaagac gcatgctatc ttcattgaat
    10201 ccgactaata atttctttcc gtcagtaatg atgggacgtt gtaacaaagt aaaatgcgag
    10261 acgatcagat cgacaagctc tgaggtcgta cattggtcga tttggtcttt caaattttta
    10321 taatcaatcg agcgtttagc taatagatcg tcaaatccgt tttctgttaa tgacaataaa
    10381 taaaatatct ctttccttgt taaaggctgt ttaggtgtga aaattcgttc tctgtaaggc
    10441 atttgatggt ctttcagcca gtttgccatc agatggtaac tatgtgtgcg agaagaatag
    10501 atcgtaagca taaaaaaact ccttaaatag tccattaatt tgttagatct gttgagataa
    10561 ataataggta aggagctgtg cataagcatt gatcaaaaca acccaaacat cattagaaat
    10621 aactttctaa agtcttaaac ttgcgacgag aaacaactag actaatagaa aaaatgtctt
    10681 agccatttgg cttcctgttt gtttttttgc ttatgctgca gctcctcggg aaagggttct
    10741 aacaatttga agaaggttga tgattgatct agtttaaatt gtgtgaaatt tgaaatctgg
    10801 tgtttttact agatgaatca cctaacaact atgattataa cttatagaac ttctgttttg
    10861 cattcggttt tgttatagtt gttataaagt tttattttat tacagaacat ttttaattaa
    10921 taaagaacag ttagaagtta aaattgctga ttcacataga gtttatgact tcttggaaat
    10981 tctttttcaa tcagtttttt catctgttta tgaaattctt ttttttcagt aaacataaag
//
LOCUS       DACDIH010000082        11032 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDIH010000082 DACDIH010000000
VERSION     DACDIH010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.082"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(89..1669)
                     /locus_tag="IV281_002339"
     CDS             complement(89..1669)
                     /locus_tag="IV281_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP8781310.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2070..3446)
                     /locus_tag="IV281_002340"
     CDS             complement(2070..3446)
                     /locus_tag="IV281_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8781311.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3574..4692)
                     /locus_tag="IV281_002341"
     CDS             complement(3574..4692)
                     /locus_tag="IV281_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8781312.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4834..5268
                     /locus_tag="IV281_002342"
     CDS             4834..5268
                     /locus_tag="IV281_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8781313.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5346..5879)
                     /locus_tag="IV281_002343"
     CDS             complement(5346..5879)
                     /locus_tag="IV281_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP8781314.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(5973..7151)
                     /gene="mutY"
                     /locus_tag="IV281_002344"
     CDS             complement(5973..7151)
                     /gene="mutY"
                     /locus_tag="IV281_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP8781315.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7144..7941)
                     /gene="recX"
                     /locus_tag="IV281_002345"
     CDS             complement(7144..7941)
                     /gene="recX"
                     /locus_tag="IV281_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP8781316.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8048..9430
                     /gene="rlmD"
                     /locus_tag="IV281_002346"
     CDS             8048..9430
                     /gene="rlmD"
                     /locus_tag="IV281_002346"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8781317.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9604..10182)
                     /locus_tag="IV281_002347"
     CDS             complement(9604..10182)
                     /locus_tag="IV281_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP8781318.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10504..10974
                     /locus_tag="IV281_002348"
     CDS             10504..10974
                     /locus_tag="IV281_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP8781319.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT         3283 a         2218 c         1903 g         3628 t
ORIGIN      
        1 ccgtcacggc tctaaacaaa atatgagagg ctgtttttgt ttggctattc ttattacttg
       61 atgcagggca gcttgtgtca agacttcttt acatcaaact ttttaatttt acattgggat
      121 atttatccgc aaaccagcgt tctgcaaatt ggttctcgaa taagaacaac ggttggtcga
      181 aacggtcacg agccaagata ttgcgacttg aactcatctt ctcatccaaa tcttctggat
      241 cgatccagcg agcgattttg tgtcccattg gagacatgat tacttcggcg ttatactcat
      301 ttaacatacg atattggaat acttcgaatt gtagctgacc aacagctcca atgatatatt
      361 cttccgttag atatgtttta taaagctgaa tcgctccttc ttgaactaat tgatagattc
      421 ctttgtggaa ggatttttgt ttcatgacat tttttgcagt cactttcata aacagctctg
      481 gagtaaatga agggagttct tcataagcaa cttttagttt tccttcgaac aatgtatccc
      541 caatctgata attgcctgta tcatatacac cgatgatatc gcctgctact gctttttcga
      601 cgttttctct cgaatctgcc ataaattgtg tgacattgct taatttcagt tttttatttg
      661 tacgttccaa ccagacatcc attcctcgtt cgaatgttcc agaacagatt cggacaaaag
      721 cgattcggtc acgatgggct ggattcatat ttgcttggat cttgaatacg aaacctgaaa
      781 attcttcctc ataaggactt accatctgat catcttgtgt tttgtgtcca taaggagctg
      841 gtgccaattc aacgaaagtc tctaaaaaag tctgtacacc gaagttagtc aatgcagaac
      901 caaaaaatac tggtgtctga tcgccacgag caattttttc ttgattaaat gcatctccag
      961 cctctttgat tagttctact tcttctaaga cttgttcata taaggaattt tcagttagag
     1021 gatgattttc gggaagcagt ccatcttcat ccaattctac taatcgttca tcctgataat
     1081 tttccggacg ataaaactct acacgattat ggtagatatc ataaagacct tctagccctt
     1141 tacccatacc gattggccaa ttcatcggat aagattcgat atctaatagc tcttctaatt
     1201 cttctaacaa ttccaatggt tcacgaccat cacgatctag tttgttgata aaagtaaata
     1261 tcgggatgcc acgttttttt acaacttgga ataatttctt tgtctgtgct tcgatccctt
     1321 ttgcactatc gattaccatg accgcactat ccacggccat caaagtacga taagtatctt
     1381 cagaaaaatc ttcatgtcct ggtgtatcca agatatttac tcgtttccct tgataatcaa
     1441 actgcataac agaactagta acagaaatcc cacgttgttt ctcgatttcc atccagtctg
     1501 atttggcaaa attacctgtt tttttgcctt ttacggtccc ggcttgacgg atagccccac
     1561 caaacaatag taattgttct gtaatcgttg ttttcccagc atccggatgg gaaatgatcg
     1621 caaaagttct gcgattgtcc acttgttgct ttaattttgg attattcata acctaactcc
     1681 atttcttcca ttcaattatt tatattttct ctaggcacag attgtccgat ctcttaaaga
     1741 aagacaattt catatagggc tgtgacaaaa gccgtgtagc agtctctttt tccgagtaaa
     1801 cggcgttcta gagacagcct atcagcaata agcccaccca agccaaaaat acgaaaagct
     1861 gtttttgctt ggctgctctt attacttaat gcaggatagc tttttcacaa cctttgtcaa
     1921 ctactacaac tgaatcagta tagcatggat tcagcgaatg aaaaagccac caaaccataa
     1981 gagtcaaata tttttttgtc agctgactaa aagaaaagac gtgttgcttg tttattcaag
     2041 caacacgtct ttcataacca agtttccaat tatcttcttg gctcgtcatc ttcgaattct
     2101 tttctgaagt atcttcgttc ttcttcaggt tccgcttctg tttcttccgg atcatgaaaa
     2161 atgacacgga ttttaaggat tcgagaacct tccatttctt cagaaaccag cgtaatatta
     2221 tcgacatcaa acgataactt ttcgccttca tccggaatca tgcctaatgc tgtaatcaaa
     2281 taaccagcca tcgtatctac atcactcata tgcagatcac tgtcaaaagc ttcattgaac
     2341 tcatcaatca gcatacgtcc ttggatgata tattcgtgtt catcgatttt ttcataaaga
     2401 ttctcaactt catctgattc atcatcgatt tctccgacga tttcttctaa aaggtcttct
     2461 agtgttgcta acccaacgac gccgccatat tcatctaata aaatagccat ctgattctgc
     2521 gtctttttca tttcatataa cagatcatca ataaagaccg tttcaggaac gaataaaggc
     2581 tcctgcatga ttttcttgat atctaaatta tcaaagccga atttatgggc agcttttagt
     2641 aaattctttg tatgaaggat tccgactact ttatctttgt cttcgttata aacagggatt
     2701 cttgaatagt tttcggataa aacttcattc acattttctt ccacagtgtc gttgatatca
     2761 atcataaatg catccgttcg tgggaccatg acttctcggg caactttcgt atcaagagaa
     2821 aaaacccctt ggagcatttc taattcttca ttattcaata cgccttcgtt ttcaagcata
     2881 taacgcattt catctctggt catttttgaa tcttcatcat caaaagtcat cggcgtgata
     2941 cgcgccaata aacttgtgga agcagaaagc agccagacga agggcttagc tactacacca
     3001 atcaagcgta tcagccctga agtgaacttt gcgacttctt ctgatttatt taaagcaata
     3061 cgtttagggt acaattctcc aaaaacaatc gatacatagg tcaaaatagc cagcacaatg
     3121 atatttgcaa tattttttgc cgctgtgccg ccacctaata ccggagctaa ccgttccgat
     3181 aaagtattag ctagtgaagc accagacaaa atgttgacca aagtgattcc tacttggatt
     3241 gtcgatagga aattatttgg attctgcaat atttttaaaa gcttttgaga tttcacatct
     3301 ccttcatcag ctttttgctc gattcgattc ttattgatcg aaacaacagc aatttcggaa
     3361 gctgctaaaa aagcattgat caatgtcaaa acgatcaata ataaaatctg cgctaacagc
     3421 gactgactct cagggtcagc attcataatc gcattctctc cttataaaat taatttttag
     3481 tttacgatag aaaaaaacgt ggcactatag ccacgttaaa atggtatcac aaagcaattt
     3541 tctttgcaat aaaaacaagt gtactaaaac tacctacgag gtcgtagcaa ttttcttctt
     3601 gttttcattt tgcttgtctt ctttgacgat tttgattata taggaaatga tcgtcttgca
     3661 gattgcgtaa aaaggaacac ctaagaagat accaagcaat ccggcaatat ttccagctac
     3721 tagaagaacg ataatgatcg ttaatggatg gattttcagt gatttcccaa ttacattagg
     3781 ataaatgata ttcccatcca actgttggac gattagcaca accacacaac aaagtaatgc
     3841 ttttaccgga tcattgaaga ctgttactag aaatgcaggt gctagtccta gataaggtcc
     3901 taaatatgga attaggtttg tcaatcccgc aatgacccca aataagaaag cataatccac
     3961 accaattacc atatatccta aaaatgtgaa ggtaccaacg aataaacatt cgattgcttg
     4021 tccgctaatg taattagcca atgttttatt cagttgcccc aataactcca caatttggtt
     4081 tcttcttttt tctgggaaaa actgttggat gtttggcact agtttttctc cgtctttcaa
     4141 catatagaac aagatgaatg gtgctgtaat gattactacg gttgccgaag cgatcgttcc
     4201 aacgatcgaa cctaaactat tggatactcc actaaggaat tgctgaatga ttgtcccgta
     4261 agaaatgtct aattgctcaa aatatttatt gatatctact tccttgaaca aaggcaattc
     4321 ggctacctct tttaaccagc tttctacctg tttgatgaaa tcaggcatat tagatgccaa
     4381 tgaggaaatt tgctgtacta gacttgggat gacactaagt aaaatccaaa taattgctgc
     4441 aactaaaagg agcagcacaa ttgcgacagc ccatattctt ttgatttttg ctttcatcaa
     4501 cagcttgaca ataggattca ataaatagta aagaaagcct gcaatcagta caggtgcaaa
     4561 taacgtggaa aaaaatgtcc cgatcggttc gaaaataaag tttatttctg atgaaatcat
     4621 gatcaatgca gctatgatca ataattcaag cgaccaaaac attagtttag actgtctaag
     4681 tttttcaaac aatagatttc ctcctttctt tcttactagt atatattata ttcgttaaaa
     4741 attcttttat tcttttttta tttttgaaaa cttgccatac tttttatttg ttttattttc
     4801 cagtatacta tacgaaagga aggtggctag accatgaaag ttgtacaaac aaaagataca
     4861 atgagcaata tttacttaga tgctgtacgg atcaggcatc aagtttttgt agtcgaacag
     4921 ggtgtgcctt tgtctcgaga aatagacaaa gatgaagccc attgtatcca cttcgtcctg
     4981 tactcagata aaaaagaacc gcaaggaact gtacgtcttt tgcctttaga aaacggaaaa
     5041 atgaaattac agcggatggc gattctttct gaatacagac accaagggtt aggaaaaata
     5101 ttgattgaag aagcagaaaa ctttgctaag aatcaaggct ataatacgat tttattaggg
     5161 gcacagtcca cagctgaaac tttttacgaa aaactaggat atacagctta cggcgatcct
     5221 tttgaagatg ctggcatgcc tcatatttat atgaaaaaga ctttataaaa atttatttcg
     5281 aaaaaagcaa aaaacagcaa gcttttgaga aaaatctctc actggcttac tgttcttttt
     5341 tgtgtttact tacgcgacaa ttgcttgtaa cggttatacc aaatatcaat atagccttcc
     5401 gagaacgggc catctccatt attgatccag tcaaccagaa tcttcacatt ttcttttagg
     5461 atgaagtcaa tatcgttcgg gtaattcatc attttgctgt gcatctcata ttcatcgaca
     5521 tccaatagtc gtttctcgcc gtcaggaaat accttgatat ccaagtcata gtcgatatat
     5581 ttcaaggcct catcgtccag caaaaaaggt gaagctagat tacaataata ggaaaccccc
     5641 ttttctctga tcattgctat tacattgaac cagtattttt tatgaaaata aacaatcgct
     5701 ggttcgcgcg ttacccatcg gcgtccatca gattcagtga ctaatgtgtg atcattcacg
     5761 ccgatcatag agtgttcgct tgtttttaat accatggtat ctcgccacgt tcgatgcaat
     5821 ttgccgtcgt gcttgtaact ttggatcgtc acaaactctc cttcttttgg aactcccata
     5881 gcaacccttc cttaagcagc attttgtgct ttgtgtttgg gtgttcatta caccgacatc
     5941 ataattatta tatcaataaa aatgagcttt gcctagaaag gattcctatt gtttcgattt
     6001 ttctctaact gatctaccag tttcatttgg accttaggaa aaacgaccga atcaaatgca
     6061 tcaggcttca accacttcga agttttgttc tcggtaaatt cttgttctgc tccctcggtt
     6121 gcccgtccgt aaaaaaggag aacatgccat ttcagatgac taaaaatatg agtaacttct
     6181 cctaaatgtc gtgtctgcca aacaaagagt tcctgctttt caaatgggag attttgctct
     6241 tcttctgcta ctaggtcatc aaacagatcg atttctggct ttgtttccca ttcttttttc
     6301 aatcgctcat actcctcttg tgtcacttcc atcattggaa atgtccacat attcgccagc
     6361 aatttttggc tatctctttc ttcaaaataa tacgctccag aatgattttg aagagctgca
     6421 ctgatataat aaacgtcttt tggctttgct ttttttgttt ttacaggaaa actcgtttgg
     6481 attcctcgtt tgttagctaa acaaaaggct tggatcggac aagtctcaca ctttggagaa
     6541 gttggtgtac agatagctga acctagatcc atcattgctt ggttgaattc acctggatgc
     6601 ttttcatcaa tgattttccg catcgcttca tcaaagattt tcctgctaga agcttttgcg
     6661 atgtctgctt caatgcaaaa caatctgctg actactcgca tcacgttgcc gtcaactgca
     6721 ggttctggaa gtccaaatgc gatacttgca atcgcgcctg tcgtatatgg tcctattccc
     6781 ttcaatgagc taatttcttc aggcgtttgc ggcatttccc catcaaattc tgacatgatc
     6841 tgtttagcag cagcttggat gttccgggct cttgagtaat aaccaaggcc ctcccatgct
     6901 tttaaaagct tttcttctgg agcatttgct agctcttcaa ttgtaggaaa ccattccatg
     6961 aaacgataaa aataatcgat cactgtgtct actctggttt gctgaagcat gatctcagag
     7021 atccagatac gataaggatc acgattatag cgccaaggca ggtttctttt ttcttgttca
     7081 taccattgaa tgaactgatc ttgaaattct tttgtttctt tatctgtcca tttttcccat
     7141 tctttattca ctttcctctt ccttttccgt aatgaatcgc tggatcatat ccaaatcaaa
     7201 tcctttttga tataagcttt gctttacttt tagatttctt tttttaggtt caaaccttaa
     7261 atttcttcgc catagtttat ctccttgttt gactaaataa tcatattcta tttcttgatc
     7321 tgcttcttgc ggaagatcag ctatcgcttg ttggatcact tcttgagaaa agcctttcgt
     7381 catcaatgtt tgttggattt ttctcaataa ttctttttgg ctttttccgt gattttttcc
     7441 aaacgctttt tcggctgtct gatgtgcatt ttgaacttgg tcttcaaatg tatattggct
     7501 gagcgcttgt tcaatgattt caggtttcaa acctttttgc tgcatctgct gcgctaattt
     7561 ctttggtcct ttgtcactta agcgcatatt cgtgcgcaca taactttccc catagaccaa
     7621 atcatcgaca aggtccaatt cttttaagcg ggctacgatt ttatttcgat cttcttgaag
     7681 gatttccttt tcttttaagt atgttcggat ttctttttcc gatcgcaact gataacttaa
     7741 gtaattcatt gctaggtgaa aaccgaaatc agctccgcta ctcttcttga tctcttggat
     7801 agtttcttcg tctaattccc gtccttttaa taatcgatag cgtactaata catcttcaga
     7861 tacttgtaaa gcttctcctt cagataaatc tacgcgatag aaagggcctt tgcctttact
     7921 gattctcgta acggttaaca taaacatcct cctcgaacat atattctcta tcataccaca
     7981 gaaaatcccc gtaatcaggt attatatgtt agaataaacc acagaaaaaa gaaaaaggga
     8041 gatacttatg gcagaaacaa ttgtcaaaac tggacaaacg gttcctttga aaatcaagcg
     8101 tttaggcata aatggtgaag gaatcggtta tttcaaacgg ttgatcatat ttgttccata
     8161 tgcactacca aaagaagaag tactagtgaa aatcacgaaa gcaactcctc gctatgcaga
     8221 aggagaatta ataaaagtca aaaaaacaag caaagatcgc gtagtggcac catgtcctgt
     8281 ttactatgaa tgcggtggct gtcaattaca acacttagct tatcaagctc aattggattt
     8341 taaaaaggat ctgcttttgc aagcattgga aaaattcaaa ccagcaggct ttcgatctta
     8401 tcaacttttg ccgacgatcg ggatggatga gccttggcat tatagaaaca aagcacaatt
     8461 tcagttacga aaaaataagc agactcaaaa aattgaagct ggtttatacg aagcaaactc
     8521 tcatcagtta gtccctataa cagactgctt ggttcaagaa cctcgaacac aaaaagtaat
     8581 gaatactgtt gttcaactat tgaataaatt tgatgcaccc atctatgatg aacggacaaa
     8641 cagtggaatc ttccgcacaa tcatggttag aatcggtgtt aaaactgggg agttacaagt
     8701 tgtttttatt actcgcagta aaaaattccc gcaaaaaaac gcaatgatcc gtgagatcaa
     8761 tgaacgactg cccgaagttg tttcaatcat gcaaaatgtc caggcacaaa aaacgtctct
     8821 cgtgatgggg gatgaaacga tccacttatg gggaaaagaa gccattgaag aacaaatcaa
     8881 tgaagtgact ttcgatcttt ctcctcgtgc cttctttcaa ctcaatcctc agcagaccga
     8941 attgctttat ggcgaaggaa tcaaagcatt agatgtccag cctaatgaaa cgatcgtgga
     9001 tgcttattgc ggagttggga cgatcggctt aagtgtagca aaacaagcca aagaagtacg
     9061 gggaatggat accgttcctc aagcaatcgc tgatgcaaaa aaaaatgcgg aaagattagg
     9121 atatcacaac actcgatatg aagtcgggac agcagagcaa gttctgccta aatggttgaa
     9181 tgaaggtttt cagcctgatg gtatcatcgt agatccacca cgtacgggtt tggataacgc
     9241 attgatccaa tcattattga aataccctcc aaaaaaactt gtttatatct cctgtaatgt
     9301 ttctacactt gccagagatc tggttaattt aagtaaaaag ttcaagattg aatatttaca
     9361 gtccgtcgac atgtttcctc agactgcacg ttgtgaagtt gtcgtgaaga tgacgagact
     9421 cgctgactaa atgaaagagg ttgtgaaaaa gtctgccctg tatcaagtaa taagaacagc
     9481 caaacaaaaa tagctcttca tgttttatga atactgtact tgtgaccgaa gagttaacag
     9541 gaggacacta tttatttaaa aaaagggttg tgacatagtt tttgtcacag ccctttttga
     9601 ttattattgg atattcacgt ctatcacttg ataaaatgca tttgtcgtat cgctgatatt
     9661 ccatactgct aaaatcacat tatatccttt tcgatcctgc ggaagtgtga tcgtttggtt
     9721 gactgttttt ggtggaacag ttgctttgtc gtctattttt gtgatcaatt caaaatcact
     9781 aaatttcaaa ggttgattag gattccaagt tggtttggta atgtagtaat cccaagtact
     9841 tgtccgatgt tgtgctgtta aattccaagt aatatttaat gttcctggat taaccatgtt
     9901 tttatgccat ctgttagatg tctgttcatc taaaggctca aagccgctga ctcctgcact
     9961 agcaattttt ccatcaatga atgtattttt ggtagcttcg atactttgcg gttcccattg
    10021 tgctcttccg acattttgat ttaagtttcc tgctgctgat gttcctaggt atgcacggct
    10081 tcctggagaa gtaatataac catgcgcaaa acctggaatg ctaaaaccga acaaccctaa
    10141 actaactaac ccaataaaca ataacgtact aatctttttc atgtagaaac ctccttcatt
    10201 tgataaccac attgtaaacg ctttattgta ttaacacaaa cattatgctt ttataatttt
    10261 atgttgaagt aaaaaaggtg aaaaaagtaa gatgcagatt ataacagaag tagacgagaa
    10321 aagtacaaat agaaaacatg agaatcaaat catatgatct ttttcaaaaa catcattcat
    10381 ataaacaaat gttcaacttt ttaataacaa caattatcaa tttttttctt acttcacaaa
    10441 ctaaatacag gtcttgctgt gagccttcat ggtaaactaa tattatagaa aggagttagt
    10501 aaaatgaaga tgataaaaga tattcgtttt tgggtttgtg ttataggaat tgtcatatta
    10561 ggctttttat cagggttgct tagcggaaat cccggaaaat attactattc tttgcaactt
    10621 ccaccatttg caccacctag ctggatattt gggccgatgt ggaccttact gtatatattg
    10681 atgggtattt ctctttattt gttacttaat cacaacaaca aaaaacagcg aaacaatctt
    10741 gtcggtcttt ttgtgattca gttcattttt aattttatat ggtctgctct attttttaat
    10801 ttaagaaata ttttcattgc agcaatcgat attacacttt tagttatttt cttgtcagtg
    10861 cttatgtatc agttgtggct gcatcatcgc ctggcaatgt ggctcatgat tccttattat
    10921 ttatgggtac tattcgcaac tctattgaat tattcaattt attttttaaa ctaagcgcta
    10981 aaaagagacg tcctgtaatt taacacatag aaaaagtgcc acctaagtta ga
//
LOCUS       DACDIH010000083        10575 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDIH010000083 DACDIH010000000
VERSION     DACDIH010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.083"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(48..1244)
                     /locus_tag="IV281_002349"
     CDS             complement(48..1244)
                     /locus_tag="IV281_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP8781320.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(1368..2030)
                     /locus_tag="IV281_002350"
     CDS             complement(1368..2030)
                     /locus_tag="IV281_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP8781321.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            2258..2821
                     /locus_tag="IV281_002351"
     CDS             2258..2821
                     /locus_tag="IV281_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8781322.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2834..3118
                     /locus_tag="IV281_002352"
     CDS             2834..3118
                     /locus_tag="IV281_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781323.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            3137..3829
                     /locus_tag="IV281_002353"
     CDS             3137..3829
                     /locus_tag="IV281_002353"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP8781324.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            3841..4386
                     /locus_tag="IV281_002354"
     CDS             3841..4386
                     /locus_tag="IV281_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8781325.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            4657..7935
                     /locus_tag="IV281_002355"
     CDS             4657..7935
                     /locus_tag="IV281_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP8781326.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8476..10383)
                     /locus_tag="IV281_002356"
     CDS             complement(8476..10383)
                     /locus_tag="IV281_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP8781327.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
BASE COUNT         3287 a         1960 c         2175 g         3153 t
ORIGIN      
        1 aataaactgt tgttgggcgt ttgttttagt aacaagcgaa aggtgatcta ttgggttaaa
       61 tccaacagtt tttgtttcag atcttcttcc atgtgtccaa gctctacttc tgcatgacga
      121 cgtttttctt ttccttcttt ttggatacgc aatgtctctt ggattgtttc gatcaaatca
      181 ttttgagtgg tttgaagggt ctcgatatcc acgattccac gttcattttc tttcgctgtt
      241 tcgatggctg agattttcag catttctgaa tttttcttca ataagtcgtt tgttgtttca
      301 gagacttggc gttgagcagt tactgcatct ttttgacgaa gcaaggtcaa tgcgatcacg
      361 acttggtttt tccacagcgg aatggctgta gcgatagatg cttggatctt ttcagccaag
      421 gcctgatttg tgttttggat caaacggatt tgtggtgctt gctggatcgt gatctgtcta
      481 gctaagcgaa gatcatgcgt acgtttatcc agacgatcta ggaattgcgt gtaatcattg
      541 gcaatctgaa catccatctg atcgccagtt tcttctgctc ttttcatcgc ttctggaatg
      601 atcgttgttt gaagttcttc cattttcaat tctccagcag cgatataaat attcagtgca
      661 tcaaagtagt ctttgttttt ctggtaaagc tgttctaaca tcagattatc ttttagcaga
      721 ccgtcttttt ctttatctaa tttcactgaa atcttgtcaa tttgtgcacc aatcttttga
      781 tattttgctg tcacttcata gatcgattgt ttgaccttcc cgaagacacg ctggaagatg
      841 ttcccttctc cggcacgcag ttcgtcagga ttggcttctt ggagacgata catcagttca
      901 gttaaggaat ctcctacagg accaatatct tgtgcttgca catgattcaa catcgattgt
      961 gagaattcac ttaattttgc ttgggcagcc gatccgtagc tgatgacaga ctgtgcatca
     1021 ttagcatcga tttttgctgc tagctgttta gcttgttctt ggcgttccgc cggtagtttg
     1081 tcaattaaac gagcagctgt ctgttgctct tggagctgat caatctccgc ttgttgtgaa
     1141 gtggtcagcg tatctgttgg agttgaaaac gggttgttca ataaatcttc aagtgtatcg
     1201 ttaactggtg taacatcttt tgtttcagtc ggtttatgat ccataaaaaa ttcctccttg
     1261 ttgaatctgt tctcgattct tacatgaatg tctctacttt agaaaaagta ctcgaaacag
     1321 ccagacatca tctgaatgtt ccgagtcttc ttttatgaac aaaagagtta atccttttgt
     1381 tctggttcat atttattatc acgctttaag ctctgtttcg caacagatat ctctacgtcg
     1441 atatcatcta gatcctcagc aacgaatttt tgataatctt gtgcgattaa agctgacagc
     1501 tggtcgatga tctgcgcgct ttcttctagt ttttcatagg tctgtttgtt tttgatctca
     1561 tggtcattga tctcattata cttatttgtc aaatcaacta gattcggcag atgcgtataa
     1621 agaaactgac ttgcttcatg cagtcggttt ggttctttca ccaattcttt gaataaagct
     1681 ttcgctgctt tgactggatc atggcggaga tcgattgctt taagtttggc tgtctgctgc
     1741 atattcattt gcaattgatt gatctcattt tttgtttgat tcattgtttg tcgaaaaaat
     1801 gaaatttcat tcgctgtcat accgcttttt tcatagtgag tttcaagttc gtcagtcagt
     1861 gcaggaattt cttcttgttt tccctttttc ctgcgcgaac caaagagtcc ccacaaaact
     1921 aaaactacac ctatcaataa aagtcctaga acaaaagaaa aactattgtt tcggacaaag
     1981 agaaatagca agaccaatgc tagaagccac ggccaatttc gttttatcat aataaaccct
     2041 ccatgtcatt tatcgttgag tattgaattc ttttcgttat tcgattacat acttatttta
     2101 gcatacggac tactataaag agtatcggac taaagaagga ttttctatat ttagagaaaa
     2161 tctaaggttt atctatggta aactaataag gagtttttga gaaaaattga aaaatgaaaa
     2221 aaaagcagtt atgattacaa agaaagcagg agaagatatg ctgaacaatg agcaatttga
     2281 agaaaaaaca atcagtcgca aagaaatcta tagcggaaaa attattgatg ttgctgtaga
     2341 cgaagttcgt ttgccagatg gaggaacttc gaagagagaa ctggtatttc atcctggcgg
     2401 tgtcgggatc attgcttttg acgaacaaga tcgtctgctt cttgtcaaac aatttcgaaa
     2461 acctttagaa aaagtgattt tggagattcc agctggaaaa atcgatccag gagaaggaca
     2521 aaatcccgaa atgacagcag ctagagaatt agaagaagaa actggctatc gtgcgaagtc
     2581 gctgagccat cttacttcga tgtatctttc accaggtttt gcaaatgaag tccttcatat
     2641 ttatcatgca caaggggtag agaaggtcga gaatccttta gcacaagatg aagacgaagt
     2701 actggagttg tatcatctaa ctcttgaaga ggcgcaacaa gcgatgaaag atcagttgat
     2761 ttgtgatgca aagacgattt atgcgattca atactgggaa ctattgacca aaggaaagta
     2821 gagggatcgg cttatggcca aaggaccgct tattacgcga agcgaacttc gcaagcgaca
     2881 acaagcacaa gcaagcgagt cattaaaaaa acagcgaaaa gcagaaacag cttatcagca
     2941 agaagaaaaa aagattgcca gcttttatcg taaagaaagc aaaaagaata aaccaatcac
     3001 gaaaacaagg atcagcgaac gggaaaagac gaccaaatgg aactcttttt tgatgaaatc
     3061 tcttattatt gttatcctaa tgttatgtgt ggttttttta gcaatcgcat ttatatagaa
     3121 agaaggaatt aaaaagatga aaatcggtat tatcggcgca atggaagaag aagtaaaaat
     3181 tttgagagag aacctaagcg aaccattatc atgggaacgc gcaggcgctt tatttatttc
     3241 aggttcatta ggtaaccacg aagtgatcgt cgtacgttcc ggtataggaa aagtcttagc
     3301 atctattacc acaagtctgt tgatccaaca atacggcgtg aacatggtga tcaatacagg
     3361 ttcagctggc ggaatcggtg aaggcttaca agtaggtgac atcgttatat cagacaaagt
     3421 agcttatttt gatgcagatg caacaggatt tggctacaac ccaggacaat taccaggaat
     3481 gcctctatat tatgaagcaa gcacatattt gcgcagtgaa atggttaaag cagcaaaagc
     3541 aacgaatttg aacgcaaaag aaggattgat cgttacaggt gatacatttg tcgactcgcc
     3601 agataaaatc aaagaaatct taacgaattt ccctgaagca ttagcttgcg aaatggaagg
     3661 agccgctgtt ggacaaacag ctcgtcagtt caatatccca tttttgatcg tacgtgcaat
     3721 gagcgacaca gcagatcact cggcgacaca aagctttgac gaatttatcg aagacgcagg
     3781 caaacgatca gcagaaatgg tgatcgaatt tgtcaaacat ctcgtataag gaggactaag
     3841 atgaacgcat tgatctcgat tgactacaca tatgattttg ttgcagagga tggaaagctg
     3901 actactggga cagcaggaca agtaattgaa aaaaagctga ctgctcatac aaagaagttt
     3961 attgatcaaa aggattttgt cgtttttgcc attgatgcgc atgatccaaa agattccttt
     4021 catccggaga accgcttatt tcctccgcat aatgtgatag gaacgggtgg acgggaattg
     4081 tacggctctt taaaaagttt ataccaagac tatgagcaag aggaaaatgt ttattggata
     4141 gacaaacggc attattccgc ttttagtgga acagatctag acatccgttt gcgagaacgc
     4201 cagatcacag atatttattt gaccggcgta tgtacagata tctgtgtgtt gcatacagca
     4261 gtcgatgcgt ataatctagg atacaaactc catattttca aagatgccgt ggcaagtttc
     4321 gatcccgttg gacatgaatg ggcattgaga cattttgagt ccacactagg cgcagaaatt
     4381 ttataaaaaa acaaagtgat ttgataaaat aaaaagcaca aaataataaa agaagagcat
     4441 aaaacaaacg actttgtttt atgctctttt ttatacgtta ttttaaaaaa acttattctt
     4501 tttacgttaa taaatctctt tattatcaac aacttaagtt atattttttc tttttataaa
     4561 atacacttat tataattttg ttttttattg acagtaaaaa taaattaggt aatactttaa
     4621 gtaaacgctt tctaatttgt tacgggagga atacacttgt taaaaaaacg tataaaatac
     4681 agtgcgctaa tgatgaatct ccttatgctc agtaccccgg ttttaatttc tgtcgttgaa
     4741 gttgctgcta acgaacaaca gtctgtgaac gaagaaaatg cacacgtgaa cattgaaggg
     4801 atgtcaacaa tggaggaaat accaagaact agtgaagata tggatacgga agaaggaaat
     4861 gatcctatga tgaaagaatc agaaaatgaa aagacaaaaa agcaatctga agaagcaaat
     4921 gaagagtgga aaggggttgt tttcggagaa agcacaagtg ctagtaggta tgccattgaa
     4981 ccattagaag acggcgtgcg gctctcttcc acgaataacg gcggaaaatt ccagtcaagc
     5041 ggatcagatg gaatgaccta ttattatcaa gcaatcccca aagacatcaa ctttaggatg
     5101 cgggcgacga tcgaaataga atcttggacc tatacgaatg cccaagaagg atttgcttta
     5161 atggtaagag acgcagtacc aaaaaatcat ttgtttgggc aggcatttta ttcaaattca
     5221 tttgccatac taggcagtcg gatcgaatat gtctgggact cggatagaca agaagtggtc
     5281 aacacgagcg gcagcaaata tacgatgcga cttggattgg ggacacgggc aatcacagga
     5341 atatcatctg aagatccgca agctgcgcca gctcctggga gtgtctcagt ggaaacaaca
     5401 cctttagaga cttcagcagg gttcttggaa aaagaaacag gttcttataa tatattcgga
     5461 aacggtcatg ggaatctgtt tccggccacg aactcgatta ctaaactgga cgtagagatg
     5521 aaaaaaacga atactggttt agaggcatat tattacgatc ctgaaacagg agaagaaatg
     5581 gcttctgaca tcatgtatga ctgggaaaaa ttatacgctt cagatgaatc ggttatttat
     5641 gcaggatttg ctgcagcaag gaatatgacg atcaaggtag aaggaatcga agttgcttac
     5701 tcagatcctg cgacagatcc tcctgggcaa gaaagaccta ctcgcttgat tgatccgatt
     5761 tacactgtga cctctagtaa tcattcagga aaccaagatc atactatgtc ctttagagca
     5821 aatgctgacg gattattgac aattaaaaat aaagcaacag gagaggtgct taagaacgca
     5881 aaaaacttgg ctatcactgc gaatcgggaa tttgattatc agacaaaact gatggaagga
     5941 acgaacgaat ttactttcag ttttacgcca gataaaaact atccgccaga agaatacgta
     6001 gagatgacta gttatgaaac aaaagagatt cttcatacag tggactaccg taaacctaaa
     6061 tattcgacag tttatgtaac accagaaggg tcagatgcag gaaacggaag cagacaaaat
     6121 ccattatctc tgacacaagc attgagatac gcatatgctg ggcagacaat cgtcatggcg
     6181 cctggaactt attccttcga aagaggattg acgattccaa aaggcgttaa cgggacaaga
     6241 gaaaacccga ttcagttgat tgcagagaaa aatccagaaa acaagcgtcc tgtacttgat
     6301 tttaaaggaa ctggaaatgg gatgacactg ttcagtcact actggggttt acgagggttt
     6361 gacattacaa atagtgcagc tatgcaaaaa ggattacaga tttctggaaa tcataatttg
     6421 atcgaagaga ttcatgctta tcggaatgga aatacaggaa tccaaatcag cggtagctca
     6481 aatgatcctt tcgaagcatg gcccgcgcat aacttagtca agaactgtac atcttttgaa
     6541 aatgccgatc caggatttga agatgccgat ggatttgcgg ccaaactgac aataggagaa
     6601 ggaaatgtat ttgacggttg tatcgcgtat cataatgcag atgacggttg ggaccttttt
     6661 gctaaaaatg aaacaggttc gatcggaaaa gtaatcatca aaaactcagt tgcttatcga
     6721 aacggctggg taccaggaat tgagggagaa ggtaacggta atggattcaa aatgggcggt
     6781 tcttctttaa cgggccctca tgaattgatc aatagtttgt ctttcgaaaa tcttgcgaaa
     6841 ggaatcgatt ccaatagcgg aacagatatt atcgtcaata gcagtacaag ttttaataac
     6901 ggttcatata atgtggctct ttatacctct tcagcaggaa acacagacta ccatgcttct
     6961 ggtatcttat ctttccgaac agagaatctt gagatgagag agcagatccg cccattgaat
     7021 caaaacgaag atcaaatcta ccactcgttg aactactatt ggaatgaacg agagaaacaa
     7081 tcagaaaata ctcttggaca taccatatca aaagattgga tcgagtctct aaatacatgt
     7141 tcgaaagaag gtcagcagcc tgttacgcgc tatgaaaatg gaagtatcca tgttcatgga
     7201 ctgatgcaga ttaaaccggg gaatcgtcaa acagaaaagg aaagagtgga tggtcttcca
     7261 ttatctgtag gtgctatttt tgacggagaa acctcaccgt cttcggaata tccagagtat
     7321 gcaatcgtac aagaaccaaa tcagacaaaa ccaaatgaac aaaataatcc gattattata
     7381 caagaagcaa atggtgtggc ggagaatgca gaaggaaata tcattgaact gaagcctttt
     7441 acagtttttg tgtcaacttt cacaccaaat caaggaacgc ctgtctttga agatatgcgt
     7501 tatttcccag aaaagatcca aatcgttgaa acacagtcaa gtgctcaatt ttctaatcat
     7561 accgctattt tttcagcacc ttcaaaagga gaatatacgc tagaagtcac ttatcgttcg
     7621 gaaatatttg atggggaaaa atggtctgct catacagaat ctgttaccca gcaaaaagcg
     7681 atcactgtgc gagaaactgc cgatgtgccc ccgggaaatg acaaaccaga aagagaaaat
     7741 ccaggaaatg aatcaccaaa tggaaaacca aatggaaaac cttctcatcc ggataggaca
     7801 gcaaaagaaa gtgaaaaaaa tggcactcaa ggactcccaa aaacgggaga atttaataac
     7861 ccgttgctag cattagctgg aggaattttg ttaatcggtg tagttgttta tgtcatgaaa
     7921 cagcgaaaaa aataagtgga atttctcctt ttatgatgta aaacaattga ctgaggaaag
     7981 taaaaataga aggaaatgag accaatagat gtctcacttc cttctatctt tttatagatg
     8041 aggttctcct tgctgctttt gattggctgt tcttattact tgatgcagga cagctttttc
     8101 acaatctctc cactaaacgg tgttcaatca gctgaccctc ggtattagct caacaaacgg
     8161 caaaatatga ggaactattt ttccgttttt ttctctaata ctcaggtcag gacgctgatt
     8221 tcacaacctc tcgataaacg gtgttcaaaa gccagtttct cggtagtaag tcaaatttat
     8281 gcaaaaatgt ggaaagccat ttttacaaaa tttttcttac tacttgaaac taagcagctt
     8341 tttcacaacc tctaatacat ggtattccag aagtaacttc ttcgaaaaat aagccgaaat
     8401 gtcacaaaaa tttgagaagc gattttccca aattccttct tatttcttga agctgaccac
     8461 ttctggcaca acctcttacc agattcgcac tcttttttct ggcgctagat acatagcgtc
     8521 ttgttccgtg ataccaaatg tttcatagaa ttcgtcaagg ttttttggtt gcacgtttgc
     8581 tcggagttta gcaggggcat ggacatcgat ttgcagtaaa agttgttggt attctttttt
     8641 tgcttttgta cgccaaatcg ttgcccaatt gacaaagaat tcctcgatag aaagatctga
     8701 ttccgttttt gctgcttcca atgcacagct tagaccgcct gcatcagcaa tattttccga
     8761 aacggttaat tttccgttta cttttccgtc agcaaaaggc agaccatcga attcttcgat
     8821 cattgcttgt gctttgtttt gaaattgtgt aagatcttct tctgtccacc agttgttcaa
     8881 gttgccatat tcgtcaaata aagcgccgtt attatcaaat gcatgggaaa tctcatgagc
     8941 gataacagct ccgattccac cataattagc agagcttgat tgttctagac tgtaaaatgg
     9001 tgcttgaagg atcgctgccg gaaagacgat cacattccga aatggatgat aataagcatt
     9061 gaccgtgtca gcactcattt cccattctga acgatccact ggtttgttcc atttgctgaa
     9121 acgatctttt aatgtgagtt tgctgaaaac aagagtattt gaaagcaaag taccaccctg
     9181 atctgcaggt actgttttga actggtcgaa taatgctggg atcttatcgg ggtagcctac
     9241 ttggatacct aagttattta attttatgac ggctttttct tttgttgcat ccccaagcca
     9301 gtcattttct tctagacgtt ttttataaac ggcgatcatt tttttcacca tttgttctac
     9361 gtctttttta gcagcttcgc caaaatattt ttttccgtag taatcgccaa ctacttgatc
     9421 gaattgtcca gaggcaaggt aatacgctgc ttttttctga ggcattgctt cgtctgttcc
     9481 agacaatacg cgagaataga ttccgctgac ttggcggaat tcttctgata gataaccact
     9541 aagagaacgt actgttttaa tgatcatcca gcttttgaat aacggaaaat gagcttctgt
     9601 caggatgtca ttcaaatgat cgaagtacac tggatcagtt acgatcactt tatctggagt
     9661 tgtcccaatc aaacccgtga caagtttttt caagtccagt ttatctgtgt atttaataaa
     9721 tgtatcaaaa gattgcggat tatacatttt gctgtaatct gcgttttctt ctgcactttt
     9781 tacatgagga gcaatcaatt tatcaaattg cactgcttgt tccgtgattg cttctgcctg
     9841 acttttttct ttgccggcaa gctctaataa ctgaacagcc atatcaaaga aaacgcctat
     9901 tagttgtgca ccgttgggat ggtctgatgc ataatacgtc ttatctggaa ggaataaaga
     9961 aggcggataa gcaaagaagg cgttcgtttg cgcattcttc atatctgcat ctacatccag
    10021 attaaatggc aatggtaatg tatccaacag ccattctgga aattgcttat tcaattcgtc
    10081 gaaagactgc aatgattcaa ctttttcgat tataggttga agcggttgag catgcaactc
    10141 atttcttcgg tcataatcgt ttgccagttg ataatatgca aggaaatgtt tcattttttc
    10201 tgaagacact ttttctggat gggcaagcag atcgtctgtt tctcccatca aaagttggtc
    10261 gatcccgtca actaaatctt ggaaaccgcc tgtagctggt ttatctgcag ggatttttgc
    10321 tgttttttgc cattcttcat tgactgcttc atagaaatct tgtttcaatc gttctttgtt
    10381 cattcatcga tcgctccttt tttgtttcgt gtgcccatta tcttacaaaa tgaaggaatt
    10441 aacaaataga acataaaaaa ttgaaaattc gaacaatcaa actgatttgt ttttttcaat
    10501 ccttcactta taatgaaaaa aaggaggcga tcccaactga atcaagtagt ggataaacct
    10561 ttatatttca aagta
//
LOCUS       DACDIH010000084        10551 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDIH010000084 DACDIH010000000
VERSION     DACDIH010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.084"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1451)
                     /gene="cls"
                     /locus_tag="IV281_002357"
     CDS             complement(<1..1451)
                     /gene="cls"
                     /locus_tag="IV281_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8781328.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1792..4458
                     /locus_tag="IV281_002358"
     CDS             1792..4458
                     /locus_tag="IV281_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8781329.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4822..5574
                     /locus_tag="IV281_002359"
     CDS             4822..5574
                     /locus_tag="IV281_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8781330.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5596..6750)
                     /locus_tag="IV281_002360"
     CDS             complement(5596..6750)
                     /locus_tag="IV281_002360"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP8781331.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            6935..9376
                     /locus_tag="IV281_002361"
     CDS             6935..9376
                     /locus_tag="IV281_002361"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP8781332.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9706..10410)
                     /locus_tag="IV281_002362"
     CDS             complement(9706..10410)
                     /locus_tag="IV281_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781333.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         3372 a         1894 c         2219 g         3066 t
ORIGIN      
        1 tataggattg gagaaaataa tcgagaaatt tgttgcttga ataatagcca gttagacatt
       61 tccttgactg tttcagttgt catggatgaa caatgtgtct catcttcttt gaactgagta
      121 gcaagttctt ctaaaaactc aggatcgtaa atgacagcac ttgtttcaaa attcaatcgg
      181 tagctacgaa tatcttgatt tgctgaacct accatgcaga tttcatcatc cattatcaat
      241 gtttttgcat ggaggaagcc gttctcatag acaaggattt gtacattttc cttgatcagc
      301 tgccgagcat aatattgtgt tgctcgataa ataaaaggat gatccggttt gttgggaatc
      361 atgattttca catctacacc agaggcagtt gcgattttta aagcagcaat gacactttca
      421 tcaggaacaa ggtaaggtgt ctgtatccaa acacgttttt tagcagaagt aatcaatttg
      481 ataaatgaaa gcttgatttg ttccctttcg ttattcggtc cgcttgaaac aagctggatg
      541 gatgtatcag caagcttaga ttcatctcga tctgcttttt taaagaaata attcaaatga
      601 tagccgacct ttttttcttc aggtaccgag acgttccagt ctgttaagaa gcgaagctgg
      661 agcaaagaag aagcggcacc gaaaatccgt atatgcgtat cgcgccaata gccgaatttt
      721 tttgttgtat ttacatattg attggcaata ttgaaaccac cggtataact aatctttcca
      781 tcgatcacaa cgatttttcg gtgatcatgg taattcaatc ggaaacgaag taatgccctt
      841 tgtgaggtaa caaatgtatg gacatgtcca ccatttttga ttagacgatt gaaatctttt
      901 gcttttgtgc catgagagcc aaatgcatca tataatattc gaacttccac gccttctgcg
      961 gctttttctt ctaaaacatg taagacttgc tggccgatat tatctgtcac aaatgcgtaa
     1021 tattcgatat ggatcgaatg tttggctttt tttagatctt gaagcagtga atccaatttc
     1081 tcttgtccgt ctgtgagaag agtgacagaa ttcattcttg tcagcggcat acgatttaat
     1141 gaagagaaga agtcaacaaa ttgctgtttg tctttatcgc ccatgtcagg atcgtaatgt
     1201 tcaatggtat ctcctctaaa agactgaaag ttttctaatt ctttcaagtc actttgtcgg
     1261 agatagaatt ttttcttatc cgttaatcca cgtccgaaaa ataaatataa tacaaagccc
     1321 accccaggaa gggcaaacaa tactaagagc catgcccaaa tggctgctac atctcggggt
     1381 ttgatcaaaa tagccaccaa agcaataagt gcatttaata gataaagggc ggttataata
     1441 ctagatacca caaaaatgtt cctccttaca tacaataaca ttagtatagc atgatagaag
     1501 aaagaagcct accaaggtga atatactttt cactcctttc atgaaaatgt aggtaaatcg
     1561 ggctaaagta gttgcttttt gtaggttaat aaggtaggat attagtacag ttgaatatct
     1621 ccttagggga gtaaccggca acgtttgttg cgataatatc aacagtaagt taaccaaatg
     1681 gttttctggt attgtatgaa atggtgagac ctaagacaaa tgcgtatttg tcttaggtct
     1741 cttttttttc agacaggact agggccaaga tcaaaaaatg ggggatgaaa tttggaaaac
     1801 aaggaacaag cacaacaagc ggtctataaa gaaacgatcg actcgttgat gcagcaaacg
     1861 cagagctcaa ccgatggatt gagcaccacg caagcgaagg aaaaattaga aagagatggc
     1921 ttgaaccagc taaaagaaaa gccgaagaag ccaacttgga aattgttctt agaaactttc
     1981 aaagatgcaa tggttatcgt attgctgatt gtcgcactga ttcagatggt catgggtgac
     2041 tacatcgaat tcttagttat ttttgcagta ttgatgttga attctataat cagtgttgtc
     2101 caaacaaaaa aagcagaagg ttctttagaa gcgttgaaaa acctttcagc gcccgatgct
     2161 cgagtgattc gtgatggtaa ggaaaaaacg attccggcaa acgaacttgt ggtaggagat
     2221 atcgtgttgt tagacgcagg cgattatgta cctgctgatg gacgtttact agatgcggga
     2281 tcattaaaag tagatgaagg tatgctgaca ggagagtcga cttctgcaga aaaagaagta
     2341 acggatatca caagtacggt accaatcgga gatcgtacaa atatggtatt cagtggaacg
     2401 atcgtaactt acggacgcgg taaattctta gtgacttcaa ctgggaataa tacggagatc
     2461 ggacaagttg ctggcctttt ggaatcaaca actgagcaag tcactccgtt acaacgaggt
     2521 ctggataaat tcagtaaaaa actaagtttg gctattttgg cattgtcttt attgatttta
     2581 ggtatccaac tgtttcgtat ttatcttgga gaaggtacag gagatatgac tgcagatatc
     2641 gtcagcgcag tgatgtttgc cgtagccgta gccgtagctg ctattccaga agcattacag
     2701 tctatcgtaa caatcgtgtt atctttggga acgaataaaa tggcaaaacg tcatgccatt
     2761 attcggaagc ttccagcagt tgaaacactt ggttcgacaa gtatcatctg tacagataag
     2821 actggtacat tgacacaaaa caaaatgaca gttgttgact actttttagg taatggcgat
     2881 actggggatt tcacaaatga cccttcaaaa tggacagctg acgaacgtcg cttgatcgaa
     2941 atcagtgttt tagcgaatga tgcatcgatc agtgaagacg gtactaaatt gggcgaccct
     3001 acggaagtag cgtttatcga ctttagtgaa aaattaaacc agccttatca ggaaatccga
     3061 aacaactacc caagacaagc agaacttcct tttgactcag atcgaaaatt aatgtctact
     3121 ggacacacaa ttgataacaa aaattacttg tttgttaaag gcggaccaga tatcgtcttc
     3181 caacgcagta aaaaagtatt gctaaatggt gaagtcgttc cgatgacgga tgaattgatg
     3241 aaaaaattcc aagagcaaaa tgaagctttt tcaacacgcg cattacgtgt actagctttt
     3301 gcttaccgtc cacttgataa ttttgattta tcatttgaag atgaagacga tttgatcctt
     3361 gttggtttgc ttgctatgat cgatcctcca cgtgctgaag tgactcaggc ggtaacagaa
     3421 gcaagaagtg caggaatcaa aacaatcatg attactggtg accacaaaac aacagctgtt
     3481 gctatcgcaa aacaaatcgg gatttttgaa gaaggaaacc tggctttgac cgggacggaa
     3541 ctagatgaat tgacagaaga tcaactaaac gaagttttag aaaaagtcac tgtttatgca
     3601 cgtgtttcac ctgagaataa aattcgtatc gttcgtgctt ggcaaaataa aggtcatatc
     3661 tcagctatga caggtgatgg tgtaaatgac gcaccagcat tgaaacaagc agacatcgga
     3721 attgctatgg gaacgggtac agatgtggca aaagatgcag cagcgatggt attgacggat
     3781 gataactttg cttctattgt cagcgcagta gaagtaggac gtaatgtgtt tgataatatc
     3841 aagaaagcta tttcttatct atttgcagga aatttaggtg cgatcattgc gatcatcttt
     3901 gccttagttg ctgactggtc caatccattt acagcattac aactgttgtt catcaattta
     3961 gtcaatgatt cactgccagc aattgcatta ggtacagaaa aagctgaacc tgctacgatg
     4021 aaccgcccgc caagagatcc aaattcagga atctttacgg acaaaacatt gatttctgta
     4081 acgtatcgtg gtgtgctgat tgggattgct gttatctttg ctcaatggct tggcatgcag
     4141 cattcacctg aattcggtgt agccatgtca ttttcaacat tgatttggtg tcggacattg
     4201 caaacattcc cagcacgttc aaacacacaa acagctatcc aagcagggtt cttctctaac
     4261 agaagtgtct tactagctgt agttgtatgt agcgcgcttt actgcttaac aatgatccca
     4321 ggactgaaag aagtcttctc tatcccatca gcatttggat tgacagaagt ctttacatgt
     4381 atgggaatcg cacttgtgac aattgttttg atggaaatca ctaaattgat cattgtacga
     4441 gtacaaggaa ataaataaaa cgaatcaacg aggttgtgag cctgccgttt aattctgagt
     4501 cacaaggtgc aatttcaaca aacagcttct catgtttgtt gaatattgtg acttgtgacc
     4561 gaagagttgg caggagaaca ccgtttaagg aagaagttgt aaaatcagca ttttgagcta
     4621 atactgaggg tcatctgatt ggacactgtt tattcggaaa gagggacttt gacacgactt
     4681 tggtcacagt cccttttttt atttatgata gacggaaagc caatcatttt attattcatc
     4741 aaaagagata gaatagataa aagaggagaa tgatttattc cgaaaagtga aaacatataa
     4801 caaaaaatta gaggaaatca aatgaatgaa aaagaatggg atgaatttgc tgaagaatat
     4861 tatgaaatcc agcaagaatc gcaagtaact attgctgaag atgtaaaaaa ttatctgaaa
     4921 acggagaaag tcctgcctgc tgaaacagca gcagacgttg ctggaggatc tggcagattt
     4981 ctttccttag caaaagaaat taatcgctat gatttgatcg atttttcttc aggaatgcta
     5041 gaaaatgcaa aaaaagaagc agaaaaaaat gagttgccaa atgtttcctt tatcaaacag
     5101 tcgttagaag aatttatcca atcaaatgag acatatgaaa tggtttttac agcagcaaat
     5161 ccagcattgg tacatcttat tcaattagaa aaattgatga aaaagtcaag caaatggtgc
     5221 gtgataatca gagtagtgga atcaatcgat gatgtttttt atcctatcga aaagcaacta
     5281 ggaatgatag aagaagatcc taatacatcg ccagttgtaa tggatcaatt tgaaacatac
     5341 ttaattgatg aaggattcaa agtgaagaaa aaagatttta cttattttac acaggaatca
     5401 attactgaag aattgatgaa gctatactat gacgcgtacg caaaagatcc tgttgtgacc
     5461 aggtacattc gcaaactatt cagtgaaaaa cgagaaatcc ttagtacgac taagctgacc
     5521 tatagactgc tcctttggtc cgcagataaa aaaagaacct ttcacgactc gtgaagggtt
     5581 ctttttatga ttttattata ttttgtagta acgaatatca ttttcgatct ttgtaatcga
     5641 aaaaggtaca tctgaagtat attctgcaca ttctccgtgt tcaggaagcg tctcactgaa
     5701 aatcgtttca tattcgtcaa cagtgatccg tgttcgagaa tctagcatct cttgatgaga
     5761 ttgtgcgaag agatattctt gatagttttc ttccagataa cctgtaaaaa attcacttac
     5821 tgcaccagaa ccgtagctga atagaccgat gcgatctcct ggctgtagtg atttcgaatt
     5881 ttccagtaga gaagttagcc ccaggtataa tgaaccagtg taaagattac cgattcgtcg
     5941 gctgtaacgg atgctttctt catagcgagc cataagacgt tcctgatttt cttcgtctgt
     6001 ttggtctaat acgctttgca atgccttttt tcccatctta gtatacggaa tatggaaagc
     6061 aatcgcctga taatcttcga gtcctcgacc cgacaattct ttatgtcgat tccaaacttt
     6121 ttggaatgat tcgatatacg tagaattaga taaaggacca tcaacaacag gaaattcgct
     6181 ataatctgga cgccagaaat catagatatc ttctgtcaga aatacgctgt cgtcttcaat
     6241 cgataaaata cgcgggtttt gagtgatcat catcgcaaca gcaccgacac cttgcgtcac
     6301 ttcaccaccg cttgccaagc cgtaacgagc aatatcactt gctatgacta gtacttttcg
     6361 ttctggatga tttttgacat attctttcgc catgtgcaaa gctgcggtgc caccgtagca
     6421 agcctctttt acttcaaaag aacgagcaaa agattggatt ttcagtaaat ggtggataat
     6481 taccgcactc gcttttgaat gatcaatccc ggattctgtt ccgacgatga ccatgtcgat
     6541 tagctcacgg tctttttctg ttacgatttt gctggcagca tttgctccta gtgtaatgat
     6601 gtcttcattt gcacgattga ctgccatttg gtcttgacca atccctatat ggtattttgc
     6661 aggatcatcc ccacggcttt ctgccaactc tgtcatatct aaatataaat taggaataaa
     6721 aaaggaaaga cgatcaatcc ctattttcat ttcaacacct ctttacttat gataaaaaac
     6781 ttactttact aaggtatcat aaaaagatta aaagaaatat aaaaaagaga atatttaagg
     6841 attgttaaaa tttaatctta gaaaaaaata actggaacaa gagctatttg tttttacagc
     6901 aaagaatggt atatttttgt aggaggtggc ttacttgaaa gaagtcgtta tgatagatgc
     6961 tgcaagaacg ccgataggaa agtatcgcgg aagtttaagt ccatttactg cagttgaatt
     7021 aggcacactt gtgactaaag gattattaga taaaacaaaa ctgaaaaaag ataaaatcga
     7081 tcaagtaata tttggaaatg tcctgcaagc tggtaatggt caaaatgttg ccagacagat
     7141 tgctttgaac agcggtctcc ctgtagatgt cccagcaatg acgatcaatg aagtttgcgg
     7201 atctggtatg aaagcagtga tcttagcacg ccagctgatc caattaggtg aagcagaact
     7261 tgtgatagcc ggcggaacag agagtatgtc tcaagcacct atgctgaaac agtatcagtc
     7321 agaaacaaat gaatatggtg aaccaatttc cagtatggtc aacgacggat tgactgacgc
     7381 attttcaaat gcacatatgg gattaaccgc agagaatgtt gcaacacaat tttctgtgag
     7441 cagagaagag caggatcgct atgccttgtc gtcccagttg aaagcagcac atgctgtcga
     7501 agccggtgta ttttctgagg agatcatccc agtcaagatt tctgatgaag acgtgttatc
     7561 tgaggatgaa gcagttcgtg gaaatagtac attggaaaaa ctgggcacgt tacgtacagt
     7621 attctcagaa gaaggaactg taacagcagg aaatgcttcc ccgttgaatg acggtgcctc
     7681 tgtggtgatc cttgcatcca aagaatacgc agaaaataat aatctgcctt atttagcaac
     7741 catcaaagaa gtagcggagg tcggtattga cccaagtatc atgggaatcg ctccaatcaa
     7801 agccattcaa aaattgacag atagatcagg aatgaattta tcaacaatcg atctttttga
     7861 gatcaatgaa gcatttgcag cttcttctat cgtagttagc caagaactgc agttagatga
     7921 agaaaaggtc aatatatatg gaggagcaat cgcattaggc catccaattg gtgcaagcgg
     7981 tgcacgtatc ttgactacat tagcttacgg attgcttcgt gaacagaaga gatacggtat
     8041 tgcttcatta tgtattggtg gaggtttagg cttggcagtc ttgttagaag cgaatatgga
     8101 gcaaacacat aaggatgtgc aaaaaaaaaa gttttatcag cttacgccat ctgaacggcg
     8161 cagtcaatta atagaaaaaa atgtattgac acaagaaact gcgttgattt ttcaggaaca
     8221 aacactttct gaagagctct ccgatcacat gatcgaaaac caagtgagtg aagtggaaat
     8281 cccaatgggg atcgcgcaga attttcaaat caatggcaag aaaaaatgga ttccgatggc
     8341 aacagaggaa ccatctgtca ttgcagctgc aagtaatggt gcaaaaatct gtggaaatat
     8401 ctgcgcagaa acacctcagc ggctgatgcg agggcaaatc gttctatcag gaaaatcgga
     8461 gtatcaagca gtgatcaatg cagttaatca tcgaaaagaa gagctgattc tttgtgcaaa
     8521 tgaaagttat ccttcaatcg tgaaacgcgg gggcggtgtt caagatatct ctactcggga
     8581 attcatgggg agtttccatg catacttatc tattgatttt ttagtggatg taaaagatgc
     8641 aatgggcgct aacatgatca actcgattct tgagagtgtg gcaaacaagt tacgggagtg
     8701 gttccctgaa gaagagattc tattcagtat tttaagtaat tttgctacag aatcattggc
     8761 ttctgcctgt tgtgaaatcc cattcgaacg cttagggaga aacaaagaaa tcggtgaaca
     8821 aattgccaaa aaaatccaac aggcagggga atatgcgaag cttgaccctt accgtgcagc
     8881 aacgcacaac aaagggatca tgaatggtat agaagctgta gtggcagcta caggaaatga
     8941 tacacgtgcg gttagcgctt cgatccacgc ctacgcggca agaaatggat tataccaagg
     9001 attaacagac tggcagatca aaggggataa actagttgga aaactaacgg tgcctttggc
     9061 tgttgccaca gtaggcggag cttccaatat cctgccaaaa gcaaaagcat ctttagctat
     9121 gttagatatt gattcagcca aggaactagc acaagtaatt gctgcagtag ggttagctca
     9181 aaacctggct gctttgcgtg cattagttac agaaggaatc caaaaaggcc acatgggact
     9241 tcaagctcgt tcattagcga tctcaattgg ggctattggt gaggaaatcg agcaagttgc
     9301 gaaaaaatta cgtgaagcag aaaagatgaa ccagcagaca gctatacaaa tattggaaaa
     9361 aatccgggaa aaataaaaaa gcaatatctt tagaagaata aagcaagatt tacagttatt
     9421 ttatttactg cagatcttgc ttttttctct atcagaaaat atgtttatta ttagaatttt
     9481 ttatagttaa ggttaggcat cttaagaaat caggcaactc caagatatct tcaggtagaa
     9541 gagaagtcgc ctaacattgt aggatcatag gtgatttgtt ggacaggtca agctgatatt
     9601 tgagcctgat gttagaagga atttcttttt acttgctgta gaaattatta tatgactaaa
     9661 aaggctggga ttttaaatcc cagcctttta tgacaaatag aatttttatc gatcagtaat
     9721 cgctgtcgct gcaaattgtt cgatcaaagg aatcaaagtc aatttgactt tgtatttccg
     9781 ttcgagtgct ttttcgataa cgaactcatt ttcagtagtc atagaaatag ttgctaattt
     9841 acgaggttca tcagcatttt tttcattaat aactacttca tgttcatttt gttctgtgta
     9901 aaatttagga tactcagcaa cggcttttga aaaatcagca aaacttaaag cggcattcgt
     9961 atcagttgag cgtgtatttt cagttgtgtc cgcttttggt ttttccgttt tttcttctgg
    10021 cacactgact gtttcttctg taactggtgt cgcaacagct tgcgcagttg gttcatcgtt
    10081 tttcaatcgt tgatcattcc agtaagacac gatttcaata agaagattgt cacggaatcg
    10141 ttcgaaaatc ccgttttctc ctgaacgaat tctctttaat acagtattga agttgtcatc
    10201 gattggcacg ttgatccgtg tatcttcatt tgttttccca gctaatccga tcgttaagat
    10261 ttgacggttt ttgggatcaa gcatgacttg taacggcaaa ccataacgtc gcaagctctc
    10321 ttcttctaga gaacggaata attgtcctct tacaccatcc aatgaaggac ggaattgatt
    10381 taataatttt tgtttctgtt ttttcttcat atgttctctc cttcgctcat accctttcga
    10441 ttataagaaa aaaacagcca aatgaaaagg agtaatgcaa aaaaacttgc gtatacgcaa
    10501 attacaacag aaaaatttgc gtattttccc gttttttgaa agaaaaataa a
//
LOCUS       DACDIH010000085        10148 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDIH010000085 DACDIH010000000
VERSION     DACDIH010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.085"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(244..753)
                     /locus_tag="IV281_002363"
     CDS             complement(244..753)
                     /locus_tag="IV281_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP8781334.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(954..1823)
                     /locus_tag="IV281_002364"
     CDS             complement(954..1823)
                     /locus_tag="IV281_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8781335.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(1843..2964)
                     /locus_tag="IV281_002365"
     CDS             complement(1843..2964)
                     /locus_tag="IV281_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP8781336.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(2966..3361)
                     /locus_tag="IV281_002366"
     CDS             complement(2966..3361)
                     /locus_tag="IV281_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8781337.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(3380..4177)
                     /locus_tag="IV281_002367"
     CDS             complement(3380..4177)
                     /locus_tag="IV281_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8781338.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(4179..4967)
                     /locus_tag="IV281_002368"
     CDS             complement(4179..4967)
                     /locus_tag="IV281_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8781339.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(4981..5457)
                     /locus_tag="IV281_002369"
     CDS             complement(4981..5457)
                     /locus_tag="IV281_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8781340.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(5529..8186)
                     /locus_tag="IV281_002370"
     CDS             complement(5529..8186)
                     /locus_tag="IV281_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8781341.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKR
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(8205..8609)
                     /locus_tag="IV281_002371"
     CDS             complement(8205..8609)
                     /locus_tag="IV281_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HAP8781342.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(8841..9098)
                     /locus_tag="IV281_002372"
     CDS             complement(8841..9098)
                     /locus_tag="IV281_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAP8781343.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(9223..9555)
                     /locus_tag="IV281_002373"
     CDS             complement(9223..9555)
                     /locus_tag="IV281_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781344.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT         3156 a         2058 c         1431 g         3503 t
ORIGIN      
        1 cagagtaaac tctgatttat tcgctaaata ctttttttgt tgtattaaag acatctttcc
       61 ccgaagctca aaattgagcc tacatcgatt ctctatctct tcaaaaagca tcttatcttc
      121 atcattctta tattcatcat tcataaaacc aatccagctc ctcgattaag ataatatttt
      181 aatctcatac aaaaactagc tctcatcaaa acaaccattg ttttgatttc tcatgaaatt
      241 tctttatact tttctccgat tataaattcc taaccccttg agaaaattac gcatatattt
      301 atctccgcaa ggcttaaaat ttcgttggcc ttcttttctc aaaaatgccc cgatttcgcc
      361 ttttgatgga tagatttctg cctcagcaaa aacgtctaaa atatcatcag tggtcataga
      421 caaggcgatt tttaattttt ttagcaacac attgttgata tatttgattt ccttgctttt
      481 ttgcggcgtg tctactatcg gcttatcttt ttgcggacca cgcgccaaga gaatcaggcc
      541 atttaggaat gcgtcaaaaa cttggttgtt gatggttttt tcatacacat tattttctgc
      601 attatctttg ttcttatcct gtactttggt cagtaccgcc tgagcttctt ctttagtcag
      661 tggtacaccg cctagctcaa aagctttaat caaatccgta tctttcaaat caatcgcata
      721 acgcaatcgc aataacctat cattgttatt catttgttcc tccgaagtat ttagtcttta
      781 ctttcttacg tactttttgt cttgctaaga tttttaggat caatgataaa aaccgcatca
      841 cttatgataa ggcgctgggt atcatgagtg aatgatattg ctgaatacaa atagtccgac
      901 cacattaagt tgatcggact tcataagcat tttattgact aatacttttt tgcttaagaa
      961 ttgattctaa catcttttgc taaagttaag catcctatag ttcctgcatt atctgctaac
     1021 ttaggtgtga caatgtattc ttcgatattt ggcgtatcga catagccgtt aattagttct
     1081 aaaaaacgtc tttgtacttt ctttttcaga tgttcttgtt gcatgacacc accaccaaaa
     1141 attataatat ctggtgaaag catcagggtc gtattacagg cacattgtgc aatataatcc
     1201 gcctcgattt cccagaacgg atcttctata gaaagatctt gtcccttggt tcccgtacgc
     1261 atctcgatag cgggtccaga tgccaaccct tcgagacaat catgatgaaa aggacaagta
     1321 ccagagaaag tatctcctga atgtctctta actaacgtat gccccatttc tggatggcta
     1381 aacccttcaa caaaaatccc atcttgtata gctcctccac caattccagt tccaacagtg
     1441 taataaacaa cactggataa accttttcca tgacctgaaa catactcccc gtaacaagca
     1501 gcattcacat cggttgtcca ggcaattgga atttcgaaat gcttattcaa cgttccaaca
     1561 aaatcaaaat ttttccaggc agtttttgga gtagaggtga tatgtccata ggtctttgat
     1621 tcacgatgga tatcaatcgg tccaaatgac cctactccaa tgctttctaa ttgttcttta
     1681 tagtgattaa agaaatcaat taccaaactc attgtttcat tcggtgtagt tgtaggaaaa
     1741 ctcactcttt ctaaaacctt taaatcatca ttgccaatgg cacaaacaaa tttagtccca
     1801 cctgcttcta ttgagccata catttcttac tccaccgtcc ttttatctag gaattcgttc
     1861 ttccctgtct aatagttttt cgaaaaactc atgtggttcc ttttggtacc aataggcaac
     1921 agaagaaata tcgtcttgac gctcataaag cttgatgtca tcatttccta gggtttggaa
     1981 agtaacacgt aaattttctt tgaaactaat ggggtcgggt aaatgccagc ggtacaaccc
     2041 atgcatcggt aaagcatcat cgccaaaacc atgaactgga tttggattag gttttccagc
     2101 actaaaataa tcccgggtgt gatctctttt agattgaaaa ggatagccca taaatagcgt
     2161 attaaaattt tgagcatatg ccctttctcc aaagttcttt ttatggaaag cccaagcgcc
     2221 gccaaaataa tcttctgctc ctgtagatgt tattgtaggg aaatcttcat ctccatctat
     2281 ataaaactta aattctcctt ccccccacca gtatctttcc aatgcgcaca aagcaaagta
     2341 ggttcctaca taatacccag tgcctttgat tccatcaata agggtgtaat cttttccttt
     2401 tgtcgtgatg ggttcacggt tccatttagc atgaaaatac atcgcatcat ccggtacttc
     2461 agtcaatgca taattaaatg tataaaaaat gtgcttaata aactcaggat gttcattcgt
     2521 taaagtaatc ttagcttttt ttcgaaatgg catctcaaaa tagcaattca tccctgatgt
     2581 tggattcact acgattggta aggaattcac ttcgcatctt gtcccaaacc cattacaaaa
     2641 aaaatctcct aatggacttt ctacagaagg agtggattca tcatcccaat aaatacgcaa
     2701 aattaaatca cgtaagacga aactcccagc gattgttttg tctgtcactg tcatccaaaa
     2761 atgacgtaaa attccagggc cttccatttc tgctaaagta attgtctctc cagcatttat
     2821 cggaacagaa ggtgaccctt ttctcccaat tcctagatga ctcgctgtca ttgccgcttt
     2881 ccctttttca ccatttttat tttctggtga aatagttcta ctttcctctc ccttgtggag
     2941 ccatatatta tttaaacaat tcattttatt cgtcctctat cctttcttca cttttttgat
     3001 tattcatata gatgatttgt tgttgtgcct ccgcaataat tgctccaata tctaaattat
     3061 catttcctaa ctgcaaagcg attgacattg ccaagggaag attcattcct gcaattacgt
     3121 gcactctgtc agaaatatag gtaaaaaact tttgatttac acttcctcca gccatgtcag
     3181 ttaaaacaat tatttcgtct gtagtatcca ctaactcttt aatttgttca tctatatcat
     3241 caatttcttc gacatatgca cataagctac taatttgatt atgcatacct gttaaaaact
     3301 ctagtgtctc tttaaaaccc tcagctagta ttgaatggct agcaatgatc aatcttctca
     3361 taattcaaat ccttttcctt tacgctaata ttccaaatgc agccataacc atcgcaaaaa
     3421 caataacgag tagaatcaaa atggtcatac tgattttttt cttactgagt agataatatg
     3481 ctgcagcagt aataattact ggaattaatg ttggtaaaat tttgtccaac attggttgga
     3541 ttttcatact aacttcacca aaagtgaatt taagtggcgt tgaaacactg ataactgaag
     3601 caattaatga tccaactact gttagcccca atactgaagc agcttcagtt aaataattaa
     3661 tcttttcacc gtattcagta acaatcttca ttccctgctt ataaccaaat tcaaagcctc
     3721 tcattcggaa aaagaagatt accatgatta aaatgaacca taaaaagatt cctacaggat
     3781 tattttctaa agccatataa gccgcaattg atcccatgat ggttgggatc atcgtcatca
     3841 aaagagagtc cccaatacca gcaattggac ccatcgtacc aactttaaag tcattaatcg
     3901 catcttttgc ttctaatcct tgcttttcct ccattgcaac acaagcacct aaaataatgg
     3961 ctgccagcca aggctgtgta ttgtaatact gaaagtgatt atttaaagct tcgatgtatt
     4021 catcatcatc tttgtataat tttcttaaaa taggtgacaa tgcgtaagtc acagatgcac
     4081 cttgctgtgt ttgataatta aaggtacaaa ctgacatcaa ccaacgatag ttcgctttcc
     4141 taagatcctt tttcgttact tgatattttt cgctcatttt attcatcctc cccaataaac
     4201 ccaacatcag aagctcccgc agaagcagtg atttgtgcat ctttttcagc tctatgatac
     4261 acattaaatg ctaaagcgaa tcctatgatc gccgctccaa taataggtac cttcaaaaaa
     4321 gcggctaaga aaaatccaac aagtaaataa ctaatgtatc gacctgtagg cataaagcgt
     4381 aacaacatcg caataccaac agcaggcaac atttttccgg ctaatgttag cccatccata
     4441 aaccaattag gtaaaacatc caaaacgtaa ttaacagcag actgtcctaa agtaacggca
     4501 ataaatactg gaattgctgc agataaacca aagaaaattg gccccaacca caaaacacta
     4561 ttcattttct taaattctcg tttattcgca tattcctgtg atttgcgaac aacaaaacca
     4621 tttaagattt tgacaattac atctagttgc acccctaaca tacctacagg tagtcctatt
     4681 gctaatccgg cttctactcc aagctggcta ccaaatgccg ttgcaataat tgcgcacgtt
     4741 ccatagtccg gtacagatga gccaccaaaa ccggcaactc ctaatgacat caattgaact
     4801 gtaccgccaa ctatcaaacc tgtttttata tcacccatca aaattcctgt gaataatccc
     4861 caaaaaactg cgtaatttac aaagatcatc ggaatcccat aataatcaac atgtttaata
     4921 aatgctaata tagtcagcat aataatttga aaaatagtaa aatgcattat ccttactcct
     4981 ttatcctaat tttgattgaa tttcctgtgc cttatccgcc ggaacaaatt gtgtaacaat
     5041 ttctacacca gaatccaaca attttttata cgtgctgata ttttcatctg atgcataggc
     5101 tctagaagat agctgtaatg cattatcttt aacaacagta ccgccaacaa ttagtcggtc
     5161 aattctaata ccgttatcta ctaaatgaag gattgtttcc ggctctttca cgatgacaaa
     5221 tactttttcc tgatcgtatt tccccttttt aaaattcgca atcgctgttt cttcagtgat
     5281 aattgataca gccattccag caggtctagc taaacgcata cttgattttt ggatgtcgtt
     5341 atttgcgaca gaatcatcga tcaccatcac tctgtttgct ttagacattg gtgcccattg
     5401 cgtgacaata atcccatgca ataaacggtt atcaactctc gacataacaa tactcataaa
     5461 tacttcctcc tttatctttc attttcttac aagcaattac catgccaaaa caaaaagaac
     5521 ttgataaatc aagctctttt tacataatca taaacgtatt taacctcatg aatcgggatg
     5581 tcgacactat aataatgttc aacgacactg aaagattgct taaaatgtgt ctgaaatttt
     5641 tgaaattcct cactcgtttc attgaaataa atttcatctt ccaaagtatt ttgttttact
     5701 aatcgttcaa ttaagcaact aatgtgaaca tataatccga aacacgtatt attatcaaat
     5761 gaaattccta gatcattttg taagatgtat atagcatctg aaacccgttc taataattta
     5821 gtcggattta atatggtcaa ttgctccatt aaattcgtta gactaaaatt cttcagaata
     5881 ttattgttca aaactttcag ttcattatca ttaaagtgtc tattcaattg attggagaat
     5941 ttacttatat tcattcctaa aatcatatct tcaattgaaa agaattctac atcttctaga
     6001 tgtggattta gtgttccaac aataaaaaga atattgttct ctttacgaag ctccgataaa
     6061 tagttttcat tcactaaaga gtaatagtca cacgtttcta tgacaagatc agtatccctt
     6121 ggaaagctat cttcaagtat cctttttaat ttaaacgaag ttcctaatcc gcttgcacaa
     6181 gaacagacaa tcatatcctt cttaatattt gatgattcat aaatataatc cacattttga
     6241 ttttctttta cttgttcaag aatctgttta agcggtaaat ctaataatat ctgatttgca
     6301 acatcaatag ccattcttgt tgaaacattg tttagtagtc cgaatttttt ttgagcgtct
     6361 agttccaaat ttagataaat tttctccagt gaacccatat caacaagtaa aataatttct
     6421 tcatatccgg tacactcttg taagtatcta ttaattttgt ctgcaatctc actacttgaa
     6481 gtcattagtg gcatatcaat ggcgtcgtat atatatctct caacgatcct attagcagta
     6541 cttgctatac aggttgccgt tagatctcca tgagccaaaa tcagtgctaa acgtttttgc
     6601 tgaaattctt tactacacat taacttaaat aaaataacca aaacattttt ttgaattttt
     6661 gttaaataaa tatcccctat ttgaataaaa cgattgacta attgaacttc tcgactacac
     6721 caactattaa tatcattgat gaaggaatat aatttatcgt ccgatgttaa ttggatttga
     6781 taaatccaag aattcaaaaa gatagacgtt atttgaagat aataatagtc aaattctatt
     6841 tgaaaatctc tctctatttc cttaacatac tgtctaaatc gaatctcttg ataggtggga
     6901 tatgttaaag ctgaattatt ttggatatca acattctttt gtctaaaata tctatcatat
     6961 ttctcaaaaa gctgctgctt atcagtgtct ttcttccacg tttgataaag atactctata
     7021 agttcgttga tattaaaaac ttcgtctact ttttccaaat catccatttg aatatatttt
     7081 aattcattct gtcttttcat aaccttcatg ccgttgaaat tcacccattg tggtaaatga
     7141 tacatctgaa tataaagttc ttccaaatca tctaagaaat aaaaggcatt ggcacaggtc
     7201 aattgaatga tatttttcaa attgccaaca ttttcactaa agcgatagtt taataaaaaa
     7261 tctaataatt gttgagatat cagtattttc tttgctagtc tttcttcttc tgatttaaaa
     7321 aatatcttga ttaactgttt tctttcataa agagaacgac tgtctaatgt tggaagtgtt
     7381 agctttacag gtattcttct caacaaagtt gataatagtt tatcctcagg tggttctgat
     7441 gttgcaaata taatccagca acttgatgtg taccagtttt cattatcacc catccgatga
     7501 aatttcctcg tatccataaa ctgaaaaagt ttctcctgac attcattgct caatgcatga
     7561 atttcatcta aaaataagac tccattatta gctaattctg ctaaaccctt tcttccatct
     7621 tcggctcctg tatatgcacc ttttacatgc ccaaaaaaat tggacaagaa aagttctgga
     7681 ttattggcat attcactaca attaactgaa atgaaagatg ccttctcttc tatataatta
     7741 ttggccaaca aatccttata cacataattt gctaaatagc tttttccagt tccagtctcg
     7801 ccatgaagta aaatgggtaa gccatttggc ggatagtaga ttgctgcttt aatttgagca
     7861 attacatttc gcagacttcc ttgacttccg ataacccttt ctattccaac cctaaaagag
     7921 ctatcatacc atttttccgc ttcctctgaa gagctaaaaa tttgttgatt tggaactttc
     7981 tccggtaaag aaaagaagat tactggacga gtattgattt ttccaaattc tccatctttt
     8041 acgaattcat tcaaatattg agagacaata ttacgactaa catttaattt ttcagatatt
     8101 ttctgtgctg tattgttccc ccatttattt ttaatctcag agttctctat gacataattg
     8161 acaatgcgtt cttttaattc ttccatcaca gcacctcctc gacactattc gaattttgcc
     8221 gacagtattt ttccataaat atgacttgct aaaaaggctt tgtttagatc attcaattgg
     8281 aacgttgctt gttttgcttg atccgctaag acgttgaaaa gatactctgg cgaaatcgca
     8341 taccccaaaa tatcaacgat tgttgcaatc agatcttctt ttgattcttc atctgaaagt
     8401 aattcacgat acaacatcgt ttgtttgctt actgtgtcat attcctctaa ggattcatct
     8461 gctaaaagaa ccacttgttc caacaaacgg tcggataaat atttatagaa aatcagcccc
     8521 aataaataat ttttatattc tgacgcatcc attttactac ggagattatc tgccgcacta
     8581 aataacttct gatttaattc tgcacccaaa aagatacact cctatctctt atttaaagtt
     8641 gctatatacc ctcttatttt agcatactca gttttagaaa aagcaaaaaa ataaagaaac
     8701 ttattcttga taacaagcta cgtgacgtat atccctaaca caaaaatagc catatccttt
     8761 ggtttggata cggctatttg atattactca ttcaattatc aaatcacagt aggtgtgaat
     8821 ctcatcggga tcggcaatat ctacttctga ttaggatcaa aggtgatttt ctcaataaag
     8881 gtgtgataca aagctttaac ttgcttcttc tcactctgga taagaaagca atccaaacta
     8941 gtcaatatct gttggacatc tttgacttga atcggttccc cttgttgttg aaagatccct
     9001 aagatttcat tctctcgaat ttgtaactgt cttcgttttt cagtcaattc atttaaacga
     9061 tcttttaaca tcggaaacaa ttcgggacta tcttccaaag cagcttcctg tttttgctct
     9121 aatagttaga ttttgcgtac cattttcttc ttttattttc cttcgattgc tgattttaat
     9181 atagtctaaa ggaaatttat atttattgta ttctttataa cttcaagaga acttcgtttg
     9241 agtattactg tattttttta attccataag aaaattagtt attacctgcc acctaatcag
     9301 caaatctgaa cctgagtcaa tataattaat attattttcc atctccaaca ttgtaagtac
     9361 tgttggtgtt aatataggga ctttgtacaa ttgtattttt ttgattttat catctgcaca
     9421 aaggagattt tttaaatcta atccttcttt tatttcaaaa gttgaatttt ttaaatctat
     9481 aagagtaaaa ccaattcctt catactgaat ttctgaaaat cgaatttttc tcccagttga
     9541 tagatcaagg ggcagaaaac cttttattac attgtgagtg tcaccatcat ttacaatgtc
     9601 attaggaatt ttgattttag aattctgtgg agcacctatt ggaagataat aattcattat
     9661 aattttgtag tcttcatgag gaaataaagt acttttagga ttgctaagat attctcgtac
     9721 cgaatctaag aatggcttat ccattgaatc ttcatcccca tttaaactaa aaaacattcc
     9781 tacaattgct tttgcaactc taagtgaatt cattttcttt attgaatgtg ttaaatcaga
     9841 cttaccattc tttaaacttg tattgttata aaggaaattt ttattgtctt ctgcaaatct
     9901 aatatattcc tcaacatact cactaccaaa aaaactatta catttactac ataacgtatt
     9961 ggaatggcaa cagttttttt gacaaatttt ataaggtgca gaacttcttt ccgtatgcta
    10021 ttccgattgt ccttgacaat gagcctcctc ggatgtgacg atcttcggca ggagctaaca
    10081 gttaaagtta gactgcctcg ctagcgttag cacatccgag ctcattatca aggattcgct
    10141 gcgccaat
//
LOCUS       DACDIH010000086        10074 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDIH010000086 DACDIH010000000
VERSION     DACDIH010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.086"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(460..1308)
                     /locus_tag="IV281_002374"
     CDS             complement(460..1308)
                     /locus_tag="IV281_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8781345.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1472..2374
                     /gene="gnd"
                     /locus_tag="IV281_002375"
     CDS             1472..2374
                     /gene="gnd"
                     /locus_tag="IV281_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP8781346.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2376..3377
                     /locus_tag="IV281_002376"
     CDS             2376..3377
                     /locus_tag="IV281_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8781347.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3374..3658
                     /locus_tag="IV281_002377"
     CDS             3374..3658
                     /locus_tag="IV281_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8781348.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3691..4488
                     /locus_tag="IV281_002378"
     CDS             3691..4488
                     /locus_tag="IV281_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8781349.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4502..5335
                     /locus_tag="IV281_002379"
     CDS             4502..5335
                     /locus_tag="IV281_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8781350.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5353..6210
                     /locus_tag="IV281_002380"
     CDS             5353..6210
                     /locus_tag="IV281_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8781351.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6232..6741
                     /locus_tag="IV281_002381"
     CDS             6232..6741
                     /locus_tag="IV281_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8781352.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6778..7179
                     /locus_tag="IV281_002382"
     CDS             6778..7179
                     /locus_tag="IV281_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781353.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7291..7644
                     /locus_tag="IV281_002383"
     CDS             7291..7644
                     /locus_tag="IV281_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781354.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7678..8121
                     /locus_tag="IV281_002384"
     CDS             7678..8121
                     /locus_tag="IV281_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8781355.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            8138..9223
                     /gene="uxuA"
                     /locus_tag="IV281_002385"
     CDS             8138..9223
                     /gene="uxuA"
                     /locus_tag="IV281_002385"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8781356.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9216..9929
                     /locus_tag="IV281_002386"
     CDS             9216..9929
                     /locus_tag="IV281_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8781357.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         3384 a         1588 c         2194 g         2908 t
ORIGIN      
        1 atagaaaata tgggaatata atagaaaaga tttaggatac ccccaaaaga atttccccaa
       61 aaattcgatg tatcctaaac ttttttattt tacataacaa tcatatcaac tgtgaaataa
      121 aattacaaat gataacctaa aatgaggcca aaagccagtg ttatcaagga aatacttaat
      181 tcaccaaaat tttttttaat tttttagaaa ataagaaaca aaaataataa aaaaaagaaa
      241 agataaattt tttactactg tatactaact ttgttatcgc atctaacaag taactcgaat
      301 aataccaata caaccttctc caaagatgcg atgacatacg ttctaaccca ccgactattg
      361 atgttctgta ccacccttgc agaaccaaaa aagccactac caaatatttg gtagtggctt
      421 tttttgtttt atggattttt cgctttttct gttatggtgc tattctttga tttcagaaat
      481 agtatgtcga actttagcga tagaacgacg tgattctttt tcgttgtaaa acatcaaaat
      541 cacatataga gagtccaaaa tgcttagttg agaaatacgg gaagctaaag cttcagatcg
      601 gaattctgtt tcttcagaaa tagaaataaa aacgacatcg ccaagtttag caagggaaga
      661 attagcttgg ctagtaataa cgattgtttt tgctccagtt tttcttacgg tttcagcaat
      721 atggattgtt tcttttgatt gccctgtgtg agaaatcaaa acagcacagt ctttttccgt
      781 caataaagaa gcttccatca attgaatatg gtaatccgtg ctgtgagaag ttgcgatagg
      841 ggaacgcaaa aatttatgat aagcgtcagt tgctacaatc tctgaaccgc caacaccgaa
      901 aaaatagaca cgtttagatt cattgataat agtagccgct ttttttagat ccttttgttg
      961 taacaacttt tttgtgtcat ttaaagttga aatactagac tcgaacacct tttgcgccat
     1021 agtgagctca gtatctgttt tctgaatatt ttcgtggata gaaatagcag atagatcatt
     1081 ttcttggatc aacatggcca ttttaaaatc tttaaatcca ttataaccaa gtttttttgt
     1141 aaattgaaaa aaagtagagt cagcaatgcc cagttcactt gctaaattgc taataggact
     1201 atgacttact ttgcttggat tttctaaaat ataatcggcg atagcctgtt cttttgggct
     1261 gaagtcgttg tacattgttt tgatttgtag tcttatggag acgctcatga gaattcctca
     1321 ttttcataat aagattatca ttttttccgt tctcattaat gttaaagata caaggagagt
     1381 aggaaaaaat caaacaaaaa atatttttgt aaaatctatt tacaaaaaaa tttttttgta
     1441 taatgagggt ataagaaatg gaggcgctat catggaaatt ggtgtaattg gtttgggaaa
     1501 aatgggattg aatttagctt taaacttaaa agagcaccat cacaaagtac aaggactcga
     1561 catttcaagt acagctgtta gtcaggcaag agaaaatgac attcctgctt atcaagagat
     1621 ctctgaattt ctagctactt ttgagcagaa aaaaatcatc tggttaatgc ttcctgctgg
     1681 agagataacc gaaaacatgt taagaaaact ttttccaaaa ctcggaccag gagacattat
     1741 tattgaaggc ggaaactctt attataaaga ttcaatccgt cgagcagaag agtttcagaa
     1801 aaatgaaatc ggttacttgg attgtgggac ttctggagga atcgaaggag cacgacatca
     1861 agcctgctta atgattggag gagataaaga aacttttcta tccgtggaac aattattcaa
     1921 agatgtagca attgaaagag gatatctgta tgctggtgca tccggaagcg gacacttctt
     1981 gaaaatgatc cataatggaa tcgaatatgg aatgatgcaa gcaatcggtg aaggattcca
     2041 acttgtacaa aaaagtgagt atgcttttga cttggaacaa gtcgcacgtg tttggaatca
     2101 tggttcggtg attcgcggct ggctgatgga aatcatggaa gatcagttaa acgaacatcc
     2161 caatttaagt aattatcggg gaatcgtttc tgcatcaggt gaagcaaaat ggacggttga
     2221 aacagcgcta gaaatggctg ttccagttcc agtaattgca actagtttgt tcatgaggaa
     2281 tctttcacag gaagaagata gtttttcagc aaaagtagtt tcagcactaa gaaatgggtt
     2341 tggcggacat gaaatagttg aaaagggtga gtaaaatgcc agattatcat atagcaatcg
     2401 ttaattccag cagtttcgga agagtctttc cacaccactt ggaaagatta aagtcagttg
     2461 gagacgttca tttctttact gttgatcaag agattccagg aaaagaacta gcggagttac
     2521 ttaaaggatt caatatcatt attgcaagtg ttacgccatt ttttcgacga gatttttttg
     2581 aaaacaaaga tgaactgcta ttgatttccc gacatggtat tggttacaac aatattgatt
     2641 taaaagcagc aaaagaacac cagacaatcg tatctatcat acctgcttta gttgaacgaa
     2701 atgctgtagc agaaaataat ataactaatt tgttgtccat gcttcgacgc acgacacaag
     2761 cacaagcaag agtcaaggac gatcgatggg aagatcgggc ggagttcgtc ggtaggacgc
     2821 tgtttaacaa aacagttggt gtgattggtg caggaaatac cggaagcgga gtaatcgaaa
     2881 ttttgcgtaa cggttttcgc tgtgatgttc tagcttatga tccttacaaa tcagatttat
     2941 acatcgaaag ctttggtgca aaaaaagtag aacttcaaga attactggaa caatcagata
     3001 tcatttgttt atgtgcgaat ttgacagaag agaactatca catgatttct gataaagaag
     3061 tcgatcagat gaaagaaaat gtttatctat ctaatagtgc aagaggcgct ctagttgatg
     3121 aagaagcgat gattaatggt ctgaaaagcg gaaagatcgc aggatatgcc acagatgttt
     3181 tagaagaaga accgggacgt caagatcatc cgtatttagc gtttgaacaa gtagtgatta
     3241 cgccacatat atcggcctac acaagggaat gtttagaagc aatgggtgaa aaatgtgtca
     3301 gagatgtcga acagatcagt cagcaccttc ttcctgaacg ttctgtccag acaaagagca
     3361 actatataaa aggatgatga agaaaatggt gaacggttta aaagtgaaaa caggccctca
     3421 attttattta tatgaagaag gtggtatttc aaaagtttct gatctcttga aatcatacgg
     3481 cgcgaaaaga gtactggtca cccacggaac ggtatcatgg gaaaaggcgc tacctaaatt
     3541 agtatttttg aatgacgaaa cgattcaatt tttttatcat cgttattctg gagaatgtag
     3601 ttatgctgaa gccagacgaa tagctacaat catcaaaaaa atgaaatcga tttcgtagtt
     3661 ggcgttggcg gaggaaaact gaccgatcta gtgggttata gtgccaatct tgcaagaatc
     3721 gatttcggag tcatccctac tttagctagt aattgtgctc catggacacc tcttgctgtc
     3781 atgtataagg agaatggaca agcagaaggg aaaacagaac acttttacag acaagctgct
     3841 ttttttataa cagatcccca attagtgatt gattcccctg ttccatattt cattgccggg
     3901 ttagcagata cactagcaaa atggtatgaa tctgagctca tcttagaaca agattttttg
     3961 caagatgaat catttttgaa gctagcacaa gatactgcga agatctgcaa agaagagatt
     4021 ttgaatcatt ctgctaaagc aatccaagat atggcacaaa gaaaactgac atcggagttc
     4081 aaacagcttt cagagattat ctttgctgtt gctggtttag ttggaggatt aggagataaa
     4141 tacgcaagaa atgtggcagc acatgctatg catgacgcta tcagtaaata tattccggaa
     4201 agccatcgtt ttctacatgg agaaaaagta gcgtatggga ttttctatca actggcacta
     4261 gaaggtcgct gggaaaaaat agacgagtta tatcctttgt atcgggaatt agaactgccg
     4321 gtttctctag aacaaatggg attatttccg aaagataaac agataataga taaaatggct
     4381 gaatttattg attcgaaaga gaaagtgcat ttgattccta tagaggtaac acagaaatca
     4441 ctgatacagt caatgatgga actggaaggt tatatcagaa agatctgaag gaggaggaca
     4501 catggaaaca ttaagtgtag tacaaagttt gttgattgct ttatgggtag ccgcggttat
     4561 gtcccgctgg ttaggtggag gagccacgtt gaccttacgt ttttctcctt tgatgactgg
     4621 attagtcgtc gggttgatca tgggggaagt tgcacaagcg atgattgtta cagccgcttt
     4681 gcaaatgatt tatatggggg ttttttctcc aggaggatca atgccagctg aaccatctat
     4741 tgcagcagct atcgcagtac cagtcgcatt attaggggat ctgtcgccag aagcagcgat
     4801 tgccgttgcg gtgccagtcg gattgctcgg aagttacttg tatcagtttc gttttttcat
     4861 caatactttt ttaggtaaat atacagacaa agctgtagaa gatttgaata caaaagctat
     4921 ggcccgatca attattcttt atccaacggt tgcctcattt ttattattcg ttccgcttgt
     4981 tttcattgct ttatatttag gggcgccatt gatcgctgat gtaatcaaag ctttagaggg
     5041 aacagtcgtt atccacattc ttgaagtagt aggtggaggg ttagcggcaa tcggtatcgc
     5101 gacaactgta tacgttatcg gtcgaaaaga ttatctggtc tttttcttcc ttgcttattt
     5161 tatgagtgtt gttttgaaat ctttagatat tacgatggtt acttatgcca tttttgggat
     5221 tattatcgct ttgatctttg tccagtcgca aaaagggaaa caagtacagc ctgccacaag
     5281 caataacaat tcagcatctt ttgatgacga agatgactat gatgacggat tttaatttag
     5341 gaggaatggg aaatggctga aataatggat aatcaaacag gtgctcctga agaaatcacg
     5401 aaaaaagatg taacaaaagc ctatctgaga tggcattttg caaacgaaat tcctcactct
     5461 tttgaacgtt acttggcgcc atcactacta tacggaatga tgccaatctt gaaaaaactt
     5521 tataaagatg aagactcttt aaaagcagca tataaacggc agttactgtt cttcaacact
     5581 caattaagct ggggtggcgg tgtgattact ggcctaatgt cttctatgga acagcaaaga
     5641 gcagaagaag agtatgaaga aaaagagatc ttgatgcagg atgacttgat gtataacacg
     5701 aaagcgggat tgatgggggc actagcagga atcggagatg caatcgattc aggaaccgtc
     5761 caatatattt ttatcgcaat cgcggtacct tgggcacaac agggaagtcc tttaggcgca
     5821 ctttttcctt ttatctgctt tgggttgtat caggttttac taggggtatt tttcgcaaga
     5881 caagctttca ctatgggccg taatgctaca ggcctaaagc aaaacactgg tgttcagaca
     5941 gcgattgaaa tgctatcagt tttagggttg tttatgatgg gggtgcttgc gggaaactat
     6001 gtaaaagtag aatcttcatt gaaatttgcg atatccggac gtgagtttgt tatccaggaa
     6061 attcttgatc aaatcgtgcc agggatatta cctttagctg tcgtaatggg tgtgtacttg
     6121 ttctatacga aaaaaggatt gaaagtcaca caagcattat tatggctgac tggaatatta
     6181 attgtgctag caggaatcgg tattttataa aaagaaggga acgtgaataa gatgggtgta
     6241 gtcaatttag cgcgtgtgga tgagcgtttg atccatgggc aagtaatggt aacgttatct
     6301 caaaaaagtg gagtgaattc catttttgtt gtagacgaag ttgtagcaaa agataaattt
     6361 atgagagatc tttacaaaaa tgcaggaagc cgtacaggac aaaaaacaat tgtcattaca
     6421 ccagagaaag ctaaattcta ttgggatgaa tatcaattta aagaatacaa ttgtattttg
     6481 atcgcaaaaa cagtccaaac gatttacgac ttagtaaaac acggaatccc catggaagaa
     6541 ttgaatattg gagggattgc tcaaaaaaat cctgatacag atcttttagt gacgaaatct
     6601 gtttatttaa ataaatcaga tgctgaaaaa ttgaaagaaa tgaatgaaca atatggcgta
     6661 aagaatattt attttcaagc cactccatct gcacctaaaa caggattaaa agatgtattg
     6721 aacaagtttg atttgtcata aacaaatgct tagaaaaaag ataaagaggg cgaaaaaatg
     6781 aagaagaacg agctgtttcg tgattgggaa tttcgttatc gctatattta ccgtaagcgc
     6841 cgtacaaaaa agagtaaaca gcgttttctt tcagcattag tatcagatat ttacagtatg
     6901 agaacagatg tgacagtcat tgcttacgat acgctagcct accgatcaaa aaatatttat
     6961 gttggagata tcgaaaaagc agagaaagtt atctgtactt attacgatac acccgttcat
     7021 gcattaggaa gttatttcat gtttgattgg aaagaccaaa ggaaaaaaac aatctactct
     7081 atattattgt ccttcatttt gttgttcagt ttaggttggt ggggaatgat gatatataac
     7141 aaaaatcctc atcacgtatt cgatctgcta tccgtataaa caggtatcac tgtactagca
     7201 tttgggagct actttttctt attgggaaaa gcagctcgcg gatggcctag cagacaaact
     7261 tttattcgaa atacttcatc catattaacg atgctagaaa tgatccgtac gattgatgat
     7321 ccgagtgtag cgtatgcgtt tgttgatgaa ggctgttatg gagaaaaggg cttagacagc
     7381 gtgagatcgg gtatgaaaaa agaggctatt cttttttatc tagactctgt tggggcagat
     7441 acaccgctac agttttcggg aaattatttt tcaaataaag aacagtggct gaaacaagtt
     7501 gataagttga aagaaaaaaa cgtcaattat atttttagtg caagaaaaaa gcaagcgcag
     7561 ttcttctatt tgaccaaaac agatttacga gggaaaacat ttaattggca aaatgccaat
     7621 cagattattg ctctttttcg ataagtaaaa agtgcaaaac aaggagtgag aagactaatg
     7681 ttggaaatca tgatcgttac acatggtaag ttgagcgaag gactgaaaga tgcggcggaa
     7741 gtaattgtcg ggacatcgag ccatattgaa acaatgtgct tagagtctgg ggaggacatc
     7801 caaacattcg gagagaaggt caaagaaaaa ttaactatga tcaacacagc agaacccatg
     7861 ctagtatttg ttgatctaat tagcgcaagt ccgtacaatc aagcgctctt ggcagtcaat
     7921 gaactgactg aagaaaagaa agcagccact tatgtcatag ggggcgtgaa tctgccaatg
     7981 ttgttagaag caatcaatca tcagatgctt cacacaccga tagaagaagc ggtggaacaa
     8041 attgtcaaac aaggaaaaga taatttaact gtttggcatg cttcagcgga agaaaaaaaa
     8101 ttagatgagg acgattttta gaaggaaggc tgaaaagatg aagtggggat ttagatggta
     8161 tggtgaaaaa ggggattcga tcccattaaa atatgttcgc caaataccgg gaataaatgg
     8221 gataattgga actttgctga ctaaaatgcc tggagatgtt tgggagattt cagaaattga
     8281 ggagttgaaa gcatctgttg agagccaagg actggaactg cttggaattg aaagtgtagc
     8341 gatccacgat gcaatcaagg cagggacaga tgaacgagat tactatattg ataactatat
     8401 acgaacaatc aaaaacttgt ctcgatgtgg ggttcgattg atttgttata gcttcaaacc
     8461 aatttttgga tgggcgaaaa ctgatttgtt taatgaaaac aacgatggaa gctattctct
     8521 tttatacgat cagaaaatcg tcgatcagat ggagccagga gaaatgtatc aattgataca
     8581 cagccagtca aagggattca aattgccggg ttgggaagaa gaacggttga aaaaattcgc
     8641 tcagctggaa aaactgtatg aaggaatgac cgaagaagaa ttatttgaaa atctgactta
     8701 ttttttagaa aaagtgattc cagtgtgcga aaaagcagat gtaaaaatgg ctatccatcc
     8761 agatgatccg ccatgggaac tttttggttt tcctagagtg acgaaaaatc ttggcgactt
     8821 gaagaaaatt ctaaatgcag tagattctcc atataatgga ctaacgctgt gcacaggatc
     8881 tttaggggca aatccggaaa acgatttagt tgaaatcatc catgaggtag gttcgcgcat
     8941 ccattttgtc cacttcagaa acgtggagtt tttaggagaa agaaagttca aagaaacggc
     9001 acatccctct acagaaggct cccttgatat gtatcatatt atgaaagcgt tggtgcaaga
     9061 agggttcgac ggtcctatac gccctgatca tggtagaacc gtgtggggtg aagtggctat
     9121 gcctggatat ggcctgtatg atcgtgcaat gggactcact tacatgcaag gtctatatga
     9181 ggcgataagt aaggaaaaac cactgggaga gcattatgaa taaaatcaag ctcttcatct
     9241 ttgatatgga tggcctttta ttagaaactg gacgtttagc ttatcgtgct tatgtaaaat
     9301 cagcacaaaa gtatgattat gaaatgcgta aggaagtcta ttatttacta actggccaaa
     9361 cagaaatggc gataagagaa caaatgggca ttttgtacgg agaagatgtt ccatatatca
     9421 aatggcgaga agcaatcaat caatataaag aaaaaatcgt gaaagaagat aagagagtat
     9481 atacgaaaaa aggagcagaa gaaattttat cttttgcaaa agaaagagga atccatacaa
     9541 tcgtggcttc gtctaataca cgggtaaaag tagaaatgta tttaaaaatg gaaaatctat
     9601 atgccttgtt tgatcaaatc atttcagggg atgatgtaaa aaaaggaaag ccggaaccag
     9661 agatcttttt gaaagcttgt tcaaaaatga atattcctcc aagtgaagca cttgtatttg
     9721 aagactcgat tgcagggatc gaagcagcaa ggcgagcggg cattttgtct tttctcatca
     9781 aagatcattt gatagggttg cccgatcata atgggaagca taagttgaaa gtagatgtaa
     9841 gtttatatga atccaataaa gcagactatg aattttatga cctgcatcaa gcaaaacgat
     9901 ttttagaaag taaagggttg tatctctaat aaagaggact attttctagt cctctttatt
     9961 tattgtcctg cacgactgag ggaacagatg aaaaagaagt aattttttat tttgaagtat
    10021 ctaccttatt tgtagtgaac aatttagaac aaacacaaaa cgcatctact gtta
//
LOCUS       DACDIH010000087         9976 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDIH010000087 DACDIH010000000
VERSION     DACDIH010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.087"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(233..1015)
                     /locus_tag="IV281_002387"
     CDS             complement(233..1015)
                     /locus_tag="IV281_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781358.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1080..1916)
                     /locus_tag="IV281_002388"
     CDS             complement(1080..1916)
                     /locus_tag="IV281_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8781359.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1934..3343)
                     /locus_tag="IV281_002389"
     CDS             complement(1934..3343)
                     /locus_tag="IV281_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8781360.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3357..5180)
                     /locus_tag="IV281_002390"
     CDS             complement(3357..5180)
                     /locus_tag="IV281_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8781361.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6023..6349)
                     /locus_tag="IV281_002391"
     CDS             complement(6023..6349)
                     /locus_tag="IV281_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781362.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6395..6757)
                     /locus_tag="IV281_002392"
     CDS             complement(6395..6757)
                     /locus_tag="IV281_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP8781363.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6794..7009)
                     /locus_tag="IV281_002393"
     CDS             complement(6794..7009)
                     /locus_tag="IV281_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8781364.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7136..7471
                     /locus_tag="IV281_002394"
     CDS             7136..7471
                     /locus_tag="IV281_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8781365.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7547..9544)
                     /locus_tag="IV281_002395"
     CDS             complement(7547..9544)
                     /locus_tag="IV281_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781366.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9642..9917)
                     /locus_tag="IV281_002396"
     CDS             complement(9642..9917)
                     /locus_tag="IV281_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8781367.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
BASE COUNT         3072 a         2085 c         1478 g         3341 t
ORIGIN      
        1 aaatttctta aaaatttttt cactaaaatc actacctgaa acagtctaaa acacttatat
       61 atcaacgttt aatgaaacta tctccgtttc atttcagata caaataaata tagtcgggct
      121 ccaaatccga aaaaaaatac ccccagtagc ttatcctaag ctactggaga tgtataagta
      181 cggtcatata acaacggggt agggtaaata aatgaaaaag atggaagaat cattattcaa
      241 taactttttt aaatacttcg ttgtaatcag ttacctgtcc gttcttaaat accacagaat
      301 aatctttttt cccattttca agtaaccgaa gtggaatgtc tttaccaact tgctctgtca
      361 ctgcattact gatacctgtt agattagcta ataaatctga taaagattga gcatctgtag
      421 aaatctctgt atcagtattt atcgtgattc ctttatcttc atttacaagc ttccaagtag
      481 gtggtatagc tgtgtatgtt tcgatgaatg aatcaattct ttcatctatc gtcttcccag
      541 tatctacttc tgtctcatta ctcttagatg aatcgattct ttcatccatc gtctgctccg
      601 taactgcttc tgtctcatta ttctcaccgg aaactctctt cacacccaaa cttatatatt
      661 gtccatcttt agtgtgcata gttaaaattc ccacgctttt actaataaat tcactataac
      721 cagttctttc aatagagact tcaatgtatt tcacactatc atactcaaaa gaaaaataga
      781 catgtaaaag cattttgttg tttacatcta cataataatc ctttgactct acttgagacc
      841 ttcctatacg gaatacagct ttactgtctg atatctctat accagcatcc ttattattaa
      901 tctgccaatc tccaagtagc tcttctggca agctagtagt ttcttgtgaa cactttacca
      961 cttcactggt agaggtcgaa acattcacct ccgcactgtt agaagaattt tccaaggaat
     1021 caggagcctt agcacaacca acaagtcttt atatattgtg cagccgatat tttcttcatt
     1081 cagtacaccc ttgaaatatg cagtgttaga taaagcagtt cgtcctgatt tgctgaggca
     1141 cccatctctt cttgaacgta ctccttgatt ttcaaggccg cccgatactc tttcggaaaa
     1201 cgctgcttca ctgaagccag caaaaaatca tccttctccg gcaactcttc cttcttaatc
     1261 attcgggcaa taaaaaactg taaatgcgtc acaaatctta agtaaagcat cgaatcttta
     1321 tcaacctttt tttcgggaaa cagatatcct agcatagcca agatatcctt tagtgctctt
     1381 accgagagca tggtttcttc catatttgct tgttcacttt gggcatttac aatgtgaaac
     1441 gcaatgtttc cagcctcctc ttctggcaac tgaacattca tttttttgtt gatcaattgg
     1501 atagcatagc agccgatctc aaattctttt gggtaatact tttgaatttc aatcagcagg
     1561 cgattttgaa aaacaatgcc tttttgactg cgttcaatgg catgcttcaa gtgatcaacc
     1621 aaggtaaaaa atagactttt attcagctta aagtcaaact tggcgttggc ataatcgata
     1681 attcgcttga cgcaaataat tagatcatcc ggagcagcct caaacagttt aatgtaatcc
     1741 ttttttagag catcagaaag tgaatctaat ttaaatactt tttggatttt gtcagccgga
     1801 accaaatcgc ctttctttag ttgaaatcca atccctttcc ccatcaaaat tacttctttg
     1861 ccttgcttat ctaagcaaag aattaaactg ttgtttaacg ttttaattac tctcattaga
     1921 ttcaccgttc tctctaagct aagccatttc ttgcgattac tttttgatac caaaagaagc
     1981 tttctttttt tattcgtttt aattctttaa gattctcatc ggttcggtca acatatataa
     2041 aaccataacg cttattgaag ccttgatggg tactaacaac atcaatcgct gaccaaggta
     2101 aataaccaaa aacatctaca ccatctgtta aagctgcttg aatagccaga atatgtttgg
     2161 ctagataatc aattcgatcc tggtcattaa ttcgtctatc ttcctcaact atgtcgggtg
     2221 ccccatagcc attttcagta attaaaatag gtaaatcata gcgatcgtac aatttcctca
     2281 atgttaatcg tagtccagta gggtcaacta cccagccata tggcgtttta tctacataag
     2341 gattttcagc tgcccggtag acaccttctt ctcccaacat gatctgttga tcccccgctc
     2401 tagcggaaac atctgatccg tcatttttgc tggcagcgat ggtggcagtt gaataataat
     2461 taatcgcaat aaaatcgggt ttaccggagg ataaaattgc ctgatcctct gcctcaattt
     2521 taggatataa tccacgatca ttcaaatagt tttcaaacaa ataattgtat tttccccgaa
     2581 ctgctacatc taagaaagac cagcctctca atgtttccca attatgggct gcaattgcat
     2641 cttctggact ggaggtagct tgatacatag ccgttaaatt gattgctgga ccgatttttc
     2701 ctttaagatt caactgatga tacaagtgaa ttactctagc ttgagctaat aaaacatggt
     2761 gattaatttg aaagagttct ttttttgaag gcagctctcc acttggtggc aaaccaatcg
     2821 ctccaggatg aaggatcatc gtgttttgct cattaattgt cagccaatag ttcaccttgt
     2881 caccatactc agtaagcaat acctgtacat agtccacaaa ataatcgata gttgctcgat
     2941 ttaaccaacc gccctgctcc tctagaaaat aaggtaaatc gaaatggtac atggtgacaa
     3001 tcggttcgat tccctgattc aggcattcat caattacttg atgataaaaa gaaattccct
     3061 tttgattggt tttattattt tcaatatcca taattcgcgt ccaagcgatg gaaaagcgaa
     3121 aagcttttaa gcctaattct ttaaacagct gtatatcttc tttatagtga tggtaaaaat
     3181 ccgaggcaac ttcaaaatta gctacccctt ctgtatgctg atacttgtca ataatagacg
     3241 gttgtttccc atcctcctga taggcaccct ctgcctggta ggcactaatg gaggcacccc
     3301 ataaaaactt ctctggaaat gctgaaagct gtttgtattg cacaaaaatc ctctccttaa
     3361 ctaatttgca tcaatggttc gtgttccttt aagcttccgc ttgcttcact aattcctgcg
     3421 taattttcac tgttggtcac taccacaatc actgtatcat cgtagccagc gtttctaatg
     3481 gcagcgaaat cggcgttgat taataattgt cctgctgaca cctgatctcc tgcttttaca
     3541 aatacttcaa agggttcccc ttttaaattc acggtgtcga tccccacatg gatcaataat
     3601 tcaatacctt ctttagaacg tagaccaatc gcatgtttag attctaaaat agtaatgact
     3661 tctccctcaa caggtgaata aatgctgttt tcttctggat gaatggctac gccctttccc
     3721 aatagtccct tagaaaagac agcatcgttc acttcagaaa taggacacac ggttcctttt
     3781 accggatttt caacaatgac tgcttcggct attgccgttt gctctgattc atcagaagta
     3841 tcgtcaaatc caattagata agcaatgata aaggttgcaa caaaggcgat tacacacgta
     3901 ataattgcgt gaacaatatt cattggattt tctccaataa aggctggtaa ggctgctaag
     3961 ccaggggaaa caaaggcata tctgactaaa ccgctcaagc cagcatacaa accagcaatt
     4021 gcaccaccaa tcatggaagc aactaatggt tttttcagct tcaaggttac tccatagagt
     4081 gaaggctcag taattcccat taaagcagtg atcccagtgg acacagctag ctgcttcaac
     4141 tctttgtttt tagttttcgc ggctacagca aaggtagcac ctgattgagc aatattagaa
     4201 gccagcattc ctggcccatt gatcatttca gaacccgtac tgctcaattg catagtagcg
     4261 attggcgtca ttgcccaagc tgtacccgta accaccaaaa atggctgcaa accacccatc
     4321 aaaaatggaa ttaaccaagc tgctttgccg ttaatccatt cagcccccat tgctataaga
     4381 tcgttcagca acgttccaaa cggtccaatg acaaccaacg ctaaaggccc cataattaaa
     4441 aataccagca tcggcttagt aaagaattta ataaacgaag gcgaaattcg atccgcaaag
     4501 ggctctacat aggacatgat ccaaacagta aagataatcg gtaaaactga tcccgcataa
     4561 tctgcagcta ataccggaat tcctagaaaa gaaacggcat cgccagtaga aaaaagctgt
     4621 gaaatatttg gatgaagcaa aatgccagca acagtaatcg ccaaaattgg gttgacatca
     4681 aactttatgg ctgccccata ggctaatagt actggcataa aataaaaggc tgcatcagca
     4741 atagtattca aaatgatgta agtttgacta ctgtctgata gtacaccagt caaagtcaag
     4801 acggccaaaa tggcttttac catcccggca cctgttatag ctggaatcat cggtgtaaag
     4861 gtggtagaaa tgacactaat caatcttgaa ataatacttt cttgattgtc tgaagagggg
     4921 ccctttttaa ttgaagttcc cacttccttt tgaatttcct tgaaggctgt ttggacttca
     4981 ttgccaatca ccacttggta ttgtccaccg ctatccacca cactaattac accaagtgtt
     5041 ttttctaagg ctttcgtatc ggctttcctt ttatctttga gttcaaaacg cagtctggtg
     5101 gcacaatggg ttaagctgac cacattttct tttcccccaa cctgttgcaa tacatttctt
     5161 cctacttctc tgtaatccat tattctctct ccttaaagca gtaaaaaaga ccaagtcaaa
     5221 ccacgcccca atcaacattg aagtgttagt ttgtcttggt cttgcctgca taatcagtaa
     5281 catgccaata acgttatttt gctgtaatcc aataatagca cattatgata gcgcaatcaa
     5341 cagggaattt aattttttat cttttcgttt gaccgtatag ggtaatataa cagttagatc
     5401 acatcaacta gggtatatct gcgtatactt actgctaaaa taagtatatc taatattaac
     5461 tataccagta aaaccattcc tacataacca gcgggaaatt cccaaaatcg tgaaagcatc
     5521 aaaaatgcat ataacggatc acacaacttc aacaattctg ctcttgggaa aaattgatga
     5581 atttggtgag taaaggatgg aataaacagc attccaacaa tataggcaac aatagtgata
     5641 cttgtttttt tggtaaaaat cagtaagcaa tagccaattt caatcattcc gagatataaa
     5701 ggtatcgata gaatgatttg ggctagtatt cttaaaaatt aactagcaaa tgtttaaact
     5761 gatttatttc taatctacat gtagtaagta tttcaattcc accaattatc aataaaaata
     5821 ccaaacatat ccataataat tttttctttt tcatttcttt tatattgtta taacagtgaa
     5881 taacataaaa attcccgaca atcattgtaa tatttttttg tcacctgtat tagtatttaa
     5941 tttataggaa actataaaaa ctccctagtg aatttacacc tttgggagtt cctgtttgga
     6001 cggtatgcat aatacattta aatcaattta acattaacac ctcagcatat ggtatctcca
     6061 catatatacg gtctttcttt ggatagtact caatatttat gttgatagca cttccattgt
     6121 ctaatatagc tttagcccgg tatgtgttat ctccattttc atgagtaatc gtcacatcat
     6181 cacatgatcg atttaagttg tactgttctt gtaaaatctg tgttaccaat ggttttgatg
     6241 acttctctaa tccagatgta cctgtcattt ttgaaaaaat aatacctata atagccaaga
     6301 caacaatacc tataatgaca aacttcttgt tcatatttac tttattcaac tttttatctc
     6361 ctatctccaa aataatttgc tcttatccct cttatcagta cattgacatc cctaaaagta
     6421 caccaaagat tgctccagct acccctgcta tcatcaaaat cgtacccatt ttctcatctt
     6481 tttctcgata taagtagccc ataataacag caacagcacc aaatacaaca ggaataaatg
     6541 ctaacgatac taccatagaa atccaaccaa taatcaaata cagcttccct gtctcttgtc
     6601 catgtacgat ttgttccttc atttcattaa ttgtctgctc agtacttttc tttactgtgt
     6661 ctgttccggt tgtctcatct gtttcttgta gtgctccaca aaactggcaa tacttacttt
     6721 catctgttat ttctttgcca cattttctac aatacatgtc ttctcctcct aaaatcattc
     6781 aattgcttat tagttaactg tttactttat taaatacatc agaaaacagc tcattcattg
     6841 gtacacttaa tatctcagaa agcatcttca tttgataggg tttaggaatc gttctccctg
     6901 tttgccacga acttacacat tgctgtgtaa cattcatttt ttttgctaac tccgtttgag
     6961 aaagtccttt ttcttttctg tatcggctga gattacttac ttttttcatg attctcccct
     7021 cctgttttat tatatacaac aatatgatgt atttcaactc ataaaatata aaaaacttca
     7081 aataccatta caaatacaac aacttgttgt actatactga gatgggggtg tattaatgaa
     7141 acagaaacaa tatgcaaagg tcattagaga aaagcgaaaa gctctcgggt ggacacaaaa
     7201 agaattagct gaaaaaatct tctctacaca acaggcagta gcaagatggg aaaattctgt
     7261 aacagagcct aacctcgata gcttaacagc tctttctaga gccctaggaa cccctgtaag
     7321 ccattttcta gacaatgttg tcgtagatta tgaggaagag tttttggctc tctatcgttc
     7381 tcttagcact gaagatgcag ttcgaacaat tgactatatg aagctattaa aaaggcaaga
     7441 aaatgaacgc aatcagctat tgaaagattg aaaaataaat aacccaccga actttaacgg
     7501 tgggtgtatt gagatgaaat aagattaaga gctatggata acctgtctag tttaatacaa
     7561 ttgggtcttt tctcttcctt actctccgtc catcatctct tgttacactt gtaaattgat
     7621 acagaggaac tatcccagct cctgataata tcaattcttt tattgtttca tctatccctg
     7681 cccctttcgc taaatatatt aaaaaactac tacttgcttt cacattgtat agtttcttaa
     7741 tatccttaga agctcgttca agagtgaatc cttgtttaac aaagtagcct ctgtttttta
     7801 ttaaagcatt tttcacgctg ttatccccaa cattacgtag gcgttcacaa acagtgttaa
     7861 taagttcttt ctctgtcatt agatatgtcc cttgccacga taagccctgt aaaacttcat
     7921 gtgcttcatc taaaatcaat tgtttttcct tgctgagttc tgatcgggaa ttttgaggaa
     7981 atactctttt agcctctttt tcgttaaaac atatcttaac ccattcataa gaaaaatatt
     8041 gatatgtaaa atgaattttt tccagaagtg gaaggatatc ttcattcaat ttttgaatca
     8101 cagaagttgc attaaaaacg tctaatcgat agacattgct tctcttatga ttaaagtttc
     8161 ctaacgcttt ctcttccttt aacctattta ggtattcgag catcttcaaa ggaacatctt
     8221 catcatctaa tttttttatc aatcctataa aagtcataag tgatagtaac ttattacatt
     8281 tatctatgtc ataagtttta ccatagtaag aactcatttt tttagctaat tgttcccccg
     8341 acaaaataca agtcacactt tccttactat catcaaaaaa attaacatca gaaagcaaga
     8401 catcatagac acgtcgcatt tcctctagat aattccttat tgtcctgaaa agtttgtctt
     8461 tcattgattc aagagtgtca aaaagtactt ttgattcctc tttcaatttt cgaaaatgtg
     8521 taggatacaa gtctaggttc tctaatagcc aattaaatgt ttctacccga ctgtctttct
     8581 ttaaaattag catcacaact ccaataaaat tttcactgat atcacaatta ctggaatgac
     8641 aataaaaaat ggaatgccca gttttactgg taaaaatact agcagaggga ttatgatcgt
     8701 tgtgaaacag acatcttaag tttttacttg acttaagtaa tttattcata tcaatggtta
     8761 acaatctttc ataaatctca ttaaaagaca tgtcggagga atcaaacagg cagtctttaa
     8821 accactgttt catttcttct ttgtcgtcat tttttatcaa cttaacaaaa tcggtttcat
     8881 tgactagttc atactctctc cctgaatcac tttgacaatt tttagaacat gtcatggtaa
     8941 aatcaatcgg aagctcagta atatctaaca tgttgtcttc attaattatt atcactgcat
     9001 ctgacttata tcctccaaaa aacattctat tcgaagcatt tacattgtca tcacacccgg
     9061 ggatgttctt tttcaagtat tcgtaaacag caaccatttt accatagtca gtaattgctt
     9121 cttttaatag aaagacaatc ttgtattttt ctagattttc agagtaccga atactttttt
     9181 gtatcgcaaa cgcagaatta tgaactaaat tgatagataa aaccttttct acgcttaagt
     9241 atctactatc atctttactc aaatctacta ccttcccctt ctcatcttta acagtgttat
     9301 cgacatccag aataattact ctggtttcca caaaactctc cttgtcattg cctttattct
     9361 ttgtcttgct tggaataaat gattgtgatg aatttagtat ctctacaaac tcatctcttg
     9421 taacttctct atagttatct aaacacctat ctcttacatc agttttttct ttttttgtaa
     9481 ctggcttctc taataatttt tcttgatcta cataaattgg aatattaatc attgatgttt
     9541 ccatttgtgt ttcctcctta taaaattctg ttaaagacta aaacatacag ataataaaaa
     9601 caaactcttt attactatat gttttagtct tatttgttta ctcaaccttt ataccgttcc
     9661 atgaaactat caatatcaga ttttttaaat ctaatgaccg tatcaatttt tataacaggt
     9721 aacccgtatt gagaaacata ttttctaatc gttcctactg atacacctag atattcactt
     9781 gcctgctgat agtccatata ctctagagtg actccagaaa taatgtcttc tagcataggc
     9841 tttagcactg taattatctc tttcacttgt tgttcaactt gtatagaaac aatgctatca
     9901 atgatttgta gagacatatt tccccctcct ttcgtgattg cttgaacaaa ttttctaaga
     9961 attatcttcg tatttt
//
LOCUS       DACDIH010000088         9919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDIH010000088 DACDIH010000000
VERSION     DACDIH010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.088"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(84..290)
                     /locus_tag="IV281_002397"
     CDS             complement(84..290)
                     /locus_tag="IV281_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781368.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(306..920)
                     /locus_tag="IV281_002398"
     CDS             complement(306..920)
                     /locus_tag="IV281_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP8781369.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(1067..1750)
                     /locus_tag="IV281_002399"
     CDS             complement(1067..1750)
                     /locus_tag="IV281_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8781370.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1859..2317)
                     /locus_tag="IV281_002400"
     CDS             complement(1859..2317)
                     /locus_tag="IV281_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP8781371.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2401..4164)
                     /locus_tag="IV281_002401"
     CDS             complement(2401..4164)
                     /locus_tag="IV281_002401"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP8781372.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4385..5845)
                     /locus_tag="IV281_002402"
     CDS             complement(4385..5845)
                     /locus_tag="IV281_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781373.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(6053..6979)
                     /gene="glsA"
                     /locus_tag="IV281_002403"
     CDS             complement(6053..6979)
                     /gene="glsA"
                     /locus_tag="IV281_002403"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP8781374.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(7002..8429)
                     /locus_tag="IV281_002404"
     CDS             complement(7002..8429)
                     /locus_tag="IV281_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8781375.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8460..9674)
                     /locus_tag="IV281_002405"
     CDS             complement(8460..9674)
                     /locus_tag="IV281_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP8781376.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2937 a         2050 c         1640 g         3292 t
ORIGIN      
        1 ctttcagttg caccaggatt ctttgataat tttttagttt tatgtgtttt tttatatgtt
       61 ttttcacttt tcggaataaa tatttatttg ttaaagtaat taaacaatcc gctaatcgtt
      121 tgatctggca tttctatttc cttaccatct aaagtaacag tagtaacgac aaaatggtct
      181 tgcaatggcg gaacttctaa gtactgccct tgcagaaaat cattcactgt ttcatattct
      241 tttttcattt cttttgaatc tgtcgatggt ttatagctta tttttaacat aactagtgct
      301 cctttttatt cgggacgtgc aatcgtgaaa atttctccta ttgctgcata atcatttcct
      361 gccaaacggc ttacagcatt caattttctt ggatcgatcc ttcctgtctt atcatcatag
      421 actgtttcat ttaaatgata tttctgtact tctagcaaaa ataaatcagc tgttacttta
      481 tttttattcc gtatctcgac atgttcatat aaggatgttt caaaacgaac ttttgcttct
      541 gtcaatgctg gtactgaaat gattactgaa tcagttaaag tcaagtcagt caggtacagt
      601 tcgctttcct cattaggcag attggctgct gttttattta cttcttctac gttgtcctca
      661 tcaaggatat ggataacagc ttctcttgtc tcaagaatat tctttgctgt gtcttttact
      721 gcatcttgtt ttctctggat cgccaaggat aagatgggtc gattcgaact gacgatattg
      781 aagtaactga agggtgcgat attcactata tcttttgctg atttagtcgt tactactgct
      841 actggtctag ggatgactga accaatcagc aacttgtaat tttcacgttc tgtcaattct
      901 gaaggactcc gttcaaacat ggtttcgcct tctttctata aaattcgtta aaaagcagat
      961 cttgaagaca aaatgaaaag taatgctttt ttcatcttct tagaatctgc tttaaactat
     1021 ctatcaattt ttcttttttg tcaagaatct gtgccgtgcg tgttttttag ttatttgctt
     1081 tacttgcggc ttcgacatat cttttagtga tcaattgcgg acgggtgata atagatccaa
     1141 ctacgactga aaaagcacct aattcatatg ctttctctac ttgttcagga gagtcagtat
     1201 gtccttccat aattactggg atatcgactt gttctatgat ttgattcaat aaatcaaaat
     1261 caggtgtctg cgtctttcgt gaatattctg tatagcctgc taaagtcgta gacaaaagat
     1321 caaaaccatc ttcttgcgct tgaagcgctt cctcaaaagt cgaaatatct gccatagcta
     1381 accgaccgtg ttcgtgaatc atttggacaa gttcagcagt tgttttttct gtcggacgtt
     1441 tacgagaagt tgcatccagt gcaatgattt ctgcttgcgt ctccaaaagt tcttccactt
     1501 ctttttctgt cgcagtaata aacacttctg acccatcgta atctcttttg ataatcccta
     1561 ttaccggtaa atcaacgatt cgcttgattt cattgatatc ttctgtcgta tttgctcgaa
     1621 taccaactgc tccgccttct tttgcagcca aagccattct ccccataata aaagaactgt
     1681 gtagcggctc gttttccaaa gcttgacaag aaacgacgac tccgcctttg atttgttcca
     1741 gtgtatccac aatatctcct ccagtaatgt tatataaaaa tacagcttca tataaaaatg
     1801 taaccctttt ctattcagga gacaataatg agctatttct ttcagcagac ctttcttact
     1861 aaaagaaaat tacttcaaat tcttcgtata cgaccagctt ctcttctgta aattctatgt
     1921 tcaacgcttc tttataagca tgtttaaaaa actcagcatg gactttccgc caataagcta
     1981 atgtccgatc cccttctcct tctttccaag catgctcttt cgtgacttca ctaaaaggca
     2041 agatggtcac atttatattt ttgatgatac aaactggttc attttttcca ttcaggacca
     2101 cgtctaattg accaactttt ggtaaaggct cattctcttg tttatacaga tcgtaaccac
     2161 ttgatgtacc agtctttatc ttttttacaa ctagttcgct caactcgtct gccatttttt
     2221 ctgtatcccc aaatgaaaag aaacctaatt tttctgattg ataactagga tatttttctt
     2281 ggaacttttt gacatattct tctacttgct gatacataac atccacccct ttgcttttta
     2341 taatgaaaaa accatccaga tactagtata aacaattatc tggatggtga gcataatgaa
     2401 ttattgaata ttcaagacac gcagactatc ttcaacgatt tgttttggtg ttaaatgata
     2461 acgttcataa agctgcccta caggaacgct atccgtgaat tctttctctg caccaaaatt
     2521 taatacgttc attgaagaat caccataata acgactgata ttttcgccaa atcctcctga
     2581 caaactacca tcttctaatg tgatgactaa ttcgtgtgtt tcttttaatt tatctaatgt
     2641 ttctacgtca ttttctgtga tcgttcctgg attgatcaac gttgcttcga ttcctttttt
     2701 tctaaaagct tttactgttt cttttcctaa ttcaagaaaa ccgcctaaag ctaaaattgc
     2761 tacttttcct ccattattta ctgtttgata ggctggaata ctataatcat ttgcgatagc
     2821 tgtaccatgt tctacaccat gtgtaggaat tcggattgct acaggctgcg ctgtttgttc
     2881 gaacgcccaa tctagcatag agatcatatc ctcttttgtt gctggagcaa gatatttgat
     2941 gttcgggata cttgataagt aaggaatgtc aaatgttcct tggtgtgttc ggtcactagc
     3001 tgagatcgtt ccgccaccga ctaaaataat cactggattc tcgttgatcg ctaaatcatg
     3061 tgataattga tcatatgccc gttgcaagaa agtacttgat tggaagacga ctggacgtac
     3121 tccctgagaa gccaaagcag aagcgaaagt gatagagtgc tgttcagcga tccccacgtc
     3181 aaaataatga tctggatgtt tttcttggaa tgttttcagt ccaaaggcgc ctggtatcgc
     3241 tgcattgact gcggcaatag gagctgtact tgtttgcatt ttcttatcca aataatccat
     3301 gatgatcata ttaaatgttt ctccagaagt tacttgcttt gcctccccgg tggacagatc
     3361 aaatggtaca tgccaatgat atttcatttt attatcgatt gccggctgat aaccgcgacc
     3421 tttttccgta tggacgtgaa gtacaatcgg atgatcaata tcttttactt cttcaaataa
     3481 acggatcacc gtttccagat cgtttccatt atccacgtaa cgataatcaa atcccattgc
     3541 tttaaatagg ttattgctgc ttttaccgtt ggtttgacga agctcagcta agtttttata
     3601 taaactgcca tgattctcat caatagacat ctcattatcg tttactaata cgattagatt
     3661 ggattgtaaa acaccaccat tgttcaatcc ttcaaaagct aagccaccac ttagggagcc
     3721 gtcaccgata aatgctacca catttcctga ttctttcttc agatctctcg ctttagctaa
     3781 acctgttgct aagctgatag atgtagatgt gtgccccact gtaaaatagt catgtttact
     3841 ttcatgttgg ttggaatatc cgctcacttc acggtaatgt cctttttcaa aaccattttt
     3901 tcgaccagtt aaaagcttat gaccataaga ctgatgggaa acatcccaaa cgattttatc
     3961 ctttggtgaa tcaaatacat aatgaaacgc aatcgttaat tctactacac ctagattcgg
     4021 accaacgtgc ccacctacat cgctgtcctt ttgcaagatt ttttggcgca tctcttctgc
     4081 caactgtttc atttcctcaa cagataattt tttgacatct gcggggctat tgattgcatc
     4141 caatacatgt aatactgtac tcataataca ttctctcctc cgtactatct tcttttaatg
     4201 aaaatagatt tgttacactt tatctattct actacttaga gttgactcta agtcaatagc
     4261 attttaaaaa aatttcagag aaagcttgtt atataagtag atcttcgcac gaatcaggac
     4321 tcaaataaaa accttctaca gacaattaaa aagctttgta gaaggtctcc taattaaggt
     4381 ctcatcaaga aatatgtttt tttaataggt gaagttcctg agcaatatga tacaaatgga
     4441 tgtttgcacc ttttaatgag attggataga tctttgtctt ttcagtttct aatccatttt
     4501 gatagctagc agcatcgcac aaaaccactc tttttttcgc acatagacat tcatcaggca
     4561 attcttctaa tgaacgaaat aaaataggat ttatttcagc gatgccttct tccgcataca
     4621 aatgaaattg ggttcgataa aaaaattgtt tgactgaact ttctgaaaca acaaaaattt
     4681 ctatttcctg tctccccgct tgttcactgt tgatcaaaga aagacttttg acgaattttc
     4741 gaatgaaatt ttcattccat cttagttgga tttgattttt ctttttccac tcttccaata
     4801 tttctacaat ttttccgtac agaagttgtt ctttttctga tagaagatag atcctttccg
     4861 gtaaaaatac ttgtccatct gcccacaacg tacggacaaa ttgtaaaaag gattgcttga
     4921 aaataaaatt ttctgcatcg tcgatttcag cgatactaat aatctgtaaa atcaattcat
     4981 tcagtgatag gtcattgaga acaaaatttt tataaacaac ttcgaaatct tttcttaaca
     5041 gctccatgtt ctcattggta taatttctac tgttgaacaa agaaaaaata taatagacat
     5101 cttcttcttg taagtctaga attccttttt gctcttccaa tccttgacag actaattgaa
     5161 ataacggctt cgtttctgcg cttttcttat cttcttcttt cagtccaagg ggaaatgcct
     5221 ttcttccgat tgcgatctct attcctcgta tgataaagga cttttcatct tccgaatatc
     5281 ttcttttaaa aaaatgagtc tctacataat caatcaactc gttacaagct tcttctacat
     5341 acggattttt attttcatag ccttcccaat tgacataacg tactaaagct agcaagatgt
     5401 tccgcgagtc aaattctggg ataatgattt ctttattttt caaataatca ttttctttaa
     5461 aaaaatccaa tacgattttt ttgattgtat atactttgct caaagaaata aattcaagat
     5521 cagccagttc tgtactcgtg aatataccat taaaataata acttaaaaac tttaaaaaat
     5581 aagattgacc gtacagaaac tgtgtgagct cagaaagagc aataccatgt ttgaatacga
     5641 gtttatactt atgcttctct cttacttgtt cttttataca atattctaga cactcggcta
     5701 gactttctag atcgtgaagg atcgttggtg tagtcaccct tagagaaata gctgtttgtt
     5761 cgatgtctat ttcttggaat tcaaataatt gttcacacag atacactttt ctaaatatat
     5821 ttcgctcgat gtaattttcc agcattcctg atacacgcct ccagtttttc ttatgatttt
     5881 atttaatcat atctgtaaat cgtatttttg tttccttttt atttagaaaa gccctttcaa
     5941 aaaccaataa aaaaagaaac tgggacaaaa gtcaaacaag ctcacaattt cagcctcatt
     6001 gtaggtagct ttgagccgtt tggactttta ccccagcctc tttcaagaat ggttattcaa
     6061 agatgtcagc gtctagttct ttgactacat ctttcaacaa ttggatacca gctgcgctgt
     6121 taccaaatgg atctagtgcc gggctgaaga cacctatgcc gtaacgattt ggcacagctg
     6181 ccatcaaccc gccgcctaca ccgctttttg caggtacacc aacatggacg gaaaactctc
     6241 ctgattcatc atacaaacca gcagttgtca taattgattt gacgattgta gcactttctt
     6301 cagtaatgat ttgtttgcca ttccaaggag caattccttt atttgccagc acagcagcta
     6361 attttgctat gcctgtagcg gtgacattga gggagcattg tctgaaataa gcatcgagta
     6421 tctccggtac gtctccttcg atcatttgat tacctttcat atagtaagca agagagcgat
     6481 tgatatcacc tgttcttgat tcagaatgat agatttcttc attcaatgtg atattaggat
     6541 catcacagat ctctttcata aatgacaaga ttcgctcaaa tttttcatct gcatctttac
     6601 cggcaatcaa cgaggttgtt gcaatcgctc ctgcattaac aaaaggattc attggatgtt
     6661 tgcgatgatt gatttccata ttcatgatag aattgaaggc aaaaccggtt ggttccgtgt
     6721 tgattgtttg aaagactttt tctattccgt tgtctttgat tgctaataac aatacgggaa
     6781 cttttgacat gctttcgatg gcaaagcgca catcggcgtc tcctccctca atcagttgtt
     6841 ttgtatccaa atcataaatc gccataccta attgtttggg atccacttca gctaatgctg
     6901 gaatgtaatc cgccacttgt cctgtttgat aatagtggaa attttttcca atcaattgtt
     6961 ctaattcttt tacttccatt tgaataacca gctttcattt tttatttttc tgcatgctgg
     7021 aatttatgac ggcatgcgta gatcgtaaat ggaataacta agacgataag ataagaaaca
     7081 atcagaatcg tttcataaat cccatgctgt ccagctgcga tactagcagg tggaacaaat
     7141 gagatacaaa aagcaaaaag tgaagtcaaa agacctaaac aagcaacgat tacttttaca
     7201 acttttccgc ctggaacctg ataggctctt tcaagactgt catctttaaa aaccagcttc
     7261 aaatagcccg cgaaaaacat aaagtacaac atcaggtaga ttacaacagt caatgacatc
     7321 gctgcaagaa atgaaagatt attccccccg ccgcttaatg taaagacggc tgcccagatt
     7381 gttgcaacga tcccatttgc gatcatcaaa tgagcaggaa caccgtgtgt gttttctttt
     7441 gcaaaatatt caggaagtaa tccttcttct gctgctacgt gaagtgcttt tgaaggagag
     7501 gttacccaag aagcaatttc tccgatgaca cctaaacaaa caagaatcgc aagaattttc
     7561 gcaatccatg caagatttgg atcaatgtgt cttaatagga attctaacga ttgaatcacc
     7621 cctgtattaa gagacaaacc atgttgaggt actgtagtag cgacggtcaa tccgccaaaa
     7681 gtatctaaaa tgattgccag aataaccaat acaaacattg ctaatggata atccttttta
     7741 gggttttcca tttcatttgc atgactggca gatgtttcta cgcccatata tgctaaaata
     7801 aaggaaacaa aaacaaccag cgtaggcaat ttcgaaaaat caggaatcaa tgttttcata
     7861 gacagtatcg tttgtagtgg agcgcctgaa gcgacataat ggatccctaa gccaaacaag
     7921 actaatgctg gcaacaagat accagctaca aaaccgatcc gagcaatcaa tgaagtgtat
     7981 ttggttcctt ttaactgtga gagtaaaata gcccagaaaa taatcaatag gattgccaat
     8041 ttcagatatt tattgttttg tatagcacca atacccgttg cataggataa tgcaccagtt
     8101 aagaaataca gcatggtcaa gtagccaact gttatctcaa accattggaa aaagattgcg
     8161 gcaaaaccga atcgttttcc caatgtgcga ctgacccaag tatagacacc gcctttttcc
     8221 catcctttca ccgttgccat ctctgctgcg cacaatgcca cagggataaa ccatagcaaa
     8281 ccgcctaata acaagaaaaa tactaagctg aagcctgagg tcgcgaatgt gggatattca
     8341 tataatgaaa cgaccattga gatggtcata gccaaaaagc caaagaacgt taattttttt
     8401 gcaggaattt tcgttttgct ttgttgcatg aatcttcctc ttttctattt gaatttgttt
     8461 caattgagtt tgttgccaaa gaagaaaaag tccaatcctt cttcttgttc ttcttttgta
     8521 gtagtgatcg ttgtaaagtg catcgccgat tttaaaatca ctgttgtcag cacataggca
     8581 tacacggcag taatgatgac accaatcact tgaatcccta agaagtacca gctgcttgga
     8641 ttcgttagta aatggctgtt agatgcaaag atccctatca gaatcgtacc tgtgaatccg
     8701 cccatgccat gaacacccca cacatcaagt gcatcatccc attgtttctt tttacgaaaa
     8761 tcaacagcag catgacagac gatacccgca atgatcccaa tcagcatggc actttttgct
     8821 tctacataac cggcacaagg cgtaatggct gccaatccag caacggatcc tgttagtacg
     8881 tcgacaaaat caaagccatt tccttttact tttgcaatga tcagccaagt aatcatcgca
     8941 aatgcaagag cgatgaatgt tgaaccaaat gcattgactg cttgttcatc tgctcgaagt
     9001 gcaccaccgg aattaaaacc gaaccagcca aaccaaagca gtcctgtacc gatagcagct
     9061 gccataagac tgctatgctg acttctttct cctaaaggaa tctttctttg accaagtgtg
     9121 aggatacatg ctaaagagcc gaatccagct gtcgtgtgga tgactgctcc accagcgaaa
     9181 tctctaaatc ccatctgatc caaaaatccg ccaccccata tccagtggca aacagggaaa
     9241 taaatcagca gattccaaaa aataacaagt ataatatagc cttttaaatt caatctccct
     9301 gcaaaagctc ctgtcatcaa aggaacggta atcacgataa acatcagttg gtaaagaaag
     9361 aacattaaaa atggaatcgt tgcaccatga aacatgttgg ggaagcttgt aacatgtctc
     9421 atcaaaaaga aatctgccgg gtttccaata acaccgccga tatcccgtcc aaaggccaat
     9481 ccgaatccac caaataccca cataatcgtt gttatcccaa cggagagaaa agattgagac
     9541 atgatcgtta gtatattatt cttccgtgct aatccgccgt aaaaaaaagc tagaccagga
     9601 gtcatcaggc aaaccattgc tgtacataaa atcatgaatg ctgtatctcc tgtattgata
     9661 ggtaatgtat tcattttttt cgcctccttt ctacgattac gagtataaaa gaaggtgtag
     9721 ttgttttttt cttctgtttt tttgaattaa tacttgcatt tataaaagac agacaattaa
     9781 taaaagcttg atgtgttgtg ctctttttcg tttttcacac aaataaagac aaaaaataaa
     9841 cggtcttctt tttatagaaa accgtttatt ttcgtctttt tttattttta aagatatttt
     9901 ttcataagga ataaagaat
//
LOCUS       DACDIH010000089         9853 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDIH010000089 DACDIH010000000
VERSION     DACDIH010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.089"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            76..1527
                     /locus_tag="IV281_002406"
     CDS             76..1527
                     /locus_tag="IV281_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8781377.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1876..2547
                     /locus_tag="IV281_002407"
     CDS             1876..2547
                     /locus_tag="IV281_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP8781378.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            3095..4534
                     /locus_tag="IV281_002408"
                     /pseudo
     CDS             3095..4534
                     /locus_tag="IV281_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4879..5046
                     /locus_tag="IV281_002409"
     CDS             4879..5046
                     /locus_tag="IV281_002409"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8781379.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
     gene            5305..5520
                     /locus_tag="IV281_002410"
     CDS             5305..5520
                     /locus_tag="IV281_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781380.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5708..5995)
                     /locus_tag="IV281_002411"
                     /pseudo
     CDS             complement(5708..5995)
                     /locus_tag="IV281_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(6182..7093)
                     /locus_tag="IV281_002412"
     CDS             complement(6182..7093)
                     /locus_tag="IV281_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8781381.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            complement(7112..8116)
                     /locus_tag="IV281_002413"
     CDS             complement(7112..8116)
                     /locus_tag="IV281_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP8781382.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            complement(8113..9738)
                     /locus_tag="IV281_002414"
                     /pseudo
     CDS             complement(8113..9738)
                     /locus_tag="IV281_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
BASE COUNT         3047 a         1772 c         1873 g         3161 t
ORIGIN      
        1 ctcaattcat tgaaaatatt tatcataatt tttaatagaa aacactatta aagagaaaaa
       61 agagaggtaa atcaaatgaa aaaatcttta aaacttgcat cagcagttat attaatgtcc
      121 caactcttta tagcaacacc tttccatgtg ttggcaagcg aaaattcaac atacaaaatt
      181 gcaaaagaaa gaaccgagac gactgaaatg agtaattccg atgtaacagc agagagtagt
      241 gaacttttga caactttatc aagtgaaagt agtacgactt caaatgctga gtcttcaaca
      301 acttcatcaa gtgaaagtag tacgacttca aatgccgagt cttcaacaac ttcgtcaagt
      361 gaaagtagta cgacttcaaa tgccgagtct tcaacaactt cgtcaagtga aagtagtacg
      421 atttcaagtg ccgaatcttc aacaacttca agtagtacaa gtgaaataac ttcttcgaaa
      481 gtgacaagac caaaccatat agagtcttca aaaaaggaag cgccaaaaac ggaaggacaa
      541 acaaaaaatt tttcgacacc acagcaaata gaatcaacag aatctttcca atcacaacca
      601 atactatctg gtaatgaaga aattcatttt gacaaaaatg aatctgtaga aaattttatt
      661 atgaaaattg gtgagtctgc gagaaaaatt ggtcagaaat atgatttata tgcttctgtt
      721 atgattgcac aagcgatctt agaatctgcc agtggtcaaa gccagttagc tcaggcaccg
      781 aattacaatt tatttgggat aaaaggaact tataatggaa actttgtcat tatggtaaca
      841 aacgaagatt tgggtaatgg aacgctttat acgacccaat caaaatttag agtgtatgaa
      901 aactatgagg aaagttttga agattatgcc aaattattaa ctaagggaat tagtggaaat
      961 aaagattttt atgcaggagc attaaaagct aatagtaaaa catatcgaga agcaactaaa
     1021 ttccttacag gacggtatgc aacagataca caatactatt tgaagctaaa tgaattaatt
     1081 aaaacctatg atttaaccaa ctatgataaa gaagttaaag gtccacaaat gagtaagact
     1141 ggataccaag tgcctattca taattattca ataaccagtt tgtttggaaa tcgtggaaat
     1201 gaatttcacc gaggacttga tatggcagct acacagggtg agccgattca tgcagttaaa
     1261 gctggtaagg ttgttaaagc agagtttcac tactcttggg gaaattacgt ggtgatacaa
     1321 cacgaagatg gatcaaccgc gttatatgct caccagcaac aatacctagt aaaaactgga
     1381 gataatgttt tgcaaggaca aataattggc tatgtaggaa gtactgggaa tagtactggc
     1441 ccacaccttc atttggaaat ttgcaaagat agtagtcttt ctcagagcat gttagttgat
     1501 ccgaaaacct tattattcgg agactaaaaa aggtaatcaa tctgacaaag taacaagaaa
     1561 atggttggat tcttagacct ttgatcagga tattgagact ggaattgata tctcgattac
     1621 gatgagtttg acaaataaaa caagtccatt atcgaatatc gagtgtatta ttgccgtctt
     1681 tatgctcata ttttgagcag gttttactag atgaaaacaa tttatctatc ttgaggattt
     1741 cgtgtacgta tcgttcagtc ttgtattata aattagtcat aaaatcagac taagaaaaat
     1801 tgaaaatatt tttttctgat ttattaaaag ttaattgaaa ttatatggta cttctttctt
     1861 gggagggtag gtgcagtgaa ggaaagatat ggtcacctta ctgttgttga ggagattact
     1921 cataatggat ataaaaaata tcgttaccta tgtgactgtg ggaatgaaac gattgtatat
     1981 cgatcaaact tactatcagg tagaaccaaa agttgtggat gtggaaatta taaaaatagg
     2041 cataaatata aaaatttgac aaataaaact tttggtaagt tagttgcaat caagccaact
     2101 aaactgcgaa gtgaaggaac tgttgtttgg gaatgtcaat gtctgtgtgg gagaaaaaag
     2161 ttagtatcgg caaagcgact tctaaaaggt gatgtaaaaa gctgtggatg tttgaaatca
     2221 aaaaagcatc acattgaaaa taaaaaattc ggattactta ctgcaaagta tcctttggac
     2281 aataactgtt catggaaaac aaaatgggtt tgtcaatgtg agtgtggcag tatgtgtgag
     2341 ataagttata gtaacttatt ttttggacat actcaaagtt gtggatgttt aaaaaggcaa
     2401 gaaacaagga catgtgttga agggacagta atagagtgtc taacgtcaaa attaccgaaa
     2461 aataatacaa gtggtgttaa gggagtttat cgttctaaag agaaatggat tgcgtatata
     2521 actttaaaaa aaagaggtac tatttaggat cgtatgattc tattaagagt gcagctcaag
     2581 ttagaaaaga tgctgaaaat aaattgtttc aacctttgat tgacaaatat cgaggtaatc
     2641 attaattatt gtggataata attaacagtg atatataaac atctataata tattattcat
     2701 actattgaca tttagtataa tcctatggaa ttgaatatca tagcaatgta tttgaaaagc
     2761 aaattggaaa acaaataggt gcaacaccat aaatatgaaa aaattgggaa aaagtagaat
     2821 ggaaattttc ttacgtctca cgagatagct cacactatta atctgaagaa caatgaagtc
     2881 gacttttaga aattgctaga agaaagtaaa gcgaattatc actagttact atagagtaag
     2941 ttcaaataaa gcatctcaaa cttacatggt tacatgagaa taacaaatcg aaattactca
     3001 agcttcctat ggctagagtc gcgagaagtt gttgggcagt agacataggt tatattgttc
     3061 ttagctacac gatcattaga aaggaaatcc tattatgcat agttatcaag aagatgcaaa
     3121 agtccaaaaa aatcgctggt ggattttgat tgctatatct atgtttacct tcatgtccac
     3181 attggatggc agtattgtca atattgcact acctaccatc tcagatgata tgggcatacc
     3241 aatgaatcaa gcggaatggg tagtgtccgt ttatctgatg attgtctgtt cttgtttgtt
     3301 gctgtttggt aaaattggcg atagctgtgg gaaaatcaaa gtatttcgga ttggtacgct
     3361 gatctttgtc ttgggctcgc tgttgtgtgg gttcaatcat tcgctaggta tttaattgtt
     3421 cgcacgggtg gtacaagcca ttggtgctag tatgactatg gctacaaatg ccggaatcat
     3481 cacggaagtc tttcccatac atgaacgtgg tcgggcattg ggagcaatcg gtgcgcttgt
     3541 ttcattaggc tcgattgctg gaccaggtct tggcggcttg attttgtccc aattttcttg
     3601 ggcgtacatt ttttggatca atgtaccggt gggaatcgta acgatgttga ttgctgaaaa
     3661 attcttacca aaagatatca ccatgagcgg ccatacaatc gatggagggg gctttgctgc
     3721 cattgcggct acgattatca gcctttttgg aacaatcttt ttggggcaag aaagtggctt
     3781 tttccatcca gtcccccttg tactgttcgt aatttcagtg attgcctttg tcagcttttt
     3841 aaaagttgaa aagaaagcag cctctccatt gatttctttc ggtattttta agaacaagat
     3901 ttttaccatg agcttgatta cagccttgtt gattttttct tccaacttct ttgttaatgt
     3961 agtgattcct ttttatctgc aaaatgctcg cgggttgagt cctagctatg ccggactgtt
     4021 gatgatggtc tttcccttga tgatggtcgt cggggcgcct ttaagcggat acctgaccga
     4081 ccgggttggc ccggagattt ttgtattagt aggcttgggt ttattgtcgg taacccaagt
     4141 catgtatatg tttttgtcag tggactctcc tttgtggctg tacattgtcg ctactgccat
     4201 catgggtgcc ggcaatgcct tgttccagtc cccaaataat acgatggtta tgagttctgt
     4261 cactatcgaa aatttgggac tggcaggctc catgaattcc tttgcccgaa atttggggat
     4321 ggtcatcggg atttcgcttt ctacgacggt cttatatcaa gcgatgtctg ctaagatggg
     4381 ccaacgagta actaactata tcgcagatcg gccggatgtc tttttatatg gaatgaagat
     4441 taccttttta ggcagctttg ttttgtgttt tgttgccttt ttcctgacta tctggcgtat
     4501 caaaaaacaa ggaggtattc agaaaaatgt ctgaaaaacc attgttgaag aatttttgga
     4561 ggcctgcgta tgggtgagtg ggatttccat ggatgatgga agatgaagtg aacttctgaa
     4621 aaccgaaaaa ctcaagtatt atcttcaaaa tactagtact caattagatg ctcgatttga
     4681 ttacaagaag actgcgaaag ccactggcaa ccgcaagcta ggtatccacc gatggactac
     4741 gttttagagg caactggtta tgaatacgga gctaacgctc tcatctgcat aagaatgcaa
     4801 cacccagatt atgtaaagat tttcgatact tctgtcaaaa aaatcatcaa gatagataaa
     4861 tgatttccgt ctagtcaaat tggttcagaa tgtggacaca cagatggtaa gaagtcattc
     4921 acaattcgag aatggacttg tcctatttgc cgtacacatc atgatcgaaa cgtcaacgtt
     4981 agtatcaata ttttgatcaa gggctatgta tacaagcatt gccttagaaa acatcaagaa
     5041 ccttaggaac aacggggata gattggttaa taagagatat cgctgttagt aaagacatag
     5101 gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgata ggtttaatac
     5161 tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata ataaaatgac
     5221 cactacagta acctactgaa atatataaac agccactgta gtaccatttt caaagtagtc
     5281 tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa tttaataaaa
     5341 aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat caatttaagc
     5401 acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct tttagatgct
     5461 ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat atggagataa
     5521 ttatcgtcta gtgaactact tgccacttag ctgaatttaa cgatccttag tgcttgaagt
     5581 gggagattct tgggaataga gcatacttgc aagcttatgt ctttattaac agcgatatct
     5641 cttattaacc aagctatccc cgtagttcta aggtttttac ttttatctaa gctaatgatc
     5701 gtattcaaag gaaatttttg acccttagaa agtctcattt tatcagacat gagcttaggc
     5761 attgctgaat tttgttaatt cggttttagg ttgtgctttg agcataatat gaaagtagtc
     5821 tttatcacga tttcactcaa ctaaggtaat gtgatgtgat tctgatattc tttcaaagat
     5881 agttttttgc ataagctaac attacatcat caatgagatg tttacaatgt ttcactacta
     5941 aaataaggtg gtaacaaaga agaaataatg aatgattatt tgtatctaat tccatggtat
     6001 atcaaccatt tcttatatag actgattatg acatggatta aaataaaaag gcactatgtc
     6061 ttaattgtcg gttttcgagt taccgaacgg cattcatcac ccacctatag aggatgggcg
     6121 acttctgcct gaaataagtt aaactactta taacttctca taaatttaga ttgagtcatt
     6181 attatttaac atcaatgatt ttccagtttc catttttttg taatattaat ttgtactgaa
     6241 agtagctagt ggtttttgat acattatcaa ggtattttat ctttgtactc actagaattt
     6301 tttttccgtt ttgtaggtaa actggatcct ctaacttcga aaactttaag tcaatattga
     6361 ttggtcgcat cgcatcattt tcaacataat actccaattc tttttcagaa gctgatggat
     6421 ataatttaaa gaacgtttcc aaaaactcag cgatttcttc tgtagttttg gcatcaattt
     6481 tcgaatcatt ttctacttgt tttgtcttgt attttgccct atcaggtttt gcaatcatag
     6541 ttggcaaact cgttactata taattcctgt tttcatcttc aaagatcgtc actcggtatg
     6601 cagaagaaat cgattgtgcc ttttttccct ctgtaatctt ttgctccact aaaaatgaaa
     6661 cgacaaattc ctccgagctc ttttcaacat ctaagacttt tactgactga acttcggaat
     6721 tagtgggaat atctgctcga accatatctt gacttaaagc gaaaccttct tctgtaaggt
     6781 actgttcgag gttgttcatt ctcttttcaa ttgcttcttt gttatttttc cacgaaaaat
     6841 attctttcga aaaatccttc gtaaagtttt caataccaga agtattcact actttctctt
     6901 taataaccac tttttcatga gttgtatgtt gatcaatggc ggtgaaattt ttataaacgc
     6961 caaaagtaag actacaagat aataaaagcc aaagggcaag caccattttt cgatgcttac
     7021 ccacggatac gactcgttct tttttctttt ttaggctctt ttccttttga ttccgttcaa
     7081 tttttatctt cattcttttt cctcttttct cttatttgta ccgtccagca ccgataagat
     7141 gctgctgcca atagctttct gttagattcg tccagcctac tggatctcct gcatgaaaca
     7201 tgcgattctc ccctgcataa ataccgacat gggtcacata agtaccggca tcataagtcg
     7261 aatgaaagaa aaccaaatcc cctggtttgg catcttttag gtcaatatgg gtcatggcat
     7321 catactgatc ttgagccacc cgaggaagtt tcagaccaac tttggcataa caccattgag
     7381 tcaagccact acaatcaaat cctgtttcgg gtcttgaccc accaaataca taagccgttc
     7441 cttcatactt aaacgcctcg tcaaaaatcc cttggactgt tttatcgtca aactgtgttg
     7501 tagttaggta ttgcttcacc aataagcaat aaaacatatt gccatagtta tagcgccatc
     7561 caccattttc cttattcgcg atgggattag ggtaatcgat tttttgaccg caagattttt
     7621 cctttgaaaa agattcagct aaagcaaaac tatgcttctt cccgtttttt gccacatagt
     7681 ccaaataacc accgccaaag ttataggcct gaatgatact atcttgatca caacctaatt
     7741 ctttggaacg cttaaccaaa gacgcaaaat agagacatcc ttgtttgatg gatgcctcgg
     7801 tacttaatga attcggtggt aaaccggctg actcagaact ttgcatcaca tcttccattg
     7861 tgccaccaga ttccacttga ataatcgcta aaagaatcgg aacatactct gttatatccg
     7921 attctttggc atatttttcc accattgcac ggtgtttcaa aacctcttct gagacactac
     7981 tccccccagg aggagcacct gaactactac ttccttcact gtcttcacca aacaataagc
     8041 ccacacacat caagataccc ataaaagaga ttgttaggaa agttaacgaa atggcactta
     8101 atttctttac gttcattttt tctcacctga ccttggtttg ggatgagggt tcaaactttt
     8161 ttgtactttt cgattggttc gtttggacag agtagaccgc cgtttcactt gttgaaaaga
     8221 atttttctta cttgaggatt ggtttattcc ttttgaaatg atttcttggc tagcgaattt
     8281 tcgtttgatt tcgggattcg aatttgctct cacaatccgc tttggtgctc tatccttttg
     8341 agattggacc tttgctttta tcggaacatt ttcccttctt ttcccatgag gattccctat
     8401 ttcgttcaat actttgcgct tttcagctat tttttcgtct ctacgttgtt gttgtttttc
     8461 tcgcaactct cctcgatttg ctttttcttg gaccagtcct cgcttaaact cttccggtgc
     8521 ttttttcgtt ttttcaattc ctttatggaa gttgtatttc aagttggttg gcgtattccg
     8581 tactccatcc tttacttgtt tggtcttatc tttcaccaaa gcttgggtat ccagaacctt
     8641 gccgactttt ctcccggcca aattcattcg ggattgtttc ttattatttg gaattggctt
     8701 ttccttattc aagtcagatg gtatctcgtg atcgttcggt aatcgtgaat tctttcgtag
     8761 agaagaaccg gatggcttaa atttggattt tgattgttcc tttgccaact tatttccaac
     8821 tgctgcacca ccaccaaaag ccaaagtccg tcccacattt cgattgagtt gccgcaatgt
     8881 tcgattcatt aacatttgcg gttttcgcat gacccgtctt cccattgatt ggctatcatt
     8941 actctggaga ttgaacatat tcataatgtc gcccaatttg aaatagatac cggcaaaggt
     9001 cacaatttgc agaaaagcaa ccatgaaaaa tggataggtt gccgaaatat tgaaaaacat
     9061 cgtggagatg ctaaatgcag tcgtaatcac caaagtaact cctgctcgca tcataatcgt
     9121 attgaacaag cgaataatcg ccttcttgcc caaactctca taagtcggca acatggacaa
     9181 taagaaacta atcggcaaaa acatcgcaaa gataataaag agaatttgcg agaacaacat
     9241 aatccctgtt aggagaaaga caaagaagga tatgccaata ttgaacaaca ccaaaaagac
     9301 cacggtccct aagcggttca tggtcttggt aatactgaga ttcgcattgt cattatcttc
     9361 aatctccgct ttgaccgctt cttcccgatc cttgccttta ttttcatctg gacttacaga
     9421 caggattttg ttaacacgat cttcgccaac ttcttctata ttggaatcat caaattgcaa
     9481 gagtaaccac ggttgttgga cttgaatgga aaacaagcta tctcggatta aatccacact
     9541 gtcctttcct tggctttctg aattcggcac gacaatctcg gttccaaggc taagagccgc
     9601 ttcgctgaca tctgagctaa aatcattgat ttttgtgata taggtgggcg catacgcaat
     9661 gaacgaaccc gaaagtagaa aaatcatcac aaaattaatg acagcccgga ctgctttggt
     9721 agtttctcgt tttaacagaa ttaggcagaa gtcgcccatc ctctataggt gggtgatgaa
     9781 tgccgttcgg tatgagggtt accgttggta actcgaagac cgacaactaa ggcgtattgc
     9841 ctttttattt tgt
//
LOCUS       DACDIH010000090         9683 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.090, whole genome shotgun sequence.
ACCESSION   DACDIH010000090 DACDIH010000000
VERSION     DACDIH010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.090"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            4..1458
                     /locus_tag="IV281_002415"
     CDS             4..1458
                     /locus_tag="IV281_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HAP8781383.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
     gene            1534..2694
                     /locus_tag="IV281_002416"
     CDS             1534..2694
                     /locus_tag="IV281_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAP8781384.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            2807..3814
                     /locus_tag="IV281_002417"
     CDS             2807..3814
                     /locus_tag="IV281_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8781385.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            3838..4864
                     /gene="iolG"
                     /locus_tag="IV281_002418"
                     /pseudo
     CDS             3838..4864
                     /gene="iolG"
                     /locus_tag="IV281_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
     gene            4881..5771
                     /gene="iolE"
                     /locus_tag="IV281_002419"
     CDS             4881..5771
                     /gene="iolE"
                     /locus_tag="IV281_002419"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HAP8781386.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            5794..7389
                     /locus_tag="IV281_002420"
     CDS             5794..7389
                     /locus_tag="IV281_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HAP8781387.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            7405..7719
                     /locus_tag="IV281_002421"
     CDS             7405..7719
                     /locus_tag="IV281_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8781388.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            8180..8905
                     /locus_tag="IV281_002422"
     CDS             8180..8905
                     /locus_tag="IV281_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8781389.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            9038..>9683
                     /locus_tag="IV281_002423"
     CDS             9038..>9683
                     /locus_tag="IV281_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8781390.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLY"
BASE COUNT         2956 a         1718 c         2234 g         2775 t
ORIGIN      
        1 taaatgacaa gatttttaag aaattatatt gatggtcgtt gggtcgctag tcgctcaaca
       61 gagtttgaag aagtaatgaa tccggctaca gaagaagcgc tggcgaatgt accgttgtct
      121 agcaaagcag agacagccga agcagttaaa gctgccgatg ctgcgtttca aacttggaaa
      181 aaagttcctg ttcctgttcg cggacgttat ctgttcaagc tgcatcagct tttaacagat
      241 cataaaagtg aattggcgcg tttgatcacg ctggaaaatg ggaaatcatt gtccgagtca
      301 ctgggagaag ttcaacgagg aatcgaaaat gtggagcacg cagcgggaat caccaattta
      361 atgatgggag attctttatc aacagcggcg actgatattg agatcacgaa ttatcgttac
      421 ccaatcggag taatcggagg gatcacaccg tttaattttc caatgatggt gcccttctgg
      481 atcttcccga tggctctggc ggcaggaaat acggtggtgc tgaagccttc agaaaaaaca
      541 ccattgttga tggagaaaat tgttgagctg gttgaaaaag caggctttcc tagcggtgtt
      601 tttaatgttg tctatggtgc ccacgatgtt gtcaacacat tgctgcaaga tccactggta
      661 aaaggcatca gctttgtagg atcaaaaaat gtcggtgaat acgtttaccg tgaaggaaca
      721 aaaaatctaa aacgggtaca agcgttgact ggagcgaaaa atcatgttgt tgtgctgaaa
      781 gatgccaata ttgatcgggc agtcaaggac cttattggcg gagcttttgg ttctgctggt
      841 gaacgctgca tggcagcctc cgtcattgtt ttggaagagg aaatcgcgga tcagtttgtt
      901 gaaaaattta aggctgcggc aaaagcgatc aagatcggca atggcttgga cgaaggggtc
      961 tttttaggac cagtgattcg aaaagatgca cagcagcgga cctttgacta tatccaaaaa
     1021 ggggtcgatg aaggagcgaa actggtacta gatggtcgcg aaaacattcc agaaaaaggc
     1081 tattttgttg gaccaacgat ttttgataat gtcacgatgg atatgaagat ctggcaggat
     1141 gagatttttg ccccggtcgc atcaatcgtg cgggtaaaag atttgatggc agccgttgag
     1201 ctgacgaatg aatctgaatt tgcgaatggt tcgtgtctgt ataccaacaa cgcatcagcg
     1261 attcgctact tcagagaaaa tgttgatgct gggatgctgg gggtcaatct tggggtgcct
     1321 gcaccgatgg cttggtttgc cttttcaggc tggaagcact ctttctttgg tgatttgcat
     1381 gccaatggca aagacagtgt tgagttttat acccatcgga aagttgttac agcacgttat
     1441 gcgcaaagtg agatttaaag atttgcttcg aagctctgtt aaagagcttc gaggattctg
     1501 ttataggcta taagccagag agaggttttt tctgtgaata ttcaactgaa aatgcctgcc
     1561 aatttaagaa ttggcggcgg aacaatccaa tttcttatgg aagaatcaga acaactaggc
     1621 aacaagccgt taatcattag tgatacggtt atggaacaga ccgggcatct tcaaaagatc
     1681 aaggagctgt tgggaaaaac agatgatcaa ttagcggagt atacagacat tcaagccgaa
     1741 ccgacgcaag aaaatgttta tgatgcgctg aagctttttc agaagcatca atgtgatcat
     1801 atcattgctt ttggtggcgg cagctgtctg gatacagcca aggtggtagc cgttttggct
     1861 gctaatcaat taagtctcga agcgattcat gcaagaaatt ttgatcagct gcaagcgggc
     1921 attccgctga ttgcagtgtc aacaactgct ggaactggtt cggaagtaac cgatgtggca
     1981 gttattacca atacaaaaac agcagtcaaa atgatgatga aggatctcgt tttcatgcca
     2041 cagatctcaa tcgttgactc tgattttacg aaaactgtac caccaaaagt aaccgcggca
     2101 acgggagtcg atgcgctatg tcacgctttg gaatcttatg tttcagtcaa agccaatgaa
     2161 gtaacgcaac tattttcact ggaagcgatg aagaaaattt tgaacacgct ttctcaggca
     2221 tatgaaaata gcgacgacac tgaagccaga gaacaaatga gcttagcttc aacggaggcg
     2281 gggattgcct tttcgaatgc gtcagtcaca ttgattcatg gaatgagtcg accggtgggg
     2341 gcactttttc acattcctca tgggatatca aatgcgatgt tattgccggt cttttttgaa
     2401 ttctcaaagg atgctattca gactgagtta gcgacgatcg ccagatttat ttatccggca
     2461 gccgaagatt tatcgctgga agaggctgcg gattatctga ttaaaaaaat ccaagagctc
     2521 tgtgagcagc tgtcgatccc aacgattcga gattatggta ttgatcagca agcgtttatg
     2581 gcagccttag acaaaatggc cgcggatgcg ttgaccagcg gaagtccgca aaataataaa
     2641 aaggtgccta gtcatagtca aattaaggaa ttatacgtac aagcttatgg gtagtaaaag
     2701 gcggagcaaa gagagggctc taccttttaa gaataaaagc tacacaggaa taattttgta
     2761 agcgcatact atgtgtgctt aaacaacaga acagggggat aagtaattga gagtaaaagt
     2821 aggcgttatc ggaacaggtg caatcggcat cgagcatatc gacaggatca atcagaaaac
     2881 acaaggggct tatgtgacag cagtctcaga tatcaatgta aaagctgcta aagaggttgc
     2941 caaacgtgtc gatgctaaat tctttgaaac cggagaagca ttgatcgctt ctgaagatgt
     3001 ggatgtcgtt gttgtgactt cttgggaccc gacccatgag cagtatgtgt tagaagcaat
     3061 caagcatggg aaatatgttt tctgcgaaaa gccattagcg atggatgcag caggctgccg
     3121 ccggatcgtt gatgcggaag ttgcttatgg gaagaaattg gttcaggttg gcttcatgcg
     3181 ccgctatgac cgcggctaca ttgaactgaa agaagcaatc gaatcgaagc aatacggaga
     3241 agcattaatg atgcattgtg cccatagaaa tcctagcgca gatgaaaact acacaacacc
     3301 gatggcgatc agcaacaccg cgattcatga gattgatgtg cttcgctggt tgctaaatga
     3361 agattatgaa tctgttcaaa tgatcttgcc gaaaaaaacc agcaactccc atgccgattt
     3421 gcatgatccg cagctgctga tctttcaaac aaaatccggt gtaacgattg atttggaaat
     3481 ctttgtgaat tgccgctttg gctacgatat taaatgtgat gtggttttcg aaacggcaga
     3541 agtaggaatg acggacccag cctatacacg aattaaatcg gaagaaaaaa attacacacc
     3601 tgtttcacca gattggcaga cccgcttttt ggatgcctat gattatgaat tcaagctgtg
     3661 gatcgattcg atcaaaaatg atgcggttgt cggtcctacc gcttgggatg ggtatgtagc
     3721 agcgatcacc atgatggctt gtcatgagtc tcgtgaaaca aaaacaaaag taatgattca
     3781 aatggatgaa cgaccagcac tatacaatga gtaattctca gaaaacggag gtatttcatg
     3841 aacaacatta gagtaggcat cgtcggttta ggaagattag gaaaagtaca tgcacaaaat
     3901 ttagcaacag gtgtcagttg ttgtgagctt acggctgcgt gcagtttagt tgaaacagaa
     3961 ttagcattcg cacagaagaa attaggcgtc aagcatactt tttccagcta tgaagaaatg
     4021 gtcaaaagtt ccgtgatcga tgcagtcttt attgtttcac catcaggctt tcactgtgac
     4081 caagtccgct tggcgatgga aaatggcaag catgtcttta ctgaaaaacc attaggcctt
     4141 gaagttgaag atattaaaaa gacccaaaaa gtcatcgaag agcatcctga acaggtcttc
     4201 atgttaggct tcatgcgccg ctatgatgaa gtcgtatcag tatgcgaaga aaatggtaga
     4261 agatggcgaa atcggcgaaa tcacactgat tcgttgttat gggatcgatc cgagcgaagg
     4321 cttggaaagc tttgtcaaat ttgccatgaa taacgacagc ggcggcttgt tcttagatat
     4381 ggcgatccat gatattgatt tggttcgctg gtttaccggc caagaagtcg atcaagtgtg
     4441 ggcgattggg aaaaatgcgg cgtatccaga attggatcag gctggtgagc ttgaaactgg
     4501 agccgcaatg atgagcctga gcgataaaac aatagcttta ctagttgccg gcagaaacgc
     4561 agcccatggc tatcatgtcg aaacagagat catgggaacc aaaggaatgc tgcgggtagc
     4621 gcaagcgcct gagaaaaatc ttgtgacgat catgaacgat caagggatca tccgtccatg
     4681 ttcacaaaac ttcccagaac gattcagaga agccttcatt cgtgaagccc aagaatttgt
     4741 ctcatgtatt caagaaaaac gtcaaccagc ggttaatgca gaagatggat tgcaatcaac
     4801 taagatcgcg ttggcttgtc aagaatcatt cgatacgaag agtttagtac aactaaaaaa
     4861 ataagcagga gtgtgagaat atgaccaaac aaattcaatt agccatcgca ccaatcgcgt
     4921 ggaccaatga tgatatgccc gagcttggta gtgaaaatac ctttgaacaa tgtatcagtg
     4981 aaatggccct tgctgggttc accggaacag agattggcaa caaatatcca aaagatccag
     5041 aggtgttgga acaatatttg aatattcgcg gcttaaaggt tgccagtgca tggttcagtg
     5101 cgtttctgac aactaaacct tttgaagaaa ctgccgaagc cttcaaagca catcgggatt
     5161 tcttacatgc gatgggcgct aaagtcatcg tagtagctga gcagggtcat agtatccaag
     5221 ggattatgac agcgccagtc tttgatgata aaccagtttt cactgatgaa gagtggaaaa
     5281 aactggcgga aggattagaa aaattagggg atttggccca cgaaaaggat atggagatcg
     5341 tttaccatca tcacatgggt accggcgttc aaacgacgga agaaatcgat cgtttaatgg
     5401 aaatgactga tccagacaaa gttaaattat tgttcgatac gggacatttg gtcttttctg
     5461 gtgaaaatcc aatcgcggtt tacaaccgtc atcaagatcg tatcaaacac atccatttca
     5521 aagatattcg tcaagaaacg gctgaagaag tagaaaagga aaaagacagc ttcttaaacg
     5581 gcgtgaagaa aggcgtcttc acggttcctg gtgatgggat gattgatttc actccaatct
     5641 ggacagcaat ccatgccagc gactatgaag gctggatcgt agttgaagca gaacaggacc
     5701 cggctaaagc caatccattt gaatatgcag tcaaagccag aagctacatt cgagaggtca
     5761 ccggtcttta atctattgaa aaggagctag acgatgaatc ttttttctat aatcagtttc
     5821 ctagtgattg tcatctgtgt atggttattt gcctattcta gatccagaag tgtcgatact
     5881 tctggatcag aagggttctt catgggaggg cggagcttaa ctgctttgcc aatcgccggc
     5941 acgatcatca tgacgaacct gtccaccgaa caaatagtcg gacaaaatgg tcaaagttat
     6001 catgcaggga tggaagtaat ggcctgggaa gtaactgcta cgattgcgat cgtagctttg
     6061 gctgtgatat tcttgccaaa atattttaaa tacggcatca atacaatctc agatttcatc
     6121 gagattcgtt atgatacggt taccaaacga attatttcga tcttatttat tgtgacctat
     6181 atgacttctt tcttacccgt ggttctttat tctggatcgc tggtgttcaa taagatcttc
     6241 catattgatg agctgctggg cgtccagccg atcgtcgcga tcataattgt agcgatggca
     6301 attggtattg tcggcatcct ctatctgctg atcggcggct tgtcgttaag tgccaatagt
     6361 gactccattt atggagtcgg tctgctggct tgtggtctgc tggtcccaat tttaggctta
     6421 atgaagcttg gtgacggcag ctttgttggc gggatcgaac acgttgtggt aaacacgcca
     6481 tggctgctta actctgtggg atcgatcgac tcggctgttg ttccatggcc gacactattt
     6541 acagggatgc tgtttaacaa tctgtatttc tggtgtacca atcaaatgat cgtccaaaaa
     6601 gcattgtccg gcaagaattt agctgaggca caaaagggcg ctttcctagt cggcgtcttc
     6661 aaaatcttag gtgcgttatt ccttgtcttt ccagggatcg tcgctcgcaa tatttttggc
     6721 gatagtttaa tgtcaaatcc agataacgcg tatccttttt tagtcagtga agtattgcct
     6781 aaaatgatct ttggtatttt cgccgctgtt atctttggcg cgatcttatc atcatttgcg
     6841 ggagctttga acgcgacatc gaccttgttt tcactggatt tttataaacc gcttatcaat
     6901 aagaaagcaa atgataagca aatcgctcgt gctggtaaaa cagtaacggt cgttgtgggg
     6961 attatctcca ttatcgtagc gccattcatt tcttttgcac cggctggatt gtatcagttc
     7021 gttcaagaat tcaatggact atacaacatg ccgctgctcg tgatcattct ctttgccttt
     7081 tattcaaaaa aagcaactgc ttttggggca aaaacgacga ttgccgtgca tatcgtcttg
     7141 tatgcactat caaaaatgct tttccaaaat atccatttcc tttatgtttt gacggcatta
     7201 ttcgttctgg acattatcat tttgatggtg gtttccaaat tgaaacctga tggagaattt
     7261 gtgttgaact cattcgatac aaaggtcgat atcgagcctt ggaaatacac caaggtgact
     7321 gctatagcat tggtagtcat tgttatttta acttatgtgg cattttcacc aattggcttt
     7381 gcaggctaaa aacaggaggg tattatgcat aaggatctat tgattatttg cgagaccgga
     7441 atcagtgcgt cgctgctcgt atcaaaaatg cttgaaacga tcagagccaa aaatctatcg
     7501 tatgagatag attatgcaac gatccgaaac gttgaagaaa aactgaatta taaagactat
     7561 gacgttttac ttttaacgcc gcaggtcgca cgttctgagg aaaaagtaaa agaaatcatc
     7621 aaaaaggaaa aatgtaccgc tcagatcgtt tatatcaaac aagaagattt tcgttatatg
     7681 aatattgaaa acatcattgc aagcatcgaa gaagattaac gagaatcttt gattattgag
     7741 tgtcgaggag gtggtatatg aaaagatggc atgttcgccc cgatcacccc aatatgaagg
     7801 gcggacttat tagaaatacc caatcttgta tttattcaat agggtaagcg gacctcaata
     7861 gccagctcat ccccaaaaag tcgaatggag acacttaaat gagtgtacct attcgacttt
     7921 ttgagactct aagtcaaatc gtgttgttct cattaaatca ctgaaaggaa atgcttcggc
     7981 gtttcctttt ttctttttgt ttttcttcgt gttttgaaaa ttacttacta tttactatac
     8041 gagtagtttc aaataatttt gacatcctaa tcttcaacaa cagaaaaaaa tctaaattat
     8101 aaaatttagg gttgactaaa atataatttt tgtataccat atgatcgtga taatcattat
     8161 catttagaag gagaaagtta tgttagaagt gaaaaagctt aatgtttgtt ataacgattt
     8221 tttagcattg aacgagattt ctttaaaaat acaagaaggt gctttaacag gtattattgg
     8281 tccaaatggt gctggaaaat ctacattgct taaagcaatg ttaaatatta tccctcatca
     8341 gggagaggtc tccattaata atgaagatat caataaaaaa ctggtgaaga ttgcatatgt
     8401 agagcaaaaa gctgatattg attttacttt tccaataaaa gtaaaagaat gtgtatctat
     8461 gggaacatat gccggaatga aagtttttca aagaattaaa aatgcagaat ggcaaagagt
     8521 ttcaaaggct ttggaaaagg tcgatatggg aaaatattct aatcatcaaa taggagaatt
     8581 atctggagga caatttcaac gtgtactttt agcaagatgt cttgctcaaa atgcggattt
     8641 catattttta gatgaaccat ttgtagggat agacttagtt agtgaacgaa taattatgga
     8701 tacgttaaaa gaattgaaac atcagggaaa aacagtgcta attgtgcatc atgatttaag
     8761 caaggtaaag aagtattttg ataatattat tcttttgaat cgaaatctaa ttgctcacgg
     8821 aagtgtagaa tctgtattta atgaagagaa tttaaaaaaa gcctatggag atactatatt
     8881 tattggagag gggggaacgg cttagcctct caatccaata aatatattat ctcaaaagaa
     8941 atgagttaat accaaattaa aataacgatg gttcataggg ttatccaatc aaaaccggaa
     9001 ggttaaaacc ctaactctaa tatacaaggg gaataatatg cttcaaaatt ttattaatgg
     9061 tctgtatgac tttcactttt tacagaatgc tctaattaca tctgtagtta tagggattgt
     9121 ttctggaaca gtaggttgtt ttattatttt aagaggaatg tctcttatgg gtgatgctat
     9181 ttctcatgct gttttgccag gagtagcaat ttcttatatt ttaggtatta atttttttat
     9241 tggtgcgatt gtatttggtc tattagcttc tatcattatt acttttataa aaagcaatag
     9301 tgtaataaaa ggagatacag caattggtat tactttcagt tcttttttag cattaggagt
     9361 tatccttata ggagttgcaa atagctcaac ggatttattt catatcttat ttggaaatat
     9421 tttagctgta caagatattg ataagtggat tacaattggc gtttctgttt tagtgttggt
     9481 tacagttgtt ctatttttta aagaattatt aatcacttct tttgatccat taatggcaaa
     9541 agcaatgggg atgaatgtca gtttttatca ttatctatta atgattctat taactttagt
     9601 agcagtaaca gctatgcaaa gtgtgggaac tatattaatt gttgcaatgt taattacacc
     9661 agctgctaca gcatatttat atg
//
LOCUS       DACDIH010000091         9522 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDIH010000091 DACDIH010000000
VERSION     DACDIH010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.091"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            33..656
                     /locus_tag="IV281_002424"
     CDS             33..656
                     /locus_tag="IV281_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781391.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /locus_tag="IV281_002425"
     CDS             1106..2155
                     /locus_tag="IV281_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8781392.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="IV281_002426"
     CDS             2152..3033
                     /locus_tag="IV281_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8781393.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="IV281_002427"
     CDS             3036..3806
                     /locus_tag="IV281_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8781394.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="IV281_002428"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="IV281_002428"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP8781395.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="IV281_002429"
     CDS             5117..6280
                     /locus_tag="IV281_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP8781396.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="IV281_002430"
     CDS             6397..6807
                     /locus_tag="IV281_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8781397.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="IV281_002431"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="IV281_002431"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8781398.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
BASE COUNT         3309 a         1617 c         2054 g         2542 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa ag
//
LOCUS       DACDIH010000092         9442 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDIH010000092 DACDIH010000000
VERSION     DACDIH010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.092"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..226
                     /locus_tag="IV281_002432"
                     /pseudo
     CDS             1..226
                     /locus_tag="IV281_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            352..1323
                     /locus_tag="IV281_002433"
     CDS             352..1323
                     /locus_tag="IV281_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8781399.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            1463..2776
                     /locus_tag="IV281_002434"
     CDS             1463..2776
                     /locus_tag="IV281_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8781400.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            2819..3685
                     /locus_tag="IV281_002435"
     CDS             2819..3685
                     /locus_tag="IV281_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8781401.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            3682..4530
                     /locus_tag="IV281_002436"
     CDS             3682..4530
                     /locus_tag="IV281_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8781402.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            4544..6016
                     /locus_tag="IV281_002437"
     CDS             4544..6016
                     /locus_tag="IV281_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP8781403.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            6033..6905
                     /locus_tag="IV281_002438"
     CDS             6033..6905
                     /locus_tag="IV281_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8781404.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(7141..8523)
                     /locus_tag="IV281_002439"
                     /pseudo
     CDS             complement(7141..8523)
                     /locus_tag="IV281_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            8985..9266
                     /locus_tag="IV281_002440"
                     /pseudo
     CDS             8985..9266
                     /locus_tag="IV281_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9340..>9442
                     /locus_tag="IV281_002441"
     CDS             9340..>9442
                     /locus_tag="IV281_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8781405.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQEL"
BASE COUNT         2890 a         1626 c         1968 g         2958 t
ORIGIN      
        1 tcttgaaaaa ttaattaaag acttagataa agccattgac gacttagtcg tggtgctacc
       61 ggagtatcaa tgtttaacca gtatcccagg tattggtcct gtttaggctg ctggaatact
      121 cgctgaaatt ggtccaattg aacgtttttc tgatgaaata aaattgacaa agtatgccgg
      181 cctatattgg cgacagagtc aatccggtaa atctgaatac gaaaattttt ttacgaaaag
      241 cttcacgtct aatatctatt ttaaaataaa aatattcctg ctaatttgga atcgtttcct
      301 tcttttggta taataatacc tgaaattctt ttgagaggag ttgttcgtga agtgaaaact
      361 actatgaagg atgttgctgc gttagcagga gttggtgtgg gtacggtatc tagagtaata
      421 aatggagtaa aggtgaaaga ggttaccaaa gaaaaagtaa tggctgcaat tagtgagctt
      481 aattatgagc ctgatgaata tgccagaggt atgaaaacga atagtagcaa tacaatagct
      541 cttattttac ctacgatttg gcatcccttc ttttccgagt ttgcctacta tgtagaggaa
      601 gctctcagcc aaaaaaatta taagcttttt ttgtgtaatg cgaacggcag tgcggaaaaa
      661 gaaaaagaat atatccaaat ggtcaagcag agtaaagtag acggaattat aggtattacc
      721 tattcagaca ttgataaata tgtatcatca aatttaccat ttgtcagtat agaccgccat
      781 ttttcggaac aggtttctta tgtaacggca gataattatt taggtgggca aattgccgct
      841 caagaactac ttactcgtaa ctgtaaaaac ttgtgctatg ttgggggtgt ttcaccttac
      901 cctaacgaaa caaacaatcg taaagctggt ttttacaata tttgtgagga aaataaaata
      961 gctgtgaagg tattggatat gcctgaacca attagcaatt tagaggaaca attgctcgct
     1021 tttttttcgg ataatcctga aattgatagc atttttacca tcaatgattt catggctttg
     1081 gatgtgataa aagcccaaaa aaaactaggg aaggaagcac ctaaggattt tcaaattatt
     1141 ggttttgatg gagtcaagct atcgttgcaa aaagactatt tactatcaac gattgtacag
     1201 ccgatagagg aaatggccaa ggtttcagta gaaacactca tggcgatgat tttaaatgaa
     1261 gaaccgccta agcgtgtggt agtaccggtt ttctttcacg ctggagagac gactaaagag
     1321 taaaaaaccg gtttataacg atttttttga gataggttga cagaaagtga tttcaatgat
     1381 atcttaaaac caattaattg gaaacgttac caattaattg aaaaataaaa cggaaacgtt
     1441 tcaaaaaata ggaggaacat cgatgaaaaa gagaatgtta gcaattgcag caattttagc
     1501 attaacagct ggaggacttg caggatgtgg cagtaaggag aaaagttcag gtagcagtga
     1561 tccaggagaa attaagattt gggttcagtt ttctgatgaa acgcctgaag gaaaagcttg
     1621 ggcagagatt gtgaacaatt ttaatgatga atacgagggt gaatataaag ctgttactga
     1681 gtatattcct cgtagcggaa gcggcggtgg atatgaggat aaagtaaatg ctgcaattac
     1741 aaccaatacc cttcctgatg ttattacgtt agatggaccg aatactgcag cttatgctca
     1801 ttcaggagtc attgctccat tagatgatta tttgaaagat gcggacatgg atgatgtatt
     1861 agacagcatt attcagcaag gaacctacga tggcaaaatg tatgcgtttg gtttctcaga
     1921 gtccagtgtg ggtgtctatt acaataagtc gctgttcaaa gatgctggaa ttacagaaga
     1981 tcaattgccg acgttagaag atccttggac ttggacggaa tttcaagcaa tttgccaaaa
     2041 gctgactgat aaatacaaaa cgcctgcaat tgatatgcaa atggcgtcgg gagacgaaat
     2101 gctgacctac gcatatacac catttatatg gtcgaatggc ggcgatgtcg ttaatgctga
     2161 tggaaccaaa gccgaagact attttaacgg taaaaaatcc gttgaagcgt tggaatttat
     2221 tcaagaattg gttgaaaaag gttacaccac aatcagtcct attgaaaaag gatttgaaac
     2281 tggcaaatat gccatgatgc tgagcggttc ttggacaatg gcagatttag aggaaaacta
     2341 tcaagagatt gattatggta ttttgcctta ccccgtttca gatgcaactg gggagttagt
     2401 ttcaccaacc ggaagctggc agttagcaat gactgagaaa acagagaaaa aggatgcttc
     2461 tgcggccttt attaagtttg caaccagtac agaatctagt cgtattatga gtttagggaa
     2521 cagtgttcta cctatcagaa attcaacagt tgatttaatt aaagatgaag tgtcggaagg
     2581 aatgcaggta ttaattaagc aaaaccaact ttctggacat gcccgcccag tggttgtagc
     2641 ttatccgcaa gtatctagag cattccaaca agcagttcaa gatattagct acttcgagga
     2701 aaacccagat gtacaaaaag tagcagatag tcgcgctgct gaaatgcaac aggcaattga
     2761 tacttcacta aaatagaagt ttctaaaggg ggccaatcaa gtgttaaaaa aaccgacaat
     2821 gaaagacaat ttgataggtt acggctttct agcgccagct ttgctgctgc tgacgatttt
     2881 tctgatcata cccgtaatca tggtaattta ttatgccttt actgattatt atttgttaac
     2941 gccagatgct agacaattta tcggcttaga aaattttaag cgactgttcg ctgatccaat
     3001 ttttgtaaaa agtatttgga acacggctaa atttgttgtt tggattattc cggtccaaat
     3061 aggggcagct ttaggaatgg cacttattat caacaaaaat cgtaaaggaa atatcttttt
     3121 taaagtagcc ttttttgcgc cagtcgtgat gtccttagtc gttatttcga ttttatggtt
     3181 gtacttgtta aatcctaatg aaggtctggt taatacgcta tttgcaaaga ttggaattgc
     3241 ttcgcagcct tttttgacta gtcccaaaca agcaatgtat acgcttgttt tcgtatcggc
     3301 atggcaaggt gcaggatatc agatgctgct gtttttaggg ggaatgcaaa atattccgca
     3361 agacgtttat gaggcagccg aaattgatgg tttttctaag tttcagcaat ttcgctatat
     3421 caccatgcca ttattaaagc ccacggcgat ttttatttta cttaccacat tgatttcggc
     3481 ctttaaacta atcattcagc caatggtaat gactcaaggt ggaccaatga attctactat
     3541 gacaatggtt tattacattt accaaactgg atttacagac cgtatggtag gctactccag
     3601 ctccattgcg ttaatcttta ccactatgat tggtttaatt acaattgctc aaagaaaatt
     3661 ggtaaaggag gatgatccag catgaccaag aaatttggac cgctaatgat cgtagaatat
     3721 tgtttgctgt tccttttagc ggcattattc attttcccga tgctgtggat ggtagtttcc
     3781 tctatgaagc ctgaagcaga tgtctatcaa aatttagctt ccttcaaagc ttttttacct
     3841 agtttgaatc cagctaattg gtttaaaact tatcaagagg ttatttcccg gttcagtgtg
     3901 ggaacttatt tattaaacag tattttttat ggtttaacct ttgcctgtgg gtcaatcatt
     3961 gtaaactctt tggcgggttt tgcctttgcg aaggttaatt tttcaggcaa gaaaattatc
     4021 tttggagctt tgttagctct gctgattgtc ccgatggaga cggttttaat tccgcaattt
     4081 accatcgtta attcactagg cttagtgaat actcgcttgg cagtgatttt accagcgatg
     4141 gccagcgcgt ttaatatcta tctgtttaga aatttcttta tcgcgattcc agaagaaatc
     4201 attgaatcag cgaaattaga tggtgcaaat attgttaaaa ttttctttca catcatgctg
     4261 ccaatgtcta aaccagccat agccacagta ggtgtcttat cctttatctc cagctggaat
     4321 gattatattt ggccgttaat ggtcttaact gacagcagca aattttcaat gcaagtggca
     4381 attactacga tcaatactac acagccagtt tatatcaatc aagtgatggc tgttttaacc
     4441 attgcaacga ttccactgat tattgtttat atcgttgcac aaaaatatat tctgcaggga
     4501 cttggcggtt ctggaacggg tataaaatag gagtgaatag tgaatgttgc aaaaactttt
     4561 aaaggccaat gagtttattg agaaaaatgc tcaaacagtc aatcaagaat accggaataa
     4621 atttcatttg atggctccga ttggctggat gaatgatcca aatggttttg tttattatca
     4681 agaggagtat cacttgtttt atcaatatta tccttacgac agcatctggg gaccaatgca
     4741 ttggggccat gctaaaagca aggatctaat tcactgggag catttgccag tagcactggc
     4801 accaggtgaa agctatgaca aggaaggctg tttttccggc agtgccatag aaaaggatgg
     4861 caagctgtat ttgatttata caggacacaa tgttgtggaa gggcaagttc gacaagttca
     4921 gtgtttggca gtttcagaag atggcattca ctttgaaaaa tatgccggaa atccaatcat
     4981 cgcagaagat cacttagctt ctgttgcaac gaccgaagat ttccgcgatc ccaaagtgtt
     5041 tcagcgaaaa gaagactatt ttactgtagt cgctactaaa actgcagatg ctcgtggaca
     5101 aattgtgatg ttccattcca aagatttact aacttgggag ttttattccg tattgctgga
     5161 gggaactcca gaacaaggca ttgtatggga atgcccagac ttgtttgaac tagatggcaa
     5221 agaagtcttg attctttctc ctattcaaat gactccacaa ggcaatgctt accaaaatac
     5281 gaactcaact gttgctttca tcggtactgt cgattggcaa acaggcaaat ttctagtaga
     5341 gaattttcat gaaatcgatg gcggtttaga tttttatgca ccccagacct gtgaaaacga
     5401 agcaggccaa agaataatga ttgcttggat gcaaatgtgg cagcgtaaaa tacctacgaa
     5461 tgatctgggc catggttggt caggttccat gaccttgcct agagagctga gggtggctga
     5521 taatcaattg ttgcaacggc cagtttcatc aatttatcaa gtaattgatc cgcaggaaag
     5581 gattgaatgg gcgaagactg aagagaatcg cttcttactg aaggacttag tatatgacca
     5641 gcactattta cgcttggaaa tcaatttaaa acaggcaacg aaactagaac ttcactatgc
     5701 gaagggaccc gaccatacat tactgcttaa ctatgatgtt gaacgggagt gctttactgc
     5761 ttccagggaa cacatgccgt atgatttatc aggagctgaa aaggaagtgt tgaaaaagcg
     5821 cagtgtgacg attcctttgg aaaataattg tctaaccatc gaaattttcc gtgatacttc
     5881 agctattgag ctttttctaa atgatcgtct gactatgagt acaacttttt atgagacaga
     5941 aaaaggacag gattttcttt tgctggcaga cagtgaagta accttttcat tggaaagcgg
     6001 cttaattaat ctatagaagg gagagctcag tgatgattta tggttcaatc gaagcaggcg
     6061 gcacaaaatt tgtgtgtgcc attagcgatg aagaaatgac gatcaaggaa agagtgagct
     6121 ttccgacaac gacacctgag gaaacaatac ctttagtcat tgacttcttc aaacaatacc
     6181 aagcagattt agcagggatt gggattggct catttgggcc tatcgatatc catcgcgatt
     6241 ccgccactta tgggtacatt acctcgaccc cgaaagtggc atggcaaaat tttgatttca
     6301 tcggtaccat gaaaaaagaa tttccgatcc cgatttcttg gacaacagat gtcaatgcgg
     6361 ctgcctatgg tgagtatgtc ttcggcagtg gcaaaggatt gagcagtgtc gtttactata
     6421 cgatcggtac gggtgttggc ggcggtgcct tacaagaagg gcgctttatc gaagggttta
     6481 gtcatccaga aatggggcat atgctcgtcg tccctcatcc aaaagatgac tttgctggca
     6541 gctgtccatt tcatggcaac tgtttagaag ggatggccgc tggacccgcc atcgaaaaac
     6601 gcttagggaa aaaaggacaa gaggttgcag aagatgatcc ttattgggaa atcgaagcct
     6661 cctatattgc tcaatgtgcc tacaatacaa ccttgatgtt ttctccagat gtgatcattt
     6721 ttggcggtgg tgtgatgaaa caacgccata tgctgcaaaa cgtccacgac gcatttgcaa
     6781 aattggtcaa tgggtatgtc gaaacacctg agttaagcaa atatatcgtg accccagcat
     6841 tagaagacaa cgctggaacg ctcggttgtt tggcattggc tcgagaagcg gtcttacatt
     6901 cataaacgaa aaaaggctct tcgacattta gtgttggtag agaaagatac aactgaaaat
     6961 ttagtcataa ccagttaggc agagtgctta actggttttt gttttacttg gaagatcaag
     7021 ccttgaatga gataagttct tgctcgaaag ttatggaaat ttcgatagcc gtaggctact
     7081 tgtattgtgc aatagttttt tacaaaaaaa agggggtgcg gatatttttt ttggaccttt
     7141 ttaacaaata tatctttgct tcttccgata ttgaagaggg cttagccttc ctaaagataa
     7201 cttaattcgt tgattattat accaatgaat ataatcatcc aatttgttta taaactgatt
     7261 gattgttgta ttcttccagt cacgttgata aaacatctcg ttttttaatc gtccaaagaa
     7321 accttcgcac gctgaattat ctggtgaata actcttttta gacatagagt gaagcaagtt
     7381 ggctttgttc caatagtcca actaacaata gcaccatcgt aacaatcgac aagtggtgat
     7441 aagtagattt tccctgcgag aattctaaat tcagtaatat ctgttaacca tttttcattt
     7501 ggcttctttg ctttaaaatt ccgatttaat aaattggggt gtgcaggaga aatttcccca
     7561 gcatacgaag aatacttttt tcgtctagaa aatttaacaa atagattttc ctcagtcatc
     7621 aatctacgta caactttttc agaaagcttt ttacccatat tttttaaagc ttggtgaatt
     7681 ctacggtaac catacgcaca gaagttatga ttgaaaatcg tgataattaa tttgacgttc
     7741 tttgtaatac ttatctggtt tctcaagtgc ttgtttttga taaaaatagc tgcttttagg
     7801 aaactggagc tgttgaagaa gctctttcaa ggtgaactga tttcgtaagg catcaatcaa
     7861 tatcgttttt tcttgatttg atagacaaag aagattgacg cctttttctt tttttagcag
     7921 ttcagccgct ccttcgagta atgctttttc gagctgtaat tggtgtactt gtttttgtaa
     7981 gtcttctact tcctttaaaa gtagctcttt ttgtttttcc tgatttttta aagtcataca
     8041 caatggaaaa tcctttccaa gaaccgtgtt tttccattga tatagagtaa ctctactaat
     8101 tcctccagat tcggctaaat ttttcctatt agtttgcggt ttatagaatt ttttcaacac
     8161 agcttctttt tgatcttgtg ttaactttac cgcacttttt cggagttttc tagctcctgg
     8221 tgcaagttct tcacaccgct gccgcaagga ctctctatta ggataaccaa gcatgcgaat
     8281 ggttcgtgca taacattgac catattcaaa ataatgattc accgctattc gtttttgttc
     8341 ttcagaatac tttgattttt ttcggtgctc ctgatggagc tctccggatt ccaaatactc
     8401 tttataccat ttccgtagtg caccaatcga cggataccct aattctcgaa tggttgcagc
     8461 ataagagcaa tcgtatttta aaaataactg aattgccctt acacgctctt tgtgggaaaa
     8521 cacacggaca ttcctcctaa gtttattttt tcgtccagga ttatgtccgc acccccttgc
     8581 tttctgatga cttattctgg ttctttgagt gtcaatgcat agatgtgatg tagctagtta
     8641 gaaaaaaatt ttgaatactc tttttttctt tcagactcct ttttaagtaa gattttgaag
     8701 tgtgagttac ggggtcagaa acttaaaagt ttttgatcct ttattttcaa gtctgatttg
     8761 gcagcttttc ggcagctcat ttatttatgg tcagaaatag atgctgaaat tttaatctat
     8821 tagcaaatag tccagaacgt atgaagagga gtcggatatc atttgtcatt aacaccataa
     8881 atatttataa aattcgtgtt cacctacaat taaatttaat atataatatt cgttgtaagg
     8941 gcttataatt ttttgttaat gaacaagata ggaagaaaca tttaatggac agttttcaga
     9001 gattgtcact agtaaatcaa tttgagttat tggctaaatt tgaggaccaa aacagatctt
     9061 attatgaaag aaaaatcgaa atattgagag aaggttacga gtatcattat gatgatgaaa
     9121 tttggagtga tctttctgag ccatttccga aagaagattc tcgatttgta ttagatgtat
     9181 tgaacatgta tcgagatatc aattttagta aatcaaagct aaacttagat gaaaaccaaa
     9241 ttactaattc atactatact catttcggtt ctgttgcaaa gttttccaaa aaatctattt
     9301 tagtgtaaaa ttgagaaaaa agacagagag gacagagtaa tgaatcattt taaaggcaaa
     9361 caattcaaaa aagacgtcat tattgtcgct gttggttact acctgcgtta caatctaagc
     9421 tatcgtgaag ttcaggaatt gt
//
LOCUS       DACDIH010000093         9136 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.093, whole genome shotgun sequence.
ACCESSION   DACDIH010000093 DACDIH010000000
VERSION     DACDIH010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.093"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(167..619)
                     /locus_tag="IV281_002442"
     CDS             complement(167..619)
                     /locus_tag="IV281_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781406.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(626..1174)
                     /locus_tag="IV281_002443"
     CDS             complement(626..1174)
                     /locus_tag="IV281_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8781407.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(1219..1551)
                     /locus_tag="IV281_002444"
     CDS             complement(1219..1551)
                     /locus_tag="IV281_002444"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781408.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(1565..1795)
                     /locus_tag="IV281_002445"
     CDS             complement(1565..1795)
                     /locus_tag="IV281_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781409.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKGYPYGPTN
                     LKNGKCRANWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(2017..2559)
                     /locus_tag="IV281_002446"
     CDS             complement(2017..2559)
                     /locus_tag="IV281_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781410.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(2580..3671)
                     /locus_tag="IV281_002447"
     CDS             complement(2580..3671)
                     /locus_tag="IV281_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP8781411.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(3679..5889)
                     /locus_tag="IV281_002448"
     CDS             complement(3679..5889)
                     /locus_tag="IV281_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781412.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(5905..8433)
                     /locus_tag="IV281_002449"
     CDS             complement(5905..8433)
                     /locus_tag="IV281_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP8781413.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(8485..8895)
                     /locus_tag="IV281_002450"
     CDS             complement(8485..8895)
                     /locus_tag="IV281_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781414.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(8908..9126)
                     /locus_tag="IV281_002451"
     CDS             complement(8908..9126)
                     /locus_tag="IV281_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781415.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
BASE COUNT         2846 a         1983 c         1279 g         3028 t
ORIGIN      
        1 tttgaacttt aacttttaaa aactttaaaa ataatcttat ttgttcttct aaacaatcat
       61 ctatactcat ataatcactc cactaattaa tctccccaaa aagacacagc attaagagat
      121 ataccaacac aacagtgcaa ataactctac atcttccttc gcaaccttat ttcaataaac
      181 cataaaaatt attttttaat agaaactgaa aattaaaact agaaaaagta gttttaccga
      241 atgtaagagc atcatcacca tcaatataga aatacattct tttccagtta catttaattt
      301 ttaaacaaaa ttctccccaa aaaggaactt tttctagtga aaacttggta atctctttgt
      361 aattaattct tgttaaagaa taattaaaca cttcattttt acttgttatt ttttcattga
      421 ctataactag ttcatctggt aaaaaaatta accaacaaac atcgacaaac agtaaatgat
      481 ataatccatt tccacctcca agcatattca atactggtga acatgcctta gaaacacgaa
      541 tatagttatg gtttaaatag atattttctt taaacattgc ttgtttaaaa gccatatgct
      601 cttgttctaa acttttcata aacttttact ctaatgcaca gctagcagca gctgctgcaa
      661 ttcctgctac aatatttacc ttagctaact ctttagctgc agcttttcct aattgttttt
      721 ctgcaatttt taataataaa tccgcagctt tttcaaaatt aaaagctttt aatgcttcaa
      781 taaaatctcc ctgcgtacct atagcccaag ccactcctcc tccaggaaga gaattaatgg
      841 ctacacatcc agcatatcgt ttccaatcaa ctttctccat tctaggaaca ataatatgtt
      901 cataaaagac tcttgctgct cttccttgtt cgttatcttg actgattcgt tcacgaatag
      961 caccaaaatc ctttatatgg tatacaccat gttcgtctaa aaaaccaact gtatcaaaat
     1021 aaaaagttaa ttcatctact aatttattct ttatttctaa atcaactgta ttcacttttt
     1081 ctgtggcata cacacttgtt ccagtactaa caaaagtact taaacctaat aagaaaacac
     1141 ctaaaacacc aaatatttta attattcttt tcataatttc tcctccataa aaaataagtt
     1201 actttttaaa tatcttgttt atgaattcat tccccaaagg ccttttaaga ttgatacagc
     1261 agtccgttca cttcttttag acttgtcata caagtctttt aaatgatccc atatcagact
     1321 agtttgtcta tctagcttca atccattaaa cgctaatgaa cggttcattt tttctattac
     1381 tttagataaa gctacagaaa tttttatacc ttcacaacgc gcatcttgga tttcaataat
     1441 agtatcatca attagttttt gaatttcttg cctatcaaca tattcaatct tagaaagaga
     1501 atcttttaaa gctactaatg attcaacgat ttcttttttc tcttcagaca ttttcttcct
     1561 ccttctagtg acccgtaaag ccatgtagag cagagtccat tgaaccagta attacactat
     1621 ctacaacgcc attccaattt gctctacatt ttccattttt cagatttgtc ggtccatacg
     1681 gatagccttt ttgacattca atgttcggat tataccagat aggattatac cattcagccg
     1741 cagaagcaga ttcagtcgat acaaacaatc ccacaccaac aaatccaata cacatcacac
     1801 tatagccaat tagttttttc atctcgtatc catctcctaa aaaatagaat agttcttata
     1861 gttattcata agacatattt tttccgtttt tgttttatcc tttctgtcct ataacaactt
     1921 ttttcaagta aagaatgaca tagtttttct taaaaaaaca gtctgttttt ttctatttct
     1981 tcggtgtata ataaatatga gtgttcattt tttattttaa tacgcactcc gtacataata
     2041 aatcttgatc gcatcaatct tttgagcttc ttgatctact ttcaattgaa gtaatacaaa
     2101 ttgagtaacc ttattcattg tttcttctcc aaataacaaa tattgatccg gattctctat
     2161 atcttgtgtc acctggtaaa gtttcccagt tgttttcatt tgtacatgcg ggtctacatc
     2221 aatactattg tctttaatac aacgatctgt catatagtcc ctcacagctt gattgcgttt
     2281 ataaacatct gtgaaattac tccattttct agcaaaatct tttaagaaat tttttgtcat
     2341 ttcatctatt tgcatttgtt gcgctgtctc gtcttctaat gaaccaattt gactgttact
     2401 ttcagtactt tgaagagttc cacttgtttc ttttactgct tcgattgttt cttctgtcgt
     2461 tctcaattgt gttttttgtt tttcagaaga aaaccaatga caaccagtaa gacctatcaa
     2521 acataaccaa ctaaatacta aaattatccc ttttttcacg tttctagtca ctgcctttct
     2581 tatttcacct tacgaaccag actacgaatt tttgtttgat tgtacgtgcg ttgataccga
     2641 gccataatcc gtccttgctc ggcattctgt tccacactag agaaatgttg tccgccatat
     2701 gaaacatctg ttataattcc ggtgtgacca tagattccag gagacaatgc accacctgct
     2761 tgccaattca ccacatctcc tgcttgtaaa tcacttggtt ttgggttcat gattacttgc
     2821 caaccatagg ttgtccagtt ataatcttcc cctattaaca tcgcaaacat gtgtccagaa
     2881 cccattaact gtgggccacc taattgagat acataccatg ccgtaccacc ataacattcc
     2941 ccattattta cggatcgtcc aatgatccga tccaaaacct tcgcattgcc tttacttttt
     3001 cctgcaataa gcgaagtttt ttttgttggt aggctattcc aatgcttgat cgcaaaagta
     3061 tcatattccg ttaagttata ggacgttatg atactcattg ctcctcgata atacggactc
     3121 ccttcggtaa tacctgctgc cgcatagcct ccacgaccta gaatcaataa acaacttcca
     3181 ggatctttat tgcccacaca ttgattaaag ttgctttggc tcgccaccat aaagccataa
     3241 tagtcataac aagcttttaa gttcggaaac ttcatataga aaccaccttc agaaccacca
     3301 attccacgtg cagaaccttg tgggtaggga caaccaggaa accaggtaat cccaaaaaag
     3361 ttcgtatcgt ttcttccaga ttgtgactgt ccataagctg attcatagcc taattgagaa
     3421 agaatcacac tcggtaataa ctgatacttt acagctgcac caactgctgc ttgcgcatgt
     3481 tcatctgtga ttggaccacg tcctttaatc tctgaaaaaa tcggtaatcc ttcggtatat
     3541 tccccattaa aagcgacagc tagacctgta gcttctcctc catgctcaga aagttctcct
     3601 gaaaaaaaga atggcaatag aaaaaaggga agaataaaga aaagaagaca acctagacac
     3661 cctttcttca tttcttcctc atcctttctt cgataatggt ttttggactg ttcgtccttt
     3721 tggtaaagta aaatcagttc ctggttttgc gtccaaaatc ggcagtgttt taggatcctt
     3781 ttctattttt tccgattgct ctgttggctt atctaaagaa gaaaatgcag gttgtaacga
     3841 ttttggtgcg gaaatcgacg gtttactttc agacgaacgt tgccccggtt gttgcacaga
     3901 acgatttcct gatagagtgg ctaccgacga attaggaaca gagtattggt tcaataactg
     3961 ctgcattgct ggataactcc catttttcgc ctgatttttc acttggcgaa tctgttgtga
     4021 aagagaacca gcagctgtta attgtggcac gggtactttt ccagaaggca acggaatcga
     4081 ttgaccaaca aaagcgatac ctttatttaa caatggtttt ctttgattat tcccttgttg
     4141 cttactcaat tgtgccaatg attgtacctg ttgttttggt cttactgaat tcatggacga
     4201 atttggaatc aaaccatttg aaagaccaga attccactct gttttcgatt tggtagaaga
     4261 catttcaggc tctaccccat gataacgaac atgacgacca ctataaggca aggcttgatt
     4321 tgaacgccca cctagttcaa agggattaac cacttgcctc ccttttaccg attcttgtgg
     4381 tgtacgtttt tgttgaaact tttgaaatcc atgtatcgct gcagaagcga gtggattcgt
     4441 tgctcgatac acattccctg ctaatcctgc acctttgatt gccaaatttt gtccagcagt
     4501 tgatagttga ccaggcagtt gtttcatttg agacaaagaa tgacttgcta tgtttttcgt
     4561 gttgtttata gtttgatctg cagctcctaa ccctagtttc tttaataaat ctttccgttt
     4621 tacaatgagt aaaattgata ggagtgcttt gacaaacata tttcctaaaa aaccaaaaac
     4681 agtattcatt ggaattaaat cgtctactat gttatagatc aaaaatgcca ccaaaataat
     4741 cacactgtaa aaagattttt gaaacatcaa gcccaaaaag gactttaagg tattcatgag
     4801 catataatca aatactggaa caaaagaagc gagtaatgaa aaaggaatca caagtaataa
     4861 acacaacacc aacatttgaa agataaaacg aatcatgcca atcactacaa gtggtaatcc
     4921 aatacttaga ttaagcaatg gcaccataga accaacgact gctttataaa cagctttagt
     4981 tggttgaata tagacacgat aagccttctt tttccctcta tcttctcctg cagcagtggt
     5041 cacttttgtc tcaatatcag ataattcttc acttgacgct tccttggcta gaaattcttg
     5101 tggttcaatc tctgcttgat acaattcttc taaatttgtt gtcccataat ttaacaatat
     5161 atacggatca acaacagccg ctttataata aagttggcgc actttttgaa tggcttcctt
     5221 ttgtgtcgtt ggcactaaaa caccattgac atcttttgtc gccaattcat ctagactatt
     5281 tgtagcagaa aaaataaagc cttctacctc cacggacaaa tcatttaacc ctttggtaaa
     5341 agcttctcct ttactaggta cgttactacc atagccaaac caaaccattg aaaaaatgac
     5401 aacgaagaga aatcgaataa acaccatttt tgcgtaatgt ttccctctaa atatacgaag
     5461 agtaaataga tataacacat acaagacgac aatccccgca ccaataggat cgtatatttt
     5521 tgaataaata gtatccgaaa aagtgaaaaa ctggtgaatt aactcgttca ttgcagctcc
     5581 ttcatagagc ttctcaatga caaaatcaag gccttgccaa atcaatttac ttaaactaaa
     5641 gaacatattc gtcatcattt ttagaaaatc tgtgccattt gccccaaata acattttgcc
     5701 taatgaaaat tcactatcag ataaataaga agaaaaggta tcaatatgaa atttcccaat
     5761 ctcatagatc gattgatcca cctgcttttc tgccgcatac accaatccac caacaaaaag
     5821 cagtccgaat ccaatcgtta aaaaaaacaa aacgcaccat ttcttttcct tatgctttat
     5881 ataagccatt gtttcacctc atttctattg catgattaac gtatcctgtt cttctacttg
     5941 agagtgactt gttttttccg ttgtttgtaa tgcagcggtc atttcttcaa acaaactgtg
     6001 tacattgagt tttcctacac gtccataggg atctaacatc caacactgtc ctttaatcat
     6061 gtggctcatt tcagctaagt tcgcttcatt tacttccaaa cgtaaatgat tcaaaatctt
     6121 ttctcgctca ctgtcttcat caaaagcaaa gacgacacca aaattccctt tttctttatc
     6181 tgaacctaag tcaccaataa attgtgtatc taacactaat tgattcatct gggaacgccc
     6241 cacacgtgcc attgctgcaa taatactacg tcctgcgttc gaattattaa atacccatgc
     6301 ttctgtaaaa taaatcgctg ttttcttact actatctcgt tttccaaaca tttcacaaaa
     6361 acgacctaaa ctaatcatca aacacaaaga ctttcgatcc gcatctgtat atagttctgg
     6421 tcggacattg tcatttggta atttcaaatc ttggatctct aaaatcgttg ttttggcatt
     6481 aaagtctaat cccgggtttt ctccataaga aaatcctaaa cgtaaaatcg aatcttctac
     6541 cgtcaagcgt aaaaactcac catattctcg aacctctttt tgttccattt gttctaaata
     6601 gtcgaccaca tgcatcaagc caacttgttg tccggcttct ttttgaacta aaacttcttg
     6661 gattcccttt aataccgccc cttttaaaag gtgattggta cctaacggac ttaactcatc
     6721 aatcatatcc tgggctactt gtttcgcatc tactcgggat aaacaaacaa ttggatctaa
     6781 tacaccataa ttacggggat ttgtagcatc taacgtgaca taactcattt tttgcaaatg
     6841 ttctacaaac aaagggtaat gtttctgata atacgggtct tgaatgagtt tcgtaaacca
     6901 tttgcgcttt tctcctttgg gatccacaaa taaactttgt acattcataa acgaactata
     6961 caaaaagagc aagccaacta aaaaggtctt ccctttaccg gtttttccag ttactgctac
     7021 atgaggcgca tcaaacaacg cacctttgat tccttccgca atggctagaa gattcaaaaa
     7081 gacaaaacga tttgacgcat aaattaattc ttctaatggt gttttttctc ctcgagaacc
     7141 accatcccct aatgtatctt gccgaccaat ataccagccg gattccatac ctaattctga
     7201 tactgctgaa aacaaccatt cgccaactgc ttctacagtt gttcggtgct tccaatgtaa
     7261 ctgccgtccc aatggttgcc ctaataaaca tttatagaac aattctcgtt gatcattttt
     7321 tgcacggtgg atatggataa tcgtatgaag ttgttgaata accgttgcac tattttgacg
     7381 aacttcttca accgtttgac cgtatacaat aaaacatgcg atccaatcga cgacaggttg
     7441 cccattatct actcgttgct ttaaatgttt tactaatcct cgaacagtta ataagcgttc
     7501 actaggttct tcatcatttt gaaccaattc cttatgctct gttttgaacc gttccgatag
     7561 ccaattcagt tttaaaccaa tacctccctg tcctttcaag cgtggatagt gaattttcat
     7621 tcgcaattct gctgggaaag aaagatcctg agcaaattga aatagatcac aatttctcat
     7681 attttcaggt gtttccccaa taggcaacaa tgctaaataa ctactcccta actcatcttc
     7741 taattgaaga aggcctttat gttccttcgg tatacgtaca tacgttcctt gattgtcttt
     7801 cagtacttcc caattatttt gttcttctac tttgtgagcc attcctcgaa tatattgcag
     7861 acgattgaga tacaacagct ctttttttac taagggacga atagaaaagc tataaagtaa
     7921 ttgtgtcact tcttttacat aaggatcact ttcagaaaac aaagtttcca aatcatctac
     7981 ttctaatcca aataagcgtg ctaaacttcc tgctaaatct aaccagatta gatttattac
     8041 atcatgaaaa ctctccaccg ttgctttttc tttgactttg attcctaaaa taaatgaagg
     8101 acgatagact tgaccttctc gttccaacaa tgtcactgtt tcttttgcat aatacgcacc
     8161 aattgcttga ctatcgggag aaaatccctt tcctaatgtg tcaaacttcg tccgtaaatt
     8221 cattgttcgt ggatacatca ccaactccac atcttcataa tctgctaaaa gcaacagtaa
     8281 acgagccatc atttctttta tttcctgtgt cttttttggt gtgttttttg aaattgttcg
     8341 tggaaaaaca tgatagtagg cccagataca atcctctcca tttaacaaac aatttttctt
     8401 gaatgctttt actggttctt ttacaagcac caatgaattt cacctcccaa taaaaaagga
     8461 acaaccagtt gattgtccct ttttctaatc ctctaaataa acatacttat tcacaaaaag
     8521 taattcttcc aatgcattgt ttgatacctt gtcccagtta taatacacac aatcaccatt
     8581 catcatacga atcatataac aaaaccaatc ccataaataa aaatgcactt tttttccatc
     8641 aggttttagt tttgccagtg ctaacacaca taaatagggg agtcctatat aaaaaacaac
     8701 tcctgctggt ggaaaaaaag aaatgaccca accaataaat gaacgtaaaa ataaatattc
     8761 aaagattacg accaacaaaa aagttgctgc attgactaat gcaactggaa atggaagacg
     8821 ccaattgctt gtaatctgtt gtacgcgata aggttccgca taaacaatcg aataatcata
     8881 aggttcccaa tccacttcac tatcctccta atccataaat aatttttcaa tgattttacc
     8941 aaacgcatca aatacacgtg ttggatcatt taagaagaaa taaactgcgg cacctgctgc
     9001 gatagatatt aatgcatttc ctacttttga tttcataaaa tttcgcaatc ccagataaac
     9061 aaccaccatt attaaaaaat agccaccttg cgttaatata aaatctacaa ttcccttcag
     9121 tgtcatttcc gttctt
//
LOCUS       DACDIH010000094         8764 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDIH010000094 DACDIH010000000
VERSION     DACDIH010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.094"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(365..2000)
                     /locus_tag="IV281_002452"
                     /pseudo
     CDS             complement(365..2000)
                     /locus_tag="IV281_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2555..3118)
                     /locus_tag="IV281_002453"
     CDS             complement(2555..3118)
                     /locus_tag="IV281_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8781416.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(3133..4176)
                     /locus_tag="IV281_002454"
     CDS             complement(3133..4176)
                     /locus_tag="IV281_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781417.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(4309..4713)
                     /locus_tag="IV281_002455"
     CDS             complement(4309..4713)
                     /locus_tag="IV281_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8781418.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(4946..5581)
                     /locus_tag="IV281_002456"
     CDS             complement(4946..5581)
                     /locus_tag="IV281_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP8781419.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(5847..6899)
                     /locus_tag="IV281_002457"
     CDS             complement(5847..6899)
                     /locus_tag="IV281_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8781420.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(6912..7850)
                     /locus_tag="IV281_002458"
     CDS             complement(6912..7850)
                     /locus_tag="IV281_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8781421.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(8301..8615)
                     /locus_tag="IV281_002459"
     CDS             complement(8301..8615)
                     /locus_tag="IV281_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781422.1"
                     /translation="MYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENII
                     LRTEYGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSH
                     RQ"
BASE COUNT         2830 a         1752 c         1352 g         2830 t
ORIGIN      
        1 tttctcagaa acaaaccact cattggaaaa gacatgaatc aaatcatcac ttacttgtgt
       61 aaaatagcga cgtttaatga caaagatatc ttcattgcta tctagtgtac cttcatttct
      121 ttgtgcttaa attcaaagat aattttctca ataaagattt ttgattccag taagattcct
      181 ttactccttt tacaacatag cgttgtcctt tgttttgaaa catcttcttc ccctctttgc
      241 cattaaaaat agcccaattc tctataaaga agattaagct tcattattat aatataaagt
      301 ttaaatttgg tttattactt actttttctt attctctagt aagctaatat tgaaaaaaat
      361 gtaatcagat cattattttt atatgttgta acttcctgat aagaaaacta attaaaagaa
      421 agaggtaact acataaacta attaaaaaag ctaacttcat agttggagga taaatggaag
      481 aaacttcaat gattgtttct ccttgtgttc cttcaatcga cactagtcct attatcaatc
      541 tctctttttt gtccatttac ttttgcgact gttccccaat aatcaaatac aggaagcgtt
      601 attttttgtc ctttagattc atgtactgta tagactattt tcgaatccat cacttgataa
      661 ggcatagtat gccattcctt gccaattctt acttcatgat tctgcaaatg ttctttgtat
      721 tgtaatgctt ccgtgggtgc atagtcataa agtcctccgg gaaggagtgt cgtgtaacga
      781 ataagatttt cttgattcat taccaagtga tctctctgag tgacaacttt atgttcatag
      841 tttgcttttg ttgcagcaga tacagccaac agtaataaaa caattgccgt tatattaact
      901 acttttcttt gcttagctga tgtgctcttc atgaaaacga acgataaaat caacgagcca
      961 aacagtactt gaaagcctaa gattcgccaa ggaaattgaa tcatttgaat gggtgtattt
     1021 tgtaataaag accatggaaa aaatttggtg gataagagcc aagagactac cgcactaagg
     1081 taaatcactt tttctttgcg attaaatcct ttaaaaaaga agagtggaca gatcaaagaa
     1141 ataataccaa tgattcctac cgtaaaggtt gtcaggtcat tattcatcgc accaatcaaa
     1201 ctatccgaga catttaatgc ttggtgttgt aaggtcgttt tatctggctc attaatcgat
     1261 tgaaccatga tttgtcggaa catcggatac caaaagtatg ctgttaaaag aatcgtcatt
     1321 cctactgcct ttgacaactc cacaatcaac cggatgcttt ctttttttct tgtttcactt
     1381 actacaaagc cgcctaagac tagtaagcca acggctatta cactcataaa agtggaaaga
     1441 acatgtgtat aaatgagtaa gctcatactg actgctaatg gaattgcaca tggctttttc
     1501 tcataaatca gtgcgtaagc ataatacata actatcggta taaccgtaag tgcaatcgct
     1561 tcagcaatag cagaacgatg ataaatatca atcgtacggt acccataaaa tgtatataaa
     1621 cagctaaata taaaagccgc atcgattctt tttaaaaatt ttagtccata atgaaacatc
     1681 agcatgatag tacaaacatt caatagccaa acatataaga tataactaac gattaaatta
     1741 ttacttatcc aataaaaaat gactgccgga aaaaaggtca agtagggata aaaataattc
     1801 actccgctcc cataatgatt aaacgtcgta aaattgattg gaccagaaaa aattgaagat
     1861 aaccctttga ctctagcaaa atggaaggca atatcccccg atgtagaaat agtatgaaca
     1921 tttctaaaat aattgattga ataaagtcca gcaataattg ctaggagaat ggacaatatt
     1981 atttttttct ttacttgcat tacttaatta tatactcctt tcatggattg ctttatctag
     2041 tataacttaa attttaaata aatcgttatt tatttttact ctcacttaaa aaagcactaa
     2101 aattataaga gagggaggta atggtctgaa caccaagaat tgttccaaca tcgaattgct
     2161 taactaatga gttcaaagct taattatgtg gctcttatat ttaccgagcg tggattgcga
     2221 tctctttagc gaaacaaaca tctttcataa aaaaaacagc catatccgct ttcttggata
     2281 tgactattta ctactacttt actatctttc attcttcttg tctcacttat gataaggcgc
     2341 tgcgtatcat gagtgaatga tattaggaaa acttcataaa ataaatcttt tatacatttc
     2401 cacttctaga tggtcattca tgttagaaga atcaaactat ttttttactg tcgtaataat
     2461 attaaaacac tagaacaaat caattaaatt ccataaaata taaataatag acctagcatc
     2521 atctggtata aaatggatgc taggtccgtt atatttactc aaaattgatt ctacgaatag
     2581 atcttcttat catttctatt ttagtagtca tgcgttgttg ttcttctaaa ggtggtaatg
     2641 gtataagtag tttagataaa tttgtaccat ttacatttgg ttgcccagtt ccgtaagaaa
     2701 ttttttctaa taacttccaa tataatggcg agtctaagaa gcagtctacg tattctgata
     2761 ataatttctc aactaaccta attcgaatca agtaagaagc aaaaacagat tcctcactta
     2821 tttccttaac caagaaactt tttcccatag ttcctcctgt ccttgctatc aaaatgtcat
     2881 tttcttccaa tcttaaatca actagtttag aattactaat atcacagtaa ggcacactgg
     2941 accaatttac acgtccctct tgtatatctg tgattctcaa aaacttaaca ttgccttgtt
     3001 ttttcgccga atctgtatag ccatattgaa tcttagtaga aatacttcct aaataaaccc
     3061 atagtcgaac atcgctagat gatttttctt gtcgttcttt atgaacaaca gtgatcatct
     3121 tgagttgtcc gactattttt tagttgcagc aatctttttg ggacgcttat ttgcatgaag
     3181 caacgttgct aagtctagta ataaaacatt ggctaacaca ggattggctt tctcgcgata
     3241 cgctgcggcg ccactttcaa acaatactgc tagacgatct tctacaacaa cgatttgttc
     3301 caaatacggt ggaaatttga ttcgttttag gatccggcta ggagagtccc gctcggacac
     3361 taagagttcc gaggtataag gaccataaga acgactggct aagagataat ggccataaaa
     3421 agccacacct tgaatcttat cagatgtttt tctgctcatt gtcacggttg gttggatttt
     3481 gtctttgcgg cgaattgctg ttttcatttc agctatcgtc ggcaatagat agaaagtcaa
     3541 ctgtccttca ccttttagtg tgaagttgcc agataccagt tgatcattgt tttgagtcaa
     3601 atacgatgct tgtggaatct ctgagagatc agttgtcagc caataagcga ttggctgtcc
     3661 cgtctcttca gatgtatctg ctaagatatc gatcaaacgt aaagcggcta ctcgtccagt
     3721 agcagatcct gtgattgtca accataaaag ttcccgtttt gtatcatata ctaagccccc
     3781 tacatgtggc cgtttaggta aaatcagcag tttgatcggc tgtcctgttt ctctatccag
     3841 tacatgaata atagaatgat gctgatggtc atagcagtag gcagaaataa acacaaaatc
     3901 ttgagaaaaa gccaaccctt gcggcgtcat ccattgacaa acctcatttt cgccggtttc
     3961 cggcaccacc attcttgttt ctgttaatcc aggaatgtta tattgttttg cttcagcctt
     4021 ttcccaagct gcttgaacaa taggataccg ttttaacttt ttttcaatat cctgatgtat
     4081 ccgttgctgc ttccaatgct taaactttag gacatagtaa acaagaaggc caatgaccag
     4141 caacactaaa atcattattg ctacccgctg tgtcattttt ttgcctcctt gttctattcg
     4201 atcaatcgtc catataccgc tattaattac cgtaactaca tgtttctttt actagcgttt
     4261 ctttatagga tctttcataa tctcatttat agtaacatta agaatccctt attctactat
     4321 agtcacttcc acatcaatat tgcctcttgt tgctttagag tatgggcaaa atgcgtgggc
     4381 ttcttctgcg attttctctg cttggtcctt ctcaagtcct ggcaaagaaa cttctaaaac
     4441 gaccccgatt tggtagcccc catcttctaa ttgaaataaa gatacccgtg ctttgacttc
     4501 agaatcagac tcaagattat tttcttccag catgtgttga accgcgccgt tgaagcaaga
     4561 tgcgtatcca gccgcaaaaa gttgttccgg atttgtccct tcactatgga ttcctgggcg
     4621 gtcgatcttc atgtgcatac ttccgtttgg tgcctcaact tcgccatcac ggcctccacg
     4681 attgatcatt gctgtttcgt acattttttt cattgagatc atcctttctt tcgtcgttct
     4741 atattaagtg taacgagtat tttttaatct gacaaaggta atgcttgcat tcggcgacat
     4801 cagttagaca acatcgatga tcagtgatag aagcagttta tctatacaaa aaaagaaggg
     4861 ttctatgata tttggtgtcc ttactcgcct tgagtaacaa caccaaatat catagaacca
     4921 cttaatcagc ttcacactgc tttttctact ttcgtagttg aaaaaaactt aaacaaatca
     4981 tcggcaaata gttaaagaaa aaacttaatc gttgaaagaa gcccaattgc ttaaagaagg
     5041 caaactgact aatcgacgga atccgtgcta atccgtaaag acctgcaatt agtaaactaa
     5101 taactgttaa aacaagataa aaatggctaa gcgtaccttg accactttga cgataaagca
     5161 gaacaagtaa caatgggaaa agtagaaagc ctgcatagcc tagcccagaa cctatattat
     5221 ggatccaagt cgatagatta caagagcttt cattcgtatt gatactgaat aagccagtaa
     5281 aaatacaatc acctatccca tataaaccaa ctgttgcaac aacaagtatt gcaagtgttt
     5341 ttgatgttcc aacaaatagg tgatacaacg caggcaacga aagaacaaaa aaaagtccag
     5401 atacaactga ccagaccaag aaagcacgtc tgactggact atctacgtca ccaaacacac
     5461 taattacggt agtcatttga ttcagtttgg gataaaaaag gccaaggata tacggtgtcg
     5521 ataaatcact catcacacct aataataaaa aataaaaacc atatttttgc aaaaaaatca
     5581 ttttcttccc ctacttttta gtgattattc gatacatcca acaatcaaat tttttctcat
     5641 tttaacacat atttttattc tagttgtctt ttgaacaatc aataacataa aggtgagttt
     5701 cttctacatt ttaaaaaatc gaatggcact gaagagttca gttgtcagca tcaatctcct
     5761 actcaaaaag gcaaaaaaac gtaagtcaga tgttgttgac atctcactta cgcatccatt
     5821 gattgaaaat ttcttagtaa aatctactag cctaaaaatt tgaagacttc ttctcgatca
     5881 tacagtttaa atactttctc gcataaaaat tgagtaatta gtgccgcaaa aacagggatg
     5941 actaaccaac aaagaacaac aagaaagcca tttagccctg catctaaaga agccaacgga
     6001 ccaacaagtc cgaccacgcc gaaaccagca gaagcaggcg tacctgaaat agaaaataga
     6061 gcgacaggaa tagctgtaat cactgcacta actaaacaag gaataagaat cacaggtttt
     6121 ctaaatagat taggcatcat catcttcata gcgcctaaag caatcgcaat tgtcacacct
     6181 gacttattaa ccttccaaga gttcactact aaaacaaaag tggttgcagc tacgcccatc
     6241 gcagccgcac cagcagaaac accatctagt tggattgcca ttccgattgc tactgttgaa
     6301 ataggagaga taatcaaaaa ggcaaaggaa caaccaatca agatagacat taacagcggt
     6361 tgtaaggtag tgaagttatt aatcaatgat ccaattgcag aagtaattgc tgttacaaaa
     6421 gggaataaat aaaaaccaag taaacctgca cctacaccta caacaatcgg tgttaacaca
     6481 atcgacactg acccaaaact atttccaatc agtaaaatca gcccgacagc aatcgatgct
     6541 gtcagcatcg tattaataat atcgcctgta ccagcaccaa cgtaaacacc cgtttcaccc
     6601 aaatcaggtg tgaatttgac tacaccagaa ccaacaaaag aagctccggc tacaaccatc
     6661 attctcatcg gatcaaatcc aaattgtaag gcaatcaaac caccaatgat caaaggtgtt
     6721 gctagttgaa aaataactgc taactgtgcg atcgttaccg caaagggata cgcactaaaa
     6781 tattttaaaa tggcacctaa tactgcatta ggaatcaatc caataatgat tcctgttgct
     6841 gtaccagcga ggactttatt cagaaaaatt ttcggtgata ttttttgtga agtttccatt
     6901 caatctctcc tttattttac gttcaaacta tcttcttttg catggacaag ctcagtcaag
     6961 aaatcacaat aaggcgtcgc aacaccatat ttcttccctt ttctagaaat tgcgccattg
     7021 atataatcga tttctgttaa tcgatggttc ttaatcaagt cttgatacat tgaaggataa
     7081 tgtaacccaa ttgtttccgg gtcaaaacaa gattcacaat gtgcaatgac ttctgggaca
     7141 tctagttcaa tcttctctac tgctgctact ttggcaaact cattgacaat cgtcgccacc
     7201 attttatgag cagtcgatgt ttttcctagc tcagccatat taacgtctaa gattgtgcat
     7261 agtccgttca ttgttccgtt cacacatgct ttgcgataaa tagaatagtg aatattgtca
     7321 gaaaaatgag catttaatcc tgattcagac aatttgtctg ctaatttttt tgcagctgct
     7381 tcttttccat cacctaaatt ttgtaattct actgaaccac ttccaaataa tttgacctga
     7441 ccaggacctt caagacctgc cgtccacatg gtatttccaa tatagatatt ttccattggt
     7501 acaaatttct caataatatc ttcatgcccg ataccattta atagacataa gacctctgta
     7561 tcttttttga ttaatgattg gatatcctgc agcatttttt ccagctgcat cgctttggta
     7621 aatagaataa tcagatcaac ttgatcttct ttttctactt cggattgaag aacgattggt
     7681 agttttgctt ctacctcttt accattaaaa tttgcttgta atccatgttc tttgatttgt
     7741 tggacatgtt ctgcccagcc atcgattaat aatacttcgt tcccactttg gtgaagcatc
     7801 aacccaaagc gacttcccat tgcgccagcg cctgcaattg ctattttcat tgattcttcc
     7861 tcctttttct ttctattata ctcatttgag aatcttttca caacctcgtt ttgttattct
     7921 tctcttattt atcatgttta tatccaccat tcaagttatt ttatcagcaa atcaataaaa
     7981 actggaatag aatcccaatt tcatcgtaag agaaactaga ctagcaataa gaaaatagaa
     8041 acattcatag aaaaaatctc actcctctga ttataaaaga gggggcatta acctcgtgta
     8101 tactaaaacc ttccattcat tcgatggatg attttctttt tgtgaaaaaa tattcattgt
     8161 agtacgatgt tgcgcaacac agacggatgg tattgcttat tatttattaa aatcgaacaa
     8221 tacacttatt taaaaacttc tttcccagat tttcttccat ttttacagtt ataactagta
     8281 ttatcatgaa aaatagcctt ttattgtcta tgggaataat aatttaaaat tggtactttt
     8341 ttattaatta gttcatcgcc caataatctc tccagtttct tagcatctcc tgactcaacc
     8401 ggaattatcc aagcccagtc taaataattt tttaaaatct catcatattt tgcaaatatt
     8461 tcattgccat attccgtcct taaaattata ttttcagtgc cagacaagtg atttatattg
     8521 agttgataaa aaagatctcc atctaaaaaa atatcattag aagaagattt ccccacagta
     8581 atgaccattc catcagctct atgtgttaca tacaaataat tgtatgacct catttcacct
     8641 aatgaatcaa ttatttcttg ccctttagct ctaatatatt gatctctatt acctatctca
     8701 ctcttattta acaaaagttt tttcatgtca aatttaatcg gctgttttat ttttgtaaat
     8761 tcgc
//
LOCUS       DACDIH010000095         8607 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.095, whole genome shotgun sequence.
ACCESSION   DACDIH010000095 DACDIH010000000
VERSION     DACDIH010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.095"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..456
                     /locus_tag="IV281_002460"
     CDS             <1..456
                     /locus_tag="IV281_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781423.1"
                     /translation="KIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHII
                     KHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVN
                     ANGLVQISGVKGVADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            631..1464
                     /gene="msrA"
                     /locus_tag="IV281_002461"
     CDS             631..1464
                     /gene="msrA"
                     /locus_tag="IV281_002461"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8781424.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            1544..2533
                     /locus_tag="IV281_002462"
     CDS             1544..2533
                     /locus_tag="IV281_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP8781425.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            2822..3520
                     /locus_tag="IV281_002463"
     CDS             2822..3520
                     /locus_tag="IV281_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP8781426.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            3670..4986
                     /locus_tag="IV281_002464"
     CDS             3670..4986
                     /locus_tag="IV281_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8781427.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            5003..6418
                     /locus_tag="IV281_002465"
     CDS             5003..6418
                     /locus_tag="IV281_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8781428.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            6882..7202
                     /locus_tag="IV281_002466"
     CDS             6882..7202
                     /locus_tag="IV281_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8781429.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            7228..7545
                     /locus_tag="IV281_002467"
     CDS             7228..7545
                     /locus_tag="IV281_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8781430.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            7574..>8607
                     /locus_tag="IV281_002468"
     CDS             7574..>8607
                     /locus_tag="IV281_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8781431.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLV"
BASE COUNT         2858 a         1386 c         1807 g         2556 t
ORIGIN      
        1 aaaataattt ctcagccggg acatattgtt gtaaacaaag aaacagtaga ggcgtatacc
       61 actaaaggaa tggattattt tttatatgat tattctccta tcgcttcatt ggagaataag
      121 gagcatataa taaaacacga agcaaagatt ggcagtcaat gggtaaacct tccttataaa
      181 acaactggta gttctatcat atatagagta actaatgaga agagccaagt aatcacactt
      241 cctgtatatg cttatttagg aacgaatgta gaaataaatc atcaacctgt aaaggagcag
      301 gtgaatgcta atggattggt acaaataagc ggagtcaaag gagttgctga tattaaagta
      361 agcacaagtt atacagtaac gactattatt tcttatcttg ttacattaac tgcatattta
      421 tgtttatttt ttaaaatagt tttccataaa aggtaaatta acttttcatt tataaaataa
      481 attgattaaa aaggagaatc tgtcgataag gattctctct tttttatacg aaaaatctgt
      541 agggggatga agtaatcgct tatttcctca ctttatgata aattacagct aaagtcacta
      601 gcttgtcttc ttcggaaagg aggagaatct atggatgagg aacaaatatt cattcaaaca
      661 ctttataatc tgattttgaa tccgaataca cgagattggg aacgaaaagt attgattcag
      721 acaaagaatg acataagaga aaatatatcc gttaaagaac agttatcaaa actagaagca
      781 acactacgtc cattagctat ccgaatgaat ctgacgcctg atgtaatgga cttttattta
      841 ttgattacgg aaggatttga taaggagcaa aaatatgact tttccaagca tgccatgcaa
      901 gatgctgact atcaagaacg tgcagtattt gcagggggat gtttctggtg tatggtcgag
      961 ccgtttgaat cgaaaaaagg gatcctatct gttttgtcag gatacacggg cggacatgtt
     1021 gaaaaaccaa attatgatca agtaagtggt ggttacacag gtcatgtgga agctgtggag
     1081 atcatttatg acacaagaga aataagttat agcgagttgc tgacaatcta ttggcaaatt
     1141 actgatccca cagatacatt tggacaattt caagatagag ggaagcaata tcgtccagtg
     1201 attttttatc aagtcgaacg acaaaaagaa ttagcggaac aatcaaaaca gaaactagac
     1261 agttcaggaa ctttccacca accaatcgtc acaaaaatcg aaccagccgg tacgttttgg
     1321 ccagcagaaa actatcatca gcaattttac aaaaaacaac cgaaacgtta taaaaaaatc
     1381 cagcaagcac gtaatcaatt tctgatctat caacgagtaa aaaacaaatg gcaaaaaaat
     1441 atacgaaaaa atcattttga ttaactcctt ttctttaaaa tgtagtaaac taaaaataga
     1501 aaaaagaaag cgttttaaaa agatttgatg aggaggagaa aaattgtata ttgcagatag
     1561 tgcacgttat gaaaaaatgg aatatcgtaa atccggtcat tcaggtttga aattaccgat
     1621 tttatctctc ggattatggc aaaattttgg tgattatgat cctatccata atcaaagaga
     1681 gattttaaga ggcgcattcg acatgggaat tacccatttc gaccttgcga ataattatgg
     1741 aggccctgct ggagctgcag agaaaaactt tgggcgtatt tttagagaag attttcaagc
     1801 ctaccgtgat gaactgatta tttctagcaa agcaggttat catatgtggg aagggccata
     1861 tggggaatgg ggctctcgta aatccatcat ttcaagttgt gaccaaagtc tccaaagaat
     1921 ggggctagat tatgttgaca tcttctatca tcatcgccca gaccctgata cacctttgga
     1981 agaaacagca gaagctttga tgcaacttgt aagacaagga aaagcattat atattggtat
     2041 atccaactat aatggagaag atacaaaaaa aatgactgaa attttaaaaa gaaaggaagc
     2101 accgttcatt attcatcaga tgcgctacaa tatgttttct cgtgcactac tagaagacga
     2161 tctatctcca gtattagaag aagaagggct aggggcaatc acgttcagtc cgcttgctca
     2221 agggttgctg acaaatcgtt atctacatgg tattcctgaa gattctagag cacatcgtaa
     2281 ggaaatacca ttcctttcag aagaacaagt tggatcaact ctggaaaaaa tcaaagcact
     2341 gcaaacaatc gccgtatcac gaggacaatc attagcgcaa atggcccttg catggaattt
     2401 gcgacaaaaa tctgttacaa gtgtactggt cggagcaagt cggttaagtc agttacaaga
     2461 aagtgttcga atgatggata atttagattt ctcaccagaa gaagaattga gaatcgacca
     2521 aattctagaa tagaaacgat gtaatcaaac tttttatcca tatattaaaa aaaacaaaag
     2581 atgacatcaa gaatcgaatg aagatcgtct gttattgaac atcatatttt ttgtcgttca
     2641 aaataaaaat gatcgaaggc taggagtgaa ttttatccta gtctttttta tggtaaatat
     2701 cttcttcgtt tgtatgtcta tgaacggatt aatcaatttt gtatacaaaa aatcatcaga
     2761 tgattcaaac gtgttataat aattacgaaa aaatacatca gatgaaaagg aggtatgtgg
     2821 tttggaagaa aataaaaccc tagtagaagt aactactgac aagatcctta gatatttaaa
     2881 ggaaatgcag ttgcaagtag gagataaact gccgaatgaa tatgaattgt ccgattattt
     2941 agaggtgggg cgaagcacgg tgcgagaagc tgtgcgggct ctagcttcca ggaatatcct
     3001 tgaagttcgg caaggagcgg ggacatttgt cagtgataag cgagggattg ccaatgaccc
     3061 actcggtttt tctttgattg aagatcagga tcggatgata cgagacttat ttgaactgag
     3121 atatttattg gagcctaaga tggcaggttt ggcagcggaa tatgcaacga agaatcaaat
     3181 cgtccaaatg aaaaaattaa cgcaaaagat tgaaacttct tttcgagaag gcacacaaga
     3241 acatgttcaa ttagacatcg attttcatac gatgattgca gaagctagcg gaaacgtagc
     3301 cttgctgcat attatcccaa tcattaatga atcaattgct ttgtttaata aaaattatga
     3361 tatccagcat ctgaaaatcg agacgatcga tattcataca gagatcgttc aggccatcga
     3421 aaggcggaat gcacttgctg cgtttgatgc aatgactgtc catatggcaa taaatcggag
     3481 agaattacag aaaataatag gagaaaaaga aactaattga cagttcatct gatgtatttt
     3541 atgataaata catcagatga atttttgttt tatatttggt aaaaaagagg agtaaatagt
     3601 ttctgttcat cgaaatgtaa gcgctgacta atagacaaaa agagatgacg tcaaggagaa
     3661 gaagcaggaa tgaggggatc tggaatgagt tttatggata aattagccaa tgttttaggg
     3721 aacgtggcaa cgagaatcaa taatttaagg tatatcatgg tgataaaaaa cgcatttgct
     3781 gcattgattc cggtaattat tacgggtgct tttggcacat tgttttcagc aatggtcttt
     3841 gactcggaaa atggcttagc acagataagt ttcttgcgtt ttttagaaga attaaagcct
     3901 atcgcatctg ccatcagcta tgtcacacta agtttcttga ccatctatgc tgtttttctg
     3961 atagggatcg aattagccaa gctgaataaa gtggatggtg tattccctgg tatcatcgca
     4021 gtgatgtctt atttatctgt taatccaact gtttatgaat tagtgaatga aaatcagact
     4081 gttatagtag aaaacgtatt ggctaaacaa tatacagata cgaaagggtt atttttggga
     4141 atgttggtct ccatcctttc tattgaactt tattgctggt tgaggaaaca gaagaaacta
     4201 cagctgagaa tgcctgatac cgtgccaacg aatgtttctg ctagtttttc tgctttattc
     4261 ccgacgattc tgactgtatc gggtatcgcg gctgcgggat ttatcatcaa agaacttaca
     4321 ggaatgtacc tttatgatat catttacaat gtcgtacaac gcccattaga aggtgtcgta
     4381 caagggttac cgggtatttt gctattgatg tttattgcgc aagtcttttg ggtgattggt
     4441 atccatggaa atcaaatggt gaagccgata cgggaaccgt tgcttttagc ggcaattgct
     4501 gtaaatacgg aagcttttga agctggaaaa gaagtaccga acattattac tatgccattt
     4561 tgggatatgt atatgagtat gggcgggtct ggagtcacga tcggtttact gattgcagta
     4621 ttgttagttg gaaaacggga agatatgaaa caaatcacca agttatccct tgctcctggg
     4681 atcttcaaca tcaatgaacc agttattttt ggtatgccga tcatgctgaa tccaatttta
     4741 gctattccgt ttatcttaac gcctcttatc actggaacga ttggttattt tgcaaccgct
     4801 ttaggatttg cggcgaaagc agttgtaatg gtcccttggc caatgccgcc gatcgtcaat
     4861 gcttatctgg caacagcagg aaatatcggt gcagttatta cgcaacttgt ttgtattatt
     4921 gtttctattt tgatctatct gccatttgta aaagtagcaa atcgtgtgga aaatgatggg
     4981 atctaaaaaa caggaggaaa tcatgaaaat tactattcca aaaaatttta ttcttggtgc
     5041 tgcatcttct gcttggcaaa cggaaggttg gaaaggaaaa aaagaaggac aggattcgtt
     5101 tatagatagc tggtataaaa atgaacattt tgtttggcat aatgggtatg gcccagccgt
     5161 agcaactaat tttatggaac aataccagca ggatgtggat ctgatgaaat ccatccatct
     5221 gacacattat cggacttcta tcaattgggc tcgttttttt actgattatg agaactgtat
     5281 agtagatgaa gaatatgctc agcatataag tgaaatgatc gactctttga tcgaagcaga
     5341 cgtagaaccg atgatttgtt tggaacatta tgaagtccca gcttttcttt tggaaaaata
     5401 tgatggctgg tcttcaagaa aagtagttga tttttatgct aagtatgctc aaattgcctt
     5461 tgatcgttac ggtgataggg taaaaaactg gtttacattc aacgaaccaa tcgttcccca
     5521 aacgcgttgc tatttggatg ctgttcgctg gccgcatgaa caaaacacaa aaaagtggat
     5581 gatgtggaat tatcataaag ttttagcaac ggctaaagca gtagagattt accgcagcgg
     5641 aaattatggt ggaaggattg gctgtatttt aaatcctgaa atggtctatg ctcgatccaa
     5701 ttctccagaa gaccaaaaag ctgcttatat gtatgatttg ttttataatc gagtattttt
     5761 tgatccgatg gtaaaaggat actacacaga agagttcctg gagttgtgtc aaaaacaatc
     5821 gatttatttc gatcctgcaa cagaggattt agaaacgatt cagcatcata cagtagattt
     5881 ccttggaatc aatcaatatt atcctaaacg cgttcgcgcc cctcgttatc aatggaatga
     5941 agagacacct ttccatcctg aacgatatta tgaagtcttt gatttacctg ggaagaaaat
     6001 gaataattcc aggggatggg aaatctatcc gaaaatcatg tatgatatag ccatgtatct
     6061 aaaagaaaat tatcataata tcccttggct gataacagaa aacggtatgg gaagagaata
     6121 tgaagaacag tatatggatg agaaaggaat cgtgcaagat gattaccgta tcgaatttat
     6181 cggacagcat ttgcattact tgatcaaagc aacagaagaa ggcgcaaatt gcgaaggcta
     6241 tatgttatgg gcattcactg actgtgtgtc accaatgaat gcatttaaga atcgatatgg
     6301 attagttcga atcgaattga acgatgaacg aagcagaaca ttgaaaaaat ctgcttcgtg
     6361 gtatcagact ttgattgata agagagaatt ggatttgcct gacgaaatct ataaataaat
     6421 agaagttttt tgtagatgtt cattgtattt actggttatt tgaaataaaa gaggttgtga
     6481 atccagcgtt ttgagctaat acccagggtc agctgattga acaccgttta ttgagaggtt
     6541 gtgaaaaagc tgtcctgcat caagtaataa gaatagcaaa gtaaaaacaa catcttatgt
     6601 ttttaacttg gttggactta ttaccgcaga tgcaagcttt tgaataccgt ttatttgaaa
     6661 aaagttgtga caagttttgt gtcacaactt tttttgtgca cgttaaaaga aaaaaggtaa
     6721 taaaaattaa tttgtgtgtt tagtgtgcgc tttctttttt agtgtgtatg caacttggta
     6781 tagaccttaa atgtgatgat atcaactttt taaagcgctt ttaaagttgg cacgctactt
     6841 gcttatatac aagtgtaagt atagaaagga acgtgtttaa aatggaaaag aaaacaatca
     6901 tgcttgtatg ttcagctgga atgagtacaa gtctcctagt cacaaaaatg caaaaggcag
     6961 cagaagcaaa aggaatggaa gcagacatct ttgctgtatc tgcttcagat gcggataatc
     7021 aactagaatc taaaaatgta gacgttttac ttttaggacc gcaagtacgc tttatgcaaa
     7081 atcaatttgc tgaaaagcta gcacctaaag gcattccttt agatgtaatc aatatggctg
     7141 actatggcat gatgaatggt gaaaaagtac tcgaccaagc agaaaaacta ataaacaaat
     7201 aataaaaatt atcggaggat acaataaatg gaagatcaaa aaaatctaga agccatcatg
     7261 ggtctgatca tgtttggcgg aaatgcaaaa agcgatgcaa tggaagcgat tgctgctgcg
     7321 aaaaaaggtg atttcacttt agcagatgaa aaaatcaaag atgcagagga atctttagtc
     7381 caagcacatc actctcaaac agagatgctg acacaagaag cacaaggaaa tcacgttgaa
     7441 gtcacgcttt tgacggtcca cagtcaagat catctgatga catctatagc ttttacagat
     7501 ttagcaaaag agatcattga tctgtatcgt cgattgaacg cgtaatctac agtaaagaaa
     7561 agagggaaga tttatggata aatttgttgc atttatggag aaacatttta tacctgtagc
     7621 atcaaaaatt ggtgcacaac ggcatttagt tgcgattcgg gactcattta tggtgagtat
     7681 gccgttaatg atcttaggag cattagcagt aatgatcaat aacttaccga ttcccgggtt
     7741 ccaagaattg atgaactcaa tttttggtgg tgaatcatgg aaaggattcg gggctgctgc
     7801 ttggaacggt acatttgcca ttctttctgt cttgattgct ttcttgcttg cttatcattt
     7861 agctaacggc taccgaaaag acggtgtttc cgcaggtgtg atttctttag gctctttctt
     7921 cgctttaggc ggagcattag gtatgagctc aaacggtttg tttattgcta ttattgttgg
     7981 gattatttca acagaaatct ttgtacgttt aagcggaaat gaaaaattga tcatcaaaat
     8041 gcctgatggt gtgccaccgg cagttgcaaa agcatttgct tcattactac cagcaatgat
     8101 cactatctct gcgtttgctt tagttgctgc aatttttgct gcattcggtg ttgatgatat
     8161 cgtaggttct ttctatacag tggtccaaga accatttatg ggacttgcaa actcttatcc
     8221 atctgcctta ctgcttgcat ttatcacacc attcttatgg ttctttggtt tacacggagc
     8281 gaatatggtc gatcctttga tgcaaacaat caatgcgcca gccattgaag caaacgttaa
     8341 tgcaatcgca gctggacata gcgcaccgtt tatcgtgaac aaacctttct ttgacagctt
     8401 tgtcaactta ggaggaacag gtgcaacttt aggtttactg ttagctattt atttagtagg
     8461 acgtaaaaac aaaccatata tggttgtaac aaacttgtcg attgcgccag gtgtcttcaa
     8521 tattaatgaa ccaacgatgt ttgggttacc tatcgtgttg aacccgatca tgttcattcc
     8581 atttattttg acaccaatgg tcttagt
//
LOCUS       DACDIH010000096         8342 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.096, whole genome shotgun sequence.
ACCESSION   DACDIH010000096 DACDIH010000000
VERSION     DACDIH010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.096"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..2206
                     /locus_tag="IV281_002469"
     CDS             <1..2206
                     /locus_tag="IV281_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP8781432.1"
                     /translation="TNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGI
                     NPGGFDGVYTPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSK
                     IRKMQQWLNVNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTS
                     KTYPVSEGNSGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTE
                     IADVVVIKGLLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGK
                     DERNKYLTTEELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIP
                     LTTVIYFAVDLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYA
                     SFAFVSDMSTGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNEL
                     SNDTNNDNYIAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIIS
                     SPVTEHDRKIYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTIND
                     SVSDFAGWLGDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYT
                     LAKRNSDSTALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDIL
                     GVGIISFFIDKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            2286..2681
                     /locus_tag="IV281_002470"
     CDS             2286..2681
                     /locus_tag="IV281_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781433.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            3006..3623
                     /locus_tag="IV281_002471"
     CDS             3006..3623
                     /locus_tag="IV281_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8781434.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            3664..3963
                     /locus_tag="IV281_002472"
     CDS             3664..3963
                     /locus_tag="IV281_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781435.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            3997..4164
                     /locus_tag="IV281_002473"
     CDS             3997..4164
                     /locus_tag="IV281_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8781436.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(4209..4814)
                     /locus_tag="IV281_002474"
     CDS             complement(4209..4814)
                     /locus_tag="IV281_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8781437.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            4939..7926
                     /locus_tag="IV281_002475"
     CDS             4939..7926
                     /locus_tag="IV281_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAP8781438.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPA
                     TRLGFALQLVSLRFLGTFILDLTNIPQNVLSYVATQLNIDPGEIIYYSRRQTRYQHTK
                     LIKSHYGYQDFNQPEIETYLANWLLNRALYTTESSDMLFDMLLKKCLDEKIVLPGITT
                     FSRFVASAIEKAEEHLYRQLSLVPTEDERKRLLNHLELIGTPVYGAIIKMDILRTPLA
                     DDSHKEIARGFDRLKQFQAFSTEKWKMESIPEGKIKVLANYAFKAKAQLIQRMSEQKK
                     LALLVAFIFIYKRKAMDEQILALSNFFETVFRRAKNKESKERLRTIKDLDRAASTLSD
                     IVDLVMSSHSDTNELSLRDRILEKYSEENVTEAVSQVRLLVKNDREPIAISELLNTYR
                     KFRKFIPDILTTLTFDSNDYGNNCKELWTFINNRFPKPITYKMFGAIKHTLPKKWIYY
                     IHEYPEQTNKCVLIAAVELLIQSLKRHDIYIPQSVLYNNPMDCLIDEKTWVQQKYVLL
                     EQLDLPSSANEMSLWLKNDLELSYKEALNRWPTSKMARIENADRLVVSRLKKTYEKKE
                     DKSFRRRIQQLLPKIDLSDLLLEVNQQLNLTQSFNHLSDKDTKMKDLDISLLAVLLSE
                     SCNIGFSPVSKEGFDSLKYDRLVYVNHHYIRVDTLSGANQKIIHAHRKLATSKIWGDG
                     HMASADGIRYITPQRSLYSRSNPKYFGRGRGITFYNFVSDQYIGFHGMVVSGTLRDSL
                     YLLEGFLNQPSELTPNQIMTDTAGYSDMIFGLFGLLGFQFSPRIANDKGTKLWRIDAN
                     AHYGELDTLSQNSINMELIYTHWEDILRVAGSLKSGKVNATELTKSLQRNGQPTPLGK
                     AITEYGKVYKTKHQLRYISDEIYARQILEQLNKGEARHALCRNIFYGRKGKLYQTYYD
                     GMEEQLNALGLVTNAIIYWNSLYLEKVFEQMKAEGFDCSEEMIRRQSPLISDHINFVG
                     KYTFRYEAELSNGKLRPLNVEEF"
     gene            8075..8284
                     /locus_tag="IV281_002476"
                     /pseudo
     CDS             8075..8284
                     /locus_tag="IV281_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346508.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2833 a         1286 c         1570 g         2653 t
ORIGIN      
        1 tactaatcta gtagctaatt ttggacctac cactgaaaaa atgtacgata atcaggttac
       61 tcctaaatgg ggcaaaaatc tacctaaaaa tattgtattt cttattcaag gtgccttttg
      121 gtgtaagggg attaatccag gtggcttcga tggggtatat actccatatt tagatactgc
      181 tgtaaaagag ttgcagactg atgcagggtt taatggaagc gctgtaactg gagttttgga
      241 tgcgaaatgg gccaaagcgt tatttgatat gagtgctttt gttcttgttc ccggcggaga
      301 tagtaaaatt agaaaaatgc agcaatggtt aaatgtaaat tatttagaat acacgggaat
      361 aatgccttgt gatggtattt atcaaagaaa ttcaaatcaa gcattaattt ttgcattaca
      421 agctgaacta ggatattctc ccagtgaagc aacagggtct tatggtaatg ggacaactag
      481 taaaacttat cctgtgtctg aaggtaactc gggaaactat gtaagaataa ttcaatatgg
      541 attatatgtt aatggttatt ttgaagacgg tacattcgat ggtattttca ctaaatatat
      601 gggtttagaa gtactggcat tccgaaaatt tatggtattg cctgaatata ctgaaatagc
      661 agatgttgtt gtaataaaag gactattgtc tagcgctgga gatattagaa gatctgcaga
      721 tggcgcagat acttctacgc aattaactag atctcaaatc caaacattgg ttgataatga
      781 tataaaaata gttggtagat atcttactgg tactgttgga attggaaaag atgaacgaaa
      841 taagtatcta actacagagg aattgaataa tattttttct aaaaatttat ctgtgtttcc
      901 tatctatcaa gatggaggtg cagcattagc ttattttact tatgatcgtg gcttatcaga
      961 tggtaagaag gctatagatg cagcaaaaaa tctaaatata ccattaacaa cagttattta
     1021 ttttgctgtt gatctagaca tgttagggga agaaattctt gcatatgctg gagaatattt
     1081 taagggggta tctgctgtaa tgtcatatag cgggtatcaa acgggtgttt acggaacaag
     1141 aaatgtttgt tctcaaataa tcaataacgg gtatgcttcg tttgcatttg tttcagatat
     1201 gtcgactggt tatagtggga atttaggttt tcctatgcct agacaatggt cttttgatca
     1261 atttgttgaa tttaccatag gaagtggcaa cggagctgtt ggtatcgatt taatcgctac
     1321 atcgggtaga gatagcggat ttaatgagtt atcaaatgat actaataatg ataattacat
     1381 tgccaaatac aatcagaagg ttataaatca gatggtacgt gcgtatcaat atctatcaca
     1441 agctggtctt gataatcctt ggaaccctca tttgacattt tatagatatg taaattattc
     1501 tggtttgtca tgggacatca tttcttctcc tgtaacagag catgatagaa aaatttatga
     1561 tgaatatagg aataaattaa ctaatagcga gggcctgtat aattatttta tagatcctaa
     1621 tactggttca ataataggtt tgcctcactt aattgtgacg ttacaatcac agatgtttct
     1681 agccgacaca atcaatgata gtgtttctga ctttgcaggt tggttaggag atttaatgac
     1741 ttgctggggt gaagttaaga aattaggaat acaaatggag caaggtgtat ttgcattagt
     1801 aggtacggct gcaggtggaa catttagtct agaagattta taccaagatg ttgacgcggt
     1861 aaatgtttat accttagcaa aaagaaattc tgattcaaca gctttatcgg ctttaaaaac
     1921 gtactattgt gataagggtg ctattgatag atttaatcaa tttgttaaga acagatttgg
     1981 ttcttcagat aaaatcgctt cagtaacaaa tagtttatta gaaaatgacg aaacagttga
     2041 tatcttaggt gtagggataa tttcattttt cattgataaa atagttaata attcacttgg
     2101 gacagatttt acttatcttg attttattgg agatgagggg aaagttgcaa atggattttc
     2161 tgaaaaaatt attcaatttc aagaaaatag aagtaaactt aattaagaat cgtttactat
     2221 ttaaacagtg tccttttcct ttttaaatat atacgataaa ataagtatat gaattgtaaa
     2281 aggacatgat gattatggaa gaaatagaat ggaataatct gactcaaaat aataaaaata
     2341 attctggatg tggaattatc tttatgtttg ttatagtttt gggaatagta gtacttttaa
     2401 taactgtcaa gatagtagat tactttgcga atactcctga agaacaaata cagagaaaac
     2461 ttcctgagtt tgaaagagta ccaccttatg atccaagaga taattcattt caagtaaaaa
     2521 aattaaaaga gtctccaaat gaaatgtggt ttgaagttac agttgaatca agcgacttca
     2581 aagaaaaaaa gaaagaatac aattatattg taaaggcaaa agtatcaaaa agatttattt
     2641 ttaaccctaa attagaaata tatagtgttg taatagaata atagtgttaa aaaaattgta
     2701 aaagttaaag aagtcaaatt ctgatgtgaa aaataaaata taccatggaa gacataaaag
     2761 aaaaactgga taactagttt atttaatggc agaccgttaa ggcctgccat ttttatgtat
     2821 tttagcggaa taaatataat catgcgggag tgtaaactta ctgtttcaaa aagtatactt
     2881 tttgacatgc cttgattttc tttgtttatg tatggcataa taaccacata atacaatttg
     2941 aaacgtttct gatttaatct gattgttttg aaacaatttt agtgaaaaaa gggtagataa
     3001 aaacgatgag aaaatttttt tatatccggg tcagtagtta tgatcaatcg atcgaacgac
     3061 aactgatttc cgcgaaggaa ttaggaatcc ctgaagaata tatatttgta gaaaaagcca
     3121 gcggcaaaga ttttaaacgc cctgaatatc aattaatgaa acggatgttt cgaaaaggag
     3181 atttactcta tctacaatca ttagatagac taggcagaaa taaacaaatg attttagaag
     3241 aatggcgaga attaactaag gaaaagcaga ttgatatttc tgtgttagac atgcctctgt
     3301 tagatactgc tagttacaag caactagatg ggttagagac gcttatctct gacttaattc
     3361 tgcaattact cagctatatg gccgaagacg agcggaaacg aattaatagc cggcaaagag
     3421 aaggcgtaga agctgcgaaa caaaagggtg taaaattcgg cagaaagaaa attgctttac
     3481 ctcctgaatt tcctgcgatc tattctgact ggaaagcagg aaagataact gcagtctatg
     3541 ctatggatca attgggcatg aaacgaaata ccttttatcg acgggtgaaa gaatatgaac
     3601 ataccttgca ccacttttct tgatagatat aacagctatt agacgggaag ggagagtttg
     3661 aaaatggact taacgaatca aagggtcgtt tttactggaa cgctgcagca aatgacacga
     3721 acccaagcca tcggtttggt tcactcttta aacggccatt gtcaatcaag tgttacttca
     3781 aaaacaaatt tcctggtttg tggtcagtat tctaagtcgt tgtttgatga ttctctctat
     3841 acaaaaaagg aacaaaccgc gcgggacttg attgcgttag gtcatgagat taccatttta
     3901 tcagaagtcg aattttatgc gttaattagt caacagttga aagaatggca acggtattta
     3961 taatcgttag taaaatttac aaggaggaaa atttttatga agcgaaaatc ttatgatcgt
     4021 tggggcattg aaataactag ttttaatcga aatattgctt ttcacgtgga gtgtgactgt
     4081 ggggaattag caaaaagtgt ttatcaaaaa aattactttt actgtcctaa atgccatcgc
     4141 cgatacgggg tacatggaac atagaaacga aatagtgcgg tagaaaagcg aatgaatgta
     4201 gtatatttct acaaatcatt cgctttcttt attttataaa tagttggtcg agagatcccg
     4261 ttttcttttg caatctctgc aactggaatg ccttgcttta acatgtccac cacggtttta
     4321 aaaactaagc gtttttgcgg atctgttgag ttaatagaat actcgattgg tcgtccctta
     4381 tatcggccgg ctttttgagc caataatact ccttgccgct gacgttcacg aattttctca
     4441 cgctcgtttt gtgcaatata acttagcagt gataaaaaca tatcgaacat agcggtatcc
     4501 aggagttcat ttccagtatt aaaatctaaa aactgtgcat ctaatatttt taacgctact
     4561 tttttctgtt tcataaaagc aactgtattt tttatatctt cataatctct tcctaaacga
     4621 tctagtgatg taacgatgac gcaatcacct tcacggatat aatttagtaa tttttgaaac
     4681 tcaggacgct cagtatcttt accactggct ttttcttgga ataatttatc aatgttaagt
     4741 ccttccatca ttgccaattg tcgatccaaa ttttgatctt gtgaagatac tcgaatatag
     4801 ccaatttttc ccataaaaaa ctccttttta aaagtgatat aatttacatg taaaatttac
     4861 ttgtagtttt actgctatca tagcattttt aaaaataaat tttatgtaga cttaaaattt
     4921 acttcatgga gggtttaaat ggatgaaatc aaaagtaaca aaaaagcatt tggcatttat
     4981 ttaggagaac ctagtacaga acaattaaac caattttttt atttgcatga aaaagattta
     5041 gaattgatgg agactatacg acgaccagct actagattag gttttgcact acagttagtc
     5101 tcactacgtt ttttaggaac ttttatatta gatcttacca atattcccca aaatgtattg
     5161 agttatgtag caactcaact aaatattgat cctggagaaa ttatctacta tagcaggaga
     5221 cagaccaggt atcagcacac aaaattgata aaatcacatt atggatatca agatttcaac
     5281 caaccggaaa tagaaacata cctagctaac tggcttttaa atagagcgct gtatacaaca
     5341 gaatcttcag atatgttgtt tgatatgctt ctaaaaaaat gtctggatga aaagattgtt
     5401 ttacctggaa ttactacttt ttctcgtttt gttgctagtg caattgaaaa agctgaagag
     5461 catttatacc gacaattgtc tttggttcca actgaagatg agagaaagcg gcttctcaat
     5521 catttagaat taattggaac tccagtttat ggggcaataa tcaaaatgga cattttgaga
     5581 accccgttag ctgacgatag tcataaagaa atagccagag gttttgaccg actaaagcaa
     5641 tttcaagctt tctctactga aaaatggaag atggaatcta ttcctgaagg gaaaataaaa
     5701 gttcttgcaa attatgcatt taaagctaaa gctcaattaa ttcaacgaat gagtgaacag
     5761 aaaaaactgg cgttgttagt ggcatttatt ttcatataca aacgtaaggc tatggatgaa
     5821 caaattttag cactatctaa tttttttgaa actgttttta gacgcgccaa aaataaggaa
     5881 tctaaagaac gtcttcgtac cataaaagat ctagatcgag ccgcatcgac actttcagat
     5941 attgtagatt tagtaatgtc cagtcattct gatactaatg aactatccct tcgcgatcgt
     6001 atcttagaaa aatattctga ggaaaatgtt accgaagctg tatcgcaagt tcggctattg
     6061 gttaaaaatg atcgtgaacc aatagccatt tctgaacttc tgaacactta tcgaaagttc
     6121 agaaaattta ttccggatat tttaactaca ctaacatttg acagcaatga ctacggtaac
     6181 aactgtaagg aactctggac cttcattaat aatagatttc ctaagccgat tacttacaaa
     6241 atgtttggag ctattaaaca tactcttcca aagaaatgga tttattacat tcatgaatac
     6301 cctgaacaaa ctaataaatg tgtattgatt gcagctgtcg aattgttaat tcaatcttta
     6361 aaacgccatg atatttatat tcctcagagt gtcttatata acaatcctat ggattgcttg
     6421 attgatgaga aaacttgggt tcaacaaaaa tacgttttat tagaacaact agatcttcct
     6481 tcttcagcaa atgaaatgtc tttatggtta aagaacgatt tggaactgtc ttataaagaa
     6541 gcgctaaatc gttggcctac ttctaaaatg gccagaatag aaaatgcaga tcgcctggtt
     6601 gtctctagac ttaaaaagac atatgaaaaa aaggaagata agtcttttag acgtcgcatt
     6661 cagcagttac ttcctaaaat tgatttgtca gatttgttat tggaagtaaa ccaacagtta
     6721 aatcttactc aatcatttaa ccacttaagt gacaaggaca ccaaaatgaa agacttggat
     6781 attagtttat tagcagttct tttatcagag tcctgcaata ttggtttttc cccggtttca
     6841 aaagaaggat ttgattcttt aaagtatgat cgtcttgtct atgtcaatca tcattatatt
     6901 cgagtggata cactttcagg agctaatcaa aaaataatac atgcacacag gaaacttgcc
     6961 acctctaaga tatggggtga tgggcatatg gcttcagctg acggtattcg ttatatcaca
     7021 ccacaacgtt ccctatattc tagaagtaat ccaaaatatt ttggccgtgg tcgtgggatc
     7081 acattctaca attttgtctc tgatcaatat atagggttcc acggaatggt cgtttcaggg
     7141 acactgcgag attcgcttta ccttctagaa ggattcctca atcaacccag cgaattaact
     7201 cctaatcaga tcatgactga tacagctggt tatagcgata tgatttttgg tctctttggc
     7261 ttattagggt ttcaatttag tccaagaatt gccaatgata aaggaaccaa actttggcga
     7321 atcgacgcta acgctcacta cggggaactt gatacgttgt ctcaaaattc aattaatatg
     7381 gaactgattt atacccattg ggaggacatt cttagggttg caggttcgtt gaaatctgga
     7441 aaagtgaatg caacagaatt aacgaaatct cttcaacgga atgggcaacc cacgccttta
     7501 ggaaaagcaa taactgagta tggaaaagtt tataagacga aacatcagct tcgttatatt
     7561 tctgacgaaa tatatgcacg gcaaatttta gaacaattga ataaggggga agctcggcat
     7621 gccttatgcc gtaatatttt ttatggacga aaaggaaaac tctaccagac ttattatgat
     7681 gggatggaag agcagctcaa tgcattagga ttagttacta atgccatcat atattggaac
     7741 tcactgtatt tagaaaaagt atttgaacaa atgaaagctg aaggattcga ctgttccgaa
     7801 gaaatgatac gcaggcaatc tccactaatt agtgatcaca tcaattttgt tggtaagtat
     7861 acgtttagat acgaagctga attatccaac ggtaagttac gtccattgaa tgtagaagaa
     7921 ttctagcaat gttcgggttt tgcatgctac cgcactattt cgtatgactg ttccagcgaa
     7981 tcacttcgaa tagaaaaaga gaaaaacacg agaacagcgt taaatcaacg tttcttgtgt
     8041 ttttactttt gtgctaccgc actatttcgt ttctatgttc catgtacccc tttatcaaga
     8101 agcgcaaaca cgccaaattg ccgattggtt gatcggcatg aacgcaagcc ctttatatac
     8161 cttgaattta caacaaaaag gcgtacaagg tacattttca ttaggacgtg tacaaacacc
     8221 gactttatat ctcatttatc aacgccagga agcaatagaa aactttaaaa aagaaccttt
     8281 tttcctgaat aattcatagt tttactaata gtctttccta acattgattt aatagtcttt
     8341 aa
//
LOCUS       DACDIH010000097         8229 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDIH010000097 DACDIH010000000
VERSION     DACDIH010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.097"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="IV281_002477"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="IV281_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP8781439.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="IV281_002478"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="IV281_002478"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP8781440.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="IV281_002479"
     CDS             2591..3376
                     /locus_tag="IV281_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8781441.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="IV281_002480"
     CDS             3389..4240
                     /locus_tag="IV281_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP8781442.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="IV281_002481"
     CDS             complement(4364..4816)
                     /locus_tag="IV281_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8781443.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="IV281_002482"
     CDS             complement(5281..5649)
                     /locus_tag="IV281_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP8781444.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5799..6281
                     /locus_tag="IV281_002483"
     CDS             5799..6281
                     /locus_tag="IV281_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP8781445.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6318..7328
                     /locus_tag="IV281_002484"
     CDS             6318..7328
                     /locus_tag="IV281_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP8781446.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7443..8156)
                     /locus_tag="IV281_002485"
     CDS             complement(7443..8156)
                     /locus_tag="IV281_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781447.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2784 a         1337 c         1667 g         2441 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
     5761 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
     5821 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
     5881 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
     5941 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
     6001 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
     6061 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
     6121 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
     6181 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
     6241 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
     6301 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
     6361 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
     6421 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
     6481 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
     6541 aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt
     6601 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
     6661 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg
     6721 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     6781 gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg
     6841 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     6901 gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt
     6961 taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     7021 cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg
     7081 ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     7141 cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat
     7201 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     7261 tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     7321 ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa
     7381 aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa
     7441 ttttaattat ccataactat cacattgatc agctgtttca catcatttgg attacctgta
     7501 ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct
     7561 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     7621 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     7681 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     7741 gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct
     7801 ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     7861 tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc
     7921 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     7981 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     8041 tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     8101 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     8161 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     8221 accttgtct
//
LOCUS       DACDIH010000098         8219 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDIH010000098 DACDIH010000000
VERSION     DACDIH010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.098"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            7..729
                     /locus_tag="IV281_002486"
     CDS             7..729
                     /locus_tag="IV281_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP8781448.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            787..2826
                     /locus_tag="IV281_002487"
     CDS             787..2826
                     /locus_tag="IV281_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP8781449.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2935..4086)
                     /locus_tag="IV281_002488"
     CDS             complement(2935..4086)
                     /locus_tag="IV281_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8781450.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4240..4770)
                     /locus_tag="IV281_002489"
     CDS             complement(4240..4770)
                     /locus_tag="IV281_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781451.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4869..5219)
                     /locus_tag="IV281_002490"
     CDS             complement(4869..5219)
                     /locus_tag="IV281_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP8781452.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5376..6905)
                     /gene="ahpF"
                     /locus_tag="IV281_002491"
     CDS             complement(5376..6905)
                     /gene="ahpF"
                     /locus_tag="IV281_002491"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP8781453.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6915..7478)
                     /gene="ahpC"
                     /locus_tag="IV281_002492"
     CDS             complement(6915..7478)
                     /gene="ahpC"
                     /locus_tag="IV281_002492"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP8781454.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7622..>8219)
                     /locus_tag="IV281_002493"
     CDS             complement(7622..>8219)
                     /locus_tag="IV281_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8781455.1"
                     /translation="EPGRKISEKALEEHLAIGRTPIREALIQLRQQELVYTIPQSGTY
                     VSKINLVSAENARFVREQLEEQIMIECCSKLDTQTRIVLETILEEQEKAVKINDERAF
                     FRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRWLRVTIQGLPWETIMEQHYDLLK
                     ALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYFQQ"
BASE COUNT         2773 a         1592 c         1496 g         2358 t
ORIGIN      
        1 tctattttgt tactgcttac attagtagtt ggctattttt cgattgagtt tatccatgga
       61 ttttcgtctg caaaacagag ttcaactgtg aaaaaggtag agccgaaaag tatccctacc
      121 acactaaatg tggctttgat tggttcagat gctcgttcga aagacgaaaa cggtcgctcg
      181 gattcacttc tggttgcaca atacaaccag aaaacacaac aagcaaaact gatctctatc
      241 atgagagatt catatgtcga tattccaggt tacggaatga ataagataaa tgcagcttac
      301 tcttatgggg gagttgattt attaaatcaa acattaaagg aaaatttcaa atttgaagct
      361 ccctattatg caagtatcac atttcaagat tttatcgatt gcgttaatga actgtttcct
      421 ggtggagtca agatcgatgc agaaaaatct ctagatttag atggcgtata tataaacaaa
      481 ggagagcaag tgatggatgg taatacttta ctccagtatg ctcgatttcg ggaagacaaa
      541 gaaggagact tcgggaggat cagaaggcag cagcaagtga tcaaagctat ctctcagcag
      601 ctgaaagacg taacttcaat attcaagcta cccaaagcgg ttgggaaatt acttggtagt
      661 atccaaacga accttccaga aagtgtgctt ctggattgtg gtatggattt cctcaaagac
      721 atatgttaga ataaacaggt ataaatagtg aaataaagga atgacaagca agagaaggag
      781 gaaaaaatga aaagaagtga caagcacggc aaaaatcgaa caggcgctta tattgccggc
      841 gcagtgatct taatagcagc tgcgggtggc ggttattttt actaccagca ctatcaagaa
      901 acccaagcag tagaagctgg agaaaagaca gttgagcaat ttgtccaagc tttgaacaag
      961 ggagattata acaaagctgc agaaatgaca tcaaaaaagg cggcaaataa aagtgcatta
     1021 tctgaaaaag agatcttaga taaataccag aatatatacg gtgctgctga tgtcaaagga
     1081 cttcagatat cgaatctaaa agtagataaa aaagacgatt ctacttacag tttttcatat
     1141 aaagcaaaga tgaatacctc attaggtgaa ttgaaagatc tttcttataa aggaacatta
     1201 gacagaaatg atggtcaaac cacgatcaac tggcaaccca acttggtttt tccagaaatg
     1261 gaaggaaatg acaaagtaag cctgaccaca caagaagcag caagagggaa tattatagat
     1321 cgaaatggtg aaccattagc aacaacaggc aaactaaaac aattaggagt cgttccaagt
     1381 aaacttggag atggaggcga aaaaacagcc aatatcaaag cgattgcttc ctccttcgac
     1441 ttaacagaag atgctatcaa tcaggcgatt tcacaaagct gggtacaacc cgattacttt
     1501 gtcccattga aaatcattga tggagcaacg ccagaacttc cagctggagc taccattcaa
     1561 gaagtagacg gcagatatta tcctttgggt gaagcagctg ctcagctgat tggttacgtg
     1621 ggagatatca cagcagaaga tattgataag aatccagaat taagcagtaa tggaaaaatc
     1681 ggtcgctcag gtttggagat ggcttttgat aaggatcttc gtgggacaac aggtgggaaa
     1741 ttaagcatta cagatgcaga cggtgtagag aaaaaagttc tgatcgaaca tgaagttcaa
     1801 aatggcaaag atatcaaatt gacaatcgat gcgaaggcac aaaaaacagc ttttgacagt
     1861 ctaggaggaa aagctggatc aactgttgcg acaacgccaa aaaccggtga tcttcttgcg
     1921 cttgctagct ctccaagcta tgatccaaac aaaatgacaa acgggatctc acaagaagat
     1981 tacaaagctt atgaagaaaa tcctgaacat ccattcatca gccgatttgc gacaggttat
     2041 gctcctggat ctacgtttaa aataattaca gcagcaatcg gtctcgacaa cggcactatc
     2101 gatccaaatg aagtgttgac gatcaacggg cttaaatggc aaaaagacag ttcttgggga
     2161 tcttttcaaa caacgcgtgt cagtagtgat gtatcacaag tagacttaaa aactgctttg
     2221 atctattccg ataatatata tgcggcacaa gaaacgttga aaatgggtga gaaaaaattt
     2281 cgtacaggct tagataaatt catttttggt gaagaccttg atttgccaat tagtatgaat
     2341 ccagcacaaa tttctaatga agatagcttt aactcagata tcttgctagc tgatactgga
     2401 tatggacagg gcgaacttct aattaatcct atccagcaag cagcaatgta ttctgttttt
     2461 accaacaatg gcacacttgt ctatcctaaa ttgattgcag ataaagagac aaaagataag
     2521 aagaatgtca tcagcgaaac agcagtacaa acgatcgtgc cagatctgag agaagttgtg
     2581 caagatgtaa atggtacagc acattctctt tctgctttag ggattccgtt ggcagcgaaa
     2641 actggtacag cggaaatcaa agaaaaacag gatgtaaagg ggcaagagaa cagtttcttg
     2701 tttgctttca atcttgataa ccaaggttat atgatggtca gcatgttgga aaataaagaa
     2761 gatgatgatt cagcaaccaa acgagcatcg gaactattgc aatacctcaa ccaaaattat
     2821 caataaaaaa ttgcttttta agtacagtaa aaggactgtg gcgttatcgt cacagtcctt
     2881 ttttcaaata aacggttttc acgatgtctc tttcacaact tctttttgat ttactcacac
     2941 ttcataaact ttttttgtaa aatatttgag aacaagtgga gaaataagtg tacttaataa
     3001 aatcacgata actaaaggtg aatagtactg tggttcgatc aattgacttt gctgtccaat
     3061 ttgtaaaatg atcaaagcca tttctccacg cgaaatcata ccagcaccga ccatcaatga
     3121 actatgttga gaaaaaccgg caatctgagc accaaaataa cctccaagca atttggtcaa
     3181 aatcgctaca agcgtcaata tagaaataaa gagtagttgt tcactaaagt cagaaaaatc
     3241 aacttctaat cctacactga caaaaaatac tggtataaat actgcatatc ctaaagcctc
     3301 tacattgtgg tatacttctt ctttaacctt tgtctgactg acagctatcc cagcaaaaaa
     3361 agcaccaatg acagagctaa gcccaacaag atccgctacg taagacattc ctaaacaaat
     3421 aactagcgat acgatgataa tagaagaatt tgcgtacact ttttctgcaa atgaaagtag
     3481 gaaaggcgca atccatttga ctaataagaa aataaaaaca aaataaatta gctgttcaat
     3541 caaaacaagc ggtaaagaag cattgctaga agcttccccc gttagaaaag ataggctgaa
     3601 acttagaact aaaacgacta aaatgtcatc taccacagaa gcacccaaaa ttgttgatcc
     3661 ttcttttgta ttcactacgt ttagttcttt taatacttct actgaaatac tgacagaagt
     3721 tgccgcaaga ataatcccaa agaaaatcgc ttcatttccc gaaacttgga atgctttgcc
     3781 tgtcaaccag ccaaaaacaa ccgggaagat gatcccaagc aaagcaacga acataccagg
     3841 tcggaaatat tttttcaaaa gagataaatc actttccaga ccagctaaaa acattaataa
     3901 gatcacacca atctccgaaa agtcatggac tagaatatca ggtttgaccc aattcaatcc
     3961 agcttggcct agcaaaacac caacaagtaa ttgtccaatc actgctggga tacctaatct
     4021 ccgcgagata tgtgagccaa tcgttgttga aatcaatatc aggcataaaa taccaataaa
     4081 ttccattctc atcattcctt tgtataaaat atagcccgaa cttagcataa catacattat
     4141 tgaaaaaata ggaaaatata ctcacaaaaa aaccaacaat ccaaaagatt tttttggatt
     4201 gttggttttt atacggtcta ttttaataac taaaaatgat taacgtatag tcatttttct
     4261 tattctgatg aaattgataa tctcctggat gcccatgaaa atcaaaataa agccgaatat
     4321 ttggaataga agcatcaaac ttttgaatgg attgaacaat aagacgaatc cgccaatcaa
     4381 caacagaata ctactataaa cactccaact tttagctttt gtattcatgc tgaagaacaa
     4441 ctggaacaag ctgttgatga taacagctaa tccaagaaga atcggcagga tagaaacgat
     4501 tgcaggtgca aaagcataga cgatcagagc taagatcagg aaaaaaagac cactgaacag
     4561 accgatactc caagaacctg ttttcctttt aaaacggtaa gcctcaaaaa gattcaacaa
     4621 cccaagcaaa acaagatagg cggtgatcag ataaccgata aaatgaaaaa cagcagaagg
     4681 gtttagcaca atcgcaatcc cagaaataat ataaacagca gctcttaata aggcgtattt
     4741 ttgaatattt tgtttaatcg tctcaaacat ataagaaaac ctcctttgac ttattttaac
     4801 gtgaactagt cattttacca aattaaatgt tacaaaatga gcgaaggaga agaatatctc
     4861 tggaaaaatc aacttctgag gatcttttcc atttttttgc cttttgctag ctcatcgatc
     4921 attttatcta aataacgaat ttctttcatc aagttatcct cgatgtcttc tactcgtaca
     4981 ccacaaacaa cacctgtaat caaggaacgt gaaggattgg ggacaggggc ttccgcaaaa
     5041 aattgttcga aagtcacttg tttttgtagt tgatattcta gttctgtttg ggaataaccc
     5101 gtcagccaac aaatgatttc atctacttcc tcttttatac gatcttttct ctccgctttt
     5161 gctacataca taggataaac gcttgcaaaa cttgtcgtaa aaatacgatt ttgcgccata
     5221 atcagtcacc tttctttttt tcaaattatt tactacttat agtatataag aaaaagagaa
     5281 gaaaaagcga ttattcaaaa aaacaaaaag gattgtgaca aaattcttgt caccatcctt
     5341 ttttccgctc tctgactttt tacaatcttc atagctcaag tgcgcatcag ataatcgaat
     5401 gctcctaaag ctgcagtagc tcctgaaccc attgaaacaa tgatttgttt gtatggacta
     5461 tctgtacaat caccagctgc gaaaacgccg gggatattcg ttgcgccatt tttctcggtc
     5521 actatttcat ttcttggatt catttcgatc gttccatcta gccaatccgt attagggagt
     5581 aagccgacta gaatgaatac tcctgaaaca gctagtgtat gcatctccat cgtacggcga
     5641 tcgatatagg agagagattc tacctgattc gtaccgctga tttctttcgt cgctgcatgt
     5701 ttgataacag tgacattcgc aagagagact agtttatcct gtaagatttg atctgctttt
     5761 aattctggta agaattctag aacatataca tgttttgcta acccagccaa atcgattgct
     5821 gcttcaacac cagaattacc accaccgata acagcaatct cttttccttt aaataaaggg
     5881 ccgtcacagt gtgggcaata agcaattcct ttatttttaa actctttttc gcctggtaca
     5941 ttgatagaac gccagtgcgc acctgtcgcg atgatggcgg tttttgcttt aagtgatata
     6001 ccgttagcta agccaatatt aataaaatgt tgctctttat ttaaggaaac ggcctgttga
     6061 ttcatcataa tgtcgatagg gtaactacgc acatgacttt ctacttgctg catcaatttt
     6121 ggaccttctg tgtaagaggt accaatcata ttttcaatcc ctagtgtttc aacgacttgt
     6181 ccgccgaaag aatcagcgac aagtcctgta cggattcctt tacgtgctgc atagatagct
     6241 gcactactag ctgctggtcc accgccaatt actaatacat cataaggttc tttttcaaac
     6301 aactcttctt caatcaaagg gccagtgatt ttttcaacaa tttgttcgat cgtcatgcgt
     6361 ccgctactaa attcttctcc atttaaaaac acagttggca ctgccatgat ttttttatca
     6421 tcgatttctt tttggaacat cccaccttca atcatcgtat gagtaatatt tggatttaat
     6481 acagctaata tattcaatgc ctgtacaacg tcggggcaat tatgacaagt caagctaaca
     6541 taactgtcaa aatgcaatgt ttgtgttatt tttttgattt taagactcaa agattcactg
     6601 attttcggtg cccgtccact aatttgtaac atagccagta aaaaagagga aaattcatga
     6661 ccgaggggga taccagcaaa aacgattcca ctttctttgc caactggatt gatagaaaaa
     6721 ctaggtgtgc gcatcgaatc tcttttttcc caagagattt taggagacat ttctgctatt
     6781 tccttcgtaa attcagctag tttttgacta ttttcactat tatccaaact aacagaaaaa
     6841 acaataggag attcaatcag tgctaaatat tgttcgagat tttgtttagt ttctttgtct
     6901 aacatatcca ccacctaaat ctttccaaca agatcaaaac ttggtttcaa tgtttcgcca
     6961 ctttctttcc attttgccgg acaaacttca cctgggtggg tacgtacata ttgagcagcg
     7021 cgaatcttat caatcagtgc actagcgtca cgaccgattc catctgcatt gatttccata
     7081 gcttgaacga ttccgtctgg atcaataata aatgttcctc gctgagctaa tccttgctct
     7141 tcatccaaaa catcaaaaat acgagaaatc tgatgagacg gatcaccgat catcacgtat
     7201 tcgattttac cgatagcatc tgatgtatca tgccatgctt tgtgagtaaa gtgggtatcg
     7261 gtagagcaag aaaagacttc aacacctaaa gatttcaatg tactgtattg ttcttgtaag
     7321 tcttctaatt cagtaggaca aacaaaagta aaatcagctg gatagaagca aagaatactc
     7381 cagttcccca taagatcttt ttcactgact tctaaaaatt ctccttgatg ataagcatgt
     7441 gcattaaatt cttcgatttt tttcccaatc aatgacatag taaaacctcc aataataatt
     7501 atttatttat aattattgta atttagttgg agttgcaaag tcaatggata ttttgttatt
     7561 taataaagat tactcaaaaa cgaaggttaa ttataaaaat agaagaggtc gcaagaaaat
     7621 gctactgctg gaaatactcg ggataacgtt gagtaacagt ttcctgttct ttcagcatca
     7681 tgtgtaagtg caatcccgtt tgaaatcgag cttcttctgg ttttttatca actaaagcct
     7741 tgagtaaatc ataatgttgt tccatgatcg tttcccatgg aagcccctga atggtcactc
     7801 gtaaccagcg gaaacgttct aaatgggtat tatggtcatc taaccagttc caaacttctt
     7861 ttcttcctgc aatttcgaaa caagcttcat gaaataaatt atctgcacgg aaaaaagcgc
     7921 gttcgtcatt tattttgact gctttttctt gttcttctaa aatcgtttct agcactattc
     7981 gtgtttgtgt gtccaatttg gaacaacatt caatcatgat ttgctcttcc aactgttctc
     8041 ggacaaagcg agcattttct gcagaaacaa gattaatttt ggaaacatac gtaccacttt
     8101 gaggtatcgt atacacgagt tcttgctgac gtagttgaat caatgcttcg cgaatgggag
     8161 tccggccgat agcaagatgt tcctccagtg ccttttcaga tattttcctt ccaggttct
//
LOCUS       DACDIH010000099         8163 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDIH010000099 DACDIH010000000
VERSION     DACDIH010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.099"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            228..1619
                     /locus_tag="IV281_002494"
     CDS             228..1619
                     /locus_tag="IV281_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8781456.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNERAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAEIDFSQEKIYR"
     gene            1678..2481
                     /locus_tag="IV281_002495"
     CDS             1678..2481
                     /locus_tag="IV281_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8781457.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGKRIVPEYNNAIRVFSKRRNPFNHMTVMFD
                     KKAILEVGNYQPLSGFEDYYLWARLLKAGYKGYNIQETLVYARTGKDMYARRGGMKYL
                     GSGLMGRYRIWKDGLGSVKDFIIVSCGHIFISLLPNQLRGKFYQTQLRK"
     gene            2514..3368
                     /locus_tag="IV281_002496"
     CDS             2514..3368
                     /locus_tag="IV281_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HAP8781458.1"
                     /translation="MKKIQQITLKMSQEIVDFCLKNQLLCYFCGGGAIGTVREQGFIP
                     WDDDLDFFMPREDYEKFYQLWVNSEKSKKYPIQKASKNYNDHNSFTTIRDADTTFIKT
                     YQRDLDIVHGITIDIFPLDVAPVSKFSRKIQKLWALVYALFCSQVVPEKHGGIMATGS
                     KILLSIFRSPRTRYKIWSFAEKQMTKYNNEPTGYVTELCVGPKYMGNVYPAKDFESAV
                     WVPFEDTEMPVPVGYDNYLSQVFGDYMQLPPEKDQVSHHEAVYIDPEHPYTMYKGKYY
                     LTKGAEKK"
     gene            3365..4066
                     /locus_tag="IV281_002497"
     CDS             3365..4066
                     /locus_tag="IV281_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAP8781459.1"
                     /translation="MITAIIIAGGVGKRMGQDIPKQFITIEGKPIIIYTLESFQHHPQ
                     VDRILVVCKSGWEETMWAYIKEFNISKVKWVITGGSKGQESINNGVQFLKDYSEDDDT
                     IIIHDGIRPLVDELVLSDVIVKCKEFGNGVTSLPYNEQIFIKKTEETTEQYVDRNTLR
                     RVSTPQAYQYGKLRAAYDRAVKEDIGMTDSSYTNTMMVDLGETLYFAAGSDKNIKLTT
                     TDDLELFKAYLKMKD"
     gene            4074..5141
                     /locus_tag="IV281_002498"
     CDS             4074..5141
                     /locus_tag="IV281_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP8781460.1"
                     /translation="MMTLIKQPMYQEDLAKVINHTPKIKQLHGKSVLMIGASGMIGSF
                     LIDTLMAANEQLGIKIKVYAMGRNRQKLEKRFASYLELDTFEIVEGDVTEPLPKEIQA
                     DYLIHGASNTHPKAYATDPIGTIMTNLAGTEQVLKHAVATQPVRVLFLSTVEIYGENR
                     GDIEKFTEDYCGYIDCNTLRAGYPEGKRVSESLCQAYIAKHDLDIVIPRLCRTFGPTM
                     LLSDTKASSQFILNAVEQKDIVLKSEGNQYFSYVYVGDAVSAILHLLLEGEKGEAYNV
                     SSENFDLRLKELAQQLADISGKQVIFELPDEIEQKGFSKASTAVLENKKIKETGWKPI
                     FPLNESLVHTVELVKGEKYNV"
     gene            5134..6099
                     /locus_tag="IV281_002499"
     CDS             5134..6099
                     /locus_tag="IV281_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8781461.1"
                     /translation="MCEISIIVPVYKVEKYLRKCVDSILAQTFTDFEVILVDDGSPDN
                     SGKICDEYAEKDNRVRVIHKENGGLSSARNAGIDVARGKYLGFVDSDDYIDEDMYEIL
                     YENLKIHDADISSVELIPFYGDRYKKANKEKKVIILNKKEAIKSVLEGTQFYAYAWNK
                     LYRKELFKDNRYLDGKTFEDAYIIIDLLFQTEKIVVSNEEKYFYLQRNDSIMGKSFSM
                     NNFDVIEAWQYNKEKILDAFPDLHDSYYRRLCWAYFYVLDKMVLSSSYQQIPRKKTVI
                     EFLKKNRNFILTYKGFTKSRKIAIIALSFRVDWYRKLVEQKNKKN"
     gene            6144..7118
                     /locus_tag="IV281_002500"
     CDS             6144..7118
                     /locus_tag="IV281_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8781462.1"
                     /translation="MCRISIIVPVYNVEKYLKKCVDSILGQTFTDFELLLIDDGSTDN
                     SGSICDELAKTDNRIKVIHKENGGLSDARNIGIEVAKGDFIGFIDSDDYIDEDMYAFL
                     YNNILKYDAELSMCGIYDVHKNKEIKKLTPFSQLVTKSEAIELVLDGKLVVANAVSKL
                     YKKELFENVRYPKGKIAEDAAVILKIINQCNKIHVDTSQKYYYYHRGDSITSKKFTKK
                     DFDIIDVWNENEKWIIKNYPQMYDKVHTRVCWAYFVVLDKLVLTEEKGLYQEKKQVRE
                     YLIDNFFFIIKNKRLTFQRKISMLLLTFGIAAYKIPVKYQYKKVKAVN"
     gene            7189..>8163
                     /locus_tag="IV281_002501"
     CDS             7189..>8163
                     /locus_tag="IV281_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAP8781463.1"
                     /translation="MFIYILRIVIQFVGRSFFIYFLGAKYLGLNGLFTNILSFLSLAE
                     LGIGSSIIYSLYKPLAQKDESKVKALMNLYKKTYEFIGIFIGIVGVLIIPLLPLFIKN
                     STDIPHMYRYYVLFLLNSVLSYFFTYKRSLAIADQKNYLVAINDFAFLFFMNIVQVIA
                     LYFYSNFTLFLIVQILFTLFSNINISRTVDKRYSYLKEKDVVSLDKQTKDEVKKNVIG
                     NMSSKIGGVIVMGTDNILISTFVNLTAVGIYSNYSLIVTSVQNLCKQITNSITASVGN
                     FAVSNKKKDSFELFKKHFFINHSLIFFSSILLLTLMNPFISLWVGAKYM"
BASE COUNT         2940 a         1102 c         1514 g         2607 t
ORIGIN      
        1 ttgtaacttg tgaataagga attagcagga gaatactagt tcagcataaa ggaagtgatg
       61 ggacttctga gacaactttc tgttatgcag aaatacacat ataaacagac aataaacgct
      121 tttccccgtt tcttttaatt tcctttacaa tttggttatt cactaggttt ttaaactttg
      181 ctatggtaag atgatagagg cattatttta tggttaggag tgtatatatg aataaaaatg
      241 gggagtggaa tgcggcacgc agaatattca ttattcttat ggatgtactt gtattaaatg
      301 tatcgatcta tcttagtttt ttgctgaaat tccagggaga agtaccagtg agaaacttag
      361 aaacctttca gcattcagca atttttattt ccatcatctt tattgcgttg aatattttgt
      421 taggagctta tgtattctat aaccgaatga tcagtgatat tgtcttcatt actgttattg
      481 gacaagtact aatagcattg gggatcatgg tagttacttt tgctggccga tggtttgcat
      541 ttccgcgtgg cgtcattttg atctcgttta tcctaggtac gatattactt agtgtttacc
      601 ggatagtcgt ttacaaactg tacttaagag tcagtcgtga taagaaagtc cttatcgtgg
      661 gtttggagaa agatgtcgca ccagctatcc aaaattttca atctaagaaa aacaataaac
      721 ataaggttga agcagtggca atcgataatt attacgaaaa tacaaaaaaa ttgatcgatg
      781 atatggatat cgtctatttg gcttctaaaa tcccagaaga tgaaaaaatc aacatttatc
      841 aattagtgac taaaaaagag aagaaattat ttttaaatac gacttttgag aatctgacaa
      901 tgataaatcc gaatattatg aattttgaag atgaaagcat cattgaagct tctgggttca
      961 gaatccaacc agaatacgaa ctattcaagc gtctctttga tatcatcgtt tcgcttattt
     1021 tactgatcct cgcctcacct tttatgctaa tcacagcgat tttggtaaaa acaacttcac
     1081 ctggaccaat catctataaa caagtacgta ttacgaaaaa tcaaaaggaa ttttcaatct
     1141 ataaattccg ttccatgagt gcgacagctg aagcaaaatc aggaccagtc ttggctaaaa
     1201 gtaacgatgc acgtgtgaca ccagtaggta aatttatccg tgctgtacgt tttgacgaat
     1261 tacctcaaat ctttaatgta ttgaaaggcg atatgtcgat tgtcggtcca aggccagaaa
     1321 gaccattttt cgttgatcag ttcaatgaag aaaatcctta ttattactta agacacaatg
     1381 aacgtgcggg aattaccgga tatgcacaag tatacggaaa atatgtatca gattataaca
     1441 gtaaactacg ttttgatttg ttgtatatca aaaaatattc attactatta gatttgaaga
     1501 ttttacttca aacaatcaaa atcttatttg ataagatgtc atctcaagga ctagatgaag
     1561 atgaaaatat tagttcatta gcagaaatcg acttttcaca agagaaaatt tatcgctaga
     1621 ttggaaaaat tttgagcaaa gaggtaggag agaacattgg aaaataaaaa tgtaagtatg
     1681 ctgctttctt tatatgataa agaaaagcca gagtatttaa aggaagcatt aactagtatt
     1741 tttgagcaaa cagtaatgcc aaaagaaatt gtattagttt atgacggccc tataaatgaa
     1801 aaacttcaag tagttgtcac ttatttccaa caaaaagatc ctcacttgtt tacagttgta
     1861 aaattagaaa aaaatcaagg attggggatt gctttagcaa ttggattaga acatacgaaa
     1921 aatcgcttag tagccagaat ggatacagat gatataatgg aaccccaacg attagaaaaa
     1981 caattagaga tcttccaaaa agatccagaa atagcgattg ttgggagtaa tattgaggaa
     2041 tttactggca cgcttagcaa tgttatcgga aaaagaattg tacctgaata taacaatgca
     2101 attcgtgtat tttctaaaag aagaaatccc tttaatcata tgaccgttat gtttgataaa
     2161 aaagctattt tggaagtggg aaattaccaa ccattaagtg ggtttgaaga ttattatctt
     2221 tgggcaagat tattaaaggc aggttataaa ggatataata ttcaagaaac attagtctat
     2281 gcgcgaactg gcaaagatat gtatgcaaga agaggcggaa tgaaatatct tggttctggt
     2341 cttatgggtc gttatcgtat ctggaaagac ggtttagggt cagtaaaaga ttttataatt
     2401 gtttcttgcg gacatatttt tattagctta ctaccaaatc aattaagagg aaaattttat
     2461 caaacgcaat taagaaaata ggaggttttt ctttgtatag cactgatcca aaaatgaaaa
     2521 aaatacaaca gattacgttg aaaatgtctc aagagattgt tgatttttgt ctaaaaaatc
     2581 aacttctttg ttatttctgc ggtggtggtg ccatcggtac tgtgagagaa caagggttta
     2641 ttccatggga tgatgattta gatttcttta tgcctagaga ggattatgaa aaattttatc
     2701 aactatgggt taattcagaa aaaagtaaaa aatatcccat acagaaggct tctaaaaatt
     2761 ataatgatca taattctttt acaactatcc gcgatgcaga tactacattt attaaaactt
     2821 atcaaagaga tttagatata gtccatggaa ttactattga tattttcccg ttagatgttg
     2881 cccctgtttc gaaatttagc cgtaaaattc aaaaactatg ggctttagta tatgctttat
     2941 tttgttctca agtcgtccct gaaaaacatg gtggaataat ggctacaggt agtaagatac
     3001 tcttatctat tttccgctca cctcgtacga gatataagat atggtctttt gctgaaaaac
     3061 aaatgacaaa atataataat gaaccaacgg gctatgtgac tgaactttgt gtcggaccta
     3121 aatacatggg aaatgtttat cctgcaaaag actttgaatc tgctgtgtgg gtgccattcg
     3181 aagatacaga gatgccagta ccagttggtt atgataatta tttaagtcaa gtttttggtg
     3241 actatatgca gcttccgcct gaaaaagatc aggtttctca tcatgaagca gtttatattg
     3301 atccagaaca tccatacaca atgtacaaag gaaaatatta tttaactaaa ggagcagaaa
     3361 aaaaatgatt acagctatta ttattgctgg tggtgtgggt aaacgaatgg gacaagatat
     3421 cccaaaacag ttcattacaa ttgaaggaaa acctattatt atctatactt tagaatcatt
     3481 tcaacaccat cctcaagttg accggatttt agtcgtttgc aaatctggtt gggaagaaac
     3541 gatgtgggcg tatatcaaag aattcaatat tagcaaagtc aaatgggtca ttacaggtgg
     3601 atcaaaggga caagagtcga tcaataatgg tgttcagttt ttgaaagact attctgaaga
     3661 tgatgatacg atcatcatcc atgatggaat ccgtccacta gtagacgaac tggtattgtc
     3721 agatgtaatc gttaaatgta aagaatttgg taacggtgta acctctctac cttacaacga
     3781 acaaattttt atcaagaaaa cagaagaaac gacggaacag tatgtcgatc gtaacacatt
     3841 gaggcgtgtc tcaactccac aagcttatca atacggaaaa ctacgggcag catatgaccg
     3901 ggcagtgaaa gaagacatcg gtatgactga ttcttcttat acgaatacca tgatggtaga
     3961 tttgggagaa actttatatt ttgctgctgg ttctgataaa aatatcaaat tgacaacaac
     4021 agatgatcta gaattgttca aagcatattt aaaaatgaaa gactaggtga aagatgatga
     4081 cgttgattaa acaaccaatg taccaagaag atttggcaaa agtaatcaat catacaccaa
     4141 aaataaaaca gctacatgga aaatccgttc tgatgattgg tgcttctgga atgatcggct
     4201 cttttttgat tgatacgttg atggctgcta atgaacaact aggcatcaaa atcaaagttt
     4261 atgcaatggg cagaaaccgt cagaagcttg aaaaaaggtt tgccagttat cttgaattag
     4321 atactttcga aattgttgaa ggtgacgtca cagaaccctt accaaaagaa attcaagctg
     4381 actatttgat acacggtgca agtaacacac atcctaaggc ttacgcaact gatccaatcg
     4441 gtacgatcat gactaatctt gcaggtacag agcaagtatt gaaacatgca gtagcaaccc
     4501 aaccggttag agtattattt ttatcaacag tagaaatcta tggcgaaaat cgaggcgata
     4561 tagaaaaatt cacagaagat tactgtggat acatcgattg taatacactg agagcaggct
     4621 accctgaagg aaaaagagtc agtgaatcat tatgccaagc ctacatcgca aaacacgacc
     4681 tcgacatcgt gattccaagg ctttgccgta cgtttggtcc gacgatgctt ttatctgata
     4741 cgaaagcatc ctcacaattt attttaaatg cagtcgaaca aaaagatatt gttttaaaaa
     4801 gtgaaggaaa ccaatatttt tcgtatgtat atgttggaga tgctgtttct gctattcttc
     4861 atttactgct tgaaggagaa aaaggagagg cgtacaacgt ttcttcagaa aactttgatt
     4921 tacgactaaa agaattagcc caacagctag ctgatatcag cggcaaacaa gtaatttttg
     4981 aattgccgga tgagattgaa caaaaaggtt tctcaaaagc tagcacagca gtacttgaaa
     5041 ataaaaaaat taaagagaca ggttggaaac caatttttcc tttgaacgaa tcattggttc
     5101 atacagtaga attagttaaa ggtgagaaat ataatgtgtg agattagtat tattgttcct
     5161 gtgtataaag ttgaaaaata tttaagaaaa tgtgtagatt cgattttagc ccaaacattt
     5221 actgattttg aagttatatt agtagatgat ggatctcctg acaatagcgg gaaaatctgt
     5281 gatgagtatg ctgaaaaaga taatcgagta cgtgtgatcc ataaagaaaa cggcggatta
     5341 agcagtgcta gaaatgcggg gattgatgtt gcgagaggca aatacttagg ctttgttgac
     5401 agtgatgatt atattgatga agatatgtat gaaattttgt atgagaattt gaaaattcat
     5461 gatgccgaca ttagctctgt tgaattgatt cctttttatg gtgatagata taagaaagct
     5521 aataaagaaa agaaagtaat tatattaaat aaaaaggaag caataaaatc tgttttagaa
     5581 ggaacgcaat tttatgcata cgcatggaat aaactttatc gtaaggaact ttttaaagat
     5641 aatcgttact tggatggaaa aacatttgag gatgcatata taataataga tctattattt
     5701 caaacagaaa aaattgttgt ttccaatgaa gagaaatatt tctatcttca aagaaatgat
     5761 agtattatgg gaaaaagttt ttctatgaat aatttcgatg ttatagaagc atggcaatat
     5821 aacaaagaga agattttaga tgcttttcca gatttgcatg attcctatta tcgccgacta
     5881 tgttgggcat atttttacgt gttggataaa atggttttat caagtagtta tcaacaaatt
     5941 ccacgtaaaa aaacagtaat tgaattttta aagaagaaca gaaattttat attaacttat
     6001 aagggattca ctaaatcgcg caaaatagct attattgctc tgagctttag agtagattgg
     6061 tataggaaat tggttgaaca aaaaaataaa aaaaattaga atgtttaaat tagtaaataa
     6121 aagaaaagga attgaaaact aaaatgtgca gaataagtat aatagttcca gtttataatg
     6181 ttgaaaaata tttaaaaaag tgtgtcgatt caattctagg ccaaacattt accgattttg
     6241 aattattatt aattgatgat ggttctacag ataattcagg aagtatatgc gatgaacttg
     6301 cgaaaacaga caaccgaata aaagtgatac ataaggaaaa tgggggtcta agtgatgcca
     6361 gaaatattgg tattgaggta gcaaagggag attttattgg gtttatagat agtgatgatt
     6421 acattgatga agatatgtat gcatttttat ataacaatat attgaaatat gatgcagagt
     6481 tatcaatgtg tggtatttat gacgtccata agaataaaga aattaaaaag ttaactcctt
     6541 tttctcaatt ggttactaaa tctgaagcaa ttgaattagt attagatggt aagctagttg
     6601 ttgctaatgc agtaagcaag ctatataaaa aagaattatt tgagaatgtg agatatccaa
     6661 aaggaaaaat tgctgaagac gcagcagtaa tactgaaaat tatcaatcag tgtaataaaa
     6721 tccatgttga tacttctcaa aaatattatt attatcatcg tggagatagt attactagta
     6781 aaaaatttac aaaaaaagat tttgatataa ttgatgtatg gaatgagaat gaaaaatgga
     6841 ttattaaaaa ttatcctcaa atgtacgata aagtacatac tagagtttgt tgggcttact
     6901 ttgttgtgtt agataaatta gttttaactg aagaaaaagg actgtatcaa gaaaaaaaac
     6961 aagtaagaga gtatttaatt gataatttct tttttattat aaaaaataaa cgattaactt
     7021 ttcaaagaaa aatatctatg ttattgctta cttttggaat tgctgcgtat aaaataccgg
     7081 ttaaatatca gtataagaaa gtaaaggctg ttaattaatg ctttagttag gatgaattag
     7141 atgaaaaaaa gcaggttaga gtattcttta ataaattctt ctatatcaat gtttatatat
     7201 attttaagga tagtaataca atttgtaggt agaagttttt ttatttattt cttaggagcg
     7261 aaatatttag gattaaatgg tttatttaca aatatactta gtttcttgtc gttggctgaa
     7321 ctaggcatag gctcttctat tatttattcc ctatacaaac cattagccca aaaggatgaa
     7381 agtaaagtaa aggctttaat gaatttgtat aagaagacat atgaatttat tggtattttt
     7441 atcggcatag taggagtatt gattattccg ctgttacctc tatttattaa aaatagcaca
     7501 gacataccac atatgtatcg atattatgta ttatttttat taaattcagt tcttagttat
     7561 tttttcacgt ataaaagatc acttgcaata gcagatcaaa aaaattatct ggttgcgatt
     7621 aatgattttg cctttttatt ctttatgaac attgtacaag taattgcttt gtacttttat
     7681 tctaatttta ctctatttct aatagtgcag attttgttta ctctattttc taatataaat
     7741 atttccagaa cagttgacaa gagatattca tatttaaaag agaaagatgt agtttctcta
     7801 gataagcaaa caaaagatga agtaaagaaa aatgtcatag gtaatatgtc ctcaaaaata
     7861 ggcggggtta ttgtaatggg gacagataat atattaattt ctacatttgt taatttaaca
     7921 gctgtaggaa tttattctaa ctattcattg attgtcacaa gtgtgcaaaa tttatgtaag
     7981 caaattacta attctatcac ggcaagtgta ggaaattttg ctgtatcaaa taaaaaaaag
     8041 gattcatttg agctatttaa aaaacatttt tttatcaacc actcactaat ctttttttct
     8101 tctatattgt tattgacttt aatgaatccc ttcattagct tgtgggtagg agccaaatac
     8161 atg
//
LOCUS       DACDIH010000100         8057 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDIH010000100 DACDIH010000000
VERSION     DACDIH010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.100"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            106..885
                     /locus_tag="IV281_002502"
     CDS             106..885
                     /locus_tag="IV281_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781464.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
     gene            926..1870
                     /locus_tag="IV281_002503"
     CDS             926..1870
                     /locus_tag="IV281_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8781465.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1886..2665
                     /locus_tag="IV281_002504"
     CDS             1886..2665
                     /locus_tag="IV281_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP8781466.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            2678..3376
                     /locus_tag="IV281_002505"
     CDS             2678..3376
                     /locus_tag="IV281_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP8781467.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3404..4168
                     /locus_tag="IV281_002506"
     CDS             3404..4168
                     /locus_tag="IV281_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP8781468.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYKQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEIKKKKFGLF"
     gene            4181..5998
                     /locus_tag="IV281_002507"
     CDS             4181..5998
                     /locus_tag="IV281_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8781469.1"
                     /translation="MFVLTRRRKIATLVVVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISTGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASDVSSILILLFINRQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIQVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAKGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNK"
     gene            6012..7235
                     /locus_tag="IV281_002508"
     CDS             6012..7235
                     /locus_tag="IV281_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HAP8781470.1"
                     /translation="MNKLEKRQIPFSPPDITDLEIKNVVEVLKSGWITTGPKTKKLEK
                     KITKWLGTSKVVCLNSQTAAAETGLRLLGIGENDEVIVPAYTYSASASVVCHIGANLV
                     LIDCQQDSLEMDYDALEAAITEKTKVIIPVDLGGIPCDYDRIFEIVEKKRNLFQPSNE
                     IQKAYGRIIVMADSAHAFGANWHDQMVGNVADLTTFSFHAVKNFTTAEGGALVWKEHD
                     KLDNEAMYHQCQLLSLHGQSKDALTKTQLGAWEYDIVGPWYKCNMTDVTASIGLAQFE
                     RYPNILKRRKNIIKRYDEAFQQLGIEVLMHYTDDYSSSGHLYITRVPNISSEQRNEII
                     IKMAEQGIACNVHYKPLPMFTAYKNMGFDINNYPNAYAHFVNEITLPLHTKLTDEEVE
                     YVINNYSKIVKDLVG"
     gene            7249..7983
                     /locus_tag="IV281_002509"
     CDS             7249..7983
                     /locus_tag="IV281_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006443581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8781471.1"
                     /translation="MRKWEDLPKEMQNDEIRYYYDILSKKKFSLVVKRVFDILVSATL
                     LLTLSWLFFILAIVIKIDSPGPVFYRQERITRYGKKFKIYKFRTMVINADKDGTLVTV
                     KNDDRITRVGKVIRKYRLDEISQLINVLVGDMSFVGTRPEVGKYVKSYSPLMLATLLL
                     PAGITSETSILYKDEDKLLHDKNNIDKTYIEKILPSKMYYNLKSISEFSCMNDLKIMC
                     KTIFVIFGKEYHADLEGEREFINQIK"
BASE COUNT         2838 a         1390 c         1673 g         2156 t
ORIGIN      
        1 tatgaatata ttagtttaat tatgctttta aaataaaaag cgctcattct gatgtgaatg
       61 aatcacatct ttatttatct atcatcaata tttaggagtg aatctatgga aaagccgttc
      121 gtcaagttat tggctactgt ggcggtaggc acaggcgcta ttgtgatttg cttgtttgga
      181 taccatctca ataatcagcg gcaacatcat caacggatca attacgcaga atctgccatc
      241 accaatcaaa aagacaccgt gacctcttta tcaaaagaag tagataagct gtacagtaca
      301 aaagaaaaaa ttttcctaaa tcctgaaata acagaagaaa cagtttcaaa tttgtcacat
      361 aaactatctt ctatcaaact ttcagcagat gattatgaca tcaaggaatc agaattacct
      421 aaagaagctg ctgcgatcca agaagaaaag aaagcagtct taacacagtt agaggatgca
      481 gaatcaaaac tgaagatcca aacggctgtc aataaattat ttactaaaaa cgtatcaaac
      541 tggcagcaag ctgtggatga tgtcatcatc aaagaaaagt tggcaagtgc agatgtcgcc
      601 catgtacgtg aaaatatgag cttttttaag gatagtgctt ggaaaacggt cgttatgcaa
      661 tatctaggat tcgctgatac ccaaattgca caagtcacgc aactagatca gctattcgat
      721 acgatgctga aagacggtca agtaacagct actgctactt acgatcaata tttgaccgca
      781 ctcagtctca tcgaacagat ccggaatgaa aaaataagag ccgcatacgc gtcaaaagca
      841 gaaacagtcg ctcagcaaat gggctatagc aatacgagct attaataagc gagaattcaa
      901 gcaaggctag caaacggagg aaactatggc aaaacgaaga agaagatcaa gagggaaaaa
      961 gatatttcta attattttag caattcttgt cgtattagta ttagcaatcg taggagtagg
     1021 agcaaaatta tatctagatc tctcacattc tattgaaaaa acttacgaat cagtggaacg
     1081 aacccaagga gaacgaacaa aaccagttga tctggacaaa caagaatctt tctctgttct
     1141 attgatgggg attgatacgg gagatttagg tcgggtcgat caaggaagat cagatacgat
     1201 gatggttgcc acagtcagtc ctgaagacaa acagacaacg attgtcagca ttcctcgtga
     1261 tacgtatgtc gacatcgttg gacatgacac aaaagacaag atcaaccacg cttacgcatt
     1321 cggcggagct gccatggcga tggataccgt acaaaaatat gtagacatcc caatcgatca
     1381 ctatgtttca atcaatatga aaggtctaaa agaactcgtt gatgcagttg gtggtatcga
     1441 agtcaataat gacctcacct tctcacaaga tggctatgat tttacgatcg gtaaaatcac
     1501 attgaacggg gatcaggcat tgtcttattc tcgtatgcgt tatgaagatc caaatggtga
     1561 ctacggcaga caagaacgtc aaagaaagat cgtagaaggt gtagcgaaaa aagtgttgag
     1621 tctgaacggt gtaagcaact atcaatccgt tttgaatgct ctagagtcga atatgaaaac
     1681 agacatgagc tttgatgaaa tgaaaaagat tgctttagat tatcgggatg catttggtac
     1741 gatcaagcaa gatcagctgc aaggagaagg cttcatgcaa gacaatatct cataccaaaa
     1801 agtcagcgat caagaacttg atcgtgtgaa aaaagaatta aaagatcaaa tgaatctaga
     1861 gaataaatag gaaagtggag aaaaaatgga agagacaatc agcttgaaag aaatttttga
     1921 aacattacgc aaacacgtga taacaatcct catctcaatg tttgctggct tggccatctc
     1981 aggcatcgtg acgtttttca tcttgacgcc aaaatacagc tcacaagccc aattgatcgt
     2041 gacactgccg caatcagata ctgcaaatgt caatgatgtc aatacgaacc tgcaaatgat
     2101 caatacgtat aaggatatga tcgtcagtga tctagtacta aatgaagtca aagatcgttt
     2161 agaaacagaa gacaatgtaa aaatgactgc tggacaaatc aaagatgcca tctccgtcaa
     2221 tcagtcacaa aattcacaaa tgttctcgat ccaagcaaca agcacaaatg cagtgacggc
     2281 tcaacaaatc gctaatacaa cgtcacaagt attccaagag aacgccaaag acgtgatgaa
     2341 tgtcgataaa atctcgatca tctcaggtgc agttgccaat acgacacctg tttcgccaaa
     2401 taataaattg aatctagtga ttggcttagt gttaggaatg atggtaggcg taggtctagc
     2461 tttcttattt gaattactcg accggacagt aaaagatgaa aaatatgtga cggatacatt
     2521 gggcttcccg atcttaggga ctgtaccaga aatgtcagcg aaagaattga atgctggtat
     2581 ccgtaaaaaa ggaccagctc cgacacaacc aaaagctaca aacgataaaa cagcgccatc
     2641 acgcagaagc cgctcaagag tctaggagga aagaaaaatg gcacgaacac agaaacaaaa
     2701 agcagcccaa actcatgcag tcagtttggt cacactcaca aacccctctt caccaatcgc
     2761 tgaacaatat cgtacgattc ggacgaatat ccagtttgcc tccgcagctg gtcagcaaat
     2821 taaaacgatt gttgtgactt catcaggacc tggagaaggg aaatcgacca ctgcagcaaa
     2881 cattgccgtt gtttttgcca aatctggtca acgggtgctg ctagtcgatg cagacttacg
     2941 taagcccgtc atctataaaa cgttcaagtt gaacaacgca agcggattaa gtaccgttct
     3001 tagttcatca ggaagtgtag cagatgagat ccaacgaaca ccagtcgaga atttgtcgat
     3061 tctgccaagt ggacctaaac caccaaatcc atcagaacta ctaagttcgc caagaatgga
     3121 tcagatttta gcagaagcaa gacaactatt tgatgtagtg atcttcgaca tgccgccagt
     3181 agttgccgta acagatgcac agatcatgtc ttccaaaacg gatggcacct tattggtcgt
     3241 acgtgaaaat atgtcacgga aagaatcatt gactaaagca aaagaattgc tggatatggt
     3301 acaagcgcga gtccttggtg ttgtatacaa tggggcagaa cacagcaaag atgctggcta
     3361 ttactacggg aattaagaaa taaaaagaga ggagacaaga gaaatgatcg atctacattg
     3421 ccatatcctg ccaggaatcg atgacggagc agaaaacata gatgccagta tcgccatggc
     3481 tgaaaaagcg attcaacaag ggatcacaca tattttatgc acaccccatc ataataatgg
     3541 aaaatacagc aatgaaaaat cacaagtcat ttctctagtc gcttcgttac aagcagaatt
     3601 agagaaaaga caattgcctt tgacgttatt ggaaggacaa gaagtacgta ttaccggaac
     3661 actgatcgaa gacatcgacc gagatgaaat tttatttaca gatttagatg atacatatct
     3721 tctaatcgaa tttccgacac tggaagtgcc gttatatgca gaacagctct tttttgagtt
     3781 gttacaacta ggaaagacac cagtgatcgt ccatccagaa cgcaacgcgc attttcgtaa
     3841 agacccgaat catatgctgc cattcctaga tatgggatgc ttggcccaac tcaccgcacc
     3901 aagttatgtc ggtgtatttg gaaaagacat ccagaaaaca gccaaaatga tggtcaaaca
     3961 taatcttgtg cagatggctg cttctgatgc ccatggggtc tctaagcgca ccttttactt
     4021 aaaagagtgc tataagcaga ttgccaagga ctttggtaag gaaaaagtag tacagatgaa
     4081 acaagtcgca aaggatctga tcaacgggga tgaaatcagt tatccagtct atgaagagat
     4141 aaaaaagaag aagtttggtt tattttaggg ggagtgtaag gtgtttgtat taacaagaag
     4201 aagaaaaatt gccacattgg ttgttgtgga cagtttgtta ttgatcgctg caaatattgc
     4261 agcagctaaa ttcatgaagc cgtttgtagc tataccaatg gaccttattt taatctctac
     4321 cggtttgtcg attggttttt atttatttta tggtagttta ttcaaagttt tcactcggat
     4381 caatcgctat acaaatttaa gagagatcat tgcaattttt ggttcactat ctgcaagtga
     4441 cgtctcaagt atcctcatac tgctgttcat taatcggcaa tacagtttac ggttagttat
     4501 ctttgcttat ctgctttcgc tcttattgat catcggtagc cgattgatct ggcgtatcta
     4561 catcgaaaca aaaaatatga gatatgtatc agcggattct gctaaaaata cattgatcgt
     4621 tggtgctggc gaaggcgggc gtattttata caacagcttt ctgggatcca aaacggctca
     4681 agatatccaa gttgtcggat ttgtagatga tgatccgaat aaaagaaata cgtatctttc
     4741 aggaaaaaaa gtattaggtg cattaaaaga tattccggaa ttgattgaaa aatatgacat
     4801 tcaaatggta acgatcgcta tcccttcctt atcaagaaaa aaattacgaa gaatcttcga
     4861 gttagtcgaa tccgctcacg tgaaagtcaa cacgatgcct tctatagaag aacttgcttc
     4921 aggaaaaatt agcgtatcaa aattaaaaac aattgacgta gtggatttac tgggaagaga
     4981 tgaggtaaag ctggatattg aatccatcaa agatcagatc acagacaaag tcattcttgt
     5041 caccggcgca ggaggatcga tcggttcaga aatctgtcgg caaatcattc aattcaatcc
     5101 tgcaaagcta ttgcttttag gacatgggga gaactcgatc tacttgattg accgtgagtt
     5161 acgcacgcat catcaaaatt gtcctacaga aatcgtaccg atcatcgcag atatccaaga
     5221 tcgcgagaaa atcaatgaga tcatggaaca atatcatcca gatatcgtct accatgcagc
     5281 tgctcataaa catgtaccac tgatggaata taatccaaaa gaagcagtca aaaacaatat
     5341 tttcggtaca aaaaatgtag cagaagcagc taaagcagca aaagtgaaaa acttcgtcat
     5401 ggtatcgaca gataaagcta acaatcctcc aaatgtcatg ggttcaacaa aacgaatcgc
     5461 tgaaatgatc gtgactggat tgaatgaaga aggctgtacg aaattctcag ctgtcagatt
     5521 tgggaatgtc cttggttctc gcggtagtgt gattccagta ttcagagaac aaattgctaa
     5581 aggtggtcca atcactgtaa cagactttcg tatgacccga tacttcatga cgattcctga
     5641 agctagtcgt ttagttattc aatctggcgc attagcaaaa ggtggcgaaa tttttattct
     5701 agatatgagt gaaccagtga agattgtaga cctagccaaa aacatgatcc gattgagcgg
     5761 gtatagcgaa gacgaaatcg aaatcattga aacaggtatc cgtccagggg aaaaactata
     5821 tgaagaacta ctgttggata aggaaagaaa tgatgaagca gtttatgaga aaatctttgt
     5881 aggaaacatc aaaggttact caatacaaaa agtgatggat tttgtcaaga gcctgccgca
     5941 agacgatgaa caattggcta aagatatcgt cacttttgca aatgcttcga ataaataggg
     6001 gggaaatata aatgaataaa ttagaaaaac gtcagatacc attttcaccg ccagatatta
     6061 ctgatttaga aataaaaaac gttgtagaag tattaaaatc tggatggata actacgggac
     6121 caaagacaaa aaaattagaa aaaaaaatta caaagtggtt aggaacttct aaagtagttt
     6181 gtttaaattc acaaactgct gctgcagaaa cagggttacg tttgctggga ataggagaaa
     6241 atgatgaggt tatcgtccca gcttatactt attctgccag tgcatcggtt gtatgccaca
     6301 taggcgcgaa cctagtatta atagactgtc aacaagattc tttggagatg gattacgatg
     6361 cattagaagc agcaattacg gagaagacaa aagtgattat tcctgttgat cttggtggta
     6421 taccttgtga ctacgaccgt atttttgaga ttgtggagaa aaaaagaaat ttatttcaac
     6481 ctagtaatga gattcagaaa gcttatggtc gtataattgt tatggcagat tctgctcatg
     6541 catttggagc taattggcat gatcagatgg ttggtaatgt tgctgacttg acaacatttt
     6601 catttcatgc tgttaagaat tttacaactg cagaaggtgg agctttagta tggaaagagc
     6661 acgacaaact agataatgag gcaatgtatc atcagtgcca gctgctttct ttgcatggac
     6721 agtcaaaaga tgcacttact aaaacacagt tgggtgcatg ggaatatgat attgtgggtc
     6781 catggtataa atgtaatatg actgatgtta ctgcatcaat tgggttggca caatttgaac
     6841 gctatcctaa tattctgaaa agaagaaaga atataattaa gaggtatgat gaagcctttc
     6901 aacaactagg aattgaagtt cttatgcatt atacggatga ttatagttct tcaggccatt
     6961 tatatattac tagagtacct aacattagtt ctgagcaaag aaatgaaata attataaaaa
     7021 tggcagaaca agggattgct tgcaacgttc actataaacc acttccaatg tttacagcat
     7081 ataaaaatat ggggtttgat attaataact atcctaatgc ctatgctcat tttgtaaatg
     7141 agattacatt accattgcac acgaaactta cagatgaaga agtagaatac gtaattaata
     7201 actatagtaa gattgtaaag gatttggttg gatgatagga gaggcaaaat gagaaagtgg
     7261 gaagatttgc caaaagaaat gcagaatgat gaaatacggt attattatga tatcctcagt
     7321 aaaaaaaaat tcagcttagt ggtaaaaaga gtatttgata ttttagtgtc tgcaactttg
     7381 ttactgacac tttcatggtt attttttatt cttgcaatag tgataaaaat tgattctcct
     7441 ggacctgtat tttatagaca agaacgtatt actcgttatg gaaaaaaatt taaaatttat
     7501 aagtttcgta ctatggtaat aaatgcagat aaagatggca ctcttgttac tgtgaaaaat
     7561 gatgatcgta ttacaagagt aggaaaggta atccgtaaat atagattaga tgaaattagt
     7621 cagctaatta acgttttggt cggagatatg tcttttgtag gaaccagacc agaagttgga
     7681 aaatatgtaa agtcgtattc accattaatg ttagccacat tattattacc agccgggatt
     7741 acatcagaaa ctagtatttt atacaaagat gaagataaat tgttgcatga taaaaataac
     7801 atcgacaaaa cttatataga aaaaattctt cccagtaaaa tgtattacaa tttgaaaagt
     7861 atcagtgaat ttagttgtat gaatgatttg aaaataatgt gtaaaactat ttttgttata
     7921 tttggtaaag aatatcacgc cgacttagaa ggtgaacgag agtttattaa tcagataaaa
     7981 tgaatggagg ataagagttt tggtgattga agagataaaa agttcggata aagagttaat
     8041 taattcgatt gtcgata
//
LOCUS       DACDIH010000101         7630 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.101, whole genome shotgun sequence.
ACCESSION   DACDIH010000101 DACDIH010000000
VERSION     DACDIH010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.101"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            246..1547
                     /locus_tag="IV281_002510"
     CDS             246..1547
                     /locus_tag="IV281_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8781472.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1574..1834
                     /locus_tag="IV281_002511"
     CDS             1574..1834
                     /locus_tag="IV281_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8781473.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVTHLNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2833..4068)
                     /locus_tag="IV281_002512"
     CDS             complement(2833..4068)
                     /locus_tag="IV281_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP8781474.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            4636..5463
                     /locus_tag="IV281_002513"
     CDS             4636..5463
                     /locus_tag="IV281_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8781475.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            complement(5736..6506)
                     /locus_tag="IV281_002514"
     CDS             complement(5736..6506)
                     /locus_tag="IV281_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8781476.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            6724..>7630
                     /gene="ulaG"
                     /locus_tag="IV281_002515"
     CDS             6724..>7630
                     /gene="ulaG"
                     /locus_tag="IV281_002515"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP8781477.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKF"
BASE COUNT         2375 a         1182 c         1514 g         2559 t
ORIGIN      
        1 acaaaatttt tttgaaatga tatgtttcaa tcactagatt taatcatttt gatttttgtt
       61 aattatcgaa tttttttgtt gctcctatga tttagaaaac ttattttcag gattcttaaa
      121 aataaatttg attatttaag agaatatatt gaaataaaat ttcttaatta tataattagt
      181 tatgaacatt ctttgcgttg tcatgcaatg aatggttcat attttattga aaaggagtgg
      241 ttttagtgga aaagctgatg aaatgggtcg aagagaaact tgtcccacca atggcaaaaa
      301 ttggtacaca gcgtcattta ttagcaattc gtaatggggt tgtttcaaca ttgtcattga
      361 ttttgattgg gacatttttt atggtcttta tcaatttacc gtttcctgga tggaatgaat
      421 ttattgcccc ttattcggca acgattgttt taccctttag gatgacgatg ggattgatgg
      481 ctatttatgc aacttttgtt atgggttcgg atctagctaa atcatatgga ttagatagtg
      541 tgacaggtgg tatattatct ttaggaacgt tctttatgtt acaagttcct gtaaacgttt
      601 taactcctga agaggcacct cttggctggg tcttacctat gagttctttg ggagcatctg
      661 ggatgtttgc ggggatctta tctatgattt ttgcagtgga agtatatcgt ttctttaaaa
      721 atcgaaatat tactatcaaa atgccagaac aagtgccacc tgctgttgcc cgtagttttg
      781 aggcattgat tccaggtgca gtcattttaa caattacttg gctgattcga agcgtcgcgg
      841 gctttgatgt aaacgctgcc ttattatcgt tattcgagcc tttaaccaat attttaggga
      901 ataatttgct tggtgtttta ctaccaatgt ttttgatcca tttgttgtgg tcatttggta
      961 tccatggcat gagtattgta ggggctgtcg ttcgtccaat gtggctgatc atgttggatg
     1021 agaatgcaaa ggcattggca gatggtacag ctgccaccca attaccctat atcgctcctg
     1081 aacagtttta tcaatggacg gtaacaattg gtggtgcagg agcaacgatc gtggtatctg
     1141 ttttgtttat attcttctgt aaatcaaaat tcttgaaaga ggttggtcgt ttttctatta
     1201 ttcctggtat cttcaatatc aatgagccca taatttttgg agctcctatg atgttgaatc
     1261 cttacatgtt catcccgttc aacttagttc ctttagtttt gactattatc agttattttg
     1321 cagtcaaatt agaaatggtc aatagattta ctgttttgcc aacatggaca ttaccagctc
     1381 caatcggcgg ttatctttca gcaggaaatg actggcgtgt tgttatcttg atcatgatca
     1441 acacattgat agcatttatt atctattatc catttgtaaa agcttatgat aaaaagatgc
     1501 tgtcagatga acaaagtcgt ggagaaaacg aagtccagac tgtctaagtt tggtttttag
     1561 aaggtgggaa acaatgtatt ggctgatttt attttttgtt tttatctttt tattaacggc
     1621 tagtcacctt atattgaata tgctagctac atatcatatt caaatcaatc gatggatttg
     1681 ggcactggca tcatttttga tcgtgattct tccaaagatc attgtaacgc atttgaatgt
     1741 tttgttttca tgggggacat atgtactttg tggaattttt gctatcaatt ttatgatcga
     1801 gcagcataga tggtttgtca catcgaagct ttaaatgttc taaaagtggt tgtaacagaa
     1861 gtggttagct tcaagaaata agaagaaatt tacgaaaatt gcttcttaaa tttttgtgaa
     1921 atgttggctt atttttcgtt tggttgaact tattaccgca gatgcaagct tttgaccacc
     1981 gtttattcgg aatatgggac tgtgacaagg cttttgtcac agcccttttt tacataaaga
     2041 ttattattgt tttaactgtt ttggcagaaa gaaggaggaa aaaatggaaa ttttatcagc
     2101 aatggattta gaattagtag atctactacc aaagtgatca agtcaaatat agatttggga
     2161 gcttacaacc atatagtagg ggctgcagta tggtgtatga atggtaatat gtatgatgtg
     2221 agtgtttatt caaatcatga aacgtgtata gaaataattg tgctgggaat agcaatcagt
     2281 catggtgaac gtgactttta gtgtatcgtt gctgacgaca tagaacattc tgacatgatc
     2341 tactcaatat gtggggattg ctggcagttt atataggagt atgcatcgga ttgcgagatc
     2401 attgtctcaa cagaagaggg aggttgatta tcaaagataa agtggtaaat cctacatttg
     2461 atttagtaat tgttgagggg actagcggca caaatctcta caaagcgatg cttctagatc
     2521 tatatgacac attttaagaa aatgtatttg cctaattatt atggtctttc ttttgaagaa
     2581 acgttgagat gatccaacca gagaaaggtg tcaaagaatt tgactaagaa taaatggcag
     2641 acgctaccta tgctcatttg gatagcgtca gatcttttat tttaaggatg attttgtgtt
     2701 ttttagacac cgtaatttaa gtaactctaa cttttcaatc cactatttac tttttttgta
     2761 ttttcactgt ctgggtctcg aatagcgaaa ttactgatga gtctctatta aaaaagcttt
     2821 ttctacttga aactatattg ggtggttgct caggaaggtt tcgatttctt gaattgcttg
     2881 ttgtggacca tttattggaa aaacggcttg cagcgtgcgt gcctgctgac gaatcttggg
     2941 tgaagaaact tcgcgcagag caatctgcag ttttctttca gtcactggtt ttgtgagggc
     3001 gattgcgttt ccgtatttta cacaatagtc aatattacag ctttgctcaa attgcatccc
     3061 cacaccgata aacggtttgc cggattcaca ggcagtttgg acggtccctt ccccgccgtg
     3121 gataaaagaa aaatcaatga attcatgcaa aagatgacta gacagatatt ctgttattaa
     3181 aagatcttct gagtcagatt gaatgtcatc aagcagatag tcaataggtg caataaccgc
     3241 tagatccggt tgtttttgta agaagaacaa aattttgcgt aacatacgcg gattgcctga
     3301 actacccatt gcgaaataga taatacgttt tctttcttgt tgctttcgct gtataaaagt
     3361 tagcacttct tcaggcaagg aagtactgag cttagcaaaa attggaccaa cgattcgata
     3421 attttccggc agctgttctt cttccaaaaa tagtttaggc gttgtgatga gatttagatc
     3481 agcatccatc atttgtaaac ttgtttttgg taaaggaagc atttcttttt gtgcagcgtg
     3541 tttgaaggca ttggggatcc atcgggtatt tactatacgc ttttttgtcc agtgccaaag
     3601 gggattgctt aaagggccga agaaacgtaa ctcgtggggc atcagcgcat gctctgattg
     3661 aaaaaaaggt tcactcaaag catagggttt aacatatact aaaggaatct ttttattgcg
     3721 ggccgataaa aaaattgtca cattcgaacc tgtcacaatc agtcgaggct caaacttagc
     3781 aatcagactc tgttcactag tgatacgaga ggcaaccatt tctttggtaa aagggttttt
     3841 aagtgtacgc aactggtcaa attttatgat ttgttcttct tgttttttcg taaggatcgg
     3901 cgctagtaaa tgatactcaa agccactttc tgtgatcaaa ggagcaaaag ttttagaaaa
     3961 gccaaagaat acacagtaat aacggtcttt catagctttg gcaacctcaa tcattcgtgt
     4021 tgtctcagct aaattgaata ctgcaggagc aaaaagaatt gtcttcatga aatcctcctt
     4081 attggaatga gtctatccct tcgtttctta ctagtatact aaaaaacaca gcgttcgagt
     4141 agcctatgta tattttgaat attaaactgt ctctcgctgt tttcacaact tctaaattta
     4201 aattttgtaa tcaagttgaa actccaattt tcaaaaaaac acgcaacaat tttttgatcg
     4261 atttatatgt tgtgccagat aaatacgaga agttgtgtca ttaaaaataa acatgagaac
     4321 ataaaaaaat aaaataatta tattaattga taggtgtgct gtcgtcatta tacacacgta
     4381 agaaagaaat agttatgttt aaaaatattc ttagactttc tcgccatgac aattttcact
     4441 atgtgttata actgtaaacg ataattattc agataacaca aaaagaaatc aaggagcaga
     4501 tacgccagtt gtttaatttg aatattattt taaaattaaa atttttattc tgttctaaac
     4561 atttttggtt gtttagaact tttttttgtg tatgatataa aggagaaata attttttaaa
     4621 gggaaggaaa ttgttttgat taaagtagat ggagcattac taaaagaata ccgaaagaaa
     4681 aaggggttaa cacaagtaca attagcagaa ggcatttgtg aacaagcaac tgtcagcaat
     4741 atcgaaaaca agaatatgtc gccaggcatc aatatcttaa gtgcgctttg caaaaagctg
     4801 aatgtcgaga tctccgatat attagtagaa acagaagaag acaaaatcag gaaaaaaata
     4861 aatgagattc aatcattgaa ttctgtatca aaataccaag aatcttataa aattttacaa
     4921 acgatcgatc aggaaaaagt aagtaattct gatcttttaa atcaaatttt attttatcaa
     4981 ggactgcttg atcatcttta tgcgaaaaaa ccagatgcag cattatttta ttttaatcaa
     5041 gtgttggaac aaacgaccga aacagatatc catcacttac aagcagctgc aaacgtggca
     5101 atgatctact tgtcaaaagg agaattagat tttgcgaaag tttatgtgga gcgtacactt
     5161 aaaatattga gtgagactga ttttgataat ctgatggtct gcattgtata ttatgatatc
     5221 gccacctatt accgtaaaat cgaggattat gataaagcaa tccaattatg tgagaagggt
     5281 attgagtata ataagaaaca taaatcgact tatgcattgg aatacttgct ttatgaaatt
     5341 gcttcttgcc ataagcaatt aggagaagat gattatttag aaagatatat ggacgcaaaa
     5401 aaaatagcgc gtttcaacgg aaatgattac gcggtaaagg tgattgaaaa cgacctgaaa
     5461 taaagtggtt gtgaaatcag tgttctgatt tgaataccgt ttattcgaaa aaaggaggtg
     5521 tgacttttgt catacctcct tttttcgata ttgtttgatt cctatttagg caacatataa
     5581 cggttataaa agaagcaata gattatgatg gtatcgattg gatggataca tcttgggatt
     5641 cctttatcaa tttgagcgta gcgtgttttt catttatcga taaatttgtt attcattttt
     5701 ttgataatcc tatttacata tttacgtcct ggttcttaaa atgaatctac ttgaatggtt
     5761 tttatccccg tatttctgat ctcctctaat actttttcag aacaaaattt atcagtgatc
     5821 aaataatcaa agactgaaat atcagcaatc ttgaactttg atgtcagtcc gatttttcga
     5881 tgatcagcga ctaaaatctt acatttgatt gtcttgttga ccatcatttc gttgatcgtt
     5941 gattcattta atatctttgt tgtgacccca tgtactacat cgactccgct acagccaatg
     6001 atacaaatgt ctgcattgac tgaagaaatc gtatttaatg cgatgtcgcc cactagtgct
     6061 tcttttggaa agcgcatctc tccaccagtt aaaatataac tacaattatc tttgtgcgga
     6121 gagtaattga ttttcacgtt gtttgtaatg actgtcaaat ggttagattg aagaaaatcc
     6181 agtgttttca acgcagtcga gctagaattg ataaacagcg tatcataatc gttaatgtat
     6241 ttactagctt ctctggcaat cgcttgttta atcctttcag gaccagattg atcaaaatca
     6301 gtctggtttt tgtaattata agaacaataa ccgtaattgc gtatgatctc gtttttttct
     6361 tctaataaat ttaaatctct tcgtatcgta ctaatagata catcaaggga ttcagctaat
     6421 tcgaaagttg atagttgttc agtctgctga agcagttcca aaatttcttt ttgacgtttt
     6481 tcaatattga caagtgagtt tttcatgact tttgcctcct gagggacatc ttgatccatg
     6541 gatttatttg actagttgtt catttaaaca ttcatatctg tctttattat actgaaacaa
     6601 tcattgatta ctgaaagaaa atcatataaa aaacaaacgg tcatataaaa gggttttcat
     6661 ggatgctgat acacattaat atttacttgt aagttggaac aacgatatgg ggggagcaag
     6721 caaatgagta aaactgtcaa tgaagtaaca aaagaaaagt ggttagtaga aacatttcct
     6781 gagtggggca catacttgaa tgaagaaatc gaaacagaag tagtggatcc tggaactttt
     6841 gctatgtggt ggctgggctg tacgggaatt tggctgaaaa gtcatgaagg aacgaatatt
     6901 ttatgtgatc tatggtgtgg gacgggaaaa cgtagtcatg ggaatggcat gatgaaaaaa
     6961 ggccatcaga tgatgcgtat gagtggcgtt caaaatatgc agcctaattt acgtactcag
     7021 cctttcgtaa tcgatccttt tgcgataaaa aatgtagacg ctttaatcgt tacacatatc
     7081 cattccgatc atctagatat ccatacagcc gcagcagttc atcaaaattg tcctgaagca
     7141 acattcattg ggccaaaaga agtggttcgc acgtggcaaa aatggggaat tcctgaagaa
     7201 aaaacgatta cagtaaagcc aggtgatgca gtcaaaatca aagacatcaa tgtagtagct
     7261 ttagaagcgt ttgatcggac agcgttggtc acttgtgaag atccagaaga ggtattaaaa
     7321 ggaaaaatgc ctcaggagat ggatcaaatt gcagtaaact atttattcga aacatcagga
     7381 gggaatcttt atcatgcagg agattcacat tactccaatt tatttgccaa acatggtaat
     7441 gaacacaaaa tcgatgtctg tttaggggcc tatggtgaaa acccacgagg gataacagac
     7501 aaagttactt ctgttgatat gctgcgcatg gcggagtcat tgaatgcaaa agtagtgatt
     7561 cctgtccact acgatatttg gtctaatttt atggctgatc caaaagagat cactgaaatt
     7621 tggaaattca
//
LOCUS       DACDIH010000102         7616 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDIH010000102 DACDIH010000000
VERSION     DACDIH010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.102"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(16..708)
                     /locus_tag="IV281_002516"
     CDS             complement(16..708)
                     /locus_tag="IV281_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8781478.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            793..969
                     /locus_tag="IV281_002517"
                     /pseudo
     CDS             793..969
                     /locus_tag="IV281_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1145..1714)
                     /locus_tag="IV281_002518"
     CDS             complement(1145..1714)
                     /locus_tag="IV281_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8781479.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1788..2111)
                     /locus_tag="IV281_002519"
     CDS             complement(1788..2111)
                     /locus_tag="IV281_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781480.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2265..3077)
                     /locus_tag="IV281_002520"
     CDS             complement(2265..3077)
                     /locus_tag="IV281_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781481.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3431..4279
                     /locus_tag="IV281_002521"
     CDS             3431..4279
                     /locus_tag="IV281_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP8781482.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4424..5365
                     /locus_tag="IV281_002522"
     CDS             4424..5365
                     /locus_tag="IV281_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP8781483.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5355..7526
                     /locus_tag="IV281_002523"
                     /pseudo
     CDS             5355..7526
                     /locus_tag="IV281_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2270 a         1508 c         1371 g         2467 t
ORIGIN      
        1 agaggggggg attttttatc gttgtggaat atttaaatac caacgcttgt catgaaaatc
       61 ttgtgctcca cccttagtat ttccttctgg atcgtttgtc gcacgcatca tgacgtatac
      121 tttcttatta gggaaatcac gcatattgaa agatacatga tagccaacat ttcctaaagt
      181 attataagct tgatttacgt ctggtctata aatcccatca gctcttactc tagctagttc
      241 ttttccagta ttgtagtcca taatgaaaat atactcgtat ttataattag caatgtgcca
      301 tccagctaca tgcaagtttg cgttttcgat ttctccgaac tgatcaatgt gggcgtaatt
      361 tgttccatct gtcagcgtag aatttgcagc acctgctcga gttggatcaa ttactggttt
      421 atcctccgaa gtagttggat tatcatccgt aaaaccatga gccaaatcat aggctagttt
      481 ttctttactt acacctattt cagaaagata accgtaagga tctgtatgat cgccccaaat
      541 attttgtgtt acccataaat gcgatttgat tcctggttgg ttataaggag tgtctaatgt
      601 taatggaata ccatatttca ttgctgaatc tcttgccaat tcaacgtata ctttatagtt
      661 cttttcaaaa gtttctttat catgtgtgtg ttgtaactcg atttgcacag gacttttggc
      721 tcacgaggtt gtcctactct acatcttgct gatagatatg ctgaaaggaa aacaccaaca
      781 ttatgttttg ctttttcagc tagaacgaga caagcaggaa tacctttaga agatatcaaa
      841 ggttttttag gtcataaaga tgtttccact acacaagtct atgcccatat ttctcccgaa
      901 gtgaaaaaaa gatcaatgaa tcaactagaa aactatatag aagagcaaat aaaaaagcac
      961 tcaaattgag tgctttttta agctcactat cactaaaact atcactaatt ttatttataa
     1021 gcgaacatgt ttctatcact gattttttaa atctagcgaa aaacccttat cttaaaggcg
     1081 ttcgttcaat tctttggcaa ggtcttcaaa gcctggcttg cctagaagtg cgaacatggt
     1141 tttcttatag ctcatgtaga aaaattttct tgatgatgat tttatccatc aattgagatt
     1201 gactgttttg ctctcctatg atttcagcta tgattgtccg attttgcggt tttcttctta
     1261 actggtagtt gattttccca gtttccatca actcgctaag catatccacg actccgccaa
     1321 atccggtctt tttcgtaata aaacctagtg ctaccattcg ttcatctaga ttttttgctg
     1381 taatcgtcac accagtaatg agcgagtgct gtaatgtttg ggccagctct tccatccctt
     1441 cagcattgga tacgacggaa gctgttttgt taataggttc ttcgtttatt tcttttggtt
     1501 ctttcctctc cactttcact gtttgacgaa ggacatctag ttcttgcaag agcgtttcgt
     1561 attgttcttg ggtcagcatc acgccagcta ctttttcacg attaaatata tatacgccag
     1621 ttcccgcgtc tcgcgcttga gcaaatacat ccatcggcga tcgtttcact tctgtgatcg
     1681 aagatgtggg aacttctaat ttctttaatt ccaatggatt tcctccttta ctaatcgctt
     1741 tttattatag cacagactga cttgttttga tcttttttcc aaaggtttta tcggtttttg
     1801 cctaaccagt aattgatatc ggtaagaagt ctttcgatac caaatgaagg atggtacaga
     1861 tacgtttttt gatcttcgtt atggtctagt ggaaaaaaat gatcgatgat cactagatct
     1921 gcataccgag gcggcttatt tcgagtacat acatgtatag agatccgttt tcgatgtctt
     1981 agcgttttgt tcagtacccc agcaagtctt cttgcttctt ggtgttcata gcgaaaaaaa
     2041 aagacgattt tcacttcagg agggtggaca tctaaatacc ctcgttcctg gagtgttttc
     2101 agtaaaagca tcccccactc tactgcgggg tgtgcaagtg cttgttggat cgctagccga
     2161 aattgatata gtttatcttt ggaattggga aatttttcta atgtttcctg ataataacga
     2221 acttcttcta tcagccggtc atggatacca attacgtaac gaagtcattc ttggtaaagt
     2281 acttttgtca gctttgattt gctttcgctc tcaaattcgc tattcagcca aaacagcgaa
     2341 tcaagctttt cagtcacaat cgtcaatttc cgatagtctt gtgtctcttc tatccgtttc
     2401 caccgctttt cagtaatctg gttacagctc aagcgcaaaa ttgctagata atttttttct
     2461 tttattggca aaatctgctc ctctctaaat agcaattcag gaaacaattc aaagaatagt
     2521 gttcgactgt ctcctaaaat ctcgatttct gccggatcct ttctttgctt caataattgg
     2581 atgaacatct cagaaaagga ctgctctcca tcatctgcaa aaaaatgcgg aaaatctttc
     2641 aaaaagcaaa acagcagata tcttactttt ctttctgaac caaagatctg tgctttttta
     2701 taagaaaaag aaaggtggta ctcgccaaac cactttctca atcgctcttg cgtagaacga
     2761 tcgtacttct gctcgtaagg ttcattcaat agcagatttt tcagtaaaca aaacgggggt
     2821 gattggatca gaaaaacaaa caagagataa ggttcgctat atccttgatc ataaactaac
     2881 tgaacggttt tcttatgaag aatcagctga atgggacagt cgctttccgc caaacgtttc
     2941 agatgccgat ccactgtttt aacatcgatt ttcagttctt cagctaaatc agcttttcgc
     3001 cagatcaaat ctttcattag attgattagt gaaaataaat tctgtgcatt ttttgataat
     3061 agtttttgcc atctcatcga tcatttctca cctgatttct aaacatattg tttgatcaaa
     3121 atgggttcac tcctaaaaag ataaaaaact ccctttctat ttatttaaat acgcaaatct
     3181 tcctcattat tttttatttt actcaattta atcgtattct catttcaaaa cgaatgtact
     3241 ttaaaaaaac ataataagat cactttgctt gtaggatgta ttcccttcca ttacaatgaa
     3301 ataaatagcg attttatgag aaggtgatgt gaaaatggaa gaaaaaatag aagaatccaa
     3361 acaaaaaatc gatcagttga tataagaatt agaaaaagcg gtcatcggca aacgttcaac
     3421 tatccaattg atgatcgtgg ccctattagc aggtggtcat gtcttattcg aagatattcc
     3481 cggtgtaggt aaaacgttac tggtcaaagc actagcaaaa gctgtacagg gaaccttcag
     3541 cagagtccaa tgtactcctg atctgctgcc tagtgatatt cttggttttt ccgtatacaa
     3601 ttcacaatca aaagagtttg aatttcgtcc gggaccgatc tttaccacga tcttgttagc
     3661 agatgagatc aatcgtacga ctcctcgtac tcagtcagca cttttagaag caatggccga
     3721 gaatcatgca acgatcgaca acaatacata ccctctagac aatcattttt ttgtcttagc
     3781 tacgcaaaat ccaatcgagt acgaaggaac atatcctttg ccggaagcac agctcgatcg
     3841 ttttttattc cgactacaaa tcggttatcc ttcttttgat gacgaacttt tgctactttt
     3901 ggataaattc gaaaaagtgt tggataactt aaatgaagta ttgaacagtt atgaaataga
     3961 agaaataaaa acaaatgtgg cgaatgtctt tgtcagtccc gaagtggcat cttatgcctt
     4021 gcagttagtg tcagcaacaa gaagtcatgc tgccatccag cttggcatca gtccacgagg
     4081 aagcctttcc tttattcagg cagcaaaagc ttatgcttta atccatggga gaaattacgt
     4141 cacgcccaaa gatttgcagg atttagttcc ttacgtgttt tcccatcgcc tgatttttta
     4201 cgatcgttca ttgaaggaag acgaaaagaa acaaatcatt catactcttg ttgcacaaat
     4261 tcctattcct gtgaggtaga ttgaggtaga caatggaaaa aatcaaagat tatttcatta
     4321 attttgtgtt atttctggta tatttcatca ttgtgctttt tgctcttgta ttcaacaatt
     4381 ctttaggctg gttcttgttg gcctttttta ctctttatgt atcatggagc ttgatttcat
     4441 taagcagctt tctaaagaaa ctttcggtta cttgtacatt gcagccactc gtttatcgca
     4501 aaaaaagtat gcaggttatt ttttccatag caaaaaagag aactttttct ttacctctcc
     4561 ctcgtttaca aatcggcttc ccagcaatct ttgcagaaaa aaacaagaga ttttgattct
     4621 tacaaataaa ccgcaagaag tccaattgct ttggcagcca aaagaaagag gatgccatga
     4681 aacattaact gtcattttta cagcctatga tagtctagga cttttcagaa aaagtgcaaa
     4741 aagagagatc tcttttcctg tgactgttct gcctgctttt tgtaagattc aagcagaaaa
     4801 attacagaga gttctggaaa aaaaacagca gctgaacgcg taccaaagag gtcttgattt
     4861 tcgggaacat cgcgcttatc gtccaggtga ttctttcaac cgaatcgatt ggaaattgtc
     4921 tgctcatact caagagtggt tataccgtga atacgattat caagaagaag aatgttctcc
     4981 tcttttttgc ttttggggtt cgcccagtcc aaaatttgaa cctctgctag acttgtattt
     5041 ttctttatgg catttgagaa aagaaaagca gccggaactt ctgatattag gcaaccgcgc
     5101 ctttcatggc aaagaccccg gtcataccta ttttgcttct ttggaagctg gcgatgagaa
     5161 aacttttttc gcacacttga aaaaatatgc aaaaaaacaa atcgtcctct ttgttcctga
     5221 gaattctcct gaagtctcag aagctattga aacattgtca aaagatcgaa cggtttatct
     5281 tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa gacaagatat gttcattgcc
     5341 aggaggtgaa tggattgacg attaaacggg aaaaatggct gcgtggcgtt tactcctttg
     5401 tgatactaag tgtaggtgct ccgatatttg tagtagctaa tcaattgcaa aacagctacc
     5461 ttcttatcct aactattggt gtcctatgcc ttattgtctg ctgtattcct aaaaaatggg
     5521 tacgtttgct gctttctttg ccagtaatga taggttgttt gtttttatat tttccttcga
     5581 agacattttc catattatgg atcttacagt ttttttcgtc agttacagag gaattcttcc
     5641 atctatccca aggtaaactt tattatttgc cagaaaagac agcttttttt ctcataatgt
     5701 tgctgatcta tttatttatt acattgaccg tggattacga ctattggtac gcaccctttc
     5761 tttctttgat ggcttacttc atgatgctta ccgtttttcg gaaaaaagat ttactgtacg
     5821 aaatgattgc ggtagtaact gttttgttcg tgtatctggc agcaaggcaa tttttctcca
     5881 tccggttgtt gtcgagaaac actcgattcc agtcattgac tacagttctg cttatttgtt
     5941 tcttaacttt tagcgctatc ttgcctttat atcttccgaa agtacaccaa tctctcgagg
     6001 aaacgagtga accgatccga gaatatctta atgatgaagg gctttatgac acactccatg
     6061 agtatcaatt aaccggaagt gcaagaacag gcttcagtga gaatgatgaa cagcttggtg
     6121 gacctttata tgataatggc gaagttgttt ttacagcgaa ccaaaaagga aatggctatt
     6181 ggaagatcga aaataaaaat cattacaccg gttctggatg aatagaagat caggacgcac
     6241 ccatccaacc aatccaagaa ttccctgttg acatcacgag ctaccaaacc gttatagaag
     6301 acaaagaaaa cattacattg accctaaaaa aacaagcttt cttaccttat ccttatggaa
     6361 atactacctt gactgatttt tcattagcta gtggacaaac gctttactat ttgcctgaaa
     6421 gccagcgatt cattcctgtc ccgaatgaat cgacacgttc tgcaacttta caaacagaaa
     6481 ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt aaaagctgat gatttagaaa
     6541 atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa acgtattgag gacttggcgg
     6601 tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt cgaagctgtc taacagtatt
     6661 taaaaacaag cggaaatttc cgctattcca aaacggatac cgggcatact ccaaaagaac
     6721 acgattatgt ggattatttc ctctttgatt caaaagtcgg ctattgtgac aacttttcaa
     6781 cttctatggt cgtccttctt cgaacacttg gtatcccagc tcgctggaca aaaggtttta
     6841 caaatgggct cagaacaggc acagataatc aagggaatga ggaatacagc attctcaata
     6901 gtcatgctca ttcttggcca gaggtatact ttcctggatt tggctggctt ccatttgaac
     6961 caaccccttc atttgccaat ccaatgggtt cacaaccagt tgctgaaaca accgaaaccg
     7021 tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat ggcagaaact atttccagtg
     7081 agagatctga ggtcattcca tcagaagaaa caacactcac accagcttat aaaatcgtct
     7141 tattcagcct tggagtgtgt ttatccgtct tgttgttctt tctattgtta ttcagaaaat
     7201 ctctgcgctg gacaagttat cttatctatc ttcatttttt tgcttctact aatacgattg
     7261 catatcaaaa acttcttaac cctttggaaa cattggtacc gcgcaaaccc gcagaatctt
     7321 tgcaggaata tagcctgcga atagaacaag aattcaagct agatctgcgt ttcattcgat
     7381 taactactca atatgaagca gaaatatatg gacgacaagc agatccggta aaattcgata
     7441 aaaaaaccta tttagaaata acgcatataa taagaaaaac ggtatacaaa aaaattctga
     7501 caaaagggag ccgcttcaaa aaataagata aaatttctgg agaatctact tctcgaatgc
     7561 cgagaaccag ctgattgagt atcgattact cgaaaaaaag gttgtgacac gttttt
//
LOCUS       DACDIH010000103         7438 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDIH010000103 DACDIH010000000
VERSION     DACDIH010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.103"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(140..658)
                     /locus_tag="IV281_002524"
     CDS             complement(140..658)
                     /locus_tag="IV281_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP8781484.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(660..1276)
                     /locus_tag="IV281_002525"
                     /pseudo
     CDS             complement(660..1276)
                     /locus_tag="IV281_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            complement(1273..2127)
                     /locus_tag="IV281_002526"
     CDS             complement(1273..2127)
                     /locus_tag="IV281_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8781485.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(2124..3035)
                     /locus_tag="IV281_002527"
     CDS             complement(2124..3035)
                     /locus_tag="IV281_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8781486.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(3076..4410)
                     /locus_tag="IV281_002528"
     CDS             complement(3076..4410)
                     /locus_tag="IV281_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8781487.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4618..5448
                     /locus_tag="IV281_002529"
     CDS             4618..5448
                     /locus_tag="IV281_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP8781488.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5692..5941)
                     /locus_tag="IV281_002530"
                     /pseudo
     CDS             complement(5692..5941)
                     /locus_tag="IV281_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6132..6326)
                     /locus_tag="IV281_002531"
     CDS             complement(6132..6326)
                     /locus_tag="IV281_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781489.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6831..7016)
                     /locus_tag="IV281_002532"
     CDS             complement(6831..7016)
                     /locus_tag="IV281_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781490.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
BASE COUNT         2451 a         1461 c         1267 g         2259 t
ORIGIN      
        1 ttataaaatt ttataacgtt ctcatttaac aaaacttaag ttattaagac aaatgaataa
       61 acaactacgc tttcctcgaa acaaaaaaat aaagcacaaa tgcgcattta cgcactcatg
      121 ctttattcgt acaattaatt cagataacgg cagcgcatag gatgccaata acacttccta
      181 aaatagctcc tcctaaaatg tcagtaggat aatgtaaaag taaaaaaatc ctcgaataag
      241 cgatcaaaca agcaaataaa aaggctaata ttcctgcaac tggacttaat tcgaacaacg
      301 cagctactga taaaaaggct agcgctgtat gtcctgaagg aaaagaagaa gtcttcggtt
      361 tacttgtgta aaggacaagc cgacgatttt tttcataagg tcttttccgt cttgtcgttt
      421 ttttgataaa aagcaaacca acaaaaaaat agatcaaact gccgataaga atggctagac
      481 catattctct agtatcaggt atcaagacta aaacagcagc aaggagcaac caaataaaac
      541 caccattaga gatattactg ataaatttca aaaaattgat ttttcgacta tccgttttcc
      601 gttgcttggc gaacttgttg ataatactat catcgatttt agcaatcaaa cgcaacatgt
      661 tattcctcct ttttaagaac cttttcttgc gtacgatgga taaaaagcag gacttgaaaa
      721 taaaacaaga aactgaaacc taataaagca aaatagattg gtcctgcaaa ccagaatgcg
      781 acaagaagga tcaacgtgcc tgccaaatag aaaagcgcgc ttttgaaatc attaaaaaac
      841 aagaaaaatg cagtaatcca agaagcttct ttttgttcca gttgaagcaa gattttgtaa
      901 ctaaagagaa gcgcgcttaa tagaagcaaa aacaacgtag tatacatcaa tgtatagcca
      961 attcccccac cccaatagcg gaaaatctga taatcgatca atagaagaag aaaataaaca
     1021 cttgctaaac tatcttgctt tccataaaga gagaagttct cacgaaactt tttccagaat
     1081 agtgaaccga gctttttctc ttcatttttt tgtaatgata aatcatatac agaactgttc
     1141 gacttgcagt tcctaatgtg aaaacaggca agacgcacag gaaccatagt agctgtaaat
     1201 ggataagacg tggaatccat tcaatgattt ctaagaaata atcattggct tttttaccaa
     1261 tcgatcgatt catcatcctt ttgaacctcc tgtcaaatcc atcccttcaa tgaaataacg
     1321 ttgtgcaaaa aagaacagca aaatcgtcgg gatcaagaca agtgttgctc ctgccatcag
     1381 ataattccat tgtgtcgttg cttggttctg aaaaacttgc aagccaatct gtaacgtgta
     1441 tttttcttga tcttggatat ataacagagg acctaagaaa tcattccaag ctgcgttgaa
     1501 ggaattgatc gcaatggtaa tcaaggcagg tttcgtcaat ggaagcatca aatgactcca
     1561 aatatacaaa tgattcgctc cgtcgatttc agcagcttcg atcaattcat tattgatcga
     1621 taagtaaaat tgacgcatca gaaatatatt gaacgcattg ccgaagaacg atggaacgat
     1681 caggggcaaa taagtaccta cccagccgat cttggagaat aatacatatt gagggatcat
     1741 cgttacaaac cccgggatca tcatcgtaga aagaaccaaa gcaaacatca gctttttccc
     1801 aggaaaatca agcttcgcaa atccataagc aataaaagaa tttgaaagga cattaccgat
     1861 cactactagc acagtgatga ataatgtatt tcttgcatag cgccaaaaag gaaaggcggc
     1921 aatcgtatcc agatagtttt cccatacgat tttttctggg aaaaaggtcg gaggataacg
     1981 aacgatttct tccattggtt tcaatgaagt aaacaccatc caaagcaatg gaatgatcaa
     2041 aatgattcct attactgtca tgaggacata acttagtatt ttttttgttt gccgattcac
     2101 actggtcttt ttgaaataat ccatcatttg ccgtcacctc cagaataata gacccactta
     2161 ggtgcaagct tcaaattgac aaacgtcaga accaacacga tcagaaacag gatcattgac
     2221 atagccatcg cataacccat atcaaaagct acgaacgcct tgttccacat atgcagatta
     2281 taaaaaagca aagaatttgc aggtccgcct gttccatttt tcgtcatgac ataagcttct
     2341 tgaaaaattt ggaaagaacc gatcgtagag gtgatcacat cgtaaaaaat aataggagtg
     2401 attaatggca aagtaatact tttgaacttt ccccaaacgc ctgctccatc gatttctgct
     2461 gattcatata aagatctagg aacattttgt aatgtagcta agtaaagtag cattgctcct
     2521 ccagaactcc atattttcat caaaatcaac gcaggtttcg tccattgtgg atcaaacagc
     2581 caagctggtc cagggatatg gaaccagccc agaaatgtgt tgatcaatcc tgcagatgga
     2641 gaaagcagct gcatccataa gatgtagact gctactcctg ataagactgc cggtaaatag
     2701 aaagctgtac ggaatacact gattcctttg attttttgat tgagtactgt ggctaaaaac
     2761 acactgaaaa tcgtcgtcag cggtacattg aagatgacga aataaacggt gttgtataac
     2821 gatttccaaa ataaatcatc gtgaacgaac attcttttga aatttgctag accaatgaaa
     2881 ttcatcttgg atgtcaaatt ataatcggtg aaactaccaa taactgaaaa aagcataggc
     2941 cctagcataa ataccaagaa cccgataata aaaggcgaga taaataaata ggcccatctt
     3001 gcttcttttt tctttcggat agataaacgc ttcaaatttt cttgcctcct ctgtacctag
     3061 acagtcaaaa atggattatt gattttgctt gactaagttt tctactgctt tttgtgcatc
     3121 tgccaatcct tcttttggtg attttgttcc taaaagaatc tggtcaatct gttcattcac
     3181 taatgtcgtg taatccggag catctaaagg aactggtgtg atcaccgtat ttttaaagtt
     3241 ttcgtccgcc atttgataga tcatttttcc tttatccgtt aggttgtcat tattcactaa
     3301 attctcattg gctgtcttgc tcgccatgat atcaaaactt ttttcgccga atttttcttg
     3361 tacttctgga gaagaaagat atttgatgaa ttcataagag gcctcaggat cttttgctcc
     3421 gtatggtact tccaagacga atccgccacc ccacgaccaa tggcctgaac cttcttcttt
     3481 ttctgggagc tgcatcacgc caaaatcaaa atcatccggt gcattttcca tcaaactaga
     3541 gtaataattg atattttggg cacgcatggc tactaatccg gaaataaatg gatcagatac
     3601 accagaccca aattcggctt ctaatttatt gatcgtattt ttcccataat aatcttgcca
     3661 gtcttggatc cactccaagg cttccacttt atttttagta tcaatcttga ctttttcatc
     3721 tttgtcaaac caactagtcc cgttatctgc gtttagcgcc catacatcag cccctaaatt
     3781 ccataatgga taaaaaccga tgcgtgtaaa ttcttttcct tcttttttat ctagtttacg
     3841 tgctgtttct tctaattcat cccaagttgt cggtagatct tgttcactga ttccagcttc
     3901 tttaaataac tttttattat aaaaaatgac ttgtgtatct gtattgaatg ggatcgcata
     3961 aggatcacct tcatattcta ctgtatccca aagctgcgga taaaaatcct tagaagtgtc
     4021 tttttctaca tatttggaga gattagttgc ttgctgtgct tctgctcttt gggcaacgga
     4081 atttatgtcc tgtacaataa catcgggtgc atttccagct gttattgcag ataaagattt
     4141 agtccagatg tcaccccatg gctgatactt ataatttaca tggatctcgt tttgtgattg
     4201 attgaaatcc tcaatgattt cttcgatagc ttcttttcgt gcaccggatc cccaaaagga
     4261 ccagaaatca atattttttc ttccatcact tgttttttca gaagaatttg ctgcacttga
     4321 actggcatcg cttgcattac ttgatccgca tcctcctaat aaaaacaatg ctgaacttgc
     4381 cagcaaccct gtaaacaata attttttcat attttacctc caaattttaa tgtaaccgat
     4441 gccggtttta atataacgat atcattttgt tctgaaaata acaatttata aggtatgatt
     4501 ctaaaaaaat tattaatgat accaatactt tatataattc gcgagcgctt tctttgaatt
     4561 ttttaatctt tcgtagcaga atgagaatgt attaaaaata gattaggagg aattttgatg
     4621 tttagacacc agaaagaatt gcagtttgaa gcaaaaccag atcgtccgaa tccattaatc
     4681 gcgaaatatc tgcaggagct gatcggcggc caatatggcg agatgagtgt agctatgcag
     4741 tacctgttcc aaggctggag ctgccgagga gaagaaaaat ataaagatat gctgatggac
     4801 attgcaacag aagaaatcgg ccacgtggaa atgttggcga caatggttgc tcgtttatta
     4861 gagggtgcgc ctgttgaaga tcaagaagca gcgatgcaaa aagatccaac aatcggtgcc
     4921 attatctctg gaatgaatcc acagcacgcg atcgtagctg gcttaggacc tcgtcctgct
     4981 gacagcgtgg gcaatccatg gagcggtgcc tatatcattg cgagtggcaa cttgttagca
     5041 gactttagag ctaatttaaa tgccgaatca caaggaagac ttcaagtagc acgtatttac
     5101 gaaatgatcg atgatcgtgg cgtaaaagat atgctgtctg ttctccttgc aagagatagc
     5161 tatcatcaaa atctatggga atgtgcagtg aaagaattag aagaaaaaga aggttcaatc
     5221 ttagtcccaa gtactttccc gcgtgacaaa gaacgaaaag acattgctta tgacttctat
     5281 aacttctcag agggagacca aagtagtgaa ggttcttggg ctagtggcaa agcactggat
     5341 ggcgaaggcg aatatcaatg gttgaaagaa cctaagatcg aaggcgctgc accaaaatta
     5401 gaaccagtaa caccaaaaat gtatgggaca cctccattca agaaataaat atctacccgt
     5461 agaaacaccc gttttttgtt ccacacgctt tgtgagcaaa actgactaaa gtcattaatc
     5521 ataaccaccc ttcaaaaggg tggttttctc tagagctata agctcaaatc accggccagc
     5581 gcctaaagac gctggctttc atattttatg ttcaagccat attgcctttg ccagttttgc
     5641 ccacctctta aaaggtttat gtactactcc ttagccctta aaaggatcgt cttactctcg
     5701 tacactcagc ttatctaacg cactatcatg tttttcttgt tcttgaatgt actttttgat
     5761 ggtcgcttca ttttgtccta cttttgtcac atagtatcct tccacccaaa agtgtcgata
     5821 cccaaattta tattttagat ttgcacaatc tcttgatgat ctcctgaata ttggctcgat
     5881 attgattata aattattttt cttctatact ttggtgtgaa tacaatgtgg tatttacaca
     5941 tctactttgt acgggctaaa ctatttccct ttattgctcc acccgcatag caagtggttt
     6001 tgtatttcaa tcgctcggcg cgctcaatta ggtctaaaga caataaatct aaaaacccgc
     6061 tgcttcactg acagaaacaa cgggttttca gaaaaaaatg aaatgaaaaa gatctcttac
     6121 ggcaatctat tttatttttt tgatttttta taaatggaaa atgcttcaag aagtccaaga
     6181 aataaagccg tataaaaaca agaacctatg aaatctttat ttattagata ttcaacagaa
     6241 atcccgataa cactagccaa caatatattt atcagaagtg taaaataagg atgcttttta
     6301 tagaatagaa ctagtgttgt tttcatagac tctcctttct tcatatttcc aattaatcac
     6361 aaattcacgg cattactaga aaaattaaag gggcgcagaa gtggaaaaat actcttcgac
     6421 tttccctaca aaccaggcca tcttgtgttt aataactata ccaaatctaa ggtacctcta
     6481 aatctaaatt ccatttgtgg cggtgacctc caatttttaa ctcttctatc tcccatccag
     6541 tatacataac cttgatactt cgaccactta tatatcgaac acacaaatac ctatccattg
     6601 caggagtact acctaactta gttgctactt gcatctaaat aacagctgaa aagataggac
     6661 ttactcttag taaagtatct gaactattaa tttttaaact atcattttta tcctcaacta
     6721 catgcacatc tgttgcaact tcactttctg tctcatccag ataaatgatt aaggaatcgg
     6781 gaattgcaat atcttcagct gacgctggaa tagttgagct aaattagtca tcatatcttt
     6841 attataaaat aaccaacagc aaaaaataca atgaaaaaca aacttggaac taatgtatca
     6901 ttaataaatg ttgaaaaatt cagcaactca ttactgtatg attttagcag cccaaccaac
     6961 atatacccta taaaagctaa taagatggtt aggaatcctt tagaattttt actcattcat
     7021 agacacttcc ttaaataact tttattatag aaggtattgt aaataataat attgacaaaa
     7081 agtgactcaa aataaaaaaa aacttacatt atactggaat gaactgtatt gatagtgtac
     7141 ctaaaagctc tactgacata agatcgtgta atcggtaaag taattttatt aattatacgg
     7201 gatgtaatca attagagaat aaactaatca taaaaggtac ttttacattc ataattgtcg
     7261 ctttgagatt ctactttggt tcactatctg acccaattgc acattttcga tcattacaag
     7321 tattggtgtc gttagaacaa ctaaataaaa actagcggta gatttttcag aatctatgct
     7381 aatttctgta tggatgttcc agtgatggca aatttctctt ttcattgttt ttcttgtc
//
LOCUS       DACDIH010000104         7111 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDIH010000104 DACDIH010000000
VERSION     DACDIH010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.105"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(34..609)
                     /locus_tag="IV281_002533"
     CDS             complement(34..609)
                     /locus_tag="IV281_002533"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP8781491.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(764..1290)
                     /locus_tag="IV281_002534"
                     /pseudo
     CDS             complement(764..1290)
                     /locus_tag="IV281_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            1747..3258
                     /gene="gadC"
                     /locus_tag="IV281_002535"
     CDS             1747..3258
                     /gene="gadC"
                     /locus_tag="IV281_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HAP8781492.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            3271..4671
                     /locus_tag="IV281_002536"
     CDS             3271..4671
                     /locus_tag="IV281_002536"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP8781493.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(4836..6271)
                     /locus_tag="IV281_002537"
                     /pseudo
     CDS             complement(4836..6271)
                     /locus_tag="IV281_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6664..>7111
                     /locus_tag="IV281_002538"
     CDS             6664..>7111
                     /locus_tag="IV281_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HAP8781494.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVS"
BASE COUNT         2226 a         1210 c         1301 g         2374 t
ORIGIN      
        1 tgtaatcaat tatttgatta cacgtttttt atttcaatct tcgttgtagt agtggttgcg
       61 acataccgaa aggtaatctt cattacctcc tactacaatc tgttcacctg aataaactgg
      121 aataccatca acaatacgag cattcattac agccttttta ttgcaaaaat ggcaaatagt
      181 tttaatttcc tcaattttat cagcaaatgt caataatgct tcggatccct tgaagagatt
      241 attttgaaaa tcattcttca atccatagca aattactggt acttcctcat tcattacgat
      301 aagtgataga tcaataatat tctctctact taaaaattga gactcatcga ccaaaataca
      361 atcaactttt ccgtactgat caataatctc cactaattct ccgtacacat ccatttcaga
      421 agtaattgca atagctggtt tcgaaacacc tatacgactc tcaacttttc caaatcctga
      481 tcgagtatct attgcgcttg tcattatgat aactctcttc ccttgttctt cataattatg
      541 agccactttc agtagctcaa tactctttcc actattcatt gttccatatc tgtaaaataa
      601 ttgtgccatt aaatttattc ccttctttta caattcaatt attccttaat tccatctgct
      661 tttatgcagc tgctcgttta ttccttgata tgtctataac atctactatc cctaaaacca
      721 atttataagc cattagagat ggtattcctg aacatctctc atattgtttt ttgagtggta
      781 tatcatctga aaaactgaag gaatcatgaa aatagcgaca tacttatcat ttagagttct
      841 aactttaaat gcatttactt cttcagtcag cactttagtc atgttggaca tggtttgggg
      901 cgtgtagtga tgcccataca ttttctcaat caagtctgcg atttccgaca tggtaatacc
      961 ttttcggaag aggtgaatga cggtctcctc taatgtgtca ttcgtccgtc tataagcagg
     1021 aacagtctgt tgcttgaact cgccattgcg gtcgcgcgga atctggagat gaagttcccc
     1081 atactcggtc ttgaccgtac ggtcatagga gccgttacga gaattacccg tgttaaaacc
     1141 aatgcgatcg tacttttcgt aatcgaggaa agccgttaac tccgttttga gaagcgtatt
     1201 gacagctttt tccaaatgga cacggaataa tccattcaaa tcgcctttgt tcgctagagt
     1261 ctttaggatt tctgtagtaa aatcgttcat atggaagtcc tcttttctgt gaatgtgttg
     1321 tggtaactct attctacaga agggacttcc ttttttctat ttacacaaaa tattttacac
     1381 tctcaacaaa agtatcttcg attaatactt gctcaagtta tgtgcagcta cgaaaaagta
     1441 ggtaccgagg tactttcttt ttcgcagctt tttttggact tatgagaagt acattcgctg
     1501 gaagaatacg atataaaacg agctttttca ctaaagatat taatagagtg aaagaaacaa
     1561 gcttttgttg tcagtttttt cacttttcta aaaatgaaaa taaaaaattc tggaagtgga
     1621 gaagaatttt gcttgctata ctttgtcttg tagaaaagag gtagacaaaa taatgcaagc
     1681 tcaaataagc gattttccat aaccttggta ggaatgacaa attaaatagg aaagggaagt
     1741 aataatatga ataaaaaaaa gttatctcta ttcggttttt ttgccctgac agcttccatg
     1801 gttttgactg tttatgagta tccgaccttt gcaacatcac aattacattt ggttttcttt
     1861 cttcttttgg gaggtatttt atggtttcta ccagttgctt tatgtgctgc agaaatggca
     1921 acagttgaag gatggaaaga tggtgggatt ttcagttggg ttagtcaaac tttaggagaa
     1981 cgttttggtt ttgcagctat ttttttccaa tggtttcaaa ttacagtagg ttttattaca
     2041 atgatctact tcatactagg agcgctgtcg tatgtaatta attttccaga gcttaataca
     2101 gatccattta tcaagtttat tggtgtatta gtcattttct gggtactgac tttttcacag
     2161 ttgggcggga ccaatcgtac cgctaaaatt gctaaagcag gatttgtcat tggaattgtt
     2221 attccttcga tcattctctt tggtttagca gcagcctact ttatcggcgg taatccgatt
     2281 caaatggcta ttaatgcgaa agcttttatt cctgactttt ctcagctttc aactttagta
     2341 gtatttgtat cttttatatt ggcttatatg ggcgtagaag cttcagcatc acatattaat
     2401 gagctggaaa atccccaaag aaactaccca ttggcaatgt ttttactagt gattttggcc
     2461 atcgccttag atgcaatcgg cggtttctca gtagcagcgg ttattccaca acatgacttg
     2521 tctctcagtg ctggtgtaat ccaaacattt gaaacgctta ttttacattt taacagtcat
     2581 ttacattggt tggtaaaagt gattgctctc atgattgcct gtggcgttat gggtgaagtt
     2641 agttcttggg tagttggtcc atcacgaggg atgtttgcag ctgctcagcg aggtctgtta
     2701 ccagttttct tccggaaaac taataaacaa gacgttccag ttccgttgat tatgatacaa
     2761 ggaattatcg ttactatttg ggcagcagtc ttaacttttg gtggaggagg caacaattta
     2821 tcattcttag tagcaatctc cttaactgtt gtcatttatc tcgtaggata tttactattt
     2881 ttcctaggtt actttgtatt agtttttaag aaaaaagact taaaacgaac ttacaatgta
     2941 ccaggaaaaa ctgttggcaa aacgattatc ggtggaattg gttttatttt atcagtgttc
     3001 gcattgctta tctcttttgt accaccagct tcaattgcag ctagtgagca ccgaacatac
     3061 caaatcatac tacttattag ctttgtagtg actgttcttt taccttttat catttatgaa
     3121 gtacatgata aaagtgctca cacaactatt gaggaaccta ctcacgtgaa agctgaagac
     3181 gttaatcccg ctgtttatcc agctgctaga ggagagcacc acgttacaaa aaatgatgaa
     3241 catattttaa aacattagga ggaattcatt atgttatacg gaaaagataa tcaagaagaa
     3301 aaaaactatt tggaaccaat ttttggctct gcaagtgagg atgttgactt gcctaaatat
     3361 aagttaaaca aagaatccat tgaaccacga attgcttatc aattagtaca agacgagatg
     3421 ttggatgaag gaaatgcgcg attaaactta gctacttttt gtcaaacgta tatggaacct
     3481 gaagcagtga aattgatgac ccaaacgtta gaaaaaaatg caattgataa atcagaatac
     3541 ccacgaacaa cggaaattga aaaccgctgt gtaaatatga ttgctgattt atggcatgct
     3601 ccaaataatg aaaaattcat gggaacttca acgatcggct cttcagaagc ctgcatgctg
     3661 ggtggtatgg ccatgaaatt tgcttggcgt aaacgtgctg aaaaattagg tcttgatatt
     3721 caagcaaaaa aacctaacct ggtgatctct tctggttacc aagtttgttg ggaaaaattc
     3781 tgtgtatatt gggatgtgga actgagagaa gtcccaatgg atgaaaaaca tatgtcaatt
     3841 aatctagata ctgtcatgga ttatgtggat gagtacacaa ttggtattgt aggtattatg
     3901 ggtattactt acactggtcg ttatgatgat atcaagggtc tgaatgattt agttgaagct
     3961 cacaataaac aaactgacta taaagtatac attcatgttg acgctgcatc gggtggcttt
     4021 tatgcaccat ttactgaacc tgatctagtt tgggattttc aattgaaaaa tgttatctca
     4081 attaattctt caggtcacaa atatggtttg gtatatccag gtgtgggttg ggtcttatgg
     4141 cgtgaccaac aatacttacc agaagaatta gtatttaaag ttagttactt aggtggagaa
     4201 atgccaacta tggctatcaa cttctctcat agtgcagcac aactaattgg gcaatactac
     4261 aactttgtac gctatggctt tgatggttat cgtgatattc accaaagaac tcatgatgtt
     4321 gctgtttatt tagccaaaga gatcgaaaaa actggtattt ttgaaatcat taatgatgga
     4381 tcagaattgc cagttgtgtg ctataagctg aaagaagatc ccaatcgcga atggacacta
     4441 tatgatttat ctgatcgtct gttaatgaag ggatggcaag tcccagccta cccattacct
     4501 aaagacttgg atcaattaat tattcaacgc ttagttgttc gagcagactt tggaatgaac
     4561 atggctggtg attatgtaca agatatgaac caagcaattg aagagttgaa taaagctcat
     4621 attgtttatc ataaaaaaca ggatgtgaaa acgtacggct ttactcacta aaattcttca
     4681 gagaaatata aaagaagtta attaactaat aaaaggagtt gtgattacca cacagctcct
     4741 taaatctatt tgaataaata tatgatattc atttgttaca ctctcaatct tagctgttgt
     4801 tctattcatc attactctta ttgtaagtag ttcagtcaaa tttgcttgtt caattgatcg
     4861 ataacagtat gaattgtttg aaaagtaatc ggatatataa ttgttttctc actacctaag
     4921 ccttcttggt ataaagaact atcgcaaagt ataatccgct gagtacaaaa gaaagtgtcg
     4981 tctactattt ctctaagttg tttacaaatg atattactaa tagaaaagtg ttctcccaaa
     5041 atgtcttcta actgttgtcg atagtataaa tacctaaagt ctgacggagc tactatgaac
     5101 acttcaattg gagatttccg tttatgctca tttaaaagac ttaaactaat ggttagttta
     5161 cgaatagaat tctcattcca acgcagaaat aaattgtttt gttttcgcca ttgttcaaga
     5221 atttcatgaa cttctaagta caaagcgcgt tgctcttcat ttaacaagta agtcttatca
     5281 ggttgaaata tctgaatatc agcccaaagt gagcggataa acgggagaag tgctttatca
     5341 aaaagtatct gattttcttc agatatattc agagataaga taagtttttg actgagctca
     5401 atcaccaaag ggttatctgc tacatggttt tgataaacaa cgttcaagtc tttttgtaac
     5461 aattcaaagt tatcgcatag ataatttcta gagttgaata gaaagtagat ataaaggcat
     5521 tcatcttcgt catgacataa agagaaagaa agttgctcta atcctttctg tattagctga
     5581 aacaagggag tatgtcgggc aactcttttt tcttcctgac taaaatatat tgggttagtc
     5641 ttagatctct gcaaaccgat agcgatacct aataaaataa attgtttttc aactgtaggg
     5701 taacttcttg aaaaaaatgt ttttctacat aatcaatcaa ttgttgaccg ctttgaataa
     5761 ttgatttatt tagctggatc aactctgtcc gatttgtctc atagattatc gttagtaaca
     5821 aacttcgata atccttttca ggaatttcaa aatggccatc agcatttaag tactgcattt
     5881 gtttaaaaaa attaatcaac tcatttcgta agttgtaaac tttgcttaac gaaatgaagg
     5941 tagtatcagc tatattactc caattgtgtt cccctgataa ataatgtcct agtacattga
     6001 aaaagtcaga gcgctgatag atgagttgag tgagtgccaa cctcgaaaac gaagagtcga
     6061 atgcgactgt aaagctactt ctagatttct ccactgaaat aatttgctga tggagtatgt
     6121 cggtaatgca ttccaagtcg ttagtgacag tgagtgggca aacgttcaat tgatctgcta
     6181 tttcgcgaat ctgaatggtc cctttttcgt gtaattgctc acaaatgtaa acttgacgaa
     6241 aaatactttt ctccagatag gtttccaaca tagtactcct cctagtcatt ttatacataa
     6301 ctattataac tctaccaatc ggaatagtcc tgtaatatat tgaatcaaaa atataaatta
     6361 taaaataatt agatggggta tttggtttaa gtatccctta tatagtaata aatttatagg
     6421 tgtgattctc gaattaatac ccgaaaatgt gagagaattt ttatggattt tttaggatac
     6481 aaattaatta tttttgcttt tatcctgatt accagtccag tactgtcggt atcccatttt
     6541 gcagcaaaat catgttgata aagtgacaag aagactgatg agatatatta tccaaattga
     6601 gaagataatt aatcggattc ttgagtatac cctacttttt aagaaagtag ggtatacaag
     6661 cgtatgaata aagacataac tttccctgtg atggacgttc ggcttgttcc aattaccaaa
     6721 atacaggcga acgattataa tcccaatcga gtagcgtctc cggaaatgaa cttattggag
     6781 ctttctatca ttgaagatgg ctttactcaa ccattagttt gttattacaa ggaaaaagaa
     6841 gagaagtata tcttggttga tgtgcttcat aggtatcgtg ttgctaagga aagactcaac
     6901 ttaaaagttg taccggtaac agttattgat aaacccatag aagaacgtat ggcttcaaca
     6961 attcgacaca atcgtgcaag agggacccat gaaataagag gaatggaaat tgtcatccaa
     7021 agattagtaa aaaatggatg gtcagacaag agaatatcca aagaattagg catggacatt
     7081 gaagaaattt ttcgctttaa gcaagtgtct g
//
LOCUS       DACDIH010000105         6847 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.106, whole genome shotgun sequence.
ACCESSION   DACDIH010000105 DACDIH010000000
VERSION     DACDIH010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.106"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..104)
                     /locus_tag="IV281_002539"
     CDS             complement(<1..104)
                     /locus_tag="IV281_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter ATP-binding protein"
                     /protein_id="HAP8781495.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEG"
     gene            complement(110..1183)
                     /locus_tag="IV281_002540"
     CDS             complement(110..1183)
                     /locus_tag="IV281_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8781496.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            1405..2079
                     /locus_tag="IV281_002541"
     CDS             1405..2079
                     /locus_tag="IV281_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8781497.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            2084..3424
                     /locus_tag="IV281_002542"
     CDS             2084..3424
                     /locus_tag="IV281_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8781498.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(3573..4559)
                     /locus_tag="IV281_002543"
     CDS             complement(3573..4559)
                     /locus_tag="IV281_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP8781499.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            5283..6314
                     /locus_tag="IV281_002544"
     CDS             5283..6314
                     /locus_tag="IV281_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8781500.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            6423..>6847
                     /locus_tag="IV281_002545"
     CDS             6423..>6847
                     /locus_tag="IV281_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP8781501.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
BASE COUNT         2220 a         1247 c         1171 g         2209 t
ORIGIN      
        1 tctccttcct ttacataaaa atcaattgct ttcaaagctg agacctttgt atgaccagaa
       61 ccaaatgttt tggtaacttt tttcatcgtt aatatttttt tcatttttat taacctccta
      121 ttgctgtaat tgggtctact ttggttatcg tacgtatcga aaacaatccg cctagcattg
      181 cgacaatgat caaaatccca ctatatagca accattgtga ccaaaaaaca gcaaaaggca
      241 tcgcgcttgg taaaatcaga ctagtcaaat aagcaaacaa aagagcaagc agaacgccta
      301 ccaccccaac aataaaggac tgtgctacta aagatttagc gatgaagaaa tttttgatcc
      361 cttgtgcttt cattactcca aaaatagcag tcttctgaag tgtcatcacg tacatgaaaa
      421 taccgaccac ggcagcaacc actaagaaca aaaagtatat catcgcattt aaggtcatat
      481 tttgggcaga atagccagga atactttcaa tgaatgtccc aatagttaat aactgtagac
      541 ccttcgatgc tttttctttc gaaattttag ctgaatcctt tattacgatc ccattgatcg
      601 gctgttcttc ttcagaagca aacggctgtt gtccatactt caaagcagtc cacgtatcta
      661 gggacgcata gatgacggga gaaactgtgt aataagtttc aggaaagata ccgacaatcg
      721 tcaaaggatc gtcgtaactg ccaattttga tcgtatctcc aattttatag cctgaatcag
      781 ccagattttg agagatgatg atttcattct ttgttttaaa ttcatgccct ttcgtgagtt
      841 ttggtaataa aaatgcttct ttcgttgtgc cgaaaactgt aacattttct ttgttttttc
      901 ctttgattgc tccgctgtac aatccgattg gtgctttctc tcctccttca atgtatttca
      961 gatctcctct tgttaattgt gaagctgcaa atacttgatt tgcatcctcc gacaaaacaa
     1021 tctcttgtgc gtcccaatca tcgattgctt ttttaaactc ttctgaaagt ccattcgcta
     1081 gtcctgacaa catgaatacg acatacgcaa tcaagaacat aataccgacg atcaagccat
     1141 atcgtaattt agaatagcga atttctttga tagctagaaa catttgtttt tcctccctat
     1201 gatagattga tagtgacaaa atgtcacttg ttgaatagtg taactctaaa gtgacaattt
     1261 gtcacttgtg ttgttttttt cttatcaata aattgtctaa gacatctttc gataaaacct
     1321 taacatgagt cataaaaaat ttatttaagc cttatttatg ctttccatga taaactaaag
     1381 tgaccaaatc aatgaggtgg gaaaatgatt aaactattag tagtagaaga cgataaagta
     1441 ttatccaata atataaaaga aatactcgct gcaatcggcg agataacaca agtttatgat
     1501 ggtagtgaag gactgttcga gttggaaagt ggagtttatg atcttgcaat cttggatttg
     1561 atgatgccgg aaatgaatgg atatgaagtg ttagcaaaaa tacggaaaga aaaaatacag
     1621 actccagtat tgattttgac tgcaaaagat agtttggaag ataagattga aggatttgaa
     1681 cgaggtgcag atgattatct aacaaaacct ttctatcgag aagaactgat catgcgcgtg
     1741 aaagcattat tgaaacgctc gctaggtctc tttgatgaaa atgttctgaa gtataaggat
     1801 atcagttgtg atttaaaaag caatgaagtc ttgtataaag aacaagtttt gcccatacaa
     1861 ggaaaagagt ttgaattgct cgtttatttt ttacaaaata agggaatcat tttgacaaaa
     1921 gagcagatat ttgaccggat ctggggattt gattctgata cgacgattac agttattgaa
     1981 gtttatatga gcaacttacg aaaacatctg cgaagtactg gattggataa agaaatcaaa
     2041 accttacgta atgtcggtta tatactgcaa agggtgtgat gcaatgaatg gagatattac
     2101 acgaaagcaa cgtagtcgct tttttgcgac aaatgtagca gcatttgctt ttatcttttt
     2161 tcttttggga ttcattactt tacagatatt aaaccaatct gcatacagag aaacagatct
     2221 gtcattagag aaaataaatt ttgattctcg aacagtacaa ttagagatac aaagatatca
     2281 gcaaggcgat ccctttttaa acccgactca aacggaacaa aacagaacaa acatggaaca
     2341 gccattaatg gaaccatcaa atgaccgttt taacactcaa gtgatcttgt ggtctaaaaa
     2401 tggtgagata ttgaataaag cagctattgg tgggcgtttc aaccaaatca aagaattaaa
     2461 aatggatacg aaaaatctga ataccatcca aaatattgaa ttaaatgaag acagtggctc
     2521 atctgccctc tctttccgtt cgattacgaa aaaagcggag acgaatcaag atggcgttgc
     2581 ctacatccaa attttagaaa atacaaatca aatcaagaat tcattagcga cttttcgcac
     2641 gattatgatt ttgtgtatga ttgttttttg gattctctct attggtatca gttattactt
     2701 gtctagtcta agcatgcgcc ctattattct ttcttggaga agacaaaaag aatttgttga
     2761 gaatgcttct catgaattgc gcacaccgtt aacgattatt caaaatagtt tggaacatct
     2821 ttttaccaag cctaatcata ctattattga agaatccgaa agcatcgctc aagcattgtc
     2881 agagacgagg cgcctcactg gattgacttc ggatttgctg acaatcgctc ggaatgattc
     2941 tgatcagcag ctgctttcaa agcaaatgat caatacacaa gaatatatcg aacaacttgt
     3001 taaaccattt caagagatgg ccatcataga tggaaaagag tttattctcc ataacgatgc
     3061 aacattagaa gtaccaattg atgaaaaaaa aatccatcaa gttttggtta ttttgctgga
     3121 taatgcatta aaatatacga atccagaaga taagatcatt ttggattcat ctgttaacca
     3181 taaagaatgg ctgattcacg taaagaatac tggcccttct atcagtgaag aagatcaagc
     3241 aaggattttt gatcgatttt atcgggaaga tcaatctcgg gcgaaagaga ctggcggata
     3301 cgggcttggc ttggcaattg caaaacaggt aatcgaacag catcatggac agatttctgt
     3361 atccaattat cttccacaag gagtcgattt taaagtccaa ttgccgttga agcctaaaaa
     3421 ataatgctta tccctaaaaa agggctgtga caaaaatctt gtcacagccc ttttttgttg
     3481 cgaataaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt ctttaagctg
     3541 accacttctg tcacaacctc tttagcttac gcttacgatt ttttattgcg gtcttcaatg
     3601 tgtacatcac cgcttgtgtc aatatccaat tcttctcggc taacatcttc tgtcactttt
     3661 tcaacatctt catgttcttc tttatgaatc ccaacttctt cacgaataac tgtatgtttg
     3721 cttacatcta cttgctcttc tttgattggg atagtaatcg tgtcatcttc caatttttcg
     3781 tctgttccct gtgaagattg atctgttact ggtttgcgtt cgatgacgat ttcttcacgt
     3841 ttgaccggta cttctaccgt ttcagtatcg ttgaccacat gtttgtggat gtcgacttct
     3901 ccagttgtca catcatgtgt atttacatct aatttttctt cttttagttg aattgtatca
     3961 tcatctagat ttggtttgcg atcgatgtct tcagtcttgc ttgtacccat tgtatcagtc
     4021 tcgccaggta cgtccatcgg atcagttcct ggtgtcatat tcaatggatc cgttgtattt
     4081 tgaaagcctg tcgtgccacc tacgtccatt ggatctgttc cagtgccagt ttctaatcca
     4141 gtccccattg tgcttgctgg gctaacagta tcttgtgtat ccattgtatc ttctgttgtt
     4201 tctggctgac gatagccttt tactgtgatg acaaattttc catcagaaat atctcctcta
     4261 tattgatata aaggatcatt ttctggtgta gccgtctcag aatcataggt atcaaaagaa
     4321 aaagtatctt ttattttttc ccataaagaa cggtcttctt caccatccat ccgattttca
     4381 tctgtttcaa catctatacc agacaatccg tcaaatccta gtctagatgc tgcttcatca
     4441 gtagtataaa taacgatatc atctcgttca tacccttcgt tacgcaaccg ttcaattact
     4501 gataatgctt cttccctcgt actatagctt ccgatgaccg ttaaattttt ctcttccatg
     4561 taaattcctc ctagcaaata gatttatgat tggtatattg ttacttataa atttattcta
     4621 taggagacga ttgttttcta caaatattta gattggaatt tgattaaaac aatttaattg
     4681 aaatttgaaa acatttatgt taatgaaagt catattctaa taaaatgtca tttgtttcta
     4741 tatgaatttt tatgaaaaat ttcatataat ttccactcgt tgtgatggtt taatttatcc
     4801 aatccatcaa taagcaaaca acttatataa aaaaggatta attctcaata taattgctcc
     4861 taccatagaa taaatcttca tgtaaagtaa tgaagtacca actatttcta atacttcttt
     4921 aagaattatc ccaacaatat cgttagacaa accattattg aataaatgga gagccaatcg
     4981 attctgaaaa aatgttttct aatttttcgg aaataaaaga aagagcctcc tgccattaga
     5041 acgaaccaaa tccatttcat tgctcatgaa gtaaccacat agctaaaaag aggataaatc
     5101 aattaaaaga tcaaaagaat gactgcacta ataaaataac taaaaatgta tcttcttact
     5161 tcatatcttt tagctagtac ttagttaaaa atttgcttcc taagatcaag acaaactgtc
     5221 tatcttcatt tgatgaaatg cggtaaaata tagaaaatga acactattgg agggaatttg
     5281 taatggacag caaaactaga aaacaactgc aagaaaagtt aaagcaacgt acacacttgc
     5341 tcccttccgt ctttcaacag tttttagaag aaaatcgttc ttccctttca ttaggcagtc
     5401 gctatgaata cgcaaaagat tttcatctgt ttactgatta tttgagaaaa gtattttctg
     5461 aagaagtgga tgatcagctg attatccatt tagaaaaaca agattatcag aatttcttag
     5521 ctaaaatcta tcgacatact cgttcgttcc aaactacagc gaaccaagaa acggagcaat
     5581 tgtttgaaaa tagctattac gggatttcaa ggaagcaata ttcattaaat cattttttac
     5641 gatttttgta tcaaaaagga ctgattgaag aagagatttc tcttttgggt acaagtttac
     5701 cggaaacaac caaagcagct ccgcgatata tcgatgcaga acttttcaaa gattttgagc
     5761 atttgtttat cccgattgaa ggctttcaaa caagcagaga agctagcttt gaagaaaaga
     5821 atagaccgag gaaccttgca attactgcca ttcttctgta ttgcggatta aagatccatg
     5881 aagtagtaga acttaaacgt aaagatgtat ctttgaccaa gcagattctt aatctgaata
     5941 gaaaagaaaa cagactgaat aaagtaccaa taccaaaaga agcgatgcca tttctggaga
     6001 actattttaa gttaacagca caaaatacgg acgaaaattc ttttgttttt cgttcctcac
     6061 atgatcagca aatttctcct cggactattc gccaaatttt gagcaaaatt acagtatata
     6121 aaaacgtatc gccagaacta tgcagaaaaa catatcgtta ttatgcagaa ctatatttag
     6181 atgatgctga cgcggtaaat tatttatgtg gtaatcgata cctctcatcc cattcttttc
     6241 aagatatctg ggaaaagatg gttgattttt cttatcacga attagcggca ttcgttcggg
     6301 tttcttccat ataaaacaaa tgtatgatct ttttttcaca ataaactatg ttattatata
     6361 aaggattatg ttacgattat cttgaatttg tatattactt aacaaaagat aatggagtgt
     6421 gaatgatgga gaccataacg tattcccctt tgaacttatt tacgaatttt caagaagctg
     6481 cagctaactt ttccgagacc ccgattatct ttgatgagcc actttcagct ttcccagaat
     6541 taggacttga aacaacttac aaaaaatgct gcgaaagtgt gttgaaacgg gcttatcaat
     6601 tggctcatct tggagtgaaa tccggagaca aagttattat ttataagagt tccgcttttg
     6661 acacatacct tctagctgtt tctgtgtctt accttggtgc agtgcctgtc atgacttctt
     6721 atcatctacc aacaacaaca atggaagtat ttatcgatcg tttagaagac cctttcattt
     6781 tatttgatga tgagacaaaa aagcgagtga gagaaatttc aaatggaaca aaatcaaaac
     6841 agattcc
//
LOCUS       DACDIH010000106         6711 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.107, whole genome shotgun sequence.
ACCESSION   DACDIH010000106 DACDIH010000000
VERSION     DACDIH010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.107"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            111..989
                     /locus_tag="IV281_002546"
     CDS             111..989
                     /locus_tag="IV281_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8781502.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            1180..1332
                     /locus_tag="IV281_002547"
     CDS             1180..1332
                     /locus_tag="IV281_002547"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781503.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(2356..3168)
                     /locus_tag="IV281_002548"
     CDS             complement(2356..3168)
                     /locus_tag="IV281_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8781504.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            3331..3438
                     /locus_tag="IV281_002549"
     CDS             3331..3438
                     /locus_tag="IV281_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8781505.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            3496..4563
                     /locus_tag="IV281_002550"
     CDS             3496..4563
                     /locus_tag="IV281_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP8781506.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            4705..4908
                     /locus_tag="IV281_002551"
     CDS             4705..4908
                     /locus_tag="IV281_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8781507.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            4968..6170
                     /locus_tag="IV281_002552"
     CDS             4968..6170
                     /locus_tag="IV281_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8781508.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
BASE COUNT         2209 a         1038 c         1387 g         2077 t
ORIGIN      
        1 tactggtatt caacagaagc ttcaggaaat actaaacgtc atttcactga tcgagaacgc
       61 ttgccgcgtt aatgaagtgt ttttcacata ttcgaaggga ggaacctatg atgatatatg
      121 gggcaattga agctggaggg actaaatttg tctgtgcagt cggtactgag gaattaacga
      181 ttttggaaag agtcagtttt cccacaacaa ctccagttga aacaatgaag caggtcattg
      241 atttttttaa accttttaag gaacaattgg caggcatcgg ggtcggctgc tttggtccaa
      301 ttgatattca atgcaaatca acgagtttcg gtcatatcac atcaacacca aaacttgctt
      361 ggcaaaattt caattttgta ggaacgctaa agcaatattt caatattccg attgaatgga
      421 caacagatgt caatgcggct tgctatggtg aatatgttgc tggttctggc aaagggttag
      481 caggtgttgc atacttcact gtgggaacag gtatcggagg tggtgccttg ttcaatggga
      541 ctttcgtgga agggttcagt catccggaaa tggggcatat gttgatcaag cgacatccaa
      601 aggacaattt ttcaggaaac tgtcccttcc accaagattg tttagagggg atggcagcag
      661 gaccagcgat tgaaaaacgg ttgggtgtaa aaggccagaa tttattggct gacgattcgt
      721 tttggcagat tgaagccttt taccttgccc aatgtgcata caatacaaca ctgatgtttt
      781 cgccagatag aattattttt ggcggtggtg taatgaaaca agaacacatg aagaaaaaag
      841 ttcaggataa atttgttgag cttataaatg gatatgttga aataccacca attgatagct
      901 acattattac gcccgaatta ggtgataatg ccggaataat cggcggatta gcattggcca
      961 gaaaagcggt aagaaataag caaccataat taatgaatgt ttcattgata ctcatgtcaa
     1021 aagtatttaa ccgagtaatc aaatgatcaa atgaagttgg ataaagcgaa aatgaattca
     1081 aataaaaagg ctctaagtca aatcgtgttg ttttcattaa atcactgaaa ggaaatgctt
     1141 ctgcgtttcc ttttttcttt ttgttgtgcg ctgtatacct tgaggatatt cactggtggc
     1201 gtacacttaa atagtaatat attgtgttac tttatgtctg tggtaatagt tatagttgct
     1261 taccaatgta tgttcatttt tcgaaatgtt gacttcataa tccttggctt aatgtttgaa
     1321 tttttttggt gatagatttt ttcagccgta taaaataaat cgtaattcta ttatagagag
     1381 ttgtgattat aaaaagatag caagaaaaaa aaagaaagtt tctatacatt ttaaatattt
     1441 tattcctatt tctattgcta aaaaagtaga aattctatat ttacctttaa ctctcgcttc
     1501 tcttgcaaat gtagttagca ttttaatcga taataaactc aggtagttat attaccttga
     1561 aatgattgat aattttgtca cttctatttt ttttgcaata aattttttat tttagtgaaa
     1621 aataagaaac tatttctttg aaggcgataa gttagaaaaa tattattgta gtcttttctt
     1681 tattaggaat catcactgtt gtatcttatt accttttctg tttttcttat tagagaaaaa
     1741 taatttgaga ggtagatagt ttttattgga ggaagtaatt atgtatattt ttgaaacaaa
     1801 aatttttcat tgcaataatt gcaacaaaga aatttcatcg ggggaagagg tttggacaaa
     1861 aatggaaatt tccgccaaaa gtaactgctc ctcaactgaa atcaattaaa gaactagaat
     1921 ttgagaatgc actggtaatt tgtatgacat gtactgatga agtatttcga aaacattttt
     1981 aaaggttgag atgtatatag atttaaccaa tcatttatct ctctcggaaa aggaatatta
     2041 actaaccagc gggttatcaa aaataggtag gaaaactgta cttaatggct tatttcgcgc
     2101 tgattatgag aatatcagtc tgtttgatta atttttaggg ttcaaaatac agagacaaac
     2161 tccaggtcgg gtttcgccac aagcgaatta cccgatctgg agttttgtct attgcaggaa
     2221 ggcgtagccg gactgcagga ataaagcccc cggttctaac agggggctaa caaagaacag
     2281 gaaactgtga caactaccca ttggggagtg tcctagcagt attttctttg tcacgtgctt
     2341 atcaattttg tcacctcatt ttgatgatgg atacgctgca tttaacaggc gttctttctc
     2401 gacttccatc acgaatggta gataaagata ttgctgttct agtagatatt gattgtcctc
     2461 tgtgtactga taagaagcgg tttcttctga atagaccaac ggggcaattc gcacatcgct
     2521 ggtcaattga ttaattgagt atagaaggat ggctttctca cctagttcct tgggtaaatg
     2581 tttcccaaaa taggaatagg atttttgatt tgctaaatag acgagtatgc gatccacact
     2641 aaagttggag aaggtaaagg gcatcatgtg cagaatatga ttttgattat catgttgcaa
     2701 atagagctgg gcttccttac caaaattagt tgaaccaaga taaagctgct ttttttcatc
     2761 gatacattgg aaagtaaact ggtcttgttc agttggtgga atgagatttg taataaaaaa
     2821 ttcaatcatt cgatccattt ctttttcact aacaaggtca gcgtgtttga tttcgtctac
     2881 tatgagacct tcaatcttag gatcaagctt acctgccttt ttttggagca tttctaaaat
     2941 gtattgattc tcttgatcac catacaaaag atattcattg gtgacatgac ctatctctgc
     3001 gatttggtga agggtttcgg tttttggaag gttgatccca cgttcccagt tattgactgt
     3061 acttcgcggt aaatcaccaa ttagtttgcc aaatttctcc atactcaact ttaattcttg
     3121 gcgaatctgc cgaattcgat tgccgactgc ttgcttgtct atctccatga aaactcctcc
     3181 tcaggataag ttcttaaatt tagtatacgt gtgtacttaa aaaaagtcaa aacatattga
     3241 cttaaaataa atacaggcgt acaataaaaa tgtacttaaa ataagttcaa taggttcttt
     3301 gacaagtaaa tagcagaagg aggtttgtcg atgaaccaag ttaatcaaca tgatctcggt
     3361 gaagccattc gagtttctcg agaagaaagg gggtggacac aacgatattt agcagaaaaa
     3421 gtggggattt cgagatagtt attatcgaaa gtagaaaaag gaacgagaca attaagtgaa
     3481 gaaaaattga atcttgtgtt ggatagtttg caagaagagg tcgttccagt taaccgtgtt
     3541 ttgattgatt atctaaccat tcactttttc tcgaatcaac atttaaagct aattgaagag
     3601 attattgaaa tgcccattga acgttttgag gaactggatt atgcgccaaa gggctatatt
     3661 ggccaatacg tgtggaatca agtaatcacg attcggtatt ccattgacga tgcggttaaa
     3721 ggaactgtga tggagttttc aggtcaaggc tgtaaacact tagcaagatg gctaaaaaca
     3781 cgaaaaacaa attggcaaca gttttttcgt aaggtattag attatcaggg gaactttaca
     3841 cggattgatt ttactttaga tgattttgtg gggagtctca gcattccaga acttaagcat
     3901 aaggtaatac taggccatgt gtggaccact tttcaagtga gtgaatctca tggcggtacg
     3961 gatatcataa acaatgaatc aaatggtgag accttgtatt taggctcaaa gaagagtcag
     4021 tgtcgttttt gcttttatca gaaggaccat gaacaacgga agcgacgagg aattccttta
     4081 gaagaggcaa aagtaaagaa tcgctttgaa ctacggtatc gaaaagagaa agcgcaaagc
     4141 ttggcaaaga ttatttcaaa aacccatgat ttaaccaaac tgttctttga gttacttaat
     4201 ggagcaattt gtttttatga ccgtgatcca aatgatctag gtgcaaaagt tgatgccaaa
     4261 tgggcagcct ttattagcaa ttatggcgca atcaccattt ctttagatac aattcctcaa
     4321 agttttgaga aaactatgag ttggttaatt catggtgttt caccatcatt agtttttatt
     4381 gcacaggtcg atcaaacgtt tcattcttcc ttgttgcctt tgattattga acaaggagaa
     4441 atgaatccaa gacagatcaa acttctccaa aatatgaagg cagacccgga gtattatcag
     4501 gaggaggttg agtattatga acgaaaatta caaagtatat caaatgagaa aacaagctac
     4561 taaaaaagca aagtactgct tacacaacaa gttgtgcaac gtactccgcc tcgacaagac
     4621 gagtataaca caaccatcga atgagaaaca atagattata aagaggatta ttttaaaagg
     4681 agtaaagaat caggaggtga gagaatggaa acaaaatgtg atttcatggt aaatcgtgca
     4741 attttgatag agatgggctt taaacctagt caagctgcac gaatgattaa agagagcaag
     4801 gcatatcttg caaggattga aggtattgat ttttataata atcgccaagt tggtgtggtg
     4861 ccatcccgag taattgaaca tctttttcac attcaagttg cggaataaac agtacaagcg
     4921 cggtactatt aaagtagctt ttctcaagta cagggaggaa tggaacgatg ccaaaaatta
     4981 aaaatattta ccaagataaa aagacaaaaa aatggttctt ccgagcgtat ctaggtatag
     5041 atgaaaatgg aagaaaggtt cagaagacta aacgaggtta tgcttcacaa aaagatgcca
     5101 aaattgctta tgacaagtac atggaaacac atgattttaa caagacaatt cctaatcaac
     5161 tgtgttctac caatcaaatg actttcgaag agttttacaa ggataggttt gtaaaatggt
     5221 acgagagaca ggtcaaaagt caaacatatg agaatgcgca attcattttc gaaaagcgta
     5281 tgcagttttt ctatcatttt cgtgttcgag atattacaag tcacgatatt gaggattggt
     5341 tatttgaact aagccagaca gagtcacgta attcacggaa aaaagttaat ggcgaaacgt
     5401 tatcaaaatc ttatatcaat cggattcgag ggcacctaaa aattgtattg gatcgggctg
     5461 ttaaagaggg ggtgattgct aaaaatcccg ttgaagatgt ttcttcgctt ccttctgaga
     5521 ataagaaagt tgagttttgg gaacacagtg agtttcaaaa agtgatgaat gaattgaagg
     5581 atgataagat tcaaacccac catcggaaga ttgtgtatga aatgctcttt tatactggca
     5641 ttcggattgg agaacttgaa gctttgtctt ggtcgaatgt agattttgag aaaaacacga
     5701 taacgattga gaagacattg atttataata caaaagatga ttggtacttt tcgactccca
     5761 aaactaaatg tgcttatcga acgattgggt tggggaagaa gctttcctta aaactcagac
     5821 agtggtatga cttgcaaaaa cgtattggaa attttgaata tgttgctcag ttggacggaa
     5881 catttacgcc accttattct tttgcgaact ggttaaaaga agctgctaaa aaagcgggtg
     5941 tcaaaccgat taagcttcac gcgctacgac attctcatgt tgcatttcta attgagcaga
     6001 atattcaacc gctatctatt caagaacgat tgggccatgc gaatattcaa atcactttag
     6061 gaacttacgg tcacttatat gcaaagtcag atcaacaagt ggtaagtgcg attgataatg
     6121 ttcaagaggg cgctattctt tcaaaaaatg acaatttatt accagtctga gttttccacg
     6181 gttctaccat aagacaaata aatgcttttg caccagtgtt ttcagtcgaa ttgctcctgt
     6241 tcaagatgta tcggaaatag cgagaaaata agcaaatttg cataactata ataaaaagga
     6301 cttagttttg atgggaaatt tttcaaaatt aagtcctttt ttatgtggtt gaaacgggta
     6361 tgatcatttt aaggaagtga tctaagatga aaggacgact gaacaattat catattagaa
     6421 aagctttatt gtattccaca aacaaattac taagaataac aaattagttt ttgtttgttc
     6481 ttattcccta ttgacttttc tttcaaacat gctattctaa ttttattcta atagtatccg
     6541 atgagaagga aagtagacag aaaatggttc aagagaggct ttggttggtg taaaaagcta
     6601 gaagttgact gtcgaaaatg gccttagagg attttttgtc gaaaggtagg caaaaacgaa
     6661 cagtctttcg ttaccaagac cactgatttt attgtcaggc tgagagaaag a
//
LOCUS       DACDIH010000107         6632 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.108, whole genome shotgun sequence.
ACCESSION   DACDIH010000107 DACDIH010000000
VERSION     DACDIH010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.108"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            148..2823
                     /locus_tag="IV281_002553"
     CDS             148..2823
                     /locus_tag="IV281_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAP8781509.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3005..3244
                     /locus_tag="IV281_002554"
     CDS             3005..3244
                     /locus_tag="IV281_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781510.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3260..4633
                     /locus_tag="IV281_002555"
     CDS             3260..4633
                     /locus_tag="IV281_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8781511.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4637..6271
                     /locus_tag="IV281_002556"
     CDS             4637..6271
                     /locus_tag="IV281_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8781512.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6434..>6632
                     /locus_tag="IV281_002557"
     CDS             6434..>6632
                     /locus_tag="IV281_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781513.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2322 a         1071 c         1332 g         1907 t
ORIGIN      
        1 ttgtgatttc caaaatcaca acttttttct tttgtcaaga ttttgagtgg gatatatttt
       61 tgtagaactg aagatcatga aattatgaga ggttggaggt aatgtagtca tacaaataaa
      121 aattttttag aaagtgagtg gtaattaatg aaaatgaatc acacttcaat tcagaagcat
      181 ttacaactgt taaatacact ggggatttct tatgttaatt tgagaacttt ttctacatta
      241 aacaagaaaa ccgtttctca tattttttct actgcagatt ctcaagcaat tattaactat
      301 ttagaaaaac agaaattgtc tgaaggtaca gtaaacataa cttataatcc tattaaacaa
      361 gaggtgatta cacaaaaaaa gcattctgtg agagacaatg atattgaaaa aattcggttt
      421 gtatttattg atattgaccc taaaaggaaa gagggaagtg caacagatag cgaaaagaaa
      481 aaggcagaaa atgttatgga acaagtagaa cgttatttga aagaaaagag aatagaatca
      541 tttgtaaaag ttgatagtgg gaatggctac catatttttc ttcccataaa tgaacaacca
      601 aataatcacg agacgattct tactattcag aattttcttc aattacttca ccgaagattt
      661 ggagtagaag atgaagttga tattgatact tctgtttata atccttcaag attatgcaaa
      721 ttagttggta caccagcggt caaaggaaag tccacaaaag agcgtccgca tcgtttatct
      781 agtttccttt ctattcccga taacatagat agaagtaatt gttttaaaat tcttgaacag
      841 atagttgaag aaaattccat gatgtcaacc tctagagtaa aaagccatcc atcttttaga
      901 gaagtgttac cttttattca agcagactat aaacaatggt tagctcacta tcctgaatta
      961 acttatacca taaaagaggg cgatcataaa ggagttacct tacttatatt tgacgaatgt
     1021 ccattaagaa aacacacgaa taattctaat ggttcttgtt tgtctgttac ggctgataat
     1081 aagattcgat ttcattgtct gcatgcttcg gataaaaata aagatattca agactttgta
     1141 aaaaaatatc cgttgccaaa acctgtaaat ccttctgtag aatcattaga atccaatcaa
     1201 gaatattatt ttgaccagtt taaactgaat caagaaggag tatttttggt gaaagaaggt
     1261 caagaagaaa aagttgcttg tccgatgttt attaagcaaa tacgcaaaaa tatcgaacaa
     1321 aaagtcatta cacttgttct tgcctattat gtcgatcatt gttgggatga aatagaaata
     1381 gaaggtggat gtttacaaac taataatttt aaacaattag caaaacatgg cttaatcatt
     1441 aaagctagaa gagaagctga agtcgttgac tttttacaga ttcaacggga gaatgctcca
     1501 atcttttttg aacatgaatc tcttggctgg aaaaaagaaa atgaagcaat gatttttcag
     1561 ttacaagatt cttttggacg tgaaattcct tctgtattaa ctaaaaactc atgctaccaa
     1621 ttaacaacta atggtacaga agcggaattt gacgcattgg ttcataagta tgttttagga
     1681 acgaacttag aacttgcttg gtcgattggt attgcttcta ttgtgttagg ctatttaaac
     1741 gttacgaagg ctaatacttt tttgagttta ctaattaacc tacagggcaa gagcacaacg
     1801 ggtaaaacca ctacgttaca ctttattgcc tctttatttg gaaatccaac tacggttctt
     1861 agaaatatga atgctaccaa taaagccatt ataaagctag catcaaacaa tcagggaggt
     1921 gttccactaa tactagatga gttaggagca gcaactcaaa ataactttag ctcgttgatc
     1981 tatcaattag cttcaggaga agaaaggcta agattagatt ctaattgtca gcttatgcca
     2041 caagagactt tttctgtttt gacccttacc tcttctgaag agcgtttgga aaattatttc
     2101 aatgagaaat taactggttt gaaagttcga tatatcgaat tttctgatat caagtttaca
     2161 aaggatgcga atcacgcaga gaaagttaaa agtctttctt ctaatacttt tggttggggt
     2221 ataaagttat ttcttttgaa actctttgaa caagggatta caaaaattga agcaactttt
     2281 gtcgaagctc aaaaaaaact atgggctgtt ttacctgaac attctttgaa gcaacgcatt
     2341 ggtaataatc tagctttgat ttatagtgga gccctcctaa ttcaagatat actgggctat
     2401 cctttacgat tagagcatat acagacggtg cttattaatt cgtacgaaca gttaattgta
     2461 gatactagtt taggaagtga gcaaacggta tttgaccgag tgaaaacatt gttggtgatg
     2521 aatagccata aatttttaac aaacagtgac aacaaacata cgcccagtac gatttgggga
     2581 aaagtatcaa ttcaaaaaaa tggggatatt aaggtaaata tttttcctat cccgtttgag
     2641 gacttgttaa ggcgagaatt tcaagtaaag gatctaacct atctttttag gcaattgctt
     2701 gattcgggta atatgtgtgc agaaaaaggg agaagaacga aacgtattca catgaatcgt
     2761 caagttcttc caacatacga gattctatta ccttcttcac tcaaatcgta ttttcgattg
     2821 taatttagaa taaaccaaag tatgaaagtg aggcaccatg ttatggaaat atttcttatt
     2881 gatttagcaa aacaactgat tacttatctg tatgatagcg agcaaaccca aacggaagta
     2941 aatgaagctt gatctatgct tcatttactt cattcaatca aacatgaaag aaaggaaaat
     3001 tctaatgact aaaaataaaa tgacactaaa agcagaggtt cttctgtata ttcaagaaca
     3061 tttttcaaat caagcctttt ttacaaaacc aatttacttg gatttcgaaa taagaggggt
     3121 atctgctgga tctatcgggg gtactcttca agcgttgaag aacgaaggat atttagaaaa
     3181 tcactttgtg caacgttcgt tcaatagaag agtcgtgaaa aaatggtatt tagttcattc
     3241 atagagaggg gaatcaagca tgcgtgtggc ttgttatgat cgaacgtctg tttatgaaga
     3301 agcgaaaatt agtaatgacg ctcaatatga tgcagtgaaa gagtatgttt tagagaaata
     3361 tgaacaaatt ccccaacgat tttcagacaa aggagaatca ggggaaaaag cggagcgtcc
     3421 agcttttcga cagcttagag cttgggtttg tgcgtcagaa gaagaggggg tgatctatgt
     3481 ttggcgttat gatcgattgg cacgtcaagc caaaatagcc ttagaattta ttgagttgtg
     3541 ccaacaacat caggttcaag tgtattcgat ttctgaacct cttcctgctt ctttctcaca
     3601 cgcgtcaacg aataaactat ttgtgcaatt attgttcact tttgcagaac aacaacgaaa
     3661 cgtcattatt gagaatgttc gtgccggact ttcctataaa aagcaacaac ggcaatattt
     3721 gtcttctgcg gtaccatatg ggtatcgttt agtgaatggg aaaatccaac aagaagtaca
     3781 cgaagcaagg attgttcgct atatttttca actttatttg accaaaaaat atggctacaa
     3841 aaagctttgt caacgattga cgcaacaaaa gcttttcttc cgcgaacgtc cttttcagcc
     3901 ctatcatatt tatagtatcc taaaaaatcc attgtactat ggcgaaatca aaggaggatc
     3961 tttggggaag tatctaggaa cctttgaacc gattctctcg aaagcaacct ttttacaagc
     4021 ccaagagatt cgccaatcaa ggtgtacagc taaaaaagat acgtatctgt acttattacg
     4081 tcaaaaaatt cggtgtccct tctgtggtcg tcatctgagt tcgaagtatc aatggaatac
     4141 aaagaagacg aagacgcttc attattatca ttgtaccgac cgaagctgcc aaggaatttt
     4201 tcttcgggca gaaagggttg agtcagaagt attggaaaag atacaggctt tttttcaata
     4261 cgaaggcgtc tatcaagcct tgattgccca attaacgcaa caattagccg aaaagcgaaa
     4321 agatgaaaaa cgcaaagtaa tccaacaaaa gaaacaagaa gcacaatgga ttaaggcatt
     4381 tgaagagggc aattgttcac tggaagaact caagcaacaa ctgcaagctc ttcaaaagcc
     4441 gatgtctact tcttcgtttc tttcactcca acagtgtgaa gcaaaagtga aagaatggct
     4501 tcaactaaaa gacctctcta gtcgacaatt attatgtgac tatgtttgtc ggattgaggt
     4561 aacaaaggat aaaacagttt gtgggattta cttccgacaa ctgacacaaa atattttaga
     4621 aaggaacgaa tgatctatgg aaatgataca aaccaaacga gtcgcattgt atattcgcgt
     4681 gagtacacaa gaacaagctt tagaaggaca tagtattcaa gcacaagaac aaaatggacg
     4741 acagtttgcc gaacggatgg gctatgaagt ggtagaagtt tatgccgatg aaggaatcag
     4801 tgggaaatcc acaaaaaatc ggttcgccta tcaacgaatg atgaacgatg ctcggaacca
     4861 tcgttttgat ctactagtga tttggaaatt gacccgtctc ggacgcaata tgttagatgt
     4921 cttacaaaca gtcgaggaat tattgcgata tgacgtagga ttacattcga tctcggaaca
     4981 atttgacgtg accacttcga caggtaaatt gatgttacaa ctccttggga gtttcggtga
     5041 atttgaacgc aaccaaattg cggagaatgt tcaaatgtca atgcaaagct tagtgaaaga
     5101 taaaaagcgt tatgcaggag gccgacgttt aggctatgtc agtggtattg acacgaatgg
     5161 tcaaaaacaa ttggtcattg aaccagaaga ggcgaaaatt gttcgtctga tctatgcaaa
     5221 atatttaggc ggagcagggt atcgagcgat tgccaatttt ttgaatcgtc aaggatatca
     5281 aacagtcaaa aagaatgctt tttctaccac ggcagtcaaa gatatcttac gcaataaaat
     5341 ttatgcaggg tatttagaat atgcgcgcta catcaattgg gaaaccaaac gacggaaggg
     5401 gaagaatccg agaccgattt tagtgaaagg aacccatgaa ccgattattt cggaagaaga
     5461 ttatcaaaaa gtacaagaac gccttgcttt agaaggaaaa caacccaaat ggaaccatac
     5521 aggtgaaaat gtattgacag gcttgttacg ttgtccagaa tgtggcgcac cgatggcagc
     5581 gagtaatgtc acaaataccc taaaggatgg cacaaagaaa cggattcgat attattcttg
     5641 ttcagtcttt cggaataaag gagcaagtgt gtgtcatgcc aattcgattc gtgccgaaca
     5701 agcggaaaca tttgtcgccg accgattaaa ggagatcgtt cagctaccag aagttcttcc
     5761 ccgattggtg gcagcgttaa atgaagaaat agtgcgacaa agccaaccat tggaacaaga
     5821 actcgtggtg ttactggaac gaaaggaaga attgaagact aaaattgaaa aatgggaagc
     5881 tgctttggaa gatagtcctg aattgtttcc gatgttaaaa gaccgcttag acgaattgac
     5941 tgaaaaacga cgacagttgc acattcgaga aaatgaaatc ttagggatct ttcaacaagg
     6001 agagccgatt caagtcaaag atgtccaacg gatattgact agtttggatc gttttcttgc
     6061 ccagagtgag aagaagcaaa tcaaagcctt gtatcgtacc tttatagaga aaataacctt
     6121 tgatcctaac cataaggaga tgttggaaat tacgatgaaa ttcacgcatg ccgtggtaca
     6181 acaattgaat gagcaatatc aaatagccgt atccaaaaca aaggatacgg ctatttttgt
     6241 gttaaaaaca ccctttacgt taacgatcta gtctaggtta tatcaaacaa cgttagaggg
     6301 gcgctaacgc ttctctagcg atttgaaatc gttagagaag tagttaggga ggtccttatc
     6361 ataaataggg atatttatta atgaatattt gaaatataat tgtatataag aattaaaaag
     6421 agtggggata gatatgaaaa agatgggtaa gtgtatttta tgtgaaaaat acactgaact
     6481 tactaaagaa catgtaccac ctaaaaaatc agggaatact ggaggtaaaa agaccagaac
     6541 tggtaattta gatgatttcc ttaagagcga ttttacaaaa ggtgattttc cgaaggggat
     6601 taaaaggaaa cctcaaggaa atgtttatta ta
//
LOCUS       DACDIH010000108         6562 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.109, whole genome shotgun sequence.
ACCESSION   DACDIH010000108 DACDIH010000000
VERSION     DACDIH010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.109"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(151..549)
                     /locus_tag="IV281_002558"
     CDS             complement(151..549)
                     /locus_tag="IV281_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP8781514.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(546..1319)
                     /locus_tag="IV281_002559"
     CDS             complement(546..1319)
                     /locus_tag="IV281_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8781515.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1316..1999)
                     /locus_tag="IV281_002560"
     CDS             complement(1316..1999)
                     /locus_tag="IV281_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8781516.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2166..3053)
                     /locus_tag="IV281_002561"
     CDS             complement(2166..3053)
                     /locus_tag="IV281_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8781517.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3050..3919)
                     /locus_tag="IV281_002562"
     CDS             complement(3050..3919)
                     /locus_tag="IV281_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8781518.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4056..5342)
                     /locus_tag="IV281_002563"
     CDS             complement(4056..5342)
                     /locus_tag="IV281_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8781519.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5615..6562
                     /locus_tag="IV281_002564"
                     /pseudo
     CDS             5615..6562
                     /locus_tag="IV281_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292017.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
BASE COUNT         2051 a         1427 c         1088 g         1996 t
ORIGIN      
        1 catattttga aaagaaagtc tgatgcttag agtagaattt cataaaaatt tactatttca
       61 taagtattca cgagaaaaat gcaaacaaaa aagcggaaat aacgctcatc cgccttcttg
      121 tttgtgttta tttcctcttc aaacgcttgt ctatgattca gacttttctc tatcgttatt
      181 ttcatctaaa agttcttgca ttccgataat ttctcgatat tgatgaatct tagtgatacc
      241 aatgactttg tctacatcaa tactaaaaca aataccatta cctggaatat cgatgttgat
      301 gccttgacca atcgcatcca ttactttatt cgtcaaacta tcgggtacta atgtcaacac
      361 aatatctttt tctggcgaaa tgtccaaacc taaaaatttc cctgaatcat gtacaccaga
      421 acctcttcca tgtaagatcg ttccgcctgc tgcacctgct tcttttgaaa agtcgataac
      481 atcttcgccc attcctccgt caacgatcgt cacaatcatg tgaagattca atgctacaag
      541 atctttcatt tgctttcccc caatttttgc aatctttctt tttcgattct gtctttttca
      601 tcttttcgtt tataaatata tcctagtact aaagtggata atattggtgt caatgcaatc
      661 attgcaacca caccaaaccc gtcaatgatc ggatctcttc cttcaatttg tgcagcgatt
      721 gccacgctca ttgataaaat aaatgtcgaa cacatcgggc ccgttgctac tccaccgtta
      781 tcaaaagcca ttgctaaaaa gatcttgtcc acttttcgtc ctaaaataaa tgctagcccg
      841 tatcctggga tcaagaaata ataaagagag aatccgacga gcaaacgcca catagacaat
      901 cctactgctg cgccgatccc aattgaaatt accatcagca tgattttcga tcggattctc
      961 ccctcgctca attcttcaac ctgtttgatc attacatgaa tcgccggctc agcaaaccct
     1021 actaagaacc ccattaaaaa gccaagggga accagtatat aattgtaagg caatcccgca
     1081 atagcactgc ctaaatgctg tcctaccggt acataagcca tattgactcc gtgcaggaaa
     1141 aggatcaacc caatagtcgt tatcaagaaa cctttcataa tgtcaaagaa acgctttttg
     1201 cgcatacgaa aactaatgat attcattaaa acaaatagga ttacgatagg tgccactgcc
     1261 aaaatgactt ccatggtaac gatttcgatt ccatctatca cttgctgcca gaaactcatt
     1321 ggaagatcac ccccaaaatc atcacggcaa gaatcggccc taacgaagca ataccgacca
     1381 ttccaaaact gtcattttca tttttctttt gtttgatcat actagtcatc cctccggcaa
     1441 gggccaagat aaatggaaca gttactggac cagtagtcac tccccccgaa tcaaaagcaa
     1501 tcggcataaa ttcggaactg gtgaaaaatg aggcgatcaa aacgcctatg tagcctatag
     1561 ccatcaattg ataataggat agcttgaata cgacccgaag cagcgcaaag gctagaaata
     1621 ctccagtgcc gacgctgacg atcccaatca gcaacactct gccaatctct ccgtcagata
     1681 tatcatatac ttgttgtccc aaaacttgca cggaaggttc agcaatcgtg atcacgatcc
     1741 cgatagcaaa tcccaaactg accaaaacgg tcaaactttt ctttttgatc atatatttcc
     1801 ctactagatc tccgacttcc atcatagaat aatctgctcc aaacaaaaag agcgtcattc
     1861 cgatcatcat gattgccgca ccaaccagaa atgaagcgag gtcttctcct tctaacggtg
     1921 caaaaacaaa cgttaaaatg acaatcagga cagtcatcgg caatatagca gcaatcactt
     1981 ctttaaaatt ttttagcatg tatcctcctc ctttactctt taagtttacc ttaaaatgaa
     2041 agagttttcg agcacaaact taaaacgtta tcatttaatt tttctcaaca taaaataaca
     2101 cacgtcccaa aagtatcttt ttacttggaa aaatgacttt gaaggaacgc gcttattgtg
     2161 tttttttatt ttacagagcc gatcattcct tgaacgaatt gcttttgcaa aacaaaaaat
     2221 acgatggcag ttggtaaagt agcgatgact aatccagcca tgatcattcc atagtctggt
     2281 gtataggaag cacccatagc agataaaaca agcggaaccg tccgtttatc aggagattgc
     2341 aaagctacta agggccataa ataattgttc cagctactca tgaatgtgat gattgcagcc
     2401 gcggcatagg tatttttagc cgtcggcata tacactctaa aaaaaatact tagttctttt
     2461 agaccgtcta atcgagcggc ttctactaga tcttttggaa aagatttaac attttgtcga
     2521 aagaagaaga tcaaaaaagc agtgctaaca gatggcagga tgacaaccaa gaaactgttg
     2581 attcccaaaa ccgttccgtt caattgtgaa aacattcgat ataaagggat catcaatgcc
     2641 gcaaaaggaa ccatcatcga tagcagtaga atattgaaaa ttaggtcttt tcgtttactt
     2701 tgataaatct caaatccata tcctgccatt gacgaaatca gcaatgcaaa cacagtggtc
     2761 accaaagcga tcatcgctga attccaaagg gaacgagtaa aattcagctc attggaaaac
     2821 aaattttgaa aattgatgat caagttactg ccaatcttta tttttcccga tgtgatatcc
     2881 acgggtgtat tcgtcattcc caaaaccatc cagacaaaag ggaaaatgga aataaatgat
     2941 aggatgctta atgctgtata ctttaatact gacccaattg tcgaacgaag ttctaatgct
     3001 tgttttttga cgttggggcg ttcgattgtt tctttttcta ccatattttt catcgacttt
     3061 ctcctcctac tttcgtttgg attatcgaca atatcacaat aaaggaaaca ataacaaagg
     3121 aaactgctgc cgcatatcca aaattaggcg tgaatttata actcaaatta tagatgtatt
     3181 gagatagggt ggtcgttgca tttgctggtc ccccgccagt aatattttgt acttcatcaa
     3241 aaagctgcaa tgtcccgatc gtcgaagtaa tggaagtaaa caaaatgatt ggttttaaat
     3301 ttgggatcgt cactttgata aactgctgcc atttagaagc accgtcaata gatgctgctt
     3361 cataaatctc cgaatcaata ttttgcatgc ctgaaaggaa aaaaatcata ttgtatccag
     3421 tccaacgcca agtgatcgaa agaatgatca gaactctcgc ccaaattgga tgagtcaacc
     3481 agccaatcgg tgtgtcaatc ataccaatgc ccaacaacaa gttgtttaca aggccagatt
     3541 gagaaaatag acttttcatg atcagagaat aactgaccat cgaagtgata catggaagaa
     3601 agatagcggt tcgaaacaac cctttatatt tcaacttttt atcatttaaa aggttggaaa
     3661 taaaaagtgc tagaaacaac atgatcggta cttgtacaag taaatacagg aatgtattaa
     3721 aaaaagcttt tttaaaagtt gcatctgtca acatccgctg ataattacct aacccgttaa
     3781 atgtcatatt gacaggggga ccactttgaa aagacgtgat caaagccaaa accatcggaa
     3841 taaaaaccat gattgatatc aaaatgactg gcaagattaa aaaagcatgc ccgtttttga
     3901 ataaatcttt ctgcttcatt cttttccctc caattctatc attgattatc tacatgaaaa
     3961 gacaagaaag acaatatctg taaggcagac gtgatctttt ttgtcttttc gtttgtctga
     4021 tgaaaaagcg aagaaattca cttttttacc accgatcaat tagccaattg tgcttctact
     4081 tgtttttgtg tatctgctag taccttgtct gtctcttctc cattgatgat tcgatgaaga
     4141 gcttcagcta cgactgcttc aattgcatag gtctcatgtc cgtaattcac ttctggtatt
     4201 tcagatgtcc atgttgaaaa atcgtcaaag atcttttgac cactgtaaaa ttcagaagct
     4261 tcttgataag cttcttgttc attcgcgcta agcatcgtag atactagtcc gatttctttc
     4321 gcaagagtgc ccatcagttc ttcattcgaa gcaaacgttg cttctaggaa atctttcgct
     4381 gaatcttctc cagctactcc tttgatcaca taccagccag cgccgccaag gttagaagct
     4441 tgcgcttttt gcagattttc tggaagtgca ggtattggtg caatcttcca ttttccagat
     4501 tggttttctg ctccttggat cgttgaagaa taccaagcgc cagttgggct agaagctact
     4561 gctcctgatt gtatcgcagt tactccagca ttccagtcag aggtttgttc gactagatct
     4621 tctttcaaaa gagtggcaaa caattcaaga ccatacttta aagatgcatt gtcttggatc
     4681 gtgactgtct ccccatcttc agatgtatac cattctcctg cctgttgcat aatcatcctt
     4741 actcgaccaa ggtcagaagg attgacttca gcgattttct ttcccgtctt ttctttgaca
     4801 tctcgcgcta cttgaatata ctcgtcccac ttcagattct ccatatcctc ttctgtataa
     4861 cctgcttctg ccatcagatc cgtacgatag aagtttgctg ttacgcccga atcgaaagga
     4921 acaccgtata ttttatctcc tactttgttt accgcaaatt tatacggtgc aaaatgatct
     4981 tcctttacaa tagcagacaa attagcgaat gcatctggat aagaagtcaa atagccttgt
     5041 atcctataat cttcaatcaa gacgatattg ggcaatcctt ctgtattccc gctcgctaaa
     5101 gcggtgttca gtttctgaac gatatcatcc tgagacatcg taacaacttc tatctctgtt
     5161 tcttcgttct cataaacttt ttttgcttca ttcactgctt tgatattgaa tgtctcatcc
     5221 cacgcccaga cagtgacttt ttcttctttg tctgctgtac tactagcact tccgcatcct
     5281 gttaagaaac ctgatgctgc cgctaaacaa acgactccag caattaactt tctgatgttc
     5341 atttttatcc gctccttttc catccattag ttgtttacgt ttgctacatt catcatacca
     5401 aaatttgaaa acggtttctt tattattcag atacttcaaa aatttctcct aaaataataa
     5461 aaaaatacaa atcaactatg acaaaaggaa taatcttagc atctagacca aagatcgaaa
     5521 tggtgaggtt tctttaaaat aaaaatatcc ttgcattttc tcctagaaat aagtattttt
     5581 aaattacaag actattctta aggcgggtaa aaaaatgcca atacgcgtaa ttcttatcga
     5641 tgatgaacag acgattttag atggaatgaa acagttgatc aattggtctg agcacgggtt
     5701 tcaattaata gggacttttt ccagtccgat gaaagcgctt gatttttctt tggtacatgc
     5761 aatcgatatc gtggtcacag atgtaatgat gcctgaatta aatggaatcg aattatcgcg
     5821 gctattaaaa cagcaacaac ctgaaacaca gatccttatc ctctctagct atgatgaatt
     5881 tgatatggtt aaagattctt ttaaagaagg cgtcgctgat tatcttttaa agcctcgatt
     5941 gagtcctgat ttttttctaa attccttaac tgccgtttca caaaaaatca aaaaaaagcc
     6001 caacaaaagc ccaatcacca atcgttatga gcagatctgc gagaatcttt cacagcagat
     6061 tgctggacaa actcgtccat tgcttgaccc tcttcttttt gaacacacct tgtttttctg
     6121 ttatataccg aaaaacggcc attcagttca aaacaaggct ctaaacaaaa acagtcgatc
     6181 acccattcat tgatggatag cgacgattcg tttactattt atcctgtttc taccttttcg
     6241 gaagaatccg gctatgtaat caatactgat tccaaagata cacttaggaa gtttgttcaa
     6301 aagtttcgtc aagaagtttt tccaggtgat ttttttgttg tttctgattc ttttgcaatc
     6361 caagatatca atgaaatatt ccaagagatc agaaattgca ccaaaggaca acgtttctat
     6421 cataagggaa agaaccttgc ttatcagcat gaacttcttc cgttattaac caaccacgag
     6481 cagcaagcaa gtagttattt aacaaaaatc gttcataaag actacgcttt agctgtcaaa
     6541 gaacttgaga atttcttcac ga
//
LOCUS       DACDIH010000109         6517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.110, whole genome shotgun sequence.
ACCESSION   DACDIH010000109 DACDIH010000000
VERSION     DACDIH010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.110"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            11..1900
                     /locus_tag="IV281_002565"
     CDS             11..1900
                     /locus_tag="IV281_002565"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012579.1,HMM:NF013070.1,HMM:NF019826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8781520.1"
                     /translation="MNQAMVFKEGAIPEYINDDLAYSEIRRYSELLFILANPKKVTTQ
                     EIMAFIGISKNTARNDFSKIKKELPPDYTLIFDPQNGYMIESSMAKLHTLLLEVFNYL
                     FERHATVNRFESLFSEEREFVGFFIHTYEQTFKLKFSDNSLTKLYYLIGLSLSSFKRR
                     TKGQIDFSYDLQELDEFQMIRSCIDQHYQHAEIAIDYEWLTLLFLSFNTISNEFEILD
                     SRIYQVIMEMITLFQSKSAIRISNKEELTNRLMNHLRPAIYRIRYELPFEIDDLAEIV
                     KENHEVSAVLNFVKESIVPLEAFLRKTIPETELYLISVYFCSELLNSSIIETPVRKKA
                     IVVCSNGLIMANVMAKTLKEIFPDIQFVASSSVREMDKFVEDVDLIFSNQPLKSEIPL
                     YIIQPIMNEEEKNRLRNRVLIDFSTNNISKETRNLTEIIKRYSAVQNEESLQTDIERF
                     LINYANSLIYLDRQSKQAQKISFVELLVSEGIILVSTKQLTWQNAMELSCQPLLKEQV
                     INQNYQTILMSQMADEEAYYFLNNRIAIPHSSPENGVIKGGCSLLISVDPIIFPGNRP
                     IHFISPFAIGKKENYLLAINQLLQLSKDEEYQQEILLCTTEDEVRKKIKNFLRRKQYV
                     NNEFS"
     gene            1878..2528
                     /locus_tag="IV281_002566"
     CDS             1878..2528
                     /locus_tag="IV281_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8781521.1"
                     /translation="MSITNFPELTIILRGYSFEEAMFVMEELAAMNTSVGIEVTTNND
                     DFARIITEGNKKFGQQLLIGAGTVLTFSQADQAIAGGAKFLLGPKKFSTPIFSLAKER
                     GVVTVPSAFTPSEIQVMFDEGADIVKVFPAVTVGPTYFKQLAGPFGIRPLMAVGGVTI
                     DNIAAFKKAGVVYFGVGSSMFQKADIQNRNGQAIRKSIQSYLDKLAEAGSEEDESH"
     gene            2515..2967
                     /locus_tag="IV281_002567"
     CDS             2515..2967
                     /locus_tag="IV281_002567"
                     /inference="COORDINATES: protein motif:HMM:NF012579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8781522.1"
                     /translation="MSLIKLEEKNILLNQSFSNQEEYFQKITKKLMTENMVKEGYLES
                     ITKRETTYPTGLYIGERSIAIPHTDYQLSNVTAILVSVLKDSLHFQRMDDPEEMVSVN
                     VIIHLLFDSPDKQPQLLKELMSKVTNQDFLQAIMEAKSKTEVLTAFNQ"
     gene            2984..3289
                     /locus_tag="IV281_002568"
     CDS             2984..3289
                     /locus_tag="IV281_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIB"
                     /protein_id="HAP8781523.1"
                     /translation="MSKKTIIVACASSMITSTMAANKIRKIAKTNGLPEPRILQLKFS
                     EVAGALESNQVDFIVPTGKLQENVTKGIKVIPGTPFVTGVNEEKSESLVLEALKELD"
     gene            3301..4578
                     /locus_tag="IV281_002569"
     CDS             3301..4578
                     /locus_tag="IV281_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010492829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IIABC"
                     /protein_id="HAP8781524.1"
                     /translation="MNIVIDALNWISSLGPMIMMPIIILILGLFFRIKLNTLLKSALT
                     VGIGFAGVNIIINWFISQVGPSVQLMVKNWGIQTSIMDVGWPARAAATWSFPLAAVMV
                     FIILGINVLMLIFKKTNTVMVDFWSYNHYIFVAALVYYATDKNIILAVIFGAIDAIIS
                     FKLADWTTPLVEDYFELEGVNFPTANSVGWAPLAWLLNKLWDRIPGLNKVDARPEKIQ
                     ERFGFVGEPMFMGFVIGLVIGLLAKEPVDKVLIVGMSTSATMVLTPKMMQILMEGLLP
                     FANSVKKFLNVKFPGNNFTMGVDAALTVANSSAIATGIIMVPITLVLAAILPGNKLLP
                     ISDIAYQAMWLSAWPVAFSKGNIIRGILSTTIITACVLLIATSLAGIHTQLALAGGFQ
                     LPAGMTQISTEDAGTHIIGFILMKIAEFISNIF"
     gene            4597..5745
                     /gene="dgoD"
                     /locus_tag="IV281_002570"
     CDS             4597..5745
                     /gene="dgoD"
                     /locus_tag="IV281_002570"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAP8781525.1"
                     /translation="MKISKITVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRFLVGQNPFAIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKALNVPV
                     YELLGGAARDKIKVYSWIGGDRPSEVVEQAQDRVDRGFTAVKMNATEELHYIDSYEKV
                     AEVVERVASIRERFGDTLSIGVDFHGRVHKPMAKVLAKALEPYHPMFLEEVVLPENEE
                     HFSEVANAVAVPLATGERLYTRWQFKSILQQGAIDIIQPDVALCGGILETRKIAAMAE
                     AYDIAVAPHAPYGPIALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEIFQ
                     YKDGFVDLPTKPGLGLEMDEDRVKEIAQEGLVWTNPKWKNYDGTIAEW"
     gene            5757..>6517
                     /locus_tag="IV281_002571"
     CDS             5757..>6517
                     /locus_tag="IV281_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryldialkylphosphatase"
                     /protein_id="HAP8781526.1"
                     /translation="MINTVTGPIEAESLGTTFIHEHLYVIPNELRKYEDYTLDDIYHS
                     IAEVMAFKQAGGKTIVDLTPINYGRNPLALKMISEATGVNVAFVTGFHKEEFQPKWLT
                     TLNDDEIFELLYSEITHGVTSQNLLPGAMKVGTSKNEVTLAEQRILAIAGQVQKKTGI
                     PIITHCDGGTMGLEQINCLTKNGAKASNICLSHVDLTKDFHYLAQICETGASICMDHI
                     GRELANHDADRVQLIKRLVDAGYENQICLSGDMGR"
BASE COUNT         2148 a          979 c         1359 g         2031 t
ORIGIN      
        1 tttgatatac atgaatcaag caatggtttt taaagaaggt gcgattcctg aatatataaa
       61 cgatgatttg gcttatagtg agataagaag atattcggaa ctgttattta ttctagccaa
      121 ccccaaaaaa gtgacaaccc aagaaataat ggcgtttatt ggtatcagta aaaatacggc
      181 tagaaacgac ttttctaaaa taaaaaaaga gttgccacca gactacactt tgatatttga
      241 tccccaaaat ggctatatga ttgaatcaag catggcaaag cttcatacat tgctcttaga
      301 agttttcaat tatttatttg aaaggcatgc aacagttaac cgttttgagt cactcttttc
      361 tgaagaaagg gaatttgttg gtttttttat tcacacctac gaacaaactt ttaaattgaa
      421 attttctgat aattcactaa ccaaacttta ttatttgata ggtttatcac tttctagttt
      481 taaaagaagg acgaaaggac agattgattt tagttatgat ttacaggaat tagatgagtt
      541 tcaaatgatt cgaagctgca ttgatcaaca ttatcaacat gctgagatag ctatagatta
      601 tgaatggttg acgcttctgt ttttatcatt caatacaatt tcaaatgagt ttgaaatttt
      661 agattcaaga atctatcaag taattatgga gatgattacc ttatttcaaa gtaaatcagc
      721 tattcggatt agtaataaag aagagttgac caatcgattg atgaatcatt tacgacctgc
      781 aatctatcga attcgctacg aattaccatt tgaaatagat gatttagctg aaattgttaa
      841 ggaaaaccat gaagttagtg cggtacttaa ttttgttaaa gaatccattg tacctttgga
      901 agcatttttg cgtaagacga ttcctgaaac tgaattatat ttgatttctg tttatttttg
      961 ttcggagcta ttaaattctt ctattatcga aacacctgtt agaaaaaagg caatcgtcgt
     1021 atgtagcaat ggattgatta tggccaatgt aatggcgaaa acattgaaag aaatttttcc
     1081 agatattcag tttgttgcta gttcttcggt cagagagatg gataagtttg tcgaagatgt
     1141 tgatttgatt tttagcaatc aaccattgaa aagtgaaatt ccattatata ttatacaacc
     1201 gattatgaac gaagaagaaa aaaaccgctt gcggaaccgt gtactcattg atttttctac
     1261 gaataatatc agcaaagaga ctaggaacct tactgaaatt attaagcgtt actctgctgt
     1321 tcaaaatgaa gaaagtctac aaactgatat tgagagattt ttaattaatt atgcaaattc
     1381 actaatatat ctagatcgtc aaagtaaaca ggcacaaaag atatcttttg tggaattgct
     1441 tgtttctgaa gggattattc ttgtatctac gaaacaactt acttggcaga acgcgatgga
     1501 attgtcttgt cagcctttat taaaggagca ggtaattaat cagaactatc agactatttt
     1561 aatgagtcaa atggcggatg aagaggctta ttattttctt aataatcgta ttgctattcc
     1621 acattcatcg ccagaaaatg gggtaattaa gggcggttgc tcgttactta tatcagttga
     1681 tccaattatt tttcctggaa ataggcctat ccattttatt tccccttttg caattggaaa
     1741 gaaggaaaat tatttacttg cgattaacca attactacaa ctttctaagg acgaggaata
     1801 tcagcaagaa atacttcttt gcacgacgga agatgaagtg agaaaaaaaa taaaaaattt
     1861 tttaaggagg aagcaatatg tcaataacga attttcctga attaaccatc attttaagag
     1921 gatatagttt tgaagaggcg atgtttgtca tggaagaatt ggctgcgatg aacacttcgg
     1981 taggaattga agtaacaacc aataatgatg actttgcacg tatcatcaca gaaggcaaca
     2041 aaaaatttgg gcaacaatta ttgattggtg cagggacagt tttaacattt tcacaagcag
     2101 atcaagcaat tgctggtggt gcgaaatttt tattaggacc aaaaaaattt tcaacaccaa
     2161 ttttttcgtt ggctaaagaa cgtggggtgg taactgtgcc atcagctttt acgccatcgg
     2221 aaattcaagt tatgtttgat gaaggtgccg atattgtaaa ggtttttcca gcagtcactg
     2281 tagggccaac ctactttaaa caactggccg gtccttttgg tatacggcct ttaatggcag
     2341 ttggaggagt gacgattgat aatattgcgg cttttaaaaa agcaggagtt gtgtactttg
     2401 gggtaggatc aagcatgttt caaaaagctg atatacaaaa ccgcaatggg caagctattc
     2461 gaaaatcaat tcaatcatat ttagataaac tagctgaagc aggaagtgaa gaagatgagt
     2521 ctcattaaat tagaagaaaa aaatattttg ttaaatcaaa gtttttcaaa ccaagaagaa
     2581 tactttcaaa agattacaaa gaaactgatg acggaaaaca tggtaaagga gggatatctc
     2641 gagtcaatca ctaagagaga aacaacttat ccaacggggc tatatattgg ggaacgctca
     2701 atcgcaattc cacatacaga ttatcagtta tcaaatgtta ccgctatttt agtatccgtt
     2761 cttaaagata gtttgcattt tcagcgaatg gatgatcccg aagaaatggt ttcagtgaat
     2821 gttattattc accttttatt cgatagtccc gataagcaac cgcaattatt gaaggagttg
     2881 atgagtaaag ttaccaatca ggatttctta caagcaatta tggaagcaaa gagtaaaaca
     2941 gaagttttaa ctgcatttaa tcaataaatt ggagggaaat acaatgagta aaaaaacaat
     3001 tatcgtcgca tgtgcatcat caatgattac atctacaatg gcagctaata aaattcggaa
     3061 aatagcaaaa acaaatggat taccagaacc tcgaattttg caactaaaat tttcagaagt
     3121 tgcaggtgct ttagaatcaa atcaagttga ctttatcgtt cctacaggga aattacaaga
     3181 aaatgtaacc aaaggaatta aagttatacc tggaacacca tttgttactg gagtgaatga
     3241 agaaaaatct gaatctttgg tgttagaagc attgaaagag cttgactagg agggtgaaaa
     3301 atgaatattg tcattgatgc gttaaactgg atctcaagtt tgggtccgat gattatgatg
     3361 cctatcatta tcttgatttt aggattattt tttagaatta agctaaatac attactaaaa
     3421 tcagcgttga ctgtggggat tggttttgcg ggtgtaaata ttattattaa ttggtttatt
     3481 tcacaggtag gtccatctgt tcagttaatg gtcaaaaatt ggggtattca aacaagtatt
     3541 atggatgtcg gctggccagc tcgtgcagca gccacttggt cttttcctct agcagcagtt
     3601 atggtattta ttattttggg tattaatgtg ttgatgctga tttttaaaaa gaccaataca
     3661 gtaatggtcg atttctggag ttataatcac tatatttttg tagctgcttt ggtttattat
     3721 gcaacggata aaaatattat attggcggta atcttcgggg cgattgatgc aattattagt
     3781 tttaaactcg ctgattggac aacgccgtta gtagaggatt actttgaact agaaggggtt
     3841 aatttcccga ctgcgaactc agttggttgg gcacccttgg cttggctact taacaaattg
     3901 tgggaccgca ttcctggatt aaataaggta gatgctcgtc ctgaaaaaat tcaagagcgg
     3961 tttggttttg ttggtgagcc tatgtttatg ggatttgtca tcggcttggt tattgggtta
     4021 ttagcaaaag aacctgttga taaggtattg atagtgggaa tgagtacttc tgcaacaatg
     4081 gttctaacac ctaaaatgat gcaaatttta atggaaggat tattaccctt tgctaattct
     4141 gttaagaaat tcttgaatgt aaaatttcca ggaaacaatt ttacaatggg tgtggatgct
     4201 gcgttgacag ttgctaattc aagtgccatt gctacgggta ttatcatggt tccgattacc
     4261 ttagtgttgg ctgctatttt accaggtaac aaactcttac ctatttcaga tattgcctac
     4321 caagcaatgt ggctatctgc atggccggtc gccttttcaa aaggaaatat tattcgcgga
     4381 atactttcca ccacgattat aacagcttgt gtgcttttaa ttgctactag tttagccggc
     4441 atacatacac aattagcttt ggctggggga ttccaattac cagcaggaat gacacaaata
     4501 tctaccgaag atgcaggtac acacattatt ggtttcattt taatgaagat tgctgagttt
     4561 atttcaaata ttttctaata ttcaggaggg catacagtga aaatttcaaa aattacagtc
     4621 tataaagtaa aaccaagatg gatttttgtc aaaatatcta cagatgaagg tattgaaggc
     4681 tggggtgaaa tgatttctgg aactaagact gaaacagttg tggcaggagc ttatgagata
     4741 ggaaatcgat tcttagttgg tcaaaaccca tttgcaattg aacgtctgtt tcaggaaatg
     4801 catcgttcat ttttccgagg tggtcctatt aatggaacga ttgtctctgg acttgaaatg
     4861 gctttatggg atattaaagg gaaagcctta aatgttccag tttatgaatt actaggtgga
     4921 gcagcccgtg ataaaatcaa agtatattct tggattggtg gagatcgtcc ttctgaagta
     4981 gtagagcagg cacaagatcg agtagatcgt gggtttaccg ccgtgaaaat gaacgcgaca
     5041 gaagaattac actacattga ttcttatgaa aaagtagctg aagtagtgga acgtgttgct
     5101 tcaattagag aacgttttgg agatacattg agcattggtg tcgattttca tggtcgtgtt
     5161 cacaaaccaa tggctaaggt tttagcaaaa gcactggaac cctatcatcc aatgttctta
     5221 gaagaagtgg ttttgccgga gaatgaagaa catttcagtg aagtagccaa tgcagttgct
     5281 gtaccactag caacgggtga acgtttgtat acaagatggc aatttaaatc aattcttcaa
     5341 cagggagcga ttgatatcat tcaaccagat gtagctttat gtggagggat tttagaaaca
     5401 agaaaaattg ctgcaatggc tgaagcttat gatatagcag ttgcccccca tgcaccatat
     5461 ggaccgattg cgttagcagc tacattacaa gttgattctt gtacacctaa tgtgtttatt
     5521 caagaacaaa gcttaggtat tcattacaac aaagggtttg atttgttaga ttttgtaaag
     5581 aataaagaga tattccaata caaagatggc ttcgtagatc ttccgacaaa accaggttta
     5641 gggttagaaa tggacgaaga tcgagtgaaa gaaattgcac aagaaggttt agtttggacc
     5701 aatcctaagt ggaaaaatta tgatggtaca atcgctgaat ggtaggagat gagtgcatga
     5761 tcaatacggt tacaggccca atcgaagctg agtctttggg tacaacattt attcatgaac
     5821 atttatatgt cattcctaat gagttaagaa agtatgaaga ttataccttg gatgatattt
     5881 atcatagtat tgcggaagtt atggctttta aacaagctgg tggaaaaact atcgttgatt
     5941 tgacaccaat taattatgga cgaaatccgt tagctttgaa aatgatatct gaagctaccg
     6001 gagtgaatgt agcatttgtt acgggattcc ataaggaaga gtttcagcca aaatggttga
     6061 caacgctcaa tgatgatgag atctttgagt tgctttattc cgaaataact cacggagtca
     6121 cttcacaaaa ccttttgcca ggagcaatga aagtaggtac gagtaaaaat gaggttacat
     6181 tggctgaaca acgaatacta gccatagccg gtcaagtaca aaaaaaaaca gggattccca
     6241 ttattacgca ttgtgatggg gggacgatgg gactggagca aattaattgt ttaacaaaaa
     6301 acggtgcgaa agcaagtaat atttgtttga gtcatgtaga tttaactaaa gattttcact
     6361 atttagctca aatctgtgaa acgggagcaa gtatttgtat ggatcatatt ggccgagagt
     6421 tggcgaatca tgatgcagac cgcgtccaac taattaaacg actggttgat gcgggttatg
     6481 aaaatcagat ttgtctttca ggtgatatgg gacgaaa
//
LOCUS       DACDIH010000110         6305 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDIH010000110 DACDIH010000000
VERSION     DACDIH010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.111"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            157..351
                     /locus_tag="IV281_002572"
     CDS             157..351
                     /locus_tag="IV281_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781527.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            620..919
                     /locus_tag="IV281_002573"
     CDS             620..919
                     /locus_tag="IV281_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781528.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1016..1450)
                     /locus_tag="IV281_002574"
     CDS             complement(1016..1450)
                     /locus_tag="IV281_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8781529.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1682..2392)
                     /locus_tag="IV281_002575"
     CDS             complement(1682..2392)
                     /locus_tag="IV281_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8781530.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2382..3242)
                     /locus_tag="IV281_002576"
     CDS             complement(2382..3242)
                     /locus_tag="IV281_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP8781531.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3247..3885)
                     /locus_tag="IV281_002577"
     CDS             complement(3247..3885)
                     /locus_tag="IV281_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP8781532.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3900..4199)
                     /locus_tag="IV281_002578"
     CDS             complement(3900..4199)
                     /locus_tag="IV281_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8781533.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4242..5726)
                     /locus_tag="IV281_002579"
     CDS             complement(4242..5726)
                     /locus_tag="IV281_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8781534.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5746..6207)
                     /locus_tag="IV281_002580"
     CDS             complement(5746..6207)
                     /locus_tag="IV281_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8781535.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
BASE COUNT         1957 a         1318 c          994 g         2036 t
ORIGIN      
        1 gccttttatt atatatatta ttcaaattag ttatatcgag tgaaaaaaac tttcaaattg
       61 attcttttta tttatcataa agatatagat acataatcat tttcacttac atatatttga
      121 atattaacta aaaacagcaa gaaagaagag gatcaaatgt attatgtaga agtaaaaaca
      181 aaaggcgtca aaaacaaaca atatgtgaaa ggtatcagta acgaatatcc tttacttggt
      241 tcatggaaag aagctgctcc tttttccaaa ccatgtgcca taaaaataaa gaacgagcta
      301 gaaaaagaac tgacttgtgg taaagctgtt gtagatatca tcgaaaagta aatccaatgt
      361 atagaaagga agatcacaca tgaaaagcga cgtacaaatt gctaagatta tagaattaga
      421 agaagaagac gcagtgttga caattgaaaa aaaataaatg tttgtatacc atatgattat
      481 ttcaatatgt atcctggaat tggagatctc gtagagctat atcaagatga atcaaatata
      541 cttgtcattc atcatgaaat atagagactt ataaaattgt ttatttttat aatgtatcag
      601 tatagaaagg aaataaaaca tggattatac tgttgaattt gacagtgaac tacttaagca
      661 atttccacaa gaagaacatt tcatgtataa agaagcaatt tcccacaaag tgacagagta
      721 tttggagcat aatttttttc gtataaagcc agtgcgtact cgttcggcaa aaggaatata
      781 cgagatgaaa gtcaaaatta ataaaaaaaa ttatcgcata gcatttgctt taaaaaaaaa
      841 tcatgtgttc attttttata ttagttcgaa tttgcaaaaa attgtatttg ataaagaggt
      901 gagtaaaaaa ataaaatagc tgagcattgg aggatagatt gagtgatact aagtctatcc
      961 tcttgttttt ctaaagaact aagctagaat tttttctttt gtacgttatt atatgttatt
     1021 tgtcattacc aaaaagcacc ttttttcttc tagtatctat gacataagaa ataccaccca
     1081 tttcctttac ttgaacaaat cctactgatt cataaaattt ttttgttttc tcagtatcat
     1141 ctgtcaataa ttcgatttgg actggtccat aactagtgat cttctccaaa gcttcattca
     1201 aaagcacttt gcctattcct tggcgctgat acctcggatg aatgaggata tcttgaataa
     1261 atgcgatcgt cgcaaaatca gtgatccaac gaacgattcc gataatcttc ccttccccat
     1321 tacgctttat cagagattca gattgttcaa atgctctagc taaccttgca gtgtcttttg
     1381 tgtaattcgt ccaaccaacg ctcttatata attcagaaat ctcatcgatc ataagggaat
     1441 caatattcat ttttttcttc ctttcttata caattctccc tctaataata cagttctttg
     1501 actatttaat ctatcacata taactctcct gtaaagattc tctcgattcc ttaaaattcc
     1561 tctttccttt tagaatgcat actttttgat aaataaaaag gttgtgataa attttcttca
     1621 caacctctgt ttctctctat caagtgttat cttttaattt tctaccctta ccctagcatc
     1681 tctattgccc gtagtatgca tactttccat gtttgcgaaa ataatgttta tctagtaaat
     1741 attgcgggat ttcacatata ttggggttga tcagctcgtt ttctttggcc atcagacaaa
     1801 tttcatctaa gatcaaactg ttttctactg ctttcatagg tgaatccccc catgtgaacg
     1861 gtccatgtcc atagacaagt actccaggga cttcgtttgg atctaatttt ctttccttaa
     1921 acgtctcgac aatcacattc cctgtatgaa cttcgtaagc ttctcttact tcttctttcg
     1981 ttaattggcg tgtacaagga accttaccat aaaaagcatc agcatgagtc gtgccataag
     2041 ctggcaaatc gcgtcctgct tgcgcccaca tgacggagtg cgtggaatga gtatgagtaa
     2101 tagcagttat atcttcaaaa gtttgataca aaacaacatg tgtggggaga tctgaagatg
     2161 gttttaagct atcttcttct attatatttc ccgacaaatc tgttaccacc atttgatctg
     2221 cttgcatgca ttcatatctg acaccgcttg gcttaatgac gatgactcct agctcacggt
     2281 ccacttcact cacattgccc caagtcaatt tgactaatcc gtatcgcggc aatgccagat
     2341 ttgcttcaag gacacgttgc ttcattgctg caattttttc ttcacaagac atcatagcca
     2401 gcctccttca atttagaact taagtagtct tttgcctgct ggatttctct tcgaaagtct
     2461 tggctttttt cactccacat ttcaatcaga aatgttccat cataatctag acgtttcaaa
     2521 tgtcttaata atccaagaaa atcaacacaa ccgctaccaa aaggaacatc acgaaactgg
     2581 cctgaatatt cttttgttac agggtaagta tcttttaagt ggatagcagt gatataatcg
     2641 atccctttct caagttctcg agccggatta ttttccggcc atgcggacaa attgcctagg
     2701 tctggatata cttgtagaaa aggtgaatga atctgttctt tgatttctaa aaattttgaa
     2761 atggagttca taaatggatc atccatgatt tccacggaaa gaatgatgcc atatttagaa
     2821 gcttccttta cgctttcttt tattccttcg ataaagtctt ctctggaatt cattgatttc
     2881 tcttcataat aaacatcgta tccagctatt tggattactt tgacgcttag attatgcgcc
     2941 aaatggattg cttttttcat tagttgtttc gctgcttttt tcttttctgg atcagcagaa
     3001 ccaaacggaa atcgccgatg cgcacttaaa caaatcgaat ggatacgtac accggagctg
     3061 aacatctctt ctctcagctc agctatcttt tcttcagacc aatctaatcg tgctaaccgc
     3121 tcatccgtct catcaatcga catctcgata aaatcgaagt ccatttcttt tacaagggaa
     3181 aaacgttctt tccatgaaat atctttgggt aacgcttttt cataaatccc aatttgagcc
     3241 atttccttac ccccagatac gaatgatctc atctttgaag ttttgtgcgg ccttcatcgg
     3301 atcatttgct gctgtaatac cacgaccagt gataaaggta tacacatcga tcccttcaaa
     3361 taagtgtaat gtgtgtggat ttaatccacc agttacagaa acattaaagc ctagttcaat
     3421 cagttttttg atcttcgata aatctttttc accccaagtt tcaccagata acagcgcatc
     3481 acggctttga tggtagatta cctgccggat accgatgtta tgccaatcca tggcttgttc
     3541 aaaagtccaa ttgccgtaga gttctacttg caattcacct atttcttttt gtgctgcctc
     3601 catagttgga atcgttgcac aacaaatgac tgtcatccag tctgctcctg cttttgcaac
     3661 attactcgcc actgttcctc cagcgtctgc acatttcgta tcagcgacta gttttttatc
     3721 tgggaacata ctgcgaatac agcgaatcgc tttttgtcct tcctgtaagc aaagaattgt
     3781 gcccacttca ataatatcta ctacatcacc aactttcata acatctccta atgcatgttc
     3841 taagtcattg tgatctaacg caatttgcaa attaggcctg ctcatcttag ttccttcctt
     3901 tacttgtttt tttggtacca ctttgtttca gcaaattttt cactgatttc tttgactgac
     3961 atcacatttt tgataccgat aactgtaact ccttttttct ctgcctgagt aaacatattc
     4021 aaaaaattga ctggcgtaaa aactacatcg tattggccag ctgaactttt tccctctgaa
     4081 atggcacaat gatggatttt ggtaattggt acgtttagtt ctttcattgc tttttccaca
     4141 cttctcatca tcatcaagct tgttcctgat ccatttgcac acgatactaa aattctcatc
     4201 ttaatccctc cattatgagt gttgtctaat cagattttct tttatactct ctgcttttct
     4261 gcatatgtgt catagtcttc tacaatcaaa aaataaccat caggattttt ccgatattgt
     4321 aactgcggaa tagccaataa accaatgaca actacggcaa ttccagcata acctaggact
     4381 ttcatgatta ctgtaaacaa tggccaagct gttgcccagt cgaacatccc gatataacca
     4441 ccgtatgttg acatcccaat ccatgtagcg aataatgctg aaccgcctac ttgtatgatt
     4501 cctgaaagaa acgggaacag gcaagcggct ttgaatccac ctcggttatt ggcataaact
     4561 gctataacgg cattatcaaa gaacaacgga atgaaaccgg ctatgacgat cgttggtgat
     4621 ttcaataaaa tcagtaaacc aatcatcaaa aactgtccca aagcaccaaa caagaaccca
     4681 atcgttaccg catttggcga accaaagcca aaagtagcag ctacgtcaat tcctggaaca
     4741 gctcctggta aaatcgtgtt tgagattcct tggaaagatt gagttagttc atcgacgaat
     4801 gtccgaacac ctaattgcaa gatcgccaga tataccgcaa aatttaaaga agtcgtcata
     4861 atgtagaata agaaactctg cccttcttgc atgaattgcg cttgaatcaa ataatctttg
     4921 cctagtacaa tcagaatgat cccaaagaaa aatagcatga gtaacgaagt agcaaccata
     4981 ttctcattga aaatcgacaa gaatcctggt aatttgatat cttcaatttt tttgtcttgc
     5041 ttgattgatc tatttttctt ttcatcccgc tttttcatcc actcagctaa ttttgcaaat
     5101 ataaagattc cgaacatttg ctgatgtgcc actgcaaatc cggcaccttc tgtcaattcc
     5161 tgcgtgattc ctactgttaa gttagaagct actgcccaat aacaacctaa aatcagtccc
     5221 ataaaaatca atacttgtat cctacctaga tcaggaaagc aaaacagtaa aatccaaaaa
     5281 gcggtagctg cttgttgaac ctgaacgttt ccggtagtga aaacggaacg cagtttcgta
     5341 tatttctgaa aacggactaa taagatattt aaaacaaatg caatcagtaa taaaatcatc
     5401 acatctccaa aggtacggcc aaatgtctcc attaatccag catccacagc attttgtcca
     5461 aaataaggat ctgtaaccat ggcatccaaa ttgaaccgtt cctttagtcc aaccaagatt
     5521 ggccgaaaat tattgaccaa accaccagag ccaacagtca aaataaagta accgaccgtt
     5581 gcctttaaga atccagcgat gcattcatga agcgggcgtt tcagcaaaat atagcctaat
     5641 aacacgataa aaccaatcag gtatgctggc tgcgtcaaga tatttgttgc aaaatacgtc
     5701 caaatgctta acaaaaattc ccccatcttt tttctcctct atcaattaat tatattctgc
     5761 catcacaact tggtaatcat cgatggagtt cgtagagatg agacgctcaa ccaatccatc
     5821 tgtcattaat aattcggata atttttggat attttctaga tgttcttctg gatcttttgc
     5881 tgcgagtgta aaaaacaaac tagccgtttt ctcctcattc tccccttcac tttcaaaacg
     5941 gattggctgt ttcaacttgg taaatgaaat agctgttcca aaaacaccat cacttttttc
     6001 tgaagaatgc ggcattgcta cttgaggtgc gatcacaata taaggaccgt atttttctac
     6061 acaatggatg atctcctcca catacgtttc atcaataatt tccttatcga tcaatacaag
     6121 acagctttcg gcgatagctt cacgccaatc cgtgatttct ttatctgggt aatgaatcaa
     6181 ttgatggtca aaaaagtatt tcagcaagaa actccccttc tttcttataa aaacgacgga
     6241 aacggtgtat cattttcaat cgagaagaca tcttcaaatc ctcgataaaa attaaactca
     6301 agatc
//
LOCUS       DACDIH010000111         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.112, whole genome shotgun sequence.
ACCESSION   DACDIH010000111 DACDIH010000000
VERSION     DACDIH010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.112"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            725..811
                     /locus_tag="IV281_002581"
     CDS             725..811
                     /locus_tag="IV281_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP8781536.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IV281_002582"
     CDS             complement(932..1621)
                     /locus_tag="IV281_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781537.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="IV281_002583"
     CDS             complement(1628..1906)
                     /locus_tag="IV281_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781538.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="IV281_002584"
     CDS             complement(1866..2780)
                     /locus_tag="IV281_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8781539.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IV281_002585"
     CDS             complement(2762..3142)
                     /locus_tag="IV281_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP8781540.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IV281_002586"
     CDS             complement(3592..3879)
                     /locus_tag="IV281_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8781541.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IV281_002587"
     CDS             complement(3899..4123)
                     /locus_tag="IV281_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP8781542.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IV281_002588"
     CDS             complement(4763..5296)
                     /locus_tag="IV281_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8781543.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IV281_002589"
     CDS             complement(5317..6159)
                     /locus_tag="IV281_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP8781544.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACDIH010000112         6011 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.113, whole genome shotgun sequence.
ACCESSION   DACDIH010000112 DACDIH010000000
VERSION     DACDIH010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.113"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(51..1517)
                     /locus_tag="IV281_002590"
     CDS             complement(51..1517)
                     /locus_tag="IV281_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP8781545.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1904..2500
                     /locus_tag="IV281_002591"
     CDS             1904..2500
                     /locus_tag="IV281_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8781546.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2558..3040)
                     /locus_tag="IV281_002592"
     CDS             complement(2558..3040)
                     /locus_tag="IV281_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781547.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3146..3343)
                     /locus_tag="IV281_002593"
     CDS             complement(3146..3343)
                     /locus_tag="IV281_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781548.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3638..4396
                     /locus_tag="IV281_002594"
     CDS             3638..4396
                     /locus_tag="IV281_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP8781549.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4456..4884
                     /locus_tag="IV281_002595"
     CDS             4456..4884
                     /locus_tag="IV281_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781550.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4930..5002
                     /locus_tag="IV281_002596"
     tRNA            4930..5002
                     /locus_tag="IV281_002596"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4963..4965,aa:Gln,seq:ctg)
     gene            complement(5152..5523)
                     /locus_tag="IV281_002597"
     CDS             complement(5152..5523)
                     /locus_tag="IV281_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8781551.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5738..5923
                     /locus_tag="IV281_002598"
     CDS             5738..5923
                     /locus_tag="IV281_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781552.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
BASE COUNT         2033 a         1164 c          992 g         1822 t
ORIGIN      
        1 aaaaacagga actgctgcaa ataagcaatt cctgttttta tgttcattga ttaatgaata
       61 gagcccatta gttttttgat tggttctttc acagtaaata gcaagatacc accgacgatt
      121 gctactgcac cgataatacc gaagtaagca gattctgttc ccggtgtgta gaagcgtgca
      181 atttgtgcat tgattgcttg tgaagaagca tcagctaaga accaaatagc gattgtttgt
      241 gattcaaatg ctcgtggtgc taattttgtt gtcacagaca aaccaactgg tgaaagacac
      301 aattcagcaa taaccacgat gaagaaactt cccaacaacc acaatggact tacttttcca
      361 cttgtaccat aaagcatacc cggcaacatc aatagcaagt acgataatcc tgcaaataaa
      421 agtccaagag agaatttcac tactgtagat ggttgacgtt caccaagttt cgtccacatc
      481 gttacgaata ctggtgtcag aataaagatg aaaattggat tcaatgattg ataccagctt
      541 gcttggatcg ttacaccaaa tagcgtagat tgtgtacgtt cactagcaaa taaagctaag
      601 atagatgaac cttgttcttg taatgaccag aaaatgattg ctgcaaggaa taatgggata
      661 taagcccata ctcttggttt ttcttcagca gttacatctt ttgaagtcag cattttgata
      721 aagtaataca acggtaagat aatccccaat acactgattg tattgacaaa aaagtcaatt
      781 gtcagatgac cagttagttg tgccccgccg aatactgcag cgataacaac aagtgcgatg
      841 atcaagttac ggatgaatcc tttacgttct tcagttgtca aaggattcgg cggtgttgta
      901 cccactccgt ctaatgtctt gcgtccttgg atgatgtatt gtaataatcc aaagaacatc
      961 ccaatagcag ctaaagagaa ccctaaatga tagttatatt cttgaccgat tgtaccaacg
     1021 attagtggtg ctaataatgc ccctaagttg acacccatgt aaaaaatcga aaatcctgca
     1081 tctcttcgca agtcagtctt tgaataaaga tgtccgacca tgccagaaac atttggtttc
     1141 aaaaaggcgg ttccgataat aatcaatgcc atcgaaacaa acaatgtact tacaccaaat
     1201 ggtaaagcca agacgatgtg gccaagcata atgaaaactc caccgaagaa tacagtgcgt
     1261 tttgctccta acactcggtc agatacccat ccgccgacga tactagacat gtagatcaaa
     1321 gatccgtaga tcgacatgat agctaatgct gttgctcgtg gtaatccaag tcccccgttg
     1381 gcaactgtat cgtacatata atagataagt aatgcacgca taccgtaata actaaatcgt
     1441 tcccacatct cagtgaagaa caaagttgac aatcccttcg gctgcccgaa gaagcccttg
     1501 tcttgctgtt tttccataat ctatacccca attctttaaa ttttcagaaa atattataca
     1561 tctttaccga aaaatttaac taaaaaactt atttaagaaa tataaactaa ttttctataa
     1621 aagcaagtaa tttttaatgg caatatcata aaaaaaagaa gatagagatt taataattat
     1681 atatcagctg tttttttcaa ataagcaaaa attcctaaaa cacaacaatg aaagcgataa
     1741 ctaagaaaat ccttacataa cgatttttct tattaaaatt gttttttgaa cttcgcttat
     1801 tttttgtgag catatctgtt tacttacttt ttgtaaggtg ctatacttca tttcgtaatc
     1861 aattaaaaaa atgcaaaacg caaatcaaag gagaaatttt attatgacaa cttttactga
     1921 cactttgaaa aaccgccgtt caatctatca tctaggacgt aatgtttcac tttctaatga
     1981 agaattaaca actttgatca aagaagcaat taaagaaagc ccaactgctt tcaatgctca
     2041 atcaacacgc gcagttatcc ttttcggaga tgcacatgaa aaactttggg aaatcactga
     2101 agaagcactt cgcccattga ctccagcaga agctttccca aatacacaaa acaaattagc
     2161 tggtttcaaa aatggttatg gtacagtatt attcttcaaa gatactgacg tggtaaaagg
     2221 tctacaagaa caatttgaat tatatgcaga caacttccca gattggtcag aacaatcaaa
     2281 cggaatcgca acagctaata cttgggtagc attagttgat aaaggattgg gcgcaaactt
     2341 gcaacattac aacccagtta ttgacgaagc agttgcaaaa gaatggaata tcccttcaaa
     2401 ctggaaacta cgttcgcaat tagtattcgg ttcaccagaa acaccggctg gggaaaaaga
     2461 atacatgaac gatgccgatc gcttccgcgt atttggataa ttttatttga aataaaaaac
     2521 agaggttgtg acaaacatct tgtcgcaacc tctgttttta ttctggatca atcataatat
     2581 caatctctgc ctcatcgttt aagcgttggg acaaaaatgg aactaattct tctaatgatg
     2641 cagaaaattt cagcatcggt cctcgccatc cgtttcggaa aaaattcatt tttacgattt
     2701 tagaaccttt tggctgatat aatacaattt tattgatctc agaaaaagga acaccttttt
     2761 tgtcaaagct attcaaaaca agtccttctt ctgtgattcc tctactgtcc aacaaaaaac
     2821 taataaaaat cattgctgac aagatccctt ttgccaattc atctgctgta ggatgactga
     2881 tgagtgtata ccaaatgact agtgacacta agaagggtat caccaagtag acccatcgat
     2941 ttcgcacagt ccgataaata atttcttttc gaaccagcca aagaaataaa aacatcacaa
     3001 caacgaataa aaacaataga accgtaaaga atgttaccat gacaactcgc ctcgcttttt
     3061 cttctccaga aaataggaaa agaatactga tttaccttct caaagaaatt ggacttgcag
     3121 cacgccgcac gtccaattgt tttctttatt caaatgtatc atattttgca acaaccatgc
     3181 ttgctttttc atctaataat tcttggtcct ctgcagcaca ttgggcaact ttgaccacgg
     3241 cgcagtttgt catttttgat acaacttcac caatgaccgt ttttgtaaaa ccaattggtt
     3301 tgcatgcata ttgttctcct aaattaatca tttcttccgt catcaaaatc ctcctaaact
     3361 gctggaactt ttctccacat taccactgct atttctttgg agatttaact cattgatttt
     3421 tatttacagt taaaaatcaa ccttacaggc attatagcgt actgttttcg ttttcacaat
     3481 taaaacagct gttgtattta gaagaatttt tttcaaaaaa ttaaaacata ttccttaaaa
     3541 actaattatt tcctacatat taaaatataa tacttccatt tccaataaaa atacatttat
     3601 aataggaaag ataaacagct gaaaagggag atttcaaatg aagaaaaaaa cgttcaaaaa
     3661 actattgttt ctagggggca cagctgctct tttattaagc ggtacaagtt attacctaca
     3721 tatccattct ccagaacata caatgaacac tgcacaaaaa agttcaacac aaaaaacgaa
     3781 aaagacaaaa gagaaagcag aagttttact agatgttcca ttagagagcc agtttgattc
     3841 tccttcttta gaaaatggct gtgaagtcac ttcgctttct atgcttttat ctttttatgg
     3901 ttatgaaaca actaaaaacc agttagctga gcaattggat tacgtccctg ttttcaatgc
     3961 agatgggact catggcgatc ctaatgaagg atttgttggt gatatcagtg gcggagactg
     4021 ggctatggtc gtctatgttc ccccagttgc ctcccttgct cagcaaatcg tccaaacgga
     4081 ttatcacact tttccaaaaa ccgatgctca actcgatgat atcaaaaaag cactatctaa
     4141 aggaaatcct gtctggactt ctgtgactat caattttgaa gtcccagatg aaactgattt
     4201 tatgacttgg accacaaata atggagaagt aagagtcact cctcttgttc atgcttgtgt
     4261 agtcactgga tatgacaaag aaaatattta tgtcaacgat ccctatggtg taaaaaatcg
     4321 agcagtaccg atcaacgatt tttcggcgat tttcactgca atgggtggac agatgctaac
     4381 tttggagaaa agctagtaat tcaaacaaaa atcccttata ctcaagatac caaacctttg
     4441 aagggagcgt agataatgga aaaattcaca cataaaaaaa tggatccaaa tgaaattccg
     4501 attatttttg tccgtgactg caagggaaat gttcaaggta aagtctctat taatgagtgg
     4561 aacgagcgtc gcagacctgc taccctcaat gaactggaaa tcaaattgta tcgtcaatct
     4621 cttgtttatt acgcagatca agagtacgag aaagccaccg atttattgaa atttctaatt
     4681 gctcgaacag agtatactcg ctttgaatat attgaacgtt tagcaaacat ctatcatatt
     4741 atgaatgaac ctgtcaaaga atatcagtta ttggacaccg tcttgtcagt agcagaactg
     4801 attgcccttc ctgctggatt agaaaagaaa ctagtacgcc gccttttacg agtaaagcaa
     4861 caactatccg atcaagaaaa ataacttgcg tttttttagg aaagtgctat gattattttg
     4921 ttgaataaat gggatgtagc caaactggta aggcacttga ctctgaatca agcaattgta
     4981 ggttcgaacc ctgccatccc aatttgatgt gcttgctgaa aaaatgaacc ccctgtaaaa
     5041 gaacaatctt tttgcagggg gattattgta ttatttttac tactagaatt tagtttacat
     5101 aattaaatta tgaataataa aatcgggcgt attcttaata caaaaactga tttacccaat
     5161 aattgctaat gctttctccg gaactttttc tctaggaact tcctcatacg tttctatgtg
     5221 tgctccttta ttgctaattt tcaaataatg attttctttt aacacagaaa gaccattaaa
     5281 ttcaaccgtt cgttctttcc cattggcatc agcagctttc tgcacatacc ttgctgtacc
     5341 atattcattt acttcctctg gtttttgtgt ctttacatag acctctcctt tttcaactaa
     5401 tggatttagc tgatcaagta taccagggat ttctccataa cttccttcag tataatattt
     5461 cagtccaaat agcaaaatac cactaatcgc cgccagtcca atcaaatatt taatcacttt
     5521 catctttcta cctccttgtt atatattcag tatatgggtc agttaaagaa attccatccg
     5581 tcccaagttg gagatttttc tcctacttta gaagcgagaa tcgagtgttt ttttagtaaa
     5641 aatgatcgtt atttgatatt attgacgtgt ggataaatca gtttttctcg ttttgtccat
     5701 gtaaaaaatc atgttgatag caaaggatgt gttcaaaatg aatcaagaag aattatttca
     5761 aaaagtcaag gaaatgatta agaacggcaa tttcgatggc gcaaaacgat ttatcgaaga
     5821 acataaagaa caattaggac cttataaaga gaaagcccaa aatcttttaa aagatgtaaa
     5881 tatcgacagt gtcaaaaaca aatttaaaaa tctattcaaa taagtaaaac tttcactttt
     5941 gtaaaatcaa aggaggacaa tgctgctgca tcgtcctcct ttggttcttt tctgaatact
     6001 tcatactaaa a
//
LOCUS       DACDIH010000113         5892 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.114, whole genome shotgun sequence.
ACCESSION   DACDIH010000113 DACDIH010000000
VERSION     DACDIH010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.114"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..2849)
                     /locus_tag="IV281_002599"
     CDS             complement(<1..2849)
                     /locus_tag="IV281_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP8781553.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLD"
     gene            complement(2863..4080)
                     /locus_tag="IV281_002600"
     CDS             complement(2863..4080)
                     /locus_tag="IV281_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8781554.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4070..5665)
                     /locus_tag="IV281_002601"
     CDS             complement(4070..5665)
                     /locus_tag="IV281_002601"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP8781555.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT         1626 a         1251 c          972 g         2043 t
ORIGIN      
        1 ctatctaaag tccccacatc aaatgcattt aaaatttgtc ggtaaacttc tttgactcct
       61 tctgttttat tcaatttagc caaagctttt tcaatttctt ctcgtgcatc tgaactattc
      121 gcagcataag ttcctagtaa ttgatcaata taagtagaat caatatcata caacttagtg
      181 gtattttcac tataaaattc gatcgttgaa aaatcttctc cctctttttc tggttcttcc
      241 tccatcaatg aacccttcac ggtctcgtac acgccaattt gttcttccaa aagttgaatt
      301 tgattgttca aagttgaaga ttgcaccatg tcatcattgt aatcatcata agtcactaac
      361 gattcatacg cacgattcaa ttcttgaaat gctttgacgt actcgatacg gatttcaagt
      421 ggcgtatgtt catcaatgga ttccatggat gaaacctctt ttaacttacc atatgcttgc
      481 ttgaatcttt ttttggattc ttggtaactt ggataaatca aacccgattc ttctttagct
      541 tctgaataca gttttgtcgc atcctcgaca ttcttctcca ttgtcgctgg ctttcggaaa
      601 gtaacaatta gtccttttgt tttttcagga tacgtacggt tcgtacgtga aaaggcttgg
      661 atcaatcctg catactctag attacgatcc acaaataggg tttgaatcgt aggggcgtca
      721 aaacctgtca atagacgatc cactacgatt actaaatcga tttgtttccc aaattgtttg
      781 aattccgctc tttttcgtgc caaacgatta ttgatgtctc cgttataacg ctcaatgtct
      841 gctagtgacc aggcagtatc ataatactga ttatattcct caataatttt ttgcatttct
      901 tcttgttggg cacttgaatc tcggctattt tcttctaatg aataggtaat agcgattcgt
      961 gggaaatcag gatcctccat cgttcgtcct tcacggatag gctgattggg aaactctttt
     1021 attaaccaat cagattcttt cgtcatctct ttgatggccc gataatactt tttggccata
     1081 tcaatggaac tcgtggttaa gatcgcagac ttcgttggac ggccatttcg aaaatcaaat
     1141 tttgtatagg cattgtctgg acggaacatt ttatgaatga ctttttggat atgttcctct
     1201 gcttcaaaaa cagtagggtc caaataactt tcttttttga cagactccat ttgatcgatc
     1261 atacgattga tctgttccgg tgaatactct gcatatgttt ctacttcttt taatttccga
     1321 tagattttat tctctaatga agtcggctcc acggtgtttt catgctctac ttgaaatcct
     1381 aaaaccgagc catcttctaa tgcattcttg atggtatagg tgtgcaagac ctctccatac
     1441 tgatcatggg tcgtacgtgc caattgccct ttggcttgtt tccgattctc agggaaaatc
     1501 ggtgtacctg taaagccaaa ccaagtggat ttcggaaaca tttttttgat ttcttccata
     1561 ttttcagcac ttaacgctcg atgacactca tcaactacaa acacgatatg ttgccccatt
     1621 aatttttgga agcgattggt tccttttttc tcttcctgtt tctttgcata tttcaacgcc
     1681 gcatctagtt tttgacgggt tgtaatgatc acaacattcg cattggcatc tgccaacaga
     1741 gtttcactta actctttggc acttcctgtt cccacgatca agctattcgc tttggcatta
     1801 ccagaagaaa ccccagtatt aaactccgaa gcaaatttcg taaactctgt ggtggtctga
     1861 ttatctaaat ctttgcggtc cactaacatg atcgttcgat cgatccccga ttttcgtgct
     1921 aaaagcttag tagatacaaa actagtcaac gtctttcctg aaccagtcgc atgccacaca
     1981 taccctgatt gatgtttatt tgcggcaata aacaaggctt gaatcgcatg cacttgataa
     2041 ggatgtaaca ccatgagggt tttattgtct tgatcttcac tgacaatcgt ataatccgca
     2101 atcaagcgat gggcatctgg gatatttagc acttgtttgc aaaactcata taagttttct
     2161 acctttttat tatctttcgt tcgccaacta aataaaaact taggatgcaa atccttcggt
     2221 aaggcatttg caaaataccg agtcgtttgt tcattggaaa ccacaaacaa ctgtaaggtc
     2281 gaaaagatat tgttccgaaa catgccttct tcagcatatt ttttgatttg attaaacgcc
     2341 tgataaaaac catcttttgc agttacttgt tttagctcga tttggataat cggtaagcca
     2401 ttgatcaata aggtgacgtc aaaacgtcga tcccgcgctt ctatgttact tccacgtttt
     2461 gcaatttgat ggaccacttc ataagtagag attcctccac caatgtcttg attggaataa
     2521 aggataagtg agacagaacc caattgcgga ttttcccgtt caatcgtgat ccgagccatc
     2581 ccattttcac cttttaacca tttcgcagca tcaaatgggg ttttcgtccg tagcaataat
     2641 tcggttttga tcgtttcaaa ctctttatcg gtcaaaggag aatcattcag ttctgcttga
     2701 ttatttgaga tgattttttg acgtaaattg ctccaaagat cttcttctga ttttagatcc
     2761 ggacgatacg tccactgatt atgtccttcc ccgaggacac gaatcaactg ttcttcgact
     2821 tccgcttcct cacggtgact aattttcatt cttcttccct ccctaaacaa acatcttttg
     2881 caagaagcct tttttcgttt ccttcagcaa atctaactta cgttgatgaa gagcgatcgt
     2941 atcatctagc tgcttgaaga aggcaccaat tttttgttgt tcatttatat ccggtggaaa
     3001 ctgaaatgtc ctttgttcta taccaccttt agtcacatgt accattgtcg ttccgttagt
     3061 tgattcttta attttctctt tatcagtcaa tagccaagta tatagatact gtttgtccaa
     3121 ttccttagat aaaaatttaa gtttccaaat atgataatgg tagattacac gttcttcatt
     3181 ccacatttca ggaccgaagc ttgttgccca taaatataag agatctcctt tattagcata
     3241 cttgtcttcc tctaattcta agtttgaata ataccatcta tcattagtat taaaattacc
     3301 tactcttaaa accctatatt ttccagaatc aagtaattct tgttgtttaa aagctctacc
     3361 attcaaaaaa actaccactt cccccaactt ccgctcttcc caatctcccg taaatcctgg
     3421 aaaacgaact tctggcactt ttgctccgtt tttagggaac attttttgta agaatccttt
     3481 tttcgtttcc ttcagcaaat ctaattcgtg ctggtgaagc atgatggtgt gatcaagttg
     3541 tttgaaaaat gaaccgatct tgatctgttc gtcataagtt ggaagtgaga taggtgtctt
     3601 tttaccacta tcaattgtat aggttccgac ggttccaatg cctgcaacgt cacggatata
     3661 cttctgaatt ttgttattta tgaacgagta gtaaaagaag tttccgtcaa ttttatgctc
     3721 attcttgaac caaatgatgt ttccatcctt aaagtaaatt gggttatcat cagtgattaa
     3781 taaggggaca cctattgatc cgacaccagt tacaagcaag tctccttgag aaactttacc
     3841 tgaatttttc gaatattcat tatatttttc ttctgaaatg taaagataat cggatggttc
     3901 ctcatttttt gcagctgaga caatatcacg agcacgtaaa aatctaatac ctgagttagt
     3961 ccaatctgat tgatgaattc gcttcacaga agtaacatcc atcatatctc ctaacttacg
     4021 ctcttcccaa tcttccgtaa aaccaggaaa ccggatttct ggtcgtgtat cattgctcat
     4081 gtttgaacac ctccagcgca ccttgtaacc acgcttcatt ttcaggtgtt gtttgcaggg
     4141 aagagatagc ttctaaaagt tctttttcta gcacttgctg ttcttgtcgt atcttcttga
     4201 tgtcatttcc gacatgaacc atatcaattg gttcttcttc ttcgaaagta tcgacatagc
     4261 gtgggatatt taagttatag tcattttctt tgatttcatc aaaggttgcc aaatgagcgt
     4321 atttctcgac atcttttctt tcagcgtagg tttcgaggat tttttgaatg ttttcttccg
     4381 ataatttatt ctgatttttc cctttaacaa attcacgact agcatcaatg aataatacat
     4441 ctcgagtttg acgattcttc ttcaatacaa tcaccgtcgt tggaatcgat gtaccaaaga
     4501 acaagttcgc aggcataccg atcacagcat aaatactgcc gtcttctaat aatttttgac
     4561 gaatgacccc ttctgcagca ccacggaata aaaccccgtg tggcaagacg atagccatag
     4621 tccctgtttc cttcaaatga tagaaaccat gaagaagaaa cgcaaaatct gctttggatt
     4681 ttggcgctaa tttcccgtag cgattaaacc gagaatcatc taagaaggtc gtatctgctg
     4741 accaattagc cgaataaggt ggattcatca ctactgcatc aaatgtgtaa ggttcatctg
     4801 ttggccagtc cttattcaac gtatccccat tgcggagatt catttcttcg gcatctaccc
     4861 catgtaaaat caagttcatt tttgctagat tgtatgtcgt tgtatttagt tcttgcccat
     4921 gatatttaac gttatctgga tgagttaagt agttacgtac atttaacatc aaggaacctg
     4981 aacccatggt tgggtcaaag acactaaaga atcgtcgttc tttttgatcc aacgtcacga
     5041 tttgtgccat catatcagaa accatatgag gcgtgtaaaa ttctccggct ttcttccctg
     5101 cttctgaagc aaattgactg atcagaaatt catacgcatc cccgatcaca tccccatcgt
     5161 gacctaaaac ttccacgtca ttcaatttct tgataacttc ggtaatcgtc acatttcgtt
     5221 gttgctcatc tgttcctagt ttttttgatt ggagatccac atcatcaaat aacccattaa
     5281 attgattgta ggtagatgcc aattgcacaa aggctttgtt tagatcgttc aattggaacg
     5341 ttgcttgttt tgcttgatcc gctaagacgt tgaaaagata ctctggtgca attgcatatc
     5401 ccaaaatatc aacgattgtg gcaatcagat cttcttttga ttcttcatct gaaagtaatt
     5461 cacgatacaa cattgtttgt ttgcttactg tgtcatattc ctctaaggat tcatctgcta
     5521 aaagaaccac ttgttccaac aatcgatcgg ataaatattt atagaaaatc agtcccaata
     5581 aataattttt atattctgac gcatccattt tactacggag attatctgcc gcactaaata
     5641 atttttgatt taattctgca cccaaaaaga tacactccta tctcttattt aaagttgcta
     5701 tataccctct tattttagca tacccccttt tataaaaagc aaaaaaaaaa agaaagaaac
     5761 ttattcttgc taataaacta cgcgaaagga gtctaacgtt gtttgatata acttagactg
     5821 atcgttaact tattttttat atttctacat agttatcttt agaaccattt cagatacata
     5881 tgaaacaatt ac
//
LOCUS       DACDIH010000114         5832 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDIH010000114 DACDIH010000000
VERSION     DACDIH010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.115"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            14..871
                     /locus_tag="IV281_002602"
     CDS             14..871
                     /locus_tag="IV281_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8781556.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            864..1766
                     /locus_tag="IV281_002603"
     CDS             864..1766
                     /locus_tag="IV281_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8781557.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            1799..2149
                     /locus_tag="IV281_002604"
     CDS             1799..2149
                     /locus_tag="IV281_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8781558.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2183..3121
                     /locus_tag="IV281_002605"
     CDS             2183..3121
                     /locus_tag="IV281_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8781559.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            3199..4092
                     /locus_tag="IV281_002606"
     CDS             3199..4092
                     /locus_tag="IV281_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8781560.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4130..4693
                     /gene="amaP"
                     /locus_tag="IV281_002607"
     CDS             4130..4693
                     /gene="amaP"
                     /locus_tag="IV281_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8781561.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4707..4895
                     /locus_tag="IV281_002608"
     CDS             4707..4895
                     /locus_tag="IV281_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8781562.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            4908..5810
                     /locus_tag="IV281_002609"
     CDS             4908..5810
                     /locus_tag="IV281_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8781563.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
BASE COUNT         2126 a         1018 c         1174 g         1514 t
ORIGIN      
        1 aggagtgatt ttgatggaga agagatacgg cgtagttcct gtggtaactg cttcagacga
       61 aaattatgct ccttacttga gcgtgatgat cgcaactgca ttagaaaatt gcaataaaac
      121 aagacgcata aaattttatg tgattgacga tggtctatca gaatatagta aagaaggatt
      181 ggaagaaacg gtaaataaat attcaagtaa tgcaagtatc caattcctga cagtggaaaa
      241 agatatctac gaagacttcc tagtcagtga ccatatcaca actaccgctt acttaagaat
      301 ttctttacca aacttattag ctaaagagga ttataagaaa gtcctttact tagattcaga
      361 tgtgttagta ctagacgata tcgtaaaact atatgatgag ccgttaaatg gaaaaacaat
      421 cggcgctatt atcgatccag gccaagtgaa agctttggaa agattaggaa tcgattccga
      481 tgacttgtac ttcaattcag gagtgatggt gattgacatt gatcaatgga ataaaaaaga
      541 aattacagaa aaaactatcc attacctcag tgaaaatggc gatcgaatca tctatcacga
      601 ccaagatgct ttgaatgctg tactttatga agattgggaa caacttcatc caaaatggaa
      661 catgcagact tcgctgattt ttgaacgtca tccagcacca aatgaaaaat atgaacgtca
      721 gtataaagaa ggaaatgaaa aaccttcgat cgttcacttt acaggacatg ataaaccttg
      781 gaatacgttg aaagatcatc cgtataccaa tttatactta aaaaaattag cacacagtac
      841 tctaacgaaa gtaggcgaga tcaatgaata aaaaagaaat agcagtagtc gcaagttgta
      901 acacaaaatt cgtaccacat ttagctgcat tatttgtctc agtattagat aattgcaacc
      961 cgtctaaatt tgttcggttc tatgtgattg acgatgatat cgattttgaa agtaaacagc
     1021 ttttacgctt ctcagtgaaa aacgcacgaa tgaactcaga tgtggaattc ttgaaaatca
     1081 ataaagaatt ctttacaaat gtggtaatca gcgatcgtat tccagaaaca gcttattatc
     1141 gtattgcgat tccggaattg ttccgcggca cagaagttga acgtatctta tatatggatt
     1201 gcgacatgat cgctttacaa gatatctcta aattatggcg tcttgacttc ggtgattcaa
     1261 tcgtagcagc tgttgaagac gctgggttcc atcaacgact ggaaaaaatg aaaatttcag
     1321 ctaaatcaat gcgttatttc aactcaggtt tgatgctgat caatgtgaaa aaatggttag
     1381 atgaaaatat tacgcaaaaa gtattagatt tcatcgaaca taatccagaa aaattacgat
     1441 tccatgacca agatgcatta aatgctatct tacatgaccg ttggttacca cttcatccgc
     1501 gttggaatgc acaaggctat atcatggcaa aagcaaaaaa acatccaaca gcagcaggtg
     1561 aacgtgaata cgaagaaact cgcaacaatc cttacattat ccatttttca ggacatgtga
     1621 aaccgtggag caaggatttt gaagggccaa cgaagaaata ttacgaaaaa tatgcaggga
     1681 tgacggcttt ccgttgtgta gctaaattcc ctaaatatcc aaaatatgca aaagttcaac
     1741 caaaaacaga tgtagttgtt aaataataga agacaacaac tgaagagggg gagtaggaat
     1801 ggaaattaca atgtctaaaa atgcagtaga gacgttaatt gaaaaggttg gggaaaatac
     1861 aaaaatcgca ctcgcgctta tcaatgattc cgatccattt ctaagggaca aaggtgcttt
     1921 tgcaaaaggc agcttttttc aaatcattcc ttttgtttcg gaatttggag aatatgcgac
     1981 taaaatcgag catccattat tggacatcta tacatctaaa ttagagcaaa attatttcgg
     2041 taaacgattg aacatggact tcaacaaaca gcttgacagc ttttcattag aaaatgaaat
     2101 cgcagtactc gattataata tcaaattaaa gaactgcttc ttcagctaat agtttcgaga
     2161 aagtccaaaa aggaggtata cattgagata ctactttgtg gatgaagaaa agaatgtaat
     2221 agaaacagat gaaaaaaaat ataattggct cgtacttgat agtgcaaagc ccgaagagat
     2281 tgagcaagca atcgaagaat tcgagcttcc tgatgatatt ttcgtaggaa ccgaatatcc
     2341 ggaagaagtt tctcggatgg agcgccttta tgagacgaaa ctagagcatc cattttcttt
     2401 ggtagtgatt aatctagatc agtcagataa tcgtatcgaa aaaaaattaa cgccaatatc
     2461 ttttgtactt tccgataatc tcttgattac ttgtatcgat agaaaaaccg aatttgtgga
     2521 tcggctgctg acaaagcatg gaaatcattt aaactcgttt gaaaagatca tcacgtatac
     2581 attattagat atttacaccc attacgtgaa agaactaaga gagatgaaaa agcgaattga
     2641 cgctttggat aaagcagctc gtaaaacaac ggaaaatgaa gaactgtata aacaggcaga
     2701 tcttgaaaga gacatcgtat atatcgatca taccttaaga gatcaacggg aaacaatgga
     2761 tcaattatgg aattctgaaa ctttcatcaa acgtttagac gatgaacagc tactctatga
     2821 tgtccatttg agacagcgac aaacagaaaa aatgatcgag atctatcgag atttattaga
     2881 gagtattggt gatctattca acgggatgat ggacaataat ctaaatcata tcatgaaata
     2941 tctagattca gctgcattaa tcatttccgt tccggcgttg ttcgcaggga tttggggaat
     3001 gaatacagga ggattaccgg gtaaaagttc tgctctaggc tttatactcg tagtctttgg
     3061 ctctttgata gcagcagtag ttttaggtta ccatttatac cggaaagatt atacaaatta
     3121 aagaatctat ttaaaacagg tatcaaacaa agaaatttgt tttggcaata caatttaaaa
     3181 actaggagga atacagcaat gacagaacca aaattaatcg acccgcgaaa cttgtaccat
     3241 acggagaaat ttcctaaaca agatcaggat acaccagctt tacagcagaa tatgattcct
     3301 gttcctgatt gtggagaaga atcctatgaa ggcaataatc aactagaaaa ccgccgtgta
     3361 ctaatcactg gtggagattc agggatcggt cgtgcagcag cgattgcatt tgctagagaa
     3421 ggggcagata tcgctcttca attcttccca ggagaagaaa aagatgcaga ggaagtagca
     3481 aaatacgtga aagttgcagg acgtaagatt gtcttactac cttttgatct tcgtgacgaa
     3541 caagcaccta aagaaatcat agataaagcc gtgagtgaac ttggcgggct agatacgatg
     3601 gtactaaacg caggacagca aatttcttgt cagtctattg ctgagttacc acttgagcaa
     3661 gtcaaagaca catttatgat aaacatcatc gctatgtttg ctttagtgaa agaagctgtt
     3721 ccgcatatcc cagcaggagg agcaatcgta acaacaactt ctgttcaagc attcaatcca
     3781 agcgaacatt tattagatta cgcagcaaca aaagcttcaa tcgctaactt tacgatcggt
     3841 ttagcaaaac agcttgcacc taaaggaatc cgtgtaaatg gtgtagcacc tggtccaatc
     3901 tggacaccat tacaactaga cgaaggacaa ccttctgatt cgatccctga atttggtcag
     3961 caaagtttac tagaacgtgc aggtcagcca ggagaattag caccagttta tgttttctta
     4021 gcatcgaata aagcaagtta tgttacagcc caaatttacg gagtaactgg tggagaagcg
     4081 atcaacttat aagaagttga tcgtttcacc cctatgagga ggtgcgaaaa tgaataaagg
     4141 tgtaaaaacg atcggagtga tcggaacatt acttttgcta tctgtgcttt tgtttgcgat
     4201 actattaagt gcaacctatc cattgccttt tcagctagag aagtttcgat tcttttccat
     4261 cactaattca tacgtgcaac aatatgtatt ttggcttgcc gttgcatttg ctattttgac
     4321 aattatcacg ttacttgttt tcctgtttta tccaaaaaca atcggaaact ttctactgaa
     4381 acatggtgat ggggagttga cgttggataa aaaagcaatt gaaggaatgg ttcgttctca
     4441 cttgcatgaa gatgaattca tccattcacc aaaagtaaat gtcaaagcaa cgaaaaacaa
     4501 gatcgccgtc aatgtaaaag gggagttgaa acgaacttca gcactcattg gtaaaacagg
     4561 aacattgatg aaagaaatcg aaaatgaagt acaacacata ttgggaacaa aagagccagt
     4621 gaaagtatcg gtgaaataca caggctttga agaaccagct aaacgaagca atgaccgcaa
     4681 acgagtagaa taggggggga ccaaaaatga aagaactatt ttcgacatac aagctcccta
     4741 tcatctttgg ggtacttgga ttgatattag ccgttttatg gatcaccgtg ggctttttca
     4801 agaccttatt gatcttgatc atggcagcga tcggcgtcgc tatcggtttt tatctgaaac
     4861 aaacgaaaat cttagatcac tattttaaat cttaggagga aacatccatg aataacaata
     4921 acaaaaataa taacaataaa atgaacaacc aaaaaccagt gtctgcacat ccagacccaa
     4981 cgacaccaaa ccaatcaaat ggtgtgaacg atgaaaagaa caaaacagca ccagtatctg
     5041 cacatccaga tccagtagtc cctaacgttc aacaaggagt agaagagaag aaacatgatg
     5101 cagaaaagca tgcacctcat catccaggaa ctccagaatc taaagttcca gaacaacatg
     5161 atgcaatcga tccttctaaa cctgtggaag agcaaaaaca tccttctgac catgcgaaag
     5221 cacaagaaaa acttagttct gaccacacaa acggacaagg aaaacttggt tctgatcata
     5281 caaataacca aggaaagcat gattctgaac aggaagattc aatgcgcggt gagctgaatt
     5341 atgaagataa agttgtgcaa aaaatcatcg gaatcgcaat tgaacaagta gacggtttat
     5401 tatctgcaaa cggtggtttc ttctctaatg tggcaggaaa acttgtgaat acagataatg
     5461 taacggctgg gatcgaaaca gaagtcggta aaaaacaagt agcagttgat atggatgtca
     5521 ttgtggaata tggcaaagat atcgaaaaaa tctttgaaga aatgcaagaa gtaatcggca
     5581 aagaagtaaa aaatatgaca catttagaag tgatcgaagt caacgctaac gtagtggaca
     5641 tcaaaacaaa agaagaattt gaaaaagatc aagaaacggt gcaagacaaa gtaactgacg
     5701 cagcgaaaaa aaccggtgaa tttgcttcga atcaaacaga taaagtaaaa tcagcagctg
     5761 gaagcggcgc acaaaaagta aaagaaaata cagaaccacg ggttcagtaa taaggaagta
     5821 aaacattagg ag
//
LOCUS       DACDIH010000115         5751 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDIH010000115 DACDIH010000000
VERSION     DACDIH010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.116"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..434
                     /locus_tag="IV281_002610"
     CDS             <1..434
                     /locus_tag="IV281_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP8781564.1"
                     /translation="KDYWGESKGFIYPDSNMGGNWKATNPKKEQEAMKLKNTKSNNLL
                     YATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNWKFVENNSGGQNISSVSYETALL
                     EYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVLKKIAL"
     gene            454..1014
                     /locus_tag="IV281_002611"
     CDS             454..1014
                     /locus_tag="IV281_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HAP8781565.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGILSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADE
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            1838..2605
                     /locus_tag="IV281_002612"
     CDS             1838..2605
                     /locus_tag="IV281_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP8781566.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIREKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            2631..3173
                     /locus_tag="IV281_002613"
     CDS             2631..3173
                     /locus_tag="IV281_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781567.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFATYESGMSAYDETGFDGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGYSLDDKTRLRLFDIEGKQIGLNKFCKNGKEFATKDF
                     IKGYRLLLENYGINLEESVK"
     gene            3174..3740
                     /locus_tag="IV281_002614"
     CDS             3174..3740
                     /locus_tag="IV281_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781568.1"
                     /translation="MFQKRKDKFPNLEVKSQKVIDGEMIELFQKNYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            3755..4003
                     /locus_tag="IV281_002615"
     CDS             3755..4003
                     /locus_tag="IV281_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781569.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDDTENLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            4023..4262
                     /locus_tag="IV281_002616"
     CDS             4023..4262
                     /locus_tag="IV281_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781570.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKTQRKGYPDKILKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            4633..4914
                     /locus_tag="IV281_002617"
     CDS             4633..4914
                     /locus_tag="IV281_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP8781571.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFDEA"
     gene            4904..5245
                     /locus_tag="IV281_002618"
     CDS             4904..5245
                     /locus_tag="IV281_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8781572.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIGEWEKAGLKKQSWIDVGSLKSIPKSRENISFKKVGMLSASDTKKL
                     NKFIERLQRKV"
BASE COUNT         2170 a          767 c         1161 g         1653 t
ORIGIN      
        1 aaaaagatta ttggggtgag tcaaaagggt ttatttatcc agattcaaac atgggaggga
       61 actggaaagc tacaaatccc aaaaaagagc aagaagccat gaaattaaag aatacaaaaa
      121 gtaataattt attatatgct acgtgtaaac attttcgaca tgtacgagat acagaattta
      181 cgagttatca tctttcagga atagtaattg acagtttcgt atatgaggct atgggaaatt
      241 ggaaatttgt tgaaaataac agtgggggtc aaaacattag ttctgtatct tatgagacag
      301 ctttattaga atactataac tcacataaag ttatgggcgg tttaaattta tactctcctg
      361 gaagcaatca atttgttaat tctgatagta gtatcatatg tttagaaaaa gtattaaaaa
      421 aaatagcttt ataaagtttt atggaggaac aacatgggag acgaaaaaaa gcgcttggaa
      481 gttcaattac gtgaagcata tggacgagta acgtatactt acaccactca tttaaagcaa
      541 gccgataata ttatttctga caataaaatt ttaaggtgtc ttcaaattgg tttgtcagcc
      601 cttactactg gagggatact tagtaccatt attactgata atagtgagtt aacctttgtt
      661 gctggattat gttcaacaat ttctcttgga ttaaacttat actttaagaa ctttgatctt
      721 gctgaagaag ctaaaaatca tcaaattgtt gccgatgaat tatggttgat cagagaagaa
      781 tacgtaagct tactaacaga tttaaatcgt ttaagtgttg aagatattgt gaaaaaaaga
      841 gatcaattaa tgaacgaaac tcatgaagca tataaaaatg cccctaaaac aaatagtaaa
      901 agctatgaat cagctcagaa agcattaaaa tctgaagaag agcaattttt tacagaagag
      961 gaattggata agatgttacc agagcatctt agaaaaaaga gttctaaata ttgaactctt
     1021 tttttatgct taaaattctc ttgtaatttt tctattcaat caataaataa tctatattta
     1081 agaagaaata tgatgataga aacatctcct aaagccaccg caagccgttc taagcatagc
     1141 ttagcccgac attgctagat atgctcatct caccatgaac gtttcctaaa gccaccgcaa
     1201 gccgtcctag tcattgccta gtccgacatc gctagattag tgcatctcgc cataaaagtt
     1261 ttctaaagcc accgcaaacc gtcctagcca tcgcctagtc cgattttgct tgatcggttc
     1321 catttctgtt tatttagtgg gtcaacaccg taatcttaaa tagccatcgc ctatttaaga
     1381 ttactaggtc tgctttgaag gtaaaaagtc tttcattctt caagactttt aggctttcaa
     1441 gctacgcctg aaagcattga aattcatcgc tctttatttg gttttgccat ttataattcc
     1501 ttacgcccat tatctttttc gcctgtcttg ccttgtcaga agtgtttaga gcttatctca
     1561 tctttggatt aatacaagtg gcgtaaattt ttttctaaac gcaagcgtta cgaaaataat
     1621 ttacttaaac acttgtatta atccggcaga taagaacgca ttctaatcac tcccgacaag
     1681 gcaagacagg cgaaaaagat aaataaatga acgaaaggaa gaaacataaa atgcaaaacc
     1741 aaatagctat gaagaaaaaa acacctccct ctaaaacaat ccaaacaaaa aagaattttg
     1801 aaaaaccaaa aatcaaaaac agaaagaagc gttcaacatg agagatagaa ttttagaagt
     1861 taatcaagaa atggtaaaca agttaacaaa tatcagtggg aactatgaaa agtacgatgg
     1921 tactttactt gataactata tttttcacga tacacaaaat atcagaattg gcagaagtaa
     1981 accgagagcg ttcattctca tcagagaaaa atatttgaat gaatggaata gcacctatga
     2041 aatggttatg actgataact ttgaaaaggt attggatttc agaaatgctt tgttgggttc
     2101 tgatatggga aatagcagtc ttgaatctat tgaagttgaa aacaagaccg agaatgaagg
     2161 tgaaggattg attaagaatg tcttgaaaat cgaacaaaaa tatatcagtg aagatagaag
     2221 tttttataga gtcacaagct cggttggtct agcaaacttt gctactaact taattggaaa
     2281 caatgctagt gaatctttgc tagtgatgtg tctgaataca aagaatgagg tcattgctta
     2341 tagcgaagtt ttcaaaggtg gaattaatca atcagtagcg ataccaagag agatttttca
     2401 actagctttg ctaaacaatt cagcaaaaat cgcagtatca cataatcatc cgagcggata
     2461 tgaatttcca agtgaaaatg acaaaagttt tactacgaat ttgaaaaacg caggaaaaac
     2521 aattggtatt gaattgttag accatatcat cgtaacaagt aaaaacacaa gctattactc
     2581 atttcaagaa aatggttctt tataaaaatt agagaaaaga ggaaaacaag atgggcgaaa
     2641 gaacacaagc gttaattaca gtcaaatatg gtaacagcaa aacgaaagca agcgtgtcga
     2701 ttcattatca gtgggggtat ggtcgatata tgctgatgga cgtattgaat caaggaattt
     2761 atttaatcaa taaagcatac gacttacgag ataaatcaag agaagaatta gcaaaaggaa
     2821 tctttgatag aagtacaggg gttatcaatt ttgccaccta tgaatcaggt atgagtgctt
     2881 atgatgaaac aggatttgat gggcgctatg aaccttatca agaaacgatt gaagatgtga
     2941 tgaagttttc tgataacaat gatggctatg ctcatttaga gatggtgttc gatgaaaatg
     3001 gatatagtct agatgataaa accagattgc gattgtttga tattgagggt aaacagatag
     3061 gactaaacaa gttttgtaag aatggaaaag agtttgctac aaaagatttt attaagggat
     3121 acagattgtt gctagaaaat tatggaataa acctagaaga gagcgtgaaa taaatgtttc
     3181 aaaaaagaaa agacaaattc cctaatttag aagtgaaaag tcaaaaagtg attgatggtg
     3241 aaatgattga actattccaa aaaaattatg gaacgataga aactaccgca agttgcaaat
     3301 actactttca aggaaagaga aacacgttta aaggctttgc cgattctgat gaagaattgg
     3361 caaagaaaat cattgattgg cttgtcagca gtgaacacgt agcaagagaa gataccgtta
     3421 gaacaacgat taatggggaa gtcctaacag gtactagatg tgtttgttgt aatcatgaat
     3481 ttgtagaaga tgaaggttgc taccgaatca atgaatatgg tgattttatt tgccagagtt
     3541 gccacgagat agaatttcca accgaaagaa gttggtttga atacatgcta caagattatg
     3601 agatggaaaa agaaaatggt gaagtagttt tttgtaccaa agaggaattg agtaagagaa
     3661 gcgatgatga aattgaagat tggtgtgtgt ggttagtagg ggcagatgat tgcgttgcat
     3721 attacacaac agcagaatag gagtgtgaat cagcatggaa ggaatggatt ttctagatca
     3781 tgaagatttg gttgattttg gttatacatg gaaaggaatg gtaggtatta gcagaagtct
     3841 tgctaatgcc ttttacgaaa gaaattatgc agtatatgtt ctttatgatg acgatacaga
     3901 aaaccttgtt gacgaggaat ataaattgga tttagagaat gttttatatg gaatagaaaa
     3961 ggaagattta gcaaaatata ttttttcatg gttgggtcaa taagtgaaag gaagatagaa
     4021 acatgacaaa atttaaaagt aagggtatgc aatggtgttc ttattattat gttggaaatg
     4081 atgaagattt attgagaaaa acccaacgaa aaggttatcc agataagata ttgaaacaaa
     4141 taggttatta catgccagaa gagaggatag aaaaagaatt ttgtgaggtt ctttttgata
     4201 gtgaacagct attagttttg ttaacccaaa ataaattgat ttgccaattt gaaaataatt
     4261 aagatgaggt ggtgaaccat gaatgaagat aagatggtat taagtgaata tgctagattg
     4321 agaactatta attctttagc atctcattta agttgttctt tccttacaaa ggtgctaaaa
     4381 ttaatttaca aatgtgaaga ttttgtacaa gagaagcaca aagacaagcc tactgcgaat
     4441 aggcttgtct ttttttcatt cattacgatt ccgtatgtgt gggttagcga caatttgttc
     4501 aattaccgta tgatatagca tcttaatttg attagattac ttgcaatgta gtttattaag
     4561 agtataatat aatcataaat gtggataatt tgagaaataa tgagtgtttt atactcaaaa
     4621 ggagtggatg aaatgaaggt caaaaatcca actgaaaaaa gcagaatcca agtcggaata
     4681 gacaagaatt taaaagagaa cgcagaaatg atattggaag agctaggttt aaatcctaca
     4741 acagcaataa caattcttta caaacaagta gtcgctagag gtgagtttcc agtagagata
     4801 aagctaagtg aagaggagaa gcaaggaatc agattgcaac agctaactaa ggacatgcca
     4861 gtagatgttc tagatacaga tgaaaagtta gaagagtggt tcgatgaagc ctaatgagat
     4921 agtaacggtc tatgttgcat ttacagataa aaatggtggt aaaagaagac ctattttggt
     4981 ggttagtgat aaagaagata gagtagaatt ttttggaatc acaagtcaat atgagaagaa
     5041 atcagaccaa ataaagagag tgtattttcc tattggagaa tgggaaaaag caggtcttaa
     5101 aaagcaatca tggattgacg tagggtcttt aaagtctatt cccaaaagcc gagagaacat
     5161 atcatttaaa aaggttggta tgctatcagc tagtgatacg aaaaagctaa ataagtttat
     5221 cgagagatta caaagaaaag tctaacaagg cttttctttt tttgcttata tgtatattaa
     5281 gatatacgtc tatacaaacg tatttatgta tataaaaata cacgtaaata tatacgtgaa
     5341 tataaacatt aagaaaatat taagaatgat taaatagtgg taattgttaa tatacgaatt
     5401 ataagatata ttttaaatcg aatgtatgta aataaataaa atgactggag gatatttaat
     5461 agtgataaat aacagtaatc cagacggata cagtgagtga agtagcttgc tacgaacgaa
     5521 caagcagaat gaaacggtag tttcattaga gcaagtcgct tgcgacgtag cgttaccgct
     5581 gatgtaactg gcaagttaca tagagagagt ttgcttgcaa acgaacgtta cctacttgaa
     5641 tagaaaacaa aaataacaga ttagtgaatt ttaaatagga gccacaactc aaccaaccgc
     5701 ttgcggtggg tgagtcccac ggaggaaaag acgaacgaac ggtcgcgtag c
//
LOCUS       DACDIH010000116         5699 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDIH010000116 DACDIH010000000
VERSION     DACDIH010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.117"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="IV281_002619"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="IV281_002619"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            203..276
                     /locus_tag="IV281_002620"
     tRNA            203..276
                     /locus_tag="IV281_002620"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Asn,seq:gtt)
     gene            316..405
                     /locus_tag="IV281_002621"
     tRNA            316..405
                     /locus_tag="IV281_002621"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Ser,seq:gga)
     gene            420..491
                     /locus_tag="IV281_002622"
     tRNA            420..491
                     /locus_tag="IV281_002622"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:453..455,aa:Glu,seq:ttc)
     gene            507..579
                     /locus_tag="IV281_002623"
     tRNA            507..579
                     /locus_tag="IV281_002623"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:540..542,aa:Val,seq:tac)
     gene            589..661
                     /locus_tag="IV281_002624"
     tRNA            589..661
                     /locus_tag="IV281_002624"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Asp,seq:gtc)
     gene            697..772
                     /locus_tag="IV281_002625"
     tRNA            697..772
                     /locus_tag="IV281_002625"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:730..732,aa:Phe,seq:gaa)
     gene            774..854
                     /locus_tag="IV281_002626"
     tRNA            774..854
                     /locus_tag="IV281_002626"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:808..810,aa:Tyr,seq:gta)
     gene            861..934
                     /locus_tag="IV281_002627"
     tRNA            861..934
                     /locus_tag="IV281_002627"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:893..895,aa:Trp,seq:cca)
     gene            937..1009
                     /locus_tag="IV281_002628"
     tRNA            937..1009
                     /locus_tag="IV281_002628"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:970..972,aa:His,seq:gtg)
     gene            1021..1092
                     /locus_tag="IV281_002629"
     tRNA            1021..1092
                     /locus_tag="IV281_002629"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1053..1055,aa:Gln,seq:ttg)
     gene            1138..1208
                     /locus_tag="IV281_002630"
     tRNA            1138..1208
                     /locus_tag="IV281_002630"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1170..1172,aa:Cys,seq:gca)
     gene            1229..1313
                     /locus_tag="IV281_002631"
     tRNA            1229..1313
                     /locus_tag="IV281_002631"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Leu,seq:caa)
     gene            1375..1447
                     /locus_tag="IV281_002632"
     tRNA            1375..1447
                     /locus_tag="IV281_002632"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1408..1410,aa:His,seq:gtg)
     gene            1457..1528
                     /locus_tag="IV281_002633"
     tRNA            1457..1528
                     /locus_tag="IV281_002633"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1489..1491,aa:Gln,seq:ttg)
     gene            1546..1630
                     /locus_tag="IV281_002634"
     tRNA            1546..1630
                     /locus_tag="IV281_002634"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1580..1582,aa:Leu,seq:caa)
     gene            1885..2760
                     /locus_tag="IV281_002635"
     CDS             1885..2760
                     /locus_tag="IV281_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8781573.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            2864..3628
                     /locus_tag="IV281_002636"
     CDS             2864..3628
                     /locus_tag="IV281_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP8781574.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            3645..5057
                     /locus_tag="IV281_002637"
     CDS             3645..5057
                     /locus_tag="IV281_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8781575.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            5074..>5699
                     /locus_tag="IV281_002638"
     CDS             5074..>5699
                     /locus_tag="IV281_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP8781576.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIY"
BASE COUNT         1720 a          965 c         1287 g         1727 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgctgatgtt attccggcat agctcagttg gtagtagcgc atgactgtta
      241 atcatgatgt cgtaggttcg agtcctactg ccggagttct cattttgaga aagagtattg
      301 agcttctttc actctggaga gttgtccgag aggccgaagg agcatgattg gaaatcatgt
      361 agatggtgcc aaactgtctc aagggttcga atcccttact ctccgtagca aatttacctg
      421 gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc
      481 cgtacgggtc attttaattg aatgttggag gtttagctca gctgggagag catctgcctt
      541 acaagcagag ggtcagcggt tcgatcccgt taacctccat aatgaactgg ttccgtggtg
      601 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
      661 gtcgttcttg ttattcaatt aataatttta aaatatggcg cggtagctca gttggtagag
      721 caacggattg aagctccgtg tgtcggcagt tcgattctgt cccgcgccac catggaggag
      781 tagcgaagtg gctaaacgcg acggactgta aatccgttcc ttagggttca gtggttcgaa
      841 tccactctcc tccattttat aggggcatag tttaaaggta gaacagcggt ctccaaaacc
      901 gttagtgtgg gttcaattcc tgctgcccct gccatggcga atgtggcgaa gtggttaacg
      961 caccggattg tggctccggc attcgtgggt tcgattccca tcattcgccc tttttattct
     1021 tggggtatag ccaagcggta aggcaaggga ctttgactcc ctcatgcgtt ggttcgaatc
     1081 cagctacccc agtttctata atttattctt tattttatag attgataata gtataatggc
     1141 ggtatagcca agtggtaagg cagaggtctg caaaaccttc atcaccggtt caaatccggt
     1201 taccgcctta gtactaataa atcaacttgc cggcgtggcg gaattggcag acgcgctgga
     1261 ctcaaaatcc agtgcccgtt gagggcgtgc cggttcgacc ccggccgccg gtattgacta
     1321 agatgtcttc atcttagttt tttattgaga aattaaataa catcttttat gatggcgaat
     1381 gtggcgaagt ggttaacgca ccggattgtg gctccggcat tcgtgggttc gattcccatc
     1441 attcgccctt tattcttggg gtatagccaa gcggtaaggc aagggacttt gactccctca
     1501 tgcgttggtt cgaatccagc taccccagtt attattttta aatttgccgg cgtggcggaa
     1561 ttggcagacg cgctggactc aaaatccagt gtccgttgag gacgtgccgg ttcgaccccg
     1621 gccgccggta taattatcaa tatagcaact ttcttataga gagttgctat attttttttg
     1681 tttttatgta attacctcca aatcaattcg atacaagtat tatcataaaa cgagtaaaaa
     1741 agatattgat aacgctttta ttatgtgtta aactattttt gtaaattatg tagggtgcta
     1801 ctattcaatt aggcacgacc taagaaaggg ttctttctta ggtcgttttt tgtctttaaa
     1861 aattgtttgg agacaaaaat atgtatgaag gagtctgcaa gttgttttgt tttatcatat
     1921 agaggaggtg aattaacggt gtatgtgatg aaaaagctga atcataatgc tgtattgatc
     1981 gatgacgatg gaatagaaaa aattgcgatg gggaaaggga ttggcttttc tgctaaagtg
     2041 agtaagccat ttgatgttga ggatgctgac aaattatttg tattagattc taaagaaaag
     2101 atcaaacgat tcagtgagat agtctcgcag attccaatcg aatttatgga ttttgcggaa
     2161 gaagtgattg aactgataac agctaggatc tcctctccat tagattcaaa tatctatatt
     2221 acattgacag atcatattta ttttgctatt caacgaaaaa gagaagatag agatgtcaca
     2281 gcaattatgt tgccagaaat gagattcctt tatccaaatg aatatgatat agcagtagaa
     2341 gtggtcgagc gaatcaatca tcgatatcaa accgagttag ggtcaaatga agtaggattc
     2401 atcactatgc atatcattaa tgctgagcta ggagagccta atagtttaaa gagcttaaaa
     2461 atagtagaaa tgacgaccac gattttgtcc catctagaaa aatcctattc aaacgtttat
     2521 gataaagaat cactgactta tcatcgctta atggttcata tcaaattcct ggttaaacgc
     2581 ttgatttatc aggaagaaac gccagtagaa aatattggtt tttttactga aacatttaaa
     2641 catagcaaag cgtattgtac agcactaaag attaaagata tcatcgaaca gcaatttcaa
     2701 cttgccattc aagaaaatga aatcatctat ttggcaattc atctagcaag aatctattaa
     2761 aataagggcg taactatcta taggcgcaac caaatgacac atagtaaatg gactatgatt
     2821 atttggttgc gcctatttta gtagaaaaaa gaaggagaat gaaatgggaa aagtgatttt
     2881 taattcggat gattttggct atagccatgg tgtgaattat gggattatgg atgcttatca
     2941 gcgaggaatt ttgacatcaa ctacattgat ggcaaatatg ccgggtttcg agcatgctgt
     3001 gaaactaaga aaggaaatgc ctaggctagg tgtaggagtc catttaacat tgacgtgtgg
     3061 caaaccactc ctcaaaactg tcgatactct tatcgaaagc ggagagttca gatccttatc
     3121 ttattatcag catccattta acattgacaa agatcaactg tatcaggaat ggaatgctca
     3181 gattcaaaaa atttatcatg cggggatcat accaactcat ttagacagtc atcatcatac
     3241 tcatactttg gatgaaaatc aggaggtagt tgttacatta gcaaaaaaat atgatttgcc
     3301 ggttcggagc aactttgaac gaaaagatga agttcgtcat gtcgattatt ttgagccttg
     3361 tttcgatgtc gtaggagaaa ctgatgaaaa aaaacgacaa atcaatacga gtgtggaaga
     3421 atatttggca agtttactca tgatattgag agaagataaa acgatcgaag tcatgtgtca
     3481 cgcagcgtat ttggatcagg aattgttaaa tggttcaagt tttgtgtatc ctcgtaccca
     3541 ccaagtcgaa tttttaattc attcagcatt tgctcaaaaa gtgaaaaatg atcctttgat
     3601 tgagttagta acatatgagg atatttaata gaggaggaat aaaaatgaat gttaaatcaa
     3661 atgtgcagaa gcttggaaga gcgatgctac ttccagttgc agcgatgcca ttagcgggat
     3721 tgatcatgcg tttatcagct gacgatatgc tgaacatccc agtaattggt gcagctggaa
     3781 atgccgtttt tggaaacttg gatatcttat ttgctatagg agttgcaatc ggttttgtca
     3841 aaaataagga caaaggaata cctgcactta ctggtttttt agcgattgct accttgaaaa
     3901 aaggcttaga aattatggac ccagaagtga atatggggat ttttggtgga attatttccg
     3961 gtttagtagc ggcatggacg tataatcgct ttaaagaaca aaaattacca gtagtttttt
     4021 cttattttgc gggcgaaaaa ttcccattga ctatggtgat gattctacaa acaatcgcag
     4081 ctgttttttt gggctttatc tggccgctca tccaaattgg gattgataat tttgcaaaac
     4141 tgctcgttga tatgggagca ttaggtgtgg ggatatttat gtttttgaat cgtttgctga
     4201 tcccctttgg actgcaccat gtgttgaata cgtatattta ttacgattta ggaagctata
     4261 catctcctag caaggaagtt ttcagaggag agatgacacg ctttatcaac ggagatcctc
     4321 aggctggact gtttttatca ggtttttttg tagtaatgat gtttggtata ccagctatat
     4381 gtctagctat ctataaagca gcttataaag aaaataaaga gatggtcaaa ggaatcatgg
     4441 gaagcggtgc aggaacttca tttattgcaa atatcactga gcctgttgaa tttagtttca
     4501 tgtttctttc tcctatgttg tacgtgatcc atgcactttt tgccggttta gcagggattg
     4561 tctgttactt attcaatata cgaattggtt ttacattcgg agcatgtatt gttgattatc
     4621 tcatcaattt tcgaatcgct accaatgcta ttttgattct tccaatcgga attttctttt
     4681 ttgccttgta ttacgttact ttttattatt tgatcaataa gagaaacatt cagacattag
     4741 gacgtgaagc taaggcagag tttggcaatg aagtaacctt agaagaaaca gagttagggt
     4801 tagcaagtaa aaattatgaa tatatggcaa caaaaatgct acaagcgttt ggaggaaaag
     4861 ccaatatcct ggatgtctat agttgtaata cacgcctacg ggtagaggta gtggatccca
     4921 ccatggtcga ggaacagcga atcaagcaat tagggataag cgggatcatt aaaccaactg
     4981 aaaaaaatta tcaaattatc atcggcttag aagtgactta tgttatggca gaattcaata
     5041 aattattaga agaatagaaa ggaaaagatg atgatgaaaa aacagattat cactatagct
     5101 ggaggcggga gtacgtatac accaggaatc gttcaagcca ttttgaacaa tgaaaaacgg
     5161 ttgccattat ctgaaatacg tttatatgat attgatgaag aaagaaatat ggatatgtac
     5221 ttgattgtta aatttatgct gaaaagaaaa ggtttttcaa atattcgcat tcgtgcaacg
     5281 gatgatccaa agcttgcttt tacaggatgc gattttgttt tctcacaaat tcgtgttgga
     5341 ggattggaga tgcgagaaaa ggacgaaaag attccactta aacatggatt agtaggacaa
     5401 gaaacgtgtg gactcggagg atttgcttat gggatgcgct caatcaaagg tttattggaa
     5461 attgtcgatc atatacaaga ctatgccccc aaagcatgga ttttgaatta tacaaatcca
     5521 gagtcgatcg tttctgaagc tgttcgtcgt aaatatccga atgtaaaaat gattaatgcc
     5581 tgtgatatga ctatttcaat tgaagaaacg attgcagtga attacggtta tgatcgtaag
     5641 aattggatcg ttacgtatta tggtttaaac cactttggct ggtatacatc gatttatga
//
LOCUS       DACDIH010000117         5418 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDIH010000117 DACDIH010000000
VERSION     DACDIH010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.118"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(267..1166)
                     /locus_tag="IV281_002639"
     CDS             complement(267..1166)
                     /locus_tag="IV281_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP8781577.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1176..1532)
                     /locus_tag="IV281_002640"
     CDS             complement(1176..1532)
                     /locus_tag="IV281_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8781578.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1887..2183
                     /locus_tag="IV281_002641"
     CDS             1887..2183
                     /locus_tag="IV281_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP8781579.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2827..3780)
                     /locus_tag="IV281_002642"
     CDS             complement(2827..3780)
                     /locus_tag="IV281_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8781580.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3803..4558)
                     /locus_tag="IV281_002643"
     CDS             complement(3803..4558)
                     /locus_tag="IV281_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8781581.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4555..>5418)
                     /locus_tag="IV281_002644"
     CDS             complement(4555..>5418)
                     /locus_tag="IV281_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8781582.1"
                     /translation="HFACALRSKKIFAFILVSLAVSSATISFQTITHNQFLTPSVLGL
                     ESLYVLVQTVLFFFIGGVTMLQQTGTVFFLLNVGLMVGLSLLLFFFLLKKMRANLFLL
                     LMSGMILGTLFGSVSTFLQVIMDPNEYDLLQGRLFASFGNIQSQYLLPASLIIIGCWY
                     LLFRYRHVLDVFHLGNEQAVNLGIDTDRFEFLILCCVSLLTGTSTALVGPVTFLGFIV
                     ANISYRVFSTYRHGQLFIGSFLVAMLFLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFV
                     TKLIRERKSIL"
BASE COUNT         1671 a         1075 c          921 g         1751 t
ORIGIN      
        1 actctcgact tgactgccta gacttcaatc aatattaatt ataattaaat aaaaaatgta
       61 tattatattt tttttgtgat tatcgttcaa aaaaagaata cacgcttgca tctagtactc
      121 tgcgagttac aaatcatccg atcaaataaa cattcgctga ttattttccg caaaaaaagc
      181 agcatatcca cgataaatgg atctactgct cttacttatc cttatgtttt acaaggtatt
      241 ttcttttaca cattactaat gctgtctcaa tacggaacga ttgtcatcgc ttgttcaaat
      301 tcctcttgca aatgttgtgt ttgataggcc tgaaaagctg ggtgatgggc acgcaagagg
      361 acacgtaatt tcataggatc atagctgtct agcaactgtt ctttcaagac aaaatctcgg
      421 gtcagctgcc attcataata ataaccaaaa ggtctgcagc tgtaaatcgt gtgaccgatg
      481 gatttatctt caaagcgacg atgcgtatgg ccaaaaacta cttgtttgat atttgaataa
      541 cggtcaagta attctccgaa tgattctgat cctaaaaatg catttaattt gttccaacgc
      601 tcataaggag cagattgata catgataaat gcccgtttag gaacgaaatg agtggataaa
      661 atcacctcaa gtttttttgc ttccagctgg tctagcaaat tttttgcagc tgcaagcatt
      721 tctgcattga cctcgagatc tgttttccca cgctggatga accgatcata ccaatacaaa
      781 cgcttcattc gtacgattgc ctcgtaatct ttcaattcgg aaaactgata gtcgtaccag
      841 ccattcatcc ctaaaagaac ggtctgctca tttaaagcaa tataccgttc atttaaaaaa
      901 tgtgggtctg gaaaatggtc catcatctgt tcattgggca catctgccaa ctcgtgattg
      961 ccgaaatgaa atgtcgtaga taatccttta ttgacgaaaa attcgacaac agctaggcaa
     1021 cggtctactt tattagccgt atctccggct agatgaagcc gagtgatctt gtgcttttgc
     1081 aaaacttgcc aaagctgcat cagctctgat tcgccgaatt tattgatgtc cacatgtagg
     1141 tcgctgatga tcgccaattt tcccatttta tccctctagt cttttcgaac aacttgcaag
     1201 ttgccttttc tcaagccgct tggtcctttt aattgtaaac tattataagt tggttctaaa
     1261 gcaagtgtgg atggatcatc catgtaaagt attgcactgt ctttgatttt gattggtcca
     1321 actggtgtat cagccactac agaataaaga cttagatctg tttgtgtatc ttttttgaca
     1381 atgatcaatc gaaatgccgt atccaattgg caactgactt ggctttctgc aaatggaccg
     1441 tcaccgtttt cgatatctaa taaaagttga tcgtctgctt ctaatttatt cttcaatact
     1501 tcttgtgctt cttttgtcac ttctaatttc atctgtatca cctcatgtca tagaataatc
     1561 actttattca tcagatgcaa ctaatttgtc tggttctgac tgttctcaat ttattggaaa
     1621 gtaagtcatt cgtttattat gatttctctt atcacaaaag agacaacttt cccttttctt
     1681 cgttcagttt cttgaacgga aaaagaccca aaaagcaacc tgttatttgt ttaaccttat
     1741 tcctttctta aataacaaaa aatactataa aaaataaaaa acctttaaca actattggaa
     1801 aaagaattac tttttgttca cggattatta acaactcttc ggtagagtaa acttatcaaa
     1861 taaaagactt ctttggagga atcactatga aggaatatat aaaaaacatc tattttatcg
     1921 aagaaacaca aaatatcgag ggttcttata ttgaagtaaa aacactcttt gtcaatgaag
     1981 ataaaaccaa agcattggat atttataaaa aattagcttc aaaaaaaacg aatagttttg
     2041 gattgatatt aagcgagtat aaaatcaaag cagaagagag ttatttctat caattactaa
     2101 aacggtggag taagcttccc gccgattttt atcgaaaaat gcagatcatc aactatcaac
     2161 cgcttgctga gacacatgcc taaatagagg ttgtgaaatc agtgctttga gctaataccg
     2221 aaggtcagct gattgaacat tgtttagaag agattgtgac aactgcgatt tgctttgagt
     2281 aataagaacg ctggacagaa aatagcttct catattttct agtctagtgg gcttattgcc
     2341 gagaagcagc agatggaaca tcgtttagaa gagattgtga caactgcgat ttgttttgag
     2401 taataagaac gctggacaga aaatagcttc tcatattttc tagtctagtg gacttattgc
     2461 cgagaagcag cagatggaac atcgtttaaa gaagagatcg gtacaaaata aaaatatcta
     2521 aatttaagtt ttatctaagt ttcgctttgt acctttgatt caaatcaaag gaggcgatct
     2581 tgatgctgaa aaaatggatc acactatttt ttgccactcc ccattctaga atcaacccgt
     2641 atgatgtaga taatgaattc acgttgaaaa aagatttctc aaataaataa aaaccatccg
     2701 taaaatcatc aatacgattt tacggatggt ttttgataac ttctttgagg ataggctctt
     2761 gctatttaag aatgtaagaa acattattgt tacatagtat gagagtttaa tcctctgtta
     2821 tttatgctat ttaaatgctt gattcacatc ttcgatcatc agtttcattg attctaaacc
     2881 gcccccgctt aaataccaga catctggttc aagggagatg atctgctgat ttttaccagc
     2941 attcgtttgt gctaccagtt cattggcaga aatatcatct ttgctgtcgt ctccaccaat
     3001 agcttttgtt ctgtctacta caaataagat atccggattt ttttccagca catattcata
     3061 agaaacactt tgaccgtgag tggatgcttc gatttgatca tctgcttgtt caaagccaaa
     3121 taaatcatgg atgaaaccga atctagaacc agatccgtag gctgataatt gcccttcatt
     3181 cacaagtgtg actagtgctt ttttgtcagt agcttctgct ttctttttcg tctgatcgat
     3241 tgcactagat aattcctcta attgagtttt agcttcttct tgcttaccaa agatctcccc
     3301 taaagcagtg acattttgct ggaaagattc ccatggattt ttcgcatcta aagaaaggaa
     3361 aatggtcgga gcaatttgac ttagatcttt ttgaaaatct ctttgtcggc cagaaatgat
     3421 gattagatct ggctgaagct gattaatttt ttctaagtct ggttctttta ttccacctgc
     3481 tgattctact tttttatagg atgaaagata ctcaggaagg ctgcttgtag ctgctccaac
     3541 taccttatct ccaacaccaa gagcatccat cgtatctaaa gaaccattgt cgaatacgac
     3601 gactttttct ggattatttg gcacctcgat ttggtctcca ttactatctg ttatcgtcaa
     3661 tgtagtttca gtttgtgtgc tgcttttttt tgtgtctgcc tgactggtgt tgttgctatt
     3721 tccacatgct gctaaaccaa atgatgctag aatcaagata cctactacta attttttcat
     3781 ctcttctctc ctaatgttag ttttagaaat aaagacagaa tctgcggcct tcaatctcac
     3841 aaatccgtat gtccatttcg tataattcat ccaatacatc tttttttatc atttcttggg
     3901 tcgttccttg cgtaaataac tgtccatctt tcattgcaac aatttcgtct gcataacttg
     3961 cagcaaagtt gatatcatga agaacaataa caattgtttt cccgatttct tgtactaaat
     4021 tttttaaaat ctgcatgatt tgaaccgcaa aattcatatc tagattgttc aaaggttcat
     4081 ccagtaaaat ataatccgtg tcttgcgcta gcaccattgc aatatacgca cgttggagtt
     4141 gaccgcctga aagagtgtca atcgattgat ccgctatttc ttctaaccca atgtgttcca
     4201 gtgattcctg aacgatttcc tcgtcttttg tcgtcaattt tcctttcgta taaggaaatc
     4261 gtccaaattc tactaattct ttcacggtga tttggacatt tatttcattc gattgtcgta
     4321 ggatcgataa ttttttggct aattcattgg atttccaagc ctttacttcg ttatggtcta
     4381 aatagatttc acctgtatct tttggtatca aacgactgat catcgacaac accgttgact
     4441 ttccagcacc atttggaccg ataaaagcag tcaacttctt ttcaggaatc gttaactgta
     4501 catctttaac cactgatttt ccatggtaca gtttattgac cttttgtaaa tcaatcatag
     4561 gatactcttc ctttcgcgaa tcaattttgt gacaaaataa attcctccga caaattcaac
     4621 gacgacactc actgttgtat tccagccgaa aatctgctct actgcgagct gtcctcctgt
     4681 cagaaacagc atagctacca gaaagcttcc gataaatagc tgaccatgac gataagtaga
     4741 aaagacacga taactgatat ttgcgacaat aaaccctaaa aaagtaactg gacccacaag
     4801 agccgttgat gtccctgtca aaagactgac acaacataaa atcaaaaatt caaaacgatc
     4861 tgtgtctatc ccgaggttta ctgcttgttc atttccgaga tggaaaacgt ctagcacatg
     4921 tcgatatcga aataacaggt accagcagcc gataatgatc aaagaagcag gcaaaagata
     4981 ctgactctgg atattgccaa agcttgcaaa taatctgcct tgaagcaaat catattcatt
     5041 cggatccatg atgacttgta aaaaggtact gacactacca aaaagcgtac ctaaaatcat
     5101 ccctgacatc agtaataaaa aaagattcgc tctcattttt ttcaaaagaa agaaaaataa
     5161 caatagactc agcccgacca tcagcccaac gttcaataag aaaaaaactg tacctgtctg
     5221 ctgaagcatc gtcactcctc cgataaagaa gaaaaggacc gtttggacaa ggacatataa
     5281 cgactcaagt cccaatactg aaggagtcaa aaactggtta tgcgtaattg tctgaaagct
     5341 gattgtggca ctgctgacag ctagcgagac aaggataaat gcaaaaattt tcttactcct
     5401 caatgcacat gcaaaatg
//
LOCUS       DACDIH010000118         5019 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDIH010000118 DACDIH010000000
VERSION     DACDIH010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.119"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..69)
                     /locus_tag="IV281_002645"
     CDS             complement(<1..69)
                     /locus_tag="IV281_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP8781583.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVG"
     gene            293..1093
                     /locus_tag="IV281_002646"
     CDS             293..1093
                     /locus_tag="IV281_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8781584.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            1112..2980
                     /locus_tag="IV281_002647"
     CDS             1112..2980
                     /locus_tag="IV281_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8781585.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            2973..3464
                     /locus_tag="IV281_002648"
     CDS             2973..3464
                     /locus_tag="IV281_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP8781586.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            3452..4006
                     /gene="srlA"
                     /locus_tag="IV281_002649"
     CDS             3452..4006
                     /gene="srlA"
                     /locus_tag="IV281_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP8781587.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4024..5019
                     /locus_tag="IV281_002650"
     CDS             4024..5019
                     /locus_tag="IV281_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP8781588.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         1753 a          793 c         1042 g         1431 t
ORIGIN      
        1 gcctaccaac agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa
       61 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
      121 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
      181 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
      241 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
      301 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
      361 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
      421 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
      481 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
      541 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
      601 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
      661 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
      721 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
      781 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
      841 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
      901 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
      961 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     1021 ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa
     1081 aacaagagga taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt
     1141 attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa
     1201 aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat
     1261 tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt
     1321 tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag
     1381 aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt
     1441 gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga
     1501 gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac taagaataat
     1561 aggagatgag ctagaattac gactactttt attttatttt gtttctccct acttttcaga
     1621 gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac
     1681 gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag
     1741 aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata
     1801 tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag
     1861 ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat
     1921 gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg
     1981 acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct
     2041 ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga
     2101 gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga
     2161 agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga
     2221 gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac
     2281 gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga
     2341 tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt
     2401 cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt
     2461 gactaatctt tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg
     2521 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     2581 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga
     2641 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     2701 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     2761 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     2821 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga
     2881 actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     2941 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     3001 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     3061 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga
     3121 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     3181 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     3241 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     3301 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     3361 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     3421 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     3481 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     3541 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     3601 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     3661 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     3721 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     3781 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     3841 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     3901 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     3961 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     4021 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     4081 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     4141 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     4201 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     4261 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg tttccacagg aaaaagtggc
     4321 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     4381 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     4441 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     4501 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     4561 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     4621 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     4681 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     4741 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     4801 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     4861 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     4921 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     4981 atcattgctt gggtagcaag ttttggatta tatagctga
//
LOCUS       DACDIH010000119         5016 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDIH010000119 DACDIH010000000
VERSION     DACDIH010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.120"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(80..1888)
                     /gene="pepF"
                     /locus_tag="IV281_002651"
     CDS             complement(80..1888)
                     /gene="pepF"
                     /locus_tag="IV281_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP8781589.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2064..3062)
                     /locus_tag="IV281_002652"
     CDS             complement(2064..3062)
                     /locus_tag="IV281_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP8781590.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3288..3941)
                     /locus_tag="IV281_002653"
     CDS             complement(3288..3941)
                     /locus_tag="IV281_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP8781591.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="IV281_002654"
     CDS             complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="IV281_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP8781592.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1486 a         1034 c          765 g         1731 t
ORIGIN      
        1 aaaaaatacg tcgactccct tacaagagag ccgacgtatt tttttcgttt ctattcgaga
       61 aaatcaaaaa tagaaagcct tataaacgat cgatcaattc ttctaattca tttagacgtt
      121 gttcaaacat cgacattgca tcttcaatat atgcagcctg tgtcatatca actcctgctt
      181 ttttcattac ttccacagga taatcgctgt tacctgcttt taggtaagcc aagtagttct
      241 ctagtgcctc tggttcctgg tttagtattt tcttcgccag tgctgaagca gcagaaaagc
      301 cagtcgagta ttggaaaaca tagtaattat aataaaaatg cggtatccgt gaccattcga
      361 atttaatttc tgggtcttct tcgactgctg gaccataata ttttgcattc aatttaccat
      421 aactatcact taggtattca ctagtcaatg gcacaccttt ttcatcttcc gtatgcatga
      481 aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt tcctttaaac ccatccaagt
      541 aatggttgag tacataagcc cgtacacgag ggtctttttc tgtttccaat aaatactccg
      601 ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa aaagatggag tagtcgccgt
      661 acacataagg ctggttcgaa cgagtgaaat aactatgaac actatgtccc atttcgtgga
      721 caagcgtaaa tagctgatcc agcgtatcat gccaattcaa taaaatatat ggatttgtgt
      781 catagcttcc cgaagaataa gcaccgcttc gtttcccttt attttcgaca acatcgatcc
      841 aacgttcaga gaatgctttt tctacgatgg ccatgtattc ttcacccatt ggtttcagtg
      901 cttctaaagc tttttctttt gcttcttcgt acgtaaaggt aattggagct tcccctaaga
      961 ccggtgtata aagatcatac atgtgcagtt tttccacttc taataaacgt ttccgtaatt
     1021 ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac gtctaccaaa gtatcgtata
     1081 cactttcagg aatatgatta ttgctcaaag acgcttctct ggcagagctg taattacgga
     1141 ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa agtagaagca aatgtatttc
     1201 taaattgttc gtaaacactg tacaacccct taaatgctgc ttcgcgcacc cttcgatccg
     1261 tgctttctag caactgacca tacacgccat gagataattg gactatttca ccattttctc
     1321 cttcaatcgt tggaaaaact agatctgcat tattcaaaac agcaaatgta tcacttgaag
     1381 catcaaagat ttcacctgct ccagcaagaa gagattcctg ctcagcagat aagacatggg
     1441 ctcgattatc tactatttgc tggatataat ggcgatagac ttccaatttt ggttcttctt
     1501 tgaaatattg ccaaatctgg tcatctgaca attgcaatat ttctggttca aaccaggaaa
     1561 cggcttcact aactttcgaa aacagactgc ttgctcttgc ataaagcgct tggtaatctg
     1621 tatttccagt gtcttgatcg tttttaaggt gcgcatatac ataaatgact tcagtttggc
     1681 gataaaccct caatacgaat tcaatcgcat ttaaaaattg agaagcgcct tgatcaagtg
     1741 ttcctttgtg tttttcagat tgttttaact cttctgataa ttccaaatat ttctcatcaa
     1801 actcttggtc gcttgaaaag atcttggtca agtcccaagt taaattttca ggcaattctt
     1861 ctcgttttgg caactgcttt acttccatga aatccactcc ttttcttccc ttagctctat
     1921 ctatcaaaat agatcatagt ctaacatcca catgtcacag catctaactt ttctactcat
     1981 tcatgtgtct gcttccatca attttagcat aactaaatct tgttttacat agaaaaccga
     2041 ttcctagcct atttttatac ttactatttt ttcaaagaag acaagtgtat gcattcgtta
     2101 aaaaactgct gatagaccat ctcttcatca atcataggaa aaagccattc ccttttatgt
     2161 tcttttattt ggcaaagcca tgagttgaaa tcaggaactt tctggttttc caaggcttct
     2221 tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat aaaagaaaaa ccgacttttt
     2281 tgatagatcc acgatgatag atgcaaaaga tgtttatgac gcaagtagca ttgttcttgt
     2341 atggatactg tattcttttg cttgtgaata agttggcgtg tcagccattt cttcccatct
     2401 tcactcactt tttctatttt tttcatttgg ttcattttaa tttcgttact agtgaatatt
     2461 tctttaaggt ccctactaga aaaagtccag cagctactgc aaaaggaaat atgccctgat
     2521 acagtctctg taatatgatg atacaaataa agcttttgtt tcttgagatc agctttccag
     2581 caatgcactc ctctttttct attatagtag cagcaacttt tttctatcaa tgagaattgc
     2641 gattgtccaa gaaaagaatt accaagtatc caccatactt ggtaactgtt attcaaataa
     2701 ttttgggttc tttcaataaa tctctggtga gacagtctag aacattggat ttctattgct
     2761 agattcgcca acaaaatatc cggcctttgc cgcaacctcg gcaaataggc ttccaaaaat
     2821 actggttcta ctgactgtcc cagccaatcc atgaagcatt cctttaactg taagtgctct
     2881 tttgattctc tttcactaaa gaccgaacag ttatctgatt tttgatgggc aaaatgagca
     2941 cattttttct ttcccttttt caaaatcact ctttttcgac agccacgaca ataatatttc
     3001 tttccttttt ctgcatgttt agcaaaacag cacttattat attcattcat tgcaagcagc
     3061 atttttctcc ctctgaactt atttacgtct aagaatttat ttacacgaaa aaagagggcg
     3121 tgaaaaagct attctgcatc aagtaataag aacagctaaa caaaaatagt tcccaatatt
     3181 tttcgtttgg ttgggcttgt taccgcagat gcaagctttt gaacacattt atacgaaaaa
     3241 aagactgcga cacaattttt gtcacagtct cttactataa tgctgattta tacaaaataa
     3301 aatttcgttc gctcgatagc attacgcgcc atcagacatt gtccatattc attcaatgtt
     3361 tctggtgtac gatcgctctt ttcagcaaac tccaaaattc gagcaatctg attctctaca
     3421 tccgcttggt tgagattttc catccagaag tgaacgacta ggtaatagcg ctcttcgaat
     3481 aaatacaaat cagtccaagc agactctagt tcgacatctg ttgcaagttg gatcacttgt
     3541 tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt cttctagggc gcttccagat
     3601 aattcttcct ccggatcgct gccaataatc tgctgacgaa gaaagtcact cacatcttct
     3661 gaatcaacag tagaatcttc tgattgagaa atttcttcat ccggaagatt tttactgata
     3721 aacagttcta atccgtctcc ttttggcaaa acttggaaag tgacagcttc gcttcctcgg
     3781 aactcctctt cgatatcaac ttcttccaaa atactataaa agaaattttc gatttctttg
     3841 tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct ctaaatcttc atgaccaatt
     3901 agtacacgga tcgtattttc attaatatgt tccatttcca ttttgctaca cctcactttg
     3961 ccacaatgtt taatatagat tcattgtagc gaaatcagct taaatgtaaa gaaaaaaaat
     4021 ctcgctgtta agcgaattta ataaaaccag cggatttttc aacctttcaa gctatcttat
     4081 ttaactataa catttttggc agacgaatac tatatagccg acttttcagc taattttccc
     4141 tctctatttt tcgtgcaatc atcatcttct actacctttt tatacatcta atcaagaata
     4201 ctttttgtaa aaacaaaaaa acttggaaaa actatctttg taatagtctt ttccaagttt
     4261 cgtattatcc ttctataatc cagccatcaa ttgtgcttga cgtaattcta attgtcgtac
     4321 atcgcgcggt aggaaccgac gaatttcatc ttcattaaaa ccaacttgca gacgtttatc
     4381 atcaatcatg attggacggc gaagcaatcc aggattgttt tgcacaagtt ccaataattc
     4441 ttgtaacggc agatcatcta aatccatgtt cagtttttgg aatactttgg aacgtgtcga
     4501 gatgatttct tctgttccgt cttctgtcat ttgtaaaatc gcttttaatt cagaactgtt
     4561 caatggttct gaaaaaatat tacgttcaac aaatggaatc tgatgttctt gcaaccacgc
     4621 acgagccttt ctgcaggaag tacaacttgg ggaagtatat aatgttaaca acgtgcacca
     4681 ctccttttcg tgataagaat cacttttttg taacagaaga tcataataat ctcccatctg
     4741 tttctattat acataatcaa cttttaaata gctacctttt tttcataatt tttttaaaaa
     4801 aatgaaagtt atctaaggtt aatatacagt aaaaccagca ttctcgcttt ttttatcatt
     4861 taaaaattag agttttcaaa tctaattcaa attttctaaa catatgaaac agcgtataaa
     4921 actgtactat tattttatct caaaagctaa atgaaaccga tttcttaaga ttttttatgt
     4981 tttttatttc tttaatagta atgttttctg tcattt
//
LOCUS       DACDIH010000120         4899 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.121, whole genome shotgun sequence.
ACCESSION   DACDIH010000120 DACDIH010000000
VERSION     DACDIH010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.121"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            218..679
                     /locus_tag="IV281_002655"
     CDS             218..679
                     /locus_tag="IV281_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781593.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            692..1552
                     /locus_tag="IV281_002656"
     CDS             692..1552
                     /locus_tag="IV281_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781594.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            1590..1979
                     /locus_tag="IV281_002657"
     CDS             1590..1979
                     /locus_tag="IV281_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8781595.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1966..4413
                     /locus_tag="IV281_002658"
     CDS             1966..4413
                     /locus_tag="IV281_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8781596.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            4370..>4899
                     /locus_tag="IV281_002659"
     CDS             4370..>4899
                     /locus_tag="IV281_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8781597.1"
                     /translation="MLLIPVRQFKREWSEEMKKKLFRIFGIVLISLVTLIILLSLVGT
                     VAEATGLVDDTVGAGNLYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAI
                     TNFIWTVSLYLSNATGYVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFG
                     FLLLMILALGVYVAYTG"
BASE COUNT         1571 a          762 c         1035 g         1531 t
ORIGIN      
        1 attgcctcga cggaatgtgc aattaatgca ataaaacttt tccataaagt caatcaaaat
       61 aatatgacca atagtactct tttttcatgg ataggagatg ggagtacttt tgagaattta
      121 ggatatcaat atagcgacag aaatactttt cttaatggag tcaataagtt tgaagaaaga
      181 ttcttttcta aatattgcaa agtatgtggg gagttagatg attaaatttt cgtgtgaccg
      241 tattgtcatt gaagtaaggg atgaagttct taagatattt gaacaatatc ggcagggaaa
      301 ggatgacttg cctgaagctg gaggagttct tcatggaaaa agacttaaga atgggagcat
      361 aataattacc gatataacaa cacctcaaat aggagatata caaaaaagat tactctttaa
      421 aaaaaaccga gaaattcatc aacagctttc taatgagaaa tggatagaat caaataagtt
      481 ttctattgca ttgggagaat ggcatactca tcctgaagct gtaccaactc catcaaatgt
      541 tgataagaaa agctggagac tgaacgtgag caagcaacat gatgatagag tttatgtttt
      601 tattatcgtt ggattttctg atttgagaat gtggttactg tcaaaaaaat cagatattag
      661 gcaggctata aaactataaa ggagtgatta gttgaaatca caaaagaata tttggtggtg
      721 ggggttttta gtaggaaagg taatgatacc agcccttctc ttaatttctc taccgatgat
      781 tacttttttc tttaccaata aaaaaatgag taaagaagcc gtgatttttt tctttggaga
      841 ccaaaaagct acctttattg aaacgttagt aacatctctt cagcttaatt tgaattacat
      901 tttttcaatt attattgtta tatcgttatt gaattttttt aaaaaaagaa attcaggaaa
      961 ggtgtttaac tctaatggaa atgtgtacta caactatttt tattttgtat tttgggtagc
     1021 agcaacttta cttggttatg ataaaattca gattgcagga attcctatac acatgcaata
     1081 taagttggtt ttgagtggaa ttttttctga ggtgttgcca gatatatacg atgatcatta
     1141 cgacagtgat ggaacatgta aagtaagcat tgaaaaagaa aatttcgatg acatcgatgg
     1201 atatgactct gtgaacttac tgattattga tacttacgat ataaaaatga gtgaattatc
     1261 tatggaaaat caaacatatc ctacgataat tgttagaggg aattcaatag atggagttcg
     1321 taaagttaat agatctttaa tcttggaaat aaaaaaaaca atggatgaaa ttcaaaaaag
     1381 tgactttaaa aaagtatttg ttgcttcaac atctaatcca aaaaatagta tcaatattat
     1441 taattcatcg tttagatttt tcggtcgatc acgtaggttt aaactttatg ttcttcaaaa
     1501 ggattatgca agcaatggaa agtatagtaa gaaatataga atttttattt aagaaggaat
     1561 ttttccttct ttttattttg gaggaaacta tgaaaaaaat aaaaagctac accagtattt
     1621 gggcagttga aaaagtcatt tatgcgatta atgactttca gctgcctttt cctgtgacct
     1681 ttaaccagat ggcttggttt gttttatcct tgatgtttgt cattgtattt gcccatgtgc
     1741 ctccgctatc catgatagag ggggcttttt taaaatactt gggcattccg atagcggtca
     1801 cttggttcat gtcgcaaaag acctttgacg gtaagaaacc attgggattt ttacgatcct
     1861 ttatcagcta tcacttacgg tttaaggtca cttttgcagg aaagaaagtt aaagaacaga
     1921 agaaacgatg ggacgaacca atcaccttgg taaggagtgt gaattatgta cccgattaag
     1981 tatattgaaa ataatcttgt ctttaaccaa gaaggcgagt gttttgctta ctatgaatta
     2041 gtgccttaca attactcctt tttgtcacct gaacagaagt atcaagtaca tgataacttt
     2101 cgccaattaa ttgcgcaaaa tcgtgaaggt aagatccatg ccttacaaat tgccacagaa
     2161 agtagtattc gtgccacaca agaacgttcg aaaaaggaaa ttaccggtag actcaaagaa
     2221 gtagcaaagc aacgaattga tttacagaca gatgcattag tatccatgat tggcgatagt
     2281 cagattgatt atcgcttctt tattggcttt aaactgattt cgacagatga agaggtcaat
     2341 ctaaagaatc tcaggaaatc tttcttttct ggacttcaag aatttgtcta tggagtgaat
     2401 catcatttga tgggtgattt tgtttcctta tccaatgaag aaattcgtcg ctataccaag
     2461 cttgaaaaac taatggaaag taaattggct cgaagattta aagtcaaacg agtaacgccg
     2521 agtgacttga cgtatttaat tgaacatatc tatggtgaaa aaggcacacc gtttgaagaa
     2581 tatgaattcc agctaccaaa gaaaaagctg aaatcagaaa cgctggtgaa acgttacgac
     2641 ttattacgtc ccactcgttg tttgattgaa gaaaagcctc ggtacttacg gatggaacat
     2701 gaaaatcatg agtcttatgt agcgtactta acaattaata ccattgtagg ggaaatggag
     2761 tttccttcct cggaactttt ctattaccag caacaacaat ttacgtttcc gattgatact
     2821 tcaatgaatg tggaaattgt gacgaataaa aaggccctag ctaccgttcg taataagaaa
     2881 aaggaattaa aagatttaga caaccatgcc tatcaatctg ataatgaaac gaactccaat
     2941 gtattagatg ctttggattc cgtggatgaa ttggaaacca ccttggatca gtcgaaagaa
     3001 tccatgtata aactaagcta tgtggttcga gtgagtgcta aatcagtgga tgaattaaaa
     3061 cgccgctgtg atgaagtgct cgatttttat gacgatacca atgtaaaact agtccggccc
     3121 tttggcgata tgatgggtct acacaaagaa ttcttaccct caagtaagcg atacatgaat
     3181 gactatattc aatatgttac gtctgacttt ttggcgggac ttggttttgg ggctactcaa
     3241 atgctaggag aattagaagg catttacttt ggctataacg tagatacggg gcgcaatgtt
     3301 tacctgaaac cggcattggc atcacaaggg gtcaaaggct cagtaaccaa tgctttggca
     3361 tctgctttcc ttggttcgtt aggtgggggg aaatctttta gcaacaatct cttagtctat
     3421 tatgccgtcc tttttggtgg gcaagctgtg attgtcgatc caaaaggaga aagaggcggt
     3481 tggaaagaaa cgttgccaga aattgcggat gaaattaata ttcttaactt gaccagtaag
     3541 ccagaaaatc aaggcttact tgatccctat gtgattatga agaaaaagaa agactcagaa
     3601 agtttggcga ttgatatcct gaccttttta acagggattt ccagtcggga tggggagaag
     3661 ttccctgtgt tacgcaaagc cattcgaacg gttacccaaa gtgatcagcg aggattatta
     3721 caagtaatcg ttgaattgcg taaagaggac tcaccagtcg cggaaaatat cgcggatcac
     3781 atcgagtcct ttaccgatta tgattttgcc catttgcttt tctcagatgg ttcgattacc
     3841 cagtcgatta gtttagataa gcagttaaat atcattcaag tagcggattt ggttttacca
     3901 gatgcagaaa ccacttttga agaatatacc accatggaat tgctgagtgt ttccatgcta
     3961 attgttattt ccacttttgc cttggacttt atccatagca accgagaaat tttcaagatt
     4021 gtggacctgg atgaggcttg gagttttctt caagtagctc aaggaaaaac gttatcgaat
     4081 aaattagtgc gtgctggtcg ggccatgaat gccggtgttt acttcgtcac gcaaaacagt
     4141 gatgacttgt tggatgaacg tttgaagaat aatattgggc taaagtttgc atttcgcagt
     4201 accgatattc atgaaatcaa gaaaaccttg gaattctttg gattagataa agaagatgaa
     4261 agtaatcaaa aatgcatgcg agatttggaa aatgggcaat gcttgatgca ggatttgtat
     4321 ggtcgagtag gtgtggtcca agttcatcca gtctttgaag aacttttcca tgcttttgat
     4381 acccgtccgc cagttcaaga gagaatggag tgaggagatg aaaaagaaac tttttcgtat
     4441 ctttggaatt gttttaatca gtttggtcac tctgataata cttttgagct tagttggaac
     4501 ggtagctgag gcaacaggtc tggttgatga tacagttgga gccggaaatc tttattccca
     4561 atattcactt ggtaactatc agttggattt ctttgtggat agttcgtggg attggctgcc
     4621 ttggaattgg ggtgatggcc tagggaagag cgtaatgtat ggactttatg ccattactaa
     4681 ttttatttgg acagttagtc tctacttatc aaatgccacg ggctacgtgg ttcaagaagc
     4741 ctataagctg gatttcattt cagatacggc tgaaagtatt gggaagaata tccaaacctt
     4801 agccggcatt accgaaaatg gactccagac ctcgggcttt tactttgggt ttctattatt
     4861 gatgatttta gctctaggag tttatgtggc gtatacagg
//
LOCUS       DACDIH010000121         4870 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDIH010000121 DACDIH010000000
VERSION     DACDIH010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.122"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..344
                     /locus_tag="IV281_002660"
                     /pseudo
     CDS             1..344
                     /locus_tag="IV281_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            444..644
                     /locus_tag="IV281_002661"
     CDS             444..644
                     /locus_tag="IV281_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781598.1"
                     /translation="MLKRIAWDKDNKTVIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            778..2004
                     /locus_tag="IV281_002662"
     CDS             778..2004
                     /locus_tag="IV281_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781599.1"
                     /translation="MIFYMFININEVLKMTTITMTKNIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYRNKSKYGLLPFRGRDIKIHKADKFYISLPKGFRLKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            2929..4253
                     /locus_tag="IV281_002663"
                     /pseudo
     CDS             2929..4253
                     /locus_tag="IV281_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
     gene            4246..4593
                     /locus_tag="IV281_002664"
     CDS             4246..4593
                     /locus_tag="IV281_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781600.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFAELLKWSQI"
BASE COUNT         1888 a          598 c          922 g         1462 t
ORIGIN      
        1 aggaattaga aaagaaaact gctttattaa acgaaatatt tagtgctgat tgtataatcc
       61 aaagtttaaa agaaagttca ttttcaaaac taacagatca gaaaaaaatt tcttatatgc
      121 taagtttaga taatctagga actaaatatg atgaatcggc aagtagcgat aagcttttga
      181 ttgaattagt atcaagtctt agtagttttg gaaaaatgaa aagatttaat gtgatattag
      241 aaatttttgc ttggataatc ccttcattta tttggttttt ttatattttc atagttgaat
      301 ctgagttaat agccaaggtc tctcttgaac tgttaattcc gatatatgca ttttatttaa
      361 taggttcagc aatttacttt aataacttgt atacacgtat tcaaataaaa aagcagttat
      421 tacatgaagg attaagtggt aatatgttaa aaagaattgc atgggataaa gataataaaa
      481 cagttattga gcttaaagaa tataaataca attatttctc gagatgtata gatagaaagt
      541 tactgtttaa aaaagaagga ttagaattta gttttgatga agatgaattt gaaaaattag
      601 tgatggactg gcgtaagaat ttactggata cggtacaatt ctaaaagcaa ttgaggtcga
      661 aattgcgata cttgaatcat ggtctatatt atcgtgttat gagtgaaatt tctaataaag
      721 ataagatatt tcatttggac agtcatgtga atatgagctt aacaattaaa cgtatttatg
      781 atcttttata tgtttataaa cattaatgag gtgctaaaaa tgacaacaat aactatgaca
      841 aaaaatatag aagtatatgt tgatggatca caagaagaaa gggattcctt ttttgatttt
      901 ttttggaaaa atagtagaga atatgtaaaa gcgttgaatc tggtatataa acagctaatg
      961 tttgaagagt tgataaaaga tgcaattaaa gaaatggatc aaggttatgc tgagagagaa
     1021 gaaagtattc aaaatcggat tgaaaagtta gcatatactg atccaataga tcaaaaaaaa
     1081 cttgataaag caaaagctga attatctaaa ttgagaaatg aaaaaaataa agaagcgaga
     1141 gaaatattaa cgcaagcgat agggttgaag agtcaaacga gaacacgaga tgtagcaaaa
     1201 aaattagact ttgactttgg agattttgtt gattcagcaa atatgaaagc tagtaaggat
     1261 tttggcaatg attttattgg aattgttact ggtcaaagaa cacctagaat ctatagaaat
     1321 aaaagtaaat acggattgtt accgttcaga ggtcgggata ttaagattca caaagctgac
     1381 aagttttata tctctttacc taaaggattt agacttaaag taaatttagg tagcaagcca
     1441 agaaaagcaa gagatgttgc ttcagtttta caacacatta tggatggcga ttacaaatta
     1501 aatcaaagta atcttatttt tgatggaaga aaattatttt ttaatctaac aatttctttt
     1561 gaaaaaagct ataatgaaat ggaattcaag aaaggtaatg aattgatcgt aaactttgca
     1621 gatttgtgta gcggtgaatg tttaattaat gataaatctg taatgtcttt tggtgatagc
     1681 caatatataa gaaacttcaa aagtaaaatt ttagcaatga aatcaaaaga acaaagccga
     1741 ggagtctttt ctaagggcgg tcatggtcga aaaagaaaat tagatcaaga tcgatgggaa
     1801 gcaataaaga aaagagaaag taactttgtt aaaacttata ataatcaatg ttctgcaaaa
     1861 atcgttaaaa ctgctattcg caataaagct caaattattc gtatcattga accagataag
     1921 gcttatggcg aatgggctta ctatcaattg aaagaacaac tagtaaacaa aggaaaaaga
     1981 tatgggatag aagtcagaag ttagtaattt aaacaatcgt tgatttggca acgaaatagt
     2041 gagggaaaat ccactcactg aagggtaaac tccgtcaagt tgtgtgacca gacggaagga
     2101 cgaatgttgt tgaggtttta gaactatata aaacagcatg tttctcaaat tatgagatgt
     2161 taagaaaaat gattcgagag gttttagaac tatataaaac agcatgtttc tcaaacaaag
     2221 tgcagaacga aaatcaattc atcacagttt tagaactata taaaacagca tggttttaaa
     2281 acaagaaata agtcagtttt ccttttgctt tagtaaggaa aaatagagta ttctcaataa
     2341 aacttaaaat aagaaagaat aagcaaacct ttgttttaat agctaatcga agtaaaaccc
     2401 aaagccatat gaactcctga atcagcgaaa gtttatgatg attgggctta ctaataattt
     2461 aggaattaga tggtaaatct aaattaaaca atcgttgatt tggcaacgaa atagtgaggg
     2521 aaaatccact cactgaaggg taaactccgt caagttgtgt gaccagacgg aaggacgaat
     2581 gttgttgagg tttagaacca cataaaacag catgtttctc aaattgcctt agtggttgaa
     2641 ttgagatcta tcacgtttta gaaccatata aaacaacata gttctcaaat tgtttaaaat
     2701 gattgtcaag ttaaaaaaat ctgggatcat ataaaatagt atagttctca aatatgatta
     2761 gaaaccctat taaagagcaa tattaaacca acttacttaa atcgtttaat taagaacagt
     2821 aatctctttt agtcagactt ttagtctgac ttttttgtat ttccaaaatt tattctttac
     2881 aaaagcgaac gtacgtttgt ataattgctt gtgaatgggg gaataaaaat gtttgattac
     2941 agtagagaac cggtgaatga ctatttttta atcgacatga agtctttcta tgctagcgtg
     3001 gaatgtattg aacgaaattt agacccaatg actacaccgt taattgtcat gagtagatcg
     3061 gataacactg gttcaggtct gactctagct tcttcaccta ctgcaaagaa aaaatatcat
     3121 atcactaatg ttagtagagc taatgattta ccttatccat taccaaaaga actttatatt
     3181 gttccacctc gaatgaactt atatataaaa aaaatatgga agttaatcag atttttagaa
     3241 agtatgtcgc agatgaagat ttattgattt attcgattga tgaatcaatt ttaaaagtga
     3301 caaaatcctt aaatttattt actagttcag aaacaagaag cgagaggaga agagaattag
     3361 ctaaaataat caaagatgag attgagaaag aaacaggact aatagcaaca gtcggaatag
     3421 gagacaaccc attattagct aaactcgcac tggacaatga agcgaagagt agtaaagaat
     3481 ttatagctga gtggacttat gagactgtac ctgaaaaaat atggaaaata aacgagatga
     3541 ctgacttttg gggcattgga tcacgcatga aaaaaagatt gaatcatatg ggaattatga
     3601 gtatagaaga tttagcaaat tggaatccct acacaattaa aagtcagtta ggtgtaatcg
     3661 gacttcagtt atatttccat gccaatggaa ttgatcgtac ggatattgct attccaccag
     3721 aaccaaccaa agaaaagtct tatgggaatt cacaagttct accaaaagat tatacgagga
     3781 gagaagaagt agaactagta gtcaaggaga tggcagagca agtagccatt cgaattagac
     3841 aacataattg caaaactggt tgtgtacgct tagcaattgg tacgtcaatt ttggaagaaa
     3901 aatcaggttt ctcccaccag atgaaaatac caattacaga taatacgaaa gaattacaaa
     3961 attattgtct ttttttattc aataagtttt atgaggggca ggaagtaaga catataggag
     4021 taacttatag cagattagta tacacagatt ccctacaatt agatttattt agtgatccaa
     4081 gaaaaaagat tgctgaagaa aatttggata ggattattga taagattaga caaaaatatg
     4141 gatttacagc tattatacat gcttcatcta tgttagaagg tgcaagaagt attacgagaa
     4201 gcactttaat aggaggacac gcaggaggat taggaggttt gcataatgat tgaatataac
     4261 gatagaggac gtttaaaatg gtcgggattt tatttgtctg atcatactga aagaatcaat
     4321 gcagacaatg tacaacgtgc taatcataat ttggcaaaag aacaaatgac gactgaagag
     4381 attgcgaaaa tactgagaac tgctgtcata aaaaatagac ccgtctgcat tcaaaaagag
     4441 gaacgaaacg ctgaaggtta ctatccagat gatgtagtag gaaaaattat tggatatgac
     4501 gagcttggtg tatttgtgga agaagaaaaa gttcactatg atgaaattag gaatgtttct
     4561 ttcgcggaat tattaaaatg gagtcagata tgaacattat taaaaaatac gaattcggtt
     4621 atattacgta tgaacagcta atagaggaat tatggggata tggtcaacaa ttaattaatg
     4681 aagtggggat tgattgcttt tgtttctata ttgaatccgg agcaggatac cataagaaca
     4741 gatactatat atttccatat ccaccaaaat agtctcttag agatttttaa attttctcca
     4801 aatactttca attattgtga aatatttccc tttaacagaa ttaggcagaa gtcgcccatc
     4861 ctctataggt
//
LOCUS       DACDIH010000122         4803 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDIH010000122 DACDIH010000000
VERSION     DACDIH010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.123"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(54..1202)
                     /gene="nagA"
                     /locus_tag="IV281_002665"
     CDS             complement(54..1202)
                     /gene="nagA"
                     /locus_tag="IV281_002665"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP8781601.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1278..2114)
                     /locus_tag="IV281_002666"
     CDS             complement(1278..2114)
                     /locus_tag="IV281_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8781602.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2298..3515)
                     /locus_tag="IV281_002667"
     CDS             complement(2298..3515)
                     /locus_tag="IV281_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8781603.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3898..3981
                     /locus_tag="IV281_002668"
     tRNA            3898..3981
                     /locus_tag="IV281_002668"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3932..3934,aa:Leu,seq:gag)
     gene            complement(4221..4799)
                     /locus_tag="IV281_002669"
     CDS             complement(4221..4799)
                     /locus_tag="IV281_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781604.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1457 a         1001 c          775 g         1570 t
ORIGIN      
        1 aaaccttaaa accaaactta agaagtttag ttttaaggtt aacttaaatt atattatgcg
       61 tggaaacgtt ccacgccatc tagataagtt gcttccaatg tcatatcagg gttcaatacg
      121 ataaagtctg catcgcgtcc ttgagcgatt tttccacatg tatcatcgat tttacagctg
      181 attgctggaa tcagactagc catcatgact gcttgttctg gtgttgctaa tccccagtct
      241 acaacatttt taattgcttc tttcaatttc aatatacttc cagctaagtt gccagattct
      301 agacgtgctg ttccttcttt tactactact ggaaattcgc ctaaattgta atttccatca
      361 ggcatcccac cggccatcat acagtctgta attaatgcga catgatcatg tcctgctttt
      421 tccattagga ttcccgcagc atttggatgt acatgatggc catcacaaat caattcagaa
      481 aagacatgtt ctaaagataa taacgcaccc accatacctg gttcgcgatg attcaatcca
      541 cgcataccat tgaaagcatg gacaaataca cttgcgcctg cttcaacagc tttttgcgct
      601 tcttctagtg tagcattact gtgtcctaga gaaacaacta ctccttcttc agtcacttgt
      661 ttgacaaact cttctacacc ttgacgttcc ggtgctaaag caattttttt gattaaacca
      721 cctgaagctt cttgccattc atggaaagta tccaaatcag gatccccgaa ataacttggg
      781 ttttgtgccc ctttgtgttc ttctgtgaag aaaggacctt caaaatagat tcctttgatt
      841 ttagctcctg gtgcttcctg atacattttg ccgattgtct cagctacatc ttttaaacgt
      901 tctttgcttg acgtcaatgt tgttggtaaa aatgaagtca ctccacaaga taacagacct
      961 tcagacatca ctttgatgcc ttctgcatca ttatccatta catcatgatt catatagccg
     1021 tgaatatgtg tatctaccaa accaggagca atccatttgc cgctttggtc aatgatgttg
     1081 acttctccct caggtagttc tttggtgtat tcaccaaaaa gtccatcggt aatttcaaga
     1141 tagcctgcat gtttgacacc tgacgctaaa aagaatttgt cagcatagat aaaagttctc
     1201 attctattct ctccctttgt ttttctacct ctaaagtact cttatttaga gaagaagtca
     1261 agaatggtct acaccattta caaatttttt gaaatttgtt tcacagcttg tgctaagcct
     1321 tcatagaatg ctgctctttc tggttcttct ttacgcttgt atattaagct tttaagcaca
     1381 aataaatcat tgaggtaata gtagctattc ttctcttgag cccaatagat tccttgattc
     1441 acctgttcta acgcttcgtc gatttcattt tgatggaata agaaatttcc ataattataa
     1501 aaaatttgcg aaaattcact ttttgatcgt tcatcagctg attgatgcag cagttggatg
     1561 cttcgactca aatagtagcg tgcttcagcc gtttttcctg ccacaccata tatttttccg
     1621 atacaactaa tcagttgtat ttctgtgtca gaaacatgga ttctttcttt ttggctagag
     1681 tagcttaatg cttctttcaa ataataaagg gactgagaga tattttgacg caacgtgaat
     1741 tcgcaaacac cgagataata ataatagcgt tgaaagtcag ttgcttgata cagattttca
     1801 gaaatatcag aagattttag tacttgttct aacttccgat aatcacgttt ttggaaacaa
     1861 ctatccaaca gttcaaataa tttatcgttt gtccgagtag tttttcgaga gagattcatc
     1921 acatgatcaa tcgttacccc tagccgttga caaatctgca tcatcaccaa tacattcggc
     1981 agttcctcat tattttcgat ccgacttaat acactttgtg aacaaatatc atgcgaaagc
     2041 attttttgcg tcattttttg ttcttttcga atctctttaa tgatcgttcc aaaggactcg
     2101 attaacgttt ccataagtat cctccccaaa atatgcgtat tcgcattcta ttcgtatttt
     2161 actttaggaa gtcgcaaact tcaagaaaag acttgcaact aaataatata ctttagttat
     2221 ttaagaaaac tccactagaa agagagcctc agtgtgtttg tctgtggccc tcctactgaa
     2281 aaatttggtt tttaaaatca tttttggcga taggttgcta ggaattctct cattttctta
     2341 gctgtttgtt tcaaatcatc cattttagga agataaacga ttcgaaaatg atctggctgg
     2401 ttccaattaa atccaccacc atgtacaagt agaatatgat gttcatgtaa aaagtccaaa
     2461 acgaattttt catcatccaa aatattgaat cgctttgtat caatttttgg gaatatataa
     2521 aaagcagctt ttggttttac ggctgaaaga ccaggaatat cattgattgc attatagata
     2581 tattctcttt gttcatagat ccttccgcct ggcaggagca gctcatcgac actttgataa
     2641 ccgcctagcg cggtttggat aatttgctga gacaatacat tggaacacaa acgcatggag
     2701 gacaacatat tcagcccttc tatatagcct ttgacgtgtg acttatctcc acttaggacc
     2761 atccagccta ctcgaaatcc tgctacacga tgagatttgg atagaccgtt cagcgtaaca
     2821 acgaacaaat caggcgctaa tgtagcaatc ggaatatgtt ctagaccatc catcactaag
     2881 cgatcataga tttcgtcgga ataaataatc aggttatttt gtcgagctac ttcaacgatt
     2941 tcttctaata attcttttgg ataaagtgcc ccagtaggat tattaggatt aatgataaca
     3001 atagcttttg tccgactcgt tatttttgat ttgatatcat caatatcagg ataccattct
     3061 gcctcttcat cgcaaatata atgaacaggt gtgcctccag ctaatgcaat agaagccgtc
     3121 cacaacggat agtccggcat tggaaccagt acttcatctc cattgtccag caacccctgc
     3181 atacacatcg tgatcaattc gctcactccg ttgccggtgt agatatcatt gatcgtcaca
     3241 ttcggaaaat gtttcagttg gcaatattgc tcaattgctt ttctagcaga aaagatcccc
     3301 ttggaatctg agtagccttc tgagtcacgg gcattcatga tcaaatcacg aatgacttcg
     3361 tttggtgctt caaatccaaa aggtgcggga tttcccgtgt tcaattttaa aatactaatg
     3421 ccctcttctt gcatccgctc cgcttcttct agaacgggac cgcgaacatc gtaactgaca
     3481 ccttctaatt tgttggattt ttcaaattct ctcatcactt agccaatccc tcatttcaaa
     3541 aagttagaac tttccaaatt ttttgttatt tactatttca atatcatact ccatcctaga
     3601 aaattttgcc acttatttag gaaaaacgta agtattaaaa aaacacaaac aagcgttgag
     3661 aacttaattg tcatgatagt cattccatag attcgctgcc caatccattg caattccaca
     3721 aatgattcct agcataaaac caagcaaaaa acgtccgaat gcaagtccaa tcaatacacc
     3781 taagatcatt cccagtcctg aaaattctcc gtcatatttc tccatcttaa taagtcctcc
     3841 catttttgag cttgacaact atgtaaaacc tcgctatcat tagtgtgtta ttattacgca
     3901 gatgtgttgg aattggcaga caagcatgat tgagggtcat gtgagcgtta gctcgtgtgg
     3961 gttcgagtcc catcatctgc actacaactc gtgaagttaa ttttgcttca cgagtttttt
     4021 tgtttgaacg tttctttatg ccattatata catatcactg tatcctttac aaaaagaacg
     4081 tacaatcatg taaacagcct gaggtgagta tatgaaggtc ggaaacaagc tactcgtaga
     4141 aacttgcaag tgttaaaaca attaatcgaa aaacgagtaa aatccctcac ctaatactaa
     4201 agcgagggat tttttgtata ttatctgttt atcaccgtta ttataaaggt acaaatacca
     4261 agcacggtta atccaaattg cataaattca tttttttgta cactcatgct tttcattagc
     4321 tctttaactg ccattagtac taagatggcg ataacaacat aaagtatata gtacgtccgc
     4381 attccggaag cccatactgt gggagaaaat cccataatta accttgaaca aaatcccatt
     4441 atcatcaaaa taactaaaaa aatagaagtt ttcttattgt caaaacttaa ataaattcca
     4501 acaattaaac atagtaataa tgccaagact actaaagtag ctatccaagt acctggataa
     4561 aaaagtgaca gtttcgttcc taactctgta aagagattat tcaggttact actactccaa
     4621 atcattgccc ttttatttcc attgacgtaa gtaaagcttt gacccattgt attaccaaaa
     4681 taaataatca ggtttaagaa aaacggtaat gcagtcaata tacgcacata atagttttga
     4741 catttcctat atgttagaag gaaaattaag aaaaatagca ataaaaataa gatattcatg
     4801 tct
//
LOCUS       DACDIH010000123         4790 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.124, whole genome shotgun sequence.
ACCESSION   DACDIH010000123 DACDIH010000000
VERSION     DACDIH010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.124"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..266)
                     /locus_tag="IV281_002670"
     CDS             complement(<1..266)
                     /locus_tag="IV281_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781605.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILG"
     gene            391..1176
                     /locus_tag="IV281_002671"
     CDS             391..1176
                     /locus_tag="IV281_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAP8781606.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            1885..2244
                     /locus_tag="IV281_002672"
                     /pseudo
     CDS             1885..2244
                     /locus_tag="IV281_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2539..3672)
                     /locus_tag="IV281_002673"
     CDS             complement(2539..3672)
                     /locus_tag="IV281_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8781607.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(3663..4331)
                     /locus_tag="IV281_002674"
     CDS             complement(3663..4331)
                     /locus_tag="IV281_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8781608.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
BASE COUNT         1561 a          936 c          778 g         1515 t
ORIGIN      
        1 cccaaaatat caacgattgt ggcaatcagg tcttcttttg attcttcatc tgaaagtaat
       61 tcacgataca acattgtttg tttgcttact gtgtcatatt cctctaagga ttcatctgct
      121 aaaagaacca cttgttccaa caaacggtcg gataaatatt tatagaaaat cagtcccaat
      181 aaataatttt tatattctga cgcatccatt ttactacgga gattatctgc cgcactaaat
      241 aatttttgat ttaattctgc acccaaaaag atacactcct atctcttatt taaagttgct
      301 atataccctc ttattttagc atactccctt ttagaaaaag caaaaaaaaa agaaaaaatc
      361 ttattcttga taacaagcta cgtgacgtat atgcctaaca aaaatagaac ttttgaatca
      421 atcttaaatg aaggtttaac cacagaaccg gcatccatta tttttgatga agttatcgat
      481 gacgttgaag atagtaagta tcataaaaat ttaaaagaac ttttttattt tgcactcaac
      541 aatggaccaa gtttgttcac tggtatatca gtacctgata gtgataatcc attaattatg
      601 gcaaaaattt acttaacaaa atggtgtaat aaatacatca gggataggaa taacccagca
      661 ttaaaaaaac ctcttaaaac atttggcgaa aaagatgctg ctttaactac tagagtagca
      721 gcaaatgcta atattgatga tcaaaaagta ttgaatgact acttgcgtgg acatttttta
      781 tatatgagcg ctgagaacat gaacggaagt attttagagg aatatttagc cgaagtgtta
      841 gaacctgaag gttggatttg gtgcgccggc tctgtatatc gagctgttga tttctgttat
      901 ttaggaacct caccaattct tttacaagtg aaaaataaat acaatacaga aagcagttcg
      961 agttcagcta taagagtagg aacaacaatt agaaaatgga atcgtttaaa taagtcgaca
     1021 aaaatttctg gtttggatag ccctatcccc aattggaaag cattaataga aatgactgaa
     1081 gctagtaagg aactggcagc taagttaact gaaaatagtt atttagctta tattaatgaa
     1141 aaatccacac gagaattatg gacactcgat gactaacaat atccttatta gtatctatcc
     1201 acgatacatg gaaaaaatta taaacaaaca aaaaaacttt gaatttagaa atttctatct
     1261 tacctcagat ccttataata tcttttggat ttacgaaaca atccccacca aagctttaaa
     1321 gtataaattg attgtaaaaa atccaattac taaacttaag aaaaatcaac actacttttt
     1381 aggagacgat gagtttgaac aattagttta aaagggaaaa tatgcttcta tcattacaag
     1441 ctcgtttggt gtaagatggg tataagtcat ttatgttcac tctccttgta tgctttagcg
     1501 ggtattacaa tttgagtgta acataaatgg cttttttatt tgtctcgctt aattatacaa
     1561 acggcgaatt ataaataact cataaaaaat attttgactt tcaaaatcac ttttgctacc
     1621 agaagttgag gcgaagtgtt ttaagcatag ttgtacccta gtttgaccgt taaaaataaa
     1681 taacgatcgc tagagtgcaa ctatgtttgt ttgccctacc gtcagcgtcg gcttggcctc
     1741 tcacatccat taccgtcggg attatctccg ttacataccc ttacaaaata cgccctgtaa
     1801 gcattgcatg aaaccaccgt gcatttgcac cgtatttccg ccctagccgt tcaaatcggt
     1861 cgctaccacc attcagaaac acgtgcggta atggaaggtt tggaggaagc tatccagacc
     1921 actaacgatt gtccctatcg tcgtacgttt cactcagatc aaggttggtc gtatcaaatg
     1981 aaagcttata gaaacaaatt gaaggagtac aaaatctttc aaagcatgtc tcggaaaggg
     2041 aactgcttag ataattcact tatggagaat ttttttggct tattaaaaca agagattttt
     2101 cacgggaaag tttataactg tttcgaggaa ttaaaatcag ccatcgatag ctatatctat
     2161 tactacaata atgaacggat aaaacagaaa ttaaactggc aaagtcccgt ccaatttcgt
     2221 aaaacaaccg ttacggttgt gtaaaaatat cattttagag catgaaaaaa agagtggatt
     2281 tctccactcc aaaaaagtct aacttttggg ggtcactaca tttttgatcc tttttatttg
     2341 attaattata tccagctgga taataatttt gggggcaaca tttctatctt actattatct
     2401 tcctaaagta gtagactttc ttattgggaa aaattacaac aaaaaggagt ggcttagttg
     2461 acaactcctt tttgtttaca aatcaattgg atatctataa attgtatttt ctaacgaatt
     2521 ttatagacat ctgatttgtc aaattaagct agacaatatg tctttatcta tgcagctcaa
     2581 aaatacttcc aatggtgttc gataatttaa tgattttctt ggaatatggt ttcttttcga
     2641 ggcaacggat tgaataaagg tttcatccgt gtccctaaag tccattgttt taggtaagcc
     2701 atcctgcttg agcccatgca ctcggtactg atgagaaaaa attgatgcct gtttctcctg
     2761 aggaatgcca attccctgat cttttacttc aataatcacg ctaccctttt ggtttgaaat
     2821 ggtgacaatt accagttttc cttcaccata tttgattgca ttgtcaacta aattcgaaac
     2881 cgcccgataa atccaacgat ctcgtccatg aatcagggtt ggttgatctt cagtaaacgc
     2941 aaacaaaaga ttcggataat tcattttgta ggcatcacaa acttccgcac agatcagtga
     3001 aacgtctacg acttccgtgc taagatcctc tgagttattg gataatgtca aaatatcgtc
     3061 aatactgtcg gtcatccgat ctaaaagcat taaatttcct tgatcggcat gttgccgttc
     3121 tatttccaga ttcgtacgca aaatagcgat ggcatttttc tgctcgtggg tcagataact
     3181 atttaatcgt cgataatcct ccaaatgacc atcgaactta tcctttagct tattaaaagc
     3241 cgcatttaat gcctcgtcct tcacagtatt tttaggaact tgatcaataa acgttaaatc
     3301 acgcgtaatc gctaagattt gattactgta aattttgcgt aaaaccaacc aaagaataaa
     3361 cgttaataac atgacggcag aacaaataac cagcaccatc gctggtaaat atttaaatag
     3421 ttcgtcctgg taagctttat ctatcatcga aatagtcata aattgattgt tggaagattt
     3481 cgaagcttcc atctgcgtct ctgcttgaac cgtcttaccc tcttccataa aataaacatc
     3541 ttcacctaag gtagatgcga tactgattcc ctccaaatta tttctcagaa agaagaaact
     3601 cactaaaatc acgcctgtca ccaacagtga aaaagccagc atcgttaacg tcattttaaa
     3661 atttaatagc atagctggta cccctttgct cgaatggttt ttaagacctc tttttcagct
     3721 gcttcagcca gtttcttttt tagacgagcc agatgcactc gcagaaccga cgaaaaagga
     3781 tcaaaatctt catcgtagac atgttcagaa atttcctcag acgaaacaac cgctggatga
     3841 cgctgagcga tgcacagtaa gatatcaaat tccttgggtt tcaggaccac ctctgtcttt
     3901 tgatagctga cccttcgacc taatggatca atcttcaaag caccaattga aatctgcggc
     3961 agcgcacgtc catgataacg acgaataacc gcgcgtattc gtgcttttaa ttctagtaac
     4021 tcaaagggtt tgactaaata atcatctgct ccaaaatcca agccttttgc tcgttcttca
     4081 atctcatcac gtgcagtaat aatgataact ggactttcga cttcttcagc acggagactt
     4141 tgtaaaacag ataatccatc ttttttaggc agatttaaat ctagtagtat cccatcgtat
     4201 gaattgatga aggccttctc ttctcctgac tcgccatcat aagctacatc tacagaaata
     4261 ttctcctgct ctaggccctt ttttacggat aaaaccagct cacgattgtc ctcaaccacc
     4321 agtacacgca tgtatattca ctcctcatac ggatttagta attacactta tgctttttag
     4381 catagtacac gctcttcttc aaagtccacg ttctttttgt gaagatttga agaaattcat
     4441 acattctgtt aaaaaacaca taattaagaa tgattttact gtttgaaaat acctagaata
     4501 gaaaaagact ggaacctttc ctccagtctc actactttct atttgcttgg ttttcttgta
     4561 tatacttttc aaagttcgtt ggtttatgac caataagcct cgttagaata ctgaaaattg
     4621 ccatcaagtc attaagtacc acatgtaaga aaatctctct aaagaaaaac cccttcgttt
     4681 taactcactt actattttag aattcgttcc cctaataatg cagcaggttc tgttgcgaag
     4741 tttgaaaatc aaacgcgccc tctctgaact ccaaatatct taggctgtta
//
LOCUS       DACDIH010000124         4595 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.125, whole genome shotgun sequence.
ACCESSION   DACDIH010000124 DACDIH010000000
VERSION     DACDIH010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.125"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..857
                     /locus_tag="IV281_002675"
     CDS             <1..857
                     /locus_tag="IV281_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP8781609.1"
                     /translation="FSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQVGNKA
                     DSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYADLDQLG
                     RAGNVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKDSNNEI
                     TQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSETIVRDA
                     IYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKGININY
                     QNGTWVRD"
     gene            877..1203
                     /locus_tag="IV281_002676"
     CDS             877..1203
                     /locus_tag="IV281_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781610.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDD"
     gene            1237..2499
                     /locus_tag="IV281_002677"
     CDS             1237..2499
                     /locus_tag="IV281_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781611.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVANFSKSVYLVGRALVVSAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATRGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETEKSTASIAVSNSQFAFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            2579..3955
                     /locus_tag="IV281_002678"
     CDS             2579..3955
                     /locus_tag="IV281_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP8781612.1"
                     /translation="MAMKLLEKVKNRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISDTPHMLVAGTTGSGKSVLLNEILVTAICHAMPDELKLW
                     IVDPKRVEFGRYKKLPYMLADPITDMDEAYEFFEYLVILMNERYKLMEKAGVQNITQY
                     NKYAEKHGIEKFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMFIKLNGGNKIRAQG
                     AYISNEEIEAIFDHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQAEKLEKVLKALDK
                     VDPEPQTSNNTNNEQPISSKTKPKTTDPKVSVKVTITTDNSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            3952..4581
                     /locus_tag="IV281_002679"
     CDS             3952..4581
                     /locus_tag="IV281_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781613.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKKDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNERKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
BASE COUNT         1764 a          680 c          951 g         1200 t
ORIGIN      
        1 agtttagtcg atcgttgtta ggaattttgg ttttagttgc ggttggtctt agtggcaagg
       61 gttactttca gaataacagc actcaggaac atgattttca gatcaaaagt agtgttcagg
      121 taggtaataa agcagacagt gaatcaaaag aatttttagg gatacgttat gatccaaaca
      181 aatatccaaa taatatcgta aacattaatg atgggaaata tacactgaca aaagaagacc
      241 atgaagcatt atcagaaaaa ggcagtcaaa ccttttgggt aaattatgca gatttagatc
      301 aacttggaag agcaggaaat gttgaagcgt tgatcaccta tgatgctgta aattctcact
      361 catctaaaaa tgtggaacgt ccatctttca aaagttctgt ccatattgca ggagaataca
      421 aggatggagt atttgatcaa gagagagata cttgggtcgg caaagattca aacaatgaaa
      481 tcactcagct agaaggctat aaaggttatc tctataacaa aagtcattca ttagcatggt
      541 cgctaggagg agatatggag gtacacaatt taacacttgg aacgagagcg caaaatgttg
      601 ggaaaaatga tcagaatgga gggatggctt actcagaaac gattgtacgt gatgcaatct
      661 ataaaaataa gaatgaaaaa gtattgtata aagttgatcc aatttataaa gggaatgaat
      721 tggttcctcg tggttctcat gtcgaagctt tttcagttaa cgataatgga aaaacggtaa
      781 accttaatgt ttatgtattt aacaatcaaa aaggaatcaa catcaactat caaaatggta
      841 cttgggtaag agattagaaa ttgagaaggt gaagaattgg aaacaataag catcaatgga
      901 ttagataaat cagttgcaaa tcgaataaaa aaaggaaatg gctactatct tctgaagaag
      961 ggtaaatatt cagttgctag agagttagaa acaatgagta tcgtgtatgt agctaagaat
     1021 cattttgacg tagaacaggc tatttttgat ccagattctg aaatagggaa atgtgttgaa
     1081 ttatcagaga aaactgttag tacaaaaggg gcgatgatca ttgagagaga aatgttagac
     1141 gaaatcagag taatcattcc taaagatacg cagatgtgtg aggacagcat aagtgacgat
     1201 taaccaaaat cagaaagaaa ataaggagat attcagatga gcgaaaaatc cattgaatta
     1261 aaggaatata agcgaagcga tggagaaaaa tattatcctg tagccaattt ttctaaatca
     1321 gtatatctgg ttggacgtgc attggtagtt agtgcaattg gcatatatat tttgcattgg
     1381 gtaacaaagg ttgtacaact gcttgtagat accttagtaa gtattctcgt ctcgttaaat
     1441 gatgttaacg gaacagatga tttaggatta aaagagatgt ttaaagagtt actcaatgcg
     1501 ttttttccaa attatttaag ttattcatat aatgtacaaa gcttttttgc aaagctacac
     1561 ttggtagata tctctatttt agtattagaa gcaggaacgg tattctttgt cggatatttg
     1621 gttactttga tgttcaggca tcactatgga gaacaaagac cattctttga tgatatggaa
     1681 agtagaaggt tgaaaagaga agctctagaa gctatggatg tgaagtatga tccaaacaag
     1741 attataggag atgaaacgaa caacaaaaaa agaaaacgaa acaaaacgca attagaatcc
     1801 gatgtgctat caacgcttaa aaatatcaaa atttctgttc atacaagacg tgatctaagt
     1861 gtcaatgaag atattcgaga atatatcgta aaaataccgc aaccaaaagg gaagaaagtt
     1921 agagaaacag ttttacgaca aattcaagat ttagggtcga tcctgacaag agcaacaaga
     1981 gggcgagcta aatttgggga aaaagaagac gaagtttcag gtgattactt catcttcaga
     2041 gcatcagtaa acattgaaag agaacgtaag gaaagagtaa gtaccaaaaa caaggaaacg
     2101 gaaaaatcaa cagcatccat tgcagtttca aattcacaat ttgcttttcc aatcaatctc
     2161 tttgcagata actccaaaaa tatccaaaac caaaccgaaa aagctagaaa atatgccgaa
     2221 actcaaaagc aatctttaga actttatcta tcttctgctg acgtacctgc aagtctaaca
     2281 gatatgcacg ttgggaatac atctattgaa tatttgttta ctttatctcg taccagaaaa
     2341 attcaactaa gctcaatgaa agaagaaatt gaaacttact tgcatatatc aggtgtaatc
     2401 gtgcggatca acgcagggcg tattcaaatt actttaccat taggagaaaa tgatcgtatt
     2461 ccaattgatg taccatcaat gatcaatagc gtgttttaga agacatcaca aatagaaaca
     2521 gaaaatacaa actaccagtt cacaaacaga aaagtaccga gaaaagagag gaatagaaat
     2581 ggctatgaaa ttgttagaga aagtcaaaaa tagagccgaa aaagctgaat ctattttgct
     2641 agataaagaa cctaatataa agcttaatgg aaaattcagt gtcaatatgt tgaacacaaa
     2701 ggctaaagac ccgacagatg ctatttttgg aattaagcct gatggcacaa ttgctcattt
     2761 ttgcatcagc gatacgccac acatgttagt tgccggaaca acaggatcag ggaaatcggt
     2821 attactgaat gaaattcttg ttacagctat ctgtcatgca atgcctgatg aattaaagct
     2881 atggatagta gacccaaaac gagtagagtt tgggcgttat aaaaaattac cctatatgct
     2941 tgctgatcca attactgata tggatgaagc ttatgaattt tttgaatacc ttgtgatctt
     3001 aatgaatgaa cgctacaaat tgatggaaaa agcaggcgtt caaaatatca cacaatataa
     3061 caaatacgct gaaaaacatg gaatagagaa atttccctat gtgatcttat tggttgatga
     3121 atattcgcaa ttagttggaa cacacaaaga agttgaaggg ctgatcgttc aattaggaca
     3181 aatggcaagg gcggcaggaa ttcacgttat tcttgcaaca caatcacctc gatctacagt
     3241 cgtgacaggt attattaaag caaatttccc atctagagtc tgcttgatgg tagcaagtga
     3301 tttagaaagt aggatcgtat tagatgaggg tggtggagaa agcctaagtc caaagggaga
     3361 tatgtttatc aagttgaatg gaggaaacaa aatcagagcg caaggagcct atatcagcaa
     3421 cgaggaaatt gaagcgatct ttgatcacct tagaaatact atgccagaac cagaatatgt
     3481 tgattaccga gcaatcgtag ctgaatctcg tggtgagggc ggtgaagacg aggattttgg
     3541 tggaattggt aaagctcctg taagaaaatc tgctaaaaga aatttgaaca cacctttagc
     3601 aaattcatca tctaaagagc catcaattga aaagaagagt acgtttgctc taaaacttga
     3661 aaaaaacaaa gaagaaaaag aaaaacaagg agctaaaaca ctagaaaaac ttcaagccga
     3721 aaaattagaa aaagttttaa aagcattaga taaagttgat cctgaaccgc aaacctcaaa
     3781 caacacgaat aatgaacagc cgatttcatc taaaactaaa ccgaaaacta ctgatccgaa
     3841 agtttcagta aaagtgacca tcacaaccga taattctgag aagaaacaag taaacggaac
     3901 tacttcacct atcatgcaaa aaatgttaaa agccaaaaaa caggtgcata aatgatcatt
     3961 gtcagttctt ttttgaggtg gcttacgcca tataaatata cagatgaagt agttgtgaaa
     4021 cgagctaaga aaaaagataa aaaagcggtg ttaactccta aacagatgct tgatcatgga
     4081 attttgggtg tgaaggtttt agaagtgaat aaaccgtttg aatcaaatag tgcaagagaa
     4141 aagttgcttg gcgctgaagc agatgtggca tatttttatc atgacaaaag caagcatcaa
     4201 atagctatta aattcaatcc aaaatcggca tggcggtatc aagcaagaga tgacttgaaa
     4261 cgtaggaaac ttagaccaat gctctatcca gaaggaaaag aagcatttga tagaacccac
     4321 atcgttccca tcggttttca tggtacagaa tcagataatc gcctattagt aggttggaac
     4381 agcaatcaaa atagagttca gatcaataaa tttgagaaga aagtaagaaa attcaacgaa
     4441 cgaaaaacaa tcctatggtt tgcagatatt tgtaaacagc cggacggaac agcaaagtgg
     4501 gaagcggtga tatttgacga aaatggtaaa aaagtaatgt ctgaaacatg gcatgataaa
     4561 gctaaatttg tttggcagta gaggtgaaaa ttgtg
//
LOCUS       DACDIH010000125         4465 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDIH010000125 DACDIH010000000
VERSION     DACDIH010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.126"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            574..1008
                     /locus_tag="IV281_002680"
     CDS             574..1008
                     /locus_tag="IV281_002680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781614.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="IV281_002681"
     CDS             2298..3221
                     /locus_tag="IV281_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8781615.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="IV281_002682"
     CDS             3237..3776
                     /locus_tag="IV281_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8781616.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1667 a          674 c          757 g         1367 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacggagaa cttaaattaa attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagagacg gataaataat aaaaaagact
      181 gctctttcga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tatgaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacacccca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttga cttagaccaa tcgaaaatca caaagtgagc ccctgtatcc cttggtagag
      421 aggctacttg gcatagtaca cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct ctcaacgtgg taaaaatccc acttataaga gaaaatggtc aagaacctaa
     4261 acggtcggcg aagaaaacat accaagcacc acaagcaaaa aaagttagag taacttccaa
     4321 aaaagaaaaa ttttttagaa caacttttga aaatttaatt ttgatttata tccgttcaat
     4381 ttaaacattt gataaactga aggctagtat ttaaaatgtg tatacaagtg gtagcgttaa
     4441 aaaaagtgga ggtataacct atgtt
//
LOCUS       DACDIH010000126         4393 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.127, whole genome shotgun sequence.
ACCESSION   DACDIH010000126 DACDIH010000000
VERSION     DACDIH010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.127"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(606..962)
                     /locus_tag="IV281_002683"
     CDS             complement(606..962)
                     /locus_tag="IV281_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP8781617.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(976..1386)
                     /locus_tag="IV281_002684"
     CDS             complement(976..1386)
                     /locus_tag="IV281_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8781618.1"
                     /translation="MIKVYTSPSCTSCRKAKAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1732..3918)
                     /locus_tag="IV281_002685"
     CDS             complement(1732..3918)
                     /locus_tag="IV281_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8781619.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            4136..>4393
                     /locus_tag="IV281_002686"
     CDS             4136..>4393
                     /locus_tag="IV281_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP8781620.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKH"
BASE COUNT         1222 a          822 c          696 g         1653 t
ORIGIN      
        1 tgtttgttgt cttgaattga atcaagacgc cctacacatt tggtttgtct tactttgttc
       61 agttttcaaa ggtctacttc atcaggagtg ttccccgatt cgtttttggc tgccgtagca
      121 acttttatat catatcagag agacaagtaa ttgtcaactc ttttttaaaa gtttttttga
      181 tttcgttttt cgaaatctta cagcaactca tttatcataa cttatcattt gttatttgtc
      241 aagaactttt ttgaagtttt tttcgataga atcaagcttc acaagtggtt cttttttaac
      301 aactcagtta tcttatcatg tcatttctaa catgtcaaca actattttat atgtttttta
      361 aaatactatt ttattgcttt gctacttaaa acaacagaaa tgaatatatc atgtaaattt
      421 acacatgtca atatattttt aactctatat ccccacctag atttgaattt tgaccacata
      481 aattgagcat gtctctatag tagaaaaata aagtcaaaaa aagaacacat aacacgcttg
      541 atgctaagtg ttcgactgtt acaactgtat gaaatgctca gtgacttgat taaaacagtt
      601 aaagtctatt tatttaagat aagctgttcc atcaactgct cattgtatcc aataaataga
      661 acttcatcga taataatcaa aggacgtctg aggagattgg tatgttgtgt gcataaaaaa
      721 atcagttgtt tttttcctac acaggaaagg tcaatcgcaa tgttgtgaaa agcctcagac
      781 caagtcgcaa atagctgttc tgcttgcact tcttcaaaac tgcataattc ttctgcttct
      841 tctgtactta gatcgcgttc ttctaagtca acaaattgat aaggcaattg atggtgttct
      901 aaccattgtt tcgccttcag acttgttaca cagtgcgctg atccatagac tttcacttcc
      961 acgaaggctc cctccctatg aatttaagat gttattgttt tgtaaataga tcagatttct
     1021 ggcaacacat cgaatttcac gagggacaaa tgaccggatc tcatcttcat taaatccgat
     1081 tagtattctt ttttcatcaa tcaaaatagg tcgtctcaat aatgaaggat attctttgaa
     1141 taagacgatc atttgcttta aagttaattc gttaaaatct atgcccagct tttcgtaaat
     1201 tttcgaccga gtggaaataa tatcttgaac tcctccttca gtcgctgcca agatcttttt
     1261 gatctcactt tctgttaacg gttcggcaaa aatattcttc tcttcaaatt caagttgatt
     1321 cgcttgcaac catgcttttg cttttctaca ggaagtacag ctaggcgaag tatatacttt
     1381 tatcattttc tcactccttt ttaattataa tccaattcta cagtaatttc ttttaaaata
     1441 ccaattatta gctaataaaa tataaaaaaa atcaaaataa ctgattattt acgtttgaat
     1501 aatgagaatt tttctatata ttctctattt tgataatagt attctcattt tcaaaataaa
     1561 taagggaggt gtgacaaaat tcttgtcaca cctccctttt ccgaataaac tgtgttccag
     1621 aaatagcttc ttcgaaaata agctgaaatt tcacaaaaat ttgagaagca attttcgtac
     1681 attccttctt ctttcttgaa actgaccact tctgtcacac cctctcttct atcactttcc
     1741 ttttttcaca cgactcccgg tttctttgat aaatctcgat actttcaatt ttcttgaatt
     1801 tttcatttga ggtacacata gatacagacg atttcttgct ctagtcatcg caacataaaa
     1861 tagtcttcgt tcttcttcta tcgtcgttac tggttctgct tttatttcat taaagtcgat
     1921 tactttcttt tctgtcgctc cgttataagg caaacttcct tcgttacaac caatcaaaaa
     1981 gacatttcgg taactaagac ctttactttt gtgaatgctc tggatacata gtgcatcaag
     2041 ttttccttgt ctgtaagcaa tcaagcgggt ttttaattct ttagctgtcg cttgggtttc
     2101 ttgcgataag gcttggagtc ttctgaaagt agcatgctgc tttacttctt ctcgtatcca
     2161 ttcttgatca ctctggtcga tttccttagc tatacgtggc agatgaagat agctttgaac
     2221 aagcgaatag atttcttgat gctggtctgt tttacgcaac gcttcctcaa actctcggat
     2281 ttctgcagat ttcgatttta aattggggct tatcgacaaa atataatctg caacggatat
     2341 attttgatat ttaggagaaa agtattgatc gcattcactt tttttcaggt agcagtctgc
     2401 catcagctgc cggagcaatt gctggtaggc ctgatttttc ttttgcagat tcagtgctgt
     2461 ttgccaatga acaatttttt gatagaaatg ttggaaagac ttgataccaa agataccttc
     2521 atatggacta tctgcttcca gtacaaaagg aatattcgtt ttcagcagag attggacaag
     2581 ctgctgcgtt tgtgaagctg atcggtataa tatgattgat tccttatatt tcccattttt
     2641 ttgaataaat gtctgaattt gtttgattag ccactgactt tctgctttag tatcagaaaa
     2701 caaataggct tccataacac ctgcttcttt ttgtgctgga atcatcgcct ttggtatccg
     2761 atcattttta ttatagctga tcaatctatt tgctccttgg acaatagctg cccgagaacg
     2821 ataattagtt aataagaaaa gagttttggt aggatattct ttttcaaagt tcttgatgta
     2881 tgctggctct gatcctctga atccatagat actctgatca tcatctccaa caacaatcaa
     2941 cttgatcctg ttttcctttg tcatccgttt cacgatttcc cattgaagtg gattagtatc
     3001 ttgaaattca tcaatgaata gataatcata tttttggccg atataatgct ggatctcctc
     3061 attctccaaa gcgcttttca gattttggag tacatcattg aactctagca attgctggct
     3121 tcttttttgt tcttggagtt ccgtgagcaa tccatacagt cgttctgata tcggctgtcg
     3181 tgcattctta tcaatggaat catatttttc tttaactaac tgaaccattt cttctactga
     3241 aaggttcata ttctgccaat agtctaaccg agaagcaatc gttttttctc catattgatc
     3301 aaatatataa agattatatt tacgtatcag ctgttgcaaa acagcattca ctttttgtgc
     3361 agtatccttt gaaaagtctc cttgtattcc ttgcattgct acttcaggaa attttcgaca
     3421 aatttcattg tatatcctaa aaaagatact atggaatgtt ccgatatgga cagcggtttt
     3481 tctctggctg aaattatcta ctcgtttttt tagttcattt gcagcgttcc ttgtaaaagt
     3541 gatcattagt atgcgttctt cgtctacttg tttttgcgtc atcagataaa cagcttttgc
     3601 acagattgtt tgcgttttgc cgctcccagc tcctgctatg attcgaagat gtttcttcat
     3661 atcatatgtg atagctgctt tttgttcttg attgaagcga ttataggaac gtttctccat
     3721 cattttaaag aaagagctgt ttccttcact ttcttttact tgtacttctt cctcaataag
     3781 tccgggtgct tcgccataag ccgcttcata ggtagcaatg atttcttctt gtagccgaaa
     3841 gacttgttga agctttttat acttgttctc ccagcgtttc aattcttttt gtaatcggag
     3901 aatttctttt tcattcaact gattgatttc cttcccatct ttcttctttg aatttgttta
     3961 tttcaagttg gttatttaaa gtttcgttgt ctattttttc tttatactaa cagacctttc
     4021 taaaaatgaa aatgggaagc gatatgattt tagttttctg aaaatttttc ctttttttca
     4081 atcgtttatg ctaagataag gaagacaaaa atattcataa aaggtgatga aaaaaatgca
     4141 gacttccttt cacgaggggc gtacaatagg acttttgctg acatttatcg gaggagccat
     4201 ggacagttat acttatatcc aatatggtgc ttttgcctct gctcaaactg gcaatatcat
     4261 tctatcgatc atccaagcat ttgacggaga atggctaagt gttggtaaaa aagtattgtc
     4321 tacccttttc tttttcttag ggattcttct aacaaagtat ctaattgatt attttagaaa
     4381 aaaagaaaaa cat
//
LOCUS       DACDIH010000127         4372 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDIH010000127 DACDIH010000000
VERSION     DACDIH010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.128"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(83..1051)
                     /locus_tag="IV281_002687"
     CDS             complement(83..1051)
                     /locus_tag="IV281_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP8781621.1"
                     /translation="MSKKRTIVNEWEKLTALSNRRLNVQTLVQTIKLNWSIDRITIVG
                     KLNQNIFYHTQHDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQHDENIAYIEISKW
                     QEGKGRIDFNPNKISRFLADSMKNFIHDLFLEPHFSRADVACDLIDVPDEFITQYRII
                     DPVSFKLIYGRSGKLETVYWGSRASERQIRLYNKKIEQEMKRKVVPSEINTWWRLEMQ
                     LRRGKATEWYAMVKESLDSFVSPHFLPTDIKPTDKVMIDGLLANHSNWAILSRNSKYK
                     YRNLLKRESQNDELTNHLRETFEESADDLKKELDTWLQGLDVTEEE"
     gene            complement(1496..2902)
                     /locus_tag="IV281_002688"
     CDS             complement(1496..2902)
                     /locus_tag="IV281_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP8781622.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSENEIEW"
     gene            complement(3124..3486)
                     /locus_tag="IV281_002689"
     CDS             complement(3124..3486)
                     /locus_tag="IV281_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781623.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKTVDNEWLADEKSAPSERDYPDESDDLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3490..3798)
                     /locus_tag="IV281_002690"
     CDS             complement(3490..3798)
                     /locus_tag="IV281_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781624.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3807..4226)
                     /locus_tag="IV281_002691"
     CDS             complement(3807..4226)
                     /locus_tag="IV281_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781625.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4219..4359)
                     /locus_tag="IV281_002692"
     CDS             complement(4219..4359)
                     /locus_tag="IV281_002692"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781626.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1241 a          979 c          604 g         1548 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tactattcct cctctgtcac atcaagtccc tgtaaccaag
      121 tatccaattc tttttttaaa tcatctgctg attcttcaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcgcttta ataaatttcg atatttatat ttcgagtttc
      241 ttgacaaaat cgcccaatta gaatgattag ctaacaatcc atcaatcata actttatccg
      301 taggttttat atcagttggt aaaaaatgtg gactcacaaa gctatccaaa ctttctttca
      361 ccatagcata ccattccgta gctttaccac gtcttaactg catttctaac cgccaccaag
      421 tattaatttc tgaaggcacc acctttcttt tcatttcttg ctctattttt ttattgtaca
      481 aacgaatttg ccgctcgctt gcccgactac cccaatacac cgtttccagt tttcctgagc
      541 gtccataaat taatttaaat gaaactggat caataattcg atattgcgtg ataaactcat
      601 caggcacatc aataagatcg caggcaacat ccgctcgtga aaaatggggc tctaaaaata
      661 aatcatgaat aaaatttttc atggaatccg ctaaaaatcg actgatttta ttaggattaa
      721 aatcaatccg cccttttcct tcttgccatt tagaaatttc aatataggca atattttcgt
      781 catgttggtc taatagctgc caaccattat ttccaaccgc ttttaaatag ccattgcctt
      841 catttacccg cataagctgt tcaaaatcta atattaatac atcatgttga gtatgataaa
      901 aaatattttg attcaacttt ccaacaatgg taattcggtc aatcgaccaa tttaatttta
      961 tagtttgtac caaagtttga acatttaacc gcctgttaga cagggctgtt aatttttccc
     1021 actcattcac gattgttctt tttttactca tattcagttc ctcctcaaag aaaaaagaga
     1081 tttttggaaa gaatttccag agttcacgta actctaaaaa ttcataacca tcaatctcta
     1141 gtcttgaaga aacctaaaaa tattcgtata atgaatttga gttacggaac tagtgtggtt
     1201 agtttccgta aaggtcgaga acgttggtag cgttgctcga cttatttttt tgctcttttt
     1261 ccagttcatt tgtttgctaa ctcaataata gcacttctct ttagctatgc aaataccaaa
     1321 aaaataaaac cgcacccttt gaaaagggcg cccccaaact tcgctaacgc taagttggcc
     1381 aaaaaatgga cttgccattt tttagcaaat gtcagcttca ggggctagtg gtttagccgt
     1441 caactcctta tcctcggcac aaaccgcctc cggtaccgtt gactgcacat cgtggctacc
     1501 actcaatctc attctcagaa cgctctaatt tatgctgtga gacgttttga cgataaaata
     1561 aggttttctc ctgctctaat acttctaatt cctcagaaag ccccatatcc ttttctgcaa
     1621 gaccaaaacg gatagacaaa cgagtgatcg atttttgaaa tgcgtttttt aatcgctcta
     1681 aacgctggcg taaagagcga ttttcttctt tcagcttttt attttccact tgtaaatctg
     1741 ctaattgttt tctaccagac aaatgacgct cttcttccaa tgtagctaca tactcattta
     1801 attctgtatt ttgctcaagc atctgtaaat attgctcact catgttatca gcatttctct
     1861 tggcatttgt tgctctcgta gctcgttgat acaaatcaac aaaatcattc ccagataaat
     1921 gatacgaacc atcaggaagc ctcttgaaac tttctaaagg aaccatatct ttttggaaaa
     1981 tgaccgccat ctcttttttt acattcagca tatttttctt ctcttcttca atcttcgctc
     2041 tctcagaagc catcagagcc ttatcacgct cgttttcttc tctttgccta taaatatacc
     2101 tcaactcatc agaaacgctc tcacgctctt ttacagccgt ttggcgcaat tcagaaattt
     2161 cactcatgat ttctttgtat tcgtgaatat ctttaatttt gttggtaccg acaatatccc
     2221 ttttccaacc gtaatcattc attaatcgtt ctatagagtc aacttcttga tttctaaaat
     2281 tattgaaaag cactcgacca ttatctttat cagcgtgtat cccttgttga accagtgctt
     2341 tattaaagct cggctgcttt tgaacacccc gtttataacc ttcagcaacc ggcacaatat
     2401 tcaagtgcag gtgcggactt atttcgtcat tatgaataac agcattataa acagccaaat
     2461 tagggtttct ctcttgaaac tccttgtaat actgaaccaa aattttattg gcaatcactc
     2521 gattttgttc agcttgttgt ttcgatatgc cagtagaaga accatctgct cgatttgtct
     2581 ggaaatcatc taaaatgcct atttgcacaa taaattctcg ttgaggttcc aactttttat
     2641 ccttgcgcac tttcgctaaa tagtcctcga tttttctatc ggctcttttt tgtttcgcat
     2701 tatatttttc taccacttcg ccaaacaatt cctcatattt atctgctaga ttcgtctgct
     2761 ctaaataaat attttcatcc gatttcgtga agtcaatgtg cttatgccaa tcattggttt
     2821 tctggcgatc agttaactca cgattattat gagaaatact tgtattcttc gttccttttt
     2881 taaatgaaat actaaacccc aagttaaaac cctccttcgc gctgtcgcta ataaaaagat
     2941 tgtaaacctt tctgttactc tttgtcaaag agtaacattt tcctaattgt gacatggatg
     3001 tcacaattcc aaacgctctc cgagggtttt cgctgtcaca agtgacaacg aaaaaaggag
     3061 ccgaaagcgt atcacgctat caactccttt tatgctttat tttgcttcac gcaaaataat
     3121 cgcttatcgt aaattcacgt cattaaatat tgacgtaaat ttactctcac tcatctgact
     3181 tttcttattc ggtatcactc cgaataagtc gtcagattca tcgggataat ctcgttcgct
     3241 tggcgctgat ttttcatcag caagccactc attatccact gttttttcgt gttgctgatc
     3301 gtcagaaatt gacgataacc accgctcaaa atcagcaaaa gtttcaaacc caaaacgctt
     3361 gcgaagagcc ttgcccattg cagcattttc tttctcaatc aagcgttttt tctgttggcg
     3421 aagtttcgct tgttcttttt ttaatttttc ttcttgcgct ttaatatctg ctaaagtcat
     3481 tcccataaac tacatctcct tttctttgga attaataacg gaaatttggt ttaactgcat
     3541 aacatgactc attaatttca accaaaattc tttttctgat tcatcagttg tttgcgctaa
     3601 atgcttatga acaaattcat tagtcaaaac tgaagatttt gctagatcag cattgataac
     3661 cgagttgaga acatccataa ttcgttcaag catttcacta taattatctt catccggcaa
     3721 attcaaataa tcagctaacc gttcatacat caattcaatt ctatcgactg tttcttctct
     3781 caaatcaatt ttatccatgt cacacctcac ttccagaatt gccaccattt tcgatttttt
     3841 aattcttgat tttctagtaa caactgatcg tttttttgtt gctgaagcaa agtcaatttt
     3901 tgttggtcac gaatcatttc cgttaattca gaaatatgcc tatctttttc cgctaattgt
     3961 tcacgcaaaa attcattttc tttttctaac gggaaatcat tgcctgcatc attgaccggt
     4021 aaatcaccag tcaattgacc accttttgac cgataaattg accgcacaac accaatcaat
     4081 tccttgtcta acatgataac cccattatct ttataccgag caaggtcata acctgcattg
     4141 accagttgat tgcgcactgt atttttagat atacccaact catcagcaag gttttttatg
     4201 gtcatttttt ctggcaaatc agtcattatc ttctccgtta ctttcagcta ccccataaac
     4261 atatttaaca gcatgcttag ggataatgat tggggaatca attttattat ttttcaaaat
     4321 caactcatac tgcgttacat caagtaactt accggacatt tttttgctat cc
//
LOCUS       DACDIH010000128         4271 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.129, whole genome shotgun sequence.
ACCESSION   DACDIH010000128 DACDIH010000000
VERSION     DACDIH010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.129"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            204..1478
                     /locus_tag="IV281_002693"
     CDS             204..1478
                     /locus_tag="IV281_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8781627.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            1532..2959
                     /locus_tag="IV281_002694"
     CDS             1532..2959
                     /locus_tag="IV281_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP8781628.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDRLYMKPVRELEQLRVG
                     SGVETTVNLAGEIIIAQDASFKEALIDVLLTKSKNQEVKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            2972..3844
                     /locus_tag="IV281_002695"
     CDS             2972..3844
                     /locus_tag="IV281_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8781629.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNKLLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            3939..>4271
                     /locus_tag="IV281_002696"
     CDS             3939..>4271
                     /locus_tag="IV281_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8781630.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQ"
BASE COUNT         1443 a          587 c          852 g         1389 t
ORIGIN      
        1 taaaaaatag tgccattaaa aaagaaaagc gacacaccaa taaaagtcat atcctgtgac
       61 attatatggc attatccaaa tagaataacc ggttgacatg taagcggatt tcttttataa
      121 ttttaggagt acaagtcctt ttctaaaaat gttaaccggt tatcatgaaa ggggatacaa
      181 atatatcgtt ggaggataaa aatatgtcaa aagttaacgt atttaaaaat caatcttatt
      241 tacaaagttc agctacatta ttactatttt ttgcttcttg gggtgtttgg tggtcatttt
      301 ttcaactttg gctaacatct gaatcaaatg gtttagggtt atctggcagt gctgtaggaa
      361 cagtattctc ggcaaattcg ttagttacct taattttgat gtttatttat ggaacattac
      421 aagataaatt gtatattaaa cgaaatttat taatttttgc ttctgtatta gcgacacttg
      481 ttggaccatt ttttatatgg atatatgggc cattgctaga taacaatttt aatttaggca
      541 ttattatggg agcgctattt ttgtcagctg gatatttagc ttctgtagga gtttttgaag
      601 ctgtgtcaga aaggtttagt cgtttatttg gctttgaata tggacaagca agggcgtggg
      661 gatcatttgg ttatgccttg gtagcgcttt tggcaggatt tttatttgta aaaaatcctc
      721 atttaaactt ttgggcggga tctttctttg gttctttact attgttaaat ttattatttt
      781 ggaaccctaa agttgaacgg gaagcaaatc aaaattttaa tcaagaacaa gctgaatcaa
      841 atagtattcc ttctttaaaa gaaatgtttg atctaatgaa actgcctcaa ttatggacga
      901 taatcatctt tattgttttt acatggacat tttatacggt attcgatcaa caaatgtttc
      961 cgggatttta tactggtttg ttttcaacat cagctaatgg tgaaaaaata tatgggacat
     1021 tgaatgctat tcaagtattt tgtgaagcgt taatgatggg aattgttcca atcattatga
     1081 gaaaattagg ggttcgaaat actttgttat taggtgtaac cattatgtgt gtacgaattg
     1141 gattgtgcgg gtttgcctcg acaccattat ctgtttcatg cataaaaatg ttgcatgctt
     1201 tagaagtacc attatttaca ttaccaatgt ttcgctattt tacacttcat tttgatacaa
     1261 agctatcagc aaccctctat atgataggat ttcagatagc tgctcaaatt gggcaagtga
     1321 ttttatcaac accattggga atattaagag acaacgttgg ctatcaacca acatttaaaa
     1381 ttatttctct tattgtatta ctagcaggca tatatgcatt ctttattctt aaacaagatg
     1441 atagagatgt tcaaggggat ccatttattc gaggataatg gaaaccaaca gaataaaaaa
     1501 caattttatc tagaaagtga gcgaatatat tatgacaaaa agagctatag aattaacaaa
     1561 tacacgttat cgtttgggat atcatgtgtc tgcaccatca ggatggatta atgatccaaa
     1621 tggattttgc tattttgatg ggtattatca tgtgttttat caacatcatc catatagtgc
     1681 tgaatgggga ccaatgcact gggcacatgc tagaagtaag gatttagttc attgggaatc
     1741 tttaccactt gctttgactc ctggagatca agaagatgaa ggtggttgtt tttcaggaag
     1801 cgctattgaa aaaaatggtg tgttgtatct gttctataca ggacatcact attatggaga
     1861 caatgatcca gatcattttt ggcaaaatca aaacatggcg tatagtacgg atggcattca
     1921 ctttactaaa tatgaaaaaa atcctgtaat agcaaaagca ccagaagata acacacatca
     1981 tttcagagat ccaaaagtat ggaaacacga tgaaaaatat tacatggttt tgggtagtca
     2041 agaaaaagat ggattaggtc gtgctattgt ttatcagtcg ttagatttat taaattggga
     2101 ttacattgga ccaatttcta aagcgaagga agtacttaca gaaggattta tgtgggaatg
     2161 cccagacttt tttgaattaa atggaaaatc tattctttta ttatcccctc agggaattga
     2221 agcaaatgga aaagattatt taaatctata tgaaacaggc tactttatcg gtgattatga
     2281 ttatgagaca gcaaaattca ttagaggaaa tttttatgaa ttagataagg gacacgattt
     2341 ctatgcaaca caaacaactt tatctccaga tggtagacgt attgttatgg cctggatgga
     2401 tatgtgggaa agccctatgc ctgaaaaagt agacggatgg gcaggagcat taacgcttcc
     2461 tcgagaatta gagttaattg gagatcgttt gtatatgaaa cctgtgagag aattagaaca
     2521 acttcgagtt ggcagcggcg ttgaaacgac agttaatttg gcaggagaaa taataatcgc
     2581 tcaagatgct tcattcaaag aagctctaat tgatgtacta ttgactaaat caaaaaatca
     2641 agaagtcaaa ttttcattaa aaacaaaaga tgaagagtta atcaatattt tttattctaa
     2701 ggataaaaat gaatgtacct taactcgtaa cggcaaaaat gatccaaggt atggaaccat
     2761 tcagatgtgt gatagattat cacttcgagt gtttattgat aaaagttcgg cagagatttt
     2821 tattaatgat ggcgagcttg tttttacaga gcgttattat acagaaaatc aaccaagtat
     2881 gcatttgagt gttagtgaag aagcttttgt ctcgagtaca atttatcatc taaaaaaaga
     2941 tgcaatttct tacgagtaag gaggttttat gatgttaatt ggtagcatag aagcaggtgg
     3001 gactaaattt gtatgtgccg tgggagacga ggattaccga gtacaagacc aaataatttg
     3061 tccaacgacc acccccaggg aaacattgaa aaaaactgtt gactatttta aacagtttga
     3121 tataaatgca ttaggtattg catcatttgg tccaatagaa attcgaaaaa attcaccaaa
     3181 atcaggatat attacgtcta caccaaaggt gggatggcga aatgttaatg tgttaggtta
     3241 tttacaagat gagctgaatg ttccaatgtc ttggacaacg gatgtgaacg gatcagctta
     3301 tggagagtac atcatgtcca ctttatcgaa tgaaaaaata aattcattag tttattacac
     3361 cgttggtact ggcgttggtg gtggagctgt tgttgacgga aaactattag gatcgtttgg
     3421 tcattcagaa atgggccata cgtttgtaaa acgccatcct gatgatattg attttaaagg
     3481 aatttgtcct tatcatggtg attgtttaga aggcttagtt gcgggaccaa cgtttgaagc
     3541 acgttttgga aaaaagggtg aagacgtttc tttaacggat cctatttggg atgtaatggc
     3601 ctattatcta gcacaagctg tgattcaaac aacattgatt atacgaccgg ataaaatagt
     3661 atttggtgga agcgtagtaa atgaaaaatt gttagaaaaa attcgtgtta atgttaataa
     3721 attattggat gattacgtgg aaataccacc gttaagtaaa tatattacgc gtcctttaat
     3781 taaaaataat ggatcggcaa cactaggaaa ctttgctttg ggcttgaatc aattacaaga
     3841 ataaaacata gaaaaaatat cgattatcag gtatagtagt tctatagagt tctttttttc
     3901 gatagttaat tattgcatta tttaggagag acaagagaat gcgcccaaaa ttagaagatg
     3961 ttgccaaaag agcaaatgta tcaaaaacaa ctgtttcacg tgtattaaac aatcgtggct
     4021 atttaagtca aaaaacgatt gataatgttt ataaggctat agaagaactc aattatcaac
     4081 cgaacgttgt agcgcgtcaa ttatttcaaa agaaaactaa cattgtcggg ttactatttc
     4141 ctacagtagc caatcctttt tttagtgaac tcgttgaggc attggaaaaa aagttatatg
     4201 aaattggtta taaggtatta ataggcaatt cgatgaataa taaagaaaag gaaacaaatt
     4261 atttaaatca g
//
LOCUS       DACDIH010000129         4240 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDIH010000129 DACDIH010000000
VERSION     DACDIH010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.130"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(3..1451)
                     /locus_tag="IV281_002697"
     CDS             complement(3..1451)
                     /locus_tag="IV281_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8781631.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(1451..2167)
                     /locus_tag="IV281_002698"
     CDS             complement(1451..2167)
                     /locus_tag="IV281_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8781632.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(2148..2498)
                     /locus_tag="IV281_002699"
     CDS             complement(2148..2498)
                     /locus_tag="IV281_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP8781633.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2642..3415
                     /locus_tag="IV281_002700"
     CDS             2642..3415
                     /locus_tag="IV281_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8781634.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            3586..3952
                     /gene="ssrA"
                     /locus_tag="IV281_002701"
     tmRNA           3586..3952
                     /gene="ssrA"
                     /locus_tag="IV281_002701"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1253 a          836 c          752 g         1399 t
ORIGIN      
        1 ttttactctt ttttgatttc tacgtcaggc aaacgaacag cgaattttgt tccttttcct
       61 agttcgcttt ctacttctaa ttttccttta tgcaagcgta caagttgctg cacgattggt
      121 aaacctaatc ctgattcacc atattttgta ttttttcttg aaggatctac tttataataa
      181 cgatcccaga tacttttcat ttgttgttca gacattccaa ttcccgtatc ttcaatcgtg
      241 atgatcgttt caagatagcc tttttctaaa gtaatcgtga tttggccgtt ttcagtaaac
      301 tgtatcgcgt tttgaataat attgaccatg atttggacaa atcggtcata atcagcgtaa
      361 acatcaatat catcaacggc tttcaatgtc aatgtatctc ctgctgcttc tgctttagct
      421 tctaactgtg caacaatatt tttcaatgtt tctgttccat tgaatttttt aatgaccata
      481 ctgatttgat tcgtacggat tttttcataa tccaagtttt cattcaccaa acggatcaga
      541 cgctctgttt catttttcat caatttaatc gcgttttctc gctgattttc agggattgca
      601 ttgtattcta acccttccag aagtccgttg attgtcgtaa gaggtgttct catctcgtga
      661 gaagcatctg ccataaattg tcttcgacga ttttcttgtt cttcaatttc tgcttgagat
      721 tctttcaatg aattcgtcat tttattgaaa tcatctgcta actcatcaaa ttcatctttg
      781 tcatgtacgg ggagctgtac atcaaaatta ccattcgtca cttctttcgt cgcattcctt
      841 aaccgattga tacgcttaac ttggaatgct gcaaaagcat agctggcaat aactgcaacg
      901 atactggaaa aaataaatcc tttgaacagg ttcagtgtaa ttggacgaac gctgttatcg
      961 atatttcgcg caggttgact aactacaagt gctccataga attcatgatt caaattaaat
     1021 ggcacaagtg cgtaagaggt cgcctgtgct tgtcctaaaa tatttttatt cgatgtgaat
     1081 ttttcttgac gtccattcct cagatttttc cattgatcag ctgttaaggt aaaattcagt
     1141 aaagctgtat tagtcggata aatgacacgt tcgtttttat cgataaatat aaagttcaca
     1201 ttttgttctg tcagtacttg ctctgtcagc agcaatgcat tttgaaacgc ctgatcttga
     1261 ctaaactgcg ggagcgtatc ggcataggtt tgcgttgtct tttcaactga ttcagcatat
     1321 ccaaacagtt gctggtaatt gttttcttca atcgtttgtt ttgtcagttg tgtaaaagaa
     1381 actccaacga tcaataagat caacgcgacc attccgcaaa aagctaagag ttgctgatac
     1441 aaataacgca ttaggcaaca ccagaatcat cgaatttgta gccgactccc caaacagttt
     1501 ggatgacttg tggtccgacc ttttcgattt tttgtcgtaa tttcttgata tgggcgtcca
     1561 ctgtccgttc atcaccaaag tactgataat cccagactaa ttcaagcaac tgctcccgag
     1621 agaatacttg acgtggtttt tttgctaaag taaacaatag atcgaactct ttaggcgtca
     1681 gtccttcaat tggccggttg tccagatacg cttctcgtgt tttcgtattc atcttgaaat
     1741 gactagtctc gatatcaaaa tgatcatctg aaactgctgc ttcttctctt gcttctgcca
     1801 actcactacg gcgatgcaaa gctttcattc gagcaataag cgtcagagga ctgaaaggtt
     1861 ttgtgacata atcatctgcc cccatctcta agccgatcac ctgatcgctt tcagaatctc
     1921 tagctgtcag catgataatc ggcacagtac ttgaaacttt gcggatctct cttcctacta
     1981 gcataccgtc cagtgtgggc aaattcaggt ctagtgtaat catatcccaa tgttgtgggt
     2041 tttctaaaaa ggcatcgagc ccttcttttc catcatactt gaaagttgct tcccaacctt
     2101 catttaagaa aaacatctgc atcatttcag aaacagattg attatcttca atcattaaaa
     2161 tattcattat cgttccctcc agtttccagg tcatatttct gtaatttctt ttctgtcata
     2221 ataaaaatag gcataattgg aaatactagc agaagcattt caatcagaaa ataattcgta
     2281 tgcccagatg tctttaaata atagccgatc aatgccatca gtaagcccat aagaaaaaac
     2341 cagtttccgc ttgtcttttg agccagccgc caatgagcgt ctgtttttgc ggcaagaggc
     2401 gaatgatacc cataaaaagg cagatttcct tttgagggca gaatccgaaa aagactgcca
     2461 attattaaga ataagatacc aatactgaaa aaaatcattc tttccctcct aaattctctt
     2521 cttatagtat actctaaatt gttgactgaa ccaatatgtt caggaattct tcacgaaagg
     2581 tttgtctttt gttcattctt tgttaagata aacttattcc aataaaaaag gagttttatt
     2641 tgtgtcaaat tatcgtgcaa tcactttctt tgatcttgat ggaacattac tagatgcgaa
     2701 atcaaaaatc acacccgaag tggcaaaagc tatgaacgca ttaaaagaaa acaatgttct
     2761 ccccgtcatc gctacaggac gtacagaagc agaaatccac tctatcatgg aagatgcggg
     2821 cattacttct gcggtcacca tgaatggttc tttcattcga gtagacggcg aagaaatcta
     2881 ttctcaaaca ttttcttcag aagaatgtca aaaaatgtat gatcatgtac agttgaacgg
     2941 tcatgaactt tcttactata acgatcagaa aatctggtgt accggtcata gccaaacaat
     3001 gatcaatgct tacgacttca ttcattcaga tgttccggaa attgatcctt taggctttaa
     3061 agaaaacaca gtaaatatgt tgctgatctt atctcaatca ggtgacgaat actatcacga
     3121 acattttcct gagctaactt tttatcggaa tggtccttac tcgatcgata cagtgaagaa
     3181 aggaatctca aaaggggcag gtgtcaaacg tctgcaaaaa gaacttcagc tggaaaaagt
     3241 tcctactttt ggatttggcg atggtccgaa tgattttgcc ttgttggaag cttgcgacaa
     3301 caaaatcgct atggacaacg cctatgacgg attaaaagaa ctttctacct ttatcacaaa
     3361 gaaaaataca gaaggcggaa ttgtccacgc attgaagtat tttgatctga tttaagatct
     3421 ttttttgaag ctacctcttt tcttaagaaa atcgcttatt ttttcttaag aaaggggcag
     3481 ttattatata aataatggga tatgattcgc ttttttattg aatttatccc aatatatgct
     3541 atactaattt tgtatgaaaa atacacgtaa cccttgttag ctaacgggga cgttacggat
     3601 tcgacaggca cagtcgagct tgaattgcgt ttcgtaggtt acgtctacgt aaaaacgtta
     3661 cagttaaata taactgctaa aaacgaaaac aactcttacg ctttagctgc ctaaaaacag
     3721 ttagcgtaga tcctctcggc atcgcccatg tgctcgagta agggtcctaa ctttagtggg
     3781 atacgtttca actttccgtc tgtaagttga aaaagagaac atcagactag cgatacagaa
     3841 tgcctgtcac tcggcaagct gtaaagtgaa tccttaaatg agttgactat gaacgtagat
     3901 ttttaagtgg cgatgtgttt ggacgcgggt tcgactcccg ccgtctccat atatgtatgg
     3961 ttgagacaga taaacatgta tgaaaaacgt tgttaaatca acgttttttt attttggtca
     4021 aggagagaaa agtagagaaa tacaaaattt tttgcattga ttttgcattc aaaaaaacat
     4081 tcaaacattt agaaatcccc aatacgtatt caggaaaatc tattttaagg tttacaaaaa
     4141 gaacttttgt tcgtacactc tttttgaggt gaacttttat gctgatggaa ggaaaaacac
     4201 aattatggtt taagcatgtt ccttcaaata agttttttta
//
LOCUS       DACDIH010000130         4074 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.131, whole genome shotgun sequence.
ACCESSION   DACDIH010000130 DACDIH010000000
VERSION     DACDIH010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.131"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..391
                     /locus_tag="IV281_002702"
     CDS             <1..391
                     /locus_tag="IV281_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HAP8781635.1"
                     /translation="AGDSFAEAYYYGMCILEEMQWIKTRPVHAADFFHGTLELIEAGV
                     SVIITEGEDDSRPLTERVKNFVPKFTDVHVTVDTKDYDLPGISEDTRKIISPVVLATV
                     FERISAFLEEERNHPLTTRRYYKQMEY"
     gene            499..1368
                     /locus_tag="IV281_002703"
     CDS             499..1368
                     /locus_tag="IV281_002703"
                     /inference="COORDINATES: protein motif:HMM:NF012585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781636.1"
                     /translation="MKIAVICSGGDSPGMNSALLAIKKTIDSEPDSEVVFFKNGYAGI
                     FKECIASVSLKEVEVFKAGTLIGTARFEKMRDLESRKIIIENLNKMEIDNLIVIGGNG
                     SLKGAELLKNESELQVSVIPGTIDNDFPNTDYTLGFDSALNTIIRSIDDLTSTANAHD
                     RIIIMEVMGRGCFALPEYASIANNTYWVERDDKEYLHLICKKFESEFYSPILLLPEGI
                     SNKQKIIEFITKNLPKETKYLNLAYLQRGSSPTFYDRYIGYLWGQLAAKELIEGKSGI
                     LSQKNGNIMLIPW"
     gene            1383..2087
                     /locus_tag="IV281_002704"
     CDS             1383..2087
                     /locus_tag="IV281_002704"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012610.1,HMM:NF019322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8781637.1"
                     /translation="MIFDKVKEQIRKVYITTDRKSELLPSERDLAQEFSVSRPTIRKA
                     LASLEDEGIISKNEGRGYLVKPASEKYVDHELDSFIGFFQDAVQQGKKISSKIIQQTI
                     IPADSTISECLEIEQNTLVLVLERIRYIDDYPTCIAKSCIPLSVLPEIVEHDFAKESL
                     FSVLESRGIRLAYAKRSIEVTPPTDLENLYLDLEKNEPVFVFTSIGFAKNQKPFEYEI
                     SKYPAYKTKFESTVSK"
     gene            3308..3921
                     /locus_tag="IV281_002705"
                     /pseudo
     CDS             3308..3921
                     /locus_tag="IV281_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406632.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT         1378 a          703 c          709 g         1284 t
ORIGIN      
        1 tgctggtgat agctttgcgg aagcatatta ctatggcatg tgtattttag aagagatgca
       61 atggattaag actcgtcctg ttcatgcagc agacttcttc catggaactc ttgaattaat
      121 tgaagcgggt gtaagtgtca tcattacaga aggagaagat gattctcgac ctctcacaga
      181 acgcgtgaag aatttcgttc ctaagtttac agatgttcac gtaacggttg atacaaagga
      241 ctatgactta cctggcattt cggaagacac gcgaaaaatt atttccccag tcgtcttagc
      301 tactgtcttt gaacgaatta gtgcttttct agaagaagaa cgtaaccacc ctttaactac
      361 tcgccgttat tataagcaaa tggaatacta atcataaaaa tatgaaaaag gtgaccgcga
      421 ctccattgcc catttgattg cttcattttc tctaatggga gaatagaatc gcagtcacct
      481 tttttaggag gatctaaaat gaaaatcgca gttatctgta gtggtggcga ctctcccgga
      541 atgaattcag ctttattagc aataaaaaaa acaattgata gcgaaccaga ttcggaagta
      601 gtctttttta agaatggtta tgccggaata tttaaggaat gtattgcctc agtttctcta
      661 aaagaagttg aggtattcaa agctggaaca cttattggaa cagccagatt tgagaaaatg
      721 agagatctcg aaagtcggaa aattattatt gaaaacctaa ataagatgga gatcgacaac
      781 ttaattgtta ttggaggtaa cggttccttg aaaggagctg aactattgaa aaatgaatca
      841 gaattacaag tatctgtcat cccagggact attgataacg atttccccaa taccgattat
      901 acacttggct tcgatagcgc cttaaacact ataatccgat caatcgatga tttaacttct
      961 actgctaatg ctcatgatcg tattattata atggaagtca tgggccgagg ctgctttgct
     1021 cttccagagt acgcttccat tgcaaataat acttattggg ttgagagaga cgacaaggaa
     1081 tacttacact taatatgtaa aaaatttgag tcggagttct actcgcctat attgctttta
     1141 ccagaaggta tatctaacaa acaaaaaatc attgaattta ttaccaaaaa tttaccaaaa
     1201 gaaaccaaat acttaaatct tgcgtatctt cagcgtggat catctccaac tttttatgac
     1261 cgatatattg gctatttgtg gggtcaatta gcggcgaagg aattaattga aggaaaatct
     1321 gggatattaa gtcaaaaaaa tggtaatata atgttaatac cttggtaaaa ggatggatta
     1381 ctatgatttt tgacaaagta aaagaacaaa ttcgaaaagt gtatatcacc actgatcgga
     1441 aatctgaatt attgccttcg gaaagagatt tggcccagga attttcggtg agtcgtccaa
     1501 caattcggaa agctttagct tctttagaag acgaaggaat tatttctaaa aatgaaggtc
     1561 gcgggtatct agtgaaacca gcttcagaaa aatatgttga ccacgaactc gactctttta
     1621 tcggattctt ccaagatgcg gttcagcaag gaaagaaaat cagttctaag attatccaac
     1681 aaaccatcat acctgctgat tcaacaatta gtgaatgttt ggagattgag cagaatactt
     1741 tagtgcttgt cttggaaaga attcgataca tcgatgacta tccaacttgt attgcaaaaa
     1801 gttgcattcc cctatctgtt ttacctgaaa ttgttgaaca tgattttgcc aaagaatccc
     1861 tgttctcagt attagaaagt aggggaatta gattagctta tgccaaacgc tcaattgaag
     1921 ttacaccgcc aacagattta gaaaaccttt atcttgattt agaaaaaaat gagccagtct
     1981 ttgtctttac aagtattgga tttgctaaaa atcagaaacc gtttgaatat gaaatatcta
     2041 agtaccctgc ttataagaca aagtttgaaa gtactgtttc caaataattt ttaaatatgc
     2101 ttaaatgtat aaagagcctc gcggataaca gataaagtgt ctttccgcaa ggctcttttt
     2161 ctacttcctc tttatcttaa tccgctaagt agcctacagt ccaaacatta tcatctaatt
     2221 aaggtgtgtg gaggtactcc tctaccctaa gtcaaggata gtcgacatag ttgttaataa
     2281 gtcttcaaag caagcctgaa cctttttcat taggtgtgct tacttcacat atgacgctat
     2341 caaaaatgat agcacccgtt gaatcaacca attttctcta attcatatcc ccattcaaac
     2401 aactatattt taaaaacttt tttaatcttt gattatgtgt agcaaatttg tctaattttt
     2461 atttctttgc aaaatttttc caaatttacc agataaaagt aattcatatt catgattgag
     2521 tcctttacca ataaaaccta tttcgcaaaa attatttgta agcattaagg attttctgct
     2581 gactttcact ggaaggataa cccaagaatt ctgaccaaag tgaaagatta agaaccatct
     2641 ttgccagtct atctatgtgc tattgtaact gtcaacatca tgcgggcagt tattattatt
     2701 tatttgaata atatttttat tataaatcag ttttttagat tgtgcataat cactaattgc
     2761 tgtgattaat tacagaatat ggctctctgg gaaaatgtgc gcgtcatgta ttcaatttta
     2821 aattctaatt tgtacaaaat gaatcgtcac gactcgatct gacgcaactc caaatggggg
     2881 cgccgacctg taccaagtac agtggcatgt tctttaaaca tttgaagcaa aatctgtcca
     2941 tcaaaaacct gtattttcaa agtgaacaag gtgcgctaaa tcaggaaata cttacactga
     3001 tcatttctta atttacctac ttaaccaaaa tttaaatcaa tatgaaagca acacttttca
     3061 aactaaagag gtctttttac cgcttgatgt ttgcttcgat aaaaaagttg atgtcggatt
     3121 agttttcatt ttaataaaac gtactattcc tgaaaaaaat ttatccacta ctaagtattc
     3181 ggatttttct aatttttaac aaaatttatt tcgaaatttt ctgatattat attcgcaaca
     3241 ttagtgaaaa ttcttagata aattcattct ttcaatctcc gaattttaga tatatgtgaa
     3301 atatctgttt tttgaatgtt tgcataagtt aaaagatgag cgtgctttag tattctttag
     3361 aatagcttct tatacagggg aacgaaaagg agaaatactc ggactgacat gtagagacat
     3421 agatttcgat aatcaaacta tctgcttcaa caaaacgtta gtagaactat ccgagggctc
     3481 tctatgtatt aatcctacaa aaacagacaa atcaaaggga gtaatttcaa ttgataatgg
     3541 aaccttacgt attctaaaaa atggaaacgt tctgtaacat aaaagaacct ttctagaggg
     3601 attcggatag ataatataga tgatatacct gtaatcagca ctatcaaagg gaataaaaaa
     3661 agggacttcc tttacaaagg ctaccctaac catgtcatgg ataaagtagc acggaagttc
     3721 ccagatatga agataatcaa agtccatggc ttcagaaaga ctcatgctac ccttctcttt
     3781 gaagcaggtg ctcaagtaaa agatgtacaa gataggctag gacattcttc ttctaaaaca
     3841 atcatggaca tttatattaa agtatcaaaa aatcggcaaa atgaaacagt cgaattattt
     3901 gattcttaca tgaacggatg ataagcgagg tcaaaaacgg agtcagagga tattatgggg
     3961 ataccttttt acctcttaat cctttaatat caatccttta agcttcgact tttgcaatct
     4021 tcccttgttc gatatagacc caaatcgatc tatcaataat tatcaaactg agtc
//
LOCUS       DACDIH010000131         3989 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDIH010000131 DACDIH010000000
VERSION     DACDIH010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.132"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(76..708)
                     /locus_tag="IV281_002706"
                     /pseudo
     CDS             complement(76..708)
                     /locus_tag="IV281_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
     gene            complement(721..2697)
                     /locus_tag="IV281_002707"
     CDS             complement(721..2697)
                     /locus_tag="IV281_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8781638.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            complement(2750..3421)
                     /locus_tag="IV281_002708"
     CDS             complement(2750..3421)
                     /locus_tag="IV281_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8781639.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(3481..3690)
                     /locus_tag="IV281_002709"
     CDS             complement(3481..3690)
                     /locus_tag="IV281_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781640.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            complement(3757..3966)
                     /locus_tag="IV281_002710"
     CDS             complement(3757..3966)
                     /locus_tag="IV281_002710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781641.1"
                     /translation="MDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEKERLRKEFDYW
                     RTHNGIKDLKPARPVIKRKKKITQD"
BASE COUNT         1067 a          855 c          596 g         1471 t
ORIGIN      
        1 gcaaaaaaaa gcgaatagca gtgctaatcg ccaatccaat gaaattgatt tcctattttt
       61 tgcttttttc tgtagtttta aatcttcttt actgaaggaa actcgttcac catacactaa
      121 aaagcggtgt gagttgacac cttttggtgt gcatgtcatt aacgtgatcc tatcttggtc
      181 tttttcaggc ataagataat ctaaataatt tttttgtaat tcttctgctt taacttcttc
      241 aattcgataa atttgatagg catgttgttc ttcatttacc ttaataaaga atttttgccc
      301 tttttttact gaaggcaaat catcgaaaag attatcttta taaagtccac tgtgccccgc
      361 gattaaagga tttttaccgt ttcctcctgt aatatcgcca gttccttccg taatccctac
      421 accattctct aaaattttgt cggaagttcc gtcataaatt ggtagtttta aagaaatact
      481 tggaattgta agaacaccaa caggatcgcc taactctatt ttcgcaacat ccaaagaaga
      541 atctgttttt tcaagatttt caatggctaa aattctttgc tgttgttgaa atttcgctac
      601 atttgtttcc acaactggaa ttaaatagac tgctactgcc gacagcaacc aaaaaaatat
      661 catcagtttg tgcatgactc ttctttttgg gggcttacga ataatcatta gctgatcctt
      721 ctaggctttc ttcttaaagt aaacaactgc agaaagtccc cctgctccta gcaacaagca
      781 agagatcaat acgtaagatt ggaaaccgtt gctaccagtc attggtacgg aagctttttg
      841 attatttttg atcgtgattc ggttttcttc tttgtatgaa tctttgtcta ctttgaaaga
      901 gatctctttt gttaattttt gatagttttc aggagctttt gtttcttcca gtgagtaatc
      961 tccttcagtt aaaccagtga tttcaaaatg accctcttta tctgatttta ggattgctgc
     1021 atttgctaca tcgtctaccc aaactgcttc tttgacacca tctttatctg tatcagtaag
     1081 tccaccgtag tacttacctt ctgcattctt agctgcaaat tctgcgcctt caagcggtgt
     1141 tgctttgata cctgttccaa ataggccgct atcgactttt aagaattttg cgccataagt
     1201 cacaacgtca gctttcgttt tttcgtggtg ttcttcctga ttaaatggat tattgttata
     1261 aacgaaatcc acgctatttg ttaatggatt tgcgatttca gcactatcat tcacaaacat
     1321 gttataagtc atttttagat cttgaccatg taaattcgca actgtatcac ttacttgtcc
     1381 attatttacg ataaaatcga tcacaaaacc actatcagaa ttttcgattt tatagtcagt
     1441 attttctttt aatacgactg aaccatcatt atttgttact ttaatatctg ctttttcagc
     1501 aacataagaa agtcctttac ctgctgtatc tccccattta aatacactgt aaggagaagt
     1561 ttcgcttttc acaccgttta ctgtgatacc gtctgcgcca cctgcaatgt ctgttggaat
     1621 atgaatcgtt ccttgataat ttactgcgtc accaatttgg aaagaatgtt cttgtcctgt
     1681 tccatttgtt actgttttat cgatcactgg tgctttatag tttacgtatg aagcttttaa
     1741 gtcacttgtc acgccatcat tcgtaatttc aaaggttaat ttattgtgcg catcgttgcg
     1801 agcgtctgcg cctaataaat attgatctgc tgttggttct tgatctgatc ccactacaac
     1861 ttccgaagga acttccacaa aatagtattt tcctaacgtc aaatcagtcg cagtcagctt
     1921 tccttgatca tccgatacaa gatcacccaa ttttgttcct tttccaggtt cgcccttgta
     1981 taactcaaat tttgcccctt ttaaaagggt ttgtttgctt tggtctgcgc catcgtcacc
     2041 aaatttcgtt aattcaaaac tcaacttaga aacaatgttc ttaggataca aatgaatatt
     2101 ttttaaaaat cctgatcctg ttttatctgt cattggcgca atcacaacca ttggttttgc
     2161 tttttgtgtg actaatccag cagcggattt actttcataa accacatata cattcccttt
     2221 ggaattgaca taggctggtt gatcagtaaa agtgatctca ccattttcat ctacagctga
     2281 agtaattttg ttggttgcat tcttgacgta ctcactgtta gatcccttgt cttctacatc
     2341 ttgtacaatc tcgtccactt tcgcgttggt tagatcagaa tcttctctcg gtaatacttg
     2401 atccgtaata tttcctaacg taaactcaac atcaccataa tctgccttgt tgtatttcgt
     2461 tacacctgta ggatattgat cgtgcgtata accatcgttt ttgatcccgt caatatcaac
     2521 attcaattgc gttccttgat cgtacattaa tttatgaacc gtgatattga ccttctctgg
     2581 tggtgttttt tgcgtgacat cttctgcatg tacttgatac ccctgaaagc agcctgcaat
     2641 gattcctaac attccaacaa gcattccagc tttttttaca taccgattta ttgccatttt
     2701 ttactctcct catttcatat ttcaacgact cgcgttgatt aaaaccttgt tattgtagct
     2761 ctccctgtac cacgtatctt tcttttcctt gtgacgtata aacacaagta aacaacgtta
     2821 ataagggttt ctctcctttc tttggcaaag acaagaaaga gttctcttca ggaacgccat
     2881 cgattaattt aaagtaccca ggtacgataa atttacgcgt taccttgtaa cgatagacgc
     2941 catcgttact aactaagtcc atcacctccc ctaatgacaa cttttgaata tcagaaaata
     3001 aaacacctgg catttccatg ttatgacctg ctaggacata gtttcctttg cccatttcgg
     3061 catcttcata aaaataagta gccgctccaa gactcaaagt gtattgattt gcccctttaa
     3121 aaattggcaa tgctaaacca agtgcaggaa tctgaatttg tgcaaatact tgtttattga
     3181 cgcccatttc gattgcttct ttttgtatgg acaacatttg ccccaaagac atatcattat
     3241 tgacgttcgt tgcatcctcc ttataactct ttgcatgtgc attgatttga tctacgggca
     3301 tctttttttc tatattttgc ttaaacacct ctccttcatg tttttggtag ctaacatagc
     3361 caaacacact gcaaaagacg attaaagtga ctcctaaaaa accaaataac cattttttca
     3421 tccatctgcc tcctttcaga caaaacaaaa aaactttttc tcatttccat agaaaaagtt
     3481 ttaaaaatga attctataag ttcgtctcaa ctgttcaaag tttttcattg aacctaacaa
     3541 acaagctttc tcttttcctg ttaactgcat atcattgata tattgcgcga cagataatct
     3601 tttaaggtgt ttttgcttca tcgtttttag taacttttct tccatttctt tcgacacttt
     3661 ttttctgttt tcgatttctt cataattcat gagttttcct accttccttg ttttttgcaa
     3721 aaataaaaaa gcctatgatc acatacgctc tacacttcag tcttgggtta tctttttctt
     3781 tcgtttaatt actggccgtg ctggtttcaa atccttgatt ccattatggg ttctccaata
     3841 atcgaattct tttcgcaatc tttctttttc catatctgtt cctttagata aggctttgaa
     3901 tacttcttta ggtgaataac cacgagctaa cagctgtaca taccttcctt ttgtcaaagt
     3961 atccatatcc atcgcctcta aaactggtc
//
LOCUS       DACDIH010000132         3956 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.133, whole genome shotgun sequence.
ACCESSION   DACDIH010000132 DACDIH010000000
VERSION     DACDIH010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.133"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            105..443
                     /locus_tag="IV281_002711"
     CDS             105..443
                     /locus_tag="IV281_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781642.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            553..774
                     /locus_tag="IV281_002712"
     CDS             553..774
                     /locus_tag="IV281_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781643.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            794..2950
                     /locus_tag="IV281_002713"
     CDS             794..2950
                     /locus_tag="IV281_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781644.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            2962..3195
                     /locus_tag="IV281_002714"
     CDS             2962..3195
                     /locus_tag="IV281_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8781645.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            3185..>3956
                     /locus_tag="IV281_002715"
     CDS             3185..>3956
                     /locus_tag="IV281_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781646.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERL"
BASE COUNT         1528 a          577 c          770 g         1081 t
ORIGIN      
        1 atatcgagac gtctttttct tagttagatc attgacaagt gaacaaataa gatttgtcga
       61 tgatataatt aacttattaa aaatattaaa aaggaggttt ttatatgttc gattctatta
      121 aaaatttggt tagcggaatg acgcctgata tgaaaacttt agtacaagtt gttttgtatt
      181 tcttggcatt cgttttttcg ttgaaaccag gattcactgc aatgaaattt tttggtgatg
      241 caaaatgggg agatggatta aaatatgttg gggcgattat tgcaatcttt attattcctg
      301 taattacaac cgttatgctt tacacaatgg gtactaaaac aggtaatgat ttgaaccaaa
      361 aagctaatat catatcagca cttattccag ttgttggtat ctatctagtt tcaaaatttc
      421 caaaaaatga actgttaggt taaaagtgaa ttgaacaaat ttggataata tgccgatttg
      481 ttcaatttac tttttaccaa aaaactacgc tccaagcgta gtttagaagt gagggaagaa
      541 agtaaatgta agatggtttt tgatagagaa aaaatgttag ctcacgccaa cgaagtatta
      601 atgagcagtc tgaaaggtac cgaactggca aagataatga atatgaatgt taaccagttc
      661 tatgattatc gaaatgggag taaaaagatt gaaaaagcca gacttgaaac gcttattaaa
      721 ttcgaaaaag catatgtata tatgctagat aaacagaaaa gaactattga ctgaaaaaag
      781 gagttttaca ataatggcaa aattcaaaga tattaccgaa tctattaatt atgtggcaga
      841 aacagttcct gtagttgcac tctctttgaa gccacaatta ctcgaagtgt acaaaaaaga
      901 agaaaaagaa gcgaaggcaa aaaaagctaa agcaaaggta tggaaagaat tcgatgcgtt
      961 gctgaccaaa gatttaccaa agaaaaatta tatggtaatt gaagatgtac aaaagctcaa
     1021 cagtgaggga gttatagatg aggatgatgc tattctcgat tctgagaagt atgtcgttct
     1081 attgaagtca agtttgaatt atgtagctga tctaccagaa aaatttaaag agaagctatt
     1141 agcaggtgtc cgttttatgg atagtagtgg tcaattatta gttattccgg atgatcctaa
     1201 tgtgtttgaa tcagaagttg aagaagctac agacttctat agcgcagtag agcaattaga
     1261 agtagctact aatggattag aacgaattgc tgagaccttc aactcttctg aagaagttga
     1321 atttgaagcc gtagcagaaa atgtaccaga tgaaaaagaa gctctttttg tggatgataa
     1381 caaaccaacc gaagcagaaa accaagaagt aagcatagaa gaaacacaaa tacaaacaga
     1441 aacctaccaa caaccaaaaa ttgatgaaaa acccgaatcc caaagtgaca aagaaaatta
     1501 tgtttatgaa gctgaaaaaa tcacatctga aagtgatgta ccgaaaaact tagatgctga
     1561 ttttttagaa aaaaattttg aacagatcat tgctgacaaa tttcaacctg tagttttaga
     1621 tgagattaat gaccctgtcg atttaaacga ggagatgcta tccatacctg agttgaaacc
     1681 attgctgttt ttagctaaag agagtatgaa cgctcgcatc tctattgcta ataataaaat
     1741 caacagtgaa ttaaataaga cgaaggagca attgatcctc aaaggaaagc aaatgttagc
     1801 tgatgagctt ttgcggatag aggaagcaac ctcattaaat ggtcaaaatc agtttacaaa
     1861 tacactatcg gttgctaaag agagctacga agcagaagca tctaaaattg atgatttagt
     1921 gatggaaaaa tccaatatca ttcgtaaaga ttatgatatg gcgaaacagg cttatttgga
     1981 tgctgaacta aagaaactag agcaacggta tgatgctgag catttaccag aagttaaaca
     2041 acaagaagat gaatatagaa aagcaactga agaaagtctt gatcaacagt accaagaagc
     2101 aattgaatct attaaaaacg atgcattgaa acaaagccaa gaagcaaaag aagttgtggt
     2161 ggaacgaatt atcaattcat tgaaaccaga aattgaaaaa tcattaacag acgtgcaaga
     2221 aaaaacttta gagattgtcg aaaaaacaaa gtcagaaaat gaagctgagt tccatgatac
     2281 aaaagagaaa acaacagatg tggttgtcaa atattttgcg aacaaagaag aagttgaaaa
     2341 gcaggtagct aacaacacga aagaattaga aacaaaactt gtagagcaac aaaaaactat
     2401 cgaagaatat aaagatcgaa tgaaagaggt caaagattta gcagagggtt ataaattaaa
     2461 tagcgaaaag ttggcagaac aaagtgaagc tttaagagaa gacttatctc gttcccgaag
     2521 tgatctagct caaatggcag aaaaaacgaa atcattagca gttgctacag aaaatcaatc
     2581 aagttcaaaa cgtgatttta caagtaaaaa gatggctact atcatttctg tcgctgcaat
     2641 ggcttgcgga acgatcttgg gatcgtccta ctttattagt cataataatc aacaagcaca
     2701 ggctcaaact caaacatcta tttcgagagt ggaaaattcg attgggacaa atacgaactc
     2761 tgcttcaaca aatagtagct atgaagcaac accaactaca gagttcaaag ttgggaaaga
     2821 tgttgtcatc agtattgatg ggaaaacaac taaggctgaa gtgatttcta ttgatggaga
     2881 aaaagcagtt gtgaaaactc cagacggaaa tcagtacaaa attcaagctc acttatctga
     2941 taataactaa ggagggctat tatgcgagaa gaaaaaatag tttatccagt aattcaaaac
     3001 aatcaagaaa acatgacctc ttctcagcac ttacaggaag cacagctcgt gtatcaggta
     3061 aatgaactac cacaaacgaa cgagacaatg agcaagtcaa tatctttaat tggcttgctt
     3121 attgttgcaa tagtgataac tgtaggtgtt taccgtaagt tgaacgtgaa aggaagtatt
     3181 tctgatggca gataactcaa actatggatt taaacataac ttacatcaaa aaccttctga
     3241 aacaattgtc agagttgaat ggcttgaaaa gcatgtcaat agcacctatt ctgttacagc
     3301 aaaaagatta attgtatcag ctatagttgt tgcagtcgtt gttcttggat caattgttta
     3361 cttagtttct gatacgatta aactgaaatt tatttttgtt ccaacgttat taatgataat
     3421 tggatttctt attctaatta gccagtatat gtttattgat ccatctagtg gttcattgat
     3481 ttctggttct attagactgt tcaaatcagt agttaattca gcaagagtat ctatggggaa
     3541 aaagaaacca tttcgaggca cagagatttt aaaagtgaaa caagatggaa caatcgtata
     3601 tcgagatggg agtgtagcat taatgttcaa tctagatggg gctacatcac taaccgcttt
     3661 tccagaagaa gttatggctc aggaatcgaa atcaatacaa tatcaaaata ccagagatcg
     3721 ttccttagct gaaatcaaaa tcacttcatc tcaacctcag aatacggaaa gacaaactcg
     3781 aaatatgaaa atacaaaaac gaaatgcaag gagtaacaaa gcagtctttg acataataga
     3841 tcagcaagaa cgatttgtat cagaatatat tgatggtgtg aaaacaacag taatccaata
     3901 tcttatcttg aaatctcaat ctaagatggt attggatgaa ggtgtggaac gtttag
//
LOCUS       DACDIH010000133         3734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.134, whole genome shotgun sequence.
ACCESSION   DACDIH010000133 DACDIH010000000
VERSION     DACDIH010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.134"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(268..1566)
                     /gene="eno"
                     /locus_tag="IV281_002716"
     CDS             complement(268..1566)
                     /gene="eno"
                     /locus_tag="IV281_002716"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP8781647.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(1682..2437)
                     /locus_tag="IV281_002717"
     CDS             complement(1682..2437)
                     /locus_tag="IV281_002717"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP8781648.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(2541..3731)
                     /locus_tag="IV281_002718"
     CDS             complement(2541..3731)
                     /locus_tag="IV281_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP8781649.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
BASE COUNT          944 a          782 c          700 g         1308 t
ORIGIN      
        1 tatttatacg agaggacttg cccatagatt cctattctac tgttctatcc ttaaaagctc
       61 ttatatgatg ctagttattg ttagctttcc tttttcaagg atgatctttt cttgtaccat
      121 tctttgtaaa catcatatac agaagctagt tgcaaaaaaa ctctctacct gctgatattt
      181 cagcaagtag agagttcgat tacttaacta agtgatcata ctcgactatc tttcaatagt
      241 cgatcatgta tcttcattag aaaagaatta tttgtttttt aagttgtaga aagatttcaa
      301 acctttgtat tctgcaactt caccaagttg gtcttcgata cgtagtaatt ggttgtattt
      361 agcaatacgg tcagtacgtg atagagaacc agttttgatt tggccagcgt ttgttgcaac
      421 agcgatatca gagattgttg aatcttctgt ttcaccagaa cggtgagata caactgctgt
      481 gtagccagct tctttagcca tttcgatagc ttcgaaagtt tctgttaatg taccgatttg
      541 gttcactttg ataaggattg agttagcaat tcctttttcg ataccttcag ataatttttg
      601 agtgtttgtt acgaacaagt cgtcaccaac taattgaact ttgtcgccta atacgtcagt
      661 taatttcttg aatccgtccc agtcgttttc gtctaatcca tcttcgattg agatgattgg
      721 atatttagaa actaattctt cgtagaattt gatcatttcg tcagttgttt tttcgccttc
      781 gcctgagtca gctagtacgt aaacaccttt ttctttgtcg tagaattctg aagaagcagc
      841 atccatagca agaacaacgt ctttaccagg tacatagcca gctttttcga ttgcttcgat
      901 gattacttca aaaccttctt cgtttgaacc aaggtttgga gcaaatccac cttcgtcacc
      961 aacagaagtt gctaatccgc gagctttcaa gattgaagct aatgcgtgga atacttcagc
     1021 acccatacgt agagcttctt tgaatgttgg agcgccaaca ggcatgatca tgaattcttg
     1081 gaagtcgatt gagttatcag catgtgatcc gccgttgatg atgttcatca ttggtgttgg
     1141 caatactttt gtgttgaatc cgcctaggta gtggtataaa ggtacttcta ggtagtcagc
     1201 agctgcacgt gcaacagcga tagaaacacc taaaatagcg ttcgcaccta atttaccttt
     1261 gttaggagtt ccgtctaaag cgatcatagc tttgtcgata gccatttggt cacgaacatc
     1321 gtagccaatg attgcttcag caatgatgtt gtttacgttg tcgacagctt tagttacacc
     1381 tttaccgcca taacgagctt tgtctccgtc acgtaattca actgcttcgt attcaccagt
     1441 tgaagcacct gaaggaacca ttccgcggcc aaaagctccg ctttctgtgt atacttctac
     1501 ttcgattgtt gggttaccac gtgagtctaa gatctcgcga gcgtaaacat cagtaataat
     1561 tgacatgttt tgtctctcct ttgagtttgt tttttcctaa gggatttccc tcatatacga
     1621 ttttagttaa ttttgctcat tagtgcaaac atttcttgca tttttcgcaa attttcaaca
     1681 attattttac agcgtctaac aatgataaga atgaatctgc ttgcaagctt gcgccaccga
     1741 ctaaagcacc gtcaacattt tcttttgcca tgtattcagc aatattttct ggttttacag
     1801 aaccgccgta ttggatacga actgcttctg atacttcttt tccataaagt ttttcaactg
     1861 tttgacgtac aactccacaa atttcgtcag cgatttgtgc gtcagctgat ttaccagtac
     1921 cgattgccca gattggttca taagcaataa ccatgtttga cacctgttca gctgaaagat
     1981 cagctaaacc agctgtaatt tgtccttcga tccattcagc agttttgcct gcttcatagg
     2041 tttctaaaga ttcgccgcag cataggattg gtgtcatacc atttgcaaag atagctttcg
     2101 cttttttatt gatgtcttcg tctgtttcat ggaaatattc acgacgttct gaatgtccga
     2161 tgatcacata atgaacacct agatcagcaa gagctgctgg tgaagtttca ccagtgaacg
     2221 cacctgagtt ttcccagtag cagttttgtg ctgagatttt tagttctgta ccttcagctg
     2281 atgtcattaa ttcttgtaag aataatgcag gagatccgat gactgaatca actttgtcat
     2341 ttgaagggat tttgtttttt actgcttcag caaattcttt tgcttctgat gcagttttgt
     2401 tcattttcca gttacctgcg ataataggtt tacgcattga ggaacacact ccttaattta
     2461 ttgaaaaatg gctagggagt catgaaaact cccttcacct tttgttcgat tactcgtcag
     2521 aattgagcaa acttggtcgt ttatttatcg ttgattgctg ctagtcctgg taattcttta
     2581 ccttcaagca attccaagct tgcgccgcca cctgttgaga tgtgtgtgaa tttgtcagca
     2641 aatcctaatt gttctgctgc tgcggctgag tctccgccac cgatgattgt tgttgcgcct
     2701 tctaagttag cgattgcttc acaaacaccg attgtacctt tagcaaagtt actcatttca
     2761 aatacaccca taggtccgtt ccaaacaact gtttttgcac cttgtaatgt atcagcaaat
     2821 aattttactg atgctgggcc gatgtctaaa gccatcaaac catcaggaat gtctccttcg
     2881 attacttgtg tttcaacatc atttgaaaat tctggtgcac agacattgtc gattggcaag
     2941 acaagtttgt cgcctgcttt ttcgattaat tcttttgcta gttccacttt atcagcttct
     3001 actagagagt taccgatctt gattcctttt gcttcgtaga atgtgtaagt cattccgcca
     3061 ccgatcaaga ttttatctgc tttaggaatc aagttttcga ttacgccgat tttatcagaa
     3121 acttttgcgc caccaaggat tgcaatcatt ggtcgttttg gttcttctac tgcttcaccg
     3181 atgaacttga tttctttttc catcaagaat ccagcaactg ttgggatacc agttgaagcg
     3241 atacctacgt tagaagcatg tgcacggtga gctgtaccga aagcatcgtt aacaaataca
     3301 tcgcctaatg aagcccagta tttacctagt tctgtatcat ttttgctttc ttttttaccg
     3361 tcaatatctt caaatcgtgt attttcgaat acgacaacgt cgccgtcatt catgttgcgg
     3421 attgcttctt ctaattgttc tccgcgagtt tcaggtacga atgttacttc tttacctaat
     3481 aattcgccta gacgttttgc aactggagct aaagattttc cttctttatc ttcttctgtt
     3541 tttacacgtc caaggtgaga gaaaaggatt gcttttccgc cttgttctag aacgtagttg
     3601 attgttggca atgctgcttt gatacgagtg tcgtcagtga tcacgccgtc tttcaaagga
     3661 acgttgaagt caacacggac aagaactttt ttgtctttta aatcgatatc tttcactgtt
     3721 ttcttagcca tttt
//
LOCUS       DACDIH010000134         3665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDIH010000134 DACDIH010000000
VERSION     DACDIH010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.135"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            65..766
                     /locus_tag="IV281_002719"
     CDS             65..766
                     /locus_tag="IV281_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP8781650.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            763..1884
                     /locus_tag="IV281_002720"
     CDS             763..1884
                     /locus_tag="IV281_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP8781651.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1899..3131
                     /gene="pepT"
                     /locus_tag="IV281_002721"
     CDS             1899..3131
                     /gene="pepT"
                     /locus_tag="IV281_002721"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP8781652.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3263..>3665
                     /locus_tag="IV281_002722"
     CDS             3263..>3665
                     /locus_tag="IV281_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylglycerol kinase family protein"
                     /protein_id="HAP8781653.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVI"
BASE COUNT         1257 a          649 c          815 g          944 t
ORIGIN      
        1 tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta gaaggagtga
       61 agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact ttgtccctga
      121 atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg cattgatatt
      181 aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt atgaatcagc
      241 acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc ttgcaaatgg
      301 tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg gtatgggagg
      361 ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa gcggacaaga
      421 acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc tgcaagaaaa
      481 tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat atgaaatcat
      541 tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga tgttcgggcc
      601 atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag agcttcaaca
      661 aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac gtcaaaaaca
      721 aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat cagcggacgc
      781 actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga aggagaccca
      841 gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat gacattagac
      901 gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct gattgctaaa
      961 catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca agaaaaaatg
     1021 tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa catggatatt
     1081 atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt ggagagttat
     1141 ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc tgtggatcat
     1201 gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg tgattatacc
     1261 ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg tgcacaacca
     1321 gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga ggttattttg
     1381 ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc ttatgaggaa
     1441 cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt aggacgagta
     1501 ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa agaagcgttc
     1561 caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt gaagcgcatt
     1621 gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca aagagcagat
     1681 gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag cgctgggttg
     1741 attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt tatcgagaaa
     1801 tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc tgaaacaaat
     1861 acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat cttttgccac
     1921 gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa acgactccat
     1981 cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa gagttaggct
     2041 tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta ccaagcaatg
     2101 ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca gattttaatg
     2161 cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg atcgtactag
     2221 atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa aattatgaag
     2281 gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac aaatcaggga
     2341 ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc cctcacggta
     2401 ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat aaattcgatg
     2461 ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta ggtgagttgc
     2521 agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa aatgtacatc
     2581 cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat tttcataatc
     2641 agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc ttccatttga
     2701 tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc gatcatgatc
     2761 gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag ttgaatcagc
     2821 gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac atgaaagaaa
     2881 tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc gagcttggta
     2941 tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc tcttacatgg
     3001 gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga ttcgaatttg
     3061 tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca gaattgaaca
     3121 caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca atgatggaat
     3181 gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc ctcttttttg
     3241 aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca aaagttcagg
     3301 gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag aaaagcagcc
     3361 agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg aattagtcaa
     3421 gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg gcacgattca
     3481 tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat tacttccagg
     3541 tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag aagaagctgc
     3601 tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca atgatgaagt
     3661 catta
//
LOCUS       DACDIH010000135         3638 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.136, whole genome shotgun sequence.
ACCESSION   DACDIH010000135 DACDIH010000000
VERSION     DACDIH010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.136"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..330
                     /locus_tag="IV281_002723"
     CDS             <1..330
                     /locus_tag="IV281_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8781654.1"
                     /translation="DFKPLGLAIKEARLSKGLTREQVGSMIQIAPRYLTNIENKGQQP
                     SFHVVYDLVTLLNISLDGFILGEENSHKSSKRLQVEAQLDCLDEDELVTIGKVIQAIY
                     GEKCKFN"
     gene            complement(844..1011)
                     /locus_tag="IV281_002724"
     CDS             complement(844..1011)
                     /locus_tag="IV281_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8781655.1"
                     /translation="MCPICGNKTRLKMREDTELKNFPLFCPKCRQEILIEITKFRITV
                     ITEPDAKTQSR"
     gene            complement(1099..3039)
                     /gene="tet(S)"
                     /locus_tag="IV281_002725"
     CDS             complement(1099..3039)
                     /gene="tet(S)"
                     /locus_tag="IV281_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(S)"
                     /protein_id="HAP8781656.1"
                     /translation="MEEIKLKIINIGILAHVDAGKTTLTESLLYSSGAIKELGSVDSG
                     TTKTDTMFLERQRGITIQTAITSFQRENVKVNIVDTPGHMDFLADVYRSLSVLDGAIL
                     LISAKDGVQSQTRILFHALRKMNIPIIFFINKIDQNGINLPDVYQDIKDKLSDDIIIK
                     QTVNLNLKPYVIDYTEPEQWETVIVGNDYLLEKYTIGKTLNIAELEKEENERIQSCSL
                     YPVYHGSAKNNIGIKQLIEVITSKLFSPTQLNSDKLCGNVFKVEYSDDGQRLVYVRLY
                     SGTLHLRDSVNISEKEKIKVTEMYTSINGELRQIDKAEPGEIIILKNELLKLNNVLGD
                     KKRLPHREILENPLPMLQTTIEPCKSVQREKLLDALFEISDSDPLLQYYVDTVTHEIV
                     LSFLGEVQMEVTCTLIQEKYHIEIETRKPTVIYMERPLKKSEFTIDIEVPPNPFWASI
                     GLSVTPLPLGSGIQYESLVSLGYLNQSFQNAVMEGIRYGCEQGLYGWKLTDCKICFKY
                     GLYYSPVSTPADFRMLAPIVLEQAFRKSGTELLEPYLSFEIYVPQEYLSRAYNDASKY
                     CANILNTKLKGNEVILIGEIPARCIQEYRNSLTFFTNGRSVCLTELKGYQVTNIKSAF
                     QPRRPNNRIDKVRHMFNKINLH"
     gene            complement(3040..3126)
                     /locus_tag="IV281_002726"
                     /pseudo
     CDS             complement(3040..3126)
                     /locus_tag="IV281_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance protein"
BASE COUNT         1169 a          606 c          563 g         1300 t
ORIGIN      
        1 gatttcaaac cattgggtct tgcgattaaa gaagcccgtc ttagtaaagg attgactcgt
       61 gagcaggtag gatcaatgat ccagattgcg ccacgctatt tgacgaacat tgaaaataaa
      121 ggacaacagc caagttttca tgttgtatat gatttagtga ctttgctaaa tatttctttg
      181 gatggattta ttttagggga agaaaacagt cataaaagct ccaaacgtct tcaagtagaa
      241 gcacagttgg attgtctgga tgaagatgaa ttagtgacga ttgggaaagt gattcaagca
      301 atttacggag aaaaatgtaa atttaactag ttcaataata cacaatggat taattaatac
      361 tatcaggaaa gattgattac aaagatgtaa tgaatttttt ctgatttttg ttaatcataa
      421 atagaaaatg aatttatctt tgttacactt aggaactaga gcgaatgata aatagttctt
      481 tggttactct tatcgctgca aagtacagtt cattttttgt tgttgggaaa attttctgga
      541 tttattgcat atcacttcat ccgactaaaa atttttgatt cccatagcga agttcgtttt
      601 tgttccttag gaaaaacttg actgatatac tagttttgaa atgacgagta actctgtact
      661 tcatcaataa tcaagtattg aattttaatt gtatactttt tatgcttgag ggatgtctac
      721 tgtaatacag ctactgcaaa aaaaatcagt acacttattt tcttataaag gatatcaaga
      781 actaagctca atggtatgca tagaaaagaa aaagccgata aaatgagatt ttgatatctc
      841 gaattatcgg ctctgcgtct ttgcgtctgg ctctgtaatt acagttattc taaacttcgt
      901 tatttcaatt aaaatttctt gtctgcattt aggacaaaat agagggaaat tttttaattc
      961 agtatcttcc ctcattttta aacgggtctt attgccacat atagggcaca gtatccacct
     1021 agtatttaca atatctatat cctcctgttc taattaacaa ctcaatattc attttaaaaa
     1081 tttctcttta gttcactatc aatgtaagtt gattttatta aacatatgcc ttactttgtc
     1141 tattctatta tttggacgac gtggttggaa agcagactta atgttagtaa cctgataacc
     1201 ttttaactct gttaaacaga cactgcgtcc atttgtaaag aaagttaaac tgtttcgata
     1261 ctcttgaata caacgggctg gaatttcacc aatgagaatg acctcgttac cttttaactt
     1321 agtatttaaa atatttgcac aatatttgga agcatcatta tatgctctcg aaagatattc
     1381 ttgtggtaca taaatttcga agctaagata tggctctaat aactctgtac cactctttct
     1441 aaaagcctgc tctagtacaa taggcgcaag cattcggaaa tctgctggcg tactgacagg
     1501 gctgtaatat agaccatact taaaacagat cttacagtct gttaatttcc aaccgtacaa
     1561 tccttgttca cacccatagc gtataccttc cataactgca ttttgaaatg attgatttaa
     1621 ataacctaga gaaaccaggc tctcatactg aatgccacta cccaaaggaa gtggtgttac
     1681 agataaacca atagaagccc agaaaggatt tggaggtact tcgatatcaa tggtaaattc
     1741 agattttttt aatggtcttt ccatataaat gacagttggt tttcttgttt ctatctcaat
     1801 atgatatttt tcttgaatca gagtacaagt tacctccatt tggacctcac ctaaaaaaga
     1861 tagcacaatt tcgtgagtta ctgtatctac ataatattgt agaaggggat cactatcgga
     1921 tatttcaaaa agtgcatcta gtaacttttc tctttgtact gatttacatg gttcaattgt
     1981 tgtttgtaac ataggaagag gattctcaag aatttctcta tgtggtaatc tttttttatc
     2041 tccaagtacg ttatttagtt ttaaaagctc attttttaaa ataataatct caccaggctc
     2101 tgccttatct atctggcgta attctccatt tattgaagta tacatttctg taacttttat
     2161 tttttccttt tctgatatat tgactgagtc tcgcaaatgt agcgttccac tataaagacg
     2221 tacatagaca agccgttgac catcatctga atattctact ttaaaaacat ttccacaaag
     2281 tttatctgaa ttgagttgtg tgggtgaaaa taatttgcta gtaattacct ctataagttg
     2341 tttaattcca atattattct ttgcacttcc gtgataaaca ggatataagg agcaactttg
     2401 aattctttcg ttttcctcct tttcaagttc tgcaatattc aatgttttcc caatggtata
     2461 tttttctaat aaataatcat ttcccacaat tactgtctcc cattgttctg gttcagtata
     2521 atctattaca taaggtttca aatttagatt cacagtctgc ttaattatga tgtcgtcaga
     2581 aagtttgtcc ttaatatctt gataaacatc tggcaaattt attccatttt gatcaatttt
     2641 gttaataaaa aatattatag gtatgttcat ctttctaagt gcatggaata gtatacgagt
     2701 ttgtgactgt actccatctt ttgcagagat tagcaaaata gctccatcca aaacagataa
     2761 tgaacggtat acatctgcca aaaaatccat gtgtccagga gtatctacaa tatttacttt
     2821 aacattttcc cgttgaaaag atgttattgc ggtctgaata gtaatacctc tctggcgttc
     2881 caaaaacata gtatccgttt tcgttgtacc gctatctaca cttcctaact ctttaattgc
     2941 tccgctactg tatagtaagc tttctgtcaa agtagttttt cctgcatcaa catgtgctaa
     3001 gataccgata ttaataattt tcaattttat ttcctccatt caaaagctca aaaagcagaa
     3061 aaaatcccag tgataaatac tcttatcact gggattttaa tacaaaaata gctgtatact
     3121 acacactgat agccacagat gctttatgat aaaatgataa aagtattctt aaactgggca
     3181 caaaaaacca gtccttattt taaataagga tgcttaatta attattcccg ctatctaatt
     3241 gacagtttat ttaagaatac cttgccgcat ttttattatc tccttttgaa tgttatctta
     3301 attctatcat atgatagaat gcttgtaaag ccaaccatat tttttgaacg aaagggaaat
     3361 taatgagaaa ttaatggaag ttagtgtagt agttgaagat aactcttcag aatggctatc
     3421 aaaattcagc gatttaataa cgatttacaa gttcataagg gattctttat tcatccagct
     3481 attatattac ttttcaggtc aataggacaa ctgtccacaa cctttctaca aatatattac
     3541 gtataatttt tgaaatatat actgcttaac aaaatatttt gcagtggttc caatagtttt
     3601 gaattgttaa tattaagttt gacttggaaa atatcatt
//
LOCUS       DACDIH010000136         3402 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.137, whole genome shotgun sequence.
ACCESSION   DACDIH010000136 DACDIH010000000
VERSION     DACDIH010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.137"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..278
                     /locus_tag="IV281_002727"
     CDS             <1..278
                     /locus_tag="IV281_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAP8781657.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            421..642
                     /locus_tag="IV281_002728"
     CDS             421..642
                     /locus_tag="IV281_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8781658.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            658..1500
                     /locus_tag="IV281_002729"
     CDS             658..1500
                     /locus_tag="IV281_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781659.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1563..3365
                     /locus_tag="IV281_002730"
     CDS             1563..3365
                     /locus_tag="IV281_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAP8781660.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
BASE COUNT         1232 a          469 c          703 g          998 t
ORIGIN      
        1 aagaactcat caagctggct aataagggtc aagattatga acatctagca gatgagattg
       61 atgagctgag agacaagcga cagctccttt tagtagaaga tgcctccctc agtggcgaga
      121 acgagcgaat caatgagctg attgaattta tccgcaagaa caaattccgt accttagagt
      181 acgatgataa gcttgtaagg aagataatcc agagcgttac agtctatgaa gaacacttcg
      241 tcatatcctt caaatctggc atcgaaatgg aaatatgaaa accagaagta aatagcccat
      301 gattctacag tagagtcgtg ggttttcttt tgcttgctga ttataataat attttaataa
      361 ctattgttat tataaaccaa atgatttata atattcttag cgtaaacttg gaggtgctgt
      421 atgactacgg cagaaatgat taaagaactg tgtgagcaaa tgaatataag tgtttccgaa
      481 cttgctagac gtattggaca gactccacag aatttcaata agaaattaca acgagaaacg
      541 gtaaccttag atgagttgaa agcgattgct gatgtgctag gtgtcaagtt tgtgcaggct
      601 tttatattgc ctgatggcaa tgaaataaag ataggtaatt aggaggtgta atttgtattg
      661 aaaatcataa caataaatga tgttgagtat gctgtatttg ctgctaatga aggtacttcc
      721 aaaccacaac ctcatatcat agaaacgaaa tctggtacta ttccagaagg aaaacaactg
      781 agtttattaa aagaatatct taagcagaat gatatttccc caataaaagg agcaacaaca
      841 tattggtgca ttgacaaggt tcttaagctt gattcaagta aagaaaaaac tataagtgaa
      901 acaatccata aacaaaaata cttatcttta actgaagaaa atattgagaa acagcataag
      961 tttgtaggtg caagctctaa ttatgggaaa gaaggtctaa taatacatga tgttttgaat
     1021 gcatttcctc ttcataatga tttaaatact attgcgatga agattgcggt aattgatgta
     1081 acaaattcaa cacatctttc tcaatataag agcaggttgt ctctatatga tttggctaaa
     1141 gtaattctgg agattccaaa ttttgatgat cggttggcaa aaggagatcc tcagttgata
     1201 aatattatcg caaggaacat tggtgcagtt aatatgttct catttgcatc caagtactgt
     1261 acatatcata atgtggaagt gtgtgggaga gatgattact ccatctttga tggtatcgtt
     1321 aaaaatactc ttccacacta tatccaagga ctaactacga ataagattga tacgtggcga
     1381 agaagttttg attacgaggc atttaacgaa tgtgttggaa aactacttga tgaaaataat
     1441 atacatattc cttttagaag aagaaagtta gatcacttct tgtggtatgc gaataggtag
     1501 aatataagaa aaaggcatcg taaaagtgat tctggataaa attaatgggg gaggaaataa
     1561 tagtgggatt atttgatttt ctaaagaaaa aacaaggaaa ggttagtaaa cacgttgatt
     1621 atcaactggc ttctagtcta tttcgtactg gtaaagtttt tgctgaaaaa ttggaagtgg
     1681 acatatcaaa tgtagatgag tttcgtaaaa cttttatcgc ttttgatgtg gaaacaactg
     1741 gcctaaattc ttcttcagat agaattgtag agattggtgc ggtgctattt aaagaaggag
     1801 ttccaattca atcattcgga actcttgtca atccaaaagt tagaattgca gcctcagctt
     1861 cggcaataaa taatataact aatgaaatgc taaactctgc tccaaatgag caggaagttt
     1921 acccccagtt ggtagaattt ttaggagatg cactagatgg aaaaatcact atgtgtgcac
     1981 acaatgcaag tttcgatttt ggatttttaa gtaacacatt ttctagactt gggtatgatg
     2041 ctaaaattaa gtatgttgat actctgagtc tctctagaaa acatataaaa ggcattgtaa
     2101 atcataagca agagactgta gcggaatatt tcggattaat aaatgacact gcccatagag
     2161 cggaatcaga tgcagatata tgcggaaaga ttttttgggg tgttttagat attattgagg
     2221 agtcgttgga agaacaaaaa aagcaaatag aaaagatgac tccagacata aaagaacttg
     2281 aagcttgtgc ttttattcaa gatattattg agaaaaaagg atgcgacacc agttggatca
     2341 ggtatcgaaa aaacagtaat aattatgtcg atgtaacttg tctatataca ttcttaaagt
     2401 ttaagtttgc taagaaggga aattacatca ttattagaaa agatgctaca aatggaattg
     2461 atttacctat agaaccttgt actgtaagtg aaggcggtac aacaaatgtg cgagtctatt
     2521 ttcgtagccc ctttgactta acgcctcttt caaattatat tttcagtgct tatagcgatt
     2581 gttataattc tatgaatgaa tatatgagtg ttagtaatta cactaaaaaa gaagcagagc
     2641 aaagccttag tatgctgaaa tctatttcaa aatcagcgat gaaagagtta ttattcgatg
     2701 caaaaaatcg tggatatgat gagactgaag ttaacctaaa tattgaaaaa gtgatatcta
     2761 gagaagatgt aattattaac gggttgcata atcgagtttc attaaacaag atacgaaata
     2821 tagggaattg ggagaaagga tttgatgcag gttatccata ttgggaacgt ggagaaactg
     2881 ctaggaaaaa tggcaaagta gatgaagcta ttgcactttt tgataaagct cgatataacg
     2941 gctatgatgc tcctgcgcta tatgattctt atgctaaagc ttatcgaaaa attaaggatt
     3001 acgataatga aattctcatt cttgatgaag gaattatgcg taaaacacgg cacgatgttg
     3061 gtactttaga ggctcgtagg gataaggcaa ttaagctatt atttgctaaa caagaggcag
     3121 agcggatagc taaggaaaaa tctgattttc taaaagcgaa caaaaaagag gatgcgatta
     3181 gtgagcctag caagaagcgt ggaagacaga ttattcaaat gactgatgat ggaacaataa
     3241 taaaagaatt cgatactata gcttctgctg taaaagaagt tggcgttaac tcaaaaagta
     3301 ttagggatgc tgcaaatgga gttcaaaaac atgctggagg atactactgg aaatacaaag
     3361 attagcagat aatctaagat aacctaggat cttcttaata ga
//
LOCUS       DACDIH010000137         3353 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.138, whole genome shotgun sequence.
ACCESSION   DACDIH010000137 DACDIH010000000
VERSION     DACDIH010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.138"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(552..2165)
                     /locus_tag="IV281_002731"
     CDS             complement(552..2165)
                     /locus_tag="IV281_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP8781661.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2306..3214)
                     /locus_tag="IV281_002732"
     CDS             complement(2306..3214)
                     /locus_tag="IV281_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8781662.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1093 a          568 c          582 g         1110 t
ORIGIN      
        1 ttaaataaat aggaacaaaa aaattaaatt atttaataca cacattaggg atcattcatt
       61 gaaggattag aagacaacga ggatggcgaa aaatatgatt acaatagaaa agtcgttcta
      121 aaccaatcag ttcctagtat cacacaacgc aagccagtcg atttggtgag attagaggag
      181 tgaaagcaaa ctttattata taagaaaaaa tattcgaaag ggatgctagt ttaggtacta
      241 gtgctgatag attaatatga tgcctcgtaa tattaggcgt agttactcta gagacatcac
      301 tatggactat gatttgtctc agagtcacaa catgtttttt tatgaaaaga aacagctaaa
      361 ataattaaat tgcgcaatgt ttaaactttc ttaaactata acctaataaa tgtgaagaaa
      421 taatgcacaa tttcttcaca tttatcttct attctatata cataccaacc aacaacccta
      481 tatacttttt catttttact cctcaagtaa aaataactcc tagaccgcgg ctacccaccg
      541 cggtcgtttt tttattcttc aatcaaaatc gctttgatac ccaaacctgc caagattcgg
      601 gcagaagtat aagtattcaa tcctcttctg aaagtaatgt aaactttttt atcttttggg
      661 atttcattga ttcgatctcg taactcattc attggaatat tttttgttgc cgagatactg
      721 ccagaaggtg ctttattagg ttcacgtata tctaaaaaga acgcttcttt caaatcgttt
      781 tcgggtatat cacttgcttt gatcgtttct acgatattcg tcatttgatt gattgcgaca
      841 taaccagcta tattcaacgg gtcacgggta gtagaatacg gtggagagta aggcaactcc
      901 aaatcaggca aatcaaagac tgtcaagttt ccggtgattg ctacagaaag ttctcccatc
      961 cgtttatcga cacctttttc tccaaatgct tgtccaccta aaatacgacc gctatctgca
     1021 tcaaaaatca gctttagatt taatcttgta gcttctggat agaaataggc atggtcaaag
     1081 ggagtgataa aaacagtttt ataatttgtt atgcctgatg cttttaattc atgttctgtc
     1141 attcccgcat aacttgctgt atgatcaaag atttttgcga cacctgcacc aatatatccg
     1201 cgatagcgca taggcgtacc atttaaaata tctgccagca tatgtccttg acgattggcc
     1261 gcactggaaa gcatactttg gatcggctga ccagtcacta cactagtcgt ttcgataata
     1321 tcgccaatcg catagatgtc cggcagattg gtttgtaact ggtcatcaac aatgatctga
     1381 cctgtatctg ataattgtat gccggttgct ttcaccactt cattcttcgg agaaacacca
     1441 acagcaaaaa ttagcatatc agcagaaagg acggagccat ctgataatac tatttctcga
     1501 cctttatcac ggatctcagt aactcttgtt tctaaatgaa cggctaatcc ttctttcagc
     1561 aatttgtcat aaactaaatt agcaatttct ggatcataag ggaaagcgac ttgtggcaat
     1621 tgatcgatta aagtcacgtc taagccgcga tgcttgaaat tctcagctaa ttccagaccc
     1681 ataacacctg caccaagaat gacgacatgt tgtggatttt tttcttcaag gaacgatttg
     1741 attcgatccg catccgtcac actacggagg acaaagccgt tttctgcttc aaaaactccg
     1801 ggaatatccg gataatctgg tctagcacca gaagagatga tcaacttatc gtaatctgtt
     1861 tttatttctt catttgtgga taggtcttta actgttaatc gcttagtaga aggatcgatt
     1921 gctgttactt cgtgtttagt aaagacatcg atgttgtttt ttgttttcaa tatttctggt
     1981 gtacgttcga tcagcgagtc acggtcagtg atcacacctc ctaaataata aggcaaagca
     2041 caacttgcat aagaaatatt ttctccgcgt tcatagataa tgatttcgtg cgcttcattc
     2101 aatcgacgga aacgagtggc aaatgaagga ccgccagcta caccgccaat gacaacaact
     2161 ttcataaaaa catctccttt tctacgttga tcgtactatg ttatggtaaa aaacagcaaa
     2221 taattgtgcc ggaaaattat tgtttatttt gaatagacag aaaatataga gaaataattt
     2281 gtggataaaa cctaaaaatt tttttctatt ttaaggatag ataatacttt gaaggggata
     2341 accctgtata ttttttaaaa gctgaagaaa aatgatggat cgaatgaaat cctaatatat
     2401 ctgcaatttc tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt
     2461 ttagttcttt aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta
     2521 agctggctgg actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa
     2581 gatgttcttc taaatatcgt atgattggat caactaattc atctgaataa agaatatgct
     2641 tttttttagg aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat
     2701 aatgccttgg taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa
     2761 gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg
     2821 aaatagctgg cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag
     2881 taatgataga tgttatttct tgatctatag aaaaagattg tcccgtaata tgtgctaata
     2941 attgctcatc aaaataaaac tggataatga taaaagtttg tgtttccttt tgagtagaaa
     3001 agatttggac ttcttgctgt ttatcaatga ataaaaggga aggattatct agttctatag
     3061 atttatgtgc aaattcagca tataaattag cgctttcatt tattagtagt cgaaaatagg
     3121 gagcagcaga taaatgaaga tgggtatcta tggaataggt tagtgttttt ttgacaagat
     3181 tggaggattg gttgtttgtt aacaaaagaa ccactttcct ttctcttttt atataaaaag
     3241 ctttctattt ataattaatg actattataa caaaaatgaa aagccaaaac gacaagtaaa
     3301 atattttttt atataaatat ttgtgttact aattccgtta atatacataa gtg
//
LOCUS       DACDIH010000138         3324 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.139, whole genome shotgun sequence.
ACCESSION   DACDIH010000138 DACDIH010000000
VERSION     DACDIH010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.139"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1155)
                     /locus_tag="IV281_002733"
     CDS             complement(<1..1155)
                     /locus_tag="IV281_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8781663.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
     gene            complement(1158..>3324)
                     /locus_tag="IV281_002734"
     CDS             complement(1158..>3324)
                     /locus_tag="IV281_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8781664.1"
                     /translation="FKISEDGKLLHLYYGENLPDNDYSHLIEMHHRPMTTYRNENDLL
                     YSLEHLKQEFPEYGTTDFRHPAISLLQKNGSRLSDFVYQGYEVEEGKPKLEGLPATYV
                     DNNDESKTLKVYILDSVTNVQVELLYTIYRDYPVITRSARVMNKGKDIQIIENISSLS
                     LDLPDANYEWQQLSGAWGRERTIKSRTLQQGIQSIESTRGISSHQHNPFVTLKRKNTD
                     ENQGEAIGAALVYSGNFLIQAEVDTWDVTRLQVGINPFGFNWKLNPGEIFTAPEAILV
                     YSSKGLNAMSQTFHQLFRKRLARGKWREKDRPVLINNWEATYFDFDEEKLVNIAKKAF
                     DVGIELFVLDDGWFGERENDCAGLGDWHVNPKRLPRGIGSLSEKIRSFGMKFGLWFEP
                     EMVNKDSELYRTHPNWIISTPNRKPNHGRNQYVLNFGLDEVVENIFDQMCKIIDESNL
                     DYIKWDMNRPLTDVFDSHLSADQQGEVFHRYVLGVYRLYEKLITKYPNILFESCSSGG
                     GRFDPGMLHYAPQAWASDDSDAIERLKIQYGTSMLYPLSSIGAHVSIVPNHQTNRITP
                     IKTRGNVAFFGAFGYELDLNELSEGDLRIVKEQIAFYKKYRTIIHNGTFYRLLSPFDD
                     DNQTAWMVVSHDKKTAIVAHYKTLNEVNAPYRRLKLKGLDNDGLYNVGQNSLRSGMEL
                     MRAGLVCSDASSGQINDQNRRPETFDFDSKIWVITQEEK"
BASE COUNT         1079 a          625 c          482 g         1138 t
ORIGIN      
        1 tctagtattt tttttatttg tcgcaaaaaa tgttacaaat aggatcacaa taaatattcc
       61 tgcgaataca aaaaatggag cagtcaaatt tgtacttctt gttagaaatt gtgctaattg
      121 catagcaata ggagctataa aattaccaat attacagcca ataaaaatca atgaagtagc
      181 aaaattaatt gtattctttg tagtgacttt atctaagtta ctaaagataa atgggaagca
      241 ccatgatcct gttatacttg ataaaaataa acccgttagc aggaacgtca tattaccgtt
      301 tgacacagca attaacaaat ttgaaattat aaagaatata attcccaagt atagggttcc
      361 ttttcctatc cacttattta ttggtccaaa cataaatcct gctagtattc ctaagatagg
      421 cattagagct aaatagttac tagcattaaa gtttacccct ttaatttcag ttgcaattga
      481 tgcaaatcgt acagaaatag caatactatt cattacgagt aagattgcaa atagaactag
      541 ggcaaaaacc attggattca tcttttcttt tacatgattt tcaggtatgt cactattttc
      601 atctctaatt tcaggaactt ttagcgcgaa taagagagct acaggaaacg caattgcata
      661 gactaaaaac gaagccgtcc aattaatgtt cactaaaact ccagcaagaa aaataaggat
      721 tgtctgacct aaagcttcca ttgaatttct aatacctaat agcgttgctc tagtattgcc
      781 agagtataaa gagctgatat atgtcacagc caatgaatta tatactccta aaccggcacc
      841 taatattact cgactaataa gaacggtttg gaaggtagct gtaaatactg gaataattcc
      901 tcctatacca gctaataata aacctaaaat tattgtcttt ttcattccta ttcttttggc
      961 aaagtaacta gataataaga taaacaaaat aacagttata cttggtgcag ttcctaaaag
     1021 ttcactttga ctttgtgata tccccaaaga ctgtttcatt tgtggaataa caccattaat
     1081 tgcttgagag cttgttaaca ttagtgaaat acttaagatt gctccttttt gaagtaagct
     1141 tttttggtta ttcatctcta cttttcctcc tgtgttataa cccaaatctt ggaatcaaaa
     1201 tcaaatgttt ctgggcgtcg attctgatca tttatttgtc cactagatgc atctgaacag
     1261 actagtcctg ctctcatcaa ttccattcca gatcgcaaac tattttgtcc tacattataa
     1321 agtccgtcat tatctaaccc ttttaatttc agtcggcggt aaggagcatt tacctcattt
     1381 aatgttttat aatgagcaac aatagccgtt ttcttatcat gactaacaac catccacgcg
     1441 gtttgattat catcatcgaa tggtgataat agtctataaa aagtaccatt atgaattatg
     1501 gttctatatt ttttataaaa ggcaatttgt tcttttacaa tcctcaaatc gccttcactt
     1561 aattcattta agtcaagctc atatccaaat gcaccaaaaa aagctacatt tcctctagtt
     1621 tttataggtg ttatacggtt tgtttgatga tttggaacaa ttgatacatg agcaccaata
     1681 gacgaaagag gatacagcat actagttcca tattgaattt ttaacctttc aattgcatct
     1741 gaatcatcac tagcccaagc ttgaggtgca tagtgaagca ttccaggatc aaagcgccca
     1801 ccacctgatg aacaagactc aaatagtatg ttaggatatt ttgttataag tttttcatat
     1861 aagcgatata cgcctagtac atatctgtgg aatacttctc cttgttgatc tgctgacaag
     1921 tgactgtcaa atacatcagt caatggacga ttcatatccc acttaatgta atctaagttt
     1981 gattcatcaa tgattttaca catttgatcg aaaatatttt caacaacctc atctaaccca
     2041 aaatttaata catattggtt cctaccatga ttaggttttc gattaggtgt agaaataatc
     2101 caattcggat gtgtgcgata taactcacta tccttattca ccatttcagg ttcaaaccat
     2161 aaaccaaatt tcataccaaa actacgaatt ttttctgaaa ggctgccgat tcctctaggt
     2221 agtcttttag gatttacatg ccaatctcct aatcctgcac aatcgttttc tctttctcca
     2281 aaccagccat catctaatac aaataattca ataccaacat caaatgcttt tttagcaata
     2341 tttaccaact tttcttcgtc aaaatcaaaa tatgttgctt cccaattatt aattaagaca
     2401 ggacggtctt tttctcgcca cttaccacga gctaagcgtt ttctaaatag ttgatgaaaa
     2461 gtttgactca tggcatttaa acccttactt gaataaacca gaatcgcttc tggtgctgta
     2521 aatatttctc ctggattgag tttccaatta aatccaaatg gattgatgcc aacttgtagt
     2581 ctagtaacat cccatgtatc tacttctgct tgaattaaga agtttcctga gtaaactagt
     2641 gctgcaccaa tagcttctcc ttgattttca tcagtatttt ttctttttaa agtaacaaat
     2701 gggttatgct gatggcttga aatacctcga gtagattcga ttgattgaat tccttgttgt
     2761 aaagtacgcg acttaatagt tctttctcta ccccaagcac cagataattg ttgccattca
     2821 taatttgcat cgggtaaatc caatgataaa ctgctaatat tctcgattat ttgaatatct
     2881 tttcctttat tcataactcg cgcgctacga gtaataacag gataatctct ataaattgta
     2941 tataataatt ctacttgaac atttgtaact gaatctaaaa tataaacttt caatgttttg
     3001 ctttcatcat tattatcaac ataggtagca ggtaaacctt ctagtttagg ttttccttct
     3061 tccacttcgt acccttgata gacaaaatct gataagcgag agccattttt ctgtagtaat
     3121 gaaatagcag gatgtctaaa atctgttgta ccatattcag gaaattcttg ttttaagtgc
     3181 tctaacgaat atagcaaatc gttttcattt ctataggttg tcataggccg atgatgcatc
     3241 tcaattagat gactatagtc attatctggt aagttctcac cataatataa atgcaataat
     3301 ttaccatctt ccgatatctt aaaa
//
LOCUS       DACDIH010000139         3295 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.140, whole genome shotgun sequence.
ACCESSION   DACDIH010000139 DACDIH010000000
VERSION     DACDIH010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.140"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            227..1873
                     /locus_tag="IV281_002735"
     CDS             227..1873
                     /locus_tag="IV281_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8781665.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            1864..1992
                     /locus_tag="IV281_002736"
     CDS             1864..1992
                     /locus_tag="IV281_002736"
                     /inference="COORDINATES: protein motif:HMM:NF015727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781666.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDHRKKRLP"
     gene            1959..3023
                     /gene="uxuA"
                     /locus_tag="IV281_002737"
     CDS             1959..3023
                     /gene="uxuA"
                     /locus_tag="IV281_002737"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8781667.1"
                     /translation="MDGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPRERIKEL
                     KEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVICYNFMP
                     VFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEPERLAHI
                     KSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLPRIVKNR
                     EDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRNIKFLAN
                     RDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGLYDRALG
                     AVYLNGLWEAIEKGNKSKDN"
     gene            complement(3131..>3295)
                     /locus_tag="IV281_002738"
     CDS             complement(3131..>3295)
                     /locus_tag="IV281_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8781668.1"
                     /translation="LKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMD
                     QSKKENQVTS"
BASE COUNT         1086 a          558 c          675 g          976 t
ORIGIN      
        1 tacgaatcgc ttgataagct tgtgattgca agctgctttt catctttatt cctccaaaat
       61 catttctatg aacgtagtat aacagaagag gtctgaaaaa caatctggta tgtcacatta
      121 actagtaaat ttgtataaat agtaaaattt tggttgacat agcgattttt attgtataaa
      181 attcacttga atacaaaaac taatatgtta gtttaggagg ggaaaaatga ttgatttaaa
      241 caactggagg aactttatcc ctgaattatc tcaaaaagaa gtagaaatac ctcattttga
      301 ccgtgcatca ttaagagaaa atggacaaaa aaatcctgtc tggatccatt tcggtggcgg
      361 aaatctttat cgaggatttc atgcagaaat cgctcaacgt ttagcggatc taggcgaatt
      421 gtccactggt gtcattgttt gtgaaacatt cgatgaagaa ataattgaaa gggcttacca
      481 agattttgat gatttgattc ttgaagtggt catgccaagc agtggagagt tcgtcaaacg
      541 tatattagct gcgacagctg acagtctgta ttgtcatcca gattgttctg aacactttga
      601 taagatgatt cgacactttg aaaatcctag tttacaatta gttacttttg ctattactga
      661 aaaaggttat cgattgaaag atacaaataa taaatatata ggaccgattg cagtcgatat
      721 aaaaaatggt ccaaagaagc catcacatac aatgagtatt cttgctgcgc tactttataa
      781 acgtttttta tctggagctt ggccgattgc acttgtctct acagataatt tttcacaaaa
      841 cggtagtcaa ttacgtactt ctttgctaac gatcgcaaaa gaatggcaaa aggaaggtta
      901 tgtacaaaat acatttatcc aatacttatc gaatgaagaa attgttgcgt ttccatggac
      961 gatgattgat cgtatcaccc cgaatccttc cttgtcagtt agcaaaatgt tggcaaaaga
     1021 gggcttttca aatatgtcga ttttccaaac cgcaaaagga aataatattg ctccatttgt
     1081 caatacagag gacatccatt atttaatcat ggaagatcat tttcccaatg gacgtccgaa
     1141 attcgaaaaa gctggggtat tcttgactga tcgtgcaacg gtagataaag cagatacgat
     1201 gaaggtgacg acttgcctta atcctttgca tacgtcttta gctattatgg gttgtttact
     1261 ggggtatagg agcatagctg aggagatgaa agatccagat ttatcgctgc tagtttctga
     1321 aatcgggtac aatgaaggcc tcccagttgt ggagtctcca ggtatcattg atccaaagat
     1381 ctttctggaa gaagtaatta ctgtgcgctt ttctaatcca aatattcctg atatgcctca
     1441 gcgaatcgcc acggatacct ctcaaaaggt ggcgattcgt tatggtgaga cgattaaaaa
     1501 atatatggaa atagaagtta aagacccgca gacacttgta ttcataccat tgactctcgc
     1561 tgcttggctt cgatacctta ttggcattga tgatcaagga aaaccattca tcccgagtcc
     1621 agacccacta ttgacagact ttcagcataa gctttccgat attcaactgg gtacgcaaga
     1681 tacatcacat attcatgaga aaatggctcc tattttgtca aataaagaaa tttttggtgt
     1741 agatctttac caagcagagc ttggcagaaa aatagaagaa atgttcatca agatggtagc
     1801 aggaccgaat gccgtacgaa aaacattaca ggattactta caagaacatg gaggaaattt
     1861 atcatggaaa tgacttttag atggtacgga tcacaggaag aaaagattac cttagatgaa
     1921 atcagacaga ttcctgcggt caaagggatt gtaggcacat tgatggatca caggaagaaa
     1981 agattacctt agatgaaatc agacagattc ctgcggtcaa agggattgta ggcacattga
     2041 tggatatacc agtaggagag gtatggccca gggagaggat caaagaacta aaagaagaaa
     2101 ttgagtctcg tgggttaaca ctaaaagtaa ttgaaagcgt caatgtgcat gatgccatta
     2161 aaattggatt accttcccgt gagcagtata tcgaaaacta taagcagaca atccgaaact
     2221 tggcagaata tggaatagaa gtcatctgct ataattttat gcctgtattt gactgggtga
     2281 aatctgacct tgactaccgc ttagaagacg gctcatccac gttagctttt atatctgatg
     2341 atattccaga tgatccaaga gaaatcgtcg cgcgcatcga acattccagc aatgaatttg
     2401 aattgccggg atgggaacct gagcgcttag ctcatatcaa atccttgctt gaagcctatg
     2461 catcagtaga cgaagaaaaa cttcgagaaa attttgccta ttttctccaa tcaatcatcc
     2521 caacatgtga agaggtaggg gtcaaaatgg ctgtacatcc agatgatccg ccatactcga
     2581 tttttggatt acctcgaatc gtaaagaatc gagaagattt agattggttg tgccgcatag
     2641 tagacagccc ttataacgga atcactcttt gtacagggag tattgcagaa gatccagaaa
     2701 atgatgtata tgggatttta gcagaatttt gtcaaagaga tcgaatccca tttgcacatg
     2761 ttcgaaatat taaattttta gctaatcgag atttctatga ggcaccgcat aaatccgaat
     2821 acggttcatt agacatgtac cgcatactaa aaacaatgta tgacaacgga tttaacgaat
     2881 acatccgtcc tgatcatgga agaatgatct ggaatgagac aggaagacca ggatacggct
     2941 tatatgatcg agcattaggt gctgtttatc taaatggatt atgggaagca atcgaaaaag
     3001 gaaacaaaag taaagataat tagtgaaaaa aactgtaatt ttattatgta aataagaagg
     3061 atgttcaaat tcttgataca taaatccgga ggttgttaat aagcattcgg atttatgtat
     3121 cttatttggt ctaggaagta acttgatttt cttttttcga ttgatccatg agtgcttctt
     3181 cttttaattc aaagatatat tctgagatcc tcataatcac ttttttccag tcttctggtg
     3241 tgatgattcc ttttaaaata tagatttttt tgatatgaga aggcaattgt ttcaa
//
LOCUS       DACDIH010000140         3149 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.141, whole genome shotgun sequence.
ACCESSION   DACDIH010000140 DACDIH010000000
VERSION     DACDIH010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.141"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..2322)
                     /gene="esp"
                     /locus_tag="IV281_002739"
     CDS             complement(<1..2322)
                     /gene="esp"
                     /locus_tag="IV281_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP8781669.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFT"
     gene            complement(2940..>3149)
                     /locus_tag="IV281_002740"
     CDS             complement(2940..>3149)
                     /locus_tag="IV281_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781670.1"
                     /translation="KQTLFNLIKLDAKISSYLFFLIHDEFMDYQKLDQLIERESWEDY
                     YVGLTFSEKLNNYKLIDYKYLSESN"
BASE COUNT          924 a          604 c          466 g         1155 t
ORIGIN      
        1 ggtgaaggtc actttgtggt agcctgcagg aatgttttct cctggatttt cataaatatc
       61 tttacttaca gttactgcta aatcggtcgt gcctacagaa ccatcttggt aaacaatttt
      121 aataggaatt gttccagtaa tcggctccga ttctcctaca cctttagctg gagtgaatgt
      181 aacaatacca gtgtttgcat caacacttgc attaccgaag tacttattat agacatctga
      241 aattgaaaag gcatctttag ctaaaggaac atctggttta tcaaatgtag gttttccact
      301 ttgaactgtc gtttctggag ttgttgttat aggtacgtat gttgcatcat tttccatagc
      361 ttgagaagct tttacagcaa actctacatc cacgtcgaaa gttcgatttc cgatcttaac
      421 agttacaata cctttcgtac tatcagtaac gttagaagta tcaggctctg ttttccatga
      481 atactctgca tcttcaggta agtttttatt attccgaatt gattctttag catctggttt
      541 aaatccaact accacggttt gtttatcaac cgcttttggt gattccttaa taacggttga
      601 accttcttct ggtttatcaa aacctggaga aacgatttgg atttttaatt catctttcgc
      661 gattaattta cttgaatcta cacccgtaaa ttcaaattct acagtatctg aaattggagc
      721 cccatctttt tcatttggag cgatagtttt ttctgctaat acagtacctt ctttatcaac
      781 aagttgggct ttgtgacctg ttcccgctaa ctcgtggatg aatactttcc ccttaactgt
      841 tgtgtcaaca tcatatactt cttctaattc aggtttcccg atatatggat tcgtgtctcc
      901 gctctcttct ttatcgtcag ggtttccatc accatcaata tcttgcaaat aataataacc
      961 tgttccataa gtgttctgga ttaatttttt attcgtatcc gacaaccaag aatcaaagat
     1021 gaaatcttca ccaaatggtt tattttcttc aacttgtgct gttgctttca tatcttctaa
     1081 gatttcatct aatggcttag tcaattgtaa aacatatcga ataacgactg gacgtggact
     1141 tgcattagca aaatcgataa gtttagttaa atcattatta gttattgaac ctatgccata
     1201 attttcatga ccttcccgtc gctcgattgc taaatcagct acttttgttc cggattcaaa
     1261 cgaaacatca tatgttactt tgtcagcctg aaccatgtgc atctgtatac ccgctacata
     1321 tggaactaat cgttcatcaa ttttgtaatt agcataccaa ggcgtgccct ttgcggaaga
     1381 tgaatatgag aaattataat ttttcgttaa cttatgatct aagattatag ctccattagc
     1441 acctgtggat cctgaaccat cttttgtttg agtattaggt ttctcatatt ttgcacttac
     1501 tgatccatgt ttaaactggt caggattatt ttcagataca tctaagctat tatagaaatc
     1561 atctgaatct gttaaaaagt agcccgtact atcaatacct ggtacaattt tatcatcgtc
     1621 ttgatgactt accaagtaga ttcgatacat taaacgatcg gtcttgagat tgtctatgcc
     1681 tatctcttta aaaatttcat ctaggctctt ttcaaacgta attaccccat taggagcggt
     1741 ttgcgtatcg gttgtttctg ccccagcaaa taatccatca cttgcacgaa tataattgac
     1801 cttccaaata tttgtaggtc tacccagcga atcattaatt cttacaaagg tccgtctaga
     1861 agatccaatt cctttctttg gatcgacctg aattttttct acatatttag ctaatcgttc
     1921 atctaattga agacggattt cccagttttt agttgatgct gcaatattat gttttccgta
     1981 aacttcaaat ccaattgtat tgccttttag tgtgtctcca gttagttctt gaggactaaa
     2041 ttgtaaatcc gaaaagttat aatttttcgt ataaccagga ttctcctgaa aagtattccc
     2101 cgaagctact ttgtcaccta cttgtacgga gcttatcgct gtttcaagtg ggctgcttgg
     2161 agaaatttca tcttgtgctt taactagttc agttgttccc aagacaagac caacaccaat
     2221 caaaacagat gcaaccccaa cacttaattt tttgatactg tacttcaata ctcgtttctt
     2281 tgttttttct agaaatactc tcttattatt cttgctaacc attattcaaa tcaactccct
     2341 taataattac tttcctagta agaataaaag ataaatctga tacgtattat tcacagaata
     2401 ggaatcttac taaccttatg ttaactaatt tacttagaga atttcatgaa cattacaaca
     2461 aaaaaaagta catttcttca tgtttttttc ggatatttgt gctttttaac cattcagcag
     2521 ctgaaaacat ataaaaagat atgtatgtat tctttctgaa attaagttca tttctatgcg
     2581 atgaagcatg ttttcaaaat attactcttt cttagattaa acttcagagt attcaaatag
     2641 cggaataagg agccttatcc cgcattactg aatgagactg tgacataagt aaaaaggtga
     2701 cttcaaattc cttcttcgga atttgaaatc accttcttta taattataaa gtatcggttt
     2761 cacagcattc ctgaatgatt cctagaatct taaacgaatg attggagtta tcaacatttt
     2821 ttcactcgtt tttagcgaaa caaaaaatga catttgtcac agcctcatta aattattgat
     2881 tctttttctt tataattgaa ttgtccatat ttaaagtgtc tagtactcta catgaataat
     2941 taattgcttt cactcaaata cttataatct attaacttgt agttgtttaa cttttctgaa
     3001 aaagttagac ccacgtaata atcttcccag ctttctcttt caattaactg atccaatttt
     3061 tgataatcca taaattcatc atgtattaaa aagaataaat aacttgatat tttagcatct
     3121 agcttaatca gattaaataa tgtttgctt
//
LOCUS       DACDIH010000141         3038 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.142, whole genome shotgun sequence.
ACCESSION   DACDIH010000141 DACDIH010000000
VERSION     DACDIH010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.142"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..95)
                     /locus_tag="IV281_002741"
     CDS             complement(<1..95)
                     /locus_tag="IV281_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8781671.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVA"
     gene            complement(107..595)
                     /locus_tag="IV281_002742"
     CDS             complement(107..595)
                     /locus_tag="IV281_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8781672.1"
                     /translation="MFLKVGTGIAYMKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDYEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQEIKMTILDEVHVVNEEED
                     FF"
     gene            complement(672..>3038)
                     /locus_tag="IV281_002743"
     CDS             complement(672..>3038)
                     /locus_tag="IV281_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8781673.1"
                     /translation="VGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKI
                     FEYAIHEQIIEEDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTEGLLATAN
                     NGYLFLDEVHRLSKENQEKLFVFIDTGNYRPVGENVNWHSAKVRFIFATTEKSEDYLL
                     DTFDRRIQISVMLPTFEDRPIRERLELIQLFFQNEADILQKDIIVSKEALSLMLSHPF
                     SGNIGKLKNIIKISCADVYSRTREEPLTIGKNEILIQLNMLESEVESLPIASLLIKKN
                     QKIKTDRIKPFDYDSILEKITQLINEGNFNEKFSSIKNEVQHLVHHIDKDSFLSMSRK
                     LMIQMFRKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRT
                     TYLCNQFLEHLPPLSEKYENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAV
                     VNQLCGNYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIK
                     NDLKGELLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNI
                     IISCMSGLGISDKLKDVFRHHLSDESIEVFTKEYRELRELIDQNDETYFDKTKLVITT
                     SDLPRSFSIPNINVYDILDGDGAANLKHILSSEMKPVEFNRLIQELIKFFSLEGIADR
                     LNFLNPTIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKAEEP
                     NYDMKPDEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT          921 a          655 c          463 g          999 t
ORIGIN      
        1 gctactacta acccggttaa atttagactc tttgtccaag tcatcgctac ttgtccatga
       61 attaatcgtt catcaattcg cactgcttta atcatttttc ttcctcctaa aaaaaatctt
      121 cctcttcatt tactacatgt acctcatcaa gaatggtcat ctttatttcc tgttggcatt
      181 tatttaccag ttcctgaata tcctcctttg ttaattcctt ggttgccaat actatttcga
      241 gcagaattgg cagattaaag ccagacaaaa tagttacagc aatctctttc tccaaaaagg
      301 ttttggtaat cttctgattg acacttccgc caaataaatc ggtaaaaaca aaaatctgat
      361 cctgctcgtt cacctggttt acaaatacat tcaactcctg ttcataatca cactcatcca
      421 cataagcatt aatcaccgta atctcttgaa ccttccccgt cagtaattga atactgctaa
      481 gaaagcctga agctaagtga ccgtgagtcg ctattagaat tttcttcata tggttaattc
      541 ctttctttct gttcactttc atataagcaa ttcctgtgcc aacttttaaa aacattggtt
      601 taatcacgtt aattcacata aaaaaaagtg tctaacctga aaaaatcaga ttagacactt
      661 ttttagacac tttatttttg aatatacggt cctaataatt catagagcag cgaaagctca
      721 taagcattga ctttaaaatt atatttcatc tccattgatt gaaaaatact tctcgaaaca
      781 taataaaatt catcttcatc tggtttcata tcataatttg gttcttctgc tttttctgtc
      841 cgcgccaaga ataaacgttc catcatcaat gccgtatgca tataaagatt tagttttacg
      901 cggccattta aagtgaaatg atagtaattt tcatatttag aaatgactgt ttccacctca
      961 ctcatcacga ttgttgggtt cagaaagttc aaacggtctg cgattccttc caatgaaaag
     1021 aattttatca gctcctgaat caaacggtta aattctaccg gcttcatctc agaactcaaa
     1081 atatgcttca aattggctgc tccatcacca tctaaaatat catatacatt gatattagga
     1141 attgaaaagg aacgtggaag atccgaagtc gtgatgacca attttgtctt gtcaaaataa
     1201 gtttcatcat tttggtcgat taattcacgt agttctctgt attccttcgt gaaaacttcg
     1261 atggactcat ccgataaatg atgacgaaaa acatccttca gtttatccga gatacccaat
     1321 cccgacatgc aggaaatgat gatgttgcta ctttgagaaa agccttcaaa gtattgaaca
     1381 ttgattgaat acttgtctgc tgcatcctcc gcaatctgtt taaatggcgc attcatcgtc
     1441 atcttcaggc caatatcaag tgcaatggag gtcgtcaaat tattcagaag aagtaattct
     1501 ccttttaaat cgttcttgat ctgtgtgtag atttgattta aagagcccat atctacgatc
     1561 agcactagac tatctgtatt cacttgccgt ttcacgaaat tccttgtcga ttcgacaatc
     1621 tctgacacat tactctctac tggcatatcc aacgcttcaa aaacataatt gccgcataat
     1681 tgattcacta ctgcttgaat gctgctagcc gtactatctc cgtgagcaag caacaggccc
     1741 ttcagctcga tggattcatc tacataatca gataaagtca cgacaataag cagtcttaga
     1801 aagttctcgt acttttctga gagcggtggt aaatgctcca agaactgatt acataagtaa
     1861 gtcgtccgag gtagatttgc agctaatgta cgatttatcc cggttaagtc tgtcttatag
     1921 cttggggaaa tgacatagga tctgctaata atttctgcaa caggtccact cgtcgtcaat
     1981 ccgtacgttt ttcctaaaac ggatacccag acctttctaa acatctgaat catcaatttt
     2041 ctactcattg ataaaaaaga atccttatca atgtgatgca ccaaatgctg tacttcattc
     2101 tttatcgaag aaaacttttc attgaaatta ccttcattta tcagctgagt aattttttcc
     2161 aaaatcgaat cataatcaaa cggtttgatt cgatccgttt ttatcttttg atttttcttt
     2221 atcaataaac tagcaatcgg caagctttct acttcggatt ccagcatatt taactgaatc
     2281 aagatctcat ttttcccaat cgttaatggc tcttcccgtg ttcgactgta cacatccgca
     2341 caactgattt taatgatatt cttcagctta ccaatattcc cactaaaggg atgagataac
     2401 attagagaca aagcttcttt tgaaacaata atatcttttt gtaaaatatc cgcttcattt
     2461 tgaaaaaata attgaatcag ctctaaccgt tctcgaatgg gacgatcttc aaaagttggc
     2521 aacataacac tgatttgaat acgtcgatca aaggtgtcta ataaataatc ttcacttttt
     2581 tccgtcgtgg cgaagataaa acgaacctta gcagaatgcc agtttacatt ttcaccgact
     2641 ggacgataat ttccagtatc aataaaaaca aagagctttt cttgattttc tttcgacaaa
     2701 cgatggacct catcaagaaa aagatagcca ttgttggctg ttgccaataa cccctctgta
     2761 tccttctcag cccccgtaaa ggcccctttc ttgtatccaa acagtgttgc cgaaagcagt
     2821 tcgggattgt ccgcataatc ggcacaattt aaaatagcaa atggtgcatc ttcttcaata
     2881 atttgctcgt ggatagcgta ttcaaaaatt tttgtggcta aaaaactttt tccaaccccc
     2941 gtggctccat ttattaatac actgagtccg ttaggcggat atttgacagc tgcaatacat
     3001 ttttgaatga cctctctcag acttccgtca tgacccac
//
LOCUS       DACDIH010000142         2830 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.144, whole genome shotgun sequence.
ACCESSION   DACDIH010000142 DACDIH010000000
VERSION     DACDIH010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.144"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1..648)
                     /locus_tag="IV281_002744"
                     /pseudo
     CDS             complement(1..648)
                     /locus_tag="IV281_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(970..1191)
                     /locus_tag="IV281_002745"
     CDS             complement(970..1191)
                     /locus_tag="IV281_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781674.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(1289..1534)
                     /locus_tag="IV281_002746"
     CDS             complement(1289..1534)
                     /locus_tag="IV281_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781675.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1774..2388
                     /locus_tag="IV281_002747"
                     /pseudo
     CDS             1774..2388
                     /locus_tag="IV281_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          915 a          510 c          431 g          974 t
ORIGIN      
        1 agtagacacc gaaaagccga agagcgttcc atttcttcgg ttctttttat atattcctcg
       61 aatggtctcc atgcccttaa tcgtggaaga ggctgtacgg agactttgat aaaatttatt
      121 tcgtcgttta ataggtcgat ggtcttgttc tattaaattg ttaagatact tcacagttcg
      181 gtgctctgtc ttagtatata aacccacact ctgtaacttt ctaaaggcgg agccaagaga
      241 aggtgcttta tcggtcacaa ttgctttcgg ctcaccaaac tgtttatgga gtcgttttaa
      301 gaaagcatag gctgcttgcg tatcccgttt ctttcgtaac cagatatcta aggttaagcc
      361 gtccgcatca attgcacgat aaagataatg ccaacgtccc ttaattttga tataggtttc
      421 gtccattttc catgaataga aggattgtct atttttcttc ttccaaagat aatagaggac
      481 tttgctgtac tcttataccc aacgataaat cgtagtatga caaacattta ttccacgatc
      541 atataacaat tcctgaactt cacgatagct tagattgtaa cgcaggtagt aaccaacagc
      601 gacaataatg acgtcttttt tgaattgttt gcctttaaaa tgattcatta ctctgtcctc
      661 tctgtctttt ttctcaattt tacactaaaa tagatttttt ggaaaacttt gcaacagaac
      721 cgtttttact atatacttct tcccagaact tgtccgtctc cttcaaatta tctctacctc
      781 tcgagttcat tgggtggttt gcccatttat taaacatgga atcacctttc tcatactcat
      841 atatttatgt cctatttcaa ttatatttat atatgcttaa ataaatttgt atcctgaaat
      901 ttattgtaat atgaagctgt attttacata tagtaaccaa agagttaata aaataaaaag
      961 agcgaaccat tatagatcag ctcttttttc attgctgttt ttaatcttat ctagaatcac
     1021 ttcaaatgtt tcaaaggtat ttacatctaa ctgacataac caaagcaagt acttcttttc
     1081 tttgtaagtc caatcaatat tacggtaaga ggacattaga ttatggtacc ttgctctttg
     1141 ttcaactgaa gccaattccc agcgtttttc aaatattttt tcttcttcca ttattgatcc
     1201 ctcgcaagac gctacctcaa acttaaataa tgggcagcga aagtctaatt ccttagatct
     1261 aagtatagca aacaaatatg aaaaaaactt agaaggtagt aaaagttctt ccgtcttttg
     1321 ctttgcttct attcttaatg tgtttcgtta tcggtgccct acaaagattt tcattaatta
     1381 cacactcatt tattgttgtg tattttctga catttctctc ttgatcaatt attttaattc
     1441 catgggcatc ctgatagttc ataggagagg agcggctatc agaaatagtt gcacctatct
     1501 cttgaacttc ttttacaact tctggaaaat ccatccatcc tgcttcagct gtcaaaagta
     1561 ataatctata atatttatct ttccttttca atctaagaaa tcgtcacccc attctttttc
     1621 ttctattata aactatttaa cttttagaat tcaaaaaccg tttgtctatt tagagtttac
     1681 cctaaataga caaaaatata acattgaaaa atttagagtt ctaacgtatg attagggtgt
     1741 attataaacg ttatagaaaa gtagggaata aatttgaaaa ttgcttatat tagagtttca
     1801 tccatcgatc aaaatgaaca acgtcaaatt gaagaaatga aaaagtttgg cgctgaaaaa
     1861 atttttatcg aaaaacaatc tggagcgacg attagtcatc ggccgatttt ccaggaagct
     1921 ttagattttg tcagagagca agatattttt attgtagaag ccattgatcg acttggacgc
     1981 aattatgacg agattatcga ttcagtgaat tatctaaaga agaagaatgt gcgacttatt
     2041 atcaccagtt taccgatcat ggctgaagca attggaaatc ctctcctaga taaattcatc
     2101 aaagacttaa tcatttaaat ctcagccatg attgctgaac aagaacgtac tgaatcgaaa
     2161 cgacgccagg cacaaggaat taaaatcgct aaagcaaacg gcgtctataa aggtcgtcct
     2221 aagttataca gtgcagacac caaagatcct caacgtcgcc ttgtctatag gagtatcgtt
     2281 caggatcttg agaatggggt tgctatctca aagattgcaa cagactacaa tgtgactagg
     2341 caaacaattt accgaataaa aaaagaaatc gaccaactaa ttgtatgatt tcttttttga
     2401 atcacgtaat tattaattag ctttgtcatc aatacagaca atcattttag attcatctag
     2461 ctaagttgat tacatttatg ttaaaaagaa tttagttaac ataaatataa ttgatgcata
     2521 aatcgtcctt atttaaaggc atttaacatt gacaacttag tataatgtat cttatgtaaa
     2581 ctaataagat ttgaatttta ttttatacac actcgataat agatcaattt tatggtcccc
     2641 cgcatttacg attgcacaag agaaacataa agtcgaccta ttagcatttc ttttcaattc
     2701 aaacattaaa aaagtttata cctctgctac taaaaaatga aattatctat ttaaaattta
     2761 aaacggtaaa tctgattcat ccaaattgga aaatggatca gcctgtattg gagctttgta
     2821 tttctccata
//
LOCUS       DACDIH010000143         2796 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.145, whole genome shotgun sequence.
ACCESSION   DACDIH010000143 DACDIH010000000
VERSION     DACDIH010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.145"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            121..1458
                     /locus_tag="IV281_002748"
     CDS             121..1458
                     /locus_tag="IV281_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP8781676.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIAIYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDDCATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            1481..2008
                     /locus_tag="IV281_002749"
     CDS             1481..2008
                     /locus_tag="IV281_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781677.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            1995..2558
                     /locus_tag="IV281_002750"
     CDS             1995..2558
                     /locus_tag="IV281_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781678.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
BASE COUNT         1043 a          374 c          609 g          770 t
ORIGIN      
        1 tttattagct tttcctttct attaatttat gttattatat tatcataata acataaaaaa
       61 gtacactttt tgggggcgta gttattgaaa aaatctaaga gaaagattgc cacattgctt
      121 atgttaatgg tgcaatttac agggattgca atgagtgtaa tcacaataaa tccgcaagtg
      181 atacatgctg atgggatagc tatttatgat gcacaattta actgtactac tgaaggtagt
      241 gggtcaggaa cagatagcga tggaaacgat actggaagtg gcgctgtaac aggtgatgta
      301 gccaaaaata ttgaagatat ttacaaatac atgaatggag aatatggctt atcaggtgct
      361 agtattgcag gtattttagg taactggaaa caagaatcag gtattgatcc tcgagcagta
      421 gaagggtatt acggagatgg acagagagaa gttgacttca tcaaacaatt tactgatggt
      481 ctgatgggta ttggctatgg tcaatggaca gctgggcgac atacagcatt agttgattgg
      541 tctaagaaaa ataatggcgg ttcttggtgg actacagaag cccaattgga ctttatgttc
      601 aaaggtgatg ggggattcgt aggaatatta aaagattatg ctttaaatgc ttctgatgat
      661 gtcgtagaaa atgcagtaac ttttcataaa aactgggaaa tttcagctga ttccgaatca
      721 gctgtaatag ctgtacgtgg tgaaaatgca aaagaaatct ggaagtacat gaaaagcaag
      781 ggtatggacg gtgcaaaaga tgaaagtaag atcaataaaa tatccggagg ttcaagtacc
      841 ggagatggat cgaataattc atcaagttca gcatcaggaa cagtagaaga tgactgtgca
      901 actacggatg acgctaattc tggttctgga tcaattggga aagaaggaaa aattaacggt
      961 aaaaaaggtc aagtaatagc gaatatgact tatgaagaag ctttgaaaaa atatgggaaa
     1021 gcgatcacgc taccaaaatt caaagaacca gattggtcaa cttcaccttt taacggaata
     1081 tatcaaggtc agtgtacaga gttgacgtgg gcttatatga gccaattata cgatgggaat
     1141 caaccgacta ctggaaatgg tggttatgta tgggaaagct acaaatcggc aggagcaaaa
     1201 ataacgaaca aaccaatcgt aggttatggg tttagtgctt cggatggatg gttatgggct
     1261 gttggagatg cagggcatac tggtgtagtt atagcggtgt ttgaagatgg atcatttcta
     1321 tgtgctaact ttaatgttcc accaacccaa gctccaacaa ggggagtttc ggtaacgttg
     1381 attgatggag tggacggatc agataatgtg catttcttta gcggtgtagg aaaagctaaa
     1441 tttgaaatcg aaaaataaat tggaatgaag gacgatatag atgaatagta aagtgaaaga
     1501 tacaattatc atttctttgc tttctggtat gttattagca ctgcttgttg gaatcctatt
     1561 cttcaataag cagaatcagg acatagaagc tgaaaaacac agtgtgcata ttgaagataa
     1621 gctgacattc tttaaagagg ttaaaccaaa tgaggtggat ggattacgga aagatttaga
     1681 aaatgatatt caagaaatgt tgaatggatc agttcaaaat aagtttgcag atcaatcagg
     1741 tgaagaattt ttgagaaaac tatttattgt aacaggtaaa ccaccaatta ctgataagag
     1801 tagtgagaaa gaaaaaatcg agtactataa gccttttact tttaagctaa caaactttgg
     1861 aattaagaca tctagtagtg ggttgtatga agcattgacc gattttgaag ttcaatacaa
     1921 tggaaaaaaa gtgtatgata ccacattaac catcacacta aataatgata agaaagtgac
     1981 tgggggtgct ttttatgcaa aatcatgaaa tcactactgc taaaaaggcg atcggaatac
     2041 tttccataat tgtgatcctt tgttgtgtgg tactatttca aaagaaaaat gcttatgata
     2101 ataatgtcaa agaattggaa gaccttaaaa gtaaacaaat cgaagtagtc aagaataacc
     2161 agaaagtgaa agctatgcag gaagctcaat acaaagaatt gggaaatgct gaaattaagg
     2221 aacaaagtag gaaattcttt aatttatttt acaactggaa ttcatgggaa aaatatacga
     2281 agaatatgaa ggaaatacaa aaagaatttc ctcagatagt aaagtctagt cctgttgata
     2341 tttctggcac gatggtaggc actggtaata gtccaatatc tagctatact gctgattact
     2401 atacaacaaa tcaaaaaggt aaaatggttg aattgattac acagacaaga aatactccaa
     2461 caacagcaac aacaacaatt tggcgtggag taacaagcat aggagaaaat caattgtttt
     2521 atttggagga tttagatagc tatgtaagag cagagtaaaa taaaaaaaga gctaacggta
     2581 ccaacgttag ctctttgtgc tgacgatgat aacaaaacct accgaaaaag gattgtttca
     2641 tcacaaacac aaacagaaaa gaattgataa aaaacccgaa cctcagaatt tttattaatc
     2701 aattcattta gattataata aattgagctg aacattacaa tacgtatatg ttgtaaaacg
     2761 taaaaaaagt caaaagtaaa tactttctta aaaagt
//
LOCUS       DACDIH010000144         2728 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.146, whole genome shotgun sequence.
ACCESSION   DACDIH010000144 DACDIH010000000
VERSION     DACDIH010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.146"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..142)
                     /locus_tag="IV281_002751"
     CDS             complement(<1..142)
                     /locus_tag="IV281_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8781679.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
     gene            complement(183..437)
                     /locus_tag="IV281_002752"
     CDS             complement(183..437)
                     /locus_tag="IV281_002752"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8781680.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(548..802)
                     /locus_tag="IV281_002753"
     CDS             complement(548..802)
                     /locus_tag="IV281_002753"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8781681.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(995..1270)
                     /locus_tag="IV281_002754"
     CDS             complement(995..1270)
                     /locus_tag="IV281_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP8781682.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1242..2195)
                     /locus_tag="IV281_002755"
     CDS             complement(1242..2195)
                     /locus_tag="IV281_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8781683.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
BASE COUNT          857 a          482 c          398 g          991 t
ORIGIN      
        1 attgctttac ccatttgtca aatgctgaag ccgttagttc atattctcga atgatttcta
       61 cacgtggctt tccagctaag taaagattga cgatttgttg cttgaattct tgtgaaaaag
      121 ttcttcgtgt tctcttagac ataaaaattc ctcctggtat gttttcttct agtctacaca
      181 ccttaatttt tctgtctagt taattgtagc ctatccatga tcggtttatt agacctttga
      241 ggtctatatt ttttaactac aatcgacctt aaatttaatt gtttcattag cttttggaca
      301 tgtttaagag atactgaaat cccttctttt aataaaactt gatggatctt tgtggcacca
      361 tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct ttttcaactt attatttctg
      421 atttctgtag ctgtcaacgg tttattctta taaaaataat agacacttct agggatttct
      481 aataaccgac acaacaaaga aacagtgtaa tccttacacc atttttcaat aaattgaaca
      541 agcgaattta ggttttccga gagaatatgg ctgcggcttt ttttaagatt tcaagttcct
      601 catttaaacg agcattttct tttctcagtt cagctacttc ttttccagtc agtcctgtgg
      661 actgatcagg taaatataaa ttcttccatt tgtaaatcgt tgcttcagat aagccatatt
      721 ctttcgctaa ccctcgaaca gaacgtccag tttgattcag ttcaacaatc atttgtttaa
      781 aattttcttc atatcgggtc ataaaaattc atcctctcgt agataagttt aatttattct
      841 tgtctctacg aaaagctgtc taagatttga tactaacacc aaatgttgta tacccccaaa
      901 caatacacat attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa
      961 atatatttac tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata
     1021 taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt
     1081 catacatagg aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat
     1141 ctacttgaac tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa
     1201 tatcatcttt aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta
     1261 ataatgccat ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca
     1321 gcatatttag gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt
     1381 ctttcttcta attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa
     1441 tcatttaatt ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg
     1501 cttttaaaaa tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga
     1561 gaggaacgtc gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct
     1621 tcaaaattcg tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt
     1681 gcgtaccgtt ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga
     1741 accacattat ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat
     1801 tcttctaaag gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac
     1861 aagtattcat taagttcttc agatccagga gagccagcca taatatataa atgtggactt
     1921 acttgagtaa tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca
     1981 aagctagtaa tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa
     2041 tcaattagca acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg
     2101 tctgtagtct taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta
     2161 tctgatgttt taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa
     2221 atatatttag tatacttcat atacttaata tattcgatat atctaatata ctccaaattg
     2281 ataagaattc agactttata aggaatatag tggatatact aaatatattt gctatattca
     2341 gtatatcaga tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag
     2401 agtgaaaaga ggtaaactta attctataga aaccacagat aattcactat ttacaaagta
     2461 gcaaaagcag tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat
     2521 tgattttagc agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt
     2581 ctcaagaata tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc
     2641 cgattgcctt tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt
     2701 gtacttaaat taaatattct taaacact
//
LOCUS       DACDIH010000145         2664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDIH010000145 DACDIH010000000
VERSION     DACDIH010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.147"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..2145
                     /locus_tag="IV281_002756"
     CDS             <1..2145
                     /locus_tag="IV281_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAP8781684.1"
                     /translation="IGYCMDSELPSPTGSGAGSLKYKGAGSDEFYRMFKGGFPSKTAK
                     ELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSHPNHTAAETARVKKAFEKIYDYAKN
                     GKDTPNTEFSITASKTADEGKYHTFTYKTASNKTGNAKLTFTSAKPAGMKIYDADGKE
                     ITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVSTTNPFTFDGRGVYQDAVVMITTSE
                     TKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAEFTLTDNNNAKSVKTTGDDGLATFD
                     LQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQEFSYTATNTIKKGKVKVKKVDQDG
                     KPLANAEFTLTDDSSQTQAEKTDENGLASFDIVANKTYSLEETGVPTGYTGVFKQEGI
                     TVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPLANAEFTLTDEKGATQVEKTDENGL
                     ASFNIVANKTYSLEETGVPVGYTGAFKQEGITVKDDGQTFEYTATNNLKKGVVKVHKV
                     DQDGKPLADAEFTLTDEKGVKEVKKTDATGLATFNIIANKTYNLEETKVPTGYTGAFK
                     QEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQGKALANAEFTLTDDKGVKVVQKTD
                     SNGLATFNIVANKTYKIEETQNPNGYTGKWSKEGITVADDEQTFEYTVINHLVPETVR
                     TGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            2194..>2664
                     /locus_tag="IV281_002757"
     CDS             2194..>2664
                     /locus_tag="IV281_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781685.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDN"
BASE COUNT         1033 a          431 c          547 g          653 t
ORIGIN      
        1 attggatatt gtatggattc agaattgcca tccccaacag gaagtggagc cggaagcttg
       61 aagtacaagg gagctggcag tgatgagttc tatcgtatgt ttaaaggtgg ttttccatca
      121 aaaacagcaa aggaactggg tgcaggaaat gatacagaag catggtatgc aacacaatta
      181 gtttcatggg ttttagccgg aaactttaaa gtttcacaga ttgtatggtc acatccaaac
      241 cataccgccg cagaaacagc tcgtgtaaaa aaagcatttg aaaaaattta tgactatgct
      301 aagaatggaa aagatacgcc caacactgag ttttctatca cagcttctaa gacagctgac
      361 gaaggaaaat atcacacttt cacatataaa acagcatcaa ataagactgg taatgcgaag
      421 ctcactttta catcagcaaa accagctggt atgaagattt atgatgcaga tggtaaagaa
      481 attactaaca acactgtgaa attaaattca agttttacta ttaaagtacc tgtgactaca
      541 ccaagtggta ctttgtcatt taaaggtact gctaatgtta gcacaaccaa tccttttact
      601 tttgatggac gaggtgtgta tcaagatgcc gtagttatga tcactacttc agaaacaaag
      661 gactctaaat ctttgagtgc taaatggaca agagctgttg gtcaggtaac agttaagaaa
      721 gttgatcaag atggaaatgc tttaaaagga gcggagttta ctctaactga caataacaat
      781 gctaaatctg ttaagacaac cggagatgat gggttagcta cgtttgattt gcaacaaggc
      841 ttaacataca gcttagaaga aacaaaagtt cctgaaggtt atcaaggttc atttaaaaag
      901 gatggtatta ctgttaatac cgatggacaa gaatttagtt acactgctac gaacacaatt
      961 aaaaaaggta aagtaaaagt taaaaaggtt gaccaagatg gaaaaccatt agctaatgct
     1021 gaatttacct tgacggatga ttcatcccaa acacaagcag aaaaaacaga tgaaaacgga
     1081 cttgcaagct tcgatattgt agcaaacaag acctacagcc ttgaagagac cggagtacca
     1141 actggttata caggcgtatt caagcaagaa ggaattaccg ttaaagatga tggacaaact
     1201 tttgaatata cagctacaaa cactttgaaa aaaggtttag taaaagttca taaagtcgat
     1261 caagatggaa aacctttggc aaatgcagag ttcactttaa ccgatgaaaa aggtgcaaca
     1321 caagtagaaa aaacagatga aaatggactt gcaagcttca atattgtagc aaacaagacg
     1381 tatagcctag aagaaactgg agtaccagta ggttacacag gtgcatttaa gcaagagggt
     1441 atcaccgtta aagacgatgg gcaaactttt gaatatacag ctacaaataa tttgaaaaaa
     1501 ggtgtagtaa aagttcataa agtcgatcaa gatggaaaac ctttagcaga tgcagaattc
     1561 accttaacgg atgaaaaagg tgtaaaagaa gttaaaaaga cggatgcaac tggtctggct
     1621 acgttcaaca ttattgcgaa caaaacttac aacttagaag aaacaaaagt accaacaggt
     1681 tacacaggcg cattcaaaca agaaggaatt accgtcaaag atgatggaca agtctttgaa
     1741 tatactgcta aaaacacttt gaaaaaaggt gtgatcaaag ttcataaagt ggatgatcaa
     1801 gggaaagcat tagcaaacgc agaattcact ttgacagacg acaaaggtgt aaaagttgta
     1861 caaaaaacgg acagcaatgg cttggcaaca tttaatattg ttgccaacaa aacgtacaaa
     1921 atcgaagaaa cacaaaaccc taatggttat acagggaaat ggtctaaaga aggcatcacc
     1981 gttgctgacg atgaacaaac ctttgaatat acagtaatta atcaccttgt acctgaaaca
     2041 gtaagaacag gcggaatcgg tacatcagcc ttccttcttg tatttctaat tgcaggattg
     2101 agtatggtgc tactgtatta tcgagaacag cacaaagcaa aataaaaaag tgttaatgga
     2161 aaagaagtaa atatgaaaaa agaaggctga cgtatgaaat caaaaaaaat tttttcaaat
     2221 ttaatgctat ttggtacttt gctaggagtg ggattgggcg caagctcaat ctcttctagc
     2281 cttggactag gggggcaagt agcatatgca caacaaaact ataattatca aggaactgga
     2341 acgattcata tccataaagt aacaaatact aatggggatg ggaaccctgt ctcagatatt
     2401 gataacaaag ggaaagaaat agctttacca aatggtgtaa aacctcttga aggcgttgag
     2461 ttctcttatg tgaaacttac taaagatgaa gctaaatcct tgggatggac ggatagtgat
     2521 actacaaaag gtcaacatat tgatgatgct aaagcacgat tattactaaa caaaaaatac
     2581 agtgaagtaa gtagaatgaa gactgattca caagggaata tagacatacc tatacaagta
     2641 aatacagacg atttaacaga taac
//
LOCUS       DACDIH010000146         2611 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.148, whole genome shotgun sequence.
ACCESSION   DACDIH010000146 DACDIH010000000
VERSION     DACDIH010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.148"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..493
                     /locus_tag="IV281_002758"
     CDS             <1..493
                     /locus_tag="IV281_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781686.1"
                     /translation="IKTDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIAD
                     KSKATEFVSGSDGKFQIEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPT
                     RNDVPFVVSNDDASSEVTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQ
                     HKA"
     gene            568..1425
                     /locus_tag="IV281_002759"
     CDS             568..1425
                     /locus_tag="IV281_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8781687.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKIILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            1415..2494
                     /locus_tag="IV281_002760"
     CDS             1415..2494
                     /locus_tag="IV281_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8781688.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDGGSFDIGEAEGKVNPYTGDVFSKNQT
                     KDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLPLSGKGHQTVI
                     AGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQNSTLTDIPDL
                     DMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDSEYVSDKTETV
                     TSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTGITNKHQKMLL
                     VIWTVIGALASLNVLILLIKEFRKA"
BASE COUNT          963 a          392 c          509 g          747 t
ORIGIN      
        1 cattaaaaca gatggagata caagcaaagc attgagtgga gcaaaatttg tagtgaagaa
       61 caaaattgat ccgaataaag ttacttacct aaaacaagat ccaacaacga aagctgttac
      121 ttggattgca gataaatcta aagctactga attcgtttcc ggatcagacg gtaagttcca
      181 aattgaaggc ttgcaatata cctatgatgt agattttgat aaacaaggta atccaacaat
      241 cacagataaa aaaatcaatg attatgctct tgaagaagtt gttgctcctt caggatatac
      301 accaactaga aatgatgttc catttgtggt atcgaatgat gatgcttcta gcgaagtaac
      361 agacatcaag aaccttaaaa cacctgaaac agttcgtaca ggtggaattg gaacagtagt
      421 atttctagtg gttgcaggtg tggcaggaac agggctatat gttgtctaca aacgagaaca
      481 acacaaagct taatgaaata aaattcccta gagtgttttt ggctctaggg tttcttttta
      541 aaaagtgtgt gaaaagtagg tatgagcatg aaaaagaaga atatcaagtt gatcatctta
      601 aaaattggga tgttgctatg tttaggagta ggtctatatc ctgtaattac tcaggcttat
      661 acctactacc aaactcaaca aacactaaac tcctataagg aaaaatatga tgaagcttct
      721 gaaaaggaga atgaagaagt cttcaagaga tattcagaag ttaataggaa acgaaaagac
      781 acagaacaaa cctcagtcac gattcctgaa gataataaca atattccgtc aaatggaaat
      841 cttcagaagg ataaattagg tgaattagtg ggtgaaattc ttataccaaa gattaatgtg
      901 gatttgccaa tttatgatgg tgtaaacgat tttcaactat cagcaggagc aggaatatta
      961 accggaactg attatccaac tggtgggatt ggaaaccatt ctgttatatc agctcattcg
     1021 ggcattccta ataaaaaata ttttacagat ttagagttgc tcaaacttgg tgataaattt
     1081 tttattgata tttcagggaa aaagcttgct tatcaagtag atcggataaa aataatccta
     1141 ccaaccgaat ttgaggattt gcttcctgtg gcaggaaaag attatgtaac acttatgaca
     1201 tgtacacctg ttggagataa ttctcatagg ttactcgtta gaggggttag cataccttac
     1261 caaaaaagta ttgttgagaa agaaaagaag aaagaagatt taaaggtaaa ggcgcagatg
     1321 aactggtatc ctatcgtagt tgtaggggtt ttattaataa tagctgtgat agtcattatt
     1381 aaaaaaatag tagcaaagag acgaggaatg aaaaatgaag aataaaatac tacttttttt
     1441 gactcttatt tgcactttgg ttttaacaat ttatcctgca aatcagctct atcatcaatt
     1501 caaaacaaaa gagaatgtgg cggaaatcca agagaaaatt gagaaaaaac aaactccttc
     1561 aaaagaggaa gtggcaaaaa aagtcgaaca aataaaatcg aacagcggta accaaggatt
     1621 agacaatact cagaaaacag acggcggatc atttgatatt ggtgaagctg aaggaaaggt
     1681 caatccttac acaggagatg ttttttcaaa aaatcaaacg aaagattttg aaccatctac
     1741 ctattcaggg gcggttggtt atgtctatat tccaaaaata aatgtgctag agcttttgtt
     1801 tctaggcgct tcacaggaac atttatatag aggtgtggca cagatgagtg ctactagtct
     1861 acctttatca ggtaaaggtc atcaaacagt aatagcaggt catcgtggcg gttatacgtc
     1921 accacaattt ctttatgttg atcaattagt agccggagac cacatttata tagattatct
     1981 tggtgaaaaa gcagtttata cggttttccg aacagacgtt attagaaatt acggtcagaa
     2041 ttcaacactg actgatattc ctgatttaga tatgttaaca ctttatactt gtcatccata
     2101 tcctcagaac tacgatcgtt tattagttag aagtctaaga gatgaaaaag caaaatacat
     2161 cacgccacaa gtaacagttg gcaataccgc atacgctgat gccattgata gcgagtacgt
     2221 ttcagataag acagaaactg ttacatcttc taatgattta tttgaggatg ttgatcgtgc
     2281 caacaaggaa ttagatggaa ataatgatct taatattccg gaacaaggta gcgtagagat
     2341 gcaagacaga acatttgctg aaaaggcaag ttcaatattc acaggaatta ccaataaaca
     2401 tcagaaaatg cttttagtca tttggactgt aataggagca ttagcaagtc taaatgttct
     2461 tattctttta atcaaagagt ttcgaaaagc ataatgaaga ttataaaaat tgatagagta
     2521 cagtaaaaag ggattgatac tattattgat ctctttttta ttttgaatag agctagattc
     2581 acttttaaat aaaatcaaaa ctgatcttaa t
//
LOCUS       DACDIH010000147         2579 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.149, whole genome shotgun sequence.
ACCESSION   DACDIH010000147 DACDIH010000000
VERSION     DACDIH010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.149"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            42..581
                     /locus_tag="IV281_002761"
     CDS             42..581
                     /locus_tag="IV281_002761"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781689.1"
                     /translation="MNKRRILWLIAGMYLCIAVSGFTICRIINQLKNAIVYLGEIGYL
                     EDLKAGLQIMSWILFVLTVALVILTCENFSTIKKQIISNHNQETVKSGLEVDPEEEVT
                     EQAIKEQEEIDKKEIMSIYLSSGESFFHEIDSLKLSSQEIIDRWQNMSLDGELIVEDS
                     EGWSAYKKDEIVAITILFV"
     gene            1232..1285
                     /locus_tag="IV281_002762"
                     /pseudo
     CDS             1232..1285
                     /locus_tag="IV281_002762"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1289..1888
                     /locus_tag="IV281_002763"
     CDS             1289..1888
                     /locus_tag="IV281_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP8781690.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLQSNIVSMGLSGGIFGWIALFAIAYIHFDSKEHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            1904..2380
                     /locus_tag="IV281_002764"
     CDS             1904..2380
                     /locus_tag="IV281_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781691.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMHE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
BASE COUNT          964 a          332 c          491 g          792 t
ORIGIN      
        1 cacatactct taaagtgtct tttcaacgga ggataagcta aatgaataaa agaagaatac
       61 tgtggcttat tgcaggaatg tatctttgca tagcagtatc tgggtttact atttgtcgga
      121 taataaatca gttaaaaaac gcgattgttt atctaggaga aattggttat ctagaagatt
      181 taaaggcagg actacaaata atgagttgga ttttattcgt tctgacagtt gcgttagtta
      241 tattaacttg tgaaaatttt tcaacaataa aaaaacaaat aatatctaac cataatcaag
      301 agactgttaa atcaggtcta gaagtagacc cagaagagga agtaactgaa caagcgatca
      361 aagagcagga agaaatagac aaaaaagaaa taatgagtat ttatttatct agtggagagt
      421 cattttttca tgaaatagat tcgctgaaac ttagtagtca agaaataatt gatagatggc
      481 aaaatatgtc tttagatggg gagttaatcg ttgaggatag tgaaggttgg tcagcttata
      541 agaaggacga gattgtagct atcacgattt tgtttgtata gtaatatata ccactagata
      601 atctgaatat gttgatatgc tagtgacgta ccaaatgtgt tcaatagagc cgtggtcaca
      661 ggcagaaatt gataaaatag taaaatttaa ccgattatca aaatttaacg ttttaggaaa
      721 taccttttaa aaatcacaat acacaaacag aaaaatacca aaaaattaac aatgaacgta
      781 ggtaagaaca tgacaataaa tgataataac ctcaaaaaat ataatgctct tgtccaacgc
      841 tatatcgaaa acaaaataac gatgcaagag gtcgctgatg aattaggcaa atcaaaaagc
      901 ctagtatcac acgactttaa gcacaatgtt tttccgaacc cgattgagct gaggaaaaaa
      961 tttcaaaaga gagtttatca agaaataggg aagtggattg atgaagggaa tgcaattgac
     1021 cctttaattg aaaaatatcc ttttttaaaa agcaaaaata aaattcaaag gttaattcgt
     1081 caaggcgata ttcaagctaa agaacctgtt ttgacaacca atgatttggc taaaataaat
     1141 cttcataaac aaattattga agactttgaa aaaaatcttt caacaactga aattttaaaa
     1201 aaatacaata ttccgagagg aaagctaacg tcgactcttt ttagagaata tggagaatcg
     1261 gcagttacta aagcaaagga agtagcgaat gagagaaaaa aacaaaaata gtagtcgata
     1321 cttagtagcg actgtagttt ttgtttgtgt attgattttc ctgtttgatt ttttacagat
     1381 tggaaaaagt ataattggaa atacattgag tgcaaaggaa atggttgctt taggtggttt
     1441 gtatgaagga catttgttcc cgaatatatt catttctatc gtttctcata ttaacttgta
     1501 ccatttaatt tctaatttat tgttcttagt agttcttgaa gaagcactga atgatcagta
     1561 tgagtggtta acattattga tcgttttaat catatcagga tttctaggaa gcttagttcc
     1621 ttttctgcta caaagcaaca tcgtaagtat ggggcttagt ggaggtattt ttggatggat
     1681 tgctttattt gcgattgcat acatccattt tgatagcaag gaacatttat tactaagtgt
     1741 ttttgtatta ttgataatcg gcattttatt ggtatctact tttgaatcag gcgaggcaaa
     1801 tgtcttgtca catgtaatcg gatttgtctg tggaatgatc ttgggattta ttctaaagaa
     1861 cgtttcattg atcaaacaaa agaattaaaa gggagtgcta aaaatgccaa aaagaaaaga
     1921 aaagaaaatt ccagtgtttt tgtctgtctc agaaatgtta tcacttcaaa tactgttaag
     1981 aaaaaagatt gaaacaatag acgaagaagt tagaaataaa agggctatgc acgaacaacg
     2041 tgaagcgcta agagaacgaa tccactattt tgatgatata cacactaaaa tctatgaagc
     2101 acgtacaaat cacgaagacg aaagtaaatt aggattagct caacaacgga tacttgaatg
     2161 gcttaatacg aatactgatc aagaaccgtt aagagtgatt agtaatcttt ttgaatttgg
     2221 aacgaagcat tggtttaatg ctgtagagaa ggatgctgac ttacaagaag cgtttgatac
     2281 gctaagtaag gatagcaaat ccatgcgaga tttattgatt acttatctaa atgagcatga
     2341 gtttgaacaa acgaaaacca aaaagaaaaa taaagaatag aaagttgtga tgaatcatgc
     2401 tattaaccct gttaggttat ttatgtttaa ttcttgcttt ttttggggca atcgctataa
     2461 ttattggtca atttcaaaca aataatccga ttatgcgtaa cataggcatt ggaattgtga
     2521 ttgtaacctt tattgtgttt aatgccacta agatttttgg aagtaacgat aaacaattt
//
LOCUS       DACDIH010000148         2559 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.150, whole genome shotgun sequence.
ACCESSION   DACDIH010000148 DACDIH010000000
VERSION     DACDIH010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.150"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(30..446)
                     /locus_tag="IV281_002765"
     CDS             complement(30..446)
                     /locus_tag="IV281_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781692.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            839..2086
                     /locus_tag="IV281_002766"
     CDS             839..2086
                     /locus_tag="IV281_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781693.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            2354..>2559
                     /locus_tag="IV281_002767"
     CDS             2354..>2559
                     /locus_tag="IV281_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781694.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPN"
BASE COUNT          878 a          408 c          503 g          770 t
ORIGIN      
        1 agggcttgac tagcctttgc tccatttttt tattttacaa atgaatacag ttcgtcgttt
       61 ttaccaagaa tgattgtttg cccaactttg agattgtcag gtgatccttc agcggtttgt
      121 aaggagaacg ttccattgaa cttatcggat accgttatag gtagctttaa ttctgatcct
      181 tcctcatctt tgagaattgc tgtttttcgt tcatcaaaat aaacagtctt tattaaataa
      241 tttttcgtag tgatctcagc agatttgtct ttttttgtag tagtaccagt ttctttagaa
      301 ttagaggtat tttctacgac tattccatct ttggcatttg agcttgtgtt tatttgattg
      361 aatattttca gcgacaagat aacgctaaga atagtcaata tagaaacaag tgtaattaat
      421 actgataatt tccttttttt gttcattttt tcttaacatc cttttttgtt ttgattagtt
      481 agatattcct tatctaacaa gcctttttag gctattaaat gaagataaac cccaaaaaaa
      541 tattgcaatt atgacagtgc aatggtacta ttattataca ataaattttg cgaaaggggt
      601 aggtctaaat cgcaaacaaa aagaaattga acctagacaa atacaacttg tataccgcaa
      661 gggaagcaag tttgattttg ggtaaaggta gaaattatgt tacaaacata atcaactatt
      721 atccaaatcg tttgcctaaa ggttcgttca agaaagtggg tgacaaaacc tatattataa
      781 cggaagaagg aatagaagca ttgcgatcac aaacagaaaa taaaaacaaa aagaaggaat
      841 gaatatgaag agaaattcgc ttggtatggt tttattggca tcagctgtac ttgcggggtt
      901 atcagtacca gtaacctcac atgctgatga atggactgca aacacaccgg atcaaatcca
      961 aattacaaca ggacaatcaa gttatgcgtt caaaaaagga gatactattt ggtcaactgt
     1021 agagaagctt aaaagcttag gttacaagac tactgttgct gagtttgccg aattgaacaa
     1081 tgttgatttg gcaaaaggag aacaacggac tatcccgatt ggacgaactt tcaacatcag
     1141 taaagataca ttaacagtta aggaagcaga tggcgaagta gttagtcaaa ctgttcaaaa
     1201 agataaacct gatcctattg aaaatacgga gcaagagaac aaaaaatccg agactacaaa
     1261 agaatcggaa ccaacagcaa acacttctga taagacagaa acaagtgctt caagtgttga
     1321 tggagcaagt tcatctaata caaataattc tacaagtgag aaagaagcat cacagggaac
     1381 aagtgaaagt agcactacgg aagctgatac tggaacatca acaaataact cacaatcaac
     1441 atcaaatgat gcaaatgaat caaccagtac atcttctagt gatcaatcac aaacaggttc
     1501 gagtgatgaa actgacagtt ctacttcacc aagtaaaccc gaacaaccaa ctaatgttgt
     1561 gctaggcgtt ccatttatta gtcaaggtaa cacaatgctt tgcgaaggaa catctttgct
     1621 agaagcgtta cattataaag gtgttgcaac aaatcaagat ttaatgactt ttgttaaatc
     1681 aatgccacta tctcctgaca ataatccaaa taatggtttt tctggtgaat ggagacacaa
     1741 tgtggatgga acttatcaag gtatgaatcc ggctcctgta gtttcatggg gtgttgctaa
     1801 tggcggaaat gttgtagata tttctggttc atcggtacaa ggattaaaag atgagattgt
     1861 aaatggaaat cctgtagtag cgtgggtaac ttatgccttt gaacctgctc aacataaacc
     1921 aatgttttgg ggcgatgcta tttggaatcg acatgtagta gccgttgatg gatttaaaga
     1981 cggtttttac catgttgtcg atcctgtgtt tggggcaagt tggattaatg caggatcatt
     2041 tgaaacagca tataacgtat ctcaattagc ggtttcggta cgttaataaa agatatcgtt
     2101 tatatactaa gtagttatat agtctttatt tgtccttgct taactactta gaaaacttgt
     2161 tgctcatggt gggtagaact aaaaggggtg atgctatgaa tactagagcg ttgaaacagc
     2221 ttgctttcaa cgaaaaaaat gattaatagg tgtggtgatt ttaacttagg aatgggcgcg
     2281 aaggtcatta ccacaccgac atatcgtggc tcattttctt cgcctaactg gtataaagaa
     2341 ggagtaacga gagatgaagc atgaaaaaac gataatttta aaatcaaaaa taatttattg
     2401 ttttgcgact ttagtaatga tgttatctac gcttataatg cctttttcag caataagagc
     2461 tgaagcagat gtcacaaaac cgacaagtgc aaaatttata gaaactggcg taaaaacaga
     2521 cggttatatt agggttaata tgccgaatca tccaaatga
//
LOCUS       DACDIH010000149         2450 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.152, whole genome shotgun sequence.
ACCESSION   DACDIH010000149 DACDIH010000000
VERSION     DACDIH010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.152"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            329..478
                     /locus_tag="IV281_002768"
     CDS             329..478
                     /locus_tag="IV281_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP8781695.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            489..2003
                     /gene="dltA"
                     /locus_tag="IV281_002769"
     CDS             489..2003
                     /gene="dltA"
                     /locus_tag="IV281_002769"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP8781696.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            2000..>2450
                     /locus_tag="IV281_002770"
     CDS             2000..>2450
                     /locus_tag="IV281_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP8781697.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRY"
BASE COUNT          786 a          391 c          502 g          771 t
ORIGIN      
        1 ctttatgtca ttacgagtca gttatattgt cggattatcg aggaatgata tacggaataa
       61 gacaagataa taaatagaac taaattttga ataccattta tttggaataa gaggttgtga
      121 cacgattttt gtcacaacct ctttattttt tatatttttt aaaagtacga gaaattataa
      181 agaaaaaagc agtgatttga tgtgctattt ttataaaagc tatgtgacaa aactgttagt
      241 aaaaaaacgg tttctgtaag ctagacacat gtttcttttt gtcaaaatta gttacacttt
      301 gtgtgaggtt atatgaggag ggaatccaat gaacaaatgg cgttctaatc caaaattgaa
      361 ttattgggga aaattcatca ttcgaacaat tttttatagt gcagtactgc tgttcctgat
      421 ttatctgtat cactataaaa atattcaagg aggaactttt atctataacg aattttagga
      481 ggaagagtat ggaaatcaaa acgattattg aagcaatcga tgattgggga ataaatgaac
      541 ctgatcgagt tgcttatcag gcggatgaga cgcatacttt tggtgaattg aaacaagcat
      601 cagacgcact tgcttattat ttagaaaaaa aggtagaagg cgatggacca attgtcgttt
      661 ttggcaattt ggagtttgaa atgattgtct catttctagg ggttgtcaaa tctggccatg
      721 cctatttgcc gattgaagag catacaccca aggagcgtat cctatcgatc tttcgggtgg
      781 ctaaaccatc tatggtcatc agtatcgggg actggataga agatattcct tcggttcctg
      841 tcataacaaa aaaagaattt caagaaatca agaagatacc agtaggattt tatcccagaa
      901 atagcgtaaa aggtacagaa aatttttata ttatttttac ttctggtact actggagaac
      961 caaaaggcgt acaaatcagt catgataatc tagtcagctt tgttcaatgg acgatggaag
     1021 actttgggat caagcctggt atgcatttct tagcccaagc gccattttct tttgatttat
     1081 cggtattttc tatttatcct gcattagttt ctggcggaat gctgaagccg ttaaataaag
     1141 cagttgttca agactttcgt caattattcg cgacattgcc agccttgaag ttgaatgtgt
     1201 gggtttctac tccatcattt atggatattt gcttgatgga acctacattc aatggagaaa
     1261 atgtgccgga attggacatg tttttatttt gcggagaaga attgacgaaa aaaactgcag
     1321 aaagtctact tgatcgattc ccaaatgcac ggatatataa tacttacggt ccaacagaag
     1381 caactgtagc tatttcaagt attcagattg accagcatgt actagatagt tatgaccgac
     1441 taccgattgg ctacgtaaaa aaagacacga aagtttctat tgtccaagaa ggtaaacctg
     1501 tttcaaaagg agaaacgggt gaaatcatta ttgcaggacc aagcgtatca aaaggttacc
     1561 tgaacaatcc cgaaaagaca gctgctgcgt tcttttgtca aaatgactta gcaagttacc
     1621 atacagggga tgcaggtaga ctagatgaat atgggctttt attttatgaa ggacgtatgg
     1681 atcagcaggt caaattacat ggataccgga ttgaactcgg ggatatcgaa cattatctgc
     1741 tccaagacaa ccgaatcaaa caagctgtcg tcgtaccaaa ataccaagga acaaaagtac
     1801 agcagctggt tgcttttgtc gtattagaaa acaaaacgaa acagccggat tttcagttga
     1861 ctaaatctat caaagaacaa ttactagaag ttgtaatgga ttatatgatt cctcagaagt
     1921 tccattatgt cgatctgctg cctcagacag ttaatggaaa aatcgatcga aaaaaattaa
     1981 cagctgaggt gaatcctgaa tgatgttacc tcatatgatc ccttatgcag agccgtttta
     2041 tttttttctt ttagccattg cgcttgtacc gatcgttcta tctttattga tatgggaaaa
     2101 aagattacca gtttatcaat cagcagtgac acttttcttt ttatttgtca gctttggagg
     2161 taattattgg caacagggag cggcgctgat tgggtatgtt ttgtggcaga cgatacttgt
     2221 atggggctat tttgtttatc ggcaaaggaa aaatcagaca ggatggtttt ttgccgccgt
     2281 tattttagcg ataattccac tgtttttaac gaaagtgact cctttttttg aacatggaaa
     2341 agcatcgtta cttggttttt taggaatctc ttatttaacc tttaaatctg tacaaatgat
     2401 catggaaata agagatggca tgatcaaaac atataagcca tttcgttata
//
LOCUS       DACDIH010000150         2447 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.153, whole genome shotgun sequence.
ACCESSION   DACDIH010000150 DACDIH010000000
VERSION     DACDIH010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.153"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(148..2301)
                     /locus_tag="IV281_002771"
     CDS             complement(148..2301)
                     /locus_tag="IV281_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP8781698.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          630 a          548 c          421 g          848 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgttgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accggaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttcttat tttttctaac caagaagtat ttaatatttt
     2281 tttcataaaa tctttcatca tttcctatcc ttccgcctat ttacattcat gcttcattgt
     2341 acccaaaaac ttcttaataa acaagaagta tttaatattt ttttcataaa atctttcatc
     2401 atttcctatc cttccgccta tttacattca tgcttcattg tacccaa
//
LOCUS       DACDIH010000151         2428 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.154, whole genome shotgun sequence.
ACCESSION   DACDIH010000151 DACDIH010000000
VERSION     DACDIH010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.154"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..794)
                     /locus_tag="IV281_002772"
     CDS             complement(<1..794)
                     /locus_tag="IV281_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8781699.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRR"
     gene            938..1714
                     /locus_tag="IV281_002773"
     CDS             938..1714
                     /locus_tag="IV281_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8781700.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            complement(1812..>2428)
                     /locus_tag="IV281_002774"
     CDS             complement(1812..>2428)
                     /locus_tag="IV281_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP8781701.1"
                     /translation="VLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKIGTTASIFDDIE
                     KYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIKMAIASSSPMDT
                     INEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEECLVIEDSYNGIK
                     AGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
BASE COUNT          789 a          401 c          396 g          842 t
ORIGIN      
        1 cgtctaagca agccgtttga gtgctcattt agccctcttt gcgaaggtgt tcctggatct
       61 gcgaaaaaga tggcaatatc gttcgagtta cttatagatc gccagttgga aaattctttt
      121 ccacaatcaa atgtcatgga tttaaacaag tgtttaggca tggatttgaa ccaatcattt
      181 aacctgtttt cgatatcttc ggctcgtctg cctgtcggtt tcaaagtaat aataaccttg
      241 gataaacact caaccaatgt aataacagcg ctcttgtgtt taactcccac aatggtgtcg
      301 acttcgagat gaccaaattc tttagtcagg tgtggataga gactgttccg ttcatgaatc
      361 gttctgcgaa aggcctgttt tccacgcttt tcttgatgat tattcggttt acgtttcccc
      421 ttcataggta acgactgtgt gtcaaaaagg ccttttttaa acatccgata gagtgtacga
      481 acagaacaat caattggaaa ctcgccacgg ccgataatca catcgggtga ccatccttga
      541 gctacctttt cttgaacata tgttgattgt tcatcagata aaacggttga acgtcgtcca
      601 caacgagatt tattttgttt gtattgacgg taataatcta gcactttttt tccttgtttg
      661 agaaacgaat aaacattata aatggtttgt ctagagcgat ttaaactttg tgcgacaaga
      721 gcgacaggtt tctcgatatt gaaataagac tctatgatta ctagttcgtc tgttgtaaga
      781 tgggtgtaag tcatttgtac tcactcctta ttaattcttt ggtcggaact attttgagtg
      841 tagcacaaat gatcttttta gttgtctagc ttaattttac aatcgacgtt ttaataaatt
      901 gtgatataat gaagtataac tttttaggga gtgtactatg aattttcaag ataatagacc
      961 attatattta caagtagaag cttctatacg taacgatatt cgtgaaaaaa aatatcttcc
     1021 aggagagcaa ttacctacag aagaagaatt atgtaaaatt tatggagtaa gtaagattac
     1081 cattcgaaaa gcttttaaac ttttagcaga aagtggaaca gttgaacgat taagggggaa
     1141 agggaccttt gtaactcaaa aaaaagaaag tttaaaatta gctggagttc atggatttag
     1201 cgattctctt tctacccgag gacataaaat tagatatact gttttacatt ctaaaattat
     1261 aaaggctgat gagtttttag ctaataaatt acaaatcaat gtaactgata gagtttacaa
     1321 tattaaacgt ttaatgtggg aagacggtgc cccgatggga attgataatt ttttcgcatc
     1381 agaagaaaaa tttccaactt tatttgaaaa aggtagccaa tatacttcat tatataaact
     1441 tctagaagat gactatgatg tcaaagctac gagctctatt atggaaataa acggtttatc
     1501 tgcaacacct gagctttccg acttattgca atgtatagta ggagatccgc tatttatttt
     1561 agaaaaggtt ggcaaagata aaaataatca accatttcat tattctcaat ccacagttcg
     1621 ttgtgataga attagttatg ttattgaagt cacagaaaat atcactttag atacggtgca
     1681 taaaaatgat tcagatccta aagtaataga ttgatattaa aagaaatcat acccaaagca
     1741 atcaattttg attagatttg ggcatgattt ttatttttac taatattctc taaatacatc
     1801 aaaaaactta tttatattcc aaatttcacc tgtacttctt gtcgcagata tttaatatcg
     1861 taaataacat catctgctag acttacatct tgagaaaaac gtttatcttt aattgcaaga
     1921 accttcatat ttgctctttt accagctttt ataccattat aagaatcttc tatcaccaga
     1981 cattcttcaa ctggaattct gagctgttct gaagctttaa ggaatatagt aggattaggt
     2041 ttactttctg gcaaatctct tccgctaatt aaacaatcaa aatatgactc tattttacaa
     2101 gtggtaacaa cttcatttat agtatccatt ggactggaag aagcaattgc catttttatt
     2161 tttttctttt ttaaccattc aagctgttca attagtatat taggaaaaat atccaagtaa
     2221 tcaatctttt gcgatttcca atatttagta aattttccct tcaattctaa actgttacat
     2281 ccctcattgt acttttctat atcatcaaaa atacttgctg tagttccaat ctttttttga
     2341 taagaataca aaggatagct taaattttct tgttttaaaa aattaaatac ccacttttga
     2401 tacataatct cgctatcaat taaaactc
//
LOCUS       DACDIH010000152         2384 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.155, whole genome shotgun sequence.
ACCESSION   DACDIH010000152 DACDIH010000000
VERSION     DACDIH010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.155"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(386..727)
                     /locus_tag="IV281_002775"
     CDS             complement(386..727)
                     /locus_tag="IV281_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8781702.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(727..957)
                     /locus_tag="IV281_002776"
     CDS             complement(727..957)
                     /locus_tag="IV281_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8781703.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1244..1582
                     /locus_tag="IV281_002777"
     CDS             1244..1582
                     /locus_tag="IV281_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781704.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(1787..2026)
                     /locus_tag="IV281_002778"
     CDS             complement(1787..2026)
                     /locus_tag="IV281_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP8781705.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2002..2349)
                     /locus_tag="IV281_002779"
     CDS             complement(2002..2349)
                     /locus_tag="IV281_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP8781706.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT          737 a          448 c          365 g          834 t
ORIGIN      
        1 ttctttccta gacagatctt gatcccaaat tttacgtttt tgagcatcga tatagtttaa
       61 aatgacatta ctcatatcta aagagtttag attttttagg actaaaactg tcagttgttc
      121 tttttcttct ttctgttaaa gtattgtcgc gacttttcgt atacgttaag atatgtcgtg
      181 aaaaaacaag ttttgtgaca caaaaaaacg aatgacttga ggaaatcacc tcaatcattc
      241 gtttaaaatg ctatatattc tttagtttaa gatacggaaa ttaatgccat cgaccacact
      301 agtagaatga acgacattta cttggccttt ttttaatttc gttatgttta gcatataaaa
      361 atattataga gtttacttac aatgcttaaa acatatactg tataatctga tcaattttag
      421 ccatgtcttg caatggtaag ttttctattt ttttgagttt tcgttcctta aaatcaagag
      481 attttaattg tgtaataatt acttgtcctt ttatatctag ctcttcaggt aatgagtaac
      541 gtgtcggtaa attctttata gtggaagtaa ttggacatat gaccgaaaac attgttttac
      601 ggttaaattc ataacgagag acgactagtc ctggtcgtct tttttgaatt tcttttccta
      661 cggagggatc aaaatcaatc catacaatat cacctttttt aggaatataa attctttctt
      721 ctgacactat aaaatttctc tcccttcagg agttaaatct tcccattcgt ctttctcata
      781 atactcggcc tcttccccac cactaaatgg atcttcaatt tttggaacta acgtaattgt
      841 tccatcatca gaatacacaa cgatatattc ttggttttct gaaggtttct ttccgttatc
      901 agaaggtaaa gttaccacta cagaacttcc ttgtaatcta gattttgtaa caagcattat
      961 catcactccc ttaataaaag tatactatat aacactaagt aacgcaaaat tcccctttgt
     1021 aaactatcta gaaaattgtt ttgtttaaag tatatcttat tggatataaa tagaaataaa
     1081 ttgtagccag ttcatttttt ctttaactaa aaaaataatc caagaaacat tgaacctatg
     1141 gatccaaaag ctacccaaat actatagcta accccccgct accttcaatt ttgcagataa
     1201 aacgctttgg gagaaaattt caaagacaac aggccatcct atcatgcccg attataaaca
     1261 aaaagctggc tattcctttt gctctgacaa atttcaattg ggaaagttca cgaaactagc
     1321 aggtgaagaa gtttcaactg ttcggatcaa agaatgcccg cttcaaatag aaacattggt
     1381 tcaacgaatt tcaactagac aagactttgc tattgtagaa cgtgagatcg tagctatttt
     1441 tgtagaagaa gggattttac atgatccaac tcatatcaat gtggataaat ggcaacctct
     1501 tatctataaa ttcagagaat atacaactgc caaccagtcg ttaactttga attttagatt
     1561 ccaagaattc aaagagtcat aatgataaac atcgttaggt ataaataaaa aagactcgca
     1621 gcacgagtaa aaaacaaata gaagctatac attagaaagg tcgactgtga ccctatagat
     1681 gcagtatatg cagcttatga gcatttcttc tgttctcttt gtttttggga tctgtgctga
     1741 agtctttttc gttttttgcc gctctttctt tgaaaaatcg attatgttat accttctcca
     1801 ttactaattc aggcatctgc tgagtaatga tttttcgcgc aaaagaaata tctgttaccc
     1861 aaggttcgat ttgttcttca gtcaagacaa gcggcattcg gtcatggatt ggtgcgactg
     1921 tgcggttagg ttcagtagtc attatgattg attctgccat tttttcatcc tgatggctac
     1981 gataaaaacc gccaatatac actatttttt ccttatttga aaacataaat ttgtcttttt
     2041 cttcattcca ctcattggga aaacacaacg attttgcaaa aatggttgat gaaaggtctt
     2101 tttttcttct actgtctctg cacgggcatt aatcatcaat tgtccttttt taaatccggt
     2161 aaatccccac ttcatgattt ccggagtgag gatgttttct tcctcttttc ttaacgtcac
     2221 aacaagattt gacggaaaaa cttctccagc tgctaattct gctgcctgct tcggttgttt
     2281 cgtctgggct tctcgataat aattctgaag gagctctgac tctagatcaa aaaaataacg
     2341 accacacatc ctaatccctc ccaatctttt tttctatcat acac
//
LOCUS       DACDIH010000153         2380 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.156, whole genome shotgun sequence.
ACCESSION   DACDIH010000153 DACDIH010000000
VERSION     DACDIH010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.156"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(91..1506)
                     /locus_tag="IV281_002780"
     CDS             complement(91..1506)
                     /locus_tag="IV281_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017366819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8781707.1"
                     /translation="MSNKEEAKSIINFVGGKENIAALTHCVTRLRFALRDDTKVDKAG
                     LENLEDVKGTFNANGQFQVVIGPKVADIYEEVISQSGLSGANKEDVKKVAAELNLNWF
                     QRAVKILGDVFVPILPAIVTAGLLMGLNNLLTNPGIFFEQSLIEVYPNIADVADMINL
                     IANTSFQFLPALITWSTVKYFKGNPLLGIVLGLVFVHPDLMNAWNYGQAVTTGDIPHW
                     TIFGLSISKIGLQAQVLPALVSGYVLAKTELFLKKRVPETIQLLVVAPVAILFTSVIT
                     FIIIAPITIAIGNTIVAGIVWLFQKVPFIGGFAYGIGLAPLVVTGMHHAFLPVHIQLM
                     ANGGDYIWPIGVMSSLAQGSAAIAMNFLIQNKKLKGVGLAAGLSAYMGITEPAMFGVN
                     LKYKFPFVVSILTSGIIGGFMASQHVIATSVGISGIPGFLSIPFEFWPIFFICMLASC
                     VVSFGATYLYGKNKVLLQSFK"
     gene            complement(1499..1846)
                     /locus_tag="IV281_002781"
     CDS             complement(1499..1846)
                     /locus_tag="IV281_002781"
                     /inference="COORDINATES: protein motif:HMM:NF025668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781708.1"
                     /translation="MSPISIKEKGKNCLKVTTTVSNKGSRDGYEVVQLYIGAQYGRYI
                     RPTKDLVAYKKVFVKSGDSVTVSFELTNKDFKYYDEEFNEIIDDGAYWIMVGNDSSQA
                     LTNKEEIEVRINE"
     gene            complement(1859..2110)
                     /locus_tag="IV281_002782"
                     /pseudo
     CDS             complement(1859..2110)
                     /locus_tag="IV281_002782"
                     /inference="COORDINATES: protein motif:HMM:NF014026.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
BASE COUNT          783 a          498 c          372 g          727 t
ORIGIN      
        1 cactctcttc gggttttcgg tctgtactga aatcaaggta ttattgggaa tcccagctta
       61 aatcatagat accgtaaggg attttattct ttatttaaaa ctttgcaaca gaaccttatt
      121 tttcccgtaa aggtaagttg ctccaaatga gacgacacaa cttgctagca tacaaataaa
      181 gaatattggc cagaattcaa acggaataga caagaaacca ggaattccac tgataccaac
      241 agaagttgca atgacgtgtt gtgacgccat aaatcctcca attattccag aggtcagaat
      301 ggaaaccacg aatgggaatt tatactttag attaaccccg aacattgctg gctcagtgat
      361 acccatataa gcagacaaac cggcagccag tccaacacct ttcaactttt tattttggat
      421 taagaaattc attgcaatag cagcagaacc ttgtgccaaa cttgacatga ctccaatagg
      481 ccaaatataa tctccaccgt tcgccatcaa ttgaatatga actggtaaaa acgcatgatg
      541 catccctgtc acgactaatg gcgcaagacc aataccataa gcaaatccgc caataaatgg
      601 aactttttgg aataaccata caatacctgc gacaatcgta ttaccaatag caattgtaat
      661 cggcgctata ataataaatg ttataacact agtaaaaaga attgcaactg gtgcaacaac
      721 caacagttgg attgtttcag gcacacgctt tttcaagaat agctctgttt tagctaacac
      781 atagccggaa actagagctg gcaatacttg tgcttgtagt cctatcttag agatggacaa
      841 tccaaagatt gtccaatgtg gaatatctcc cgtggttact gcttgcccgt aattccaggc
      901 attcatcaaa tctgggtgta caaagactag acctaacaca atcccaagaa gaggatttcc
      961 tttaaagtat tttacagttg accatgttat taacgcaggc agaaattgaa aggaagtgtt
     1021 tgcgataaga tttatcatat cagcgacatc agcaatatta ggatatactt caatgagtga
     1081 ctgctcaaaa aagattcctg ggtttgttaa taaattattc agtcccatca gtaaaccagc
     1141 tgtaacgatc gctggtaaaa tcggcacaaa tacatcaccc aatattttaa ctgcacgttg
     1201 aaaccaattc aaattcaact cagccgctac ttttttaacg tcttctttgt ttgccccact
     1261 cagcccagat tggcttataa cctcttcata gatatctgct actttaggac cgataacaac
     1321 ttgaaactgt ccattagcat tgaaggtacc cttcacatct tccaggtttt ccaatcctgc
     1381 cttatcaact ttagtgtcat ctcgcaaggc aaacctcaga cgtgtcacac agtgagtcag
     1441 tgctgcaata ttctcttttc caccaacaaa gttaatgatt gatttagctt cttctttatt
     1501 actcattaat tcttacctct atttcttctt tattagttaa tgcttgagat gaatcattgc
     1561 ctaccatgat ccaataagca ccatcatcta taatttcgtt gaactcttca tcataatact
     1621 taaaatcttt gtttgtaagt tcaaaagaaa cagtcacact atcccctgac ttaacaaaaa
     1681 ccttcttgta ggctaccaag tcttttgttg gtcttatata acgaccatat tgagctccaa
     1741 tatatagttg aacaacctca taaccatctc tacttccttt atttgaaacg gtagtagtca
     1801 ccttcaaaca gttcttaccc ttctctttaa tgctaattgg tgacatagaa aaagtggtat
     1861 aactcaaccc atacccaaag ggaaattgtg gttcattgga tacatctaag tatttggtcg
     1921 tattaaactt ttcacttgaa tacggtctac cagtattgaa ataattataa taaataggga
     1981 tttgtcctaa atgtctcgga attgttattg taagctttcc ggctgggttt attttgccac
     2041 aaaggacgtc caaaatagca ttgccagcct ctgctcctag atgccaagcc tcaactaatc
     2101 ccttacatat agatttttaa attacacact agtttaaact aaaacaaaca aggaaaaact
     2161 taccatttaa tcaaaaaatt agctcatatg aatatattag aatgtaaatt agactggtca
     2221 aaatctggtc aaaaaagagg acgaatacag gtagtcctct tttttgttat tttgatattc
     2281 taaatcattt tttattggat tattcccttg ttcgatatag acccaaattg atctatcaat
     2341 aattatcaaa ctgagtcata tagagacaaa aatttcaaaa
//
LOCUS       DACDIH010000154         2371 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.157, whole genome shotgun sequence.
ACCESSION   DACDIH010000154 DACDIH010000000
VERSION     DACDIH010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.157"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            155..1456
                     /locus_tag="IV281_002783"
     CDS             155..1456
                     /locus_tag="IV281_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8781709.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(1560..1760)
                     /locus_tag="IV281_002784"
     CDS             complement(1560..1760)
                     /locus_tag="IV281_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8781710.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          769 a          454 c          408 g          740 t
ORIGIN      
        1 tttttcgtac aaaaaaaccg tcttttcttg gctctataat ttctaggact gatagaaaat
       61 cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc cgttagaatt
      121 gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc atatcaaact
      181 actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga agagcattaa
      241 gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc gctgtccttt
      301 gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa aatcgctcat
      361 tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac aacgctacca
      421 ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata gaaactgctt
      481 tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac aagcaatgac
      541 gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac tcgcttctct
      601 gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa tggatgaatt
      661 tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg gcgatactgg
      721 aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt attttatgca
      781 atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga atgtttctta
      841 tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc gttttcacat
      901 tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga aaagctttga
      961 tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt ctcggcttct
     1021 gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca attttggttg
     1081 ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg acgaattacg
     1141 aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag aggcggacac
     1201 tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc attctataaa
     1261 aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc cttattcaaa
     1321 tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg cttatggctt
     1381 tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga ttacctatga
     1441 atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat ctcactctat
     1501 cagtcctatt tgacaaagag cccttttctt tcgaaaagac ggttaaaagt caatttttat
     1561 taagctttaa caatgtttgt tgcttgaggg ccacgttgac catcttcaac atcgtaagac
     1621 actgcttggc cttcttctaa agttttgaag ccgtcacctt ggatagctga aaaatgtgca
     1681 aatacatcat ttccatcttc accagtgata aagccaaaac ctttgtctgc gttgaaccat
     1741 tttactgtac cgttattcat aaataaattc ctcctgatac gtatcaatgt acgttttttc
     1801 gttgcaataa atatttgata ggcaaaagga gttttatcta tgtagatata tcgctcaaat
     1861 tacgtttcaa aatagattac tcatttaata tatcatgctt tttctaataa cacaaggata
     1921 atgcaatgaa atataaaatt cattgtcttc tattgtcttc gaatcgaaga aaaagttgaa
     1981 taaccgcgat tgattcactt tttgttttct tgtttatttg cttttatctg caacttttga
     2041 ctttaaaatt tcttaactat gaaaaccttt caattggtgg tatacttata aatggaattt
     2101 cgttatcaac gcgttcgttg cctttcagca agtatttaaa gaaaggagaa tggctatgac
     2161 attctataac aaagatatac aatatcgagt aaccttggat acagatttga atctatttgt
     2221 cgtttttgac aaagaaaata ccaatcatgt agcgacaggt gttacgatcg aacaagcagt
     2281 gcaagaatta aaaaaatctg cataaggagt ggaaaccctc atatggacga ttctcgttct
     2341 tctctttaaa cgcatctact ggtagataac a
//
LOCUS       DACDIH010000155         2356 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.158, whole genome shotgun sequence.
ACCESSION   DACDIH010000155 DACDIH010000000
VERSION     DACDIH010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.158"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1..355)
                     /locus_tag="IV281_002785"
                     /pseudo
     CDS             complement(1..355)
                     /locus_tag="IV281_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            431..586
                     /locus_tag="IV281_002786"
                     /pseudo
     CDS             431..586
                     /locus_tag="IV281_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            605..1882
                     /locus_tag="IV281_002787"
     CDS             605..1882
                     /locus_tag="IV281_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8781711.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1892..>2356
                     /locus_tag="IV281_002788"
     CDS             1892..>2356
                     /locus_tag="IV281_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8781712.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADE"
BASE COUNT          903 a          252 c          407 g          794 t
ORIGIN      
        1 tgacgagaaa tatttagaat tttgcacatc gctgatatgg aatatttatg cttgttggca
       61 tcaattactt gtcttttcgt ccgaatatca gcgccgcttg ctttaaaata tcattctcca
      121 tttcgagttg cttatttttc tttcttagtg cgatcaattc tgcttgttct ggtgttaagt
      181 tgtctctttc tttgaatgag cccgttgatt gtgcttgctt catccattta tcgaaagaag
      241 aaggcgtaag ctcatactct cgaataattt ctgcgcgagg cttaccagcg agatagagat
      301 cgacgatttg ttgtttgaat tcttttgaat aggttcttcg ttgacgtctt ggcatgaaaa
      361 atcctccagt gtgtttttta ttattctaca caccttattt tttctgtcta gtctagtgta
      421 gccgattcag gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat
      481 gaataaagat tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa
      541 aaatgcttgt ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag
      601 gaaagtgata aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat
      661 ttttatcata ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc
      721 aagcaatgaa atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa
      781 aaaagatggt aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat
      841 tgaagaaaaa ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag
      901 cggagaacaa agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact
      961 cgaagctaca aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa
     1021 agaaggtaaa aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc
     1081 taaacaaaac aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa
     1141 aagtggaaaa ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca
     1201 ggaaacatat acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc
     1261 aacaagccaa gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat
     1321 gtattctggt agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat
     1381 tgatgagtca aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc
     1441 agtgagtgga ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat
     1501 ggttgttctt tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat
     1561 attcttatct attggaacat ctaagataca aattataatg caatttatat tcgagttaat
     1621 attcatatcg ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt
     1681 aattgtagat ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa
     1741 taatagtagt tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag
     1801 tattattgtt ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga
     1861 aatcttatca aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc
     1921 atatagttat gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact
     1981 tgggaagttt tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt
     2041 acttgctgga cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca
     2101 aaataaagga tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa
     2161 tttaatagat tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga
     2221 aagtatcttg tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt
     2281 atctggtgga cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat
     2341 aatactagct gatgag
//
LOCUS       DACDIH010000156         2352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.159, whole genome shotgun sequence.
ACCESSION   DACDIH010000156 DACDIH010000000
VERSION     DACDIH010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.159"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..544
                     /locus_tag="IV281_002789"
     CDS             <1..544
                     /locus_tag="IV281_002789"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8781713.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1017..1589)
                     /locus_tag="IV281_002790"
     CDS             complement(1017..1589)
                     /locus_tag="IV281_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8781714.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1605..2210)
                     /locus_tag="IV281_002791"
     CDS             complement(1605..2210)
                     /locus_tag="IV281_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP8781715.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          740 a          469 c          359 g          784 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaat gtgtccaaga tattgactca aatccagtaa tatcacaata
      601 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
      661 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
      721 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
      781 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
      841 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
      901 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
      961 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1021 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1081 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1141 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1201 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1261 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     1321 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     1381 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     1441 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     1501 ttctctacgc cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     1561 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     1621 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     1681 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     1741 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     1801 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     1861 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     1921 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     1981 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2041 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2101 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2161 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2221 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     2281 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     2341 gcacagtcta gt
//
LOCUS       DACDIH010000157         2276 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.160, whole genome shotgun sequence.
ACCESSION   DACDIH010000157 DACDIH010000000
VERSION     DACDIH010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.160"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            47..247
                     /locus_tag="IV281_002792"
     CDS             47..247
                     /locus_tag="IV281_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8781716.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            1001..2227
                     /locus_tag="IV281_002793"
     CDS             1001..2227
                     /locus_tag="IV281_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP8781717.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT          812 a          327 c          479 g          658 t
ORIGIN      
        1 ttcttgcaag gttacttgca agaacttttt tggaggatat acatttatgg aacacggaac
       61 ggttaaatgg ttcaacaatg aaaaaggatt tgggtttatt acagtagacg gcggagatga
      121 cgtatttgtt catttctcag caattcaagg agacggcttc aagagcctag aagaaggtca
      181 agaagtagaa ttcacgattg ttgaaggtgc acgcggacct caagctgctg aagtgactaa
      241 attataataa tttgttgaat aaagacacga cttcttagag agtcgtgtct ttttgtctat
      301 ttatgaagtt tttagatttt ttggctaagt aaatgcattt gttcacttga catgtcctaa
      361 ctaattatca agcaaggaac attttcttcg ttagtaatgg attttaaaga aatttcagtt
      421 tttggatgtt gaataaaaga ccatttgtga aaccactagt atacatactc attaccatgt
      481 caattctatt actttaaaag aaaatcgttt tactttgaag aggagcatat ctatccatta
      541 aaagatgatg gtaatgcaat cactagttcg agtcatttta agagccatgc agtgagctgg
      601 aaataaaacg aatagaagat gggtttaaca aaaggaccag tcataggaag aaattgtcgt
      661 gaaatcgaat gataatctga acggtaggca aagactgtta aaaagatggg aaaggtatgt
      721 gcgatcattt tactgcacga ttatttggaa aataccttgt tgttcgcttt ttctggaaaa
      781 aatacataaa aaaagataaa acgattgcga actgcgtgta ttcggtcttt cgatcttttt
      841 cacggtatgg tatgatagtt aagaattttg agaaatttta aaaaataata atcgttacac
      901 aaaaatgttt tttaaagttt tcttatgatt aaaaagtgaa tttgctgatt gaacagttat
      961 atgagacaat attttagaaa atgtgaaaca gaggtgtatt atgaagcgat ggcaaaaagt
     1021 tctattaacc ttgttagggg tattcgtttt gctgattgcc ggattatcag catacggaat
     1081 cagatttatg ggagaagcga gccaaacagt aaacaaaatt tctaaaaact ctaatcgaat
     1141 atcctccaaa cgttctgaga gagtaagcat tgatgacaaa gaaccttttt cggttttgct
     1201 attaggttta gataccggtg gattaggaag aacggaacaa ggacgttctg atacgatgat
     1261 ggtagtaaca gttaatccga aacaaaaaaa atcaacgatc atcagtttgg accgagatat
     1321 ttatacgaat atcgtaggtt atggaacggt agataaatta aatcatgcct acgcatttgg
     1381 tggtgtcgaa atggccatgg attcaattga gcaactgctc gatattccta ttgatcatta
     1441 tgtaacgatc aatttggatg gcatggaaga tttgatcaat gcggtcggtg gcatcaaagt
     1501 gaataataaa attgacttta ctttggatgg tgtccatgta ccagtaggtg aacaaacatt
     1561 gaatggagaa aaaggtttag cctattctcg aatgcgacac gaagatccag aaggcgatat
     1621 cggtcgacaa cgtcgtcagc gggaagtagt gacgaaaatc gtcaataaag ttttaagttt
     1681 agatggcgta agcaattata gaaaaatttt aaaagcagtt gagaacaatg tgacgacgga
     1741 cttagattgg gatgacatgc ttgatgtagc gacgaactac accccggcgt ttgaaacaat
     1801 caaacaagac cagttgcaag gcgaaggaca aatgatcaat gatatctact atcaaatttt
     1861 aggaaaaaat gatcttcttt cgatccaaaa tgaactgaag aaacaattaa atataaaaac
     1921 aagcgataca ttacctaatt tgaaaaatga gaatgcttca atgatgttct ttgatgattc
     1981 ggaagatggc gattcgacaa acggtgatgg atcgaatagt gaagggtaca ccgattattc
     2041 ttcctattca gaaaacacat atgatcagta ccaagatccg aatacctatt atcagcaaac
     2101 agaagataca agtcagtact atacaccaag tcaagatact caaagttatg atccttacgc
     2161 tggacaggaa caaaatggtt atggttatga ccagaataat gagtatggaa caacaagtgg
     2221 gtactagaaa aaaatagttt ggttaataaa gagtgccacc taaattagac aaaaaa
//
LOCUS       DACDIH010000158         2271 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.161, whole genome shotgun sequence.
ACCESSION   DACDIH010000158 DACDIH010000000
VERSION     DACDIH010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.161"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            197..2059
                     /locus_tag="IV281_002794"
     CDS             197..2059
                     /locus_tag="IV281_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8781718.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2059..>2271
                     /locus_tag="IV281_002795"
     CDS             2059..>2271
                     /locus_tag="IV281_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HAP8781719.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLF"
BASE COUNT          810 a          340 c          446 g          675 t
ORIGIN      
        1 aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt ttaacagtac
       61 atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct ataagttgca
      121 cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta tattagcaat
      181 gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt tgatttactt
      241 ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa atgttagtgt
      301 tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc ggaagcaagt
      361 cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg atcgaatcaa
      421 attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc aaagtctccg
      481 aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg gaatgaaaat
      541 ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg tgctggaaaa
      601 taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt tttggatcat
      661 aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag agttcacgga
      721 ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg aatactttga
      781 aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa tcattcgaat
      841 gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat tactaaatat
      901 tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga ttggtcgtta
      961 tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg tcatgcagca
     1021 ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata tctatagtga
     1081 tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg tgatttataa
     1141 agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga cgaatctgta
     1201 tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat ataatccttt
     1261 taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc aagcagttcg
     1321 acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat atattgcctt
     1381 acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag ccgtctacat
     1441 ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg aagaattaaa
     1501 taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga ttgctaccaa
     1561 agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc ctttcatcaa
     1621 agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa taaaaaaaat
     1681 acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa cgtccatcaa
     1741 taaagatact ttagaagact ttagcaagga attgattttg aaagcttata cgatttatga
     1801 acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag cttttttgct
     1861 tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg cagaaggtcg
     1921 tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg aagcagtttt
     1981 tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct catatataaa
     2041 agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat cgaggaaagt
     2101 caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt agtagaacat
     2161 gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa tgagatgctg
     2221 aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt c
//
LOCUS       DACDIH010000159         2260 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.162, whole genome shotgun sequence.
ACCESSION   DACDIH010000159 DACDIH010000000
VERSION     DACDIH010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.162"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..866
                     /locus_tag="IV281_002796"
     CDS             <1..866
                     /locus_tag="IV281_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8781720.1"
                     /translation="RSRQTIHKVYLFFKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQ
                     SEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQ
                     ERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIITLK
                     PCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPGTP
                     SQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNNIPRKSLDYRTPLEVFLS
                     YVSIDDLSNLI"
     gene            1343..1543
                     /locus_tag="IV281_002797"
                     /pseudo
     CDS             1343..1543
                     /locus_tag="IV281_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          779 a          370 c          429 g          682 t
ORIGIN      
        1 atcgttcaag acaaacgatc cataaagtat atctgttctt caagcaagga aaatcagcct
       61 tagaatatta tcaacagtat aagaaaaaca aatcaaactg tggtagacgt ccgcttgttt
      121 tacccgagga acaatcagaa tatattcaaa gaaaggttgt tcaaggatgg acacccgatg
      181 tgattgttgg tcgtgcagcg tttcctatta gttgttctgc tcgtaccatt tatcgtatgt
      241 tcaaaaaggg gctatttgat tcttctgact taccgatgaa aggcaagcgt aaaccgaatg
      301 gacatcaaga aagacgtgga aaacaaactt tccgccgctc tattcatgaa cgtgaaaagg
      361 attatagcca attctcaaat gagtttggtc accttgaagg tgacactatc gtaggtctga
      421 aacataaaag tgctgtaatt accttagttg aacgattatc aaaagttatc atcacattga
      481 aaccgtgtgg tagacaagcg attgatattg aaaaaaaatt aaatcaatgg tttgaatctg
      541 taccgaaaaa cctattcaaa tccatcactt ttgattgtgg aaaggaattt tcaaattgga
      601 aacagatcag taatgtcaat gatattgcca tttatttcgc tgatccagga acgccgtctc
      661 aaagaggcct aaacgagaat tctaacggat tgttacgtag agatggttta ttgaaatcta
      721 tggatttcaa ttcagtagat gaatttttta ttcaatctgt cgcatctaaa cgaaataata
      781 ttcctagaaa atcactggat tatcgaacac ctttggaagt atttttgagt tacgtaagta
      841 ttgatgatct gtctaactta atttgacaat taagacattt aaaaagaaaa gtgacagata
      901 ggaataaaaa cttattaaat gtagttatga tctgttatat ttcatttcta atactaggtc
      961 ttccacaagc agtgtttcct gaaatgtttt ctggagactg gggtattgtc agctattcta
     1021 tattaacatt gattgctatg gttttaggta cttcatttta taatgctttt gtaagtaagc
     1081 atgttgagga atatgaagta ttaatgtact taaaagagaa aaaaacaagt aattaatatg
     1141 agaacaccac gcatagtttt tattttgaga tattctagaa tgaaacacat taagaacgaa
     1201 agcaaagcaa aagacgccag aacttctact accttctaag ttttttcata tttgtttgtt
     1261 atgctataca tagatctgca taattagact tgcgctgccc gttattgaag tttcaggcag
     1321 cgctcttttt gggggatcga taatggaaga agaaaaaata tttgagaaac gctggcagct
     1381 agcatcaagt gaacaaagag caagatacaa taatttgatc acctcctacc ccactgttga
     1441 ctagacatat aaggaaaaga aatacttact ttggctgtgc cagttagata tagatacctt
     1501 tgaaacattt gaagtgatcc tagataaaat taaacagaat tgatcagaaa caagcgaagc
     1561 cttctctcgg ttcgcttgtt ttgctattta ttagttggct cagggcaact ctaagctcgt
     1621 ttgaaagaaa accgatggat aatccccttg tttgtaccac tttagaatac atggatgtac
     1681 gtattgccat tgactcctat tagttactga aaaaaaattt aaacaagttg agtattgcag
     1741 ataaatagct ccagtgaggt gaataactgc tccactcaag gcaatagata gctccatcca
     1801 gggcaacaaa ctgctccact ttcatattta atagatgaat aaccttctgt ataatagaaa
     1861 ggcatacctt tcggtatgac aacttataca gaatgttatt ttattggttc actatcgaaa
     1921 aattttggag ctttattttt ccggtatttc tcaacgaacg attagtttaa ctgtgggaag
     1981 ctccagacat acgattcgag aagttacttc tcgagcagac aggaaggggg tgactgaact
     2041 aaccgctgat atgagcgata cttggctgta ggaatatctt tttccggaaa aacaaacaac
     2101 agcacgaggt taccttcctg tagactggga atatgttcat aaagaactaa tgaaaaagaa
     2161 tatgacattg aaactccttc atagagaata tggggtacat ggaacataga aacgaaaatg
     2221 cacggtagaa aagcgaatga atatagtata aattctacaa
//
LOCUS       DACDIH010000160         2092 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.163, whole genome shotgun sequence.
ACCESSION   DACDIH010000160 DACDIH010000000
VERSION     DACDIH010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.163"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..302)
                     /locus_tag="IV281_002798"
     CDS             complement(<1..302)
                     /locus_tag="IV281_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781721.1"
                     /translation="MNKLPEKNRVFRLKGDLSQFKIDENGQFFVWMRHEHCWCNPFVL
                     KKENEIRTKIANGEWDYLFRWEDESFIANDMDHAFVSFLDNTWGTWYDCYLEKLGF"
     gene            complement(299..526)
                     /locus_tag="IV281_002799"
     CDS             complement(299..526)
                     /locus_tag="IV281_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781722.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
     gene            complement(693..875)
                     /locus_tag="IV281_002800"
     CDS             complement(693..875)
                     /locus_tag="IV281_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781723.1"
                     /translation="MTSGELMKYEKKVAPARKGEDDQKLSVDLRQYLTTGRSNKFQIR
                     RPVDLTNNFVIGEFIL"
BASE COUNT          648 a          424 c          255 g          765 t
ORIGIN      
        1 tgaaagccca atttttctag gtaacaatcg taccaagtac cccatgtatt atccagaaat
       61 gagacaaatg catgatccat atcatttgct ataaaagatt catcttccca tctgaagagg
      121 taatcccact caccatttgc aattttagtt ctgatttcat tttctttttt taacacaaaa
      181 ggattacacc aacaatgctc atgtcgcatc caaacaaaaa attgtccatt ctcatctatt
      241 ttaaattgac tgagatcacc tttcaatcta aatacacggt tcttttctgg taacttattc
      301 atgcttcaac cacctcatac tgttctctca tacctttgac ttcattaaaa aaaactctcg
      361 ctttttcttc atcagtaaag tttttataca actgattaca ataatgaggt ttttgaagtt
      421 tctgaatacc tatactaact tcaaatcctt cttcatgttg aattaggatt acttctgtgt
      481 ctctacctag aaatattcga tctaaaattt ttatgtttgg tttcattatt taacctccac
      541 tataattatt tcactcaata aggtttcact ttatgaaatt tctatagttc tcttttcaca
      601 aaacgtacca tgttctctaa gactgttttt agtctcaaat atacatgttt tttcttatcc
      661 tttccttata cactcaaaat caaagataaa acttaaagaa taaactctcc aataacaaaa
      721 ttattagtca gatccactgg tctcctgatc tgaaatttat tacttcttcc tgttgtcaaa
      781 tactgtctca aatcaacaga aagtttttga tcatcttctc cttttctagc aggagcaact
      841 tttttttcat acttcattaa ctctccactg gtcatctttc caatgatagt gtaattaaaa
      901 tcactttcaa aactttggta aatttttcca tctgtattga atatatcagc tgtttcaata
      961 tcttctttgt attcattgtc ttctcctgaa ccgattgtat caaatagaaa ggcaagacga
     1021 attccatctt cttcaagcat tgttaatctt tttttaatta gcccaacggt ttcaatagat
     1081 aacttatcga agccgtgaat cataacaata tcatcttttt ttgcttgtga tgtgatgaaa
     1141 tcaaaggtat ttaaaaattg agcttccata acattggttt ctgctcttag atcacctaag
     1201 ttatagtaaa actgtaattt gtcactttta ccgatttctt tcagtgtcgt tctttgatta
     1261 aacatcctag agaagctgtc taatgctgtt ctcaatgtgt cttttaatac aataatacga
     1321 tcgacttctt tttcagtacc agattttttg gcttttgttt cagcattagt caaatagcta
     1381 ataaaatccc caagaactgg cattgtatca ctactgttca ccaaaagttt tgaacgttct
     1441 tcataattaa tatcccaaag tttctgactt atataaaagt tacctaattc ttttttcaat
     1501 aaaactctag ttgcagaatc taatgttctg cctgctaaca aataaaaaat ctgagttagt
     1561 ttatcgagat tccgtctata taaatcacca atgtcctttt gattaccaaa aagctccaaa
     1621 gagttaagac cttcttcagc caaattaacc ttttctatta cactgtttaa cgaaggtttg
     1681 gcaacaaatg ttctaccagt tggatcacca aagtaatcaa atttatttaa gacaatatga
     1741 aaagttcgtt tatgatttgc cattgcatta ttagcaatca attgccccca aagtgatgat
     1801 gctgagattt tttcatcata gcctttaatt gtagcgtttt tgtgtgatgc cactaaaact
     1861 ttacttgtaa aagaatcatc cagtgccata aaagctacac cacttgcgta gctagtagac
     1921 atagtgccga tagcccatcc attcgcatca tctaaacctt tgcgaattgc atgatcattt
     1981 cctgcaaaat ccgaactcat agacgtatac tcatagacat tcccgattgg ttcacttaaa
     2041 agtctatcaa acagatcacg ttgatctcct ccatatgaca taagctttaa tc
//
LOCUS       DACDIH010000161         2016 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.164, whole genome shotgun sequence.
ACCESSION   DACDIH010000161 DACDIH010000000
VERSION     DACDIH010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.164"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1959
                     /locus_tag="IV281_002801"
     CDS             <1..1959
                     /locus_tag="IV281_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8781724.1"
                     /translation="LYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLNGK
                     YPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHEKP
                     DLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDASTD
                     PAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPFAL
                     YEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYRTS
                     IVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEAKS
                     YIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYEDLK
                     TCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYSRI
                     NNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQHAG
                     VLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDEIL
                     EVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQDK
                     WPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT          682 a          342 c          425 g          567 t
ORIGIN      
        1 ttatataatc ttgatgttaa gacccaaagc ggttttaagg tacgtttatc agggaaaatg
       61 agaggaaaag caatcttaga cttccaaaca gcaagacatc aaaatggcat agctgtgaaa
      121 ttaaacggta aatatcctta tgatgacggc attgaatctt catgggaaag aaaaaaaaga
      181 ttactgaaaa aagggataaa ttatgcaagg actcatggcg taaaaaaagc aatccgccgg
      241 gtcaaactgg aattgaaacc aggatcgatc gactatgcag agtggatcag tcgtcatgag
      301 aaacctgatc tgaaagagca aaggaaagaa gtacaaggat ttgcctaccg cccattgatt
      361 tccatactta tgcctgtcta taacgttgaa atcaaatggc tcgaaaaatg cattgattcg
      421 gtacttgatc aaacgtatga tcactgggaa ctatgtatca gtgatgatgc ttctactgat
      481 ccagctatcc gaaagtgtct agaatcctat caagcaaaag atgatagaat taaagtcgtt
      541 ttccgacaag aaaatggaca catcagtctg gcaactaatt ctgcattgga aatggcagaa
      601 ggagagttta tagcgctctt agataatgat gacgaattac ctccatttgc cttatatgaa
      661 gtggcaaaag tgctaaacgt acatccagaa ctggatttga tctacagtga cgaagataaa
      721 atcgatgcag atgggaatcg ctttgacccg cacttcaaag cagactggtc tcccgataca
      781 ttgatgggaa ataattatat ctctcactta ggcgtatatc ggacaagcat tgttaaggaa
      841 cttggcggtt tccgtaaagg atatgaaggc tctcaagatt atgatttggt tcttcgtgtg
      901 acagaacaaa ttcctgcaga acacatttac cacattgacc gtgtcttgta tcattggcgg
      961 acgatcccgg gatcaactgc aagcaatgga gaagctaaaa gctacatcta tgattctgga
     1021 gtgaaagcat tgacagatgc tctatctaga agaaatatca agggaagcgt acatcctggc
     1081 agaatttctg gtttttatga aatcacttat gatgttttgc aagaagactt ggtcagtgtg
     1141 atcattccaa ctaagaatgg ctatgaagat ctaaaaactt gtgtggattc gatcatcgaa
     1201 aaaacgtctt atccaaatta tgaaatcatt attgcagata atggaagtac agatcctaaa
     1261 atgcaagaat tatttgcaga atacaagcat cagctgaaag accgatttat cgtcgaatta
     1321 attgacattc cattcaatta ttcacgtatc aacaatctgg cagcagaaaa agcaaacggc
     1381 aaatactttt tattcttgaa taacgataca gaagtgattg aacctgactg gatgacagcg
     1441 atggtttctt atgcacagtt tgatcgaatc ggctgtgtag gagcaaaatt attttatccg
     1501 gatgatacaa cgcagcatgc aggtgtatta ttagggatcg gtggcgttgc aggacatgca
     1561 ttgaataact atgatcgaac acattgtggt tactttggcc gattggtcat tgacgtcaat
     1621 tacttagcag tcactgcagc ttgtatgatg gtcaaggcag ctgacttcaa tgctgtcaat
     1681 ggatttgatg aaatattaga agtagcattc aacgatgttg acttgtgctt gaaagtttat
     1741 gaactgggtc gttacaatgt ctatgcacat caagcagaat tgtaccattt cgaatctaaa
     1801 tcaagaggtt atgaagatac accagaaaaa caaagacgtt ttgctgggga aatcaaaaaa
     1861 atgcaagata agtggccttc ttatattgct catgacccat tttataacga taatctaact
     1921 aaagaaggaa tcggcgattt ttcgttgaga cctgattgat taaaagaact gtattttcat
     1981 ttttattaat gaaaatacag ttcttttaat ttaatt
//
LOCUS       DACDIH010000162         2000 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDIH010000162 DACDIH010000000
VERSION     DACDIH010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.165"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            63..1556
                     /locus_tag="IV281_002802"
     CDS             63..1556
                     /locus_tag="IV281_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP8781725.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            1691..1987
                     /locus_tag="IV281_002803"
     CDS             1691..1987
                     /locus_tag="IV281_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP8781726.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
BASE COUNT          665 a          311 c          425 g          599 t
ORIGIN      
        1 ctatggctga gattaaagct aaactgtgtt ctctttttgt ttgagaaagg acgttacaat
       61 cgatgttgag cgaaatttat tcagctattc ttaaaaacgg cttacgtaca tacaaatatc
      121 gaaacagcac catgaaacct gcaggtcata ttgattccag taggagtggt gctatctttg
      181 cgtaccgtag caagcaatta atgaatgttg ggcgtggtat ggtcattact tcggaagaag
      241 ctattttaga gaacgaaaag aaactaacgc attggacacc taatacctat cgctttggta
      301 cttatgcgga tgattatcga acagtggtta aaggtcattc tgagaaaaac ttaagtcaaa
      361 tcaacacatt tgtagttgat atcgattcaa aagagaacca tcaaggcgaa attattttag
      421 cctgtttaga tcaagtgggc tacatgccaa cgctaatttt agaatcagat catggttatc
      481 aagtttattt tgtactaaag acaccaggct atgtcacaaa gaaaagcaat ttcaaggtga
      541 tcgatgtagc aaaaaaaatt tcaaaaacaa ttcgtctaca gttggctgaa aaattgtccg
      601 gagtggatct aggatgtaac cacttcggaa ttgctcgatt cccacaagaa aataatattg
      661 tttttaaaga aatgaactat cagtattctt ttgctgactg gattcaatgg tcgatgaagc
      721 ttgaatcaga tcaaaaaaca aaagaagatc gagaacgaaa actaattgtc tttcctgaga
      781 aaaaagaaat tcgtcaagta gatgaaccgt ggttcgatat gttacttcat aaagcaaaca
      841 tcgtcggcgg agaaggacgt cttggtcgaa ataatgtgat tttcacgctt tctttagctt
      901 attactcttc aggttatgca ttggatactt gcgaatacaa tatgttcaca ttcaacgaac
      961 ggttacagga accgctatct gaagcagaat tagtaaagat tgtacgtagt gcttattctg
     1021 gtaattatca agcagcaagc agagaattca ttattgaatt gtgccagcaa tgggtttcac
     1081 caaccttaaa aaataatgat ctgttcattc aacgacgtgg ctggtggaaa tttaaaaagc
     1141 cacgagatca aagaaaatat agtcataaaa atgaatggaa agaagatcta ttggcttatc
     1201 taagcgaaaa aagtcttgct agtgagcctt atttgaacct gtcaaaaaaa gaattggctt
     1261 cacaactaac gatgcccaag cgaacacttg ataagatctt aaatgagtta cgagaggaga
     1321 ataaagtctt ctacaaggta acacgaggaa gaaatggcgg aatgatcatt gcttcagtca
     1381 aaacgttgtt cgcgagggtt attcaactaa gtaaagaaaa gagactagat tattcaacaa
     1441 ggatcacaga ggtatttgga ctcaacgaga gcttagtaga agagacattt agacaattat
     1501 caaccccctt gaaccctctt aaagaagtga atctttttga agtagataca ggttgaaaaa
     1561 taaaaggtgc aaattgcctt atacaatata tctatgatac ttctgtaggt gttgctccct
     1621 ttgcctagct acgtgtaggt ggtatactta taactgcgta tgcagtctta tgtatcatgg
     1681 aggaagttgt atgtcgttaa aaaattatgt ctattcacat cctgttaatg tgtttatcat
     1741 tggtcgtttg ggattatctg tggaagtgtt ctgtgagctt tatggtttta agcaggggac
     1801 tttgtcttct tgggttacca gggaaaaaac agttgcttct ttgccgattg agtttcttca
     1861 tgggttgtcc ttagcttctg gggagactat ggatcaggta tatgattgtt tgtgtgtgtt
     1921 agagcaggag tatattgagt ataaaatagc taacgagtta agaaaaagga agaagtatat
     1981 ccagtagtag tttttattta
//
LOCUS       DACDIH010000163         2000 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.166, whole genome shotgun sequence.
ACCESSION   DACDIH010000163 DACDIH010000000
VERSION     DACDIH010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.166"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..430)
                     /locus_tag="IV281_002804"
     CDS             complement(<1..430)
                     /locus_tag="IV281_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8781727.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
     gene            774..1181
                     /locus_tag="IV281_002805"
     CDS             774..1181
                     /locus_tag="IV281_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781728.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            1195..1596
                     /locus_tag="IV281_002806"
     CDS             1195..1596
                     /locus_tag="IV281_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781729.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1598..1969
                     /locus_tag="IV281_002807"
     CDS             1598..1969
                     /locus_tag="IV281_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781730.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          712 a          281 c          328 g          679 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tctaactttt ggggtgcaca
      481 tcatttttga ggggaagtac atatagttgt tgatatagtt gctaatattg ttagctaatt
      541 tgttagtaaa actgtatacg aatttagata gtaaaaaaac agtttgttcc ttatatagag
      601 gcacttttac tactgattaa atgtcagata tgcttacaaa tatacttata aatatgctta
      661 caaaattagc taatatttcc gttgacaaag ctttgttact attgttaaac taaattgact
      721 agcaaaagtg aaacataaat ttattaaata aatgtaaaaa ttaggagaaa tcaatgtctt
      781 atagagtaca attaataatt tcagaagatg tagaaagtca gcaatttggc actaatgtta
      841 ttaataaagt aattaatcca gctctttcta ttaatgcgcc tttaattcct actgctttat
      901 cattttctgt aacagctgtg gtgtctgaga tagaggatac agaaaagata aaaatagtag
      961 aaattgaagt tttaaacaag aatgaaaaac aaattttttc tacgggtgaa gtatcggtaa
     1021 gcttgcctcc tcaagttaac gatataaact ttaatataaa cgccagaaat gtcttggttg
     1081 aagaagcagg agagcattat gctgttgtta aatttaatgg aactgagatt ggaaggcaaa
     1141 tctttgatat caaggtcaac aaaccagtgg aaaaaaatta aggagacgat acggatgctt
     1201 gatatagtac catcaaatac acatcggagt ggtagcttaa taaattttgc tgcgattttt
     1261 gcttgcgcat cggtaattct agcatctcca gttgttgtgg atgcaagttc tactccgaaa
     1321 actaaaaatg ataatcaact tgtaattact acaaaaaatg agattaacac aatttcaagt
     1381 aatgatggca aaatttttga tgtgataaat acggttttaa aaagaaaagc tgaaatcaat
     1441 ataaaatatg atgaagattt aaacttgtat ttctttgtta tcaaaacaac atctgaattg
     1501 ttttcctcag attacgatgt tttggatact ctcgataatg tattatctga ctataaatat
     1561 atggggaaat ctgtagtagc aactttgggg gaataaaatg tttgacttta atgaatattt
     1621 atctatttcc aaacaaattg aatcagatga taaatataat tctaaagaat cttatagaag
     1681 aactgctgtt tcaagagcat actacagtgc ttttaaaaaa tctgatgagt atttgaaaga
     1741 aaattatgat attatttata atggaagtag cggaaaagga agccaccaaa ccgtttggaa
     1801 tttgttttca acagttaagg aattgaatac cttaggaata cataatagtg ggtatagaat
     1861 gttagagaaa agaaaatgtg ctgactatat tcctaatgaa accattacga aaacagatat
     1921 ggcgctgatg aatcgagaag cagaaaaaat aataaacaaa ctcacctaat gaatttcaag
     1981 cgagtttgtt tgttattttt
//
LOCUS       DACDIH010000164         1980 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.167, whole genome shotgun sequence.
ACCESSION   DACDIH010000164 DACDIH010000000
VERSION     DACDIH010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.167"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            271..837
                     /gene="amaP"
                     /locus_tag="IV281_002808"
     CDS             271..837
                     /gene="amaP"
                     /locus_tag="IV281_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8781731.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            850..1038
                     /locus_tag="IV281_002809"
     CDS             850..1038
                     /locus_tag="IV281_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8781732.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1051..1608
                     /locus_tag="IV281_002810"
     CDS             1051..1608
                     /locus_tag="IV281_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8781733.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1640..1882
                     /locus_tag="IV281_002811"
     CDS             1640..1882
                     /locus_tag="IV281_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8781734.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          721 a          305 c          406 g          548 t
ORIGIN      
        1 taaatagaat cttgtaataa taactgagca taatctttgc aatttttaat cttgaataat
       61 atcctttaag tatagccagc aatcaaaagt aaatagagaa taagtaagca attgttattt
      121 cagtatcaga ctggcaataa aacgccgtta tgatccagca atgtttggta gcgctttatt
      181 tactgatgct atgctaactg ttcgctatct atactgtgat tgtcggtaga gttatttaca
      241 gggtaaaaat aagtttatag gaggtggaaa atgaataagg ggttaaaaat aatcggtgtt
      301 atcatgagtt tactgctact ttcagtattt ctagcgattg cgttgatcaa tgcgccgtat
      361 attatgcctg aacaattgga acgatttcgc ttctttactt taacgaatta ctacatgcaa
      421 caatatattt tctgggcagc tgtagtattt gccgttcttg ctattatcat cttgctagtc
      481 gttcttttct atccaaagtc aagaggaact ttcgttatga aacgggaaga tggcaaacta
      541 acaatcgaca aaaaagcgat cgaagggttg gtacgttccc atttacacga agaagagttc
      601 attcattcac ctaaagtacg gatacgttca acgaaaaatc gaatccatat ccatgtcaac
      661 ggagacttga aacgaacttc ttcattagta gggaaaacag gtgtattgat gcaagatatc
      721 gaagaagaag taactaaagt actcggaaca aaagaaacgg tcaaagtggc agtcacatac
      781 agcggttatg aagaacaaga agatacaaga gactacaagc actctcgagt ggaataggag
      841 ggccaaataa tgaaagaact attttcagca tacaagctcc ctattatctt tgggggtctt
      901 ggactactgc tagctgtttt atggttaacg attggatttt tcaagacatt gttgatcata
      961 atcatgacag caattggcgc aggaatcggt ttttatttga aacagtcagg aatagttgat
     1021 caatttttta aatcatagga ggaaacaaag atggaaaaca aaaacatgaa tacaacaaat
     1081 ggatcagttt tggcaaatga aaaaggagaa aaccgcatga tgaatggtga attgacttat
     1141 gaagacaaag tcatccaaaa aatcatcggt tatgcattga aagatgtaga tggtctattg
     1201 acattagatg gcggattttt ctcaaatatg gctgaaaaat tagtcaacac agataacgta
     1261 acagcaggta tcaatacaga agtaggtaag aaacaagtag ctgtggatct agatgtgatc
     1321 gtagaatacg gtaaagacat cgaagatatt tacaaccaaa tcaaagaatt gatcagtact
     1381 gaagtaaaca atatgacaca tttagatgtt atcgaagtga atgtgaatgt tgcagacatc
     1441 aaaacacaag aagaatacca aaaagatcaa gaaacagtac aagacaaagt aacagaagca
     1501 gcaaaatcca caggacaatt tgcttcacgc caaacagata aagcaaaatc tgctgtaggc
     1561 aaaggtgcac aaaaagtcaa agaaaataac gaaccacgtg tacaataaga agtaagaagt
     1621 cagttaggag gaatttaaaa tgggatttat ttgggcatta atcgtcggcg gtatcattgg
     1681 agcaattgca ggagcaatca cgaataaagg atcatcaatg gggatcatcg caaacatcat
     1741 tgctggttta gtaggttcag caattggaca agctttacta ggaacttggg gaccaacaat
     1801 tgcaggaatg gctatcattc catcattgat cggtgcaatc attttagtat tagtcgtttc
     1861 attctttgca cgtaaattat aagcaaattg aagaaaaact tatgttgaaa tgaagaacaa
     1921 atttgagaac cgtagcgaca ctcgctgcgg ttctttttta tatattttat atgaagtatt
//
LOCUS       DACDIH010000165         1946 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDIH010000165 DACDIH010000000
VERSION     DACDIH010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.168"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            203..1243
                     /locus_tag="IV281_002812"
     CDS             203..1243
                     /locus_tag="IV281_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP8781735.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          749 a          265 c          318 g          614 t
ORIGIN      
        1 tatttaatta taaataatat ttatttaaag tagttcttaa tgactttaaa ggtagtacat
       61 aattcatttt atttctgatt ttaggtagta tttaattaaa aaacagtttt aatgcgtttg
      121 cattttttat ttatctttgc tattttcgaa aagagtaggt aattttacat aaataaaaca
      181 acgtgaaagt agaggatcta caatgactga ttttaaattt tttaaggcgg atagagttta
      241 caacgaatta ttttatcaat ttccaaaagt ctttattgtt tctgacgaat acaaaaaaat
      301 gaaagattca actaagattg cctatatgct tttaaaagca agattagaga tcgcaatcag
      361 caaacggcaa atcgatgaag aaggtaatgt ttattttact tatacgacaa atgaactatg
      421 tagagtatta aactgccaaa aacaaaaagc gatagcaatc aaaaaagagt tggaatcctt
      481 tggtttatta ttacaaaagc agatgggatt taacaaacag ttagggaaaa ataatcctaa
      541 tagactatat ctagcagaat taaaagtctc agaaaatgat atctacttac tcgaaaaatt
      601 tgatagagag aatagggaaa acgttgataa atcagagggt atgaaaatca tacccaccct
      661 cgacgaaaaa tcagacgctg aatcccttgg ggctcaagag ggtatgaaaa tcataccgtg
      721 ccaaaacgtt gataaatcag agggtatgaa aatcatacca gaacttaata ataatatatt
      781 agacactaat agacacaata tagacactga aaaagaccgc ctacaagatc aattgttgtt
      841 agacaatttt gagacaatta tgacaaacga cagcattgct acgtttgtcc ctgaacgagt
      901 attaaatttg ataaaaacat tttcttcaag ttacagtgaa gctcaaaaaa ccgtccagac
      961 tattcataat gcaaagaaaa aagctgaaat agaaagtgat atttcgatag tttttgaaga
     1021 actcgatagt tattatgtca atgcagaaca agaattatac acgacactgt taaaagccta
     1081 tcaaaaatta aaaaccgaaa aagtcgaaaa tatccagaac ctgatttttg tctatgtaaa
     1141 aaattggttt atcgaaaaac caatagctgc taaagtatca agtgaaaaac gtttgaatta
     1201 tgcaagctcc ccaagcacta ttacgaaaga ctggttagag tgaaacttta ctgacaaagt
     1261 gattgttgct tcatggagta acattaaaat taaaaatttt ttattattct aattatatta
     1321 cgaatattac aaaataatag ctattttaat agaatttata agttaaaata ttatgataaa
     1381 aatcacaaac gtagcctttt acgtcataag tgttaaaaga aataattaat ttcaaatata
     1441 gaaaagggaa cgtatgtttg catactcctt ttttttgagt tataataata cgcgaagaga
     1501 gataatttca ttatttaaac aagctaaaaa tcttgatatt acttcttttc aggcaatacg
     1561 aatattattc gcttacattt tttgtataaa tttaattgtc tgctatgctt gtgatgaatt
     1621 gattttcgtt ttgcactttt ggatgataaa tttggacaat tgaaaataca aaaaaaagaa
     1681 aggcggtatg aactcggaag gctggtaacc ttcgtcagcg ttcatacacg gcattaagat
     1741 ttggccctta atgcgttaac ctttcagtgt tacggtatta cccgcaatgg atttagtata
     1801 acagatattc aaaagtgata ccactttttt tggctgtgtt aaggttaaaa atacgtgata
     1861 ttacgatgaa tctatgccta agcataatgt tataaaaatg tccgtgtggc tctttgccgt
     1921 atgacgaacg ctgaaattag agggaa
//
LOCUS       DACDIH010000166         1856 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.169, whole genome shotgun sequence.
ACCESSION   DACDIH010000166 DACDIH010000000
VERSION     DACDIH010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.169"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..728)
                     /locus_tag="IV281_002813"
     CDS             complement(<1..728)
                     /locus_tag="IV281_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781736.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQF"
     gene            complement(859..1581)
                     /locus_tag="IV281_002814"
     CDS             complement(859..1581)
                     /locus_tag="IV281_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781737.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            complement(1587..1832)
                     /locus_tag="IV281_002815"
     CDS             complement(1587..1832)
                     /locus_tag="IV281_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781738.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
BASE COUNT          525 a          324 c          256 g          751 t
ORIGIN      
        1 ataaactgat acggactgtc caaaattgaa taggttggtg atgacttcaa gaagacttca
       61 cctttttcac atgaaaataa accaattaat tcttcaattt catttacgat cagatattta
      121 ttagttaaaa ttttcccatt ttctttttct agctcgacaa tcgcttgatt ccatgtattt
      181 tgcactaaat cttgaatcaa tttcaatcta tccacttttt tcatctcata cagagcaatt
      241 tcagcttgtt taatgccttt taatttgcga aaggcatatt tgtattgtgt gtaggttaaa
      301 aagcctgtgt agttatacat gggctttcct gatgtcttgt agtcatttaa gcttggatat
      361 atttcagttt tccatttttc ttctgaacgt tgatcaataa ttcttaattt ttcaggtaaa
      421 tcaatttcat agtattcttc aattttcagc tcacgataaa gtagatgttt ttccttttct
      481 ttttcaatta caaaagaatt atttcccttc agtttagcta tttcttcact tgtgagacca
      541 agcctaattt tttctacttc acttctaagt tcacgaatac ttgttgcctt taaccacttc
      601 ccatttgcac cattattgta accggtaagt aactgtattt tataaattcc ttcaccatga
      661 agcaccttat atataccaac ctgattcttt tgcggaatat gagcttcatt tgcactgatt
      721 tctttcatta cgcattctcc tttataatct agttaattag cttgtgtgtt tcgagtagtt
      781 gagtaagaac gtttgtttca gaaatcattt atttgttatc acttaatttt aaaaaaatag
      841 ggcttacgcc ctaaagatct atttaacttt ggattgatcg tccataacag gctctgattg
      901 ttgatataat ttatatgctc ccaaatccat ataatattta gttgtacttt caatcatttc
      961 atcagttgta tttgttgtca ttcccattac atcccagtat agtgtgccgt taactttctt
     1021 tggtgttact actgcaactg ttggcgtatg atattgattg ttctcatcag ccagaccagg
     1081 aatgttttgt acccactcag gtacaccatc agtaatatct acagcaactg cgtaatactc
     1141 gtaagctggt ttatttatgg taatatcatg cattaatgtt ggagcataac taaatccttc
     1201 ttttatttct tgcatgattt ttgattctgc tttttgacaa tctggacaat caggtttgta
     1261 aagaacgata gtcagatttt tattttgcaa tggatcattc ccataatttt catcattttt
     1321 tactgtatta ataaagttgt tataaacttc atccggcttt tgacctttat taatgtcttc
     1381 gtatttttga tacttttcag cattattttc agtcattgaa gacattgttg ttgatgccga
     1441 tgtagtgctg tttttagtaa ccatcgtact actagacttt gtgtctttgt tattattagc
     1501 actattagtt gtaccacatg ctgtaagggt tattaatgct gctgtcgata acaatacaac
     1561 cttcaattta gttgatttca ttttattcac ctatcctttt ttcttttgtt taacaataaa
     1621 agaataaccc gtactatcca ataaaacaac agcatcaata tggaaaccca aaccatttgt
     1681 acaatccaag cataaagact aaataatagg tattgtggat ttttttgaat gattgtattt
     1741 gagatgccat ttcctacagt aaaatacatt gttacgaaaa taacaccttc aattatcaag
     1801 tgatttgttg gttgatttat aatttttttc atatttttac tcctaaacta aattta
//
LOCUS       DACDIH010000167         1821 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.170, whole genome shotgun sequence.
ACCESSION   DACDIH010000167 DACDIH010000000
VERSION     DACDIH010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.170"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..818)
                     /gene="dltD"
                     /locus_tag="IV281_002816"
     CDS             complement(<1..818)
                     /gene="dltD"
                     /locus_tag="IV281_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP8781739.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYT"
     gene            complement(821..1054)
                     /gene="dltC"
                     /locus_tag="IV281_002817"
     CDS             complement(821..1054)
                     /gene="dltC"
                     /locus_tag="IV281_002817"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP8781740.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            complement(1099..>1821)
                     /locus_tag="IV281_002818"
     CDS             complement(1099..>1821)
                     /locus_tag="IV281_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP8781741.1"
                     /translation="SGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFII
                     GYYFGQVLLPVVGRIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQ
                     TPINFNKPFLSWNIKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGY
                     FALFLLMGIWHGLTWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFL
                     TFQAVCFSFLIFSGFLDKLFFS"
BASE COUNT          586 a          367 c          292 g          576 t
ORIGIN      
        1 tgcgtataaa aatcgttttt caacccgaat ggattattcg tcgtttctcg ttctccaatt
       61 tgatttgcta aaatggataa ttctgtttct tggtattgtt ttggtaatcg ttttgactca
      121 tgatcgattt tttgctgtcg atctttcaac ccaatattgc tgaatacttc atcctctctc
      181 ttcagcatgt tccattggct gcgcagctga ttcaaagatt caggtaaagg tagcttccct
      241 tttttgatcg tttgcagaat cgccgtcaac gtatcattct cttttacttt tgagaaagtt
      301 aataatcttc tagctaaata gcgatcagca ggcgttactt tcttcattga aaacaaccag
      361 tcgtatgttt gaagttcaga ataatacgta ttaaagtaat cagtttttac accattttta
      421 acgaaccact gaggagaaat aataaagacg actttcttgt ttttcatagc atctcctgca
      481 gaacgcatca tcatatattg gcttaacgat tgtgtcccgg gtgctcccag caaaaatgga
      541 cgatagtttc gctggtattt ttctgccaga acagacggat gaaatggact gattctgctt
      601 aattcacttg agccaaaaaa aggaatatat tgtttttcag ataatgcata attttttatc
      661 gcatctcctt tcaacacgtt cactgacatg gaagaagagg cacgtctaat cgtttctgga
      721 ttagagatat ctacttgata ggaggaggta aaaaacacaa acagtaagcc aacagctaaa
      781 acaaccgggc cgaaaatagc aaaaagtttc tttttcatac tcaacccttc aatgcagttg
      841 cttgttgaat gattttattc ggtgtgctcc aatcctctcg ctcgaattca gatacaggga
      901 cttggatatc cagctctccg tctagctcga ctaataattg aacagatgct aaagaatcaa
      961 gcaacccttc atcaaataga tcaatatcac gattttttct tacttcatct gttcctgtta
     1021 tttcttctaa gattgataaa atttgttctt ccattttcct tttctccttt gttatataaa
     1081 ttaatatgtc actattcgtt agctaaaaaa aagtttatct aaaaatcctg aaaaaatcag
     1141 gaagctaaaa catactgctt gaaatgtcaa aaatacggct aagccatgcg taaatttgtt
     1201 agaaggcaac tgctctttat gcttcttctt aaatcggatc catgcatctg tcagacagat
     1261 cagacaagca tgatacagtc catatacgat gtagtaccac gtcaatccat gccatatacc
     1321 cattagaaga aacaaagcaa aataaccgat atttgacatt acgatcttgc ttttaaaaaa
     1381 ttttttcttc atcatgaaga acatcaatcg catatataca taatcacgga accaaaagga
     1441 cagcgtcata tgccaacgat tccagaattc tttgatattc catgataaaa aaggtttatt
     1501 gaaattgatt ggtgtctgat atcccatcat gtagcttgta ccgactgcaa ataagctata
     1561 gccagcaaaa tcaaaaaaca aatacatact gtacacgtac atataaccta ctaatgccca
     1621 tgaaatacca ccatgctgta aggcaatgcg tcccactaca ggaagcagta cttgaccaaa
     1681 ataataaccg ataatgaatt tatataaaaa accgacaaaa atattttgga cgccttttcc
     1741 caaaagatca acatatttat cacgaacagg cggatggagc tgatcttttt caaatctcct
     1801 atagcgatcg attggtccag a
//
LOCUS       DACDIH010000168         1795 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDIH010000168 DACDIH010000000
VERSION     DACDIH010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.171"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            530..1003
                     /locus_tag="IV281_002819"
     CDS             530..1003
                     /locus_tag="IV281_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP8781742.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1012..1239
                     /locus_tag="IV281_002820"
     CDS             1012..1239
                     /locus_tag="IV281_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8781743.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          595 a          289 c          270 g          641 t
ORIGIN      
        1 taaactaatc caatgcatct acttgcgctt attaagctat ttcagctaga tagtaccacc
       61 ccatgcgtca atgcttgatt ttccttttgt ataacattgt ataagcaata aaggacaaat
      121 aagtggtgat tacaccaaac cattgaataa gaaaaattct gatacgtaaa taaacatgaa
      181 cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg tcttttttct catacagttc
      241 tgttaactta cacttctctt cgctagataa taaaaaatcc tccaactgta ttgtccaata
      301 taatcccagg catctaaact agcattatag aagagacaca catagaatct aaaataactg
      361 gttttaaatg tttcttacaa tcaaaaaaag ctgttaaatg cactccacac cttcatactc
      421 tatcattttt tataaacgat atatttttat tgtttttcga taaaaaatag ttgttttaca
      481 ataattattc tgctatattt tagctatcaa agataaaaga gggataaaaa tgaaatttaa
      541 aacattgcta ttaaaatttg tcgttttagg tataagtgcc gtatttattt tgttcggcgt
      601 acttttttat actcagatcg ctacaagcga aaaaggattc aattttgatt ggcagaccac
      661 attatttgtt tctgtcattt actttgccct tctcctaggg atcgttattg cttatttctt
      721 attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt tccactcaaa gcttaaacat
      781 actaaaaaaa atcgaacgaa ccattttgtt tattggtttc gccttttttg gcttacttcc
      841 taaatttatg aaagtagcgg atttagacga tgctccaggt ttgatgttgg taggaatcgc
      901 tgtcgtattc attccttttg cagtatacac attaacaagc gtgctggaaa aactgcttga
      961 acatgcgatt ttacttaaga cagatcatga tttgacagtg tgaggtgcga catggcaatt
     1021 atcgttaatt tagatgtagt attagctcaa aaaaaaatga gtgtgactga attgagtgag
     1081 aaagtgggca ttacgatggc aaacatctct attctaaaaa atggaaaagc aaaagccatc
     1141 cgtttttcca ctctcgaaaa aatctgccaa gttttggagt gtcaaccagg tgatttgttg
     1201 acctataaaa atgactcgaa tactatacca gaagaataaa ataaagtaga acaaacagta
     1261 aataaaaaat aatctgaatg atagatgtgt gagactttta gaagttattt acgtctatta
     1321 ttcagatttt ttatttcgat tgaataccgt tttatctata aataagatat gtactgactc
     1381 atacaaaggg ggccttttct tctccatgta ctatcacttt tcctgataca actggtttcc
     1441 tgttacgttg tagttggatc tgcttttagt ataatacttt attcattcca ccagactgat
     1501 agcagtttga gcagtctaaa taatcatgta gtattacagg ccattctgtt tcataccttg
     1561 aagtgcttaa aacttcagct aatggcccat tgtgttaact cttactccat tcttttttag
     1621 taagctccca tatatactgt aaagccttct atatattatg tcatgttgct ttttaacatt
     1681 tactatgtag ttttgataaa atgatttgaa taacagtgat ggacaattct tttttatttt
     1741 taacattatt ttgataataa tgattattta tttaaaaata cattagataa cattt
//
LOCUS       DACDIH010000169         1783 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.172, whole genome shotgun sequence.
ACCESSION   DACDIH010000169 DACDIH010000000
VERSION     DACDIH010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.172"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..483
                     /locus_tag="IV281_002821"
     CDS             <1..483
                     /locus_tag="IV281_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8781744.1"
                     /translation="SLNIKSSYLHLLSDTLSSVSVIIGAILIRFFDIYWIDPVITLII
                     SIYILIEAIMVIKKAAAILIQSAPAIDYEKMEQEIEAIDGVKDVHHVHIWQYSEKIII
                     FDGHIDFEDQLLSEIEKIYPRIISLLKLKYGITHVTIQAETKAKDQKKLIFLNKDESY
                     "
     gene            531..1001
                     /locus_tag="IV281_002822"
     CDS             531..1001
                     /locus_tag="IV281_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP8781745.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            1086..1694
                     /locus_tag="IV281_002823"
     CDS             1086..1694
                     /locus_tag="IV281_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP8781746.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
BASE COUNT          602 a          256 c          347 g          578 t
ORIGIN      
        1 agtttgaata taaaatctag ttatttgcat ttgttaagtg atacactgtc ttcggtaagt
       61 gtgattatcg gagcaatttt gatccgtttt ttcgatatct actggatcga tccagttatt
      121 acactgatca tttcgatata tatattgata gaagcgataa tggtgatcaa aaaagctgcc
      181 gctattttga tacaaagtgc acctgctatc gactatgaaa aaatggaaca agaaattgaa
      241 gcaatcgatg gggtaaagga tgttcatcat gtccatattt ggcagtatag cgaaaagata
      301 attattttcg acggacatat tgattttgaa gatcagctac taagcgagat tgaaaagata
      361 tatcctcgta taatttcttt attaaaatta aaatatggta ttacacatgt gacgatccaa
      421 gcagagacaa aggcaaagga tcaaaaaaaa ttaatttttt taaataaaga tgaaagttat
      481 tgaaatatag agtatgataa ataagaattt ttaataaggt aggttggctc atgaagcagt
      541 atcgacagtt aaaaggatat ttagaagcaa aaggatattg ggagatacta gtctatctct
      601 tttttggtgg tttggctacg attgtaaatt ttgttagttt tgctctcgca cgccaatatt
      661 ttgatttaag tatggcctta tccaattcta tttcgtggtt ttgttctgta ttgtttgctt
      721 ttgtaacgaa caaactttgg gtattccatt caaaatcacc gaattttaca catgcactta
      781 ttgaatgcgg taagttcttc ttctaccgta tcctttctta cggattggac atgggggcaa
      841 tggtattgtt gatcaatgtt atgaacagca atgagtatgt ggcgaaaatc atcactcaaa
      901 ttattgttat ccttgcgaac tatattttca gcaagttgtt tatttttaaa gaaactgagg
      961 tttttgaaga agaagtaggt caaagtgaaa aagacgacta ggcataacaa ttgaagaatt
     1021 tccttctctt tgttactctt tgtaatgtag acattattaa gaaaacattg aggaggaatt
     1081 tttttatgac ttacacatta ccagatctac cttatgcata tgacgcattg gaaccttata
     1141 ttgacgaaga aacgatgcat ctgcatcatg ataagcatca caatacttat gtgacgaatt
     1201 taaattcagc aattgagaaa tacccagaat taggcgaaaa aacaatagaa gaattattat
     1261 ctgatatgga cgctattcca acagatatca agacagctgt acgtaacaat ggtggcggac
     1321 atgctaacca ttcatttttc tgggaaatca tggcaccaaa tgctggtggc gaacctacag
     1381 gagaaataaa agaagcgatt aatgaagctt ttggtgattt ttcttctttt aaagaagaat
     1441 tcaaaaaagc agccgctgga cgatttggtt ctggatgggc ttggcttgta atggaaaatg
     1501 gaaaattagc tattacctct actgcaaatc aagattctcc attgatggaa ggaaagacac
     1561 caattctagg tttggacgtt tgggaacatg cttattattt gaaatataaa aatgtccgac
     1621 cagattatat tgcagcattc tggaatgtta ttaattggga tgaagtaaac aaacgtttcg
     1681 aagcagcaaa ataaccattt cctccgtcat atcagtctta aaaataattt ggaaaataaa
     1741 agagcgaaag cttgctagcg ttttctaaat tgggtataat gtt
//
LOCUS       DACDIH010000170         1752 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.173, whole genome shotgun sequence.
ACCESSION   DACDIH010000170 DACDIH010000000
VERSION     DACDIH010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.173"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            123..362
                     /locus_tag="IV281_002824"
                     /pseudo
     CDS             123..362
                     /locus_tag="IV281_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
     gene            382..1602
                     /locus_tag="IV281_002825"
     CDS             382..1602
                     /locus_tag="IV281_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8781747.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1660..>1752)
                     /locus_tag="IV281_002826"
     CDS             complement(1660..>1752)
                     /locus_tag="IV281_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8781748.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          589 a          322 c          352 g          489 t
ORIGIN      
        1 agaaggcact ctcttcgggt tttcggtctg tactgaaatc aaggtattat tgggaatccc
       61 agcttaaatc atagataccg taagggattt tattctttat ttaaaacttt gcaacagaac
      121 cgcgccaaat attggattca ccagagttaa aggcacgaat tatgccaagc tcagaatttt
      181 tattgggtca ttcactccaa gcctttgatg agcaaggacg actcattgat gaagaacaag
      241 tcgcaaaatt acgtgaacta ttcaaagatt tcttactctt tgtcacaatc actagtcaat
      301 tgcaacatgc gcaccaagca aataaacgtg aagccgaaag cttttcatgg gaaacgattt
      361 aatcgagggg agcaaacaaa gatgaaatta ataggtatcg tcggatcagc agctgaactt
      421 tcatataacc gtaaattatt aacatttatc caaaaacaat atcatgaact atttaattta
      481 gaaatcattg aaatcaaaga tttacctctg ttcaatcaag atgatgatca aacaaatagt
      541 cctgctattc aacagctaaa tcgcaaaatc ttaatggctg atggggtaat tattgctacg
      601 ccagaacaca atcatacggt tccagcagga ttgaaaagtg cacttgaatg gctatcattt
      661 aaaatccatc cacttgaaaa taaacctgta atggttgtgg gtgcttctta ctacgatcaa
      721 ggaacttcac gcgcgcaatt acacttgcgc caaattttgg acgcaccagg cgtgaatgca
      781 ttagtgatgc ccggaaatga atttttattg ggaaaagcaa aagaagcatt tgaccaagaa
      841 gatcgcttga ttgatccacg aactgttgac tttttaggca gtacattagc aagttttgta
      901 aaattcatta aagtcgtgtc taccttagca gggcctaaaa agcttccacc agaagatttg
      961 catgcgacag gaacaattgc tacgacaata cccgatgttg atatgtcaga tgatcattgg
     1021 ttggaaaaag cttctgaagc agtcggtgcc gtttctggaa atacttacgt caaattaaat
     1081 cgtgggttat tgacagtgga tcaaatcaat tactttttag attcgatgcc gatggaatta
     1141 acttttgcag ataataataa ccaattttta tattataatt tgcgccaaga agcgaaagag
     1201 atgttagctg ctcgtgtgcc cggtcaggta gggaatccat tagccaactg tcacccagaa
     1261 aaatcgtata aaaatgtaga gtgggtaatt caacaactgc gaagcggagc aacagatgcg
     1321 gtacgtgttc atgtaccaaa acaagtgcca gaaaaatata ttgtgcataa ttaccaagca
     1381 atgcatgatg aaaatggaga gtatgctgga atcaatgaat acattcttga tttcaaaccg
     1441 atcattgagt ggtatttaca acaaacgggt caacaacttg ttggaaatga agatgcggtt
     1501 tcaagtgcat ctgttcatgg tggaaaaaca gatgcagtaa ccagcgccag cacacacgat
     1561 gaaactgcag tagatgcagt ttcgagtgct tctgtaaaat aaaaaggttc tgttgcaaag
     1621 ttttaaataa agaataaagt cccttacggt atctatgatt taagctggga ttcccaataa
     1681 taccttgatt tcagtacaga ccgaaaaccc gaagagagtg ccttcttttc gggttttctt
     1741 atataatcct cg
//
LOCUS       DACDIH010000171         1696 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.174, whole genome shotgun sequence.
ACCESSION   DACDIH010000171 DACDIH010000000
VERSION     DACDIH010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.174"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            125..391
                     /locus_tag="IV281_002827"
     CDS             125..391
                     /locus_tag="IV281_002827"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781749.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPQSFFMEFQIT"
     gene            412..>1696
                     /locus_tag="IV281_002828"
     CDS             412..>1696
                     /locus_tag="IV281_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8781750.1"
                     /translation="MWLLNICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIY
                     YRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIA
                     CMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTD
                     LQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTW
                     VCVTNLFPWFLFQKAPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGA
                     TVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERP
                     HLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVND
                     SYKKHGLVNVDVNAGENKIEITTKYTPTALMALWIS"
BASE COUNT          601 a          244 c          275 g          576 t
ORIGIN      
        1 aataaaaatt gattcttttc tagtatatca tactagtgct atgaaaaatt taacgcaaaa
       61 cttattaaaa aaagacaatt ctagtatact gaaaatagaa ttaaaaaagg agaacaacaa
      121 atctatgaaa ataagtaaaa aagcatttct tttagtagtt cttatcttac ttagtacttt
      181 atattcagta aattttatga gaaatgctca agaaatttat acaacaggtg atttatcttt
      241 tcatctttca cgaatcaaag ggttatcttc tatttttgaa gggccaatca actatacaac
      301 atttaacaat tatggagatg gactgaacta tttttatcct tttcttacaa taattcctca
      361 gtcgtttttt atggaatttc aaataactta atattaagtt atgttctata tatttggtta
      421 ttaaatattt gtacgattct gattagtttt tattatggag aaaagttttt taaaaagata
      481 gatgctgctt ttttatttag ttgtttatat acttttgcaa cttatcgaac agttgatatc
      541 tattatcgat ccgcaattgc agaagcaatc gctcttacga ttatcccagc tgttttatat
      601 tatgcttatg gattgatatt caatagagaa aaaaaagtca tcgctttatc tgtcagtata
      661 agtctacttg tgtatactca tgtcttaact acactattgt gtagtgcatt gattgcttgt
      721 atgattatag ttcgtttgtt tttaagcaag caaataaaaa acgatataaa atttgtggtc
      781 gtgaatttag taaaggctgt ttttttgact tgtgcattaa ctagctattt ttggtatcct
      841 atgttgcagc agatgaatta tcaagaaata cacagtccct tcaaaacaga tttgcagcta
      901 gaagcacttt ctatttcaga tagtatcatt caagcgttaa ataacgatat tacaacatac
      961 actatggggt tattaggatt ggttgcgttg atttttcctt tattcatact gaaagaaatg
     1021 gagaaaaaag aaaaaatcat ctatgttggc gtagtttcta catgggtatg cgtaactaat
     1081 ttatttccat ggtttttgtt tcaaaaagca ccgataagct tactccagtt tccttggcgt
     1141 atcttaggta tccaaatctt tttttcttca atgatcataa cgcttgtttt tatgaaaaag
     1201 actatgaata aaaaatattc atggttaatc attggcgcta ctgtggtgtt tctactcgtc
     1261 acgtctatag cagcaaaaga aaattacgca aaagttatcc aatcaaaaca cggtcatatt
     1321 gttatcaatg aagaaacact cccacgttat acaacatcag atatgggagg attatacgat
     1381 tacgccccaa cagaagcgat aaaagaaagg cctcacttag aaaaacatga agtaaaagta
     1441 ggagaatcgt gggaaaaagg gaagtataaa gctgcagata ataacattac ttacacagtg
     1501 gcaagcaaca aaaagcaaaa agtgacagta ccagtatatg cttatttagg tacacaggta
     1561 aaagtaaacg gtaaagtcgt aaatgactct tataaaaaac atggcttagt gaatgtggat
     1621 gtaaatgctg gagaaaataa gatagaaatt acaacaaaat atactccaac tgctctcatg
     1681 gctttatgga tcagta
//
LOCUS       DACDIH010000172         1648 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.176, whole genome shotgun sequence.
ACCESSION   DACDIH010000172 DACDIH010000000
VERSION     DACDIH010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.176"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(41..847)
                     /locus_tag="IV281_002829"
     CDS             complement(41..847)
                     /locus_tag="IV281_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781751.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQFAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTAENWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            complement(935..>1648)
                     /locus_tag="IV281_002830"
     CDS             complement(935..>1648)
                     /locus_tag="IV281_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781752.1"
                     /translation="SQNESGKTLIETMKKDYLNENDFPTWTNIEDATWDVVCTIYSPW
                     VTIADFVLDKIGGDTYPDAKSLATSDFKTFYGYLIGLIGNGIDLKVTPKQTPEEIEKE
                     KGEVQMAYANDREKVKLLEGKLSEEELGKMVQEINEDSNYFPELKVYQNDEDFFNEMF
                     SNSPWEAVSKTNLGEWNFQDDLVAFSNDGYLRSYSESEYRAELKTYETDILMSYLNLL
                     ESGQGELRDKVEELLKEEL"
BASE COUNT          454 a          347 c          199 g          648 t
ORIGIN      
        1 tagttttttt atcaaaaaaa tagagctttt cagctctatt ttattcaata tcattttgat
       61 caattaataa tcgtacactc caattaaccg agttttgatc actcaaatat ttataatctt
      121 tgtcatcaaa aaccagttgc caaactctgg tacgaacagt ttcttcgtcc acttcctcat
      181 ctgaagagta ttgcgataat gcttcttcaa ctatatactt tacaacttcc tcaaaatcag
      241 taggaacttt atcaataacc acttcatagt cttcttgttt tacgatattg ggatcaatat
      301 gttcatccaa acatacctca gtgagttttt ctttcacctc tgtatctctt tctgttttta
      361 ggtactctgc atagtcttcc ttagaccatt gtttttcaag atcatccgct atacttttaa
      421 taaaggattc tttatttaac caattttctg cggtatctat accttcaaac tcaaaagctg
      481 ttccttctat acgtgatttg acgaaagcat caattttctt gtattgatta ttcatttccc
      541 atgaaacaca ctcatcttct tgcgtaatat cataaatttt ttcggaaaaa ctaactaatt
      601 cattccacga tttatcctcg gcaaattgac ttagtttcga agcgataaaa ggaaattttg
      661 cttttaagtc atccatattc ttgatctcaa attcgatatt ttcttctcct acagctgaaa
      721 tccatgctga aagattgatt gcaatatcgc taaagtcttc tacattttcg acatttcctg
      781 tattatcttt tattactgct tctaaagcca tatcggttgt tgcactcatg gtttgatctc
      841 ctctcataat agagcaaaaa gccggcttaa ccgacttttt gcatggtacg ttttgtttgg
      901 tagttttctg cttgtgttta tgttgaaata atccctatag ctcttctttt aatagttctt
      961 caactttatc tcttaactct ccctgaccac tctctaatag atttaaatag ctcatcagaa
     1021 tatccgtttc atacgttttt aattcagctc tatactcact ttctgaatat gatcgtaaat
     1081 acccatcatt gctaaatgca actagatcat cttgaaagtt ccattctcct aaatttgttt
     1141 tactcactgc ttcccatgga ctattactaa acatttcatt aaagaaatct tcgtcatttt
     1201 gatatacctt caattcagga aaatagttgc tgtcttcatt gatttcttgc accatttttc
     1261 caagttcttc ttcagataat ttaccttcca ataattttac tttttctctg tcatttgcat
     1321 aagccatttg aacctctcct ttttcttttt caatttcttc aggtgtttgt tttggcgtaa
     1381 ctttcaaatc tattccattg cctattagac ctattagata accatagaac gttttaaagt
     1441 cacttgtagc aagagatttg gcatcagggt atgtatcccc tccaatttta tctaaaacaa
     1501 aatcagcaat cgttacccaa ggagaatata tcgtacacac tacatcccat gtagcatctt
     1561 caatattcgt ccatgtcgga aagtcatttt cattcaaata gtcttttttc attgtttcga
     1621 tcagagtttt tccactttcg ttttggct
//
LOCUS       DACDIH010000173         1631 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.177, whole genome shotgun sequence.
ACCESSION   DACDIH010000173 DACDIH010000000
VERSION     DACDIH010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.177"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..384
                     /locus_tag="IV281_002831"
     CDS             <1..384
                     /locus_tag="IV281_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP8781753.1"
                     /translation="FFVSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTT
                     GNLKGVATNLYDYCISKEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVS
                     ILFLIMALTQTFHLWQFYHTNRFTI"
     gene            complement(465..1184)
                     /locus_tag="IV281_002832"
     CDS             complement(465..1184)
                     /locus_tag="IV281_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8781754.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
BASE COUNT          441 a          338 c          260 g          592 t
ORIGIN      
        1 ttctttgtta gtttgacacc cgtccatgag catcccgcat tagtaacgat tttgattgct
       61 tttactgcat cgatccagtg ggtagctttt gataaaatca acgggcgtgc ctacactaat
      121 ttatttacaa ctggaaattt aaaaggggta gcaacgaact tatatgatta ttgtatttct
      181 aaagaaaaag cagattttga tcgatttttc cactatctaa gtgtcgttct tgcctttatc
      241 gcaggagcta ttgtccttgt tttctgtcat catctgtttg gcgcccattc gattttgatc
      301 gtttccattt tatttttgat catggccctc actcagacat ttcatttatg gcaattttat
      361 catacgaatc ggttcactat ttgatctcaa tgaatcaaaa aaactaagca agatgctgct
      421 cacagagaaa tatctgcgag tgagcaattt gcttagtttt tatattattt agttgtaaag
      481 attccctttg ctttcgggtt atttgtagtg tagcggatcg actgggcatc tgcctcttct
      541 gtcattttct ttgcccgcat cttgatttct tgtacgattt gttctccaca tgccggatcg
      601 gctacgaaat catatttgtc cccatcgatc actattttgg gacatatatc aaactgttca
      661 taccactctt cgtatcgtcg attgagttct ttatagtagg aatagagttc aggatcttgt
      721 tctagctgtt catattcccg gcctcttttt ttgattctcg ctaacatagt ttcaaaggat
      781 acacgaatat aaaccagtag atctggtttc ttttgtggcg aaatttcttc taactccttc
      841 agcatcgttt ctaaaagacc ttcatactgc tttacttcta cttcagaaac acgccctaaa
      901 tctgcattca aatgaaacag catactatct tcgtagatgg agcggtctag tacgttgtca
      961 tcatggctga aagcatcttt gattgccaaa aagcgtttgt tcaaaaagaa aatctgtaat
     1021 aaaaaagtat acttttctgg attagaataa aatagcggca atacttcatt atcttccacg
     1081 ttctcataaa aaggttttgt ttctaaatct tgggcaagca tttcagttag tgtgcttttc
     1141 cctgcaccga tcgttcctgc aacaatgatc acaattatcg gcatccttcc tgtaataaaa
     1201 tctgttcttc attttactac ttcatttata gaaataaaag ccttgacttt atctgctcct
     1261 taaaagctac ttctctaaaa gtagccaaaa gtaactccta ttcatgaaaa taaaggctgt
     1321 gacaaaagtc ttgtcgcagc ccttttttca aataaacagt attctcctgc caacacctcg
     1381 gtcacaaggc aatattcata aaacatgaaa agctgttttt taacattatt ccttgtaact
     1441 cagagctaaa cgggagactc acaacctctt tatgctctca acattttatc tgtttttatt
     1501 cttctattgt acttgatttg cgtacaacta actgccagtg cagattatga gcggtatcat
     1561 tgtcttctac tgcgcatttc atgacacgta tcatcacact attttcatag attttttcta
     1621 tttttccttt t
//
LOCUS       DACDIH010000174         1627 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.178, whole genome shotgun sequence.
ACCESSION   DACDIH010000174 DACDIH010000000
VERSION     DACDIH010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.178"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..241)
                     /locus_tag="IV281_002833"
     CDS             complement(<1..241)
                     /locus_tag="IV281_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8781755.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYI"
     gene            complement(353..1492)
                     /locus_tag="IV281_002834"
     CDS             complement(353..1492)
                     /locus_tag="IV281_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP8781756.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          423 a          342 c          303 g          559 t
ORIGIN      
        1 caatgtattc tttttcctca gcaggaaaaa tgatttcggt tctcccacaa cgtcgcttat
       61 tttctttgta ttgttcaaag tattcaagtg ctgttccacc acatttgaga aagttataga
      121 cattgtaaat tgtttggcgt ccacgtttaa gctgcttcgc aacgatagca accggagttt
      181 cttgatgaaa atatgcttct atcattacaa gctcgtttgg tgtaagatgg gtataagtca
      241 tttatgttca ctctccttgt atgctttagc gggtattaca attggagtgt aacataaatg
      301 gcttttttat ttgtctcgct taattataca aacggcgttt attaaaaaaa tcttacgcta
      361 agtaggataa aataattcct tgatattttt cgatcatatc caaataatta tcgatttcaa
      421 catactcatc tacttgatga ggcaaagtaa cgacaccagg cccaaagacg acaaagtcga
      481 aggaatggtt agctttcgta aattcggctg catccgttgt tgctgccgct ccaactaaag
      541 gtaatggctg gctgaattgt tgctgaatgc aatgaatcaa aggagaatct ggatcagctt
      601 tgacaggtat tttgttgtaa tcgatcatca attccaaatg atagtctgtc tcttgattca
      661 attcattgac gattgattgc agcaatgcaa tgattttatc atttgggtat tctggaatcg
      721 atcgaatgtt cccttggagc tgcgcatggc ttgggatgct gttgacttga ttcccgccac
      781 tgatcaatgt aacattatgg attgtttttc ctaggacagg attctcgatt gtttcagcga
      841 ggtgattcat ttcagcattt gctttcgtaa tgaattcatt taggtgattg attgcattat
      901 aaccttggtc tggcatagag ctatgcgctt cttttccgtg cgaggtgact gtatagttga
      961 ttgatcccat gtgcgtatac atcaaactgt aattggttgg ctctccgatg atcaaggcat
     1021 ctaagtcatc tacatagcct gctttcgtga gctgttcccc acctagttca cccacttctt
     1081 ctccgacagt ggctaataat ttgactgttc cgttaaatgg ttttcctgac tctttcagtt
     1141 cgatcatggc aatcaccatt gctgccaagc cactcttcat atctgtagca ccacgtccgt
     1201 acaaacggtt cccatggatt tctgctgcaa agggtgcata ggtccaagac gattcatcac
     1261 cagccgccac gacatccata tgcccagata ggcctaacac ttttcctgat tggccctttt
     1321 ggtaacgagc aatcaaatta tctctaccat ctctgtaaga aacaatctct cctgttatgt
     1381 catgctttgc caaaagctta ttcaaatagg ctgctacttc accttcgttt ccattcactg
     1441 atttaattcg gataatctct tgtaaaatcg cgattttttc ttcttttttc atctaatcct
     1501 ctcccttgcc atttcttttt attgtatttg agtgtgctcg aattttcaaa taggagcaac
     1561 caaagtaaaa atgaagaaag agagccactt tatgaaaaat atgctgctac ttccctccta
     1621 ttttatt
//
LOCUS       DACDIH010000175         1616 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.179, whole genome shotgun sequence.
ACCESSION   DACDIH010000175 DACDIH010000000
VERSION     DACDIH010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.179"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            207..500
                     /locus_tag="IV281_002835"
     CDS             207..500
                     /locus_tag="IV281_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781757.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            493..789
                     /locus_tag="IV281_002836"
     CDS             493..789
                     /locus_tag="IV281_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781758.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            860..>1616
                     /locus_tag="IV281_002837"
     CDS             860..>1616
                     /locus_tag="IV281_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP8781759.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETV"
BASE COUNT          609 a          223 c          319 g          465 t
ORIGIN      
        1 tattcatgtt cgtaatgaat ggcaattggc agatagtcaa ttattatttc aagaaaaata
       61 acattttatt ttcttatgtt acttataagt agttttacat gctttttgac tttaaaaata
      121 catgtgatga tacatgttat atgaattatc aatgtaccca gtaatatgct attttcatta
      181 tgtaaaaaat aacagggggt gtgtagatgg agattgatga aacttgtttg agtaactctt
      241 ataaaagtat gaatgacttt tttgaggaaa tggaactgga ttcagaattg ctctatcaat
      301 tttatcttgc ttttcgaggg caacagattt cttttccaat gaaaatgtac gatcgtgagt
      361 tagttagaaa aagaatcgaa aacatgatag aacaggaaaa aactgtagat atccgtcaat
      421 tgacagaagt ttatggtttc tctgcaagat ggattcaaaa agtaattaaa caaaaagaaa
      481 ggagacgagt acatggctaa gctgattgta ttaggtggtt taatttacgg tggatattgg
      541 ttatataaat atatgcttgc gcatccgaat atagataaag gaaaagtaat taagacagtt
      601 ggggtaatca tagttggact catagctcta actaagaaaa ataatactaa tagcccaaaa
      661 ataatgagaa cttatcgctg tagaggatgt ggaagggaaa tttctactag ttttaatcca
      721 tctgaaagcc ctcaaggaag agatcgttgt catgctggct atcgacattc ttgggaaagg
      781 attaactaaa tgaacaccat tgagattaat tgactgataa aataagtacg cgagggagaa
      841 attgaaaggg ggaaataaaa tgaaaaaatg gttgttatta ctaattggaa tggggatttt
      901 attcactggt tgtacatctg agaatacgag gactaaagag actgtagaaa ctgatacaac
      961 aattgaggct aaaaaaagta aaacgcaatc aactgaacaa actacaacag aaatgaaaca
     1021 atctgtggaa gcaagtacaa gtgaaagttc aatcattgaa tcttcttctg aagaggttaa
     1081 aaatactttg tgggatagtg agaaatctac tcaactagca atttttgtcc cacaatgggg
     1141 aaagacatta ggtcaagaat ataaaagcta caatcctcag aacaatgtaa gtctgtatgg
     1201 tacgccttta ccaagtgcag ttattgatgg aaattggaaa atggcaatca atgaagcacc
     1261 agttaccgta caatggtcag aagatggaac tggtcatgca gatttcaatt tagtagcagt
     1321 ttattctgat gtagaaactg gagaatattt agggcagcat gtatatttct ttggctttca
     1381 aaatggacaa cctaaagttt atttaacgca acaaaatcaa ggcaatgata aaaactacat
     1441 gtacttcaat gaaactcaga accaacagtt aaaacaaggt ttcattgata ttgtgaatgg
     1501 acaaactcct caaactccag tagtagaaga atctactcaa caagcaacaa ctacaacgac
     1561 acaatctaca agtagctcag caatttcaag ttgggaagct gctaaagaaa ctgtac
//
LOCUS       DACDIH010000176         1611 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDIH010000176 DACDIH010000000
VERSION     DACDIH010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.180"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(629..1501)
                     /locus_tag="IV281_002838"
     CDS             complement(629..1501)
                     /locus_tag="IV281_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8781760.1"
                     /translation="MIYGAIEAGGTKFICAVSTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQRKSKKYGHITSTPKLAWQNFNFVGTLKQHFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMLSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIASYIVTPELGDSAGI
                     IGGLALARKAIRNK"
BASE COUNT          498 a          355 c          252 g          506 t
ORIGIN      
        1 catcttttgc caactctttg tcttaaattc gaaacatata agcagagcta cgaacaccaa
       61 gactgtttcg gctctgtttt ttgcatcaaa ttcactctta tatagctgat aatctcggct
      121 ctttaaaaga aattattttc gcttcatttt attataaaaa ggctaggaac cttgatgtta
      181 tcaggttcct agcctttatt taagtattac cccacagacc cttccatata gattttcaaa
      241 ttacacacta gtttaagata aaacaaacaa ggaaaaactt accatttact caaaaaatta
      301 actcatataa gtacattaga atgtaaaata gactggtcaa aatctggtca aaaaagtaga
      361 tgtgacacaa gttcgcattc aaagcgatga tccatgattt cgtaatggtc aattatatgg
      421 atcaaagcca cgcgacaaga gcgcaatgga tgtctctacc gattggttga ataaacgaaa
      481 taaatagaat gtgaaaaagg atcggacaaa tgttccagtt tgctcgatcc ttttttattt
      541 gaattcattt tcgctttatc aaacatcatt tgattactca gttaaatact tttgacatga
      601 gtaccaatga aacatttatt cattaaaatt acttatttct tatcgccttt ctagccaatg
      661 ccaatccgcc gattattcca gcactatcac ctaattcggg cgtaacaatg tagctagcaa
      721 ttggtggtat ttcaacatat ccatttataa gctcaacaaa tttatcctga acttttttct
      781 tcatgtgttc ttgtttcatt acaccaccgc caaaaataat tctatctggc gaaagcatca
      841 gtgttgtatt gtatgcacat tgggcaaggt aaaaggcttc aatctgccaa aacgaatcgt
      901 cagccaataa attctggcct tttacaccca atcgtttttc aatcgctggt cctgctgcca
      961 tcccctctaa acaatcttgg tggaagggac agtttcctga aaaattgtcc tttggatgtc
     1021 gcttgatcaa catatgcccc atttccggat gactgaaccc ttccacgaaa gtcccattga
     1081 acaaggcacc acctccgata cctgttccca cagtgaagta tgcaacacct gctaaccctt
     1141 tgccagaacc agcaacatat tcaccatagc aagccgcatt aacatctgtt gtccattcaa
     1201 tcggaatatt gaaatgttgc tttagcgttc ctacaaaatt gaaattctgc caagcaagtt
     1261 tcggtgttga tgtgatatga ccgtatttct ttgatttacg ttgaatatca attggaccaa
     1321 aacagccgac cccgattcct gccaattgtt ccttaaaagg tttaaaaaaa tcaatgacct
     1381 gcttcattgt ttcaactgga gtcgttgtgg gaaaactgac tctttccaaa atcgtcaatt
     1441 cctcagtact gactgcacaa ataaatttag tccctccagc ttcaattgcc ccatatatca
     1501 tcataggctt ctcccttcga atatgtgtaa aacacttcat taacgcggca agcgttctcg
     1561 atcagtgaaa tgacgtttag tatttcctga agcttctgtt gaataccaat a
//
LOCUS       DACDIH010000177         1594 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.181, whole genome shotgun sequence.
ACCESSION   DACDIH010000177 DACDIH010000000
VERSION     DACDIH010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.181"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..138
                     /locus_tag="IV281_002839"
     CDS             <1..138
                     /locus_tag="IV281_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8781761.1"
                     /translation="GYIRPDHGRMIWGETGRPGYGLYDRALGAVYLNGIWEALEKGAS
                     K"
     gene            212..>1594
                     /gene="uxaC"
                     /locus_tag="IV281_002840"
     CDS             212..>1594
                     /gene="uxaC"
                     /locus_tag="IV281_002840"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019239247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8781762.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRN"
BASE COUNT          531 a          230 c          347 g          486 t
ORIGIN      
        1 ggctatattc gtccagatca tggtcggatg atttggggag aaaccggtag acctggttat
       61 ggattatatg accgagctct aggtgcagtt tatttaaatg gtatttggga agcgcttgaa
      121 aagggggcga gtaaataatt atataaggga gtttatttag aggaaaaatt ttctcttcta
      181 aaaactaacg gaaggaaagg agtgtttaaa tatgttttta gataagaact ttttattaga
      241 gaatgaaata gctaaaagac tttttcataa atttgcaaaa caccaaccaa ttatcgacta
      301 tcattgtcat ttagacccag catatttgct agaaaataag caatacaaga atttgactca
      361 ggtatggtta aatgataaag gagctggaga tcattataaa tggcgattga tgcgagctaa
      421 tggtatagaa gaagagttga tcagtggtcc aggtgatgat tatgaaaaat ttttagcttt
      481 tgtaaaaaca gtggagaagg cgccaggtaa tcctattttt gaatggtctc atttggaatt
      541 acaacgttac tttgatattg atttgacaat ttctgagaag aatgcaccag aaatttggcg
      601 acgtgctaac gaaaaaattc aaacggattc ctatcgcccg aaagaattgt tggagatgat
      661 ggatgtgaaa gccatctgta caacggatga tcctacagat gacttgtggt atcatactga
      721 gctttctaaa caggataatt ctttcaaggt attacctact ttccgtccag ataaggtctg
      781 gagtatcaaa tcatcaggct tttcgaacta tattttaaaa cttagtcaaa cagcggctat
      841 accaatcaaa gactttaatg ggttgaagca agcattgagc caacgagtgg atcattttca
      901 tcaaattggc tgtcgattag cagatcacgg tttgaatact tatttttatg aaatagcaga
      961 tgaaaaaata ctcgacaatc tgttaataaa agctttagca ggaaaaacgg atttccattt
     1021 atcagaaatt gcacaatttg ttacaggtat tcaattacat ttgatgtctg agtatacaag
     1081 atatggttgg acgatgcaaa tgcatttaaa tgctttaaga aataattcaa gtatattaaa
     1141 ggaacaaatt ggagtagatg tcggtggtga ttcgatgggg gatcaagcta gtttgacaca
     1201 aaatgttgta gcattgttcg ataccgctga aaagagtggg tgcttaccaa aaacaatttt
     1261 atattcatta aatccaactg attggcttcc actagcaaca gcaatgcaaa gcttccaagg
     1321 gggtatgatt caaaaaatcc aattgggatg tgcttggtgg ttcaatgata cagctgaagg
     1381 aatgcgtcaa cagttgacta cgatggcaca acaaagtttg ttgtcgaatt ttacaggtat
     1441 gttgactgat tctcgaagtt ttctaagcta tcctcgccat gagtattttc gtcgagtatt
     1501 atgtcaatta ctaagcgagt gggttaaagc aggccgaatt ccagatgatg aaatgtatct
     1561 tagtcagata gtggagaata tttgttatcg taat
//
LOCUS       DACDIH010000178         1543 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.182, whole genome shotgun sequence.
ACCESSION   DACDIH010000178 DACDIH010000000
VERSION     DACDIH010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.182"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(44..1423)
                     /locus_tag="IV281_002841"
     CDS             complement(44..1423)
                     /locus_tag="IV281_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8781763.1"
                     /translation="MMGNLVLSYNIYYFFITFLTMVVSYFSMKQIKNNRYISLLFSII
                     YTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTH
                     LLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQE
                     LKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAFIFIKKLTADDLFIFF
                     LGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYTMSIVIYYIFSTKNNS
                     WKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAENYNHTDYANKESIYHP
                     DMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTPIYRYKGQVASINGKL
                     VQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTLILFLLYRFTFSKQKQ
                     PRREVQHSH"
BASE COUNT          593 a          218 c          246 g          486 t
ORIGIN      
        1 cctgtttatg tgtacagcta aaaagtcttt cttaaataaa aatctagtga gaatgttgta
       61 cttctcttct cggttgtttt tgtttagaaa aagtaaatcg ataaagtaaa aataaaatca
      121 aagtaacgat agataaatat cttgaagcaa ttgctagttt tgtgtactga taagtaataa
      181 cgactttatt tatacctgga ggaatagtta attcagttgt gccaaactta gataattttg
      241 tttgtactag tttgccatta atagaagcca cttgcccttt ataacgataa ataggtgtcg
      301 tcaagacagt atttttatta ttcgaattat ctaattcaat tgtaagtttg ttactcataa
      361 aatgagttgt tggatttatt tcctggttac ctagtaaata ttgatcatta ttaatcatat
      421 caggatgata gattgactct ttgtttgcat aatcagtatg attgtaattt tcagctatag
      481 caacagcgtc ttcaggtgct ataagtgtta atgaatcttt gttagcatgg atagaattgc
      541 ttactcctga gagatgcaat attagtgcaa gaaacgtaaa gaccattatt ttccatgaat
      601 tatttttagt agaaaaaata taatagatca ctatactcat cgtataggct ataaataaag
      661 aagaaaaacc atttaaccgc cagacaaatt gtagactctt aactggtgta tggttaaaaa
      721 gttgccaagg aaataaattg gttgaacaaa aaagaacaaa tacaccaaga aagaaaataa
      781 ataaatcatc agcagttaat ttttttataa aaataaaagc accaatcaat ccgagaaata
      841 agaataatcc aatagtatag ttgttatagt tattatttaa aatatgtgtc agcatatcac
      901 taggagccat accacttaat tcttttccca gtgggacttt caattcttgt gctctagatt
      961 gttgaataaa aggaattaag aatcccgcac ttaaaaaaaa ggtgaggacg gtagctttta
     1021 aaagacttaa gagtctagcg attttttggt cccaaaaata aaaagaaaga aacaacgttc
     1081 ctccaataaa tacagaaacc atagcgacag aaagtaaatg agtatacact actagcgtca
     1141 tgccaataga caaccagtac catttttggt aatctctaat ataaatttcg taacacccca
     1201 ttagtatcaa aggcaaaaat gttaaagcga cagcttctcc caatgaagca cgatggaata
     1261 tgtcaatggc acgataggcg gaaaatgtat aaataatcga aaataacagc gaaatataac
     1321 gattgttttt tatttgtttc atagaaaaat aagaaacgac cattgttaaa aaagtgataa
     1381 aaaaatagta gatattataa cttaaaacaa gatttcccat cattttataa aataaaaaag
     1441 ctggataaag agtaagccat ggataaaaaa tattcatcat tgttccatga tgatcgaagt
     1501 tattgaagtt tactggagaa ctccatacat tatctagtcc tat
//
LOCUS       DACDIH010000179         1527 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.183, whole genome shotgun sequence.
ACCESSION   DACDIH010000179 DACDIH010000000
VERSION     DACDIH010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.183"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            12..317
                     /locus_tag="IV281_002842"
     CDS             12..317
                     /locus_tag="IV281_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781764.1"
                     /translation="MPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVITLLLLV
                     MMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINPLIFSR"
     gene            481..735
                     /locus_tag="IV281_002843"
     CDS             481..735
                     /locus_tag="IV281_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781765.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            805..1050
                     /locus_tag="IV281_002844"
     CDS             805..1050
                     /locus_tag="IV281_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8781766.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            1126..1491
                     /locus_tag="IV281_002845"
     CDS             1126..1491
                     /locus_tag="IV281_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781767.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
BASE COUNT          435 a          251 c          275 g          566 t
ORIGIN      
        1 gagtgattat tttgccattt attgcgattt tattcgattt agttgcggca gcagcttatt
       61 ttctccaatt aaattatcaa acctcaggaa ttctgtttat tggaatgatt tttcaaggtg
      121 tgattaccct tttgcttctt gtcatgatgc tcagctataa aggaaaaaga tatgcccgta
      181 ttcaaacaaa agtatttgtt aaatatgttt ccattcgcta tgggatcatc attctttcct
      241 ttttagtgaa tgcgatcgtc ctctttttat atgtattgaa ttacttagat atcaatccat
      301 tgattttttc tcgatagata agacacattc agcggaacag ttcctgctta tcagcagaaa
      361 actgttctgc tttttcattt ttcattgatc tattcttctt ttattttcct agcatttcat
      421 ttgtgatgta tgcaataaca tgttatcttt tattatatga atcgttgagg agtccgttac
      481 atgattacgt tggtgagagt tttgttttgg gtgcctgcgg tcgcactggt cgcaagtatt
      541 gtttatctta tgaattggaa caaagaacgg ttttatttag ctattttgac tcttccggct
      601 atctacttta tgtggaaagt ttttaattac aactactttg aacccgatag tgtttttata
      661 gaagaattat caggactggt tttgtcttta ttgatcgtca ttctttattt gatacgttta
      721 aataaaaaac attgagtact attgattgca tgtgtagctt tctatagtca ttataactat
      781 tctactataa tgaaaggtga ttaaatgagt atcgaagaag ctattttact ggatacttta
      841 cgttgtcgtc tgaatttctt aagaactggc cgatctttat ctcaagtagc tgtttctagc
      901 gcgttaagta ttcctttatt caaatatcgt aagtacgaag aaggagaaga aattccaagc
      961 atcattgaat taatctatat cgctgacttt tacgatattt ccgttgatta cctaattggt
     1021 cgctcagaaa cagcaagcaa actgaagtga ttttttccag ccttggtgct ggttttttca
     1081 tttaacgtct aaggttaaca atctatatat aaaggagaca tttctatgcg attatggcat
     1141 gaaaaattga tttcaaagct tcctcgtcaa cagcttttag gacaacatcg ggaatgctgt
     1201 gcattaagag gaaatggctg gggaaagaaa catgctactg tggattatgt ttttcactat
     1261 tctccttata gattattcca atatcatcag cttgtcatgg atgaaatgga aaaacgtggt
     1321 tacagcccag caccagagtg gaaaaatcct atgtatcgtg gcaaatcatg cgaagcattt
     1381 tttgatttac ctactgtaga gcgcaacgtg ccgatttatc cggaacacga tgaagtttat
     1441 ctagcagaat gtttagcaaa tttggagcaa aaaggaattt atcttaccta atataactag
     1501 aaaagccttc tatctcctct tacttca
//
LOCUS       DACDIH010000180         1511 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDIH010000180 DACDIH010000000
VERSION     DACDIH010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.184"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            265..1395
                     /locus_tag="IV281_002846"
     CDS             265..1395
                     /locus_tag="IV281_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8781768.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
BASE COUNT          424 a          246 c          328 g          513 t
ORIGIN      
        1 acgtcgaaag tcgccaatgg gtaaaacaga aactgttgga ttaaggcttt ttccaatagc
       61 taagaatgtt ttctttacgt tgtatagtgc taaaactcgg acgatgagat gatgctaggt
      121 cagaattctt gttcgaaaag gtttctgacc ttttctttat atttttgtga ctgacttatt
      181 tgatgagaca aactcgctcc tcacctgaca agtggattgc tgtgtgagga ggagatgcgg
      241 tgaaaaaaga tcgattggtg gattttgggt ccttatttga ctttgtccat gatcggtctt
      301 ttagaagtat acagtgccag ttcttatcgt ttattgcaag ccgatgaaaa tacaaaaagt
      361 ctattattgc gtcaactaat tttcatattt ttgagttgga gcgtgatctt cttagctcgt
      421 tcagtcaaac tacactattt acttcaccct aaaatagcag gatacggttt agccttatcg
      481 attttctttt tagtattagt aagaataggg atattcggtg tcactgtcaa cggcgcacaa
      541 cgttggatct ctctgtttgg cattcaattc cagccttctg aactggcaaa tctttttttg
      601 attttttatt taagctggtt ttttcgtgac ggaaatagta gcccaaaaga tctaaaaaaa
      661 ccattcctga ttacagtagg tataactttt ctgattttat ttcagccaaa gattgctgga
      721 gcattgatga tcctttcgat tgcgtgggtc atattttggg cagcggcggt tccatttaaa
      781 aaagggatct atctaatcgt tactttttct gcattgctga ttggagcagc aggcggggta
      841 ttatatttag gaaataaagg ttggcttcca cagatgttta atcatgctta tgaaaggatc
      901 gcgactttaa gagattcttt tatcgatagt catggtgcgg gctatcaaat gacccattcg
      961 ttttatgcac tatataatgg tggcatattt gggcggggat taggaaatag tatcacaaaa
     1021 aaagggtatc ttccagagac agaaacagat tttatttttt cgattattac agaagagctt
     1081 gggctaatcg gtgcattgtg tgtgcttttc ttattgttct cattatgcat gagaatcttt
     1141 tgtttaagca gtcgctgtaa aaatcagcaa gctggattgt ttcttttagg attcggaact
     1201 ttgctattcg ttcagacgat aatgaacgtg ggaagtatcg cggggctgat gccaatgact
     1261 ggtgtgccat tgccatttgt tagctacgga ggaacaagtt atctgatcct ttctttagga
     1321 atcggaatca cgttgaatat ttcttcaaag atacaggccg aagagttggc gctctatcga
     1381 cctgaaaaac aataaagaga aactgagaaa ctctcagttt gaattatcat aaagtacaat
     1441 cttttatttt tcaagcgaga ggttctttta gaaagaggaa ttcgcaatga aagtatctca
     1501 aaaaatcata t
//
LOCUS       DACDIH010000181         1489 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDIH010000181 DACDIH010000000
VERSION     DACDIH010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.185"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            195..920
                     /locus_tag="IV281_002847"
     CDS             195..920
                     /locus_tag="IV281_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781769.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            910..1419
                     /locus_tag="IV281_002848"
     CDS             910..1419
                     /locus_tag="IV281_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781770.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
BASE COUNT          408 a          288 c          242 g          551 t
ORIGIN      
        1 aaattattgt cgctgctcct cttttcgaat aaacagtgtt caaaagcttg catctacggt
       61 aatagaatag ggcagcttct tcacaacctc ttgcttttta caaaatattt tctggtttta
      121 attcgtgatt tcactaaagg aatttcatac tttattcata ttcattttct atacttttta
      181 tagaggtgaa aagtatgaaa ataatccgct atgctactgc tcgattaatt tattacaaaa
      241 agcagacaat actctattgt ttattttcaa gcttttctgc ttttttattg atagcatgcc
      301 tgactcttta ccattcgcaa tcggctttag ccaatcaagt ccaagatcgg ctttcattta
      361 ttggcgcttc tgcatctaca gagcttcttc cggctttaga attagctagc cgattttata
      421 taaaaggcat attcagttta ttttttctat ttgccttcct ttttatcttt ttcttttact
      481 attcagtcag gcaaaacaag caggaattga tcaattggcg cctgactggt ctttctaaaa
      541 ccaaattatt cctttttatt ttctggcagc tgttctttcc tctctttctt tgttgtatct
      601 tggttttatt gtggatcgtc agcttccagt cagtttatga agcgcttctt caaaggatca
      661 attttcttgt cttaaagact actgaactgc cagatatccg tgatatcttg acttcggtaa
      721 gcggacttac aattccgatg gatcaacaga ctttttttac catcgatttc caaaatgatc
      781 tttttttcac agatatactt aagggatttg cccaaactct tattgggctc agtggatgct
      841 gtctcattct tagtctattc cctttcactt tttttatcca tcatttgcaa agtaggaggc
      901 ctggacgtaa tgaaacctga atatttgatt catgctgaac acttgaccgt tattttgtgt
      961 cctctcgaaa aacagcataa aatattggag cacttgaaaa aagaggttct tgttgcaaaa
     1021 ctggataatt ttgcgatcat ccaaaaaaat tggccgatgt ttccttatct gaatcttaaa
     1081 gaccatgtcc tgttggatgt accggaaaaa gaaatcaaac aagatcgttt ggcctatcaa
     1141 gaaaaacttg atatctctcc ttccttgctt aattgtgcgg cagctgagtt gacgtctttt
     1201 gaaaaagtaa agctgcaatt acttcatgcc ttgctttcca agcgaaacaa tattgttatt
     1261 gaagatacat ttgatgaact tagtgtattg gagatccaag agctactcca cttgctttct
     1321 tatttagctc atgaagaaaa tcaaggtatc ttactcttta ctcatgatgc tactattgct
     1381 caaagtcctt atattgatcg tttagagccc gcgggctaaa tcaatcattt tatagtaaaa
     1441 acaagcggta tagaagatcg attcatgatc ttttataccg cttgttttt
//
LOCUS       DACDIH010000182         1489 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDIH010000182 DACDIH010000000
VERSION     DACDIH010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.186"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            8..1201
                     /locus_tag="IV281_002849"
     CDS             8..1201
                     /locus_tag="IV281_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8781771.1"
                     /translation="MRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLTSSQQKGIV
                     LQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGIISYFYPVF
                     TLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEEEQQKIGTF
                     FKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGELG
                     KTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPIKD
                     VPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPRYS
                     QVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKSFL
                     QKMFV"
     gene            1214..>1489
                     /locus_tag="IV281_002850"
     CDS             1214..>1489
                     /locus_tag="IV281_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP8781772.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMA"
BASE COUNT          528 a          232 c          306 g          423 t
ORIGIN      
        1 accagaaatc cgtttcccag gatttacaga tgattgggaa cagaggaagt tgggggactt
       61 cgttgtagat tatgttgaaa agacaagtgt acaaaatcaa tttcctatgc taacttcatc
      121 tcaacaaaaa gggatagttt tgcaagaaga ctattttgca aatcggcaag taactacaga
      181 aaataatatt ggttactttg tactacctag aggatatttc acgttccgta gtcgaagtga
      241 taatgatgtg tttgtgttta accgtaatga cattattgat agagggatta taagctattt
      301 ttatcctgta tttactttaa aatctgctga ttccgacttc ttcttaagac gtattaacaa
      361 tggaatccaa cgacaattat ctattcaagc agagggaaca ggtcaacatg ttctgtctct
      421 aaaaaagttt aaaaatatag tagcaatgtt tcctagtgag gaagaacaac aaaaaatcgg
      481 caccttcttc aaacaacttg acgacacgat cgctcttcat cagcgtaagt tagatttgct
      541 gaaggaaacg aaaaaaggat tcttacaaaa aatgttccct gaaaacggag caaaagtgcc
      601 agaaatccgg tttcctggtt ttacggaaga ttgggaagag cgtaagttgg gagaattagg
      661 taaaactcaa tcgggaattg gatttcctga tgccgagcaa ggtggttctg aaggtactcc
      721 attttttaaa gtttccgaca tgaataacat tggtaatgag tatgaaatga gaaatgcaaa
      781 tcattatgta tctaatgagc aaattgagcg taaaaaatgg aagccaataa aagatgtgcc
      841 agcgattata tttgctaaag ttggcgcagc aataatgctt aatagaaaac gtttggtcac
      901 aagccctttc ttaatagata acaataccat ggcttattta tttaataata cttgggatat
      961 ctattttggt aaaatattgt ttgaaactat aaatttgcca agatactccc aagtaggggc
     1021 attgccttct tataacagtt ctgatattga gaatatcagt gttaaggttc ctgtaaagga
     1081 tgaacaacaa aaaatcggta ctttcttcaa acagctagat gatacgatcg ctcttcatca
     1141 acgtaagtta gatttactaa aagaaacgaa aaaaagcttc ttacaaaaga tgtttgtcta
     1201 ggagggaaga agaatgaaaa ttagtcaccg tgaggaagca gaagtcgaag aacagttgat
     1261 tcgtgtactt ggagaagggc ataatcagtg gacgtatcgt ccggatctaa aatcagaaga
     1321 agacctttgg gtcaatttac gccaaaaaat tatctcaaat aatcaagcag aactgaatga
     1381 ttttcctttg accgataaag aatttgaaac gatcaaaacg gaattactgc tgcgaacgaa
     1441 aaccccattt gatgctgcga aatggttaaa aggtgaaaat gggatggct
//
LOCUS       DACDIH010000183         1474 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDIH010000183 DACDIH010000000
VERSION     DACDIH010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.187"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1328
                     /locus_tag="IV281_002851"
     CDS             <1..1328
                     /locus_tag="IV281_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8781773.1"
                     /translation="KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEK
                     GYSLKDSKGQFFSVVKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQ
                     NGQRFQDSIFTIAEAWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDL
                     LMMGLEDLDIIHTKKHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVD
                     KADIMKVTTSLNPLHTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENP
                     GIIDPKQFIDEVINKRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENL
                     TFIPIAIAAWLRYLLAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQE
                     ILENKEIFGLDLYQVGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1331..>1474
                     /locus_tag="IV281_002852"
     CDS             1331..>1474
                     /locus_tag="IV281_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8781774.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIK"
BASE COUNT          522 a          210 c          306 g          436 t
ORIGIN      
        1 tcaaaaaaag acttttggca gtaacggctg atagtttata ttgcaatccg aagaatatag
       61 cggactatca aagagtggtt aaatattttg aagatcctaa attacagttt acaacgttta
      121 ctattactga aaaaggttac agtttaaaag attctaaagg gcaattcttt tcggtagtca
      181 aagctgatat caaatctgga cctagcaatc ctcaacatac gatgagtatt gtaacatcct
      241 tgttatatag cagatttaat gctggagagt tgccgattgc gatggtttct actgataatt
      301 tctcacagaa tggtcaacgt tttcaagata gtatttttac gattgcagaa gcttggcaaa
      361 aagaaggata tgtttctgaa aaatttattg cttatttaaa ggataaagaa aaagtgactt
      421 ttccttggag tatgattgat cgaattacgc caaatccttc agagaatgta aaggaagatc
      481 tattgatgat ggggctcgag gatttagata tcattcacac aaaaaaacat accaatattg
      541 ctgctttcac taatactgag gtagttcatt atttagttat tgaagatagt tttccaaatg
      601 gccggcctaa attggaaaag gctggtgtga ttatgacaga tagagagacg gttgataaag
      661 cagacatcat gaaggttacg acaagcttga atccacttca tactgctttg gcagtaacag
      721 gttgtctgct aggatatcaa tcaatttcag cagagatgca ggatgatgat ttggttggtt
      781 taatcaaagg aataggttat ttagaaggtt taccagtagt tgaaaatcca ggcattattg
      841 atccaaaaca atttattgat gaagtaatta ataaacgttt gcctaatccc tatattccag
      901 atacaccaca acgaattgca acagatactt ctcaaaaagt agcgattcgc tatggtgaaa
      961 caattaaaaa gtatgtcgaa aaagacggaa atgcagaaaa tctaacattt attcctatcg
     1021 ctattgctgc ttggttacgt tatttactgg cagtcgatga tcaaggggaa aattttgtgc
     1081 caagtcctga tccgttattg gtagagttgc aggataaatt atcagtattg aaattaggtg
     1141 aacaagatcc ttcaaagatt cattgtgcgg tacaggaaat tttagaaaat aaagaaatct
     1201 ttggtttaga tttatatcaa gttggggtag gaaagaaaac tgaaaaaatc ttttgcgaaa
     1261 tgctagtagg tcctcaagca gttcgtaaaa caatcaataa atatataaca gaagttggag
     1321 gaaagtaact atggaaatga cttttagatg gtatggggca aatgaagaaa aaatctcact
     1381 acaagaaatt cgtcagatcc cagcaatgaa aggaattgtt ggaacattaa tggatatacc
     1441 agccggagag gtttggccga gagaacgaat taaa
//
LOCUS       DACDIH010000184         1466 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.188, whole genome shotgun sequence.
ACCESSION   DACDIH010000184 DACDIH010000000
VERSION     DACDIH010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.188"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            44..496
                     /locus_tag="IV281_002853"
     CDS             44..496
                     /locus_tag="IV281_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781775.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            510..1199
                     /locus_tag="IV281_002854"
     CDS             510..1199
                     /locus_tag="IV281_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781776.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
BASE COUNT          525 a          191 c          308 g          442 t
ORIGIN      
        1 tagatatata ttataaattt aataagaaag cgaggtgaga atcgtgaaaa aaacgatact
       61 aagtattttt tcagtattta caatattaat tgggggctat tttcttttga atttagattc
      121 tgttaattca aacgctcaaa taaaagatca aagagtagct gaacataata ataaaaaaat
      181 gagtttgacc aaaaatatct attctatgtc agatgaagaa ttgtataaag aatttggtga
      241 tttatccggg gaacagttag aagaaagtat tccaggaatt agtgactcag ataaaattgt
      301 tgtcttgtct gctggtgggc aattaatggg agatggcaaa tccgaattag ttagttataa
      361 acaaaatcac agacagttta ttgatgttaa tactgattcc aacgctattt cgattaagga
      421 tctaaaagag cttttaaata gtaaaagaaa tgaattaaag cagataaaag aaactaattc
      481 agtggaatta gagtaaagga agattcatta tgaaaaagat ttttggtgtt tctgttgggc
      541 tacttatgat gttaacattt ggaaatgcag tatatgctgc tgagaaagaa gttatttcag
      601 atccagagca gtacgcctca cgatactttg ctgattttga agaagaagat aaagtacttg
      661 cattgccaga aggtggtttt ttaaaagggg aagctaaaat tgtatctatg gataatccag
      721 atataattca aggttcatat aattcagaaa ctgatcctgc ttcagtttca gtgacagaag
      781 caaaaaaaat tgttgctgaa aaattcaaaa atgatattga aactactcca attatgcctc
      841 gaggagcatt tccacccgat gttggtacta gcggacacct tcttgtacta gcaggaggag
      901 gaagctataa atcacagcca tttagtggtg cgggatggcg ttttggaggt atgggtgtaa
      961 aaccagctga tggtacaggc aactttttac gttggaaatc ctacattgac gatggaagag
     1021 taggaaatgt aaatcaagcc aacgcaacta aatctggttc tattcaagga atggcattgt
     1081 atgctcgtga aggattcaaa tactttgcac caaacgggcc tggttggaca atgaatatag
     1141 gtatttatta cacttataat cctgttgcag gtacgtatta tgttgttcaa aatatttaat
     1201 aaaacagcac aaagagattt ctttgtgctg ttttattatt tttagttgtt gacaaacact
     1261 aaaatataaa atataatcaa cacataaacg aacgtatgtt cggcaagaaa gcaggggatt
     1321 tttatggatg agatggtttt aagtactcag aaatggctaa acaagaaata cagtaatgtt
     1381 acagggtttg ataaggttcc agaaaatggg cgaacagggt ggcctacaat ctatggactt
     1441 attgaaggac tacaagtaga acttgg
//
LOCUS       DACDIH010000185         1415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.189, whole genome shotgun sequence.
ACCESSION   DACDIH010000185 DACDIH010000000
VERSION     DACDIH010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.189"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            90..1382
                     /locus_tag="IV281_002855"
     CDS             90..1382
                     /locus_tag="IV281_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8781777.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          460 a          288 c          256 g          411 t
ORIGIN      
        1 atctttttta ttttccaaaa catatagaaa agacaccttg aaatccttta gaattaattt
       61 ggcgaaccaa cccaaaagga ggaatcaaga tgtcttacac tcaccttatc aaagaaacat
      121 tagatattct agacttatca gttactttta acgaaaattg tctcacaaaa gaaaaataca
      181 aagggcaaat ctgtcatatt tatcgtggaa acttgattta tacagcccaa gaatgcatcc
      241 actgtaaaca ccagattgct tcagatattg tgcgttgggg tacaacaacc gtccggttgt
      301 taatgaacga tgtttctgaa taccgtacct acctcgaatt gaaaaagcaa cgcttcaaat
      361 gcaaagcttg tcagcggacc tttgtcgcag atacttctgt tgcgaaaaaa cattgtttca
      421 tcagtgagaa agtccgctgg tctgtcgtga ctcgtctgaa aaaaaatacc tcaatgacag
      481 agatcgccgc tcagaaaaat ctatcggtct cttctgttta ccgtattatg aagcgatttt
      541 atcgtccgtt gaatcctttt cggacaccgt tacctaaggt actttgtttt gatgaattca
      601 aatcagttcg gggtgtttca ggtgctatga gtttcatcat gatggatgga caaacacagc
      661 gcttattgga cattgtcgag aaccgacaac tgcctttttt aaaacgttat tttagtcatt
      721 tttcacgaga aattcgtgaa gcggtcgaat ggatcgtgat tgatatgtac gctccttatg
      781 tatcgctcgt taaaaagctg tttccaaaag cacagctgat tattgaccgg tttcacatcg
      841 tccaacatat tggtcgtacc tttcgcaatc atcgaatcaa agaaacgaac caactattaa
      901 aaagcaaaga acaaaaacac tatcaattag gtaagcaatt aaaaagatac tggaaacttc
      961 ttcaaaaaga tgaacgcaag cttgattata ctcgacgcct ttggcgacct ggcttcaaag
     1021 ctcatttaac agagaccgat attgtggatc gcttactgaa agggagccct gctttgcgtg
     1081 taggctatca gttatatcaa gactttcttt acgctgtaaa ggaacgagac tacgtatcgt
     1141 ttgaagaact acttaccaat aatatcatgc ttcccgaagg ctatcagacg accttgagaa
     1201 cgttccagaa atttctgcca caaatcaaaa atgctttaca acaaagttat tcaaatggcc
     1261 cacttgaatg tctgaacaac catatcaaag tgttaaaacg aaatgcatat ggctttcgta
     1321 gcttttataa tttcaaattg cgcatcatga ttcgtcatgg caacgcattg attttcaact
     1381 aaaaaagatc aagaggaact gatccacttg atctc
//
LOCUS       DACDIH010000186         1409 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.190, whole genome shotgun sequence.
ACCESSION   DACDIH010000186 DACDIH010000000
VERSION     DACDIH010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.190"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1027
                     /locus_tag="IV281_002856"
     CDS             <1..1027
                     /locus_tag="IV281_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP8781778.1"
                     /translation="PQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDAFALGKYHTGS
                     NLCLWKACSGLAEIFEALEQLSLSERYKDIACKLKESICKQMTIDGTFGEQFLEGIGD
                     EEKTTYSVKNYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMPFYQFLDNKDP
                     LYQNTMTFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSDMHNHQAFVTRLEEL
                     ICLADLDGSWWWWPYRLEAKQGEVVRDFGCGKCGWASGLFVSIMISQYFGLKFKKGVL
                     QIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKKILHVEKGKTI
                     YIQRRKR"
     gene            1024..>1409
                     /locus_tag="IV281_002857"
     CDS             1024..>1409
                     /locus_tag="IV281_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP8781779.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPT"
BASE COUNT          427 a          237 c          333 g          412 t
ORIGIN      
        1 tccgcaggtt ctactgaatg cttcaagaat tattgaggac atattagctc aacgtcggtc
       61 aggggaacca atgctttttc catctgtttg gttatcagac gcctttgcac ttggaaagta
      121 tcataccggc agcaatcttt gtctttggaa ggcctgtagt ggacttgcag aaatttttga
      181 agccctagaa caactgagtc ttagcgagcg atacaaagac atcgcctgta aactaaagga
      241 atcaatttgt aaacaaatga ccatagatgg tacttttggg gagcaatttt tggaggggat
      301 cggtgatgag gagaaaacaa cctattcagt gaagaactat caaaaaccga ttcttgagca
      361 agggttaatc tttttaagtg atgtgattgt ggatgggaaa atcaatttat tgatgcatga
      421 tggagaagaa tcagacacta cgttgatgcc tttttatcaa tttttagata ataaagatcc
      481 tttatatcag aacaccatga ccttttctgc tagttctttc aatccaactt atagcgagga
      541 aatcaaaggc atcacttggg gattagaatc cggcgccact tttcctggct ttatcactgt
      601 tttaatgagt gacatgcaca accatcaagc cttcgtaacc cgattagagg agctgatttg
      661 tttggcagat ttagacggtt catggtggtg gtggccgtat cgtttagagg ctaaacaagg
      721 agaagttgtg cgagactttg gctgcggcaa atgcggctgg gcatcgggcc tttttgtcag
      781 tataatgatc agtcaatatt tcggattgaa attcaaaaaa ggagtattgc aaattgatcc
      841 tgttgataat ttaacgtttt cttggaaaaa tttgaagttc ggtcaagcct tcatttcgat
      901 tgaatgtacg caatccgagt tgagtatttc aaatttgtca gaaaagccat tgaaaataag
      961 tgatacgaaa aaaattcttc acgtagaaaa gggaaaaacg atttacattc aaaggagaaa
     1021 aagatgagta tactaaaaaa tattacccaa tttaaaaaaa tccagagtcg agcaatcact
     1081 cccgaaaatc caacaggcga gaaaggtaaa gctgcgatgt tgccaagcga gctaggacca
     1141 agccgcaaag gtcgtggcgc aattccgctg acttctggtg aagaaactgt gattgcggat
     1201 attactggaa cgggaataat tcgtcatatg tggatgacga ttcgtgatcg aacagaattt
     1261 ggcagttttg ttttacgtga tttaatctta agaatttatt gggatgggtc ggatagcccg
     1321 gctgttgaaa gtcctttagg ggatttcttt tgtaatgggt ttggagagcg ttgcgaggtg
     1381 aactcgttgc ctatagtggt taatcctac
//
LOCUS       DACDIH010000187         1409 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDIH010000187 DACDIH010000000
VERSION     DACDIH010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.191"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1027
                     /locus_tag="IV281_002858"
     CDS             <1..1027
                     /locus_tag="IV281_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP8781780.1"
                     /translation="PQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDAFALGKYHTGS
                     NLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPFGEQFLEGIGD
                     EEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMPFYQFLDNKDH
                     LYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNPQAFVTRLEEL
                     ICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQYFGLKFKKGVL
                     QIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKKILHVEKGKTI
                     HIQRRKR"
     gene            1024..>1409
                     /locus_tag="IV281_002859"
     CDS             1024..>1409
                     /locus_tag="IV281_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP8781781.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPT"
BASE COUNT          425 a          243 c          328 g          413 t
ORIGIN      
        1 tccgcaggtt ctactgaatg cttcaagaat tattgaggac atattagctc aacgtcggtc
       61 aggggaacca atgctttttc catctgtttg gttatcagac gcctttgcac ttggaaagta
      121 tcataccggc agcaatcttt gtctttggaa ggcctgtatt ggacttgcag aaatttttga
      181 agccctagaa caactgagtc ttagcaagcg atacaaaaac attgcctgta aactaaagga
      241 atcaatttgt aaacaaatga ctatcgatgg tccttttggt gagcaatttt tggagggaat
      301 cggtgatgag gagaaaacaa cctattcagt gaagcactat caaaaaccga ttcttgagca
      361 agggttaatc tttttaagtg atgtgattgt ggatggaaaa attaatttat tgatgcatga
      421 tggagaagaa tcagacacta cgttgatgcc tttttatcaa tttttagata ataaagatca
      481 tttatatcag aacaccatgg ccttttctgc tagttctttc aatccaacct atagcgagga
      541 aatcaaaggc atcacttggg gattagaatc cggcgccact tttcctggct ttatcactgt
      601 tttaatgagc gagctgcaca accctcaagc tttcgtaacc cgattagaag aactaatttg
      661 tttggcagat ttagacggtt cgtggtggtg gtggccgtat cgcttagagg ctcaacaagg
      721 agaagtcgtg cgagactttg gctgcggcaa atgcggctgg gcatcgggtc tttttgtcag
      781 cctaatgatc agtcaatatt tcggattgaa attcaaaaaa ggagtattgc aaattgatcc
      841 tgttgataat ttaacgtttt cttggaagaa tttgaagttc ggtcaagcct tcatttcgat
      901 tgaatgtacg caatccgagt tgagtatttc aaatttgtca gaaaagccat tgaaaataag
      961 tgatacgaaa aaaattcttc acgtagaaaa gggaaaaacg attcacattc aaaggagaaa
     1021 aagatgagta tactaaaaaa tattacccaa tttaaaaaaa tccagagtcg agcaatcact
     1081 cccgaaaatc caacaggcga gaaaggtaaa gctgcgatgt tgccaagcga gctaggacca
     1141 agccgcaaag gtcgtggcgc aattccgctg acttctggtg aagaaactgt gattgcggat
     1201 attactggaa cgggaataat tcgtcatatg tggatgacga ttcgtgatcg aacagaattt
     1261 ggcagttttg ttttacgtga tttaatctta agaatttatt gggatgggtc ggatagccct
     1321 gctgttgaaa gtcctttagg ggatttcttt tgtaatgggt ttggagagcg ttgcgaggtg
     1381 aactcgttgc cgatagtggt taatcctac
//
LOCUS       DACDIH010000188         1371 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.192, whole genome shotgun sequence.
ACCESSION   DACDIH010000188 DACDIH010000000
VERSION     DACDIH010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.192"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..444
                     /locus_tag="IV281_002860"
     CDS             <1..444
                     /locus_tag="IV281_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781782.1"
                     /translation="IDFMIEHLTDASSFRLADKDMEVLPWIYNKLNQEQFNKLLYNLN
                     GNNQFYKNSNFPPFISNLLDFYNQKFQVNLRKHYSGLLYPNAVTMDQGFQLKDKELKK
                     LYELAESKSDMYLDIIDNIINKSEDYLRKEINSEKELLNYVHKIK"
     gene            complement(701..1036)
                     /locus_tag="IV281_002861"
     CDS             complement(701..1036)
                     /locus_tag="IV281_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781783.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
BASE COUNT          467 a          231 c          174 g          499 t
ORIGIN      
        1 atagatttta tgatcgaaca tcttaccgat gctagttcat tcaggttagc agataaggat
       61 atggaagtat taccatggat ttacaataaa ttaaatcagg agcaatttaa caaattacta
      121 tacaacctaa atggaaataa tcaattttac aaaaacagta attttccgcc ttttatttct
      181 aatctgttag acttttataa tcagaaattc caagtgaatt tacgtaaaca ttatagtggg
      241 cttctatatc caaatgcagt gactatggat cagggttttc aattaaagga taaagaatta
      301 aaaaaactgt atgaattagc tgaatcgaaa agtgatatgt atctagacat aatagataat
      361 attattaata agtctgaaga ctatttaaga aaagaaatca attccgagaa agaattgtta
      421 aattatgttc acaaaataaa ataatttttt ataacacaag atgcactttt atagcgtata
      481 tttactttca aattaaacta attagttata ttctcacaat agcatttaat aataaatata
      541 ctgtcgcttg atttatatat ttccctcttc tttctttaga atctctcttt attttgatat
      601 aataaaatag aaatcgcacc aaacaatgaa atggcggaaa acatttcatt gaaaaaagga
      661 ttgaacataa tgttcaatcc ttttttatag ttataatcaa ttaacattcg gtgtgtgaaa
      721 ttgaatcttc gatgctttca catggaaaat tattgattcc ttcttcactc atttcccatg
      781 tagtatagaa aggagttaac tgtcctccat actcctgaat aataaaatat tttccatcat
      841 tgatatggtt acaacagtac actccaatag ttggatactc gactactaaa aattggtctg
      901 gatcaaactg ttcttttttc aacatttctc gtatttgaaa caaatgatcc ttctctaagc
      961 aatcaggtaa ttcctttatt ctttgcgtta ctaatgaccc ctctatcgct ttccattcat
     1021 ttaaatattg tttcataaca attcattcct tttatgttaa ctatttttcc taggaaattg
     1081 ttttaaaacg ccactctccc agaaaacgta agatttggtt agctggaata taccttccag
     1141 ctaaccacaa ttttccatta ctatattgta agcttgcttt cacttcataa aaattgctga
     1201 tatactcttt tcaaatcgaa ctcttcctat ctgagaaggg tatttcttgc gctagcccct
     1261 cataagttcc atcttcattt tcccaaattt ctacctgaaa cactgaatta ttggctaatt
     1321 tttgttgcaa gagataatag ggttcttctt catacttttg atcgacttct t
//
LOCUS       DACDIH010000189         1358 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.193, whole genome shotgun sequence.
ACCESSION   DACDIH010000189 DACDIH010000000
VERSION     DACDIH010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.193"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..125
                     /locus_tag="IV281_002862"
     CDS             <1..125
                     /locus_tag="IV281_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="manganese ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8781784.1"
                     /translation="GYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKKERKL"
     gene            122..1072
                     /locus_tag="IV281_002863"
     CDS             122..1072
                     /locus_tag="IV281_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8781785.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            1087..1320
                     /locus_tag="IV281_002864"
     CDS             1087..1320
                     /locus_tag="IV281_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP8781786.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
BASE COUNT          541 a          167 c          259 g          391 t
ORIGIN      
        1 ttggttatag ttttaacata gcagcaggat caagtattgt tttaacagcg gcattcttat
       61 ttgtaattag ttttctaatc tcaccaaaac aaaaattttt aagaaagaag gagagaaagt
      121 tatgatgaga aaatggaaag cagtactggg aagtctgggg attttgattg ctctttttat
      181 attcggggca tgttcaacaa atagtaaaga caaagataca gtggcttcga acgaaaaatt
      241 aaaggtagta gttactaatt cgattttagc agatattact gaaaatatag caaaagataa
      301 aattgattta cacagtatcg tacctattgg gaaagatccc cacgaatatg aacctttgcc
      361 tgaagatgtt caaaaaactt caaaagcaga tttgattttt tataacggtg ttaacttgga
      421 aactggagga aatgcttggt ttacaaaatt agtaaaaaat gcgaacaaag aggaaaacaa
      481 agactatttt gcagcaagtg atggcataga tgttatttac ttagagggtc agagtgagaa
      541 agggaaggaa gatccccatg cttggttaaa tttagaaaac ggtattattt acgctaaaaa
      601 tattgaaaaa cagttagcgg aaaaagatcc tgataataaa aaattctata aagaaaatct
      661 agataagtat attgaaaaat tggattctct agacaaagaa gctaaatcta aatttgcttc
      721 aattccgaat gataaaaaaa tgattgttac aagtgaagga tgctttaaat atttctcgaa
      781 agcgtataat gtgccttctg cttacatttg ggaaatcaac actgaagaag aaggaacacc
      841 agatcaaata aaacacttag ttgaaaaatt acgcacaaca aaagttccct ccttattcgt
      901 agaaagcagt gtggacgata gaccaatgaa aacagtatca aaagatacca atattcctat
      961 ctattcaacg atttttactg attcaattgc agaaaaagga caagatggtg atagttacta
     1021 tgcgatgatg aaatggaacc tggataaaat tgctgaaggc ctttcgaaat aatttggagg
     1081 gtaggtatgg tacaaattaa aacaactact atactaattt atttactagc aattcaggaa
     1141 ttatcaaaaa gaaaaaagaa attaaaaaat aatgatttag caaaagttct aagtgttagt
     1201 ccagcatctg taagtgagat gctgactaaa cttagatatg aggactattt agatatagga
     1261 tttcaattaa ctgataaagg gaaaaaattt attgacgatt acaaaaaagg ttttaagtaa
     1321 aaggctttat ttttagggag gataagaaaa tggctatt
//
LOCUS       DACDIH010000190         1348 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.194, whole genome shotgun sequence.
ACCESSION   DACDIH010000190 DACDIH010000000
VERSION     DACDIH010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.194"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(124..1272)
                     /gene="tnpB"
                     /locus_tag="IV281_002865"
     CDS             complement(124..1272)
                     /gene="tnpB"
                     /locus_tag="IV281_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP8781787.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          389 a          238 c          213 g          508 t
ORIGIN      
        1 taacgcttga agtgggagct tcttgggaat agagcatact tgatagcgta tttctttact
       61 aacagcggtg tctcttattt accaagctat ccccgtagtt cctacggttc ttgatgattg
      121 gatctaagcc aatcccatag aatgtagtct tagaccttcg gtcagaatat tgatactagc
      181 attgatatca cgatcatgat gagtatgaca aacaggacaa gtccattctc gaatttcaag
      241 agatttcttg ccatctttat gtccgcactc tgaacaaatc tgactagacg gaaaccattt
      301 atctactttg atgatttttc gtccatacca gtcagccttg tattgtaatt tagtcacaaa
      361 ctttgaccaa gaaacatcag aaatactttt agctaattta tgatttcgca acataccttt
      421 tgtgtttaag tcttcaatac agataatatc gtgatttttg atcatttctg tactcaactt
      481 attcagaaaa tcagtacgtt gattcattac tttttcatgc aatctagcta ctttgcgttt
      541 ttgtttttga taatttttag cttcaaatag attggtacct ttatttttag ccaataaagc
      601 acgcctagac aatttgcgtt gttcacgctt tagtttcttt tccattttgg acgtgaattt
      661 attattatca attttttgtc catcagaaag aatcgcaaag tccataatac ctaagtcaat
      721 accaattgtg gaattagttt taggtaattc gataatttct tctttacaca acaaagaaat
      781 atagtattta ccgctagaat gacgtgatat tgtagcagat ttgataatcc cttttggttg
      841 tctatgaagc ttaattctta ctaatgactt caatttagga actttgatga atttactatc
      901 aatcaaagca actgtaccat tttgattatt cgttgtataa ctctgaacag gatttttctt
      961 acttttgaaa cgtggaaatc caacggattt atcccgaaaa aaattcttgt atgctttatc
     1021 taaattaagt tgggcattgg ctaaagcaag gctatcaatt tctttcaaaa acggaaattc
     1081 tttcttgtat ttagctggtg tcggaaaagt cattttttta gaagaatcat ttttaacttc
     1141 ttcatacgcc ttttttcggt catcaagcat tagattgtag accttacgga cacaaccaaa
     1201 agatttggca aagaagattt cttgttcctc tgttggatag attctgaatt tgtatgcttt
     1261 tagttgttcc ataaagttca cctactttct atttatgatt gtttccttga ttttggatat
     1321 acttcttaat cacatcaatt ggtgcgcc
//
LOCUS       DACDIH010000191         1318 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.195, whole genome shotgun sequence.
ACCESSION   DACDIH010000191 DACDIH010000000
VERSION     DACDIH010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.195"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..761
                     /locus_tag="IV281_002866"
     CDS             <1..761
                     /locus_tag="IV281_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8781788.1"
                     /translation="KIPEENIQALRKFDENGGIVTVATGRNSISAKDIFSELGIGGYL
                     ISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAKISIIASRETQDDQLTFD
                     ESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALTDKSEEKLQKLQRDLQAD
                     GIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMAFGDQENDIAMLNYSGLS
                     IAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            801..1298
                     /locus_tag="IV281_002867"
     CDS             801..1298
                     /locus_tag="IV281_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8781789.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
BASE COUNT          470 a          229 c          252 g          367 t
ORIGIN      
        1 gaaagatacc agaagaaaat atccaagcac ttcggaaatt tgatgaaaat ggcggaatcg
       61 taaccgtcgc taccggaagg aacagcattt ctgctaaaga tattttttcc gaattaggaa
      121 tcggtggcta tttgatttct tccaatggtt cactgattgc tgaaatgaag gatggaaaaa
      181 tggcgaaagt tctcagacgt tcaaaaatag atagcgatac actgagaaaa gccttcttct
      241 atgctcaaga agcgaaaata tccatcattg ctagccgaga aacccaagat gatcagctga
      301 cattcgatga atcaaatcta gtcaaagatg acccttatta tgaacatttc aatttacaga
      361 accattcttt tgatcaaatt ttaaatatgt tggaagatcc ttctcttagc tatcttaaat
      421 tagcattaac agataagagt gaagaaaaac tacagaagct acaaagagat ctgcaagcag
      481 atggtatcca atcagtattc tctgatccgc atttcttaga aatcacgcct acaggtatca
      541 ctaaagctca ttcacttgtt tttctggcgg attaccttgg aatcgaatcg agtgacatca
      601 tggcttttgg cgatcaagaa aacgacattg ctatgttgaa ttacagcggg ttgagtattg
      661 cgatggggaa tgcacagcga caagtaaaag aaattgcaaa ccaagtaaca ctgacgaatg
      721 aagaagctgg tgttgcttat attttaaaaa acttctatta aaaagaataa aatgacgata
      781 tgaatgtagg aggaaagaaa atgttagtta ttattgatat gcaaaatcat attcttgatc
      841 caaacagcga attttatatt ccagagtcag atcaattggc agaacgtatt gctcaaagat
      901 tagctaaagc acgccaaaat aatgaatata ttctcttcac acgggatata ccagttgaac
      961 tgaaggacaa agaagaagaa cgagaagatt tgcaattgat ccctcttctt tctccacaag
     1021 aaaatgaacg agtgattaaa aagtattact ttacgattcc acctgaaact ttagctgaaa
     1081 tccaggaaac tttatttgaa agcaaagaag aacaaaaaga gatcgaagta gtcgggattg
     1141 aaacaaactt gtgtgtactc tctaatctga taggcctgca gagtgctttt cctgaagcag
     1201 acttttttgt tgatccaatg ttagtctcca gtagaaaaca cggagaatcc gctttagagc
     1261 tgttgaaaga tttcaacgtc tctatcgtcg aatcataaaa atatctatct tcatttac
//
LOCUS       DACDIH010000192         1312 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDIH010000192 DACDIH010000000
VERSION     DACDIH010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.196"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(156..1297)
                     /locus_tag="IV281_002868"
     CDS             complement(join(156..1069,1069..1297))
                     /locus_tag="IV281_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8781790.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
BASE COUNT          323 a          263 c          278 g          448 t
ORIGIN      
        1 gacacgattt gtgagaattt tttcgagatt ccagcgggtg agtatctcat cgtttcgacc
       61 agcggtcaaa ccaaaaccgt ttgaccgctg atcgaagcga tgaagttgaa accctgtgga
      121 aatctcgcca aaaaaatgta agtggtagct aggaactaag cttgcaatgc caactcctcc
      181 atctgatttg gtgttaaata gttgatacta ctatggattc gcttgcgatt gtaaaatcct
      241 tcaatgtatt gaaataacgc taaataagca gcttcaaagt tttcatacgc ttttgatgga
      301 tagacttctt cttttttgag tgaggcgtga aacgattcga taccggcatt atcgtaggga
      361 cagcccttac gactgaagga gtgccgaatg ttcaattcat tcagctttgc ctcataggct
      421 tcgctagtgt actgagaacc caaatctgag tggatgatca agttctcgga gggtgtttgg
      481 tttaagagcg cattgtcaag ggtttcgata accaattctt tcgtcatctg cttgccaaac
      541 ttccaaccga tgactttctt tgtgtggagg tcttgaatag tggagaggta ggtccagcca
      601 tcttttaggg tatgaatata ggtaatatct gtcacccatt tttgattgat cgacgtagtg
      661 gtaaaatctt ggtttaacag gtttttacgt ccttcacttt taactttcgc ttgtttgcca
      721 ggcttgtact tcttgagggt gactgaacgc aagcctaatt ttctcatcag tatttgtact
      781 ttctttaagg acaaggtctc gccacggcca acaagaatct tatgaatttt aggcgcacca
      841 tagataccct tgttttcaaa gtagattcct gaaatcaagc ggtttaagcg tttgttttct
      901 gattggcgct tggaaggttt ctttttcttc cagtcatagt agctggattt cggtgcttcc
      961 aaaacggtac acatttgttt tacgtcatga tcatgaacat ttgattgaat aaattcgaag
     1021 acatcactca tttttacttt tgagcgaata tggttaaagc cttttttagg atagtgtttt
     1081 cctcctgcat gcgggccatt tcctttttca tcgctaggat ttctgcctca gtcatgccgg
     1141 ttgctacatt ctttgtatga agcttcttcc aacgataaat cgtttgtgta gcgataccat
     1201 attgcgctgc cagttcttcg accgattggc cagactggtt taaatctaag atcatcttct
     1261 taaagtcttc atcaaatttt ttcttggctt ttgccatcgt ggacacgtcc tt
//
LOCUS       DACDIH010000193         1304 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.197, whole genome shotgun sequence.
ACCESSION   DACDIH010000193 DACDIH010000000
VERSION     DACDIH010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.197"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..270)
                     /locus_tag="IV281_002869"
     CDS             complement(<1..270)
                     /locus_tag="IV281_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8781791.1"
                     /translation="MSITKNIKCTDEKTSHSIKQILNIKDSHITFSKDAISKERVHGR
                     MANVFTGLLTGEAHRCECCGFDSVIRHAYHDSWIQLLPYQEVPTYL"
     gene            complement(418..976)
                     /locus_tag="IV281_002870"
                     /pseudo
     CDS             complement(418..976)
                     /locus_tag="IV281_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302360.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          391 a          227 c          245 g          441 t
ORIGIN      
        1 tagataagtt ggtacttctt gatacggaag caactgaatc caactatcat gataagcatg
       61 acggatgaca gaatcaaacc cacaacattc gcaacgatgc gcttctccag ttaataagcc
      121 ggtaaataca ttggccatac ggccatgaac gcgctcttta ctaatggcat cttttgaaaa
      181 ggtaatatgg gaatctttga tgttcaaaat ttgtttgatg gaatgacttg tcttttcatc
      241 cgtacatttg atattcttag ttatggacat ttggttatcc tcctatgatt gtttttgccg
      301 atttaattat aggacaacca gatgtctttt tgttataaaa aaatatttgg cgtcactact
      361 cagtttgagt aacaaaacca aatattatag aaccggcttt ttcagttttt tcccttcaca
      421 aacaacatcg atgattttct cggcatgggt acgggtatca ttcattaatt ctgtatcggt
      481 tggatatttg atgttttgtg gagcacaagt ggcatccaaa atcaatgttc cggaattgaa
      541 atcgtcataa gaatcgttgt ccttatcgtc ttttttcttg tcgaagacga gtattttttc
      601 attgatttct atcaacgttg tttcgtccaa gcgtttccgg aaatagacca ttgtagagca
      661 tcgaaaggct tggcatcttg atagcccggc aaaccgatga agaactataa atacgggttt
      721 tcttgaattt gtaagaccgt ttcttcgtcg ctatagctat attccttttg aatcagtaga
      781 gctccgaagg tcatgcgaaa gggttttgcg acgtttcctt ttttttacga aaatttttag
      841 cgtagtcttt ttcaagttca gcccatggaa tgagctctgc tttttgaatc caacggttag
      901 acggattcat tttgagacct atcggttgat tgaaatcttc aaaagccaat tgaacggttg
      961 tttgtaattt atacatgaaa agcctccaaa attgagtggt actccacacc attctgatgg
     1021 tgcataagat aaaaaattaa agaagtgcct gtcatttgtc tgatttaagc caaggtttga
     1081 caaattttgg atattccaat taatactgta agcggttata tttaactcag ggaggtagat
     1141 gaatgtgaat acaaaagatt taaaactgtt agaaaccatt gtacgatcac atgtgacaaa
     1201 ctctcaacaa ttgaaatcat tagtacattt ttcgactagg cagataactt actctataaa
     1261 aaaaattaat gatgaattat cccttcatgg attgagtctg ataa
//
LOCUS       DACDIH010000194         1299 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDIH010000194 DACDIH010000000
VERSION     DACDIH010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.198"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(602..675)
                     /locus_tag="IV281_002871"
     tRNA            complement(602..675)
                     /locus_tag="IV281_002871"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(639..641),aa:Ile,seq:cat)
     gene            complement(865..>1299)
                     /locus_tag="IV281_002872"
     CDS             complement(865..>1299)
                     /locus_tag="IV281_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8781792.1"
                     /translation="TDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGEIENA
                     NLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSFN
                     MRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          408 a          235 c          231 g          425 t
ORIGIN      
        1 gactgacgtg ccggacgcac gtagcgatca tcggcgttat gactatataa agtatacttt
       61 cgttgatcca gaaaggtata aagattaaag taggctacct aaaaaggtag cccggaacga
      121 attttatcac catacttgtg aaaggagata ttttttaagt tagtattaag attgtgtaat
      181 atgatgatat ctatatttta tagtatcagt gctataaaat caaaaataag tcactaatta
      241 actaccactc caattgtaag ccttttttct cacttttttt caaaaatatg gtatgctttt
      301 taatggcttc aaatataaaa gagtttaaag cgtaacacac ttatggggaa gtggtttggg
      361 gtgcgcttta aactcttctt tattattatc tcacaattta acccaaatgt ctttctattt
      421 aaaaatcaaa gtaaaacttt tcaaatatac agaagtataa ctatgtgaaa catcctttca
      481 ttaatccata aaaggataca taaaaaagcc actcatttga gtggcaatga agaaaagctt
      541 tagcttgtat aatactcttc aaaaaaattc taacacagaa cgattcaaat ggctacgtta
      601 atgtaccctg taggactcga acctacgacc ggacggttat gagccgtctg ctctgaccaa
      661 ctgagctaag ggtactggtt gttgccacat aaagccataa acaatcaacc agtagaatgt
      721 gtggcaacaa acctgttatc gcatagcttg gagtgtgact atttatgggt gatagtgaag
      781 atatgcgata acatcactat tttatcgaat aatttttata gttgtcaata tagttatgta
      841 ctgctcctca acgaggagct atttttatcg tttaggaata tttaaatacc aacgtttgtc
      901 atggaaatct tgcgcaccgc ctttagtgtt tccctctgga tcattcgttg cccgcatcat
      961 tacatagact ttcttactag gaaaattacg catattgaaa gatacatgat aaccaacatt
     1021 tccagaagta ttataagctt ggtttacatc tggtctataa attccatcag ctcttactcg
     1081 agctaattct ttcccagtat tgtagtccat aatgaaaata tactcgtatt tatagttagc
     1141 aatgtgccat ccagccacat gcaagtttgc gttttcgatt tccccaaact gatcaatgtg
     1201 ggcgtgattt gttccatctg tcagcgtagg attagctgca ccagctcgtg ttggatcaat
     1261 gacaggcttg ttttcagaag ttgttggatt ttcatcggt
//
LOCUS       DACDIH010000195         1295 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.199, whole genome shotgun sequence.
ACCESSION   DACDIH010000195 DACDIH010000000
VERSION     DACDIH010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.199"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..207)
                     /locus_tag="IV281_002873"
     CDS             complement(<1..207)
                     /locus_tag="IV281_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8781793.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEI"
     gene            complement(189..1163)
                     /locus_tag="IV281_002874"
     CDS             complement(189..1163)
                     /locus_tag="IV281_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8781794.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
BASE COUNT          419 a          245 c          162 g          469 t
ORIGIN      
        1 tatttctttc atactctcct caaaatcatc tggactagta ttttctttaa atggaacagc
       61 actaatacct gtggcgtcat tcattaaaat taattttgtt gcctttaaat aaccctcaca
      121 aagggtagaa tgtgttgtaa tcacaatgtg aaataattga tttgccatct tttgcaacct
      181 cctattaatt aatatttcat ttgccacata tagcgtctaa catctaagga atggcctctt
      241 tcaaaagcaa tcgcttctac catttggcga ataacaacgc cactaagtac agctgcaata
      301 tattctttcg ctacactatc tatagattct aagtcaaaat cagcttggtc taaaaccatt
      361 aatttattac tgaatttttt agtgaagtct tctacccgtt gatctaaaaa tcttgtatct
      421 ccaatacttt tcactaagat gaatggtaca tcataatcag tgatttcaaa aggaccatgg
      481 aaaaattccc cagaatgaat agcattagaa ttaatccatt gcatttccat gaaccagcaa
      541 gctgctgtgg agtaaatttc tccgtaattg ataccgctac caattgtgta aataatttta
      601 tctcgttttt ggtttttacc ccattcttta gcagttattt caaatttgct cttttcagca
      661 ataacaagat catttaactt atcaaattca ttcaagcaag tttcccattt tttagagcca
      721 tctaaaacaa gtaaaatatt aaataataaa ccataaagaa ttcctttatt taaatcacta
      781 gcatcaacat ctttacccca atcatagtga acaggatatt cagctgcttc ccatagagga
      841 gatccttctt cattagataa acaaatagtc attgctccaa attctcttgc tttttttgtt
      901 gcttctacag tttctggtgt tgtgccagaa tgggaacaag tgataactaa agaattttca
      961 gataatcctt ttggtatagc atagttaaat tcgttagcag tataaacatg acaaggtaca
     1021 gttaattctt tatcaaatag atattgacca gccattaaaa cggcttgtga acccccacat
     1081 gctgtaaaat atacatgatc aattgtcttt tcctttaacg ctgccattgc tgtttcaatc
     1141 tgttctttgt gtagttgtct catttcttta tccctcactc taataagtat tataaagtca
     1201 tataacttta caatacctat tatctatgaa attgctttat tttgcaatac tatttttcaa
     1261 aaaaattttt ttataacaat tttatataat aaaat
//
LOCUS       DACDIH010000196         1287 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.200, whole genome shotgun sequence.
ACCESSION   DACDIH010000196 DACDIH010000000
VERSION     DACDIH010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.200"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            89..421
                     /locus_tag="IV281_002875"
     CDS             89..421
                     /locus_tag="IV281_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP8781795.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1040..>1287
                     /locus_tag="IV281_002876"
     CDS             1040..>1287
                     /locus_tag="IV281_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP8781796.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNG"
BASE COUNT          462 a          173 c          282 g          370 t
ORIGIN      
        1 tgtcatgaac gaaaatcaat tctctatttt tgaaaaataa caagaaaaac ttgttaaaaa
       61 tatttaaaga aattgggtgg tttattttat gtcatataca ggaattttga gtctcaaaga
      121 tatttgtcac tacggtaaac ggtgtaccgc tacagagaaa atcacgaaga aactatcaac
      181 tggccaaagt aaaaccgttg tgcagtgtaa gaaatatata atccagaaag acaaagtctc
      241 agaggaaatg atttattaca ttgggaaaca aaagcaaata attttaaaag atcctatccc
      301 cttaaaagag ctatatccaa caatcaaaca tgtttatgac caaaacggtg tactgattgg
      361 tcgaagaaaa aatggagtct tgcgttgtac cgcaaaaggg atggggcgtt tgatcggctg
      421 aattgatcat tctcataact tgtattttat cgtttttcgc ttgcttgaaa atattgtcga
      481 gacttctaaa ggaacttaga gtcactagaa aaataaggag tagacttgat ctatcaacaa
      541 ttcccttcgg ggagtgacgg ggtaggtttt aacgattggt aacgtaaaaa attggtcaat
      601 cgagtaaatg cgcttgtttg gatagtggtg aaatgaattt gtgtaaataa taagcgcaga
      661 gtaaacaaga gggaatttag aagttagaca agttagaata gcgtctagat taatcatgag
      721 cagctactaa gggatctaga ctttgacgca aactcatgga ataaggagta ggtcttctcg
      781 taatggggag gacgcacaag aaattgtgtc gtgtgctgca gtacatgtag atacttgcga
      841 ggacgttgat tattggatca atcgagcatt taccgctgtc tactagggtt tctcgtttcg
      901 gacgccataa acgagtcgaa attttttatg tatttttaag taaacgaaaa acgataaaat
      961 atgaacaagt cctgaataaa gtgaaaaaat taatataaag catagagaaa atgaaattta
     1021 cgtaatggag gaatgacaaa tgaaaaatta tgatccgaat atacgattgg gcacacatac
     1081 tattaaggtt tctttccaac gttgggatta caaaggattt gtgacgttta gaaggggcgg
     1141 aaattgtaaa ggattagatg ttttagcatt agacgaggat gatttatacg atcaaacatt
     1201 gactgataat ccaattggat tcggcttgct acctgaagat gatgaaggta atgaatggtt
     1261 taaaatgacg cttatgaatg ataatgg
//
LOCUS       DACDIH010000197         1265 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDIH010000197 DACDIH010000000
VERSION     DACDIH010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.201"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(62..280)
                     /locus_tag="IV281_002877"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="IV281_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="IV281_002878"
     CDS             complement(546..740)
                     /locus_tag="IV281_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781797.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          448 a          213 c          173 g          431 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gtttt
//
LOCUS       DACDIH010000198         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.202, whole genome shotgun sequence.
ACCESSION   DACDIH010000198 DACDIH010000000
VERSION     DACDIH010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.202"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            18..1148
                     /locus_tag="IV281_002879"
     CDS             18..1148
                     /locus_tag="IV281_002879"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP8781798.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
BASE COUNT          448 a          205 c          246 g          347 t
ORIGIN      
        1 agaaaagagt gagtgaaatg agcaaaaata gaccatttac agtagatcat gtcggaagtt
       61 tcttacgccc gcaatcgatc aaagaagcaa gaaaaaaagt tcaagaaggc ttactttcaa
      121 aagaagaact tcgtgcaatc gaagaccagg aaataaaaaa attagtggaa gaccaaaaag
      181 cagtcggcat caaaggaatt acagatggtg aatttcggcg tggattttgg catcttgatt
      241 ttttagaaga actaaatggt atcgaaggct atgttccaga aactggatat aatcaaacat
      301 tccacggaaa agcagcacca gcttataata ttcgggtagt agataaatta tctttcaatc
      361 caaatcaccc atttttaaaa gattttgctt ttctgaaaga agtagtggaa acagatgata
      421 cttttgttgc aaaagcaacg atccctagtc ccacgatgat tttacgacaa gagatcctat
      481 ctaatgatgg ttcttcaaaa atcaaggaga tttatcctga tctatctgat ttttatgagg
      541 acttgtcctc tacttacaaa gcagcgattc atgcttttta tgaaaaaggt tgccgttacc
      601 tgcaattcga tgatacaaat tgggcgtttc tagctgatca aactaaaaga gaagcacttc
      661 ttcaaaaagg aattgatccg aaagaaatgg cccatatttg tacagagatt atcaataacg
      721 cactggaaga caaaccagaa gatttgacga tcacaacaca tatctgccgc gggaatcacg
      781 cttcttcttg gctgttttcc ggtggatatg aacccattgc agaagagttg ttttcaacaa
      841 attatgatgg tttctttttg gaatacgatt ctgaccgagc aggagatttt actcctttaa
      901 gacattggag taacaaagaa agtaaagttg ttttaggatt agttacttct aagtttccag
      961 atttggaaga tacggagaaa ataaaagaac gaataaaaga agcacaacaa tttgtaccat
     1021 tggaaaatct aagtatcagt cctcaatgtg ggtttgcttc aacagaagaa ggaaaccatt
     1081 taacagaaga agaacaatgg aagaaaatcc aattattgca gcaagtcgca aaagaagttt
     1141 ggctataaaa atgtgaggct tggcaaaaaa aagcgtccca aaaagtcggc ttttcatgga
     1201 gtggagaaat ccactctttt ttatttacct tttatattat ttattt
//
LOCUS       DACDIH010000199         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDIH010000199 DACDIH010000000
VERSION     DACDIH010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.203"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            200..742
                     /locus_tag="IV281_002880"
     CDS             200..742
                     /locus_tag="IV281_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781799.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            832..>1246
                     /locus_tag="IV281_002881"
     CDS             832..>1246
                     /locus_tag="IV281_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP8781800.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DACDIH010000200         1239 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.205, whole genome shotgun sequence.
ACCESSION   DACDIH010000200 DACDIH010000000
VERSION     DACDIH010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.205"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(102..437)
                     /locus_tag="IV281_002882"
                     /pseudo
     CDS             complement(102..437)
                     /locus_tag="IV281_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            619..1224
                     /locus_tag="IV281_002883"
     CDS             619..1224
                     /locus_tag="IV281_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8781801.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKRANDL"
BASE COUNT          446 a          198 c          201 g          394 t
ORIGIN      
        1 tgatttatta gtattcttgt gttttcaatt ttatacattc aaaaatagta tctggaacat
       61 ccgtgcgaaa atgcacggta ggaaccaaaa gacgaacatt ctcaataatt ctctatattt
      121 aacggacgta gtattccatt cgataattct ttttcatagc ggaaactata tttgccaaca
      181 aaattaatgt gatcactaat taatggaaat tgcttgcgta acacttcttc ggaacagtcg
      241 aacccttccg ttttaatttg ttcaaagact ttttctaaat atagtgaatt ccaatatatg
      301 atagcattag ttactaatcc taatgcgttg agctgttcct ccataccgtc gtaataagtt
      361 tgataaagtt tgccttttct accataaaat atatttcgac atagagcatg acgagcttca
      421 cctttattta actgttccga gctaggctct cctaaataaa caccaaaaga atttttattg
      481 cgcttaattt cattcattca aaacctccaa agtaaattat aagtagactt aaaatttatt
      541 tttaaaaatg ctatgataac agtaaaacta caagtaaatt ttacatgtaa attatatcat
      601 tttttaaaag gagtttttat ggcaaaaatt ggatatatta gagtgtcttc acaagatcaa
      661 aatttagatc gacaattggc aatgatggaa gaccttaaca ttgataaatt attccaagaa
      721 aaagccagtg gtaaagatat ccagcgacct gagtttcaaa aattactaaa ttatatccga
      781 gaaggtgatt gtgtcattgt tgcatcactg gatcgcctag gcagagatta tgaagacata
      841 aaaaatacag ttacctttat gaaacagaaa aaagtagcat taaaaatact agatgcacag
      901 tttttagatt ttaatactgg aaatgaactc ctagatactg ctatgttcga tatgttttta
      961 tcattgctaa gttatattgc acaaaacgaa cgtgagaaaa tccgtgaacg tcagcggcaa
     1021 ggagtattat tggccaaaaa agctggtcga tataaggggc gaccaattga atattctgag
     1081 aactcaacag atccgcaaaa acgattagtc tttaaaactg tggtggacat gttaaatcaa
     1141 ggagttccag ttgcagaaat tgccaaagaa aatggtctct ctcgaccaac catatataaa
     1201 ataaagagag cgaatgattt gtagaattta tactatatt
//
LOCUS       DACDIH010000201         1235 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDIH010000201 DACDIH010000000
VERSION     DACDIH010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.206"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            35..532
                     /locus_tag="IV281_002884"
     CDS             35..532
                     /locus_tag="IV281_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8781802.1"
                     /translation="MNNQIALAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(569..1186)
                     /locus_tag="IV281_002885"
     CDS             complement(569..1186)
                     /locus_tag="IV281_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781803.1"
                     /translation="MYSQTIDSLNKRFGNRVLKGIEIGYIASEKDRIIDYLADKDYDL
                     KLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSEALVVIEADVFAHFDYGFRVFGL
                     SVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLYDNLALYEYVIDLYLSRGGTLFS
                     VGSDGHYLEHFRFHFDDLFALLKAKGVTELAIYQKRKRIMVPLPV"
BASE COUNT          431 a          208 c          216 g          380 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatcg cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt agactggtaa tggaaccata attcgctttc
      601 ttttttgata aatcgctaat tccgtcacac cttttgcttt taacagagca aaaagatcat
      661 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      721 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      781 aagcgctctt agcatttaat tcgaatgcca atttattttt gattacttga tctagaatcg
      841 gtaaaaactg cgcttcatat tgtttaaatt cagcaacgga tagaccaaat acccgaaaac
      901 catagtcaaa atgagcaaag acatctgctt ctataacaac tagtgcttct gaaagttgtg
      961 caaaatactg aggaatcaca atagcaggat ccatatcttt gacttcgtca tctaaataat
     1021 caaattgacc attatgatgc acactgagta attttaaatc gtaatcttta tcggctaaat
     1081 agtcgatgat ccgatccttt tcagaagcga tgtaaccgat ttctatgcct tttaaaacac
     1141 gattgccaaa acgcttattt aagctatcga ttgtttgact gtacatttca taatcgggga
     1201 tatcatcgac tttagtaaca ggattggcta agtca
//
LOCUS       DACDIH010000202         1216 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDIH010000202 DACDIH010000000
VERSION     DACDIH010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.207"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..387)
                     /locus_tag="IV281_002886"
     CDS             complement(<1..387)
                     /locus_tag="IV281_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP8781804.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYI"
     gene            complement(546..812)
                     /locus_tag="IV281_002887"
     CDS             complement(546..812)
                     /locus_tag="IV281_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8781805.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(802..1158)
                     /locus_tag="IV281_002888"
     CDS             complement(802..1158)
                     /locus_tag="IV281_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8781806.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          334 a          240 c          152 g          490 t
ORIGIN      
        1 tatatactct tcagccaaaa cttgtggaac tggttcatct aagaaaaaaa cccctacctc
       61 gcctttgcta tttttataaa agtaatcaat ttctgctata gttcccgatc tataattatc
      121 aactggagaa cccaacttat ggccaaaaat cgctaataat atatcactat tttctacaat
      181 ttgtttatta ataacacttt gcccagagct attatttata taagtaggaa tcgcattcgt
      241 ttcccatcga attggcatca aaattacttt tgtattatct gtattttcta tattccatct
      301 atatatggtt tcttctactt cattcctctt tgctgctata tcacctggtg atgctataaa
      361 tacttttatt agatttatgg ttttcattta ttgtaccttt ctctttatgt ctctttaaag
      421 taatttttgt ataaaattta ttgactttta cactatagga caacttttat ctatttagta
      481 caaaaaggcc cactaaacta attgattagt taggagccta ttacaggtat attttcaact
      541 cgcttttaag caaattctac taccagcttt ttgcctaatg ccctagcgat atcatcaagt
      601 ttatcgaaag atacgttatc ccctctctca attcgagcaa tagtagattg agttgtatgt
      661 gctttttgtg caagctccgc ttgagttagg cctgcttcct cccgtgcatg ataaagagca
      721 accgcagttt cgagttttct tccttcacgg tcatatgctt ctgcaaaatc tttgtctttt
      781 tgcttttctt caatcaattt tttaatcatc atttgtgaac cactcctctc taagttcttt
      841 tgcatgttta agttcactta ttggtgtttt ctccgttttt ttagtaaatc catgggtaat
      901 gacatacctt ccattgacca catggaaata gatagctctt tggatgtttg atcctacctt
      961 acttcttaat tcgtacaaat tagtgtctaa ttttttcacc catttcaacc gttgagctac
     1021 tagcatccca ttcttttctg tttcttctat tgtacgtaaa agcttcgcac tatctttttt
     1081 tggtaatgtt tccaaccatt ctaaaaattc atcatgacca ttaggtcttt taaatgtttc
     1141 aaaagatatt ttttccatac ccaattatat catatattga ttttatatta tagtatataa
     1201 cataaaaatt cgaatt
//
LOCUS       DACDIH010000203         1188 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.208, whole genome shotgun sequence.
ACCESSION   DACDIH010000203 DACDIH010000000
VERSION     DACDIH010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.208"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            183..647
                     /locus_tag="IV281_002889"
     CDS             183..647
                     /locus_tag="IV281_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8781807.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            661..822
                     /locus_tag="IV281_002890"
                     /pseudo
     CDS             661..822
                     /locus_tag="IV281_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019893466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(816..1188)
                     /locus_tag="IV281_002891"
                     /pseudo
     CDS             complement(816..1188)
                     /locus_tag="IV281_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019893466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
BASE COUNT          422 a          172 c          219 g          375 t
ORIGIN      
        1 acctctttta ctggagttat taatggccca gtcagctggt aaatctatag aagaaataga
       61 ttttaaggaa ttactgaata actctcaaaa aagtatggtt catattaatt cgcttttaaa
      121 agatgtcagt taaggtggca atttctattc tataaatttt aaaaattgaa ggaggaaata
      181 gaatgattaa attagtgcgt gtagaccaca gattacttca tggacaagta atattttctt
      241 ggactaaaca gtatgatatt aaccatatta ttgtggctga taacaaagtt ccaggagatc
      301 caatgtctgt gatggctttg acaattgcta aacctgctga ttgcaaatta gacattgtta
      361 aaattagtga tgttaaaggg attatggaaa catccccaaa tgataattac atgattttaa
      421 ttaaaggacc taaggaagct tttgaattag ttcaaaattt accagaaatt aaagaaatta
      481 attttggcgg tgtgtctaag aaaagcgagt ccaaacaata tggaaaggca gtctttttaa
      541 ctcctgaaga attagaagcc acaaaaaaaa ttcttgatca agaagtgaaa atatttgtgc
      601 aacaagtccc atcttcaagt attgaaaatg tagattttag taagtaaggg gggaaaataa
      661 atgttattaa aagccatttt attaggttta gttggtgtgg tagctgttat tgactccagg
      721 ttaattggtc gacaaaatat tggtagacca ctaattttga gtacacttgc cggacttgtg
      781 ctaggtgact tgcatacagg gattgttctt ggggcgtctg taatcatttc tggaatttta
      841 tctactaaat ctgccacaac gtcagcacca aaatatactg ttaaaaagat tggaataaag
      901 taaacaaaag cattaaaaat aaatgaccaa taaatatgaa taatttgagc ttttctaaat
      961 ttattatttt caatggcttt atctgcagca tgtccaaatt gagcaattat catgcgcatg
     1021 ataatactca taaattcacc agctacagcc actgggatag ctattgttaa ggctgtttct
     1081 gtactactat tagttaaaat tgcaaaagca gtggcaataa tactaccaag ttgcatatta
     1141 ggaggtccag ccgccccaat tgagacgaat cccatagaaa ttaattct
//
LOCUS       DACDIH010000204         1172 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDIH010000204 DACDIH010000000
VERSION     DACDIH010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.209"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..701
                     /locus_tag="IV281_002892"
     CDS             <1..701
                     /locus_tag="IV281_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP8781808.1"
                     /translation="KELQREIMPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITK
                     NFPSNIPNNYLEYYLYPDMVVEHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVL
                     NFNFGEHGQYIVDIAISLLNDDRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEP
                     ITLRKPISDFHKGLMEAQVAVEKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILD
                     EMIKVNKKYWPALK"
     gene            714..1172
                     /locus_tag="IV281_002893"
     CDS             714..1172
                     /locus_tag="IV281_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8781809.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
BASE COUNT          438 a          173 c          242 g          319 t
ORIGIN      
        1 ataaagaatt gcaacgagaa attatgccag agattattga aaaactaata acaaaagaaa
       61 tgcaagtcgc cgattttaat ataggagata aaacttggca aaaaaccttt caaatgatgt
      121 cagtcatcac gaaaaatttt ccaagtaata tcccgaataa ttacttagag tattacttat
      181 atcctgatat ggtcgttgag catactgaca aagagtatac acgtgctaat atggtcatgg
      241 atggacgaga gaaaaatacc aaagaaatgg ctgataaaat acgccgcggt attgtggaag
      301 aagtattgaa ctttaatttt ggggagcatg gacaatatat cgtcgatata gctatttcct
      361 tgttgaatga tgatcgtcgt cgctttatgc taatcgttcc taatcaaggg gcgattccga
      421 atctacggtc agatgcagtt gttgaaatac cggcatatgt tggtgcaact ggtgtggagc
      481 caattacact aagaaaacca atcagtgatt ttcataaagg attgatggag gcacaagttg
      541 cagtagagaa gttactagta gatgcttact ttgaaggttc ttatcaaaaa gctttacaag
      601 catttacatt gaatcaaacc gtaccaaatg ccagagtcgc aaaaaaaata ctagatgaaa
      661 tgatcaaagt aaacaaaaaa tattggccag cattaaagta gaggtgataa aaagtgcatt
      721 tctttaaaac aagaaacatt ctaaaagctg tagctagtgg tcaaattatt ccattaacag
      781 cagtaaaaga cgaggtcttt tcttcaaaaa tgataggagt tggatacgct ataacgaatc
      841 atgatggata tatccatgcg ccagttaaag gaataatcga taatatattt cctacccgac
      901 atgccataac gatcgaaagt gaatctggca gaaaaatact gatccatatg ggtttagata
      961 cagttgaact cgaaggaagt ccgtttcaga tacgggttga aagtaaccag gaggtaaaag
     1021 aagggacact gttagctatt atagatttga agaagttgga gcaagcacaa aaagatacgg
     1081 tgatcattgt agtttctcca ggcatttttg aaggaaattt attaaaacaa aaccaagaag
     1141 taaagattaa agatagttgt tttgaatttt ag
//
LOCUS       DACDIH010000205         1170 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.211, whole genome shotgun sequence.
ACCESSION   DACDIH010000205 DACDIH010000000
VERSION     DACDIH010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.211"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            44..667
                     /locus_tag="IV281_002894"
     CDS             44..667
                     /locus_tag="IV281_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8781810.1"
                     /translation="MKIAYIRVSSIDQNEQRQIEEMKKFGAEKIFIEKQSGATITHRP
                     IFQEALEFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PHLDKFIKDLIIQILAMIAEQERTESKRRQAQVIKIAKANGVYKGRPKLYSADTKNPQ
                     RRLVYENIVKDLNEGIAISKIAKNYNITRQTIYRIKNDIEYNSNQMN"
     gene            complement(664..>1170)
                     /locus_tag="IV281_002895"
     CDS             complement(664..>1170)
                     /locus_tag="IV281_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781811.1"
                     /translation="NEDNSQKQIIIFELYLKWLSSLSVSPESNDDDKYFANKLFQFIF
                     PDYSSMPFFTLINFMLYVKEHLGYDDLVEMIKAYDKEPYPFKGINTGSDMAMISGETV
                     EEILANYKEQNIKTLELIKKNIKYYDPLLANKENIDKLLNAIADSDVRSPRIDELKEE
                     ILMYKELF"
BASE COUNT          416 a          186 c          180 g          388 t
ORIGIN      
        1 ttttgagtgt attttaaata gtttgaggaa gtaggtaaaa attttgaaaa ttgcttatat
       61 tcgagtttcg tcgattgatc aaaatgagca acgccaaatt gaagaaatga aaaagttcgg
      121 cgcagaaaaa attttcatcg agaaacaatc tggagcaacg attactcatc gaccgatttt
      181 ccaggaagct ttagagtttg tccgagacca agatattttt attgtcgaag caatcgaccg
      241 acttggaaga aattacgacg aaatcattgc ttcagtcaat tacctaaaga agaaaaacgt
      301 gcagctgatc atcaccagtc taccgatcat ggctgaagca attggcaatc ctcatttgga
      361 taaattcatt aaagatttaa tcatccaaat tttggccatg attgcagagc aagagcgtac
      421 cgaatctaaa cgacgccagg cgcaagtcat taaaattgca aaagcaaatg gcgtctataa
      481 aggtcgtcca aaactctata gtgcagatac aaaaaatcct caacgacgtt tagtttatga
      541 aaacatcgtt aaggatttaa atgagggcat agctatttca aaaattgcta aaaactacaa
      601 tattactaga caaactattt acagaattaa gaatgatatt gagtataaca gtaatcaaat
      661 gaattaaaaa agttctttat acattaaaat ttcttctttt aactcatcaa ttctaggaga
      721 tctaacatca gaatcagcaa ttgcatttaa taacttatca atattttctt tattcgctag
      781 taaaggatca tagtatttta tattcttttt aattaattcc agagttttta tattttgttc
      841 tttgtaatta gctagaattt cttctacagt ttcgcctgaa atcatagcca tatctgaacc
      901 agtattgatt cctttgaatg gataaggttc tttatcatat gcctttatca tttctactaa
      961 atcatcatat cctaagtgtt ctttaacata cagcataaaa tttattaatg taaaaaatgg
     1021 cattgaagaa tagtctggaa atataaattg aaacagtttg ttcgcaaaat atttatcatc
     1081 atcattgctt tctggtgaca ctgacaggct gctaagccac tttaaataca attcaaatat
     1141 aattatttgt ttttgtgaat tatcttcatt
//
LOCUS       DACDIH010000206         1144 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.213, whole genome shotgun sequence.
ACCESSION   DACDIH010000206 DACDIH010000000
VERSION     DACDIH010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.213"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            544..930
                     /locus_tag="IV281_002896"
     CDS             544..930
                     /locus_tag="IV281_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8781812.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1028..>1144
                     /locus_tag="IV281_002897"
     CDS             1028..>1144
                     /locus_tag="IV281_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8781813.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGK"
BASE COUNT          430 a          174 c          168 g          372 t
ORIGIN      
        1 atcaccgaga actcatcatt tttcatcatg atgaatacta tttacatata tctagttcaa
       61 agacaataat tattatgatc cgttattatc taagcactat acagataata ggaatttttc
      121 gtttctaaat actccaattt gggaatccaa taaaaatttt cgttcaaata cgtgtctatt
      181 cgaactcttt tctagttgtt aattatgaag ggggaagaaa taaaaaatac aatctataca
      241 cgaataagaa tattatccaa aaggaggttt tagaaaagat ttattatacg taaaaagctc
      301 aagacatttt cttagccata aggacatgtt tgatatttaa gaagatatat gaattttctt
      361 ttctaagtat aaagataact atccattatc aactaaaaaa acatttttga aaataaacat
      421 cagcagtcct atcaatgcat gtgagcttat agaagttatt cgcagcgcag cataattgca
      481 ttagcttctt ttcgtgtttt ttgcctcttt tccctttaaa tcttaatgga ggaggtgtgc
      541 aatatgtcag ataacgacga acgcgtaaat gcccagttga ttcgattctt tgaaaaggtc
      601 attttgaaca ccgctaatac ttattataaa aaacaagaag aaattcaaaa acatgaacaa
      661 cttgatcaac tcccaccata tttgtttact aaagatacta taaaatttaa acaaccgatt
      721 aatgttttaa atgtgactct ccttgttgaa gatataaaat tagcaaaaat ccttcctttg
      781 ttgaaagaac ctgaacaaat ttttcttatc gaaaaattta tctttaataa aactgataaa
      841 gaaattggaa aggttctggg gattacaaga cagggtgcga caaatttaaa acatcgactt
      901 tacaaaaaaa ttcaaaaaat gctggtttaa tatctgcata ataactaatt ttgtatgctt
      961 ctttgctcgt cctcctcctt cccaaaaaaa taaatagatt ataagttcat gggaagaagg
     1021 taaatttatg aatactttaa aagaagattt cattttagca aagaatggaa atgaagaagc
     1081 agttgtggcc attctcaaaa gatttagcgc tttggtccat aagcaatcct ggagaactgg
     1141 aaaa
//
LOCUS       DACDIH010000207         1089 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.214, whole genome shotgun sequence.
ACCESSION   DACDIH010000207 DACDIH010000000
VERSION     DACDIH010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.214"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            60..917
                     /locus_tag="IV281_002898"
     CDS             60..917
                     /locus_tag="IV281_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8781814.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
BASE COUNT          412 a          157 c          232 g          288 t
ORIGIN      
        1 aaaataaata tgttataata gtatcaaatg aatattgcta aaagaaaaga ggtaggaacg
       61 tggtagcgaa gaaatatgta atcggtaatt ttaaaggtgg cgtaggtaaa agtacgtgcg
      121 cgcaaatgtt agggtttgaa tcagcagtta atgaaggaaa aagaaccttg ataattgact
      181 tggatatgca aggaaatact tctgatgtga tgaacttaac gcatatgaac ttcactaacg
      241 aagaaggagg cggagaaggt gaaccgattg tttacgaaaa tactgtgact gacgtgttga
      301 taaacaatct tccacctaaa gaggctgtct ataaagtaat caataacctc tatatacttc
      361 cagcagacat gtcctttgaa atgtatgatg attggatcaa agaccaatat aatgattcat
      421 tatcccaatt caaatatatg aaagaaaagc tagaaccatt atttgatgag tttgacgtca
      481 tctatttaga tgtcccacct tctatttctg tatatagtaa atccgcgatg tacattgcgg
      541 attgggcaat cgtcgtctta cagacccaag taaaatcgat gagaaatgcg atgcaatatc
      601 ttgagtatat ggactttttt gtgaaagaat ttgatacaaa tttatatgtt gctggtgtta
      661 ttcctttcat gttggaaagc ggtgacgcag tagatcagga aatgtataaa caagcccaag
      721 agatttatgg ggaacatttg attaaaaatg ttgtactcaa aaatgcgcgc ttaaaacgat
      781 acgacggatc aggtattacg atggagaaga caaagaaagg tctcttaaaa caatgggatc
      841 gcaaaagcca cgaattattt atcagtattt tgaaagaatt ggaagaacac gagaattggt
      901 atgcggaggt ggagtaattg gctgacatca aaggacttga tatttttggg aacaacaaag
      961 gactcgccaa aataaaaaaa gaagcagaag caaagaaaaa gcagcaagca attacaccat
     1021 ctgaaaataa gcaattagaa gtgcaccaag ttcaagaaat aaaaacttcc tccaatgttg
     1081 aaagacaga
//
LOCUS       DACDIH010000208         1080 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDIH010000208 DACDIH010000000
VERSION     DACDIH010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.215"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..968
                     /locus_tag="IV281_002899"
     CDS             <1..968
                     /locus_tag="IV281_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP8781815.1"
                     /translation="SITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVNDLDT
                     HYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFIPWI
                     LGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHIYDN
                     PVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSSV
                     SRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCNV
                     DKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          379 a          135 c          185 g          381 t
ORIGIN      
        1 cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat tatgaaatat
       61 cttacaatga agtagttact gttactccaa gaaattataa gttgaatttt cgaaaggtaa
      121 atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct gactaccctc
      181 tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat ttttctgggt
      241 atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt gaatttattc
      301 cttggatttt aggacaatgc tcaacagtag gagaaactaa aaaattgtta aaaaatatca
      361 atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat tggctattag
      421 ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat atatatgata
      481 accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt aatttaaaca
      541 attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa ataagtttga
      601 atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc tcagtatctc
      661 gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca gagtcagaaa
      721 gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt ttgtgtgatg
      781 ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt gacaaaggaa
      841 tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg aataaagaag
      901 acttagatag tcataagtta attagttatc caattataga aaaacaacaa attaaatata
      961 taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa aaaggcaaag
     1021 atattttcaa aacatttttt cttgatgtga tatccttgtc ttttttgtaa tgtttttaac
//
LOCUS       DACDIH010000209         1076 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.216, whole genome shotgun sequence.
ACCESSION   DACDIH010000209 DACDIH010000000
VERSION     DACDIH010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.216"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(368..>1076)
                     /locus_tag="IV281_002900"
     CDS             complement(368..>1076)
                     /locus_tag="IV281_002900"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8781816.1"
                     /translation="TAGINQKPGQSRLDLVKTNASIMRQIVKEIMGSGFDGIIVVASN
                     PVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIALKLKVDPRSVHGYILGEHGDS
                     EVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKVRNAAYEIIDRKKATYYGIGMS
                     TARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPSIVDENGVREVVELSINEEEKA
                     MFSKSTSALREVLNTVL"
BASE COUNT          321 a          197 c          172 g          386 t
ORIGIN      
        1 aaaatctgct tatgcaaaat tataaaaaaa ttttgcagtt tttcttactt tttttgtcgt
       61 ttaattgaat gataaaaacc agtttcttta ttcagttaat caaatatgaa cgaattagat
      121 atgaatcatt gtatttgccg ttgcggaagg attttttaat taaccacaga taaacattga
      181 taacgattcg cttattgtaa tcaacagatt aaatttcata tgttcatatg tacggataca
      241 ttttcggacc gattaatttt aaccattctg tttatttgct tacctattag atatcaataa
      301 aaaactgctt aacagctatt tcagctgtta agcagtttta ggatttttag ttcatattca
      361 ttgtttctta taatacagtg ttcaatactt ctcttaaagc acttgttgat ttgctgaaca
      421 ttgctttttc ttcttcgttg attgataatt caacaacttc acgaacacca ttttcgtcaa
      481 cgattgatgg cacacctgta aagatatctt tttcgtcgta ttcaccagtt aaataagcag
      541 atacaggcaa tacagcttgt tcgttattca agatagcttt tacgatacgt gcagtactca
      601 taccaatacc atagtaagtt gcttttttac gatcaatgat ctcataagca gcattacgga
      661 ctttatcagc aatcacatct aattcatctt gggcaatacg atggtctttt tctacaattt
      721 caaacactgg tttaccaccg acagttgtat gtgaccatgc agcaacttct gaatcaccat
      781 gttcacctaa gatatagccg tggacgctgc gtgggtcaac ttttaatttc aatgcgattt
      841 ctttacggaa acgagttgta tccaatgttg ttccagtacc gataacacgt gatgttggca
      901 gaccagattc gttccaagcg atatatgtca aaatatctac tgggtttgaa gcaacaacga
      961 taataccgtc gaatcctgat cccatgattt cttttacgat ttggcgcatg attgacgcat
     1021 ttgttttaac taaatctaaa cgagattgac caggtttttg gttgatacca gctgtg
//
LOCUS       DACDIH010000210         1066 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.217, whole genome shotgun sequence.
ACCESSION   DACDIH010000210 DACDIH010000000
VERSION     DACDIH010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.217"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            64..879
                     /gene="thiD"
                     /locus_tag="IV281_002901"
     CDS             64..879
                     /gene="thiD"
                     /locus_tag="IV281_002901"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP8781817.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          378 a          182 c          239 g          267 t
ORIGIN      
        1 atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg aggaatcgat
       61 ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag cgatggaagc
      121 gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg tgtttctgtc
      181 atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac attgccaaca
      241 gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg tgcagctaaa
      301 acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta ccaaaaatat
      361 gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa tcttttacta
      421 gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga agtactgaca
      481 cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga agaagatttt
      541 gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat caaaggacat
      601 catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga atcaggaaaa
      661 tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac tggcgattcg
      721 ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca agcgatccga
      781 ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg gcacaaatat
      841 ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt tgtgaaaaag
      901 ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg tttttaactt
      961 ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag aggtcgtgag
     1021 cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagc
//
LOCUS       DACDIH010000211         1062 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDIH010000211 DACDIH010000000
VERSION     DACDIH010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.218"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(37..993)
                     /locus_tag="IV281_002902"
     CDS             complement(37..993)
                     /locus_tag="IV281_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781818.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DACDIH010000212         1057 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDIH010000212 DACDIH010000000
VERSION     DACDIH010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.219"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..545)
                     /locus_tag="IV281_002903"
     CDS             complement(<1..545)
                     /locus_tag="IV281_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781819.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKT"
BASE COUNT          318 a          212 c          137 g          390 t
ORIGIN      
        1 gttttagggt tatcttcatc ctcacgtgca aaactaaaag cgtactctag ttttggttca
       61 tcgccaactt ccctagacat aaatgaacat ctcctgacgt ggtttaaaag taccgcatct
      121 tcgacgtctg tatacgatgc acagcacaga ttgcttcttt tgatctttct tttgattttt
      181 ctgttgcttc ttctacacct tcttcgatta ctacatcgat gcttacaatt gcttctgcct
      241 aatattccca tgcatcgtcg atttaagcct ctgtagtcac tgtgatcgtt tccacatttt
      301 aactcctctg tcgtggcttc agttgatttt gtgatcagtt cagttgctaa gccagagtca
      361 gaacaacctg ccattgctag cgttaatatt gatagtaaaa atagtttttt tatcataagg
      421 ttccccccct gaaaatagac taaaataata acgctttctt tttttgtttt cagacgaaaa
      481 ttaataattt ctaaatattc tttaaaaaag cttataacta ttatgccttt tttaatatat
      541 aacacagtta tttttaaaaa taagaatgct aaatcactct acaataacgg cgaaagcaca
      601 aaaaataaaa aaattattac gttattaaaa aaacagatga aagatcaggt tttattagat
      661 ctcattcttt catcacttga agggatttaa tgaactactt tttcctcttt gtcgttttta
      721 tatattcgta ttccttcaaa cagtaggata gcctctttca atgcttgata tttgaactgt
      781 ccgttactta aaaatttaga tgtgccgccc tcttcctaga acatattctt ttttactttt
      841 tcattaattg acagaccaat cgaacgaaac tgtaaatttc gataggataa tgattggtca
      901 aactcattca ttttcaagac atctcgattc atattcaaga taggaatctt tttatcaaca
      961 tcactttatt cgtattttcc tctaccataa aagcttcacg ctccacaaaa ttgttttata
     1021 cactactatt cagcccattc aacgaccaat gaatttt
//
LOCUS       DACDIH010000213         1056 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.220, whole genome shotgun sequence.
ACCESSION   DACDIH010000213 DACDIH010000000
VERSION     DACDIH010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.220"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..1056
                     /locus_tag="IV281_002904"
                     /pseudo
     CDS             1..1056
                     /locus_tag="IV281_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT          333 a          208 c          205 g          310 t
ORIGIN      
        1 gtagaaatca atttgcgatg cttacgattg atgatctagt tcctcaagat catctcgttc
       61 gaaagattga tgccgcgctt gattttgaat ttatttatcc aatcgtcgaa gccacttact
      121 ccgatttagg tcgcccaagt atcgacccag tgattctgat caaattggtg tttattcagt
      181 atttattcgg tatccgttcc atgcgacaaa ccattaagga agtcgatacc aatgtcgctt
      241 accgctggtt tttaggctac tccttcgaag agaaaatccc ccatttctca acttttggaa
      301 agaattatgt tcgccgattt agagaaacga cggtgtttga ggatattttt gcctatattt
      361 tagaacaagc agttaaagca ggttttgtaa cagaggataa ttttcatcta gattctaccc
      421 acatcaaagc taacgccaat aaacataagt ttacgaaaga aatgacccat gatgaagcga
      481 aagcctatca ggatgaatta gaagatgaga tcaatcgtca acgaatagag gctggaaaac
      541 gcccttttac tttggactta gaaaaggaag tgaagctgaa agaaagaaaa ataagtaaag
      601 ctgatccgga aagcggctac tacgtcaaag gcgaacaaga aaaacaattt gcttattctg
      661 cccatacttc atgcgacgac aatggattta ttcttagcac aatcatcact cctggaaata
      721 ttcatgacag ttaagtcgcc tttcagctcg tcaaacaatc gaagagactt tttcctgaaa
      781 tcaattgtgt tgtcgctgat gctgggtata agacacctaa gtttgttcac tttttgactc
      841 atttgaacct tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gggctattat
      901 caaaaaatga gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa
      961 tgctcgcttt ttcaacggtt actcgagaag ggtaccgaga atacaaatcc aatccaaaag
     1021 aatgtgtgaa ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DACDIH010000214         1038 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDIH010000214 DACDIH010000000
VERSION     DACDIH010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.221"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>1038
                     /locus_tag="IV281_002905"
     CDS             <1..>1038
                     /locus_tag="IV281_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8781820.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKH"
BASE COUNT          361 a          204 c          187 g          286 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacacc
//
LOCUS       DACDIH010000215         1035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.222, whole genome shotgun sequence.
ACCESSION   DACDIH010000215 DACDIH010000000
VERSION     DACDIH010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.222"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1035)
                     /locus_tag="IV281_002906"
     rRNA            complement(<1..>1035)
                     /locus_tag="IV281_002906"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACDIH010000216          994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDIH010000216 DACDIH010000000
VERSION     DACDIH010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.223"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>994)
                     /locus_tag="IV281_002907"
     CDS             complement(<1..>994)
                     /locus_tag="IV281_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8781821.1"
                     /translation="ATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVG
                     IIGAGYIGVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLS
                     ESPKKYLGRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEY
                     QQTNIKDIYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFT
                     NGFSIFNYNFYAAGLNLKTARLLSESIDYIEIEDFIRPVFLRKNSRVKLRISFEKNTK
                     RIVGVQLCSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAV"
BASE COUNT          330 a          168 c          123 g          373 t
ORIGIN      
        1 ttactgcctt taaaataaaa ttatagggtt ggctaaaact aggatgaaat aaataatcta
       61 acattttcaa tttgtctaat gtaatttgtt cttctatagc aagagaaaac attgataata
      121 gtccagataa atcttcttga ctacaaagtt gtacacctac tattctcttt gtattttttt
      181 caaagctaat tcttaatttt acacgactat tttttctcaa aaaaacaggt ctaatgaaat
      241 cttctatttc tatataatct atagactcac ttaataatcg tgctgttttt aaattcaaac
      301 ctgctgcgta aaaattgtaa ttaaatatag aaaagccatt tgtaaattgg gaaccagaag
      361 gtgtaaaatc ttgattgtca atatgttgag ttgcaatata agctgttctt agagcattag
      421 aaatagaaaa atcaacacta cttctattaa tggacgtaaa aaaagttgta gcacaatccc
      481 caatagcata tatatctttt atatttgttt gctggtattc attagttaga tatgctccac
      541 taacatctct caagagttcc tttttgccaa ttggactatt agatagaaat cctatagctt
      601 gtacgaccat atccatagag tattgggtac cacttgtagt gactattttt tcaatctttt
      661 ttcttcctaa atatttttta ggactttctg atagacaaag atgtattcca ttttttgata
      721 gtatttcttc aactttatct gaaaaactac ggtcgtaata agtcgacaac actctatctg
      781 caacatcgaa aagatatact tctttccctc tttttcttat ggcttcagca atttcaactc
      841 caatatatcc tgctccaata attcctactt ttttaatagt attatcatct aaagctttaa
      901 aaatctcttt agcatttgga aaattcttta tcgaaaaaat attagctaaa ttattccccg
      961 gtacatctaa cataatttgc tttgaacctg tagc
//
LOCUS       DACDIH010000217          975 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDIH010000217 DACDIH010000000
VERSION     DACDIH010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.224"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..695
                     /locus_tag="IV281_002908"
     CDS             <1..695
                     /locus_tag="IV281_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8781822.1"
                     /translation="YNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVST
                     AGNKKGIVVVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVT
                     VTLPNGSQYHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDS
                     EKAVSKDNKTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLG
                     LLGYKNRKKENE"
     gene            829..>975
                     /locus_tag="IV281_002909"
     CDS             829..>975
                     /locus_tag="IV281_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8781823.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFI"
BASE COUNT          388 a          128 c          198 g          261 t
ORIGIN      
        1 aatacaatcc tgaaggacaa aaagtgacta cagacttaaa taaagagcca gacgcatctg
       61 agggaattaa gaacaaagaa gatctaccaa aaggaactac ttatacttgg aaagagaaag
      121 tagatgttag tacagctgga aacaaaaaag ggattgtagt agtaacatat ccagatggat
      181 ctaaagaaga agtagaggtt actatttctg tagaagataa aaaagcaccg aataaacctc
      241 aagttgatcc tattacagag ggtgaccaaa ttgttactgg taaaactgaa ccaaatgcag
      301 aggtgacagt tacattacca aacgggagtc aataccatgg gacagctgat aaaaatggta
      361 attttacagt taaagttcct aaattagagg caggaacaaa agtgatagta actgcaactg
      421 atgaatctgg taatactagt gaacctacta atgtagttgt ttcttcaaac gaaaaagaca
      481 gtgaaaaagc cgttagtaaa gataataaga ctgacaatca aggtagcaag caaaacaaaa
      541 atagaggcaa aagcagtcct caaaagcagt cctcaaaagc ttatcctaag acgggagaga
      601 ttgatagtaa tatctttact attagtggtg gtttaatcct gttaggaact ttagggttat
      661 tagggtataa aaatcgaaaa aaagagaatg aatagaaaat caatttctta aatcttctct
      721 gaaaagagga agaatctttt tcttcctctt tttgtttttt attttgggag ataggttgtt
      781 agttaaaaat gaagatactt ttcatagaag ggaaaagaat gtgattaagt gaaaaaaata
      841 gtaattattg gatcaaacca ttcggggcta gctgcagctc gatttctaaa cttatgtgat
      901 tcaaattatg aagttattct gatagataaa aaatcagaaa ttagttactt agaatgtggg
      961 acatcgttgt tcata
//
LOCUS       DACDIH010000218          964 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDIH010000218 DACDIH010000000
VERSION     DACDIH010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.225"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            316..570
                     /locus_tag="IV281_002910"
     CDS             316..570
                     /locus_tag="IV281_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8781824.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="IV281_002911"
     CDS             571..888
                     /locus_tag="IV281_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8781825.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          332 a          164 c          200 g          268 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggt
//
LOCUS       DACDIH010000219          947 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.226, whole genome shotgun sequence.
ACCESSION   DACDIH010000219 DACDIH010000000
VERSION     DACDIH010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.226"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>947)
                     /locus_tag="IV281_002912"
     CDS             complement(<1..>947)
                     /locus_tag="IV281_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP8781826.1"
                     /translation="GFGSAFINVGILTLLSVLLAYRHKVILNGPLFASILTVTGFSFF
                     GKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFGMRFPLLIGIL
                     LGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLRLFSYSIVENT
                     LVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTTDFIANSGIGA
                     TLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSFPILVGIFIAS
                     LFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGV"
BASE COUNT          385 a          197 c          168 g          197 t
ORIGIN      
        1 acaccagcta ttacaccgta aaaactgccg taaaaaccgc ttatgggcgc gagacctgtc
       61 ccaaacacag ctgcaacaag catgccagta ctggtgatct catgaaaagt tccaaacaaa
      121 gaagcaataa atattccgac tagtatcgga aaactgtttt ttaaatgaca accaaaagca
      181 ctgaaaccca ccgcagataa aatagcaccg atgacaggtc catttaactg accgccaacc
      241 agcaaaacat aagaagacag catcagacca actaagccta tgttgatcaa agtagcacca
      301 ataccagaat tagcaatgaa atcagttgtc aattttccag atgaatcgaa aatttctcga
      361 atccccaaaa gccgaaagga attataatag aaaccaattc cgactgtcag cgaaaaaaag
      421 ccaaaaataa tccaaatgag aggaaagtga tattcattga aaaccaatgt attttcgacg
      481 attgaatagc tgaataacct caaaacagca gtaaaagtca tcgcaatcaa cccagaggtg
      541 aatccaatat tataaagtgt gaatccacga tggaaaacta aagtttgagc agctaagggc
      601 ggaagaagta gaccaattgc tatgccagct aaattcccca ataaaatacc tatcagtaaa
      661 ggaaaacgca taccaaaagt gatataacta acgaccggtg ataatgcgct accaaacagt
      721 ccgatcatga tgtactgaga aaaaggttta ttgacaaata cagcgtatag atacacacct
      781 aaaatgattg agatagaatt ataaaggttt ttgccaaaaa aagagaaacc ggtaactgtt
      841 agaatcgaag caaataaggg accgtttaaa atcaccttgt gccgataagc taaaagcaca
      901 gaaagtaagg tcaaaatccc tacatttatg aatgcgcttc cgaaacc
//
LOCUS       DACDIH010000220          947 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.227, whole genome shotgun sequence.
ACCESSION   DACDIH010000220 DACDIH010000000
VERSION     DACDIH010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.227"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>947
                     /locus_tag="IV281_002913"
     CDS             <1..>947
                     /locus_tag="IV281_002913"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP8781827.1"
                     /translation="IKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHM
                     VSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYN
                     LAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDD
                     SRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLED
                     GSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENI
                     QKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDT"
BASE COUNT          310 a          171 c          205 g          261 t
ORIGIN      
        1 atcaagaaat tgaatgatgt agatgtttta gaacacgatg gggacgtgat cggggatgcg
       61 tatgagtttc tgatcagtca atttgcttca gaagcaggga agaaagccgg agaattttac
      121 acacctcata tggtttctga tatgatggcg caaatcgtaa cgctcgatca aaaggaacga
      181 cgattcttta gtgtttttga tccaacgatg ggttcaggtt cattgatgtt aaacgtacgt
      241 aactacttaa cacacccaga taacgttaaa tatcatgggc aagaactaaa tacaacgaca
      301 tacaatctag caaaaatgaa cttgatttta catggtgtcg acgccgaaga aatgaatctc
      361 cgcaatgggg atacgttgaa taaggactgg ccaacagatg aaccttacac atttgatgca
      421 gtagtaatga acccacctta ttcggctaat tggtcagcag atacgacttt cttagatgat
      481 tctcggttta atcgttatgg gaaattagcg ccaaaatcca aagcagattt tgcgtttctt
      541 cttcatgggt tctatcattt gaaggaaaca ggtaccatgg ccatcgtctt accacacgga
      601 gttttattcc gtggcgctgc agaaggggtc atccgtcaaa aattactaga agatggcagt
      661 atttatgctg tgatcggtat gcctgcgaac ttgttctttg ggacatcgat tccaacgacg
      721 gtgattgtgt tgaagaagaa tcgtcaaact cgagatgtat tattcattga tgctagtcgt
      781 gaatttgtta aagggaaaaa tcaaaataaa ctatcggaag aaaacattca aaaaatcctc
      841 gaaacctacg ctgaaagaaa agatgtcgag aaatacgctc atttggcaac ctttgatgaa
      901 atcaaagaaa atgactataa cttaaatatc ccacgctatg tagatac
//
LOCUS       DACDIH010000221          941 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.228, whole genome shotgun sequence.
ACCESSION   DACDIH010000221 DACDIH010000000
VERSION     DACDIH010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.228"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(144..>941)
                     /locus_tag="IV281_002914"
     CDS             complement(144..>941)
                     /locus_tag="IV281_002914"
                     /inference="COORDINATES: protein motif:HMM:NF012744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8781828.1"
                     /translation="HGFHFYSGAPKINWFIYYPMERWLAHYTDVLITINHEDYTLAQK
                     LRLRKGGKAYYIPGVGIDTNRFMSSTSIRNFRESICKELNIPDTAKIILSVGELNENK
                     NHLSVIHALQKIPLHTDIYYIIAGVGEMRETLQNIANELGLHQRVFLLGYRSDIPKLL
                     AAANIYILPSLREGLNASLMEAMASGLPCIVGNIRGNVDLIEDGKGGFLINPKDEEEI
                     AGAINRLADNKLLCESMGAHNLDKIKDFDLSVVINQINNVYHEIVSK"
BASE COUNT          304 a          175 c          112 g          350 t
ORIGIN      
        1 taacgtaaat catctcaaat ttttcgttat tttgaaataa actcattatt tgacaaacaa
       61 tattttcagc gccagaaaat ctattacttt gcaacaaatg cataattttt atttttttca
      121 tattaatatc actttcaatt caattattta cttacaattt catggtaaac attatttatt
      181 tggtttatca caactgataa atcaaaatct tttatcttgt ctaaattatg tgcccccata
      241 gattcacaca ataatttgtt atcagccaat ctatttatcg caccagcaat ttcttcttcg
      301 tctttaggat tgattagaaa tccaccctta ccatcttcta taagatctac atttcctctt
      361 atattaccga caatacatgg taaaccacta gccatcgctt ccattaaaga ggcgttgagg
      421 ccttccctta atgagggtag aatatatatg tttgcagcag ctaataattt gggtatatca
      481 cttctatatc ctagtaaaaa aactcgctga tgtaatccta attcattagc aatattttgt
      541 agtgtctccc tcatttctcc taccccagcg ataatataat agatatctgt atgcagcgga
      601 attttttgta atgcatgtat tactgataaa tggtttttat tctcatttag ctctcctaca
      661 gataaaatga tttttgctgt atcaggtata ttaagttctt tacatatact ttctctaaaa
      721 ttccttattg atgtggaaga cataaaccta tttgtatcta tacccacacc tggaatataa
      781 taagcttttc cacctttcct aagacgaagt ttttgagcta aagtataatc ctcatgattt
      841 attgtaatca aaacatctgt ataatgagct agccaacgtt ccataggata atatataaac
      901 caatttattt taggagcacc tgaataaaaa tgaaatccat g
//
LOCUS       DACDIH010000222          937 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.229, whole genome shotgun sequence.
ACCESSION   DACDIH010000222 DACDIH010000000
VERSION     DACDIH010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.229"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>937)
                     /locus_tag="IV281_002915"
     CDS             complement(<1..>937)
                     /locus_tag="IV281_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP8781829.1"
                     /translation="AFINVGILTLLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNL
                     YNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFGMRFPLLIGILLGNL
                     AGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFN
                     EYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTTDFIANSGIGATLIN
                     MGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGT
                     FHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGV"
BASE COUNT          383 a          194 c          165 g          195 t
ORIGIN      
        1 acaccagcta ttacaccgta aaaactgccg taaaaaccgc ttatgggcgc aagacctgtc
       61 ccaaacacag ctgcaacaag catgccagta ctggtgatct catgaaaagt tccaaacaaa
      121 gaagcaataa atattccgac tagtatcgga aaactgtttt ttaaatgaca accaaaagca
      181 ctgaaaccca ccgcagataa aatagcaccg atgacaggtc catttaactg accgccaacc
      241 agcaaaacat aagaagacag catcagacca actaagccca tgttgatcaa agtagcacca
      301 ataccagaat tagcaatgaa atcagttgtc aattttccag atgaatcgaa aatttctcga
      361 atccccgaaa gccgaaagga attataatag aaaccaattc cgactgtcag cgaaaaaaag
      421 ccaaaaataa tccaaatgag aggaaagtga tattcattga aaaccaatgt attttcgacg
      481 attgaatagc tgaataacct caaaacagca gtaaaagtca tcgcaatcaa cccagaggtg
      541 aatccaatat tataaagtgt gaatccacga tggaaaacta aagtttgagc agctaagggc
      601 ggaagaagta taccaattgc tatgccagct aaattcccca ataaaatacc tatcagtaaa
      661 ggaaaacgca taccaaaagt gatataacta acgaccggtg ataatacgct accaaacagt
      721 ccgatcatga tgtactgaga aaaaggttta ttgacaaata cagcgtatag atacacacct
      781 aaaatgattg agatagaatt ataaaggttt ttgccaaaaa aagagaaacc ggtaactgtt
      841 agaatcgaag caaataaggg accgtttaaa atcaccttgt gccgataagc taaaagcaca
      901 gaaagtaagg tcaaaatccc tacatttatg aatgcgc
//
LOCUS       DACDIH010000223          932 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.230, whole genome shotgun sequence.
ACCESSION   DACDIH010000223 DACDIH010000000
VERSION     DACDIH010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.230"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            105..>932
                     /locus_tag="IV281_002916"
     CDS             105..>932
                     /locus_tag="IV281_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8781830.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
BASE COUNT          291 a          125 c          191 g          325 t
ORIGIN      
        1 gcctattcag gtctttcact aattggcgtg acaatttttg cagctctgat tgcctttgta
       61 atggatcaag ttaaatatgg aaaaagagaa gaggcgcatg gttaatggaa aatagtacag
      121 aaaaaaaacg tttaatcaca aaaaaagatc ttaataagat gtgtcttcgt tcatggacaa
      181 tcatgtgttc gtggaattat gaaagacaaa tgcatatggg atttatgtat gggatggcgc
      241 caattttaaa taaagtttat aaaaatgatg aagagaaaaa aagagaagct tatactcgtc
      301 atatggcatt ttttaactgt acgcctcaaa tgacttcatt tattatggga ctttgtgctt
      361 ctatggaaga acaaaatgct caagcagaag aaggaaactt taatactgaa tctatctcca
      421 tgattaaaac cagtttaatg ggtccttttg ctggaattgg tgattcattt ttccaaggta
      481 ctttaagaat tattacattt gggattggat tgtcttttgc taagcaaggt agtattttag
      541 gaccgatttt agctgttggc ctttttgcta taccttctat tttagctgtt ttttacggcg
      601 gtaaattagg atatgagtct ggcaataaat accttactaa attgtatcaa gaaggtctta
      661 tggatcgagt aatgcaattt gcttctatca ttggtttggc tgtagtaggt ggaatggtgg
      721 ctagcatggt gccaattgtc acccctttaa aattcactac agggggaaca gaacttgttt
      781 tacaagactt acttgattcc attattcctg gattattacc acttgtatgt actttgggaa
      841 tatataaatt gattaagaaa aatgttagta caaatatgct cttattaggt tgtattttgg
      901 cagggtttgt tttaagtata ataggtatct ta
//
LOCUS       DACDIH010000224          925 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.231, whole genome shotgun sequence.
ACCESSION   DACDIH010000224 DACDIH010000000
VERSION     DACDIH010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.231"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..518)
                     /locus_tag="IV281_002917"
     CDS             complement(<1..518)
                     /locus_tag="IV281_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP8781831.1"
                     /translation="MTINEVIYSPKHPLFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLGNLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPL"
     gene            complement(569..>925)
                     /locus_tag="IV281_002918"
     CDS             complement(569..>925)
                     /locus_tag="IV281_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8781832.1"
                     /translation="LMRYYGGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGA
                     MTISMVKFNTVLSFSMKGEELMNVQEILDSIFKGIVPLLVTLGCFKLLDKKVGVIKII
                     FGMVVLGILLSLLGIA"
BASE COUNT          293 a          196 c          147 g          289 t
ORIGIN      
        1 tacaaaggca catctttcac tgaaacttga aggctttcgt gtgtttcatt cacaatgtaa
       61 acctgttgaa caagcatact taaacgctta ttagccagta ttggagcaaa ggatactaca
      121 atgaccttaa acgtcgaata atgatgaata tatctaggca aacagtcttg aatcacatcg
      181 acttcaatac gatgatccga ttctgacaaa tggtagacaa tcccgtttac ttcaaccttt
      241 aaggataacg aattggtttg agaaagctgt ttattgaccc aaacacctgg tttttcctca
      301 gttagtaaat tttctcgata aaaggtcaac gtttgaatcg tcccgcaaac atcaaggttt
      361 cctaatgtct tttgattact tacatcgaag gtcaatccct ttacatcagg aatattccga
      421 tagtagctgt attcgtttga agtagtcaca tttccgtcgt ttaactcagc attaaataat
      481 gggtgtttgg gtgaataaat tacttcatta atagtcatct tttccctcct tgctaaaaaa
      541 actgcagcct agtagctgca gcttcctact atgcgattcc aagtaatgac aataaaattc
      601 ccaagacgac cattccaaaa ataattttga tgacgccgac ttttttatct agtaatttga
      661 aacagcccaa tgttaccaat aacggtacaa ttcctttaaa aatactatcg aggatttctt
      721 ggacattcat tagttcttcg cccttcattg aaaagctcaa aacggtattg aatttgacca
      781 tactgatcgt cattgcgcct accatgatca gacccaagat actagcagct tttgtcagaa
      841 tttcaattaa cccgttcgca tacagctttt caatatattt agagcctaat gaatagccta
      901 aaacgccgcc atagtatcgc atcaa
//
LOCUS       DACDIH010000225          925 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.232, whole genome shotgun sequence.
ACCESSION   DACDIH010000225 DACDIH010000000
VERSION     DACDIH010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.232"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..518)
                     /locus_tag="IV281_002919"
     CDS             complement(<1..518)
                     /locus_tag="IV281_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP8781833.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPS"
     gene            complement(569..>925)
                     /locus_tag="IV281_002920"
     CDS             complement(569..>925)
                     /locus_tag="IV281_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8781834.1"
                     /translation="LMRYYGGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGA
                     MTISMVKFNTVLSFSMKGEELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKII
                     FGMVVLGILLSLLGIA"
BASE COUNT          293 a          194 c          150 g          288 t
ORIGIN      
        1 tacgaaggca catctttcac tgaaacttga aggctttcgt gtgtttcatt cacaatgtaa
       61 acctgttgaa caagcatact taaacgctta ttagccagta tgggagcaaa ggatactaca
      121 atgaccttaa acgtcgaata atgatgaata tatctaggca aacagtcttg aatcacatcg
      181 acttcaatac gatgatccga ttctgacaaa tggtagacaa tcccgtttac ttcaaccttt
      241 aaggataacg aattggtttg agagagctgt ttattgaccc aaacacctgg tttttcctca
      301 gttagtaaat tttctcgata aaaggtcaac gtttgaatcg tcccgcaaac atcaaggttt
      361 gctaatgtct tttgattact tacatcgaag gtcaatccct ttacatcagg aatattccga
      421 tagtagctgt attcgtttga agtagtcaca tttccgtcgt ttaactcagc attaaaaaat
      481 gggtgtttgg gtgaataaat tacttcatta atagtcatct tttccctcct tgctaaaaaa
      541 actgcagcct agtagctgca gcttcctact atgcgattcc aagtaatgac aataaaattc
      601 ccaagacgac cattccaaaa ataattttga tgacgccgac ttttttatct agtaatttga
      661 aacagctcaa tgttaccaat aacggtacaa ttcctttaaa aatactatcg aggatttctt
      721 ggacattcat tagttcttcg cccttcattg aaaagctcaa aacggtattg aatttgacca
      781 tactgatcgt cattgcacct accatgatca gacccaagat actagcagct tttgtcagaa
      841 tttcaattaa cccgttcgca tacagctttt caatatattt agagcctaat gaatagccta
      901 aaacgccgcc atagtatcgc atcaa
//
LOCUS       DACDIH010000226          910 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.233, whole genome shotgun sequence.
ACCESSION   DACDIH010000226 DACDIH010000000
VERSION     DACDIH010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.233"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..355
                     /locus_tag="IV281_002921"
     CDS             <1..355
                     /locus_tag="IV281_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781835.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="IV281_002922"
                     /pseudo
     CDS             615..910
                     /locus_tag="IV281_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          353 a          106 c          190 g          261 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa
//
LOCUS       DACDIH010000227          900 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.234, whole genome shotgun sequence.
ACCESSION   DACDIH010000227 DACDIH010000000
VERSION     DACDIH010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.234"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            54..425
                     /locus_tag="IV281_002923"
     CDS             54..425
                     /locus_tag="IV281_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781836.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(681..>900)
                     /locus_tag="IV281_002924"
     CDS             complement(681..>900)
                     /locus_tag="IV281_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8781837.1"
                     /translation="VGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQALLGSWGP
                     SIAGMAIVPSLIGAIILVLIVSFFARKL"
BASE COUNT          266 a          176 c          142 g          316 t
ORIGIN      
        1 caaatatcgt tattttaaaa tgagggcgaa ctcacacaat taggaggaat agtatgccat
       61 ttattgttta cttttttctt tcattactcg tgatctatat tccattacca acatttttcc
      121 tgctcgtcaa tcgtctagca gagcaacatg atacgattgc tgctcttacg aaactttgtt
      181 tacttctatt tgtttgtcta gattacttta cgagctatta ttggctaaaa cagacaagag
      241 taaaaaagag atattatttc ttccttgtct ttttcaatct ctttgagttt ctgatccatt
      301 gctttttact tcatcagtat catacgatcc atttgtggat catctgggtt ttcaaagtgg
      361 tcgtattatt ttgtatgttc acctttccta tctcaagaaa atttagggag gcattgtttt
      421 attaaagaag cttctctgta tcaataaaga cacatttaag gaaaaacttt taaaaatcta
      481 attgggaatt tgcccgcata aataagaggc tgtgacaaaa gtcttgtcac agcctcttat
      541 tccgtataaa cagtgttcaa aagcttgcat cttcgataat atgcccaacc aaatgaaaaa
      601 tatgagaagc tatttttgtt tggctgttct tattacttga tgaagaacag ctttttcacg
      661 acctcttttt aaaagtcttt ttacagtttt cgagcaaaga atgatacgat tagtactaaa
      721 atgatggcac caatcaatga tggcacgatt gccattcctg caatacttgg tccccaagaa
      781 cctaataatg cttgaccaag tgctgatcca actaaacctg ctacgacatt tgcaataatc
      841 cccatcgaag aacctttatt cgtgattgct cctgcaattg ctccgataat tcctccgacg
//
LOCUS       DACDIH010000228          890 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.235, whole genome shotgun sequence.
ACCESSION   DACDIH010000228 DACDIH010000000
VERSION     DACDIH010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.235"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..888
                     /locus_tag="IV281_002925"
     CDS             <1..888
                     /locus_tag="IV281_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP8781838.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DACDIH010000229          885 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.236, whole genome shotgun sequence.
ACCESSION   DACDIH010000229 DACDIH010000000
VERSION     DACDIH010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.236"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            48..356
                     /locus_tag="IV281_002926"
     CDS             48..356
                     /locus_tag="IV281_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP8781839.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            383..682
                     /locus_tag="IV281_002927"
     CDS             383..682
                     /locus_tag="IV281_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAP8781840.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
BASE COUNT          327 a          118 c          197 g          243 t
ORIGIN      
        1 ctttaataga agattgggct atttttatgg caaaggaggg aaaagaaatg tttcaaaata
       61 aaggacaacg ttatgtcaca aagggagtaa tggacagttt gccggtggaa ttacaagctt
      121 tatgttggaa tctaatcgat caaaacgttc aaaagcaatt tcatttagac tatcttcaaa
      181 tctttgaatt ttctacggaa aaaggaaatc aaaagttggt tcatcgtcaa gaagaaccag
      241 aagcaagaaa agagtatctg atttcaccta agcttcgttt gaaatcagtg tctcaaaaaa
      301 tatgggtcat tgattcgggt gagtatcaaa cgatgttatt accagaagaa tactgatatc
      361 taaaagaaag gaacgaaagg acttgttaga aacggctatc gaagtactgg aaaagtgtgc
      421 acagctagtc actactcctg aagagtgggg ctatgaatcc gttacgatgg aaaaagagga
      481 aattgaaatg ggtatgcttc ctgaagatgt tactctacca cgtttagtga tgacacatct
      541 ttatatttat tgcgcaccag aagatggaaa agactatgta gtttatttca ttacagatat
      601 taccagccag cgagaatttg ttcgtgggtt actggtagaa ggacgattgg tttggagtca
      661 gattggagga acgaatgaat aagatcgatt atttgagaat gattgcgaaa tgtttagaag
      721 atatccttac agcgattcat caaattctga agaaaaaagg agaaagaata tgaaatattt
      781 tcaactagta ttgacactta taacggcaat ggtgattgaa ttgcttgaac aaaaattagc
      841 ataggagaaa aagagatgat tgaaaaagaa ttagtgacca cacaa
//
LOCUS       DACDIH010000230          884 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.237, whole genome shotgun sequence.
ACCESSION   DACDIH010000230 DACDIH010000000
VERSION     DACDIH010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.237"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>884
                     /locus_tag="IV281_002928"
     CDS             <1..>884
                     /locus_tag="IV281_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP8781841.1"
                     /translation="CQVSLTTVIRTLKEFKSYLPKQSKKILPRVLMVDEFRSHASIED
                     KMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQLTKRVL
                     PNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKSQAEKLKKNWRFLLKNRANIN
                     HYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAYPFFHELVEAFRDKDPDLFFS
                     LLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNGKIEAKNTHIKTMKRVSYGFK
                     SFENMRIRIFLINQLINV"
BASE COUNT          298 a          178 c          166 g          242 t
ORIGIN      
        1 ctgtcaggta tctttgacca ccgttattcg tacattgaaa gagtttaaaa gctatttacc
       61 aaagcaatct aagaagatcc tccccagagt attgatggtt gacgaatttc gttcgcatgc
      121 ttccatagaa gataagatga gctttatttg cgcggatggc gaaacaggaa aattaataga
      181 tgttttgcct acgcgtaaat tacctcgatt aacaagctat ttcttaggtt gtaccaatcc
      241 agaagaagta gaattcttgg tgacagacat gaacgccgcc tacttccagc tcaccaaacg
      301 tgttctgcca aatgcgaaag tcgtgattga tcggtttcat attgtcaaac acatgaatca
      361 agcgttcaat gagttgcgta tccgtgaaat gaatgaactt cgtaaagccg gacagaaaag
      421 ccaggcagaa aaactgaaaa agaactggcg ctttttgcta aaaaatcgtg caaacatcaa
      481 ccattatgaa tacaaaacat ggaaaagttt ccgagcacca aaatacccat ttcttactga
      541 agcaatgatg attgatcgat tgcttgagtt ttctccgccc ctaaaggagg cgtatccctt
      601 ttttcatgaa ttagttgaag cctttcgaga caaagaccct gacttatttt tctccttatt
      661 ggcagaactt cccgaaacgt tagatgacag ctttcgggaa aagcttcaaa accttctgac
      721 ctatgaagaa ggcatcacca acgcaatgat ctatccttat tccaatggaa aaatagaagc
      781 gaagaatacc cacataaaga caatgaaacg agtatcctac ggatttaaat catttgagaa
      841 catgagaatt agaatctttt tgatcaatca attaatcaat gtaa
//
LOCUS       DACDIH010000231          863 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.238, whole genome shotgun sequence.
ACCESSION   DACDIH010000231 DACDIH010000000
VERSION     DACDIH010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.238"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..373
                     /locus_tag="IV281_002929"
     CDS             <1..373
                     /locus_tag="IV281_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8781842.1"
                     /translation="LIELTHLLEQQSFSSADGCAENEMLRVLDYIDKHFTSCTLKETA
                     AHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQETSFSLEEIANRIGYKQTT
                     SLFKLFKKYSTCTPNEWRNKH"
     gene            669..>863
                     /gene="ldh"
                     /locus_tag="IV281_002930"
     CDS             669..>863
                     /gene="ldh"
                     /locus_tag="IV281_002930"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Converts (S)-lactate and NAD(+) to pyruvate and
                     NADH; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8781843.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWS"
BASE COUNT          302 a          115 c          164 g          282 t
ORIGIN      
        1 actgatcgaa ttgactcatt tattagaaca acaaagcttt tcatcagcag atggatgcgc
       61 agaaaatgag atgcttagag tattagatta tatcgataag cattttactt cttgtacgtt
      121 aaaagaaaca gcagctcatt ttggctacaa tccggtctat cttagtaata aattgaaaat
      181 gtattttgat gcttcctttc aagaactggt tctaaagaaa aaattcgact ttgcgttgga
      241 actgattcaa gaaacgagtt tttcgttgga agaaatcgct aatcgtatcg gatataaaca
      301 aacgacttct ttattcaaat tattcaagaa gtattcaaca tgtactccca atgaatggag
      361 aaataaacat taagaagaaa atctttcact aaagcatatg aaggaaaatt gttttaggtg
      421 cgattgctgt cttattatat cgttgcagcg aatttttttg gatttatttt cagttctaaa
      481 cttttggttg tattatcagg ggatataggt gtaaacattt gtcatttatc tgttatgcgt
      541 attcaggaat tcgtttccat caaaaattat aataacaact attataatta acatgttgat
      601 aaaatgaata aacaagacta tacttttcat gaaagtaaca aatagttatg aaaaggagct
      661 gtttattcat gaaaaaaaca agtcgtaaag tagtaatcgt cggtacaggt tttgttggaa
      721 caagtatcgc ttatgcaatg atcaaccaag gaatctctaa tgaattagta ttgatcgatg
      781 tgaatcaaga aaaagcagaa ggggaagcat tggacctttt agatggtatg gcttggggcg
      841 atgaaaatgt ggctgtatgg tca
//
LOCUS       DACDIH010000232          848 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.240, whole genome shotgun sequence.
ACCESSION   DACDIH010000232 DACDIH010000000
VERSION     DACDIH010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.240"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(192..590)
                     /locus_tag="IV281_002931"
     CDS             complement(192..590)
                     /locus_tag="IV281_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP8781844.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(587..823)
                     /locus_tag="IV281_002932"
     CDS             complement(587..823)
                     /locus_tag="IV281_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8781845.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
BASE COUNT          262 a          154 c          120 g          312 t
ORIGIN      
        1 agcccactag ttctaaacca ctatttccta ctattcactc ttttgtattc cttttaaatc
       61 aattatttca aaattaatcc atattttcca tatttttatg aaaaaactat tttgtatgat
      121 taaacataaa aaaatcagca tattccttaa tgacatgctg atctaattga gaaataaagt
      181 ttttaattcg ttcattttat tgcggtttgt cgtaaataac tggttatctt ggactttagt
      241 gaatcgaaat ccaatgaact agtggtaatc attatgataa aatcaacagc ttcttgacga
      301 gaaaaagcaa ctggacaatt attcaattga agaaacaaca acatggcaac aagagcagtt
      361 cgtttattgc cattatgaaa aggatgtttt ttagctaaat taattgctaa aatcgccgct
      421 ttttcataga tttcaggata gagttcttga ttgaatactg cttgtgaagg ctgtttgacg
      481 gccatatcta atgcatttgc atcttttaca ccgatcattt cgcctggcga gtgacgtaaa
      541 ataactttcg cgttgatttt gataatttct ttagtcgaaa gatacctcat aaatcaacca
      601 aatcttttaa cgcgtcttca tattgattca tgatcgaatc aactaatgaa tcaatatcag
      661 gttcttcttg tgctttgatc attcgtacag aaccatcaga ttgaaaaaca taactgattg
      721 cgtcacctgt ctgcacaccc atgaattctt ttacttctgg tgggatagtt gttacatcgc
      781 tattgccaga ttttctaatt ttatactttt tcattttttt catatttcgc caccctctat
      841 accattct
//
LOCUS       DACDIH010000233          836 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.241, whole genome shotgun sequence.
ACCESSION   DACDIH010000233 DACDIH010000000
VERSION     DACDIH010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.241"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>836)
                     /locus_tag="IV281_002933"
     rRNA            complement(<1..>836)
                     /locus_tag="IV281_002933"
                     /product="23S ribosomal RNA"
BASE COUNT          183 a          261 c          188 g          204 t
ORIGIN      
        1 tctctcaggg atcttacttt cttaaagaaa tgggaaatct catcttgagg tgggcttcac
       61 acttagatgc tttcagcgtt tatcccttcc ctacatagct acccagcaat gcccttggca
      121 gaacaactgg tacaccagcg gtaagtccat cccggtcctc tcgtactaag gacagctcct
      181 ctcaaatttc caacgcccgc gacggatagg gaccgaactg tctcacgacg ttctgaaccc
      241 agctcgcgtg ccgctttaat gggcgaacag cccaaccctt gggaccgact acagccccag
      301 gatgcgacga gccgacatcg aggtgccaaa cctccccgtc gatgtggact cttgggggag
      361 ataagcctgt tatccccagg gtagctttta tccgttgagc gatggccctt ccatgcggaa
      421 ccaccggatc actaagcccg actttcgtcc ctgctcgact tgttggtctc gcagtcaagc
      481 tcccttctgc ctttacactc ttcgaatgat ttccaaccat tctgagggaa cctttgggcg
      541 cctccgttac cttttaggag gcgaccgccc cagtcaaact gcccatctga cactgtctcc
      601 caccacgatt agtggtgcgg gttagagtgg ccataacgca ggggtagtat cccaccagcg
      661 cctccatcga aactagcgtt ccgatttcta cggctcctac ctatcctgta catgcggtac
      721 agacactcaa tatcaaacta cagtaaagct ccatggggtc tttccgtcct gtcgcgggta
      781 acctgcatct tcacaggtac taaaatttca ccgagtctct cgttgagaca gtgccc
//
LOCUS       DACDIH010000234          833 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.242, whole genome shotgun sequence.
ACCESSION   DACDIH010000234 DACDIH010000000
VERSION     DACDIH010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.242"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..537
                     /locus_tag="IV281_002934"
     CDS             <1..537
                     /locus_tag="IV281_002934"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781846.1"
                     /translation="VMAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNG
                     QPMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEEIKEMGYNKYGGLD
                     MTIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQA
                     IIDSGRVTQWKKIRPFIF"
     gene            564..>833
                     /locus_tag="IV281_002935"
     CDS             564..>833
                     /locus_tag="IV281_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781847.1"
                     /translation="MNENGEFEESKKYQATSEEEKAAVRELLEEAVPTDFLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYYK"
BASE COUNT          354 a          127 c          187 g          165 t
ORIGIN      
        1 gtcatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccagaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gagcgaatcg aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacaa
      241 gtagaaaaac ttgaaagaca agaagagata aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgagaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc ggaaaaagga aaagaaaacc ttcttcctga agtacaagca
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaatcc gaccatttat tttctgaaag
      541 gctatcgaaa agtcgcttta gaactgaatg aaaacgggga atttgaggaa tctaaaaaat
      601 accaagccac gtcggaagag gaaaaagcag ctgttcgtga attacttgaa gaggctgtac
      661 caactgattt tttgaaggag aaagaaaacg atcattttta tcgagtagaa ttcaacgaaa
      721 atgatccaga aaaaggattg aaaagttatg gtggggagat cgtaacattc gaattgatcg
      781 atcaactcaa aacgttggat aaccaatacg ccgcagaagc aggttattat aaa
//
LOCUS       DACDIH010000235          830 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.243, whole genome shotgun sequence.
ACCESSION   DACDIH010000235 DACDIH010000000
VERSION     DACDIH010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.243"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..289
                     /locus_tag="IV281_002936"
     CDS             <1..289
                     /locus_tag="IV281_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781848.1"
                     /translation="GDLSDYYNCKREVIVDNRYDQSKDDKRIIKIGDIVFPEKGIAYE
                     VVSINSFRYEFVDLVVEYSVRLIVPWSKEEINEALKDERKSTFKVLEGEKK"
     gene            286..765
                     /locus_tag="IV281_002937"
     CDS             286..765
                     /locus_tag="IV281_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781849.1"
                     /translation="MKTDLTRQAEKCLWHETNRMGVFGCFEVTIGWDGRERVDYITYS
                     VDNTVRCYEIKVTLSDLKSKAKQTFIGDYNYLVVTSDLWEKMLQDDTLMRKYYKQGVY
                     VFNEKYPSMISVKKARKQKVSLGTRSTVLESMVRSMNREVKKLYKVKPFWEIVEIPE"
BASE COUNT          316 a           92 c          183 g          239 t
ORIGIN      
        1 cggagacttg agtgattatt ataactgcaa aagagaagtt attgtggata atcgatacga
       61 ccaatcaaaa gatgataagc gaataataaa aataggagat attgtctttc ctgagaaagg
      121 gatagcatat gaagtagtgt ctatcaatag ttttcgctat gaatttgttg atttggttgt
      181 cgaatattct gttcgattga tcgttccgtg gagtaaagaa gaaattaatg aagcattaaa
      241 agatgaacga aaaagtacat tcaaagtttt agaaggtgaa aaaaagtgaa aacagacctt
      301 actagacaag ctgagaagtg tttatggcat gaaacaaatc gcatgggtgt atttggttgt
      361 tttgaagtaa ctatcggttg ggatggcaga gaaagagtag attatataac ttattcagta
      421 gataacacag ttcgatgtta tgagatcaaa gttactttat ccgatttgaa aagcaaggcg
      481 aaacagacat ttatcggcga ttataactat ttagtcgtta ctagtgattt gtgggagaaa
      541 atgctacaag acgacacact catgcgtaaa tattataaac aaggcgtata cgtgtttaat
      601 gaaaaatatc ctagcatgat aagtgttaag aaagcaagga aacaaaaagt ttcgctaggt
      661 acaagatcaa ctgtattaga aagcatggtt agatcgatga atcgtgaagt taagaaatta
      721 tataaagtaa aaccattttg ggagatagta gagataccgg aatgatgagt aaggaatatt
      781 atatctattc atatatagta ttaactgatt atgaggagga tacagcaaat
//
LOCUS       DACDIH010000236          820 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.244, whole genome shotgun sequence.
ACCESSION   DACDIH010000236 DACDIH010000000
VERSION     DACDIH010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.244"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..455)
                     /locus_tag="IV281_002938"
     CDS             complement(<1..455)
                     /locus_tag="IV281_002938"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781850.1"
                     /translation="MAFQVYLQRVYILSTGVIIGHYAEMGLNRIAYAGLMGDFSFAGL
                     YLATGCLYAFLKYVDLKNWKLLFFIIIEVFLLGAILCVSARTGLVSLFVTMTLYYLFN
                     INRISVPHLLVLIGGIITTPYILVMLMAGRSGQSLLDSSGRLENYMSSL"
BASE COUNT          338 a          135 c           94 g          253 t
ORIGIN      
        1 tttaaagagg acatatagtt ttctaatctg cctgaagaat ccaaaagact ttgtccagaa
       61 cgacctgcca ttaacattac tagtatataa ggtgtagtta tgattccacc tattagaact
      121 aaaagatgag gaacactaat tctatttata ttaaatagat aatacaatgt cattgtgaca
      181 aacaaggaaa ctaaccctgt tcttgcacta acacataaaa ttgcacctaa taaaaatact
      241 tctattatta taaagaataa taatttccaa ttttttaaat ccacatattt tagaaaagca
      301 tatagacagc ctgtagcaag atataaccct gcaaaactaa aatctcccat caaacctgca
      361 tatgcaattc tatttaaacc catttctgca taatggccta ttattacacc agtagataaa
      421 atatatactc gttgcaaata tacctgaaac gccatagata ctactcctat gaaatagaaa
      481 ttccacccaa tcatagttaa gtttttgcta tacattttaa aacgttttcc tattaaaaaa
      541 ctacagataa acaacgaaat agtcattaat tgctttaatg catctattaa aaaaggtaag
      601 aacatagacg tcgatattcc aaaacaaata aataaaaata aaataatcaa gtatgaacga
      661 tttaatgttc cttctataat aatatataac atcaaaatta ttattattag attatgccac
      721 gtcaatggta agctagaata actagttcct aatatagata tagttgatgt tggaatgaaa
      781 agaattgatg ttaacaataa gctatctgct aaacaaaaat
//
LOCUS       DACDIH010000237          817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.245, whole genome shotgun sequence.
ACCESSION   DACDIH010000237 DACDIH010000000
VERSION     DACDIH010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.245"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>817
                     /locus_tag="IV281_002939"
     CDS             <1..>817
                     /locus_tag="IV281_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HAP8781851.1"
                     /translation="KKNVTGANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFL
                     FTKQSGSIKDYKGTPRTVLVQRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAEN
                     LGPLGVRKMTLIDNDCFQAENLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKFPYL
                     CVEVESMNVLDLISMQPGFFKEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPY
                     GIGGHTLFINYSLNQKGCFNCLYTDSNSKNRCSNRASLVKEGQIFERTLASCRS"
BASE COUNT          285 a          104 c          161 g          267 t
ORIGIN      
        1 taaaaaaaaa tgtaacaggt gcaaataaaa gatttttttt aaaacttgaa aaaagaaaga
       61 atagacataa tgttttactt attctagata tcccaattga agaaaagtca aataatgtca
      121 taattggatt tctttttaca aaacaatctg gatctataaa agattataag ggtacgccta
      181 ggactgtatt agttcagcgt catgatgaat tatatttggt gaatagaacg agcggagaaa
      241 actatggatt gggtgctaat atagctattg ttggggtagg gtcagttggc agtaaaattg
      301 ctgaaaatct tggtccatta ggtgtgagaa agatgacact catagacaat gattgttttc
      361 aagctgaaaa tttgtatcga aattcacttg gtgcagatag tttaaaaaat gcccgatctc
      421 agaataattt ttttaaagtt gatttaatgc aggaggagct cgaaaaaaaa ttcccatacc
      481 tttgtgttga agtggagtca atgaacgttt tagacttaat tagtatgcaa cctggttttt
      541 tcaaggaata tgactatgtt tttgtttgca taggagatac aatggcaagc ctggctttaa
      601 ataattattt ttccaaaagt agtcaaaaag ttttctattc ttggctggag ccatatggca
      661 ttgggggaca cactctattt ataaattatt ctctaaatca aaaaggttgt ttcaattgtc
      721 tttatacaga ttcaaactct aaaaataggt gttcaaatag agcttctctg gtaaaagaag
      781 gacaaatttt tgaaaggact ttagcgagtt gccgctc
//
LOCUS       DACDIH010000238          810 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.246, whole genome shotgun sequence.
ACCESSION   DACDIH010000238 DACDIH010000000
VERSION     DACDIH010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.246"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>810
                     /locus_tag="IV281_002940"
     CDS             <1..>810
                     /locus_tag="IV281_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP8781852.1"
                     /translation="GLNEAVNIKDFNLMKWIGANSFRTSHYPYSEEIMRLADREGIVV
                     IDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKTVIKELIERDKNHPSVVMWS
                     IANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYLKSTPDVCKVGDIVDVLCLN
                     RYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTEYGADTVAGFHDTVPTMFTE
                     EYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILRVQGNKKGIFTRDR"
BASE COUNT          291 a          101 c          181 g          237 t
ORIGIN      
        1 ggtttgaatg aggctgtaaa tattaaagat tttaatttaa tgaaatggat aggtgctaat
       61 tcttttagaa catcacatta tccttactca gaagaaatta tgcgattagc agatcgagaa
      121 ggtattgtag ttattgatga agtacctgca gtagggttac atttaaattt catggccact
      181 ttattagatg ataatgtgga aaaacataat acgtggaaag agatacgaac agaaaattct
      241 cataaaacgg tgatcaagga attgatagaa cgcgacaaga accacccaag tgttgtaatg
      301 tggtctattg caaatgaacc agattcggat tccgaaggtg ctaaagaata ttttgaacct
      361 ttagttgaat taactaaaga acttgatccg cagaaaagac ctgtaacaat cgttacttat
      421 ctaaaatcaa cgccagatgt ttgtaaggta ggagatattg tcgatgtttt atgtttaaat
      481 cgttattatg gttggtatgt ttcgggaggg gatctggcac atgcaaaata tctgttgaaa
      541 aaagagctag atggatggat ggaaagatgt cctgatacgc caataatgtt tacagaatat
      601 ggagcagata cagtagctgg atttcatgat acagtaccaa caatgtttac agaagagtat
      661 caagtggctt attacgaagc aaatcacgaa gtaatagata catgtgaaaa ttttgttggt
      721 gaacaagtgt ggaattttgc agattttgca acaagtcaag ggattttaag agtgcaagga
      781 aataagaaag gaatctttac tagagataga
//
LOCUS       DACDIH010000239          803 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.247, whole genome shotgun sequence.
ACCESSION   DACDIH010000239 DACDIH010000000
VERSION     DACDIH010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.247"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            216..>803
                     /locus_tag="IV281_002941"
     CDS             216..>803
                     /locus_tag="IV281_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8781853.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQ"
BASE COUNT          283 a          115 c          126 g          279 t
ORIGIN      
        1 taaaaacatt ctaaattaaa tcagtaaaaa tttttattct aagattttta ttagaattac
       61 gcctaatttg tgtctctcag ggcaattcta agaacgtttc aagagatagc gacactatcg
      121 ttatcgaaaa aggcgaaaac ctatctcatt ttctgctaat gtaagttggt ttagcttttg
      181 ttaatatgac tttatgaaaa tggaggtaat taaatatgat ttttcctaaa gttaatgaag
      241 caagcttgaa taaaggatcc ttttttcata tctatgaaaa cgaagatttt gaactgtata
      301 atatcctttc acaaaaagag gtcatcgaaa accaaataat ctgccatcca attatttaca
      361 aatctatttt caagtatgca ttttttgctt ccaatgattt tcaaagaatg gagattaccg
      421 actgtatatt cgaaaactgt gatttttcag gctgtatatt gacaaaaagc atgttgcatc
      481 gcgtgaaatt tgtcaattgt aaattaacag gtactaaatt gatggaaagt tatattggga
      541 acacgttatt cgagaattgt aaaatggact atgtaaattt atctggttca aacataaaag
      601 aaagtacctt tgagcactct gtcttatcaa gcgcagattt tgtagactgt tccctcacta
      661 aaataatgtt ttctactaat gatttagaaa atgctagctt ttttgatact gatttaaaaa
      721 atatcgattt aagtagcaac tcctttgaaa tgattgaagt acgctcggaa catctaaaaa
      781 attgtacgct taatcagttg caa
//
LOCUS       DACDIH010000240          791 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.248, whole genome shotgun sequence.
ACCESSION   DACDIH010000240 DACDIH010000000
VERSION     DACDIH010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.248"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>791
                     /locus_tag="IV281_002942"
     CDS             <1..>791
                     /locus_tag="IV281_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781854.1"
                     /translation="KNTQSIYTTGDLSFHLSRIKGLSTIFTSPINYETFNYTGYGVNY
                     FYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNLCTTLVAYHYGERFLKQKKAAFLFSC
                     LYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFYAYQIISGKEEKYPSVKLALSMSLLV
                     YSHVLSTLMSTALIIIFIFIRLVSKGFKNADFIAIFKKLFSAAGMTLVLTSAFWYPMF
                     EQMLYQKINKPSVTNLYAHASNVFDSLTEAMNNDLTTYSMGLVGL"
BASE COUNT          237 a          130 c          133 g          291 t
ORIGIN      
        1 tcaaaaatac acagagtatt tataccactg gagatctttc ttttcactta tctcgaatca
       61 aggggttatc gactattttt accagtccga ttaattatga gacattcaat tatactggat
      121 atggtgtaaa ttatttttat ccctttctga ctttttttcc agctgtaatg ttatattgga
      181 tgacaaaaaa tttgattgta agctatatta tttacgtttg gttattgaac ttgtgtacaa
      241 cactcgtagc ttatcattat ggcgagcggt ttttaaaaca aaaaaaagcg gcatttttat
      301 ttagttgtct ctatattttc tctgcttatc ggacagtaga catctactat cgttccgcaa
      361 ttgccgaagc tatcgcaata acacttgtta tccctgtatt attttatgct tatcaaatca
      421 tctctggaaa agaagaaaag tatccgtctg taaagctagc attgagtatg agtttgctag
      481 tgtacagcca tgtactatca acattaatga gtacagcatt aattatcatt tttattttta
      541 ttcgtttggt ttctaaaggt ttcaagaatg cagattttat tgctatattt aaaaaattat
      601 ttagtgcggc aggaatgaca ttggttctga cgagtgcttt ttggtatccg atgtttgaac
      661 aaatgctcta ccaaaaaatc aacaaaccta gcgtgacaaa tttatatgct catgcatcta
      721 atgtgttcga tagtctgact gaagctatga acaacgactt aactacttac tcaatgggac
      781 tagttggtct g
//
LOCUS       DACDIH010000241          790 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.249, whole genome shotgun sequence.
ACCESSION   DACDIH010000241 DACDIH010000000
VERSION     DACDIH010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.249"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..355)
                     /locus_tag="IV281_002943"
     CDS             complement(<1..355)
                     /locus_tag="IV281_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8781855.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGMDRLQLT"
     gene            complement(479..>790)
                     /locus_tag="IV281_002944"
     CDS             complement(479..>790)
                     /locus_tag="IV281_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8781856.1"
                     /translation="DEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKHGMRWTKYRGL
                     EKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQARVTSLEQLLST
                     V"
BASE COUNT          204 a          168 c          149 g          269 t
ORIGIN      
        1 tagttaattg tagcctatcc ataccttttc ggaagaggtg aatgacggtc tcctctaagg
       61 tatcattcgt ccgtctataa gcaggaacag tctgttgctt gaactcgccg ttgcggtcgc
      121 gcggaatctg gagatgaagt tccccgtact cggtcttgac cgtacggtca taggagccgt
      181 tacgagaatt acccgtgtta aaaccaatgc gatcgtactt ttcgtaatcg aggaaagccg
      241 ttaactccgt tttgagaagc gtattgacag ctttttccaa atggacacgg aataattcat
      301 tcaaatcgcc tttgttcgct agagtcttta gaatttctgt agtaaaatcg ttcatatgga
      361 agtcctcttt tctgtgaatg tgttgtggta actctattct acagaaggga cttccttttt
      421 tctatttaca caaaatattt tacactctcg aaaagtattg cattttatta tataaaattc
      481 agactgtaga caaaagctgt tccaaactcg taactcgagc ttggaacagc tttttatcta
      541 cttcgtttcg aatttttgaa caaaaaaata aaggctcctt gcctttccat agccaggtgg
      601 ctaatttctt taaattcatt gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca
      661 agcctcgata cttggtccaa cgcatcccat gcttttcttt tgcatccgca aatattcgtt
      721 caatcgtttg tttccgtttt ttgtaaattg atttattcag atctgttagg cgaagatgtt
      781 ccacttcatc
//
LOCUS       DACDIH010000242          784 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.251, whole genome shotgun sequence.
ACCESSION   DACDIH010000242 DACDIH010000000
VERSION     DACDIH010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.251"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(22..618)
                     /locus_tag="IV281_002945"
     CDS             complement(22..618)
                     /locus_tag="IV281_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8781857.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          298 a          147 c          119 g          220 t
ORIGIN      
        1 actaatttta caaaataaat attatccaag aagaccactt agatattgac ctccataaat
       61 caaggccata ctatataaga aaatcgacag tggtttagca atcaaaatga ttagtgtata
      121 tcgtttcact gacatgtttg tcaatccagc catcaaacat aacgcatcgt caggagcaac
      181 tggaagaaaa atagctagag caaacagctt ctcaaaacgg ttttgattat ccagccagcc
      241 aatatatttg ttatatgtct tatcgctaat catggaaagt atgaatggct ttccataacg
      301 acgccctagc agaaaaatga taatcgaacc aatgcagatt ccaatatagt tatagataaa
      361 tccagcataa gggccaaata tcaggacacc agctgcagta ctgatccctc caggaatgat
      421 tggaataact acttgcagga tttggatgaa gacaaaaata atcggtccta aaatgatgga
      481 atcacctact aatcctctta acgcattcaa atctttgaaa acacctaaat taataaaata
      541 aatcgtcagc gcgattgata ggcctaatcc gataatggat atgaaattta tcagttttcg
      601 agaggcagca atattcatta ttcatcaact ccttagaggt tctttgttaa ctatacttta
      661 ctagaaaagc aactaactgc gccatttttt gatatgacag aaaacaaaca attttgtaag
      721 ttcatcataa gtataaaaga ttgatctcaa aaaaacatcc aactaaagac ttatttaata
      781 actt
//
LOCUS       DACDIH010000243          779 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.252, whole genome shotgun sequence.
ACCESSION   DACDIH010000243 DACDIH010000000
VERSION     DACDIH010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.252"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..193
                     /locus_tag="IV281_002946"
     CDS             <1..193
                     /locus_tag="IV281_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8781858.1"
                     /translation="RIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKKDI
                     KLRVEDSTLIEQIFNDITK"
     gene            211..>779
                     /locus_tag="IV281_002947"
     CDS             211..>779
                     /locus_tag="IV281_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP8781859.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT          253 a           97 c          155 g          274 t
ORIGIN      
        1 aaggattgct gaccttatta aagacttaaa agaaaagggt gcagtagtta tagccactca
       61 tgatccggaa tttctttata aaacatgcaa tagaattatt tatttaaaaa atggtggaat
      121 aaaaaaagat ataaaactta gagtagaaga ttcaactttg atagaacaga tttttaatga
      181 cataacgaaa tagaattgag gagaatatat atgaaaacaa aagattatat ttttattggt
      241 ttagctacag ttatttccct agtaatttac tttttttcaa taatggttag tagtattggt
      301 ggggcgtttg gtcacagcat atccccaggg atttttggat tattatctgg aatcatattt
      361 gtttatatta gctataacta ccctagaaaa ggaattttta ctatttacac aattgtttta
      421 ttgttatttt ttacgttaat gggcggggcg tacttacctt ggtttatcag ttcgattagt
      481 atggcgatct tagctgatat tattctatct gtatttggat atgatcgagc aattcctcaa
      541 gttgcgtctt gggctctaat gcaattaggc agtgctgccg gacagtggat tcctatatgg
      601 ttctttacag atcgttttcg tcaagattgg atagataaag gacaatctgc tgcaacaatg
      661 gatgcaatga tccactatgc ggtaggtata tggggaatca tttcagtgtt agttgtagca
      721 agtttatcta tgattggtgt attgattgga agaaaagtct taaaaaaata caaaaaata
//
LOCUS       DACDIH010000244          777 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.253, whole genome shotgun sequence.
ACCESSION   DACDIH010000244 DACDIH010000000
VERSION     DACDIH010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.253"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..159
                     /locus_tag="IV281_002948"
     CDS             <1..159
                     /locus_tag="IV281_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP8781860.1"
                     /translation="NVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGK
                     RIIKKNHR"
     gene            125..475
                     /locus_tag="IV281_002949"
     CDS             125..475
                     /locus_tag="IV281_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781861.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(567..>777)
                     /locus_tag="IV281_002950"
     CDS             complement(567..>777)
                     /locus_tag="IV281_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP8781862.1"
                     /translation="VTGGEGWYQEEGKPARRLMAGDVVVTHKGIKHWHGATKDSWFEH
                     LAITAGNSEFLEPVTDEVYEGLENQ"
BASE COUNT          233 a          164 c          138 g          242 t
ORIGIN      
        1 aacgtcagca ctttgcacgg aggactaaat ctatataaca gtggtttttc aactggtttc
       61 gttgcaggta tccttgtacc gatactagat aactttaccg ctgtcagaaa ggaaaaaaag
      121 acacttggaa aaagaattat caaaaagaat catcgatgag cttcttactt atttatattc
      181 ccatgaaata actgaaatac gtattggagt agaatttgct gaggaagggc ttttcataga
      241 gatacagggt acggcagaaa ctcagccaga agatttagat gtacttgctg atttgctgaa
      301 caatcctcgg gaccctagta tggaaatcta ctatcaagaa ctattaggta atccccgaca
      361 tgatcgggaa gattatcatc tgttaggtag tttgattgat gacgtggaaa tcatgtatgc
      421 ccagccgatt atcaatatta agatctttcg acgtgcttct cattcctatt attaacccta
      481 ttttctattt ataaaagact gaatcaaaaa agcagctcct gaaagttcga ttaaaatcta
      541 ctttcaggag ctgttcaagc ttgacattat tgattttcca atccttcata cacttcatct
      601 gttacaggtt ctaaaaactc tgaattacca gcagtgatcg ccaaatgttc aaaccaactg
      661 tcttttgtcg caccatgcca atgtttaatc cctttgtgcg ttacgacgac atctccagcc
      721 attaatcgtc ttgctggctt tccttcttcc tgataccagc cttctcctcc ggtcaca
//
LOCUS       DACDIH010000245          762 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.254, whole genome shotgun sequence.
ACCESSION   DACDIH010000245 DACDIH010000000
VERSION     DACDIH010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.254"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            91..420
                     /locus_tag="IV281_002951"
     CDS             91..420
                     /locus_tag="IV281_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8781863.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            410..696
                     /locus_tag="IV281_002952"
                     /pseudo
     CDS             410..696
                     /locus_tag="IV281_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          285 a           89 c          145 g          243 t
ORIGIN      
        1 acgagtcaca gtaattactt gtaaatacat tatctcgtgg tataatgtat ttactaagta
       61 attacatact gttagaaatg agggatttaa atgattacaa aaacaagaaa acaaggcaat
      121 tcaatcatgt tgacagtacc taaagatttt aatgttccta atggagtcga agttgaagca
      181 aagttggtag agaatggtat tctttatgaa tttgttgaac cacaaaaaga attttatgat
      241 tttagcgaag atattttgtc tgatataatt gcagaaggct acgataaaga tgagattctt
      301 gtagaattta agaatagaaa aaacaaaatg cattcttcct ttagagatat tgctgaagat
      361 acacttacta attctaaagt aatgactaaa gaggagttgg caacagaaat tggcttatga
      421 aatcgttcct tcaaaacatg tcattaagta tttaaaaagc taaaagagaa gccattgaaa
      481 gaaaaatttt tgaatttaat ttatgatgaa attgcgattg atccatataa gggtgaacaa
      541 aaaactgggg atttatcagg tatttggtct agtgggttca aatatgcggg cacaacatat
      601 agagtagctt acgagattat agaaaacaag gttattccag tattgttatg tggaacgcat
      661 gaaaactttt atgaacagtt gaaaaaatta atctagtgat ttgttgtcgt tcttaagaag
      721 gacggcttct tttttatcga aaatctgatt cgtctacatg ac
//
LOCUS       DACDIH010000246          721 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.257, whole genome shotgun sequence.
ACCESSION   DACDIH010000246 DACDIH010000000
VERSION     DACDIH010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.257"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            194..424
                     /locus_tag="IV281_002953"
     CDS             194..424
                     /locus_tag="IV281_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781864.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            430..>721
                     /locus_tag="IV281_002954"
     CDS             430..>721
                     /locus_tag="IV281_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781865.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERY"
BASE COUNT          269 a          111 c          111 g          230 t
ORIGIN      
        1 acaaaaccac tatattctat tatgtatcta acaccctcaa aacagctata aatcaacaat
       61 accgacataa aataaacact atgttcaatc tgagatatat caaaggtaaa cgcatttttg
      121 cccccttttt gcccctcaaa tatcgcttta caacaagaac gtaagttcgt ataatcgttt
      181 tgaggtgatt tttatgatgg aagaatttat tagaaaaaat atcagtgatg aatatgcaga
      241 cttttatgat caaagcagta aaaaagataa attccagatg gatgtttcaa tcttagctat
      301 attagctttt tccgaaaata aacaacctgt aactgcaaaa aaagaaacag tattctctga
      361 aggcaaaata aaaactcgat atatattaga ggtagaaact aattttaaaa acagatcgga
      421 gtaatggtta tgctttttaa tgagacacaa ttatggttta aatttgaccc ttcgaataga
      481 tttatcaaag atttttataa agtatggaat tcagaagttt tctttttagc aatcgaagat
      541 agcttattaa ttaatctcta ttattctaat aagaactatt tcaaaattcc tgctgcgaaa
      601 actagaatga agaagggtgt atactttttg tttgatatcg tgactgacgt gccggacgca
      661 cgtagcgatc atcggcgtta tgactatata aagtatactt tcgttgatcc agaaagatac
      721 a
//
LOCUS       DACDIH010000247          718 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.258, whole genome shotgun sequence.
ACCESSION   DACDIH010000247 DACDIH010000000
VERSION     DACDIH010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.258"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..512
                     /locus_tag="IV281_002955"
     CDS             <1..512
                     /locus_tag="IV281_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8781866.1"
                     /translation="LNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKTNLE
                     NQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQSTINS
                     LNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTANKAQ
                     QIVNEHTIQ"
BASE COUNT          323 a           94 c           99 g          202 t
ORIGIN      
        1 ttttaaattc acaattgtct tcaaacaacc aaactcttga acaactaaga actaatattg
       61 aagtttccaa acaaaaagtg gcatctttag aaaacgacaa gaatcaatta gtatcagaga
      121 aaactaattt agaaaatcaa ttgtcagcaa aagactctga attgcaagga aaacaaaatg
      181 aaattaatgc gaaaatagac gaaattaacc aaaaagtagc tgaaataaat cgattaaata
      241 gcgattggaa taatcgttta caaaatgaaa agaacaaatt taatgaagaa caatctacta
      301 ttaatagttt aaaccagcaa attaatcaaa tgttgagtga aaaaaataac attcaaaatc
      361 aactgcaagc tactcagcaa aagctagaag aagaaaagtc aaaaaatagt agtttgaata
      421 attacattga aaagcttcaa aaagctaaaa atgatgtaga ggataccgca aataaagcac
      481 aacaaattgt aaatgaacat acaatccaat agatatagtt ttaatttaac aaatggtatg
      541 tttggagata atgagataga atataaatgt cataatatta tacaatctaa acaccgtttc
      601 cttttctatt tttgatgaca tcagtatata gctactcgaa agagtagtct ttttattatt
      661 ctcgcttatt attgatatga aaatttcgat ttgacaaaag gtaccgaata attttgta
//
LOCUS       DACDIH010000248          716 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.259, whole genome shotgun sequence.
ACCESSION   DACDIH010000248 DACDIH010000000
VERSION     DACDIH010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.259"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..430
                     /locus_tag="IV281_002956"
     CDS             <1..430
                     /locus_tag="IV281_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WHG domain-containing protein"
                     /protein_id="HAP8781867.1"
                     /translation="FKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFA
                     KKHHSLYMALYIDEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAV
                     TGLASLMSSNIMHPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            575..662
                     /locus_tag="IV281_002957"
     tRNA            575..662
                     /locus_tag="IV281_002957"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:607..609,aa:Ser,seq:cga)
BASE COUNT          243 a          119 c          143 g          211 t
ORIGIN      
        1 attcaaaaac atggaagatt taaaaaatac gttgctggat tctatctttg aacaactaaa
       61 agaagatgtt ttccctattg aacgtactgg tgataaaatc attgatctcg gtttgaacta
      121 tatctacttt gcaaaaaagc atcatagtct ttacatggca ttgtacattg atgaacatgg
      181 ggacggaaag aaaatgcatg atttttcata caattatttc cgtgaaatag ttaaagaaga
      241 tgaaagatac aatcaattat cggatgaata tgtcgatgct ctgcatactg gtacatgggt
      301 agcagtgact ggcttagcgt cattgatgtc atcaaacatc atgcatccaa cagaagaaca
      361 aatcattgca ctgatcaaac ggacaattga ctcgatttta gcattagata atcctgaaat
      421 aatcagatag tgttctatca gtatgaattt gatcggtaag tccgttggct gcaaactgtc
      481 aatggacttt tttgctaaaa aaagcgattt ctgttattta tctattagaa ataggcttga
      541 catttcgtga aaaaagttta aaaatagtac ttacggaagg ttggcagagt ggtaatgcac
      601 cggactcgaa atccggcgaa ccggctaata ccggcgcgca ggttcaaatc ctgtaccttc
      661 cttatatcaa cgattagagg ttgtgaagaa gctgtcctgc atcaagtaat aagaac
//
LOCUS       DACDIH010000249          713 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.260, whole genome shotgun sequence.
ACCESSION   DACDIH010000249 DACDIH010000000
VERSION     DACDIH010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.260"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..330
                     /locus_tag="IV281_002958"
     CDS             <1..330
                     /locus_tag="IV281_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781868.1"
                     /translation="EHFGLKLFQDSVGEDELPDDFNYFILETGEIEMITEPKYSVGQN
                     LYLTFYSENREDLTGDSLDIISLIQNRSIRFQRMDPNHLKLENQDRYIDQLVFTFRRL
                     LKSDCHG"
     gene            323..>713
                     /locus_tag="IV281_002959"
     CDS             323..>713
                     /locus_tag="IV281_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781869.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFD"
BASE COUNT          251 a          115 c          143 g          204 t
ORIGIN      
        1 gagcattttg gcctcaaact ctttcaagac tccgtaggcg aggatgagct acctgatgat
       61 tttaattact tcattctcga aacaggagaa atagaaatga tcactgagcc aaaatatagc
      121 gtgggtcaaa atctatatct aactttctat tcagaaaata gagaagattt aacaggagac
      181 tcactagata ttatttcatt gattcaaaat cgttcgattc gttttcagag aatggatccc
      241 aatcatttaa aactagagaa ccaagatcgc tatatcgatc aattggtatt tacgtttaga
      301 cgattattga agagtgattg tcatggctaa aaatagttgg gagctaaaaa taaatggaca
      361 tgatgaactt cttgtgcgga tggaacgcta ttcaagcgag agcgaacgac tgattaacga
      421 agcattgaaa tcaaaaggtt cggctattgc agtggatagg attacagaaa aaattcctgt
      481 ttctgaagca gatttaagaa gagggcacca acacgcaaaa aatagtcgtc cacttaagac
      541 tcaatacatt aatttgggtt tcatcattag acctacaaga aaatttgagt atttaaaata
      601 tcctgatttg gggataggta cttctaaaag aaatcagccg gacgaattta tgagaagagg
      661 attaggtctt gcacttgatc caattacaga acttctgatt cgtcaattcg ata
//
LOCUS       DACDIH010000250          709 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.261, whole genome shotgun sequence.
ACCESSION   DACDIH010000250 DACDIH010000000
VERSION     DACDIH010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.261"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>709
                     /locus_tag="IV281_002960"
     CDS             <1..>709
                     /locus_tag="IV281_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8781870.1"
                     /translation="SAELDKKDEDGKALAGTVFELRKDDGSDQVVGTYKTDSTGKLTV
                     DGLELGDYYFLETDLGTDANKTTYLLDGKKFKFSVKRGETAKVVLDVTNYKRPVITKV
                     IDKETVDTGEDVQYTLKPSIPGNIGNYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYS
                     IERDGQTTNYVFTESGMDKLQKQFDAGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYN
                     NGYEPEDETPVDHAYTII"
BASE COUNT          257 a          111 c          144 g          197 t
ORIGIN      
        1 ctcagcagag cttgataaaa aagacgaaga tggaaaggct cttgccggaa ctgtttttga
       61 attgcgaaaa gatgatggat ctgatcaggt tgttggaact tataaaacag acagcacagg
      121 taaattaact gtagatggtt tggaattagg tgactattat ttcctagaaa ccgacttggg
      181 aacagatgct aataaaacaa cctatttact agatggtaaa aaattcaaat ttagcgttaa
      241 acgtggggag actgctaaag tagttcttga tgttaccaac tacaaacgac ctgtaattac
      301 taaggtaatt gataaagaaa ccgttgatac tggcgaagat gttcaatata cattgaaacc
      361 atcaattcct ggtaatattg gtaattacga aaaatatgtt gtcaaagatg ttctaggtgc
      421 agagttaact tatgattcat cagaagtgat cgaaggaatg actttagata ctgattacag
      481 tatcgaacgt gacggacaaa cgacaaacta tgtctttact gaatcaggta tggataagct
      541 tcagaaacaa tttgatgcag gggatcgctt ctttacaatt ttgatgaacg ctcatactaa
      601 tagtaaagct aaaatggata caccacttaa gaatgatgtt tcaacgatct acaacaatgg
      661 atatgaaccc gaagacgaaa caccagttga tcacgcttat acaatcatt
//
LOCUS       DACDIH010000251          697 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.262, whole genome shotgun sequence.
ACCESSION   DACDIH010000251 DACDIH010000000
VERSION     DACDIH010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.262"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(54..629)
                     /locus_tag="IV281_002961"
     CDS             complement(54..629)
                     /locus_tag="IV281_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8781871.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          125 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaacg
//
LOCUS       DACDIH010000252          692 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.263, whole genome shotgun sequence.
ACCESSION   DACDIH010000252 DACDIH010000000
VERSION     DACDIH010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.263"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..605)
                     /locus_tag="IV281_002962"
     CDS             complement(<1..605)
                     /locus_tag="IV281_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781872.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDEL"
BASE COUNT          224 a          134 c           99 g          235 t
ORIGIN      
        1 atcaattcgt catatcttcc aaaatcaaca ttgtactcat aaccaagaag tgtcggaatc
       61 aggtcaattt caatttcttt ttcaattgaa cgtttaataa gtttcgtgga tacatacccg
      121 acaaaaaaga gaataatacc cacttcaaac aaatacatgt gtccatttac aaagtgaata
      181 aacgtcaata aaaaatcctt cactcctttt gttgttcttc cagattgttc aatttgtttt
      241 attaaagtcg ctagaattgc atttacaaca attaaatcga ataggctttt gaaaagtttt
      301 cctgctgtta atttttgcct ggtaaattgc atgactaatt gagtaaaaat agacaatagc
      361 atgacaaaaa tcaacgtcat tagcactgta aaaatgtctg atgctgcaat cctattggga
      421 tcatttatca agtaataagc cgttccaaat actccaatcg agtaaacggt taaatcaaga
      481 ataagtcgtg ccccgtaact acaaagtttt cctaatggcc ataattgcaa tacccaaaat
      541 ttctttcgtt tgctatttct tttcagagga agccaggtgt gttcaatcca gtcgtccgat
      601 ttcatttcga tttcttccct aaattctttc ctttaacgtt ttcctttgct actttagcag
      661 cgataggttg ctcaatgaac caatttttta ag
//
LOCUS       DACDIH010000253          684 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.264, whole genome shotgun sequence.
ACCESSION   DACDIH010000253 DACDIH010000000
VERSION     DACDIH010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.264"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(14..400)
                     /locus_tag="IV281_002963"
     CDS             complement(14..400)
                     /locus_tag="IV281_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781873.1"
                     /translation="MKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRFEVLKIQKNE
                     LLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTDVVMITDIYV
                     EDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          214 a          129 c           81 g          260 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg ttcctttatt tttattaaca agttttatca
       61 caggcttcat atatttgatt atatcttcat cttctacata gatatctgtt atcattacga
      121 catctgtact atttcgattg agtactttca cttgatctcc agcattgatt ttccctctaa
      181 atcttttagg aatgaaccaa ctatacatat gattagcatc accaaaatgt gcttttatcg
      241 cattctcagg atataactta ttcaatagtt cattcttttg aattttcaaa acttcaaatc
      301 tatacttatc atcttctgct aatctaaata aatcttctaa acgcatgttt tctaactgta
      361 agttttgaaa atcttccggt ataaattttc tatatttcat taataatgaa cgcccagccc
      421 gatttattag aatctggcca ttttggtcat ctctataaag aattccgtaa cccatcaacg
      481 ccaatagcac acgtgacgtt ttgttacttg taatattcat atttccttga ttacctgagg
      541 caatgaaaga cttatctact tgaattaggg tgaaattctc attcaaatat ggtagtaaaa
      601 ttaatatatg tgaaaatcga atcttgagat agctattccc cttgagatat tcactgatac
      661 ttctactcaa atcatttaaa tttt
//
LOCUS       DACDIH010000254          681 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.265, whole genome shotgun sequence.
ACCESSION   DACDIH010000254 DACDIH010000000
VERSION     DACDIH010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.265"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(44..>681)
                     /locus_tag="IV281_002964"
     CDS             complement(44..>681)
                     /locus_tag="IV281_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAP8781874.1"
                     /translation="RGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTD
                     LTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTD
                     RGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQL
                     RLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          191 a          161 c          124 g          205 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttgac agatttatag gttgattcca caacggcatt atcatacgga caacccttcc
      301 tactcaatga acgagtaatt ccaaaaccat tcagaatctc atgaatggtt tggttatcaa
      361 attcctttcc ccggtctgta tgaaaaagct tgacgtctgt taaagaatac ggtatccgtc
      421 caaaggcttc ttttaccaat gaggcatctt tcttctcacc acaggaataa ccaataattt
      481 ctcgattaaa caaatcaagt attaagcaga tataatgcca ctttttcccc acgcgaacat
      541 aagtaagatc cgtaacgatg gcttccaatg gctgttcttg tgtaaaggtc cgatctaata
      601 cattcgctgt tttcgcttca ttacaagctg ttcgttgccc tttaaaatga gcgatcgtat
      661 aggtagatgt caatccgcgg c
//
LOCUS       DACDIH010000255          670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.268, whole genome shotgun sequence.
ACCESSION   DACDIH010000255 DACDIH010000000
VERSION     DACDIH010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.268"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            154..417
                     /locus_tag="IV281_002965"
     CDS             154..417
                     /locus_tag="IV281_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8781875.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            417..>670
                     /locus_tag="IV281_002966"
     CDS             417..>670
                     /locus_tag="IV281_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8781876.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
BASE COUNT          253 a           88 c          140 g          189 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg
       61 ctgttttggt actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt
      121 agaatggaga tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc
      181 tcagcacgga aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt
      241 gaaatcatta gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa
      301 gcaatcaaag aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga
      361 gaaaaagatg atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga
      421 ataactattc tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt
      481 ctaatttgaa acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc
      541 ctacccaatc atttgagaag ttaaaaccta cacatgaagg taggtattct agaagattga
      601 atagacaaca ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt
      661 cagcaaggac
//
LOCUS       DACDIH010000256          668 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.270, whole genome shotgun sequence.
ACCESSION   DACDIH010000256 DACDIH010000000
VERSION     DACDIH010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.270"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>668
                     /locus_tag="IV281_002967"
     CDS             <1..>668
                     /locus_tag="IV281_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP8781877.1"
                     /translation="WFGFTGTPIFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALED
                     GSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLD
                     PAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEM
                     TKEPDWLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYN
                     QYYD"
BASE COUNT          244 a          122 c          135 g          167 t
ORIGIN      
        1 ttggtttggc tttacaggta caccgatttt tcctgagaat cggaaacaag ccaaagggca
       61 attggcacgt acaacccatg atcagtatgg agaggtcttg catacctata ccattaagaa
      121 tgcgctagaa gatggctctg tcttaggatt tcaagtagag catgaaaaca ccgtggagcc
      181 gacttcacta gagaataaaa tctatcggaa attaaaagaa gtagaaacat atgcagagta
      241 ttcatcagaa cagatcaatc gtatgattga tcaaatggaa cctgtcaaaa aagaaagtta
      301 tttggaccca gctgtttttg aagcagatga acatatccaa aaagtcattc ataaaatgtt
      361 ccgtccagat aatgcctata caaaatttga ttttcgaaat ggcaggccaa cgaaatctgc
      421 aatcttaacc acgagttcca ttgacatggc caaaaagtat tatcgggcca tcaaagagat
      481 gacgaaagag cctgattggt taacaaaaga gtttcccaat cagctcattc gtgaaggacg
      541 aacgatggag gatcctgatt ttccacgaat cgctattacc tattcattag aagaaaatag
      601 ccgagattca agtgcccaac aagaagaaat gcaaaaaatt attgaggaat ataatcagta
      661 ttatgata
//
LOCUS       DACDIH010000257          652 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.272, whole genome shotgun sequence.
ACCESSION   DACDIH010000257 DACDIH010000000
VERSION     DACDIH010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.272"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..213
                     /locus_tag="IV281_002968"
     CDS             <1..213
                     /locus_tag="IV281_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HAP8781878.1"
                     /translation="SKNIAVWFFFGAVVMAYLELPLIAIAAVGTIIAIVIAQRDLQLK
                     NISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            210..>652
                     /locus_tag="IV281_002969"
     CDS             210..>652
                     /locus_tag="IV281_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004842325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8781879.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLV"
BASE COUNT          173 a          114 c          168 g          197 t
ORIGIN      
        1 agtaaaaata ttgctgtgtg gtttttcttt ggggccgttg ttatggcgta tctcgagtta
       61 ccattgattg caattgccgc agttggaacg attattgcaa ttgttatagc ccaaagagat
      121 ctacaactaa aaaatatatc tgttcatgca gttcaaagcg atgctgctgt aaatgcaacg
      181 cttgaccaag aagaggagga tttctttcaa tgaaattagc tgatggaatt tctaaagatg
      241 agaaaaaatt ggttcggaag atgttttggc gctcgatgac tatgtatgct tcggtgaatc
      301 cggtgacgat gggcggcggt ggtttcgcct attctatgat gcccttcatt aatcatttct
      361 acaaaagtga agaagatcga gctgcggcgc tggaacgtca tacacagtat ttttcgacga
      421 ccattccttg cgcgtccttt gtcatgggga ttgcggcatc gatggagaag gaaaattctg
      481 aaaaagctgg ttttgacgtg gaaagtatca acgcgatcaa aacgagtttg atgggaccat
      541 tggctggaat tggcgattca ttgttctggg gagtttggcg tgtcgttacc gcgggactag
      601 cgatcaactt agccaatgcc ggaaatattt tggcaccaat tatcttttta gt
//
LOCUS       DACDIH010000258          622 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.274, whole genome shotgun sequence.
ACCESSION   DACDIH010000258 DACDIH010000000
VERSION     DACDIH010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.274"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>622
                     /locus_tag="IV281_002970"
     CDS             <1..>622
                     /locus_tag="IV281_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAP8781880.1"
                     /translation="EMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQ
                     NRLLSEKNYPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEE
                     TWTTFFEYLKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPK
                     KNSKSFREAVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLD"
BASE COUNT          221 a          112 c          115 g          174 t
ORIGIN      
        1 ggaaatgtat gtatcaggcg tttcaactcg taaagtatca aaaattgtgg aagaactttg
       61 tggtaaatcc gtctctaagt ccttcgtttc tagcttaaca gaacagctag aacctatggt
      121 taacgagtgg cagaatcgtt tattatcaga aaaaaattat ccttacttaa tgaccgatgt
      181 actctatata aaagtacgag aagaaaatcg agtactctca aaaagctgtc atatagcgat
      241 tggaataacc aaagatggcg accgtgaaat tatcggcttc atgattcaaa gtggcgaaag
      301 cgaagagacc tggacaacat tttttgaata cctaaaagaa cgcggtttac aaggtacgga
      361 actcgttatt tctgatgcgc acaaaggatt agtctctgcc attagaaaat ccttcaccaa
      421 cgtaagttgg caaagatgcc aagttcactt cctaagaaat atctttacca ccattcctaa
      481 aaaaaattca aaatctttca gagaagctgt taaaggaatt tttaagttca cagatattaa
      541 cttagcgcgt gaggctaaaa atcgattgat tcatgattat atcgatcaac caaaatattc
      601 aaaagcttgc gcatcattgg at
//
LOCUS       DACDIH010000259          617 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.275, whole genome shotgun sequence.
ACCESSION   DACDIH010000259 DACDIH010000000
VERSION     DACDIH010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.275"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..614)
                     /locus_tag="IV281_002971"
     CDS             complement(<1..614)
                     /locus_tag="IV281_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014096946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP8781881.1"
                     /translation="MPFQTSAKITIENLHPKDMSSFFYTINYALVDELEENSLYFHAN
                     WHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLERYWWGEGEFKFYIDGDSEYPTV
                     TSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYPYQSNRDATRDRFETGKLNAVH
                     AFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGNDDVRLFER"
BASE COUNT          177 a          140 c          122 g          178 t
ORIGIN      
        1 tgccgctcaa ataagcgaac atcatcgttg ccgatttgtt gtaaagtgac cgataaatct
       61 tcggtaaaac agatggggtc ttgaagatgc caacgataaa gaccatgtcg tggcattgcg
      121 tcgtcaccaa aggcatgaac ggcattcaat tttcccgttt caaaacgatc tcttgtggca
      181 tctctatttg attggtaagg ataccctaaa aaggctgttg aatagttttt cgcatgtgga
      241 cgtccgaact catcgtactc atgaaaggcc cacgccccgc caaaataatc ttctgaacct
      301 gttgaggtta cggtcggata ctcactatcc ccatcaatgt agaatttaaa ctcgccttcg
      361 ccccaccaat aacgttctag gcaggtaagc gcaaggtaag tacccacgta atagccttga
      421 ccctttaatc gatcaattaa cttataatcc tcctgtaatt tggtaatgcg ttcccgatgc
      481 caatttgcgt gaaaatatag gctattttct tccaattcgt ccaccaaagc gtaatttatc
      541 gtataaaaaa atgaggacat atccttcgga tgtaaattct cgatcgtaat tttagcggat
      601 gtttggaaag gcatttc
//
LOCUS       DACDIH010000260          616 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.276, whole genome shotgun sequence.
ACCESSION   DACDIH010000260 DACDIH010000000
VERSION     DACDIH010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.276"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            152..>616
                     /locus_tag="IV281_002972"
     CDS             152..>616
                     /locus_tag="IV281_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781882.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKD"
BASE COUNT          213 a           69 c          108 g          226 t
ORIGIN      
        1 tttttttatt tcatcatcta atataaacat ataaatctcc tttttttatt tgtatgacaa
       61 gtttaccata aaaaaaaatt aaaaagcgag acatatttat gttgttgtgt tatcataata
      121 acataaatat gtagagtgag atgatgaaaa aatggatttg aaacaatcgt tttacatctg
      181 gttatatcct gataatccta tgtcaataat ggctgatatt tttgtagtta gcatcttatt
      241 gtatttattt tatctaattt tctttcacaa tttaatcggg tacatgattc ttgattggaa
      301 aggaatgttc gatatagaaa gaaaaccatt tgctaacaat caaacctata ccgtcttcga
      361 aagtggagat tgggaagaaa atgaagttga tgtgatccat gaaattggat tagctttatc
      421 aggaatacaa gatgctgatg ttttggtatc aatgacaacg gatactggat acaacgctta
      481 tgaggtattt tatcttatgt attttatatg gcgtgatggt caagaaatta tcaatggtcg
      541 tgctgaaaaa acaatcaatt ctaatactga aatttctttt tgtatgggtg aaggggaaat
      601 gattgtatat aaagat
//
LOCUS       DACDIH010000261          614 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.277, whole genome shotgun sequence.
ACCESSION   DACDIH010000261 DACDIH010000000
VERSION     DACDIH010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.277"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            107..>614
                     /locus_tag="IV281_002973"
     CDS             107..>614
                     /locus_tag="IV281_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP8781883.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPL"
BASE COUNT          233 a          110 c           98 g          173 t
ORIGIN      
        1 agagtataaa atattttgtg taaatgaaaa aatccataca aaaaaggaag tcccttctgt
       61 agaataaagt taacgacaac caattcacag aaaagaggac ttccctatga atgattttac
      121 tacagaaatt gtgcaaactc tagtcactaa aggcgattta aatgaattat tccgttcgca
      181 cttagaaaaa gcgataaaca cactcctacg gactgaatta acggcttttt tagattacga
      241 aaaatatgat cgcactggtt ttaattcagg taattcgaga aacggttctt actttcgatc
      301 aatcaaaacc gaatatggtg aattaacatt ggaaatacct agagatcgta atggtgagtt
      361 taaacaacaa actttaccag cctacaaaag aacaaacgat acattggaaa ccactattat
      421 ccatttattc gaaaaaggtg ttacgatgtc tgaaattgct gatttgatcg aaaaaatgta
      481 cggtcatcac tatactccac aaaccatgtc caacatgact aaagttctga ctgaagaagt
      541 aaatgccttt aaatccagag ccttaaatga taagtatgtc gctattttta tggacgctac
      601 ttacattcca ctaa
//
LOCUS       DACDIH010000262          604 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.278, whole genome shotgun sequence.
ACCESSION   DACDIH010000262 DACDIH010000000
VERSION     DACDIH010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.278"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..359)
                     /locus_tag="IV281_002974"
     CDS             complement(<1..359)
                     /locus_tag="IV281_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8781884.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITS"
BASE COUNT          206 a          103 c           79 g          216 t
ORIGIN      
        1 gatgtaattt tttcaacaat atcttgatat ttggttccag ctgattggaa taaaaccaaa
       61 tcgaatatac gtttattctc aaaaatataa ttaataaatg gatctaattc attgaagttc
      121 tctctccaac ttttctctat atctatattt tctaagttta agaggtattt ggtaaactct
      181 tcattataca tattaataag cgcattaata tgcggctgaa taatttcttc aaataacgct
      241 tccttagaag taaaatgttt ataaaaagca cccgtagtta cgcctgcggt caagcacagt
      301 tgccttatct tagtttgctg aaatcctact ttttcaaaat ggcttagtcc actttctatt
      361 aatttttggt gagttgtaat ataatctctg gtttctatgg gtacttcctc ctaataagat
      421 gaattaacta tattatctta cacaggactt gaagtttcaa tcgttaacgt ctattactga
      481 actcattcct tagagctaaa ctaatgagaa agagcaaaat aactgataca aaaagcaatc
      541 ctacaagaat taatagactc atctaaatca ttcacctttt ctaaaataat ccgagaaaga
      601 ttta
//
LOCUS       DACDIH010000263          576 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.281, whole genome shotgun sequence.
ACCESSION   DACDIH010000263 DACDIH010000000
VERSION     DACDIH010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.281"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..>576
                     /locus_tag="IV281_002975"
     rRNA            2..>576
                     /locus_tag="IV281_002975"
                     /product="23S ribosomal RNA"
BASE COUNT          184 a          114 c          166 g          112 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatcta
//
LOCUS       DACDIH010000264          556 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.283, whole genome shotgun sequence.
ACCESSION   DACDIH010000264 DACDIH010000000
VERSION     DACDIH010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.283"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(152..346)
                     /locus_tag="IV281_002976"
     CDS             complement(152..346)
                     /locus_tag="IV281_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781885.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSSDI
                     LTLNLTTKTFVDATEDYPFV"
BASE COUNT          171 a          113 c           88 g          184 t
ORIGIN      
        1 tgtgatagag aaataacaat ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat
       61 ttgattttga tcgggtctag tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc
      121 ttaaatttca tcttgactta tcaccattag actaaacaaa tggataatct tctgtcgcat
      181 cgacgaacgt cttcgtagtc agatttaacg ttaaaatatc gctactttga taagcttttg
      241 ccgctttgtc tgtgacgaca aaagatctgc ctgtttgaat ttctgtcaca aaaacgagat
      301 tgtctctgct tttttcaaca acaactccaa tcgttgaatg aaccatttct gcttcctccc
      361 ttctctttct tttgatataa tgtagtcgca aatcgcacca ctcaatgagt tggcggaaaa
      421 caattcattg aaaaaaagag tcctggacaa tcacgttcag gactctttta cttcttttat
      481 tcacttaaca actaacactc tgtgagtgaa atggactctt caatgtgatg ttaggatgat
      541 ttcatggaca cgattt
//
LOCUS       DACDIH010000265          555 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.285, whole genome shotgun sequence.
ACCESSION   DACDIH010000265 DACDIH010000000
VERSION     DACDIH010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.285"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            73..>555
                     /locus_tag="IV281_002977"
     CDS             73..>555
                     /locus_tag="IV281_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781886.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     G"
BASE COUNT          201 a           79 c          116 g          159 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccgagaa ccttgggttt tcaagtgctt tttggttcat taatcttaac catagtgttt
      121 ttaaagtgga aaacaagcaa caaaaaaagt atgtggagcc ttgtaggaat agttcttatt
      181 atttttactg tgactgcagc aacagaagca aattattcgc ataaaattca gtcgtataaa
      241 ggtcgtttag ttatgaccaa aaaagatgtt acacgttata catcctcaag aactggaggc
      301 ctatatgact atgcaccatt agatgcattg aaatataaag atcatttaaa gaaacatgaa
      361 gtaaaagttg gtgatcggtg ggagaatgca tctcataaag catataattc caaaatagtc
      421 tatacagtag aagatgccga tgagcaaaat atcacattac ctgtattcaa ttattgggga
      481 acagttgtga aagtaaatgg gaaaaagagc actatagaaa atgacaatgg tttagtgtct
      541 attaaaggta cgggg
//
LOCUS       DACDIH010000266          549 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.287, whole genome shotgun sequence.
ACCESSION   DACDIH010000266 DACDIH010000000
VERSION     DACDIH010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.287"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..390
                     /locus_tag="IV281_002978"
     CDS             <1..390
                     /locus_tag="IV281_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8781887.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          205 a           95 c           83 g          166 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caatttattt
      421 acacaaaatt attgacgctc ccatcctaaa acataagcta tttgtttact tctgtagaat
      481 atatactttg ttgttagata agttgcacat gtttatagaa tctaaataat acccgtggtg
      541 ctagttaat
//
LOCUS       DACDIH010000267          541 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.289, whole genome shotgun sequence.
ACCESSION   DACDIH010000267 DACDIH010000000
VERSION     DACDIH010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.289"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..182)
                     /locus_tag="IV281_002979"
     CDS             complement(<1..182)
                     /locus_tag="IV281_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP8781888.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNL"
     gene            437..>541
                     /locus_tag="IV281_002980"
     CDS             437..>541
                     /locus_tag="IV281_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8781889.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKK"
BASE COUNT          225 a           67 c           91 g          158 t
ORIGIN      
        1 aaaagattgc ttggcgaggt aagaatagtt agcaatccac tccaattatc agtaggagta
       61 ttagaaagca agccaataaa aagaaaaaaa acacctgtaa aaccaagata aaaatacttt
      121 cttttctgtg aaacgattcg tttttgagga tgaatataaa taacgtcagt ttcgttttcc
      181 attacacacc ttctttttca ttttctttaa ctataacttg aaatgaaaga tttggacaac
      241 tatcatttag gtcaaaacta aaaatgtaag ggaacccatt gaatttaacc aaattttttt
      301 aattttgcaa aaaaaagtta aagaagactg aaaaaaattt aaaatataac gttttgagta
      361 aaatgtttgc aaaaaatgct aaaaataggg tacaataaaa caacatatac tgcagtaaag
      421 aagtggggga gttttaatga gaagaaaagt ctataccaag gatcaaattc taaaagctgc
      481 ttatgatgtt gttgcaaagg aagggtttaa aggatttact gcgagaaata ttgctaaaaa
      541 g
//
LOCUS       DACDIH010000268          528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.291, whole genome shotgun sequence.
ACCESSION   DACDIH010000268 DACDIH010000000
VERSION     DACDIH010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.291"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(5..90)
                     /locus_tag="IV281_002981"
     tRNA            complement(5..90)
                     /locus_tag="IV281_002981"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IV281_002982"
     tRNA            complement(107..178)
                     /locus_tag="IV281_002982"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IV281_002983"
     tRNA            complement(190..262)
                     /locus_tag="IV281_002983"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IV281_002984"
     tRNA            complement(280..361)
                     /locus_tag="IV281_002984"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IV281_002985"
     tRNA            complement(369..441)
                     /locus_tag="IV281_002985"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IV281_002986"
     tRNA            complement(445..517)
                     /locus_tag="IV281_002986"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACDIH010000269          516 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.292, whole genome shotgun sequence.
ACCESSION   DACDIH010000269 DACDIH010000000
VERSION     DACDIH010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.292"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>516)
                     /locus_tag="IV281_002987"
     CDS             complement(<1..>516)
                     /locus_tag="IV281_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP8781890.1"
                     /translation="ETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESYPISVPSS
                     YNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNGKFITQHK
                     GGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKKENLVNFD
                     FFNYAGIHRPV"
BASE COUNT          159 a          107 c           63 g          187 t
ORIGIN      
        1 taacaggtct atgaatacct gcataattga agaaatcaaa attaactaag ttttcctttt
       61 ttatatcccc attttcctgt actgtttcct tcataatccc cacaggtaaa gtactatcgt
      121 caataatatt gtttacagca actgtaattc gattattccc gtcttttaca aaccgattaa
      181 tttcagactc aaatggtgta aacccacctt tgtgttgagt aataaacttt ccattcaaaa
      241 aaacttttgc ttcatgtgtt gcagatccaa aacgaagaac taatctttct tctaataatc
      301 ttttaggaag agtcacttct ttttcatacc acacccatcc aacatgattt ctaatgttct
      361 ctgtttcaaa taaatcatta taagaagagg gaactgaaat cggataactc tctttttcat
      421 ttattggttc taaagcaagt tcttcctgta agtcatcatt tgtatctaat ttaaatctcc
      481 agagaccgct taagtcaatt acttgcctcg tctctg
//
LOCUS       DACDIH010000270          515 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.293, whole genome shotgun sequence.
ACCESSION   DACDIH010000270 DACDIH010000000
VERSION     DACDIH010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.293"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IV281_002988"
     CDS             complement(<1..132)
                     /locus_tag="IV281_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8781891.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          187 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaa
//
LOCUS       DACDIH010000271          511 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.296, whole genome shotgun sequence.
ACCESSION   DACDIH010000271 DACDIH010000000
VERSION     DACDIH010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.296"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>511
                     /locus_tag="IV281_002989"
     CDS             <1..>511
                     /locus_tag="IV281_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP8781892.1"
                     /translation="QNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTFQESDIE
                     KWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNWGSYPAT
                     NRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSNSLSSAL
                     LASLYFEHTG"
BASE COUNT          146 a           93 c          114 g          158 t
ORIGIN      
        1 caaaatgtgt tgatttctca aaaaggaaat aaggacacga tcgcacaaca ggaagtggca
       61 agcttcgcca ttgcttttgt ggacccaaat gcttttgaag aagtaatgac ttttcaggaa
      121 tctgatatcg aaaaatggct gaaggaaaca ttgaactatt ttgctcagct atttgggcag
      181 ctgtcaatgc caaacgatag agttgtccat ctctatcgtc gtgcacttta tcaaagcttt
      241 tctagtttcg gtatgaatcg acagggacag gtcgtcggca gcaattgggg cagctatcct
      301 gctactaatc gaatttggaa taaggatatg tattacgcat cattgccatt caccatgttt
      361 gaaccggagt tatgtcaaaa aacggttcta tggtttgatc aatatggcgt taagtttcct
      421 ggttcaaaat ttacaggagg tatcaatcat tctctgagta attcgttatc cagtgcgtta
      481 ctggctagtc tttattttga acatactggg g
//
LOCUS       DACDIH010000272          508 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.297, whole genome shotgun sequence.
ACCESSION   DACDIH010000272 DACDIH010000000
VERSION     DACDIH010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.297"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>508
                     /locus_tag="IV281_002990"
     CDS             <1..>508
                     /locus_tag="IV281_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781893.1"
                     /translation="KFANSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLL
                     VNTVVELAKWAKSNETYSQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENS
                     QWIKELKREDFIETLRILKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEK
                     RIDNIINY"
BASE COUNT          208 a           55 c           92 g          153 t
ORIGIN      
        1 ctaaatttgc aaactcattc tggtttttaa aaaatattaa taaaacagtg gaaaagaatg
       61 gagtttataa aggtgatttt cattttcaaa atgctgagac tattgaacga gaacatcatt
      121 atttcgattt attagtgaat acagtggtag aattagcgaa atgggcaaag tcaaacgaaa
      181 cttatagtca agtacttaaa agaattatga gtcaagaaac ttcatttatc tatgagttaa
      241 gcaagtcaaa tttgaaatat acttataatg aagatgaact tgaaatttta gataatccag
      301 aaaattcaca atggattaaa gagttgaaga gagaagactt tattgagaca ctaagaattc
      361 taaaatcgtt atttgatttg aaattagcca aagctaccat catgggaatt gaaagagatt
      421 catattattg ggaggaaatt ggactatatg ctccccagat tgaaggtgaa gataaagaga
      481 aaaggataga caacattatt aactatat
//
LOCUS       DACDIH010000273          505 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.299, whole genome shotgun sequence.
ACCESSION   DACDIH010000273 DACDIH010000000
VERSION     DACDIH010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.299"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(97..>505)
                     /locus_tag="IV281_002991"
     CDS             complement(97..>505)
                     /locus_tag="IV281_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP8781894.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          159 a          100 c           78 g          168 t
ORIGIN      
        1 aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat tatgactgta
       61 tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt ttatctgttg
      121 ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt tattcatatt
      181 tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt tatcaacgtt
      241 acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc atactccttt
      301 ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag attctgaatc
      361 acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa cagaagagag
      421 atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc tgatctggtt
      481 tgaaaaatga ttttctggtg ttcca
//
LOCUS       DACDIH010000274          504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.300, whole genome shotgun sequence.
ACCESSION   DACDIH010000274 DACDIH010000000
VERSION     DACDIH010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.300"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(9..311)
                     /locus_tag="IV281_002992"
     CDS             complement(9..311)
                     /locus_tag="IV281_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781895.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          186 a           85 c           72 g          161 t
ORIGIN      
        1 ttttttattt aatctttgta tctttctgga tcaacgaaag tatactttat atagtcataa
       61 cgccgatgat cgctacgtgc gtccggcacg tcagtcacga tatcaaacaa aaagtataca
      121 cccttcttca ttctagtttt cgcagcagga attttgaaat agttcttatt agaatagtag
      181 agattgatta ataagctatc ttcgattgct aaaaagaaaa cttctgaatt ccatacttta
      241 taaaaatctt tgataaatct attcgaaggg tcaaatttaa accataattg tgtttttcct
      301 tccatcagca taaaagttca cctcaaaaag agtatacgaa cgaatgttct ttttgtgaag
      361 gcggaattta atatactgaa taatagaaaa caagtacttg tgccaaatta tgtgccaaaa
      421 aaattcgaat tcaacaaaat gtaaacaaat agaaaacctt ctatattaag atttcctgca
      481 ataacaaaca caacagaaga cgtg
//
LOCUS       DACDIH010000275          503 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.301, whole genome shotgun sequence.
ACCESSION   DACDIH010000275 DACDIH010000000
VERSION     DACDIH010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.301"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>503
                     /locus_tag="IV281_002993"
     CDS             <1..>503
                     /locus_tag="IV281_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP8781896.1"
                     /translation="DQLKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPS
                     RSRFTRLSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIA
                     KVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGD
                     KGYISHK"
BASE COUNT          167 a           92 c           91 g          153 t
ORIGIN      
        1 tgatcaactg aagcaacgcg atacagtgat tattgcttgt gttatatggg gcataattaa
       61 tggctatact agccaaagag ccacgtatag agcggtttgt tccgtcttat ttcctaatgg
      121 tgactttcct agtaggagtc gattcactcg cttaagttca aacttagctt acactatcaa
      181 gattattcga tactttttca tcaagaagct caccaaaggt gaactagtcg gaattataga
      241 tagttttcct agtccattat gtaaaccagt tagaaataga caagcaaaac tattgaatca
      301 aatagccaaa gttggctata actcaacaaa aaaatcttat ttttatggtc ttaaaattca
      361 tatgatcgtt actaagactg gctttccgat tacttactca atcacgaatc caggtgtcca
      421 tgacgtgaaa gtgttagaga ctttatcgga agaagcgaat cttcccaata tcctagggga
      481 caaaggatat attagccata aaa
//
LOCUS       DACDIH010000276          501 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.302, whole genome shotgun sequence.
ACCESSION   DACDIH010000276 DACDIH010000000
VERSION     DACDIH010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.302"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            159..>501
                     /locus_tag="IV281_002994"
     CDS             159..>501
                     /locus_tag="IV281_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781897.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIEN"
BASE COUNT          192 a           46 c           81 g          182 t
ORIGIN      
        1 caggtgatta aacttttgaa cgacttgtaa ttctatattt tttcttttaa gtaaaaaata
       61 taggtgattt tttttgactt catatttata ataaagacac gtaaatcaat tatttttcat
      121 tttactttaa ctaataatgt ttatttggag ggattattat gttagatgat agattgacgt
      181 gttctaattg tggtacatta ttttactatg aggaagttag aaatattgta aaacatagaa
      241 aaaaagaaat gcctatacgt tgtccaaatt gcaatgaagt tattaagcga aagttgtctc
      301 atggttattt tgtcagctac gaactatatg aagaaagtaa aataaatctg aaaaaaaaac
      361 caaaatacta taaaaatttg aaaactggag ctattataac tgaaaaagaa tttaaaaatt
      421 tagtgactga agaagaaagc aggttttata atttgtctgg taatatttgc cctggattta
      481 tggatcagat tattgaaaat g
//
LOCUS       DACDIH010000277          480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.303, whole genome shotgun sequence.
ACCESSION   DACDIH010000277 DACDIH010000000
VERSION     DACDIH010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.303"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..415)
                     /locus_tag="IV281_002995"
     CDS             complement(<1..415)
                     /locus_tag="IV281_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781898.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKICWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFF"
BASE COUNT          180 a           77 c           62 g          161 t
ORIGIN      
        1 aaaaaaacgg ctttccaaat gatgaaatac ctaaatctaa cttattaaga aagttgaaat
       61 ttttatattc aggaaaccat ttattaattt catgtgcagc acgtaatgca ttccctggag
      121 ttgtcaaaga gaaaattaat tctgctaagc ttattattga aaatactgat aacttataat
      181 tatatcgtcc attgacaatc aaatataaac taactatact tgttagaaca aaaaaacagg
      241 cgttgacttg ttcttgatta gttaaaaata tcaataaagg aatacttacc caacatattt
      301 ttctatttat ttcttctcct cttaagagtt gaaaaaacgg ataaaaagtt agtaaactaa
      361 atgctactgg ccactgatag ttcaaggtgg ttgctctcca accggtttcc cccattactg
      421 tcaatggtat taaacagtat atagagcaaa ccaataataa atttttaaca tttattttct
//
LOCUS       DACDIH010000278          459 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.308, whole genome shotgun sequence.
ACCESSION   DACDIH010000278 DACDIH010000000
VERSION     DACDIH010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.308"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>459
                     /locus_tag="IV281_002996"
     CDS             <1..>459
                     /locus_tag="IV281_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP8781899.1"
                     /translation="YRFDFRKVKNLDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGL
                     NFPGNADYKELQEGKDNVAPFEFIPWILGQCSTIDEAKELLATINLVNIDFSEKLPLS
                     PLHWLLADKEKSIVIESMKDGLHLYDNPVGVLTNNPPFDYQLFNLNNYRSL"
BASE COUNT          163 a           70 c           85 g          141 t
ORIGIN      
        1 tatcgtttcg attttagaaa ggtaaaaaat ttagataaac attatgcaat gataggaata
       61 gcagctgggg tatccaacta tccactctat tatgaggcta caaatgaaaa agggttgagt
      121 atggcaggac tgaattttcc tggcaatgct gattataaag aattacaaga aggaaaagat
      181 aacgttgcac catttgagtt tattccatgg atactagggc aatgctcaac catagacgaa
      241 gctaaagaat tattggctac tatcaatcta gtcaatatcg attttagtga aaaactaccc
      301 ttgtcacctt tacactggtt attagctgat aaagaaaaat cgatagtaat tgaaagtatg
      361 aaagatggac ttcatttata tgataatcct gtcggtgtgt tgacaaataa tccgccattt
      421 gattatcaat tatttaatct gaacaattat cgctcgcta
//
LOCUS       DACDIH010000279          442 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.309, whole genome shotgun sequence.
ACCESSION   DACDIH010000279 DACDIH010000000
VERSION     DACDIH010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.309"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>442)
                     /locus_tag="IV281_002997"
     CDS             complement(<1..>442)
                     /locus_tag="IV281_002997"
                     /inference="COORDINATES: protein motif:HMM:NF024832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP8781900.1"
                     /translation="YDKTYLDFIKIVSIFIIPLGIGIYVYRELVTNILLGSQWKEAVP
                     FVGIYGLMSCFTLVLGQYASEYYRGLGKPKANVLMTILHLAVLIPVLIIYSRKGFIEL
                     AYGRSFVKIEQIIVFWIILWFGFKFNPFKILKQVWKSCASALLMG"
BASE COUNT          168 a           88 c           52 g          134 t
ORIGIN      
        1 cccattagca atgcactagc acaagatttc caaacctgct ttaaaatttt aaatggatta
       61 aacttaaaac caaaccataa aataatccaa aagactataa tctgctctat cttaacgaaa
      121 gatctaccat atgctagttc tataaaccct ttccttgaat agattattag aactggaatt
      181 aatactgcta aatgtaggat agtcataaga acatttgctt ttggttttcc taaaccacga
      241 tagtactcag aggcatattg tcccaagaca agagtaaaac agctcattaa tccatatatc
      301 ccaacaaatg gtacagcctc tttccattga ctcccgagta gaatatttgt tactagttcc
      361 ctatatacat atatacctat acccagagga ataataaaaa tggaaacaat tttaataaaa
      421 tctaaatatg tcttatcata ct
//
LOCUS       DACDIH010000280          428 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.311, whole genome shotgun sequence.
ACCESSION   DACDIH010000280 DACDIH010000000
VERSION     DACDIH010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.311"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            82..>428
                     /locus_tag="IV281_002998"
     CDS             82..>428
                     /locus_tag="IV281_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP8781901.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHV"
BASE COUNT          150 a           78 c           84 g          116 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgt
//
LOCUS       DACDIH010000281          428 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.312, whole genome shotgun sequence.
ACCESSION   DACDIH010000281 DACDIH010000000
VERSION     DACDIH010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.312"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            82..>428
                     /locus_tag="IV281_002999"
     CDS             82..>428
                     /locus_tag="IV281_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP8781902.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHV"
BASE COUNT          148 a           74 c           86 g          120 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgt
//
LOCUS       DACDIH010000282          415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.314, whole genome shotgun sequence.
ACCESSION   DACDIH010000282 DACDIH010000000
VERSION     DACDIH010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.314"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>415
                     /locus_tag="IV281_003000"
     CDS             <1..>415
                     /locus_tag="IV281_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8781903.1"
                     /translation="SDADKYNPEGQKVTTELNKEPDASEGIKNKEDLPKDTKYTWKEK
                     VDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVDL
                     TDNVTNLPTLPEGTTVTDVTPDGTIDTNTPGNYEGV"
BASE COUNT          165 a           66 c           95 g           89 t
ORIGIN      
        1 atctgacgct gacaaatata atcctgaagg tcaaaaagtg actacagaat tgaataagga
       61 accagacgca tctgagggaa ttaagaacaa agaagattta ccaaaagata ctaagtatac
      121 ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa aaagggactg ttgtagtgac
      181 atatccagat ggatcatctg atgaagttga agtagatgtt acagtgacag acaatcgttc
      241 tgacgccgat aaatatgagc caacagtaga aggcgaaaaa gtagaagttg gaggcacagt
      301 agatctaaca gataatgtta ctaacttgcc aacattacca gaaggaacaa cagtaacaga
      361 tgtcactcct gatggtacga ttgataccaa cacaccaggt aattacgaag gggtt
//
LOCUS       DACDIH010000283          401 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.denovo.318, whole genome shotgun sequence.
ACCESSION   DACDIH010000283 DACDIH010000000
VERSION     DACDIH010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.denovo.318"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>401
                     /locus_tag="IV281_003001"
     rRNA            <1..>401
                     /locus_tag="IV281_003001"
                     /product="23S ribosomal RNA"
BASE COUNT          125 a           87 c          117 g           72 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt c
//
LOCUS       DACDIH010000284         7321 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.guided.104, whole genome shotgun sequence.
ACCESSION   DACDIH010000284 DACDIH010000000
VERSION     DACDIH010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.guided.104"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1..1341)
                     /locus_tag="IV281_003002"
     CDS             complement(1..1341)
                     /locus_tag="IV281_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8781904.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1623..2519
                     /locus_tag="IV281_003003"
     CDS             1623..2519
                     /locus_tag="IV281_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP8781905.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            complement(2662..3603)
                     /locus_tag="IV281_003004"
     CDS             complement(2662..3603)
                     /locus_tag="IV281_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8781906.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            3729..4850
                     /locus_tag="IV281_003005"
     CDS             3729..4850
                     /locus_tag="IV281_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8781907.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            5108..6610
                     /gene="lsa"
                     /locus_tag="IV281_003006"
     CDS             5108..6610
                     /gene="lsa"
                     /locus_tag="IV281_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8781908.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            6677..7204
                     /locus_tag="IV281_003007"
     CDS             6677..7204
                     /locus_tag="IV281_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8781909.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
BASE COUNT         2464 a         1331 c         1493 g         2033 t
ORIGIN      
        1 tcattcattc gtcaagacga aagcatcatt ctttttttga taactgaaat aaatgaacag
       61 gtaaagcaaa ccggtcaata cagttacccc aatggagttc gtgaatagga taccaaggaa
      121 aatcaaccat aagactgcta atgcaaaaaa gctgattcct ttgtcccata acgttccttt
      181 ccttagtgtc agtacaaagg acgcagcact gataatgatc cagatcaata atactgtata
      241 ggaaagagaa cctgccagat agccaaataa tttgtcaccg acaaaataag aaagagccac
      301 acctagataa agaacactgg tacaaaacag cacagcacgt tgcggtactt gatgcttgtt
      361 taaaacagct agttttctag aggaaccttt ccgatctttc aagcggaaga acaaaattct
      421 cgaagaagca tatacacctg aattgataga cgagaacaga gcaagaacaa tcacgaaatt
      481 gacgatatct gccgcaaatg gaatattcat tttttcaaat accatcacaa aaggactaac
      541 gttcgtgcct gctaaagtat tccaaggaaa aataatcaaa agcaaaaata aaggaataat
      601 gtaaaaggaa atgattcttc ccatgacgcc tttgattgct ttaggaatcg cacgtttagg
      661 atcatctgct tcgcttactg tgatagcaat caactcagaa ccgccgtaag aatagataac
      721 tacaagcaaa gaactgatga ttcccttcat accgtgggga gtaaaaccac catgatcagt
      781 taattgttga agggtaggga aaacaccagt acccaacatt tgctgaccga caagataagc
      841 agcaaaaatg atcaatagga taatgacact gattttagca aaggccagcc aatattctgt
      901 ctctgcgaat aatcgtaccg aataaagatt gatgatagta gttaaaacag ccaacataaa
      961 aacaaatacc caagctggga tgtgggggaa ccacaactgt aaaaaggaag cagctgcaac
     1021 agcttcagca atgatattga taatccacat ggaccagtag acccaatcgg taaaatcggc
     1081 agcatgagca cctagatatg gacgaatcaa tccagataat ccgtggtgcg tatcaccacc
     1141 acttaaaaca atttttccca gacctttcat cacgatatat aaaattatgc cgccaatcaa
     1201 aaaagcgatc aatacagaag ggccggcgat tccaatcgct tcgccacttc ctttaaatag
     1261 acctgcacca atcgctccac ctaaagctag catggtgata tgtcgggagc taagttgttt
     1321 tttcaatttt ttatcttcca taaataacct ctttctttta aattcacaat caaaatgcat
     1381 agtaagaata aatagtaaga ataaatagta ataataataa tgactaatga taacaaattt
     1441 tcagataatt acaactattc agaaaacttt tgggcatatt ttaatggaag aatgatttat
     1501 ttcatcgtat tttcatagaa atgttaaatt tttataaaaa taataggaag cgttttactg
     1561 gctttttact atttaaacag gctatcatag gaaaaagtga cagagaaagt gaggcggaac
     1621 agatgaacaa aaacgacgta ttgctaattg cgaaaaataa atcactttat ttttttcctt
     1681 ccatccgctc tttagatgtt tggtccggaa aagatttact acagtcggaa ttcattaaat
     1741 cagctcgaca acagttggca aattatcagc tgatcgtttt tttagattat ggatttgata
     1801 tggagatggc tcgacgcgtg cgtccattta ccaatgcaaa aatcgtgtta tttttttgga
     1861 accactttaa agcagaacac gaggcgatgc tgaagcaggc acaagatgaa ccggcaatcg
     1921 atgagattta tcattttgat tttttagaag cgaaagaact tggactaaaa cataatagtt
     1981 ccttttattc gaaacatatg gatttttctt ctagcgaacc aaagacggat ctattttttg
     2041 gtgcaacaaa taacggcaga aaagaacggg cagaaaaata taagcaggaa tttacaaaaa
     2101 gaggtgttac gaccaattac tttattcttc cgcttcgagg aaatgaacaa cctggatacc
     2161 ttacttatga agaatatctg aaaaaaaccg gggaatcgag ggggattcta gaactgttgc
     2221 gggaagggca gcaaggagtg actttgcgaa cgtttgaatc tatgtatttt caaaagaaac
     2281 tagtgacaga taatcaagcc atagctcatt atcagtttta tcattctgaa aatatttttt
     2341 tgcttcaaga acgtgattta gatgaattac cggaattttt gcatacgcca tttcaaccaa
     2401 tcccatcaga actgttggaa ttttttgatg cagaaaattg ggcaaaacgt tttttcacta
     2461 ctgactccgc tatttatgaa caatatgaat acgatctttc ctctgttaaa aataaataaa
     2521 agtgccaacc aatcatagat gaaaataaac aagcgcaatt cttatggcaa catgagaatt
     2581 gtgtttttct ttttgaactt tcatgggggt aaaaaaatga gaaccagctg ctgatccagc
     2641 tgattctcat cagagagcct tttattctgc gccaaggatt ccttcttttg catggactaa
     2701 ttgggtcaaa aaggcacaat atggtgtagc aatatcatat ttttgtcctt ttcgccaaac
     2761 ggcaccattg atgtagtcga tttcagtcaa acgatggttt ttgatcaggt cttggtacat
     2821 agatgggtaa tgcaacccga tgttgtcagg atcataagta gaagcaatat gttggtacac
     2881 ttcttcttga tctaatgtga ttccttcttt tgctgcgata gaagcaaact cagatacaat
     2941 cgttcgaacc atgccctctg ccgctttttg cgcacctaat tcagcaatat tacaatccaa
     3001 tatcgtgcat aatccattta atgttccgtt tacacaagct tttcgccaaa tggaataacg
     3061 aacattatga ctatagattg gattcaattt tgcttcttga aagacttcta caactttttt
     3121 tgtaaatgct tcgccttctg gttccaagtt ctctagttcg atttctccat cacctaataa
     3181 tgtgactttt ccgggaccag ctagaccagc tgtccacatc gtgatcccaa ataagatatt
     3241 tttctttggt acatattttt ccagtacatc ttcatgacct aatccattca gtaaacacaa
     3301 aacataagta ttctcagtaa taatggattg gatcgaacga aacatatcat ctaattgatt
     3361 cgcttttgtc aaggctacga tcaaatccac gtgtttgttg ctttcgatga tttcttctgg
     3421 tgaatagatt ggcagttttg caactacttc tttgccatta aagtcagcaa tcaatccatt
     3481 tgtacgaatg gcttcaatgt gagcaggcca gcgatcgatc aagacgacct catttccgct
     3541 ttgatgcaac attaatccga ttcggctgcc cattgctccg gctccagcta ttgcgatttt
     3601 catgtttttt ctctccttta ttcaatgagt tcccttttag tatatagaaa aacagaagaa
     3661 aatagcattg ttttgtttga aaaaagtgaa aaaaagtgtc ttacttaaga agaaagtgag
     3721 ggatatagat ggatattttg gcggcaatcg attcatttaa aggaagtgct tcttcatttg
     3781 aattaaatca ggcagttcta aatggttggg aagaccgtaa aggaagaaaa atcaatgtgc
     3841 cgattgcaga tggcggagaa aatactgcag aagcaatcta ttacgcgctc gggggaacct
     3901 ggcgtacgat tgatgggatg gatctcttat ttcgtcagcg aaaaatcagt tatttattga
     3961 cgaactggca aggaaaaaat accgcagtca ttgaatctgc acaagtatta ggattagatt
     4021 tacttgataa accaacggat caaaccatcc gacttgcttc tagttatgcc ttgggagagc
     4081 ttatcaaaga tacactgctt caagaagtgg aacaaatcat tgtcacttta ggaggaagcg
     4141 gatgtagcga tggtggtcta ggattactcc aatctttggg tgcttcttta gaagggtaca
     4201 cagaaggaaa tcctttgtta tctactagaa aacttcattt ggatcgaagg aacgttcttg
     4261 tttctaaagt aaaagtgatc atcgctgcag acgtgaccaa cccatataca ggtcaacaag
     4321 gagctgcttg gcaatttgga agacaaaaag gaggaacgga tacgacgtta gctttcttgg
     4381 ataaacaagc tgaaaagatt gctcagcaga taaaagatgt ttgggaggtg gatttagatg
     4441 acatcccagg aagcggagca gcaggcggta ttggcggagc cctttttttg ttaggagcga
     4501 agatggtctc tggttttgag ttggtcagcc aaatcactgg attagaaaaa cagatcgaac
     4561 aagcggatgt catcattaca ggagaagggc ggattgatca gcaaacaatc cacggaaaag
     4621 ttccatatgg cgtagcagtg cttgcaaaaa aatatcgaaa accaataatc gctatttgcg
     4681 gaagtagaga ccgagagctg gaaggactct ttgattttct ccccattgtc ttaagcattc
     4741 aattaggacc aatcgattta gaggaagcaa tggaacacag gcaaacatta gaaaaaacag
     4801 aaatcttagg acagacactc agcagatttt tcaacattct cccgaagtag ttgacaaaaa
     4861 caatttcttt atgctatcgt aatttcaaat ataggacaaa gtgagggatc cgtgcattga
     4921 aaaattaagt cgattgatga aagctgcatt cagactgtac cttgtggaga caagggagca
     4981 gtgtgcagat tttcgggatt ggcttggttc tttcgatgta tgggtctttt tgtgtgcccc
     5041 aacccttgca tcctctttag ccagttctga aaataggact ggtctttttt tacgaggtga
     5101 tggaataatg tctaaaatcg aaataaaaaa tctgacattc ggctatgaca gccaaggcac
     5161 attattattt gaacaagcca atctaaactt tgacacacaa tggaaactag gacttatcgg
     5221 acgaaacggt cgaggaaaga caactttact gaatatcctg caaaacaaac tcccttatca
     5281 agggcaagtg atccatcagc aagcgtttgt ctatttcccg cagcaaacaa aagataaaga
     5341 gcgtttgacc tattatgtgt taaatgagat tatggatttt gagatatggg aaattgaaag
     5401 agagctccaa ctgatgcaaa cagatcctga aatcttatgg agagaattca gcacactatc
     5461 aggaggagag aagacaaaag tcctactggc actcttattt gtggatgaca ctcatttccc
     5521 gttaatcgat gaaccaacga atcatttgga tatctctggc agaaaacaag tagcggctta
     5581 cttgaaaaag aaaaaacaag gattcatcgt ggtgagtcat gatcggggat ttatcgatga
     5641 agtagtggac catgttttag caatcgaaaa gagccaactg gaactttatc aagggaattt
     5701 ctctatctat gaagaacaga aaaaacttcg tgatgaattt gaaatagcta aaaatgaaaa
     5761 attgaaaaaa gaagtcagca ggctaaagaa aacagcagct gaaaaagcag aatggtctcg
     5821 ttcccgagaa ggggacaaaa caaagaaaaa agtcggattc atcgatacgg aatctaggcg
     5881 agtgaataaa ggagcagtag gcgctgatgc tgcacggacg atgaaacgat ctaaagcaat
     5941 cgtgaatcgg atggaaaccc agatcagtga gaaagaaaaa ttattaaaag atatcgagta
     6001 tatcgatgct ttgacgatga atagccaagt gtctcatcat aaacggctgt taagcgtaga
     6061 aaatcttcaa ttagggtatg aaaatctgtt attcgagcca gttcatttta cgatcgaacc
     6121 tcatcagcgg atggcaattt caggtcctaa cggcgcggga aagtcatcca ttatccatta
     6181 tcttctgggg acattcaacg gcaaggttgt aggagaaaaa tcccagccca aacatttgac
     6241 cattagttac gtacgccaaa attatgaaga caatcgaggg acattggcgg aattcgcgaa
     6301 gaaaaaccga gtagactatc aagcattttt gaataatctc cgaaagcttg ggatggaaag
     6361 agatgttttt cataataaaa tcgaacagat gagtatggga caacggaaaa aagtggaatt
     6421 ggctaaatct ttatcccaac cagctgagct ctatatatgg gatgaaccat tgaattattt
     6481 ggatgtcttc aatcaagaac aattagaaca actgattttg aacgtgaaac ctgctatgtt
     6541 actagtggaa catgatcaaa cctttctaga taaagtatct actgagatta tttctcttga
     6601 gaaaatctaa accttgtaat ctcaaagaca acaaggtatg gttaaggaga actttgtcaa
     6661 cgaaggagag aatcacatgc tttatgtaac gagacacgga gaaacaactt ggaacgcaca
     6721 aggattagtt tgcgggcgag cagatgtgcc gctgactgaa aaaggacaga tgcaggcaca
     6781 ggcactagca gaaaaggtag tagatttgcc tgttccaatc acgaaaatca tccattcccc
     6841 tcttcaacga gcaagagata cagcacaagc agtagttgat cgtctcaatt tgccgatgac
     6901 cgctgatgag cgattggtag aaatggattt tggggattat gacggactgc caagcaaaga
     6961 tgaaaacttt caaaaagcgc gtttagcgtt tgctgtacgt ttcccaaatg gagagtcggt
     7021 cttagatgtg tacgcacgga tcgttccttt attgaaagaa tgcatggaag acgaagaaaa
     7081 tgtctacttg ttagtctgtc ataatgcctt gataagagtg atcaatgctt attttcatcc
     7141 aatgcctaat gaagggttct ttacattcat ggtagacaat acagaattaa tttcttatga
     7201 gtaattagac ttttgtgtta tttaaataaa atgtttcaag gcactgcttt tgcttagcag
     7261 tgcctttttt gtggctgtgt caattctttt ggcattataa atccaatttt tagaaataaa
     7321 a
//
LOCUS       DACDIH010000285         2900 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.guided.143, whole genome shotgun sequence.
ACCESSION   DACDIH010000285 DACDIH010000000
VERSION     DACDIH010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.guided.143"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1418
                     /locus_tag="IV281_003008"
     CDS             <1..1418
                     /locus_tag="IV281_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8781910.1"
                     /translation="KISTLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLE
                     NYLKQYRGALLMVTHDRYFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVE
                     QEEKRKRLYKQELAWMRAGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQR
                     LGKKVLEIKDGSYRINDQTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLD
                     SGTMSIGETVHLAYYTQENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLF
                     PRFMHGTLIGKLSGGEKRRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFP
                     GAVITVSHDRYFLDKVADKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQ
                     SKEPAEKKKLSYMEQKEWETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSE
                     TEAQLEEKMARWEYLSEWVED"
     gene            1430..2377
                     /locus_tag="IV281_003009"
     CDS             1430..2377
                     /locus_tag="IV281_003009"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8781911.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            2397..>2900
                     /locus_tag="IV281_003010"
     CDS             2397..>2900
                     /locus_tag="IV281_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8781912.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKS"
BASE COUNT         1000 a          495 c          654 g          751 t
ORIGIN      
        1 aaaaaatcag tactttatca ggtggtcaga aaaaacgagt tagtctggcg caagtgttga
       61 ttgatgaacc cgatttacta ttactagacg aaccaacaaa ccatttagat tatgaagcga
      121 tcgagtggtt ggaaaactat ttgaaacaat accgtggcgc actattgatg gttacccacg
      181 atcgttattt tctagataga gtagcaaacc gaatcttcga actttcattt ggaaaattat
      241 acgagtataa aggaaattat gaagcttacg tcttagaaaa agcagaacgt gatcgtgtag
      301 aagtagaaca agaagaaaag cgcaagcggc tttataaaca ggaattagct tggatgaggg
      361 ctggcgtaca ggcacgtggc accaagcagc aagcacggat caaccgtttc gaagatctaa
      421 aggagaactt gtaccaagta aaccaagaag atgatttaga gctgaaccta gcgacacaac
      481 gtttagggaa aaaagtactg gaaatcaaag atggcagtta ccgtataaat gaccaaacac
      541 tgctagaaca tttagattta ttgattcaat ctcgggaaag actaggtatt actggtaaaa
      601 atggtgcggg aaaatcgaca ttgcttaata ttctagctgg cagaattccc ctggatagcg
      661 gtacaatgtc tatcggtgaa acggttcatc ttgcgtatta tacacaagaa aatgaagaaa
      721 tggcaccaga taaacggatg attgcttact tacaagaagc tgctgaagaa gcaaaaacag
      781 cagatggcag tcaaatcgga gtagcagaat tacttgaacg atttttattt ccaagattta
      841 tgcatgggac actgataggc aaactttcag gcggagaaaa acgccggtta tttttgttga
      901 aactgctgat tcagcaacct aatgttttat tattggatga accgaccaat gatttggaca
      961 tagcaacatt gacgattttg gaagattatt ttagaagttt tccaggtgct gtgatcactg
     1021 tttcccatga ccgttatttc ttggacaagg tagcagataa gttattagta ttccaaggaa
     1081 acggaaaaca agaactttat tacggaaaca tgtccagtta tttactgaag caaaaagaga
     1141 cccagcagcc tgctgaaaaa gcgaaaccaa aaatacagag caaggaacct gctgaaaaga
     1201 agaaattatc ttacatggaa caaaaagaat gggaaacgat tgaagatgag attgcagaac
     1261 ttgaagaaaa aatctcattg ctccaagaag agatgaatca tcaaggagat aatttcactc
     1321 gattacaaga attgcaaaac gacgtatctg aaacagaagc acaacttgaa gaaaaaatgg
     1381 cacggtggga atacttgagt gaatgggtag aagattagga ggaaaaagga tggaacaagc
     1441 gtatcttgac ttaggaagaa aattgttgga agaagggcat cgtaaagaag acagaacagg
     1501 cacaggcaca ttaagcttat tcggatacca aatgcggttt gatctgcaaa aagggtttcc
     1561 tttgttaaca acgaaacgag taccttttgg attgatcaaa agcgaattgc tttggttttt
     1621 aaaaggagat acgaatatcc gctatctatt acaaaataaa aaccatattt gggatgaatg
     1681 ggcatttgaa cgctacatca aaagtccaga ctataatgga ccagatatga ctgattttgg
     1741 ccatagatgt ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga gcaagctttt
     1801 ttgcgagaag atcctaaccg atgattcctt tgctgaaaca tacggcgatc ttggacacat
     1861 ctacggctat caatggcgtc actgggaaac aaaagacggt ggctttatcg atcaaatcaa
     1921 acaagtgatc gaagcaatca aaaagactcc tgattctaga agattgatcg tttctgcatg
     1981 gaatccggaa gatgttccta gcatggcttt gcctccttgt catacgatgt ttcaatttta
     2041 tgttcaagag ggacggctaa gctgccaatt gtatcaacgc agtggggatg tgtttctagg
     2101 cgttccgttc aacattgcaa gttatgcgtt gctgactcac ctaattgcac atgaaacagg
     2161 tttagcggtt ggcgaatttg ttcacacatt aggagatgct catctttata tcaatcatat
     2221 cgaacaaatg aaagaacaat tgagccgaga aatacgcgca tttccgactt tagttttgaa
     2281 tcaggaaaag cagtcagtat ttgattttga gatggaagat atccaattag aaggatatga
     2341 cccacatccg gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat gagaaaatgt
     2401 tagctgcaat ttgggcacag gatgaaaacg gattgattgg aaaagaagac cgtcttcctt
     2461 ggcgtttgcc aaatgatctg aaattcttca aacaaatgac agaagcaaat acactagtga
     2521 tgggacgaaa aacattcgaa gggatgggta gccgtccgtt gccgaatcgt cagacgattg
     2581 ttttgacgag agattcttcc tataaagcag aaggcgtgca tgtcatgcat agtgtggaag
     2641 acgtgcttgc tctggaaaaa gaaacagacg gtatcttttt catcgctgga gggtcagctg
     2701 tttatcaaga atttctgcca ttttgtacga tattataccg tacagtcatc catcatgtat
     2761 tcgatggaga tgcttatttt ccacctgttg actggtctga ttggtcactg atcaatacta
     2821 gtcaaggaga aatggatgaa aaaaatcgtt atccgcatca atttgaaacg tatcagcgta
     2881 atgagaatcc tgtaaaaagc
//
LOCUS       DACDIH010000286         2454 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.guided.151, whole genome shotgun sequence.
ACCESSION   DACDIH010000286 DACDIH010000000
VERSION     DACDIH010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.guided.151"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            174..260
                     /locus_tag="IV281_003011"
                     /pseudo
     CDS             174..260
                     /locus_tag="IV281_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance protein"
     gene            261..2201
                     /gene="tet(S)"
                     /locus_tag="IV281_003012"
     CDS             261..2201
                     /gene="tet(S)"
                     /locus_tag="IV281_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(S)"
                     /protein_id="HAP8781913.1"
                     /translation="MEEIKLKIINIGILAHVDAGKTTLTESLLYSSGAIKELGSVDSG
                     TTKTDTMFLERQRGITIQTAITSFQRENVKVNIVDTPGHMDFLADVYRSLSVLDGAIL
                     LISAKDGVQSQTRILFHALRKMNIPIIFFINKIDQNGINLPDVYQDIKDKLSDDIIIK
                     QTVNLNLKPYVIDYTEPEQWETVIVGNDYLLEKYTIGKTLNIAELEKEENERIQSCSL
                     YPVYHGSAKNNIGIKQLIEVITSKLFSPTQLNSDKLCGNVFKVEYSDDGQRLVYVRLY
                     SGTLHLRDSVNISEKEKIKVTEMYTSINGELRQIDKAEPGEIIILKNELLKLNNVLGD
                     KKRLPHREILENPLPMLQTTIEPCKSVQREKLLDALFEISDSDPLLQYYVDTVTHEIV
                     LSFLGEVQMEVTCTLIQEKYHIEIETRKPTVIYMERPLKKSEFTIDIEVPPNPFWASI
                     GLSVTPLPLGSGIQYESLVSLGYLNQSFQNAVMEGIRYGCEQGLYGWKLTDCKICFKY
                     GLYYSPVSTPADFRMLAPIVLEQAFRKSGTELLEPYLSFEIYVPQEYLSRAYNDASKY
                     CANILNTKLKGNEVILIGEIPARCIQEYRNSLTFFTNGRSVCLTELKGYQVTNIKSAF
                     QPRRPNNRIDKVRHMFNKINLH"
     gene            2289..>2454
                     /locus_tag="IV281_003013"
     CDS             2289..>2454
                     /locus_tag="IV281_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8781914.1"
                     /translation="MCPICGNKTRLKMREDTELKNFPLFCPKCRQEILIEITKFRITV
                     ITEPDAKTQSR"
BASE COUNT          893 a          356 c          449 g          756 t
ORIGIN      
        1 aagataacat tcaaaaggag ataataaaaa tgcggcaagg tattcttaaa taaactgtca
       61 attagatagc gggaataatt aattaagcat ccttatttaa aataaggact ggttttttgt
      121 gcccagttta agaatacttt tatcatttta tcataaagca tctgtggcta tcagtgtgta
      181 gtatacagct atttttgtat taaaatccca gtgataagag tatttatcac tgggattttt
      241 tctgcttttt gagcttttga atggaggaaa taaaattgaa aattattaat atcggtatct
      301 tagcacatgt tgatgcagga aaaactactt tgacagaaag cttactatac agtagcggag
      361 caattaaaga gttaggaagt gtagatagcg gtacaacgaa aacggatact atgtttttgg
      421 aacgccagag aggtattact attcagaccg caataacatc ttttcaacgg gaaaatgtta
      481 aagtaaatat tgtagatact cctggacaca tggatttttt ggcagatgta taccgttcat
      541 tatctgtttt ggatggagct attttgctaa tctctgcaaa agatggagta cagtcacaaa
      601 ctcgtatact attccatgca cttagaaaga tgaacatacc tataatattt tttattaaca
      661 aaattgatca aaatggaata aatttgccag atgtttatca agatattaag gacaaacttt
      721 ctgacgacat cataattaag cagactgtga atctaaattt gaaaccttat gtaatagatt
      781 atactgaacc agaacaatgg gagacagtaa ttgtgggaaa tgattattta ttagaaaaat
      841 ataccattgg gaaaacattg aatattgcag aacttgaaaa ggaggaaaac gaaagaattc
      901 aaagttgctc cttatatcct gtttatcacg gaagtgcaaa gaataatatt ggaattaaac
      961 aacttataga ggtaattact agcaaattat tttcacccac acaactcaat tcagataaac
     1021 tttgtggaaa tgtttttaaa gtagaatatt cagatgatgg tcaacggctt gtctatgtac
     1081 gtctttatag tggaacgcta catttgcgag actcagtcaa tatatcagaa aaggaaaaaa
     1141 taaaagttac agaaatgtat acttcaataa atggagaatt acgccagata gataaggcag
     1201 agcctggtga gattattatt ttaaaaaatg agcttttaaa actaaataac gtacttggag
     1261 ataaaaaaag attaccacat agagaaattc ttgagaatcc tcttcctatg ttacaaacaa
     1321 caattgaacc atgtaaatca gtacaaagag aaaagttact agatgcactt tttgaaatat
     1381 ccgatagtga tccccttcta caatattatg tagatacagt aactcacgaa attgtgctat
     1441 cttttttagg tgaggtccaa atggaggtaa cttgtactct gattcaagaa aaatatcata
     1501 ttgagataga aacaagaaaa ccaactgtca tttatatgga aagaccatta aaaaaatctg
     1561 aatttaccat tgatatcgaa gtacctccaa atcctttctg ggcttctatt ggtttatctg
     1621 taacaccact tcctttgggt agtggcattc agtatgagag cctggtttct ctaggttatt
     1681 taaatcaatc atttcaaaat gcagttatgg aaggtatacg ctatgggtgt gaacaaggat
     1741 tgtacggttg gaaattaaca gactgtaaga tctgttttaa gtatggtcta tattacagcc
     1801 ctgtcagtac gccagcagat ttccgaatgc ttgcgcctat tgtactagag caggctttta
     1861 gaaagagtgg tacagagtta ttagagccat atcttagctt cgaaatttat gtaccacaag
     1921 aatatctttc gagagcatat aatgatgctt ccaaatattg tgcaaatatt ttaaatacta
     1981 agttaaaagg taacgaggtc attctcattg gtgaaattcc agcccgttgt attcaagagt
     2041 atcgaaacag tttaactttc tttacaaatg gacgcagtgt ctgtttaaca gagttaaaag
     2101 gttatcaggt tactaacatt aagtctgctt tccaaccacg tcgtccaaat aatagaatag
     2161 acaaagtaag gcatatgttt aataaaatca acttacattg atagtgaact aaagagaaat
     2221 ttttaaaatg aatattgagt tgttaattag aacaggagga tatagatatt gtaaatacta
     2281 ggtggatact gtgccctata tgtggcaata agacccgttt aaaaatgagg gaagatactg
     2341 aattaaaaaa tttccctcta ttttgtccta aatgcagaca agaaatttta attgaaataa
     2401 cgaagtttag aataactgta attacagagc cagacgcaaa gacgcagagc cgat
//
LOCUS       DACDIH010000287         1688 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.guided.175, whole genome shotgun sequence.
ACCESSION   DACDIH010000287 DACDIH010000000
VERSION     DACDIH010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.guided.175"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            42..1454
                     /gene="msr"
                     /locus_tag="IV281_003014"
     CDS             42..1454
                     /gene="msr"
                     /locus_tag="IV281_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8781915.1"
                     /translation="MDSLRVYQQDRIGIIGENGVGKSTLLKLIAGELFPDHGKIQTEI
                     TFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERKWSGGEERKIELIRLLSSYEQGM
                     LLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRYFLDELASKIWEVKDGEIREFSG
                     NYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQEKKKQAEKLEKVSSKKKKQQIRP
                     DRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKPQQMKQIRFPVPKSLELHSRYPI
                     MGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGSGKTTLLEHIRKQGEGILLSPKV
                     SFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSLLNHLGFAQETLTKPLCTLSGGE
                     ATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEKLMQIYPGTILFTSHDSYFVERT
                     ADEVYEIKGQKIKKVLTRNF"
     gene            1501..>1688
                     /locus_tag="IV281_003015"
     CDS             1501..>1688
                     /locus_tag="IV281_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8781916.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLA"
BASE COUNT          574 a          291 c          368 g          455 t
ORIGIN      
        1 caaatctaca agtcagcttt ggcaaccagc tagaactatc tattgattct cttcgtgtct
       61 atcagcaaga tcgtataggg atcataggag aaaatggtgt cggtaaatcg actttgctca
      121 aactaatagc cggtgaactt tttcccgatc atgggaaaat ccaaacagag atcaccttca
      181 actacctgcc tcaattaacc tatctcgctg aagcaaagga cctaaatttg gaattagcca
      241 gtcatttcca gttaagactg gaagaaactt cagagcggaa atggagcgga ggggaagaac
      301 gaaagatcga gttgatacgt cttctttctt cttatgaaca agggatgctt ctagacgagc
      361 caacaaccca tctagataga aaaagtattg atcgactgat tgaagagctt cgttattatt
      421 atggtacgct ggtttttgtt agtcatgatc gctattttct agatgagcta gcatcgaaaa
      481 tctgggaagt aaaagacgga gaaatccgag agttttcggg gaattatagt gcctatctca
      541 ctcaaaagga attggagaaa aagactcagc tacgagaagc agagtcgatc atgaaagaga
      601 aaaaacgatt ggaaaaatcg atccaagaaa agaaaaaaca agcggaaaag ttggaaaaag
      661 tgtccagtaa aaagaaaaag caacaaatca gaccggatcg gttgtcttcc tctaaacaaa
      721 aagacagtgt acaaaaagcc atccaaaaga atgcgaaaac attagagaga agactccaaa
      781 aaataggaga aacaaccaag ccgcagcaga tgaaacaaat ccgttttcca gtaccaaaat
      841 ctcttgaact ccacagccgc tatcccatca tgggacaaaa tgtccaattg gaaagaagtg
      901 ggagaacatt actggtaaat ggtgattttc agttttcttt aggtaaaaaa atcgcgattg
      961 tcggtgaaaa tggttcaggt aagacaacct tattagaaca tatccgcaaa caaggagaag
     1021 gaatccttct ctctccgaaa gtaagctttc aagtatatca gcaaaagggt tatcaaatga
     1081 catctgaaga atccatcatt cgttttgtca tgagacaaac agagttttca gaatcgcttg
     1141 tccgtagttt gctgaatcac ttagggtttg ctcaggaaac tttgacgaaa ccgttatgta
     1201 cattaagtgg gggagaagcg acccgtctga cgattgcttt gctttttact aagccaagta
     1261 atgtgttgct gttagatgaa ccgactaatt ttattgatat ggcaacgatc gaagctttag
     1321 agaagctaat gcaaatatat ccgggaacga ttttgtttac ttctcatgat tcttactttg
     1381 tcgagcgtac ggctgatgaa gtttatgaaa taaaagggca gaaaataaaa aaagtactta
     1441 cgagaaattt ttaaatgtgc tacgatgatt ttcgtgacaa atttaaagga gtggtacaga
     1501 ttgcctacaa ataaaaaaga aggaataatt tttacaacaa tgatgtgttt tttgatggtc
     1561 ttggggatga gcgcgtataa tttatggctg catcaagatc ttcatttatc aagtttgttg
     1621 attggtctag tgcctggatt cgtggtggct ttcattttgg atgtcttcat catcggtgtg
     1681 ctggcgaa
//
LOCUS       DACDIH010000288          671 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E63705_2016
            SAMN12640289-rid9887963.guided.267, whole genome shotgun sequence.
ACCESSION   DACDIH010000288 DACDIH010000000
VERSION     DACDIH010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640289
            Sequence Read Archive: SRR10040960
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742904.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742904.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 20:45:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,950
            Genes (coding)                    :: 2,866
            CDSs (with protein)               :: 2,866
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 43 of 84
            Pseudo Genes (incomplete)         :: 30 of 84
            Pseudo Genes (internal stop)      :: 26 of 84
            Pseudo Genes (multiple problems)  :: 11 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640289-rid9887963.guided.267"
                     /isolate="E63705_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            58..606
                     /gene="aac(6')"
                     /locus_tag="IV281_003016"
                     /pseudo
     CDS             58..606
                     /gene="aac(6')"
                     /locus_tag="IV281_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
BASE COUNT          244 a          106 c          151 g          170 t
ORIGIN      
        1 aacatttcgt tttttcaaaa ggactgttag aataagcaag aacaaggggg aagacgtatg
       61 ataatcagtg aatttgaccg taataatcca gtattgaaag atcagctttc tgatttactg
      121 agactgactt ggccggaaga atatggagac agctaggcag aagaagtaga agaaatgatg
      181 aatccagaac gaatcgcggt agcagcggta gaccaagatg agttagtagg atttattggt
      241 gcaatccctc aatacggtat cacaggttgg gaattgcatc cattagttgt agaaagctcc
      301 cgacgaaaga accaaatagg tactcgatta gtcaattact tagaaaaaga agtagcttcc
      361 agaggaggaa tcacgattta tttaggtacg gatgatttag accatggaac aacgttaagt
      421 caaacggacc tgtatgagca tacatttgat aaagtggctt ccatccagaa ccttcgtgaa
      481 catccgtatg aattctatga aaaattaggt tataaaatcg taggtgtctt accaaatgca
      541 aatggctggg acaaaccgga tatttggatg gcaaaaacga ttattcctcg accaaattct
      601 caataataaa aagaggttgt gacagaagta atcagcttca agaaataaga aggaatttac
      661 gaaaattact t
//
